LOCUS       DAOSOU010000001        31745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_1439_length_31745_cov_6.657021, whole genome shotgun sequence.
ACCESSION   DAOSOU010000001 DAOSOU010000000
VERSION     DAOSOU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 31745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31745
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1439_length_31745_cov_6.657021"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PLW15_00005"
     CDS             <1..1179
                     /locus_tag="PLW15_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32812.1"
                     /translation="LIVFGRTGTATYRLSTESDLIQLPVIPADVQAVSIGITVLMVAI
                     TAVAFWFTWRARPTPLWLVSIFAVLFVIGFLTWAAAGALIPIPGLLVGAVGLSVPLIF
                     GALGGVISERVGVVNVAIEGQLLAGAFVSAVVASVIKDSFFGPLLGLLAAVVAGVLVA
                     FVLAAFSIKYFVDQVIVGVVLNVLVVGLTSFLFSKVLAPNAALLNSPPRFPRLPIPLL
                     SEIPILGPVLFRQTIIVYIMYVAVAVVWFALFKTRWGLRLRSVGEHPQAADTVGINVN
                     STRFWNVSLAGAIAGLGGAYFTLGSVGAFNKEMTAGAGFIALAAVIFGRWDPIRATMA
                     ALLFGFASNLQGVLSAIGSPVPSEFMLMLPYVVTILAVAGFVGHVRGPAASGKPYIKS
                     "
     gene            1176..1601
                     /locus_tag="PLW15_00010"
     CDS             1176..1601
                     /locus_tag="PLW15_00010"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT32813.1"
                     /translation="MTDYLPKTDADWDALRGVAKEALSHAYVPYSNFPVGVAAIVDDG
                     RVISGANVENASYGLTLCAECALVSSLHMTGGGKLVAFTCVDGHGNALMPCGRCRQLL
                     FEHSAEGMLLETVSGIRTIDEVIPDAFGPRTLEAYRATD"
     gene            1635..2921
                     /locus_tag="PLW15_00015"
     CDS             1635..2921
                     /locus_tag="PLW15_00015"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOT32814.1"
                     /translation="MAVEPFDVVDLIRTKRDKGVLTTEQITWLVDAYTRGYVADEQMS
                     AMTMAIFLNGMERTEIRDLTLAMIATGETLSFDGLGKPTTDKHSTGGVGDKITLPLAP
                     LVASFGVAVPQLSGRGLGHTGGTLDKLESIPGWRAKLSNDEFYAQLKSVGAVICAAGS
                     GLAPADGKLYALRDITGTVEAIPLIASSIMSKKIAEGTAALVLDVKFGSGAFMQDQAR
                     SRELAETMVQLGKDAGTKTVALLTNMNVPLGLAIGNANEVRESVEVLAGGGPADVVEL
                     TIALAREMLALAGQPDADVEAALKDGRAMDTWRAMIAAQGGDPDAALPAPRESQVVTA
                     DADGVLAAQAALPFGIAAWRLGAGRARKEDPVHHAAGIDLHAKPGDRVTKGQPLFTMH
                     TDEPARFERALEALEGAYRIGDTADDVGPLIAGRVD"
     gene            complement(3026..3991)
                     /locus_tag="PLW15_00020"
     CDS             complement(3026..3991)
                     /locus_tag="PLW15_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32815.1"
                     /translation="MDDSRVAGRWSCIVGGVTARIPLPDALGAAFSVASALQLGIGPR
                     RLAGADLERPFWGVRAAAGSLDDLTSVARAYSARMPGHAFFSHATAAQLWSIPLPIHL
                     ENPLPVHVSVPSDRRAPEGRGVAGHRVEIHRADIETINGLAVTSPERTVIDLAPLLVD
                     EHLLGALDNIFWRRRSTASRATRISFRECLDRHRGRRGRSRVETLLPLTTDRSDSIPE
                     SAFRLRFIRAGFPDPVPNQNVYDRNGRFIAMPDLQLLEYRMAFDYEGDHHRTDARQWR
                     KDLRRVPLLEDAHWHHTRMSADDLANPRELLKRTARRLRERGWPG"
     gene            complement(4065..5009)
                     /locus_tag="PLW15_00025"
     CDS             complement(4065..5009)
                     /locus_tag="PLW15_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOT32816.1"
                     /translation="MATKIILDCDPGHDDAIAMLLAWGNPDIDLVGVTTVMGNQSIEK
                     VTRNALAVARVAGITGVPFARGSHRPLVRPVEFADTIHGESGLDGPVLPEPTLSLDPR
                     HAVDFIIDTVMASEPGEITLVPTGALTNIALAVRKEPRIASRVKQVVLMGGGVNVGNW
                     SATSEFNIVIDPEAAHIVFNESWPLTMVGLDLTHEALATPEVAAAIAAVDTAPARFVG
                     ELLEFFGHTYKDEQGFDYPPVHDPCAVAFVIDPTVMQAVRVPLDVELTGTLTLGMTVA
                     DFRAPAPDDCTTQVARNLDHAKFWALVVDALERIGDPA"
     gene            5071..6261
                     /locus_tag="PLW15_00030"
     CDS             5071..6261
                     /locus_tag="PLW15_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT32817.1"
                     /translation="MTDHSASTTDLSARGAARVEWIRSRMPLLARARAEFAERLPFAG
                     HRIGMSLHLEPKTAVLLETLAAGGAEVVATGNHGSTQDDIVAFLRGQGMTVFGSRDDD
                     LDQHHRNVASVAEAKPTILLDNGADLAALAAADPTGIIGGTEETTSGGLRLRGELAGR
                     IPFPMIVINDSLLKAIGENKHAVGQSVVESFMRITNLMVNSKRFVVFGYGWCGRGVAQ
                     YLRALGGKVAVVEVDEIKAFEAAMDGFRVDTADGLAEWGQVFITATGHPGVLTAPFFE
                     KVADGAVLANCGHFPWEIDVPALRAYAIGSTVLDDAIERLGLPGGRHVILIADGRMFN
                     LAGREPKGNSLESMDLGFLLQSLSLERVATAADTLTLGAQPVPEDIDREIARRMLAAL
                     SAAR"
     gene            6261..6941
                     /locus_tag="PLW15_00035"
     CDS             6261..6941
                     /locus_tag="PLW15_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT32818.1"
                     /translation="MPRDPEVPPAPDTAYAVPALEKGLDILELLADTAGGLGQTEIAE
                     ATGRSVGQIFRVLGVLERRGYVVRDRQSGLYSFSMMTFDLAHRHPPLRGLIMLATTPM
                     RELSDRVRQSCNLSVLDAARVRVIAQVESPADFGYRVRVGAVFPLETATGEVLEGAAA
                     LTRADPLQAGITDVVAPIVDARGVTRAALTVPYVATTFSGIPVDEVVAAAEDTASEIS
                     RLLQGEPR"
     gene            6952..8073
                     /locus_tag="PLW15_00040"
     CDS             6952..8073
                     /locus_tag="PLW15_00040"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT32819.1"
                     /translation="MNAAVTEFLMPGGGASITALPKVSLHDHLDGGLRPQTIIELADA
                     IGMAVPASDAAALATWFAEKSDSGSLVEYLKTFDITTGVMQTHEGLARVAREFVLDLH
                     ADGVIYGEIRWAPEQHLSRGLTLDQTVEAVQEGLEAGAEEVRHAGGSIRVGQLVSAMR
                     HADRGLEIAELAVRHRNNGVVGFDIAGAEAGFPAGNLRDAFDYLAHHFFPATVHAGEA
                     DGLDSIRGALFDGRALRLGHGVRLAEDVHTAREDDENTYVTLGPVAQWVKDREIALEL
                     SPSSNLQTGAIEQWGDELIDHPFDLLYQLGFRVTVNTDNRLMSATSLSRELALLAETF
                     DYDLTDLAVFQLNAAASAFLPLEDREDLADRITAGFEEA"
     gene            8077..8523
                     /locus_tag="PLW15_00045"
     CDS             8077..8523
                     /locus_tag="PLW15_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT32820.1"
                     /translation="MSLPPLPASAIVIGAHAADWRAAVTLAGDALAASGAAKPEYSRE
                     MIRMIEEHGPYVVIAPGLALAHARPGPEVLADGLSVVTLAEPVSFGHPYNDPVSVILG
                     LAILTADRHLAVVAAVANIFNDSTAIADLAAATTAADVQRIMQVPA"
     gene            8520..8792
                     /locus_tag="PLW15_00050"
     CDS             8520..8792
                     /locus_tag="PLW15_00050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOT32821.1"
                     /translation="MKIVTICGAGIGSSGILKVNAERVLRKLDLEATVVAADISTVKD
                     VASDAQVILTSQEFVDAIGSTFADVIVIQNYFDTEELTAKLRSALE"
     gene            complement(8799..10433)
                     /locus_tag="PLW15_00055"
     CDS             complement(8799..10433)
                     /locus_tag="PLW15_00055"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT32822.1"
                     /translation="MSLIDDARAWRDQDPDDTTRAELDALISSAEAGDTEPLAERFAS
                     RLQFGTAGLRGELGAGPARMNRVLVTQAAAGLAAYLLSKSPSPSVVIGYDGRTNSEVF
                     ARDSAVVMAGAGVRTTLLPRHLPTPVLAFAVRHLEASAGIMVTASHNPARDNGYKVYL
                     GDFDHGSQIVPPADAEIASDIALISTGSITDLPRSTDFVVADESILEEYVRRTAELGS
                     TPAPISFVYSAMHGVGWETAGKVFAAAGFAAPALVTEQIEPDAAFPTVAFPNPEEPGA
                     MDLTLAKAAEVGADVAIVNDPDADRLAVAIPTASGWRALSGNEVGYLLGWRAAQRCNP
                     DQVLAASIVSSPALREVARSYNLDYRDTLTGFKWISRVGGLAFGYEEALGYLVDPDKV
                     RDKDGISAAVDFLSLVSELKSQGKTVVDHLADFAAKFGAFASGQISLRVDDLSRIGAM
                     MAQLRAHLPERIGTLEVDRIDDFIDGFGEFPPGDILRIHLVNGARVIVRPSGTEPKLK
                     VYLDASSTDGDATARQSAAAAVIAELDAGMRELLAG"
     gene            complement(10436..11272)
                     /locus_tag="PLW15_00060"
     CDS             complement(10436..11272)
                     /locus_tag="PLW15_00060"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT32823.1"
                     /translation="MSLKTPTNPLDDPTADPFAIAAVAAAEIAERTGVERHDIALTLG
                     SGWAKAADLIGETTATIPAFEITGFSKPALEGHVGTLRSVLLPSGKRALVIGARTHYY
                     EQHGVRRVVHSVRTAAATGATTMILTNGAGGIRETWKPGTPVLISDHINLTADSPLEG
                     ATFIDLTDLYSKRLRDLARSVDSSLDEGVYCQFRGPHYETPAEVQMAKIIGGHIVGMS
                     TALEAIAARQAGMEILGMSLITNLAAGIQKTPLSHAEVIEAGREAESRISALLATIVG
                     EL"
     gene            11304..12740
                     /locus_tag="PLW15_00065"
     CDS             11304..12740
                     /locus_tag="PLW15_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HOT32824.1"
                     /translation="MAYKFERTQRIAVLGGGPGGYEAALAAAQLGAEVTLVEREGVGG
                     SAVLTDVVPSKTLIATAEATNSIGEAADLGVQFFVRSESGRAVRPDVTVNLAAVNARL
                     LRLARQQSEDMKSQLIKAGVRIIQGEGRLDGPQRLVVSTGKGKKGTDFDEIDADTLVV
                     SVGARPRMLDSAMPDGERILTWTQLYTLDQVPEHLIVVGSGVTGAEFASAYTALGSKV
                     TLISSREQVLPGEDADAARVIEDVFKRNGMTVLSKSRADSVKATKTGVIATLADGRVV
                     EGTHCLMAVGSLPNTEDIGLKEAGVQLTDSGHIRVNRVARTSMPSIYAAGDCSDFLPL
                     ASVASMQGRTAVYHAMGDAVYPTELRNVTSNIFTQPEIATVGWSQKQIEEGLTTGIIY
                     KLPLASNPRAKMMGIKDGFVKLFTSTRSGTVIGGVIVAPHASELILPVSLAVEHRLTV
                     DQLARAFPVYPSLTGSITDAARAMHIVN"
     gene            complement(12944..14698)
                     /locus_tag="PLW15_00070"
     CDS             complement(12944..14698)
                     /locus_tag="PLW15_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOT32825.1"
                     /translation="MAPITKVLIANRGEIAVRVIRAAKDSGIASVAVYADQDRDARHV
                     KLADEAYSLDGTTSADTYLVIDKLLSVARRSGADAVHPGYGFLAENADFARAVIDAGL
                     TWIGPSPDAIEKLGDKVSARHIAEKVGAPLAPGTLNPVSGAAEVLDFVDLHGLPVAIK
                     AAFGGGGRGLKVARTREEIPELFDSATREAVAAFGRGECFVEKYLDQPRHVETQCLAD
                     SHGNVVVISTRDCSLQRRHQKLVEEAPAPFLTDEQNAALYTSSKAILKEVGYVGAGTC
                     EFLVAKDGTISFLEVNTRLQVEHPVSEEVTGIDLVREQFRIAEGGVIDYDDPKPQGHS
                     FEFRINGEDPGKNFLPSPGPVNALRFPGGPGVRVDSGVTTGDVISGAFDSLLAKLIVT
                     GSSRADALERSRRALDEFEVAGLPTVLPFHRKIVRDPAFTDSPFRVYTRWIETEFDND
                     IEPWSGSLPDGSAPPARHNVVVEVGGKRVEVSLPTKLVPTAGGGTALAAAPRRSAAAS
                     VSTVTGDTVKAPMQATVVKIAVAEGDKVVKGDLILVLEAMKMEQPLTAHKDGTVTGIN
                     ATVGATVSSGHLLLSIAD"
     gene            complement(14777..15409)
                     /locus_tag="PLW15_00075"
     CDS             complement(14777..15409)
                     /locus_tag="PLW15_00075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOT32826.1"
                     /translation="MRLYLASTSPARLATLRAAGIEPIVLDPAVDEDAVAAAAGPLPV
                     HEAVQVLARAKAEAIVGTLVGGEPIDGIIVGGDSLFEFDGAVFGKPHHPEVAIERWRS
                     MRGRSGTLHSGTWIIDHRGGVDSGGAGGVSSAGVSFADLGDEEIDAYVATGEPLRVAG
                     AFTIDSLGSAFITGVDGDPHAVVGISVALLRQLVVELGIPWHTLWNRANL"
     gene            15432..16664
                     /locus_tag="PLW15_00080"
     CDS             15432..16664
                     /locus_tag="PLW15_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOT32827.1"
                     /translation="MGDWLGRTIELDVTNIAHGGVSVARHDGRVVFVADAIPGERVLA
                     RVSSDSKKSFWRAETVEVLEASPHRQPHVWDAAALDRDPVDRAGGAEFGHIELAHQRE
                     LKRQVITDSLARMARLERDVTVEAVPGDDERAGTGWRTRVRLHVDDQGRPGPFAARSH
                     RVIPVTSLPLATAELEASAPLGERFDGYESVDVLNPSTGGVRLIVGNQKPSVITERVG
                     EREFRLADSGFWQVHRGAAATLTEAVQSAIDPELFDPQAANLDLYGGVGLLAAAVADR
                     FGPSTRITSVESDATATDFAAENLSEWLGARAETGRVERWVAALAASAGPSERERLAR
                     ATVVLDPPRSGAGRDVVEAVARLKPAQVVYVACDPVAFARDTALFADLGYHLGGLRAF
                     DLFPHTHHVEAVGTFTRT"
     gene            16739..17443
                     /locus_tag="PLW15_00085"
     CDS             16739..17443
                     /locus_tag="PLW15_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT32828.1"
                     /translation="MAQASTTEVGSDIAAPQVRVAVVDDHESVRLGLKAAFLAEGYDF
                     VLAAPTVSELVEGLDGREVDVVVLDLSLGDGSTVTDNVKRVQATGAAVLVHSIADRVA
                     YVREALAAGAAGVIPKSSATKTVLAAAATVARGEVLNNLEWATAIDADRDFAKAQLGR
                     REREILHLYASGLPLKLAAQQLGIGYSTAREYLDRIRVKYVEVGRPAPTKVDLLRRAV
                     EDGILPGLDPDGGDAR"
     gene            17433..18698
                     /locus_tag="PLW15_00090"
     CDS             17433..18698
                     /locus_tag="PLW15_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT32829.1"
                     /translation="MPASATSVAAPPQSKQPRNPISRKQVENVFARSVAVFGLVFGAQ
                     TIPWMLGQLDEAYPLWLWIVVPSLFGMLVVVLITSFAKVWVKPAQGLFAILYLAALIT
                     WPLTVLPGAEIFSGIHWLNYLITVATAMAAIAFRPLIATIYLFLCPTIYVLFRATPIG
                     GGAPPLLALFEGIYAVILGGAIIIILTMLRQAASSVDNAQATALDRYAHAVRQHATEV
                     ERVQVDSIVHDSVLTTLISAARAFTPQAQHLAATMATNTIGYLRDAAAASPDDGSMVR
                     LTSLAERITETVNGYSGVFELRARSVGTRSIPAQAAEALYSAAAQAMVNSSRHAGDTG
                     VTRWVAFRSVAPGGIEIIVGDTGRGFSVADIPEERIGVRISIIERLANAGGRAVIQSA
                     PDEGTLVSLRWPNPAGAPTPVGLETAEVG"
     gene            18698..19738
                     /locus_tag="PLW15_00095"
     CDS             18698..19738
                     /locus_tag="PLW15_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32830.1"
                     /translation="MNIGVPRYLVVGLAGLFSAYLLVLAVYTIDQPRHQAPIFVAMAL
                     FALATVVSLLPFGPKRMPIWMAAFNFAIVIAITLLVANELDFARPGGTGYASWYVAAS
                     GILLTITSTRGRPTFAWLGIGFLVVHTITIVGPAGPAGLLSLGIVGSASWVAVSHVMS
                     TALTKASKDAQRFALAEREATDWQAAQEAHVYERQFRLGQTSAMALAMLRIIQERDGD
                     LTDEQRQECLHLEGAIRDEIRGRKLLNDAVREEVMSARRRGATVTLLDEGGIDDLSEV
                     ELDRVLNRLAQAIHDTDADKVIARTVPEGSDVAVTVVGLRSSGDGQAVALGQDSLEDD
                     EVDLWLEIPRVV"
     gene            complement(20194..20403)
                     /locus_tag="PLW15_00100"
     CDS             complement(20194..20403)
                     /locus_tag="PLW15_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HOT32831.1"
                     /translation="MSEDSAFRIISKNATDEEIAAVSAVLQVALDELAAAQGPDDGPV
                     VSAWNRSQRGLRSPLTPGPGAWRSF"
     gene            complement(20400..22004)
                     /locus_tag="PLW15_00105"
     CDS             complement(20400..22004)
                     /locus_tag="PLW15_00105"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT32832.1"
                     /translation="MSEKTTPTPADLSTTAGRIADLKVRYHEAVTASGEAAIQKQHAK
                     GKKTARERIEQLLDHGSFVELDEFVRHRTHAFGMEKNRPYGDAVVTGLGTIHGRQVAV
                     YSQDFTIFGGSLGEVAGEKIIKVMDHALKTGVPIIGMLDSGGARIQEGVVALGKYGEI
                     FRRNTAASGVIPQISIVMGPAAGGAVYSPALTDFVIMVDKTSQMFVTGPDVIKTVTGE
                     DVGMEELGGALTHNKISGVAHYLASDEDDALDYARTLIGFLPDNNLAEQPVYESEVEL
                     EITDEDRKLNSIIPDSPNQPYDVKAIIEHIVDNGDFLETQPLYAPNIVVGFGRVEGRT
                     VGVIANQPQAMAGTLNIEAGEKAARFLRFCDAFSIPILTLVDVPGYLPGTDQEWTGVI
                     RRGAKLLYAYAEATVPLVTVITRKAYGGAYIVMGSKQMGADLNYAWPTAEIAVMGGQG
                     AVNILYRGEIKAAEEAGEDVAAVRTRLANEYTYNVASPFLAAERGELDGVIEPAATRV
                     AVIKALRALRTKRATLPPKKHGNIPL"
     gene            complement(22081..22881)
                     /locus_tag="PLW15_00110"
     CDS             complement(22081..22881)
                     /locus_tag="PLW15_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOT32833.1"
                     /translation="MTTDVYPEPVLPTATALRPRLANSIMQFATRINWAEAELRGLRA
                     IVRPGDSVIDIGAAHGMYTLPLAHLVGPTGRVESFEPHPRQQRTLRFLRGLVGVSQVS
                     VNAAAVGATTGERTMRLPLKLGFPIYGHAHITEGAAVHPTARVRQWNTMITSVDAWVK
                     AQGIDRVTFIKIDVEGFEPQVIEGAVATITNDHPSLLLEIEDRHLARYGKDGNSFAES
                     ILARWPEYGMYVWQDDRWVAADEVVLGIRNYLFATEDAFQTSRVTRSQ"
     gene            22955..23728
                     /locus_tag="PLW15_00115"
     CDS             22955..23728
                     /locus_tag="PLW15_00115"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT32834.1"
                     /translation="MHLPLSRAIAPHLHLLDEVGSTNAELVARAASGEAQDFTVVVTT
                     NQTAGRGRLDRVWVAPPGRTIAVSVLLSLPDLPLEKVGWMPLLAGLAMTRAVASLVDP
                     PRVSLKWPNDVQVDGLKVSGLLTELLVSPRAVVIGAGLNLTMTAEELPTPAATSLTLV
                     GADATDIVDRALAAYLGELRQLSDAYRGSGFDPEAGLRDQVVRVCGTIGQQVRVELPD
                     GSMLRGVAEGIDSVGRLLVRDDSGVQPVAAGDVTHLRYE"
     gene            23733..24200
                     /locus_tag="PLW15_00120"
     CDS             23733..24200
                     /locus_tag="PLW15_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT32835.1"
                     /translation="MTEAPVREVVVARLRPHVRVLFWPTVLLLAVCAAYGYFGGRYVD
                     GWPAIAIAAAAGVLVLVGWVYPLLAWLARNYTITTRRTVVRSGVFVRLRREVLHSRVR
                     SVTMRSTWLQSLFRSGDLEADLGGDQEVVLRDVPGAVLVQEVLHDLIEASRPD"
     gene            complement(24175..24654)
                     /locus_tag="PLW15_00125"
     CDS             complement(24175..24654)
                     /locus_tag="PLW15_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT32836.1"
                     /translation="MRALFGQFVRFGVVGLVGVVVDVGIFNLLRLTVLAPELIHEGPV
                     IAKVISTSIAIVTNWIGNRYWTFGSMRRSHVLREGLEFALVSVGGMGIALLCLWVSHY
                     LLGFTSALADNIATNVVGLALGTAFRFTFYRIWVFRPELEAKAAGEAESVSPVGSPQ"
     gene            24708..25862
                     /locus_tag="PLW15_00130"
     CDS             24708..25862
                     /locus_tag="PLW15_00130"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOT32837.1"
                     /translation="MPRISDNELGSTVSLTVGVIGGGQLARMMIPPAVELGIGLRVLA
                     ETEASSAGLAASAIGDYRDREAVLAFADTVDVVTFDHEHVPQVLLHELVDRGVRVHPG
                     PQALLYAQDKLLMRARLAELGLPVPDWAAVASVHDLTGFIDAHGGRAIVKTPRGGYDG
                     KGVRVVADARDVADWFDDDQPLLAEELVEFRRELSQVLARRPSGEIALWPVVESVQRE
                     GVCAEVIAPAPHSAGRVAQVAADIATSIAENLDVTGVLAVELFETTDDRLLVNELAMR
                     PHNSGHWSIDGSTTSQFEQHLRAVLDLPLGATGSRDPWSVMVNVLGGVADSAHDVRLA
                     AAMADQPDVKLHFYGKQARPGRKVGHVTAGGADLDDVVFRARAAAAFFED"
     gene            25906..26373
                     /gene="purE"
                     /locus_tag="PLW15_00135"
     CDS             25906..26373
                     /gene="purE"
                     /locus_tag="PLW15_00135"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT32838.1"
                     /translation="MGSDSDWSVMSAAAAVLTEFDIAHEVQVVSAHRTPAAMIAFGSA
                     AAGRGIRVIIAGAGGAAHLPGMLASVTTLPVIGVPVPLKTLDGLDSLLSIVQMPAGIP
                     VATVSIGGARNAGILAARILASGDTVLTAKLAEFAAALEAEVAQKNAALIASL"
     gene            26383..27756
                     /locus_tag="PLW15_00140"
     CDS             26383..27756
                     /locus_tag="PLW15_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT32839.1"
                     /translation="MTNASSPVRYPNTRSPEVMTRRAWVLVVLNILIPGSAQLLAGSR
                     RLGRFGVGSTFVLWGLIAIGALVYFLGRTAFITIATNSFALWVLQGVIIFYVVLWVVL
                     TLDTLRLVRFVNVEGNARIFVAVLSVAALVVVAGTGAYAVMIAGTTRSTVGEVFAGTT
                     IEPPVDGRYNILLLGGDAGPDRDGLRPDSISVVSVEASTGKATIIGVPRNMEDVPFVE
                     GSPLAAEYPDGYGVNDCQVDVCLINSIYTEVELYSPDLYPDAVANGSQPGIEAMRDAV
                     EGTLGITIQYYALIDMQGFADLIDALGGVTIDVAHRLPITDEVDENGQPYTDVPWIEP
                     GVQLMGGGTALAYARIRAGTTDYERMGRQRQVQEAILQQFDPANVLTKFQAVAQAGAQ
                     VVSTDIPQSMLGYFVELAGKSRELPVDTLDLVPDNGVDPEYPDFDYIRQLVAEALAPT
                     VEETPAP"
     gene            complement(27764..28891)
                     /locus_tag="PLW15_00145"
     CDS             complement(27764..28891)
                     /locus_tag="PLW15_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT32840.1"
                     /translation="MTTLRVIVDEMLSGAPGGVSRYAEELTRALISTAPKGVAIEGLV
                     SASTEPEYERITERLPGLATLYKSALQRRELSSAWQHGFTRLPGTGMVHAPSLFAPLA
                     KHDRLNDGDQIIVTVHDTLAWTAPQVLSPRRVSWHKAMLKRAERYADAIVVPSHTVAD
                     ELMTIAAVGERIRVIGGAPSSAIRIPADADALATELELPDRYVLAIGDIGAQNGLDQL
                     VAALAALDDDVALLLAGTEPASPALGSLADGAHVAPGRVRGLGALADPALSVALSRAA
                     VFAYPNLEEGFGLPVLDAFALGTPVIHSDAPALVELGAEAGLTVEREGEGYSERLAEA
                     IGSVLADSTLADRLRVEGIDRAGVFSWRGAADKVWQLHADL"
     gene            29171..30382
                     /locus_tag="PLW15_00150"
     CDS             29171..30382
                     /locus_tag="PLW15_00150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT32841.1"
                     /translation="MAKHVLLIAEARIATVQILELILHHLGQTTTLSVSSKLLSELEP
                     EDFTDDTYPLLVRSCSPEASSLVRALSSTGAPFGFYLDDNFWLLDPTTPLGRHYAAKP
                     TRRRLDSIVREATPVIASTPLLRDFLRPRARDVQQLDSFFDFALVPQVLPPRSSHARL
                     RVGFAASASRGDDLATVLADVCAALEEHPQLEFEVIGADPEALPDHDRIIAFDYLASY
                     AEYVAFQRTREWDIGIAPLGGATSNRYKTDNKYREYAALGIAGIYQESEPYSAVRDGV
                     TGLIVGRDRGWREAIDRYCAEPGLIDAVRAAARQDVEERLSLDVVAPQWGKFFSAAPA
                     IGDRPGRADAVRRALAPPSTPRALRAQRRRLLWAYGRTHLAERGFWFTLVRTVRYPFR
                     RRREKPFSLLR"
     gene            30395..31183
                     /locus_tag="PLW15_00155"
     CDS             30395..31183
                     /locus_tag="PLW15_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32842.1"
                     /translation="MTTATLDRQDTPSRIVRLSFFLGWQDVRLMYRRSVLGQFWITLS
                     MAITFLAIGSVFGLIFQSPVVEYLPFLGCGLVFWNFLSLIVNEGAVSFIVGDAFIRQL
                     PLPPIIFFLRSIWKTVFVLLHNAIALILLLLVFSPPPTPAILLVFPAVVLGGAGMAGL
                     ALALAVLATRFRDVPQILSALIQVLFYLTPIVWLPQSIPEQARGLILGINPFYHMIQI
                     MRQPLLGQLPTLQEWGIAGALAAVFIALGALVYARNYHRLAFWL"
     gene            31188..>31745
                     /locus_tag="PLW15_00160"
     CDS             31188..>31745
                     /locus_tag="PLW15_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT32843.1"
                     /translation="MTVHIHLDDVELDYPILSQSSRRLGRQLINVATGGQLDRSEQGR
                     ITVRALTDITLRLEVGDRVGLIGHNGSGKSTLLKLLGGIYRPTSGTVSRAGVVGSLLD
                     ISLGMSPDATGRENIFLRGALQGMSRSEIARLVPGIIEFSELGDFIELPMRTYSSGML
                     LRLAFSVATVLSPDILLMDEWLSVGD"
BASE COUNT         4921 a        10732 c        10728 g         5364 t
ORIGIN      
        1 ctcatcgtgt tcggccgcac gggaacggcg acctaccggc tctccaccga gagcgacctc
       61 atccagctgc ccgtgattcc tgccgacgtg caggcggtga gcatcggcat caccgtgctg
      121 atggttgcca tcaccgcggt ggcgttctgg ttcacctggc gcgctcgccc cacgcccctg
      181 tggctggtca gcatcttcgc ggtgctgttc gtgatcgggt tcctcacctg ggctgcggcc
      241 ggggcgctca tcccgatccc gggtctgctc gtcggggccg tcggcctgag tgttccgctg
      301 atcttcggtg cgctcggcgg cgtcatctcc gagcgcgtcg gtgtggtcaa cgtggcgatc
      361 gagggccagc tactcgccgg tgcgttcgtc tcggcggtcg tggcatccgt gatcaaggac
      421 tcgttcttcg gacctctgct cgggctgctg gccgctgtcg tggctggcgt gctcgtggcc
      481 ttcgtgctcg cagccttctc gatcaagtac ttcgtcgatc aggtgatcgt cggcgtcgtg
      541 ctcaacgtgc tcgtggtcgg tctcacgagc ttcctgttct ccaaggtgct cgcaccgaac
      601 gcggctctgc tcaacagccc cccgcggttc ccccgactgc cgattccgct gctcagtgaa
      661 ataccgatcc tcgggcccgt tctgttcagg cagacgatca tcgtctacat catgtatgtg
      721 gctgtggccg tcgtctggtt cgcgctgttc aagacccgat gggggctgcg cctccgttcg
      781 gtcggtgagc acccgcaggc cgccgacacc gtgggcatca acgtcaacag cacccggttc
      841 tggaatgtat cgctcgccgg tgccatcgcc ggtctcggtg gtgcgtactt cacgctcggt
      901 tcggtgggag ccttcaacaa ggagatgacc gcaggcgccg gcttcatcgc cctggccgcc
      961 gtcatcttcg ggcgctggga ccccatcaga gcaaccatgg ccgcgctcct gttcggcttc
     1021 gcatccaacc tgcagggcgt gctgagcgcg atcggctcgc cggtgccgag cgagttcatg
     1081 ctcatgcttc cgtacgtcgt cacgatcctg gcggtcgccg gcttcgtggg acacgtgcga
     1141 ggaccagcag cctcaggaaa gccgtacatc aaatcatgac cgactacctg cccaagaccg
     1201 acgccgactg ggatgccctg cgcggggtcg ccaaggaggc cctcagtcac gcctatgttc
     1261 cgtactccaa cttccctgtc ggggttgcgg cgatcgtcga cgacgggcgc gtgatctcgg
     1321 gtgccaacgt cgagaatgcg tcgtacggac tcaccctctg tgcggagtgc gcgctggtgt
     1381 cgtcgctgca catgaccggg ggtggaaagc tcgtcgcctt cacctgcgtc gacggccacg
     1441 gcaacgccct catgccctgc gggcgctgcc gccaactgct cttcgaacac tccgccgagg
     1501 gaatgttgct cgagaccgtc tccggcatca ggaccatcga cgaggtcatc cccgacgcgt
     1561 tcgggccgcg caccctcgag gcgtaccgcg ctacggactg acctcgacaa gttcgatcac
     1621 cgattggaac catcatggcc gtagaaccat tcgacgtcgt cgacctcatc cgcaccaagc
     1681 gggacaaggg cgtgctgacg actgagcaga tcacctggct cgtcgacgcg tacacccgtg
     1741 gctacgtcgc cgacgagcag atgtcggcca tgaccatggc gatcttcctc aacggcatgg
     1801 agcgcaccga gatccgcgac ctcaccctcg cgatgatcgc caccggggag accctctcct
     1861 tcgacggact cggcaagccg accaccgaca agcattcgac cggtggtgtc ggtgacaaga
     1921 tcaccctgcc gctcgcgccg ctcgtcgcgt cgttcggcgt cgctgtgccg cagctctccg
     1981 gccggggact cggccacacc ggcggcacgc tcgacaagct cgagtcgatc cccggatggc
     2041 gcgccaagct gtcgaacgac gagttctacg cgcagctgaa gtcggtgggt gcggtgatct
     2101 gcgcggcagg gtccgggctg gcgcccgctg acggcaagct ctacgccttg cgcgacatca
     2161 cgggcaccgt cgaagcgata cctctgattg cgtcatccat catgtcgaag aagatcgcgg
     2221 aggggaccgc ggccctcgtg ctcgacgtga agttcggctc gggcgcgttc atgcaggacc
     2281 aagcgcgcag tcgcgaactg gccgagacga tggtgcagtt gggcaaggat gccggcacga
     2341 agacggtcgc gttgctcacc aacatgaacg ttccgctggg actggcgatc ggcaacgcca
     2401 acgaggtgcg cgaatcggtg gaggtgctcg ccggtggcgg cccggccgat gtcgtcgagc
     2461 tcacgattgc cctcgcgcga gagatgctcg ccctcgccgg ccagcccgac gccgacgtcg
     2521 aggcggcgct gaaggatggc cgcgcgatgg acacctggcg cgccatgatc gcagcccaag
     2581 gcggcgaccc ggatgccgcg ctgccagcac cacgggagag ccaagtggtc acggccgacg
     2641 ccgacggggt tctcgccgcg caggctgcgc tgccgttcgg catcgccgcc tggcggctcg
     2701 gggccgggcg tgcccgcaag gaggacccgg tgcaccacgc tgcgggcatc gacctgcacg
     2761 ccaagccggg cgaccgcgtt accaagggcc agccgctctt caccatgcac actgacgagc
     2821 ccgcgcgatt tgagcgcgcg ctcgaagctc tcgagggtgc ctaccgcatc ggcgacacgg
     2881 ccgacgacgt gggcccgctg atcgccggtc gcgtcgactg acgctgcgcg cgcgcccgcg
     2941 ctccgcgctc gcccgcgctc gccgcgcccg atgaggtgcg gcaaacgcgc tctcgcgaag
     3001 gccaggagcg tgcgattgcc gcacctcatc ccggccagcc tcgctccctc aggcggcgtg
     3061 ctgttcgttt cagcagttcg cgggggtttg ctaggtcgtc ggcggacata cgcgtgtgat
     3121 gccagtgggc atcctcgagc agggggaccc gtcgcaggtc ctttcgccac tggcgggcat
     3181 ccgtgcggtg atggtctccc tcgtagtcga acgccatccg atactcaagc aactgaaggt
     3241 cgggcatggc gatgaaacgc ccgttcctgt cgtagacgtt ctggttgggc accggatcgg
     3301 ggaaccccgc tcgaatgaat cgcagccgga acgccgactc cggaatcgag tccgatcggt
     3361 cggtggtgag tggtaggaga gtctccactc gggacctgcc acggcggccc cgatggcggt
     3421 ctaggcactc gcgaaacgaa atccgggttg cgcgtgacgc agtcgaccgt cggcgccaga
     3481 agatgttgtc cagagctccg agcaggtgtt cgtcgacaag gaggggagcc aggtcgatca
     3541 cggtgcgctc cggcgaggta acggcgagcc cgttgatcgt ttcgatatcc gcgcggtgga
     3601 tctcgacacg atggcccgca actccccgac cttccggggc ccggcggtcg gaggggacag
     3661 agacgtggac cggcagcgga ttctcgagat ggatgggcag cgggatcgac cacagctggg
     3721 ccgccgtggc gtgcgagaag aaggcgtgcc cgggcatcct tgccgagtag gcccgggcga
     3781 ccgaggttag gtcgtccagg ctgccggcgg cggcgcgcac tccccagaac gggcgctcga
     3841 ggtcggctcc cgcgagccgg cgaggtccga tgcccagctg gagcgcagat gcgacggaga
     3901 atgcagcacc cagcgcgtcg gggagaggga tgcgcgcggt cacgccaccc acaatgcacg
     3961 accagcgccc cgcgacccga gagtcatcca cagggtgagg tgcggcaatc aaccgggaac
     4021 ggcgcgcctg tgcgtgcgtt tgccgcacct caccgagggc gtgactaggc ggggtcgccg
     4081 atccgctcca gcgcgtccac gacgagtgcc cagaacttgg cgtggtcgag gttgcgcgcg
     4141 acctgggtcg tgcagtcgtc gggagcgggc gcgcggaagt cggccacggt catcccgagg
     4201 gtgagagtgc cggtgagttc cacgtcgagc ggaacccgca cggcctgcat cacggtcggg
     4261 tcgatcacga aggccacggc gcaggggtcg tgcaccggcg ggtagtcgaa gccctgctcg
     4321 tctttgtagg tgtggccgaa gaactcgagc agctcgccca cgaacctcgc cggcgcggtg
     4381 tcgacggccg cgatcgccgc agcgacctcg ggcgtcgcga gcgcctcatg cgtcaggtcg
     4441 aggccgacca tggtgagcgg ccacgactcg ttgaagacga tgtgcgcggc ctcagggtcg
     4501 atcacgatgt tgaactcgct cgtcgccgac cagttgccga cgttgacccc gccgcccatc
     4561 agcaccacct gcttgacccg ggatgcgatg cgaggctctt tgcgcacggc cagcgcgatg
     4621 ttggtgaggg cccccgtcgg taccagggtg atctcgccgg gttcggacgc cataaccgtg
     4681 tcgatgatga agtccaccgc gtgccgggga tcgagcgaca acgtgggctc gggcagcacg
     4741 ggaccatcga gccctgactc cccgtggatg gtgtcagcga actcgaccgg gcgcacgagc
     4801 ggccgatgcg aaccccgcgc gaacggcacc ccggtgattc ccgcgacacg agcgaccgcc
     4861 agcgcattgc gcgtgacttt ctcgatcgac tggttgccca tcaccgtggt gacgccgacc
     4921 aggtcgatgt cggggttgcc ccaggcgagc agcatcgcga tcgcgtcgtc gtggccgggg
     4981 tcgcagtcga ggatgatctt cgtcgccata cctcgatcct agcgtgattt gcatatatga
     5041 aagccaccct catatgtgta gagtgctcgc atgaccgacc acagtgcctc caccaccgat
     5101 ctcagtgccc gcggcgccgc ccgcgtcgaa tggatcagat cccggatgcc gctgctcgcg
     5161 agagcccgcg ccgagttcgc cgagcggctg cccttcgccg gccaccgcat cggcatgagc
     5221 ctgcacctcg agcccaagac cgctgtgctg ctggagactc tggctgccgg gggcgccgag
     5281 gtcgtggcga ccggcaacca cggttccacc caggacgaca tcgtggcctt cctccgtggt
     5341 caggggatga ccgtgttcgg ctcgcgcgac gacgacctcg accagcacca ccggaacgtg
     5401 gcatccgtcg ccgaggcgaa gccgacgatc ctgctcgaca acggtgccga cctcgcggcg
     5461 ctcgcggcgg cggacccgac cggcatcatc ggcggcacgg aggagaccac ctcgggcggc
     5521 ctccggctgc gcggcgagct cgcggggcgc atccccttcc cgatgatcgt catcaacgac
     5581 tccctgctga aggcgatcgg cgagaacaag cacgccgtcg gccagtccgt ggtcgagtcg
     5641 ttcatgcgca tcaccaacct gatggtcaac tccaagcggt tcgtcgtctt cggctacggc
     5701 tggtgcggtc ggggcgtggc gcagtacctc cgggcgctcg gcggcaaggt cgcggtcgtg
     5761 gaggtcgacg agatcaaggc gttcgaggcg gcgatggacg gcttccgggt cgacaccgcc
     5821 gacgggctcg ccgagtgggg ccaggtgttc atcacggcca ccgggcatcc gggagtcctc
     5881 acggcgccgt tcttcgagaa ggtcgccgac ggagccgtgc tggccaactg cgggcacttc
     5941 ccctgggaga tcgacgttcc cgccctgcgc gcctacgcga tcggcagcac ggtgctcgac
     6001 gacgcgatcg agcgtctcgg cctgccgggc gggcgccacg tcatcctcat cgccgacgga
     6061 cgcatgttca acctcgccgg ccgcgaaccg aagggcaact cgctcgagtc gatggacctc
     6121 ggcttcctgc tccagtcgct ctctctcgag cgggtcgcca ccgcagccga caccctcacc
     6181 ctgggcgctc agccggttcc cgaggacatc gaccgcgaga tcgcccgacg gatgctcgcc
     6241 gcactgtccg ccgctcgctg atgccgcgcg acccggaggt gcccccagct cccgacaccg
     6301 cgtacgccgt gccggccctc gagaagggtc tcgacatcct cgagctgctc gcggacaccg
     6361 ccggcggcct cggccagacc gagatcgccg aggcgaccgg ccgcagcgtc gggcagatct
     6421 tcagggtgct cggggtgctc gagcgccgcg gctatgtcgt gcgcgaccgg cagagcgggc
     6481 tgtactcctt ctcgatgatg accttcgacc tcgcgcaccg gcatccgccg ctgcgggggc
     6541 tcatcatgct ggcaacgact cccatgcgcg agctgtcgga tcgggtgcgc cagtcgtgca
     6601 acctcagcgt gctcgacgcg gcgcgagtgc gcgtgatcgc gcaggtggag agccccgcag
     6661 acttcgggta ccgggtgcgg gtgggcgccg tcttcccgct ggagaccgcc acgggtgagg
     6721 tgctggaggg cgccgccgcc ctcacgcggg ccgacccgct gcaggcgggc atcaccgacg
     6781 tggtggcccc gatcgtcgac gcgcgcgggg ttacgcgggc cgcgctgacg gtgccgtacg
     6841 tcgcgaccac cttctcgggc atcccggtcg acgaggttgt ggccgcagcg gaggacaccg
     6901 cgtcggagat ctcacgactc ttacagggtg aaccccggta gattggggca catgaacgcc
     6961 gctgttaccg agttcctcat gcccgggggt ggagcgagta tcacggcgct gcccaaggtg
     7021 tccctccacg accacctcga tggcgggctc cgtccgcaga cgatcatcga gctggcggat
     7081 gcgatcggca tggccgttcc cgcatcggat gccgcggctc tcgccacctg gttcgccgag
     7141 aagtccgact cgggttccct ggtggagtac ctcaagacct tcgatatcac caccggagtg
     7201 atgcagaccc acgagggatt ggcgcgcgtg gcccgcgagt tcgtgctcga tctgcacgcc
     7261 gacggcgtca tctacgggga gatccggtgg gcacccgagc agcacctgag ccggggactc
     7321 accctcgacc agactgtcga agccgtgcag gagggcctgg aggccggcgc tgaggaggtc
     7381 cgtcacgcgg gtggcagcat ccgggtcggg cagttggtca gcgcgatgcg ccatgccgac
     7441 cgcggcctcg agatcgccga gctcgccgtt cggcaccgca acaacggcgt cgtcggattc
     7501 gatattgcgg gcgccgaggc gggcttcccg gccggcaacc tacgcgatgc gttcgactac
     7561 ctcgcccacc acttcttccc cgcaacagtc cacgcgggcg aggccgacgg tctcgactcg
     7621 attcgcggcg ccctgttcga cggccgtgct ctgcgcctcg gccacggagt gcgcctcgcc
     7681 gaggatgtgc acaccgcccg cgaggacgac gagaacacct acgtcacact cggcccggtc
     7741 gcccagtggg tgaaggaccg cgagatcgca ctcgagctgt caccgagctc caacctccag
     7801 accggcgcga tcgagcagtg gggcgacgag ctcatcgatc accccttcga cctgctgtac
     7861 cagctcggat tccgcgtgac ggtcaacacc gacaaccgac tcatgagcgc cacctcgctc
     7921 agccgcgagc tcgccctgct cgcggagacc ttcgactacg acctcaccga cctggcggtg
     7981 ttccagctca acgctgcggc atcggcgttc ctccccttgg aggaccgcga ggatctggcc
     8041 gaccgcatca ccgccggctt cgaagaggcc taacggatgt cgctgccccc actccccgcc
     8101 agcgccatcg tcatcggggc tcacgccgcc gattggcgag ccgcggtcac cctcgcgggc
     8161 gacgcgctcg ccgcctcagg cgcggcgaag ccggagtact cgcgcgagat gatccgcatg
     8221 atcgaggagc acggtcccta tgtggtgatc gcgccaggtc ttgctcttgc ccacgctcgg
     8281 cccggccccg aggtgctcgc cgacggactc tcggtcgtga ccctggccga gccggtgtcg
     8341 ttcggccacc cgtacaacga tccggtgagc gtcatcctgg ggctcgcgat cctcaccgcc
     8401 gaccgtcatc ttgccgtggt cgccgctgtc gccaacatct tcaacgactc gacggcgatc
     8461 gccgatctgg cggccgccac gaccgccgcc gacgtgcagc ggatcatgca ggttcccgcg
     8521 tgaagatcgt gaccatctgc ggtgcgggaa tcggcagctc gggcatcctg aaggtcaacg
     8581 cggagcgagt gctgcgcaag ctcgacctcg aggcgacggt cgtcgccgcc gacatctcca
     8641 cggtcaagga tgtcgcctca gacgcgcagg tcatcctcac ctctcaggag ttcgtggatg
     8701 cgatcggctc gaccttcgcc gacgtgatcg tgatccagaa ctacttcgac acggaggagc
     8761 tgaccgccaa gctcaggtcg gccctcgaat aggcttcctc agcccgcgag caactcgcgc
     8821 atgccggcgt cgagttcggc gatgacggcg gctgccgcac tctggcgcgc cgtggcatcc
     8881 ccgtcggtgc tcgacgcatc gaggtagacc ttgagcttgg gctccgtacc gctggggcgc
     8941 acgatcacgc gtgcaccgtt gaccaggtgg atgcgcagga tgtcgcccgg cgggaactcg
     9001 ccgaagccat cgatgaagtc gtcgatgcgg tcgacctcga gggtgccgat ccgttccggt
     9061 agatgcgcgc gcagctgggc catcatcgcg ccgatgcgcg acaggtcgtc gacgcgcagc
     9121 gagatctggc ccgaggcgaa cgcaccgaac ttggccgcga agtcagcgag gtggtcgacg
     9181 acggtcttgc cctgcgactt cagctccgac accagggaga ggaagtcgac ggcggctgag
     9241 atgccgtcct tgtcgcgcac cttgtcggga tcgacgaggt agcccaacgc ctcctcgtag
     9301 ccgaaggcga gcccgccgac gcgggagatc cacttgaagc ccgtcagggt gtcgcggtag
     9361 tcgaggttgt agctgcgagc cacctcccgc agtgccggcg acgagacgat ggatgccgcg
     9421 agcacctgat cgggattgca ccgctgcgcg gctcgccacc ccagcagata ccccacctcg
     9481 ttgcccgaga gcgcgcgcca gccgctcgcg gtcggaatgg ccaccgcgag ccggtcggcg
     9541 tcgggatcgt tgacgatggc gacgtcagcg ccgacctccg ccgccttcgc gagcgtgaga
     9601 tccatggcgc cgggttcttc ggggttgggg aacgcgaccg tggggaacgc cgcatccggc
     9661 tcgatctgct cggtgacaag ggccggggcg gcgaatccgg cagcggcgaa gaccttgccg
     9721 gccgtctccc agcccactcc gtgcatcgcc gagtagacga acgagatcgg cgcgggcgtc
     9781 gagcccagct cggccgtgcg gcgcacgtac tcctcgagga tcgactcgtc ggccaccacg
     9841 aagtcggtcg agcgcgggag gtcggtgatc gagcccgtcg agatcagagc gatgtctgag
     9901 gcgatctccg catcggcggg cggcacgatc tgcgagccgt ggtcgaaatc gccgaggtag
     9961 accttgtagc cgttgtcgcg ggccgggttg tggctcgccg tcaccatgat gcccgcgctc
    10021 gcctcgaggt gccgcaccgc gaacgccagc accggggtcg gcaggtggcg cggcagcaag
    10081 gtggtgcgca cgccggcgcc ggccatcacg acggccgagt cacgcgcgaa gacctcggag
    10141 ttggtgcgcc cgtcgtagcc gatcacgacg ctcgggctgg gggacttcga caggaggtag
    10201 gcggcgaggc ccgcggccgc ctgagtgacg agcactcggt tcatgcgggc ggggcctgcg
    10261 cccagctcgc cgcgcagtcc cgccgtgccg aactgcagcc ggctcgcaaa gcgctcggcc
    10321 agtggctcgg tgtccccggc ctcggcggag gagatcagtg cgtcgagttc ggcgcgggtg
    10381 gtgtcatccg gatcctggtc gcgccaggcc cgggcatcgt cgatcaggct cacggctaca
    10441 gctccccgac gatcgtggcg agcagcgcgc tgatccgcga ctccgcctcg cgcccggctt
    10501 cgatcacttc ggcgtggctc aagggtgtct tctggatgcc cgcagcaagg ttcgtgatga
    10561 gcgacatccc gaggatctcc atgccggcct gccgtgcggc gatcgcctcg agcgcggtgg
    10621 acatgcccac gatgtggccg ccgatgatct tcgccatctg cacctcggcg ggggtctcgt
    10681 agtgcggccc gcggaactgg cagtacacgc cttcatcgag gctcgagtcg accgagcgag
    10741 cgaggtcgcg caggcgcttc gagtacaggt cggtgaggtc gatgaaggtg gcgccctcga
    10801 gcggcgagtc ggccgtgagg ttgatgtggt cgctgatgag cacgggtgtt ccgggcttcc
    10861 aggtctcccg gatgcccccg gccccgttcg tgaggatcat ggtcgtcgcc cccgtggcgg
    10921 cggccgttcg caccgagtgg acaacacggc gcacgccgtg ctgctcgtag tagtgagtgc
    10981 gtgccccgat caccagcgca cgctttccgc tgggcagcag caccgagcgc agggtaccga
    11041 cgtggccctc gagtgccggc ttggagaagc cggtgatctc gaaggcggga atcgtggcgg
    11101 tggtctcacc gatcaggtcg gcggccttcg cccagccgct cccgagcgtc agggcgatat
    11161 cgtggcgctc gactcccgtg cgttccgcga tttccgcggc cgccactgcg gcgatggcga
    11221 aggggtcggc ggtggggtca tcgaggggat tcgtgggggt cttcagcgac atcccaccac
    11281 tctacttctg agacaattgc ctcatggcct acaaattcga gcggacccag cgcatcgcag
    11341 tactcggtgg cggtcccggc ggatacgagg cggcgctcgc cgcggcacag ctcggtgccg
    11401 aggtcaccct ggtcgagcgc gagggcgtcg gcggctcggc ggtgctcacc gacgtggtgc
    11461 catccaagac tctgatcgcg accgccgagg cgaccaacag catcggtgag gcggccgacc
    11521 tcggcgttca gttcttcgtg cgctccgaat ccggcagggc ggttcgcccg gacgtgacgg
    11581 tcaacctcgc ggcggtcaac gcccgactgc tgcggctggc tcgccagcag tcggaggaca
    11641 tgaagtcgca gctcatcaag gcgggcgtgc gcatcatcca gggcgaaggt cgacttgacg
    11701 gcccgcagcg tctcgtggtc tcaacgggca agggcaagaa gggcaccgac ttcgacgaga
    11761 tcgacgccga caccttggtc gtctccgtgg gcgcccgacc gcggatgctc gacagcgcta
    11821 tgccggacgg cgagcgcatc ctcacctgga cccagctgta cacgctcgac caggttcccg
    11881 agcacctcat cgtggtgggt tcgggtgtga ccggcgccga gttcgcctcc gcctacaccg
    11941 cgctcggctc gaaggtcacc ctgatctcct cccgcgagca ggtgctgccc ggtgaggatg
    12001 ccgacgccgc gcgcgtcatc gaggacgtct tcaagcgcaa cggcatgacc gtgctctcga
    12061 agtcccgcgc cgactccgtg aaggcgacca agaccggcgt gatcgcgacc ctcgccgacg
    12121 gccgggtcgt cgagggcacg cactgcctga tggccgtcgg atcgcttccg aacactgagg
    12181 acatcggtct gaaggaggcg ggggtgcagc tcaccgactc cgggcacatc cgggtcaacc
    12241 gggtcgcgcg cacgtcgatg ccgtccatct acgcggccgg cgactgctct gacttcctgc
    12301 cgctggcatc cgtcgcctcg atgcagggtc gcaccgcggt gtaccacgcc atgggcgatg
    12361 ccgtgtatcc gactgagctg cgcaacgtca cctcgaacat cttcacccag ccggagatcg
    12421 ccaccgtcgg ctggtcgcag aagcagatcg aggaaggcct caccaccggc atcatctaca
    12481 agctgccgct ggcgtccaac ccgcgggcga agatgatggg catcaaagac ggcttcgtga
    12541 agctgttcac gagtacccgc tccggaacgg tgatcggcgg ggtgatcgtc gccccgcacg
    12601 catccgagct gatcctgccc gtgtcgctgg cagtcgagca tcggctcacg gtcgaccagt
    12661 tggcgcgcgc gttcccggtc tacccgtcgc tgaccggcag catcacggat gctgcgcggg
    12721 cgatgcacat cgtcaactag cgaccgcccg tcgctacggc ctctgtcgca cgatccctgg
    12781 ctgcggtcac gcgccagccg tgtgatctcg caagtgctcc cgttgcgcag gagacgcgag
    12841 gattctttgg gtttcctgcc caactggagt gcggagggtg agagtcccgc gagcgggttg
    12901 cctcactcct ggaggcaacc tttggcaggg cggctcgcgt ccctcagtcg gcgatgctca
    12961 gcagcaggtg accggatgac acggtcgcgc ccacggtcgc gttgatgccg gtgaccgttc
    13021 cgtccttgtg ggcggtgagc ggctgctcca tcttcatcgc ctcgagcacg aggatcaggt
    13081 cgcccttgac gaccttgtcg ccctcggcga cggcgatctt caccacggtc gcctgcatgg
    13141 gcgccttgac ggtgtcaccg gtgacggtgc tgacggatgc cgcggcactg cgccgaggcg
    13201 cagcagccag cgccgttccg ccgcccgccg tgggcaccag cttcgtgggc agcgacacct
    13261 ccacccgctt gccgccgacc tcgacgacca cgttgtggcg ggcgggcgga gccgacccgt
    13321 cgggcagcga tccgctccac ggctcgatgt cgttgtcgaa ctcggtctcg atccagcggg
    13381 tgtacacgcg gaacgggctg tcggtgaagg cggggtcgcg cacgatcttg cggtggaacg
    13441 ggagcacggt cggaaggccg gccacctcga attcgtcgag ggccctgcgg gagcgctcga
    13501 gggcatccgc ccggctggat ccagtgacga tgagcttggc cagaagggag tcgaacgcgc
    13561 ccgagatgac gtcgcccgtg gtgacaccgc tgtcgacgcg cacgccgggg ccgccaggga
    13621 agcgcagggc gttgaccggc ccgggggagg gcaggaagtt cttgcccggg tcttcgccgt
    13681 tgatgcggaa ctcgaacgag tgcccctgcg gcttcgggtc gtcgtagtcg atgacgccgc
    13741 cctcggcgat gcggaactgc tcgcgcacga ggtcgattcc ggtgacctcc tcggagaccg
    13801 ggtgctcgac ctgcaggcgg gtgttgacct cgaggaagga gatcgtgccg tccttggcga
    13861 ccaggaactc acaggtgccg gcgccgacgt agccgacctc cttcaggatc gccttcgatg
    13921 acgtgtagag ggccgcattc tgctcgtcgg tgaggaacgg tgccggcgcc tcctccacga
    13981 gcttctggtg gcgacgctgc agcgagcagt cgcgggtgga gatgacgacg acgttgccgt
    14041 ggctgtcggc caggcactgg gtctcgacgt gacgcggctg gtcgaggtac ttctccacga
    14101 agcattcccc acgaccgaag gccgccacag cctcgcgggt cgcggagtcg aacagctcgg
    14161 ggatctcctc gcgggtgcgg gcgaccttca ggccgcgacc gccgccgccg aacgccgcct
    14221 tgatcgcgac cggtaggccg tgcaggtcga cgaagtcgag cacctcggcg gcgcccgaca
    14281 cggggttgag ggttccgggg gcgagcgggg cgccgacctt ctcggcgatg tggcgagcac
    14341 tgaccttgtc gccgagcttc tcgatcgcgt caggcgacgg accgatccag gtcagcccgg
    14401 cgtcgatgac ggcgcgggcg aagtcggcgt tctccgcgag gaaaccgtag cccgggtgca
    14461 ccgcgtcggc tcccgagcga cgggcgacgg agagcagctt gtcgatcacg aggtaggtgt
    14521 cggcactcgt ggtgccgtcg agcgagtacg cctcgtcggc gagcttgacg tggcgggcat
    14581 cccgatcctg gtcggcgtag accgcgacgg aggcgatgcc gctgtctttg gcggcgcgga
    14641 tgacacgcac cgcgatctca ccccggttgg cgatcagaac cttcgtgatg ggagccatag
    14701 tcacctagcc tattggggcg gagcggcacg cctttgggcg atgccgacaa aaacgggtcg
    14761 agagtcctgt gtgaggctac aagttcgcgc ggttccacag cgtgtgccac gggatgccca
    14821 gctcgacgac cagctgccgc agcagtgcga cggagattcc caccaccgcg tgcgggtcgc
    14881 cgtcgacacc ggtgatgaag gccgagccga ggctgtcgat cgtgaaggcg cccgccaccc
    14941 gcaggggttc cccggtggcg acgtaggcat cgatctcctc gtcgccgagg tcggcgaagc
    15001 tgacaccggc cgacgacacc ccgcccgcgc cgccggagtc cactccaccg cgatggtcga
    15061 tgatccacgt gcccgagtgc agcgtgcccg agcgcccccg catgctgcgc cagcgctcga
    15121 tggcgacctc cggatgatgc ggcttgccga acacggcgcc gtcgaactcg aacagcgagt
    15181 cgccgcccac gatgatgccg tcgatcggtt cgccgcccac gagagtcccc acgatcgctt
    15241 cggccttggc gcgcgcgagc acctgcaccg cctcgtgcac gggcagcggc ccggccgccg
    15301 ccgcgaccgc atcctcgtca accgccgggt cgagcacgat cggctcgatg cccgcggcgc
    15361 ggagggtcgc caaccgtgcg ggggaggtcg atgcgaggta gagacgcact gagggctcct
    15421 aggctggggg gatgggtgac tggctgggca ggacgatcga actcgacgtt accaacatcg
    15481 cgcacggtgg ggtctcggtc gcgcggcacg acggccgtgt cgtgttcgtc gccgacgcga
    15541 tcccggggga gcgagtgctc gcgcgggtgt cgagcgactc caagaagagc ttctggcgcg
    15601 ccgagaccgt cgaggtgctc gaggccagcc cgcaccgcca gccccacgtg tgggatgccg
    15661 cggctctcga ccgcgatccg gtcgaccgtg cgggcggcgc cgagttcggg cacatcgaac
    15721 tcgcccacca gcgcgagctc aagcggcagg tgatcaccga ctcgctcgcc cgcatggcgc
    15781 gccttgagcg agacgtcact gtcgaggccg tgcccggcga cgacgaacgc gccggcaccg
    15841 gatggcgcac ccgggtgcgc ctgcacgtcg acgaccaggg gcggcccggc ccgttcgccg
    15901 cccggtcaca ccgggtgatc cctgtcacga gcctgccgct ggcgaccgcc gagctcgagg
    15961 catcagcgcc gctcggggag cggttcgacg gctacgagag cgttgacgtg ctcaacccgt
    16021 cgacgggggg cgtgcgtctg atcgtcggaa atcagaagcc cagcgtgatc accgagcggg
    16081 tgggcgagcg cgagttccgc ctcgccgaca gcgggttctg gcaggtgcac cgcggggctg
    16141 cggccaccct gaccgaggcg gttcagtcgg ccatcgatcc ggagctgttc gatccgcagg
    16201 ccgcgaacct cgacctgtac ggcggcgtcg ggctccttgc cgccgccgtc gccgatcgct
    16261 tcggcccgtc cacgcgcatc acgagcgtgg agagcgacgc gaccgcgacc gacttcgccg
    16321 ccgagaacct gtcggaatgg ctcggagcgc gagccgagac gggtcgcgtc gagcgctggg
    16381 tggccgcgct cgcggcatcc gctggcccct cggagcgcga gcgactcgcc agggcgactg
    16441 tcgttctcga ccctccccgc tcgggcgccg gacgagatgt cgtcgaggcg gtcgcgcggc
    16501 tgaagcccgc ccaggtggtc tacgtcgcgt gcgatccggt ggcctttgcc agggataccg
    16561 ccctgtttgc cgatctcggg taccacctcg gggggttgcg ggcgttcgac ctgttcccgc
    16621 acactcacca tgttgaagcc gtgggaacct tcactcgcac ctgaacgggc ggaatgttgg
    16681 ccccgcgata gtgctctgac gacagtacga tggggtacac ccggcagaat ggacgtgcat
    16741 ggcccaggca tctactacgg aagtcggcag cgatatcgcc gcaccgcagg tgcgcgttgc
    16801 cgtcgtcgac gaccacgagt cagtgcgact cggtctcaag gctgccttcc tcgcggaggg
    16861 ctacgatttc gtgctcgccg cacccaccgt ctccgaactc gttgaaggtc tcgacggccg
    16921 cgaggtcgac gtcgtcgtgc tcgacctctc gctcggcgat gggtccaccg tcaccgacaa
    16981 cgtcaagcgg gtgcaggcca ccggcgcggc cgtactcgtg cacagcatcg ccgaccgcgt
    17041 ggcctacgtg cgcgaggcgc tcgcggccgg tgcggcaggc gtcatcccca agtcgtcggc
    17101 gaccaagacc gttctcgccg ctgccgcgac agtcgcgcgc ggcgaggtgc tcaacaacct
    17161 cgagtgggcg acggcaatcg acgccgaccg cgacttcgcc aaagcccagc tgggtcggcg
    17221 cgagcgcgag atcctgcacc tctacgcctc gggactgccg ctcaagctcg ctgcccagca
    17281 gctgggcatc ggatactcga ccgccaggga gtacctggac cgcatccgcg tcaagtacgt
    17341 ggaggtcggc cgaccggcgc ccaccaaggt cgatctgctg aggcgcgcgg tcgaggacgg
    17401 catcctgccc ggtctcgatc cggatggcgg agatgcccgc tagcgcgaca tcggtcgccg
    17461 ctccgccgca gtccaagcag ccgcggaatc cgatcagccg caagcaggtc gagaacgtct
    17521 tcgcgcgctc ggttgccgtc ttcggtctcg tgttcggggc ccagaccatc ccgtggatgc
    17581 tcggccagct cgacgaggcg tatccgctgt ggctgtggat cgtcgtcccg tcgctcttcg
    17641 gcatgctcgt ggtcgttctc atcacgtcgt tcgcgaaggt gtgggtcaag ccggctcagg
    17701 gtctgttcgc gatcctctac ctcgcggcgc tcatcacgtg gcccttgacc gtgctgccgg
    17761 gggcggagat cttctcgggc atccactggc tcaactacct catcaccgtg gcgacggcga
    17821 tggcggccat cgcgttcagg ccgctcattg caaccatcta cctgtttctg tgcccgacca
    17881 tctacgtgct cttccgggcc acgccgatcg gcggaggggc acccccgttg ctggcgctgt
    17941 tcgaaggcat ctacgcggtg atcctgggtg gcgcgatcat catcatcctg acgatgctgc
    18001 gccaggccgc cagctccgtt gacaacgccc aggccacggc gctcgaccgg tacgcgcacg
    18061 cggtgcgcca gcacgccacc gaggtggagc gcgtgcaggt cgactcgatc gtgcacgaca
    18121 gcgttctgac gaccctcatc tccgccgcac gcgcgttcac cccgcaggcc cagcacctgg
    18181 ccgcgacgat ggccacgaac acgatcggct atcttcggga tgccgcggcc gccagcccag
    18241 acgacggatc catggtgcgg ctcacgtcgt tggccgaacg catcacggag accgtcaacg
    18301 gctactcggg tgtcttcgaa ctcagggcgc gcagcgtagg aacccgctcg atcccggctc
    18361 aggccgctga ggcgctctac tccgctgctg ctcaggcgat ggtcaacagt tcgcggcacg
    18421 cgggcgacac cggcgtgacc cggtgggtcg cgttccgcag cgttgcgccc ggcggcatcg
    18481 agatcatcgt cggtgatacc gggcgcggat tcagcgtggc ggacatcccc gaggagcgca
    18541 ttggcgtgcg catctcgatc atcgagcgtc tcgcgaacgc gggcggtcgt gcggtcatcc
    18601 agtccgcacc tgacgaaggc acactcgtga gcctccgttg gccgaacccc gccggtgctc
    18661 ccactccggt cggcctcgag acagccgagg tcggctgatg aacatcggcg tgccccgcta
    18721 cctcgtcgtc ggcctggccg gactcttctc cgcgtacctg ctcgtgctcg cggtctacac
    18781 gatcgaccaa ccgaggcatc aggctccgat cttcgtggcg atggccctct tcgcgctggc
    18841 gaccgtcgtc tcgctgctcc cgttcggccc caagcgcatg ccgatctgga tggcggcctt
    18901 caacttcgcg atcgtcattg ccatcacgct gctggtggcc aacgaacttg acttcgcccg
    18961 gcccggaggc acgggctacg cgagctggta tgtcgcggcc agcggcatcc tgctgaccat
    19021 cacctccacc cgtggtcgcc cgaccttcgc ctggctcggt atcggattcc tcgtcgtgca
    19081 cacgatcacc atcgtgggtc ccgctggacc cgcaggcctc ctcagcctgg gaatcgtggg
    19141 cagcgcctcg tgggtcgccg tgtcgcacgt catgagcacc gcgctgacca aggcctccaa
    19201 ggatgcccaa cggttcgcgc tcgccgagcg cgaggcaaca gactggcagg ccgcgcagga
    19261 ggcccacgtc tacgagcgtc agttccggct gggtcagacc agcgccatgg cactggccat
    19321 gctcaggatc atccaggagc gtgacggaga tctgaccgac gagcagcgcc aggagtgcct
    19381 gcacctcgag ggcgcgatcc gtgacgagat ccgcggccgg aagcttctca acgacgccgt
    19441 acgcgaagag gtcatgagcg cccgacgccg cggtgcgacg gtcacccttc tcgacgaggg
    19501 cggcatcgac gacctctccg aggtggagct ggatcgggtg ctcaaccgtc tcgcccaggc
    19561 catccacgac accgacgccg acaaggtgat cgctcgaaca gtgcccgaag gctcggatgt
    19621 cgccgtcacc gtcgtgggac tgcgaagctc aggggatggc caggctgtag ccctcggaca
    19681 ggactcgctg gaggacgacg aggtcgacct gtggctggag attccgcggg tcgtgtagat
    19741 tccggggcgg tgacgcacga agggccggga gcgtggctcc cggcccttct gcacgtttcc
    19801 gggtcagggc gataacccga atatcgccct gacccgcccc caagcgattg gcccgcttac
    19861 cctagtcggc caatcgctgg tggtgatctc aatgagattc acctagcacc atcaagtttg
    19921 gtcgcagggc gggatagaga acagtcgtct ctttggggga ctagaggggg acaattcggg
    19981 gttcaatgaa ggaaattgtg cccgaaatca tgggcttttc acggaagccg gtttcgaaat
    20041 caggggtaca aacgtcgctt ttcggcggaa tcagcggcct gcagagggta gaacggtctg
    20101 tatggcccgt cagtgcttcg gatttcgttg ttccaagcca ctcgtaaccc ggtttccgca
    20161 ccaagtgacc cccctctggg ggtgattacg gcgtcagaac gaacgccacg cccccgggcc
    20221 gggcgtgagt gggctgcgca ggccgcgctg gctgcgattc cacgccgaga cgacggggcc
    20281 gtcatccggg ccctgggcgg ctgcgagctc gtcgagggcg acctggagca cggcgctgac
    20341 cgcagcgatc tcctcgtcgg tcgcgttctt ggagatgatc ctgaaggccg agtcctcact
    20401 cacagcggga tgttcccgtg cttcttcggg ggaagggtcg cgcgcttcgt gcgcagggct
    20461 cggagcgcct tgatcacggc cacgcgggtt gccgccggct cgatgacgcc gtcgagctcg
    20521 ccgcgctccg ccgccaggaa cggggatgcc acgttgtagg tgtactcgtt ggcgagccgg
    20581 gtgcgcaccg ccgcgacatc ctcgcccgcc tcctctgcag ccttgatctc gccgcggtag
    20641 aggatgttga cggcgccttg gccacccatc acggcgatct cggccgtcgg ccaggcgtag
    20701 ttgaggtcgg cgcccatctg cttggaaccc atgacgatgt aggcaccgcc gtacgccttg
    20761 cgcgtgatca cggtgaccaa cggcaccgtg gcttccgcgt aggcgtagag cagcttggcc
    20821 ccacggcgga tgacacccgt ccactcctgg tcggtgcccg gcagataccc cggcacatcc
    20881 acgagcgtca ggatcgggat gctgaacgca tcgcagaagc gcaggaagcg cgccgccttc
    20941 tcgccggcct cgatgttgag ggtgcctgcc atggcctgcg gttggttggc gatgacgccc
    21001 accgtgcggc cctcgacccg tccgaagccc acgacgatgt tgggcgcgta gagcggctgg
    21061 gtctcgagga agtcgccgtt gtcgacgatg tgctcgatga tggccttcac gtcgtagggc
    21121 tgattggggc tgtcggggat gatcgagttg agcttgcggt cctcgtcggt gatctcgagc
    21181 tccacctcgc tctcgtagac cggctgctcg gcgaggttgt tgtcgggcag gaacccgatg
    21241 agcgtgcgcg cgtagtcgag cgcgtcatcc tcgtcgctgg cgaggtagtg cgcaacgccg
    21301 gagatcttgt tgtgggtgag ggcgccaccc agctcctcca tgccgacgtc ctcgccggtg
    21361 acggtcttga tcacgtcggg tccggtcacg aacatctggc tggtcttgtc gaccatgatg
    21421 acgaagtcag tgagagccgg cgagtacacg gcgccaccgg ccgctgggcc catgacgatc
    21481 gagatctgcg ggatgactcc ggatgccgcg gtgttgcggc ggaagatctc gccgtacttg
    21541 ccgagggcga ccacgccctc ctggatgcgg gcgccaccgg agtcgagcat gccgatgatc
    21601 ggcacgccgg tcttgagggc gtgatccatc actttgatga tcttctcgcc ggccacctcg
    21661 ccgagcgagc cgccgaagat ggtgaagtcc tgcgagtaga ccgcgacctg gcggccgtgg
    21721 atggtgccga ggccggtgac gaccgcgtca ccgtacgggc ggttcttctc catgccgaac
    21781 gcgtgggtgc ggtggcgcac gaactcgtcg agctcgacga acgagccgtg gtcgagcagc
    21841 tgctcgatgc gctcgcgggc ggtcttcttg cccttcgcat gctgcttctg gatggcggcc
    21901 tcgccgctgg cggtcacggc ctcgtggtag cgcaccttga ggtcggcgat gcgcccggcc
    21961 gtcgtggaca ggtcggctgg ggtcggggtc gtcttctcgc tcacgctcac actctaccga
    22021 gagcgcacga accggctttg gtgacgagct acagcagtgt cgcggttttc ttgggccgac
    22081 ctactgtgac cgagtgaccc ggctggtctg gaatgcgtcc tcggtggcga acagatagtt
    22141 gcggatgccc agcacgacct cgtcggcggc gacccaccga tcgtcctgcc agacgtacat
    22201 gccgtactcc ggccagcggg caagaatcga ctcggcgaac gagttgccgt ccttgccgta
    22261 gcgagccaga tggcggtcct cgatctcgag cagcaggctg ggatgatcgt tggtgatggt
    22321 ggcgacggcg ccctcgatca cctggggctc gaagccctcg acgtcgatct tgatgaaggt
    22381 gacccggtcg attccctggg ccttcaccca cgcatccacc gaggtgatca tggtgttcca
    22441 ctgccgcact cgcgcggtgg ggtgaaccgc cgccccctcg gtgatgtggg cgtggccgta
    22501 gatgggaaag ccgagcttga gcggcagccg catggtgcgc tcgccggtcg tcgcgcccac
    22561 cgccgcggca ttgacgctca cctgggacac cccgaccagc ccccggagga agcgcagggt
    22621 gcgctgctgc cgcgggtggg gttcgaagga ttccacccga ccggtcggtc ccacgaggtg
    22681 ggccagcggc agcgtgtaca tgccgtgcgc ggcgccgatg tcgatcaccg aatcaccggg
    22741 acgcacgatc gcccgcagac cacgcagctc ggcctcggcc cagttgatgc gagtcgcgaa
    22801 ctgcatgatc gagttcgcga gtctcggtct gagcgcggtt gcggtgggga ggacgggctc
    22861 ggggtaaacg tctgttgtca cgttctcttc ctcggtagtc cgaccagtac cctgccgccc
    22921 catcgtagtg ggtcaagacg gctagcctgt gggcatgcac ctgccgctca gccgcgcgat
    22981 cgccccgcac ctccacctgc tcgacgaggt ggggtccacc aacgccgagc tcgtcgcacg
    23041 ggcagcctca ggcgaagccc aggacttcac ggtcgtggtc accaccaacc agacggccgg
    23101 ccgcggccgt ctcgaccggg tgtgggtcgc gccgccgggg cgcacgatcg ccgtgtcggt
    23161 gctgctgtcg ctgccagacc tccccctcga gaaggtgggg tggatgccgc tgctcgcggg
    23221 cctcgccatg acccgcgccg tggcatccct cgtcgaccca ccccgggtct cactcaagtg
    23281 gcccaacgac gtgcaggtcg acgggctgaa ggtctcgggc ttgctcaccg agctgctcgt
    23341 ctccccgcga gccgtcgtga tcggcgccgg cctcaacctc acgatgaccg ccgaggagct
    23401 gcccaccccg gccgcgacct cgctgacgct cgtcggtgcg gacgcgaccg acatcgtgga
    23461 tcgcgcgctc gcggcctatc tcggcgagtt acgtcagctc agcgacgcct atcgcggctc
    23521 gggtttcgac cccgaggcgg gattgcgcga tcaggtcgtc agggtctgcg gcacgatcgg
    23581 acagcaggtg cgggtcgaac tgcccgacgg cagcatgctg cgcggggtgg ccgagggaat
    23641 cgactcggtg ggtcgccttc tggtgcgcga tgattcgggc gtgcagcccg tcgccgctgg
    23701 tgacgtgacg cacctgaggt atgaatgaga gcatgaccga ggcgccagtg cgggaggtgg
    23761 tcgtcgcgag gctccggcct cacgtgcggg tgctgttctg gccgaccgtg ttgctgctcg
    23821 ctgtctgcgc cgcgtacggc tacttcggcg gcaggtatgt cgacggctgg ccggccatcg
    23881 ccatcgcggc cgctgccggg gtgcttgtgc tcgtgggatg ggtgtacccg ctgctggcgt
    23941 ggctcgcgcg caactacacg atcacgaccc gtcgcaccgt cgtgcgcagc ggggtgttcg
    24001 tgcgcctgcg ccgcgaggtg ctgcactcgc gggtgcgctc cgtcaccatg cgttcgacct
    24061 ggctgcagag cctatttcgc agcggagatc tggaagcgga cctgggtggg gaccaggagg
    24121 tcgtattgag ggacgtgcca ggagcggtgc tcgtgcagga ggtacttcac gacctcattg
    24181 aggcgagccg accggactga cgctctccgc ctcaccggcc gccttcgcct cgagctccgg
    24241 acggaacacc cagatgcggt agaaggtgaa gcggaacgcg gttcccagcg cgaggcccac
    24301 gacattggtg gcgatgttgt cggcaagcgc cgaggtgaag ccgaggagat agtgcgacac
    24361 ccataggcac agcaacgcga tacccatgcc cccgacgctc accagggcga attccagacc
    24421 ctcgcgcagc acgtgcgatc ggcgcatcga gccgaacgtc cagtagcggt tgccgatcca
    24481 gttcgtgaca atggcgatcg aggtcgagat gaccttggcg atcaccggtc cctcgtggat
    24541 gagctctggt gcgagcacgg tgaggcgcaa cagattgaag atcccgacgt cgaccacgac
    24601 cccgacaagt cccacgacgc cgaagcgtac gaactggccg aacagggcgc gcatgcgcct
    24661 cctcaggtga gcgacaatgg gggagcgacg gcagtctatc tgtcgccatg ccaaggattt
    24721 ctgacaacga actggggagt accgtgagcc tgaccgtcgg tgtgatcggg ggcggccagc
    24781 tggctcggat gatgatcccg ccggcagtcg agctcggcat cggtctgcgg gtgctcgccg
    24841 agaccgaggc cagttcagcg ggcctcgccg catcagctat cggcgactac cgcgaccgcg
    24901 aggcggtgct cgcgttcgcc gacacagtgg atgtcgtcac cttcgatcac gagcacgtgc
    24961 cgcaggttct cctgcacgag ctagtcgacc gcggcgtgcg cgtgcacccc ggtccccagg
    25021 cgctgctcta cgcgcaggac aagttgctca tgcgggcgag gctcgccgag ctgggtctgc
    25081 cggtgccgga ttgggcggcc gtggcatccg ttcacgacct caccggattc atcgacgcgc
    25141 acggggggcg tgccatcgtc aagacgccgc ggggcggcta cgacggcaag ggtgtgcggg
    25201 tcgtcgccga tgcgcgcgac gtggccgact ggttcgacga cgatcaaccc ctgctggccg
    25261 aggaactggt cgagttccgg cgtgagctct cgcaggtgct cgctcgtcgc ccgtcaggtg
    25321 agatcgctct ctggccggtt gtggagtcgg tgcagcgcga gggggtctgc gcagaagtga
    25381 tcgcgccggc gccgcattcg gcgggccggg tcgctcaggt cgcggccgac atcgccacgt
    25441 cgatagccga gaatctggat gtcacgggag tgcttgccgt cgagctcttc gagacgacag
    25501 atgatcggct cctcgtcaac gagctggcca tgcgaccgca caacagcggg cactggtcga
    25561 tcgacggctc cacaacgagc cagttcgagc agcatctgcg cgccgtgctc gaccttcccc
    25621 tcggggcaac ggggagccgt gacccgtgga gtgtcatggt caacgtgctc ggcggggttg
    25681 cggacagcgc gcacgatgtg aggctcgccg cggcgatggc cgaccagccc gacgtcaagc
    25741 tccacttcta cggcaagcag gcccgacccg ggcgcaaggt cgggcatgtc acggccggcg
    25801 gtgccgacct cgacgacgtg gttttccggg cgcgtgccgc agcggcattc tttgaggatt
    25861 gacacctacg cttgacgggt gagtaccccc ctcgttgccg tcgtcatggg ctccgactcc
    25921 gactggagcg tgatgtccgc tgccgccgcc gtgctcaccg agttcgacat tgctcacgag
    25981 gtgcaggtgg tatcggcaca ccgcacgcca gccgcgatga tcgccttcgg ctcggcggct
    26041 gccgggcgcg gcatccgggt gatcatcgct ggtgccggcg gtgcggcgca cctgccgggc
    26101 atgctcgcct cggtgaccac ccttccggtc atcggcgttc ccgttcccct gaagacgctc
    26161 gatggtctcg attccctgtt gtccatcgtg cagatgcccg cgggcattcc ggttgccacc
    26221 gtgtcgattg gcggagcgag gaacgcgggc atcctggccg cgcgcatcct cgcctcgggc
    26281 gacaccgttc tgacggcgaa gctcgctgag ttcgccgctg cgctcgaggc ggaggtcgcg
    26341 cagaagaatg ccgccctcat cgcgtcgctg tgaggcgtcg gcatgactaa cgcttccagc
    26401 ccggttcgct atccgaatac gcgatctccg gaggtgatga cgcggcgcgc ctgggttctg
    26461 gtcgtgctca acatcctcat ccccgggtcg gcgcaactgc tcgccggcag tcgacgactg
    26521 gggcgattcg gcgtcgggtc gacgttcgtg ctctggggtc tcatcgcgat cggcgcgctc
    26581 gtgtacttcc tcgggcgcac cgctttcatc acgatcgcca ccaactcgtt cgccctctgg
    26641 gttctgcagg gcgtgatcat cttctacgtg gtgctctggg tggtgctcac cctcgacacg
    26701 ctgcgcctgg tgcggttcgt gaatgtcgag ggcaacgccc gcatcttcgt cgcggtgctg
    26761 agcgtggcgg cactcgtggt cgtcgcgggc acgggcgcct acgcggtcat gatcgccggg
    26821 accacccggt cgacggtggg cgaggtcttc gcaggaacaa ccatcgagcc ccccgtcgac
    26881 gggcggtaca acatcctgct tctgggtggc gacgccggac cggaccgcga cggcctgcgc
    26941 cctgacagca tctcggtggt gagcgtggag gcctcgacgg gcaaggcgac catcatcggc
    27001 gtgccccgca acatggagga cgtgcccttc gtcgaaggat cgccgctcgc ggccgagtac
    27061 cccgacggct acggcgtgaa cgactgccag gtcgacgtgt gcctcatcaa ctcgatctac
    27121 accgaggtcg agttgtactc acccgacctc taccccgatg cggtcgcgaa cggcagccag
    27181 cccggcatcg aggcgatgag ggatgccgtc gaaggcaccc tggggatcac catccagtac
    27241 tacgccctca tcgacatgca ggggttcgct gacctcatcg atgccctcgg gggcgtcacg
    27301 atcgatgtcg cacaccgact gccgatcacc gacgaggtcg acgagaacgg tcagccctac
    27361 accgatgtgc cgtggatcga gcccggcgtg cagctcatgg gtggggggac tgcgctggct
    27421 tacgcgcgca tccgtgccgg cacgaccgac tacgagcgga tgggtcggca gcgccaggtg
    27481 caggaggcga tcctccagca gttcgatccg gccaacgtgc tgaccaagtt ccaggcggtc
    27541 gcccaggcgg gtgcgcaggt cgtgagcacc gacatcccac agtcaatgct cggatatttc
    27601 gtcgaactgg caggcaagtc tcgcgagttg cccgtcgaca cgctcgacct cgtgcccgac
    27661 aacggcgtcg accccgagta tcccgacttc gactacattc gccagctcgt cgccgaagcg
    27721 ctggcgccga ccgtcgagga gaccccggcg ccttagcaac gctctacagg tcggcgtgca
    27781 gttgccagac cttgtcggct gccccgcgcc aggagaacac gccggcgcga tcgatgccct
    27841 cgacacgcag tcgatcggca agagtcgagt cggcgagaac ggaaccgatg gcctcggcga
    27901 gacgctcgga gtagccctca ccctcgcgct ccaccgtgag cccggcctcc gccccgagtt
    27961 cgacgagcgc gggggcatcc gaatggatga ccggagttcc cagcgcgaag gcgtcgagca
    28021 cgggcagtcc gaacccctcc tcgaggttgg ggtaggcgaa cacggcggcc cgggatagcg
    28081 cgacggacag tgccgggtcg gcgagtgctc ccaggccccg aacacggccg ggcgcgacgt
    28141 gcgcgccatc tgcaagggag cccagcgcag ggctggcggg ctcggtgccc gcgagcagga
    28201 gggcgacgtc atcgtcgaga gctgcgaggg ccgcgacgag ttggtcgagg ccgttctggg
    28261 cgccgatatc gccgatggcc agcacgtagc gatcggggag ctccagctcg gtggcgagcg
    28321 catccgcgtc ggccggaatg cgaatggccg agctcggggc accgccgatc actctgattc
    28381 gttcgccgac agccgcgatc gtcatgagct catcggcaac ggtgtggctg ggcacgacga
    28441 tcgcgtcggc gtacctctcg gcccgcttga gcatcgcctt gtgccacgac actcggcgcg
    28501 ggctgagcac ctgcggtgcg gtccaggcga gagtgtcgtg aaccgtcacg atgatctggt
    28561 cgccgtcatt gagacggtcg tgcttggcga ggggggcgaa gaggctgggg gcatgcacca
    28621 tgccggttcc cggcagccga gtgaacccgt gctgccaggc cgaactcagt tcgcgacgct
    28681 gaagcgcact cttgtacagg gtcgcaaggc cgggcagacg ctcggtgatg cgctcgtatt
    28741 ccggttcggt ggacgcggag accagacctt caatggcgac acccttcggt gccgtcgaga
    28801 tgagggctcg ggtcaactcc tcggcgtacc gggagactcc ccccggagcg cccgagagca
    28861 tctcgtcgac aatcacgcgc agtgtggtca tgcggacacc agagttccag gcaggcccac
    28921 catgcgcccc tccgatccgt gcggctccgt acaactgaag aatctcggat cgagacccgc
    28981 tagtgcaaca ttgtacgggc tggccgccaa tcgctaccgc tggctgggca cgcaggccgc
    29041 tcacatccgt cgcgatagta ttggctcagc agtgagtgcg atcaattcat cgcgactcga
    29101 tggggccgta cacggtgcac cttgccgagg tggtcgctgt gtgttccccg caggcgggag
    29161 gcagtcgatc gtggcgaagc atgtcctact catcgctgag gctcgcattg ccacggttca
    29221 gatcctcgag ctgattctgc accatctggg tcagaccaca acactgtccg tgagcagcaa
    29281 actcctcagt gaactcgagc cggaggactt caccgacgac acgtatccgc tgctcgtcag
    29341 gtcgtgcagt cccgaggcga gcagcctcgt ccgtgccttg tcgagcacgg gtgctccgtt
    29401 cgggttctac ctcgacgaca acttctggct cctcgacccc acgacccctc tcggcaggca
    29461 ctatgccgcc aaaccaaccc ggcgacgcct cgactcgatc gttcgcgaag ccaccccggt
    29521 catcgcatcg acgcctctgc tccgcgactt cctccgacca cgggcgcggg atgttcagca
    29581 gctcgattcc ttcttcgact tcgcacttgt gccccaggtg ctgcccccgc ggtcgtcgca
    29641 tgcgcggctg cgggtggggt tcgcggcgag cgcctcacgc ggtgacgacc tagcgacagt
    29701 cctcgccgac gtctgtgccg cgctcgagga gcatccgcag ctggagttcg aagtgatcgg
    29761 cgccgatccc gaagcgttgc ccgaccacga ccgcatcatt gcattcgact atctggcctc
    29821 gtacgccgag tatgtggcgt tccagcgcac gcgggaatgg gacatcggaa tcgcccctct
    29881 ggggggagcg acctccaacc gctacaagac cgacaacaag taccgcgaat acgcggctct
    29941 gggcatcgcg ggcatctatc aggagtcaga accctattcc gcggtacgtg acggggtgac
    30001 cggtctgatc gtcggtcgcg accgcggctg gcgcgaggca atcgatcgat actgcgccga
    30061 gccgggattg atagatgcgg tgcgtgcggc cgcacgacag gacgttgaag aaaggctctc
    30121 cctcgacgtg gtagcgccgc agtggggcaa gttcttctcg gccgcacccg ccatcggcga
    30181 tcgaccaggg cgggcagacg ccgttcggcg ggccctggca ccgccgtcga ccccccgtgc
    30241 cctccgtgct cagcgccgca gattgctctg ggcatacggt cgaactcatc tcgccgaacg
    30301 cggcttctgg ttcactctcg tacgcacagt ccgctacccc ttccgccgcc ggcgggagaa
    30361 gcctttctca cttttgcggt gatagtgtgc gttagtgacg accgcgaccc tcgaccggca
    30421 agacaccccg tctcgcatcg tcagactcag cttcttcctc ggctggcagg atgtgcggct
    30481 catgtatcgc cgcagcgtac tcggtcagtt ctggatcacc ctgtcgatgg cgatcacctt
    30541 cttggcgatc ggcagtgtct tcggcctgat cttccagtca cccgtcgtcg agtacttgcc
    30601 attcctcgga tgcgggctgg tgttctggaa cttcctgagc ctgatcgtca atgagggtgc
    30661 cgtctcattc atcgtcggtg atgccttcat ccgtcagttg ccactgccgc cgatcatctt
    30721 cttcctgcgc agcatctgga agacggtctt cgtgctgttg cacaacgcga tcgcgctgat
    30781 ccttctcctg ctcgtcttct cgccgccgcc cacccccgcc atcctgctcg tcttccctgc
    30841 agtcgtgctc gggggtgcag ggatggccgg actcgctctc gccctagcgg tacttgccac
    30901 acggttccgc gacgttccgc agattctctc tgccctcatc caggtgctgt tctacctgac
    30961 accgatcgtc tggctgccac agtcgattcc cgagcaggct cgaggcctga tcctcggaat
    31021 caacccgttc taccacatga ttcaaatcat gcgtcagccg ctattgggcc agctgcccac
    31081 cctgcaggag tggggaatcg cgggcgctct ggccgcagtc ttcatcgcac tcggcgcgct
    31141 ggtctatgcc cgcaactatc accgactggc gttctggctg tgatctcatg accgtgcata
    31201 ttcatcttga tgacgttgag ctcgactacc cgatcctcag tcagtcgtct cgcaggctcg
    31261 gccgccagct gatcaatgtg gcaacgggcg ggcagctcga ccgctccgaa cagggcagga
    31321 tcacggtccg cgccctgacg gacattaccc ttcggctgga ggtcggcgac cgcgtgggcc
    31381 tcatcggcca caacgggtct ggcaagtcga ccctgctcaa gcttctcggc gggatctacc
    31441 gtcccacctc cggaacggtc tcgcgtgcgg gtgtcgtcgg atccctgctc gacatcagtc
    31501 tgggcatgtc tcccgacgca accggccgcg agaacatctt tcttcgaggg gcactccagg
    31561 ggatgtcacg gtcggagatc gcgcgcctcg tccccggtat catcgagttc agcgagttgg
    31621 gcgacttcat cgaactgccc atgcgcacct acagctcggg catgcttctg cgcctcgcgt
    31681 tctcggtggc aacggtgctg tcgcccgaca ttctgctgat ggacgagtgg ctctccgtgg
    31741 gcgac
//
LOCUS       DAOSOU010000002        30248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_1536_length_30248_cov_7.120591, whole genome shotgun sequence.
ACCESSION   DAOSOU010000002 DAOSOU010000000
VERSION     DAOSOU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 30248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30248
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1536_length_30248_cov_7.120591"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLW15_00165"
     CDS             complement(<1..188)
                     /locus_tag="PLW15_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT32844.1"
                     /translation="MTNPVPEARTATATAPTPVVEVKGVVAGYLPGVNILNGCSLTAN
                     QGELIGIIGPNGAGKSTLL"
     gene            complement(169..1098)
                     /locus_tag="PLW15_00170"
     CDS             complement(169..1098)
                     /locus_tag="PLW15_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT32845.1"
                     /translation="MSSNVKPTRGAPVKSVNLHIGEIEPGVAKVDPIIVADHVTRSFG
                     GLTAVDVAHVEIPRNAITALIGPNGAGKTTFFNLLTGFDKPDTGRWTFLGKSLAGIPS
                     FRVARLGQVRTFQLTKALNLLSVLDNMKLGAKGQKGENMFVALLPWLWRKQEEANEAK
                     AMELLGRFKLDTKAEDYAASLSGGQRKLLEMARALMSDPTLVMLDEPMAGVNPALTQS
                     LLDHILDLKTQGMTVLFVEHDMQMVRHIADWVIVMAEGKIVAEGPPDAVMSNPAVIDA
                     YLGSHHDQDLGVDPHAEETMHALKELLDDEPSS"
     gene            complement(1088..2071)
                     /locus_tag="PLW15_00175"
     CDS             complement(1088..2071)
                     /locus_tag="PLW15_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT32846.1"
                     /translation="MVDFWQIIQNAAVELISPTTAAYALAALGLAIHFGYTGLLNFGQ
                     AGFMAIGAYGYAISTLTLGFPVWGAVLVGVGGSIIFALILGIPTLRLRADYLAIVTIA
                     ASEILRLIFTTNSLDAVTGSADGLSAYKGGFAALNPFPKGTYGFGPFTMNEYDLWVRV
                     VGWTLVILGGLLAWQLMRSPWGRVIKGIREDEDAVRALGKNVYFYKMQSLIIGGVFGT
                     LGGMIFIMPRAVVPANYATTLTFFIYTILLLGGAATILGPIVGSMIFWVLLSAVSGFV
                     ARAQAVGWFPGMSSVQAGQTRFIIVGVVLMLLVIFRPQGIFGNKKELAFVK"
     gene            complement(2081..3349)
                     /locus_tag="PLW15_00180"
     CDS             complement(2081..3349)
                     /locus_tag="PLW15_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT32847.1"
                     /translation="MRALMLLLVGMIAALTFVGGGAAHADEINTDKYDFSFSGFVRND
                     GDPLKGVLITVSGNGYSAQVKTDADGKWKIGVPEKGTYNITLDESTLPTGIIVAEGGN
                     SVDVEIDKVNNKVYNFFLGQGVRETTSFLDQFIQRAFTGLSFGLLLALAAIGLSLIFG
                     TTGLSNFAHAEMVTFGALATLTLAVDLAWPLWIALVGALLLSGMFGWGLDAAIWKPLR
                     RKGVGLIPMMIVSIGLSLALRYIYQYIYGGSTRQLPGADGVDLKFGAISLSPLDLLSM
                     AISIVVLIAVAFFLLRTRIGKATRAVSDNPSLAAASGIDVDRVIRIVWVIGGALAGLS
                     GILFAFYRPGVSWDMGFQILLLIFAAVTLGGLGTAFGALVGSLVVGFFIEISTLWIPS
                     DMKYVGALAILILVLLVRPQGILGRKERIG"
     assembly_gap    3582..3681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3682..>4841
                     /locus_tag="PLW15_00185"
     CDS             <3682..>4841
                     /locus_tag="PLW15_00185"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT32848.1"
                     /translation="YVDKVKRSESGMITNPVTTTPDATVAEVDALCGQFRVSGLPVVE
                     GDGRLVGIITNRDMRFVSSFEKSTTLVRDVMTKAPLITAPVGIDPDSAIAILAQHKIE
                     KLPLVDASGRLGGLITVKDFDKSEQYPNATKDDEGRLRVGAAIGFFGDAWDRAGALRD
                     AGVDVIVVDTANGDSAGVLDIIRRLKGDRAFDAIDVIGGNVATRSGAQALIDAGADAV
                     KVGVGPGSICTTRVVAGVGVPQVTAVYEASLAARDTGIPIIADGGLQYSGDIAKALVA
                     GAETVMLGSLLAGTDESPGDLIFVNGKQFKSYRGMGSLGALQTRGKKTSYSRDRYFQA
                     DVPSDEQLIAEGIEGQVPYRGPVSSVVYQLLGGLRQSMFYTGARTIDELRVKG"
     assembly_gap    4842..4941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4942..5931)
                     /locus_tag="PLW15_00190"
     CDS             complement(4942..5931)
                     /locus_tag="PLW15_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT32849.1"
                     /translation="MNAKLRVAVIGTGGWARQHARIFSRRPDTELVAIVGRDEARTAA
                     RASEYGTAAYTDIERMLAAEHPDLVTVCLPNEHHFGPTLQVIQAGVPLLAEKPLVFEL
                     DQANRLIREADDRNLFFGINFNHRYAEPVARAKAAVDAGELGELVFATWRFGGEANMA
                     DSPHANLIETQCHGFDMLEHLCGPIVSVAAQMTNKTYGAFSTMAIALEFADGAVGTML
                     GSYDSSYAYEDAQRVELNGTLGRAVIHDTVRSLTIEHAGDETAKVWRAGYFNDEARTF
                     EYTFDRHVDALLTALRAGHEPPVPAAAGRRALALALAVIESHETGRRVDTFGV"
     gene            6021..7709
                     /locus_tag="PLW15_00195"
     CDS             6021..7709
                     /locus_tag="PLW15_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT32850.1"
                     /translation="MGYIDVNGVTYTLADGRVLLDDVSFRVGEGTTSALIGANGAGKS
                     TLLRLLRGQIKADSGAVTLEGGLGVMDQFVGHVRDDSTVRDLLVDVSPTAVREAARAL
                     DASELALIETDSTANQMAYATALADYADVGGYDQETIWDTCTVAALGIPFERAKYREV
                     STLSGGEQKRLVLEALLRGPEQVLLLDEPDNYLDVPGKRWLEEALRSTPKTVLLVSHD
                     RELLARAADRIITLEVGGAGNTAWTHGGSFATYHEARGDRMARLDELRRRWDEQHTKL
                     KTLVYNLKVKATAMDSFASRYQAALTRLTKFEAIGPPQERPIEQDLQMRLRGARTGNR
                     AVVCEQLELVGLMKPFDLEVWFGERVAVLGSNGSGKSHFLRLLALGGSDPDPRAGHVT
                     SVGGTLAPVEHTGRAKLGARVVPGWFAQAHEHPTLLGRTLLDILHRGDDNRAGLGREA
                     ASRALDRYGLVEAAEQVFESLSGGQQARLQILLLELSGATLLLLDEPTDNLDLVSAEA
                     LEEALTRFEGTVLAVTHDRWFARSFDRYLVFGSDGRVYESDDPVWDESRVVRTR"
     gene            complement(7721..8521)
                     /locus_tag="PLW15_00200"
     CDS             complement(7721..8521)
                     /locus_tag="PLW15_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT32851.1"
                     /translation="MTRPLPLYRRSLGESWRSLIGWSLGFLAALLLYVPLFSSIGGNP
                     DMQALLDSLPPAMISALGYGAIATGAGYVSATFYGLMGFALSTIAAVAWSSAAIAGDE
                     ELGSLELTLAHGVTRTQVVLERSAAIITKLVWLNVLSVLVIFALNDSAGLGLEFGNLV
                     AVGAALLGISVLIAMVGIAAGALTGRRGWATGAAAGVAVASYALNAVANQSTDLDWLH
                     SWSPYAWAFHNAPLSDGADWGGLGLLYGVSAVLLAIAVVALNKRDVGI"
     gene            complement(8514..9431)
                     /locus_tag="PLW15_00205"
     CDS             complement(8514..9431)
                     /locus_tag="PLW15_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT32852.1"
                     /translation="MTLALETQALTKRYGRRLALDAVDLAVEPGTVFGVIGPNGAGKT
                     TTMRLLLDIIRPTSGSARVLGQDPRQGGVELRRRIGFLPGELRLEGRVTGRALLKHYA
                     EISGPVAPGVVDSLAQRLDLDLSRQVRKLSKGNKQKLGVVQAFMHQPDLLVLDEPTSG
                     LDPLVQQEFLTMVREAKNNGQTVFLSSHVLSEIQQAADSVAILRAGRIVTVSDVDTLR
                     ATALRRVNATIADATADAVRTAFARDQHLAELTITAGDSLTIAGTLEGDIDPFVKAIA
                     AFTVVDLRVEEPDLEESVLGLYAKESTDD"
     gene            complement(9428..10063)
                     /locus_tag="PLW15_00210"
     CDS             complement(9428..10063)
                     /locus_tag="PLW15_00210"
                     /inference="COORDINATES: protein motif:HMM:NF036526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT32853.1"
                     /translation="MRSTPVEDATTRSRIRDAAITLFARDGFVATSVRSIAEEAGVSA
                     GLVIHHFGSKEKLRAECDAWIVAELMKNKGGVDEGNLSASMQVMLSDLDQYRPTLDYL
                     GRMLLDGSEAGDRLLDDLVKLTETMLEEGVANGVMQSTSDPYMRAVLVTLHGVMPIVL
                     QRQFARMLGEPGLTATMIRRMTLPSLELYTHGLYRDATMLDAAREALKGNE"
     gene            10136..11257
                     /locus_tag="PLW15_00215"
     CDS             10136..11257
                     /locus_tag="PLW15_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOT32854.1"
                     /translation="MSDIEIGRAKRARRVYAFDDIAIVPSRRTRDPQDVSVSWTIDAY
                     TFDIPVLAAPMDSVVSPSTAIAIGKLGGLGVLDLEGVWTRYDDPEPVLAEIRSLPAAT
                     ATKRMQEIYSEPVKPELVTARLAEIRAAGVPVAGALSPQRTQELYKTVVDAGVDLFVI
                     RGTTVSAEHVSKTVEPLNLKKFIYELDVPVIVGGAATYTAALHLMRTGAAGVLVGFGG
                     GAASTTRASLGIHAPMATAVADVAGARRDYMDESGGRYVHVIADGGLGTSGDIVKAIS
                     VGADAVMLGSTLARATDAPGGGWHWGAEAHHPELPRGNRVEVGSLAPLAEILYGPSTA
                     ADGRVNLIGALRRSMATTGYSDLKEFQRVEVVVAPYHAG"
     gene            11261..12982
                     /locus_tag="PLW15_00220"
     CDS             11261..12982
                     /locus_tag="PLW15_00220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOT32855.1"
                     /translation="MPKSVTRSSKLGAAERAAAIEVLKAKELDILVIGGGIVGTGSAL
                     DAATRGLRVGIVEARDWASGTSSRSSKLVHGGIRYLEQLDFRLVREALIERGLLLQRI
                     APHLVKPVRFLYPLTKPVFERFYIGAGMLLYDLFSYTGGRPPGVPHHRHLTRRQVLRA
                     MPSLKANAFTGGLTYYDAQVDDARYVANLARTASFYGAHVASRVRVEGFIKVGERVVG
                     VQAHDLETGEKFEIRARQVVNATGVWTDDTQAMVGERGQFKVRASKGVHLVVPRDRFQ
                     SKLGLLLRTEKSVLFVIPWGRHWLIGTTDTDWDLDKAHPAATAADIDYLLEHVNKVLA
                     VPLTRDDVEGVYAGLRPLLAGESDQTSKLSREHIVAHSVPGLVVVAGGKWTTYRIMAK
                     DAIDEAVAALDGRISESITEDIALLGAEGYHAAWNKRGKLARAFGVHKVRIEHLLNRY
                     GVLTDELLDLIRERPELQEPLPGADDYIAAEVVYAATHEGALHLEDVLARRTRISIEA
                     WDRGVSAAPVVAELMAAELGWDAARTKREVNYYLKRVMAERESQLQPDDASADRIRLE
                     APDIVAS"
     gene            13043..13573
                     /locus_tag="PLW15_00225"
     CDS             13043..13573
                     /locus_tag="PLW15_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOT32856.1"
                     /translation="MVDVRRVKLPGVGVLHTFVTDDGGKVGVITHRSGHSDLITFSDA
                     EDGSDVSKVSLRLDEDEAHTLAELLGGTRITESLASLDAIPGLSIDWFTVDYEDHIAG
                     KKLGGLASRGVVGLTVVAVVRGDSANPAPADDFTVFPGDTLVVAGTPEKVAKAFAFYR
                     TGEMKSKVSADSPPGG"
     gene            13577..14977
                     /locus_tag="PLW15_00230"
     CDS             13577..14977
                     /locus_tag="PLW15_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT32857.1"
                     /translation="MQGHLGEELLVLGVLLVIAYVLGRLGKLIGLPAIPVYMLVGLMA
                     SPHTGWFPLNFESGNVELIAIFGLILLLFNLGLEFDQDEFFGNAGRLLVSGGTYILVN
                     MGVGFAFGFMVGWGTREALIIAGMTATSSSAIVTKLLIELNRLANKETPMILGVTVVE
                     DIFIAIYLAIVGVVLSGETEVWPVVLKLGIAFLFLVAMFTIARWGGRWVSKLFRTKDD
                     ELFTILFFGLAVAFGGIGELLGVTDAIGAFLIGLVLGATKYRARIEQFSLPLRDVFGA
                     FFFLNFGLVLDIGTFGEVLAPVAGAVVLTIVLNVVAGQFVAWLNKLGPQAGINATVIL
                     QNRGEFALILATLSLSAGLDERIVPFAGLYVLTMSIIGPILAVNSERIGAVILGTKRK
                     KAVVAPERDRIREEGIALVEAATAGQEASQPSDLDDAIALHVPEMAAAEPRDPVAEQA
                     AMQSDEVAERKKDPEY"
     gene            14974..15762
                     /locus_tag="PLW15_00235"
     CDS             14974..15762
                     /locus_tag="PLW15_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HOT32858.1"
                     /translation="MSTTVWQVARRPRWIAALVLALGVASAFAALGQWQLARSVEGGQ
                     VTEEQTESSVPLTSIAVPQKPMPSDTIGQLVTVGDVTWVAGDWIVLSDRSNHAEPGYW
                     VVAHAVTQTGASLAVALGWTASADAAASAITTLEATPPAASLSGRYLPTESPQQSDFE
                     AGKHSALAVSELVNLWADAPDGVYSGYVISDQAPAGLETIDAPPPNNEVSLNLLNIFY
                     AIEWVIFAGFAIFLWYRLVKDAWEEENLPQESPAEPSPQEPTLN"
     gene            15767..16186
                     /locus_tag="PLW15_00240"
     CDS             15767..16186
                     /locus_tag="PLW15_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOT32859.1"
                     /translation="MPLGPRPDDIPRIRRTVSVYKVSSIITGSFLLLLVLMMVLRYGF
                     GVDIELLGPYGFLALTPKELIAGINLSTVILIVHGWLYVLYLACDFLLWRYIRWSFAR
                     FLFIALGGIIPLLSFFLERQVPRQAEAAIASIEKATA"
     gene            16183..>16535
                     /locus_tag="PLW15_00245"
     CDS             16183..>16535
                     /locus_tag="PLW15_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203631.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT32860.1"
                     /translation="MTTVPASSTEARPVLVVDFGAQYAQLIARRVREANVYSEIVPHT
                     ITAAEVAAKHPVGIVLSGGPSSVYEPGSPDLDPGILELGIPVLGICYGFQVMARQLGG
                     EVANTGLREYGATDMT"
     assembly_gap    16536..16635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16636..17927
                     /gene="guaA"
                     /locus_tag="PLW15_00250"
     CDS             <16636..17927
                     /gene="guaA"
                     /locus_tag="PLW15_00250"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT32861.1"
                     /translation="GGEVANTGLREYGATDMTVTGGGGALLSGQPEAQTVWMSHGDSV
                     SRAPEGFEVLASSESTPVAAFASDERRLYGVQWHPEVKHSQFGQRVLENFLHTAAGIP
                     ADWNSGNVIAEQVARIREQVGSAKVICGLSGGVDSAVAAAIVHKAVGDQLTCIFVDHG
                     LLRQDERRQVEEDYVASTGVRLVTVDAVDQFLNELAGVTDPETKRKIIGREFIRSFEA
                     AAADLVAEAASDGEPIKFLVQGTLYPDVVESGGGTGTANIKSHHNVGGLPEDLQFELV
                     EPLRTLFKDEVRAIGRELGLPEVIVSRQPFPGPGLGIRIVGEVTRDRLELLRAADAIV
                     RAELTAAGQDAAIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDAMTADWTRV
                     PYEVLAKISNRITNEVPGVNRVVLDVTSKPPGTIEWE"
     gene            18046..19809
                     /locus_tag="PLW15_00255"
     CDS             18046..19809
                     /locus_tag="PLW15_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOT32862.1"
                     /translation="MNDARPPAIVSLERLRSSLADPGLSLPLEGAATQRDAAASAVRQ
                     IDDYIRPRLADLDAPLLAVVGGSTGSGKSTIVNAIIGTPTTRAGVIRPTTRQPILVHA
                     PVDAGWFASDRILPGLARVRGRITEPGTPASSAGDVPTASRMNELMLLASDQVPASLA
                     LVDAPDIDSVADENRALANQLLAAADLWLFVTTANRYADAVPWALLDGAASRAITVAV
                     VLNRVPPGAADEVLPDLKQMLSSRGLPDAPVFVIEEQPLDELGMLPAESVAGPRTWLT
                     GIAADRVERARIAARTLRGAIVDLDIRVASIATARQQQLDWLDGIAKSVALDYDNAAD
                     AVDDATRDGALLRGEVLARWQDFVGTSDLFRSVERWFSRARDAATAFVMGKPQPVIDV
                     EVRIEHGLHAVIVDQAGRAAAASWAGLQRTEAGRAIAQAHPGLGTESPEFSEKAAAMI
                     REWQGALLRLIQDNAGSKRIRARVLSAGLNVVTVALMIVVFASTGGLTGGEIVIAGGS
                     AVLGQKLLETIFGEDAVRRLAGEARKDLTKRVGALFEREAARVAAALEAARFGSSPDS
                     LRRESAALLDDVRRAADGGTA"
     gene            19806..21383
                     /locus_tag="PLW15_00260"
     CDS             19806..21383
                     /locus_tag="PLW15_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOT32863.1"
                     /translation="MKVTLDQRLAALRTLRETGAGRIPDALARDLDAVLTHSGERRQR
                     SPEHTVVGIFGATGSGKSSLVNALVGADVARTHVRRPTTSEAHAVVWDPDGAVDLLDW
                     LQVRERTVRDGPIDARAGKLVLLDLPDFDSVERANREIAERLAAQVDALVWVVDPQKY
                     ADEVLHAQFIAPHAKHSAVTLIVLNQIDLLPAADVPHVVDSLRGIVERDGIPKARVLS
                     VSARTGEGIDALRKALGDLAAATEVREARLAADVSTVVARVPAAGTVTRPGKGAIDTL
                     VTDLGTAAGADVVAQAVARSYRKRAGQATGWPIVSWLLRLRPDPLARLGLGPKRRGEN
                     PDLHRTSMPALDAGARARVSLSVRGFADAAAAGLTDAWRTGIRTTADRAIADLPDQLD
                     LAIARTNIPAKGSWWWVIFSVLQWVSIVAALSGIVWLLGAAFLPTFGLPAFQVPKVEG
                     WSVPTLLIAGGVALGILLGMLGALLAAIAASSRRRRTRKLLTASVRTVVQDTAVAPLV
                     ADLDRASAYTAALAQAG"
     gene            complement(21606..21848)
                     /locus_tag="PLW15_00265"
     CDS             complement(21606..21848)
                     /locus_tag="PLW15_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32864.1"
                     /translation="MILEQEPWVGLVIGGCFALWGLAFALFRRRISRAAIAQRMEWDK
                     GRGVKFQHPVVIGIGGSVFCLCGVALAVVSLVRLLT"
     gene            complement(22115..22927)
                     /locus_tag="PLW15_00270"
     CDS             complement(22115..22927)
                     /locus_tag="PLW15_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOT32865.1"
                     /translation="MAGSNPAFTRSPAFSAQAGGVAVAQDISAQQLDEIYNRSSAPAE
                     GETMTVENTIVKTVISFILLLAGAAIGWITAPAVPLLWIGAAIVAFVLALVNIFKKEP
                     SAPLILAYAAAEGVFIGGISAFYEAMWPGVVVQAVIATLVVVGVTLALFASGKIRASA
                     KATKVFLIAMVGYLVFSLVNVGIMIFGGAGGNPWGLRGSVEIFGIPLGVILGVFVVIL
                     AAYSLVLDFDFIQRGVNNKAPAKYGWTGAFGIMVTVVWLYLELLRMFAIARN"
     gene            complement(22955..23527)
                     /locus_tag="PLW15_00275"
     CDS             complement(22955..23527)
                     /locus_tag="PLW15_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32866.1"
                     /translation="MSGSEPPAAPWGPAVWREPTSLIPLKWGRYTDRYLWGLILIISG
                     TTAMQAGNIYAVSFLGQGIVALVIGWSIMPARGWRRTLAAFLAPLFAVALLTGPQSVW
                     TLAAPYLLWLCVRHRPLRSYITVLFPIANGFIVPQFFEEYDGMPLALAISMVVFVASA
                     WLARLIARQGAAAAETPNDPPFPSKTPVDL"
     gene            complement(23514..23732)
                     /locus_tag="PLW15_00280"
     CDS             complement(23514..23732)
                     /locus_tag="PLW15_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOT32867.1"
                     /translation="MSTCTSCEAELRPEWKFCIMCGTPVIPAAVRPERAETAVGEKIA
                     TYAPLVFAIACFSLAAIVIAWFAINVWF"
     gene            23750..24751
                     /locus_tag="PLW15_00285"
     CDS             23750..24751
                     /locus_tag="PLW15_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT32868.1"
                     /translation="MTVARVPLIIGHRGASGYRPEHTRAAYELAFRLGADAVEPDIVA
                     TRDGVLVLRHENEISGTTDVASRPEFADRRTTKLIDGARLTGWFTEDFTWAELSTLRA
                     VERLPQVRQASATFDGLEPILRLRDLLGIIDDASGDRQRIMVAELKHATYFESIGLPL
                     DELFASEVSGWASDENLIVECFEQTVLGKVRARGVPGRVVFLAEASGSPADLVAQFGS
                     KALPYSAHLTNAGLARLATAVDGVSVDKRLILSTDAAGTTTTTDFVERAHAAGLEVYT
                     WTLRAENKFLAKGMRRGSSARDYGDWLDEFRLILGTGLDGVFADQPDLVREALVSGS"
     gene            24821..27199
                     /locus_tag="PLW15_00290"
     CDS             24821..27199
                     /locus_tag="PLW15_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT32869.1"
                     /translation="MTYVLEPFDQADPPGDGRPGRLDSAILDGLNPQQREAVEYRGQS
                     LLIVAGAGSGKTSVLTRRIAGLLQTREAWPSQILAITFTNKAAAEMRERVKSLVGDAA
                     EGMWISTFHSACVRILRRQAEAFGMKQSFTIYDTQDSRALMKRIIKELDADTLGFTAG
                     GALNRISKLKNELTDVEAFGRTANLSDPKEAVLLEIFRQYSASLKRANALDFDDIIGE
                     TVYLFRAFPDVAALYQRRFRHVLVDEYQDTNHAQYALIKELVKAVRPQHVPPDTRMPL
                     APDGSIPPASLTVVGDSDQSIYAFRGADIRNIVEFERDYPNSKVILLEQNYRSTQNIL
                     DAANAVISNNFDRKAKNLFTVVGEGEKIVGYTGYSGHDEAQFVADEVEVLRSQGLNLN
                     DIAVFVRTNSQTRPLEEIFIRSALPYRIPGGTKFYERAEIKDAMAYLIQVANPDDDLA
                     LRRIMNVPKRGIGPATEAALQAYADRVGVPLREALVDAPQLGLGPKVTAAIVDLGTIL
                     DEVAASAATAAPADILTELLNRTGLHRALKASGDPQDEARAENLEELVSQTKEFRTAN
                     PEGTLVDFLTGIALFAAADEIDDSGGSISIMTLHTAKGLEYDAVFLTGLEEDLLPHRM
                     SSNEPGGLAEERRLFYVGITRARKRLYLSLAASRASFGDVNIAMPSRYLQEIPTELIE
                     WRQSPGMANSRGGTRPRALNARSGTGFSYGQSLPPAPTRVKTEWTSAVTGAVRDNGDL
                     TLAVGDRIRHTDFGDGSVTAVTGEGTKKVAEVQFDVAGRKRLLIKIAPIEKL"
     gene            27250..28662
                     /locus_tag="PLW15_00295"
     CDS             27250..28662
                     /locus_tag="PLW15_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOT32870.1"
                     /translation="MPDAQDELVAALRVIREELELPESFPAEVEAEATRAAARASRPE
                     NDLTGIPFITIDPASSTDLDQALHLERDGDGYRVRYAIADVPAFVEPGGAIDTEARLR
                     GQTLYAPDGRIPLHPTVLSEGAASLLEGVDRPAFVWTFALDATGTVTATTLERALVRS
                     RRKCSYIDAQADIDAGTADASLALLPVIGELRLALERERGGASLDRPEDEVAVTDGRY
                     ELVRRAPLPIESYNAQLSLMTGMAAAQFMLDGRVGILRTMPAPDPETIARFRAQVQAI
                     GCPWPEQQPYGEYLRALDRDDPRGLAVLHAAGALFRGAGYRAFDGEVPEDTIQAAIAA
                     PYAHATAPLRRLVDRFVLVTCEALANGRPVPGWVREALPTLPAIMSRSDSLASRLDRA
                     SIDAIEAALLRDRVGETFEAIVIATTAAGGTVQLSDPFITAAVEGDVRPGQRVKATLV
                     TADIATGATRFRVAGLGVVD"
     gene            complement(28669..29907)
                     /locus_tag="PLW15_00300"
     CDS             complement(28669..29907)
                     /locus_tag="PLW15_00300"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT32871.1"
                     /translation="MRFSALRAPWYKGYLAGGSLGMGADHVEHAITYWVIWQAFHSPL
                     LAGFAVISHWLPHLLFGVIFGGLADRYDCRRIVQIAQGLFMLASLGWAIVIITGTLQP
                     WNAVLLLSLHGLASALWGPAEQMMLYDIVGPEDLPSAVRLMATGLNLGMLVGPLIGAA
                     LLFTVGPAWGLLVNIALYLPFIIYLGIVPYTGHTRAGQTPRPRLTLRQTFGVLGEIPR
                     YPSVLTVFILQGAVGLFIGTALLPLLPDFGELLGATGSGVGYGALIAAMSFGAVTAGV
                     SLEAIGRIRATAGLAIVSTIVFAASLIVFALSHNLVLSVAMLVIAGMGNLTSASTSQA
                     VVQLGAPADRRGRFLGAYSMTSMGLRLGSGVLIGVLASAFGPSVAIGVDAAALLILAT
                     SLLAVVLVRRASARPAATQP"
     gene            complement(29904..>30248)
                     /locus_tag="PLW15_00305"
     CDS             complement(29904..>30248)
                     /locus_tag="PLW15_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HOT32872.1"
                     /translation="GVPRALWPADRAAFDAYWSRCVDELAVDAAAAHVARELLHPRRG
                     PLWLRLAMPTVRTVTAGLLDQPLRSAYGLPFVERRYQRLVRIVRFVYPRLPRWVRHAP
                     QRHYLKAFRLGR"
BASE COUNT         4908 a        10206 c        10236 g         4598 t
ORIGIN      
        1 agcagcgtcg acttgccggc gccgttcggg ccgatgatgc cgatcagctc gccctggttg
       61 gcggtcaggg agcagccgtt gaggatgttg acgccgggca ggtagcccgc cacgacaccc
      121 ttgacctcga ccaccggcgt gggagccgtc gcggtcgcgg tgcgagcctc aggaactggg
      181 ttcgtcatcg aggagctcct tcagtgcgtg catggtctct tcggcgtggg gatcgacgcc
      241 gaggtcctgg tcgtggtggc tgccgaggta ggcgtcaatc actgcagggt tggacatcac
      301 ggcgtcgggc gggccctcgg cgacgatctt gccctccgcc atcacgatga cccagtcggc
      361 gatgtgacgc accatctgca tgtcgtgctc gacgaacaac accgtcatgc cctgcgtctt
      421 caggtcgagg atgtgatcga gcagagactg cgtgagcgcg gggttgacgc ctgccatcgg
      481 ctcgtcgagc attaccagcg tcgggtcgct catgagcgcc cgagccatct cgagcagctt
      541 gcgctggccg ccggagaggc tggccgcgta gtcctcggcc ttggtgtcca gcttgaagcg
      601 ccccagcagc tccatggcct tcgcctcgtt ggcctcctcc tgcttgcgcc acagccaggg
      661 caggagcgcg acgaacatgt tctcgccctt ctgccccttg gcgccgagct tcatgttgtc
      721 gagcacactc agcaggttca gtgccttggt gagctggaag gtgcgcactt gacccagtcg
      781 ggccaccctg aatgacggga tgccggcgag cgacttgccc aggaacgtcc atcgacccgt
      841 gtcgggtttg tcgaatccgg tgaggaggtt gaagaacgtg gtcttgcccg caccgttggg
      901 cccgatcagc gcggtgatcg cgttgcgcgg gatctccacg tgcgccacgt cgacggccgt
      961 gagaccgccg aaactgcggg tcacgtgatc ggcgacgatg atggggtcga ctttggccac
     1021 gcccggctcg atctcgccga tgtgaaggtt gaccgacttg accggggcgc cccgggtcgg
     1081 cttgacgtta cttgacaaaa gccagctcct tcttgttgcc gaagatgccc tgaggtctga
     1141 atatcaccag gagcatgagc accacaccca cgatgatgaa tcgcgtctga ccggcctgca
     1201 cgctcgacat cccggggaac cagccgacgg cctgagcgcg ggcaacaaag cccgacacgg
     1261 ccgacagcag cacccagaag atcatcgatc cgacgatcgg tccgagaatg gtcgccgccc
     1321 caccgagcag caggatcgtg tagatgaaga aggtcagcgt cgtcgcgtag ttcgcgggca
     1381 cgaccgcccg cggcatgatg aagatcatgc cgccgagggt tccgaacacg ccgccgatga
     1441 tgagcgactg catcttgtag aagtagacgt tcttgccgag ggcgcgcacg gcatcctcgt
     1501 cctcacggat gcccttgatg acccggcccc acgggctgcg catgagctgc catgcgagca
     1561 ggccgccgag gatgaccaga gtccagccca cgacgcgcac ccacaggtcg tactcgttca
     1621 tcgtgaacgg gccgaagccg tacgttccct tcgggaacgg gttcagggcc gcgaagccgc
     1681 ccttgtaggc gctgaggccg tctgcggagc ctgtgacggc atccagagag ttcgtggtga
     1741 agatgagcct gagaatctcg gatgccgcga tcgtcacgat cgccagatag tccgctctca
     1801 gccgcagggt ggggataccg aggatgaggg cgaagatgat cgacccgccc acgccgacga
     1861 gcaccgcgcc ccacaccggg aacccgaggg tcagcgtgga gatcgcgtag ccgtaggcgc
     1921 cgatggccat gaagcccgcc tgaccgaagt tgagcaggcc cgtgtagccg aagtggatcg
     1981 cgaggccgag ggctgcgagg gcgtaggcgg ccgtcgtcgg gctgatgagc tcgacggcgg
     2041 cgttctgaat gatctgccag aaatcaacca tgtctgtctc ctagccgatt ctctctttgc
     2101 ggcccaggat tccctgtggc ctgaccaaca gcacgaggat gaggatcgcg agcgctccga
     2161 cgtacttcat gtcggagggg atccacagtg tcgagatctc gatgaagaac ccgacaacga
     2221 gcgagccgac gagtgcgccg aacgcggtgc cgaggcctcc gagggttacc gccgcgaaga
     2281 tcagcagcag gatctggaag cccatgtccc acgagacgcc cggacggtag aacgcgaaca
     2341 ggattccgct gaggcctgcg agcgcgccgc cgatgaccca gacgatccgg atgacgcggt
     2401 ccacgtcaat gccgctggcc gcggcgagac tcgggttgtc gctgaccgct ctggtcgcct
     2461 tgccaattcg ggtgcgcagc aggaagaacg ccacggcgat gagtacgacg atggagatcg
     2521 ccatgctcag caggtcgagg ggcgagagcg agatggcgcc gaacttgagg tcgaccccgt
     2581 cggcgccggg aagctggcgg gtcgatccgc cgtagatgta ctggtagatg tagcgcagcg
     2641 ccaaggagag accgatgctg acgatcatca tcgggatgag gcccacaccc ttgcggcgca
     2701 gcggcttcca gatcgcggca tccagacccc agccgaacat tccgctgagc agcagggccc
     2761 cgacgagggc gatccacagc ggccaagcga ggtcgacggc gagggtcagg gtggccagag
     2821 caccgaaggt gaccatctcc gcgtgggcga agttgctgag gccggtcgtg ccgaagatca
     2881 gcgagagtcc gattgccgcg agggcgagca gtagaccgaa gctcagtccg gtgaaggccc
     2941 gctggatgaa ctggtcgagg aagctcgtcg tctcacgaac cccctgcccg aggaagaagt
     3001 tgtagacctt gttgttgacc ttgtcgatct cgacatcgac cgagttgccg ccctccgcca
     3061 cgatgatgcc ggtgggcagg gtcgactcgt cgagagtgat gttgtaggtg cccttctcgg
     3121 gcaccccgat cttccacttg ccgtcggcgt cggtcttcac ctgggccgag tagccgttgc
     3181 cggacaccgt gatcagtacg cccttgagcg gatcaccgtc attgcgcacg aagccgctga
     3241 agctgaagtc gtacttgtcg gtgttgatct catccgcatg ggccgcgcca cctcccacga
     3301 aggtcagggc ggcgatcatg ccgacgagca acagcatcag ggcgcgcatc cagcgcctgt
     3361 cagtcaaacc agtgacttcc acggaacctc caagacggac cagtcggaat atgggtatcc
     3421 cgcgggttct ggcgcaccgt cgacgttgac gatacttcct gattgtgtct ccagtgtttc
     3481 gtcggcgaca caaaccggtc aggccattat gcgcgcggaa tagcctcggg tcgaacccgg
     3541 ttaagattgg gtatggacca gcccgacccc tttggcttca tnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn ntacgtcgac aaggtcaagc gcagcgagtc gggcatgatc
     3721 accaacccgg tgaccacgac gcccgacgcg accgtcgccg aggtcgacgc gttgtgcggt
     3781 cagttccgcg tctcagggct gccggttgtg gagggcgacg gtcgcctggt cggcatcatc
     3841 accaaccgcg acatgcgctt tgtctcctcc tttgagaaga gcacgaccct cgtcagggac
     3901 gtcatgacga aggcccccct gatcactgct cccgtgggca tcgaccccga cagcgcgatt
     3961 gccatcctcg cccagcacaa gatcgagaag ctccctctgg tggatgcgag cggtcgtctc
     4021 ggcgggctca tcacggtcaa ggacttcgac aagagcgagc agtacccgaa cgccaccaag
     4081 gatgacgagg gacgtctgcg ggtcggcgcg gcgatcgggt tcttcggtga tgcctgggac
     4141 cgcgcgggcg cgctccgcga cgcgggcgtc gacgtgatcg tcgtcgacac cgccaacggt
     4201 gactcggcag gagttctcga catcatccgt cggctcaagg gcgaccgcgc cttcgacgcg
     4261 atcgatgtca tcggtggaaa cgtggcaacc cggtcgggag cccaggctct catcgatgcc
     4321 ggtgccgacg ccgtgaaggt gggcgtgggt cccggctcga tctgcaccac ccgcgtcgtg
     4381 gccggagtcg gcgtgcccca ggtcaccgcg gtgtacgagg catcgctcgc ggcccgcgac
     4441 acgggcatcc cgatcatcgc cgacggcggc ctgcagtact ccggcgacat cgccaaggcg
     4501 ctcgtcgcgg gtgcggagac cgtgatgctc gggtcgttgc tggcgggcac cgacgagagc
     4561 ccgggcgatc tgatcttcgt caacggcaag cagttcaaga gctaccgcgg catgggctcg
     4621 ctgggtgcgc ttcagacccg cggcaagaag acgagctact cgcgtgaccg ctacttccag
     4681 gcggatgtgc ccagcgacga gcagctcatc gccgagggca tcgagggcca ggtgccctac
     4741 cgcggaccgg tctcgtcggt ggtctaccag ctgctcggcg gcctgcggca gtcgatgttc
     4801 tacaccggtg cgcgcaccat cgacgagctg agggtgaagg gnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn ntcacacgcc gaaggtgtcg acccggcgtc cggtttcgtg
     4981 ggactcgatc acggcgaggg ccagagccaa tgcccgacgc ccggcggcgg ccggcacggg
     5041 tggctcgtgg ccggcgcgca gtgcggtgag cagggcatcc acgtgccggt cgaaggtgta
     5101 ctcgaacgtg cgcgcttcgt cgttgaagta accggcccgc cacaccttcg ccgtctcgtc
     5161 gccggcgtgc tcgatcgtca gtgagcgcac cgtgtcgtgg atgacggccc gcccgagcgt
     5221 gccgttgagc tcgacccgct gcgcgtcctc gtacgcgtag ctcgagtcgt agctgccgag
     5281 catcgtgccg accgcgccgt ctgcgaactc cagggcgatg gccatcgtgc tgaacgcgcc
     5341 gtaggtcttg ttggtcatct gggcggcgac cgagacgatc ggtccgcaca ggtgctcgag
     5401 catgtcgaag ccgtggcact gggtctcgat gaggttggcg tgcggggagt cggccatgtt
     5461 cgcctcgccg ccgaaccgcc aggtcgcgaa caccagctcg ccgagctcac ccgcgtcgac
     5521 ggcggccttc gcgcgcgcca ccggctccgc gtagcggtgg ttgaagttga tgccgaagaa
     5581 gaggttgcgg tcgtcggcct cgcggatgag ccggttggcc tggtcgagtt cgaacacgag
     5641 cggcttctcg gcgagcaacg gaactccagc ctgaatcacc tgcagggtcg gcccgaagtg
     5701 gtgctcgttg ggtaggcaga cggtgacgag gtcgggatgc tcggccgcaa gcatccgctc
     5761 aatgtcggtg taggccgccg tgccgtactc cgaggctcgc gcagcggtgc gcgcctcgtc
     5821 ccggccgaca atggcgacga gctcggtgtc gggcctccgg gagaagatcc gcgcgtgctg
     5881 gcgggcccac ccgccggttc cgatgacggc aacacgcagc ttcgcgttca tgcggccagc
     5941 gtagtgcgca ggggtgccca gcgtcgggga agttgtattc tggtcggctg cccagatggg
     6001 gcggctgaga gattcgggcc atgggctaca tcgacgtcaa cggcgtcacc tacacactcg
     6061 ccgacggacg cgtgctgctc gacgacgtct cgttccgcgt gggcgagggc acgaccagcg
     6121 cactgatcgg cgccaatggc gcgggcaagt cgacgctgtt gcggttgctg cgtggccaga
     6181 tcaaggccga ttcgggtgcc gtcactctcg agggcggctt gggagtgatg gaccagttcg
     6241 tcggccacgt gcgtgacgac tcgacggtgc gtgacctgct cgtcgacgtg tcaccgacgg
     6301 cggtgcgcga ggccgcccgg gcgctcgacg cgagcgagct cgcgctcatc gagaccgatt
     6361 ccacggcgaa ccagatggcg tatgcgacgg cgctcgccga ctacgcagac gtgggcggct
     6421 acgaccagga gacgatctgg gacacctgca ccgtggcggc tctgggtatc cccttcgagc
     6481 gcgcgaagta ccgggaggtc agcacgctct cgggtggcga gcagaagcgg ctcgtgctcg
     6541 aggctcttct tcgcgggccc gaacaggtgc tcctgctcga tgagcccgac aactacctgg
     6601 atgtcccggg caagaggtgg ctcgaggagg cgttgcgctc gacacccaag actgtgctgc
     6661 tggtgagcca cgaccgcgag ctgctggccc gggcggccga ccgcatcatc acgttggagg
     6721 tgggaggcgc aggcaacacc gcgtggacgc acggtggcag cttcgcgacc taccacgagg
     6781 ccaggggcga ccggatggct cggctcgacg aactgcgtcg gcgctgggac gagcagcaca
     6841 cgaaactgaa gacgctcgtg tacaacctga aggtgaaggc gacggcgatg gacagcttcg
     6901 cctcgcggta tcaggcggca ctgacccgcc tcacgaagtt cgaggccatc ggcccacccc
     6961 aggagcgacc catcgagcag gaccttcaga tgcgcctcag gggagcgcgc acgggcaacc
     7021 gggctgtggt gtgcgagcag ctggagcttg tcggactcat gaagcccttc gacctggagg
     7081 tctggttcgg cgagcgcgtt gccgtgctgg ggtcgaatgg gtccggcaag tcgcacttcc
     7141 tgcggcttct cgcgctcggc ggtagcgatc cggacccgcg cgccggtcac gtcacgagcg
     7201 tgggtggcac gctcgcgccc gtcgagcaca ccggccgcgc gaagctcggg gcacgagtgg
     7261 tgcccggctg gttcgctcag gcccacgagc atccgaccct cctggggcgc accctgctcg
     7321 acatccttca ccggggtgac gacaaccgcg cgggactcgg acgcgaggcc gcgagtcgcg
     7381 cgctcgaccg gtacgggctg gtcgaggcag cagagcaggt cttcgagtcg ctgagtggcg
     7441 gccagcaagc gcggcttcag atcctgctgc tcgaactcag cggggccacg ctgctgcttc
     7501 tggatgaacc gaccgacaac ctcgacctcg tctcggccga ggcgctcgaa gaggccctca
     7561 cccggttcga gggaacggtt ctggcggtca cacacgatcg ctggtttgct cgctcgttcg
     7621 atcgctacct cgtgttcggc agcgacggcc gcgtctacga gtctgacgac ccggtctggg
     7681 atgagagccg ggtcgtcaga actcggtgac ggatgccgcg tcagatcccc acgtcgcgct
     7741 tgttgagcgc cacgacggcg atcgcgagca gcacggcaga aaccccgtag agcagaccga
     7801 gtccgcccca gtccgcgccg tcgctcagcg gcgcgttgtg gaacgcccac gcgtagggag
     7861 accaggagtg caaccagtcg aggtcggttg actggttggc gaccgcgttg agcgcgtagc
     7921 tggctaccgc gactccggcc gcagcaccgg tggcccatcc gcgtcgacct gtcagtgctc
     7981 ccgcggcgat gcccaccatg gcgatgagca cgctgattcc gaggagggcg gcaccgacgg
     8041 cgacgaggtt accgaactcg aggccgagac ccgcgctgtc gttgagagcg aagatcacca
     8101 gaacgctcag cacgttgagc cagaccagct tggtgatgat ggcggccgaa cgttcgagca
     8161 cgacctgggt gcgggtcacc ccgtgcgcga gcgtgagttc gagcgagccg agctcctcat
     8221 cgccagcaat ggcggctgaa ctccaggcga cggccgcgat cgtgctgagc gcgaagccca
     8281 tgaggccgta gaaggtggca gagacgtagc cggcaccggt ggcgatcgca ccgtagccga
     8341 gggcgctgat catcgcgggt ggcagcgagt cgagcagcgc ctgcatgtcg gggttgccgc
     8401 cgatcgacga gaagagcggt acgtacagca gcagcgcggc gaggaacccg aggctccacc
     8461 cgatgagcga gcgccaggac tcgccgagtg atcggcggta gagcggcagc ggcctagtca
     8521 tcggtcgact ccttcgcgta cagtccgagc actgactcct cgaggtcggg ctcctcgacc
     8581 cgcaggtcga ccacggtgaa ggcggcgatc gccttcacga acgggtcgat atctccctcc
     8641 agggttccgg cgatggtcag ggagtcgccg gcggtgatcg tgagctcggc gaggtgctgg
     8701 tcgcgagcga aggcggtgcg cacggcatcg gccgtggcat ccgcgatcgt ggcgttgaca
     8761 cgcctcaggg ccgtggcccg tagcgtgtcg acgtcactga cggtcacgat gcgtccggcg
     8821 cgcaggatcg cgacggagtc ggccgcctgc tggatctcgc tgagcacgtg cgaggagagg
     8881 aatacggtct ggccgttgtt cttggcttcg cgcaccatcg tgaggaactc ctgctgcacg
     8941 agcgggtcga gcccactcgt cggctcgtcg agcacgagca ggtcgggctg atgcatgaac
     9001 gcctgcacga cgccgagctt ctgcttgttg cccttggaga gcttgcgcac ctgacggctg
     9061 aggtcgaggt cgagtcgctg ggcgagactg tcgacgacgc ccggcgcgac cggcccactg
     9121 atctccgcgt agtgcttgag cagcgcccgc ccggtgacgc ggccctcgag tcgcagttcg
     9181 ccgggcagga acccgatgcg gcggcgcagc tcgactccgc cctggcgggg gtcctggccg
     9241 agaactcggg ccgaacccga cgtggggcgg atgatgtcga gcagcaagcg catcgtcgtg
     9301 gtcttgccgg cgccgttcgg cccgatgacg ccgaagacgg tgccgggttc gaccgcgagg
     9361 tcgacggcat cgagagccaa ccggcgcccg tagcgcttgg tcagcgcctg tgtttccagt
     9421 gcgagtgtca ttcgtttcct ttcagagcct cgcgcgcggc gtcgagcatg gtcgcgtccc
     9481 ggtagaggcc gtgggtgtag agctcgaggg agggcagcgt catccgccgg atcatcgtgg
     9541 cggtgagccc gggctcgccg agcatgcgcg cgaactggcg ctggagcacg atcggcatga
     9601 cgccgtgcag ggtcacgagc accgcccgca tgtacgggtc actcgtggac tgcatcacgc
     9661 cgttggcgac gccctcctcg agcatggtct cggtgagttt cacgaggtcg tcgagcagac
     9721 ggtcgccggc ctcggagccg tcgagcagca tgcgtccgag gtagtcgagc gtggggcggt
     9781 actgatcgag atcgctgagc atcacctgca tgctcgcgga gaggttgccc tcgtcgaccc
     9841 cgcccttgtt cttcatgagt tcagccacga tccacgcatc gcattctgcg cgcaacttct
     9901 ccttcgagcc gaagtggtga atcaccagcc cggcgctcac cccggcctcc tccgcgatgg
     9961 aacgaacgga tgtcgcgacg aagccgtcgc gcgcgaacag ggtgatcgcg gcatcccgaa
    10021 tgcgggagcg ggtggtcgca tcctcgacgg gagttgaacg catgttcagt atactaaacg
    10081 gtcgttcaat cgagagtcaa gctcacgggt gcaatgccgc cggtaggctt gcagggtgag
    10141 tgacatcgag atcggccgtg ccaagcgcgc gcgtcgcgtg tacgccttcg acgacatcgc
    10201 gatcgtgccc tcgcgccgca ctcgcgaccc gcaggatgtc tcggtgagct ggaccatcga
    10261 cgcctacacg ttcgacattc cggtactggc agcaccgatg gactcggtgg tcagccccag
    10321 caccgcgatc gcgatcggca agctcggcgg actcggcgtg ctcgacctcg agggcgtgtg
    10381 gacgcgctac gacgaccccg aaccggtgct cgcggagatc cggtcgctgc ccgcggctac
    10441 ggccaccaag cgcatgcagg agatctactc ggagcccgtg aagcccgagc tggtgactgc
    10501 gcgcctggcc gagatccgcg cggccggtgt acccgtggcc ggtgccctca gcccgcagcg
    10561 cacgcaggag ctctacaaga ccgtcgtcga cgccggggtc gacctgttcg tcatccgcgg
    10621 cacgacggtg agcgccgagc acgtctcgaa gaccgtcgag ccgctcaacc tcaagaagtt
    10681 catctacgag ctcgacgtgc ccgtgatcgt gggcggcgcg gccacctaca cggcggcgct
    10741 ccacctcatg cgcacgggcg ctgcgggagt gctggtcggc ttcggcggcg gtgccgcctc
    10801 gacgaccagg gcgagcctcg gcatccacgc gcccatggcg acagcggtgg cggatgtcgc
    10861 gggcgctcgc cgtgactaca tggacgagtc gggcggccgc tacgtgcacg tcatcgccga
    10921 cggcggactc ggcacgagcg gcgacatcgt gaaggcgatc tcggtgggtg ccgacgccgt
    10981 gatgctcgga agcacgctcg cgcgggccac cgatgctccc ggcgggggct ggcactgggg
    11041 cgccgaggca caccaccccg agctgccccg cggcaatcgc gtcgaggtcg gcagcctcgc
    11101 gccgctcgcc gagatcctct acgggccctc gacggcggcc gatgggcggg tcaacctcat
    11161 cggggcactg cgacggtcga tggcgaccac cgggtactcc gacctcaagg aattccagcg
    11221 cgtcgaagtc gtcgtagctc cctaccacgc gggataggtc atgccgaagt cagtcacacg
    11281 ctcgtccaag ctcggcgccg ccgagcgcgc cgccgcgatc gaggtgctga aggccaagga
    11341 actcgacatc ctcgtcatcg gcggcggtat cgtcggcacc ggcagcgcac tggatgccgc
    11401 gacccgcggt ctgcgcgtcg gaatcgtcga ggcgcgcgac tgggcctcgg gcacctcgag
    11461 ccgttcgtcg aagctcgtgc acggcggcat ccgctatctc gaacagctcg acttccggct
    11521 cgtgcgagag gcgctgatcg agcgcgggct gctcctgcag cgcatcgcac cgcacctggt
    11581 gaagcccgtg cgtttcctgt acccgttgac caagccggtg ttcgagcgct tctacatcgg
    11641 agcaggaatg ctcctctacg acctcttcag ctacaccggc ggtcgcccgc ccggagtgcc
    11701 ccaccaccgt cacctcaccc gccgtcaggt gctgcgcgcc atgccgagcc tcaaggccaa
    11761 cgccttcacc ggcgggctga cctactacga cgctcaggtc gacgacgccc gctacgtggc
    11821 caacctggcc cgcacggcga gcttctacgg cgcgcacgtg gcgagccggg tgcgcgtgga
    11881 gggcttcatc aaggtcggcg agcgtgtcgt gggtgtccag gcgcacgacc tcgagaccgg
    11941 cgagaagttc gagatcaggg cgcgccaggt cgtcaacgcg accggcgtgt ggaccgacga
    12001 cacgcaggcc atggtcggcg agcgtggcca gttcaaggtg cgcgcctcca agggtgtgca
    12061 cctcgtggtg ccgcgggacc ggttccagtc gaagctcggc ctgctgctgc gcaccgagaa
    12121 gagcgtgctg ttcgtcattc cgtggggtcg gcactggctc atcggcacga ccgacaccga
    12181 ctgggatctc gacaaggcgc acccggccgc caccgccgcc gacatcgact acctgctcga
    12241 gcacgtcaac aaggtgctgg ccgttccgct cacccgcgac gacgtggagg gcgtctacgc
    12301 cggactgcgg cccctgctcg cgggcgagtc cgaccagacc tccaagctca gccgcgagca
    12361 catcgtcgcg cacagcgtgc ccggactcgt cgttgtcgcg ggcggcaagt ggacgaccta
    12421 ccgcatcatg gccaaggacg ccatcgacga ggccgtcgcg gccctcgacg gccggatctc
    12481 cgagtcgatc accgaagaca tcgcgctgct gggcgccgag ggttaccacg cggcgtggaa
    12541 caagcgcggc aagctcgccc gggcgttcgg cgtgcacaag gtgcgcatcg agcacctgct
    12601 gaaccgctac ggcgtgctca ccgacgagct gctcgacctg atccgcgagc ggcccgagct
    12661 gcaggagccg ctgcccggcg ccgacgacta catcgccgcc gaggtcgtgt acgcggcaac
    12721 gcacgaggga gctctgcacc tcgaagacgt gctggcccgc cgcacccgca tctccatcga
    12781 ggcgtgggac cgcggggtct ctgccgctcc ggtcgtcgcc gagctgatgg cggccgagct
    12841 agggtgggac gccgcgcgca ccaagcgtga ggtgaactac tacctgaagc gagtgatggc
    12901 tgagcgcgag agccagctgc agccagacga cgcgtcggcg gatcgcatcc gtctcgaggc
    12961 accggatatc gtcgccagct agcgtccaga cggccccggg tagactggat gctcgcccag
    13021 aacgacgaaa gggacgtatc tcatggttga cgttcgacgg gtcaagcttc ccggggtggg
    13081 tgtacttcac accttcgtga cggatgacgg tggcaaggtc ggcgtgatca ctcaccgttc
    13141 cggccacagc gacctcatca ccttctccga cgccgaggac ggctccgacg tcagcaaggt
    13201 ctccctgcga ctcgacgagg acgaagccca cacgctcgcc gagctcctcg gtggcacgcg
    13261 catcaccgag tccctcgcga gcctcgacgc gattccgggc ctgtcgatcg actggttcac
    13321 cgtcgactac gaggatcaca tcgccggcaa gaaactcggc gggctcgcca gccggggcgt
    13381 cgtcggcctg accgtggtcg ccgtcgtgcg cggggactcg gccaatccgg cccccgccga
    13441 cgatttcacc gtctttccgg gggacaccct ggttgtcgcg ggaacgcccg agaaggtcgc
    13501 caaggccttc gcgttctacc gcacgggcga gatgaagtcc aaggtgtccg ccgactctcc
    13561 gcccggaggt tagtcgatgc aagggcacct cggcgaagag ctcctcgttc tcggcgttct
    13621 gcttgtcatc gcatacgtgc tgggcagact gggcaagctc atcggcctgc ccgccatccc
    13681 ggtctacatg ttggtggggc tcatggcgag cccgcacacg ggctggttcc cgctcaactt
    13741 cgagtcgggc aacgtcgaac tgatcgcgat cttcgggctg atcctgctcc tgttcaacct
    13801 ggggctcgag ttcgaccagg acgagttctt cggcaatgcc ggacgcctgc tcgtctccgg
    13861 tggaacgtac atcctcgtca acatgggtgt cggcttcgcg ttcggcttca tggtcggctg
    13921 gggcacccgt gaggcgctga tcatcgcggg catgacggcg acctcgtcga gcgcgatcgt
    13981 caccaagctg ctcatcgagc tcaaccgtct cgccaacaag gagacgccga tgatcctcgg
    14041 cgtcaccgtc gtcgaggaca tcttcatcgc catctacctc gcaatcgtcg gcgtggtgct
    14101 gagcggcgag accgaggtgt ggccggtcgt gctgaagctg ggcatcgcat tcctgttcct
    14161 cgtggccatg ttcacgatcg cccgctgggg cggtaggtgg gtgtcgaagc tgttccgcac
    14221 caaagacgac gagctgttca ccatcctgtt cttcggcctc gcggtcgcct tcggcggcat
    14281 cggcgagctt ctcggcgtga cggatgcgat cggtgcgttc ctcatcggcc tcgtgctcgg
    14341 ggcgaccaag taccgcgccc gcatcgaaca gttctctctg cccctgcgcg acgtgttcgg
    14401 agcgttcttc ttcctgaact tcgggttggt gctcgacatc ggcaccttcg gagaggtgct
    14461 ggcgcccgtc gccggagcgg tcgtgctcac gatcgtgctc aacgtcgtgg caggccagtt
    14521 cgtcgcctgg ctcaacaagc tggggccgca ggcgggcatc aacgcgacgg tcatcctgca
    14581 gaatcgcggg gagttcgccc tgattctcgc aaccctctca ctgtcggccg gtctcgacga
    14641 acggatcgtg cccttcgcgg gactctacgt gctcaccatg tcgatcatcg ggccgatcct
    14701 cgccgtcaac tccgaacgga tcggtgccgt gatcctcggc accaagcgca agaaggccgt
    14761 cgttgccccc gagcgcgacc gcatcaggga ggagggcatc gccctcgtcg aggccgcaac
    14821 ggccggccag gaggcgtccc agcccagtga cctcgacgac gccatcgcgc tgcacgtgcc
    14881 cgagatggcg gccgccgagc cccgtgaccc ggtcgcagag caggccgcca tgcagtccga
    14941 tgaggtcgcc gagcgcaaga aggatcccga gtattgagta caacggtgtg gcaggtcgcg
    15001 aggcgaccac gatggatcgc ggcgctcgtg ctggcgctcg gcgtcgcatc cgctttcgcg
    15061 gcgctcgggc agtggcagtt ggctcgcagc gtcgagggtg gccaggtcac cgaggagcag
    15121 accgagtcga gcgttccgct gacctcgatc gcggtgcccc agaagccgat gcccagtgac
    15181 accatcggcc agctcgtgac cgtgggcgac gtcacctggg tggcggggga ctggatcgtg
    15241 ctatccgatc gcagcaatca cgccgagccg gggtactggg tggttgcgca cgcggtcacc
    15301 cagaccggcg ccagcctcgc cgtcgcgctc gggtggactg ccagtgcgga tgccgcggca
    15361 tccgccatca cgaccctcga ggccaccccg cccgcggcgt cgctctccgg ccgttacctg
    15421 cccaccgagt caccccagca gagcgacttc gaggcgggaa agcacagcgc cctcgccgtc
    15481 tccgagctcg tgaacctgtg ggcagacgcc cccgacgggg tgtactccgg gtacgtgatc
    15541 tccgatcagg cgcccgccgg cctggagacg atcgacgcgc cgccgcccaa caacgaggtc
    15601 tctctcaacc tgctcaacat cttctacgcg atcgagtggg tgatcttcgc agggttcgca
    15661 atcttcctct ggtaccggct ggtcaaggat gcctgggagg aggagaacct tccgcaggag
    15721 agcccggctg agccgagccc tcaggagccg accctaaact agaggcatgc ccctcggacc
    15781 ccggccagat gacatccctc gcatccggcg cacggtctcc gtctacaagg tctcctcgat
    15841 catcaccggg tcgttcctgc tgctcctcgt gctcatgatg gtgctgcgct acggcttcgg
    15901 agtcgacatc gagctcctcg gcccgtacgg attcctcgcc ctcaccccga aggagttgat
    15961 cgcggggatc aacctctcga cggtcatcct catcgtgcac ggctggctgt acgtgctgta
    16021 cctcgcctgc gacttcctgc tgtggcgcta catccgctgg tcgttcgcgc ggttcctgtt
    16081 catcgcgctc ggcggcatca tcccgctgct ctcgttcttc ctcgagcggc aggtgccgag
    16141 gcaggccgag gcggccatcg cgtccatcga gaaggcgacc gcatgaccac agttcccgct
    16201 tccagcaccg aggcccgccc ggtactcgtc gtcgacttcg gcgcgcagta cgcgcagctg
    16261 atcgcgcgcc gggtgcgcga ggcgaacgtg tacagcgaga tcgttccgca caccatcacg
    16321 gcggccgagg tcgcagccaa gcaccccgtc ggcatcgtcc tcagcggcgg cccgtcgagc
    16381 gtgtacgagc cgggctcgcc cgacctcgat ccgggcatcc tcgagctggg catcccggtg
    16441 ctcggaatct gctacggctt ccaggtgatg gcacgccagc tcggcggcga ggtggccaac
    16501 accgggctgc gcgagtacgg cgccaccgac atgacnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnntcggc ggcgaggtgg ccaacaccgg gctgcgcgag tacggcgcca
    16681 ccgacatgac cgtgaccggt ggtggcggcg cgctgctgtc gggccagccc gaggcgcaga
    16741 cggtgtggat gagtcacggc gactcggtgt cccgggcccc cgaggggttc gaggtactgg
    16801 cgtcgagcga gtccacgccg gtcgccgcgt tcgcgtcgga tgagcgtcgc ctgtacgggg
    16861 tgcagtggca tcccgaggtc aagcactcgc agttcggcca gcgggtgctc gagaacttcc
    16921 tgcacactgc ggcaggaatc cccgctgact ggaacagcgg caacgtgatc gccgagcagg
    16981 tggcgcgcat ccgcgagcag gtcggctcgg cgaaggtcat ctgcggtctc tcgggcggcg
    17041 tcgactccgc cgtcgccgcc gcgatcgtgc acaaggccgt cggcgaccag ctgacctgca
    17101 tcttcgtcga ccacgggctg ctgcgccagg acgagcgccg ccaggtcgaa gaggactacg
    17161 tcgcctccac cggtgtgcgc ctcgtcaccg tggatgccgt cgaccagttc ctgaatgaac
    17221 ttgccggggt caccgacccg gagacgaagc gcaagatcat cggccgggag ttcatccggt
    17281 cgttcgaggc ggcggccgcg gatctggtcg cggaagcggc atccgacggc gagcccatca
    17341 agttcctcgt acagggcacg ctctaccccg acgtcgtcga gtcgggcggc ggaaccggca
    17401 cggccaacat caagagccac cacaacgtgg gcggtctgcc ggaggacctg cagttcgagc
    17461 tcgtcgagcc gctgcgcacc ctgttcaagg acgaggtgcg ggccatcggc cgcgagctgg
    17521 gcctgcccga ggtgatcgtg tcgcggcagc cgtttccggg gccagggctg ggcatccgca
    17581 tcgtcggcga ggtcacccgc gaccggctcg aacttctgcg cgcggctgac gcgatcgtgc
    17641 gcgccgagct gacggctgcg gggcaggatg ccgcgatctg gcagtgcccc gtcgtgctcc
    17701 tggccgacgt gcgctccgtg ggcgtgcagg gtgatgggcg cacgtacggc cacccgatcg
    17761 tgctgcgtcc ggtctcgagc gaggacgcga tgacggccga ctggacgcgc gtgccctacg
    17821 aggtgctcgc gaagatctcc aaccgcatca ccaacgaggt gcccggcgtc aaccgcgtgg
    17881 tgctcgacgt gacgtcgaag ccgccgggaa ctatcgagtg ggaatgacgc gcgagggccg
    17941 ctagctcctc tcgggcgggt gttgtggaca cgcgcgcatc ctgtggcacc gcgaaaagac
    18001 cacaacagcc gcgcgaagtg cccgctggtg aggcatgctg gacacgtgaa cgacgcccgt
    18061 ccgcctgcga tcgtgtcgct cgagcgactc cggtcgtcgt tggccgaccc cgggctctcc
    18121 ctgccgctcg agggggcagc aacgcaacgg gatgccgcgg catccgccgt gcgtcagatc
    18181 gacgactaca tccgtccccg cctggccgac ctcgatgcgc cgctgctggc ggtggtgggc
    18241 ggatcgaccg gctcgggtaa gtccacgatc gtcaacgcga tcatcggtac gcccaccacc
    18301 cgggctggcg tcatccggcc gacgacgcgc cagccgatcc tcgtgcacgc gcccgtcgac
    18361 gcggggtggt tcgcgtcgga tcgcatcctg cccgggctcg cgcgcgtgcg cggccgcatc
    18421 accgagccgg gcacgcccgc gtcgtccgcg ggggacgtgc cgacggcatc ccgaatgaac
    18481 gagctcatgc ttctggccag tgaccaggtg ccggcgtcgc tcgcgctcgt cgacgcaccc
    18541 gacatcgact ccgtggccga cgagaatcga gcgctcgcca accagctgct cgcggcggcc
    18601 gacctctggc tgttcgtcac cacggccaac cggtacgccg atgctgtgcc gtgggcgctg
    18661 ctcgacggcg cggcatcccg ggccatcacc gtcgcggtcg tgctcaaccg cgtgccgccc
    18721 ggtgccgcgg atgaggtgct gcccgacctg aagcagatgc tgagctcccg cggcctgccc
    18781 gacgcacccg tgttcgtgat cgaggagcag ccgctcgacg agctcgggat gctgcccgcc
    18841 gagtccgtcg ccggcccgcg cacctggctc acgggtatcg ccgccgaccg agtcgagcgc
    18901 gcccgcatcg ccgcccgcac cctgcgcggt gcgatcgtcg acctcgacat ccgtgtcgcc
    18961 tcgatcgcta ccgcccgaca gcagcagctc gactggctcg acggcatcgc caagtcggtg
    19021 gccctcgact acgacaacgc ggccgacgcc gtcgacgacg ccacccgcga cggtgccctg
    19081 ctgcgcggcg aggtgctcgc ccgctggcag gacttcgtcg gcacctccga cctgttccgc
    19141 agcgtcgagc gctggttctc ccgggctcgg gatgccgcga ccgccttcgt catgggcaag
    19201 ccgcaacccg tgatcgacgt cgaggtgcgc atcgagcacg gcctgcacgc ggtgatcgtc
    19261 gaccaggccg ggcgagcggc ggcggcatcc tgggcggggc tgcagcgcac cgaggctggt
    19321 cgcgcgatag cgcaggcgca cccggggctc ggcacggaga gccccgagtt ctcggagaag
    19381 gcggccgcga tgattcgcga gtggcagggc gcgctgctgc ggctcatcca ggacaatgcg
    19441 ggctccaagc gcatccgagc gcgcgtgctc tccgcggggc tcaacgtcgt gaccgtcgcg
    19501 ctgatgattg tcgtgttcgc ctccaccggc gggctcaccg gcggcgagat cgtgatcgcg
    19561 ggcggctcgg ccgtgctcgg ccagaagctg ctcgagacga tcttcggcga ggatgctgtg
    19621 cgccgcctcg cgggggaggc gcgcaaggat ctcaccaaac gggttggagc cctcttcgag
    19681 agggaggccg cccgggtggc cgccgccctc gaggccgcgc ggttcggctc gtcgcccgac
    19741 tcgctgcgcc gcgagtcagc ggccctgctc gacgacgtgc gccgggccgc cgacggaggc
    19801 acagcgtgaa ggtgacgctc gaccagcgtc tcgccgccct gcgcaccctg cgggagaccg
    19861 gcgccgggcg catcccggat gccctcgccc gcgacctcga cgccgtgctc acccactccg
    19921 gcgaacgacg ccagcgctcg cccgaacaca ccgtggtggg catcttcggc gccacgggca
    19981 gcggaaagtc cagcctcgtg aatgcactcg tgggggcgga cgttgcgcgc acgcacgtgc
    20041 ggcggccgac gacatccgag gctcatgccg tcgtctggga cccggatggc gccgtcgacc
    20101 tgctcgactg gctgcaggtg cgcgagcgca ctgtgcgcga tggccccatc gacgcgcgcg
    20161 cgggcaagct cgtgctgctc gacctgcctg acttcgactc ggtggagcga gccaaccgcg
    20221 agatcgccga acgcctcgcc gcccaggtgg acgcgctcgt gtgggtcgtc gacccgcaga
    20281 agtacgccga cgaggtgctg cacgcgcagt tcatcgcgcc gcacgcgaag cactccgcgg
    20341 tgaccctgat cgtgctcaac cagatcgacc tgctgcccgc cgctgacgtt ccgcacgtcg
    20401 tcgactctct gcgcggcatc gtcgagcgcg acggaatacc gaaggcgcgg gtgctctcgg
    20461 tgagcgctcg cacgggcgag ggcatcgacg cgctgcgcaa ggctctcggc gacctggccg
    20521 cggccaccga ggtgcgcgag gcccggctcg ccgccgacgt gagcacggtc gtcgcccgcg
    20581 tgcctgcggc cggaaccgtc acgcgtccgg gcaagggcgc catcgacaca ctcgtgaccg
    20641 acctcggcac tgctgccggc gcggacgtgg tcgcccaggc cgtcgcccgc tcctaccgca
    20701 agcgcgccgg gcaggccacc ggctggccga tcgtctcctg gctgctgcgc ctgcgccccg
    20761 acccgctcgc ccgcctcggc ctcggcccga agcgccgcgg cgagaacccc gacctgcacc
    20821 gcaccagcat gcccgccctc gacgccggag cccgggcgcg cgtgtccttg tccgtgcgag
    20881 gcttcgcgga tgccgcggcc gcaggtctga ccgacgcgtg gcgcaccggc atccgcacca
    20941 cagccgaccg agccatcgcc gacctaccgg accagctcga cctcgccatc gcccgcacca
    21001 acatcccagc caagggctcg tggtggtggg tgatcttcag cgtgctgcag tgggtgagta
    21061 tcgtggccgc cctcagcgga atcgtctggc tgctcggcgc cgcgttcctg cccaccttcg
    21121 ggctgcccgc attccaggtg cccaaggtcg aggggtggtc cgtgccgacg ctgctcatcg
    21181 ccgggggtgt ggccctcggc atcctgctcg ggatgctcgg ggccctgctc gctgcgatcg
    21241 ccgcgtcatc ccgccgccga cgcacccgaa aactgctcac cgcgtcggtg cgcacggtgg
    21301 tgcaggacac cgcggtcgcc ccactcgtgg ccgacctcga tcgtgcgtcc gcctacaccg
    21361 ccgccctcgc ccaggctggt tgagtagcgg cgaagccgcg tatcgaaacc accccgccgg
    21421 ttgagtagcc cgcgtagcgg gcgtatcgaa accaagcccc gcgtgccgcg ctccccgcaa
    21481 cgcccatcga atttcagctc acacatcggt gcgcttcgac agcccctgcg gggccatcga
    21541 accaccccga aactcgcaag aagtccgacg gtcgttgcgg tcgcggggta tcgggtctgc
    21601 tcgagctagg tcaggagcct tacgagcgag accaccgcga gagcaacccc gcagaggcag
    21661 aacacggaac cgccaatccc gatcacgact ggatgctgga acttcacccc gcgccccttg
    21721 tcccactcca tccgctgggc gattgctgcc ctcgatatcc ggcgcctgaa gagtgcgaaa
    21781 gcgagccccc acagcgcgaa gcagccaccg atgaccaagc caacccacgg ctcctgctcg
    21841 aggatcatgc caagacccta gcgttgacag tgtcgttttg agcaggtagc gctgactggt
    21901 tccgcgtgca caaatgcacg gattcctgcc gacacgccgc tggcaggacg acatgagcgg
    21961 cgtgtcgggc caattctccg cagttgcgta cggcgaagcg ggcgcacccg cccactaccc
    22021 ctcgtgcccg gtgggcgcag cgcaccacgt gacatcccgc cgcacaaacg acgaaggccg
    22081 ccccgaggga cggccttcgc gatgagaagc agcgctagtt gcgcgcgatg gcgaacatgc
    22141 gcagcagctc caggtagagc cacacgacgg tcaccatgat gccgaaggcg ccggtccagc
    22201 cgtacttggc cggggccttg ttgttgactc cgcgctggat gaagtcgaag tcgagcacca
    22261 gcgagtaggc cgcgaggatc acgacgaaca ctccgaggat cacgccgagc gggattccga
    22321 agatctcgac gctgccgcgc agaccccacg ggttgccgcc ggcgccgccg aagatcatga
    22381 tgccgacgtt cacgagcgag aacacgaggt agccgaccat cgcgatgagg aacaccttgg
    22441 tggccttcgc ggatgcccgg atcttgccac tggcgaacag cgcaagcgtc acgcccacga
    22501 cgacgagcgt cgcgatgaca gcctgcacga cgacgcccgg ccacatggcc tcgtagaacg
    22561 cggagatgcc tccgatgaag acaccctcgg ccgcggcgta ggcgaggatc agcggagcgg
    22621 acggctcctt cttgaagatg ttgacgagcg ccagcacgaa ggcgacgatc gcggcaccga
    22681 tccagagcag cggaactgcg ggtgcggtga tccagccgat cgccgcgccg gccagcagca
    22741 ggatgaacga gatgaccgtc ttgacgatgg tgttctcgac ggtcatcgtc tcgccctcgg
    22801 cgggggccga ggagcggttg tagatctcgt cgagctgctg cgcgctgatg tcctgggcta
    22861 cggcgacgcc gccggcctgg gcgctgaacg cggggctgcg ggtgaacgcg gggttactgc
    22921 ctgccatggt tcctcttttc cggggattcc ttggctacaa atctaccggg gttttgctgg
    22981 gaaaaggcgg gtcgttgggt gtctctgcgg cggctgcgcc ctgccgcgcg atcaggcggg
    23041 cgagccaggc ggatgccacg aacacgacca tcgagatggc gagggcaagg ggcatcccgt
    23101 cgtactcctc gaagaactgc ggcacgatga agccgttggc gatggggaac agcaccgtga
    23161 tgtagctgcg cagcgggcga tggcgcacgc acagccacag caggtagggg gcggcgaggg
    23221 tccacaccga ctgcgggccg gtgagcagtg cgaccgcgaa gagcggggcg aggaaggcgg
    23281 cgagggtgcg gcgccatccg cgggcgggca tgatcgacca cccgatgacc agcgcgacga
    23341 tgccctggcc gaggaagctg acggcgtaga tgttgccggc ctgcatcgcg gtcgtgccgg
    23401 agatgatgag gatgaggccc cacaggtagc ggtcggtgta tcgaccccac ttgagcggga
    23461 tcagcgacgt cggctcgcgc cagactgcgg ggccccaggg tgccgcgggg ggctcagaac
    23521 cagacattga tggcgaacca ggcgatcacg atcgcggcca gcgagaaaca ggcgatagcg
    23581 aagacgaggg gagcgtaggt ggcgatcttc tcgcccacgg cagtctcggc acgctccggt
    23641 cgaacggctg ccgggatgac cggcgtgccg cacatgatgc agaacttcca ctcggggcgc
    23701 agctcggcct cgcaggaagt gcacgtggac atagtggaat gctagtcggg tgactgtcgc
    23761 acgagttccc ctcatcatcg ggcatcgcgg tgcgagcgga taccgtccgg agcacacccg
    23821 cgcagcctac gaactggcct tccggttggg tgccgacgcc gtcgaaccgg acatcgtggc
    23881 gacacgcgac ggcgtgctcg tgctgcgcca cgagaacgag atctccggta cgacggatgt
    23941 cgcctcccgt cccgagttcg ccgaccgtcg caccaccaag ctcatcgatg gcgcgcgcct
    24001 caccgggtgg ttcaccgagg acttcacgtg ggcggaactg tcgacgctgc gggcggtgga
    24061 gcgtctgccc caggtgcggc aggcatccgc gacgttcgac gggctcgagc cgatcctgcg
    24121 gttgcgcgat ctgctgggca tcatcgatga cgcgtccggc gaccggcagc gcatcatggt
    24181 cgccgagctc aagcacgcca cctacttcga gtcgatcggg ttgccgctcg acgaactgtt
    24241 cgcgtccgag gtgagcggtt gggcctccga cgagaacctc atcgtggagt gcttcgagca
    24301 gaccgtgttg ggcaaggtgc gggcgcgcgg cgtgcctgga cgcgtggtgt tcctggccga
    24361 ggcatccggg tcgccggctg atctggtcgc gcagttcggc tcgaaggcgc tgccgtactc
    24421 ggctcacctc acgaacgctg gcctcgcccg gctggcgacg gcggtcgacg gggtgagcgt
    24481 cgacaagcgc cttattctgt cgacggatgc cgccggcaca accaccacga ccgacttcgt
    24541 cgagcgcgct cacgccgcag gtctggaggt ctacacgtgg accctccggg cggagaacaa
    24601 gtttctcgcg aagggcatgc ggcgcggctc ctccgcgcgc gactacggcg actggctcga
    24661 cgagttccgg ctcatcctgg gcaccggcct cgacggcgtc ttcgccgacc agccggacct
    24721 cgtgcgcgag gcgctcgtga gcggcagcta gccgcgggag tcagccgcgg gagtcagcgg
    24781 cgggagccag cgtcatccgt cgcccgtaga atcggggacg atgacctacg tgctggaacc
    24841 gttcgatcag gccgatccgc ccggcgacgg acggcccggt cggctcgaca gcgccatcct
    24901 cgacgggctc aacccgcagc agcgcgaggc ggtcgagtac cgcggccagt ccctgctcat
    24961 cgtggcgggc gccggctccg gcaagacgag cgtgctgacc cggcgcatcg ccggtctcct
    25021 gcagacgcgc gaggcgtggc cgagtcagat tctggcgatc acgttcacga acaaggccgc
    25081 ggccgaaatg cgcgaacgcg tgaagtccct ggtcggggat gccgccgagg gcatgtggat
    25141 ctcgacgttc cactcggcgt gcgtgcgcat ccttcggcgg caggccgagg cgttcggcat
    25201 gaagcagagc ttcaccatct acgacaccca ggactcccgt gcgctcatga agcgcatcat
    25261 caaggagctg gatgccgaca ccctcggctt caccgcaggc ggtgccctca accgcatctc
    25321 gaagctcaag aacgagctca ccgacgtgga ggcgttcggc cgcaccgcga atctctccga
    25381 cccgaaggag gcggtgctgc tggagatctt ccggcagtac agcgcgagcc tcaagcgggc
    25441 gaacgccctc gacttcgacg acatcatcgg cgagaccgtg tacctgttcc gggccttccc
    25501 ggatgtcgcc gccctctacc agcgccgctt ccgccacgtg ctcgtcgacg agtaccagga
    25561 caccaaccac gcgcagtacg cgctgatcaa ggagttggtg aaggcagtca ggccgcagca
    25621 cgtgccgccg gacacgcgca tgccgctcgc ccccgacggc tcgattccac cggcatccct
    25681 cacggtggtc ggcgacagcg accagtcgat ctacgcgttc cgcggtgccg acatccgcaa
    25741 catcgtggag ttcgagcgcg actacccgaa ctcgaaggtg atcctgctcg agcagaacta
    25801 ccgctccacc cagaacatcc tcgacgcagc caacgcggtc atcagcaaca acttcgaccg
    25861 caaggcgaag aacctgttca cggtggtcgg cgagggcgag aagatcgtcg gctacacggg
    25921 ctactccggt cacgacgagg cccagttcgt cgccgacgag gtggaggtgc tgcgctcgca
    25981 ggggctcaac ctcaacgaca tcgcggtgtt cgttcgcacc aactcgcaga cccgcccgtt
    26041 ggaggagatc ttcatccgct cggcgctgcc gtaccgcatc cccggcggca ccaagttcta
    26101 cgagcgggcc gagatcaagg atgcgatggc ctacctcatc caggtcgcca accctgacga
    26161 cgacctggcc ctgcgccgca tcatgaacgt gcccaagcgg ggcatcggcc cggcgaccga
    26221 ggcggcgctt caggcctacg ccgaccgggt gggcgtgccg ctgcgcgagg ccctcgtgga
    26281 tgccccccag ctcggtctcg gaccgaaggt caccgcggcc atcgtcgatc tcggcacgat
    26341 cctcgacgag gtggcggcat ccgcggcgac cgcggctccc gccgacatcc tcaccgagct
    26401 gctcaaccgc accggcctgc accgcgccct gaaggccagc ggcgacccgc aggacgaagc
    26461 acgcgccgag aacctcgagg agctcgtgtc gcagaccaag gagttccgca ccgcgaaccc
    26521 cgagggcacc ctcgtcgact tcctcacggg catcgccctg ttcgctgcgg ccgacgagat
    26581 cgacgactcg ggcggctcga tctcgatcat gaccctgcac acggcgaagg gcctcgagta
    26641 cgacgcggtg ttcctcaccg ggctcgagga ggacctcctc ccgcaccgca tgtcgagcaa
    26701 cgagccgggc ggcctcgccg aggaacgacg gttgttctac gtgggcatca cgcgcgctcg
    26761 caagcgtctc tacctgtcgc tcgcggcatc ccgtgcgagc ttcggcgacg tgaacatcgc
    26821 gatgccgagc cgctacctgc aggagattcc gaccgagctg atcgagtggc ggcagtcgcc
    26881 gggcatggcg aactcgcgcg gcggcacgcg tccgcgcgca ctcaacgccc gcagcggcac
    26941 cggcttcagc tacggccagt cgttgccccc ggcgccgacc cgcgtcaaga ccgagtggac
    27001 cagtgccgtg accggcgcgg tgcgcgacaa cggcgacctg accctcgcgg tcggggaccg
    27061 catccggcac accgacttcg gtgacggcag cgtcacggcg gtcaccggcg agggcaccaa
    27121 gaaggttgcc gaggtgcagt tcgatgtggc cggccgcaag cggctgctca tcaagatcgc
    27181 gccgatcgag aagctgtgag agctcccgac gccgaacggc gagcgcgcgt agccgttccg
    27241 cgcctgcggg tgcccgacgc gcaggacgag ctggtcgcgg cgctgcgggt gatccgcgag
    27301 gagctggagc tgcccgagtc cttccccgcc gaggtggagg ctgaggcgac gcgagccgcc
    27361 gcccgagcca gccgcccgga gaacgatctg acgggcatcc cgttcatcac catcgacccg
    27421 gccagctcga ccgacctcga ccaggctctg cacctcgagc gcgacggcga cgggtaccgg
    27481 gtgcgctacg cgatcgccga cgttcccgcg ttcgtcgagc ccggcggcgc gatcgacacc
    27541 gaggccaggc tgcgcggcca gaccctgtac gcgcccgacg ggcgcatccc gctccacccc
    27601 acggtgctga gtgagggcgc cgcgtcgctg ctcgagggcg tcgaccggcc cgccttcgtc
    27661 tggaccttcg cactggatgc cacgggcacc gtcaccgcga ccaccctcga acgggcgctc
    27721 gtgcgcagcc gccgcaagtg cagctacatc gacgcgcagg ccgacatcga cgcgggcaca
    27781 gccgacgcat ccctggccct gctcccggtg atcggcgagc tgcggctcgc tctcgaacgc
    27841 gagcgcggcg gtgccagcct cgatcgaccg gaggacgaag tcgccgtcac cgacggccgc
    27901 tacgagctgg tgcgccgagc gccgctgccg atcgaatcgt acaacgctca gctctcgctc
    27961 atgaccggca tggctgcggc gcagttcatg ctcgacggcc gcgtcggcat cctgcgcacc
    28021 atgcccgccc ccgaccccga gacgatcgcg cgcttccgcg cccaggtgca ggccatcgga
    28081 tgcccgtggc ccgagcaaca gccttatggc gagtacctcc gtgccctcga ccgcgacgac
    28141 ccccgcggtc tcgccgtgct ccacgccgcc ggggcgctgt tccggggtgc ggggtatcgc
    28201 gccttcgacg gtgaggttcc ggaggacacg atccaggccg cgatcgccgc cccctacgct
    28261 cacgcgaccg caccactgcg ccgactcgtc gaccgcttcg tgctggtgac ctgcgaggcc
    28321 ctggccaacg gccgaccggt gccgggctgg gtgcgcgagg ctctacccac actgcccgcc
    28381 atcatgagcc ggtcggactc cctcgcctcc cgcctcgacc gcgcctcgat cgatgcgatc
    28441 gaggcggcgc ttctgcgcga ccgggtcggg gagacgttcg aggcgatcgt catcgcgacg
    28501 acagccgcag gcggaaccgt gcagttgagc gacccgttca tcacggccgc cgtcgagggc
    28561 gacgtgcgac ccggccagcg cgtgaaggcc acgctcgtga cggccgacat cgcgaccggt
    28621 gccacccggt tccgcgtcgc gggtctgggc gtcgtcgact agagcggcct agggctgcgt
    28681 cgcagccgga cgagctgacg cgcggcgcac cagcaccacg gccagcaggg acgtcgcgag
    28741 aatcagcagt gctgcggcat ccacaccgat ggcgacggac ggaccgaacg cgctcgcgag
    28801 caccccgatg agcacaccgc tgccgagccg cagacccatg ctcgtcatcg agtaggcgcc
    28861 gaggaaccgg ccgcgccggt cggcgggtgc accgagttgc acgaccgcct gcgaggtcga
    28921 ggccgacgtg aggttgccca ttcccgcgat cacgagcatg gccaccgaca gcaccaggtt
    28981 gtgcgacagc gcgaagacga tgaggctcgc ggcgaacacg atggtgctga cgatcgccag
    29041 gcccgccgtg gcccggatgc gcccaatcgc ctccagggag acccccgccg tcacggcacc
    29101 gaacgacatc gccgcgatca gtgccccgta gccgacgccc gacccggtcg ctccgagcag
    29161 ctcgccgaaa tcgggcagga gcggaagcag ggcggtaccg atgaacaggc cgaccgcgcc
    29221 ctgcaggatg aacacggtga gcaccgacgg gtagcgcggg atctcgccga gcacgccgaa
    29281 ggtctggcgc agtgtgaggc gcggccgcgg agtctgtccc gctcgggtgt ggccggtgta
    29341 cggcacgatg ccgagataga tgatgaacgg caggtagagg gcgatgttga ccaggagtcc
    29401 ccaggcgggt cccacggtga acagcagcgc ggcgccgatg agggggccca cgagcatccc
    29461 gaggttgagg cccgtggcca tgaggcgcac ggcactcggc aggtcttcgg ggccgacgat
    29521 gtcgtagagc atcatctgct cagctggccc ccacagcgcg ctggccagcc cgtgcaggct
    29581 cagcagcagc acggcgttcc atggctgaag cgttccggtg atgatgacga tcgcccagcc
    29641 gagtgacgcg agcatgaaca gtccctgcgc gatctgcacg atgcgccggc agtcgtagcg
    29701 gtcggcgagg ccgccgaaga tcacgccgaa cagcaggtgc ggcaaccagt ggctgatcac
    29761 cgcgaagcct gcgagcaggg gcgaatggaa cgcctgccag atcacccagt aggtgatggc
    29821 gtgctcgacg tggtctgcgc ccatgccgag tgaaccgccg gcgaggtagc ccttgtacca
    29881 gggcgctcgc agcgcggaga atctcaccgc cccagcctaa aggccttcag gtagtgcctc
    29941 tgcggcgcgt gccgcaccca gcgcggcaac cgcggataga cgaaccgcac aatgcgaacg
    30001 agccgctggt aacgccgctc gacgaacggc aggccgtagg cactgcgcag cggttggtcc
    30061 agcagtccgg ccgtcacggt gcgcacggtg ggcatcgcga gcctgagcca gagcggcccg
    30121 cgccggggat gcagcagctc gcgcgcgacg tgggcggcgg cggcatccac ggccagctcg
    30181 tcgacgcagc gcgaccagta ggcgtcgaag gccgcgcggt cggcgggcca cagcgcgcgc
    30241 ggaacgcc
//
LOCUS       DAOSOU010000003        25520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_1936_length_25520_cov_5.616768, whole genome shotgun sequence.
ACCESSION   DAOSOU010000003 DAOSOU010000000
VERSION     DAOSOU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 25520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25520
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_25520_cov_5.616768"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1591)
                     /locus_tag="PLW15_00310"
     CDS             complement(20..1591)
                     /locus_tag="PLW15_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32873.1"
                     /translation="MVAQFLRLKLTLLGNSFRRSPAQLVGMLIALTYGLGVAAFIVVG
                     LVSLRFASTDISRTVVIVFGSIVVLAFLLLPLAFGVDDTIDPRRFSLFGIETTRLSIA
                     LAISALVSIPSLLIVIFAVAQIVTWSSTGPAAGLAVLAAVLIVATCVLAARVSSAVAS
                     LVLATRRVRETTAVILVAVLALLAPLLAIMATIDWGSRGLPILRRIAAVLTWTPLGAA
                     WSIPADAADGRWGQVPLKILIAVAFLIALALVWRWLVGVMLTTPQREGMARSYTGLGW
                     FERVPTTPTGAVAARSLSYWSRDARYRVGLAVIPVVPIVMVGALLIAGVPIGLLAWLP
                     VPVMCLFLGWTVHNDVAHDSTAFWAHVSANLAGRADRWGRLVPALGIGVPLVLIGSVV
                     TSTVSGNWAALPGFIGLSASVLLFGLGVSSVISAAFPYPAVHPGDSPFAQPQAAGATG
                     SVVQSLSFTVTVLSAGPVAFLAFLGETVSPVWHFAALGAGLLIGVGALIGGVFWGGRL
                     VDRRAPELLAFTLQN"
     gene            complement(1567..>2156)
                     /locus_tag="PLW15_00315"
     CDS             complement(1567..>2156)
                     /locus_tag="PLW15_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT32874.1"
                     /translation="GTVTVHGSDVWSDPVVAKRNMGVLPDRLRLFDRLTGAQLLFYAG
                     ALRGMDGETVRARTADLAHAFGLEASLSRLVSDYSAGMTKKIALAAAMIHSPRLLVLD
                     EPFESVDPVSSATVTEILQKYVAAGGTVLLSSHSMDLVQRICDYVAVIVDGSVLADGT
                     MADVRNGVTLEERFVELAGGRETVEGLEWLHSFSD"
     assembly_gap    2157..2256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2257..2906)
                     /locus_tag="PLW15_00320"
     CDS             complement(<2257..2906)
                     /locus_tag="PLW15_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT32875.1"
                     /translation="MPPPAAVTEPDETTDGPTVIEAEPATSEATSSGVLGFTVPKLSI
                     PTIMLGRSKSQGAAPVEAPDEPTLVAEPSDPTPEPDGEPEAEPELEPEAEVEPEAELE
                     TEPEIEAATEPEAEPEHPADSVAVVTPATKSVARKKGKTVPPILRVDSLTKRFDDKLA
                     VNDVHLEIAPGAFYGIVGPNGAGKTTTLSMITGLLRPTAGTVTVHGSDVWSDPVVA"
     gene            3276..3968
                     /locus_tag="PLW15_00325"
     CDS             3276..3968
                     /locus_tag="PLW15_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOT32876.1"
                     /translation="MTTQIVILAAGMGSRLGRSLPKPLTELADGRTIMQQQFDNIRHA
                     FGNEAHVTIVVGYKLEHIIEAFPDASFVYNEQYDQTNTSKSLMRALQASTPGGVLWMN
                     GDVVFDPTALERAALMVARDQSFVSVNTAKVSDEEVKYTTDAEGFIKELSKTVTGGLG
                     EAVGINYISRNDKAVLLRQLKRVGDQDYFERGIELAIEQDGILIEPVDISDLYAVEVD
                     FAEDLERANLFV"
     gene            4010..4807
                     /locus_tag="PLW15_00330"
     CDS             4010..4807
                     /locus_tag="PLW15_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32877.1"
                     /translation="MTNSALGRPVWSPAARYRRSLWLLTVRDLRVRYSTSVLGYFWSI
                     LDPLVMAAIYWFVFTQVFDRTVGAQPYIVFLLTALLPWMWFNGATSDATRAYLREAKL
                     IRSTKIPRTIWVARLVLSKGIEFVASIPVVVIFAIATGATLQWEAALLPLALLLQAVL
                     TMGVGLIVAPLVVFFRDLERATKLALRFLFYASPIIYGLSDLPSGLHFWATFNPLAGI
                     FSLYRSAFFPEQLDWFAVGMAAIISAIIFCVGVLVFARTERAVLKEI"
     gene            4811..5557
                     /locus_tag="PLW15_00335"
     CDS             4811..5557
                     /locus_tag="PLW15_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT32878.1"
                     /translation="MTESENVVIRVDDAGIRFRRNRRARRSFKDLFAGRKRRTRPGEF
                     WALRHVSFAVHSGEAIGVVGRNGQGKSTLLKLVAEVILPDEGRVQVAEGVAPLIEITG
                     GFVDDLSVRDNVFLTAGLHGMTRAEISERFDEIIDFAEIRDFLDTPYKHLSSGMKVRI
                     AFSVISRLEEPIILVDEVLAVGDKGFREKCYRRIEELLAGGRTLFFVSHNEKDLRRFC
                     TRGLYLDAGSLVLDGPIGDVLERYNADYAE"
     gene            5600..6442
                     /locus_tag="PLW15_00340"
     CDS             5600..6442
                     /locus_tag="PLW15_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32879.1"
                     /translation="MAKTQSVSVSNPMMVRGIDRLLTVQRPVVLAHIRSIRRGRPNAS
                     PAEIIRTLERRYLTAVTTGGAAVGATAVVPAIGTGTSLALSAVETAGFLEASALFAQS
                     ITEVHGIVVDDPDRARALVMTMVLGTAGTELVGQLAGQVTGSAPARTAFWGETITKNL
                     PRAVMGPIADRIKRTFVKKFAVAQGTNVVGRLIPFGIGAAIGGGGNHLLGRQIVRTAR
                     EGFGPAPDTFPSWLEPVIREPKAPKAPKPAKDARPQGQRRELRIPLPSLPRRAEKWVE
                     SPEE"
     gene            complement(6446..7741)
                     /locus_tag="PLW15_00345"
     CDS             complement(6446..7741)
                     /locus_tag="PLW15_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOT32880.1"
                     /translation="MPLLHDIRTARNVLRGILRSRRTRAQVARREPDQKRPAPGTIKI
                     AVYFADSKVNLYQIRQWYAPLAEISQTWPVAIISRSPSAVLELWNESPVPALYLRRVT
                     DLEEFVHTQDVRIVFYVNQNAKNFQMFRYGRMWHVFINHGESDKMYMTTNQFKAYDFS
                     FVAGDAAIDRLSHKLWDFDLATRAIAIGRPQADHFAGELPYTADDRTVVLYAPTWEGD
                     RDAAAYGSIASHGVTLVRALLATGRHRVIYRPHPRSGVVDSEYGAANREIIDAIARAN
                     VADPAAQHIFDDGPSLGWQLAAADVAITDISAMVYDRLATGKPLIVTRPVSASAEVDE
                     TGYLGACEWLTADAAGDIVAIADAVQFDAEAQKRLKFWVERHFGDTTPGVATARFHEA
                     IERLMALWEVNARIHEGDSVGSEGDPFDDDDDEDALPSD"
     gene            complement(7778..8644)
                     /locus_tag="PLW15_00350"
     CDS             complement(7778..8644)
                     /locus_tag="PLW15_00350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT32881.1"
                     /translation="MIPTVGVVSLSQGTRPDDLARGLASVLAQTGVTLDVIVVGNAWT
                     PTGLPRGVRSLALRENVGIPAGRNRGAAEVSGDYIFFLDDDASIPSPTFLVDAIALLE
                     ADPSIGLLQPRVVDPSGVENPRRWIPRIRKGDPEHSSNVFSVWEGATLLPRAVFDATG
                     GWADPFFYAHEGIELAWRVWDTGHRVLYAGDLVANHPAIEPTRHSYYYRLNARNRVWL
                     AKRNLPWILVPIYVGSWTGIQLLRWARRPQALKAWFGGWVAGWRENPGGRRGIRWVTV
                     WRMTRAGRPPLV"
     gene            complement(8641..9450)
                     /locus_tag="PLW15_00355"
     CDS             complement(8641..9450)
                     /locus_tag="PLW15_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT32882.1"
                     /translation="MSVDAHPTRPTSIAQLREVSQPPEVRLRANAEHWTASLYLRDLS
                     PYLTWLLLRTSISANGVTGLMILVGWSTAAALLIPGIGGAALALVLGQLQMLVDCCDG
                     EVARWRNTRSPAGVFLDKVGHYTTEALIPIALGIRAAGWPLEFPADFAWTSAGLALAL
                     LIVLNKALNDMVHVARANAGLEKLSDRKEEAVPSGRVIARLRRAARFVPFHRLYHSVE
                     LTIIIFAASVVGLFLPPLVAERWVLLILLPLALLALVGHFVAIMASKRVRS"
     gene            complement(9453..10478)
                     /locus_tag="PLW15_00360"
     CDS             complement(9453..10478)
                     /locus_tag="PLW15_00360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT32883.1"
                     /translation="MAAKSDALPGVSYVMPVLNEVQHIEAAINSLTAQDYAGDFEIIL
                     ALGPSVDGTNDIVSAMAAADPRIRRIDNELGSTPGGLNVAIRASVNPVVIRVDAHSVL
                     PPDYTRVAVRTLLETGADNVGGIMKAVGVTPFEKAVAHAYGSPEGLGGTQHHVGGKAG
                     PADTAYLGVFRRERLLEVGLFDEDIKRGQDWELNRRLRATGGTVWFTPELEVVYRPRS
                     SLRKLIRQFVATGIWRGELARRFGTANSVRYFIPPLAVIGIFGGLILGVLGMVLSTPW
                     MLLGFAVPGAYAVLVIAASVIAAREGFTTGLWYLVVLPCIHFGWGTGFVLGFLKITRN
                     ITAHTGR"
     gene            complement(10601..11917)
                     /locus_tag="PLW15_00365"
     CDS             complement(10601..11917)
                     /locus_tag="PLW15_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT32884.1"
                     /translation="MTTSPEKNESAPVDGAATATPIDPAQHPALAKQRRTIAPVGVIA
                     ALAVGALVGGVSGAGVTLWATSANSGAPSVTTSSPTTITVNDPANATQITAVAAKASP
                     SVVTISVSSPNASGTGSGVIIDTEGHVLTNTHVVTLEGAAADPTIQVQTNDGRLISAK
                     IVGIDPISDLAVIQLADTSNLQPAEFADSSKLNVGDGAIAIGAPLGLAGTVTDGIVSA
                     LNRSITIASSALPDTSTNNDQAPDTQSPFDFWNFDLPGQNQSNTPTSSSQTISLSVIQ
                     TDAAINPGNSGGALLDSEGRVIGINVAIASAGSSSGGQSGSIGVGFAIPANLAQRIAG
                     ELIATGKATHGLLGASVADVTEDTTQTNSTVVGASIVELTSGGAASAAGLAVGDIITG
                     INGLPVTGKTDLTAQVRALAAGSKATLNYVRDGKPATVDVTLGSLS"
     gene            12047..13279
                     /locus_tag="PLW15_00370"
     CDS             12047..13279
                     /locus_tag="PLW15_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT32885.1"
                     /translation="MSVSGSWERTARGAMLLGPGGELSPTIFAEMSALATSTGAINLG
                     QGFPDEDGPAEVLEAARAAISAGVNQYPPGLGMPVLREAIAAHQKRFYGIDVDAATQV
                     LVTAGATEAIAATLLALTDAGDEVVTFEPFYDEYGAITALGRATQVTVPLRAPHFLPD
                     LDELRAAVNDRTRVILVNSPHNPTGVVFPREILELIVELAHRHDAIIVTDEVYEHLTF
                     GVEHVPVASLPGAFERTVTISSGGKTFSTTGWKIGWAIAPAELIQAIAAVKQYLTYVN
                     GAPFQPAIAVGLGLSDEFFAGAAATLRAKRDILSAGLASAGFTVGTPDAGYFVIADAA
                     PLGVTDAAEFCRALPSLAGVVGVPVTAFVRPANRAQYSSLIRFAFCKRAELLEDAAAR
                     LAGMTVVSDAGPNLRGNA"
     gene            complement(13244..15013)
                     /locus_tag="PLW15_00375"
     CDS             complement(13244..15013)
                     /locus_tag="PLW15_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT32886.1"
                     /translation="MTAMDRVRVWTHNILNRPSPIVKQSATLGAVVAAVLIIALVPDV
                     SMNYPALAWTGVAVVGFATVLAVVLSRVHEWHRFALLVPVIDIFAIGAFRGGTGGVMS
                     PFTALIVLPVVWLASGNGRRYILYSGVGTFLALLIPVFLGLTAPSAPSEWVRGTYTPL
                     VLTIAAGIVNELARQGREQLRSIRGLVDEKERMLQRTVEFAAQLQASESKYRAADRMF
                     RGVWAAVTEQSVIGTDATGLIDAWNPGATKLLGPDRDDAEDKMHIFDFHLPEELEDRA
                     RELNYPPGETVLNPGFSALVESARLGKSETREWTYVRPDGTHVPVQLSVTKRVDEDGD
                     TVGYLFVASDVTQAREVSRLKDEFVGLISHELRTPLSSILGYLELLRDDEDQPLSAEQ
                     LQYLSVAERNAHRLLRLVGDLLFTAQVESGKFPLDARDIELEAIITASIETARPAADN
                     AGIEVLNVLPDGPVMVHGDSTRLGQAVDNLVSNALKFTPRGGKVIVELSTSGSNAVIT
                     VRDTGMGIPAEELDMLFSRFFRATTATRNAVPGVGLGLTITKAIITAHGGAMGVSSEE
                     GVGTEFSVTLPLSRVAAEVRPSV"
     gene            complement(15010..15384)
                     /locus_tag="PLW15_00380"
     CDS             complement(15010..15384)
                     /locus_tag="PLW15_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT32887.1"
                     /translation="MTHTVVIADDDADIRQLVTIAVKKAGLTVAAVAADGAEALEAIR
                     THHPDLAILDIAMPEMSGVDVCRAVRADSSIGDVRIVLLSASVEEQAIEVGINAGADY
                     FFMKPFSPRDLVAWLAVGKEAR"
     gene            complement(15381..15716)
                     /locus_tag="PLW15_00385"
     CDS             complement(15381..15716)
                     /locus_tag="PLW15_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT32888.1"
                     /translation="MEDSADQAGLLRQYFEKAGCVVHVVGTGEEALAAAADLPPDLAV
                     IDLMLPGMGGWELITRFREVVPECAIVVASVLDASDFPVADAILPKPFTGAQVRQVLA
                     DLTTLGRIP"
     gene            complement(15746..15961)
                     /locus_tag="PLW15_00390"
     CDS             complement(15746..15961)
                     /locus_tag="PLW15_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32889.1"
                     /translation="MRMAMKHAQGHEPRHSAASVVVPTDFRNPGAVFIQQEIDRDRRS
                     ERWLWLKAVVALVLVAALVVVRQVFFA"
     gene            complement(15958..17454)
                     /locus_tag="PLW15_00395"
     CDS             complement(15958..17454)
                     /locus_tag="PLW15_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT32890.1"
                     /translation="MTIDWATLGYNILTVFLLLLVISGAMPVITTLVLFLAIPFHGSI
                     NNYRKSAPYLPRVAVLVPAWNEEAVIGATIDRFMSLEYPRARLRVYVIDDASTDATAD
                     VVEDRAARYPGAVFHLRRSHGGEGKAHTLNHGLNIVLGEDWAEAVLITDADVIYAPDS
                     LRKLTRHLVDPEVGAVTAYVREGSIRKNYLTRFIAIEYVMAQAAARRAQNVLGAQACL
                     AGGAQLHTRENLVAMGSVIDTSGFAEDTVTTFETQLRGKRVVFDPRAVVLAEEPGTVR
                     ALWKQRLRWARGNVEITARYRAMWFRPNKGHGLGTLGFGLAWFSIQLLPLVMILSSLG
                     LLGLLLVGSDLANVAFRGLWVLAACTYFFSIFLSLQLDWGIGRRTLVEALFFPGIISM
                     IVMLTAFFPGLFEEVLPSAFGIVLTDTAVFWWTVFVYFWISLSMLGGWLAKAVEGTIV
                     GRVLTPVLVYVVGYGPILCAITVDSYIKEFRGAETSWDKTEKTGRVSA"
     gene            complement(17451..18170)
                     /locus_tag="PLW15_00400"
     CDS             complement(17451..18170)
                     /locus_tag="PLW15_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT32891.1"
                     /translation="MKPPRHPELSGDYHVHSTFSDDAVSSLADNIAAAARTGLSVIRL
                     TDHVRVSTTWVPEFVSAVAAQPVPDGLTVLTGVEAKILDASGTLDVPATLAGVDAIVI
                     ADHQFPGSDGPLLPHLVRERLDNGLPVADAIDMLVGALVGAMRRAPGNQLAHCFSILP
                     KIGLSEDDLSDAQLDLWASTAAETSTLIEVNEKWGCPGPRSLGVALAAGAVVVASTDA
                     HEASEVGRYERVPAMLTEARR"
     gene            complement(18167..19153)
                     /locus_tag="PLW15_00405"
     CDS             complement(18167..19153)
                     /locus_tag="PLW15_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT32892.1"
                     /translation="MTRVLVTGAGGPAGVAVIRSLLARDDIDVFAADMDRWASGLYLV
                     AADRRRIVPPAVADGFVDSVRDVVASDGIDVVVSTVDFELRPLALRRAELGAVLAAPS
                     IDTLEVCLDKWMLAQRCEGRLRVPTTALLTAEAIASHWDFPVIVKPRSGAGSRGVRLV
                     EDRAALEAIGPDESQIIQENLPGDEFSVDVLAGADGAVIAAVPRSRTKVDSGVSIAGY
                     TLHSTELEQTAAAVAEAIGLVGVANVQLRYDSAGVAALLEVNPRFPGAMPLTIAAGVD
                     MPSLAVDLALGRPLPVRLPFRELANVRFLEDIFLDPAQIVLAADPQPGESRT"
     gene            complement(19150..20217)
                     /locus_tag="PLW15_00410"
     CDS             complement(19150..20217)
                     /locus_tag="PLW15_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT32893.1"
                     /translation="MSEGAYRVLVVDDDPDVAQYTRTLLERRAECVVMAVGDAHQAQR
                     AMAQFNPDVVVTDIEMPGMTGLELLASLRADRPDLPVVVMTAHVSVDYAVSALRGQAD
                     EFITKPIDSAHLVATVLRLAEEGRARHNPGRPREYVLAVGAHPDDVEIGVGGILASHR
                     ASGDQVVILTLSRGGRGGSPEDRQHESLAAADLLGARLFLEDLVDTEITNTGPTVQII
                     ERVVREVNPTIVYTHSIHDRHQDHRATHEATVVATRSVDTVACFQSPSSTVDFRPTRF
                     VSIDGFTEKKLALLSCFESQSQIRNYLDPEFVLATARYWSRFGAGESVEPLEILRETA
                     DMSMPVRKRPQAQTSSGEGSR"
     gene            complement(20299..21321)
                     /locus_tag="PLW15_00415"
     CDS             complement(20299..21321)
                     /locus_tag="PLW15_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT32894.1"
                     /translation="MSEPFRVIVIEDDPDVALFSKTVLEKRELCVVLTIADPRAVGAA
                     VLDFVPDLVITDIELPGASGLELIGVIRELRPGIPVIVMTAHASLDYAVTALRSQADE
                     FLTKPVTSADLVGHVTRLATKAREAAASTPRKQVVLAIGAHPDDVEVGVGGILAAHAA
                     AGDSVTILTLSRGMRDTGIQGAWTESSEAAGVIGAQVVLEDIPGHALATSPQIAEIIR
                     KVVEELKPTIVYTHSKNDDNQDHRAVHDATLVTTSNVRTVACFQGTAATVEFRPNRFV
                     TIDGFTDKKLAMLECFARDAADRPDYLEPDFALATARSWSRYGQGSYCEPLEIIRDSA
                     DVAGSN"
     gene            complement(21411..22205)
                     /locus_tag="PLW15_00420"
     CDS             complement(21411..22205)
                     /locus_tag="PLW15_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT32895.1"
                     /translation="MAGLGVAQFAPGADTAANLRTIAELAEVAVARGATLVLLPEYSS
                     YFTPEPDEGWLRAAQHLDGEFVTGLGDVARRLGIHLVAGFIETADATHVSNTVVAVTP
                     TGAVAAVYRKMHLYDAFGQRESDWVAPGPIEEPQTFAWDGFTVGLQTCYDVRFPEVSR
                     RLVDAGADLLLVPAEWVRGPLKEHHWRTLLTARALENTVFVAAADHAPPVGVGNSMVL
                     DPMGVELVTIGEETGVAVAHIDPHRIASVRAINPALALRRFGTIPR"
     gene            complement(22205..23026)
                     /gene="nadE"
                     /locus_tag="PLW15_00425"
     CDS             complement(22205..23026)
                     /gene="nadE"
                     /locus_tag="PLW15_00425"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HOT32896.1"
                     /translation="MNPLQARIIYDLNVVPSIDPAEQVAARVGFLKDYLLASKARGFV
                     LGISGGQDSSLAGRLCQLAVEQLRAEGADATFMAVRLPYRVQADEADAQLALDFITPD
                     REITFNIERGVDGVDAEYIEATGDPLLDFTKGNVKARMRMIAQYAIAGQHGLLVVGTD
                     HAAEAVTGFYTKFGDGGADLLPLSGLTKRQGRAMLQHLEAPERLYLKAPTADLLDGLP
                     GQTDEANLGLTYQQIDDYLEGEEVAPEVAVAIEERYLATQHKRREPVTMFDDWWR"
     gene            complement(23127..>23444)
                     /locus_tag="PLW15_00430"
     CDS             complement(23127..>23444)
                     /locus_tag="PLW15_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOT32897.1"
                     /translation="WLRGVGAAAIDNLMEDAATAEISRGQLWQWMHQRVTTAEGTLIT
                     RGLVERELQRTLEQFGGVPGNRFEDAAEVFRSVTLEEDFPTFLTITAYTRYLVDEREA
                     VAA"
     assembly_gap    23445..23544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23545..24901)
                     /gene="aceB"
                     /locus_tag="PLW15_00435"
     CDS             complement(<23545..24901)
                     /gene="aceB"
                     /locus_tag="PLW15_00435"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOT32898.1"
                     /translation="MTTPRIEVLGEYDERFDEILTPEALAFLTRLHDQFAGRRHDRLA
                     ARMHQRKHIENGRDLKFLSETAAIREDATWRVAGAGPGLEDRRVEITGPTDRKMTINA
                     MNSGAKVWLADQEDATSPTWANVIGGQLSLHDAIRRQIDFTSEEGKQYRLGETTPTIV
                     MRPRGWHLVEKHLRYTDRAGLRAPASGSLVDFGLYFFHNAKALIDGGTGPYFYIAKLE
                     NHLEARLWDDVFTFSENALSIPHGTIRATVLIETIPAAFEMEEILYELRDHCAGLNAG
                     RWDYIFSIIKNFRGRGASFVLPDRSSITMTVPFMRAYTELLVSTCHKRGAHAIGGMSA
                     FIPNRRDPEVTARALAAVSADKKREAGDGFDGTWVAHPDLVETARAEFDAVLGDKPNQ
                     LDRLRDDVHVTGADLLDLRIDGVVTDAGVAANVSIAVRYIESWLRGVGAAAIDNLMED
                     AA"
     gene            complement(25006..>25520)
                     /locus_tag="PLW15_00440"
     CDS             complement(25006..>25520)
                     /locus_tag="PLW15_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HOT32899.1"
                     /translation="YRTTPGIETVLARGHAYAPYADLLWVESAEPDLELARTFAESIH
                     REFPGKKLAYNCSPSFNWKSHLDDATIAKFQRELAFMGYAFQFITLAGFHALNHSMYT
                     LARDYSQRHMSAYVELQEAEFASEKDGYTATRHQREVGTGYFDRIATALNPDSETLAL
                     VGSTETAQFH"
BASE COUNT         4270 a         8392 c         8589 g         4069 t
ORIGIN      
        1 tgtcagccat ggctcgagtc taattctgca gagtgaaggc gagtagctcg ggcgcgcgtc
       61 ggtcgacgag acgtccgccc cagaacaccc cgccgatgag cgcgccgacg ccgatcagca
      121 gtccggcccc gagggcggcg aagtgccaga cgggcgagac cgtctcgccg aggaaggcga
      181 ggaacgcgac cgggcccgcc gagagcacgg tcaccgtgaa cgacagcgac tggaccacgg
      241 atcccgtggc gcccgccgcc tgcggctgcg cgaacgggct gtcaccggga tgcacggcgg
      301 gatacgggaa cgcggccgag atgacactcg acactcccag gccgaacagc agaacgctcg
      361 ccgacagccc gatgaagccg ggcagggcgg cccagttgcc ggacaccgta gaggtcacca
      421 cgctgccgat cagcacgagc ggaaccccga tgcccagggc gggcacgagc cggccccagc
      481 ggtcggcgcg accggcgaga ttcgccgaga cgtgggccca gaaggcggtg ctgtcgtgcg
      541 cgacgtcgtt gtgcaccgtc cacccgagga acaggcacat gacgggcacc ggcagccagg
      601 ccagcaggcc gatcggcacg ccggcgatca gcagcgcccc caccatcacg atcggtacca
      661 cagggatcac ggcgagaccc acccggtagc gcgcgtcacg actccagtag ctgaggctac
      721 gggcggcgac cgccccggtc ggcgtggtgg gcacgcgttc gaaccagccc agacccgtgt
      781 acgaccgggc catcccctca cgctgcgggg tggtcagcat gacgccgacg agccagcgcc
      841 acacgagtgc cagagcgatc aggaaggcga ccgcgatcag gatcttcagg ggaacctgac
      901 cccatcgacc gtccgctgca tcggccggga tgctccaggc cgcaccgaga ggcgtccacg
      961 tgagcacggc ggcgatccgc cgcaggatcg ggagtccgcg cgatccccag tcgatcgtcg
     1021 ccatgatcgc cagcagcggc gcgagcagag caagcactgc aaccaggatg accgccgtag
     1081 tctcccgcac ccgtcgggtc gccagaacca gggatgcgac ggcgctcgac acgcgtgcgg
     1141 ccagcacgca cgtcgccacg atcagaaccg cagcaagcac cgcgagcccg gccgccgggc
     1201 cagtcgaact ccaggtcacg atctgcgcga ccgcgaagat cactatgagc agcgacggga
     1261 tgctgaccag agccgagatc gcgagagcta tcgagagccg agtggtctcg ataccgaaga
     1321 ggctgaaccg gcgcgggtcg atggtgtcat cgaccccgaa cgccaacggc agcagaagaa
     1381 acgccagcac cacgatcgat ccgaagacga tcaccactgt gcgcgagata tcggtgctcg
     1441 caaatcgcag tgacacgagg ccgacgacga tgaacgcggc gacacccagc ccgtaggtga
     1501 gcgcgatgag catgcccacg agttgggctg ggcttcgccg gaacgagttg cccagcaggg
     1561 tcagcttcag tcggagaaac tgtgcaacca ctcgagcccc tccaccgtct cccggccgcc
     1621 cgcgagctca acgaaacgct cctcaagggt gactccgttt cgtacgtcgg ccatcgtgcc
     1681 gtcggccagc accgaaccgt cgacgatcac cgcgacgtag tcgcagatgc gctgcaccag
     1741 gtccatgctg tggctcgaca gcagcaccgt tcccccggcg gccacgtact tctgaaggat
     1801 ctcggtcacc gtggccgagg agaccgggtc gacagactcg aacggctcgt cgagcacgag
     1861 cagccgcggc gagtggatca tcgccgccgc gagagcaatc ttcttggtca tgcccgccga
     1921 gtagtcgctc accaaccgag acagtgacgc ctcgaggccg aaggcgtggg ccaagtcggc
     1981 cgtcctggcg cggaccgtct cgccgtccat gccgcgcagc gcacccgcgt agaacagcaa
     2041 ctgggcgccg gtgaggcggt cgaacaacct gaggcggtcg ggcagtacac ccatgttccg
     2101 tttggcgacg acggggtcgc tccagacatc cgagccatgc accgtcacgg ttccggnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgg cgacgacggg gtcgctccag
     2281 acatccgagc catgcaccgt cacggttccg gccgtggggc gcagcagccc cgtgatcatc
     2341 gacagggtcg tggtcttgcc cgctccgttg gggccgacga tgccgtagaa cgcgcccggc
     2401 gcgatctcga ggtgcacgtc gttcacggcg agcttgtcgt cgaagcgctt ggtgagcgag
     2461 tccacgcgca ggatcggcgg aacggtcttg cccttcttgc gagcgacgct cttggtcgcg
     2521 ggcgtcacga ccgccaccga gtcggccgga tgctccggct cagcttcggg ctcggttgca
     2581 gcctcgattt ccggctcggt ttccagctcc gcttccggct caacctctgc ctcgggctca
     2641 agctcaggct ctgcctcggg ctcaccgtcg ggctcgggag tgggatcgga tggctcggcg
     2701 accagcgttg gctcatcggg cgcctcgacc ggagcagcac cctggctctt cgaacgaccg
     2761 agcatgatcg tgggaatcga cagcttggga acggtgaaac ccagaacccc tgacgacgtg
     2821 gcctcgctcg tcgccggctc ggcttcgatg accgtcgggc cgtccgtcgt ctcgtcaggc
     2881 tcggtgacag ccgctggcgg cggcacggct cccgaagagg ctccggctgc cgcaggctgt
     2941 cccgacgggc ggccggtggg cttcgccgtc ggctttcgag cgggagaggg cttgcgcgct
     3001 cctgtccccg gcttggtcgg gattctccgc tggggtttct tcgccgggcc aggctctggg
     3061 acgttttcca ccgtcccaac ctaccaacgg tcgggcgacc cgttgagctt gcatcacgaa
     3121 tgcgcaacat cccgcgcatt ctcgtgaccc tctcaggcac cgccgatact ctggtaccag
     3181 cacaaaggtg tgtccaatca cattcccatc ggtgaacagc aggcgaattc ttcgttctca
     3241 ccagggatca cgacacaacc cttgaggaga cctctatgac cacccagatc gtcattctgg
     3301 ccgccggaat gggcagccgc ctcggccgca gccttccgaa gcccctgacc gagttggctg
     3361 acggccgcac gatcatgcag cagcagttcg acaacatccg gcacgccttc ggcaacgagg
     3421 cgcacgtgac gatcgttgtc ggctacaagc tcgagcacat catcgaggca ttccccgacg
     3481 cgtcattcgt ctacaacgag cagtacgacc agacgaacac ctcgaagagc ctgatgcgtg
     3541 cgctgcaggc atccactccc ggtggcgtgc tgtggatgaa cggtgacgtc gtgttcgacc
     3601 ccaccgcgct cgagcgtgca gccctgatgg tcgcccgcga ccagtcgttc gtctccgtga
     3661 acaccgccaa ggtgtccgac gaggaggtca agtacacgac cgacgccgag ggcttcatca
     3721 aggaactgtc gaagaccgtc actggtgggc tcggcgaggc cgtgggcatc aactacatct
     3781 cccgcaacga caaggcggtg ctcctgcgcc agctcaagcg tgtcggtgac caggactact
     3841 tcgagcgcgg catcgagctg gcgatcgagc aggacggcat tctcatcgag ccggtcgaca
     3901 tcagcgacct gtacgcggtc gaggtcgact tcgccgaaga cctggagcgc gcgaacctct
     3961 tcgtctgatc tcggcggcct cggccaccga ggggtcatac gataggcacg tgaccaacag
     4021 cgcactgggg cgacccgtct ggagcccggc ggcgcgctac cgacgttcgc tgtggttgct
     4081 cacggttcgc gaccttcggg tgcgctactc cacctcggtg ctcgggtact tctggtcgat
     4141 ccttgatccg ctggtgatgg cggccatcta ctggttcgtc ttcacccagg tgttcgatcg
     4201 caccgtgggg gcgcagccct acatcgtctt cctgttgacg gcgctgctgc cctggatgtg
     4261 gttcaacggc gcgacatccg atgccacccg cgcctacttg cgcgaagcca aactcatccg
     4321 ctcgaccaag attccccgca cgatctgggt cgcgcgcctc gtgctctcga agggcatcga
     4381 gttcgtggcg agtatcccgg tcgtcgtgat cttcgcgatc gccaccggcg cgacgctgca
     4441 gtgggaggcg gcgctcctcc ccctcgcact cctgcttcag gccgtgctca cgatgggagt
     4501 cggtctgatc gtggcgcccc tcgtggtgtt cttccgggac ctggagcgag ccaccaagct
     4561 ggcactgcgc tttctcttct acgcgagccc catcatctac ggactcagcg acctgccctc
     4621 cgggctgcac ttctgggcca ccttcaatcc gctcgcgggc atcttcagcc tgtaccgctc
     4681 agcgttcttc cccgagcagc tggactggtt cgcggtcggc atggcggcga tcatcagcgc
     4741 catcatcttc tgtgtgggtg tcctcgtatt cgcgcgcacc gagcgtgccg tgctcaagga
     4801 gatctaggcc gtgaccgaat ccgagaacgt ggtcatccgc gtcgatgatg ccggcatccg
     4861 tttccgtcgc aaccggcgcg cacggcgcag cttcaaggat ctgttcgccg ggcgcaagcg
     4921 ccgcacccgt ccgggcgagt tctgggctct gcgccacgta tcgttcgccg tgcactccgg
     4981 cgaagcgatc ggagtcgtcg gacgcaacgg tcaaggcaag tcgaccttgc tcaagcttgt
     5041 ggccgaggtg atcctgcccg acgaggggcg agtgcaggtc gccgagggcg tggcgccgct
     5101 catcgagatc accggcgggt tcgttgacga tctcagcgtg cgcgacaacg tgtttctcac
     5161 ggcaggtctg cacgggatga cgcgtgccga gatctccgag cgtttcgacg agatcatcga
     5221 cttcgccgag atccgcgact tcctcgacac tccctacaag cacctctcct cgggtatgaa
     5281 ggtgcgcatc gcgttctccg tcatctcgcg gctcgaggag ccgatcatcc tggtcgacga
     5341 agtactggcc gtcggagaca agggctttcg cgagaagtgc taccgccgca tcgaggaact
     5401 gctcgcggga ggccgcaccc tgttcttcgt gtcgcacaac gagaaggacc tgcggcgatt
     5461 ctgcacgcgc ggactgtatc tggatgccgg atccctcgtg ctcgacggcc ccatcggtga
     5521 cgtgctcgag cggtacaacg ccgactacgc cgagtaaagc cccagcctgc ccatcggcgg
     5581 cgtcgagtac ggtggaggga tggcgaagac tcagagcgtg agcgtctcga accccatgat
     5641 ggttcgggga atcgaccggc tcctcaccgt gcagcggccc gtcgtgctgg cacacattcg
     5701 cagcatccgt cgcgggcgac cgaatgcgtc gccggccgag atcatccgca ccctggagcg
     5761 ccgctacctc acggcggtga ccaccggggg tgccgccgtg ggtgcgactg cggtcgtgcc
     5821 ggcgatcggc acgggtacct cgctggcgct gtcggcggtc gagaccgcag gctttctcga
     5881 ggcgagcgcc ctgttcgcgc agtcgatcac cgaggtgcac ggaatcgtgg tcgacgaccc
     5941 cgaccgtgcg cgcgcactcg tgatgacgat ggtgctcggc acggcaggca ccgaactggt
     6001 cggccagctc gccggacagg tcaccggcag cgcgcccgcc cgcaccgcgt tctggggcga
     6061 gacgatcacg aagaacctgc cccgtgccgt catggggcct atcgcggacc gcatcaagcg
     6121 cacgttcgtg aagaaattcg ccgtcgccca gggcaccaac gtcgtcgggc gactcatccc
     6181 gttcggcatc ggcgcggcga tcggcggtgg cggcaaccac ctgctggggc gccagatcgt
     6241 gcgcaccgca cgcgaagggt tcggacctgc gcccgacacc ttccccagct ggctggagcc
     6301 ggtcatccgt gagccgaagg cacctaaggc gcccaagcct gccaaggatg cccgccctca
     6361 gggccagcgc cgcgaactgc gcatcccgct gccgtccctg ccccgacgcg ccgagaagtg
     6421 ggtcgagtcg ccggaggagt agccgtcagt cggacggcag ggcatcctcg tcgtcatcgt
     6481 cgtcgaacgg atcgccctcg ctgcccaccg agtcaccctc gtggatgcgc gcgttgacct
     6541 cccacagcgc catgaggcgt tcgatggcct catggaatcg tgcggtcgcg acgccgggcg
     6601 tcgtgtcgcc gaagtgccgt tcgacccaga acttgaggcg cttctgcgcc tcggcgtcga
     6661 actgcacggc gtcggcgatc gcgacgatat cgccggcggc gtcagccgtg agccactcgc
     6721 aggcgccgag gtatccggtt tcgtccacct cggctgaggc ggacaccggc cgggtgacga
     6781 tgagcggctt gccggtcgcg agacggtcgt agaccatcgc ggagatgtcg gtgatcgcga
     6841 catccgccgc cgccagctgc cagccgaggc tcggcccgtc gtcgaagatg tgctgcgccg
     6901 ccggatctgc gacattggcc cgggctatcg catcgatgat ctcgcggttc gccgcaccgt
     6961 actcgctgtc cacgacgcca ctgcgcgggt gcggtcggta gatcacccga tgccgtccgg
     7021 tggcgagcag cgctcgaacg agcgtgacgc cgtgggaggc gatcgaaccg taggcggccg
     7081 cgtcacggtc accctcccag gtcggtgcgt agagcacaac ggtgcggtca tcggccgtgt
     7141 acggcaactc gcccgcaaaa tggtcggcct gcgggcggcc gatggcaatc gcgcgagtgg
     7201 cgaggtcgaa gtcccagagc ttgtggctga gccggtcgat ggccgcatca cccgcgacga
     7261 agctgaagtc gtaggccttg aactggttgg tggtcatgta catcttgtcc gactcaccgt
     7321 ggttgatgaa cacgtgccac atgcggccgt agcggaacat ctggaagttc tttgcgttct
     7381 gattcacgta gaagacgatg cgcacgtcct gggtgtgcac gaactcctcg agatcggtca
     7441 cgcgccgcag atacagtgcg ggcacgggcg actcgttcca caactccagc acggcactcg
     7501 ggctgcgcga gatgatcgcg accggccagg tctgactgat ctccgcgagc ggcgcatacc
     7561 actggcggat ctggtacagg ttgaccttgg agtcggcgaa gtagacggcg atcttgatgg
     7621 tgcccggtgc cggtcgcttc tggtcgggtt cccgccgtgc cacctgggcc cgtgtgcgcc
     7681 gggaccgcag gatgccgcgc agcacgttgc gagcggtcct gatgtcatgc agaagcggca
     7741 cggccggtcc ctcccccgag ttaaaggtgt ctctccatca aaccagcggt gggcggccgg
     7801 cgcgtgtcat ccgccacacc gttacccatc tgatgccgcg acggccgccc gggttctcgc
     7861 gccagcccgc gacccagcct ccgaaccacg ccttcagggc ctgcggtcgt cgggcccagc
     7921 gcagaagctg gatgcccgtc cacgatccga catagatcgg cacgaggatc cagggcaggt
     7981 tgcgcttggc cagccacacc cggttgcggg cgttgagccg gtagtagtac gagtggcggg
     8041 tcggttcgat cgccggatga ttcgcgacca gatcaccggc gtagagcacg cggtgcccgg
     8101 tgtcccagac ccgccacgcg agctctatgc cctcgtgcgc gtagaagaac gggtcggccc
     8161 atccgccggt cgcatcgaac acggcgcgag gcagcagggt cgcaccctcc cacacggaga
     8221 agacgttgct cgagtgctcc gggtcgccct tgcggatgcg cggaatccac cggcgcggat
     8281 tctcgacccc gctcgggtcg accacgcgcg gctgcagcag accgatgctc gggtcggcct
     8341 caaggagagc gatcgcgtcg acgaggaagg tcggtgacgg gatgctcgca tcatcgtcga
     8401 ggaagaagat gtagtcgccg ctcacctcgg ccgctccacg attgcggccg gccgggatcc
     8461 ccacgttctc gcgcagcgcg agcgagcgca cgccgcgcgg caggccggtc ggcgtccagg
     8521 cgttgccgac cacgatgacg tcgagcgtga cgccggtctg agcaagcacc gacgcgagac
     8581 cccgagccag gtcatccggt cgggtgccct ggctcagcga gacgacgccc accgtgggaa
     8641 tcaggagcgc actctcttcg acgccatgat cgccacgaag tggccgacca gcgccagaag
     8701 ggccagcgga agcaggatca gcagcaccca ccgctcggcg accagcggcg gcaggaagag
     8761 gccgacgacc gacgcagcga agatgatgat cgtgagctcg accgagtggt acaaccggtg
     8821 gaagggcacg aaccgggccg ctcggcgaag gcgcgcgatg acgcgtccgc tcggcacggc
     8881 ctcctccttg cggtcgctga gcttctcgag accggcattg gctcgagcca cgtgaaccat
     8941 gtcgttgagc gccttgttga gaacaatgag gagcgccagc gcgaggcccg cgctcgtcca
     9001 ggcgaagtcg gcggggaact cgaggggcca gcccgccgcc cggatgccga gcgcgatcgg
     9061 gatgagggcc tcggtcgtgt agtggccgac cttgtcgagg aataccccgg cgggtgacct
     9121 cgtgttgcgc cagcgcgcga cttctccgtc gcagcagtcg acgagcatct gaagctgacc
     9181 gagcacgagc gccagcgccg caccgccgat accggggatc agcagcgccg ccgcggtcga
     9241 ccatcccacg agaatcatga ggcccgtcac accgttggcg gagatgctcg tgcgcagcag
     9301 cagccaggtg aggtagggcg agaggtcacg caggtacagg gacgccgtcc agtgctcggc
     9361 attcgccctc agccgcacct cgggcggctg gctgacctcg cgcagttggg cgatggaggt
     9421 ggggcgcgtg gggtgagcgt cgactgacac tgtcaccttc ccgtgtgtgc ggtgatgttc
     9481 cgggtgatct tcaggaatcc gagaacgaac cccgtgcccc acccgaagtg gatgcacggc
     9541 aagactacga ggtaccagag gcccgtggtg aacccttctc gggccgcgat gacggatgcc
     9601 gcgatcacga gcaccgcgta ggcaccgggt accgcgaacc cgagcagcat ccacggcgtg
     9661 gacaacacca tgccgagcac gcccagaatg agaccgccga agataccgat caccgcgagc
     9721 ggcgggatga agtagcgcac cgagttggcc gtgccgaagc ggcgcgcgag ctcgccgcgc
     9781 cagattcccg tcgcaacgaa ctggcggatg agcttgcgca ggctcgaccg cggtcggtac
     9841 accacctcga gctcgggggt gaaccacacg gtgccgccgg tcgcccgcag ccgacggttg
     9901 agctcccagt cctggccgcg cttgatgtcc tcgtcgaaca acccgacctc gaggaggcgt
     9961 tcgcgacgga acactcccag gtaggcggtg tctgccgggc cagccttgcc gcccacgtgg
    10021 tgctgcgtgc cacccagtcc ctcgggcgag ccgtaggcgt gagcgacggc cttctcgaag
    10081 ggggtgaccc cgacggcctt catgatgccg ccgacgttgt ccgccccggt ctcaagcagg
    10141 gtgcgcaccg cgacgcgcgt gtagtcgggg ggaagcaccg aatgcgcatc cacccggatg
    10201 acgacggggt tgaccgacgc gcgaatcgcc acgttgagcc cgcccggggt cgacccgagc
    10261 tcgttgtcga tgcggcggat gcgcgggtcg gccgccgcca tcgccgagac gatgtcgttg
    10321 gtgccatcca cgctcgggcc gagggccagg atgatctcga agtcgccggc gtagtcctgt
    10381 gcggtcaggc tgttgatggc cgcctcgatg tgctgcacct cgttgagcac gggcatcacg
    10441 taggagactc cgggaagggc atccgacttc gctgccatgg tgtccaatac gtctcgctgg
    10501 taggtcggtt gagcctaccg cgaaggccgg ttgtgcaccg cagaacggcg cgcatcgaaa
    10561 tccgcgagga tgacgatgcg cgccgctcgg tgagtcggaa tcagctgagg ctgcccagag
    10621 tgacgtcgac agtggcgggc ttgccgtcac gcacgtagtt cagggtggcc ttggagccgg
    10681 ccgcaagagc ccgcacctgc gccgtcaggt cggtcttgcc cgtgacgggc aggccgttga
    10741 tgccggtgat gatgtcgccg accgcgagtc cggccgccga cgcggcgccg ccgctggtga
    10801 gctcgacgat gctcgcaccc accaccgtgc tgttggtctg tgtcgtgtcc tcggtgacgt
    10861 cagcgacgct cgcgccgagc agtccgtggg tggccttgcc tgtcgcgatc agctcccccg
    10921 cgatacgctg cgcgagattc gcgggaatgg cgaagcccac accgatgctg cccgactgtc
    10981 cgcccgagct gctgcccgcc gacgcgatcg ccacgttgat tccgatgacg cggccctcgg
    11041 agtcgaggag ggcgccgccg gagttgccgg ggttgatcgc ggcatccgtc tggatgaccg
    11101 acagggagat cgtctgagag gaggaggtcg gggtgttgga ctggttctgt ccggggaggt
    11161 cgaagttcca gaagtcgaac gggctctgcg tgtcgggagc ctgatcgttg ttggtcgagg
    11221 tgtcgggaag cgccgatgat gcgatcgtga tcgagcggtt gagcgcgctc acgattccgt
    11281 cggtgacggt gcccgcgaga ccgagcgggg cgccgatggc gatcgcgccg tcgccgacgt
    11341 tgagcttcga ggagtcagcg aactcagcgg gctggaggtt ggaggtgtcg gccagctgga
    11401 tcaccgcgag gtcggagatc gggtcgattc ccacgatctt cgcggagatg agccgcccat
    11461 cgttggtctg cacctgaatg gtgggatcgg cggcggcacc ctcgagggtg acgacatgcg
    11521 tgttcgtgag cacgtggccc tcggtgtcga tgatgacgcc cgagccggtt cccgacgcgt
    11581 tcggcgacga gaccgagatc gtgacgaccg agggggacgc cttcgccgcg accgcggtga
    11641 tctgagtcgc gttggcgggg tcgttgaccg tgatggtggt cggcgacgac gtcgtcaccg
    11701 acggtgcgcc gctgttcgct gaagtcgccc agagggtcac gccggcgccc gacacgccac
    11761 cgaccaaggc acccactgcc agcgcggcga tgacaccgac cggcgcgatg gtgcggcgct
    11821 gcttggcaag cgcggggtgc tgcgcgggat cgatcggggt cgcggttgcg gcaccgtcga
    11881 cgggggcgga ttcgttcttc tcgggcgagg tggtcatgat gggtgctcct tccagcgagg
    11941 aacagcatcc atcgccaacc tgagcattgt atttggacgg gctgggagga ctctgcggca
    12001 tacccgagcg ggccgatcgg gcacccgacg ctaggctcga accactatga gcgtctcggg
    12061 atcctgggag cgcaccgccc gcggtgccat gttgctcggg cccggcggcg agctgagccc
    12121 gaccatcttc gcggagatgt ctgcgcttgc gacatccacc ggggcaatca acctgggcca
    12181 gggttttccc gacgaggacg gccccgcaga ggtgctcgag gcggcccggg ccgccatcag
    12241 cgccggggtc aaccagtacc cgccgggtct cggcatgccc gtgctgcgcg aggccatcgc
    12301 ggcccaccag aagaggttct acggaatcga cgtggatgcc gcgacccagg tactcgtgac
    12361 ggccggcgcc accgaggcca tcgccgccac cctgctcgcg ctcaccgacg ccggtgacga
    12421 ggtggtcacc ttcgaaccgt tctacgacga gtacggtgca ataacggccc tcggccgggc
    12481 gacccaggtg acggtaccgc tgcgagcgcc gcacttcctt cccgacctcg acgagcttcg
    12541 agctgcggtg aacgatcgca cgcgcgtgat tctggtcaac tcgccccaca atcccaccgg
    12601 ggtcgtcttc ccgcgggaga ttctcgaact catcgtcgaa ctcgcgcacc gccatgacgc
    12661 catcatcgtg actgacgagg tgtacgagca cctcaccttc ggtgtcgaac acgtgccggt
    12721 ggccagcctg ccgggggctt tcgagcgcac cgtgacgatc tccagcggcg gcaagacctt
    12781 cagcaccact ggatggaaga tcggttgggc gatcgccccg gccgaactca tccaggcgat
    12841 cgcggccgtg aagcagtacc tgacctacgt caacggcgcg ccgttccagc ccgccatcgc
    12901 ggtgggcctg ggtctgtccg acgagttctt cgctggtgcg gcggccaccc ttcgggccaa
    12961 gcgagacatc ctgagcgcag ggctggcatc cgcggggttc acggtgggca cacccgacgc
    13021 cgggtacttc gtcatcgccg acgcggcgcc cctgggagtg acagatgctg cggagttctg
    13081 tcgcgcgctg ccctcactcg ccggagtcgt cggggttccc gtgaccgcgt tcgtgcggcc
    13141 ggccaaccga gcgcagtatt cgagcctgat ccgattcgcc ttctgcaagc gcgccgaact
    13201 tctcgaggat gccgcggcac gcctggccgg catgaccgtc gtctcagacg ctgggccgaa
    13261 cctccgcggc aacgcgtgac agcggcagcg tcacgctgaa ctcggtgccg acgccctctt
    13321 cactcgatac ccccatcgcg cccccgtgtg cggtgatgat ggccttcgtg atggtgagcc
    13381 ccaggccgac cccgggcacg gcattgcggg tagcggtggt ggctcggaag aaccgggaga
    13441 acagcatgtc gagttcctcg gccgggatgc ccatgcccgt gtcgcgcacc gtgatgacgg
    13501 cattcgaccc agaagtgctg agctcgacga tcaccttgcc ccctcgcggg gtgaacttga
    13561 gagcgttgga gaccaggttg tccacggcct gaccgagccg agtcgagtcg ccgtgcacca
    13621 tgaccggacc gtcggggagc acgttcagta cctcgatgcc ggcgttgtcc gctgcagggc
    13681 gggcggtctc gatcgacgcc gtgatgatcg cctcgagctc gatgtcccga gcatccagcg
    13741 ggaactttcc tgactcgacc tgcgcggtga acagcaggtc tcctacgagc cgaagcagcc
    13801 ggtgggcgtt gcgctcggcc accgagaggt actggagctg ctcggctgac aggggctggt
    13861 cctcgtcgtc acgaagcagt tccagatacc cgaggatgga gctcagcgga gttcgcagct
    13921 cgtgggagat caggcccacg aactcgtcct tgaggcggga gacctcgcgc gcctgggtga
    13981 catcggacgc gacgaacagg tagccgacgg tgtcgccatc ctcgtcgacg cgcttggtca
    14041 ccgagagctg caccggcacg tgagtgccgt cgggacgcac gtaggtccac tccctcgtct
    14101 ccgacttgcc gaggcgagcc gactcgacca gcgccgagaa gcccgggttg agcactgtct
    14161 cgccgggcgg gtagttgagc tcgcgcgccc gatcctccaa ctcctccggg aggtggaagt
    14221 cgaagatgtg catcttgtcc tcggcgtcat cccggtccgg gccgagcaac ttggtagcgc
    14281 cgggattcca cgcgtcaatc agtccggtgg catcggtgcc gatcacgctc tgctcagtga
    14341 cggcggccca cacgccacgg aacatacgat cggcggcccg gtacttcgac tcactcgcct
    14401 gcagctgcgc agcgaactcg acggtgcgtt ggagcatccg ctccttctcg tccacgagcc
    14461 cacgaatcga gcgcagctgc tcccgtccct ggcgggcgag ctcgttcacg atccccgccg
    14521 cgatggtgag taccagcggg gtgtaggtgc ctcgcaccca ctccgacggc gccgacggcg
    14581 cagtgagccc caggaagacg ggaatgagca gcgcgagaaa ggtgccgaca cccgagtaca
    14641 ggatgtaccg acgcccgttg cccgacgcga gccagacgac gggcagcaca atgagtgcgg
    14701 tgaagggtga catcacgccg cccgtgccgc ctcggaacgc cccgatcgcg aagatatcaa
    14761 tcacaggaac cagcaacgcg aaacgatgcc actcgtgcac tcgggagagc accaccgcga
    14821 gcacggtggc gaatccgacg acagcgacgc cggtccaggc caacgccgga tagttcatgg
    14881 acacgtcggg aacaagcgcg atgataagaa ccgcagccac gacggcaccg agcgtggccg
    14941 actgcttgac gatcggcgat ggtcgattca ggatgttgtg ggtccagacc cgcacgcggt
    15001 ccatggcggt catcgcgctt ccttgcccac cgcaagccac gcgacgaggt cgcgaggact
    15061 gaacggcttc atgaagaagt agtcggctcc cgcgttgatg cccacctcga tggcctgctc
    15121 ctcgaccgac gccgacagca gcacgatgcg cacatcaccg atcgacgagt cggcacgaac
    15181 ggcacgacag acgtcgacgc cgctcatctc gggcatcgca atgtcgagaa tcgcgagatc
    15241 ggggtggtgg gtgcggatcg cctcgagcgc ctcggcgccg tccgcggcga ctgctgcaac
    15301 ggtcagaccc gccttcttga cggcaattgt caccaattgc ctgatgtcag cgtcgtcgtc
    15361 ggcgatgacc acggtgtggg tcatgggatc ctcccgaggg ttgtgaggtc ggcaagcacc
    15421 tggcgcactt gcgcaccggt gaagggtttc ggcaggatcg cgtcggctac ggggaagtcc
    15481 gaggcgtcga gcaccgaggc gacgacgatc gcgcactccg gcacgacctc gcgaaagcgg
    15541 gtgatgagtt cccagccgcc catgccgggg agcatgagat cgatcacggc gagatcgggc
    15601 ggcaggtcgg cggcagcggc cagtgcctcc tcacccgtgc cgacgacgtg cacgacgcat
    15661 ccggccttct cgaagtactg gcgcagaaga ccggcctggt ctgcactatc ctccacgacg
    15721 agcacctgat tccgacgtga ccctgtcatg cgaagaacac ctgcctcacg acgacaagtg
    15781 cggcgaccag caccagtgcc accaccgcct tcagccacaa ccaccgttcg gatcgacgat
    15841 ccctgtcgat ctcctgctga atgaagaccg caccggggtt gcggaagtcc gtcggcacca
    15901 cgaccgacgc ggccgagtgg cgcggctcgt ggccttgcgc gtgcttcatc gccattctca
    15961 tgcggagacc cttccggtct tctcggtctt gtcccaggac gtctccgccc cgcggaactc
    16021 cttgatgtag gagtcgaccg tgatcgcgca gaggatcggt ccgtaaccga cgacgtagac
    16081 gagtaccggg gtgagtaccc ggcccacgat cgttccctcg accgccttgg ccaaccatcc
    16141 gccgagcatc gacaacgaga tccagaagta gacgaagacc gtccaccaga agaccgcagt
    16201 gtcggtgagc actattccga acgcactcgg caggacctcc tcgaacagcc cggggaagaa
    16261 ggccgtgagc atgacgatca tcgaaatgat tccggggaag aacagcgcct cgacgagcgt
    16321 gcgccggccg atcccccagt cgagctggag cgagaggaag atcgagaaga agtaggtgca
    16381 ggcggccagc acccacagcc cgcggaatgc gacattggcc aagtcgcttc ccacgagcag
    16441 gaggccgagg agtcccagtg atgacaggat catgaccaga ggcagcagtt ggatgctgaa
    16501 ccaagcaaga ccgaatccca gcgttccgag accgtgacct ttgttggggc ggaaccacat
    16561 cgctcggtag cgagcggtga tctccacatt gccgcgcgcc cacctgagac gctgcttcca
    16621 tagcgctcgc acggtgccgg gttcctctgc cagcaccacg gcgcggggat cgaacaccac
    16681 cctcttgcct cgcagttggg tctcgaatgt cgtgacggtg tcctcggcga acccactggt
    16741 gtcgatcaca cttcccatcg ccacgaggtt ctcccgggtg tgcaactgtg cccctccggc
    16801 gaggcaggcc tgcgcgccga gtacgttctg ggcgcgccgg gcggcggcct gagccatcac
    16861 gtactcgatg gcgatgaagc gagtgaggta gttcttgcgg atgctgccct cacgcacgta
    16921 ggcggtcaca gctccgacct ccgggtcgac aagatgccga gtgagtttgc ggagcgagtc
    16981 gggcgcgtag atcacgtcgg cgtcggtgat gaggacagct tcggcccagt cctcgcccag
    17041 aacgatgttc agtccgtgat tgagcgtgtg tgccttgccc tcacccccgt gcgaccgccg
    17101 aagatggaac acggcacccg ggtagcgggc ggcccgatcc tcgacgacgt cggccgtggc
    17161 atccgtgctg gcatcatcga tgacgtagac acgtagtcgc gcacgcgggt actcgagaga
    17221 catgaaacgg tcgatggttg cgccgatcac ggcctcttcg ttccatgcgg gcacgagaac
    17281 ggcaacccgc ggcagatagg gcgcggactt gcgatagttg ttgatgcttc catgaaaggg
    17341 aatcgccagg aagagcacca gcgtggtgat gaccggcatg gccccggaaa tcacgagcag
    17401 cagcaggaac actgtgagga tgttgtagcc gagggtggcc cagtcgatgg tcatcgtcgg
    17461 gcctccgtca gcatcgccgg cacgcgctcg taccgtccga cctccgacgc ttcgtgcgcg
    17521 tcggttgagg cgacgaccac tgcgccagct gcaagcgcga cccccagcga gcgcgggccg
    17581 gggcagcccc acttctcgtt cacctcgatg agggtggatg tttcggcagc cgtcgaggcc
    17641 cagaggtcga gctgggcatc cgacagatcg tcctcgctga gcccgatctt gggaagaatc
    17701 gagaagcagt gcgctagctg gtttcccggc gctcgcctca tcgcaccgac gagggccccg
    17761 acaagcatgt cgatcgcatc ggcgaccggc agtccgttgt cgagtcgctc tcgaaccaga
    17821 tgcgggagca acggtccgtc ggagccgggg aactgatggt cggcgatgac tatcgcgtcg
    17881 acgcccgcca gagtggccgg cacgtcgagg gtgcccgagg cgtcgaggat cttggcctcg
    17941 actcccgtca gcactgtgag accgtcgggc acgggctgtg ccgcaacggc ggagacgaac
    18001 tcgggcaccc aggtcgtgga gaccctgacg tgatcggtca gccggatgac actgagaccg
    18061 gtcctggcag ccgccgcgat gttgtcggca aggctcgaaa cggcatcgtc ggagaaggtc
    18121 gagtggacgt gatagtcccc cgacagctcc ggatgccgtg gcggcttcac gtgcggctct
    18181 ctcccggctg cgggtcggcc gcgagcacga tctgcgccgg atccaagaag atgtcttcga
    18241 ggaagcgcac gttggcaagc tcccggaacg gcagtcgcac gggcagcggg cgacccaagg
    18301 cgagatccac cgcgagggag ggcatgtcca cgccggccgc gatcgtgaga ggcatcgcac
    18361 ccgggaaccg cgggttgacc tcgagcagcg cggcgactcc ggcgctgtca tagcgcagct
    18421 gcacgttggc gacccccacg agtccgatgg cttcggcaac cgcggcagcc gtctgctcca
    18481 actcggtgga atgaagggtg tagcccgcaa tggacacgcc cgagtcgacc ttcgtgcgac
    18541 tgcggggaac ggcggcgatg accgctccgt cggctcccgc gagaacatcg accgagaact
    18601 cgtcaccagg gaggttctcc tggatgatct gcgactcatc ggggccgatc gcctcgaggg
    18661 cggcgcggtc ctcgacaagc cgcacaccgc gggagcccgc gccgctgcgg ggcttgacga
    18721 tcaccgggaa atcccagtgc gacgcgatgg cctcggcggt gagcagcgcg gtcgtgggca
    18781 cccgcagccg accctcgcag cgttgggcga gcatccactt gtcgaggcag acctcgaggg
    18841 tgtcgatcga gggggctgcg agtacggccc cgagctcagc tcggcgcagc gccagcggtc
    18901 gcagttcgaa gtcgacggtc gacacgacca cgtcgatgcc gtcggaagcc accacgtcgc
    18961 gaaccgagtc gacaaatccg tcggctacgg cgggcggcac gatccgtcgt cgatctgcgg
    19021 cgacgaggta cagaccgctc gcccagcgat ccatgtcggc ggcgaacaca tcaatgtcgt
    19081 cgcgagcaag cagcgagcgg atgacggcca ccccggccgg gccgccggct cccgtgacga
    19141 gtacacgagt catcggctac cctcccccga gctggtctgc gcctgcggac gcttgcgcac
    19201 cggcatggac atgtcggcgg tctcgcggag gatctcgagc ggctcgacgc tctcgccggc
    19261 accgaatcgc gaccagtacc gcgccgtggc gagcacgaac tcggggtcaa gatagttgcg
    19321 gatctgggac tgcgattcga agcacgacag cagcgcgagc ttcttctcgg tgaacccgtc
    19381 gatcgagacg aaccgggtgg gtctgaagtc cactgtcgac gacggactct gaaaacaggc
    19441 gaccgtgtcg accgagcggg tcgcgacgac cgtcgcctcg tgagtggcgc ggtggtcctg
    19501 atggcgatcg tggatcgagt gggtgtagac gatcgtggga ttcacctcgc gcacgacgcg
    19561 ttcgatgatc tgcacggtgg ggccggtgtt ggtgatctcg gtgtcgacca ggtcttcgag
    19621 gaacaggcgc gcgcccagga ggtcggcggc ggcgagcgac tcatgctggc gatcttcggg
    19681 cgaaccaccc cttccgccgc gggagagggt caggatcacg acctggtccc ccgacgcgcg
    19741 gtgcgaggcg aggatgccac cgacaccgat ctcgacgtcg tcgggatgcg cacccacggc
    19801 gagaacgtac tcgcgcgggc ggccggggtt gtgccgcgca cgaccctcct cagccaatcg
    19861 gagcaccgtc gccaccaggt gcgcagaatc gatcggcttc gtgatgaact cgtcggcctg
    19921 gccgcgaagg gcgctgaccg cgtagtcgac cgagacatgc gccgtcatca cgacaaccgg
    19981 cagatcgggc cgatctgcgc gaagcgacgc gagcagttcg aggcccgtca tgcccggcat
    20041 ctcgatgtcg gtgacgacga catcggggtt gaactgggcc atcgcgcgtt gcgcctgatg
    20101 ggcgtctccc acagccatca ccacgcactc ggcacgccgc tccaggaggg tgcgcgtgta
    20161 ctgggccaca tcgggatcgt cgtcgacgac gagcacccga taggcgcctt cagacatcac
    20221 gtaccccctc gtggtaactc tagggccggc ccaccggcga ggcgcggcgg cccaacctca
    20281 ggcgctcggc tgcgcgattc agttcgaccc ggcaacatct gccgagtcgc ggatgatctc
    20341 caacggctca cagtacgagc cctggccgta tcgggaccac gaacgggccg tggccagtgc
    20401 gaagtcgggt tcgaggtagt cggggcgatc tgcggcgtcg cgggcgaaac attcgagcat
    20461 ggccagcttc ttgtcggtga agccgtcgat cgtgacgaac cggttggggc ggaactcgac
    20521 ggttgccgct gtgccctgga agcacgcgac ggtgcgcacg ttggacgtcg tcacgagagt
    20581 cgcatcgtgt acggcgcggt gatcctggtt gtcgtcgttc ttgctgtgcg tgtagacgat
    20641 ggtgggcttc agctcctcga ccaccttgcg gatgatctcg gcgatctgcg gcgacgtcgc
    20701 caaagcgtgg ccgggaatgt cctccagcac gacctgggca ccgatcacgc cagcggcctc
    20761 cgaactctcg gtccacgccc cctggatgcc cgtgtcgcgc atgccgcgcg acagggtgag
    20821 gatcgtgacc gaatcccccg cagcggcgtg agcggccagg atgcccccga ccccgacctc
    20881 gacatcatcg gggtgggcac cgatcgcgag caccacctgc ttgcgcggcg tggatgccgc
    20941 ggcctcccgc gccttcgtcg ccagccgggt gacgtgcccg accagatcgg cggaggtcac
    21001 cggcttggtg aggaactcgt cggcctggct gcgcagggca gtcacggcgt agtcgagtga
    21061 ggcgtgcgcc gtcatgacga tgacggggat gcccgggcgc aactcgcgga tcacaccgat
    21121 gagctcgagg cccgaggcac cgggcagctc gatgtcagtg atcaccagat cgggcacgaa
    21181 gtcgagcaca gctgcgccca ccgctcgcgg atcggcgatc gtcaggacga cacagagttc
    21241 ccgcttctcg agcaccgtct tggagaacag cgcgacatca ggatcgtctt cgatgacgat
    21301 gactctgaat ggttcggaca tgaccagacc ccctctggat gcgatgtacc caactgtacc
    21361 ccgattggcc tgccgcgccc acgaggtgtc acccccaaac cgggtgatgt ttaccggggt
    21421 atcgtgccga atcgccgcag tgcgagggcc ggattgatgg cgcgcaccga cgcgatgcgg
    21481 tgggggtcga tgtgggcaac cgccacgccg gtctcctcgc cgatcgtgac gagctcaacg
    21541 cccatcgggt cgagcaccat gctgttgccg accccgaccg gtggcgcgtg gtcggcggcc
    21601 gccacgaaca ccgtgttctc cagcgcccgt gccgtgagca gtgtgcgcca gtgatgctcc
    21661 ttgagtggtc cgcgtaccca ctcggccggc accagaagca ggtcggcgcc agcgtcgacg
    21721 aggcggcggc tcacctccgg gaagcgcacg tcgtagcagg tctggaggcc cacggtaaag
    21781 ccgtcccagg cgaacgtctg gggctcctcg atggggccgg gggcgaccca gtccgattcg
    21841 cgctgcccga aggcgtcgta caggtgcatc ttgcggtaca cggcggcgac ggcgccggtc
    21901 ggggtgacgg cgacgaccgt gttgctgacg tgcgtggcat ccgcggtctc gatgaagccg
    21961 gcgaccagat ggatgcccag ccgcctggcc acgtcgccca acccggtcac gaactcgccg
    22021 tcgaggtgct gagccgcgcg cagccagccc tcgtcgggct cgggagtgaa gtaggacgag
    22081 tactcgggca gcagcacgag ggttgcgccg cgggccacgg cgacctccgc gagttcggcg
    22141 atggttctca ggttggccgc ggtgtcggcg ccgggcgcga actgcgcgac accgagtccc
    22201 gccatcaccg ccaccagtcg tcgaacatcg tcaccggctc gcgccgcttg tgctgggtgg
    22261 ccaggtagcg ctcctcgatc gcgacggcga cctcgggcgc gacctcctcg ccctcgaggt
    22321 agtcgtcgat ctgctggtag gtgaggccga ggttcgcttc atcggtctgc ccgggcaggc
    22381 cgtcgagcag gtcagccgtg ggtgccttga gatacaggcg ctcgggagcc tcgaggtgtt
    22441 gcagcatggc gcgaccctgc cgcttggtga gcccggagag cgggagcagg tcggccccgc
    22501 cgtcgccgaa cttggtgtag aagccggtca cggcctcggc cgcgtgatcg gtgccgacga
    22561 cgagcagccc gtgctggccc gcgatcgcgt actgggcgat catgcgcatg cgcgccttga
    22621 cgttgccctt cgtgaagtcg agcagcggat cgcccgtggc ctcgatgtac tcggcgtcca
    22681 ccccatcgac gccgcgctcg atgttgaacg tgatctcgcg atcgggggtg atgaagtcga
    22741 gggcgagctg ggcatccgcc tcgtcggcct gcacccggta gggcaggcgc acggccatga
    22801 acgtcgcgtc ggcaccctcg gctcgcagct gctcgaccgc aagctggcag agccgcccgg
    22861 cgagggacga gtcctggcct ccgctgatgc cgagcacgaa gcctcgggcc ttcgatgcca
    22921 gcagatagtc cttgaggaag cccactcgcg cggcgacctg ttcggccggg tcgatcgagg
    22981 gcaccacgtt gaggtcgtag atgatgcggg cctgcagggg attcatgact cgaatctacc
    23041 gccggattcc ggctggttga gtagcgcgaa agcgcgtatc gaaaccatct agggtctcga
    23101 tacgcgcttc gcgctactcg accgacctag gcggcgaccg cctcccgctc gtccacgagg
    23161 tagcgcgtgt aggccgtgat cgtgaggaag gtcgggaagt cctcctcgag ggtcactgag
    23221 cggaacacct cggcggcatc ctcgaaccgg ttgccgggaa ctcccccgaa ctgctcgagc
    23281 gtgcgctgaa gttccctctc cacgagcccg cgtgtgatga gggtgccctc agccgtcgtg
    23341 acgcgctgat gcatccactg ccagagctgg ccccggctga tctccgcggt cgcggcgtcc
    23401 tccatcaggt tgtcgatggc ggcagctccg actccgcgca gccannnnnn nnnnnnnnnn
    23461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnntcgcgg cgtcctccat caggttgtcg atggcggcag
    23581 ctccgactcc gcgcagccag ctctcgatgt agcgcacagc gatggacacg ttcgcggcga
    23641 ccccggcatc cgtcaccaca ccgtcgatgc gcaggtcgag caggtcagca ccggtcacgt
    23701 gcacgtcgtc acgcagccga tcgagctggt tcggcttgtc gcccagcacc gcgtcgaact
    23761 cggcgcgcgc cgtctccacg aggtcaggat gcgccaccca ggtgccgtcg aaaccgtcgc
    23821 cggcctcgcg cttcttgtcc gcactgaccg ccgccagcgc tcgcgcggtc acctcggggt
    23881 cgcggcgatt ggggatgaac gcgctcatgc ctccgatcgc atgcgccccc cgcttgtggc
    23941 aggtggagac gagcagctcg gtgtacgccc gcatgaacgg cacggtcatc gtgatgctcg
    24001 accggtcggg cagcacgaag ctcgcgccgc gcccgcggaa gttcttgatg atcgagaaga
    24061 tgtagtccca gcggccggcg ttcaggcccg cgcagtggtc gcgcagctcg tacaggatct
    24121 cctccatctc gaacgccgcg gggatcgtct cgatcagcac cgtcgcgcgg atggtgccgt
    24181 gcgggatgct cagggcgttc tccgagaacg tgaacacgtc atcccacagc cgcgcctcga
    24241 ggtggttctc gagcttcgcg atgtagaagt acggcccggt gccgccgtcg atcagcgcct
    24301 tcgcgttgtg gaagaagtac agcccgaagt cgacgagcga ccccgacgcg ggggcgcgca
    24361 gcccggcgcg gtcggtgtag cgcaggtgct tctcgacgag gtgccagccg cgcggtcgca
    24421 tcacgatcgt cggagtggtc tcccccagcc ggtactgctt gccctcctcg ctggtgaagt
    24481 cgatctggcg acgaatcgcg tcgtgaaggc tcagctgccc gccgatcacg ttggcccagg
    24541 tcgggctcgt ggcatcctcc tggtcggcga gccacacctt ggcgcccgag ttcatggcgt
    24601 tgatggtcat cttgcggtcg gtcggaccgg tgatctcgac gcggcggtcc tcgaggccgg
    24661 gcccggctcc ggcgacgcgc cacgtggcat cctcgcggat ggcggcggtc tcggacagga
    24721 acttgaggtc ccggccgttc tcgatgtgct tgcgctggtg catccgtgcc gcgaggcgat
    24781 cgtggcgacg accggcgaac tggtcgtgca ggcgggtgag gaaggccagg gcctccgggg
    24841 tcagaatctc gtcgaagcgc tcgtcgtact cgccgagcac ctcgatgcgg ggtgtggtca
    24901 tggtcgtgcc tttcggttgg tggctggttg agtagcggcg aagccgcgta tcgaaaccgg
    24961 gagcggggtc tcgatacggc ctgcggccta ctcgaccggc ggggatcagt ggaactgtgc
    25021 ggtctcggtg ctgcccacga gtgcgagggt ctcgctgtcg gggttgagcg ccgtggcaat
    25081 gcggtcgaag tagcccgtgc cgacttcgcg ctggtggcgc gtcgccgtgt agccgtcctt
    25141 ctcggacgcg aactctgcct cctgcagctc gacgtaggcg ctcatgtggc gctgcgagta
    25201 gtcgcgcgcg agcgtgtaca tcgagtggtt gagggcatgg aaccccgcga gggtgatgaa
    25261 ctggaacgcg tagcccatga atgcgagctc ccgctggaac ttcgcgatgg tcgcgtcatc
    25321 caggtggctc ttccagttga agctggggct gcagttgtag gcgagcttct tgccggggaa
    25381 ctcccggtgg atcgactcgg cgaacgtgcg agcgagctcc aggtcgggtt cggccgactc
    25441 cacccacagc aggtcggcgt acggcgcgta ggcgtggccg cgggcgagca ccgtctcaat
    25501 gccgggggtc gtgcggtaga
//
LOCUS       DAOSOU010000004        24796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2021_length_24796_cov_6.851097, whole genome shotgun sequence.
ACCESSION   DAOSOU010000004 DAOSOU010000000
VERSION     DAOSOU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 24796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24796
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_24796_cov_6.851097"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLW15_00445"
     CDS             <1..251
                     /locus_tag="PLW15_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT32900.1"
                     /translation="LPPFLDAQSIDGKKVLLVDDVSDSGRTLDMVVTLLEGMGAEVRT
                     VVLYTKPGTVHEPAYTWRRTAQWITFPWSAEPPVTLEA"
     gene            265..942
                     /locus_tag="PLW15_00450"
     CDS             265..942
                     /locus_tag="PLW15_00450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT32901.1"
                     /translation="MAPKPLAELVDPGWAAALGPVESQIAAMGEFLRAETAAGRRYLP
                     AGDRVLRAFTYPLADVRVLIVGQDPYPTPGHPIGLSFAVERDVRPVPRSLANIYKELH
                     DDLGIAPAPHGDLTPWAEHGVMLLNRVLTVQAGASGSHRGKGWEAVTEAAIRALVARG
                     GPLVAILWGRDAANLKPLLGSTPTITSVHPSPLSAHNGFFGSRPFSRANELLVAQGAE
                     PVDWRLG"
     gene            1003..1944
                     /locus_tag="PLW15_00455"
     CDS             1003..1944
                     /locus_tag="PLW15_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT32902.1"
                     /translation="MRTRTKEDGTTLQFTNLSKSFGAVRAVSDLSFTVEPGRVTGFLG
                     PNGAGKTTTLRMLLGLVNPTSGVATIGGRRYSELEKPLATVGTALEASDFHPGRSARN
                     HLRVYAAAAAIATARVDEVLGIVGLTEYADRRVGGYSLGMRQRLGLAFALLGDPDVLV
                     LDEPTNGLDPEGIRWMRGFLQSLAAEGRTVLVSSHLLAEVQQTVDWVVIISRGRSVYE
                     GSLDGLETGQVVRIDSSDRAALTNALSSAGAEVRATDDALLATGITAGEAGAAALAAG
                     VPLSLLITESVGLEEVFLQLVGSDADSIADGIAQVSA"
     gene            1941..2759
                     /locus_tag="PLW15_00460"
     CDS             1941..2759
                     /locus_tag="PLW15_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32903.1"
                     /translation="MIGALRSEFLKLTTVRLWWVLLLTLFGYVGFTSGLLAGVFGALG
                     DQLATSPNAPQLPADALPPLIYSTVTAVGYVIPLILGALSVTSEFRYQTLTPTFLAGP
                     RRGVVLGAKVMVLAAAGAVFGIVGLVSSMGIGGSILAFSGIDPQLGSADTWLLAGRVV
                     IASALWAVIGVGVGSLITNQVASIVVVLAFAQFVEPILRLGASIWDWTAAVGKFLPGA
                     ASDALVGSSIFTSLGTGTTSVQTLEWWQGGLVLLGLGVVLALAGFLTSWRKDVT"
     gene            2790..3368
                     /locus_tag="PLW15_00465"
     CDS             2790..3368
                     /locus_tag="PLW15_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOT32904.1"
                     /translation="MLEEEYQPRRKLPPHLAPKPAPETPFAYTIRDATVTDLPHVREI
                     YNHYVLNSTVTFDEKPMTLADFKRKFAKVQEKKMPFLVAENPAGQLLGYAYVYPWKEK
                     AAYRYTVENSIYLGPASTGKGLGKALLAELIDRSRDAGLKEIIAVIADKGAEASIAMH
                     ENFGFKEIGHMGKVGFKFDRWLGTVLMQKSLK"
     gene            complement(3361..3906)
                     /locus_tag="PLW15_00470"
     CDS             complement(3361..3906)
                     /locus_tag="PLW15_00470"
                     /inference="COORDINATES: protein motif:HMM:NF037009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT32905.1"
                     /translation="MSLTPKGEARRAALLDAVLRVLEREGPGAVTHRSVAREAGVPLA
                     AATYYFDDLDDLYVSALRRATEEQVALFAGTEMNDLRAVARLIHRWATENRGAALAQY
                     ELMFLAMRRDALRADAQAWYGALEHAIDPHGKHPRQTRVRALALDGLLLRILWLGEPS
                     TVDETEKALREIVEGTRASAT"
     gene            complement(3903..4235)
                     /locus_tag="PLW15_00475"
     CDS             complement(3903..4235)
                     /locus_tag="PLW15_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HOT32906.1"
                     /translation="MGYVFLGLAIVGEVIATTFLKFTSGEKAVWWAYAIVGVGYVFAF
                     GMLSLALGRGVPLGTAYAIWAGVGVVLVAVISWLAFGESLTWQQVLGIVLVTGGVVLL
                     EAGGKHSA"
     gene            4303..4728
                     /locus_tag="PLW15_00480"
     CDS             4303..4728
                     /locus_tag="PLW15_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT32907.1"
                     /translation="MITPPTQLRLVVETEDFDAALAFYRDALGLPELEAYEGEGGARV
                     VILSVPAATLELSNPAQVRLIDEVEVGHPVARPYPLSIRVAFEVADAAATTTALEAGG
                     ATVVAPPTLTPWNSLNSRLDAPGGLQITAFQELGQVPPS"
     gene            complement(4729..>5291)
                     /locus_tag="PLW15_00485"
     CDS             complement(4729..>5291)
                     /locus_tag="PLW15_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32908.1"
                     /translation="QWSGASLYIFVYMMVVAIQAVNVTFPFALGYGVTRRDFFLGTAV
                     QFVVLATMYATGLTILAAIEKATNGWGLGGRMFTAAYFGSDLPWYLQFFVFFVLLLFF
                     FFVGAVLGAVYVRWKATGVTFFLVALGALVIVALYLLTLTDSWPAVGEFFAAAGFVGT
                     YAWSLVITAISGVAGYLILRRATPRA"
     assembly_gap    5292..5391
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5392..5834)
                     /locus_tag="PLW15_00490"
     CDS             complement(<5392..5834)
                     /locus_tag="PLW15_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT32909.1"
                     /translation="MTAVIEVDGLTRRYGRLAAVDDATFTLEGNRIHGLLGRNGAGKT
                     TLMQLLTGQEFASSGTIRVFGQSPVENAGVLQHVSFIKESQRYPEDFRPKHVLRSAPW
                     FFENWDAEFAEQLVDDFRLPLNTRIKKLSRGQLSAIGVIVGLASRA"
     gene            complement(5831..6184)
                     /locus_tag="PLW15_00495"
     CDS             complement(5831..6184)
                     /locus_tag="PLW15_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT32910.1"
                     /translation="MDESRPIFLQVAEQIENDIIMGALAEEDQVPSTNEFAAFHRINP
                     ATALKGVTLLVDAGILYKKRGIGMFVATGARQRILDQRRAVFEAEFVRPLITEAAKLG
                     ITSAQLIGMIKKEEK"
     gene            6270..7679
                     /locus_tag="PLW15_00500"
     CDS             6270..7679
                     /locus_tag="PLW15_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT32911.1"
                     /translation="MFELHHLTALEQLDWLRRGQVSPLELAEHYLERIARLDPDIGAF
                     ATVTADAARARAATLESLVPRAAGLWGLPSADKDLWARAGVRTTFGSRHFHDFVPDES
                     DEIVQTLDGAGAVSLGKTATPEFGMPAYTESALLGATRNPWNPELGVGGSSGGAAAAV
                     AAGLLPLAPGSDGGGSIRIPAAATGLVGLKPSRGRVASGSGFTTLGGLVTYGPLARTV
                     SDAALLLDALIGDSEWATQPPTWDGGAYLNAAIRGEGRYQLGVMTSSPWDDSHEITVS
                     PEARATLDLAVAELDALGHGIDTVSLPPSPDYPDAFRTLWQAGASAIPVEGQELERLE
                     PLTRWLVERGRALDARRLTEALEYLAGFERGVIRQFAGFDAVLTPALAHTPPPIGWFD
                     QEDGERNFAQQVQFTPWTSFVNVSGLPAITLPVGQSPDGLPVGVQLVGRPGGEHVLLA
                     LGAQLERRFRWQHRHPSMW"
     gene            complement(7684..8295)
                     /locus_tag="PLW15_00505"
     CDS             complement(7684..8295)
                     /locus_tag="PLW15_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32912.1"
                     /translation="MRDFHARRTALTLIGAGIAVALLAACTPTGGTDTAAATGSPSPS
                     VSASAKPKPTPTASPTPTAAAPFVIDCTSIIPASRQDWLATSGWPLFAPADFFAKVRS
                     EVSSSPYILMQDNGGIVCPYATSSEVVVAYGYAPLADSQVAEADSLIIGTNDGYVTSS
                     YAGGTLYDSATEGNAFEFFLIVPGAMYVATTTEILDEMLATVP"
     gene            complement(8323..9147)
                     /locus_tag="PLW15_00510"
     CDS             complement(8323..9147)
                     /locus_tag="PLW15_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT32913.1"
                     /translation="MSKVAVITGASSGIGANAAARLAAAGWEVAVVGRNPERTRSVAE
                     SVGGTAFLADYDRLDDVRALAASLASRYERIDLLANNAGGLVPSRGLSADGYERTIQH
                     NHLAPYLLTRLLLPVLEASDARVVGTASVANTFSPIRLTDLQWQGRPYLGGWRPYGVS
                     KIAVILFTQELARRSSVEAYAFHPGYVATNFGTESAFMRFGNRVSGGRLAISTEQGAT
                     PLVHLAIAESVGVPSGTYFDGLKPNGRTHRTARDAAAAAELWDRSAALVALPDPVR"
     gene            complement(9144..9584)
                     /locus_tag="PLW15_00515"
     CDS             complement(9144..9584)
                     /locus_tag="PLW15_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32914.1"
                     /translation="MSQEPPPPVTPHPRARIGIVPRLSRVRWLRWLLLTPVVARPGYW
                     FATACALFWGALLGRFHVSRQGGLIVAANLPNWGFGRGGTTVGAVYLTNTNITPGILE
                     HEAVHRAQWKRYGLAFIPLYVSAGQNALHNRFEIEAGLKRGGYV"
     gene            9608..>10044
                     /locus_tag="PLW15_00520"
     CDS             9608..>10044
                     /locus_tag="PLW15_00520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT32915.1"
                     /translation="MAGVIQRVAMVSMHTSPSAAPGVGDSGGMNVAILALAAELAIRG
                     TEVDLLTRAVGAPSSRVLLPGVTLHELPAGSGTLVKQDLASVTDDFGEAVAHLARSTR
                     YDVIHSHYWLSGIAALPVALELGIPLVQSFHTLAVMKDADLGGS"
     assembly_gap    10045..10144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10145..10681
                     /locus_tag="PLW15_00525"
     CDS             <10145..10681
                     /locus_tag="PLW15_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT32916.1"
                     /translation="GRVQPLKGHELAVRALAEIRALRGWAPQLVIVGDATPGHEGFVE
                     ELGALAGQLGVADDIRFVGSLPREDLADLLSVVDLTIVPSFSETFGLVALESAAAGTP
                     VIGYRSGGLRESISEGVSGLLLGTRDPRYWATEIANLLENDERRLELGTSARAFAQRF
                     TWGAAATGLLGVYASVAR"
     gene            complement(10691..12121)
                     /locus_tag="PLW15_00530"
     CDS             complement(10691..12121)
                     /locus_tag="PLW15_00530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT32917.1"
                     /translation="MTTEPQTYDVVILGAGAVAENVADRAVQGGLTALLIESELVGGE
                     CSYWACMPSKALIRSGMALRAAQRLQGAKQAVTGDLDVAAVFARRTVFTHDWDDASQV
                     TWVDGAGISLLRGHARLTGEKRVSVTAADGSVTDVIATHAVVISTGSDPLLPDIPGLV
                     EANPWTPRDATSATSAPDSLAIIGGGVVAVEMATAYASFGTAVTIFARSGLLQGQEDF
                     AAELVTAGLAGLGIDIRRASPTLVERTESGVTVHAGDDSVTASEVLVATGRTPRTTDI
                     GLETIGLEPGEWITVDETLRVPGYDWLYATGDVNHRALLTHQGKYQARAAGDVIAARA
                     KGGPVDDAPWGTHVATADHGAVPQVTFSDPEVASVGITSQAARAAGRNIRVVDYNLGW
                     VAGASLQADDYEGQARMVVDEDRGVIIGVTFVGQDVSELIHAATIAVAGEVPLARLWH
                     AVPAYPTMNEIWLRLLETYGRPVHTA"
     gene            complement(12123..12923)
                     /locus_tag="PLW15_00535"
     CDS             complement(12123..12923)
                     /locus_tag="PLW15_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOT32918.1"
                     /translation="MTDAVETPSRITLEREGAVLLIGLNRPDKRNAADFAMLQQLAIA
                     YAELENDPDLRAGFVFAHGDHFTGGLDLADVGPRLGPDGLDVVPNGGLHPWQVNGQLL
                     SKPVVMAVQGTCLTLGIELMLASDIVVAADSASFAQIEVARGILPFGGATIRFPRAVG
                     WGNAMRWILTGDTFDAAEALRIGLVQEVVPHGEQYARGLELAQRIAAQAPLAVQAALM
                     NAKIAVRDGDAAAEATLQPALGLLAATEDARIGMEAFVTRKPATFVGR"
     gene            13020..13340
                     /locus_tag="PLW15_00540"
     CDS             13020..13340
                     /locus_tag="PLW15_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HOT32919.1"
                     /translation="MNRLAPALAAAAVVAALLSGCTATATYTVSPEDLATTAENALEQ
                     QVGSRPDIDCGTEDIDLVKGTVVPCILTDPASGDRFDAPVTIEEVDGSNYTIGVQVAD
                     APKP"
     gene            complement(13347..14171)
                     /locus_tag="PLW15_00545"
     CDS             complement(13347..14171)
                     /locus_tag="PLW15_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOT32920.1"
                     /translation="MVATFVVIPQWQGSGSSRAMRLVDGAEAIRGDLPSSSTRTIEIP
                     LEAGDGQGTAVHRLSSIALVRDRLAADLATIDGPVITIGGDCGVELAAIEHVAGDDVA
                     VIWLDAHPDLNTPESSPSGAFTGMVLRTLLGEGPAQLVPGTPLKPSRVILAGARSFDP
                     GEDEYIAAHRVVTIGADEVSGQSLVDAVVASGAASVYVHIDLDVLDPEDIQGLGYPEP
                     FGVRSADLVNALKALVARFPLAGAGITEFAPASPRDAVEDMPTILRLVGALASAAG"
     gene            complement(14181..14819)
                     /locus_tag="PLW15_00550"
                     /pseudo
     CDS             complement(14181..14819)
                     /locus_tag="PLW15_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
     gene            complement(14868..15602)
                     /locus_tag="PLW15_00555"
     CDS             complement(14868..15602)
                     /locus_tag="PLW15_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOT32921.1"
                     /translation="MAKQSILGRISQLLKANINALIDQAEDPQLLLDQLVRDYTNSIA
                     EAESAVAQTIGNLRLMEQDHAEDIDAAADWGRKALAASSKADELRAGGNSADADKFDN
                     LAKIALGKQVSSENEAKAVEPTIASQTTVVEQLKSGLDGMRAKLSELTAKRDELVARA
                     KSAAAQSQVIDAVKSIDLLDPTSEISRFEDKIRREEAKVLGQQEIAASTLDAQFESLD
                     DLDKQAEVDARLAALKAGSAPKAVTS"
     gene            complement(15646..17700)
                     /locus_tag="PLW15_00560"
     CDS             complement(15646..17700)
                     /locus_tag="PLW15_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT32922.1"
                     /translation="MWSRIGVGTAILAGLLALGTPAALAEDPVDLSGAYIVDKVRAIT
                     PDEGTEILGALDSLYTRASIQLFVVYVDSFSNPSDPVDWADTVAIDNGLGGNDVLLAV
                     AVDDRQYALSIDDSFTLSDTQITVVEQAIEAELRDDNWAQAAIAGAQSLEAEATGVVG
                     PNQPTDPATPTDPPAESGGGIPILPIVGGVAVVGAGVFIFSRIRRRGSDGRVVSAPEG
                     MTQKQLDQRAGSLLVQLDDSLKTSEQELGFAVAQFGDDATKDFTAVLASAKAKVAEAF
                     TLKQKLDDATPDSPADQRAWTTKIIELCEAADAELDSQADAFDALRELEKNVPQALAA
                     ARDSIGAARARSAEAADTVKALSKMYAASAVAAVSTNIEQAAKLLDFAEAAAGKADTA
                     IAASDSSQAAIAVRTVQASVGQAQQLFTAIDTLKSTLGDATTKLEAAIADTTQDVAAA
                     RALPQDEASAALAPAIAAAAAALSGATATKGDPVASLAKLELANSALDQVFTGVRDEQ
                     QKISSARTQLDAAISAARAQLSSAREYITTRRGGIGESARTRVSEAERHLGQAEALSD
                     SDPVKALGEAQRANDLASQAFDLARSDVSEFTRQDSYGGMPRGSDGADLGGLLGGVIG
                     GMLGSGGGGRSSGGGGWFGGGGSSGSSYRPSRSSRSGSFGGSSRSARSSSGGGRSRGG
                     RF"
     gene            complement(17861..18622)
                     /locus_tag="PLW15_00565"
     CDS             complement(17861..18622)
                     /locus_tag="PLW15_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32923.1"
                     /translation="MPSESELRDLLSSAHAPHELDAQRIIARSRRRRLPRQLAAGAVS
                     VLAVAGISVVVIQTSLVSQPPSTVSMMDQSAEGGAAPEASADSIKRAPADRINLCEAP
                     LADVAPSTLGLQLDVAFPATAPVGTDPVVGAVRLTNTSDLTVSGTTTTVPAITLSRDG
                     VVLWHSNGQAETPERAVTLAPGESIEYEATFVPVQCTVEDDGSEQFRADLPPVPAGDY
                     DLSAALDFLPDAAPTDGSTPGLDLVTGPRSTIQLQ"
     gene            complement(18612..19163)
                     /locus_tag="PLW15_00570"
     CDS             complement(18612..19163)
                     /locus_tag="PLW15_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HOT32924.1"
                     /translation="MSSTAGEPTRWEDVVTRLVAERGDALTRYAFLISGNRDDASDLV
                     QDALVKTFGRVRNGFSIASAEAYVRRAILNTYLDSGRRTSRWRKIAHLNAAPELDEPR
                     DAQTESRLDVTRELMKLTPRERACVVLRYYEDLKVDDIAEWLGISAGAVKRYLSDALA
                     KLAVTLTEDGPAIRDGKGAPRAQ"
     gene            complement(19303..21129)
                     /locus_tag="PLW15_00575"
     CDS             complement(19303..21129)
                     /locus_tag="PLW15_00575"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOT32925.1"
                     /translation="MSIAEITRTQSPVPDGAPESVVAWVDEIAALTLPDDVVWCDGSR
                     AEWDALAREMVASGTLIRLNAEHRPYSFLARSDPNDVARVEDRTFICSVDPVDAGPTN
                     NWMDPIEMRATLQPLFSGSMRGRTMYVVPFSMGPLGSPLSRLGVEVTDSPYVAASMGI
                     MTRIGTEVLSQFTPTTQWVRAVHSVGAPLSPGQDDVPWPCNETKYITHFPESREIWSF
                     GSGYGGNALLGKKSFALRIASVMARDEGWLAEHMLIIKVTNPRGRVFHVAAAFPSACG
                     KTNLAMLKPTIPGWKVETIGDDIAWLGRGEDGRLRAINPEAGFFGVAPGTGYSTNATA
                     MDTIWGNTIFTNVALRDDGDVWWEGMSDTVPAQLTDWEGNPWTPASGRPAAHPNSRFT
                     VAARQCPSISDDWEDPRGVVIDAIIFGGRRATNVPLVVEARDWEHGVYLGATISSERT
                     AAAEGTLGELRRDPFAMAPFCGYNMADHWAHWLSVGAELRKTGSVPRIFQVNWFRKGA
                     DGRFLWPGFGENSRVIEWILERVDGQVAAEESPIGLLPHAGELNVDGLDISDDDLSEL
                     FAIDRETWLAEADATSEFFESFDGRVPAAVTKQLDILRSKLA"
     gene            21300..22678
                     /locus_tag="PLW15_00580"
                     /pseudo
     CDS             21300..22678
                     /locus_tag="PLW15_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(22665..23768)
                     /locus_tag="PLW15_00585"
     CDS             complement(22665..23768)
                     /locus_tag="PLW15_00585"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT32926.1"
                     /translation="MMGSSERSIVRVVIAPDSFKGSLAAEDVADAIAAGWLSERPGDV
                     LTLLPQADGGEGTLDAIEASVPGAVRRSAGAVTGPDGRPTPGEWLELPDGTAVVELAG
                     ASGLPLMTVRDAPAASTRGTGEVIRLALQAGAHSLVLALGGSATTDGGSGALSALGLG
                     LTDDRDRPIAEGGAGLATLARVDRGLLVPAPPGGVTLLTDVTNPLLGPTGAAHVFGPQ
                     KGASPTQCAELDAALARWAQVLGGDPDAAGAGAAGGTAYGFAAVWGARIVPGAPRIAE
                     LTGLTAAAASADLIITGEGRFDDQSLGGKVVGHALGLGRPVAVIAGSLAAAPPGWSAS
                     LTDLAGSVEAATANPRRWLREAGTLAAREFSRS"
     gene            23880..24443
                     /locus_tag="PLW15_00590"
     CDS             23880..24443
                     /locus_tag="PLW15_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOT32927.1"
                     /translation="MSTTTENQLVGLFRQSRTVWAIRAAAALIIGILVLVWPQATVSI
                     LAILLGIYFVILGVVRVIQGFVDKDLNGGGKAANFIIGALILAAGVIVIKNPFETAVF
                     IVLLVGISWIFEGIATLVDTARGHGDGVSILIGILIALAGVVVVLFAPAATTAYAVFF
                     GIVLIIVAILDFVLLFAVGRAVKKAKA"
     gene            24446..>24796
                     /locus_tag="PLW15_00595"
     CDS             24446..>24796
                     /locus_tag="PLW15_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32928.1"
                     /translation="MAESKNSTGAVVKLVVSIAAIVVGLFLVFGATTPAIADGVFVGS
                     ISLVLAIYLWSGVTTGQARTLAIVLILLAAYAFIRGFGLLDLAVLRQLGGIAAIVMGV
                     ILIIPFLRERFGSKS"
BASE COUNT         3696 a         8479 c         8425 g         3996 t
ORIGIN      
        1 tgcttccgcc cttcctcgac gcccagtcga tcgacggcaa gaaggtgctg ctggtcgacg
       61 acgtctccga ctccggtcga actctcgaca tggtcgtgac ccttctggag ggaatgggcg
      121 ccgaggtgcg cacagtcgtg ctgtacacca agcccggcac cgtgcacgag ccggcgtaca
      181 cctggcgccg cacggcccag tggatcacct tcccgtggtc ggccgagccg ccggtgacgc
      241 tcgaggcctg agcgcccaga gaccatggca ccgaagccgc tggccgaact cgtcgacccc
      301 gggtgggcgg cggccctggg gccagtggag tcccagattg ccgcgatggg ggagttcctg
      361 cgagccgaga ctgcggccgg gcgccgctac cttccggccg gggatcgggt gctgcgcgcg
      421 ttcacctacc cgctggccga cgtgcgggtg ctcatcgtcg ggcaggaccc gtaccccacg
      481 cccgggcatc cgatcggcct ctcgttcgcg gtcgagcgcg atgtgcgacc ggttccgcgc
      541 agcctcgcga acatctacaa ggaactgcac gacgacctcg gcatcgcgcc cgcaccccac
      601 ggcgatctca caccgtgggc cgagcacggg gtgatgctgc tcaaccgggt gctgacggtg
      661 caggccgggg catccgggtc gcaccgcggc aagggctggg aggcggtgac cgaagcggcg
      721 atcagggcgc tcgtcgcccg cggtggtccg ctggtggcga ttctgtgggg ccgggatgcc
      781 gcgaacctga agcctctgct cggttcgact cccacgatca cttccgtgca ccccagcccg
      841 ctctcggcgc acaacggatt cttcggctcg cgtccgttca gtcgggccaa cgagctgctg
      901 gtcgcgcagg gcgccgagcc ggtcgactgg cgactgggct gagtctggcc tccgggtcgt
      961 tcggcactgg cgccggtggc gcagatctgt cactattgtt ctatgcgaac tagaactaag
     1021 gaagacggca cgactctcca gttcacgaac ctgtcgaagt cgttcggtgc cgttcgggcg
     1081 gtctccgacc tgagcttcac ggtggagccc ggccgagtga ctggcttcct cggtcccaac
     1141 ggcgcgggca agaccacgac cctgcgcatg ctgctcgggc tcgtgaaccc cacgagtggc
     1201 gtcgccacca taggaggccg ccggtactcc gaactcgaga agcccctggc gaccgtcgga
     1261 acggcgctcg aggcgtctga cttccatccc gggcgctcgg cccgcaacca cctccgggtg
     1321 tacgcggcgg ccgcggccat cgctactgcc cgagtcgacg aggtgctcgg catcgtcggg
     1381 ctcaccgagt acgccgatcg ccgggtcggc ggctactcgc tcggcatgcg ccagcgtctg
     1441 ggcctcgcgt tcgcgctact cggcgacccc gacgtgctcg tactcgacga gccgaccaac
     1501 ggcctcgatc cggaggggat ccgctggatg cgggggttcc tccagagtct cgccgccgag
     1561 gggcgcacgg tgctcgtctc ctcgcacctg ctcgccgagg tgcagcagac cgtcgactgg
     1621 gtcgtcatca tctcgcgcgg acgatccgtg tacgagggat ccctcgacgg actcgagacc
     1681 ggccaggtcg tgcggatcga ctcatccgat cgcgcggcgc tcacgaacgc cctcagctct
     1741 gccggcgccg aggtgcgggc gacggatgac gcgctgctgg ccacgggcat caccgcgggt
     1801 gaggccgggg ccgccgccct cgccgccggg gtaccgctca gcctgctcat cacggagtcg
     1861 gtcggtctcg aggaggtttt cctccaactg gtcggatcgg atgccgacag catcgccgac
     1921 ggcatcgcgc aggtgtcggc atgatcggcg cgctgaggtc ggagttcctc aagctgacga
     1981 ccgtgcgact gtggtgggtg ctgttgctca cgctgttcgg gtacgtgggc ttcacgagcg
     2041 gcctgctcgc cggcgtgttc ggcgcgctgg gtgaccagct ggcgacgagc ccgaacgccc
     2101 cgcaactgcc ggcagacgcc cttcctcccc tcatctacag cacggtcacg gcggtgggct
     2161 acgtgatccc gctgattctc ggggcgctct cggtgacctc ggagttccgg taccagacct
     2221 tgacccccac gttcctcgcc ggtccgcgtc ggggcgtcgt gctcggcgcg aaggtcatgg
     2281 ttctggccgc cgccggtgcg gtgttcggca tcgtcgggct ggtgtcatcc atgggcatcg
     2341 gtggttcgat tctcgcgttc agcggaatcg atccgcagct cggcagcgcc gacacctggc
     2401 tgctcgccgg tcgggtcgtc atcgcctcgg cgttgtgggc ggtgatcggg gtgggagtcg
     2461 gcagcctcat caccaatcag gtcgcgtcga tcgtggtcgt gctcgcgttc gcgcagtttg
     2521 tggagccgat cctgcggttg ggcgcatcca tctgggactg gacggcggcg gtcggcaagt
     2581 tccttccggg agcggccagc gacgccctgg tcggctcgag catcttcacc tcgctcggca
     2641 ccggtaccac gtctgttcag accctggagt ggtggcaggg cggcctcgtg ctgctcgggc
     2701 tgggcgtcgt tcttgccctg gccggattcc tgacatcgtg gcgcaaggat gtcacctaga
     2761 ctcgtgcgca tgacgagcgg ggagcgctga tgctggagga ggagtaccag cccaggcgca
     2821 agctgccgcc gcatctggcg ccgaaacctg cccccgagac gcccttcgca tacacgatcc
     2881 gggatgccac ggtcaccgac cttccccacg tgcgcgagat ctacaaccac tacgtcttga
     2941 actcgactgt caccttcgac gagaagccca tgaccctcgc ggacttcaag cgcaagttcg
     3001 ccaaggtgca ggagaagaag atgccgttcc tggtcgcaga gaatccggca ggccagttgc
     3061 tcgggtacgc ctacgtgtat ccgtggaagg agaaggcggc ctaccggtac acggtggaga
     3121 actcgatcta cctcggcccg gcatccaccg gcaaggggct cggcaaggcc ctgctcgccg
     3181 agctgatcga ccggtcgcgt gacgccgggc tcaaggagat catcgccgtg atcgccgaca
     3241 agggcgccga ggcatcgatc gcgatgcacg agaacttcgg attcaaggag atcggccaca
     3301 tgggcaaggt cggcttcaag ttcgaccgct ggctcggcac ggtgctcatg cagaagtcgc
     3361 tcaagtagcg ctcgctcggg taccctccac gatctcccgc agggccttct ccgtctcgtc
     3421 gacagtggac ggttcgccca gccacaggat cctcagcagc aggccgtcga gggcgagggc
     3481 gcgcacccgg gtctgccgcg ggtgctttcc gtggggatcg atcgcgtgtt ccagtgcgcc
     3541 gtaccaggcc tgcgcgtcgg cgcggagcgc gtcgcggcgc atcgcgagga acatgagttc
     3601 gtactgggcg agggcggccc cgcgattctc ggtcgcccac cggtggatga gacgcgcgac
     3661 ggcgcgcagg tcattcattt ctgtgcccgc gaacagggcc acctgctctt cggtcgcgcg
     3721 ccggagtgcg ctgacgtaca ggtcgtcgag gtcgtcgaag tagtaggtcg ccgccgcgag
     3781 cggcaccccc gcctcgcggg cgacggagcg atgcgtgacg gcgccgggcc cctcacgttc
     3841 gagaactcgc agcaccgcgt cgaggagcgc ggcacgacgc gcctcgccct tcggcgtgag
     3901 gctcacgcgg agtgcttccc gccggcctcc agcagcacga cccctccagt caccagcacg
     3961 atccccagca cttgctgcca ggtgagcgac tcgccgaacg cgagccagct gatgaccgcc
     4021 acgagcacga cccccactcc ggcccagatg gcgtacgccg tgccgagggg cactcctcga
     4081 ccgagggcga gcgagagcat cccgaaggcg aagacgtagc cgacgcccac gatggcgtag
     4141 gcccaccaga cggccttctc tcccgaggtg aacttgagga aggtggtggc gatgacctcg
     4201 ccgactatgg caagcccgag gaatacgtaa cccatgatgc aactcctttt ggtacaaatg
     4261 taccagaaag ctacgcacga gtcatcggcc taggctcgtt ccgtgatcac tccacccacc
     4321 caactgcgcc tcgtcgtcga gaccgaggac ttcgacgcag cactcgcctt ctatcgcgac
     4381 gccctcggtc tgcccgaact cgaggcctac gagggggagg gcggcgctcg cgtcgtgatc
     4441 ctctcggtgc cggccgcgac gctggagttg tcgaaccccg cgcaggtgag gctcatcgac
     4501 gaggtcgagg tggggcatcc ggtcgcccgc ccctacccgc tcagcatcag ggtggccttc
     4561 gaggtcgcgg atgccgcggc gacgaccacg gcgctcgagg ccggtggggc gaccgtcgtc
     4621 gccccaccca ccctcacgcc gtggaactcg ctgaactcac gtctggacgc ccccggcgga
     4681 ctgcagatca ctgccttcca ggaactgggg caggtgccgc ccagctagtc acgcgcgcgg
     4741 ggttgcccgc cgcaggatga ggtatcccgc cacgccggag atcgctgtga tcaccagact
     4801 ccaggcatag gtgccgacga agcccgccgc agcgaagaac tcacctaccg caggccagct
     4861 gtcggtgaga gtcagcaggt acagtgccac gatcaccaga gcaccgagcg cgaccaggaa
     4921 gaaggtgact ccggtggcct tccatcgcac gtagaccgcg cccaacaccg cgcccacgaa
     4981 gaagaagaag agcaggagca cgaagaagac gaagaactgc aggtaccacg ggaggtccga
     5041 gccgaagtag gcggcggtga acatcctgcc gcccaacccc catccattgg ttgccttctc
     5101 tatggccgca aggatggtca gaccggtcgc gtacatcgtc gccagaacga cgaactgcac
     5161 tgccgtgccg aggaagaagt ccctccgggt cacgccgtag ccgagggcga acgggaaggt
     5221 gacgttgacg gcctgaatgg ccaccaccat catgtacacg aagatgtaga gcgaggctcc
     5281 gctccactgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctcgggag
     5401 gcgagcccca cgatgacgcc gatcgcggag agctggcccc gcgagagctt cttgatgcgc
     5461 gtgttgaggg ggagccggaa gtcgtcgacg agttgctctg cgaactcggc gtcccagttc
     5521 tcgaagaacc agggggcgct gcgcagcacg tgcttcggcc ggaagtcctc cgggtagcgc
     5581 tggctctcct tgatgaagga gacgtgctgc agtacgcccg cgttctccac cgggctctga
     5641 ccgaacactc ggatggtgcc cgaactggcg aactcctgcc cggtgagcag ttgcatgagc
     5701 gtggtcttgc ctgctccgtt gcgcccgagc aggccgtgga tgcggttgcc ctcgagggtg
     5761 aaggtcgcgt catccaccgc ggcgaggcgg ccgtagcgtc tggtgaggcc gtcgacctcg
     5821 atcactgctg tcatttctct tccttcttga tcatgccgat gagttgtgcg gaggtgatgc
     5881 cgagcttggc tgcctcggtg atgaggggac gcacgaactc ggcttcgaac accgcgcggc
     5941 gctggtcgag gatgcgctgt cgcgctcccg tggccacgaa catgccgata ccgcgcttct
     6001 tgtagaggat ccccgcgtcg accagcaggg tgacgccctt gagcgccgtc gccgggttga
     6061 tgcggtggaa ggcggcgaac tcgttggtcg agggcacctg gtcctcctcg gcgagggcac
     6121 ccatgatgat gtcgttctcg atctgctctg cgacctgcag gaagatcggt cgtgactcgt
     6181 ccatggacct cctctcggtg tatgggttag ttggtcaggt aactaaccaa tcaggccaag
     6241 ctcatcttgt caagctctag gctcgaagaa tgttcgagct gcaccacctc accgccctcg
     6301 agcagctcga ctggctgcgc cggggccagg tctcgccgct cgaactcgcc gagcactacc
     6361 tcgagcgcat cgcacggctc gaccccgaca tcggtgcgtt cgcgaccgtc acggcggatg
     6421 ccgcgcgcgc ccgcgccgcc acactcgagt cgctcgtgcc gcgagccgcc ggactctggg
     6481 ggctgccctc ggccgacaag gacctgtggg cgcgcgccgg ggtgcgcacg acgttcggct
     6541 cgcgccactt ccacgacttc gtgcccgacg agtccgacga gatcgtccag accctcgatg
     6601 gggccggcgc ggtcagcctc ggcaagaccg cgacccccga gttcgggatg cccgcctaca
     6661 ccgagtccgc gctgctgggc gcgacgcgca acccgtggaa ccccgagctc ggcgtcggcg
     6721 gcagcagcgg gggagcggcg gctgccgtcg cggccgggct gcttcctctc gccccgggct
     6781 cagacggcgg cggctcgatc cgcatccccg ccgcggcgac gggtctggtc ggcctcaagc
     6841 cctcccgcgg ccgtgttgcc tccggctcgg gcttcacgac cctcggcggc ctcgtgacct
     6901 acgggccgct cgcccgcacc gtctcggatg cagcgctgct gctcgacgcc ctcatcggcg
     6961 actccgagtg ggcgacccag ccgccgacct gggacggtgg cgcctacctc aatgccgcga
     7021 tccggggcga gggccgctac cagctcgggg tgatgacctc gtcgccgtgg gacgactccc
     7081 acgagatcac cgtctcaccc gaggcgcgcg ccaccctcga cctcgcggtc gccgagctgg
     7141 atgccctcgg tcacggcatc gacaccgtct cgctgccacc gtcgccggac taccccgacg
     7201 ccttccgcac cctgtggcag gcgggggcct cggcgatccc ggtggagggg caggagctcg
     7261 agcggctcga gccgctcacg cggtggcttg tcgagcgcgg tcgcgcgctc gacgcccggc
     7321 ggctcacgga ggcgctcgag taccttgcgg ggttcgagcg cggcgtcatc cgtcagttcg
     7381 ccggcttcga tgccgtgctg accccggcgc tcgcacacac gccaccgccg atcggctggt
     7441 tcgaccagga ggacggcgag cgcaacttcg cgcagcaggt gcagttcaca ccgtggacga
     7501 gcttcgtcaa cgtgagcggg ctgcccgcga tcacgcttcc ggtcggccag agccccgacg
     7561 ggctacccgt cggagtgcag ctggtcgggc gcccgggcgg cgagcacgtg ctgctcgcgc
     7621 tgggcgcgca gctggagcga cggttccggt ggcagcaccg gcatccgtcg atgtggtgac
     7681 gggctacggc accgtcgcga gcatctcgtc gaggatctcc gtcgtcgtcg caacgtacat
     7741 cgcgccgggc acgatgagga agaactcgaa cgcgttgccc tcggtcgcgg agtcgtagag
     7801 ggtgccaccg gcgtaggacg aggtgacata gccgtcgttc gtcccgatga tcagcgagtc
     7861 ggcctcggcc acctgcgagt cggccagcgg ggcatagccg taggcgacca cgacctcgct
     7921 actcgtcgcg taggggcaga cgattccgcc gttgtcctgc atgaggatgt acgggctcga
     7981 cgagacctcg gagcgcacct tggcgaagaa gtcggcgggt gcgaagagcg gccacccgct
     8041 cgtggcgagc cagtcctggc gcgaggcggg gatgatcgag gtgcagtcga tcacgaaggg
     8101 tgccgctgcc gtgggtgtcg ggctcgcggt gggcgtcggc ttgggtttcg cggatgcgct
     8161 cacgctcggg ctgggcgaac cagtcgcggc ggcggtgtcg gtgccgccgg tgggagtgca
     8221 ggccgcgagc agcgcgaccg cgatgcccgc gccgatgagg gtcagtgctg tccgtcgagc
     8281 atgaaagtct ctcatgcgcc taccctagcg gcaggggtcg gcctagcgca ctgggtcggg
     8341 cagggcgaca agagccgccg aacgatccca gagctcggcg gcggccgcgg catcccgagc
     8401 ggtgcgatgc gtgcggccgt tcggcttcag cccgtcgaag tacgttccgc tgggcacgcc
     8461 caccgactcg gcgatggcga gatgcacgag cggcgttgcg ccctgctcgg tgctgatcgc
     8521 caggcgcccg cccgataccc ggttgccgaa gcgcatgaac gccgactcgg ttccgaagtt
     8581 cgtcgcgacg tagccggggt ggaaggcgta ggcctcgacg ctcgatcggc gggccagctc
     8641 ctgagtgaac aggatcacgg cgatcttgct gacgccgtag ggtcgccatc cgcccagata
     8701 cggtcgaccc tgccactgca ggtcggtgag gcggatcggc gagaaggtgt tcgcgacgct
     8761 cgccgtgccg accacccggg catcggaggc ctcgagcacc ggcagcagca gtctcgtcag
     8821 caggtacggc gccaggtggt tgtgctgaat ggtgcgctcg tagccgtcgg ccgacaggcc
     8881 acggctcggc accaggccgc cggcgttgtt ggcgagcaga tcgatgcgct cgtaccgtga
     8941 cgcgagggat gccgcgagcg cgcgcacatc gtcgagcctg tcgtagtccg cgaggaacgc
     9001 cgtaccgccc acgctctccg caacggaccg cgtgcgctcg ggattgcggc ccacgaccgc
     9061 gacctcccag cccgcggcgg cgaggcgggc cgcggcattc gcgccgatgc ccgagctcgc
     9121 gccggtgatg accgccacct tgctcacacg tacccgcccc tcttcagccc cgcttcgatc
     9181 tcgaaccggt tgtgcagtgc gttctggccc gcggagacgt agagcggaat gaaggcgagc
     9241 ccgtaccgct tccactgggc tcgatgcaca gcctcgtgct ccaggatgcc cggcgtgatg
     9301 ttcgtgttgg tgaggtagac ggcgcccacg gtggtaccac cccggccgaa accccagttc
     9361 ggcaggttcg ctgcgacgat cagtccgccc tgacggctca cgtggaagcg tccgagcagg
     9421 gcgccccaga agagggcgca ggcggtcgcg aaccagtagc cgggcctggc caccaccggg
     9481 gtcagcagga gccagcgcag ccatcgcacc cggctgaggc gcgggacgat cccgatgcga
     9541 gcgcgggggt gcggggtgac cggcgggggc ggttcctgcg acatactccg cagaatacag
     9601 tggcaccatg gccggtgtga tccagcgcgt cgcgatggtc tcgatgcaca cctcgccgtc
     9661 ggcggcgccc ggtgtcggag actcgggcgg catgaacgtg gcgattctcg cccttgccgc
     9721 cgaactcgcc atccgcggca cggaagtcga cctcctgacg cgcgcggtcg gggcaccgtc
     9781 gtcccgggtt ctgcttccgg gggtcaccct gcacgaactg cccgcaggct ccggcaccct
     9841 cgtcaagcag gatctcgcga gcgtcaccga cgacttcggc gaggccgtcg cccacctcgc
     9901 caggagcacc cggtacgacg tcatccacag tcactactgg ctcagcggca tcgccgcgct
     9961 ccccgtcgcc ctcgagctgg gcataccgct cgtgcagagc ttccacacgc tcgccgtcat
    10021 gaaggatgcc gatctgggcg ggtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnggcagg gtccaacccc tcaagggcca cgaactcgcg gtccgcgccc tggccgagat
    10201 cagggcgttg cgggggtggg cgccccagtt ggtgatcgtg ggggacgcca cccccgggca
    10261 cgagggattc gtcgaggagc ttggcgcgct ggcgggccag ctcggggtgg ctgatgacat
    10321 ccgcttcgtg gggtcgctgc cgagggagga cctcgccgac ctcctgtccg tcgtcgacct
    10381 cacgatcgtg ccctcgttca gcgagacctt cggtctcgtg gccctcgaat ctgccgcggc
    10441 tggcacgccg gtcatcggct accgcagcgg gggtctgcgc gagtcgatct cggagggggt
    10501 atcggggttg cttctcggta cgcgcgatcc ccggtactgg gccaccgaga tcgccaacct
    10561 tctggagaac gacgagcgcc gactcgagct cggcacctcc gcccgcgcgt tcgcgcagcg
    10621 gttcacctgg ggtgctgccg cgaccggtct gctcggggtg tacgccagcg tcgcgcgctg
    10681 aggcggcggc tcaggccgtg tgcaccggcc gtccgtaggt ctccaggagg cgcagccaga
    10741 tctcgttcat cgtggggtac gccggcaccg catgccacag tcgggcgagc gggacctcgc
    10801 ccgcgaccgc gatcgtcgcc gcgtggatga gctcgctcac gtcctggccc acgaaggtca
    10861 ccccgatgat gaccccgcga tcctcatcga cgaccatccg cgcctgaccc tcgtaatcgt
    10921 cggcctgcag actcgcgccg gccacccagc cgaggttgta gtccacgacc cggatgttcc
    10981 ggcccgcggc cctggccgcc tgcgaggtga tgccgaccga cgccacctcc gggtcactga
    11041 aggtcacctg cggcaccgcg ccgtggtcgg cggtcgcgac gtgggtgccc cagggggcgt
    11101 catccacggg cccgcccttc gcgcgggcgg cgatgacgtc gcccgccgcg cgagcctggt
    11161 acttgccctg gtgggtcagc agcgcgcgat ggttcacatc gccagtcgca tacagccagt
    11221 cgtagccggg cacgcgcagg gtctcgtcga cggtgatcca ctcgccgggc tcaaggccga
    11281 tcgtctccag cccgatgtcg gtcgtgcggg gcgtgcgacc ggtagcgacg agcacctcgc
    11341 tggcggtcac ggaatcatcc ccggcgtgaa ctgtcacacc cgactcggtg cgctcgacga
    11401 gggtggggga ggcccggcgg atgtcgattc cgagtccggc gagccccgcc gtcacgagct
    11461 cggcggcgaa gtcctcctgg ccctggagca ggccgctgcg cgcgaagatc gtcacagctg
    11521 tgccgaagct cgcgtaggcg gtcgccatct cgacggcgac gaccccaccg ccgatgatgg
    11581 ccaggctatc cggcgcgctc gtggcgctcg tcgcgtcccg cggcgtccac gggttagcct
    11641 cgaccagtcc cggaatatcg ggaagcagcg gatcggatcc ggtactgatc acgacggcgt
    11701 gggtcgcaat cacgtcggtg acgctgccgt cagccgcggt caccgacacc cgcttctcgc
    11761 cggtgagtcg cgcgtggcca cgaaggagcg agatgcccgc cccgtcgacc cacgtcacct
    11821 ggctcgcgtc atcccagtcg tgggtgaaga cggtgcgccg ggcgaacacc gctgcgacgt
    11881 cgaggtcgcc ggtgaccgcc tgcttggctc cttgcagtcg ctgggcagcg cgcagggcca
    11941 tgccactgcg gatgagcgcc ttcgatggca tgcacgccca gtaggagcac tcacccccga
    12001 cgagctcgct ctcgatgaga agcgcggtca ggccgccctg cactgcacgg tcggcgacgt
    12061 tctcggcgac ggctccggcc ccgaggatga cgacgtcgta ggtctgcggt tcggtcgtca
    12121 tctcagcgcc ccacgaacgt cgcgggcttg cgggtgacga acgcttccat gccgatgcgg
    12181 gcatcctctg tggcagcgag caggccgagg gcgggctgga gcgttgcctc ggccgcggca
    12241 tccccatcgc ggacggcgat cttggcgttc atgagcgccg cctgcacggc gaggggagcc
    12301 tgggccgcga tgcgctgcgc cagctcgagt ccgcgcgcgt actgctcgcc gtgcggcacg
    12361 acctcctgga cgaggccgat gcgcagcgcc tctgccgcgt cgaacgtgtc gccggtgagg
    12421 atccagcgca tcgcgttgcc ccagccgacg gcgcgcggaa agcggatcgt cgcaccaccg
    12481 aacggcagga tgccccgtgc cacttcgatc tgcgcgaagc tcgccgagtc ggccgcgacc
    12541 acgatgtcgc tggccagcat cagttcgatc ccgagcgtga gacaggtgcc ctgcaccgcc
    12601 atcaccacgg gtttgctgag caactgaccg ttgacctgcc acggatggag cccgccgttc
    12661 ggaacgacgt cgagaccgtc ggggcccagg cgcggcccga cgtcggcgag gtcgagaccc
    12721 cccgtgaagt ggtcgccgtg ggcgaagacg aagcccgccc gcaggtcggg atcgttctcc
    12781 agttcggcgt aggcgatggc cagctgttgg agcatcgcga agtcggcggc gttgcgcttg
    12841 tccgggcggt tcagcccgat cagcagcacc gcgccttcgc gttcgagggt gattcgggaa
    12901 ggtgtctcga ccgcatcagt catgtcccca gactacgtcg cagcacccct gccatgggct
    12961 gcagcgagtc ctctaagctg gtgggcactc gaccgtgaga tccgtgagag aggcctttca
    13021 tgaaccgact cgcccccgca ctcgccgcgg ccgcggtagt cgccgccctg ctcagtggat
    13081 gtaccgcgac ggcgacgtac accgtctcgc ccgaggacct cgcaaccacc gccgagaacg
    13141 cgctcgaaca gcaggtgggt tcccggcccg acatcgactg cggaaccgag gacatcgacc
    13201 tcgtcaaggg cacggtcgtg ccgtgcatcc tgaccgaccc ggcatcgggc gaccggttcg
    13261 atgcaccggt gacgatcgag gaggtcgacg ggtcgaacta cacgatcggc gtgcaggtcg
    13321 cggatgcgcc gaagccctag cgtcgtctag ccggcggccg aggccagcgc gcccaccagg
    13381 cgcaggatcg tcggcatgtc ctcgaccgcg tctcgcggcg aagcgggagc gaactcagtg
    13441 atgccggcac ccgcgagggg gaagcgcgcc accagagcct tgagcgcatt caccagatcg
    13501 gccgagcgca cgccgaaggg ctcgggatag ccgagtccct ggatgtcctc agggtcgagc
    13561 acgtcgaggt cgatatgcac atagacggat gccgcccccg acgccaccac cgcatcgacc
    13621 aggctctggc cgctcacctc gtcggctccg atggtcacca cacggtgcgc ggcgatgtac
    13681 tcgtcctcgc ccgggtcgaa cgaccgcgca ccggcgagga tcacgcggct gggcttcagg
    13741 ggcgtgccgg gcacgagctg ggccggcccc tcgccgagca gggttcgcag caccatcccg
    13801 gtgaacgcgc ccgagggact cgactccggc gtgttgaggt cggggtgggc atccaaccag
    13861 atgactgcca cgtcgtcgcc cgccacgtgc tcgatggccg caagctctac gccgcagtcg
    13921 ccgccgatgg tgatcaccgg gccgtcgatg gtggccaggt cggcggcgag acggtcgcga
    13981 acgagtgcga tcgagctgag gcgatgcacc gccgtgccct ggccgtcacc cgcctcgagg
    14041 ggaatctcta tggttctggt ggaactgctg ggcagatccc cccggatcgc ctcggcccca
    14101 tcaacgagcc gcatcgcccg ggacgagccc gagccctgcc actggggaat gacgacgaag
    14161 gtggcgacca tggggaaacc ctagcgcgtg gcgattgcga gattcgcgcg cagttcgtcc
    14221 gcgttctgac gcactccgta cgcgggctgg ttggtctcca cccgccagct gtcggcgagc
    14281 ccgcccacga ccaccgcgtc gaatccgaac tggtcgtaga gcttctgcgc gaactccagc
    14341 gcctcggggt cgtcgccggc gagagccagc gcgcgacggt tcgatgtgcc ggcgggcgtg
    14401 ccgtcagtcg cgatgctcgc cgcctggatg tggttgaacg ccttcaccac gcggctcgtt
    14461 ggcaggtgct tctgcagcag tccgctggag gtgtcggtct tctcgtcgag cgcggcgatg
    14521 ttgccatccc gctgcgggta gtagttgttg gtgtcgagca cgatcttgcc cgcgagcggt
    14581 tcgaccggaa cggaggcata cgcgcgcagt ggcaccgtca ccatgacgac atcgccgctc
    14641 gccgcggcat ccgcaaccgt tgcggcgcgc gccttcgggc cgagttcggc gacgagggcg
    14701 gccagcgtct ccggcccgcg ggagttggag atgactacgt cgtagccgtt gtcgatcgcc
    14761 ttgcgtgcca cgtggctgcc gatgtttccg gcgccgatga ttccgagagt tgtcatgctg
    14821 gtgtcaagcg cgacgccgcg cgctttgttc ccgcactcgg ctcgttgcta ggaggtgaca
    14881 gccttcggtg cgctaccggc cttgagggcg gccagacggg catccacctc ggcctgcttg
    14941 tcgaggtcgt cgaggctctc gaactgagcg tcgagggtgg atgccgcgat ctcctgctga
    15001 ccgagcacct tcgcctcctc gcgccggatc ttgtcctcga agcgcgagat ctcgctggtc
    15061 gggtcgagca gatcgatcga cttcacggcg tcgatgacct gggactgggc tgccgccgac
    15121 ttggcccgag cgacgagctc gtcgcgcttg gcggtgagtt cgctcagctt cgcgcgcatg
    15181 ccgtcgaggc cgctcttgag ctgctcgacg acggtggtct gcgacgcgat cgtgggctcg
    15241 acggccttcg cttcgttctc cgaggagacc tgcttgccga gggcgatctt ggccaggttg
    15301 tcgaacttgt cggcgtcggc cgagttgccg ccggcacgta gctcgtcggc cttggagctg
    15361 gccgcgaggg ccttgcgacc ccagtcggcg gccgcgtcga tgtcctcggc gtggtcctgc
    15421 tccatcaggc gcaggttgcc gatggtctgc gccacggcgc tctccgcctc ggcgatcgag
    15481 ttcgtgtagt cgcgcaccag ctggtcgagc agcagctgcg ggtcctccgc ctgatcgatg
    15541 agggcgttga tgttggcctt cagaagctgg gagatgcgcc cgaggattga ttgcttagcc
    15601 atgtcagcgt gcctttcgtg tggagaacct tcttcgaggt ctgtgttaga acctaccgcc
    15661 gcggctacgc ccgccaccac tgctggagcg cgcgctgcgt gaacttccgc cgaacgaacc
    15721 ggagcgcgaa ctgcggctcg gccggtagct gctgcccgac gacccaccgc caccgaacca
    15781 gccaccaccg cccgaactgc ggccgccgcc tcccgatccg agcatgccgc cgatgacacc
    15841 gccgagaagc ccgccgaggt cggcgccgtc ggatccccgc ggcatgccgc cgtagctgtc
    15901 ctgccgagtg aactccgaga catcgctgcg ggccagatcg aacgcctgtg aggcgaggtc
    15961 gttggctcgc tgcgcctcac cgagtgcctt caccggatcg gagtcggaca gtgcttcggc
    16021 ctggccgaga tgacgctccg cctcgctcac ccgggtgcgg gcgctctcgc cgataccgcc
    16081 ccgtcgggtg gtgatgtact cccgggcgct cgacaactgg gcgcgtgcgg cggagatcgc
    16141 ggcatccagc tgcgtgcgtg ccgaggagat cttctgctgt tcgtcgcgca ctccggtgaa
    16201 gacctggtcg agggctgagt tggccagttc cagcttcgcg aggcttgcca cggggtcgcc
    16261 cttcgtggcg gttgccccgc tcagtgccgc agccgcggcg gcgatcgccg gtgcgagggc
    16321 ggcggatgcc tcgtcctgcg gcagcgcacg ggccgccgca acgtcctggg tggtgtcggc
    16381 gatcgccgcc tcgagcttcg tcgtcgcatc cccgagggtg gacttcaggg tgtcgatcgc
    16441 cgtgaacagc tgctgggcct ggcccacgct ggcctgcacc gtgcgcaccg caatggccgc
    16501 ctggctcgaa tcgctcgcgg ctatcgcggt gtcggccttg cctgctgccg cctcggcgaa
    16561 gtcgagcagt ttggccgcct gctcgatgtt ggtcgagacc gctgcgacgg cggatgccgc
    16621 gtacatcttc gagagcgcct tcacggtgtc tgcggcctct gccgaccggg ctcgcgccgc
    16681 accgatggag tcgcgggccg ccgcgagcgc ctgcggcacg ttcttctcga gttcgcgcag
    16741 tgcgtcgaag gcgtcggcct ggctgtcgag ttccgcatcg gcggcctcac acagctcgat
    16801 gatcttcgtg gtccacgccc tctggtcggc gggggagtcg ggtgtcgcgt cgtcgagctt
    16861 ctgcttgagg gtgaacgcct cggcgacctt ggccttggcg ctggccagca ccgctgtgaa
    16921 gtccttggtc gcgtcatccc cgaattgggc aacggcgaag ccgagttcct gctcgctggt
    16981 cttgagggag tcgtcaagct gaacgagcag gctgccggcc cgctggtcga gctgcttctg
    17041 ggtcatgcct tcgggcgcgg agaccacccg gccgtcacta ccgcgccgac ggatgcgcga
    17101 gaagatgaac accccggcgc cgaccaccgc gaccccaccc acgatcggca ggatcgggat
    17161 gccgccgccg gactccgccg gcggatcggt gggggttgcc gggtcagtcg gctggttcgg
    17221 accgaccaca ccggtcgcct ccgcctcgag cgactgtgcc cccgcgatcg cggcctgcgc
    17281 ccagttgtcg tcgcgcaact cggcctcaat ggcctgctcg acgacggtga tctgtgtgtc
    17341 ggagagcgtg aagctgtcat cgatcgagag agcgtactgg cggtcgtcga ccgcgaccgc
    17401 gagcagcaca tcattgccgc ccaggccgtt gtcgatcgcc acggtgtcgg cccagtcgac
    17461 ggggtcgctg gggttggaga acgagtccac gtagacgacg aagagctgga tgctcgccct
    17521 cgtgtacagg gagtccaggg ccccgaggat ctcggtgccc tcgtccgggg tgatcgcgcg
    17581 caccttgtcg acgatgtacg cgccgctcag gtcaacgggg tcctcggcga gggcggcagg
    17641 ggttccgagg gcgagcaacc cggcaagaat cgcggtgcca acaccgattc gtgaccacat
    17701 cagctgcgac tcctctgctt gcccgacgaa gggattctat tggaggtgct cccgcgtgcc
    17761 gaaggcccga tttgtacccc ggacagtctt cggctaggtt ctcactcact aaaggaagat
    17821 cgccgtcaca acgaagtggc ggcgattttc ttttgccgct ctactgcagt tggatcgtcg
    17881 accgcgggcc cgtcaccagg tccagacctg gcgtcgagcc gtcggttggt gcggcatcgg
    17941 gcaggaagtc cagagccgcc gacaggtcgt agtcaccggc gggaaccggc ggcaggtcgg
    18001 cccggaactg ctcggagccg tcatcctcca cggtgcactg caccggcacg aaggttgcct
    18061 cgtactcgat cgactcgccc ggggccaggg tcacggcgcg ttccggggtc tccgcctgac
    18121 cgttgctgtg ccagagcacg acgccgtcgc gcgacagcgt gatggcgggc accgtggtgg
    18181 tggtgccgga cactgtgagg tcgctggtgt tcgtgaggcg cacggcgccg accacggggt
    18241 cggtgcctac cggcgctgtg gcagggaagg cgacatccag ctgcagaccc agagtgctcg
    18301 gcgccacgtc ggccaggggc gcttcgcaga ggttgatcct gtcggccggc gctcgcttga
    18361 tcgagtcggc actcgcctcg ggtgcggcgc cgccctcggc ggactggtcc atcatcgaga
    18421 ccgtgctggg cggctgggag accagagagg tctggatgac gacgacgctg attcccgcga
    18481 cggcgagcac gctcacggcc cccgccgcga gctggcgcgg tagccgacgc cgacggctgc
    18541 gcgcgatgat gcgctgggca tccagctcat ggggcgcgtg ggccgatgag agcaggtcgc
    18601 gcagttcgga ttcactgggc acggggcgcc ccctttccgt cgcggatggc agggccgtct
    18661 tcggtcaggg tgacggcgag cttggccagc gcgtcgctga ggtagcgctt caccgcgccg
    18721 gcactgattc cgagccactc ggcgatgtcg tcgaccttga ggtcctcgta gtagcgcagt
    18781 acgacgcagg cgcgctcgcg cggcgtgagc ttcatcaact cgcgagtcac gtccagtcgc
    18841 gactcggtct gcgcgtcgcg gggttcgtcc agttcgggag cggcgttcag gtgggcgatc
    18901 ttgcgccacc ggctggtgcg ccgacccgag tcaagatagg tgttcaggat ggcccggcgc
    18961 acgtacgcct cggcgctcgc gatcgagaat ccgttgcgca ccctgccgaa ggtcttgacc
    19021 agggcgtcct gcacgaggtc ggacgcgtca tcccggttgc cggagatcag gaatgcgtag
    19081 cgtgtcagcg cgtcgccgcg ctcggcgacg aggcgtgtga ccacgtcctc ccagcgcgtc
    19141 ggctcgccgg ctgtgctgga cacgcctgac ctcctcgcgc tgacggatgc cgcggcgcgc
    19201 tgcgcccggg ccatctcaaa agtagtggag ctcacacctt ctaagacgcg caacaagcgc
    19261 gaaacgttgg gccagacccg acccggagcg cgaaacggtg agtcaggcga gtttgctgcg
    19321 caggatgtcg agctgcttgg tgaccgcggc aggcacgcgc ccgtcgaagc tctcgaagaa
    19381 ctcggacgtc gcatccgcct cggccaacca ggtctcgcgg tcgatggcga acagctcgga
    19441 caggtcgtcg tcggagatat ccaggccatc gacgttcagc tcgcccgcgt gggggagcag
    19501 cccgatcggg ctctcctctg cggcgacctg gccgtcgacg cgctcgagga tccactcgat
    19561 gacccgcgag ttctcgccga agccgggcca caggaaccga ccgtcggcac ccttgcggaa
    19621 ccagttgacc tggaagatgc gcggcaccga tccggtcttg cgcagctcgg cacccacgct
    19681 gagccagtgg gcccagtgat cggccatgtt gtacccgcag aacggggcca tggcgaaggg
    19741 gtcgcggcga agctccccca gagtgccttc ggcggccgcg gtgcgctcgg acgagatcgt
    19801 ggcgcccagg tagacaccat gctcccaatc ccgtgcctcc accactagcg gcacgttggt
    19861 cgcgcggcgg ccgccgaaga ttatcgcgtc gatgacgacg ccccgtgggt cctcccagtc
    19921 atccgagatg ctcgggcact ggcgggctgc cacagtaaaa cgcgagttcg ggtgggctgc
    19981 agggcgaccc gatgccggtg tccacgggtt gccctcccag tcggtgagct gggcgggaac
    20041 cgtgtcgctc atgccctccc accagacgtc gccatcgtcg cggagggcga cgttcgtgaa
    20101 gatcgtgttg ccccagatgg tgtccatcgc ggtggcgttg gtcgagtagc cggtgcccgg
    20161 cgcgactccg aagaagccgg cctcggggtt gatcgcgcgc aggcgtccat cctcaccgcg
    20221 gcccagccag gcgatgtcgt cgccgatggt ctcgaccttc cagccgggaa tggtcggctt
    20281 cagcatggcg aggttcgtct tcccgcaggc gctcgggaac gccgcggcca cgtggaagac
    20341 ccgaccccgc gggttggtga ccttgatgat gagcatgtgc tcggcgagcc atccctcgtc
    20401 gcgggccatg accgaggcga tgcgcagcgc gaaagacttc ttgccgagca gcgcgttgcc
    20461 gccgtaaccc gaaccgaaag accagatctc gcggctctca gggaagtggg tgatgtactt
    20521 cgtctcgttg cacggccacg gcacatcgtc ctggccgggg gagagcggcg ctcccacgct
    20581 gtgcacggca cgcacccact gggtcgtggg agtgaactgg ctcagcacct cggtgccgat
    20641 gcgggtcatg attcccatgc tcgcggcgac gtagggcgaa tccgtgactt cgaccccaag
    20701 gcgcgacagc ggggatccca gtgggcccat ggagaacggc accacgtaca tcgtgcgacc
    20761 gcgcatactt ccgctgaaca gcggctgcag ggtggcgcgc atctcgatcg ggtccatcca
    20821 gttgttggtg gggccggcat ccacgggatc gaccgagcag atgaaggtgc ggtcctcgac
    20881 ccgtgcgacg tcgttgggat cgctgcgagc gaggaagctg taagggcggt gctcggcgtt
    20941 gaggcggatc agggttccgc ttgcgaccat ctcgcgggcc agggcatccc actccgcgcg
    21001 ggaaccgtcg caccagacga cgtcgtcggg cagcgtcagg gctgcgatct cgtcgaccca
    21061 ggcgacgacc gactccggcg caccatcggg cacgggggac tgagtacggg tgatctccgc
    21121 gatgctcatc tagcattcct tccggctcaa aacgtggtgt tcttccactg tgcgccatgc
    21181 aatgcggctc aatctctgaa atgtgggtga aagatcactc gatctttcgc taatctggtc
    21241 tcgtgaacga tcttgtgacc ctcggacagc gcattcggca cttccgcacc tcggccggca
    21301 tgacccttga ccagctcggc gacaaggtcg gcatcgccgg cagtcagctc tcgctcatgg
    21361 agaacggccg acgggaacca cggctgagcc tgctcggcgc gatcgccacg gccacgggca
    21421 tccagctcgc ggacctgctg gatgacgcgc cccccagcaa gcgcgcagcc ctcgagatcg
    21481 agctggaccg ggcacagcgc tcaagtgtgt acgcgtcgct cggacttccc tcgatccgcc
    21541 cgtcgaaggg caccgccgat gagaccctcg aggccctggt gggcctccac cgcgagctcg
    21601 cccggcgcgc gcgcgaggcg atcgcgacgc ccgaggaggc gaggcgcgcc aacaccgacc
    21661 tgcgcctgca tatgcgcacg ctcgacaact acctgcccga catcgacgac gtcgccgaga
    21721 agctgctggc cgatgtcgga cacaccacgg gcgccctcac tcaccgctcg gtgagcatga
    21781 tggctgagcg cctcggcttc gagctcgtgc acgtcaacga cctgccgcac tcggcccgct
    21841 cggtcaccga tctcgccaac gggcgcatct accttccgcc ggcgtcgatc ccgggaggcc
    21901 acggtctgcg cggcatggca ctgcaggcga tggcgcatcg tgtgctcggc cacgagacac
    21961 ccgcgaccta tgccgacttc ctgcgccaac gactggagat caactacttc gcggcggcgg
    22021 tgctcatgcc gaggcgtcag tccgtcgagt tcctctcggc ggccaaagcc gaccgcaaca
    22081 tcgccgtcga ggacttcagg gatgccttcg gcgtcaccca cgaggctgcc gcgctgcgct
    22141 tcacgaacct cgccacagca cacctcgatc tcgaggtgca cttcctgcgc gtcggagacg
    22201 acggcgccgt gtacaaggcc tacgagaacg acggcctgcg tctgcccgtg gacgtgaccg
    22261 gatcgacaga ggggagatcg tctgccgcaa gtggagtgcg cgcacggcgt tcgcccgcac
    22321 caaccgcacg accgagttct accagtacac cgacaccccg gagggcacct tcttcgactc
    22381 gacccagacg ggaaccacgt cggccgagga gttctccatc acgatcggcg ttccgttctc
    22441 gcactcgaag tggttccgcg gtcgcgagac gagcaaccgc acttcgtcga cctgccccga
    22501 cgagtcatgc tgccgcacgc ccgacggcac cctcaccgag cgctgggcgg gcaaggcctg
    22561 gtcgagtgcg cggctgcacg cgcacatcct gtcgccgttg ccgtcgggga ccttccccgg
    22621 ggtcgacgat cgcgagctct acgagttcct cgaggcgcac gccgtcacga acgactgaac
    22681 tcgcgcgccg cgagggtgcc cgcttcccgg agccagcgcc gagggttcgc cgttgctgcc
    22741 tccacgctgc cagccagatc ggtgagtgac gccgaccaac caggcggggc cgcggcgagc
    22801 gaccccgcga tgacggcgac gggtcggccc aggccgagcg cgtggcccac gaccttgccg
    22861 cccagcgatt ggtcgtcgaa ccgcccctcg cccgtgatga tcagatccgc ggatgccgcc
    22921 gccgccgtca acccggtgag ctcggcgatc cgcggcgcac cgggcacgat tcgcgcaccc
    22981 cacaccgcgg cgaacccgta ggccgtcccg cctgcggctc ccgcacccgc agcgtcgggg
    23041 tcgcccccga gcacctgcgc ccaccgcgcc agcgcggcat cgagctcggc gcactgggtg
    23101 gggctcgcgc ccttctgcgg gccgaagacg tgtgcggcgc cggtgggccc gagcagggga
    23161 ttggtcacat cggtgagcag ggtcaccccg ccgggcggag ccggcacgag aaggccgcga
    23221 tcgactcgcg ccagcgtcgc gaggccagcg ccgccctcgg cgatcgggcg gtcgcggtcg
    23281 tcggtcagtc ccaaccccag cgcggacagc gcgccagaac ctccgtcggt cgtcgccgag
    23341 ccacccagcg cgagcacgag tgagtgtgca cccgcctgaa gggccaggcg gatgacctcg
    23401 ccggtgccgc gcgtcgacgc ggccggggca tcccgaaccg tcatgagcgg caggccactg
    23461 gccccggcga gctcgaccac cgccgtgccg tcgggcagct ccagccactc gcccggcgtc
    23521 ggccgaccgt ctgggccggt caccgcgccg gcgctgcgtc ttaccgcacc tggcaccgac
    23581 gcctcgatcg cgtcgagggt tccctcaccg ccgtcggcct ggggcagaag tgtcagcacg
    23641 tcgcccgggc gttccgacag ccagcccgcc gcgatggcgt cggcgacatc ctccgcggcc
    23701 agcgatccct tgaaggagtc gggcgcgatc acaacgcgca cgatggaccg ttctgagctg
    23761 cccatcatgg cgtcagagta gcagcggagc ggtgctgtgc cgagaggtgt tcacggttgt
    23821 agcctgtgcg tgacgtccgg caacgatgtc ggcgactgat caggagagtg acccaaccca
    23881 tgtcaacaac caccgagaac cagctcgtcg gactgttccg tcagtcgcgc accgtctggg
    23941 cgattcgcgc cgcggccgcg ctcatcatcg gcatcctcgt gctggtgtgg ccgcaggcca
    24001 cagtgagcat cctcgcgatc ctcctgggca tctacttcgt gatcctcggc gtcgtgcgcg
    24061 tcatccaggg attcgtcgac aaggacctca acggcggcgg caaggctgcc aacttcatca
    24121 tcggtgcgct gatcctcgcg gccggcgtga tcgtcatcaa gaaccccttc gagaccgccg
    24181 tgttcatcgt gctgctcgtc ggcatctcgt ggatcttcga gggcatcgcg acgctggtcg
    24241 acaccgctcg cgggcacggt gacggcgtct cgatcctcat cggaatcctc atcgccctgg
    24301 ccggtgtggt cgtcgtgctg ttcgccccgg ccgcaaccac cgcctacgcg gtcttcttcg
    24361 gcatcgtgct gatcatcgtg gcgatcctcg acttcgtgct gctgttcgcg gtcggtcggg
    24421 cggtcaagaa ggcgaaggcc tgatcatggc cgagagcaag aactccaccg gcgccgtcgt
    24481 caagctcgtc gtctccatcg cggcgatcgt ggtcggactc ttcctggtgt tcggcgccac
    24541 gacccccgct atcgccgacg gcgtcttcgt cggctcgatc tcactcgtgc tggcgatcta
    24601 cctctggtcg ggtgtgacca cgggtcaggc gcgcaccctc gcgatcgtgc tcatcctgct
    24661 ggccgcctat gcgttcattc gtggattcgg tctcctcgac ctcgcggtac tgcgccagct
    24721 cggcggaatc gcggcgatcg tcatgggcgt catcctgatc atcccgttcc tgcgtgagcg
    24781 cttcgggagc aagtcc
//
LOCUS       DAOSOU010000005        23416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2196_length_23416_cov_6.059801, whole genome shotgun sequence.
ACCESSION   DAOSOU010000005 DAOSOU010000000
VERSION     DAOSOU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 23416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23416
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2196_length_23416_cov_6.059801"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..492)
                     /locus_tag="PLW15_00600"
     CDS             complement(175..492)
                     /locus_tag="PLW15_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32929.1"
                     /translation="MFTALTVATLASHLGGPGFGFGFLLLFLIPLFFFGLFVLFASIG
                     RRRWMRAGGYGPFGHGHGGWGSGARSAESTLAERFAQGDIDEKEYRARLEVLRASNPP
                     VAR"
     gene            complement(621..1286)
                     /locus_tag="PLW15_00605"
     CDS             complement(621..1286)
                     /locus_tag="PLW15_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT32930.1"
                     /translation="MIRVAIADDQQLIRAGFRSLLEAEPDIEIVGEAGSGSEAVSLAV
                     ATRPDVMLMDIRMPDGDGLWATEQIVGNPDLAQTHIVIVTTFELDEYVGRAIRAGASG
                     FLVKDTEPDEFIRAVHVVAAGDALLSPTVTRRLLERLSSELRETPDASQLSVITDRER
                     EVLALVGQGLTNEEIGKQLFLSPLTAKTHVSRIMAKLAARDRVQLVVIAYETGLVSPG
                     RTE"
     gene            complement(1283..2398)
                     /locus_tag="PLW15_00610"
     CDS             complement(1283..2398)
                     /locus_tag="PLW15_00610"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT32931.1"
                     /translation="MRLLLPVFVSLAIQVPPSLYWILFQRQHSPSVLIAAALSVIGPL
                     LLLAARRFPGPVVALVTAASCVDLVLSISQPGPPFIALAFAIGGAVLRGARLWAWISV
                     GTGWLVTLVVGFMVGLDWRPAQIAITTLGILIVFGLAEGMRTRREHYEEYRREYTQRK
                     QSEVQAERVRIARELHDVLAHSLSQINVQASVGLHLMDKQPEKASEALASIKETSKTA
                     LDEVRSVLGVLRAEDGRDPTAPLVPEPDLSRLTGLAASVTSQGVEVTLSNSLDAAPQA
                     VQLAIYRIVQEALTNVVRHARATRATVDISEADGSYLIAVADNGVGLSDGRDITTGRG
                     LLGMRERAELLGGTLEVGPGPRGGLLVMVAIPQKEHA"
     gene            complement(2686..2758)
                     /locus_tag="PLW15_00615"
     tRNA            complement(2686..2758)
                     /locus_tag="PLW15_00615"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2723..2725),aa:Asn,seq:gtt)
     gene            complement(2827..3825)
                     /locus_tag="PLW15_00620"
     CDS             complement(2827..3825)
                     /locus_tag="PLW15_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HOT32932.1"
                     /translation="MTRRTPGEHRDLVAPSAAPLTRPQPGPIAILPEPRASFVAAVES
                     AGGVVAPLSDETRGLIWLAPAETPELVDLLQSHPGIGWVQLPWAGVDAFASTLKRFAG
                     SSSPLWTSAKGSYSEPVAEHALALTLAVLRGLPEKARATSWAPVKVGTSLYGRHVLVV
                     GAGGIAVEVMRLFAPFEVEFTIVRRSADPLPGAQRTVPVADLLSVLPDADVVILAAAA
                     TDETAQLIGATELAAMKANAALINIARGALVDQEALLASLNSGHLAGAGIDVTDPEPL
                     PDGHPLWQAPRCIVTGHTADTPEMTEPLLAERVRRNVEALLGDGRFVGVVDPAAGY"
     gene            complement(3822..4628)
                     /locus_tag="PLW15_00625"
     CDS             complement(3822..4628)
                     /locus_tag="PLW15_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT32933.1"
                     /translation="MSAVVVQDLSLRYPSENPDTRSVAVNGVSFTIEPGEVLAVVGET
                     GSGKSTLARAIALEADLSAADSPVISGGALTVLGTPVRGISARKRDRLQMQVGYLRQE
                     AGDFLEPRLTVGENVAEPIFSRDRRFDQDEAGYAVATLIDAVRLPLGTMNKYPHELSK
                     GQRQRVAIAKALILEPTLLIADDPTAGIDVTVRSAVLDILVHLQKQRAFSALIVTADL
                     AEVRRVSTQVAIMHGGIFVGLGQLEEVLADPRHPYVAGLARSLDHLQDAG"
     gene            complement(4625..6271)
                     /locus_tag="PLW15_00630"
     CDS             complement(4625..6271)
                     /locus_tag="PLW15_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT32934.1"
                     /translation="MKTVVDIRNLEVTFATDGGDIPAVDDVSLTVDSGEVVAIVGESG
                     SGKSVTAKSIIGLLPETAVTRGVILLNGRDVVGLTGEKLRATRGLDAAMVFQEPSTSL
                     NPVYTVGWQLAEGLRAHGKYSRRQARAKAIEYLGKVGIPEPEKRIDHYPHQFSGGQKQ
                     RVVIAMALALDPELIIADEPTTALDVTVQAEILDLLRRCRDEFGTAIILITHNMGVVA
                     DLADRVVVMYEGKVVEQATATKLFGSPQHPYTQKLLAAVPRIDTTRERTPVAEAPPVV
                     QATDLVIEYPGRFGRGGFRAVDRVSFTIAPGEVLGLVGESGSGKTTIGRAIGGLTKIS
                     GGSLQVLGHEMLTVRERGFKPVREQIGFVFQDPASSFNPLLTIAQCVAEPLIVHGRAG
                     DATAARPRVDELLEAVQLPRAFGDRYPHELSGGQRQRASLARALALEPTLLIADEPTS
                     ALDVSVQARVLELFAELQNQFGFAALFISHDLAVVDTVSDRIAVLYRGELVEEGPPSQ
                     VLGAPQHPYTQRLLASLPVPDPAEQAVRREELRALTRGVQ"
     gene            complement(6268..7224)
                     /locus_tag="PLW15_00635"
     CDS             complement(6268..7224)
                     /locus_tag="PLW15_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32935.1"
                     /translation="MTKILDRLPVVRQVRQSVGLQRGMLVTGLAITAVFVLTAIFAPL
                     LAPYGYSQLRDASGTFGAQLPPGGAHLLGTTVGGYDVLSRVIWGTQTAVIVIIVAVLL
                     SIFAGVALGLISGYIGGWLDRVLVVIADAIYAFPSLLLAIVAAIAISGGQSSFWGGIL
                     AAAISITFVFIPQYFRVIRAETVRIKSEAYVESAKVIGSSAPRIMVQHVLRNATRTLP
                     LIFTLNSSEAILTLAGLGFLGFGIEPTAAAEWGYDLNKSLSDVTSGIWWTSLFPGLAI
                     VLSVLGITLVGESLNDLADPRLRTRRRAEVAAHTPDPLEVQS"
     gene            complement(7224..8312)
                     /locus_tag="PLW15_00640"
     CDS             complement(7224..8312)
                     /locus_tag="PLW15_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32936.1"
                     /translation="MTAPEIAGPASVVPTTRPGTGGNKLLRFIIVRFLLIFPTIFILV
                     TLVFVLMRTIGDPITAALGGRLPADQLAQRIEEAGYNRPILVQYFEYIGQVFTGNFGE
                     TISDHRPVVDVLVRYGGATLELAFYALIVAFIVGIPLGMLAAYVRDRWPDAILRVFAI
                     LCYATPVFFAGLLLKLIFAVGLGWLPVAGRASVRTELQLTRVDNPTGIYLIDALKTSN
                     PGAVYADLLAHAVLPGVALGLLTAGIFLRLVRTNVIGTLSMDYVDSARSRGVREYRLV
                     TKHALKPASIPIITVIGLQIAVLLGGAVLTETTFEWQGLGFMLTQYLTARDFVAVQGI
                     VALLAVIVALTNFIVDIIAALIDPRVRY"
     gene            complement(8410..10023)
                     /locus_tag="PLW15_00645"
     CDS             complement(8410..10023)
                     /locus_tag="PLW15_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT32937.1"
                     /translation="MTSASTKRALGVLAGTAAFALALTGCTASGGGTDDGAILVGTTD
                     KVVTLDPAGSYDNGSFAVQNQIYAFLLNSPLGSPDPQPDLAESAEFTAPNDYTVVLKP
                     DLKFANGHDLTASDVKFTFDRQLAIAAPDGPSSLLYNLDSTEAVDDTTVVFHLKAAND
                     QVFPQILTTFPGAVVDEEVFSATELTSDDDIVAGKAFNGQYEIASYDFNKTIEFVPNP
                     NYAGLLGPAQNSSVILSYYADSSNLKLDVQQGNIDVAYRSLSATDVADLRADDNVTVH
                     DGPGGEIRYIVFNFNTQPYGATTAEADPAKAQAVRQAVASLVDRQEIADQVYKGTYTP
                     LYSFVPDGFTGATTVLKDLYGDGSGGPDATKAAAILAAAGVTTPVALSLQYNPDHYGP
                     SSGDEYALVKSQLEADGLFTVDLQSTEWVQYSEDRVNDVYPAYQLGWFPDYSDADNYL
                     TPFFLTNNFLSNHYSNPTVDDLITQQGVTQDPAERTAIIEQIQAVVGEDLSTVPLLQG
                     AQVAVSGKTIDGLTLDASFKFRFATLVKG"
     gene            complement(10216..12078)
                     /gene="dnaG"
                     /locus_tag="PLW15_00650"
     CDS             complement(10216..12078)
                     /gene="dnaG"
                     /locus_tag="PLW15_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT32938.1"
                     /translation="MAGLIKRSDIDEVRSRTNLADIVGDYVSLKSAGVGSMKGLCPFH
                     EERSPSFHVRPQVGFYHCFGCGEGGDVFTFLQKMDHVSFSEAVERMAARIGYELHYED
                     GGAATDHGVRARILAANQAAAEFYVAQLTTDEAEPARRFLGERGFDSSAAQRFGLGFA
                     PRGGALGRSLAALGYTEEELISAGLLGRGDRGDVYDRFRGRLIWPIRDATGATLGFGA
                     RKLLDDDGGPKYLNTPETVVYHKSQVLYGLDLAKRDIARGRQVVVVEGYTDVMACHLA
                     GVTTAVATCGTAFGVDHIKLVRRMIGDVDNADTTGTGEVIFTFDPDEAGQKAASRAFA
                     EEHRFAAQTFVAVAPDGLDPCDLRLNRGDDAVRRLVKTRRPLFEFKIRRELAAHDLET
                     VEGRVAALRGAAPVVASIRDQALSVGYVRNLAGWLGMDPGEVGRAVASVRGASARASE
                     PDRSSVQEQPAAEALDLRKLPTDPATRLERDALMAMLQYPSEVGADLVRRASSVDFVD
                     NTLSVVRDAIATSIEHIASPDWLSRVAAEVPAPFETLVKQLGVAPIPERAELIGVYCR
                     GVSAALVDRDLLRQKRDLLGTMQRIDSVAEPERYAQVQRDLVRVEAERRALRDE"
     gene            complement(12121..13365)
                     /locus_tag="PLW15_00655"
     CDS             complement(12121..13365)
                     /locus_tag="PLW15_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOT32939.1"
                     /translation="MADSSEYSAADRERWLPEEHSSRRSDFARDRGRVLHSSALRRLA
                     AKTQVLSPTAGLDFARNRLTHSLEVAQVGRELADSLGLDPDVVDTACLAHDLGHPPFG
                     HNGERALNDWSADIGGFEGNAQTLRVLTRIEPKVFGDDDRSYGLNLTRASLDASCKYP
                     WPAAQGVPDPSGRSKYGFYDDDTEVFQWLRAGAPDRRRCIEAQVMDLSDDIGYSVHDF
                     EDAIVNGYVDVTALGARVDHDDLVNSMFEWIGGEVTHDELIAAFDRLDSLDFWIDRWD
                     GSRQAHGRLKNLTSQLIGRFAGAATQATRAAHSGVLSRFGADVVVPREIQAEIAVLKG
                     IVAAFVMSTNARRPIYAQQRDILSELADVLLWTGPAHLDVGFRADWDAAATDQQRKRA
                     VVDQVASLTDQSAMAWHERLVG"
     gene            complement(13355..>13486)
                     /locus_tag="PLW15_00660"
     CDS             complement(13355..>13486)
                     /locus_tag="PLW15_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT32940.1"
                     /translation="EGQRGRAGTPKSPSLPQGWLDSRELQETHRAELTEAELSTSGG"
     assembly_gap    13487..13586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13587..14320)
                     /gene="dusB"
                     /locus_tag="PLW15_00665"
     CDS             complement(<13587..14320)
                     /gene="dusB"
                     /locus_tag="PLW15_00665"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT32941.1"
                     /translation="MTILADAQTSAAPTLHIGPIALDVPVVLAPMAGITNTAFRRLCR
                     EYGAGLYVSEMITSRALVERTPESMRLIRHHESETTRSIQLYGVDPKTVSEAVTMLVA
                     EDRADHIDLNFGCPVPKVTRKGGGAALPWKRDLFREIVERAVKAAGDIPLTVKMRKGI
                     DSDHLTYLEAARAASGAGVASIALHARTAAEFYSGTADWSAIEKLKNTITDTPILGNG
                     DIWSADDALRMVDETGCDGVVVGRGCL"
     gene            14348..14815
                     /locus_tag="PLW15_00670"
     CDS             14348..14815
                     /locus_tag="PLW15_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOT32942.1"
                     /translation="MPAAGRERVAADAASRGIPIEIIERPKANSLEEAAAILGVDPSV
                     IVKSLVVKRHDGTFLFALVPGDRQISWAKLRAVVGVNKLQLPDAALALEATGYERGTI
                     TPFGSTTAWPVYADERMLGARIAMGAGEHGYSAWVLADDLVTGFDATVADISD"
     gene            14824..15258
                     /locus_tag="PLW15_00675"
     CDS             14824..15258
                     /locus_tag="PLW15_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32943.1"
                     /translation="MPIDESHPNVVRFDRKLWVSTLPAEELAAQITAQRAWDDANSRV
                     NRWPWAIGVGAVIGTAVLYAATTLLGAPPVVNLFLLPVGFAVGAVLGGLVSERLRPRR
                     DADAALGDRPEVDPMTRIPRRVAARADADSTAQELIDLSTGR"
     assembly_gap    15482..15581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15582..16280
                     /locus_tag="PLW15_00680"
     CDS             <15582..16280
                     /locus_tag="PLW15_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32944.1"
                     /translation="YVPMDSTYVPFAHVVAAPTRIAPTRSANTLPIWLLAVSPMLSVV
                     AQAIVLAAPTTALIPILWALNLGLALGFIGLVIWDRIGIRGLGLTPSSPWWFIWWALL
                     PSPLVYLIVRRVSLKRQGVISNAPSNVFVLVLVASGLVAYFALLPLINARSASIAVDA
                     LETQLASALAHETATTWNVECPPAAPILTANSSFTCSADDSDGRAINLLITVDQRLNV
                     TFAPVDTAANTLSS"
     gene            complement(16277..16444)
                     /locus_tag="PLW15_00685"
     CDS             complement(16277..16444)
                     /locus_tag="PLW15_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32945.1"
                     /translation="MTDAMTAPLPVIVTPGLTLTPLGDPTAATCEGDFCAIPDHHTQA
                     IINRKLDEDAV"
     gene            complement(16441..17094)
                     /locus_tag="PLW15_00690"
     CDS             complement(16441..17094)
                     /locus_tag="PLW15_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOT32946.1"
                     /translation="MTEPIKVDIWSDVQCPWCYIGKRKFESGAAQFGGDVEVEYHSFE
                     LAPDTPVDFDGTPVDYLAQRKGLPTAQVEQMIERVVGIAESVGLDYDYDHIHQTNTVI
                     SHELLHFAKSKGRQLEMKERLLKAYFINGEHVGRIPDLVEIAVELGFDRDEVTEALES
                     HRFLPDVKADVALAREYGINGVPFFVIDGKYGVSGAQDAATFANVLSQVQAERETTP"
     gene            complement(17087..18115)
                     /locus_tag="PLW15_00695"
     CDS             complement(17087..18115)
                     /locus_tag="PLW15_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32947.1"
                     /translation="MTFRTRLVATLFACLAGSALVAAPGILAAPASGATTGAGIANIT
                     PYGGYLGNYIAPDGTRVYCIDPVLDWPSGITGDGVLTSSLTTSWGETLDADVLRKFDT
                     VLGRYGQTDDPVLAAAVSAYLYGFTSGYARTYGGGVDAALHFINGEPEVEATFLGIWE
                     EVEASSAPVPPSAEVTIEMSDADAGVVRISAVPADAAGQLILEGAVVADSGESTVAVS
                     GTDSVAIVSTPEDAAVDYSIAATATFVTAAGPQPQVTLYETGSQQRTIRDSGAAPVEF
                     ASSARVEVTRPVPTAETPAPTLAETGLAPSGVLGAGVALVTAGLIVPFARRSREYGSR
                     ARVIPERD"
     gene            complement(18209..19594)
                     /locus_tag="PLW15_00700"
     CDS             complement(18209..19594)
                     /locus_tag="PLW15_00700"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT32948.1"
                     /translation="MATPSNLDAVINLAKRRGFVFQSGEIYGGSRSAWDYGPLGTALK
                     ENIKKQWWQTVVQGRDDVVGIDSAVILPRKVWEASGHVEVFSDPLVESLHTHKRYRAD
                     HLLEAYEEKHGHPPVNGLADIKDPDTGQPGAWTEPQNFSGLLKTYLGPVDNEEGLHYL
                     RPETAQGIFVNFANVLGASRMKPPFGIGQVGKSFRNEITPGNFIFRTREFEQMEMEFF
                     VEPGTDEEWHQYWIDESLRWYTDLGINPENLRLYEHPKEKLSHYSKRTVDIEYRFRFQ
                     GSEWGELMGVANRTDFDLKTHSEASGTDLSYFDQTKDERWVPYVIEPAFGLTRALMAF
                     LIDAYAEDEAPNAKGGVDKRTVLRLDRRLAPVKVAVLPLSRNEQLSPLAKQVAADLRK
                     LWNVDFDDAGAIGRRYRRQDEIGTPFCVTVDFDSLDDNAVTVRERDSMEQERIPLERL
                     RGYLAEQLIGA"
     gene            complement(19699..20745)
                     /locus_tag="PLW15_00705"
     CDS             complement(19699..20745)
                     /locus_tag="PLW15_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT32949.1"
                     /translation="MTSSALTKAIDEFGDPRGYLAVASIGLPPRAAVDALSADLAAWA
                     AADRDPQGYDASIERARESYASIVGVPASRVAQGSQTSVLTALIAAAVPAGAEVLIPD
                     GDFSSIEFPFLQRTDIRVRSVPLAALADSITDDTWLVVFSLVQSANGIVADVDAIIES
                     ASRHHTYTFCDVTQAAGVLPVDASVFDVTVCHTYKWLCSPRGVAFLTLSERFDPHVTP
                     LQAGWYAGDDVWRSTYGPDMHLAHDVRRFDVSPAWQAWVGAEHSLALFAGLDIAEVWD
                     QASGLGDALCDTLGIPQQHQAIVTWPDESGTDLKKLVASGVRASGRAGRLRASFHLWN
                     DISDVDAVVAALRS"
     gene            20829..21770
                     /locus_tag="PLW15_00710"
     CDS             20829..21770
                     /locus_tag="PLW15_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT32950.1"
                     /translation="MDANLDLHSIRIVRAIAEHGTISAAARALGYSQPAISQHLRRSE
                     ARLGTPLVIRSGRGVRLSEAGQVVARHAVAITSALDAASGDLAELVGLAAGTVRVAAF
                     PTASSTLVPRLLASMRQRHPGITVGYVEAEPPEAIAMLRDGAVDLAITFAYPGDRSDP
                     HRPADVSLESVPLFTEPVVLALPDRHALADRQQVALADLADERWIAGCPLCRGHLLAA
                     CEAVGVTPNIELETDNAIAVLTLVAAGLGVALLPQLALATAPVPQGATVRSTTPSSDR
                     SIQLVVQNGSRRVPSIAATIELLEQVDGADWGLARVP"
     gene            complement(21767..22822)
                     /locus_tag="PLW15_00715"
     CDS             complement(21767..22822)
                     /locus_tag="PLW15_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT32951.1"
                     /translation="MPTTTTPIRTGIIGFGLAGRVFHAPFIATNPAFRLDLISTANPD
                     RAAEAAAQHPGAEIVATPDELLARAGDLDLVILASPAHTHLEQGLAALEAGAAVVIDK
                     PFVPTVSDAKKLIAAAEDADRVLTVFQNRRWDGDFLTVKRLIAEGALGTVHRFESTFE
                     RWSGPNRDRWQDTTTIEQGAGITYDLGSHLVDQALQLFGPAVVEQAELQIVRDGAVSD
                     DDAFISLLHSSGVRSHLTMSRAAAQSGPRFRVLGSESGYTVYGLDGQEPALKLQKWPG
                     SDGYGVTPESEWGLLGIDGSEAGLTPVPTEPGDYPAFYAGVAAAIRDGAPAPVDPRDA
                     LEVVRILERAHSLSAAD"
     gene            complement(22840..>23416)
                     /gene="uppS"
                     /locus_tag="PLW15_00720"
     CDS             complement(22840..>23416)
                     /gene="uppS"
                     /locus_tag="PLW15_00720"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOT32952.1"
                     /translation="VKHVSVYAFSTENWSRSPDEVRFLMGFNRDVLHRRRDQLNEWGV
                     RVRWAGRRPRLWASVIKELQFAEKLTAHNSVLTLTMCVNYGGRNELTDAVREIAADVA
                     AGRLSPTGISEKTIQRHLYQPDLPDVDLFVRSSGEQRTSNFMLWQSAYAEMVFLDTLW
                     PDFTRENLWHAIQLYASRSRRFGGAVDAPSV"
BASE COUNT         3858 a         7669 c         7960 g         3729 t
ORIGIN      
        1 agtccggcag ccaccgagag ggcgccgaat gagatcccat acgctgcgac ggcgaggccc
       61 accgcgaagc ttgcgcggat cgccgcagtg gtctccggat ctctggccaa acgacgaatc
      121 tccctccggg cacgtgggcc cagagggaga ctctatccgt tcagcggtcg gtgactaccg
      181 ggccactgga gggttgctgg cccgcagcac ctcgaggcga gcccggtact ccttctcgtc
      241 gatgtcgccc tgcgcgaagc gctcggcgag ggtcgactcg gcactgcgtg cgccggaacc
      301 ccagccgccg tggccgtgac cgaaggggcc gtagccgccc gcgcgcatcc agcgacgacg
      361 cccgatgctg gcgaacagaa cgaacagccc gaagaagaac aacgggatca ggaacaggag
      421 taggaagccg aagccgaagc ccgggccacc gaggtgagac gcgagagttg cgacggtgag
      481 agctgtaaac acgatgcatc cattcactag agggcccggt agtgggccac tgcgtcaaga
      541 ctgctcggcc tcgtcttctg gcgaatctgc cccgggaagt gttcttcact actcccccgg
      601 gtgcacaccg gtgccgcgga ctactccgtg cggccggggc tgaccagtcc ggtctcatac
      661 gcgatgacca cgagctgcac ccggtccctg gccgcgagct tggccatgat gcgggagacg
      721 tgcgtcttcg cggtgagcgg gctgaggaag agctgcttgc cgatctcctc attggtgagc
      781 ccctgcccga ccagggcgag cacctcgcgc tcgcgatcgg tgatgaccga caactggctc
      841 gcgtcgggcg tttcccgcag ttcgctcgac agacgttcga gaaggcgccg ggtcacggtg
      901 gggctgagca gcgcgtcgcc ggctgcgacg acgtgcacgg cgcggatgaa ctcgtcgggt
      961 tcggtgtcct tcacgaggaa tccgctggcg ccggcccgga tggcgcgtcc cacgtactcg
     1021 tcgagttcaa aggtcgtgac gatcacgatg tgggtctgcg ccaggtcggg gttgccgacg
     1081 atctgctcgg tggcccagag accgtcgccg tcgggcatcc ggatgtccat gagcatgaca
     1141 tcgggacgcg tcgcgaccgc gagcgatacg gcctccgagc cgctgccggc ctcccccacg
     1201 atctcgatgt cgggctcggc ctcgagcagg ctgcggaatc ccgcgcgaat cagctgctgg
     1261 tcgtcggcga ttgcgacgcg gatcatgcgt gctccttctg gggtatggcg accatcacga
     1321 gcaggccacc ccgcgggccg ggaccgacct cgagcgtgcc accgagcagc tctgcgcgct
     1381 cccgcatgcc gagcagcccg cggccggtcg tgatgtcacg cccgtcggag agccccaccc
     1441 cgttgtcggc cacggcgatc aggtagctgc cgtcggcctc cgagatgtcc acggtcgccc
     1501 gcgtggcgcg ggcgtggcgc acgacgttgg tgagggcctc ctgcacgatg cgatagatcg
     1561 ccagctgcac ggcctgcggc gcggcatcca gcgagttgga gagggtcacc tccaccccct
     1621 gagacgtgac ggatgccgcc agccccgtca gcctcgagag gtcgggttcc ggcaccagcg
     1681 gtgccgtcgg atcccggccg tcctcggccc gcaggacccc gagtaccgac cgcacctcgt
     1741 cgagagcggt cttactggtc tccttgatgc tcgcgagcgc ctcggaggcc ttctcgggct
     1801 gcttgtccat cagatggaga ccgacgctgg cctgcacgtt gatctggctg agcgaatggg
     1861 cgagcacgtc gtgaagctcg cgcgcgatgc gcacccgctc ggcctgcacc tcggactgct
     1921 tgcgctgggt gtactcgcgt cggtactcct cgtagtgctc ccggcgggtg cgcatgccct
     1981 ccgcgagacc gaagacgatc aggatgccca gagtcgtgat ggcgatctgt gccggacgcc
     2041 aatcgaggcc gaccatgaaa ccgaccacca gggtgaccag ccagccggtg ccgacggaga
     2101 tccacgccca gagccgagcg ccgcgcagca cggcaccgcc gatcgcgaac gcgagcgcga
     2161 tgaacggcgg acccggttgg gagatggaga gcacaagatc gacgcaggac gcggcggtca
     2221 ccagcgcgac gacgggaccc ggaaagcggc gtgcggcgag gagcaacagc ggcccgatga
     2281 ccgacagcgc ggcggcgatc agcaccgacg gcgaatgctg cctctgaaac aggatccagt
     2341 agagcgaggg cggcacctgg atcgcgagcg agacgaagac gggaagcaga agtcgcattc
     2401 gtgccgacga ccgaggacgc ggcatatcca aaccgtcggg ccagcgcccc tgccagccgt
     2461 gctgccagcg atcggggtcc cagcgggaac gccactgacc tcgccgggga tctcgcggct
     2521 ggtgcatccc tcgatcgtag gcccgtgcgc gccccgcggc atcggccggg cggtgtcgct
     2581 cacgtactcc cccgggagta ggtgagtctg ccaacaggaa ctgcgcgcca acaggaactg
     2641 cgcgccaaca agaaaggccc gggtcgtgga ggacccgggc cttcgctccc cgacttggac
     2701 tcgaaccaag aacctatcgg ttaacagccg attgctctgc caattgagct atcgaggatt
     2761 ggcttttcag cttgcctact ttaccaaagg atgcgcgatc cccaaaccga gttcgacact
     2821 gcggcgtcaa tagccggccg cggggtcgac aacgcccacg aagcgaccat cgccgagcag
     2881 cgcctcgacg ttgcgtcgca cccgttcggc gagcagcggc tccgtcatct cgggagtatc
     2941 ggcggtgtga ccggtgacga tgcagcgggg tgcctgccag agcggatgcc cgtccggaag
     3001 cggttcgggg tcggtgacat cgatgccggc ccccgccagg tggcccgagt tgaggctcgc
     3061 cagcagagcc tcctggtcga ccagagcgcc gcgggcgatg ttgatgagtg cagcgttggc
     3121 cttcatcgcg gcgagctcgg tcgcaccgat gagctgggcg gtctcgtcgg tcgccgcggc
     3181 cgcaagaatg acgacatcgg cgtcgggcag caccgacaac aggtcggcga cgggaaccgt
     3241 gcgctgcgca cctggcagcg gatcggccga acggcgcacg atcgtgaact cgacctcgaa
     3301 cggggcgaac agacgcatga cctcgaccgc aatgcccccg gcacccacca ccagcacgtg
     3361 gcgaccgtag agggatgttc cgaccttcac cggcgcccaa ctggtggcac gagccttctc
     3421 ggggagcccg cgcagcacgg ccagagtcag tgccagggcg tgctccgcca cgggctcgga
     3481 gtacgagccc ttggcactgg tccacagcgg gctgctgcta ccggcgaagc gcttgagtgt
     3541 cgacgcgaac gcatcgacgc cggcccaggg cagctgcacc cagccgatcc ccggatgcga
     3601 ctgcaggaga tcgacgagct cgggcgtttc agccggcgcg agccagatca ggcctcgcgt
     3661 ctcgtcggag agcggcgcca cgacaccgcc cgctgactcc accgcggcga cgaaggacgc
     3721 gcgcggttcg gggaggatgg cgatcggacc gggctggggg cgcgtgagtg gggcggcgga
     3781 cggcgcgacc aggtcgcgat gctcgccggg ggtgcgacgc gtcatccggc gtcctgcaga
     3841 tggtcgagcg atcgggccag gcctgcgaca tagggatgac gcggatcggc cagcacctct
     3901 tcgagctgcc cgagccccac gaagattccg ccgtgcatga tcgcgacctg ggtgctcacc
     3961 cgccgcacct cggcgagatc ggccgtcacg atgagggccg agaacgcgcg ttgcttctgc
     4021 aggtgcacga ggatgtcgag cacggcgctg cgcaccgtca catcgattcc cgccgtgggg
     4081 tcatcggcga tcagcagtgt cggctccagg atgagggcct tcgcgatcgc cacccgctgc
     4141 cgctgaccct tgctgagctc gtgggggtac ttgttcatgg tgcccagcgg aaggcgcacc
     4201 gcgtcgatga gggtcgcgac ggcgtatccc gcctcgtcct gatcgaagcg gcggtcacgc
     4261 gagaagatcg gctccgcgac gttctcgccg accgtcaggc gtggctcaag gaagtcgccg
     4321 gcttcctgcc gcaggtagcc gacctgcatc tgaagccgat cccgcttgcg ggcggagatg
     4381 ccacgcaccg gcgtgccgag cacggtcaac gcgccaccgc tgatgaccgg cgagtcggcg
     4441 gccgagaggt cggcctccag agcgatcgct cgcgcgagcg tgctcttgcc cgaaccggtc
     4501 tcccccacga ccgcgagcac ttcgcccggt tcgatggtga agctcacacc gttgacggcg
     4561 accgaccgcg tgtccgggtt ctccgagggg tagcgcagtg acaggtcctg cacgacaacc
     4621 gcgctcattg aactccccgg gtcagtgcgc gaagttcctc acggcgaacc gcctgctcgg
     4681 ccggatccgg cacgggcagc gacgccagaa gtcgctgggt gtacggatgc tgcggcgctc
     4741 ccagcacctg cgagggaggc ccctcctcca cgagctcgcc gcggtacagc acggcgatgc
     4801 gatcggagac cgtgtcgacg acagcgaggt cgtgactgat gaagagggcg gcgaatccga
     4861 attggttctg cagctcggcg aacagttcga gcacgcgggc ctgaaccgac acgtcgagcg
     4921 cgctcgtggg ctcgtcggcg atgagcaggg tgggttcgag tgcgagcgcg cgtgcgaggc
     4981 tggctcgctg gcgctgtcct ccgctcaact cgtgggggta gcggtcgccg aaagcccgag
     5041 gcagctgcac cgcttcgagc aactcgtcga cgcgggggcg tgctgccgtg gcatcccccg
     5101 cccggccgtg cacgatgagc ggctcggcaa cgcactgggc gatcgtcagc agcgggttga
     5161 agctcgatgc aggatcctgg aagacgaagc cgatctgctc gcgcaccggc ttgaagcccc
     5221 gctcccgcac tgtgagcatc tcgtggccga gcacctgcag cgatcccccg ctgatcttgg
     5281 tgagaccgcc gatggcacgc ccgatcgtgg tcttgccact gccgctctcg ccgacgagtc
     5341 ccagcacctc gcccggggcg atggtgaagc tcacgcggtc gacggcccgg aatccacccc
     5401 gaccgaagcg accggggtac tcgatcacga ggtcggtggc ctgcaccacc ggcggagctt
     5461 ccgccactgg cgtgcgctca cgcgtcgtgt cgatgcgagg caccgccgca agaagcttct
     5521 gggtgtacgg atgctgcggc gagccgaaca acttcgtcgc ggtcgcctgc tccacgacct
     5581 tgccctcgta catgacgacg acacggtcgg cgagatcggc gacgacaccc atgttgtggg
     5641 tgatcaggat gatcgcggta ccgaactcgt cgcggcaccg gcgcagcagg tcgagaatct
     5701 ccgcctgcac cgtcacgtcg agcgccgtgg tgggctcgtc ggcgatgatc agttcgggat
     5761 cgagcgcgag cgccatagcg atcaccacgc gctgcttctg gccacccgag aactggtgcg
     5821 ggtagtggtc gatgcgcttc tctggttcgg gaatgcccac cttgcccagg tactcgatcg
     5881 ccttggctcg cgcctggcga cggctgtact tgccgtgggc gcgcagaccc tcggcgagtt
     5941 gccacccgac cgtgtagacg gggttcagcg aggtcgacgg ctcctggaag accattgccg
     6001 catccagccc acgggtggcg cgaagcttct cccccgtaag tcccacgacg tcacgaccgt
     6061 tgagcagaat cacgccacga gtgactgcgg tctcgggcag caggccgatg atcgacttgg
     6121 ctgtcaccga cttgccactg ccgctctcgc cgacgatcgc gacgacctcg cccgagtcga
     6181 ccgtgagact cacgtcatcg accgcgggga tgtcaccccc gtcggtcgcg aaggtgactt
     6241 cgagattgcg gatgtcgacg accgtcttca cgactgcacc tccagcggat cgggggtgtg
     6301 cgccgcgacc tccgcacgcc ggcgcgtgcg caggcgagga tccgcgaggt cgttgaggct
     6361 ctcgcccacg agcgtgatgc cgagaaccga gagcacgatc gcgagcccgg ggaacagcga
     6421 ggtccaccag atgccgctcg tgacgtcgga cagcgacttg ttgaggtcgt agccccactc
     6481 ggcggccgcg gtcggctcga tgccgaagcc gaggaagccc aggcccgcga gcgtgaggat
     6541 cgcctccgag ctgttgagcg tgaagatcag cggcagcgtg cgcgtcgcgt tgcgcagcac
     6601 gtgctgcacc atgatgcgcg gcgcactcga gccaatgacc ttcgccgact cgacgtaggc
     6661 ctccgacttg atacgcacgg tctcggcccg gatcacccgg aagtactgcg gaatgaacac
     6721 gaacgtgatc gagatcgcgg ccgcgaggat gcctccccag aagctcgact gaccgcccga
     6781 gatcgcaatg gcggcgacga tagccaagag cagcgacggg aaggcgtaga tcgcgtcggc
     6841 gatgaccacg agcacgcggt cgagccaccc gccgatgtat cccgagatga gcccgagggc
     6901 gactccggcg aagatcgaca gcagcacggc cacgatgatc acgatgactg cggtctgggt
     6961 tccccagatc actcgggaga gcacgtcgta gccgccgacg gtggtgccca gcaggtgcgc
     7021 gccgccggga ggaagctgcg ccccgaatgt tccgctcgcg tcgcgcagct gcgaatagcc
     7081 gtacggggcg aggagggggg cgaagatggc ggtcagcacg aatacggccg tgatggcaag
     7141 gcccgtgacg agcatgccgc gctggagccc cacgctctgg cgcacctgac gcaccaccgg
     7201 caaccggtcg agaatcttgg tcatcagtac ctcacccgcg ggtcgatcag cgccgcaatg
     7261 atgtcgacga tgaagttggt gagcgccacg atcacggcca gaagagcaac gatgccctga
     7321 accgcgacga agtcacgggc ggtgaggtac tgggtgagca tgaatccgag cccctgccac
     7381 tcgaacgtcg tctctgtgag caccgcacca ccgagcagta cggcgatctg cagtccgatc
     7441 acggtgatga tggggatcga cgcgggcttg agcgcgtgct tggtgacgag ccggtactcg
     7501 cgcaccccgc gtgatcgcgc ggagtcgacg tagtccatcg acagcgtgcc gatcacgttg
     7561 gtgcgcacca gacgcaggaa gatgccggcc gtgagcagac ccagcgccac accgggcaac
     7621 acggcgtggg ccaacagatc ggcgtagacc gcgcccgggt tcgacgtctt cagcgcgtcg
     7681 atcaggtaga tgcccgtggg gttgtcgacg cgggtcagtt gtagctccgt gcgaacggat
     7741 gcccgccccg cgacgggaag ccagcccagc cccaccgcga agatgagctt cagcaacagg
     7801 ccggcgaaga acacgggggt ggcatagcag aggatcgcga agacccgcag gatcgcatcc
     7861 ggccaccgat cgcgcacgta ggccgcgagc atgccgagcg ggattcccac gatgaacgcc
     7921 acgatcagcg cgtagaaggc gagctcgagc gtcgcgccgc cgtaacggac gagcacgtcg
     7981 accaccggac ggtgatcgct gatcgtctcg ccgaagttgc cggtgaacac ctgaccgatg
     8041 tactcgaagt actgcaccag gatcgggcgg ttgtagccgg cctcctcgat gcgctgcgcc
     8101 agttgatcgg cgggaaggcg cccaccgagt gcggcggtga tcgggtcacc gatcgtgcgc
     8161 atgagcacga agaccaaggt gaccaggatg aagatcgtgg ggaagatgag caggaaccgc
     8221 acgatgatga accgcagcag cttgttgccg ccagtgccgg gtcgcgtcgt cggtacgacc
     8281 gacgcaggtc cggcgatctc gggtgccgtc atcgccgcct catgtcttcc taaatgtcgt
     8341 gcacgagggg cggcccgcgg acggaccgcc cctcgctact agctgggatc gactctagcc
     8401 gctgagcgac tagcccttca cgagggtggc gaaccggaac ttgaacgacg cgtcgagcgt
     8461 gagcccgtcg atcgtcttgc ccgagaccgc gacctgtgca ccctggagca ggggcacggt
     8521 cgacaggtcc tcaccgacga ccgcctggat ctgctcgatg atcgccgtac gctcggccgg
     8581 gtcctgggtc acaccctgct gggtgatcag gtcgtcgacc gtcgggttcg agtagtggtt
     8641 gctcaggaag ttgttcgtca ggaagaacgg cgtcaggtag ttatcggcgt ccgagtagtc
     8701 cgggaaccag ccgagctggt acgcggggta gacgtcgttg acgcggtcct cggagtactg
     8761 aacccactcg gtggactgca ggtcgaccgt gaacaggcca tcggcctcga gctggctctt
     8821 caccagcgcg tactcgtcac ccgaggaggg tccgtagtgg tccgggttgt actgcaggct
     8881 gagcgcgacc ggcgtcgtga caccggcggc ggcgaggatc gcggccgcct tcgtggcatc
     8941 cgggccgccc gagccatcac cgtagaggtc cttcagcacg gtggtggcac cggtgaagcc
     9001 gtccggaacg aaggagtaca gcggggtgta cgtgcccttg tagacctggt cggcgatctc
     9061 ctggcggtcg accagcgagg cgacagcctg gcgaacggcc tgcgccttgg ccgggtcggc
     9121 ctcagcggtg gtcgcgccgt acggctgcgt gttgaagttg aacacgatgt agcggatctc
     9181 gccaccgggg ccgtcgtgca ccgtgacgtt gtcgtcggca cggaggtcgg cgacgtccgt
     9241 agccgagagg ctgcggtacg cgacatcgat gttgccctgc tgcacgtcga gcttgaggtt
     9301 cgacgagtcg gcgtagtagc tcaggatgac cgagctgttc tgtgccgggc cgagcagacc
     9361 tgcgtagttc gggttcggaa cgaactcgat cgtcttgttg aagtcgtagc tggcgatctc
     9421 gtactggccg ttgaaggcct tgcccgcgac gatgtcgtca tccgaggtca gctcggtggc
     9481 cgagaacacc tcttcgtcga cgacggcgcc ggggaaggtg gtgaggatct gcgggaacac
     9541 ctggtcgttg gccgccttca ggtggaagac gacggtcgtg tcatccacag cctcggtgga
     9601 gtcgaggttg tagagcaggc tcgaagggcc atcgggtgcg gcgatcgcga gctggcggtc
     9661 gaacgtgaac ttcacgtccg atgcggtcag gtcgtggccg ttcgcgaact tcagatcggg
     9721 cttgagcacg accgtgtagt cgttcggagc cgtgaactcg gcggactccg cgaggtcggg
     9781 ctgcgggtcg gggctgccca gcggcgagtt gaggaggaag gcgtaaatct ggttctgcac
     9841 ggcgaacgag ccgttgtcat acgaaccggc cgggtcgagc gtgacgacct tgtcggtggt
     9901 gccgaccagg atcgcaccgt cgtcggtgcc accgcctgat gcggtgcagc cggtcagcgc
     9961 gagcgcgaat gctgcggtgc cggcgagcac gccgagggcg cgcttcgtgg atgcggatgt
    10021 catatccgtc tacctcttcc tgttgtggag acaccttcgt ctcacccccg aatggggggt
    10081 aggactaggt tgctgtggat tcaggtctaa cacacggaac cgggcggtcc cgcatccaag
    10141 accattgatc tgacgattct ttacgggaat gaaacgtcgt cggccgcaaa cgcaagaatc
    10201 acgcgctcga atcaattatt cgtcgcgcaa tgctcgccgt tccgcctcga cgcgcacgag
    10261 gtcgcgctgc acctgcgcgt agcgctccgg ctccgcaacc gagtcgatgc gctgcatggt
    10321 tccgagaagg tcgcgcttct gacgcagcag atcgcggtcg accagagccg cggacacgcc
    10381 cctgcagtac acgccgatga gctcggcccg ttcgggaatc ggtgcgacgc cgagctgctt
    10441 gacgagggtc tcgaacggag cgggcacctc ggccgcgacg cgcgagagcc agtcggggga
    10501 cgcgatgtgc tcgatgctcg tggcgatcgc gtcacgcacc acagacagcg tgttgtcgac
    10561 gaagtcgacc gacgacgccc gccgcacgag gtccgcgccg acctcgctcg ggtactgcag
    10621 catcgccatg agagcgtcac gctcgagcct cgtcgcaggg tctgtgggga gcttcctgag
    10681 atcgagggcc tcggcagcgg gctgctcctg caccgacgac cgatcgggct cggacgcgcg
    10741 cgccgacgca ccccgcacgg atgccacagc gcgccccacc tcgccgggat ccatgccgag
    10801 ccaccccgcc aggttgcgca cgtaccccac gctcagtgcc tggtcccgga tgctcgcgac
    10861 aaccggggcc gcaccgcgca gtgcggccac cctgccctcg acggtctcga ggtcgtgcgc
    10921 agccagctcg cgtcggatct tgaactcgaa cagcggccgt cgggtcttca ccagccggcg
    10981 caccgcgtcg tcgccgcggt tgagccgcag gtcgcagggg tccagaccat cgggcgcgac
    11041 agctacgaag gtctgcgcgg caaagcggtg ctcctcggcg aatgcgcggc tggccgcctt
    11101 ctggccggcc tcgtcagggt cgaaggtgaa tatcacctcg ccggtgccgg tcgtgtccgc
    11161 gttatcgaca tcgccgatca tgcgacgaac gagcttgatg tggtcgaccc cgaatgcggt
    11221 gccacaggtg gcgaccgcgg tcgtgacgcc cgcgaggtga caggccatga catccgtgta
    11281 gccctcgacg accacgacct ggcggccacg cgcgatgtca cgcttggcca gatcgagccc
    11341 gtacagcacc tggctcttgt ggtaaaccac ggtctcgggg gtgttgaggt acttgggtcc
    11401 gccgtcgtcg tcgagcagct tgcgggcgcc gaagcccagg gttgcgccgg tggcatcccg
    11461 gatcggccag atcagccgtc cgcggaaacg gtcgtagacg tcgccgcgat cgccgcgccc
    11521 gagcagtccg gcggagatga gctcctcctc ggtgtagccg agggcggcga gtgagcgccc
    11581 gagggctccg ccccgaggag cgaacccgag cccgaagcgc tgcgccgctg aactgtcgaa
    11641 accgcgctcg ccgagaaacc gtcgcgcggg ctcggcctcg tcggtcgtga gctgcgcgac
    11701 atagaactcg gctgccgcct gattggccgc gaggatgcgc gcgcgcacgc cgtgatcggt
    11761 cgccgccccg ccgtcttcgt agtgcagttc atagccgatg cgcgccgcca tgcgctcgac
    11821 cgcttcggag aaggacacgt ggtccatctt ctgcaggaac gtgaagacgt cgccgccctc
    11881 accgcagccg aagcagtggt agaagcccac ctgcggtcgc acgtggaagc tcgggctgcg
    11941 ctcctcgtgg aacggacaca ggcccttcat cgacccgacg ccggcgctct tcaggctgac
    12001 atagtccccc acgatgtcgg cgaggttggt gcgggagcgc acctcgtcga tatcgcttcg
    12061 tttgatgagg ccagccacgt gttgattgta agtctggttt cgatacgccg ttccggctac
    12121 tcaaccgacc aggcgttcat gccaagccat cgccgactgg tcggtgaggg atgccacctg
    12181 gtctacgacc gcgcgcttgc gctgctggtc tgtcgccgcg gcatcccagt cagcacggaa
    12241 tccgacatcg aggtgggcgg gaccggtcca cagcagcaca tccgcgagtt cggagaggat
    12301 gtcgcgctgc tgcgcataga tcggccggcg agcgttggtc gacatgacga atgccgcgac
    12361 gatgcccttg agcacggcga tctcagcctg gatctcgcga ggcacaacaa cgtcggcgcc
    12421 gaaccgggac agcactccgg aatgagcggc ccgggttgcc tgagtggcgg caccggcgaa
    12481 acggccgatc agctgactgg tgaggttctt gaggcgaccg tgtgcctgac gcgagccatc
    12541 ccagcggtcg atccagaagt cgagactgtc gagccggtcg aaggccgcga tcagctcgtc
    12601 gtgggtgacc tccccgccga tccactcgaa catcgagttg acgaggtcgt cgtggtcgac
    12661 gcgggcaccg agggcggtga cgtccacgta cccattgact atcgcgtcct cgaagtcgtg
    12721 caccgagtag ccgatgtcgt cggagaggtc catgacctgt gcctcgatgc accgacggcg
    12781 atccggtgcc ccggcacgca accactggaa gacctcggtg tcgtcgtcgt agaagccgta
    12841 cttgctgcgc ccactcgggt cgggcacgcc ctgggccgcg ggccacggat acttgcaact
    12901 ggcgtcgagg ctggcccggg tgaggttgag cccgtagctg cggtcgtcgt cgccgaagac
    12961 cttcggctcg atgcgggtga gcacgcgcag ggtctgcgca tttccctcga aaccgccgat
    13021 gtcggcggac cagtcgttga gcgcgcgctc accattgtgg ccgaacggcg ggtggccgag
    13081 gtcatgggcg aggcacgctg tatcgacgac atcggggtcg agcccgagac tgtcggcgag
    13141 ctcgcggccg acctgcgcga cctccagcga gtgggtgagg cggttgcgcg cgaagtcgag
    13201 gccggccgtc gggctcagca cttgcgtctt ggccgccaac cggcgaagcg cgctggagtg
    13261 cagcacgcgg ccgcgatcgc gggcgaagtc gctgcggcga ctcgagtgct cctccggcag
    13321 ccagcgctcc cggtccgctg ccgagtattc cgagctatcc gccactggtg ctcaactcgg
    13381 cttcggtgag ctcggcgcgg tgggtctcct gaagctcgcg actgtcgagc cagccctgcg
    13441 gcaggctcgg gctcttcggg gttcccgcgc gaccgcgctg gccctcnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnaggc acccacggcc caccaccacg ccgtcgcagc
    13621 cggtctcgtc gaccatgcgc agagcgtcat ccgcgctcca gatgtcaccg ttaccgagaa
    13681 ttggcgtgtc ggtgatcgtg ttcttcagct tctcgatggc cgaccagtcg gcagtgcccg
    13741 agtagaactc ggcggcggtg cgtgcgtgca gagcgatact cgcgaccccg gcgccggacg
    13801 cagcgcgggc cgcctcgagg taggtgaggt ggtcgctgtc gatgcccttg cgcatcttga
    13861 cggtcagcgg gatgtccccc gccgccttca cggcgcgctc gacgatctcg cggaacaggt
    13921 cccgcttcca cggcagtgcc gctcccccgc ccttgcgcgt gaccttgggc accgggcatc
    13981 cgaagttcag gtcgatgtgg tctgcgcggt cttcggcgac cagcatcgtg actgcttcag
    14041 agacggtttt cgggtcgacg ccgtagagct ggatgctgcg ggtggtctcg gactcgtggt
    14101 gacggatcag ccgcatgctc tccggggtgc gctcgaccag ggcacgactg gtgatcatct
    14161 cggagacgta gaggccagca ccgtactcgc gacacaaacg acggaacgcg gtgttcgtga
    14221 tgcccgccat gggcgcgagc acgaccggaa catcgagcgc aatgggcccg atgtgcagcg
    14281 tgggtgctgc gctcgtctga gcgtccgcga gaatggtcac gtgacaattc tcccagacaa
    14341 tgacgggatg cccgccgcag gccgcgaacg ggtggccgcg gatgccgcga gccgtggcat
    14401 cccgatcgag atcatcgaac gcccgaaggc gaactcgctc gaggaggcgg ccgcgattct
    14461 gggcgtcgac cccagcgtga tcgtgaagtc gctcgtggtg aaacggcacg acggaacctt
    14521 cctgttcgcg ctcgtgcccg gcgaccggca gatctcctgg gcgaaactgc gcgccgttgt
    14581 gggagtcaac aagctgcagt tgcccgatgc cgcgctcgcc ctcgaggcga ccggttacga
    14641 gcgcggcacg atcaccccgt tcggcagcac gaccgcgtgg cccgtctacg ccgacgaacg
    14701 gatgctcggc gctcgcatcg cgatgggcgc gggtgagcac ggctacagtg cctgggtgct
    14761 cgccgatgac ctcgtcacag gattcgacgc caccgtcgcg gacatcagcg actaggacca
    14821 cacatgccca tcgatgagag ccaccccaat gtcgtgcgat tcgaccgcaa gctgtgggtg
    14881 agcacactgc ccgccgagga gttggcggcc cagatcaccg cgcagcgggc atgggatgac
    14941 gcgaactcgc gggtgaaccg ttggccctgg gcgattggtg tcggagcggt catcggcacg
    15001 gcggtgctgt acgccgcgac gacgctgctc ggtgccccgc ccgtcgtgaa cctgttcctg
    15061 ctgccggtcg gcttcgccgt gggtgccgtg ctcggcggtc tcgtcagtga gcgactgcgc
    15121 ccgcgacggg atgcggatgc ggcgctcggc gatcggccgg aggtggaccc gatgacgcgc
    15181 atcccgcgca gggttgccgc ccgggcggac gccgactcca cggctcagga gctcatcgac
    15241 ctgtcgacgg gtcgctgagg gcttaccctc cgtcgcccga acgggggatc gagctatcct
    15301 cacaggctgt ctagactgct gcgcggggga ggttgctcgt gtcagacccg tcgactgcgt
    15361 acagctcggc accagcggcc tatccgcctg ccgactggta tcccgatccc accggtgaag
    15421 gccaactgcg ctggtggggc ggcttggctt ggacagagca tgtcaagcct ttcccggcgc
    15481 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntacgttccc atggactcga
    15601 catacgttcc gttcgcccac gtcgtcgccg cgccgacccg cattgcgccg acccgttcgg
    15661 cgaacactct gcccatctgg ctgctcgccg tgtcaccgat gctctccgtc gtcgcccagg
    15721 cgattgttct cgcggctccc acgacggcgt tgattcccat cctgtgggcg ctgaacctcg
    15781 gcctcgcgct cggcttcatc gggctcgtga tctgggaccg gatcggcatc cgcggcctcg
    15841 gtcttacccc gtcgtcgccg tggtggttca tctggtgggc gctactaccg agccccctcg
    15901 tgtacttgat cgttcgccgc gtcagcctca aacgccaggg cgtgatctcc aacgcaccca
    15961 gcaacgtgtt cgttctggtg ctcgtcgcca gcggcctggt ggcctacttc gcccttctgc
    16021 cgctcatcaa tgcacgcagt gcgtccatcg ccgtggacgc actcgaaact cagctcgctt
    16081 cggcacttgc tcacgagaca gcaacgacct ggaacgtgga gtgcccgcca gccgcgccca
    16141 tactcactgc caacagcagc ttcacctgct ccgccgacga ctccgacgga cgcgcgatca
    16201 acttgctcat cacggtcgac cagcgactca atgtgacctt cgcacccgtc gatacggccg
    16261 cgaacactct ctcgagctag accgcgtcct cgtcgagctt gcggttgatg atcgcctgtg
    16321 tgtggtggtc agggatggca cagaagtcac cctcgcacgt ggcagccgtc gggtcgccta
    16381 gcggggtgag cgtcagcccg ggagtcacta tgacgggaag aggggcagtc atggcatccg
    16441 tcacggggtg gtttctcttt cggcttggac ctgtgagagc acgttagcga aggttgcggc
    16501 atcctgagca ccggacaccc cgtacttgcc gtcgatcacg aagaacggaa ccccgttgat
    16561 gccgtattcg cgggcgaggg cgacgtcggc cttcacgtcg ggcaggaagc ggtgcgattc
    16621 gagcgcctcg gtgacttcgt cgcggtcgaa gccgagttcg acggcgatct cgaccagatc
    16681 cgggatgcgg ccgacgtgct cgccgttgat gaagtaggcc ttgagaaggc gctccttcat
    16741 ctccagctgg cgacccttgc tcttggcgaa gtgcaggagc tcgtgtgaga tcacggtgtt
    16801 ggtctggtgg atgtggtcgt agtcgtagtc gagaccgacc gactcggcga tgcccaccac
    16861 ccgctcgatc atctgctcga cctgggcggt cggcagaccc ttgcgctgag ccaagtagtc
    16921 gaccggggtg ccgtcgaagt cgacaggagt gtcgggagcc aactcgaacg agtggtactc
    16981 gacctccacg tcgccgccga attgggcggc gccggactcg aacttgcgct ttccgatgta
    17041 gcaccagggg cactgcacgt cggaccagat gtctaccttg atgggttcag tcacgttcag
    17101 ggataacgcg cgctcgcgat ccgtattccc gggatcggcg tgcgaacgga acgatcagtc
    17161 cggcggtcac gagcgcgact ccggcaccga gcactccgga gggcgcgaga ccggtctccg
    17221 cgagcgtcgg cgctggtgtc tcggccgtgg gcaccgggcg ggtaacctcc acacgggccg
    17281 acgacgcgaa ctccaccggc gcggcgcccg aatcccggat ggttcgctgc tgggagcccg
    17341 tctcgtacag agtcacctgc ggctgagggc ccgccgcggt cacgaacgtc gcggtcgcgg
    17401 cgatcgagta gtcgaccgcg gcatcctcgg gagtgctcac gatcgccacc gaatcggtgc
    17461 cgctgacggc aactgtcgac tcccccgagt cggctacgac cgcgccctcg agaatcaact
    17521 gccccgcggc gtccgcggga accgccgaga tgcgcacgac gcccgcatcg gcatcgctca
    17581 tctcgatggt cacctcggca gacggcggca ccggcgcgct cgacgcctcg acctcctccc
    17641 agatcccgag gaatgtcgcc tccacctcgg gctcgccatt gatgaagtgc agcgcagcgt
    17701 caacgccacc gccgtaggtg cgcgcgtacc cgctcgtgaa accgtagagg taggcactca
    17761 ccgcggcggc gagcaccggg tcgtcggtct ggccgtaccg gcccagcacg gtatcgaact
    17821 tgcgcagcac atcggcgtcg agcgtctcac cccaactcgt cgtcagactc gacgtgagca
    17881 cgccatcacc ggtgattcca ctcggccagt cgaggaccgg gtcgatgcag tacacgcgag
    17941 tgccgtcggg cgcgatgtag ttgccgagat agccgccgta gggggtgatg ttggcgatac
    18001 cggcgccggt ggtggccccg gaggcgggcg ccgcgaggat gccgggtgcc gcgacgaggg
    18061 cggagccggc gaggcaggcg aacaaggtgg cgacgagtcg ggttcggaag gtcatgccag
    18121 aagggtctga catgagccct gcggcacggg ccgcgatctc ctagcctgtg gaatacccgc
    18181 gccgcggcat ccctgtggac aatcgcgttt aggcgccgat cagctgctcg gccaggtaac
    18241 cgcgcagacg ctcgagcggg atgcgctcct gctccatgct gtcgcgctcg cgcacagtca
    18301 cggcgttgtc gtcgagcgaa tcgaagtcga ccgtcacgca gaacggcgtg ccgatctcgt
    18361 cctgacgacg gtagcgacga ccgattgccc ctgcatcgtc gaagtcgacg ttccacagct
    18421 tgcgcaggtc ggcagcaacc tgcttggcca gcggagagag ctgctcgttg cgggacaacg
    18481 gaagaacggc gaccttgacc ggcgcgagtc ggcgatcgag ccgcagcacc gtgcgcttgt
    18541 cgaccccgcc cttggcgttg ggcgcctcgt cttcggcgta ggcgtcgatg aggaaggcca
    18601 tgagcgcgcg ggtgagcccg aacgcgggct cgatgacgta cggcacccag cgctcgtcct
    18661 tggtctgatc gaagtaggag aggtcggtgc ccgatgcctc cgagtgcgtc ttcaggtcga
    18721 agtcggtgcg gttggcgacg cccatgagct cgccccactc gctgccctgg aagcggaagc
    18781 gatactcgat gtcgacggtg cgcttcgagt agtggctgag cttctccttg gggtgctcat
    18841 agagccgcag gttctcgggg ttgatgccga ggtcggtgta ccagcgcagc gactcgtcga
    18901 tccagtactg gtgccactct tcgtcagtac cgggctcgac gaagaactcc atctccatct
    18961 gctcgaactc gcgcgtgcgg aagatgaagt tgccgggcgt gatctcgttg cggaagctct
    19021 tgcccacctg accgatgccg aacggaggct tcatgcggct cgccccgagc acgttggcga
    19081 agttcacgaa gatgccctga gcggtctcgg gccgcaggta gtgcagtccc tcctcgttgt
    19141 cgacagggcc gaggtaggtc ttcagcaggc cggagaagtt ctgcggctcc gtccaggcgc
    19201 cgggctggcc ggtgtcgggg tccttgatgt cggcgaggcc gttgaccggc ggatgcccgt
    19261 gcttctcctc gtacgcctcg agcaggtgat cggcgcgata gcgcttgtgg gtgtgcagcg
    19321 actcgacgag cgggtcggag aacacctcga cgtgccctga cgcctcccac accttgcgcg
    19381 gaaggatcac cgcggagtcg atgccgacca cgtcgtcacg accctgaacg acggtctgcc
    19441 accactgctt cttgatgttc tccttgagcg cggtgccgag cggaccgtag tcccacgcgg
    19501 agcgcgaacc accgtagatc tccccggact ggaagacgaa accccggcgc ttggcgaggt
    19561 tgatgacggc gtcgagattg ctgggggttg ccacgagaac tccaataaac agccgagctg
    19621 gatgcccggt cggcggttgc gccagtctaa cgaagctcgc cggggccggg gaccacgcgg
    19681 gggtggctag gagtgcggct aggagcgcag cgctgccacg accgcatcga catccgagat
    19741 gtcgttccac aggtggaacg aggcgcggag gcgtccggcg cgacccgaag cgcgcacgcc
    19801 cgacgcgacg agcttcttca agtccgtgcc ggactcatcg ggccaggtca cgatcgcctg
    19861 gtgctgctgc gggatgccga gggtgtcaca cagggcgtcg ccgagcccgg aggcctgatc
    19921 ccagacctcc gcgatgtcga ggccggcgaa cagagcgagg gagtgctcgg cgcccaccca
    19981 cgcctgccag gccggagaca cgtcgaaccg acgcacatcg tgggccagat gcatgtccgg
    20041 accgtaggtg ctgcgccaca cgtcatcgcc cgcgtaccag cccgcctgca gcggggtcac
    20101 gtgggggtcg aatcgctcgc tgagcgtcag gaacgcgacg ccgcgcggcg agcacagcca
    20161 cttgtaggtg tggcagaccg tgacgtcgaa caccgaggca tccacgggga gcacgccggc
    20221 cgcctgcgtg acgtcacaga acgtgtacgt gtggtggcgc gacgccgact cgatgatcgc
    20281 atcgacgtcg gcaacgatgc cgttggcaga ctgaacgagc gagaacacca ccaaccaggt
    20341 gtcgtcggtg atcgaatccg cgagggcggc gagaggcacg ctgcgcacgc ggatgtccgt
    20401 gcgctgcagg aacgggaact cgatcgacga gaagtcgccg tcgggaatca gaacctcagc
    20461 acccgctggc acggcggccg cgatgagcgc ggtgagcacc gacgtctgcg aaccttgcgc
    20521 aacgcgcgac gcgggtacgc cgacaatcga cgcgtaggac tcgcgcgctc gctcgatgct
    20581 ggcgtcgtag ccctgtggat cccggtccgc agcggcccac gccgccaggt cggcggacag
    20641 agcgtcgacg gctgctcgcg ggggcaagcc gatcgacgcc acggcaagat acccgcgggg
    20701 atcaccaaac tcgtcgatgg ccttcgtcag ggcgctggat gtcatgcgct ccagcatcgc
    20761 ggtccggaaa acatgcaaca agggcatgct tgtcatgctt tcaataaccg agcattatgc
    20821 ttccggtcat ggacgcgaat ctcgacctgc actcgatccg gattgtgcgc gccatcgccg
    20881 agcacgggac tatcagcgca gccgcgcgcg cactgggcta cagccagccc gccatcagcc
    20941 agcacctgcg gcggtcggag gcccgcctcg gcacgccgct ggtcatccgt tcgggacgtg
    21001 gcgtgcgtct ctccgaggcc gggcaggtcg tcgctcggca cgccgtcgcc atcacctcag
    21061 ccctggatgc cgccagcgga gatctcgcgg aactggtggg cctcgccgcg ggtacggtgc
    21121 gagttgcggc gttccccacg gcgtcatcca cgctcgtgcc ccggcttctg gccagcatgc
    21181 gccagcggca tccgggcatc acggtcgggt acgtcgaagc cgagccgccc gaggcgatcg
    21241 cgatgctgcg tgacggcgct gtcgacctcg cgatcacctt cgcctatccc ggcgaccggt
    21301 ccgacccgca tcgtccggcc gacgtctccc tcgagtcggt gccgctcttc accgagccag
    21361 tggtgctcgc gctccccgat cgccacgctc tcgcggatcg tcagcaggtg gcactggcag
    21421 accttgccga cgagcgctgg atcgccggat gcccgttgtg ccgcggccac ctgctggcag
    21481 cctgtgaagc cgtgggcgtc accccgaaca tcgagctcga aaccgacaac gccatcgccg
    21541 tgctgaccct cgtcgccgcg ggcctcggcg ttgcgttgct gccgcagctc gcgctggcca
    21601 ccgcacccgt accgcagggc gcgacagttc gctcgacgac cccgtcgagc gatcgcagca
    21661 tccagctcgt cgtgcagaac ggctcgcggc gggtgcccag cattgccgcg accatcgagc
    21721 ttcttgaaca agttgacggg gccgactggg gtcttgcccg agtgccctag tcggccgccg
    21781 agaggctgtg cgcccgctcg aggatacgga cgacctcgag cgcatcacgt ggatcaaccg
    21841 gcgccggcgc tccgtcgcgg atcgcggcgg ccactcccgc gtagaacgcg ggatagtcgc
    21901 caggctcggt cggcaccggg gtcaggcccg cttcggagcc gtcgatgccc agtagacccc
    21961 actcggattc gggggtcacg ccgtagccgt cggatccggg ccacttctgc agtttgagcg
    22021 ccggctcttg gccatcgaga ccgtagaccg tgtaccccga ttcggacccc agcacgcgga
    22081 accggggtcc gctctgggcc gcagcccgac tcatcgtgag atgcgagcgc accccgctcg
    22141 agtgcagaag cgagatgaag gcgtcgtcgt cgctgacggc gccatcccgc acgatctgca
    22201 gctcggcctg ctcgacgacg gcgggaccga acaactgcag ggcctgatcg acgaggtgac
    22261 tgccgagatc gtaggtgatg cccgcgccct gctcgatggt cgtcgtgtcc tgccagcggt
    22321 cgcgattcgg gcctgaccag cgctcgaagg tcgactcgaa acggtgcact gtaccgagcg
    22381 caccctcggc gatcaggcgc ttgacggtga ggaagtcgcc gtcccaccgc cggttctgga
    22441 agacggtgag cacgcggtcg gcatcctcgg ccgcagcgat caacttcttc gcgtcgctga
    22501 cggtcggaac gaagggcttg tcgatgacga ccgccgcgcc cgcctccagt gctgccagac
    22561 cctgctcgag atgagtgtgc gccggcgagg cgaggatgac gagatccaga tccccggcgc
    22621 gagccagcaa ctcgtcggga gtcgcgacga tctcggcccc cggatgctgg gcggcggcct
    22681 cagcggcacg atccgggttc gccgtggaaa tgaggtcgag ccggaaggcc gggttcgtgg
    22741 cgatgaacgg agcgtggaac acgcgaccgg cgaggccgaa cccgatgatc ccggtgcgga
    22801 tgggagtggt ggtggtgggc atccgctcag cctagtgagc tagacgctcg gcgcgtccac
    22861 cgcgccgccg aagcgacggc ttctgctcgc atacagctgg atcgcgtgcc agaggttctc
    22921 ccgggtgaag tccggccaca gcgtgtcgag gaagaccatc tcggcgtagg cgctctgcca
    22981 cagcatgaag ttgctcgtgc gctgctcgcc cgagctccgc acgaacaggt cgacgtcggg
    23041 aaggtcgggc tggtagaggt gcctctggat ggtcttctcg ctgatgccgg tgggagagag
    23101 gcggccggcg gcgacgtccg ctgcgatctc gcgcacggca tccgtgagtt cgttgcggcc
    23161 accgtagttg acgcacatcg tgagcgtgag cacggagttg tgggctgtga gcttctccgc
    23221 gaactggagc tccttgatga cggatgccca gagtcgcggc ctgcgcccgg cccaccgcac
    23281 ccgaactccc cactcgttga gctggtcacg gcggcggtga agcacgtccc ggttgaagcc
    23341 catcaggaat ctgacctcgt caggcgagcg gctccagttc tcggtggaga aggcgtagac
    23401 gctcacgtgc ttcacg
//
LOCUS       DAOSOU010000006        22561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2299_length_22561_cov_7.345997, whole genome shotgun sequence.
ACCESSION   DAOSOU010000006 DAOSOU010000000
VERSION     DAOSOU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 22561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22561
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2299_length_22561_cov_7.345997"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLW15_00725"
     CDS             complement(<1..850)
                     /locus_tag="PLW15_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT32953.1"
                     /translation="MFTFLARRIASGLVLIVVVSAIAFTLVYSDGNRIARVILGPTAS
                     QESVQQRAEELGLNDPLPVQFARWFGGLLRGDLGNSLSDNRPVIETLSLRIPVTLTLV
                     IFTLIVTVIIAVAIGLFSAYKGGWMDSALRIFGVFGFATPHFIIAIALVAVFALALGI
                     LPATGYVTPSKSVEKWIASLILPVTALAVGTIASASQQIRGAVRDVLRQDYIRTLRSR
                     GIPLPAIYLRHALKNASAPGLTTVALQFIGLLGGAVVIERVFALPGLGTLIIDSALKS
                     DIPVIMG"
     gene            complement(929..2470)
                     /locus_tag="PLW15_00730"
     CDS             complement(929..2470)
                     /locus_tag="PLW15_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT32954.1"
                     /translation="MKFRTRIVTAITALAVTSAALVGCSPSAPEPDVERTLTLAVSSD
                     SLWGFVRADLGTQVHQSAAYWKPLYDTIVKITPDLEIQPNLATEWSYNDDLTVLTLKL
                     RDDVKFTDGTPVDAAAVKANLDAFRNGGGGEASQSTGRITDVAAPDATTVVITLSGAD
                     PSVLPGLAESAGAVFLPADLENPDADKSVVPVGSGPYLLDEDNTTPGSVYTYVRNPDY
                     WNPEAYPYDTLVLRPMPDVAARMNALKAGEVDGAAVPAANAADIEASGLTLNTGIANW
                     AGLHILDRNGEVVPALADVKVRQAINMVFDRASIVKNVYQGYGVPNNQAFRDGSAAFV
                     ADETDKYPFDIEAAKKLMAEAGYADGFDITIPAIIGNHDAIMPIITQQLGEIGIRVTV
                     EQFQLGDYFNEVFAKKYGIVFVSLPSFDDWRDVTGMVSPEALWNVFGTEDPALDKLID
                     EARTTTGDQQDAAFQGIGKYLLDNAWYAPWATPNVIYATNDTVSVQLSPGQIEPLIWD
                     IKPID"
     gene            2716..5544
                     /locus_tag="PLW15_00735"
     CDS             2716..5544
                     /locus_tag="PLW15_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT32955.1"
                     /translation="MPTDRVTVTPVRVERNRTSGVSPVPAPHLSWTVTTSEPDWMQAS
                     AELRLDRDGATVQAEVGAQSVLVPWPFDPLVAGSTASIRVRVTSTRGDTSEWSDAVTV
                     TAGFLDPGGWTAPYVGLPAPSKAAQPVLLRGVVDLTQDVRRATLFATARGVYQATVNG
                     VDVDGDRFKPGWTAYQWRVIHESTDVTALLAPGRNVLGARLAGGWYTEEYGFGGGARR
                     FYGDQPALAMQLEVEYVDGSRDTLTTGPAWRATEDGPLRSSSIYLGEHYDATREIPGW
                     GTAEFDDSGWSPVAVLEPGPTPEPRVGPPVRHTGTLPVLSVITTPSGRTVLDFGQNMV
                     GHVRFTASGERGERVTLRHAEVLEHGELGTRPLRRAIQTDQYVLAGGAPETWEPEFTF
                     HGFRYVEVDGWPGALDPTAFEAVVVHSDMTRTGGFECSDDLLNRLHENAVWGMRGNFL
                     SIPSDCPQRDERWGWTGDIQVFGPTASFLFDTDGFLVSWLRDLALEQEHNGGVVPVIV
                     PSPLTVLRGPVAAWGDAASILPWVLDERFADAAAVAAQYPSMRAWAETVIAESDPQTL
                     LWDASFQFGDWLDPDAPADLPGRAKTQPDVVATAHVFRTLTLTARAAERLGENADAQR
                     YAELAERVRLAFLSAYVTPAGRMVSDAPTAYAMAIVYGIAVEPDLRATLGDRLASLVR
                     ASGFHIGTGFVGTPIILDALTATGHVQTAERLLLQTENPSWLYPVTMGATTIWERWDS
                     MLEDGSINPGEMTSFNHYAFGAVADWLHRRLAGLAPDAAGYRRIRIEPLPLPALSFAR
                     AHLDSPYGLIRSGWTRSADGTVVVEAQVPPGTTATVVLPDGRAPFAVGSGSHRWTVDA
                     PASHETLRELSLASDLGEVLDDREAYVAVMTAIRSVAPEGAIRLRRQALWSIRRTLGE
                     SIAFMPGAVTDAVLEALAVLNSRRGPRH"
     gene            complement(5516..7654)
                     /locus_tag="PLW15_00740"
     CDS             complement(5516..7654)
                     /locus_tag="PLW15_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT32956.1"
                     /translation="MNDYLRTRRRLLLDMHIPDWDDRFLADYEPAELARLARQAGVEG
                     ALIYCKSHLGLNYWPSPVGGIHRAASARDLVAEAKSRFEAEGISVGAYHSVIFDNWAA
                     DNHPEWRVVPATREAGYDWTVLGHRYGTLCPNHRGYREYEEQQISALLDRYDFDVLWI
                     DMAFWTAVCVCDACEARYRQEFGVPIPRVIDWSSVEWTRFQSARERWLAEFVAELVAA
                     AKSGRPALAVTHNLAPGLRDWSRGQRAEDAAVDDFAAGDLYGGFDEQVFVIALMRGLR
                     PGVLTEFMTTRTRDLFTHTSTKTEHEMTTQVLIAVAADSAFLFIDAVEPSGRLNQGVY
                     QRMGRAFAAALPFEGELGGEEITDVAVYYSDDSRIDEADDGSPVGAVHAGGSRHQDAV
                     MGAVRVLRRAHIPFSVVTRLDNARLARFPVLLVPDARRLSRADIDALRAYVAAGGRLY
                     ASGRTSLLLSDGTALADFALADVFGCHAIASEEGSTVSLRPETDTLRAAASPELTVPV
                     GRRQPAPARPGPSLGLPRIAANDSTRVLSRLSLPFGYPSDGTRAGRDFASIHSSPPWE
                     HTAHPAIVAADYGAGRSVYSIAPIEAEGGPQHDDTLVALLVGELGLAPRVTAVGHPDV
                     WITAFHQPDRSRMLVSALRLAEDPPEGSVPVTIRARPPRGTTVVGVRDVESGEPLSWR
                     WDDLVVVIPAAVELFAMVAIELSAEDPSAS"
     gene            7835..9007
                     /gene="rhaI"
                     /locus_tag="PLW15_00745"
     CDS             7835..9007
                     /gene="rhaI"
                     /locus_tag="PLW15_00745"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOT32957.1"
                     /translation="MSATLPSEVLEALEAQQIELPSWAFGNAGTRFKVFSTPGTPRTP
                     EEKISDAAQVHRVTGLAPAVALHIPWDRVDDYAALSAYAVDHGLRIGTINSNTFQDDA
                     YKFGSLTHVDKAVRQMAIDHHFECIDVMHQTGSRDLKIWLADGTNYPGQGDIRGRQDR
                     LADSLHAIYERLGADQRLVLEYKFFEPAFYHTDVPDWGTAYAQVVALGERAVVCLDTG
                     HHAPGTNIEFIVAQLLRLGKLGSFDFNSRFYADDDLIVGAADPFQLFRILYEVIRGGG
                     YGVDSDVAFMLDQCHNVDDKIPGQIRSVLTVQEMTARALLVDRAALTVAQDSGDVHTA
                     NGLFMDAFYTDVRPALADWREQRGLPRDPLAAYAASGYADAIIAERVGGTQASWGA"
     gene            9016..9888
                     /locus_tag="PLW15_00750"
     CDS             9016..9888
                     /locus_tag="PLW15_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOT32958.1"
                     /translation="MTVSDTTIDGPHGPLLLRIYTPDRPHGPALVWVHGGAYAFGDVS
                     MPESDWVSRQLAERGVAVVAVDYRLSPTIDLTDASVAPSGDGVHFPVASEEVVAAFHW
                     MLAHANQLGADPEQLFLGGGSAGANLALSAMLRLRDGGEQHPRAGLLVYPSVHAVVPP
                     LTRELEAGLGDLPPILRFSPEVMRVIHLNYVGDEAELTNPYAFPGGHDLRGLPPVFIL
                     NSDNDPLRASGEALAGELAAAGVDTLVMREVGTRHGHLNSPDEPLALRSIDRMAAWVH
                     GGPFLGVRHEASRT"
     gene            9922..10923
                     /locus_tag="PLW15_00755"
     CDS             9922..10923
                     /locus_tag="PLW15_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT32959.1"
                     /translation="MATIRDVATLAGVSAGTVSNVLNRPSYVRQETRDRVLAAIRQLD
                     FVPDQGSRQFRPGRVRTLGLAIANLDNPFFVDVALGAEERCKELGVGVVICNSGYDPG
                     REVQNLDLLVQSRVQGIIITPVDENSSRLELLRDRGVPMVFVDRVGDDRDCWSVVVDD
                     VEGGRLGAQHLLDRGHRRLAFLGHPDRSPKVGARLSGARGALTGRPDATLELITSESW
                     TIEAGQSAARALAARAPAQRPTAVLCANDMLALGLEQQLLRDGIQVPGDVAIVGYDDL
                     MWASAASVPLTTVRQPRQVLGRTAVDMIMELLAGPETGAARRNHVVLQPELVTRESA"
     gene            complement(10904..11899)
                     /locus_tag="PLW15_00760"
     CDS             complement(10904..11899)
                     /locus_tag="PLW15_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOT32960.1"
                     /translation="MARRTGILAQVARIDRLVEDDGPARGARRIRLVTGGGLEVEIHP
                     DRALDLGQVTYRGTPIAWMSPTGMVAPAIAECTGDSWLRSFGGGLLATCGLDTYGPAT
                     TDAGVQFSLHGRVGTVPALVTRSSISGTELVVSGLVRQASVFGENLVLERTWSADLGG
                     TSLRLTDVVRNEGLEDSGHMVLYHVNIGWPLLDESAVLDIPSLEVTAKDGAASADPVG
                     WQTIEAPQHGYRERVYSHRFVGQGTVRVAIDNAELDVRFELGFDSSTLPALHQWKMSG
                     EGHYVMGLEPVNVSTFGGRAGARAAGILPVLPRGGEVSYTMEFGFGPSQLTRSRA"
     gene            complement(11941..13206)
                     /locus_tag="PLW15_00765"
     CDS             complement(11941..13206)
                     /locus_tag="PLW15_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32961.1"
                     /translation="MTKAGPYDGVTSAEGSIERAMEAGGGVLRLAPAWVPRSFCTPGR
                     RLRLHPDDYFPLGKERGGIDERWFASAIRAENGPLTDPYEGLSLVVGADGEVIPFDEF
                     IAHLKAEAIGPLWGEYGTWPMYSKFFDNQFPLPFHLHQKDEHAALVGKRGKPEAYYFP
                     PQLNNHGGEFPYTFFGLHPETTRQQVLDKLEAFLRGGDNRITELSSAHRITPGTGWDV
                     PPGVLHAPASMCTYEPQAASDVFAMTESWSNNREVADELLWKDVPADRVGDLEFILEI
                     IDWDLNVDPEFWSHRFTPPVETDASAAAGRDRYVERWITYRSPAFSAKELTVAPGCEV
                     SIDESDAYGFIAVDGFGEINGQEVSAISSIRYGQLSHDEYFVTSSAAQAGVRIRNTSP
                     SADLVLLKHFGPGNRELAADRGAVAAERA"
     gene            13325..14074
                     /locus_tag="PLW15_00770"
     CDS             13325..14074
                     /locus_tag="PLW15_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOT32962.1"
                     /translation="MSPLLGTERRELLLSILGRAGVIRLDTAAEELGVSVMTVRRDLT
                     DLEGEGLVRRVRGGAVAPILPEPFSARAASRSAAKSEIARKALALVPSRGAVAFDAST
                     TSGAVLAGVDGGDLMVSTNSVENAAAARRRPGVRSVLVGGELEQRTGSFVGPIAHLGA
                     RSLSYAVFFSSASAVDAEFGPSEASLAEVEVKRCFAEQADRTVLLVDASKLGLRALAR
                     GLDWQSIDLLITELPAADERLDPYRDLVELR"
     gene            14121..16172
                     /locus_tag="PLW15_00775"
     CDS             14121..16172
                     /locus_tag="PLW15_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HOT32963.1"
                     /translation="MATTPSDLIARSNRLGSDPRVTNYAGGNTSAKGTEIDPVTGEPV
                     ELMWVKGSGGDLGTLTESGLAVLRLDRLRALVDVYPGLDREDEMVAAFDYTLHGKGGA
                     APSIDTAMHGLVDAAHVDHLHPDSGIAFATAKDGEKLTKKAFGDKVAWVDWRRPGFQL
                     GLDIAAIKAANPQAVGVILGGHGITAWGETSKEAEQNSLWIIRTAEEYIAAKGRKNPF
                     GATVKKNVALPVAERRAKAAALAATIRGIASHDRPMVGHFTDSDVVLDFLASASAPRL
                     AALGTSCPDHFLRTKVKPLLLDLPANASVEASIARLKELHEAYRADYTAYYEKYAEVS
                     TSSTTGSPPMRGADPLIVLVPGVGMFSYGANKQTARVAGEFYVNAINVMRGAEAISSY
                     APISDAEKFRIEYWALEEAKLARMPKPKSHATRVALVTGAASGIGKAIATRLAADGAC
                     VIIADLDLAKAQAAAAEIGDTDVAIGLQVNVTDAAAIATMVQDAVLAFGGLDIVVNNA
                     GVSLSKPLLETTEQDWDFQHDIMAKGSFLVSKATAPVLIEQGMGGDVIYISSKNSVFA
                     GPNNIAYSATKADQAHQVRLLAVELGEHGIKVNGINPDGVVRGSGIFAAGWGANRAKT
                     YGIDEQDLGKFYAQRTILKREVLPENVANAVSVLTGPDLSHTTGLHIPVDAGVAAAFL
                     R"
     gene            16169..17596
                     /locus_tag="PLW15_00780"
     CDS             16169..17596
                     /locus_tag="PLW15_00780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOT32964.1"
                     /translation="MNGSRRPRSPGTVAAVDLGATSGRVMLGHVDHNELSVRPVARFP
                     NGPVQRADGLHWDIDALYSHALAGLAAALREEPTLAGIGIDSWAVDYTLLTGGRMLGL
                     PFHYRDERTARGVERVHSVVSPEELYAANGLQFLPFNTLYQFAAEGNLDTVDSFLLIP
                     DLLAYWLTGRRVAERTNASTTGLLGVATGQWDDALIERLALPRRIFPELVDPGTPLGS
                     LRPEAAAIVGGDLSVTAVGSHDTASAVVAVPAASDDFAYISCGTWGLVGVELETPVLT
                     EASRLANFTNEGGVDGRVRYLHNVMGLWLLSESVRQFNDEGKRDLDLPELLAAASALT
                     TPVSVFDANDASFLPPGDMPARIAAWCTTHGLAAPQTPVEFVRSIIESLAVAFADAVR
                     TASELSGKTVSVIHIVGGGSQNELLCQLTADRSGLPVLAGPVEATAIGNVLVQARALG
                     FASGSLESLRSLVAGAFLPVLYRPR"
     gene            complement(17593..18432)
                     /locus_tag="PLW15_00785"
     CDS             complement(17593..18432)
                     /locus_tag="PLW15_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT32965.1"
                     /translation="MRIAVTGGNGKLGRVTVEVLRAAGHEVVVLDQAGPDRSAFTQLD
                     ITDYGQVIDALAGINDRHGGVDAIAHLAAIPAPGILTDAATFHNNVVGTFNVFQAARR
                     LGIRRIAFASSETVLGLPFDAPPPYVPLDEEYDTRPESVYSLGKHLEEELARKIARWD
                     PELSITALRFSNVMVDADYAEFASFTAESRKWNLWGYIDARDGAEAIRLALEAGTPGY
                     DVFVIFAGDTVLERPNAELMAEFFPGVEVRGELGVNDSLTSTAKARRILGWKPKHSWR
                     DAR"
     gene            18508..19146
                     /locus_tag="PLW15_00790"
     CDS             18508..19146
                     /locus_tag="PLW15_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT32966.1"
                     /translation="MTVEPTPVLPDTIADELRASILSQVDAPGSALTESAVALRFGVA
                     RPTARLAIDKLVADGLLRREAHRAARVPELSRDDIVDLFDNRAIIESAAMAALATAGT
                     IPAAAVAAHRALTTTEDFAQHDIAFHRALVAGQPSPRLTRLHELLMGEIELCIGQVQA
                     AHLLSSAEIARQHQGILDAITSGDADAAARLTRDHIVGARDRLLARTTKGPQ"
     gene            19143..20477
                     /locus_tag="PLW15_00795"
     CDS             19143..20477
                     /locus_tag="PLW15_00795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOT32967.1"
                     /translation="MTKRQFPNPVELLKLMKFKKPTFNAKKRRLSGALTIDDLRLIAK
                     RRTPKAAFDYTDGAAEGELSMSRSRQAFEDVVFHPSILRDVSTVDTSTTILGGTSAMP
                     FGIAPTGFTRLMQTEGEIAGAGAAGAAGIPFTLSTLGTTSIEDVKAANPDGRNWFQLY
                     VMRQREISYGLVERAAAAGFDTLMFTVDTPVAGARLRDKRNGFSIPPQLTLGTIVNAI
                     PRPWWWWDFLTTPPLEFASLSSTGGTVGELIDSAMDPSISFEDLKIIRGMWPGKLVVK
                     GVQNVEDSKTLADLGVDGILLSNHGGRQLDRAPIPFHLLPEVVREVGKDTTVMVDTGI
                     MNGADVVASLALGAKFTLVGRAYLYGLMAGGRAGVDRTIQILSEQVVRTMKLLEVANV
                     EELGPQHVTQLERLVPRAVSPALTSAVPASKRTVVRTPAAKPTAAKAPARRS"
     gene            20532..21704
                     /locus_tag="PLW15_00800"
     CDS             20532..21704
                     /locus_tag="PLW15_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32968.1"
                     /translation="MSWVYPVSEAARTTRLGVVAAFAWTGVALLAAGAAATLVVGLSE
                     SGVVPWLALGGFVLAAGNLIVSRRFESGGPVPLAAGAASILACWMVAAGIRLTDLPFD
                     GYVGFPLFAVGVAATLVGAIADRWSGAIGTLIGFVAGQAALVLAYQGAVPAGLVIDPA
                     TAAVAVIVALFISMLSVVRRRARASGPRLDDSGRRDLTAHERQSLLLNSRALVHDTVL
                     GELAALGMTRPGPLTQSMATSIRASLVAAASAAGPSPEPGPPPVALADLLTRFRIAGL
                     HITVTGDPSALDRLSADRRASLVLAMEQCLVNVLQHSGVTHAELSVAVSEGRLITTVV
                     DEGSGFDEGAVPADRFGLRESIRGRIADLGGAVRVLTGAGTGTSVIVSVPIDGDST"
     gene            21701..>22561
                     /locus_tag="PLW15_00805"
     CDS             21701..>22561
                     /locus_tag="PLW15_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32969.1"
                     /translation="MSARATGRQASQQRATPQRLDPLGGLTAQYFIWSVAIIAVAVAV
                     TQVVRHANDVRSPALEAIALLCIVAAGGAVVDGAAARRFPFSAQRHAVLHVLGVLAVA
                     FDLAGRDHGAGESAAWGAVCLAILLMTIGSYRPAAEILLMTAVSSITVAALVVGFSAG
                     LTPAQVALFAITRAAPIAAVGAGAAAFSNTLVTRLTQWQNGAAERAEIARATVREELL
                     AEVSNARHELLEHRAAPFLQDVLDRGVVTPADAARARGLATALRRTMLAEAGRGWLVE
                     LADTVDDPEKV"
BASE COUNT         3470 a         7750 c         7739 g         3602 t
ORIGIN      
        1 tccccatgat cacggggatg tctgacttga gggccgagtc gatgatgagg gtgcccagtc
       61 cgggcagggc gaagacacgc tcgatgacta cggcgccgcc gagcagtccg atgaattgga
      121 gggccacggt ggtcagtccg ggtgcgctcg cattcttgag tgcgtggcgc aggtagatgg
      181 cgggcagtgg gatgccgcgg ctgcgcagcg tgcggatgta gtcctggcgc agcacatcgc
      241 gaacggcgcc ccggatctgc tgggacgcgg acgcgatggt tcccaccgcg agcgccgtga
      301 cggggaggat gagcgacgcg atccacttct ccaccgactt gctgggagtc acgtatccgg
      361 tggcggggag tattccgagc gccagtgcga agaccgccac gagcgcgatc gcgatgatga
      421 agtgcggcgt cgcgaatccg aagactccga agatgcgcag tgcgctgtcc atccagccgc
      481 ccttgtaggc gctgaagagg ccgatcgcca ctgcgatgat gaccgtgacg atgagtgtga
      541 agatcacgag tgtgagggtg acggggatgc gcagcgacag cgtctcgatc accggacggt
      601 tgtcactgag cgagttgccc aggtcgcccc gcagcagccc cccgaaccag cgggcgaact
      661 gcaccggaag cgggtcgttg aggccgagtt cctctgcccg ctgctgcacc gactcctgcg
      721 atgcggtcgg accgaggatg acccgggcga tccgattccc gtcgctgtag acgagggtga
      781 acgcaatggc tgacaccacg acgatcagaa cgagtcccga ggctattcgg cgggccagga
      841 acgtgaacat gggggactcc aactactcgg ttaggtacaa ggatgatgcc ccgccgcggg
      901 aaactcccgc ggcggggcat ccctggtgct agtcgatcgg cttgatgtcc cagatcagcg
      961 gctcgatctg gcccgggctc agctgcaccg acacggtgtc gttcgtcgcg tagatgacgt
     1021 tcggcgtcgc ccacggggcg taccacgcgt tgtcgagcag gtacttgccg atgccctgga
     1081 atgcggcatc ctgctggtca cctgtcgtgg tgcgggcctc gtcgatcagc ttgtcgagcg
     1141 ccgggtcctc ggttccgaag acgttccaca gcgcctcggg ggagaccatt cccgtcacgt
     1201 cgcgccagtc gtcgaagctc ggcagcgaga cgaacacgat gccgtacttc ttggcgaaga
     1261 cctcgttgaa gtagtctccg agctggaact gctcgacggt gacgcggatt ccgatctcgc
     1321 ccagctgctg cgtgatgatc ggcatgatcg cgtcgtggtt accgatgatc gcggggatcg
     1381 tgatgtcgaa cccgtcggcg taaccggcct cggccatgag cttcttggcg gcctcgatgt
     1441 cgaacgggta cttgtcggtc tcgtcggcga cgaacgcggc gctgccgtca cggaacgcct
     1501 ggttgttcgg caccccgtag ccctggtaga cgttcttcac gatcgaggcg cggtcgaaga
     1561 ccatgttgat cgcctggcgc accttgacgt cggcgagcgc cggaacgacc tcaccgttgc
     1621 ggtcgaggat gtgcaggccc gcccagttgg cgatacccgt gttgagcgtc agtccactcg
     1681 cttcgatgtc cgcggcgttg gcggcgggca cggcggcacc gtcgacctcg ccagccttca
     1741 gcgcgttcat gcgagcagcg acatccggca tcgggcgcag cacgagggtg tcgtacgggt
     1801 aggcctcggg gttccagtag tcggggttgc gcacgtaggt gtagaccgag cccggcgtgg
     1861 tgttgtcctc gtcgagcagg tacggacccg agccaaccgg cacgaccgac ttgtcggcgt
     1921 ccgggttctc gaggtcggcg ggcaggaaca ccgcgccagc gctctcggcg agtccgggca
     1981 gaacgctcgg gtcggctccg ctcagcgtga tcacgacggt cgtcgcatcc ggtgcggcca
     2041 cgtcggtgat gcgtcccgtg ctctgggatg cctcgccgcc accgccgttg cggaacgcgt
     2101 cgaggttcgc cttgacggcc gctgcgtcga cgggggtgcc gtcggtgaac ttgacgtcgt
     2161 cacgcagctt gagcgtgagc accgtcaggt cgtcgttgta gctccactcg gtagccaggt
     2221 tgggctggat ctcgaggtcg ggcgtgatct tgacgatggt gtcgtacagc ggcttccagt
     2281 aggcggccga ctggtgcacc tgggtcccca ggtcggcgcg tacgaagccc cacaggctgt
     2341 cggacgaaac cgcgagggtg agcgtgcgct cgacgtcagg ttcgggcgcg ctcggagagc
     2401 accccactag tgctgctgat gtgacggcaa gggcggtgat cgccgtcacg attctggtgc
     2461 ggaacttcat tgttgcctct tctcactcta cgactctgtt cggggagatc cggtgctatg
     2521 aatcgtttca atcaccggtg aatgttgaca atacatcccg accagccgct tgtcgagttc
     2581 gagttatggt cgtctcccgt cgcccgtcgc gcagccgcga ctctccgccg aactgggtct
     2641 tgaaacgatt catcacgatt cggtatatcg tgtgcgaggt cagggctacc ggcgccccgg
     2701 aacgaggagg accacatgcc gacggaccgc gtgacagtga cgccggtgcg agtcgagcgc
     2761 aatcgcacgt cgggcgtcag ccccgtgccc gctcctcacc tcagttggac ggtcacgacg
     2821 tcggagcccg actggatgca ggcctctgca gagctccggc tcgaccgtga cggcgccacc
     2881 gtgcaggcgg aggttggtgc gcagtcggtg ctggtcccgt ggcccttcga tcccttggtc
     2941 gccggcagca ccgcgagcat ccgggtacgc gtgacgagta cgcgaggcga cacctccgag
     3001 tggagcgacg ccgtcacggt caccgccggc ttcctcgacc cgggcggctg gacggcgccg
     3061 tatgtcgggc tccccgcacc ctcgaaggcg gctcagccgg tgctgctccg aggtgtcgtc
     3121 gacctgactc aggacgtgcg cagggcaacg ctgttcgcga cggcgcgcgg cgtgtaccag
     3181 gccaccgtca acggcgtcga cgtcgatggg gatcgcttca agcccgggtg gacggcgtac
     3241 cagtggcgag tcatccacga gtcaacggat gtcacggccc tgctcgcacc cgggcgcaac
     3301 gtgctcgggg cccggctggc cggcgggtgg tacaccgagg agtacggatt cgggggcggc
     3361 gcccgacgct tctacggcga ccagcccgca ctcgcgatgc agctcgaagt cgagtacgtc
     3421 gacggatcgc gggacacgtt gacgacggga cccgcgtggc gcgccaccga ggatggcccg
     3481 cttcgctcca gcagcatcta cctcggagag cactacgacg ccacccgcga gataccgggc
     3541 tggggcacag cggagttcga tgactcgggc tggtcgcctg tcgccgtgct ggagccgggc
     3601 cccacgcccg agccccgagt cggaccaccg gtgcgccaca cgggcaccct cccggtgctg
     3661 tcggtcatca cgaccccgtc gggtcgcacc gtgctcgact tcgggcagaa catggtcggt
     3721 cacgtgcgct tcaccgcgag cggcgagcgc ggcgagcggg tgaccctccg ccacgccgag
     3781 gtgctcgagc acggcgagct gggcacgcga cccctgcgac gagcgatcca gaccgaccag
     3841 tacgtgctcg ccggcggggc acccgagacc tgggagcccg agttcacctt ccacggcttc
     3901 cgctacgtgg aggtcgacgg ctggccggga gcgctcgacc cgacagcgtt cgaggcggtc
     3961 gtggtgcaca gcgacatgac gcgcacgggc ggattcgagt gctccgacga cctgctcaac
     4021 cgcctgcacg agaacgccgt gtggggcatg cgcgggaact tcctgtccat ccccagcgat
     4081 tgtccgcaac gcgacgagcg gtggggatgg accggcgaca tccaggtgtt cggcccgacc
     4141 gcgagcttcc tcttcgatac cgatggtttc ctggtctcct ggctgcgcga cctcgcgctc
     4201 gagcaggagc acaacggcgg ggtggtcccc gtgatcgtgc cgtcgcctct caccgttctg
     4261 cgcgggccgg tggccgcgtg gggcgatgcg gcgagcatcc tgccgtgggt gctcgacgag
     4321 cgcttcgcgg atgcggcggc cgtcgcagca cagtacccga gcatgcgcgc ctgggccgag
     4381 accgtcatcg ccgagagcga cccgcagacc ctgctctggg atgcgagctt ccagttcggc
     4441 gactggctgg acccggatgc cccagccgac cttcccggcc gcgccaagac ccagcccgac
     4501 gtggtggcca ccgctcacgt cttccgcacg ctgacgctca ccgcgagagc cgcggagcgg
     4561 ctgggcgaga acgccgacgc tcagcggtac gccgagctgg ccgagcgcgt gcgcctcgcc
     4621 ttcctgtccg cgtacgtgac ccccgcggga cgtatggtct ccgacgcccc caccgcctac
     4681 gcgatggcga tcgtctacgg catcgccgtc gagcccgact tgcgcgccac gctcggcgac
     4741 cggctggcga gcctggtgcg ggccagtggc ttccacatcg gaaccgggtt cgttggtacc
     4801 cccatcatcc tcgacgcgct caccgcaacc ggtcacgtgc agacggccga gcgcctgctc
     4861 ctgcagaccg agaacccgtc gtggctctac ccggtgacca tgggtgcgac cacaatctgg
     4921 gaacgctggg acagcatgct cgaggacggc tcgatcaacc cgggcgagat gacctccttc
     4981 aatcactacg ccttcggcgc tgttgccgac tggctgcacc gccggctcgc cggcctggcc
     5041 ccggatgccg cgggctaccg gcggatccgc atcgagccgc ttccgctgcc cgcgctgagt
     5101 ttcgcccggg cgcacctgga ttcgccatac ggcctcatcc gatcggggtg gacccgatcc
     5161 gcggacggca ccgtcgtcgt tgaggcgcag gttcccccag gcacgaccgc aacggtggtt
     5221 ctgccggatg ggcgtgcgcc gttcgcggtg ggctccgggt cgcaccgctg gacggtggac
     5281 gcccccgcct cccacgagac cctgcgcgag ctctccctcg cgagcgacct gggcgaggtg
     5341 ctcgacgacc gcgaagcgta cgtcgcggtg atgacggcca tccgctccgt cgcgcccgag
     5401 ggggcaatcc ggctgaggcg tcaggcgctc tggtcgatta ggcgcacact cggcgaatcg
     5461 atcgcgttca tgcccggagc ggtcaccgat gcggtgctcg aggcactcgc ggtcctcaac
     5521 tcgcggaggg gtcctcggca ctgagctcga tcgcgaccat ggcgaacagt tcgaccgcgg
     5581 ccgggatcac gacgacgagg tcgtcccacc gccacgacag cggctcgccg gactcgacgt
     5641 cgcgaacgcc gacgacagtg gtgccgcgcg gtggacgtgc ccggatcgtc accggcaccg
     5701 agccctcggg agggtcctcg gcgaggcgca gggctgagac cagcatccgg gaacggtcgg
     5761 gctggtggaa ggcggtgatc cagacatccg gatgcccgac cgccgtcacg cgcggtgcga
     5821 ggccgagctc accgaccagc agggccacga gtgtgtcgtc gtgctgggga ccgccctccg
     5881 cctcgatcgg agcgatcgag tacaccgacc ggcccgcccc gtagtccgcc gccacgatgg
     5941 cgggatgagc ggtgtgctcc cacggtggcg acgagtggat gctggcgaag tcgcggcccg
     6001 cgcgcgtgcc gtccgaggga tacccgaacg gcaggctcag gcgggagagc acgcgggtcg
     6061 agtcgttcgc agcgatgcgg ggcagaccca gcgacggacc cgggcgcgcc ggtgccggct
     6121 ggcgacgacc gacgggcacg gtgagctccg gggaagcggc cgctcgcagg gtgtcggtct
     6181 ccgggcgcag cgagaccgtg ctgccctcct ccgacgcgat ggcgtggcat ccgaagacat
     6241 cggcgagagc gaagtccgcg agcgcggtgc cgtccgagag cagcaacgat gttcgaccgc
     6301 tcgcgtagag ccggccgccg gcggcgacgt aggcccgcag tgcgtcgatg tccgcgcggg
     6361 agaggcggcg ggcatccgga accaggagca ccgggaatcg cgccagccgg gcgttgtcga
     6421 ggcgggtcac cacagagaac gggatgtgcg ctcggcgcag cacgcgaacc gcccccatga
     6481 cggcgtcctg gtggcgcgat ccgccggcgt gcaccgcacc gaccggactg ccgtcgtcgg
     6541 cctcgtcgat gcgggagtcg tcggagtagt acacggcgac gtcagtgatc tcctcacccc
     6601 cgagctcacc ctcgaacggc agggcagcag cgaatgcgcg acccatgcgc tggtagaccc
     6661 cctggttgag tcgaccgctg ggttcgacgg cgtcgatgaa caggaaggcc gagtcggcgg
     6721 cgactgcgat gagcacctgg gtggtcatct cgtgttcggt cttcgtgctc gtgtgggtga
     6781 acaggtcgcg ggtgcgggtg gtcatgaact cggtgagcac gccgggtcgc aacccccgca
     6841 tcagggcgat gacgaacacc tgctcgtcga agccgccgta gaggtcgccc gcggcgaagt
     6901 catcgaccgc ggcatcctcg gcgcgctgac cgcgcgacca gtccctcagg ccgggggcga
     6961 gattgtgggt cacggcgagg gcgggccggc cggacttggc cgccgctacc agctcggcca
     7021 cgaactcggc gagccaccgt tcgcgggcgg actggaacct ggtccactcc acgctggacc
     7081 agtcgatgac gcgcgggatg ggaaccccga actcctggcg gtagcgcgcc tcgcacgcgt
     7141 cgcacacgca caccgccgtc cagaaggcca tgtcgatcca cagcacgtcg aagtcgtagc
     7201 gatccaacag tgccgagatc tgctgctctt cgtactcccg gtagccgcga tggttcggac
     7261 agagtgtgcc gtaccgatgt cccagtaccg tccagtcgta gccagcttcg cgcgtcgcgg
     7321 gaacgacacg ccactcggga tgattgtcgg ccgcccagtt gtcgaagatc acgctgtggt
     7381 acgcgccgac gctgatgccc tccgcctcga accgggactt cgcctcggcg acgaggtcgc
     7441 gcgcactcgc cgcgcgatgg atgcctccca ccggggaggg ccagtagttc aggccgaggt
     7501 gcgacttgca gtagatcagg gcgccctcga caccggcctg gcgagcgagt cgggcgagct
     7561 cggcgggttc gtagtcggcc aggaagcgat cgtcccagtc ggggatgtgc atgtcgagca
     7621 gtagtcgacg gcgagtccgg aggtagtcgt tcaccggctg tccgtctcgg tgccgcgcgg
     7681 tcgcggcgaa cccggaacgc ggcctagcgc cctcgtcgca tcgccggtgg cgatgcgacg
     7741 gaatcctgct atagtccgtt gattgaaacg attcacaacc attgtcgtgc cagcataccg
     7801 aggttcgacg aggtctgtcc cgaaaggatg ctccgtgagc gcgacgttgc cctccgaagt
     7861 gctcgaggcg ctcgaggccc agcagatcga actgccctcc tgggcgttcg gcaatgccgg
     7921 aacccggttc aaggtcttca gcacgcccgg cacgccgcgc acgcccgagg agaagatctc
     7981 ggatgccgca caggtgcatc gagtcaccgg gcttgccccg gcggtcgccc tgcacatccc
     8041 ctgggaccgg gtcgatgact acgccgccct gtcggcctac gccgtcgatc acggactgcg
     8101 catcggcacc atcaactcca acaccttcca ggatgacgcc tacaagttcg gatcgttgac
     8161 ccacgtcgac aaggccgtac gtcagatggc gatcgaccac cacttcgagt gcattgacgt
     8221 catgcaccag accgggtcgc gcgacctcaa gatctggctc gcggacggca cgaactaccc
     8281 gggccagggc gacatccggg gacgccagga tcgtctggca gactcgctcc acgcgatcta
     8341 cgagcggttg ggtgctgatc agcgtctggt gctcgagtac aagttcttcg agccggcttt
     8401 ctaccacacc gacgttcccg actggggcac cgcctatgcc caggtcgtcg cgctcggcga
     8461 gcgcgcggtt gtgtgcctcg acaccggcca ccacgccccc ggcaccaata tcgagttcat
     8521 cgtggctcag ttgcttcgac tgggcaagct gggatccttc gacttcaatt cccggttcta
     8581 cgccgacgac gacctcatcg tgggggcggc cgatccgttc cagctcttcc ggatcctcta
     8641 cgaggtgatc cggggtggcg gttacggggt cgacagcgac gtcgcattca tgctggacca
     8701 gtgccacaac gtcgatgaca agatccccgg ccagatccgt tcggtgttga ccgttcagga
     8761 gatgacggcg cgggcgctgc tcgtggacag agccgctctg acggtggctc aggactccgg
     8821 cgacgtgcac acggcgaacg gcctgttcat ggatgccttc tacaccgatg tgcggccggc
     8881 cctcgccgac tggcgcgagc agcgcggtct gccccgcgac ccgcttgccg cctacgcagc
     8941 ctccggctat gcggatgcga tcatcgccga acgcgtcgga ggaactcaag ccagctgggg
     9001 ggcatgatcg tgaacgtgac ggtatcggac accacgatcg acggccccca cgggcccctc
     9061 ctcctgcgca tctacacccc cgaccgcccg cacggtcccg ccctggtctg ggttcacggc
     9121 ggcgcctacg ccttcgggga cgtgtcgatg ccggagtccg actgggtatc ccggcagctg
     9181 gccgagcgtg gtgtggccgt cgtcgccgtc gactaccgtc tctcccccac gatcgacctc
     9241 accgatgcga gcgttgcgcc cagcggtgac ggggtgcact tccccgtcgc atccgaagag
     9301 gtggtcgccg ccttccattg gatgctcgcc cacgcgaatc aactcggcgc cgatcctgag
     9361 cagctcttcc tcggcggggg tagcgcggga gccaacctcg cgctctcggc gatgctccgc
     9421 ctccgcgacg ggggcgagca gcaccctcgt gccggtctgc tcgtctaccc gtccgtgcac
     9481 gccgtggtcc cgcccctcac gcgcgagctc gaggccgggc tcggcgactt gcctccgatc
     9541 ctgcgattct cgccggaggt gatgcgagtc atccatctca actacgtcgg cgacgaagca
     9601 gagctcacga acccgtacgc cttccccggc gggcacgacc ttcgtgggct gccgccggtg
     9661 ttcatcctca actcggacaa cgaccccctg cgcgcttcgg gtgaggctct ggcgggcgag
     9721 ctcgccgccg cgggcgtcga cacactggtc atgcgcgaag tgggaacccg acacgggcac
     9781 ctgaacagtc cggacgaacc gctcgcgctg cgcagcatcg atcgcatggc cgcatgggtg
     9841 cacggcggcc ccttcctcgg cgtgcggcac gaggcatccc gaacctagac tggttcactg
     9901 aacacaggcc cgaggagacc catggcgacg atcagggatg tcgccacgct cgccggagtc
     9961 tcggcgggca cggtcagcaa cgtactcaac cgtccgtcct atgtgcgcca ggagactcgg
    10021 gaccgggtgc tcgccgcgat acgccaactc gacttcgtgc ccgaccaggg ttcccgtcag
    10081 ttccggcccg gccgggtgcg cacactcgga ctcgcgatcg ccaacctcga caacccgttc
    10141 ttcgtcgacg tcgctctggg cgcggaggag cgctgcaagg agttgggggt gggtgttgtc
    10201 atctgcaaca gcgggtacga ccccgggcgc gaggtgcaga acctcgacct gctggtgcag
    10261 tcccgggttc agggaatcat catcaccccc gtcgacgaga acagctcgcg cctcgagttg
    10321 ctgagggatc gcggcgtgcc catggtgttc gtcgatcggg tcggggatga cagggactgc
    10381 tggtcggtgg tcgtcgatga cgtcgagggc ggccgcctgg gcgcgcagca tctgctggat
    10441 cggggtcacc ggagacttgc gttcctcggc catcccgatc gatcgcccaa ggtgggtgcc
    10501 cggctctcgg gggcgagggg cgccctcacc gggcgaccgg atgccactct cgagctcatc
    10561 acgtctgagt cctggacgat cgaggcggga cagagcgccg cgcgggcgct cgccgcgcgt
    10621 gccccagcac agcgacccac agcggtgctg tgcgcgaacg acatgctcgc cctggggctc
    10681 gagcagcagc tgctccgcga tggcattcag gttcccggcg atgtcgcgat cgtgggctac
    10741 gacgacctga tgtgggcctc cgctgcaagc gtgccgctga cgacggtgcg ccagccgcgt
    10801 caggtgctcg gtcgaaccgc ggtggacatg atcatggagt tgctcgccgg gccggagacc
    10861 ggcgcggcga ggcggaacca cgtcgtgctg caacccgaac tcgtcacgcg cgagagcgcg
    10921 tgagctgcga gggcccgaac ccgaactcca tcgtgtagct gacctcaccg ccgcgcggga
    10981 gcacgggcag gattcccgct gcccttgcgc ccgcgcgccc tccgaacgtg ctcacgttga
    11041 ccggctccag acccatgacg tagtgcccct cgccgctcat cttccactgg tgcagggctg
    11101 gcagggtgga ggagtcgaag cccagctcga atcgaacgtc gagctcggcg ttgtcgatcg
    11161 cgacgcgcac ggttccctgc ccgacgaagc gatggctgta cacccgctcg cggtagccgt
    11221 gctggggtgc ctcgatcgtc tgccagccga cggggtcggc cgacgctgcg ccgtccttcg
    11281 ccgtgacctc gagcgagggg atgtcgagca cggcggattc gtcgagcagc ggccatccga
    11341 tgttgacgtg gtagagcacc atgtgcccgg agtcctcgag cccctcgttg cgaaccacat
    11401 cggtgagtcg cagcgatgtt ccacccaggt cagcgctcca ggtgcgctca agcacgaggt
    11461 tctcgccgaa gacgctcgcc tggcgcacga gcccggagac cacgagctcc gtaccactga
    11521 tggatgatcg ggtcaccagc gcgggaacgg tgcccactcg accgtgcagc gagaactgca
    11581 cgccagcgtc ggtcgtggcc gggccgtacg tgtcgaggcc gcaggtggcc agcagacccc
    11641 caccgaacga tcgcagccac gagtcaccgg tgcactccgc gatcgccggg gcgaccattc
    11701 cggtcggcga catccaggcg atcggcgtgc cgcgataggt cacctgcccg aggtcgagcg
    11761 cacggtcggg atggatctcc acctcgagcc cgcccccggt gacgaggcgg atgcgacggg
    11821 caccccgcgc gggtccgtca tcctccacga ggcggtcgat ccgtgccacc tgggcgagga
    11881 tgccggttcg tcgtgccacc tcatcgagag ggcgaccgtg gagtgtcggt ggcgggttgg
    11941 tcacgcgcgc tccgctgcga cggcgcccct gtcggcagcg agctcccggt tgccaggacc
    12001 gaagtgcttg agcagcacga ggtcggcgct cggcgaggtg ttgcgaatgc gcacccccgc
    12061 ttgggcagcg gacgaggtca cgaagtactc gtcgtgcgag agctgtccgt atcggatcga
    12121 cgagatcgcc gagacttcct ggccattgat ctcgccgaac ccgtccacgg cgatgaagcc
    12181 gtacgcatcg gactcgtcga tggagacctc gcagcccggt gccacggtga gctccttggc
    12241 gctgaacgca ggcgaccggt aggtgatcca gcgctcgaca taccgatcgc ggccggccgc
    12301 ggcgctggcg tccgtctcca cgggcggcgt gaaacggtga ctccagaact cgggatcgac
    12361 gttgaggtcc cagtcgatga tctcgaggat gaactccagg tcacctaccc gatcggcggg
    12421 cacgtccttc cagagcagct catccgcaac ctcgcgattg ttcgaccacg attcggtcat
    12481 ggcgaagacg tcgctggccg cctgcggctc gtaggtgcac atgctcgcag gggcatgcag
    12541 gactcccggc ggcacatccc atcccgtgcc cggagtgatg cgatgcgcgc tcgacaactc
    12601 ggtgatgcgg ttgtcaccgc cgcgcaggaa cgcttcgagc ttgtcaagaa cctgctgtcg
    12661 ggtcgtctcg gggtggaggc cgaagaaggt gtacgggaat tcgccgccgt ggttgttgag
    12721 ctgcggcggg aagtagtagg cctcgggctt gccccgcttg ccgacaagtg ccgcgtgctc
    12781 gtccttctgg tgcaagtgga acgggagtgg gaactggttg tcgaagaact tcgagtacat
    12841 cggccaggtg ccgtactccc cccagagcgg gccgatcgcc tccgccttga ggtgcgcgat
    12901 gaactcatcg aatgggatga cctcaccgtc ggccccgacc acgagactga gcccctcata
    12961 cgggtcggtg agcggaccgt tctcggcccg gatcgcgctg gcgaaccagc gctcgtcgat
    13021 tcctccccgc tccttgccca gcggaaagta gtcgtcgggg tggagccgga gacgtcgacc
    13081 cggggtgcag aacgaccgcg gcacccaggc cggagccagt cgcagcacgc caccgcccgc
    13141 ctccatagcg cgttcgatgc tgccctcggc tgaggtcacg ccgtcgtagg gtcccgcctt
    13201 ggtcatggtg ccatcccatc ggttcaatga accattgatc gttccagcga agttactggc
    13261 acgccgtcgc ccgtgtcaag cagtgcgagg atggccccgc ccgcggcgtg agtacgctgt
    13321 gctcatgtct ccactgctcg gcaccgaacg ccgcgagctg ttgctgtcga tactcgggcg
    13381 cgccggcgtc atccgcctcg acacagccgc cgaggagctc ggggtgtcgg tgatgacggt
    13441 acgtcgggac ctcacggatc tggaggggga ggggctggtt cgacgcgtgc gcggcggcgc
    13501 ggtcgcgccg atcctgcccg agccctttag cgcccgagcc gcctcgcggt cggcagcgaa
    13561 gagcgagatc gctcgcaagg cgctggcgct ggttccgtct cggggcgcgg tcgccttcga
    13621 cgcctcaacg accagcggcg ccgtgctggc cggcgtcgac ggcggtgacc tgatggtctc
    13681 aacgaattcg gtcgagaatg ccgccgcggc acggcgccgg ccgggcgttc gctccgtgct
    13741 ggtgggcggc gagctggagc agcgcaccgg cagcttcgtc ggaccgatcg cccacctggg
    13801 ggcccgcagc ctgagctacg cggtgttctt cagctctgcg tcggcggtcg acgccgagtt
    13861 cgggccgagc gaggcatccc tggcagaggt tgaggtcaag cggtgcttcg ccgaacaggc
    13921 cgaccgcacg gtactgctcg tggatgcctc gaagctgggc ctgcgcgcgc tcgctcgcgg
    13981 cctggactgg cagtccatcg acctgctcat caccgagctg cccgcagcgg atgagcgcct
    14041 cgatccctac cgtgaccttg tcgagttgcg atgatgctgt tagtttttgt taaaccgctc
    14101 aatacctgac aggacttccg atggcgacca cgcccagcga cctcatcgcc cgctccaacc
    14161 gcctgggttc cgacccgcgg gtaaccaact acgccggcgg taacacgtcg gcgaagggaa
    14221 ccgagatcga cccggtcacc ggtgagcccg tcgaactgat gtgggtgaag ggctcgggcg
    14281 gcgacctcgg cacgctcacg gagtccggcc tcgccgtgct ccggctcgac cgcctgcgcg
    14341 cactcgtcga cgtctacccc ggactcgacc gcgaggacga gatggtcgcg gccttcgact
    14401 acacgctgca cggcaagggc ggcgccgcgc cgtcgatcga caccgccatg cacgggctcg
    14461 tggatgccgc gcacgtcgac cacctgcacc ccgactccgg catcgccttc gcgacggcga
    14521 aggacggcga gaagctcacc aagaaggcgt tcggcgacaa ggtcgcgtgg gtggactggc
    14581 gtcgccccgg cttccagctc ggactcgaca tcgccgccat caaggcggcc aacccgcagg
    14641 ctgtcggcgt gatcctcggc ggtcatggca tcacggcgtg gggcgagaca tcgaaggagg
    14701 cggagcagaa cagcctctgg atcatccgga cggccgagga gtacatcgcg gccaagggca
    14761 ggaagaaccc gttcggcgcg accgtcaaga agaacgtggc cctcccggtg gccgagcgtc
    14821 gggccaaggc ggccgcgctc gcggcgacga tccgtggaat cgccagccac gaccggccca
    14881 tggtcggcca cttcaccgac tccgacgtgg tgctcgactt tctcgcgtcg gcgagcgccc
    14941 cccggctcgc ggccctgggc acgagttgcc ccgaccactt cctgcggacg aaggtgaagc
    15001 cgctgctgct ggatctgccg gcgaatgcgt cggtcgaggc atccatcgct cggttgaagg
    15061 agctgcacga ggcgtaccgg gccgactaca cggcgtacta cgagaagtac gctgaggtct
    15121 cgactagctc gaccaccgga agcccgccca tgcgcggcgc cgacccgctc atcgtgctcg
    15181 tgcccggggt gggcatgttc agctacggcg ccaacaagca gaccgcgcgg gttgccggcg
    15241 agttctacgt caacgcgatc aacgtcatga ggggcgccga ggccatctcg tcatatgcgc
    15301 cgatcagtga cgcggagaag ttccgcatcg agtactgggc gctcgaggag gccaagctgg
    15361 ctcgcatgcc gaaacccaag tcgcacgcga ctcgtgtcgc gctggtgacg ggcgcggcat
    15421 caggaatcgg caaggccatc gcgaccaggc tcgcagccga cggcgcctgc gtgatcatcg
    15481 ccgacctcga cctcgccaag gcacaggctg cggcggccga gatcggtgac accgatgtgg
    15541 cgatcggcct ccaggtgaat gtgacggatg ccgcggcgat cgccacgatg gtgcaggatg
    15601 ccgtgctcgc cttcggtggc ctcgacatcg tcgtcaacaa cgctggtgtc tcgctctcga
    15661 agccgctgct ggagaccacc gagcaggact gggacttcca gcacgacatc atggccaagg
    15721 gatcgttcct cgtatcgaag gcgacggcac cggtgctgat cgagcagggc atgggcggcg
    15781 acgtgatcta catctcgagc aagaactcgg tgttcgcagg ccccaacaac atcgcctact
    15841 cggcaacgaa ggccgaccag gcccaccagg tgcgactgct cgccgtcgag ctcggcgagc
    15901 acggcatcaa ggtcaacggc atcaaccccg acggtgtcgt gcgcggctcc ggcatcttcg
    15961 ccgcgggctg gggcgccaac cgcgccaaga cctacggcat cgacgagcag gacctcggca
    16021 agttctacgc ccagcgcacc atcctcaagc gtgaggtgct gcccgagaac gtcgccaacg
    16081 cggtatcggt gctcaccggc cccgacctca gccacacgac cggactgcac atccccgtcg
    16141 acgctggcgt cgctgcggcg ttcctgcgat gaacggatct cgacggcctc gatcaccggg
    16201 cacggtcgcc gcggtcgacc tgggcgccac gagcggtcgg gtgatgctcg gccacgtcga
    16261 ccacaacgag ttgagcgtgc ggccggtggc tcgattcccc aacggccccg tgcagcgcgc
    16321 cgatgggctg cactgggaca tcgacgccct gtacagccac gcccttgccg ggttggctgc
    16381 agcgctgcgg gaggaaccca cactcgccgg gatcggtatc gactcctggg ctgtcgacta
    16441 caccctcctc acgggcggcc ggatgctcgg actccccttc cactaccgtg acgagcgcac
    16501 cgcccgcggc gtcgagcggg tgcactcagt ggtcagcccc gaggagctct acgccgccaa
    16561 cggcctgcag ttcctgccct tcaacacgct ctaccagttc gccgccgagg gcaacctcga
    16621 cacggtcgac tccttcctgc tgatcccgga tctgctcgcc tactggctca caggccggcg
    16681 cgtcgcggag cgcaccaacg cgtcgaccac cgggctgctg ggggtcgcga ccgggcagtg
    16741 ggatgacgcg ctcatcgagc gcctcgcact gccgcgccgc atcttccccg agctggtgga
    16801 cccgggcacc ccgctgggct cgctgcgtcc cgaggctgcc gcgatcgtcg ggggtgacct
    16861 gagcgtgacg gcggtcggat cgcacgacac ggcgtcggcg gtcgtggccg tgccggcggc
    16921 atccgatgac ttcgcctaca tctcgtgcgg aacctggggc ctggtgggcg tcgaactgga
    16981 gacgccggtg ctgacggagg cgagccggct cgccaacttc accaacgagg gtggcgtcga
    17041 cgggcgggtg cgctacctgc acaacgtgat gggtctgtgg ctgctgagcg agtcggtgcg
    17101 gcagttcaac gacgagggca agcgcgacct cgacctgccc gagctgctgg ccgcggcatc
    17161 cgcgctgacg acgccggtga gcgtgttcga cgcgaacgac gcctcgttcc tgccgcccgg
    17221 tgacatgccg gcgcggatcg ccgcgtggtg cacgacccac ggtctcgccg caccgcagac
    17281 cccggtcgag ttcgtgcgca gcatcatcga gagccttgcc gtggcattcg cagacgcggt
    17341 gcgcacggcc tccgagctct cgggcaagac ggtgtccgtc atccacatcg tgggcggtgg
    17401 atcacagaac gagctgctct gccagctcac cgccgatcgc agcgggcttc ccgtgctggc
    17461 gggaccggtc gaggcgaccg caatcggcaa cgtgctcgtg caggcccgcg ccctgggctt
    17521 cgcgagcggg tcgctcgagt cgctgagatc gctcgtcgcc ggcgcattcc tgcccgtgct
    17581 gtaccgaccg cgctagcggg cgtcccgcca ggagtgcttc ggcttccagc cgaggatgcg
    17641 ccgcgccttg gctgtgctcg tgagcgagtc gttgacgccg agctcgccgc gcacctcgac
    17701 cccggggaag aactcggcca tcagctcggc gttcgggcgc tcgagcaccg tgtcgcccgc
    17761 gaagatgacg aacacgtcgt atcccggagt cccggcctcg agtgcgagac ggatcgcctc
    17821 ggcgccgtcc cgcgcgtcga tgtagcccca caggttccac ttgcggctct cggcggtaaa
    17881 cgacgcgaac tcggcgtagt cagcgtcgac catcacgttg gagaagcgca gtgcggtgat
    17941 gctcagctcg ggatcccagc gggcgatctt gcgcgccaac tcctcctcga ggtgcttgcc
    18001 cagcgagtag acgctctccg gccgggtgtc gtactcctcg tcgaggggaa cgtagggcgg
    18061 cggggcgtcg aagggcagtc cgagcaccgt ctcgctggag gcgaaggcga tgcgacggat
    18121 accgaggcgc cgggcggcct ggaacacgtt gaaggtgccg acgacgttgt tgtggaaggt
    18181 cgcggcatcc gtgagtatgc cgggcgcggg gatcgcggcg aggtgggcga tcgcatccac
    18241 gccaccgtgc cggtcgttga ttccggccag cgcgtcgatc acctgcccgt agtcggtgat
    18301 gtcgagctgg gtgaaggccg agcgatcggg gcctgcctga tcgagcacga ccacctcgtg
    18361 gcccgcggcg cgcagaacct ccacggtgac cctgccgagc ttgccgtttc cacccgtgac
    18421 cgcgattctc atgggtccat tctcctcctt cgcgaatgtc tgacaatctg ctacattgtt
    18481 agacattctg gagccaaggg aggcagcgtg acggtggaac cgacgcccgt gctgcccgac
    18541 accatcgccg atgagctgcg ggcgagcatc ctgagccagg tggatgcccc gggctcggcc
    18601 ctcacggagt ccgccgtcgc cctgcgcttc ggggtcgctc gacccaccgc ccggctcgcg
    18661 atcgacaaac tcgtggccga tggactgctg cggcgcgagg cccaccgcgc cgcacgggtg
    18721 cccgaactca gccgcgacga catcgtggac ctgttcgaca accgcgccat catcgagtcg
    18781 gcagcgatgg ccgcgctcgc taccgcgggc accatcccgg ccgcggcagt cgccgcccac
    18841 cgcgctctca ccaccaccga ggacttcgcc cagcacgaca tcgccttcca ccgcgctctg
    18901 gtcgcgggcc agccgagccc gaggctcacc cgcctgcacg agctgctcat gggcgagatc
    18961 gagctgtgca tcggacaggt gcaggccgcg cacctgctca gctccgccga gatcgcccgc
    19021 cagcaccagg gcatcctcga tgccatcacc tcgggcgacg cggatgccgc ggcgcgcctc
    19081 acccgtgacc acatcgtcgg ggcgcgcgac cgcctcctcg cacgaacaac gaagggcccg
    19141 cagtgaccaa gcgccagttc ccgaaccccg tcgagctgct caagctgatg aagttcaaga
    19201 agccgacctt caacgcgaag aagcgtcgcc tctccggcgc gctgaccatc gacgacctgc
    19261 gcctgatcgc caagcgccgc acccccaagg ccgccttcga ctacacggat ggcgccgccg
    19321 agggcgagct gtcgatgtca cgctcgcggc aggcgttcga ggatgtcgtc ttccacccgt
    19381 cgatcctgcg cgacgtgtcg acagtggaca cgtccacgac gattctcggc ggaacctcgg
    19441 cgatgccgtt cggcatcgcg cccaccggct tcacccgcct catgcagacg gagggggaga
    19501 tcgcgggggc cggcgctgcg ggcgcggcgg gcatcccgtt caccctgtcg acgctcggca
    19561 ccacctcgat cgaagacgtg aaggccgcca accccgacgg ccgcaactgg ttccagctgt
    19621 acgtgatgcg tcagcgtgag atctcctacg gtctcgtcga gcgggcagcc gccgccggat
    19681 tcgacaccct gatgttcacc gtcgacaccc ccgttgcggg cgcacgtctg cgcgacaagc
    19741 gcaatggatt ctcgatcccg ccccagttga cgctcggcac gatcgtcaac gcgatccccc
    19801 gcccgtggtg gtggtgggac ttcctgacca ctccgccgct ggagttcgca tcgctgtcgt
    19861 ccaccggggg cacggtgggc gagctgatcg actcggcgat ggatccctcc atcagcttcg
    19921 aggacctgaa gatcatccgc ggcatgtggc ccggcaagct cgtggtcaag ggcgttcaga
    19981 acgtcgagga ctcgaagacc ctcgccgacc tcggtgtcga cggcatcctg ctctccaacc
    20041 acggcggccg tcagctcgat cgcgccccga tccccttcca cctgcttccg gaggtcgtgc
    20101 gcgaggtggg caaggacacc accgtgatgg tcgacaccgg catcatgaac ggtgcggatg
    20161 tcgtcgcatc gctcgcgctc ggcgccaagt tcactctcgt cgggcgcgcc tacctgtacg
    20221 ggctcatggc gggcgggcgg gccggggtcg accgcacgat ccagatcctg tcggagcagg
    20281 tggtgcgcac gatgaagctt ctcgaggtgg cgaacgtcga ggagctcggc ccgcagcacg
    20341 tcacccagct cgagcgtctc gtgccccgtg ccgtctcgcc ggcgttgacc tcggccgtgc
    20401 ccgcgagcaa gcgaacggtg gtgcgcaccc ccgccgcgaa gccgaccgca gcgaaggctc
    20461 cggcgcgtcg gtcctgaact cccgcggccg ttcgtggtgg gcggccgcgg gtcctcccct
    20521 agggtgaggc tatgtcgtgg gtctatcccg tctcggaggc cgcacgaacg acccggctcg
    20581 gcgtggtcgc ggcctttgcc tggaccggcg tagccctgct cgccgccggt gccgccgcga
    20641 cgctcgtggt cggtctctcg gagtccggag tggtgccgtg gctcgcgctc ggcggattcg
    20701 tgctggccgc cgggaacctg atcgtctccc gccgcttcga atccggcggc cctgtgccgc
    20761 tcgccgcggg ggcggcatcg atactggcct gctggatggt ggccgcgggc atccgactca
    20821 ccgacctgcc gttcgacggc tacgtggggt tcccgctctt cgcggtcggc gtggctgcaa
    20881 ctctggttgg cgccatcgcc gatcgttggt cgggggccat cggcaccctc atcgggttcg
    20941 tcgcggggca ggcggccctc gtgctcgcgt atcagggtgc cgtgccagcc gggctcgtga
    21001 tcgatcccgc cacagccgct gtcgccgtca tcgtcgccct gttcatctcg atgctcagcg
    21061 tcgtgcgccg ccgggcccgg gcgagcgggc cacgactcga cgactccggt cgacgagatc
    21121 tgacggcgca cgagcgccag tcccttctgt tgaattcccg ggccctcgtg cacgacaccg
    21181 tgctcggtga gctggcggcg ctcgggatga cgcggccggg tcctctgacc cagagcatgg
    21241 ccacgagcat ccgggccagt ctcgtggcgg cggcctccgc cgccgggccc tcgccggagc
    21301 ccggaccgcc cccggtcgcg ctcgcggatc tgctcacccg attccgcatc gcggggctgc
    21361 acatcaccgt gaccggtgac ccgagcgcgc tcgaccgcct gtcggctgat cggcgggcga
    21421 gcctcgtgct cgccatggaa cagtgcctcg tcaacgtgct gcagcactcg ggcgtcactc
    21481 acgccgagtt gtcggtggcg gtgagcgagg gacgtctgat cacgacggtc gtcgacgagg
    21541 gcagcggctt cgacgagggc gcggtaccgg ccgaccgctt cgggttgcgc gaatcgatcc
    21601 gggggcgcat agccgacctc ggcggagcgg tgcgggtgct gaccggtgcg ggcacgggaa
    21661 catccgtgat tgtcagcgtg cccatcgacg gagactcgac gtgagcgcgc gggcgaccgg
    21721 gcgccaggcg agccagcagc gcgcaacacc gcagcgactg gatcccctgg gcgggctgac
    21781 agcgcagtac ttcatctgga gcgtggcgat catcgccgtc gcggtcgccg tcacccaggt
    21841 cgtgcgacac gcgaacgatg tgcgctcgcc cgctctggag gcgatcgcgc tgctgtgcat
    21901 tgtcgccgcc gggggtgcgg tggttgacgg tgccgcggcg cgacggttcc ccttctcggc
    21961 gcagcgccac gcggtcctcc acgtgctcgg ggtgcttgcc gtcgcgttcg atctcgcggg
    22021 ccgcgaccac ggtgccggcg agtctgcggc ctggggcgcg gtctgcctcg cgatcctcct
    22081 gatgaccatc ggctcctacc ggccggcggc cgagatcctg ctgatgacgg cagtcagctc
    22141 gatcaccgtg gccgcgctcg tcgtcgggtt ctccgccggc ctcacaccgg cgcaggtcgc
    22201 cctgttcgcg attacccgcg cagcgccgat cgccgcggtc ggggcggggg cggcggcgtt
    22261 ttcgaacact ctcgtcacga ggctcacgca gtggcagaac ggcgcggccg agcgtgcgga
    22321 gatcgcgcgc gcgaccgtgc gggaggagct gctcgcggag gtgtcgaacg cgaggcacga
    22381 gctcctcgag caccgcgcgg cacccttcct gcaggatgtt ctcgatcgcg gagtggtaac
    22441 ccccgccgac gcagctcggg cccgcgggct ggccaccgcg ctgcgacgca cgatgctggc
    22501 cgaggccgga cggggctggc tcgtcgaact cgccgacacg gtcgatgacc ccgagaaggt
    22561 c
//
LOCUS       DAOSOU010000007        21139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2504_length_21139_cov_6.670556, whole genome shotgun sequence.
ACCESSION   DAOSOU010000007 DAOSOU010000000
VERSION     DAOSOU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 21139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21139
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2504_length_21139_cov_6.670556"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLW15_00810"
     CDS             <1..306
                     /locus_tag="PLW15_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32970.1"
                     /translation="ELEFVLSGGGPFGAWLYEKFTNPPTAIRIGDHWPQGDSPVVLTT
                     ALGTESSSTRRLPVPWIADDLSYGAHPHPREMILYDRVDTRLLFSDFAARISLLGRG"
     gene            339..1139
                     /locus_tag="PLW15_00815"
     CDS             339..1139
                     /locus_tag="PLW15_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT32971.1"
                     /translation="MTAPAESETAFRPSWWILALVGVIGGVLSGAFGVGGGILMVPLL
                     IGLAKMNQRQAAATSLLAIVPTAIAGSATYLIASQVDVFAALFLGIGGVVGSLYGTRL
                     LKQLPLGVLRWGFIALLVVVAIRMVLVIPDRSSGHLEPSVLAALGLVAMGLFVGVASG
                     LFGIGGGVIAVPALIAVFGLGDLIAKGTSLLFMIPTAVTGSVNNTRNRLVDVRAGLIV
                     GVAATVASFGGAALAFVMSPAVSSYLFAALLLVSAVQLTVRAIRMRRA"
     gene            complement(1148..1480)
                     /locus_tag="PLW15_00820"
     CDS             complement(1148..1480)
                     /locus_tag="PLW15_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32972.1"
                     /translation="MSVDAGFVAVLTDLLESTDVAHRAYEEAHGPAPEWPQWYAARMV
                     RALGDSYREQDILSALLSAATAHGVHEQVTGRPDPEWASWYAEHMAARLSRDWYASAA
                     EEEAIWDA"
     gene            complement(1477..1920)
                     /locus_tag="PLW15_00825"
     CDS             complement(1477..1920)
                     /locus_tag="PLW15_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT32973.1"
                     /translation="MEMKLEVLTVPVTDVDRAKEFYERLGFRLDIDFVGEDGFRVVQF
                     TPPGSQASIIIGSGITDAGPGSVENLHLVVSDIVHARTELAGRGIDVSEVWHDAGGIF
                     HHSGGRARAEGPAPDRADYGSFVSFSDPDGNGWVVQEIVNRAPGR"
     gene            complement(1995..2516)
                     /locus_tag="PLW15_00830"
     CDS             complement(1995..2516)
                     /locus_tag="PLW15_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT32974.1"
                     /translation="MAALSRSRRSASGIALIVSGALFLLAVVLPLAGVAVPWLAALAY
                     AALAVGLVILALGAVNNTIAKVSLLVGAVGFAILALAGLGLGLPGVLTTIGAVIAALG
                     FLVGAIVLYVGKEITNTAAIIFVVAAILAAVILLGVAAALGLGAIATILTVALGIALV
                     VAGVYFRRAENRR"
     gene            2667..4124
                     /locus_tag="PLW15_00835"
     CDS             2667..4124
                     /locus_tag="PLW15_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT32975.1"
                     /translation="MSTSDLGHDFRPGKIIAMHLNYPSRIAQRGRVPAHPSYFLKPAT
                     SVAATGGTIERPAGTELLAFEGEIALIIGRPTRRVSPEEGWDAVSGITAANDFGVYDL
                     RYVDKGSNFRSKGGDGFTPLGPSIIDARSVDPAKLRVRTWVNGTVAQDDTTADLTFSL
                     GLLVADVSQLITLEPGDVILTGTPAGSSVVVPGDVVEVEVTTADGALSSGRLVTTVTQ
                     GVHELPAFSAGPKVDDLQREEAWGSRAAAGLPEEASILTEHLKAKINSVATATLSSLL
                     RKRGFNNVSIDGLIATRPGARLVGLARTLRYIPNREDLFDRFGGGYNAQKRLFDSLRP
                     DDVVVIEARGDSGSGTLGDILAIRAKHLGAAGIVTDGGVRDLAVVTELGIPTYHAGGH
                     PAVLGRKHVPWEYDTTIGCGGAAVQPGDVIVGDDDGVLVIPPSLIADLVDEAIAQEKE
                     EAFIAAHVAAGEPVEKLFPLSPEWREKYRASVGES"
     gene            4121..4786
                     /locus_tag="PLW15_00840"
     CDS             4121..4786
                     /locus_tag="PLW15_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT32976.1"
                     /translation="MTASVGINKSERAYLEIKSKITDGSFGPGYRLVLGQIANELQVS
                     VVPVREAIRRLEAEGLVTFTANVGAQVVATDAREYQYTMETLSLVEGMATAMAAPNVT
                     PGDLVLARSINVEMRECLEHFDPKRFTELNHEFHAVIFEHCSNLHILDLVHRGWNRLA
                     TIRESTFAFVPGRAQRSVEEHDHILDLIEAKAPALEIEIAARSHRLATLHEYLEYQKS
                     QQS"
     gene            4826..6325
                     /gene="hpaE"
                     /locus_tag="PLW15_00845"
     CDS             4826..6325
                     /gene="hpaE"
                     /locus_tag="PLW15_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HOT32977.1"
                     /translation="MTQHHVPEDLPTHLQHFIDGQWVDSIGGETFDVLDPVSNQNYAT
                     CAAGQKADIDRAVAAAKQAFDSGPWPRLKPRDRFRILNRIADLVEERDARLAELETFD
                     TGLPITQALGQAQRAAENFRFFADLIVAQADDAYKVPGTQLNYVNRKPVGVAGLITPW
                     NTPFMLESWKLAPALASGCTVVLKPAEFTPLSASLWAEIFREAGVPDGVFNLVNGIGE
                     EAGASLVAHPDVPLISFTGETTTGKIIYRSCADNLKGMSMELGGKSPAVVFADADLDA
                     AIDSTLFGVFSLNGERCTAGSRILVERSIYDEFCTRYAERAKNIVVGDPHDPKTEVGA
                     LVHPEHYAKVMSYVEVGKTEGRLLAGGSRPEGLDEGNYVSPTVFADVAPTARIFQEEI
                     FGPVAAITPFDTDEEALALANGVRYGLAGYIWTNDLTRAHTFAQNVEAGMVWLNSHNV
                     RDLRTPFGGVKASGLGHEGGYRSIDFYTDQQAVHITLGPVHTARFGATR"
     gene            6353..7426
                     /gene="hpaD"
                     /locus_tag="PLW15_00850"
     CDS             6353..7426
                     /gene="hpaD"
                     /locus_tag="PLW15_00850"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HOT32978.1"
                     /translation="MSSPIPVPTSPAPDILRCAYMELVVTDLARSREFYVDVLGLVVT
                     EEDDEAIYLRSFEEFIHHNLVLRKGPVAAVAAFSFRVRSPEDVDAAEAFYTELGCPTD
                     RRAGGFVKGIGDSLRVVDPLGFPYEFFYETTHVERLAWRYDLQSPGALVRLDHFNQVT
                     PEVPRAVGFMESLGFRVTEDIRDDQGTVYAAWIRRKPTVHDTAMTGGDGPRMHHVAFA
                     THEKHNIIAICDKLGALRLSDHIERGPGRHGVSNAFYLYLRDPDGHRVEIYTQDYYTG
                     DPDNPLVSWDVHDNQRRDWWGNAVVPSWYTDASLVLDLEGNVQPVVKRAEPSELEETI
                     GADGFSYTRKGETTEGFKLGHQL"
     gene            7458..8243
                     /gene="hpaH"
                     /locus_tag="PLW15_00855"
     CDS             7458..8243
                     /gene="hpaH"
                     /locus_tag="PLW15_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HOT32979.1"
                     /translation="MLDDATLTAIADELLEAQRSGVPIPLITARHPGMTVEDSYAIQR
                     IWADRKRAGGSRLVGRKIGLTSKVMQVATGISEPDYGVIFDDMVYQTGARIEIDQYIN
                     TRIEVELAFVLGRPLSGPNCTITDVLDATAYVIPALEILSSNIEMEGRTIVDTISDNA
                     AMGAMVVGGTPTKPSDVDLRWVAALLYRNETIEETGVAAAVLGHPAAGVHWLANKIYA
                     HGDSMVAGEIILAGSFTRPMWVYKGDTVHADYGPLGAITCQFV"
     gene            8228..9037
                     /locus_tag="PLW15_00860"
     CDS             8228..9037
                     /locus_tag="PLW15_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HOT32980.1"
                     /translation="MSVRLEPPLSFASRLRDSTRTLVGVWVCSGSPTVAEICAGSGVD
                     WVLIDSEHAPNGLESILAQLHAVQGYPATPVVRTPHGDPNLIKQYLDLGVQNLLVPMV
                     NTAADAEQLVRAVRYPPLGMRGVGSALARASRWNRVDNYLHRAGEFISLFVQVETGAA
                     VGNIDEILSVDGIDGVFVGPSDLAASMGLLGQQDHPDVVAAVEHVIARARQAGRPVGV
                     NAFAPALAERYIDGGIDFILVAADVSLLARSTEEIAARFIADTSDERRTSY"
     gene            9289..9624
                     /locus_tag="PLW15_00865"
     CDS             9289..9624
                     /locus_tag="PLW15_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOT32981.1"
                     /translation="MKYMMFVATDPEAEPYDPAQDNIQEWVAEMVARGVNLHGDRLRP
                     IEDATLVRVRNGEVLVTDGPFTESKEWIAGYDMLEAADLDEAIEIASKHPMARFGRIE
                     LRPVWPFEE"
     gene            9681..10841
                     /locus_tag="PLW15_00870"
     CDS             9681..10841
                     /locus_tag="PLW15_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT32982.1"
                     /translation="MSTLIRVTGDWSLAEDCTAEAFEAALATWPRDGIPTNPGAWLTT
                     VAKNRALDRLRRAATLARKLEEMTAVTELEHLDPELSDFPDDRLRLIFTCCHPALSLE
                     ARVALTLRTVGGLTTAQIARAFLVSEATIAQRIVRAKRKITEAAIPYRVPEAHLLAER
                     LSGVLAVLYLAFNEGYSVVAHTSIADEAIRVTRALAQLMPGEPEVTGLLALMLLQHSR
                     RRARLDAEAVPLTLEEQDRSLWDAAAIAEAGELLNGAGRGAEPGPYRLQAAIAAVHAT
                     ADSAAATDWQTIVALYDRLLTVTPSPIVELNRAVAVGMASGPEVGLAALERVAASGAL
                     DGYYLLPAARADLFRRLGDYSKAAAFYRVALAGAPTQPERDFFARRLDEVGG"
     gene            complement(10838..13195)
                     /locus_tag="PLW15_00875"
     CDS             complement(10838..13195)
                     /locus_tag="PLW15_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT32983.1"
                     /translation="MSATPHPADSHDLIRVQGARENNLRNVTVDIPKRRLTLFTGVSG
                     SGKSSLVFGTIAAESQRMINETYSAFVQGFMPSQSRPDVDVLEGLTTAIIVDQERMGA
                     NIRSTVGTATDANAMLRILFSRLGQPHIGSPNAFSFNIPTSQTSGVRTSSTGETTVIE
                     NETYLGGMCPRCEGTGNVTDIDLTQLFDDTKSLEQGALTIPGYTVDGWYVRIFTASGF
                     MDPAKPIREYTEQERADFLYKEPVKVRIDNINMTYEGLIPKVTKSMLSKDVDAMQPHI
                     RAFVERAVTFTACPECGGTRLNAGARSSKIGGLNIADACALQISDLADWVRSLDEPTV
                     APLLATLQQILDSFVMIGLGYLSLDRPSGTLSGGESQRTKMIRHLGSSLTDVTYVFDE
                     PTVGLHPHDIQRMNELLLALRDKGNTVLVVEHKPETIEIADHVVDLGPGAGTAGGELV
                     YEGTVAGLRASGTLTGRHLDDRASLKTSVRASTGTMPVRGATSHNLQGVDVDIPLGVL
                     VVVTGVAGSGKSSLIHGSVSKREGVVAVDQSAIRGSRRSNPATYTGLLDPIRTAFAKA
                     NGVKPALFSANSEGACGNCNGAGMIFTDLGVMASVSSVCEVCEGRRFDASVLGYKLGG
                     RDISEVLAMPVTEAEEFFGAGDAKLPAAHAILARLADVGLGYLSLGQPLTTLSGGERQ
                     RLKLATQMADKGDVYVLDEPTAGLHLADVENLLGLLDRLVDSGKSVIVIEHHQAVMAH
                     GDWIIDLGPGAGHDGGRIVFEGTPADLVAARSTLTGQHLATYVGA"
     gene            complement(13268..13714)
                     /locus_tag="PLW15_00880"
     CDS             complement(13268..13714)
                     /locus_tag="PLW15_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT32984.1"
                     /translation="MPAASLSLLDPTNEVHARALARLGEERIAWFTTIGRDGFPHAVP
                     VWFIVHDDSVLVLSEPATAKVRNLRANDHALVHLEAGTDGEHLTVLQGRATISDRPAT
                     EWIDEIGAVYEAKYRGGLAGISLTLETMAAKYSSVIQLRPEKLIAW"
     gene            13839..14360
                     /locus_tag="PLW15_00885"
     CDS             13839..14360
                     /locus_tag="PLW15_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT32985.1"
                     /translation="MRTSSSAGFDLWEAVDGLREAFAPRVSGRMGRGDIRTAILVALT
                     EEPMHGYQIIQSIEKSTRGAWKPSPGSIYPTLQLLADEGLVTSEQVGERKVYSLTDAG
                     KEAAAEFSSGPEPWEPRRSWRTEHNVAVPKAGAKLAQAIAQVAQGTPEQTERAVEVID
                     EARRKIYAILAED"
     gene            14365..16065
                     /locus_tag="PLW15_00890"
     CDS             14365..16065
                     /locus_tag="PLW15_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOT32986.1"
                     /translation="MPPASGPLTAPPTGRPFRARYRRIMRFASRALAQAWWFELVLPR
                     IGLGRLAARGRVRRLEKLANRFYVLAADLGGLMIKVGQFLSSRLDILPPEITKALEGL
                     QDEVAPESFERIRVQTENELGMPLELAYASFEPVPIAAASLGQAHRARLSPAIAADLG
                     FEEVVVKVQRPGIEAIVAVDLAALRRVARWLSRVRLVSSRADAPALVEEFAATSLEEI
                     DYLHEAGNAERFAADFADDPSVGAPVVVWDRTALRVLTLSDVTAIKITDVASLTAAGI
                     DPAEVAQALARATFQQIFVAGFFHADPHPGNIFVTPTPGEGDGWRLTFIDFGMMGEIS
                     DSLRSGLREFILAAVARDGRSLISAMENLGVLLKSADTEELERAMTTLFDRFGGMGVA
                     ELQRVDPRELERFALQFGETIRALPFQLPESFLLLIRTISLISGVTSALNRDFNMWDA
                     VDPFARTVLNNSGTGTIRELAQQALGYANTLARLPKRLDELTARIDRGQVAVRTPDLD
                     RRLRTIERSVGRVASAIVFAALLFAGVLLRPSDPVLSWVLMGASALPLLHVLVTWRGR
                     "
     gene            16062..16892
                     /locus_tag="PLW15_00895"
     CDS             16062..16892
                     /locus_tag="PLW15_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOT32987.1"
                     /translation="MTRARANVPARAAAAVGLAFALMLTGCTSPPSAPDPAPDGAPFP
                     VDATADYQLGGAYPPPAGVTLVARDSTEEPLAGAYSICYINGFQTQPGDQWPDDLVLH
                     DGQQRLVDPNWPDENVIDISTAEKRAAAASRIEQAIATCAASGFDAVEFDNLDSYTRS
                     DDHLTLDDAIAFASLLVDRAHELGLSAGQKNTSELGQVGPDTIGFDFAVVEECDQFEE
                     CGAYTDVYGGRVIDIEYTDALRRPFVEVCADAATPTITILRDRGLAPQGDPAHHYESC
                     "
     gene            complement(16942..17598)
                     /locus_tag="PLW15_00900"
     CDS             complement(16942..17598)
                     /locus_tag="PLW15_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT32988.1"
                     /translation="MEKHERAADAGSQTLSRGLAALALIGESRIPLTINDLAAALGVN
                     RSAAYRLVRTLERHRFVQRSLSGDLTLGVRLAAFGQSVSRDLQAAALPELQRVADDLQ
                     MTAFLVVFDGEEAVTLLSVEPRNAATFVAQRPGRRHPIGRGSPGRVIQSIVDPAHHPP
                     ARYELSHDEVIPGLSAIAVPLRSGRAASIAVLYATRPVEVELIVDRLEAASALISATG
                     "
     gene            17739..>19489
                     /locus_tag="PLW15_00905"
     CDS             17739..>19489
                     /locus_tag="PLW15_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOT32989.1"
                     /translation="MQIHLRGYEATDPRIKPAAGFGIDRPAELPDEMDVLIVGTGPAA
                     VVVAAQLSRFPSINVRAIEQRAGRLEVGQADGIQARSVETFQAFGFATEVVDEAYRNV
                     EMCFWKPDPDDPSVIVRASRAADDATGMSEFPHIYVNQSRILDYFLRDAARAPGRLVP
                     DYGIEFVDCTIDTEAEYPVSAQVRYVAGDREGEIRTVRAKYLVGGDGARSRVRTALGH
                     RLHGEASMHAWGVMDILANTDFPDIQVKCSIQSHEAGNILTIPREGGYLVRVYVDLGE
                     VGEDDDGAIRATPIEAIIAKANRIIHPYSLDVRAVTWWSVYEVAHRITDGFDDVPPEL
                     VGTRQPRAFIMGDACHTHSAKAGQGMNVSIQDGFNLAWKLASVLERRAPESLLATYAS
                     ERKVVAQNLIDFDKEWSGLLALPVDSWEDPAGLEQWYTSTLEFPAGFMTQYAPSMITL
                     GTEHQSLAPGFPVGKRFKSAEVIRRADNRWHHLGHFHEADGRWRVYVFADAAAPARTA
                     TPTADFAQWWSRDAASPRVRYTPADGDDDAIFDTKVIYQQDYTLVEPGDVPDAFMPTK
                     VPYGLIDINQVFASGHGR"
     assembly_gap    19490..19589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19705..20508
                     /gene="heR"
                     /locus_tag="PLW15_00910"
     CDS             19705..20508
                     /gene="heR"
                     /locus_tag="PLW15_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HOT32990.1"
                     /translation="MARTPRTAEENIRRLRPYNLVAGSLHLVQAVVFAVILARLTTQV
                     TFGVTADYLAGAPGFPIPPERVDLFAVNVGAGVVAFLALSALFHFLISSPAFFGRYSA
                     GLRQQHNYFRWVEYALSSSIMIWLIAQTTGITDVAALFAIFAANAAMIGFGALQEKYE
                     RPGSGNFLPFIMGSMVGVVPWIVILIYFFSPGSTSTVEPPAFVYGIIISLFVFFNTFA
                     INQVLQYRQVGPWRDYLFGERVYITLSLVAKTALAWQVFAGAIIPAFTS"
     gene            20581..20925
                     /locus_tag="PLW15_00915"
     CDS             20581..20925
                     /locus_tag="PLW15_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOT32991.1"
                     /translation="MTRIFGTPFAAVYPLYVAKLEKKDRTRAELDQAIEWLTGFDEPT
                     LKRLIDEDATFEQFFAAAHLNPNTTLITGVVCGVRVEEIEDPLMQKIRYLDKLVDELA
                     KGKAMEKVLRAP"
BASE COUNT         3475 a         7221 c         6884 g         3459 t
ORIGIN      
        1 gaactggagt tcgtactcag cggcggaggg ccgttcgggg catggctcta cgagaagttc
       61 acgaatcctc cgacggccat tcggatcggg gaccactggc cacagggcga cagtccggtc
      121 gtgctcacga cggcgctggg caccgagtcg agctcaaccc ggcgcctccc cgtaccgtgg
      181 atcgcggacg acctcagcta tggcgcacat ccgcacccgc gcgagatgat tctgtacgac
      241 cgcgtcgaca cccgcctgct gttctccgac ttcgccgcac gtatctccct gctggggagg
      301 ggctgagccc ccgaccgccg acagctaacc tgaaacgtgt gaccgcaccc gccgaatccg
      361 agaccgcatt ccgaccgtcg tggtggatac tcgcgctcgt cggggtgatc ggcggggtgc
      421 tctccggcgc gttcggcgtg gggggtggca tcctcatggt gcctctgctc atcgggttgg
      481 cgaagatgaa ccagcgccag gccgccgcga cctcgctgct cgcgatcgtg ccgacggcga
      541 tcgcgggatc ggccacctat ctgatcgcca gccaggtgga cgtgttcgcg gccctgttcc
      601 tcgggatcgg cggtgtggtc ggatcgctct acggcacccg tctactcaaa caactccccc
      661 tcggcgtgct gcggtggggg ttcatcgcgc tgctcgtggt cgtggccatc cgcatggtgc
      721 tcgtgattcc agaccgcagc tcggggcacc ttgagccgag cgtgctcgcg gcgctcggtc
      781 ttgtggcgat gggcctgttc gtcggcgtcg catccggtct gttcggaatc ggcggcggcg
      841 tgatcgctgt tcctgcgctg atcgcggtgt tcggcctggg cgacctgatc gccaagggaa
      901 catcgctgct gttcatgatt cccaccgccg tcaccggctc ggtcaacaac acgcgcaacc
      961 gcctggtcga cgtgcgggcg ggactcatcg tgggagtcgc cgcgacggtc gcctcattcg
     1021 gtggggcggc ccttgcgttc gtcatgtcac ccgcagtgtc gtcctacctg ttcgcggcct
     1081 tgctgctggt gtcggcagtg caattgaccg tgcgagcgat tcggatgcga cgagcctgag
     1141 cccgtcgcta cgcgtcccag attgcctcct cctcggctgc ggaggcgtac cagtcccgcg
     1201 acaggcgggc ggccatgtgc tccgcgtacc acgacgccca ctcgggatcc ggccgtccgg
     1261 tgacctgttc gtgcacaccg tgcgcggtgg cagcactgag aagcgccgag agaatgtcct
     1321 gctcccggta cgagtcgccg agcgcacgga ccattcgagc ggcgtaccac tggggccact
     1381 cgggtgcggg accatgggcc tcctcgtacg cacgatgcgc gacatccgtc gattcgagca
     1441 ggtcggtgag gacggcgacg aagccggcgt ccacactcat cgtcccggcg cccggttcac
     1501 gatctcctgc acgacccagc cgttgccgtc aggatcgctg aaggatacga acgatccgta
     1561 gtccgcccgg tccggcgcgg gtccctccgc gcgggcgcgg cctccgctgt ggtggaagat
     1621 gccacccgcg tcatgccaga cctcgctcac atcgattccc cggccggcga gttccgtgcg
     1681 agcgtgcacg atgtcgctca caaccaagtg gaggttctcc acagagcccg gcccggcatc
     1741 cgttattccg ctaccgatga tgatggaggc ctgcgatccg ggcggagtga actgaaccac
     1801 gcgaaagccg tcttctccaa caaagtcgat atcgagtcgg aacccgagtc tttcgtagaa
     1861 ctccttggcc cgatccacat cggtgacggg cacggtcagc acttcaagtt tcatttccat
     1921 ggtcgtccca accgcgcggg gcgaccctac gatctcgctg gcggctatgc cgtcagcgaa
     1981 cgtgtgcggg cgtgctagcg gcggttctct gcgcgacgga agtagacgcc ggcgaccacg
     2041 agcgcgattc ccagcgccac ggtgaggatc gtcgcgatgg ctccgagacc cagcgcggcg
     2101 gcaactccga gaaggatgac ggccgcgagg attgccgcaa cgacgaagat gatcgccgcc
     2161 gtgttggtga tctccttgcc cacgtacagc acgatcgcgc cgacgaggaa gcccagggcc
     2221 gcgatcacag cgccgattgt ggtcagaacg cccggaagac ccagacccag accggcgagc
     2281 gcgagaatcg cgaacccgac ggcgccgacg agcagcgaca cctttgcgat cgtgttgttg
     2341 acggcaccca gggcgaggat cacgaggccg acggcgagcg cggcgtaggc caaggctgcc
     2401 aaccagggca cggcgacccc ggccagaggg agcacgacgg cgagaaggaa cagcgcaccg
     2461 gaaactatga gagcgattcc tgaagctgat cggcgcgatc gggatagtgc ggccacggtg
     2521 tgctcctgac gtcgttgtgc gggtgtggcg tcagtctagg cccctcgctc aggccggatg
     2581 ggccccaacc accctcgagc gatgttgaca gcctcggcag atcgtatatt atttcaaata
     2641 ttcgatcgcg acgaggcgga gagttcatga gtacgtcaga ccttgggcac gacttccgcc
     2701 ccggcaagat catcgcgatg cacctcaact acccctcgcg gattgcccag cgcgggcggg
     2761 tccccgctca cccctcctac ttcctgaagc ccgctacgtc ggttgccgcg actggcggca
     2821 ccatcgaacg ccccgcgggc accgaactgc tcgccttcga aggcgagatc gctctcatca
     2881 tcggtcgtcc gacgcgtcgc gtgtcacccg aggagggctg ggatgcggtg tccggcatca
     2941 ccgcggcaaa cgacttcggg gtctacgacc ttcgctatgt ggacaaggga tccaacttcc
     3001 gctcgaaggg cggtgacggg ttcactccgc tcggcccgtc gatcatcgac gcccggtccg
     3061 tcgatcccgc gaaacttcga gtgcgcacct gggtgaacgg gaccgtggct caggatgaca
     3121 ccaccgccga cctcacgttc tccctcggcc tgctcgtggc cgatgtctcc cagctcatca
     3181 ccctcgagcc cggggacgtc atcctcacgg gaacgccggc gggctcctcc gtcgtcgtcc
     3241 ccggcgacgt ggtcgaagtc gaggtgacca ccgctgacgg tgccctgagc tcgggccgac
     3301 tcgtgaccac ggtgacgcag ggagtccacg agttgcctgc cttcagcgcc ggaccgaagg
     3361 tagacgacct ccagcgggag gaggcgtggg gatcacgcgc cgcggcgggc ctgcccgagg
     3421 aggcgagcat cctcacggag cacctgaagg ccaagatcaa ctcggtcgcg accgcgaccc
     3481 tgagctcact gctgcgcaag cgcggtttca acaacgtctc gatcgatggc ctgatcgcga
     3541 cccggcccgg tgcacgactg gtcgggcttg cgcgcaccct gcgctacatc ccgaaccggg
     3601 aggacctgtt cgatcgcttc ggcggcggat acaacgccca gaagcggctc ttcgactccc
     3661 tgaggcccga cgatgtcgtc gtcatcgagg ctcgtggcga ctccggttcg ggaaccctcg
     3721 gcgacatcct cgcgatcagg gcgaagcacc tcggagccgc cggaattgtc actgacggtg
     3781 gcgtgcgcga ccttgccgtg gtcaccgaac tcggcatccc gacctaccac gcgggaggtc
     3841 atccggcagt gctcggccgc aagcacgtgc cgtgggagta cgacaccacg atcggctgcg
     3901 gcggcgccgc cgtgcagccg ggggacgtca tcgtcggcga cgatgacggt gtactcgtga
     3961 ttccgccctc cctgatcgcc gatctcgtcg acgaggcgat cgcacaggag aaggaggagg
     4021 cgttcatcgc agcacacgtg gccgcgggcg aaccggtgga gaagctgttc cccctcagcc
     4081 ccgagtggcg cgagaagtac cgagccagtg tgggggagtc gtgaccgcat ccgtcggcat
     4141 caacaagtcg gagcgcgcct acctcgagat caagtcgaag atcaccgacg gcagcttcgg
     4201 tcccggctac cgcctggtgc tcggccagat cgccaacgag ctccaggtga gcgtggttcc
     4261 ggtgcgggag gcgatccgac gcctcgaggc agaggggctc gtcaccttca ccgcgaacgt
     4321 cggcgcccag gtagttgcca cggatgcccg cgaataccag tacacgatgg agaccctgag
     4381 cctggtcgag ggaatggcga cggcgatggc cgcaccgaac gtgacgcccg gcgatctcgt
     4441 gctggcgcgc tcgatcaacg tcgagatgcg ggagtgcctg gagcacttcg atcccaagcg
     4501 cttcacggaa ctcaaccacg agttccacgc ggtgatcttc gagcactgca gtaacctgca
     4561 catcctcgat ctggtgcacc gcgggtggaa caggctcgcg accatccgtg agtcgacgtt
     4621 cgctttcgtt cccgggcgcg cccagcgttc cgtcgaagag cacgaccaca tcctcgacct
     4681 catcgaggcg aaggcgccgg cgctcgagat cgagatcgcc gcccgctccc atcgcctcgc
     4741 gactcttcac gagtacctcg agtaccagaa gtcgcagcag tcctgagtcg cgacgcgacc
     4801 gaaacccata ccccttggag caccgatgac acagcaccac gttcccgaag accttcccac
     4861 ccatctccag cacttcatcg acggccagtg ggtcgacagc atcggcgggg agaccttcga
     4921 cgtgctcgac cccgtgtcca accagaacta cgcgacctgc gccgctggcc agaaggcgga
     4981 catcgaccgt gccgtcgccg cggcgaagca ggcattcgac agcgggccgt ggccgcgcct
     5041 gaagccgcgc gaccggttcc gcattctgaa ccgcatcgcc gacctggtcg aggagcgcga
     5101 tgcacgcctc gcagagctcg agaccttcga caccgggctg cccatcaccc aggcgctcgg
     5161 gcaggcccag cgcgccgcgg agaacttccg cttcttcgcc gacctgatcg tcgctcaggc
     5221 cgacgacgcg tacaaggtgc ccggcactca gctcaactac gtcaaccgca agccggtcgg
     5281 tgttgcgggc ctcatcacac cgtggaacac cccgttcatg ctggagagct ggaagctcgc
     5341 gcccgccctg gcctcgggat gcaccgtggt gctgaagccc gccgagttca ccccgctctc
     5401 cgcgagcctc tgggccgaga tcttccgcga ggccggtgtg cccgatggcg tgttcaacct
     5461 cgtcaacggc atcggcgaag aggcaggcgc ctctctcgtg gcccaccccg acgttcccct
     5521 catctccttc accggtgaga ccacaacggg caagatcatc taccgctcgt gcgccgacaa
     5581 cctcaagggc atgtccatgg aactgggggg taaatcccct gcggtcgtct tcgccgacgc
     5641 cgatctggat gccgcgatcg actcgaccct cttcggtgtc ttctccctca acggcgaacg
     5701 ctgcacggcc ggctcgcgca tcctcgtcga gcgctcgatc tacgacgagt tctgcacccg
     5761 ctatgccgag cgcgccaaga acatcgtggt cggcgacccg cacgacccga agaccgaggt
     5821 gggtgcgctc gtgcatcccg agcactacgc caaggtcatg agctacgtgg aggtcggcaa
     5881 gaccgagggt cgacttcttg cgggcggttc gcgcccggag gggctggacg agggcaacta
     5941 cgtctcaccg acggtgttcg ccgacgtcgc accgaccgcc cggatcttcc aggaggagat
     6001 cttcggaccg gtcgcggcga tcaccccgtt cgataccgac gaggaggccc tcgcgctcgc
     6061 caacggtgtg cgctacggcc tcgccggcta catctggacg aacgacctga cgcgcgccca
     6121 cacgttcgcc cagaatgtcg aggccggcat ggtgtggctc aactcccaca acgtgcgcga
     6181 cctgcgcacc ccgttcggcg gagtcaaggc cagcggtctc ggccacgagg gcggctaccg
     6241 cagcatcgac ttctacaccg accagcaggc ggtccacatc acgctcggac cggtgcacac
     6301 agcgcgtttc ggcgccaccc gatagcccca gacactcgaa gaggagaaca ccttgtccag
     6361 ccccatcccc gtccccacca gcccggcgcc cgacatcctg cgctgcgcgt acatggaact
     6421 cgtcgtcacc gacctggcca ggtcgcgcga gttctacgtc gacgtgctcg gactcgttgt
     6481 caccgaggag gatgacgagg ccatctacct gcgctccttc gaggagttca tccaccacaa
     6541 tctggtgctg cgcaagggac cggttgccgc cgtcgccgcc ttctcgttcc gagtgcgcag
     6601 tcccgaggac gtcgacgcag ccgaagcgtt ctacaccgag ctcggatgcc cgaccgaccg
     6661 tcgcgccggc ggcttcgtca agggcatcgg cgactcgctg cgcgtcgtgg atcctctcgg
     6721 ctttccctac gagttcttct acgagaccac ccacgtggag cgcctggcct ggcgctacga
     6781 cctgcagtcg cccggcgccc tcgtgcgcct cgaccacttc aaccaggtca cccccgaggt
     6841 gccgcgcgcc gtcggcttca tggagtcgct gggcttccgc gtcaccgagg acatccgcga
     6901 cgatcaggga acggtctacg cggcgtggat tcggcgcaag ccgaccgttc acgacaccgc
     6961 gatgacaggt ggcgatggcc cgcgcatgca ccacgtggcg ttcgcgaccc acgagaagca
     7021 caacatcatc gccatctgcg acaagctcgg agcgctgagg ctgtccgacc acatcgagcg
     7081 cggacccgga cgtcacgggg tgtccaacgc cttctacctg tacctgcgcg atcccgacgg
     7141 tcaccgcgtc gagatctaca cgcaggacta ctacaccgga gaccccgaca acccgctcgt
     7201 gtcatgggac gtgcacgaca accagcgtcg cgactggtgg ggcaacgccg tcgttccgag
     7261 ctggtacacg gatgcctcgc tcgtgctcga cctcgagggc aacgtgcagc ccgtggtcaa
     7321 gcgcgctgag ccgagcgaac ttgaggagac gatcggtgcc gacggcttct cctacactcg
     7381 caagggcgag acgaccgagg gcttcaagct cggtcatcag ctctgagaat ggtgtgacta
     7441 catgagcgga gcgcacgatg cttgacgacg ctaccttgac cgcgatcgcg gatgagctcc
     7501 tcgaagcaca gcgaagcggg gtgccgatcc ccctcatcac ggcccgccat ccgggcatga
     7561 ctgtcgagga ctcctacgcg atccagcgca tctgggccga ccgcaagcgt gccggcggca
     7621 gtcgccttgt gggccgcaag atcgggctca cctcgaaggt gatgcaggtg gccacgggca
     7681 tcagcgagcc cgactacggg gtgatcttcg acgacatggt ctaccaaacg ggtgcgcgca
     7741 tcgagatcga ccagtacatc aacacccgca tcgaggtcga actcgccttc gtcttgggca
     7801 gaccgctcag cggcccgaac tgcacaatca ccgatgtgct ggatgcgacg gcctacgtca
     7861 tccccgcgct cgagatcctc agctcgaaca tcgagatgga gggcaggacc atcgtggaca
     7921 cgatctccga caatgcggcc atgggggcga tggtcgtcgg cggcaccccg accaagccgt
     7981 ccgacgtcga cctgcggtgg gtcgctgccc tcctctatcg caacgagacg atcgaggaga
     8041 cgggggtggc ggcggcggtg ctgggtcatc ccgcggcggg ggtgcactgg ctcgccaaca
     8101 agatctacgc ccacggcgac tcgatggttg cgggcgagat catcctcgcc ggatcgttca
     8161 cccggccgat gtgggtgtac aaaggcgata ccgtccacgc cgactacgga ccactcggag
     8221 ccatcacatg tcagttcgtc tagagccacc gctttcgttc gcgtctcgcc ttcgggattc
     8281 gactcgcacc ctggtgggcg tctgggtgtg ctcgggaagc ccgacggtcg ccgagatctg
     8341 cgcggggtca ggagtcgact gggtcctcat cgattccgaa cacgccccca acggcctcga
     8401 gtcgatcctc gctcagctcc acgcggtgca gggctatccc gcgacgcccg tggtgcgcac
     8461 accgcacggc gatcccaacc tgatcaagca atacctcgac ctcggcgtgc agaacctcct
     8521 ggtgccgatg gtgaacactg cggccgacgc ggaacagctg gtgcgagcgg tgcgctatcc
     8581 cccgctcggg atgcgcggcg tgggcagcgc gctcgccagg gcctcccggt ggaaccgggt
     8641 cgacaactac ctgcaccggg cgggcgagtt catctccctg ttcgtgcagg tcgaaaccgg
     8701 cgccgccgtc ggcaacatcg acgagatcct gtcggtcgac ggcatcgacg gtgtgttcgt
     8761 gggcccgtcc gatctagccg cgagcatggg cctgctcggc caacaggacc accccgatgt
     8821 tgtcgccgcc gtcgagcacg tcatcgctcg tgcacggcag gccggtcgtc ccgtcggagt
     8881 gaacgccttc gcccccgctc tagccgagcg ctacatcgat ggcggtatcg acttcatcct
     8941 cgtggccgcc gatgtgtcgc ttctcgcgcg ctcgacggag gagatcgccg cgcgcttcat
     9001 cgccgacacg tctgacgagc gccgcaccag ctactgacgc gcgctgtcga ttcggcctac
     9061 cgtcgttcgt cgtcctcacg agcggacgcc accggggtga ccgcgtcggg aggtgtgcat
     9121 ggccgaggcg acggacgttc gcgacgagga gcaagcgggc cgggacatcc ggtggatcga
     9181 ccggatcccc gcgccggtgt ggatccgggt gctgtgatgc cggatgtcga aaaacggtgt
     9241 gccccttcgt cgccctgtca taacgtgtga tcaacgaagg agacaccgat gaagtacatg
     9301 atgttcgtcg ccaccgatcc ggaggccgag ccgtacgatc ccgcgcagga caacatccag
     9361 gagtgggtgg ccgagatggt cgcccgaggc gtcaacctgc acggtgatcg gcttcgtccc
     9421 atcgaggatg ccacgctcgt gcgggtgcgc aacggcgagg tcctggtcac cgacggaccc
     9481 ttcacggagt cgaaggaatg gatcgccggc tacgacatgc tcgaggccgc cgacctcgac
     9541 gaggcgatcg agatcgcctc gaagcacccg atggcgcgat tcgggcggat cgaactgcga
     9601 ccggtgtggc cgttcgagga gtagctccgg cggtcgctga cgcggtggcc accgcgcacg
     9661 gatcggagtg ggctcgcgtg gtgtccactc tgatccgtgt caccggcgac tggtccctcg
     9721 cggaggactg cacggcggag gcgttcgagg ccgcgctggc gacgtggcct cgcgacggca
     9781 tccccaccaa ccccggcgcg tggcttacca ctgtggcaaa gaaccgagcc ctcgaccgcc
     9841 tgcggcgcgc ggcgacattg gcccgcaagc tggaggagat gacggcggtg accgagttgg
     9901 agcacctcga ccccgagctg tccgacttcc ccgacgaccg actgcggctg atcttcacct
     9961 gctgccaccc ggcgctcagc ctcgaggcgc gtgtcgcgct gaccctgcgc accgtgggtg
    10021 gcctgacgac cgcccagatc gcgcgggcgt tcctcgtgtc ggaggcgacg atcgcccagc
    10081 gaatcgtgcg ggccaagcgc aagatcaccg aagcggcgat cccgtaccgg gtgcccgagg
    10141 cgcacctgct cgcagaacga ctcagcggag tgctcgcggt gctgtacctg gccttcaacg
    10201 agggctactc ggtggtggct cacacgagca tcgccgacga ggcgatccgc gtgacgcgcg
    10261 cgctcgcgca gctgatgccc ggcgagcccg aggtcaccgg gctcctcgcc ctcatgctcc
    10321 tgcaacactc gcgccgccgc gcgcgcctcg acgcagaggc agtgcccctc accctcgagg
    10381 agcaggaccg cagcctctgg gatgccgcgg ccatcgccga ggccggcgag ttgctcaacg
    10441 gcgcgggccg cggggccgaa cccggcccgt accggctgca agccgcgatc gcagccgtgc
    10501 acgcgaccgc ggactccgcg gccgcgaccg actggcagac gatcgtcgcg ctctacgacc
    10561 ggctgctgac cgtgacaccg agcccgatcg tcgagctgaa tcgcgccgtc gcggtgggca
    10621 tggcgagcgg cccggaggtc gggcttgcgg cgttggagcg ggtcgcggca tccggggccc
    10681 tcgatggcta ctatctgctg cccgcggcgc gcgccgacct gtttcgtcga ctcggcgact
    10741 acagcaaggc agcggccttc taccgtgttg cgttggctgg agcgcccacg caacccgagc
    10801 gggacttctt cgcccggcgc ctcgacgagg tgggcggcta ggcgcccacg taggtcgcca
    10861 ggtgctggcc ggtgagagtc gagcgagccg ccaccaggtc ggcgggcgtg ccttcgaaca
    10921 cgatccgacc gccatcgtgg ccggcgccgg ggccgaggtc gatgatccag tcaccgtggg
    10981 ccatgacggc ctggtggtgc tcgatgacga tcaccgactt gccggagtcg acgagccgat
    11041 cgagcaggcc gagcaggttc tccacgtcgg cgaggtgcag gcccgcggtc ggctcatcca
    11101 gcacgtagac atcgcccttg tcggccatct gggtggcgag cttgaggcgc tgccgctcgc
    11161 cgccggacag cgtggtgagc ggctggccga gactcaggta gccgaggccg acatccgcga
    11221 gcctggcgag aatcgcgtgc gcggcaggca gcttggcgtc gcctgcgccg aagaactcct
    11281 cggcctcggt aaccggcatg gccagaacct cgctgatgtc gcgcccaccg agcttgtacc
    11341 cgagcacgga cgcgtcgaac cgccggccct cgcagacctc gcacacgctg gagacgctcg
    11401 ccatgacgcc gaggtcggtg aagatcatgc ccgcaccgtt gcagttgccg catgcgcctt
    11461 cggagttcgc gctgaacagg gcgggcttca cgccgttggc cttcgcgaac gccgtgcgga
    11521 tcgggtcgag cagaccggtg taggtcgcgg ggttgctgcg acgcgacccg cggatggcgc
    11581 tctgatcgac ggcgaccacg ccctcccgct tcgacaccga gccgtggatg agcgagctct
    11641 tgcccgagcc cgcaacaccg gtgacgacca ccagcacccc gagggggatg tccacgtcga
    11701 cgccctgcag gttgtgtgag gtcgcgccgc gaacaggcat cgtgcccgtg gatgcccgca
    11761 ccgaagtctt gagcgaggcc cggtcatcca ggtgccggcc cgtcagcgtg ccgctggctc
    11821 gcagccccgc cactgttccc tcgtacacga gctcgccgcc cgcggtgccg gcgccgggac
    11881 cgaggtcgac cacgtggtcg gcgatctcga tggtctcggg cttgtgctcc accacgagca
    11941 cggtgttgcc cttgtcgcga agggcgagca ggagctcgtt catgcgctgg atgtcgtgcg
    12001 ggtgcaaccc gacggtcggc tcgtcgaaca cgtaggtgac gtcggtgagc gacgacccca
    12061 ggtgacggat catcttggtg cgctgcgact caccacccga gagggtgccc gacggccggt
    12121 cgagcgacag gtagccgagt ccgatcatca cgaaggaatc gaggatctgc tgcagggtcg
    12181 cgagcagcgg tgcgacggtc ggctcatcga gactgcgcac ccagtcggcg aggtcgctga
    12241 tctgcagcgc gcaggcatcc gcgatgttca gaccgccgat cttggatgag cgcgcgccgg
    12301 cgttgaggcg cgtgccgccg cactcggggc aggcggtgaa ggtcaccgct cgctcgacga
    12361 aggcgcggat atgcggctgc atcgcgtcga cgtccttgga cagcatcgat ttggtgacct
    12421 tggggatcag tccctcgtag gtcatgttga tgttgtcgat gcgcaccttg acgggctcct
    12481 tgtagaggaa gtccgctcgc tcctgctcgg tgtactcgcg aatcggcttg gccggatcca
    12541 tgaagccgga ggcggtgaag atgcgcacgt accatccgtc gacggtgtag ccggggatcg
    12601 tgagcgcgcc ctgttcgagc gacttggtgt cgtcgaagag ctgcgtcaga tcgatatcgg
    12661 tgacgttgcc cgtgccctcg cagcgcgggc acatgccgcc caggtaggtc tcgttctcga
    12721 tcacggtcgt ctcaccggtg ctgctggtgc gtacgccgct ggtctggctg gtgggaatgt
    12781 tgaaggagaa cgcgttcggc gaaccgatgt gcggctgacc caggcgggag aacaggatgc
    12841 gcagcatcgc gttcgcgtcg gtcgcggtgc cgacggtcga gcggatgttg gcacccatcc
    12901 gctcctgatc gacgatgatc gcggtggtca gcccctcgag cacgtcgaca tcgggccggg
    12961 actggctggg catgaagccc tgcacgaatg cgctgtaggt ctcgttgatc atgcgctgcg
    13021 actcggccgc gatcgtgccg aacaccagcg agctcttgcc cgaaccggac accccggtga
    13081 acagggtgag ccgacgtttc gggatgtcga cggtgacgtt gcgcaggttg ttctcccgcg
    13141 cgccctgcac gcggatcagg tcgtgggaat cggctgggtg gggggtcgcg gacatcgggg
    13201 ctcctcgctc gggctcccgg ccagactagc cgcagccccc gtcacggcac ttctcgaaaa
    13261 gtgatcgcta ccaggcgatg agtttctccg gccgcagctg gatgacgctc gagtacttcg
    13321 ccgccatggt ctcgagggtc aggctgatgc cggcaaggcc gccacggtac ttggcctcgt
    13381 acaccgcccc gatctcgtcg atccactcgg tcgccgggcg atccgagatc gtggcccggc
    13441 cctgcagcac cgtgaggtgt tcgccgtcgg tgccggcctc gagatgcacg agggcgtgat
    13501 cgttggcgcg caggttgcgc accttcgcgg tcgcgggctc gctgagcacg agcacactgt
    13561 cgtcgtgcac gatgaaccac accggcacgg catgcggaaa gccgtcgcga ccgatcgtcg
    13621 tgaaccacgc gatgcgttcc tcgccgaggc gagcaagggc acgcgcgtgc acctcgttgg
    13681 tggggtcgag aagagagagg gatgctgcgg gcatgagtcc agtctcgccc atccccacgg
    13741 cgtccggcag gggtcgctca gcgatgtatc gttaactatc gctgacagtc gccggcgacc
    13801 gcggagcatc cgccacctca gtccagaaag gacacgtcat gcgcacatca tcttctgccg
    13861 gcttcgacct gtgggaggcc gtcgacggtc tccgtgaggc gttcgccccc cgggtctcgg
    13921 gacgcatggg gcgcggcgac atccgcaccg ccatcctcgt cgccctcacc gaggagccga
    13981 tgcacggcta ccagatcatc cagtcgatcg agaagagcac gcgcggtgcg tggaagccca
    14041 gccccggttc gatctacccg acgctgcagc tgctcgccga cgagggactc gtcacctccg
    14101 agcaggtggg cgagcgcaag gtctactctc tgaccgacgc gggcaaggag gccgcggccg
    14161 agttctccag cggccccgag ccgtgggagc cgcgccgctc gtggcgtacc gagcacaacg
    14221 tcgcggtacc gaaagcgggc gcgaagctcg cacaggccat cgcgcaggtc gctcagggca
    14281 ccccggagca gaccgagcgt gcggtcgagg tcatcgacga ggcgcgtcgc aagatctacg
    14341 cgatcctcgc cgaggactga ggggatgccg cccgctagcg gcccactcac cgcaccaccg
    14401 accggccgcc cgttccgggc gcgctaccgc cgcatcatgc ggttcgcctc gcgcgccttg
    14461 gcgcaggcct ggtggttcga actggtgctg ccccggatcg gactcggccg gctcgccgcg
    14521 cgcggtcgag tacgccgcct cgagaaactc gccaatcgct tctatgtgct cgccgccgac
    14581 ctcggcggat tgatgatcaa ggtgggacag ttcctgtcgt cgcgcctcga catcctgccg
    14641 cccgagatca ccaaggcgct cgagggcctc caggacgagg tcgcgcccga gtcgttcgag
    14701 cgcatccgag tgcagaccga gaacgaactc gggatgccgc tggagctggc ctacgcctcg
    14761 ttcgagccgg tgcccatcgc cgcggcatcc ctcggacagg cccatcgtgc acgcctgtcg
    14821 ccggccatcg cggccgacct cggcttcgag gaggtcgtcg tcaaggtgca gcgaccgggc
    14881 atcgaggcga tcgtcgccgt cgacctcgcg gcgcttcggc gcgttgcccg ctggctctcg
    14941 cgcgtgcgcc tcgtgtcgtc acgcgccgat gcgcccgcac tcgtcgagga gtttgccgcc
    15001 accagcctcg aggagatcga ctacctgcac gaggccggca acgccgagcg cttcgccgcc
    15061 gacttcgccg acgacccgag cgtcggcgca cccgtcgtcg tctgggatcg caccgcgctg
    15121 cgggtgctca ccctctccga cgtgaccgcc atcaagatca cggatgtcgc atcgctcacc
    15181 gcagcgggca tcgaccccgc cgaggtcgct caagccctcg cgcgcgccac cttccaacag
    15241 atcttcgtgg ccggcttctt ccacgccgac ccgcacccgg gcaacatctt cgtcaccccc
    15301 acgcccggcg agggcgacgg ctggcgactg acgttcatcg acttcggcat gatgggcgag
    15361 atctccgact cgctgcggtc gggcctgcgc gaattcatcc tcgccgccgt tgctcgtgac
    15421 gggcgctccc tcatctccgc catggagaac ctcggcgtgc tgctgaagtc tgcggacacc
    15481 gaggagctcg agcgggcgat gacgacactg ttcgaccgct tcggcggcat gggcgtcgcg
    15541 gagctgcagc gggtcgaccc gcgcgaactc gagaggttcg cgctgcagtt cggtgagacc
    15601 atccgggcgc tgcctttcca actgcccgag agcttcctgc tgctcatccg caccatctcg
    15661 ctcatctccg gtgtgacgag cgccctcaac cgcgatttca acatgtggga tgccgtcgac
    15721 ccgttcgcac gcaccgtgct caacaacagc ggcacgggca ccatccgcga gctcgcccaa
    15781 caggccctcg gctacgcgaa cacgctcgcg cggttgccga agcgcctcga cgaactgacc
    15841 gcgcggatcg accgcgggca ggtcgccgtg cgcacccccg acctcgatcg gcgactgcgt
    15901 accatcgagc gatcggtggg tcgcgtcgcg tcggcgatcg tgttcgcggc actgctcttc
    15961 gcgggggtgc tgctgcgccc gagcgacccg gtgctcagct gggtgctgat gggggcatcc
    16021 gcgctgccgc tgctgcacgt gctggtcacc tggcgcgggc ggtgacccgg gcaagggcca
    16081 acgtgccggc tcgggccgcc gcggccgtcg gcctcgcctt cgcgctgatg ctcaccgggt
    16141 gcacctcgcc tcccagtgcc cccgacccgg cgcccgatgg cgcgccgttc cccgtcgatg
    16201 ccacggccga ctaccagctg ggcggcgcct atcctccccc ggccggcgtg acgctcgtgg
    16261 cccgcgacag caccgaggag cccctcgccg gtgcttactc gatctgctac atcaacggct
    16321 tccagactca accgggcgac caatggcccg acgacctcgt gctgcacgac gggcagcaga
    16381 gactcgtaga cccgaattgg ccggatgaga acgtgatcga catctcgacc gccgagaagc
    16441 gcgctgccgc ggcatcccga atcgagcagg cgatcgcgac ctgcgcggcc tcgggcttcg
    16501 atgcggtcga gttcgacaat ctcgactcgt acacccgttc agacgaccac ctgacgctcg
    16561 acgacgcgat cgcgttcgcg agcctgctgg ttgaccgggc gcacgaactc ggcctgtcgg
    16621 cgggacagaa gaacacctcg gagctcggcc aggtcgggcc ggacacgatc ggcttcgact
    16681 tcgcggtcgt cgaggagtgc gatcagttcg aggagtgcgg agcgtacaca gacgtctacg
    16741 gcgggcgagt gatcgacatc gagtacacgg acgcgctgcg gcgaccgttc gtcgaggtct
    16801 gcgcggatgc cgcgaccccg accatcacga tcctgcgcga tcgcggactc gcgccgcagg
    16861 gcgaccccgc gcaccactac gagagctgct gagaccggcg gtcacctcga acagtcgcga
    16921 gccgactaac ctcgggcgct actagcccgt cgcggagatg agagcggatg cggcttcgag
    16981 ccgatcgacg atgagctcga cctccacggg tcgcgtcgcg tagagcacgg cgatcgacgc
    17041 cgcacgaccc gaccgcaggg gcacagcgat cgcggacagg cctgggatga cctcgtcgtg
    17101 gctcagttcg tagcgtgccg gcgggtggtg tgccggatcc acgatggact ggatcacccg
    17161 tccgggtgat ccacgcccga tggggtgccg tcgacccggt cgttgcgcga cgaaggtggc
    17221 cgcattccga ggttcgacgc tcagcagtgt caccgcctcc tcgccatcga agaccacgag
    17281 gaacgccgtc atctggaggt catcggcaac tcgctgcagt tcgggcaatg ccgcggcctg
    17341 aaggtcacgg gacacgctct gaccgaaggc cgccaggcgc acgccgagag tgaggtcgcc
    17401 gctcaggctt cgttgcacga agcggtggcg ctccagcgtg cgcacgagcc tgtacgccgc
    17461 cgaccggttg actccgagcg cggcggcgag gtcgttgatg gtcagcggga tgcggctctc
    17521 gccgatgagc gcgagcgcgg cgaggccgcg cgatagcgtc tgcgatccgg cgtcggccgc
    17581 acgctcgtgc ttctccacgc acacatcctt ccctgacgct tccctcaggg gtcgacatga
    17641 cctacagtga aagccgttat ttgaccattg tattcatata gtgaacatgg atgcctgggt
    17701 cagtcaacaa ggttgttgat cagtgaggaa gaccagagat gcagatccac cttcgcggtt
    17761 acgaggccac cgatccgcgg atcaagcccg ccgcgggctt cggcatcgac cgaccggccg
    17821 agctccccga cgagatggat gttctcatcg tcggcaccgg gcccgcggca gttgtcgtcg
    17881 ccgcgcagct gtcgaggttc ccctccatca acgtgcgcgc catcgaacag cgcgcgggac
    17941 gcctcgaagt gggacaggcc gacggcatcc aggcccgatc ggtggagacc ttccaggcgt
    18001 tcggcttcgc caccgaagtc gtcgacgagg cctaccgcaa cgtcgagatg tgcttctgga
    18061 agcccgaccc cgacgatccc agcgtgatcg ttcgtgcctc acgcgccgcg gatgacgcga
    18121 cagggatgag cgagtttccg cacatctacg tcaaccagtc ccgaatcctc gactacttcc
    18181 tgcgggatgc ggcccgcgcg cccggccgcc tcgtgccgga ctacggaatc gagttcgtcg
    18241 attgcaccat cgacaccgag gccgagtacc cggtctccgc gcaagtccgc tacgtcgccg
    18301 gagatcgaga aggcgaaatc cgcaccgtcc gcgcgaagta cctcgtgggt ggtgacggag
    18361 cccgcagccg tgtgcgcacc gcgctcggtc accgcctgca cggagaggcc tccatgcacg
    18421 cctggggtgt gatggacatc ctcgcgaaca ccgacttccc cgacatccag gtcaaatgct
    18481 cgatccagtc gcacgaggcg gggaacatcc tcacgattcc gcgggaaggc ggatacctcg
    18541 ttcgcgtcta tgtcgacctc ggcgaggtgg gcgaggatga cgatggtgcc atacgggcga
    18601 ccccgatcga ggcgatcatc gcgaaagcga accgcatcat ccacccatac tcgctcgacg
    18661 tgcgggcggt gacctggtgg tcggtctacg aggtcgcaca ccgcatcacg gatggcttcg
    18721 atgatgttcc gcccgaactc gtcggcacac gccagccgcg cgcgttcatc atgggcgatg
    18781 cctgccacac ccactcggcc aaggcaggcc aggggatgaa cgtgtcgatc caggatggct
    18841 tcaatctcgc gtggaaactg gcctccgtgc tggagcgtcg ggcgccggag tctctgctgg
    18901 ccacctatgc cagcgagcgc aaagtcgtcg ctcagaacct catcgacttc gacaaggagt
    18961 ggtcgggact cctcgcgctg ccggtcgact cctgggagga tcctgcgggg ctcgagcagt
    19021 ggtacacctc gaccctggag ttcccggccg gcttcatgac acagtacgcg ccgtccatga
    19081 tcactctcgg cacggagcac cagagtctgg cgccgggatt ccccgtcggc aagcgattca
    19141 agtccgccga ggtgatccgc cgggccgaca accgatggca ccacctcggc cacttccacg
    19201 aggccgatgg ccggtggcgt gtctacgtgt tcgccgatgc cgcggctccc gcgcgcaccg
    19261 caactccgac cgccgacttc gcccagtggt ggtcgcgcga cgccgcgtct cctcgcgtgc
    19321 ggtacacgcc agcggatggt gacgacgacg ccatcttcga cacgaaagtg atctaccagc
    19381 aggactacac cctcgtcgag cccggggatg tcccggatgc tttcatgccg accaaggtgc
    19441 cctacgggct tatcgacatc aaccaggtct tcgcctcggg acatggccgn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgtgatctgt gtccgatcgt cggtgccgga
    19621 gcggtctggg ttgccgcggg acctatggca ctgggcatcc cgaacgaata gcggtgcact
    19681 gagatcgacc cagaggagct cgacatggca cgcacgcccc gcacggccga ggagaacatc
    19741 cgccgacttc gtccctacaa ccttgttgcc ggatcgctgc atctcgtgca ggcggtcgtg
    19801 ttcgccgtca tcctcgcgag gctgacgacg caggtgacgt tcggagtcac ggctgactac
    19861 ctggccgggg cgccggggtt cccgattccg cccgagcgcg tcgacctgtt cgcggtgaat
    19921 gtcggcgctg gcgtcgtcgc cttcctggcg ctctcggcgc tgttccactt cctcatctcc
    19981 tcgccggcgt tcttcggtcg gtactccgcc ggcctgcggc agcagcacaa ctacttccgg
    20041 tgggtcgagt acgcgctcag ctcgtccatc atgatctggc tgatcgccca gaccaccggc
    20101 atcacggatg tcgcggccct gttcgccatc ttcgccgcca acgccgcgat gatcggattc
    20161 ggcgcccttc aggagaagta cgagcggccg ggcagcggca atttcctgcc cttcatcatg
    20221 ggatcgatgg tcggcgtcgt gccgtggatc gtcatcctca tctacttctt ctcgcccggc
    20281 tcgacgagca cggtcgagcc gccagcgttc gtctacggca tcatcatctc gctgttcgtc
    20341 ttcttcaaca cgttcgcgat caaccaggtg ctgcagtatc ggcaggtcgg gccgtggcgc
    20401 gactacctgt tcggcgagcg cgtctacatc accctcagcc tggtcgcgaa gacggcgctg
    20461 gcgtggcagg tattcgctgg ggcgatcatc ccggcgttca ccagctgagg ccgtgaggcg
    20521 gatcacggcg agcccgcagt cgctggtcag ggtcgctcga cccgcctagc cttgggtgtc
    20581 atgacgagga tcttcggcac cccgttcgct gcggtctacc cgctgtacgt ggccaagctc
    20641 gagaagaagg accgcacgcg ggccgaactc gaccaggcca tcgagtggct caccggcttc
    20701 gacgagccga ccctgaagcg tctgatagat gaggacgcga cgttcgagca gttcttcgcc
    20761 gccgcacatc tgaaccccaa cacgaccctc atcaccggtg tcgtgtgcgg tgtgcgcgtc
    20821 gaggagatcg aggatccgct gatgcagaag atccgctacc tcgacaagct cgtcgatgaa
    20881 ctcgccaagg gcaaggcgat ggagaaggtg ctgcgcgcgc cctagcgacg cctgccgtca
    20941 gggcagcagg aaatccgtcg ccgcggcgaa gatacgagtg tgatcagctg ggttctggag
    21001 ggactcaacg ccccatatcc gcacctgcga tggtgcgggt gagggtccag atcccagtgt
    21061 tttgctgggt tttttgacag ctcgacgtca ctgtgatcag gtgggttccg actcaagtgc
    21121 agtcaaaatg cagtcaaaa
//
LOCUS       DAOSOU010000008        20270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2640_length_20270_cov_6.326094, whole genome shotgun sequence.
ACCESSION   DAOSOU010000008 DAOSOU010000000
VERSION     DAOSOU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 20270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20270
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_20270_cov_6.326094"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLW15_00920"
     CDS             complement(<1..798)
                     /locus_tag="PLW15_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT32992.1"
                     /translation="MSSRVLGLDVVVPVYNEEGSLERSVRVLHSTLRSQFTIDWRITI
                     ADNASTDRTAEIARRLSAEFPEVELLHLAEKGRGRALKKAWLESPAEVVAYVDVDLST
                     DLRALPPLIAPLLSGHSDVAIGSRLRSGSRVVRGAKREFVSRTYNLLLRRTMNVHFSD
                     AQCGFKAMRRDAAHAILPHVQDTGWFFDTELLIVAERSGLRIHEVPVDWIDDEDSKVD
                     VVATAMDDLRGLVRVTRSIVAGRIPLEQIYGPVEVLKGVNLRAYAGKV"
     gene            complement(868..2025)
                     /locus_tag="PLW15_00925"
     CDS             complement(868..2025)
                     /locus_tag="PLW15_00925"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOT32993.1"
                     /translation="MSDEQYAFPTRAIRAGQEFDPTTGAVVPPIYMTSTFVQDGIGGF
                     RGGYEYARGGNPTRDSLQTLLASLEGGSHAYSFASGLAAEDTLLRAVLHPGDHIIMGN
                     DVYGGTHRLVSRVFVPWGVSISTVDMSDLDAVRHAIRPDATHVLWVETPSNPLMKISD
                     IAALAEIGHAAGALVVVDNTFASPYLQRPLELGADVVVHSTTKYLGGHSDVLGGALIV
                     DNPELAEKIGFLQFGVGAVSSPMDAWLTVRGIKTLAVRVDRHSSNAQTIAEALVGHPK
                     LDAVYYPGLPDHPGHELAARQMKAFGGMISVAVAGGAAKAKAFAEGTELFQLAESLGG
                     VESLIGYPAEMTHASVKGTALEVPDNVVRLSVGIEDAADLTADVLGALDRL"
     gene            complement(2083..3447)
                     /locus_tag="PLW15_00930"
     CDS             complement(2083..3447)
                     /locus_tag="PLW15_00930"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HOT32994.1"
                     /translation="MKYANTVTDLVGNTPLVRLNRVTDGIAATVLVKVEYLNPGGSSK
                     DRIATRIIDDAEAKGLLKPGGTIVEPTSGNTGIGLALVAQQRGYRCVFVLPDKVGEDK
                     RNVLTAYGAEIVVTPTAVAPESPESYYSVSDRLARDIPGAFKPNQYSNPNGPLSHYET
                     TGPEVWRDTEGKVTHFVAGVGTGGTISGVGKYLKEVSNGAVRIIGADPEGSVYSGGTG
                     RPYLVEGVGEDFWPTAYDPSVVDEIIASSDAESFEFTRRLAREEGLLVGGSSGLAVAS
                     GLKAARDLGPDDVMVILLPDGGRGYLGKIFNDKWMRSYGFLSAGDDRTVADMVGTKNT
                     DLAGLVHVHPSDTVRHTIDKMRQYDISQMPVLTAEPPVVMGEVVGSIDERALIDAVFS
                     GRAQLTDALAEFVGPPLGLIGMQDSVETAREALTKSDALLVTSDGKPAAVVTRHDLLA
                     FLST"
     gene            complement(3693..>5133)
                     /locus_tag="PLW15_00935"
     CDS             complement(3693..>5133)
                     /locus_tag="PLW15_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOT32995.1"
                     /translation="PMTDIWVQPWFWPAVVVVIGLPVTLLVLTEIQSSLERRGSRAAR
                     IVLLLRNYVAPVGALLLLLSQARYASVDLNWTQITGTVFGFLLILVLLNGLNYALFVT
                     AKETSWRSKVPSIFVDIVRVILILVSLAVLFSVVWGADVGGLFTALGVGSIVIGLSLQ
                     NAVGSVVSGLLLLFEQPFKLGDWIETGGLRGRIVEVNWRAVHLKTSRGVQIIPNASLA
                     GSSFMNLTRNDAPFEASLTVKFATDDAPDVVIDVCTRVAADLPFAAPGEPATVTPIEK
                     AKYEVDIPIVSPGNNSATLRQFRTRLWYAARRADLHLDGDLTDNFNTSENVLAQLKAF
                     SPALYLAESDLAVIASQVRLERYGAGEAVERPLVVPDGMRYVISGTAALYASLESGIE
                     VVFANLSAGDVMGLTALTRQGVSARITATSDLSVLFVPTAVLDALVTSRPRLARDIGL
                     EIDNRLSRAKAALAAAGVEVDLDSSRLIA"
     assembly_gap    5134..5233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5318..7504)
                     /locus_tag="PLW15_00940"
     CDS             complement(5318..7504)
                     /locus_tag="PLW15_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT32996.1"
                     /translation="MSNAGVTPPPAEGSIVLPSTTMKAGLSIRSLLLIMLLLVSVASS
                     VVVGLIGYVNGRDSLRDSAVNSLIEVRDSRAREITSLFTSMENTLLLNARGQSVANAM
                     QAFTAGFQELESTTLTDEEQASLEDYFTTQFGAQLSASGAAGDGEPIDASTFIPTGAA
                     QSYLTLHYTVPFDNFTDAIAVDDAGDGSTWSAAHAQYHDFFRRMTQLFKYEDVLLVDA
                     NGNIVYSAFKGVDLGSNLDSGPLQRTNLAKAYTEAMNGNIADTVVLTDFGSYPPSLGV
                     PAAWAVVPVAVNGTIIGTLAIEMPIDEINAVMTGGGDWAGSGLGRTGETYIVGADDKL
                     MRSLSRDLIENPDQYKEQAVAAGTAPGVAQQVIDGGSTLLLQPVRTDAVASAASGVTG
                     TTLAAGYLGGETIAAYAPLDIDGLDWVIVAEIDSAEAFAPVDAFTRNLLLSSAVIVLV
                     VSLLSLVIAGVIVRPLRRLRDAARRIASGETGVQVDAGTSDELADVGAAFNDMSRSLQ
                     VKEALLKSQQDENERLMLSLMPEPLVRKYREGARTIAQDHQEVTVMFGDIVGFEAFSR
                     NMESEKALELLNEILRSFDEAADRFGIERVRTTRSGYLASCGLSIPRVDNARRTVDFA
                     IELQRILERFGGKHGTALNLRAGIDTGTVTAGLVGETHIAYDLWGDAVNLAFQIQGNN
                     NDAGIFLTQAVVDRMPDTVHVRDWGYVTTATGAQRVWRIDPDDTRA"
     gene            complement(7840..>8573)
                     /locus_tag="PLW15_00945"
     CDS             complement(7840..>8573)
                     /locus_tag="PLW15_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT32997.1"
                     /translation="QFDDVVFRYPDAPADSPATLKGVSFTIEPGQFAAFVGPSGAGKT
                     TVSYLIPRLHDVTSGRVLFAGDDVRALEQDSLVRNIGIVSQETYLFHATIAENLRYAK
                     PGATDEELTAAARAANIHDTIASFPDGYDTVVGERGYRLSGGEKQRIAIARVLLKDPA
                     VLILDEATSALDSLSERVVQTALDAASRGRTTIAVAHRLSTIVAADVIFVVVAGRVVE
                     RGTHPELLAAGGMYASLYEQQVQGA"
     assembly_gap    8574..8673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8674..9848)
                     /locus_tag="PLW15_00950"
     CDS             complement(<8674..9848)
                     /locus_tag="PLW15_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT32998.1"
                     /translation="MSGMRGGGRGGRISGGDVEAQRAANATAPKIPDLLKRISQLFAP
                     HRAAITVTIILVLVSAAISVFPPLLTKQAFDVGLFPPSGTPNIPVLVELVLLMVALWI
                     SSAALGVWQTYLTATVGNKVMGALRIRLFGHLQAMELAFFTRTKTGVIQSRLQNDVGG
                     VAGVLSNTISSVIGNTVTVIAAFVSMLVLSWQLTVVAVILLPLLVIAQRKVGQVRARI
                     ATKTQESLSEMTAITQEALSVSGILLAKSFNQQQAEVGRYTRENENQIRLQVSQQMSG
                     QWFFAMVNIFLSIIPAVIYLVAAWLINGGTDVTAGTIVAFTTVQARLMFPLLGLLRVA
                     LDLQTSSALFARIFEYLDLVPAIVDRPDARPVRPKKLGEVQFDDVVFRYPDAPADSPA
                     "
     gene            complement(9950..10921)
                     /locus_tag="PLW15_00955"
     CDS             complement(9950..10921)
                     /locus_tag="PLW15_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT32999.1"
                     /translation="MTAIEEIAPVNLTPARTKSKGIRLTSRKQNNIFGSPIASFFAIV
                     IALVWTIPTFGLFISSIRTPADINNTGWWTFFTNPSFTLENYNTVLFEGSSVTPPLSL
                     YFLNSFAITIPATVFPVVIATFAAYALAWFDFKGRDLLFYTIFAMQVIPLQLALIPLL
                     QLFANGLKIGDATLIPPLGITGTYIPIWIAHTMFAMPIAIFLLHNFLSQIPRELMEAA
                     RVDGADAFKTFRMVVLPLSIPAIASFAIFQFLWVWNDLLVGLTFGGGKREIAPLMVRL
                     AELTGTRGGDWELLTAGAFVSIIVPVAVFFALQRYFVRGLLAGSVKG"
     gene            complement(10921..11919)
                     /locus_tag="PLW15_00960"
     CDS             complement(10921..11919)
                     /locus_tag="PLW15_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT33000.1"
                     /translation="MWIVTARSPFATVLPNIVLLLVGLVVFVAIIVLIFVLSERTKEK
                     TQNRLKYFVFLGPALLLLIIGLIYPAIRTLYLSFFDARSENFIGLQNYIWAFTQPEIL
                     IVLRNTLIWTIVVPIASTTIGLAIAYLTDRMKYAGLVKSLIFMPMAISFVGASIIWKF
                     VYTYQPDPNKADIGLLSAIFKAIGLTPPNWLLEAPLNTFLLIAVMVWIQTGFAMVVLS
                     AAMKAVPDDIIEASMLDGAKGWARFTRITIPMIWGSVIVVLTTITVTSLKVFDIIRTM
                     TGGNFQTSVIANEMYQQSFRALNYGTGSALAMILFLGVLPLIIFNVRQVRKEREVH"
     gene            complement(12020..13369)
                     /locus_tag="PLW15_00965"
     CDS             complement(12020..13369)
                     /locus_tag="PLW15_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33001.1"
                     /translation="MRAIKRRSIVLATTSATAFALILTGCSAPSGSGDIPAGCEAYAD
                     YQGNSGTEVEIYTTVVSPEAELFQESFTEFEDCTGITINWNGSKEFEAQLPVRVEGGT
                     APDLAVFPQPGLLAAMAATGKLLPASDAVAASVEANYSPDWKNYGTVDGTFYASPLGA
                     NVKSFVWYSPKTFKDNGWDIPTTWDELLALSDKIATTSDVQPWCAGFESGDATGWVGT
                     DWMEDIVLRFGGAEVYDKWVSHEIPFNDPAIVEAIGKAGQILKNPEYVGDVPSIATTS
                     FQTGGAGIVDGSCAMHRQASFIGGILVADQGATIVTPDDTTTEGGITTFYFPGATADD
                     KPALGGGEFIGAFADRPEVQAVQLYLTTPEWNNKKAALGGWFSANLGLDTANVADPVN
                     ANAVEILKNATTFRFDGSDLMPAAVGAGSFWKEMTAWVAEDKSDKAVLDAIEASWPK"
     gene            complement(14100..14492)
                     /gene="rplL"
                     /locus_tag="PLW15_00970"
     CDS             complement(14100..14492)
                     /gene="rplL"
                     /locus_tag="PLW15_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT33002.1"
                     /translation="MAKLTSDQLIEAFKELTLIELSEFVKKFEETFEVTAAAPVAVAA
                     APAAGGAGGAAEEVEEKDTFDVVLESAGEKKIQVIKEVRTLTSLGLGEAKALVDGAPG
                     VVLEGANKETAEKAKALLEEAGATVTLK"
     gene            complement(14562..15071)
                     /gene="rplJ"
                     /locus_tag="PLW15_00975"
     CDS             complement(14562..15071)
                     /gene="rplJ"
                     /locus_tag="PLW15_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT33003.1"
                     /translation="MANKEASVAELTDKFRTSNAVLLTEYRGLTVAQLKSLRKSISEH
                     ATYAVVKNTLTKIAANAAGITSFDDDLVGPSAIAFVHGDTVAVAKTLRDFAKANPLLL
                     VKNGYLDGNPLTADEVKKLADLESREVLLAKFAGAAKASLFGAAYLFNAPLSQAVRTV
                     DALREKQSA"
     gene            15353..16111
                     /locus_tag="PLW15_00980"
     CDS             15353..16111
                     /locus_tag="PLW15_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33004.1"
                     /translation="MDSAFPVVFVHGLFGSFAEPAATRHLGPAEFSAPDLRGYGHETE
                     VDVSLEGQVAALRSHVESRHPETPVHLVAHSIGAVYAFALADESPDLVESVTTVEGNF
                     TLADAFWSRSIAALDEDRARSEIEGRLRDPRAFLAGDGIEATDEHLAKAVDALAYQPW
                     RTVWMSASAIVEATSKPEYQELLRRVFARHRVHLVAGERSAGGWDVPGWAREAAASST
                     VIPGVGHMMMLENPDAVGRVLGQLLFTPEGRRTP"
     gene            complement(16108..17094)
                     /locus_tag="PLW15_00985"
     CDS             complement(16108..17094)
                     /locus_tag="PLW15_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33005.1"
                     /translation="MPEHTRRRSHSRARIGAALTLLAAFALALVGAPAATASQGSVPA
                     EVSAYAADPNGLVSRLDDLFGIGSGGAGIDFNETTAVGQLNRVFTFTEAFVAGVATDT
                     PVERQNLWTAPITVNDDTIGLAIIWINPASVAPELADFVRDPDLARALSDVPADSYVV
                     RDEQRAAWFTLGADELIPLVAGTSGLSGPIPLDDFQRMMIDRSGEPVDAPENSSLITP
                     VAVIIVALVGVVVILLVPQLLARRANRRGGVAAQAEPTPTPEPEPEPTPEPPAPKKPR
                     APRSPAKSKPSIESAPEAATEAPAEPTADAESTPAPPKKPRAPRRPATPPEA"
     gene            complement(17138..18595)
                     /locus_tag="PLW15_00990"
     CDS             complement(17138..18595)
                     /locus_tag="PLW15_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT33006.1"
                     /translation="MTRRLTLRRRLVFGIVALLALVTVLIGVFSVFALQGFLVGRLDA
                     QLTAATGRSQEAFEGRPNDDFGGRPPAGEVLALPGQGAGTLAGLVASNQLTTAAVLDQ
                     SGVPISLDATQLASLGTVQPGAAPVTVDLGGTLGQYRVIMQKTSASGVSLLIGLPLSE
                     VQAIVLRLTLLIAVIGILGVAAAAIAGAVVVRLALRPLERVASTATRVAELPLDRGEV
                     ALAERVPAQDADPGTEVGQVGAALNTLLEHVASALTARQASENKVRTFVADASHELRT
                     PLASIRGYAELTRRGRHELPDDVVHAIGRVESESVRMTALVEDLLLLARLDEGAAIEG
                     ATVDLTRLVIDALSDARAAGPDHIWQLELPDEAVQVHGDQPRLHQVVANLLANARTHT
                     PAGTVVTAALIRGDGWVTIEVRDTGPGIDPALTPTIFERFVRGDGSRSRAAGSTGLGL
                     AIVTAVVEAHGGRAEVESAPGSTVFRIILPIGEIA"
     gene            complement(18592..19344)
                     /locus_tag="PLW15_00995"
     CDS             complement(18592..19344)
                     /locus_tag="PLW15_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33007.1"
                     /translation="MTTSNHAPAIAAQPRISRADGSPIRAVVVDDEDSLTDLLSMALR
                     YEGWDVKLASDGQKALSVIREFRPDVVVLDIMLPDIDGLTVLSRLRADGIQVPILFLT
                     AKDSVDDRVAGLTVGGDDYVTKPFSLEELVARLRGLIRRSALAVSDSADSVLEVGDLR
                     LDEDSYEVSRAGAAIELTATEFELLRFLMRNPRRVLSKAQILDRVWSYDFGGRSSVVE
                     IYISYLRKKIDTLGEPMIHTVRGFGYVLKPAG"
     gene            19480..>20270
                     /locus_tag="PLW15_01000"
     CDS             19480..>20270
                     /locus_tag="PLW15_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33008.1"
                     /translation="MFGTYLFRELTNRRKQTAIIAIGMALAIALVIIVNGVSAGVSAA
                     QAKVLESVYGVGTDITVSQQPTGPTEGGAAGGPPSFDFGSGDGSTADGSTQLSQARLT
                     TGPGSATFADTALATVQGVDNVSSATATLSLTNINFSGEIPDFQAGGGGLPTDGTAPP
                     TGGADGAGGSSFDIESITVTGIDVTGDAVGPLTAVSLTDGRSFDAGDVGTNVVVLDSS
                     YATTAELGVGDTLGLGGEDFEVIGIVASTSSDASSTNTYIPLDTA"
BASE COUNT         3434 a         6423 c         6876 g         3337 t
ORIGIN      
        1 gaccttgccg gcataagcgc ggaggttcac ccccttgagc acttccaccg gaccgtagat
       61 ctgctccaac gggatgcgcc cggccacgat cgagcgggtg acgcgcacga ggccgcgcag
      121 gtcgtccatc gcggtcgcaa cgacatcgac cttgctgtcc tcgtcgtcaa tccagtcgac
      181 cggcacctcg tggatgcgca acccggagcg ctcggcgacg atcaggagct cggtatcgaa
      241 gaaccacccg gtgtcctgca cgtgcgggag gatcgcgtgg gcggcatccc ggcgcatggc
      301 cttgaagccg cactgggcat cgctgaagtg cacgttcatc gtgcgtcgca gcagcaggtt
      361 gtaggtgcgg gagacgaact cccgcttcgc gccgcgcacg acccgcgatc cggagcgcag
      421 tcggcttccg atcgcgacat ccgaatggcc ggagagcaac ggtgcgatca gcgggggcaa
      481 cgcccgaagg tcggtggaga ggtcgacatc gacgtaggca accacctcgg ccggggactc
      541 cagccacgcc ttcttcagcg cacggccgcg gcccttctcg gcgaggtgca gcagctcgac
      601 ctcggggaac tcggcgctca gacgtcgcgc gatctcggcc gtgcgatcgg tgctggcgtt
      661 gtcggcaatg gtgatgcgcc agtcgatcgt gaactgcgac cgcagggtcg aatgcagcac
      721 ccgcacgctg cgctcgagcg acccctcctc gttgtagacg ggaacgacga catccaggcc
      781 cagcacgcga ctactcacct agtgactgtc actccatgcg ctggacgcca gccccggatt
      841 gcctatggct gggctgtgag tgtggggcta cagccggtcg agagcaccca ggacatcggc
      901 ggtcaggtcg gcggcgtcct cgatgcccac cgacagccgc acgacgttgt caggaacctc
      961 gagtgctgtg cccttcaccg aggcgtgagt catctcggcc gggtagccga tgagcgactc
     1021 gactccaccc agggactcgg cgagctggaa gagctcggtt ccctcggcga acgccttggc
     1081 cttcgccgcc ccgccggcga cggcgaccga gatcatgccg ccgaacgcct tcatctggcg
     1141 cgcggcgagt tcgtggcccg ggtgatccgg caggcccggg tagtagacgg catccagctt
     1201 cggatgcccg accagggcct ccgcgatcgt ctgcgcgttg gaggagtggc ggtcgacacg
     1261 caccgcgagc gtcttgattc ctcgcacggt gagccaggca tccatcggtg acgacacggc
     1321 accgacaccg aactggagga agccgatctt ctcggccagc tcggggttgt cgacgatgag
     1381 ggcgccaccg agcacgtcgg agtggccccc cagatacttc gtcgtggagt gcacgacgac
     1441 gtcggcgccc agttcgagcg ggcgctgcag gtagggcgag gcgaaggtgt tgtcgacgac
     1501 gacgagcgcc ccggccgcgt gaccgatctc ggcgagcgcg gcgatgtcgc tgatcttcat
     1561 gagcggattc gacggggtct cgacccacag cacgtgggtg gcatcgggcc tgatcgcgtg
     1621 gcgcacggcg tcgagatcgc tcatgtcgac cgtggagatc gacacgcccc acggcacgaa
     1681 cacgcggctc acgagccggt gggtgccgcc gtagacgtcg ttgcccatga tgatgtgatc
     1741 gccggggtgc agcaccgcgc gcagcagggt gtcctcagcg gcaaggcccg acgcgaaact
     1801 gtaggcgtgg ctgccgccct cgagcgaggc gagcagggtc tgcagcgaat cacgcgtggg
     1861 gtttccgccg cgggcgtact cgtagccgcc gcggaagccg ccgatgccgt cctgcacgaa
     1921 ggtcgacgtc atgtagatcg ggggaaccac cgcaccggtc gtggggtcga actcctggcc
     1981 ggcgcggatc gcgcgggtgg ggaaggcgta ctgctcgtcg ctcatgatgg ctctcgttgc
     2041 tgtggttgga tcaggcacag ggtggatcag gtgcagggtc gatcaggtgc tcaggaaggc
     2101 gagcaggtcg tgccgggtga ccacggcggc gggcttgccg tcgctggtca cgagcagggc
     2161 gtccgatttg gtgagcgcct cacgcgccgt ctcgaccgag tcctgcatac cgatcaaacc
     2221 gagcgggggg cccacgaatt ccgcgagggc atcggtgagc tgggcgcggc cgctgaacac
     2281 ggcgtcgatc agggcgcgct cgtcgatcga gccgaccacc tcgcccatca cgaccggggg
     2341 ttcggcggtg agcaccggca tctgggagat gtcgtactgg cgcatcttgt cgatggtgtg
     2401 acgcacggtg tcgctggggt gcacgtgcac gagtccggcc aggtcggtgt tcttcgtgcc
     2461 caccatgtcg gcgaccgtgc gatcgtcgcc ggccgacagg aatccgtagg agcgcatcca
     2521 cttgtcgttg aagatcttgc cgaggtagcc gcggccgccg tcgggcagca ggatcaccat
     2581 gacgtcgtcg gggccgaggt cccgcgccgc cttgagaccg gatgccacgg ccagcccgct
     2641 cgagccgccg acgagcagcc cctcctcgcg cgccagccgc cgggtgaact cgaaggactc
     2701 agcatcggac gacgcgatga tctcgtcgac cacgctcggg tcgtaggcgg tcggccagaa
     2761 gtcctcgccc acaccctcga cgaggtaggg gcgcccggtg ccgccggagt agaccgaacc
     2821 ctcggggtcg gccccgatga tgcgcaccgc gccgttcgag acctccttga ggtacttacc
     2881 gacgcccgag atcgtgccgc cggtgccgac gccggcaacg aagtgcgtga ccttgccctc
     2941 ggtgtcgcgc cacacctcgg gacccgtggt ctcgtagtgg ctgagtggcc cgttggggtt
     3001 ggagtactgg ttgggcttga acgcacccgg gatgtcacgg gcgaggcgat cgctcacgct
     3061 gtagtacgac tcgggactct ccggcgcgac cgccgtgggc gtcacgacga tctcggcacc
     3121 ataggccgtc agcacgttgc gcttgtcctc gccgaccttg tcgggcagca cgaacacgca
     3181 gcggtagccg cgctgctggg caaccagtgc cagcccgatg ccggtgtttc cactggtcgg
     3241 ctcgacgatc gtgccgccgg gcttcagcag acctttggcc tcggcgtcgt cgatgatgcg
     3301 ggtcgcgatt cgatccttcg acgagccgcc ggggttgagg tattccacct tgacgagcac
     3361 cgtcgcggcg atgccgtccg tcacgcggtt caggcgcacg agcggggtgt tgccgacgag
     3421 gtcggtgact gtgttggcgt acttcatgat gtcgccaatc taccagtggg gtgcgcggcg
     3481 ttacggggcc gcccgcgggt ggggtcgccg cggcatccgg accgcttgcc gctcgccgtc
     3541 cgccgtccgc cgctcgccgc tcgccgtccg cggctcgccg ttcaggacat cactgcaaca
     3601 cgccgagcga cgagatgcag acacaagcgt gtcgcgcaga tctcctgatc cgcgaagggg
     3661 ggtaggaggg gcgacgggat gccaggctgg cgttacgcga tcaggcgcga gctgtcgagg
     3721 tcgacctcga caccggccgc ggcgagcgca gccttggccc gactgaggcg attgtcgatc
     3781 tccaggccga tgtcccgagc aagtcggggt cgggaggtca cgagcgcatc gagcacggcg
     3841 gtcggcacga acagcaccga cagatcggac gtcgccgtga tccgcgccga gacgccctgg
     3901 cgcgtcagcg ccgtgagtcc catgacatcg ccagcggaca ggttggcgaa gaccacctcg
     3961 atcccggact ccagtgacgc gtacagcgcc gcagtgcctg agatgacgta gcgcatcccg
     4021 tcgggaacga cgagcggccg ctcgaccgcc tcccccgcac cgtaacgttc gagtcgcacc
     4081 tgggaggcta tgaccgcgag gtcgctctcg gcgagataca gcgcggggct gaatgccttc
     4141 agctgggcga gcacgttctc ggaggtgttg aagttgtcgg tcaggtcgcc gtcgaggtgc
     4201 agatcggcgc gtcgcgccgc gtaccagagt cgggtgcgga actgtctcag ggtcgccgag
     4261 ttgttgccgg ggctgacgat cggaatgtcg acctcgtact tcgccttctc gatcggcgtg
     4321 accgtcgccg gctcgccggg agccgcgaac ggcaggtcgg ctgccactcg ggtgcacacg
     4381 tcgatgacga catcgggcgc gtcatccgtc gcgaacttca cggtgaggga tgcctcgaac
     4441 ggtgcgtcat tgcgagtgag gttcatgaac gagctgcccg cgagcgaggc gttcgggatg
     4501 atctgcacgc cgcgcgaggt cttgaggtgc accgcccgcc agttcacctc gacgatgcga
     4561 cctcgcaggc cgccggtctc gatccagtcg cccagcttga agggctgctc gaacagcagc
     4621 agcagaccgg acacgaccga tccgacggcg ttctgcagcg agagaccgat gacgatcgag
     4681 ccgaccccga gggcggtgaa cagtccgccg acatcagctc cccacacgac cgagaagagc
     4741 acggcgagac tgacgagaat gaggatgacg cgcacgatgt cgacgaagat gctcggcacc
     4801 ttgctgcgcc agctcgtctc cttcgcggtg acgaacaggg cgtaattcag gccgttgaga
     4861 agcaccagga tgagcaggaa gccgaacacc gtgccggtga tctgcgtcca gttcaggtcg
     4921 accgacgcgt agcgcgcctg gctgagcagc agcagcagcg caccgaccgg cgcgacgtag
     4981 ttgcgcagga gaagcactat gcgcgcggcc cggctgccac gccgctccag actgctctgg
     5041 atctcggtga gcacgagcag cgtcaccggc aggccgatga ccaccacgac cgccggccag
     5101 aaccacggct gaacccagat gtcagtcatc ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnngaccacc acgaccgccg gccagaacca cggctgaacc cagatgtcag
     5281 tcatcggttc tcaccgacga tcggcgctca tgatcgctca cgcgcgggtg tcgtccgggt
     5341 cgatgcgcca cacccgctgc gcacctgttg cggtagtcac atagccccag tcgcgcacgt
     5401 gcaccgtgtc gggcatccgg tcgaccaccg cctgggtgag gaagatgccg gcgtcgttgt
     5461 tgttgccctg gatctggaag gcgagattca ccgcgtcacc ccacaggtcg taggcgatgt
     5521 gggtctcgcc gacgaggccc gccgtgacgg ttccggtgtc gatgccggcg cgcaggttga
     5581 gcgcggtgcc gtgcttgccg ccgaatcgct cgaggatgcg ctgcagctcg atcgcgaagt
     5641 ccacggttcg gcgggcattg tccacccgcg gaatggacaa cccgcagctg gcaaggtacc
     5701 ccgagcgagt ggtgcgcacg cgctcgatac cgaagcgatc cgccgcctcg tcgaagctgc
     5761 gcaggatctc attgagcaac tcgagcgcct tctccgactc catgttgcga ctgaacgcct
     5821 cgaagcccac gatgtcaccg aacatgacgg tgacctcctg gtgatcctgc gcgatggtgc
     5881 gcgccccctc gcggtacttg cgcaccaacg gctcgggcat aagcgagagc atgagccgct
     5941 cgttctcgtc ctgctgcgac ttgagcagcg cctccttcac ctggaggctg cggctcatgt
     6001 cgttgaacgc ggcgccgacg tcggcgagct cgtcgctcgt acccgcgtcg acctgcacgc
     6061 cggtctcccc gctcgcgatg cggcgtgcgg catcccgaag gcgacgcagc ggccgcacga
     6121 tgacgccggc gatcaccagg gagagcagcg acaccacgag cacgatgacg gccgacgaca
     6181 gcagcaggtt gcgggtgaac gcgtcgaccg gcgcgaaggc ctcggcgctg tcgatctcgg
     6241 cgacgatcac ccagtccagg ccgtcgatgt cgagcggggc gtacgcggcg atcgtctctc
     6301 cgcccaggta gccggccgcg agagtcgtac cggtgacgcc cgacgcagcg gatgccacag
     6361 catcggtccg caccggttgc agcaacagcg tgcttccgcc gtcgatcacc tgctgagcga
     6421 cgccgggtgc tgtccccgcc gcgactgcct gctccttgta ctggtcgggg ttctcgatca
     6481 ggtccctcga cagcgagcgc atgagcttgt cgtcggcgcc cacgatgtag gtctcgccgg
     6541 tgcgtccgag gccgctgccc gcccaatctc ccccgccggt catcacggca ttgatctcgt
     6601 cgatgggcat ctcgatcgcg agcgttccga tgatcgtgcc gttgaccgcg acgggcacga
     6661 cggcccatgc cgcgggcacg ccgagcgagg gcgggtacga cccgaagtcg gtgagcacca
     6721 cggtgtcggc gatgttgccg ttcatcgcct cggtgtatgc cttggccagg ttggtgcgct
     6781 gaagcggacc cgagtccagg ttgctcccga ggtcgacccc cttgaaggcc gagtagacga
     6841 tgttgccgtt ggcgtcgacg agcagcacgt cctcgtattt gaagagctgc gtcatccggc
     6901 ggaagaagtc gtggtactgc gcgtgagctg ccgaccaggt gctgccgtca ccggcatcgt
     6961 cgacggcgat ggcgtcagtg aaattgtcga acggcaccgt gtagtgcagc gtgagatagc
     7021 tctgggcggc gcccgtcgga atgaacgtcg acgcgtcgat gggctccccg tcacccgccg
     7081 cgccggaggc gctgagctgg gctccgaact gggtggtgaa gtagtcctcg aggctcgcct
     7141 gttcctcgtc ggtgagggtc gtcgactcga gttcctggaa cccggcggtg aacgcctgca
     7201 tcgcgttggc aacgctctgc ccgcgcgcgt tcaagagcag tgtgttctcc atcgaggtga
     7261 acagactcgt gatctcacgt gcccgggagt cgcgcacctc gatgaggctg ttcactgcgg
     7321 aatcccgcag cgagtcgcga ccgttcacat agccgatgag accgacgacg acactcgagg
     7381 caacgctgac cagcagaagc atgatgagca gcagcgaacg gatgctcagc cccgccttca
     7441 tcgtcgtgct gggcagcacg atcgaccctt cggcaggggg cggtgtcacg ccggcgttgg
     7501 acatggggct cctggatgcg gtgctgcggt gctgcgcgaa tcagtgctgt gcttggttca
     7561 ctttagtcac agcggccgca cccgcgacag tgcgcgacgg gcgcgaccgg cgcgcaccca
     7621 cgccgccagc gccgaccgca ctcgccagtc agtgcgtccc gaccgaccgt ggggttcttc
     7681 gccggactcc gctaactcag gacattagtt cgcagtctct acatacgtcc tgagttagcg
     7741 gaacaactgg gaagggaatg caagacgagc cacccaccgc gcgatgtcag cgttctcgcg
     7801 gcgagcgagc gtcaggacaa ccccggagcc caacggacgc taggcgccct gcacctgctg
     7861 ttcgtagagc gacgcgtaca tgccgccggc ggcgagcagc tcggggtgcg ttccgcgctc
     7921 cacaacgcga cccgcgacga cgacgaagat gacgtcggcg gccacgatcg tggagagccg
     7981 gtgcgcgacg gcgatcgtgg tgcggccacg ggacgccgca tccagcgcgg tctgcacgac
     8041 gcgctcggag agcgagtcga gggcgctggt cgcctcgtcg aggatgagca cggccgggtc
     8101 tttgagcagc acgcgcgcga tcgcgatgcg ctgcttctcg ccgccggaca gacggtagcc
     8161 gcgctcgccc accaccgtgt cgtagccatc ggggaatgag gcgatcgtgt cgtggatgtt
     8221 cgctgcgcgg gccgcggccg tgagctcctc gtcggtcgca cccggcttgg cgtagcgcag
     8281 gttctcggcg atggtcgcgt ggaagaggta ggtctcctgg ctgacgatcc cgatgttgcg
     8341 caccagcgaa tcctgttcga gcgcgcgcac gtcgtcgccc gcgaacagca cccgaccgct
     8401 cgtcacgtcg tgcaggcgcg ggatgaggta cgacacggtc gtcttgcccg ctccggacgg
     8461 tccgacgaag gccgcgaact gtccgggctc gatcgtgaac gacacgccct tgagggtcgc
     8521 gggcgagtcg gcgggcgcat ccgggtagcg gaagaccacg tcgtcgaact gcannnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgggcg agtcggcggg cgcatccggg
     8701 tagcggaaga ccacgtcgtc gaactgcact tcgccgagct tcttcgggcg caccggccgc
     8761 gcgtccggac ggtccacgat cgcgggcacg aggtcgaggt actcgaagat gcgggcgaac
     8821 agcgccgacg acgtctgcag gtcgagcgcg acccgcagca gcccgagcag cgggaacatg
     8881 agccgggcct gaacggtcgt gaacgccacg atcgtgccgg ccgtgacatc cgtgccaccg
     8941 ttgatgagcc acgctgcgac cagatagatg accgcgggga tgatcgacag gaaaatgttg
     9001 accatcgcga agaaccactg gccgctcatc tgctggctca cctgcagacg gatctggttc
     9061 tcgttctcgc gggtgtaccg cccgacctcg gcctgctgct ggttgaagct cttcgcgagc
     9121 aggatgcccg acacactcag cgcctcctgc gtgatcgccg tcatctcgct cagcgactcc
     9181 tgtgtcttgg tcgcaatgcg cgcgcgcacc tgaccgacct tgcgctgggc gatcaccagc
     9241 agcggcagca ggatgaccgc cacgaccgtg agctgccagc tgagcacgag catgctcacg
     9301 aacgcggcga tcacggtgac cgtgttgccg atgacgctcg agatggtgtt gctgagaacg
     9361 ccagccacac cgccgacatc gttctggagt cgcgattgga tgactccggt cttggtgcgc
     9421 gtgaagaacg cgagctccat cgcctgcagg tgaccgaaca ggcggatgcg cagggccccc
     9481 atcaccttgt tgcccaccgt ggccgtgagg taggtctgcc acacgccgag tgccgctgac
     9541 gagatccaga gcgccaccat cagcagcacc agttcgacca gcaccgggat gttgggtgtg
     9601 cccgacggcg ggaacagccc gacgtcgaac gcctgcttgg tgagcagggg cgggaacacg
     9661 ctgatcgcgg cgctcaccag cacgaggatg atcgtgacgg tgatcgccgc acggtggggg
     9721 gcgaacagct gcgagatgcg cttcaggagg tccggaatct tcggcgccgt cgcattggct
     9781 gcgcgttgag cctcgacgtc gccgccgctg atgcgacctc cgcgtccgcc gccgcgcatc
     9841 ccgctcatgg ttcaacccta agcgggttga gtagcgcgga gcgcgtatcg aaacctcacc
     9901 cgcgaggttt cgatacgccg actccgtcgg ctactcaacc ggcctgggct cagcccttga
     9961 cggatcccgc cagcaggccg cgaacgaagt agcgctgcag tgcgaagaac accgcgaccg
    10021 gcacgatgat cgagacgaat gcgccagccg tcagcaactc ccagtcgccg cctcgggttc
    10081 cggtcagctc cgccaatcgc accatcagcg gtgcgatctc acgtttacct ccaccgaagg
    10141 tcagaccgac tagcaggtcg ttccagaccc aaaggaactg gaagatcgcg aacgacgcga
    10201 tcgccggaat gctcagcggc agcaccacca tgcggaacgt cttgaacgca tcggcgccat
    10261 ccacgcgcgc cgcctccatc agttcccggg ggatctgcga gaggaagttg tgcagcagga
    10321 agatcgcgat cggcatggcg aacatcgtgt gcgctatcca gatcgggatg taggtgccgg
    10381 tgatgcccaa gggcgggatg agtgtggcat ccccgatctt cagtccgttg gcgaacagct
    10441 gcagcagcgg gattagggcc agctgcagcg gaatcacctg cattgcgaag atcgtgtaga
    10501 acagcaggtc gcgtcccttg aagtcgaacc aggccaacgc gtaggccgcg aaggtggcga
    10561 tgacgaccgg gaacaccgtc gcagggatgg tgatcgcgaa cgagttcagg aagtacaggc
    10621 tcagcggtgg cgtgaccgaa ctgccctcga agagaaccgt gttgtagttc tccagagtga
    10681 agctcgggtt ggtgaagaac gtccaccatc ccgtgttgtt gatgtcggcc ggcgtacgga
    10741 tgctcgagat gaacagaccg aacgtgggga tcgtccacac caacgcgatg actatcgcga
    10801 agaacgaggc gatgggcgag ccgaagatgt tgttctgctt acggctggtg agccgtatgc
    10861 ccttcgactt ggtgcgcgct ggggtgaggt tgacgggcgc gatttcctca atggcggtca
    10921 tcagtgaacc tccctctcct tgcgaacctg tcggacgttg aagatgatca gcggcagcac
    10981 tcccaggaac aggatcattg ccagcgcgga tccggttccg tagttgagcg cccggaaact
    11041 ctgctggtac atctcgttgg cgatgaccga ggtctggaag tttccgccgg tcatagtgcg
    11101 gatgatgtcg aagaccttga gcgatgtcac ggtgatcgtc gtgagaacca cgatcacgct
    11161 gccccagatc atcgggatcg tgatgcgcgt gaagcgcgcc catcccttgg ccccatcgag
    11221 cattgaggcc tcgatgatgt cgtcaggcac cgccttcatc gccgccgaga gcacaaccat
    11281 cgcgaatccg gtctggatcc agaccatgac ggcgatgagc aggaacgtgt tcagcggggc
    11341 ctcgagcagc cagttcggcg gggtcaagcc gatcgctttg aagatcgcac tcaggaggcc
    11401 gatgtccgcc ttgttggggt cgggctggta cgtgtagacg aacttccaga tgatggatgc
    11461 cccgacgaac gagatcgcca tcggcatgaa gatcagcgac ttgaccaggc cggcgtactt
    11521 catccggtcg gtgaggtagg cgatcgcgag tccgatggtc gtggatgcga tgggcaccac
    11581 gatcgtccag atcagggtgt tgcgcagcac gatgagaatc tcgggctgcg tgaacgccca
    11641 gatgtagttc tgcagcccga tgaagttctc actgcgggcg tcgaagaacg acaggtagag
    11701 ggtgcgaatc gccgggtaga tcaacccgat gatgagcaga agaagtgcgg gcccgaggaa
    11761 gacgaagtac ttcagacggt tctgagtctt ctcctttgtg cgctcggaga gcacgaagat
    11821 caggacgatg atggcgacga acaccaccag acccaccaga agcagaacga tgttgggcag
    11881 tactgtcgca aacggcgacc gggctgtcac aatccacatt gattgactca ttccgtaggc
    11941 agtagtgaga agaggtgcgg ggtccgcttg cgcgaacccc gcacagactt tcttatttag
    12001 tgcttagtag gacaacctac tacttgggcc agctcgcctc gatggcgtcg agaacggcct
    12061 tgtccgactt gtcctcggcg acccaggcgg tcatctcctt ccagaacgaa ccggcaccca
    12121 cagcagcggg catgaggtcc gaaccgtcga agcggaacgt ggtcgcgttc ttgaggatct
    12181 cgaccgcgtt cgcgttcacc gggtcagcca cgttggcggt gtccaggccg aggttggccg
    12241 agaaccagcc accgagggcg gccttcttgt tgttccactc cggcgtggtc aggtacagct
    12301 gaactgcctg aacctcggga cggtctgcga aggcaccgat gaactcgcca ccacccaggg
    12361 cgggcttgtc gtcagcggtc gcacccggga agtagaacgt ggtgatgcca ccctcagtcg
    12421 tggtgtcgtc cggggtgacg atggtcgcac cctggtcggc cacgaggatg ccgccgatga
    12481 aggaggcctg gcggtgcatg gcgcacgatc cgtcaacgat gccagcgcca ccggtctgga
    12541 agctcgtggt cgcgatggaa ggaacgtcac cgacgtactc cgggttcttc aggatctgac
    12601 cggccttgcc gatcgcctcg acgattgccg ggtcgttgaa cgggatctcg tgtgagaccc
    12661 acttgtcgta gacctcggcg ccaccgaagc ggaggacgat gtcctccatc cagtcggtac
    12721 cgacccaacc ggtggcgtca ccggactcga agcccgcgca ccacggctgc acgtcagacg
    12781 tggtcgcgat cttgtcggac agagccagca gctcgtccca cgtcgtgggg atgtcccagc
    12841 cgttgtcctt gaaggtcttc ggcgagtacc agacgaacga cttcacgttg gcgcccaggg
    12901 gcgaagcgta gaaggttccg tcgacggtgc cgtagttctt ccagtcgggg ctgtagttgg
    12961 cctcgacgct ggccgcgact gcgtcagacg ccggcagaag cttgccggtt gctgccatgg
    13021 cagcgagcag accaggctgc gggaagacgg cgaggtcggg agcagttcca ccctcgacac
    13081 gcacgggcag ctgggcttcg aactccttgg agccgttcca gttgatcgtg atgccggtgc
    13141 agtcctcgaa ctccgtgaag gactcctgga agagctcggc ctcggggctc acgacggtcg
    13201 tgtagatctc gacctcggta cccgagttgc cctggtagtc cgcgtaagcc tcacagcctg
    13261 cggggatgtc gcccgaaccg ctcggtgcgg agcatccagt gaggatgaga gcgaaagcgg
    13321 ttgcagaggt tgtcgccaga acgattgagc ggcgcttgat tgccctcatg gttaatctcc
    13381 tttgattgat aggggcggat cccgcgaaca agaatggctg tttctcgcgg aaaaccagct
    13441 taagactatg cgtgcaggtg ccctctgggg aacccctcac gtctcttttc cataacgcta
    13501 atcaaacttc ttcccggagt tggcccggaa tcgcctgtgg actgagagcg ctcccaacct
    13561 tcatttgtgc aaacgcttac atccgatgac gtgccctgag gcgccgatat cgcctgatcc
    13621 gtcacgaaag tgacgctcgt cgcgggctcg acggcgcgtg tcgcggagct gagatggagc
    13681 cggtaagcgc ccgaaacgcc caccggcccc atcgatcaat ggggcggttg aaccgccgga
    13741 gtggccacga tgcgaacaga atcgaccgac gagacgccag ccagcatcaa tggcgttgcg
    13801 gccagcattg tggtgatctg cgctctcggg tagcgagcgt tcgagtagtg ggctgggcgg
    13861 agggtaaggc cgtccagtcc gacgaacagc ttcgggtcac tctgtcgttc gaattgcact
    13921 gtcgttcgaa ttgcactgtc ggggtgtcac acaaatgggg gacaagcgtg cgacgagtcc
    13981 cattatgtgg ggcgaacgct cacgggtgca agacgtagct cggagcgagt gtcagagcaa
    14041 acaaaaaggg gcgccggccg cggccgaagc cacgggcgac gccccttgat gaaacgggac
    14101 tacttgaggg tgacggttgc gccggcctct tcgagcagtg ccttggcctt ctcggcggtc
    14161 tccttgttcg cgccctcgag cacgacaccg ggggcgccgt caacgagtgc cttcgcctcg
    14221 ccgagaccga ggctcgtgag ggtgcgcacc tccttgatga cctggatctt cttctcgcca
    14281 gccgactcga ggacgacgtc gaacgtgtcc ttctcctcga cctcttcggc tgcgccaccg
    14341 gcgccaccgg cggccggagc ggcagccacg gcgacgggtg cagcggcggt gacctcgaag
    14401 gtctcctcga acttcttgac gaactcgctc agctcaatga gcgtgagctc cttgaacgcc
    14461 tcgatcagct ggtcactggt cagctttgcc atgattttct ccttgttgtt tctgaacgat
    14521 ctgccgcgac ggtgcgcgac tgagtgtgtc gtccgcgtga actacgcgga ctgcttctcg
    14581 cgcagcgcgt cgaccgtgcg aacggcctgc gacagcggtg cgttgaacaa gtacgccgca
    14641 ccgaacagcg aggccttggc ggcacccgcg aacttcgcga gcagcacctc ccgggactcg
    14701 aggtcggcaa gcttcttgac ctcgtctgcg gtgagagggt taccgtcgag gtagccgttc
    14761 ttcacgagca gaagagggtt tgccttggca aagtcacgca gggtcttcgc gacggcgacg
    14821 gtgtcaccgt gcacgaaggc gattgcagac ggcccgacca ggtcatcgtc gaaagacgtg
    14881 atccctgccg cgttggccgc aatcttggtc agcgtgttct tcaccacggc gtatgtagcg
    14941 tgctcactga tcgacttgcg caggctcttg agctgcgcga ccgtgagccc gcgatactcg
    15001 gtgagcagta cagcgttcga ggtgcggaac ttgtccgtca actcggcgac tgatgcttcc
    15061 ttgttcgcca tggtgctccc tagatttcgt atggtgtctg ccggaggaca cccggagcaa
    15121 tgaaaaaagc tccgagccag gggcacggag ctgaacacgg cgcaaaccga tgaactactt
    15181 cacacacctg cgctggcctc gctgtgaagc gaactcagtc gaggtgcgat tgcgcgcacc
    15241 acgaaacctg cggtctttgg cttctcccag agtacgggat gccgcgacat gccgcaaacg
    15301 cggcagatcg tgagggcggc agacgaaagg caccgcacgg catgatggtc aggtggacag
    15361 tgcctttccc gtcgtcttcg tgcacggcct gttcgggtcg ttcgctgaac cagcggcgac
    15421 acgccacctc ggcccggcgg agttctcggc tcccgacctg cgtgggtacg ggcatgagac
    15481 ggaggtcgac gtgtcgttgg aggggcaggt cgcggcactt cgctcgcacg tggagtcgcg
    15541 gcatccggaa accccagtgc acctggtcgc gcactcgatc ggtgccgtgt atgccttcgc
    15601 cctcgccgac gaatcgcccg acctcgtcga gtctgtgaca accgtcgagg gcaacttcac
    15661 tctcgccgac gccttctggt cacggtcgat cgcggctctg gatgaagatc gggcacgttc
    15721 ggagatcgag ggccggttgc gtgatcctcg cgcgttcctc gctggcgacg gaattgaggc
    15781 cactgacgag cacctcgcca aggcggtcga cgcgctcgcg taccagccgt ggcgcacggt
    15841 gtggatgtcg gcgtcggcga tcgtcgaagc cacatcgaaa ccggagtacc aggagttgct
    15901 gcggcgcgtg ttcgcccgcc accgggtgca tctcgtggcg ggtgagcgca gcgcgggcgg
    15961 ctgggatgtg ccgggctggg ctcgcgaggc ggccgcgtca tccacggtca ttcctggcgt
    16021 gggtcacatg atgatgctcg agaatccgga tgccgtgggc cgggtgctcg ggcaactgct
    16081 gttcaccccc gaggggcgtc gcacgcccta ggcttcgggc ggcgtcgccg ggcgccgcgg
    16141 agcgcgcggc ttcttcggtg gagcaggcgt ggactccgcg tcggcggtgg gctcggctgg
    16201 agcctcggtc gccgcctcgg gcgcggattc gatggaaggc ttggacttgg cggggctccg
    16261 cggtgcgcgc ggcttcttcg gtgccggcgg ttcgggcgtg ggctcgggtt cgggctcggg
    16321 cgtgggcgtg ggctccgcct gagccgcgac gccgccccgc cgattggcgc gacgggcgag
    16381 cagctgggga accagaagga tgacgacgac accgaccaac gccacgatga tgaccgcgac
    16441 cggcgtgatc agactggagt tctccggcgc atccaccggc tcgcccgatc ggtcgatcat
    16501 catccgctgg aagtcgtcga gcggaatcgg cccgctcagg cctgacgtgc cggcgacgag
    16561 cgggatgagc tcgtccgcgc cgagggtgaa ccaggcggcg cgctgctcgt cgcgcaccac
    16621 gtaggagtcg gcaggcacat ccgagagggc cctggccagg tcggggtcgc gcacgaaatc
    16681 ggcgagctcg ggtgcaaccg acgcggggtt gatccagatg atggcgagcc cgatggtgtc
    16741 gtcgttgacg gtgatcggtg ctgtccacag gttctgccgc tcgaccgggg tgtcggtggc
    16801 gaccccggcc acgaacgcct cggtgaaggt gaagacccgg ttgagctggc cgaccgccgt
    16861 ggtctcgttg aagtcgatgc cagcgccacc ggagccgatg ccgaacaggt catcgaggcg
    16921 ggacaccagg ccgttggggt ctgcggcgta cgcggagacc tcggcgggca ccgatccctg
    16981 cgacgctgtg gcggccggcg cgccgacgag cgcgagtgcg aaagcggcga gcagagtcag
    17041 ggccgcgccg attcgggcgc gagagtgact gcgacgacgg gtgtgttcgg gcacccgctc
    17101 agtctcccac gacaccggcg aacgggccgg atgccgatca ggcgatttca ccgatcggca
    17161 ggatgatgcg aaacaccgtg ctgcccggcg cactctccac ctcggcacgc ccgccgtggg
    17221 cctcaacgac cgccgtgacg atcgcgagcc ccaggccggt gctgcccgcg gcccgtgagc
    17281 gcgaaccgtc gccgcgcacg aaccgctcga agatggtcgg ggtgagcgcc gggtcgatgc
    17341 cggggccggt gtcgcgcacc tcgatggtga cccagccatc gccgcggatc agagccgccg
    17401 tgacgaccgt accggccggg gtgtgggtac gcgcgttggc gagcaggttg gcgacgacct
    17461 ggtggagcct cggctggtcg ccgtgcacct gcaccgcttc atccggaagt tcgagttgcc
    17521 agatgtggtc cggtccggcc gcgcgcgcgt cgctgagtgc gtcgatgacc agccgcgtga
    17581 ggtcgaccgt cgcgccctcg attgccgcgc cctcgtcgag acgggccagc agcagcaggt
    17641 cttcgacgag cgcggtcatg cgcaccgact ccgactcgac tcggccgatc gcatgcacca
    17701 catcgtcggg cagctcgtgg cggccgcgcc gggtgagttc ggcgtagccg cggatcgacg
    17761 ccagcggcgt gcgcaactcg tggctcgcat cggcgacgaa ggtgcgcacc ttgttctcgc
    17821 tggcttgacg tgccgtgagg gcggatgcca cgtgctcgag aagagtgttg agcgcggcgc
    17881 cgacctgccc gacctcggtg ccggggtcgg cgtcctgggc gggtacgcgc tcggcgagcg
    17941 ccacctcacc gcggtcgagt gggagctcgg cgacgcgggt cgctgtcgac gcgacccgct
    18001 cgagtggacg cagcgcgagt cgcacgacga cggcgccggc gatcgctgcg gcggcaacgc
    18061 ccaggattcc gatcaccgcg atgaggagcg tgaggcgcag cacgatggct tgcacctcgg
    18121 agagcggaag accgatgagc agcgagacgc cgctggcgga ggtcttctgc atgatgaccc
    18181 ggtactggcc gagtgtgccc ccgagatcga cggtgacggg tgcggcaccg ggctgcacgg
    18241 tgccgaggga ggcgagttgc gtggcgtcga ggctgatggg cacgccgctc tggtcgagca
    18301 ctgcggccgt ggtcagctga ttggatgcga cgagccccgc tagggtgccc gcaccctggc
    18361 cgggcagcgc gagcacctcg cctgccggcg gtcggccccc gaagtcgtcg ttgggacgcc
    18421 cctcgaacgc ctcctgagac cgcccggtcg cggccgtcaa ctgcgcgtcg agccgcccga
    18481 cgagaaatcc ctgcagcgcg aacacgctga agaccccgat cagcacggtg accagggcga
    18541 gaagggcgac gatgccgaaa accaggcgcc gacgcagggt gaggcgacgc gtcatccggc
    18601 cggcttcaga acgtagccga accctcgcac cgtgtggatc atgggttcgc cgagcgtgtc
    18661 gatcttcttg cgcaggtacg agatgtagat ctcgacgacg ctggagcggc caccgaagtc
    18721 gtagctccac acgcggtcga ggatctgggc cttgctgagc acgcgtcgcg gattgcgcat
    18781 gaggaagcgc agcaactcga actcggtcgc ggtgagctcg atggcggcgc cagcgcggct
    18841 cacctcgtag ctgtcctcat cgagtcgcag gtcgcccacc tcgagcacgg agtcggccga
    18901 gtcggagacg gcgagcgccg agcggcggat gagcccccgg agccgggcga cgagctcctc
    18961 gaggctgaac ggcttggtga cgtagtcgtc gccgccgacg gtgaggccgg cgacacggtc
    19021 atccacggag tccttggcgg tgaggaagag gatgggcacc tggatgccgt cggcgcgcaa
    19081 ccgcgacagc acggtgagcc cgtcgatgtc ggggagcatg atgtcgagca cgaccacgtc
    19141 gggccggaac tcgcggatga ccgacagcgc cttctggccg tcgctggcga gcttgacgtc
    19201 ccacccctcg taccgcaggg ccatcgagag caggtcggtg agcgaatcct cgtcgtcgac
    19261 cacgacggca cggatcgggc tgccgtcggc gcggctgatg cggggctgtg ccgcaatcgc
    19321 gggcgcgtgg ttggaggtcg tcatgtctcc cagtattcgg agattcctat gagccgcctg
    19381 tgaagtagcg tccttggctg tttctcaggt tcggcatagg gcgccgatag cgacgatccc
    19441 taggtttcgg atgtccattg cagacacaca aggagaccca tgttcggcac ctacctcttc
    19501 cgggagctca ccaaccggcg aaagcagacg gcgatcatcg ccatcggcat ggccctggcc
    19561 atcgccctcg tcatcatcgt caacggcgtc tcggcaggcg tgagcgccgc ccaggcgaag
    19621 gtactggagt ccgtgtacgg cgtcggcacc gacatcacgg tctcgcagca gccgacggga
    19681 cccaccgagg gcggggccgc cggcgggccg ccctcgttcg acttcggatc gggcgacgga
    19741 tccacagcag acggctcgac tcagctcagc caggcccgac tgacgaccgg acccggttcg
    19801 gcgacctttg ctgacaccgc cctggccacg gtgcagggcg tcgacaacgt gagctccgcc
    19861 acggccacgc tgagcctcac gaacatcaac ttcagcgggg agatccccga cttccaggcc
    19921 ggcggtggtg gcctgccgac tgacggcacc gcgccgccca cgggtggagc tgacggtgct
    19981 ggtggcagct cgttcgacat cgagtcgatc actgtgacgg gcatcgatgt cacaggcgat
    20041 gcggtcggcc cgctcaccgc ggtgagcctc accgacggcc gttcgttcga tgctggcgat
    20101 gtcggcacca acgtcgtagt gctcgacagt tcctacgcga ccaccgctga actcggcgtg
    20161 ggcgacaccc tgggtctcgg gggcgaggac ttcgaggtca tcggcatcgt cgcatccacc
    20221 tcgtcggatg ccagctcgac caacacctac atcccgcttg acacggcgca
//
LOCUS       DAOSOU010000009        20070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2686_length_20070_cov_7.561629, whole genome shotgun sequence.
ACCESSION   DAOSOU010000009 DAOSOU010000000
VERSION     DAOSOU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 20070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20070
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2686_length_20070_cov_7.561629"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..73)
                     /locus_tag="PLW15_01005"
     tRNA            complement(2..73)
                     /locus_tag="PLW15_01005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Arg,seq:cct)
     gene            153..920
                     /locus_tag="PLW15_01010"
     CDS             153..920
                     /locus_tag="PLW15_01010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT33009.1"
                     /translation="MALYSSYIAIGDSFSEGYGDEGPDGVPRGWADFVALGLALASAP
                     ATVTYANLAIRGRKLAPIVGEQLDAALAQHPQLLSLNGGGNDLMRPRVSIGHIAEQLD
                     AAVDRAVAAGTHVVLASGANPSRHIPLGGVLARRGEELARAVRQRLPRPGVTFVDNWG
                     DTELAELRYWSRDQLHLNALGHARVAGNVLAALEVAIPEFGAGELSAPRPRTSAYWRE
                     YVLPWIGRRLTGRSSGDNRDPKIAVLSPVEIGQDGGG"
     gene            complement(902..>1697)
                     /locus_tag="PLW15_01015"
     CDS             complement(902..>1697)
                     /locus_tag="PLW15_01015"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HOT33010.1"
                     /translation="QRQQDRQPLQQGRKQRKLKASERRALQASAPPQQRKNPLNVLAT
                     MAVVGGMFAVAGLPAYALQTPENTAATKSDASAQSVVVSSDASLATPTRDGYHATTYE
                     QLAQMEDDAQREANQAAYLASGARALGDDYPWPYELRDYQGGGLSPLNYYYRECVDFV
                     AWRLNRDAGYYAAPYKWVWANLTPSGGNASQWRYNWEQHGWPVSTTPIVGSVAWFYGN
                     HVAYVKSVNADGTVTIEEYNYSSNGTYGQRTIPASSVPAFLYPPPS"
     assembly_gap    1698..1797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1951..2448)
                     /locus_tag="PLW15_01020"
     CDS             complement(1951..2448)
                     /locus_tag="PLW15_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT33011.1"
                     /translation="MRTLVLNAGYEPLAVVSFKRALVLVMNQKATVIAADAENPVWGS
                     TGAWDRPSVIILRNYVRVPSGRHVPVSRRGVLRRDNHRCAYCGSTANTIDHVLPRSRG
                     GKDSWENLVACCLRCNNIKGDRTPSEMSWTLRSIPRPPRGTSWLVRGIERALPDWEEY
                     LAPAA"
     gene            complement(2750..3322)
                     /locus_tag="PLW15_01025"
     CDS             complement(2750..3322)
                     /locus_tag="PLW15_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HOT33012.1"
                     /translation="MYTHRTLAVVGVLAASLALSACAAPASDMGMGANSPSEQSTDTN
                     QADTSFTMMMIPHHQQAIEMSDTILAKANIDPRVQELAQQIKDAQAPEIELMQGWLDD
                     WGFGSMSGMDHGGGMMSDDDMAALDSATGVEASRLFLTQMIEHHQGAIDMAKEELDNG
                     VNADVKALCDRIISSQAAEIETMKTILATL"
     gene            complement(3388..3810)
                     /locus_tag="PLW15_01030"
     CDS             complement(3388..3810)
                     /locus_tag="PLW15_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33013.1"
                     /translation="MDVMPRALRCVIIAALVVSALVLGAASIHAMTSGSSSMGAMSAT
                     THVMAPADDMSGMHHSATAAATHPTCAAGMCDAGHALVTILCVAGVMALVLLLLLLLP
                     RPGDASRMFRVIEERGSSGALARPRHTPGLTQLSVSRT"
     gene            complement(3845..4618)
                     /locus_tag="PLW15_01035"
     CDS             complement(3845..4618)
                     /locus_tag="PLW15_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOT33014.1"
                     /translation="MTKVGPSKTSSTAVEPGSVDSVGSEVPAAFRAKRPSSNRKSSIL
                     SLAVMALIVPGIFATVALPAYAFSPAPDTSGVEASQALQEITQRDAQTVVVAAKAEVA
                     TVSRDGFTTTSAAEMARAALAVEYSAYTGPSAADFLANPPYPNFSLDQVVSVALQYTG
                     VPYRYGGADPSGFDCSGYVMFVYAQFGIALPHSAAGIGASGTPIATSDALPGDIVIMP
                     GHTGIYMGDGNFIDSGDYGDLIHVRPIYDSNYYIVRVGI"
     gene            complement(4831..5523)
                     /locus_tag="PLW15_01040"
     CDS             complement(4831..5523)
                     /locus_tag="PLW15_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT33015.1"
                     /translation="MTDLVDTTEMYLRTILDLEEEHIVPLRARISERLGHSGPTVSQT
                     IGRMERDGLVVVEGDRHLELTPEGRNRAIHVMRKHRLAERLLADVIGLDWAYVHDEAC
                     RWEHVMSEQVERRLLEMLGHPTESPYGNPIPGLAELGDAPAPAFMDGVINLVDVVAAT
                     NEPVSGVIRRLGEPVQFEPELLQQLQNAGVMPGALATISAAGSYVFVQVEGFGEGLEL
                     PNEVAVHIFVAR"
     gene            5564..6670
                     /gene="serC"
                     /locus_tag="PLW15_01045"
     CDS             5564..6670
                     /gene="serC"
                     /locus_tag="PLW15_01045"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOT33016.1"
                     /translation="MPSLVIPSSLLPADGRFGCGPSKIRQEQLDFLVANADLLGTSHR
                     QAPIKDLVGRVRAGLGELFRIPDGYEVVLGNGGSTAFWDAAAYSLIDQRSENLTFGEF
                     GAKFAQAAGAPHLTAPHVVSAPAGSRASVEIVDGIDVYAWPQNETSTGVMAPVTRVDA
                     PGALTVIDATSAAGGIDFDSSQADVYYFAPQKNFASDGGLWFALFSPAAIARVEAIAA
                     SDRYIPEFLSLKNAIDNSRLNQTLNTPAISTLLLLQNQVDWINGNGGLGWASARTAES
                     SGALYEWADASSYATPFVTDPAHRSQVVVTIDFDESVDAAAVAAALRANGVVDTDPYR
                     KLGRNQLRVATFVAIEPDDVRALIGSIEYVVEHL"
     gene            6735..6968
                     /locus_tag="PLW15_01050"
     CDS             6735..6968
                     /locus_tag="PLW15_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HOT33017.1"
                     /translation="MRLWLKDSERRPDPAPVKTDDRKPMLIGILVWLVALAVLLFFIA
                     PLTAAHNEWWLWTCIAGIALGLVGILYTNWRHK"
     gene            complement(6973..7542)
                     /locus_tag="PLW15_01055"
     CDS             complement(6973..7542)
                     /locus_tag="PLW15_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HOT33018.1"
                     /translation="MPESYEDLARSALLEITPVATIGSFVSQTPEDDGSVTVRFASAQ
                     PGYPDWLWTVSVADVEGGEPTVLEAELLPGEGSLLAPDWLPWSDRLAEYKAAQEALGE
                     AAVEDGDADDDDPDDEDDVADDESDDEDDDLDDHVMDDDDVLGSDVLHGGDLDGVDID
                     DPDEDLDDDDESDDDDDDDDESDEGERSF"
     gene            complement(7535..7918)
                     /locus_tag="PLW15_01060"
     CDS             complement(7535..7918)
                     /locus_tag="PLW15_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT33019.1"
                     /translation="MPTGKVKFYDDEKGFGFISSDDGQEVFLHASALPTGTTGIKAGT
                     KLEFGIADGKKGAQALSVRVLEAPPSMAKLNRKSADDMSIIIEDLVKLMDGIGAGLKR
                     GRYPDKSHSSKIAAMLRKVADDLDA"
     gene            7979..8245
                     /locus_tag="PLW15_01065"
     CDS             7979..8245
                     /locus_tag="PLW15_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33020.1"
                     /translation="MANQTPITDHRAERVLAFMVASAIGLSIIAFLAVIIGTATGVTD
                     FSGGVWPVAIVLPAIGLPIGLVLMIALIIVSGLRRGREARDASK"
     gene            8232..10079
                     /locus_tag="PLW15_01070"
     CDS             8232..10079
                     /locus_tag="PLW15_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOT33021.1"
                     /translation="MPASNDSALVLAGQLRSLSDAALAELLTAREIRGTGIRDFFDLA
                     DALLDPAAIQHALGRLDRSTLATLAALGELGPVTEADAAAHLTTLGADAALLPERLAT
                     AARLALLTQHLGRWVVPASVAEQLQLWPSLGLPSLHELVSLSAPAALSPVSGVDVKFT
                     DHVASENAFQTTTSIAELIATLDLESARELARGGIALPDSKRLAAAMGVDLDRVSGLL
                     DIASRAGLVALDSGRWMPAEASRAWMVDSSGERWALLATAWLERLPVDIRTILRERAH
                     AVWGERLEEYVAWLFPAGDDWMRDRVRVYTRDAELLGITADNVPSTPGAALLASGADA
                     AATAMAALFPPEVAQVYLQHDLSIVSPGPLAPPIDARLRTMADVEGRALASTYRVSTA
                     SLSRAMAAGETESSMREFLGGIALTGIPQPLDYLLTEAASRYGLVRVGATPEGRTYVS
                     STDGTLLRTLVVDHSIGSLGLVRSGDRLESRYDRDLVFWALSEARYPVAAENAAGEIV
                     TLARRQASRITAAAATDTTGALIERLRVGSSADPDVTGKAWLARQLDVAIKGKVGLTV
                     TVRMPDGSDVDYQLEPASVGGGRLRARDRRSDIERTLPLTSIVAVAPAE"
     assembly_gap    10105..10204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10283..11926
                     /locus_tag="PLW15_01075"
     CDS             10283..11926
                     /locus_tag="PLW15_01075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT33022.1"
                     /translation="MPTNGPLIVQSDRTVLLEVAHPDAEDARHDLAVFAELERAPEHI
                     HTYRITRLGLWNARAAGHTAADMLATLEKYSKFAIPQSVVVDMTETVGRYGRLVIERA
                     DDGALTLTATDAAVLSEITRARKVAALLFEKRGDHTYVVQAWARGQLKQELLKLGWPA
                     EDLAGYTPGTPHEIALDESAWHLRPYQHQAIDNFAAHGSGVVVLPCGAGKTLVGLGAM
                     AAGKTTTLILVTNTVSARQWKAELLKRTTLTEEEIGEYSGQVKQVRPVTIATYQILTA
                     KRQGEYAHLALLDALDWGLVIYDEVHLLPAPVFKLTAELQARRRLGLTATLVREDGRE
                     GDVFSLIGPKRFDAPWKEIEAQGFISPASCFEVRIDLPENERLEYAASADDERYRLAA
                     TAPAKLGVVRELVAKHAGERILVIGQYLDQIDELADALGAPKLTGATPIDERERLYQE
                     FRDGVTKVLVVSKVANFSVDLPEATVAIQVSGSFGSRQEEAQRLGRLLRPKESGLPAN
                     FYTLVARDTVDQDFAQNRQRFLAEQGYSYTILDASSLVE"
     gene            12045..13115
                     /locus_tag="PLW15_01080"
     CDS             12045..13115
                     /locus_tag="PLW15_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HOT33023.1"
                     /translation="MTVTPFVIDVPQSDLDDLRARLRATRWPDAAVSDWSQGIPPAAL
                     RRVLDYWAEEYDWRAVERQLNTLTHVTVDGIHAVRAGTPGATPLLLVHGWPDGFVRFL
                     RALPLLADRFELVIPSIPGYGFSAQPAEPLGPIGVADRFVDLMSQLGHERFAVHGGDI
                     GTQIADQLGLRHPDRITALHLGDVPLRRLRGIDPAEQTPEEQQWSKDGAAWELAEGAY
                     SHLQRTKPQTLAAALNDSPAGLAAWILEKFEAWSDKGLDAFTLDDLATNLTIYWVTQT
                     AGSAARYYYDNTVTPTGRQVVPTGFAVFPAYICPPPPGSASRWYPVERWTQMPRGGHF
                     GPWEEPELWAAEVRAFFDQLEL"
     gene            complement(13204..13938)
                     /locus_tag="PLW15_01085"
     CDS             complement(13204..13938)
                     /locus_tag="PLW15_01085"
                     /inference="COORDINATES: protein motif:HMM:NF010663.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HOT33024.1"
                     /translation="MSAAIDMLWPGAPRGLSDDALIDSLAVAAPTVRANFVASIDGAA
                     THDGRSGGLSGAADKRHFELLRRVTDVVLVGAGTVRDEGYGPMLVDEASQLWRERHGM
                     PRHPVFAIVSGHLDLDPTSPIFTEAPVRPIVITTAGTDTTAFAAVAHVVAVGDEHVDL
                     AAALAALRAMGHAQVLCEGGPSLFAAMLAADLIDELCLTVAPTLEGGDGPRIVGGDIP
                     ARSMRLVHVLRGGEELLLRYARTLVE"
     gene            complement(13935..14807)
                     /gene="folP"
                     /locus_tag="PLW15_01090"
     CDS             complement(13935..14807)
                     /gene="folP"
                     /locus_tag="PLW15_01090"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT33025.1"
                     /translation="MPRIGQRDFDFDRQIAVMAVINRTPDSFYDRGATFELDRAIGAA
                     LTAASEGADWVDVGGVPFGRGPAVTVAEEIARVVPVVEGIAAASDVVISVDTNNAEVA
                     RAAIAAGAAVINDTSGLGDPDMAAVIAESSAHVVITHSVGPPRVEKPAAHFDDVVREV
                     VDFLRARVDRALQAGVPAERIIIDPGHDLDKNTLHSLELTRRLEEVVALGYPTLVAVS
                     NKDFIGEVLDRERTERLEGSLAAMVVCIMKGARIVRMHNVAASVAAVRMTEAILGFRE
                     PAYLLHNTHPTLNV"
     gene            complement(14845..15969)
                     /locus_tag="PLW15_01095"
     CDS             complement(14845..15969)
                     /locus_tag="PLW15_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33026.1"
                     /translation="MRRPALVILALPLALVGALLAAAPAQAASQPLFGTTVDVTLQPS
                     NDSLLGGTAYALSSGGGVQVTLGAALAADVDFELYEGATLIASCTVATGFTSCTDGVS
                     ALTAGAHSVTARFTQSAVTVDYTGTIFTVTNVAPTVLLEWQDASGAWIDGSFAGVPFF
                     GTTAARCVVTNNSNAAFTFASSFTGTVSHTGGPTITAITGDLAAGATGYYPIWSGAVS
                     LSPAASCGGGVDFPDGSGNGGGTGGGVIPVSGTIEASPAPAPGRTVTITAAGLLPPVA
                     SAYYVTVDGVDVAGPFTTPAPDFAFSADVALPANLAAGTHSVAVYAVFDERKIVFAYF
                     PFEVALAATGVDATAPVIAGSIALALGTLLLLAARRRRTV"
     gene            16084..16776
                     /locus_tag="PLW15_01100"
     CDS             16084..16776
                     /locus_tag="PLW15_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33027.1"
                     /translation="MADGPKILIVDDEPNIRDLLTTSLRFAGFAVRAVGNGAQAISAV
                     LEEEPDLIILDVMLPDMNGFGVTKRLRASGYTAPILFLTAKDDTEDKITGLTVGGDDY
                     VTKPFSLDEIVARIKAILRRTMHDDEEAIIRAGDLTMDQDTHEVTIGTEQVELSPTEF
                     KLLRYLMLNPNRVLSKAQILDHVWEYDFNGDAGIVESYISYLRRKLDQYSEEPVIQTK
                     RGFGYMLKASKV"
     gene            16808..18475
                     /locus_tag="PLW15_01105"
     CDS             16808..18475
                     /locus_tag="PLW15_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT33028.1"
                     /translation="MHEIVEWWNRVSLRTKITGVTVLLLTAGLVIAGVGTMAVLRGYL
                     QDEVDTKITAAGNKLAGNILFAGTADNPQCSVSTLPNDYYLGVLDYNGVLLCNNREED
                     APRPVLDTLTLGAVAGSEGAFTLPSTALGGSWRVIAYPATNRLGTDYYTVVIGLDLKD
                     TNGIVTRYAAIFLFFGLTVVILGGALTRLLVTTTFAPLREVEATAARFAGGDFSQRLS
                     GATPNTEVGRLNRSLNSMLSRIDRAFDERAKTIDQMRRFVGDASHELRTPLVTVRGYA
                     ELYRMGALTKQEDVAQAMERIEKEAIRMGELVEDLLELARIDEAKPLQLTEVDLVPLA
                     QDAALDTMASAPNRVVTVVGTDATTEEPPAETAVLPVVAPTPAQQRGGLATGPIAFAG
                     ATLARLTARRTRANRSGGDEVTIAEPPPAELRAVVLAEENKIRQVLSNLIGNALRFTP
                     ESSPIEIGVGVDAARQRAILSVIDHGEGIPPQIRGKIFQRFWRADTSRTRDTGGSGLG
                     LAIVAAIIQAHQGDVEVVETPGGGATFRVSLPLLPSTPDAAASPSRA"
     gene            18515..18805
                     /locus_tag="PLW15_01110"
     CDS             18515..18805
                     /locus_tag="PLW15_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HOT33029.1"
                     /translation="MTRYQVDSEAVLQATGAVRTSIGRIQAEVAGLHGQLSALEGSWT
                     GAAATAFSGVVTDWKATQQQVEQNLAAINHALTQAGQQYADIESANTRLFAR"
     gene            complement(18978..>20070)
                     /gene="groEL"
                     /locus_tag="PLW15_01115"
     CDS             complement(18978..>20070)
                     /gene="groEL"
                     /locus_tag="PLW15_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT33030.1"
                     /translation="TFGTELELTEGMRFDKGFLSAYFVTDQERQEAVFEDPYILIANS
                     KISNIKDLLPIVDKVIQSGKQLLIIAEDVDGEALATLVVNKIRGIFKSVAVKAPGFGD
                     RRKAQLQDIAILTGGQVISEEVGLKLENATLDLLGKARKVIITKDETTIIEGSGDEDA
                     IAGRVKQIRAEIENTDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDA
                     VRNAKAAVEEGIVAGGGVALIQAGKIAFDGKALKALLGDEATGANIVRVAIDAPLKQI
                     ALNAGLEPGVVADKVRHLPAGQGLNAATGEYVDMLAAGINDPVKVTRSALLNAASIAG
                     LFLTTEAVVADKPEKSAPPMGDPSGGMDF"
BASE COUNT         3252 a         6868 c         6566 g         3184 t
ORIGIN      
        1 gtgcccctgg agggactcga acccccaacc ctttccttag gacggaactg ctcttccatt
       61 gagctacaga ggcttggctg caccagttta tcggtggctc gcgccgacag cccgtgcacc
      121 gggcccggcg cccgatgtgg tgagatcgaa ccatggcgct ctactccagt tacatcgcga
      181 tcggcgacag cttcagcgag ggctacggcg acgagggtcc cgacggcgtg ccgcgcggat
      241 gggccgactt cgtggcgttg ggtctcgcgc tcgcgagtgc gcccgcgacc gtcacctacg
      301 cgaacctcgc catccgtggc cgcaagctcg cacccatcgt cggtgagcaa ctggatgccg
      361 ccctcgccca gcacccgcag ctgctcagtc tcaacggcgg aggcaacgac ctcatgaggc
      421 cacgcgtcag catcggacac atcgccgagc aactcgatgc cgccgtcgac cgcgcggtgg
      481 ctgccggaac ccacgtcgtg ctggcgagcg gcgcgaaccc gagccggcac atcccgctcg
      541 gcggcgtgct ggcgcgccgg ggtgaggagc tcgcgcgcgc ggtgcggcag cgcctccccc
      601 gccccggcgt gaccttcgtc gacaactggg gcgacactga gctggccgag ctgaggtact
      661 ggtcgcgcga ccaactgcac ctcaacgcac tgggccacgc tcgggttgcc ggcaacgtgc
      721 ttgcggcgct cgaggtcgcg atccccgagt tcggcgcggg cgagctgtcc gccccgcgac
      781 ccaggacgtc ggcctactgg cgcgagtacg tgctgccctg gattggccga cggctcacgg
      841 gacgatcatc cggcgacaat cgcgacccca agattgccgt gctgagccct gtcgagatag
      901 gtcaggacgg cggcgggtag aggaacgcgg gaaccgacga tgccgggatc gtgcgctggc
      961 cgtaggtgcc gttgctcgag tagttgtact cctcgatcgt gacagtgccg tcggcattga
     1021 ccgacttcac gtacgcgacg tggttgccgt agaaccacgc gacggatccc acaatcgggg
     1081 tggtgctgac cggccagccg tgctgctccc agttgtagcg ccactggctc gcgttgccgc
     1141 ccgacggggt gaggttcgcc catacccact tgtacggtgc cgcgtagtat ccggcgtcgc
     1201 ggttgaggcg ccacgccacg aagtcgacac actcgcggta gtagtagttg agggggctga
     1261 gcccgccgcc ctggtagtcc cgcagttcat acggccacgg gtagtcgtcg ccgagcgccc
     1321 gcgctcccga ggccaggtag gccgcctggt tggcttcacg ctgggcgtcg tcctccatct
     1381 gggcgagctg ctcgtacgtc gtcgcgtgat acccgtcacg ggtcggcgtg gcgaggctcg
     1441 catcggagct gacgacgacg ctctgcgccg acgcgtccga cttggtggct gccgtgttct
     1501 cgggcgtctg caaggcgtac gccggtaggc ccgccacggc gaacatcccg cccacgacgg
     1561 ccatggtcgc cagcacattg agcgggttct tgcgctgctg cgggggcgcg gatgcctgca
     1621 gcgcccgccg ctcggacgcc ttcagcttgc gctgcttgcg cccctgctgg aggggctgtc
     1681 ggtcctgctg ccgctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa
     1801 ttcgagggtg caccgaagtt gaacaggtca tccatgtcac tggatggctg aagatcaacc
     1861 acgaacgaac ttcccttcgg gcaaacggga cctcttcgag ggtacgggac taccccccgg
     1921 cctgtcgaat tgtcacagca acataacagt tcacgcagcg ggggccaggt actcctccca
     1981 gtccggcagc gcgcgctcga tcccccgcac gagccagctc gtgccgcgag gcgggcgcgg
     2041 gatgctgcgc aaggtccagc tcatctcgct cggagtgcga tcgcccttga tgttgttgca
     2101 gcgcaggcag caggcgacga ggttctccca gctgtccttg ccaccgcggg agcggggaag
     2161 cacgtggtcg atggtgttgg cggtcgaccc gcagtaggcg cagcggtgat tgtcacggcg
     2221 aagcacaccc cgacgcgaca ccggcacgtg acgaccgctg ggcacccgca cgtagttacg
     2281 caggatgatc accgaggggc gatcccaggc tcccgtcgat ccccacaccg gattctccgc
     2341 gtcggcagca atgactgtcg ccttctggtt catcacgagc acgagggctc gcttgaagct
     2401 cacgacggcg aggggctcat acccagcatt caggaccaag gtgcgcacgt tcatcctctc
     2461 gaaagagcct gcacggcttg caggcgttcg attcctggag gatcccgccc gacgaagggg
     2521 aggcgcccaa acaaaaaggc actgtcagaa gacagtgcct aatgttcgcg agcgtgagtg
     2581 atcacgctcg cgggactgtg ccctatgaag aactgagcgc gcgcatccac ggtttggatg
     2641 ctgcgcctgc ttttcatttg gcactccgta gttcgaagaa actccaagaa ctacaggcta
     2701 acccagagtc ggcgcgcctg ttttcactgg cgcgccgacc ggtcgtgaat cagagtgtcg
     2761 cgaggatcgt cttcatcgtc tcgatctcgg cggcctgcga tgagatgatg cgatcacaca
     2821 gagccttgac gtcagcgttg actccgttgt cgagttcctc cttcgccatg tcgatcgcgc
     2881 cctggtggtg ctcgatcatc tgcgtaagga agagccggga tgcctcgacc cccgtcgccg
     2941 agtcgagcgc tgccatgtcg tcgtctgaca tcatgccgcc gccgtgatcc atgcccgaca
     3001 tcgagccgaa cccccagtca tcgagccagc cttgcatcaa ctcgatctcg ggtgcctgag
     3061 catccttgat ctgctgcgcc aactcctgca cgcggggatc gatattggcc ttggcgagga
     3121 tggtgtcaga catctcgatc gcctgctggt ggtgcgggat catcatcatg gtgaatgacg
     3181 tatccgcctg gttcgtatcg gtggactgct ctgacggcga gttcgccccc atccccatgt
     3241 ccgacgccgg agcggcgcag gcactgaggg caagggatgc ggcgaggacg ccgacgaccg
     3301 caagggtgcg gtgagtgtac atagtgtttt tccaatcagg agaaggtgtg ggtatgaacg
     3361 ggtgccgcgc gcgcgtgcgc ggcccgatca ggttctgctg acggaaagtt gggtgaggcc
     3421 gggcgtgtga cgcggtcgag cgagcgcgcc ggagctgcct cgctcttcga tcacgcggaa
     3481 catccggctt gcgtcgcccg gtcgaggcag gagcaggagt agcagcagca ccagcgccat
     3541 gactccggcg acgcacagaa tcgtcaccag agcgtgcccc gcgtcgcaca taccagcggc
     3601 gcaagtcggg tgagtcgcgg ccgcagtggc cgagtgatgc atccccgaca tgtcatccgc
     3661 cggcgccatc acgtgagtgg tggcagacat ggcgcccatc gacgacgacc cggaggtcat
     3721 cgcatggatg ctcgcagcgc cgagaacgag cgccgaaact acaagggcgg cgatgatcac
     3781 gcagcgaagt gcgcgaggca tcacatccat gccgcccacg ctaggtgggc aacctgtgcg
     3841 tcagctagat tccgacgcgc acgatgtagt agttgctgtc gtagatcggc cgaacgtgga
     3901 taagatcccc gtagtcgccg gagtcgatga agttgccatc gcccatatag ataccggtgt
     3961 ggccgggcat gatgacgatg tcaccgggca gggcatccga cgtcgcgatc ggcgtgccgg
     4021 aggccccgat acccgccgct gagtgcggca gagcgatacc gaactgcgcg tagacgaaca
     4081 tgacgtagcc ggagcagtcg aagcccgacg ggtcggctcc cccgtaacgg tagggaacgc
     4141 cggtgtactg cagcgcgacc gaaacgacct gatcgaggct gaagttcggg tagggcgggt
     4201 tcgccaggaa gtcggccgcg ctgggtccgg tgtacgcgga gtactcgacg gcgagcgccg
     4261 cgcgcgccat ctcggcagcc gatgtggtgg tgaacccatc gcgcgacacc gtggcgacct
     4321 ctgccttggc tgcgacgacc accgtctgcg cgtcgcgctg ggtgatctcc tggagggcct
     4381 gcgatgcctc cacccccgac gtatcgggcg cgggtgagaa agcgtacgcg ggaagggcaa
     4441 ccgtcgcgaa gatccccggc acgatgagtg ccatcaccgc gaggctgaga atgctcgact
     4501 tgcggttcga tgagggacgc ttggcccgga atgccgcggg aacttcggag ccgactgagt
     4561 cgacacttcc gggttctact gcggtggagc tggtcttgct gggtcctact tttgtcaaga
     4621 gatttgacct cctgcgcctc ggcaactcta ggtcgttgcc ccatccgttt cgtatatcga
     4681 gtcacgtacc tggatgagac gggctagcga gacgctcatt cagggtcctt ttgaccaagg
     4741 ttgcggtcga tgaggacttg gcgagtgtac gtgacgattc tgggaaagtc acatcaacac
     4801 gttcgcctca gcgaacattc ggtggaattc tcagcgagcc acgaagatgt gcacagcgac
     4861 ctcgttgggc agctcgagac cctcgccgaa gccctcgacc tgcacgaaga cgtaggagcc
     4921 tgcggccgag atcgtggcca gtgcaccggg catgacgccg gcattctgca actgctgcag
     4981 cagctccggc tcgaactgca ccggttctcc caggcgacgg atgacgccgc tgaccggctc
     5041 attcgtggca gccaccacgt ctacgaggtt gatcacgcca tccatgaagg cgggggcagg
     5101 ggcatccccc agctcggcaa gaccggggat cggattaccg tacggagact cggtcggatg
     5161 cccgagcatc tcgagcaggc gacgctctac ctgttcgctc atgacgtgct cccagcggca
     5221 ggcctcgtcg tgcacgtagg cccagtcgag gccgatcaca tccgcgagaa ggcgctcggc
     5281 caagcggtgc ttgcgcatga cgtggatggc ccggttgcga ccctcggggg tgagctcgag
     5341 gtgtcggtca ccctcgacga ccacgagtcc gtcgcgttcc atgcggccga tcgtctgcga
     5401 caccgtggga ccggagtgcc cgagccgctc cgagatgcgg gcacgcaggg gcacgatgtg
     5461 ctcctcctcg aggtcgagga tggtccgcag gtacatctca gtggtgtcga cgaggtccgt
     5521 catgaaggta agcctaaccg ggccagacaa taggattgtc cccatgccca gcctcgtgat
     5581 tccctcttct ctgctgcccg ccgacggtcg gttcggctgc ggcccgtcga agatccggca
     5641 ggagcaactc gacttcctcg tggccaacgc cgacctgctc ggcacctcgc accggcaggc
     5701 cccgatcaag gatctggtcg gtcgcgtgcg cgcgggtctg ggcgagctgt tccgcatccc
     5761 ggacggctac gaggtcgtgc tcggcaacgg tgggtcgacc gcgttctggg acgccgcggc
     5821 ctactccctc atcgaccagc gcagcgagaa cctcaccttc ggcgagttcg gtgccaagtt
     5881 cgcgcaggcc gcgggcgcgc cgcacctcac ggcgccgcac gtcgtgtcgg cgccggcggg
     5941 gagccgagca tccgtggaga tcgtcgacgg catcgacgtc tacgcctggc cacagaacga
     6001 gacctcgacc ggcgtgatgg cgcccgtcac ccgggtggat gcgccgggcg cactcaccgt
     6061 gatcgacgcg acgagcgccg ccggcggcat cgacttcgac tcatcgcagg ccgacgtcta
     6121 ctacttcgcc ccgcagaaga atttcgcgag cgacggcggg ctctggttcg cgctgttctc
     6181 ccctgccgcg atcgcgcgcg tcgaggcgat cgcggcctcg gaccgctaca tccccgagtt
     6241 cctgtcgctg aagaacgcca tcgacaactc gcgcctcaac cagaccctca acaccccggc
     6301 gatctcgacg ctgctgctgc tgcagaacca ggtcgactgg atcaacggca acggcggcct
     6361 cggctgggcg tcggcgcgaa cggccgagtc atccggtgcc ctgtatgagt gggcggatgc
     6421 ctcgagctac gccaccccgt tcgtcaccga ccccgcgcac cgctcgcagg tcgtggtgac
     6481 gatcgacttc gacgaatcag tggatgccgc ggccgtcgca gcagccctgc gcgccaacgg
     6541 cgtcgtcgac accgacccgt accgcaagct cggacgcaac cagctgcgcg tcgccacgtt
     6601 cgtggccatc gagcccgacg acgtgcgcgc gctcatcggc tcgatcgaat acgtcgtcga
     6661 gcacctctag ccgctgagct ggttgagtag cgccgaaggc gcgtatcgaa accgctccgc
     6721 gctaccctgt acccgtgcga ctctggctga aggactccga gcgccggccc gatccggcgc
     6781 cggtgaagac ggatgaccgt aagccgatgc tgatcggaat cctcgtctgg ctcgtggcgc
     6841 tggccgtgct gctcttcttc atcgccccgc tcaccgccgc ccacaacgag tggtggctgt
     6901 ggacctgcat cgcgggcatc gcgctcggcc tggtgggaat cctgtacacg aactggcggc
     6961 acaagtagcg agttagaagg accgctcgcc ctcgtccgac tcgtcgtcgt catcatcgtc
     7021 atcgtccgac tcatcgtcgt cgtcgagatc ctcatccgga tcgtcgatgt ccacgccgtc
     7081 gaggtcgccg ccgtgcagca cgtcgctgcc cagcacatcg tcgtcgtcca tcacgtggtc
     7141 gtcgaggtcg tcgtcctcgt catccgactc gtcgtcggcg acatcgtcct cgtcgtcggg
     7201 gtcgtcgtcg tcagcgtcgc cgtcttcgac cgcagcctcg cccagagctt cctgcgccgc
     7261 cttgtactcg gcgaggcggt cgctccacgg cagccagtcg ggcgcgagca gcgagccttc
     7321 gcccggcagc aactcggcct cgagcactgt cggctcgccg ccctcgacgt cggcgaccga
     7381 gacggtccac agccagtccg ggtagcccgg ctgggccgat gcgaagcgca ccgtcaccga
     7441 accgtcatcc tcgggggtct ggctgacgaa cgatccgatc gtcgcgaccg gagtgatctc
     7501 cagcagtgcg ctgcgcgcga ggtcttcgta cgactcaggc atccaggtcg tccgcgacct
     7561 tgcgcagcat cgcagcgatc ttcgacgagt gtgacttgtc ggggtagcga ccgcgcttga
     7621 ggccggcacc gatgccatcc atgagcttca cgaggtcctc gatgatgatg gacatgtcgt
     7681 cggccgactt gcggttgagc ttggccatcg acggcggtgc ctcgagcaca cgcacggaga
     7741 gcgcctgggc gcccttcttg ccgtcggcga ttccgaactc gagcttggtg cccgccttga
     7801 ttccggtcgt gccggtgggc agggcggacg cgtgaaggaa gacctcctgg ccgtcatcag
     7861 aactgatgaa gccgaagcct ttctcgtcgt cgtagaactt gaccttgccg gtgggcatcg
     7921 tggtactcct tattgctggt gcatcaaggc tatcggagtc acgcgtatcc ttgggtttgt
     7981 ggccaaccag actccgataa ccgatcaccg cgccgaacgc gtcctggcgt tcatggtcgc
     8041 cagtgcgatc gggctctcga tcatcgcgtt cctcgcggtc atcatcggta ccgcgaccgg
     8101 cgtgacggac ttctccggcg gcgtctggcc ggtggcgatc gtgcttccgg cgatcggcct
     8161 gccgatcgga ctcgtgctca tgatcgccct gatcatcgtc agcggcctgc gtcggggtcg
     8221 tgaagcaagg gatgccagca agtaacgaca gcgccctggt cctcgcgggc cagctgcgga
     8281 gcctgagtga cgcggcgctt gccgagttgc tcacggcccg cgagatcaga ggcacgggca
     8341 tccgtgactt cttcgacctc gctgacgccc tactcgaccc agccgcgatc cagcacgcac
     8401 tgggtcggct ggaccgatcg accctggcta ccctggccgc cctcggcgag ctgggcccgg
     8461 tgaccgaagc ggatgccgcg gcgcacctca ccaccctagg agccgacgca gcgctgctgc
     8521 ccgaacgcct cgccaccgcc gcgcgtctcg ccctgctcac ccagcacctc ggccgctggg
     8581 tggtaccggc ttccgtggcg gaacaactgc agctctggcc gagtctcggc ctgccgagcc
     8641 tgcacgaact cgtgtcgttg tcggcccctg cggcgctcag cccggtgtcg ggtgtcgacg
     8701 tgaagttcac cgatcacgtg gcatccgaga acgcattcca gacgaccacc tcgatcgccg
     8761 agctcatcgc caccctcgac ctggagtcgg cccgcgagct cgcccgcggc ggcatcgctc
     8821 tgcccgacag caagcgcctc gcggcggcca tgggcgtcga tctcgatcgg gtgtccggcc
     8881 tgctcgacat cgcctcccgc gccggcctcg tcgccctcga ctcgggtcgc tggatgcccg
     8941 ccgaggccag ccgcgcgtgg atggtcgact cgtccggcga gcgctgggcg cttctggcga
     9001 ccgcgtggct ggagcgccta cctgtcgaca tccgcacgat tctgcgcgag cgcgcccacg
     9061 ccgtctgggg tgagcggctc gaggagtacg tcgcgtggct gttccccgcg ggcgacgact
     9121 ggatgcgcga ccgcgtgcgc gtctacacgc gcgacgccga gctgctgggc atcaccgccg
     9181 acaacgtgcc gagcaccccg ggagcggcac tgctggcgag cggggcggat gccgcggcca
     9241 ccgccatggc ggcgctcttc ccgcccgagg tggcgcaggt ctacctgcag cacgatctgt
     9301 cgatcgtctc cccgggaccg ctcgcgccac ccatcgatgc gcgcctgcgc acgatggccg
     9361 acgtcgaggg ccgcgcgctc gccagcacct accgggtctc gaccgccagc ctgtcgcgag
     9421 cgatggcggc gggcgagacg gagagctcga tgagggagtt cctcggcggg atcgcgctga
     9481 cgggcatccc gcagcccctc gattacctgc tcaccgaggc ggcatcgcgc tacggtctgg
     9541 tacgcgtggg cgcgaccccc gagggccgca cctatgtgtc gagcaccgac ggcaccctgc
     9601 tgcgcacgct cgtggtcgat cacagcatcg gctcgctcgg gctggtgcgc tccggcgacc
     9661 ggctggagag ccgctacgac cgcgacttgg tgttctgggc gctgagcgag gcccgctacc
     9721 cggtcgcagc agagaacgcg gccggcgaga tcgtcacgct cgctcgtcgg caggcctcgc
     9781 gcatcaccgc cgcagccgcc accgacacca ccggcgcgct catcgagcga ctgcgggtgg
     9841 gcagctcggc cgatccggat gtcaccggca aggcctggct cgcacgccag ctcgacgtgg
     9901 ccatcaaggg caaggtcggc ctcacggtca ccgtgcggat gccggacggt tccgacgtcg
     9961 actaccagct ggagccggcg agtgtcggcg gcgggcggtt gcgcgcccgc gatcgccgca
    10021 gcgacatcga acgcaccctt ccgctgacca gcatcgtggc ggtagcgccc gcggagtagc
    10081 ggctggttga gtaggccgca ggccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnncgaaac ctcgccgcga tactggtttc gatacgcggc ctcggccgct actcaaccgg
    10261 cgaaccaccg taggctagac ggatgcccac caacggtccg ctcatcgtgc agagcgaccg
    10321 cacggtgctg ctcgaggtcg cccaccccga cgccgaggat gcccggcacg acctcgccgt
    10381 cttcgccgag ttggagcgtg cccccgagca catccacacc taccgcatca cgcgcctcgg
    10441 cctgtggaac gcgcgcgccg cggggcacac cgcggccgac atgctcgcga cgctcgagaa
    10501 gtactccaag ttcgcgatcc cgcagtcggt ggtcgtcgac atgaccgaga ccgtcggccg
    10561 ttacgggcgc ctcgtgatcg agcgagcgga tgacggtgcc ctcacgctca ccgcaaccga
    10621 cgccgccgtg ctcagcgaga tcacgcgcgc gcgcaaggtc gctgccctgc tgttcgagaa
    10681 gcgcggggat cacacgtacg tggtgcaggc ctgggctcgc ggccagctca agcaggagct
    10741 gctcaagctc ggctggcccg ccgaggatct cgccggctac acccccggca ccccgcacga
    10801 gatcgcactc gacgagtcgg cgtggcacct tcgaccgtac cagcaccagg cgatcgacaa
    10861 cttcgcggcc cacgggtcgg gcgtcgtcgt gctgccctgc ggcgcgggca agaccctcgt
    10921 cgggctcggc gcgatggccg ccggcaagac gaccacgctc atcctcgtga ccaacaccgt
    10981 ctcggcccgg cagtggaagg ccgagctgct caagcgcacg acgctgaccg aggaggagat
    11041 cggcgagtac tccggtcagg tcaagcaggt gcggccggtg acgatcgcga cgtaccagat
    11101 cctcaccgcc aagcggcagg gcgagtacgc ccacctcgcg ctgctggacg cgctcgactg
    11161 ggggctcgtg atctacgacg aggtgcacct gctgcccgcg cccgtgttca agttgaccgc
    11221 cgaactgcag gcgcggcggc gcctgggcct caccgccacg ctcgtgcgcg aggacggccg
    11281 cgagggcgac gtgttctcgc tcatcggtcc caagcgcttc gatgcgccat ggaaggagat
    11341 cgaagcccag ggcttcatct ccccggcgag ttgtttcgag gtgcgcatcg acctgcccga
    11401 gaacgagcgg ctcgagtacg cggcatccgc cgacgacgaa cgctaccggc tcgcggcgac
    11461 agcaccggcg aagctcgggg tggtgcgcga actggtcgcg aagcacgcgg gcgagcgcat
    11521 tctcgtgatc gggcagtacc tcgaccagat cgatgagcta gcggatgcgc tcggcgcccc
    11581 caagctgacc ggcgcgaccc ccatcgacga gcgggagcgg ctgtaccagg agttccgcga
    11641 cggcgtcacg aaggtgctcg tcgtctcgaa ggtcgccaac ttctcggtcg acctgccgga
    11701 ggcgactgtc gcgatccagg tctcgggatc gttcggctcc cggcaggagg aggcgcagcg
    11761 tctcggccgg ctgctgcgac cgaaggagag cggactgccg gcgaatttct acacgctggt
    11821 cgcccgcgac accgtcgacc aggacttcgc gcagaaccgc cagcggttcc tcgcggagca
    11881 gggctacagc tacacgatcc tggacgccag ttcgctggtt gagtaggccc gcagggccgt
    11941 atcgaaaccg agccgcctag cgctgggctg gttgagtagg cccacggggc cgtagcgaaa
    12001 cccgcccgcc cccgcctgtc gggcgaccga tgcacaatgg gagcatgacc gtcacaccct
    12061 tcgtgatcga tgttccgcag tccgacctcg atgacctgcg cgcgcgactg cgggcgacgc
    12121 ggtggccgga tgccgcggtc tccgactggt cgcagggcat ccctcctgcg gctctccgcc
    12181 gcgtgctcga ctactgggct gaggagtacg actggcgcgc cgtggagcgt caactcaaca
    12241 ccctcaccca cgtcaccgtc gacggcatcc atgcggtgag ggctggcacg cccggtgcca
    12301 cgcccctgct gctcgtgcac ggctggcccg atggcttcgt gcgattcctg cgcgcccttc
    12361 cgcttctcgc cgaccgcttc gagttggtga tcccgagcat ccccggctac ggcttcagcg
    12421 cgcagcccgc cgagccgctc ggaccgatcg gtgtcgccga tcgtttcgtg gatctcatgt
    12481 cgcagttggg ccacgagcgg ttcgccgtgc acggcggcga catcggaacg cagatcgccg
    12541 atcagctcgg cctgcgtcat ccggatcgca tcacggcgct gcacctcggt gacgttccgc
    12601 tgcgccgcct gcgcggcatc gaccccgccg agcagacccc cgaggaacag cagtggtcga
    12661 aggatggcgc ggcctgggag ctcgccgagg gcgcctactc gcacctgcag agaaccaaac
    12721 cgcagaccct cgccgccgcc ctcaacgact cccccgccgg gctcgccgcg tggatcctcg
    12781 agaagttcga ggcctggagc gacaagggtc tcgacgcgtt caccctcgac gacctcgcca
    12841 ccaacctcac gatctactgg gtcacgcaga ctgcgggttc ggccgcgagg tactactacg
    12901 acaacactgt gacgcccacc ggtcgtcagg tcgtaccgac gggtttcgcg gttttccccg
    12961 cttacatctg cccgccgccg cccggctccg cgagccggtg gtacccggtc gagcgctgga
    13021 cccagatgcc gcgcggaggc cacttcggtc cgtgggagga gccggagctg tgggccgcgg
    13081 aggtacgggc cttcttcgat cagctggagc tgtagcgcgg ggcgggtttc gctccccgcg
    13141 cgcacggccg tcgcgccgct ccgcgtcgcg ccggaaccgg cgacgtgggc ccagcgctgc
    13201 gcgctactca accagcgtgc gggcgtagcg cagcagcagt tcttcgccgc cgcgcagcac
    13261 atgcacgagc cgcatcgacc gcgccgggat atccccgccg acgattcgag ggccatcccc
    13321 gccctcgagg gtcggcgcga ccgtgaggca caactcgtcg atcaggtcgg cggccagcat
    13381 cgccgcgaag agcgacggcc cgccctcgca cagcacctgc gcgtggccca tcgcgcgcag
    13441 tgccgccaga gccgcagcga ggtcgacgtg ctcgtcgccc accgccacga cgtgagcgac
    13501 agcggcgaag gccgtggtgt ctgtccccgc ggtggtgatg acgatcggtc gcacgggcgc
    13561 ctcggtgaag atgggcgagg tcgggtcgag gtcgaggtga cccgacacga tcgcaaagac
    13621 cggatgccgc ggcatcccgt gccgttcacg ccagagttgc gacgcctcgt caaccagcat
    13681 cggcccgtag ccctcgtcgc gcaccgttcc cgcgccgacg agcacgacgt cggtgacccg
    13741 acgcagcaac tcgaagtggc gcttgtcggc ggcaccggag agcccgcccg aacgcccgtc
    13801 gtgggtcgcc gcgccgtcga tgctggcgac gaagttcgct cgcacggtgg gcgccgcgac
    13861 cgcgagcgag tcgatcagcg cgtcgtcact gaggccccgg ggcgcacccg gccagagcat
    13921 gtcgatggcc gcgctcatac gttcagggtg ggatgcgtgt tgtggagcag gtacgccggc
    13981 tcgcggaatc cgagaatcgc ctccgtcatc cgcactgcgg caactgacgc cgcgacgttg
    14041 tgcatgcgca cgatgcgggc gcccttcatg atgcagacga ccatcgccgc cagcgacccc
    14101 tcgagccgtt cggtgcgctc gcgatcgagc acctcgccga tgaagtcctt gttcgagacg
    14161 gccacgagcg tcgggtagcc gagcgcgacg acctcctcca gccgacgagt cagttcgagc
    14221 gagtgcagtg tgttcttgtc caggtcgtgt ccgggatcga tgatgatccg ctcggccgga
    14281 acccccgcct gcagcgcgcg atccacccgc gcgcgcagga agtcgacgac ctcccgcacg
    14341 acatcgtcga agtgcgcagc gggcttctcc acccggggcg gcccgacgct gtgggtgatc
    14401 acgacgtgcg ccgagctctc ggcgatcact gccgccatgt cagggtcgcc gagcccgctc
    14461 gtgtcgttga tcacggcagc gccagcggcg atcgcggccc gtgcgacctc ggcgttgttg
    14521 gtgtcgaccg agatgaccac atcggatgcc gcggcgatcc cctcaacgac cggaacgacc
    14581 cgcgcgatct cctccgccac cgtcacggcc ggcccacgac cgaagggcac cccgcccacg
    14641 tcgacccagt cggcaccctc ggacgcggcg gtcagcgccg cgccgatcgc gcggtcgagc
    14701 tcgaacgtcg ccccgcgatc gtagaaggaa tcgggggtgc ggttgatgac cgccatgacg
    14761 gcgatctgcc ggtcgaagtc gaagtcgcgc tggccgatgc gaggcatacg acaaccgtac
    14821 gtcgtcgcac ggccgcggcg gagcctagac ggtgcggcga cgccgggcgg cgagcagcag
    14881 cagcgtgccg agcgccagtg caatgctgcc tgcgatcacg ggcgccgtgg catccacgcc
    14941 ggttgcggcg agagcgactt cgaacgggaa gtaagcgaag acgatcttgc gttcgtcgaa
    15001 caccgcgtac acggcgaccg agtgcgtgcc ggcggccagg ttcgccggga gggcgacgtc
    15061 agcgctgaac gcgaagtccg gggccggagt agtgaatggt ccagcgacgt cgaccccgtc
    15121 cacggtcacg tagtaggccg aggccaccgg cggcagcagg ccggccgccg tgatcgtgac
    15181 ggtgcgcccg ggagcgggtg ccggcgatgc ctcgatcgtg cccgacaccg ggatgacgcc
    15241 gccgcccgta ccgccgccgt tgccggagcc gtcggggaag tccacgccgc caccgcacga
    15301 cgcagcagga gacaacgaga cggcgcccga ccagatcggg tagtaccccg tggcaccggc
    15361 cgccaggtcg cccgtgatcg cggtgatcgt gggcccaccg gtgtgcgaga ccgtaccggt
    15421 gaaggaggag gcgaacgtga acgcggcgtt cgagttgttc gtcacgacgc accgcgcggc
    15481 ggtggtgccg aagaatggca cgccggcgaa gctcccgtcg atccaggcac ccgaggcgtc
    15541 ctgccactcg aggaggacgg tgggggcgac gttggtcacc gtgaagatcg tgcccgtgta
    15601 gtcgaccgtc accgccgact gggtgaatcg cgcggtaacg gaatgcgcac ctgcggtcag
    15661 tgccgacacc ccatccgtgc acgaggtgaa gcctgtggca acagtgcagg aggcgatcag
    15721 ggtcgcaccc tcatacagct cgaaatcgac atccgcggcc agcgcggcac cgagtgtcac
    15781 ctgcacccca ccaccggacg acagcgcgta tgccgtgcca ccgagaagtg agtcgttgga
    15841 tggctgcaac gtcacatcca ccgtcgtgcc gaacagcggc tgcgatgccg cctgagcggg
    15901 cgcggccgcg agcagggctc ccacgagagc gagcgggagg gcgaggatca cgagggcagg
    15961 tcggcgcacg atccgaatct agtggaccgg cggcacgcgg ggcgacaccg gttcatgttc
    16021 gcgtcgccga ggcatccaca cgactctcag acaacttcca gagaactcgc agatactgta
    16081 cgcatggctg atggacccaa gattctgatc gtcgacgacg agcccaacat tcgcgacctg
    16141 ctcaccacga gcctgcgctt cgccggcttc gccgtgcgtg ccgtcggcaa cggtgcccag
    16201 gcgatctccg ccgtactcga ggaggagccc gacctcatca tcctcgacgt catgctgccc
    16261 gacatgaacg gattcggcgt gaccaagcgc ctgcgggcat ccggctacac ggcccccatt
    16321 ctcttcctca ccgcgaagga tgacaccgag gacaagatca ccggcctcac cgtcggcggc
    16381 gacgactacg tcaccaagcc gttcagcctc gacgagatcg tcgcgcgcat caaggccatc
    16441 ctgcgccgca cgatgcacga cgacgaggag gcgatcatcc gcgccggcga cctcaccatg
    16501 gatcaggaca cccatgaggt gaccatcggc acagagcagg tggagctcag ccccaccgag
    16561 ttcaagctgc tgcgctacct catgctcaac ccgaaccgcg tgctgtcgaa ggcgcagatc
    16621 ctcgaccacg tctgggagta cgacttcaac ggcgacgccg gcatcgtgga gagctacatc
    16681 tcctacctgc gtcgcaagct cgaccagtac tcggaggagc cggtcatcca gaccaagcgg
    16741 ggcttcggat acatgctcaa ggcctcgaag gtctagcgca gcttctcgac ggtaaactca
    16801 gggctcaatg cacgagatcg tcgagtggtg gaaccgggtt tccctccgta ccaaaatcac
    16861 cggcgtgacg gttctgctgc tcactgcggg cctggtgatc gcgggcgtcg gcacgatggc
    16921 ggttctgcgc ggctatctgc aagacgaagt cgacaccaag atcaccgccg ccggcaacaa
    16981 gctggctggc aacatcctct tcgctggcac cgccgacaac ccgcagtgca gtgtctcgac
    17041 cctgcccaat gactattacc tgggcgtgct cgactacaac ggcgtgctgc tctgcaacaa
    17101 ccgcgaagag gatgccccgc gacccgttct cgacacgctg acgctgggcg ccgtcgcggg
    17161 cagtgaaggc gcattcacgc tgccgagcac cgcgctgggc ggatcgtggc gagtgatcgc
    17221 ctatcccgcg accaaccgtc tcggcaccga ctactacacg gtcgtgatcg gcctcgacct
    17281 gaaggacacc aacgggatcg tcacgcgcta cgcggcgatc ttcctgttct tcggcctcac
    17341 cgtggtcatc ctcggcggcg cgctcacccg tctcctcgtc accacgacat tcgcaccgct
    17401 gcgcgaagtt gaggccacag cagcccgatt cgccggcgga gacttcagcc agcgactcag
    17461 cggagcgacc cccaacaccg aagtcgggcg cctcaatcgc tcgctcaact cgatgctctc
    17521 ccgaatcgac cgggccttcg acgagcgcgc caagacgatc gaccagatgc gtcgattcgt
    17581 gggcgacgcg agccacgagc tgcgcacgcc actggtaacc gtgcgcggat acgccgagct
    17641 ctaccgcatg ggcgccctga ccaagcagga ggatgtcgcc caggcgatgg agcgcatcga
    17701 gaaggaagcg atccgcatgg gcgaactcgt cgaggacctc ctcgagctcg cccgcatcga
    17761 cgaggcgaag ccgttgcagc tcaccgaggt cgatctggtg ccgctcgccc aggacgccgc
    17821 cctcgacacg atggccagcg cccccaaccg cgtcgtcacc gtcgtcggca cggatgccac
    17881 gaccgaggag ccgcccgctg agaccgccgt gctccccgtg gtggcgccga cccccgccca
    17941 gcagcgcggc gggctcgcta ccggacccat cgcgttcgcc ggagccacgc tcgcccggtt
    18001 gaccgcgcgc cgcacccgcg ccaaccgctc cggcggtgac gaggtgacga ttgccgagcc
    18061 cccgcccgcc gaactgcggg ccgtcgtgct ggccgaggag aacaagatcc ggcaggtgct
    18121 gtcgaacctg atcggcaacg cgctgcggtt cacgcccgag tccagcccca tcgagatcgg
    18181 ggtgggggtc gatgccgcca gacagcgcgc gatcctgtcc gtgatcgacc acggtgaggg
    18241 catcccgccg cagattcgcg gcaagatctt ccagcggttc tggcgtgccg acacctcacg
    18301 cacccgcgac accgggggaa gcggcctcgg cctcgcaatc gttgcggcga tcatccaggc
    18361 ccaccagggt gacgtcgaag tggtggagac ccccggtggc ggtgcgacct tccgggtctc
    18421 gcttcccctc ctcccctcga ccccggatgc cgccgcctcc ccatcacgcg cctgaccccg
    18481 tcaccggctc acgccgccgg gctaccgtca ccccatgact cgttatcagg tagacagtga
    18541 agcagttctt caggccaccg gtgccgtgcg cacctcgatc ggccgcatcc aggcggaggt
    18601 ggccggtctt cacggccaac tcagcgcgct ggaggggtcg tggaccggcg cggccgccac
    18661 ggcgttctcc ggcgtcgtga ccgactggaa ggccacccag cagcaggtcg agcagaacct
    18721 tgctgcgatc aatcacgccc tcacccaggc cggtcagcag tacgcggaca tcgagtcggc
    18781 caacacccgg ctgttcgcgc gctagtcccc ccgagaacga cgaatgggcg gctccccgga
    18841 gggaaccgcc cattcgtgtg acttgggtct cgatacaacc ggccggaagg gccggtcaga
    18901 tttcgatacg gccttgcggc ctactcaatc cgcgctcgac ccgattgatc acatctgacg
    18961 ctaagcgtca gccgcgatca aaagtccatg ccgcccgacg ggtcacccat cggaggagcc
    19021 gacttctcgg gcttgtcagc aacgacggcc tcggtggtga ggaagaggcc ggcgatcgac
    19081 gctgcgttga gcagcgcgga ccgggtcacc ttcaccgggt cgttgatgcc ggcagccagc
    19141 atgtcgacgt actcaccggt cgcggcgttg aggccctgac cggcgggcag gtggcgcacc
    19201 ttgtcggcga cgacaccggg ctcgagaccg gcgttcagcg cgatctgctt gagcggggcg
    19261 tcgatggcca cgcgcacgat gtttgcaccg gttgcctcgt cacccagcag ggccttgagg
    19321 gccttgccat cgaacgcgat cttgccggcc tggatgagcg cgacgccacc accggcgacg
    19381 atgccctcct cgacggcggc cttcgcgttg cgaacggcgt cctcgatgcg gtgcttgcgc
    19441 tccttgagct cgacctcggt cgcggctccg gctttgatga cggcgacgcc accggcgagc
    19501 ttcgcgaggc gctcctggag cttctcgcgg tcgtagtcgc tgtcggtgtt ctcgatctcg
    19561 gcacggatct gcttgacccg tccggcgatc gcgtcctcgt cgcccgagcc ctcgatgatc
    19621 gtggtctcgt ccttggtgat gatgaccttg cgtgccttgc ccagcaggtc gagggtggcg
    19681 ttctcgagct tgagtccgac ctcctcggag atgacctggc caccggtgag gatggcgatg
    19741 tcctgcagct gagccttgcg acggtcaccg aagccgggag ccttgacggc gaccgacttg
    19801 aagatgccgc ggatcttgtt cacgaccagg gtcgcgagcg cctcgccgtc gacgtcctcg
    19861 gcgatgatga gcagctgctt gccgctctgg atcaccttgt cgacgatggg cagcaggtcc
    19921 ttgatgttcg agatcttgga gttggcgatg aggatgtagg ggtcctcgaa cacggcctcc
    19981 tggcgctcct gatccgtcac gaagtaggcg gagaggaagc ccttgtcgaa gcgcatgccc
    20041 tcggtgagct cgagctcggt gccgaaggtg
//
LOCUS       DAOSOU010000010        19956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2704_length_19956_cov_7.503894, whole genome shotgun sequence.
ACCESSION   DAOSOU010000010 DAOSOU010000000
VERSION     DAOSOU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 19956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19956
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2704_length_19956_cov_7.503894"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLW15_01120"
     CDS             <1..823
                     /locus_tag="PLW15_01120"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOT33031.1"
                     /translation="VIRRYSTSFGLASRLLGSGVRQHVENIYALVRVADEIVDGGAEE
                     AGVDTIGAGRALNELERETENAMDTGFSSNLVVHAFALTARETSFGAELTAPFFESMR
                     MDLSAKQHDQASFERYVYGSAEVVGLMCLRAFVLGEKVDDLPRLETGARALGAAFQKV
                     NFLRDLAADFETLGRSYFPNVNVETFTEEEKVRLLDDIDADLAVSAAVLPDLPASSRR
                     AVALAQSLFAELSRRLRKTPADQLVRSRVRVPNPVKVRLALAASAGSLPRSKGRS"
     gene            820..2487
                     /gene="crtI"
                     /locus_tag="PLW15_01125"
     CDS             820..2487
                     /gene="crtI"
                     /locus_tag="PLW15_01125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOT33032.1"
                     /translation="MSRVVVIGGGIAGLASAALLAREGHEVTLLEGRPEVGGRAGSWE
                     QDGFRFDTGPSWYLMPEVFDHFYKLLGTSADEQLKLVTLDPGYRVYFEGVAEPLDVSA
                     ELEDNLEMFEAIEPGAGDRLEKYLDSARETYELAKLRFLYTTFADIRTLLRRDVLSKT
                     GKFLRLLLEPLSSLVGRKFHDQRLRQVLGYPAVFLGSSPYLTPSMYHLMSHLDLADGV
                     LYPIGGFTRVIESIADLARAEGATIITRAEVTRIVVENGKATGVEYLDEHREIQFADA
                     DIVVSAADLHHSETELLKPEEQTYPQEYWDTKTPGPSALLLYLGVEGELPQLEHHTLL
                     FTREWERGFDAIFGTPSSVPEPASLYICKPGGVDPDVAPEGYENLFVLVPIPADPTIG
                     RGDVDGDGDTRIEVLADRVIAQIADWTGIPDLASRITVRRTVGPGNFADELHAWRGTA
                     LGPAHTLKQSAFFRAGNVSKKVDGLYYVGGSTIPGIGLPMCLISAEILIKRLRGDTST
                     EPMAEPLVPSAVDAPVVELVETPAQISTGSITEAGSITEAGSITEAR"
     gene            2484..2867
                     /locus_tag="PLW15_01130"
     CDS             2484..2867
                     /locus_tag="PLW15_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HOT33033.1"
                     /translation="MSFLYLAALAVALTGMVMLDRRFRLFFWAAPWRAAVVLVVGLAF
                     FLAWDLAGVGLGVFFRGETSFMTGLQVAPEVPLEEVFFLTLLCYLTMNAYAAASLLLQ
                     ARSLRTHAGAEDGRDVRNDSAGGAA"
     gene            2864..3217
                     /locus_tag="PLW15_01135"
     CDS             2864..3217
                     /locus_tag="PLW15_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HOT33034.1"
                     /translation="MTYWALNVVFLVPVAVLVIAGLIVRRTPRWPAVALTAAVVLVMT
                     AIFDNVMISVGLVAYNPAAISGAFVGVAPLEDFAYAVAAVVGLPALWALLGGRRSSSP
                     KGVSRPAPEEEPTDA"
     gene            3210..4070
                     /locus_tag="PLW15_01140"
     CDS             3210..4070
                     /locus_tag="PLW15_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005273305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOT33035.1"
                     /translation="MLKALFVSSRPLSWVNTAFPFAAAYLLTTGQVDVTLIVGTIFFL
                     IPYNLAMYGINDVFDYESDLRNPRKGGVEGALLDTRMHRPTLIASVVTTVPFVVYLVI
                     VGSPLSWLVLAISLFAVVAYSAKGLRFKEKPFLDSITSSTHFVSPAVYGLVLAGAVFT
                     TQLWAVLGAFFLWGMASHAFGAVQDVIADREGGIASIATVIGARWTVRFAVIAYLLGG
                     VLLLFSAWPGPLAAILVLPYAAVCAPFWNVTDAAADTANRGWRRFLLLNFITGFFVTM
                     LMIWWAFTAR"
     gene            complement(4057..4896)
                     /locus_tag="PLW15_01145"
     CDS             complement(4057..4896)
                     /locus_tag="PLW15_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33036.1"
                     /translation="MPAARVALATLSRVAPALGAEVGWRLWRHVGTPQPVHERDSLVH
                     KWAYREVVDAGGREIMTYRWGSGERVILLVHGWRSRASRFSALVAALQAADVTVIAFD
                     APANGDSPGRLVTILDYAAAIEALSLRHGPIDTIVAHSFGVLATFVAVREGVTVKRIV
                     GISGMANADQLVDKFCALAGLGARAVRGMRRRIERRTFPEVVDPWSRFVAELDPSATH
                     VPVLLVHDTDDATVDPGQALLIANAHTGPVHSVITSGLGHLRILSDPAVVEEVRQFVV
                     SGR"
     gene            4971..5579
                     /locus_tag="PLW15_01150"
     CDS             4971..5579
                     /locus_tag="PLW15_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT33037.1"
                     /translation="MTTAADGRRARGDESRRQVMERAVNLASVGGLDGLTIGALAIET
                     DRSKGGVVALFGTKEQLQLATVEAAREIFIERVIAPALTVPGGLDRLRALLDNWLDYS
                     QGRVFAGGCFFAAAAMEVGAQPGPVRDAVADAMADWYETVRRIAQRAIDAGELAPGTD
                     AAQLAFEVTAILEGANSASRLTDSVEPYARARVALAGLVQPA"
     gene            complement(5567..5998)
                     /locus_tag="PLW15_01155"
     CDS             complement(5567..5998)
                     /locus_tag="PLW15_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT33038.1"
                     /translation="MDTPRPIGFWLKLVDRLIDEQFASTLEEHGVTRRQWQLLNVLTQ
                     GPATVEQLDAAVAPFLSGATDDDPAETSADHLAELLDSAWLEDTAQGYALTDRGRGAF
                     ERLELVVAAQRTQVAEGITADQYGQAVAVLERMARNLGYAG"
     gene            complement(6008..6388)
                     /locus_tag="PLW15_01160"
     CDS             complement(6008..6388)
                     /locus_tag="PLW15_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33039.1"
                     /translation="MNTPNETTAPRPSHWLRAIEFRKHQLGREYRDTLNDKAREGISD
                     EDYATTMATLEKMALNLGWSEDDDFGPRGRGFGPGFGRGPKRGFGRGFGPGFGPGHGH
                     GHHDHPHHRPENAQDSSPAAEPEA"
     assembly_gap    6552..6651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6652..7026
                     /locus_tag="PLW15_01165"
     CDS             <6652..7026
                     /locus_tag="PLW15_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOT33040.1"
                     /translation="GHHGPPGLAGAARFRMLEVLESAAPETRMGISEVAAAIGVDQPR
                     ASRLVNDAAERGLVLRSADPRDARRSVIALTDAGRALLDSARAFRRSAVTEALADFTD
                     DETQALAALLTRLAAAWPQAPR"
     gene            complement(7037..7846)
                     /locus_tag="PLW15_01170"
     CDS             complement(7037..7846)
                     /locus_tag="PLW15_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HOT33041.1"
                     /translation="MAASTRQAEGMTQLDKARLLDSLHVPGDPLIVTNVWDAVTARIV
                     AAAPGVRAIATASHAVSYAHGVDDGEALTLDEALDAARRVVAAVELPVSVDFERGYAT
                     DAAAVQHHVEQLIATGTVGLNLEDSSGPDALFPHELAVERVAAVRRAGEATGIPIVLN
                     ARVDALVRGGTRDDAIARANAYLAAGADVIFMLGVRDEESISWAIEHIDGRVSVIANP
                     SAPPLTTLAELGVSRVSFGPGTLGLTLAHLQAAATQLTALGEYPAELGFSY"
     gene            complement(7872..9299)
                     /locus_tag="PLW15_01175"
     CDS             complement(7872..9299)
                     /locus_tag="PLW15_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33042.1"
                     /translation="MTDELPDAVKPTDSKIPVAADTLAVGAVGRVADDDKVVPRKQVI
                     SWAMWDWATQPFNSVILTFVFASLYLVSDNFLPADIAALPDGDAVKEQALAGLTAGYG
                     LATTLAGVLIFLLAPVLGQRADASGMQKRLLLLFTGILALIQFGLFFVFAQPAYFWLG
                     AGLLALGAVVSEIAGVNYNAMLVQVSTRKTIGKVSGLGWGLGYIGGIVALVIVVILTF
                     ADWFGLDTSNGLAYRLIAVGAGVWTILFSLPLFFNVPEAPAARPDIAKIGFFQSYVVL
                     VKDIGKLFKESASTGWFLIASAIYRDGLAGVFAFGGILAAVAFGFTANEVMIFGIAAN
                     IVAGISTIIAGRIDDMVGPKAVILFALFGLVAMAVVIFALHDTGTAVFWAGGLILSAF
                     VGPAQAASRSLLARVTPEGRQGEVFGLYATTGRVASFISPALWTLFIVAFGATIYGVL
                     GIALVLLVGAVALLFVKIPKHVRVR"
     gene            complement(9309..9899)
                     /locus_tag="PLW15_01180"
     CDS             complement(9309..9899)
                     /locus_tag="PLW15_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HOT33043.1"
                     /translation="MTSTSENPVWWQRLGSLPFAVAVLVACNALYIFLVALGNITDYG
                     TNFEFVKHVLSMDTTNFGAADGTGLDPDVMWRAITDPVVWNVAYIGLIIWESLAAIVL
                     IGACVLWLRALITRGTFDSARIWSSIGLLMIIVLFFGGFITVGGEWFQMWRSTAWNGL
                     DPAFRNSVLAAITLVLIHTTAQGWVRKVHVENTSSH"
     gene            10062..11921
                     /gene="dnaK"
                     /locus_tag="PLW15_01185"
     CDS             10062..11921
                     /gene="dnaK"
                     /locus_tag="PLW15_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT33044.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGEPTVIANAEGFRTTPSVVAFTK
                     DGETLVGETAKRQAVTNVDRTIASVKRHMGTDWKQDVDSKKYTAQEISARILAKLKRD
                     AEQYLGEPVTDAVITVPAYFNDAERQATKEAGEIAGLNVLRIINEPTAAALAYGLDKG
                     KEDELILVFDLGGGTFDVSLLEVGKDDDFSTIQVKATAGDNRLGGDDWDARVVDYLIK
                     QFKATTGVDVSGDKIAKQRLKEAAEQAKKELSSQTSTTIQLPYLSLTENGPANLDETL
                     TRAKFEELTADLLERTKKPFNDVIKEAGVKLSDVAHVVLVGGSTRMPAVVDLVKKLTG
                     GQEPNKGVNPDEVVAVGAALQAGVLKGERKDVLLIDVTPLSLGIETKGGIMTKLIERN
                     TAIPTKRSETFTTADDNQPSVAIQVFQGEREFTRDNKNLGTFELTGIAPAPRGIPQVE
                     VTFDIDANGIVHVSAKDKGTGKEQSMTITGGSSLSKEDIERMVREGEEHAAEDKARRE
                     AAETRNTAEQLAYSTEKLSKDNEDKLPADVKTDVQADVDALKSALAGDDEAAVKSAFD
                     TLTASQQKLGEAIYASGQAAPADGETAEPATEGSDEDIVDAEVVDDEPEDGKK"
     gene            11925..12557
                     /locus_tag="PLW15_01190"
     CDS             11925..12557
                     /locus_tag="PLW15_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT33045.1"
                     /translation="MSDNNDERHENEEPVIRDKRRIDPETGDVREPDAAAVGDDTPAA
                     EAVDADGADAAEELSDADQALLDQAAQDLVAEMRSDMLRAQAELVNFRTRVERDRAAN
                     REAVIAEVIRSLLPALDDLARAEAHGDLIEGAPLTIIAQKLRGAFEKYGLRQIGEKGE
                     AFDPAFHEAVVQLPSPDVTVNTIADVIEPGYVLGDRLVRPAKVAVSVPAE"
     gene            12569..13519
                     /locus_tag="PLW15_01195"
     CDS             12569..13519
                     /locus_tag="PLW15_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOT33046.1"
                     /translation="MASQDWFDKDFYAVLGVSKDVSEADLKKTYRKLARQYHPDSNPG
                     DAKAEAKFKEISEAHSVLSDPEMRREYDQIRAMGSGARFAPSSGANGGFEDVFGGMFG
                     NRGTYQGTSFEDLLGGMFGGSNQGFRTGPTAGRDVTASTTLDFVTAIKGETIKLQPAG
                     GAPINVKIPAGVSDGQKIKLKGKGEPSHDGGAPGDLVLTVTVRKHPVFERDGLNLRVE
                     VPVTFAEAALGATIEVPTLGGEPVKLRVAAGTPGGRILRVKGRGVTTAKGTGDLLAEV
                     QVAVPGRVPDDAAAHLRAFEAAMPQENPRAELLDKARSDH"
     gene            13519..13947
                     /locus_tag="PLW15_01200"
     CDS             13519..13947
                     /locus_tag="PLW15_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT33047.1"
                     /translation="MSAPLDEFSPVFAIAVAAELAGMHPQTLRQYDRLGLVSPTRTAG
                     QSRRYSMRDVAQLREIASLSAQGLNLEGIKRILELENQVAALAGRVRELEHALADELL
                     ARPGRRVFAAGGSGEVVSIKAGSRVAKSNQVVVWRPLRRG"
     gene            13953..15083
                     /locus_tag="PLW15_01205"
     CDS             13953..15083
                     /locus_tag="PLW15_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT33048.1"
                     /translation="MAGEQLDTIDDRVLSGLRRWPDVEAPNLHAVDASDRLILDEAAA
                     AIAESGPGEVVVIGDAYGALTIGAAAIGATGIRVFQDSIVGERALAANAAGAVDYVTL
                     PVGADLLRGARVVLMQLPRSLAELDELAAWIASAADPAVRVYAGGRIKHMTTAMNEVL
                     GRHFGRVDVTHARQKSRVLIASQPLPSEPPMIAREFHTDLGIWVCATGATFAGTRIDI
                     GTRALLAVLPTVAPDATTAADLGCGSGVVAAVLAQARPELAVLASDVSAGAVASARAT
                     AEANALTNVTVTRDDALGGQPDASLDLVVLNPPFHLGATVHTGAASKLFAAAARVLKQ
                     GGELVTVYNSHLGYRAELTRVVGPTDELSRTSKFTVTRSRKN"
     gene            15083..17236
                     /locus_tag="PLW15_01210"
     CDS             15083..17236
                     /locus_tag="PLW15_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT33049.1"
                     /translation="MANMQGAPGTDEEQKTALQQYGVNLTDIARSGKLDPVIGRDAEI
                     RRVSQVLTRRTKNNPVLIGEPGVGKTAVVEGLAQRIVAGDVADSLKDKQLVSLDLAAL
                     VAGAKYRGEFEERLKAVLKEINDSDGQIITFIDELHTLMGAGGGEGSVAAANMLKPML
                     ARGELRLIGATTLDEYRQYIEKDAALERRFQQVFVGEPSVEDTVAILRGLKERYEAHH
                     KVTIADSALVAAATLSNRYITGRKLPDKAIDLIDEAASRLKMEIDSSPTEIDQLKRAV
                     DRLRIEELALKKEKDDASKARLEKLREDLGERQKELDALEARWKAEKASLTGVGDLKT
                     QLNDARIDLDRAMREGDYQKASKLNYETIPKIEAALVTAESSEPVGPRMVNDQVTEED
                     IASVVAAWTGIPVDRLTQGETEKLLHLEAELGKRLIGQKDAVAAVSEAVRRTRAGISD
                     PDRPTGSFLFLGPTGVGKTELAKALAEYLFDDEKALVRIDMSEYGEKFSVSRLVGAPP
                     GYIGYEQGGQLTEAVRRRPYSVILLDEVEKAHPEVFDILLQVLDDGRLTDGQGRTVDF
                     RNVILILTSNLGSQFITDQNLPWEERKRVVNELVRQSFKPEFINRLDDIVVFAPLSTD
                     DLSQIVELYIDRLARRLGDRRLELAVTPDARTWLAERGYDPVYGARPLRRLMQREIDD
                     KLARALLSGEIRDGDTVLVGVADSSDGLTVARLEG"
     gene            17445..18494
                     /locus_tag="PLW15_01215"
     CDS             17445..18494
                     /locus_tag="PLW15_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOT33050.1"
                     /translation="MRATVIHGERDIRFEEVADPVLSTGGDAIVRVVAACVCGSDLWP
                     YRGITPTPRPKRIGHEFVGIVEAIGPDVLTISVGDFVIAPFYDCDMTCINCLNGVSTS
                     CLHGGWWGSDDRLGGFADGAQGERVRVPHADGSLVATPGAPAEHLVPSLLTLADVMGT
                     GHHAAVSAGVTHGSTVVVVGDGAVGLCAVLAAKRLGASRIVAMSRHADRQAVAREFGA
                     TDIVEERGAEGIARIKELFDGIGPDCVLECVGTKESMDQALRSARPGGMVGYVGVPNG
                     GAELPIGLMFGTNVGANGGVAPVRNYIDELLPEVLDGRLNPGRVFDLELPMTEVAEAY
                     AAMDERRAIKVLLRP"
     gene            complement(18498..18869)
                     /locus_tag="PLW15_01220"
     CDS             complement(18498..18869)
                     /locus_tag="PLW15_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33051.1"
                     /translation="MSNEGFVEYTPEGRPPRSWDFFLTIFLLLLLLILTGVFVIVGFG
                     LSVSALGCADSSSCNYLVISVGTLVVMIGTPVVALAGIIVSVVWIARRKRSWLVALIA
                     CLLSVGLFFLGTTLVRLAVPA"
     gene            complement(18866..19933)
                     /locus_tag="PLW15_01225"
     CDS             complement(18866..19933)
                     /locus_tag="PLW15_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOT33052.1"
                     /translation="MTQLHDPQLRGFASDNYAGVHPEVLAAIAEANGGHQIAYGEDVY
                     TTRLQEVMREYFGEAAEAFPVFNGTGANVTALTSVLPRWGAVVSTSTAHIHTDEGGAP
                     ERISGLKLLPVATPDGKLTPDLIGEEAWGWGDEHRAQPLAVSITQTTELGTLYSVDEV
                     RAIADYAHERGMVLHMDGARISNAAAALGVGFREFTTDAGVDILSFGGTKNGLLYGEA
                     IVVLNPSVSDGLKYLRKLNMQLASKMRFVSAQLIALLSDGLWLRSASHANAMAARLRA
                     GLEEEISTGSISGVNFTQETRANAVFVTLPPGVADRLRESFRFYDWNPATGEVRWMTA
                     FDTTEHDIDAFLAALRTELSV"
BASE COUNT         3298 a         6672 c         6897 g         2989 t
ORIGIN      
        1 cgtcatccgt cgttactcga cctcgtttgg ccttgcctcg cggctgctgg ggtcgggtgt
       61 gcgtcagcac gtggagaaca tctacgccct tgtgcgcgtg gccgacgaga tcgtcgacgg
      121 cggcgccgag gaggccggcg tcgacacgat cggcgccggg cgagcgctca acgagctcga
      181 gcgcgagacc gagaacgcga tggacaccgg cttcagctcc aacctcgtgg tgcacgcctt
      241 cgcgttgacc gcgcgcgaga cgagctttgg cgccgaactg acggctccgt tcttcgagtc
      301 gatgcgcatg gacctcagcg cgaagcagca cgatcaggcg agcttcgagc gctacgtcta
      361 cggctccgcc gaggtcgtgg gtctcatgtg cctgcgcgca ttcgtgctcg gcgagaaggt
      421 ggatgacctg cctcgcctcg agaccggcgc acgcgccctc ggggctgcgt tccagaaggt
      481 caacttcctg cgcgacctcg ccgccgactt cgagaccctc ggccgcagct actttccgaa
      541 cgtgaacgtc gagaccttca ccgaggagga gaaggtgcgt ctgctcgacg acatcgacgc
      601 cgacctcgcc gtctccgcgg ccgtgctgcc cgacctgccg gccagcagtc gtcgcgcggt
      661 ggccctcgcc cagtccctgt tcgccgagct ctcgcgtcgg ctgcgcaaga cgcccgccga
      721 ccagctcgtg cgctcccggg tgcgcgtgcc caaccccgtc aaggtcaggc tggcgctcgc
      781 cgcgagtgcc ggatcactgc cccgttcgaa aggtcgatca tgagtcgcgt cgtcgtcatc
      841 gggggtggca tcgcgggact cgcgagtgcc gccctgctcg ctcgcgaggg ccacgaggtg
      901 accctgctcg agggacgccc cgaggtcggc gggcgcgccg gcagctggga gcaggatggc
      961 ttccgcttcg acaccggccc gagctggtac ctcatgcccg aggtcttcga ccacttctac
     1021 aagctgctgg gaacctcggc cgacgagcag ctcaagctcg tcaccctcga ccccggctac
     1081 cgcgtctact tcgagggcgt cgccgagccg ctggatgtct ccgccgagct cgaggacaac
     1141 ctcgagatgt tcgaggccat cgagcccggc gccggcgatc ggcttgagaa gtacctggac
     1201 tccgcgcggg aaacgtacga actcgcgaag ctgcgttttc tgtacacgac cttcgcggac
     1261 atccgcaccc tgcttcgccg ggatgtgctc tcgaagaccg gcaagttcct gcgcctgctg
     1321 ctcgagccgc tgtcgagtct cgtggggcgc aagttccacg accagcgttt gcgccaggtg
     1381 ctcggctacc cggccgtgtt cctcggctcg tcgccgtacc tgaccccgag catgtaccac
     1441 ctcatgagcc acctcgacct ggccgacgga gtgctgtacc cgatcggcgg gttcacccgc
     1501 gtgatcgagt cgatcgccga cctcgctcgt gccgagggcg cgacgatcat cacccgcgcc
     1561 gaggtcacgc gcatcgtcgt ggagaatggc aaggcgaccg gcgtcgagta cctcgacgag
     1621 catcgggaga tccagttcgc cgacgccgac atcgtggtct cggccgccga cctgcaccac
     1681 agcgagaccg agctgctcaa gccggaggag cagacctacc cgcaggagta ctgggacacc
     1741 aagacccccg gtcccagcgc gctgctgctg tacctgggtg tcgagggcga gctgccgcag
     1801 ctggagcacc acacgctgct cttcacccgc gagtgggagc gcggcttcga cgccatcttc
     1861 ggaaccccct catcggtgcc cgagcccgcc tcgctctaca tctgcaagcc cggtggtgtc
     1921 gatccggatg tcgcacccga aggctacgag aacctgttcg tgctcgtgcc gatccccgcc
     1981 gatcccacga tcgggcgcgg cgacgtcgac ggtgacgggg acacccgcat cgaggtgctg
     2041 gccgaccggg tcatcgcgca gatcgccgac tggacgggca tcccggatct ggcctcgcgc
     2101 atcaccgtgc gccgcaccgt gggccccggc aacttcgccg acgagctgca cgcctggcgc
     2161 ggcaccgcgc tgggccccgc gcacacgctc aagcagagcg cgttcttccg ggccggcaac
     2221 gtgtcgaaga aggtcgacgg gctgtactac gtgggcggct cgacgatccc gggcatcgga
     2281 ctgccgatgt gcctgatcag cgcggagatc ctcatcaagc gactgcgtgg cgacacgtcg
     2341 accgagccga tggcggagcc gctggtgccg tcggcggtcg acgccccggt ggtcgagctt
     2401 gtcgagaccc cggcgcagat ctcgacaggc tcgatcaccg aagcaggctc gatcaccgaa
     2461 gctggctcga tcaccgaagc gcgatgagct ttctctacct cgctgccctt gcggtcgccc
     2521 tcaccggcat ggtgatgctc gaccgcaggt tccgactgtt cttctgggcg gcgccgtggc
     2581 gggcggcggt ggtgctggtg gtcggcctcg cgttcttcct cgcctgggat ctcgccgggg
     2641 tgggactcgg cgtgttcttc cgcggggaga cctcgttcat gaccggcctg caggtcgccc
     2701 ccgaggtgcc actggaggag gtcttcttcc tcacgctgct gtgctacctg acgatgaacg
     2761 cgtacgccgc ggcgtcgctg ctgctccagg cgcggtcgct gcggacacac gcgggtgctg
     2821 aggacgggcg agatgtccga aacgacagcg cgggcggcgc cgcatgacct actgggcgct
     2881 caacgtcgtg ttcctcgtgc cggtcgcggt gctcgtgatc gcgggactca tcgtgcgccg
     2941 caccccgcgc tggccggcgg tcgccctgac ggcggccgtg gtgctggtga tgacggcgat
     3001 cttcgacaac gtgatgatct ccgtcgggct tgtcgcctac aacccggccg cgatatccgg
     3061 ggcgttcgtg ggggtcgcgc cgctcgagga tttcgcctac gcggtggcgg cggtcgtggg
     3121 tctgcccgcg ctgtgggccc tgctcggcgg ccgccggtcg agtagcccga agggcgtatc
     3181 gagacccgcc cccgaggagg agcccaccga tgcttaaggc gctgttcgtg tcatcccggc
     3241 cactgagctg ggtgaacacc gcgttccctt tcgcggccgc ctacctgctg accaccggcc
     3301 aggtcgacgt gaccctgatc gtgggcacga tcttcttcct catcccgtac aacctcgcga
     3361 tgtacggcat caacgacgtc ttcgactacg agtccgatct gcgcaacccg cgcaagggcg
     3421 gtgtcgaggg tgcccttctc gacacccgga tgcaccggcc gaccttgatc gcctcggtcg
     3481 tgacgaccgt gccgttcgtc gtctacctcg tgatcgtggg gtcgcccctg tcgtggctgg
     3541 tgctggccat ctcgctgttc gccgtggtcg cctactcggc caagggactg cgcttcaagg
     3601 agaagccgtt cctcgattcg atcacctcga gcactcactt cgtctcgccc gcggtctacg
     3661 gcctcgtgct ggcgggtgcg gtgttcacga cgcaactgtg ggcggtgctc ggcgcgttct
     3721 tcctgtgggg catggcgtcg cacgcgttcg gtgcggtgca ggacgtcatc gccgaccgcg
     3781 agggcggcat cgcctcgatc gcgacggtga tcggcgcccg ctggacggtg cgcttcgccg
     3841 tgatcgccta cctcctcggc ggagtgctgc tgctgttcag cgcgtggccc gggcctctgg
     3901 cagcgatcct cgtgctgccg tacgccgcgg tttgcgcacc gttctggaac gtgacggatg
     3961 ccgcggccga caccgccaac cgcgggtggc ggcgattcct gctcctcaac ttcatcaccg
     4021 ggttcttcgt gacgatgctc atgatctggt gggcgttcac cgcccgctga cgacgaactg
     4081 ccgcacctcc tcgacgaccg cggggtcgct gaggatgcgc aggtggccga gtcccgaggt
     4141 gatcacggag tgcaccggcc cggtgtgcgc gttggcgatg agcagtgcct ggccgggatc
     4201 gacggtcgcg tcatccgtgt cgtgcaccag gagcacgggc acgtgggtgg ccgacgggtc
     4261 cagttcggcg acgaaacggc tccaggggtc gacgacctcg gggaacgtgc ggcgttcgat
     4321 ccgtcgccgc atgccgcgca cggctcgggc gcccaggccg gcgagggcgc agaacttgtc
     4381 gacgagctgg tcggcgttcg ccatccccga gattccgacg atgcgcttga cggtcacgcc
     4441 ctcgcgcacg gcgacgaagg tcgcgagcac cccgaacgag tgcgccacga tcgtgtcgat
     4501 cgggccgtgc cgcagcgaca gggcctcgat cgcggcggcg tagtcgagga tcgtcacgag
     4561 ccgaccgggt gagtcgccat tcgcgggcgc gtcgaaggcg atgacggtga cgtcggctgc
     4621 ctgcagcgcg gcgacgagtg ccgagaatcg ggatgcccgg ctccgccacc cgtgcacgag
     4681 aagtatcacg cgctcgcccg acccccaccg gtaggtcatg atctcgcggc cgccggcatc
     4741 cacgacctcg cggtacgccc acttgtgcac cagcgagtcg cgctcgtgca cgggctgtgg
     4801 cgtgccgacg tggcgccaca accgccaccc cacctcggct cccagtgcgg gagccacgcg
     4861 gctgagggtg gcgagggcga cgcgggcggc gggcatccgg agtcctttcg tgtttaaata
     4921 cgatcgttcg tatagtatcg aaaggcggga ggaacgcaac aatggatgcc atgaccacgg
     4981 ccgccgacgg acgacgagcg cgcggggacg agtcgcgccg ccaggtgatg gagcgagccg
     5041 tcaacctcgc gtcagtcggc gggctcgacg ggctcaccat cggtgccctg gcaatcgaga
     5101 ccgaccggag caagggcggt gtcgtcgccc tgttcggcac gaaggagcag ttgcagcttg
     5161 cgacagtcga ggccgcgcgc gagatcttca tcgagcgggt gatcgcgccg gcgctcaccg
     5221 tgcccggcgg cctcgatcga ctgcgcgcgc tgctcgacaa ctggctcgac tacagccagg
     5281 gccgggtgtt cgccggtgga tgcttcttcg ccgcagccgc catggaggtc ggggcccagc
     5341 ccgggcccgt tcgcgatgcc gtcgccgatg ccatggccga ctggtacgag accgtgcgcc
     5401 gcatcgccca gcgagccatc gatgcggggg agttggcgcc gggcacggat gccgcccaac
     5461 tcgccttcga ggtcacggcg atcctcgagg gcgcgaactc ggcgtcgcgg ctcaccgaca
     5521 gcgtggagcc ctacgcgcgg gcgcgggtcg cgctggccgg gttggttcag ccggcgtagc
     5581 cgaggttgcg ggccatgcgc tcgagcacgg cgacggcctg cccgtactgg tcggccgtga
     5641 tgccctccgc gacctgggtg cgctgagcgg cgaccacgag ttcgagccgc tcgaaggcgc
     5701 cgcgtccgcg gtcggtgagc gcgtagccct gcgcggtgtc ctccagccag gcgctgtcca
     5761 gcagctcggc gaggtggtcg gcggaggtct cggcggggtc gtcgtcggtc gcgcccgaca
     5821 ggaacggcgc caccgcggca tccagttgct ccaccgtcgc gggcccttgt gtgagcacgt
     5881 tcaggagttg ccactggcgt cgggtgacgc cgtgttcctc gagtgtcgag gcgaactgct
     5941 cgtcgatcag tcggtcaacg agcttgagcc agaaacctat gggtcgagga gtgtccatgc
     6001 ttcgaggtta cgcctcgggc tccgcggcgg gcgaggagtc ctgtgcattc tcaggacggt
     6061 ggtgcgggtg gtcgtggtga ccgtgaccgt ggccgggtcc gaagccaggg ccgaagccac
     6121 gcccgaagcc gcgcttgggg ccgcgaccga agccggggcc gaagccgcga ccgcgcgggc
     6181 cgaagtcgtc gtcctcgctc cagcccaggt tgagcgccat cttctcgagg gtggccatgg
     6241 tggtggcgta gtcctcgtcg gagatgccct cgcgggcctt gtcgttcagc gtgtcgcggt
     6301 attcgcgacc cagctggtgc ttgcggaact cgatcgcccg gagccagtgg ctggggcggg
     6361 gggcagttgt ttcgttgggg gtgttcatgg ggttcttctt tcggattggt tttacatgtc
     6421 atatgacaat gaatgtatag ttacatacat gtcttcggaa agtcaagcgg atgccattcg
     6481 ccagatcgag caggccctcc tggccctgcg cacgggcggt cgacgccacg gcggaccgcg
     6541 cggcgccggc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtcaccac
     6661 ggaccccccg gactcgcggg cgcggcccgc ttccgcatgc tcgaggtgct cgagtcagcg
     6721 gctcctgaga cgcgcatggg catctccgag gtggcggccg cgatcggcgt cgatcagccc
     6781 cgcgccagcc ggctggtcaa cgatgcggcc gagcgcggcc tggtgctgcg atccgccgac
     6841 ccgcgggatg cccgccgcag cgtcatcgcg ctgaccgacg ccggccgggc gctcctggat
     6901 tcggcacggg cattccgccg cagtgcggtc acggaagcgc tcgccgactt caccgacgac
     6961 gagacgcagg cgctcgccgc actactcacg cgtctcgcgg cggcttggcc gcaggcgccg
     7021 cgctagccgc gaccgctcag tacgagaagc cgagctcggc ggggtactcg ccgagggcgg
     7081 tgagctgcgt cgccgcagcc tgcaggtgcg cgagcgtcaa cccgagagtg cccggcccga
     7141 agctcacccg cgacaccccc agctcggcga gggtcgtgag cggcggagcg ctggggttgg
     7201 cgatcaccga tacgcgtccg tcgatgtgct cgatggccca cgagatcgac tcctcgtcgc
     7261 gcacgcccag catgaagatc acgtcggccc ccgcggcgag gtaggcgttc gcgcgagcga
     7321 ttgcgtcgtc ccgggttccg ccgcgcacga gcgcatcgac ccgggcgttg agcacgatcg
     7381 ggatgcccgt ggcctccccc gcgcgccgca ccgccgccac gcgctccacc gccagctcgt
     7441 gcgggaacag cgcgtcgggt ccgctgctgt cctcgaggtt gagccccacg gtgccggtcg
     7501 cgatgagctg ctcgacgtgg tgctgcactg ccgcggcatc cgtcgcgtag ccgcgctcga
     7561 agtcgacgga gaccggcagc tcgactgcgg cgacgacccg gcgggccgca tccagtgcct
     7621 cgtcgagggt gagcgcctcg ccgtcgtcga ccccgtgcgc atagctgacg gcgtggctgg
     7681 cggtggcgat cgcgcgcacg ccgggggcgg cagccacgat gcgcgcggtc acggcatccc
     7741 agacattggt gacgatgagc gggtcgccgg gaacatgcag cgagtcgagc agtcgggcct
     7801 tgtccagttg ggtcatgccc tcagcctgac gggtggatgc tgccaccgcc agcgactttc
     7861 ggacagcagc gtcagcgcac ccgcacgtgc ttggggatct tcacgaagag cagcgcgaca
     7921 gcaccgacca gcagcacgag cgcgataccc agcacaccgt agatcgtcgc gccgaaggcg
     7981 acgatgaaca gggtccagag cgcgggggag atgaagctgg ccacccggcc ggtcgtcgcg
     8041 tacaggccga acacctcgcc ctgtcgaccc tcgggcgtca cgcgggccag gagcgagcgg
     8101 ctggccgcct gcgcggggcc gacgaacgcg gagaggatga gtccgcccgc ccagaagacg
     8161 gcagtgccgg tgtcatgaag cgcgaagatc accacggcca tggcaaccag gccgaacagg
     8221 gcgaacagga tgacggcctt cgggccgacc atgtcgtcga tgcggcccgc gatgatcgtg
     8281 gagattccgg cgacgatgtt ggccgcgatg ccgaagatca tgacttcgtt ggcagtgaag
     8341 ccgaacgcca cggcggcgag gatgccgccg aaggcgaaga ctccggcgag cccgtcgcgg
     8401 tagatcgcgc tggcgatgag gaaccacccg gtcgacgccg actccttgaa gagcttgccg
     8461 atgtccttga ccagcacgac gtagctctgg aagaagccga tcttcgcgat gtcgggccgc
     8521 gcggccggag cctcgggaac gttgaagaag agcggcagcg agaacaggat cgtccagacg
     8581 cccgcgccga cggcgatgag gcggtacgcg agcccgttgg aggtgtccag accgaaccag
     8641 tccgcgaagg tcaggatgac gacgatgacc agagcgacga ttccgccgat gtagccaagg
     8701 ccccagccga gaccgctgac cttgccgatc gtcttgcgtg tcgacacctg cacgagcatc
     8761 gcgttgtagt tgaccccggc gatctcggag acgacggcac cgagcgcgag gaggccggcg
     8821 ccgagccaga agtaggcggg ctgggcgaag acgaagaaca gcccgaactg gatcagcgcg
     8881 aggatgccgg tgaacagcag caggaggcgc ttctgcatgc ccgaggcatc cgctcgctgg
     8941 ccgagcaccg gggcgagcag gaagatcagc acaccggcca gcgtggtggc gagtccgtaa
     9001 ccggccgtga gacccgcgag cgcctgctcc ttgacggcat caccgtcggg gagggcggcg
     9061 atgtccgccg gcaggaagtt gtcggagacg aggtacagcg aggcgaagac gaaggtgagg
     9121 atcaccgagt tgaacggctg agtcgcccag tcccacatcg cccaggagat cacctgcttg
     9181 cgcggaacca ccttgtcgtc atcggcgacc ctgccgaccg cgcccaccgc aagggtgtcg
     9241 gcggcgaccg ggatcttcga gtcggtgggc ttcacagcgt cgggtagctc gtccgtcatg
     9301 ctcacacgct agtggctgga ggtgttctcc acgtgaactt ttctgaccca accctgagcg
     9361 gtggtgtgaa tgagcacgag cgtgatcgcg gccagcaccg agttgcggaa tgccgggtcg
     9421 agaccgttcc acgcggtgga gcgccacatc tggaaccact ccccgccgac ggtgatgaat
     9481 ccgccgaaga acagcacgat gatcatgagc aggccgatgc tcgaccagat gcgtgcggag
     9541 tcgaacgttc cccgggtgat gagggctcgc agccacagca cacaggcccc gatgagcacg
     9601 atcgctgcga ggctctccca gatgatcagc ccgatgtacg ccacgttcca caccacggga
     9661 tcggtgatcg cgcgccacat gacatccggg tcgagcccgg tgccgtcggc cgccccgaag
     9721 ttggtggtgt ccatcgagag cacgtgcttg acgaactcga agttggtgcc gtagtcggtg
     9781 atgttaccga gcgccaccag gaagatgtag agcgcgttgc acgctaccag cacggcgacg
     9841 gcgaagggca gcgagccgag ccgctgccac cagacggggt tctcagaggt cgaggtcacg
     9901 tgtgcactgt aggccccgag cggatgccgc gtcaccttga gccgcctcga ctcaacttcg
     9961 acttgacaga agttatccac aggttgcaaa cttgatacat cgcggctcag gtttgggagc
    10021 gcgagcagac cacaagcacc acaagcaagg agatagagca catggctcga gcagtaggaa
    10081 tcgacctggg aaccaccaac tcggtggtcg cagttctcga aggtggagaa cccaccgtca
    10141 tcgccaacgc cgaaggcttc cgcaccaccc cctcggtggt ggccttcacc aaggacggtg
    10201 agacgctcgt cggtgagacc gccaagcgcc aggccgtcac caacgtcgac cgcaccatcg
    10261 cctccgtcaa gcgccacatg ggcacggact ggaagcagga cgtcgacagc aagaagtaca
    10321 ccgcgcagga gatctccgcg cgcatcctcg ccaagctcaa gcgtgacgct gagcagtacc
    10381 tcggcgagcc ggtgaccgac gcggtcatca cggttcccgc gtacttcaac gacgccgagc
    10441 gccaggccac caaggaggcc ggcgagatcg cgggcctcaa cgtgctgcgc atcatcaacg
    10501 agccgaccgc ggctgcgctc gcctacggcc tcgacaaggg caaggaggac gagctcatcc
    10561 tcgtcttcga cctcggtggt ggaaccttcg acgtctccct cctcgaggtg ggcaaggacg
    10621 acgacttctc gaccattcag gtcaaggcca ccgcaggtga caaccgcctc ggtggcgacg
    10681 actgggatgc ccgcgtcgtc gactacctga tcaagcagtt caaggccacg accggcgttg
    10741 acgtctcggg cgacaagatc gccaagcagc gtctgaagga ggccgcggag caggcgaaga
    10801 aggagctgtc gagccagacc tcgaccacca tccagctgcc ctacctctcc ctcacggaga
    10861 acggccccgc caacctcgac gagacgctca cgcgcgccaa gttcgaggaa ctgaccgccg
    10921 acctgctcga gcgcaccaag aagccgttca acgacgtcat caaggaagcc ggagtcaagc
    10981 tgtcggatgt cgcacacgtc gtgctcgtgg gtggttccac ccgcatgccc gccgtcgtgg
    11041 atctcgtgaa gaagctcacc ggtggtcagg agcccaacaa gggcgtcaac cccgacgagg
    11101 tcgtggccgt gggcgctgcc ctgcaggccg gcgtgctgaa gggcgagcgc aaggacgttc
    11161 tgctcatcga cgtgaccccc ctgagcctcg gtatcgagac caagggcggc atcatgacga
    11221 agctcatcga gcgcaacacc gccatcccca ccaagcgcag tgagacgttc accacggccg
    11281 acgacaacca gccgtccgtg gcgatccagg tcttccaggg cgagcgcgag ttcacccggg
    11341 acaacaagaa cctgggcacg ttcgagctca ccggcatcgc acccgcgccc cgcggcatcc
    11401 cgcaggtcga ggtcaccttc gacatcgacg cgaacggcat cgtgcacgtg tctgccaagg
    11461 acaagggcac cggcaaggag cagtcgatga cgatcaccgg aggcagttcg ctgtcgaagg
    11521 aggacatcga gcgcatggtg cgcgagggtg aggagcacgc cgccgaggac aaggcacgcc
    11581 gcgaggccgc cgagacccgc aacacggctg agcagctcgc ctactcgacc gagaagctga
    11641 gcaaggacaa cgaggacaag ctgcccgccg acgtcaagac cgacgtgcag gctgatgtgg
    11701 atgccctcaa gtccgctctc gccggtgacg acgaggccgc ggtgaagtcc gcgttcgaca
    11761 ccctcaccgc gagccagcag aagctcggcg aggcgatcta cgcatccgga caggccgcgc
    11821 ctgccgacgg cgagaccgcc gagcccgcga ccgagggcag cgacgaggac atcgtcgatg
    11881 ccgaggttgt cgacgacgag cccgaggacg gcaagaagta gcccatgtct gacaacaacg
    11941 acgaacgaca cgagaacgag gagccggtca tccgcgacaa gcggaggatc gatcccgaga
    12001 ccggcgatgt tcgcgagcct gacgctgcgg cggtggggga tgacaccccc gccgccgagg
    12061 cggtcgacgc cgatggcgcc gacgctgccg aggagctgag cgacgccgac caggcgctgc
    12121 tcgaccaggc agcacaggac ctcgtcgccg agatgcgctc cgacatgctg cgcgcacagg
    12181 ccgagctggt gaacttccgc acccgtgtgg aacgtgaccg ggcggccaac cgcgaagcag
    12241 tgatcgccga ggtcatccgc tcgctgctgc ccgcactcga cgatctggcc cgggccgagg
    12301 cgcacggtga tctcatcgag ggcgctccgc tcacgatcat cgcccagaag ttgcggggcg
    12361 ctttcgagaa gtacggattg cgtcagatcg gcgagaaggg cgaagccttc gaccccgcct
    12421 tccacgaagc agtcgtgcag ttgcccagcc cggatgtcac ggtcaacacc atcgcggacg
    12481 tcatcgagcc cggctatgtt ttgggggacc gcctcgtgcg gcccgcgaag gtggccgtgt
    12541 cggttccggc cgagtaagag gagtcaccat ggccagtcag gactggttcg acaaggactt
    12601 ctacgccgtg ctcggagtat cgaaggatgt ctccgaggcg gacctcaaga agacctaccg
    12661 caagctcgcg cgccagtatc acccggactc caacccgggg gatgcgaagg ccgaagccaa
    12721 gttcaaggag atcagcgagg cccactcggt gctctccgac ccggagatgc gtcgtgagta
    12781 cgaccagatc cgggcgatgg gctccggcgc gcggttcgcg cccagcagcg gtgccaacgg
    12841 cgggttcgag gatgtcttcg gtggcatgtt cggaaaccgc ggcacctacc agggcacgag
    12901 cttcgaggac ctcctgggcg gcatgttcgg cggctcgaat cagggcttcc gcaccggccc
    12961 cacggccggt cgggatgtga ccgcgtcgac gaccctggac ttcgtgacgg ccatcaaggg
    13021 cgagacgatc aagctccagc ccgccggcgg cgccccgatc aacgtcaaga tccctgcggg
    13081 ggtcagcgac ggccagaaga tcaagctgaa gggcaagggc gagccgagcc acgacggcgg
    13141 tgcgcccggc gacctggtgc tcacggtcac ggtgcgcaag cacccggtgt tcgagcgcga
    13201 cggcctgaac cttcgagtcg aggtgccggt gaccttcgcc gaggccgcgc tcggcgcgac
    13261 catcgaggtg ccgacgctgg gcggcgagcc ggtgaaactg cgggtcgccg cgggaacgcc
    13321 cggcgggcgc atccttcggg tcaagggccg aggggtcacg acagccaagg gcaccggcga
    13381 cctgctcgcc gaggtgcagg tggcggttcc gggcagggtt ccggatgacg ctgccgctca
    13441 ccttcgcgcg ttcgaggcgg ccatgccgca ggagaacccg cgggccgagt tgctcgacaa
    13501 agcgaggagt gaccactgat gtcagcgccg ctcgatgagt tctcgccggt cttcgcgatc
    13561 gcggttgccg ccgagctcgc gggcatgcac ccgcagaccc tgcgtcagta cgaccgcctc
    13621 ggactcgtca gccccacccg cacggcgggg cagtcacgcc gctactcgat gcgggatgtc
    13681 gcacagctgc gcgagatcgc gtcactgagc gcacagggtc tcaacctcga gggaatcaag
    13741 cgcatcctcg agctggagaa ccaggtggca gcgctggcgg gccgcgtgcg agagctcgaa
    13801 cacgccctcg cggacgagct tctcgcccga ccgggacgac gcgtcttcgc ggccggcgga
    13861 tcgggcgagg tcgtgtcgat caaggcgggt tcgcgagtgg cgaagtccaa ccaggtcgtc
    13921 gtgtggcgac cgctgcgccg aggctgagcg acatggccgg ggagcagctc gacacgatcg
    13981 acgaccgggt gctctcgggg ctgcgccgct ggccggacgt cgaggcgccg aacctgcacg
    14041 cggtcgacgc gagcgaccga ctcattctcg atgaggcggc cgcggcgatc gccgagtccg
    14101 ggcccggcga agttgttgtg atcggggacg cgtacggtgc gctgaccatc ggtgccgccg
    14161 ctatcggcgc gacgggcatc cgggtgttcc aggattcgat cgtgggagag cgggcactcg
    14221 cggccaacgc cgccggtgcc gtcgactacg tgaccctgcc cgtcggcgcc gacctgctgc
    14281 gcggcgcacg cgtcgtgctg atgcagctgc cgcgctcgct ggccgagctc gacgagctcg
    14341 cggcgtggat cgcgtcggcc gccgaccctg cggtgcgggt atatgccggc ggacgcatca
    14401 agcacatgac gacggcgatg aacgaggtgc tcgggcgcca cttcgggcga gtggatgtca
    14461 cgcacgcccg tcagaagtcg cgcgtgctga tcgcatcgca gccactgccg agtgagccgc
    14521 cgatgatcgc gcgggagttc cataccgatc tcggcatatg ggtgtgcgcc accggcgcga
    14581 ccttcgcggg cacccgcatc gacatcggga cgcgcgcgct gctcgcggtg ctgcccactg
    14641 tggctccgga tgccacaacc gcagcagacc tcggctgcgg ctccggcgtg gtcgccgccg
    14701 ttcttgcgca ggcacggccc gagctggctg tgctggccag cgacgtctcg gcgggcgccg
    14761 tggcatccgc tcgggcgacg gccgaagcca acgcgctcac gaacgtgacg gtgacccggg
    14821 acgatgccct gggtggccag cccgacgcga gtctcgacct ggtggtgctg aacccgccgt
    14881 tccacctcgg ggcgaccgtg cacacgggcg cggcctcgaa gctgttcgcc gccgcggctc
    14941 gcgtgctgaa gcagggcggc gaactcgtca ccgtctacaa ctcgcacctc ggctaccgag
    15001 ccgagctgac cagagtcgtc gggccgaccg acgaactctc ccgcacctcc aaattcaccg
    15061 tcacacgatc aaggaagaac tgatggccaa catgcagggt gcccccggca ccgacgaaga
    15121 gcagaagacc gcgcttcagc agtacggcgt gaatctcacc gacatcgcgc gcagcggcaa
    15181 gctcgacccg gtgatcgggc gtgacgccga gatccgccgc gtgagccagg tgctgactcg
    15241 ccgcaccaag aacaaccccg tgctcatcgg cgagcccggc gtcggcaaga ccgccgtcgt
    15301 cgagggactc gcccagcgca tcgtcgccgg cgacgtcgcc gactcgctca aggacaagca
    15361 gctcgtgtcg ctggacctcg ccgcactcgt ggccggcgcg aagtaccgcg gcgagttcga
    15421 ggagcgactc aaggccgtgc tcaaggagat caacgactcc gacgggcaga tcatcacctt
    15481 catcgacgag ctgcacacgc tcatgggcgc gggcggcggc gagggctcgg tcgcggcagc
    15541 caacatgctg aagcccatgc tcgcccgcgg cgagctgcgc ctcatcggtg cgaccacgct
    15601 cgacgagtac cgccagtaca tcgagaagga tgccgcgctg gagcgccgtt tccagcaggt
    15661 gttcgtgggc gagccgagcg ttgaggacac cgtcgcgatc ctgcgcggac tcaaggagcg
    15721 ctacgaggcg caccacaagg tgacgatcgc cgactccgcg ctggtcgcgg cggcgacgct
    15781 cagcaaccgg tacatcaccg gacgcaagct gcccgacaag gccatcgacc tgatcgacga
    15841 ggccgcgtca cggctgaaga tggagatcga ctccagcccg accgagatcg accagctcaa
    15901 gcgcgccgtc gaccggctgc gcatcgagga gctcgcgctc aagaaggaga aggacgacgc
    15961 gtcgaaggct cgcctcgaga agctgcggga ggacctcggc gaacgccaga aggagctcga
    16021 cgcactggag gcacgctgga aggcggagaa ggcatccctc accggtgtcg gcgacctgaa
    16081 gacccagctc aacgacgctc gcatcgacct cgaccgcgcg atgcgcgagg gcgactacca
    16141 gaaggcgtcc aagctcaact acgagacgat tcccaagatc gaggccgcgc tggtgaccgc
    16201 ggagtcctcc gagccggtcg gcccgcgcat ggtcaacgat caggtcaccg aggaggacat
    16261 cgccagcgtg gtggcggcgt ggacgggcat cccggtcgat cgcctcaccc agggtgagac
    16321 cgagaagctg ctgcacctcg aggccgaact gggcaagcgc ctgatcggcc agaaggatgc
    16381 cgtcgctgcg gtctccgagg cggtgcgccg cacgcgtgcc ggcatctccg accccgaccg
    16441 gccgaccggc tcgttcctgt tcctcgggcc gaccggtgtc ggcaagacgg agctcgcgaa
    16501 ggcgctcgcc gagtacctgt tcgacgacga gaaggccctc gtgcgcatcg acatgagcga
    16561 gtacggcgag aagttctccg tgtcccggct cgtcggtgcc cctcccgggt acatcggcta
    16621 cgagcagggc ggccagctga ctgaagcggt gcggcgtcgg ccgtactcgg tgatcctgct
    16681 cgacgaggtc gagaaggcgc atccggaggt cttcgacatc ctgctccagg tgctggatga
    16741 cggtcgcctc accgacgggc aggggcgcac ggtcgacttc cgcaacgtga tcctgatcct
    16801 cacgtcgaac ctcggcagcc agttcatcac cgaccagaac ctcccctggg aggagcgcaa
    16861 gcgcgttgtc aacgagctcg tgcgccagtc gttcaagccc gagttcatca accgtctcga
    16921 cgacatcgtg gtgttcgcgc cgctgtcgac cgatgacctc agccagatcg tcgagttgta
    16981 catcgaccgc ctcgcccgtc gcctggggga tcgtcgcctc gagctggcgg tgaccccgga
    17041 tgcccgcacc tggctcgccg agcgcggcta cgacccggtg tacggtgcgc gcccgctgcg
    17101 gcggctcatg cagcgcgaga tcgacgacaa gctcgctcgc gcgctgctgt ccggcgagat
    17161 ccgcgacggc gacaccgtgc tcgtgggtgt ggcggacagc agtgacgggc tgaccgtcgc
    17221 gcgactcgag ggctgaggcg cgctctcgcg gatcaggaga ttgcagcaac acgccgcggc
    17281 gcccgctcat cgtgacggag tgttgtccgg atctcctgat ccgcgaatgg gagccgggcg
    17341 ggagccgggc gtcgaggtgg gcgggagccg ggcgtcgagg tgggcgggag ccgggcgccg
    17401 aggtgggcgg atcgcgggct caccggcgcg cgtaggctgg aaccatgcgc gcaacggtga
    17461 ttcacgggga acgagacatc cgcttcgagg aggtcgccga cccggtgctg agcaccggcg
    17521 gagacgcgat cgtgcgggtt gtcgctgcgt gcgtgtgcgg ctctgacctg tggccgtacc
    17581 gcggcatcac cccgaccccg cgccccaagc gcatcgggca cgagttcgtg gggatcgtcg
    17641 aggcgatcgg ccccgacgtg ctcaccatct cggtcggcga cttcgtgatc gcgcccttct
    17701 acgactgcga catgacctgc atcaactgtc tcaacggcgt gagcacctcg tgtctgcacg
    17761 gcggctggtg gggctcggat gaccgcctcg gcggcttcgc ggacggcgcg cagggcgagc
    17821 gggtgcgtgt gccccacgcc gacggatcac tcgtggcgac acccggagcg ccggccgagc
    17881 acctcgtgcc gagcctgctc acgctcgcgg acgtgatggg caccggccac cacgccgcgg
    17941 tgtccgctgg cgtgacgcac ggctccaccg ttgtggtggt gggcgacggt gcggtcgggc
    18001 tctgcgcggt gctggccgcg aagcggctcg gggcatcccg gatcgtggcc atgtcgcggc
    18061 acgccgaccg gcaggccgtc gcgcgcgagt tcggcgcgac cgacatcgtc gaggagcgcg
    18121 gcgcggaggg catcgcgcgc atcaaggagc tgttcgacgg aatcggcccc gactgcgtac
    18181 tcgagtgcgt cggcacgaag gagtcgatgg accaggcgct gcggtcggcc cgtccgggcg
    18241 ggatggtcgg ctacgtgggc gtgccgaatg gcggcgctga gttgccgatc ggcctgatgt
    18301 tcggcaccaa cgtcggagcc aacggcgggg ttgcaccggt gcgcaactac atcgacgagc
    18361 tgctgcccga ggtgctcgac ggccggctca acccgggtcg cgtcttcgac ctcgagttgc
    18421 cgatgaccga ggtcgcggag gcgtacgcgg ccatggacga gcgccgcgcg atcaaggtgc
    18481 tgctgcgccc ctaggtgcta ggcgggaacc gccaggcgca ccagggtggt gcccaggaag
    18541 aacagcccga ccgacagcag gcaggcgatg agagccacca gccacgagcg tttgcgtcgc
    18601 gcgatccaca cgacggacac gatgattccg gcgagggcaa cgaccggcgt gccgatcatc
    18661 acgacgaggg tacccacgct gatcacgagg tagttgcacg acgacgaatc cgcgcagccc
    18721 aaggcggaga cgctcagccc gaagccgacg atcacgaaga cgcccgtgag gatcagcaga
    18781 agcagcagca ggaagatcgt gaggaagaag tcccaactgc gcgggggacg accctccggg
    18841 gtgtactcga caaagccttc gttgctcata ctgatagctc cgttctcagg gccgcgagga
    18901 acgcgtcgat gtcgtgctcg gtcgtgtcga acgctgtcat ccagcgcact tcgccggtgg
    18961 ccgggttcca gtcgtagaac cggaagctct cgcgcagcct atctgccacg ccgggaggca
    19021 gggtcacgaa caccgcgttg gcccgggtct cctgcgtgaa gttcactccg gagatcgagc
    19081 ccgtcgagat ctcttcctca agccccgccc taagccgggc ggccatcgcg ttcgcatgag
    19141 aggcactgcg cagccacagc ccgtcggaga gcagcgcgat cagctgggct gagacgaagc
    19201 gcatcttcga cgcgagctgc atgttgagct tgcgcaggta cttcagtccg tcggagacgg
    19261 acgggttcag aaccacgatc gcctcgccgt agagcaggcc gttcttggtg ccgccgaagc
    19321 tcaggatgtc gaccccggca tccgtggtga actcccggaa gccgacgccc agggccgcag
    19381 ccgcgttgga gatgcgcgcg ccatccatgt gcagcaccat gccgcgctcg tgggcgtagt
    19441 cggcgatcgc ccgcacctcg tcgacgctgt acagggttcc gagttcggtg gtctgcgtga
    19501 tgctcacggc gagcggctgg gcgcggtgct cgtcgcccca gccccacgcc tcctcgccga
    19561 tgaggtcggg ggtgagcttg ccgtcgggcg tggcaacggg cagcagcttg aggccgctga
    19621 tgcgctcggg ggctccgccc tcgtcggtgt ggatgtgcgc ggtggaggtc gagacgaccg
    19681 caccccagcg cggcagcacg ctggtcaggg ccgtgacgtt ggcgccggtg ccgttgaaca
    19741 cggggaacgc ctcggcggct tcgccgaagt actcgcgcat gacctcctgg agacgggtgg
    19801 tgtagacgtc ctcgccgtag gcgatctggt ggccaccatt ggcctcggcg atcgccgcga
    19861 gtacctccgg gtgaacgccg gcgtaattgt cactggcaaa cccgcgcagc tggggatcgt
    19921 ggagttgcgt cactccacca gtctgtcagg tgcagg
//
LOCUS       DAOSOU010000011        19190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2871_length_19190_cov_6.242435, whole genome shotgun sequence.
ACCESSION   DAOSOU010000011 DAOSOU010000000
VERSION     DAOSOU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 19190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19190
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2871_length_19190_cov_6.242435"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLW15_01230"
     CDS             <1..620
                     /locus_tag="PLW15_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOT33053.1"
                     /translation="TLGVSWGRTMDAIAGHLVAGWATAVTVVQINGAVSLNRRPTTAA
                     NTAATIAQKAGGQVILLPSPAILERLETKRAIESDRTVAGVIELAAGASAYLFSGGVA
                     DGTSALVESGYLSGTDVTELRRKGAIGDVVGRFIDANGNIVDPGLDERTMGLSLEQLR
                     AAANAIFVVSGEAKHPVARAVVTSGLCTVIVTDEATALALLEENA"
     gene            617..1624
                     /gene="deoC"
                     /locus_tag="PLW15_01235"
     CDS             617..1624
                     /gene="deoC"
                     /locus_tag="PLW15_01235"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT33054.1"
                     /translation="MTPSAALAPAERALGLIGPDLTEKSLRLHLEGLSGIDAVGLEER
                     AAALGTRSIKTTSKAWALDTIIRLIDLTTLEGADTDGKVRSLVAKALNPDASDPTVPR
                     VAAVCVYGDMVPTAVDALGSAHGDPDDGNISVAAVATAFPSGRASLAVKTLDTAEAVA
                     AGADEIDMVIDRGAFLSGNYGLVFDQIVAVKEACRREDGSYAHLKVILETGELNTYDN
                     VRRASWLAILAGGDFIKTSTGKVAPAATLPVTLLMLEVVRDWHRLTGERIGVKPAGGI
                     RTSKDAIKYLVTVAETVGEEWLQPHLFRFGASSLLNDVLLQRQKLTTGRYSGPDYVTI
                     D"
     gene            1630..3093
                     /locus_tag="PLW15_01240"
     CDS             1630..3093
                     /locus_tag="PLW15_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT33055.1"
                     /translation="MTEIVKPHRFLDYAPAPESTSILHLKKSYGLFIDGEFVKGHGKP
                     FLTVAPATEEKIAEIANANSKDVDAAVAAARRAYDRTWSKLSGADRGKYLFRIARLVQ
                     ERARELAVAESIDNGKPIKESRDVDVPLVAAWFFYYAGWADKLDYAGLGPNPRALGVA
                     AQVIPWNFPLLMLAWKIAPALAAGNTVVIKPAETTPLSSLILAEILQQAELPAGVVNI
                     ITGAGDTGQALVNHPDVNKVAFTGSTAVGRAIAKSVAGSGKKLTLELGGKAANIVFDD
                     APIDQAVEGIVNGIFFNQGHVCCAGSRLLVQENVHDEVVDRLKLRLSTLRLGDPLDKN
                     TDIGAINSREQLERIRELSDIGEREGAERWTAPCVIPDKGFWFAPTIFTNVSTSSTIA
                     REEIFGPVLSVLTFRTPAEAIAKANNTPYGLSAGIWSEKGSRILAVADQLRAGVIWAN
                     TFNRFDPASPFGGYKESGYGREGGRHGLAAYLQGASW"
     gene            3087..3947
                     /locus_tag="PLW15_01245"
     CDS             3087..3947
                     /locus_tag="PLW15_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT33056.1"
                     /translation="MVGRLAVPKTYKLYIGGKFPRSESGRTYEVLSKKGAFLANAALA
                     SRKDARDAVVAARAAFGGWSSATAYNRGQVLYRIAELLEGRRDQFVDELVSAEGLSRT
                     AAVKQVDTAIDAWVWYAGWADKYVQVAGNGNAVSGPYFNLSTPEPTGVVAIVAPQSGG
                     SLLGLVSVVAPAIVSGNTVVVVAQQSAPLSAISLSEVLATSDVPGGVVNILTGSPAEI
                     APWLAGHADVNAIDLAGAGDLEWVDMQITAADTLKRVIAPEVGAPAPSLHRIVALTET
                     KTVWHTKSLI"
     gene            4034..5503
                     /locus_tag="PLW15_01250"
     CDS             4034..5503
                     /locus_tag="PLW15_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT33057.1"
                     /translation="MRILVTGATGYIGGRLAPRLVDAGHEVRILVRDAARVRDVPWAK
                     RVEVVTGDLSDAATLPAAFEGIDVAYYLVHAMGAGGDFERTELTSARNVAAAAKAAGV
                     KRIVYLGGLHPDVPLAQLSTHLRSRAEVGEILIASGVPTAALQAGTVIGSGSASFEMI
                     RHLTEVLPYMPAPKWVRNFIQPIAVRDVLYYLVAAASLPAEVNRTFDIGGPDVLRYGQ
                     MMNGYALEAGLRQRPIASLPVLTPWLASQWVNLVTPIPRQLAVPIIASLQYDCVASEH
                     DIAHYIPDPEGGLTGYRKAVRLALAKVRDGQVETSWQNAAIAGAPSNALPSDPDWSGH
                     TVYTDVREQHSAASVDQLWEVVESIGGENGWYSFPLAWAVRGWLDKLVGGVGLRRGRR
                     DARRLEPGDALDFWRVEDIDRGHFLRLRAEMKVPGRAWLEMTTEADPQGGSTYRQRAV
                     FFPSGLGGRLYWYSILPFHGVIFSGMANQITKSAERPVR"
     gene            5508..6389
                     /locus_tag="PLW15_01255"
     CDS             5508..6389
                     /locus_tag="PLW15_01255"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOT33058.1"
                     /translation="MSSVIVVGSANVDQVFRVERIPSPGETVLSTGFSTARGGKGQNQ
                     AVAAARAGAPTAFIAALGDDAFGSDTRAGLIDDGVDVTGVRSLDAATGTALIAVDSVG
                     ENTIIVEAGANRLLVDLQSADAAAIAASSVLAMQLEIPLETVIAAARIARAADTTVML
                     NAAPIRDLPAELLSSLDILVVNEHEASHLAGERDWRELTEQVPVVVVTLGSEGALLLR
                     RGSDEVRVAAPSVHAVDATGAGDTFCGAFAAGLAEDMELEQALRFAVTAASLSVQSYG
                     AVPSIPLRAQIDAATTR"
     gene            6451..7389
                     /locus_tag="PLW15_01260"
     CDS             6451..7389
                     /locus_tag="PLW15_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT33059.1"
                     /translation="MTRQIGVLVAIARWWHSGGSALRRLASRPAVATSAILGLVLVIA
                     LAPDAGSVHAASLFDALRAPGQAGFVAGHRGDKDGAPENTLPAFELAINSNVAFVETD
                     IQLTSDGVPVLMHDWTVDRTTDGTGPVWNMTYDQISRLDAGSWYGPQFAGTRVPTLEQ
                     LLFLLQPTGKHAILELKGAWTEDQTQTIADLLYEYGVQGRVILAGFDLVTLQALEQRA
                     PDVPRVVITHAVIGDPAVLAKACGAIAIVTSLDYIRSDPGAVARIHDEGLGVLLYTLN
                     DTDTWAEAVALGVDGLITDKPVKLDTWLARSASGAG"
     gene            complement(7408..8739)
                     /locus_tag="PLW15_01265"
     CDS             complement(7408..8739)
                     /locus_tag="PLW15_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT33060.1"
                     /translation="MTGDRSVERLREALRIPTISRLDESLVEWDEFDRFIHTLQQLYP
                     LVHASLEREIVAGHSLLFRWVGRAADHPVVLMAHYDVVAATDRGWEHPPFEAHLVGSG
                     DDAIVWGRGTLDDKGSLIALLEAVEAALSDGIVPTHDVYLAFGHDEETSGTGAAAIAA
                     LLQERGIRPLLVLDEGGAVVHGAFPGVSGATAAVGISEKGTTLVELIVNQQGGHSSTP
                     PRLSATARLARAIVRLNGRPFPARLPEPTKEMFAALAPHATGGMGWALRSARLTGPLL
                     VRALARGNDETRAMVRTTQAVTILEAGQAANALAERATAIVNLRIAVGSSVAAAVKHL
                     RRAIHDDAVVINVLSAGEPAPVSPTGGQAWGLLSATIEAEFPGAIVTPYVQNGATDSR
                     HFTGLSSAIYRFVPFEMSREERDTLHAMNERIRVSSFLKGIEFYRTLLSAL"
     gene            8790..10625
                     /locus_tag="PLW15_01270"
     CDS             8790..10625
                     /locus_tag="PLW15_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT33061.1"
                     /translation="MRRAVLSAVAALLMVLSFGALPAAAEPAAPDAPGIVLAGVDDFS
                     FESFSADYSLSTDADGRSTLTTVETFVAVFPEIDQNHGMRRAIPGDYQGAPTDVSILS
                     VTDENGAPRPFDVETDDEGFVLVTSREDGFVHGRQTYVFTYTQRNVTRYFADTNDDEF
                     FWDANGTGWSQAFGSVSARVHVPTALAGALTGQNACYRGYEGSTDTCEIVVSEEAGGT
                     VLSTAVDQIGPYENVTLAIGFAPRTFVPRDDSYLGSSQAPLQLLAVAASIAALIWSII
                     LRRTVLADGRGRPTIIAEYSPPKDLDVITASVLIKRTQRAAAAQFVDFAVGRRIRIVE
                     TETQSWFSKSTTYLLELLDARGLAGPQLALAVALFGYQLQPGTGYLMSGKDVTLSELV
                     RGIIQNATAATTREGLRKSPRVGAAILPSLIAILAAVASFVTGIAMLDNSEGGIIPFL
                     LFIPPVIVALAVFGLVFRTPLTEKGAELRDHLKGLELYIRLAEADRLKMLQSPTGAER
                     EAISTSDPRQIIDVYEKLLPYAVLFSLEKQWAQELGKYYTEQSPDWYSGTGAFNAAVF
                     ASSISSMSTTAASSYSGSSSSSSSGGSGGGGSSGGGGGGGGGGGV"
     gene            complement(10622..11005)
                     /locus_tag="PLW15_01275"
     CDS             complement(10622..11005)
                     /locus_tag="PLW15_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33062.1"
                     /translation="MTLNANYPGARLGSGRAPLEGSDRGLDALVGLVILVAEVLIGFL
                     AISSLYLFGLEHASDPNAFEGVQVGFLVALIGGAGFVALTTLTYLMRVIRGRRSWTAP
                     LWGTVLMTVSVVTGYFIMTSGITGG"
     gene            complement(11019..11657)
                     /locus_tag="PLW15_01280"
     CDS             complement(11019..11657)
                     /locus_tag="PLW15_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33063.1"
                     /translation="MGLFDSIAKGFQNLTGGVDTELIETGVLARGEILSISLSGTTVQ
                     IMNNLVERTCTFVLNVIMDGQKPFQATVSQRVQEVMIPQLQQPGVVVAVRVDAADHSR
                     VAIDFGSQPPEVNLPESTGPGSAAYILEHGKPITVVLVANQPIGVNNAKGDPVHALTL
                     TVATGVPTPYQIQVGNAIPATALPLVYPGSKLHAKLGDAPGDVVVDWAAGAA"
     gene            11759..12112
                     /locus_tag="PLW15_01285"
     CDS             11759..12112
                     /locus_tag="PLW15_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33064.1"
                     /translation="MSASYPTPARRTALIPSIIAVIALLAGVALIESDGFIVIRYIVS
                     IFALIVAVFGWQARQWWWIVPLAAIAVLWNPVFPLDLNSPDVWLGLQYGAALVFLAAG
                     ILIKVVDTGGASAKR"
     gene            12365..14431
                     /locus_tag="PLW15_01290"
     CDS             12365..14431
                     /locus_tag="PLW15_01290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT33065.1"
                     /translation="MRPGDDVGVIPVLARAAREVETAVQKGPLKPANRARFQVATLLL
                     REERARVKADKTLTDAERANEQKRLDGLAGILAKTAARDTSLIVMLGDDAPVTAAAKA
                     LRRDLLLAAGVELSPDELIITTEPKLVDKQVEKQVVPASVRQMQMSNPFLAPDFSAVA
                     AKPATVNRLANWELIEPLFRSFEYGAGGGAATMDLPEAGSLRTQGDLELMRHQARFVE
                     EVKRGHRTFLLADEPGLGKTAQALLAAAASNSYPLLVVVPNVVKTNWAREVGLWTPGR
                     TATVVHGDGNDVDAFSDVVIVNYEILDRHVGWLGRFGFKGMVVDEAHFIKNLKSERSK
                     HVLALSRSIRATYPKALLMALTGTPLINSIEDFRAIWQFLGWIDDKKPLAALMNKLEE
                     TGLTPADFGFFAEARRAVIDMGIVRRKKVDVAADIPARRIADIPVELDDDLGRSIRAA
                     EAALTARLVDRFNRVRSLKPDATDDELIRVVAHAELEESKGATTGENVFTMVRRIGQA
                     KATLAADYTSQLARSVGKVVFFAKHIDVMDQAEEHFAKLGLKSVSIRGDQSAKARQNA
                     IDSFDSDPEVSVVVASLTAAGVGLNLQAASNVVLAELSWTSAEQTQAIDRVHRIGQEL
                     PVTAWRIIAAHTIDARIAELIDSKAGLAARALDGSDVDVAAESNIQLEALSSLLRDAL
                     GERDAD"
     gene            complement(14458..15813)
                     /locus_tag="PLW15_01295"
     CDS             complement(14458..15813)
                     /locus_tag="PLW15_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT33066.1"
                     /translation="MTFPPAEPGFALPLEWLRPWMSEQRWYANRGALPVLEEIGSLRM
                     PSPGDATVIVHLLLDHTVGKPALYQVPLSYRRQALRVGWIGDLDGWQVHDAPHDPEFA
                     QALLGAILAHRVLTGDRASGAGLLAGTDAASEPTTHSRVLSGEQSNTSIIYEGSGRPI
                     IVKLFRALHHGENPDVELQTALAAAGSTAVPNPVGSIVATWSDRGRSDGRARGHLAFA
                     QEYLPGSTDGWRVALAAAEAGIDFSTEARELGIATAGVHATLAATLPTRPATADDVAI
                     AVAGMRARLERAIAEAPRLAACSPAITATFDRAEAALWPAQQRIHGDLHLGQVLAVPD
                     RGWVIVDFEGEPLRAMNERSGLDSPLRDVAGMLRSFDYIAGSIAISGASRDARDWADA
                     ARAAFNAGYGAASGRDLRSNGALVDAFELDKALYETVYEARNRPDWLPIPVGAIERLT
                     A"
     gene            15913..16842
                     /locus_tag="PLW15_01300"
     CDS             15913..16842
                     /locus_tag="PLW15_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33067.1"
                     /translation="MAVRAPALRRVPVWMWRALMAHLAPRELRRFNANPISDVSYTLD
                     VDYVGDSTREHRLDVIAPRDIPGPLPVYVYFHGGGWTSGDKSALTKYCATQALGGMIV
                     VNANYRMAPRATMQHMMQDAHAVLRWISTTIAEFGGDPDRVVLGGDSAGGQISALLAA
                     STTDAELARHYGLTPVLATVRGVVQHCSAVDFSVMFERGFILSLEFVRMLLPRRDRGL
                     PLRSAAHYLSPIEWVDSTFPPALVTTSRKDPFYQANLNFIAELMRKGVPVESHIDEFA
                     DHTWQQDSHHPGSAVVYERLQAFVRRVAPVPVG"
     gene            16878..17807
                     /locus_tag="PLW15_01305"
     CDS             16878..17807
                     /locus_tag="PLW15_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT33068.1"
                     /translation="MTLDESRAAEPGPSDDARRRARQLDETLPDIDWTVPPRGAVRGW
                     VDAPSGRIATLSQGDPAAPLVLLVPGVTGSKEDFALMLPLLADAGFHAVSYDMAGQYE
                     SRDAGPEHLRPPRPNYDHDLFVGDLLFMLESAPGPAHVLGYSFAGTVAQIAYTRSPER
                     FASLTLLSCPPEPGQGFRGVKRIGPISHFTTGRIGAALMIWGVRRNFTHVPPGRLAFV
                     RHRFGLTRLSAVADIISLMKRSPDLREALAMSSIPKLVAVGEHDLWPRELHAEFAASI
                     GAELATYPTGHSPCETAPHELTLDLLGLYARAR"
     gene            complement(17832..18587)
                     /locus_tag="PLW15_01310"
     CDS             complement(17832..18587)
                     /locus_tag="PLW15_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT33069.1"
                     /translation="MTRVSVVIPALNDSPMLANCLRALAAQTRLADEIIVVDNGSTDD
                     TAAVALAAGALVIEEPLRGIFPATAAGFDAAGGDLLARLDADSVPPADWLERIVTAFE
                     NDPHLAALSGPGDFYGSNAFVHWVAENLYIGGYIWFVGWLVGHAPLFGSNLALRKGTW
                     ARLRTTVHRSMREIHDDLDLAIHIEPDMPVVFDKTLRVGVSARPFQSISGLGRRLTWA
                     YTTLSLNSREKPFSQRRAAQRAWEREQAEEAFG"
     gene            18640..>19190
                     /locus_tag="PLW15_01315"
     CDS             18640..>19190
                     /locus_tag="PLW15_01315"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT33070.1"
                     /translation="MNLTRMRTLGAALVAAALAFAGPAISADAVAPSALSDVSDFDFE
                     SFSAEYHLDTDGSGRATMRVVETLVARFPDFDQNRGIIRAIPLKDGEYPLDVTMMSVT
                     DAQGAPVYYERNDYDGFAEFALGTDEFVHGRTTYVLEYTINNPIRHFADSGGDEFYWD
                     INGNGWQQQFDTVSAKVFLSPKLA"
BASE COUNT         3092 a         6551 c         6413 g         3134 t
ORIGIN      
        1 gcacgctcgg ggtgagctgg ggacgcacga tggatgcgat cgccggccat ctcgtggccg
       61 gatgggcgac cgcagtgacg gtggtgcaga tcaacggcgc ggtcagcctc aaccggcgcc
      121 cgacgacggc cgctaacacc gcggccacca ttgcccagaa ggccggcgga caggtgatcc
      181 tgctccccag ccccgcgatc ctcgaacgcc tcgagaccaa gcgggccatc gagtcggacc
      241 gcaccgtggc gggcgtcatc gaactcgcgg ccggtgcatc cgcctacctg ttcagcgggg
      301 gagtggccga cggaacctcc gcactcgtgg aaagcggcta tctgtccggc acggatgtca
      361 ccgagttgcg tcgcaagggg gcgatcggcg acgtcgtcgg tcgctttatc gatgccaacg
      421 ggaatatcgt tgatcccgga cttgatgagc gcaccatggg tctgagcttg gagcaactcc
      481 gagcagcagc gaatgccatc ttcgttgtct cgggcgaggc taagcaccct gtcgcgcgtg
      541 cggtcgtgac gagcggactg tgcaccgtaa tcgtgacgga cgaagcgacg gcactggcac
      601 tactggagga gaacgcatga caccgagtgc agcgctggcc ccggccgagc gggccctggg
      661 gctcatcggc cccgacctca ccgagaagag tcttcgcctc cacctggagg gcctctcggg
      721 aatcgacgcc gtcggtctcg aggagcgcgc ggccgcccta ggtacccgct cgatcaagac
      781 cacctcgaag gcatgggcgc tcgacaccat catcaggctg atcgacctca cgaccctcga
      841 gggcgctgac accgacggca aggtgcgctc cctggtcgcc aaggcgttga atcccgacgc
      901 gtcagacccc accgttcccc gcgttgccgc ggtgtgcgtc tacggtgaca tggtccccac
      961 cgccgtggat gcgctcggct cggcgcacgg tgaccccgac gatggcaaca tcagcgtcgc
     1021 cgccgtggcc accgccttcc cgagcggtcg agcctcgctc gccgtgaaga cactggacac
     1081 cgccgaggcg gtcgccgcgg gagcggacga gatcgacatg gtgatcgatc gcggtgcctt
     1141 cctgtcgggc aactacgggc tcgtcttcga ccagatcgtg gcagtcaagg aggcctgtcg
     1201 tcgtgaagat ggcagctatg cccacctcaa ggtgatcctc gagacgggtg agctgaacac
     1261 ctacgacaac gtgcgccggg catcctggct cgcgattctt gcgggtggcg acttcatcaa
     1321 gacctccacg ggcaaggttg ctcccgccgc cacgcttccg gtgaccctgc tgatgctcga
     1381 ggtggtgcgc gactggcacc ggctgacggg cgagcgcatc ggcgtgaagc cggccggggg
     1441 catccgtacc tcgaaggacg cgatcaagta cctcgtgacc gtcgccgaga ccgtgggaga
     1501 ggagtggctt cagccgcacc tgttccggtt cggggcatcg agcctgctca acgacgttct
     1561 gctccagcgc cagaagctca ccaccggccg ctactcgggc cccgactacg tgacgattga
     1621 ctgagaacaa tgactgagat cgtgaagccg caccgttttc tcgactacgc gcccgcgccc
     1681 gagtcgacga gcatcctgca cctgaagaag agctacgggc tcttcatcga cggggagttc
     1741 gtcaagggtc acggcaagcc gttcctcacc gtcgctccgg caaccgagga gaagatcgcg
     1801 gagatcgcca acgccaactc caaggatgtg gatgccgcgg tggccgccgc gcgtcgagcc
     1861 tacgatcgca cctggtcgaa gctctcgggc gccgaccgcg gcaagtacct gttccgcatc
     1921 gcgcggctcg tgcaggagcg cgcccgagaa ctggccgtcg cggagagtat cgacaacggc
     1981 aagccgatca aggagtcccg cgacgtcgac gtgcccctgg tcgcggcgtg gttcttctac
     2041 tacgcgggct gggccgacaa gctcgactac gcgggcctgg gcccgaaccc tcgtgcactg
     2101 ggggtcgccg cccaggtcat tccgtggaac ttcccgctgc tcatgctggc ctggaagatc
     2161 gcaccagcgc tcgccgccgg caacacggtg gtgatcaagc ccgcggagac gacgccgctg
     2221 agttcgctca tcctggccga gatccttcag caggccgaac tgccggcggg tgtcgtcaac
     2281 atcatcaccg gagcgggaga cacgggccag gcgctcgtca accacccgga tgtcaacaag
     2341 gtcgccttca ccgggtcgac cgccgtcggg cgcgcgatcg ccaagtcggt cgccggttcc
     2401 ggtaagaagc tcacgctcga gctgggtggc aaggccgcca acatcgtctt cgacgacgcg
     2461 ccgatcgatc aggccgtcga ggggatcgtc aacgggatct tcttcaacca gggtcacgtc
     2521 tgctgcgccg gttcgcgact gctcgtgcag gagaacgtgc acgacgaggt cgtcgaccgg
     2581 ctcaagctgc gcctctcgac gctgcgcctc ggcgacccgc tcgacaagaa caccgacatc
     2641 ggagcgatca actcgcgcga gcagctcgag cgcattcgtg agttgagcga catcggcgag
     2701 cgcgagggtg ccgagcgctg gacggcgccc tgcgtcatcc cggacaaggg cttctggttc
     2761 gccccgacca tcttcaccaa tgtgtcgacg agttcgacga tcgcccgcga ggagatcttc
     2821 ggcccggtgc tgagcgtgct gacgttccgc acccccgccg aggcgatcgc caaggccaac
     2881 aacaccccgt acggcctgtc ggccggcatc tggagcgaga agggctcgcg catcctcgcg
     2941 gtcgccgacc agcttcgtgc cggtgtgatc tgggcgaaca cgttcaaccg cttcgacccg
     3001 gccagcccct tcggcggcta caaggagagc ggctacggcc gggaaggcgg gcgtcacggc
     3061 ctcgccgcgt atctgcaggg agcatcatgg taggccgtct cgcggtaccc aagacctaca
     3121 agctgtacat cggcggaaag ttcccgcgca gcgagagtgg gcgcacctac gaggtgctct
     3181 cgaagaaggg tgccttcctt gccaacgccg ccctcgccag ccgcaaggat gcccgtgacg
     3241 cggtagtcgc ggcgcgtgcc gccttcggcg gctggtcgtc cgccacggcg tacaaccggg
     3301 ggcaggtgct gtatcgcatc gccgagctgc tcgagggccg tcgcgaccag ttcgtggacg
     3361 agttggtctc ggcggaggga ctgtcgcgca cggccgccgt caagcaggtc gacacggcga
     3421 tcgacgcctg ggtctggtac gcggggtggg ccgacaagta cgtgcaggtt gccggtaacg
     3481 gcaacgcggt gtcgggtccg tacttcaacc tctcgacgcc cgagccgacc ggtgtcgtgg
     3541 cgatcgtcgc tccgcagtcg ggcgggtcgc tgctcggtct cgtcagcgtg gtcgccccgg
     3601 cgatcgtctc gggcaacacc gtcgtcgtgg tggctcagca gtcagcaccg ctcagcgcga
     3661 tcagtctcag tgaggttctc gcgacgagcg atgtgcccgg gggagtggtg aacatcctca
     3721 ccggttctcc cgcggagatc gcaccgtggc tcgccggtca cgccgacgtg aacgcgatcg
     3781 acctcgccgg agcaggtgac ctcgagtggg ttgacatgca gatcactgcc gccgacaccc
     3841 tcaagcgagt catcgcgccg gaggtcggcg cgcccgcgcc gtcgcttcac cgcatcgtcg
     3901 ccctgaccga gacgaagacg gtctggcaca cgaagtcgct gatctgagcg tgccgacctc
     3961 gggtcgctgg tgtgaggtag cggggcgggc aaccgccagt cggcacacag gtcgacgcac
     4021 gtaggctcga cccatgcgca tcctcgtcac cggcgcgacc gggtacatcg gagggcgcct
     4081 cgcgccgcgg ctggtcgacg ccgggcacga ggtgaggatt ctcgtgcgag acgctgcccg
     4141 cgttcgcgac gtgccgtggg ccaagcgggt cgaggtggta accggcgacc tcagcgatgc
     4201 cgcgacgctg ccggctgctt tcgagggaat cgatgtcgcc tactacctcg tgcacgccat
     4261 gggggccggg ggagatttcg agcgcacgga actgacctcg gcgcgcaacg ttgctgcggc
     4321 agcgaaggcc gccggtgtga agcgcatcgt ctacctcggg ggtcttcacc ccgacgttcc
     4381 cctcgcacag ttgagcactc accttcgcag tcgcgccgag gtgggggaga ttctgatcgc
     4441 gtcgggcgtt ccgactgcgg cgctccaggc gggcaccgtg atcggctcgg gttccgcgtc
     4501 gttcgagatg atccggcacc tcaccgaggt gctgccgtac atgccagcgc cgaagtgggt
     4561 gcgcaacttc atccagccga tcgccgtccg tgacgtgctc tactacctcg tcgctgcggc
     4621 gtcgctgccc gccgaggtca accgtacgtt cgacatcggc ggccccgacg tgctgcgcta
     4681 cggccagatg atgaacgggt acgccctcga ggcgggcctg cgccagcgtc cgatcgcctc
     4741 gctgccggtg ctgacgccct ggctcgcgtc gcagtgggtc aacctcgtca caccgattcc
     4801 gcgccagctc gccgtgccga tcatcgcgtc gctgcaatac gactgcgtgg cgagcgagca
     4861 cgacatcgcc cactacattc ccgaccccga gggcgggctc acgggctacc gcaaggcggt
     4921 gcgcctcgca ctcgccaagg tgcgtgacgg tcaggtggag acgagctggc agaacgccgc
     4981 gatcgccggc gcccccagta acgcgctgcc gagtgacccc gactggtcgg gccacacggt
     5041 gtacacggac gtgcgggagc agcactccgc ggcatccgtc gaccagctct gggaggtcgt
     5101 cgagtcgatc ggcggcgaga acggctggta ctcgtttcct cttgcctggg cggtgcgcgg
     5161 ctggctcgac aagctcgtgg gcggtgtcgg cctgcgtcgc ggccgacggg atgcccggcg
     5221 tctcgaaccg ggggatgccc tcgacttctg gcgggtcgag gacatcgacc gcgggcactt
     5281 ccttcgcctc cgtgcggaga tgaaggtgcc cgggcgagcg tggctcgaga tgacgaccga
     5341 ggccgatccc cagggcggct cgacctatcg gcagcgggcc gtgttcttcc cgtcggggct
     5401 cggcggacga ctctactggt actcgatact tcccttccac ggggtgatct tcagcggcat
     5461 ggccaaccag atcacgaaga gcgccgagcg gccggtacgg tagagcagtg tcatccgtca
     5521 tcgtcgtcgg cagcgccaat gtcgaccagg tcttccgagt tgagcgcatc ccctctccgg
     5581 gcgagaccgt cttgtccacg ggattctcca cggcacgcgg gggcaagggg cagaaccagg
     5641 ccgtcgccgc cgcgcgcgcc ggcgcaccca cggcattcat cgcagccctc ggtgacgatg
     5701 ccttcggctc cgacactcgg gcgggcctga tcgacgacgg cgtcgacgtg acgggtgtca
     5761 ggtccttgga cgccgcgacc ggcaccgcac tcatcgctgt cgattcggtg ggtgagaaca
     5821 cgatcatcgt cgaggccggc gccaaccgac tcctcgtcga tctgcagtcg gcggatgccg
     5881 cggcgatcgc cgcatcgtcg gtgctcgcca tgcagctcga gatccccctg gagaccgtca
     5941 tcgcagcggc ccggatcgct cgggccgccg acaccacggt gatgctcaac gccgcaccca
     6001 tccgcgacct gcctgccgag cttctgagtt cactcgacat cctcgtcgtc aacgagcacg
     6061 aggcgagcca cctcgccggg gagcgcgact ggcgggagct gaccgaacag gttcctgtcg
     6121 tggtcgtgac cctcggctcc gagggcgcgc tgttgctgag gcgaggctcg gacgaggtgc
     6181 gggtggcggc gccatccgtt cacgcggtcg acgcgaccgg cgccggcgac accttctgcg
     6241 gggccttcgc ggcggggctg gcggaggaca tggagttgga gcaggctctg cggttcgcgg
     6301 tgaccgccgc ttcgctctcg gtgcagagct acggggcggt tccctcgatt ccgttgcgag
     6361 cgcagatcga cgccgcaact acacgctgac ccatatgtaa cagatcggta acgaagacgc
     6421 tatagtcgat tcacgctcgc gacggtgcga gtgacccgac agattggcgt ccttgtggcg
     6481 attgcgcggt ggtggcactc cggtggcagt gcgctgcgca ggcttgcctc ccgccccgcc
     6541 gtcgcgacct cggcgattct cgggctcgtc ctcgtcatcg ccctggcacc cgacgccggc
     6601 agcgtgcatg ccgcgagtct gttcgatgca ctgcgagctc cgggacaggc ggggttcgtg
     6661 gccggccatc gcggtgacaa ggatggcgct ccggagaaca ctctccccgc attcgagctc
     6721 gcgatcaaca gcaacgtggc gttcgtcgag acggacatcc agctcacctc cgacggcgtg
     6781 ccggtactga tgcacgactg gaccgtcgac cggacgaccg acggcaccgg cccggtctgg
     6841 aacatgacct acgaccagat cagtcgactg gatgccggca gctggtacgg tccccagttc
     6901 gccggtaccc gagtgcccac cctcgagcag ctcctgttcc tactgcagcc caccggcaag
     6961 cacgcgatcc tcgaactcaa gggggcgtgg accgaggatc agactcagac cattgccgac
     7021 ctgctgtatg agtacggcgt gcagggccgg gtcatcctcg ccggcttcga cctggtcaca
     7081 ctgcaggcac tcgagcagcg cgcacctgac gttccccggg tggtgatcac tcatgcggtg
     7141 atcggcgatc ctgcggtgct cgcgaaggca tgcggcgcga tcgcgatcgt caccagcctc
     7201 gactacatcc gcagcgatcc tggggccgtg gcgcgcatcc acgatgaggg tctgggcgtg
     7261 ctgctgtaca ccctcaatga caccgacacc tgggccgagg ctgtcgcgct tggggtcgac
     7321 gggctcatca ccgacaagcc cgtcaagctc gacacctggt tggccagaag cgcttccggc
     7381 gcaggctagg cgccgcccac gaccgggcta cagcgcgctg agcagggtgc ggtagaactc
     7441 gatgcccttg aggaacgagc tcacgcggat ccgttcgttc atcgcgtgca gggtgtctcg
     7501 ctcctcgcgt gacatctcga acggcacgaa ccggtagatg gcgctcgaga ggccggtgaa
     7561 atgccggctg tcggtcgcgc cattctgcac gtacggggtg acgatcgcgc cggggaactc
     7621 cgcctcgatc gtcgccgaga gcagtcccca ggcctggcct cccgtcggcg agaccggcgc
     7681 cggctcgcca gcgctgagaa cattgatcac cacggcatcg tcgtggatgg cgcggcgcag
     7741 gtgcttcacc gccgcggcaa ccgacgatcc gaccgcgatt cgcaggttga ctatcgcggt
     7801 tgcgcgctcg gccagggcat tggcagcctg accggcttcc aggatggtca cagcctgggt
     7861 ggtgcgcacc atggcgcggg tctcatcgtt gccccgtgcc aacgcccgca ccagcagcgg
     7921 accggtgagc cgtgctgatc gcagcgccca tcccatgccc cccgtcgcgt gcggcgcgag
     7981 cgccgcgaac atctccttgg tgggctcggg aagtcgggcg ggaaacgggc gtccgttcaa
     8041 ccgcacgatc gctcgcgcca agcgtgcggt cgcgctgagc cgcggcggag tggaggagtg
     8101 tccgccctgc tggttcacga tcagctcgac gagcgtggtg cccttctcgc tgatacccac
     8161 cgccgcggtc gcaccactca cgccgggaaa ggcgccgtgc acgaccgcgc cgccctcgtc
     8221 gagcacgagc agggggcgga tgccacgctc ctggagcagt gcggcaattg cggccgcccc
     8281 ggtaccgctc gtctcctcgt cgtgcccgaa ggcgagatat acatcgtgcg tgggcacgat
     8341 gccgtccgac agtgcggcct ccacggcttc gagcagagcg atgagcgagc ccttgtcgtc
     8401 gagcgtgccc cggccccaca cgatcgcgtc atcaccggag ccgacgaggt gcgcctcgaa
     8461 tggcgggtgc tcccagcctc gatcggttgc tgcgacgacg tcgtagtggg ccatgagaac
     8521 gaccggatgg tcggcggccc ggcccaccca tcggaagagc agggagtgcc cggccacgat
     8581 ctcgcgctcg agcgaggcgt gcaccagggg atagagctgc tgcagggtgt ggatgaaccg
     8641 atcgaactca tcccactcga cgagcgactc gtcgagccgt gagatggtcg gtattcgaag
     8701 cgcctcccgg agcctctcga ctgaacggtc accggtcatg ggtcgactct acctagctcg
     8761 gggccgcgcg catcccgtag tttgggcaca tgagacgtgc cgtgctgtcc gctgtcgccg
     8821 ccctgctcat ggtgttgtcg ttcggcgccc tccctgcggc ggccgagcct gctgcgcctg
     8881 atgcaccggg aatcgtgctc gccggggtcg atgacttctc cttcgagagc ttcagcgccg
     8941 actactcgct gtcgaccgat gccgacggtc ggtcgactct cacgacggtc gagaccttcg
     9001 tcgccgtctt ccccgagatc gatcagaacc acggcatgcg tcgggcgatc ccgggcgact
     9061 accagggtgc gccgacggat gtctcgatcc tgtctgtcac cgacgagaac ggtgcaccgc
     9121 ggccattcga cgtggagacc gacgacgagg gcttcgtgct cgtcaccagc cgtgaagacg
     9181 gattcgtgca cggccgccag acctatgtgt tcacctacac acagcgcaat gtcacccgct
     9241 acttcgccga caccaatgac gacgagttct tctgggacgc caacggcacg ggctggtcgc
     9301 aggccttcgg ttcggtctcg gcgagggtgc atgtgcccac ggcgctcgcc ggcgcgctga
     9361 ccggccagaa cgcctgttac cgcggctacg agggctcaac tgacacctgc gagatcgtcg
     9421 tctccgaaga ggccggaggc acggtgttga gcaccgctgt cgatcagatc ggaccctacg
     9481 agaatgtcac gctcgcgatc ggtttcgccc cccgcacctt cgtgccccgg gatgactcgt
     9541 acctcggttc gtcgcaggcg cccctccagc tgctcgcggt ggccgccagc atcgcagcgc
     9601 tgatctggtc gatcatcctt cgccgcaccg tgctcgcgga tgggcgcggg cgcccgacga
     9661 tcatcgcgga gtactcgccg ccgaaggatc tggacgtgat caccgcatcg gtgctgatca
     9721 agcgcaccca gcgcgctgcg gcagcgcagt tcgtcgattt cgccgtggga cgccgcattc
     9781 gcatcgtcga gaccgagacc cagagctggt tctcgaagag cacgacctac cttctcgaac
     9841 tgctggatgc ccgcggtctg gccggaccgc agctcgccct cgcggtcgca ctgttcggct
     9901 accagctgca gccgggaacc ggctacctga tgtccggcaa ggacgtcacc ctcagtgagc
     9961 tcgtgcgggg gatcatccag aatgccaccg cggcgacgac ccgggagggg cttcgcaaga
    10021 gtccgcgggt cggggcggcg atacttccga gcctcatcgc gatcctggcc gcggtcgcat
    10081 cgttcgtcac gggtatcgcg atgctcgaca actcggaggg cggcatcatc ccgttcctgc
    10141 tcttcattcc acccgtgatc gtcgcgctcg ctgtgttcgg gctggtgttc cgcaccccgc
    10201 tcaccgagaa gggggccgag ctgcgggatc acctgaaggg cctcgagctc tacatccgcc
    10261 tcgcggaggc cgatcgcctg aagatgctcc agagccctac gggcgccgag cgggaggcga
    10321 tctccacgag cgatccgcgc cagatcatcg acgtctacga gaagcttctg ccgtatgccg
    10381 ttctcttcag cctggagaag cagtgggctc aggagctcgg caagtactac accgagcaat
    10441 cgcccgactg gtactcgggt acgggcgcgt tcaatgccgc ggtattcgca tcgagcatca
    10501 gttcgatgtc gacgacagcg gcgagctcgt actcgggcag ttcgtcgagc agttcgagcg
    10561 gcggctccgg cgggggtggg tcatccggtg gtggcggggg tggcggcggt ggcggtggcg
    10621 tctagccgcc cgtgatgccg ctcgtcatga tgaagtagcc ggtcacgacc gacaccgtca
    10681 tgagcacggt gccccacagc ggcgccgtcc agctgcgacg gccccggatc acgcgcatca
    10741 ggtacgtcaa cgtcgtcagc gcaacgaacc cggcgcctcc gatgagcgcg acgaggaagc
    10801 ccacctgcac cccctcgaac gcgttgggat ccgacgcgtg ctcgagcccg aacaggtaga
    10861 gcgacgagat cgcgaggaat ccgatgagca cctcagcgac caggatgacc agccccacga
    10921 gtgcgtcgag cccccgatcg ctgccctcga gcggcgcgcg acccgagccg agacgggcgc
    10981 cggggtagtt cgcgttgagg gtcatgccgt cagcctatct acgccgcacc ggccgcccag
    11041 tcgaccacga cgtcacccgg cgcgtccccg agtttcgcgt gaagtttcga ccccgggtac
    11101 accagcggca gcgcggtggc gggaatcgcg ttgcccacct ggatctggta cggcgttggc
    11161 acgcccgtgg ccacggtcag agtcagtgca tgcacggggt cgcccttcgc gttgttcacc
    11221 ccgatgggtt gattggccac gagcacaacg gtgatcggct tgccgtgttc gaggatgtag
    11281 gcggcgctgc ccggtcccgt tgactccggc aggttcacct cgggcggctg gctgccgaag
    11341 tcgatggcta ccctgctgtg gtctgcggca tccacgcgca cagcgacaac gacacccggt
    11401 tgctggagct gggggatcat gacctcctgc acgcgctgcg agactgtggc ctggaacggc
    11461 ttctgaccgt ccatgatcac gttgagcacg aaggtgcagg tgcgttcgac gaggttgttc
    11521 atgatctgca ccgtcgtgcc cgagagcgag atggagagga tctcgccgcg cgcgaggaca
    11581 cccgtctcga tcagctcggt gtccacccct ccggtcagat tctggaaccc cttggcgatg
    11641 ctgtcgaaca atcccaccgt cgtgcccttc gtcgagtcga gtgctgctat cgacgcacag
    11701 actagcgccg gggcagcgcc cgcgataggg cgccgccgac cgggataggc tcctgagggt
    11761 gagcgcttcc tatcccacgc ccgcccgccg caccgccctc atcccgtcga tcatcgcggt
    11821 gatcgcgctg ctcgccgggg tcgctctcat cgagagcgat gggttcattg tgatccgcta
    11881 catcgtgagc atcttcgccc tgatcgtcgc ggtgttcggt tggcaggcgc gccagtggtg
    11941 gtggatcgtg ccgctggcgg cgatcgccgt gctgtggaac ccggtcttcc cactcgacct
    12001 gaactcgccg gatgtctggc tcggcctgca gtacggtgcc gccctggtgt tccttgctgc
    12061 gggcatcctc atcaaggttg tcgacacggg cggcgcttcg gccaagcggt gacggggcaa
    12121 acgggggtac actgatccca acgcccgctg agcccaccag tcgcctcgcg actgggtctg
    12181 gatgtctcaa gccgggcgcc gtcatccggt agttgtgccg atggccacac gcatacgaag
    12241 tgccgggcgg aagcgccatg cactaggaga acccttggta tcttccggac agaccgagcc
    12301 gggccagcgc cagaagcgcc agcgatccgc gtcgcagcgc cagcagtcca cccagcgtcg
    12361 ccgcgtgcga ccgggcgacg acgtcggtgt catccccgtg ctcgcacgcg ccgcccgcga
    12421 ggtcgagact gccgtgcaga aggggccgct caagccggcc aaccgcgcgc ggttccaggt
    12481 tgccaccctc ctgctgcgcg aggagcgcgc gagggtcaag gccgacaaga cgctcaccga
    12541 cgcggagcgc gccaacgagc agaagcgtct cgatgggctt gcgggcattc tcgcgaagac
    12601 ggccgcgcgc gatacgagcc tcatcgtcat gctcggcgac gacgcccccg tcactgccgc
    12661 cgcgaaggcg cttcgccgcg accttctcct cgcggccgga gtcgagctga gccccgacga
    12721 gctgatcatc acgacagagc ccaagctcgt cgacaagcag gtggagaagc aggtcgtgcc
    12781 cgcgtcggtg cgtcagatgc agatgtccaa cccgttcctc gcgcccgact tcagcgccgt
    12841 cgcggccaag cccgcgacgg tcaaccggct cgccaactgg gagctcatcg agcccctgtt
    12901 ccgctcattc gagtacggtg ccggcggggg agccgccacg atggatcttc ccgaggccgg
    12961 ttccctgcgc acgcagggcg acctcgagct catgcgccac caggcgcgtt tcgtcgagga
    13021 ggtcaagcgc ggccaccgca cgttcctgct cgccgacgaa ccgggactcg gcaagacggc
    13081 ccaggcgctg ctcgccgctg ccgcatccaa ctcctatccg ctgttggtgg tggttcccaa
    13141 cgtggtgaag accaactggg cgcgcgaggt cggactctgg acccccggtc gcacggccac
    13201 ggtcgtgcac ggcgacggca atgacgtcga cgccttcagc gacgtggtga tcgtcaacta
    13261 cgagatcctc gaccgccacg tcggttggct cggtcggttc ggcttcaagg ggatggtcgt
    13321 cgacgaggcg cacttcatca agaacctgaa gtccgagcgc tccaagcacg tgctcgcgct
    13381 gtcgcgcagc atccgggcca cctacccgaa ggccctcctg atggcgctca cgggtacgcc
    13441 gctgatcaac tcgatcgagg acttccgggc gatctggcag ttcctcggct ggatcgatga
    13501 caagaagccg ctggccgccc tcatgaacaa gctcgaggag accgggctca cccccgccga
    13561 cttcggcttc ttcgcggagg cgcggcgtgc cgtgatcgac atgggcatcg tgcgacgcaa
    13621 gaaggtggat gtcgccgccg acatccccgc ccgccgcatc gccgacatcc ccgtggagct
    13681 cgacgacgat ctcggtcgat cgatccgtgc cgccgaggca gcactgacgg cgcggctggt
    13741 cgaccgtttc aaccgggtgc gctccctcaa gcccgatgcc accgacgacg agctcatccg
    13801 cgtggtcgcc cacgccgagc tcgaggagtc gaagggcgcc acgaccggcg agaacgtctt
    13861 cacgatggtt cgcaggatcg gacaggcgaa agccaccctc gcagcggact acacctcgca
    13921 gctcgcgcga tccgtgggca aggtcgtgtt cttcgccaag cacatcgacg tgatggacca
    13981 ggccgaggag cacttcgcga agctgggtct gaagtcggtg tcgatccgcg gcgaccagag
    14041 cgcgaaggcg cgtcagaatg cgatcgactc cttcgactcc gaccccgagg tctccgtggt
    14101 cgtggcctcg ctcacggccg ccggtgtcgg gctcaacctg caggcggcgt ccaacgtcgt
    14161 gctcgccgag ctgagctgga cgagcgccga gcagacccag gccatcgacc gcgtgcaccg
    14221 catcggtcag gagctgccgg tcacggcgtg gcgcatcatc gccgcgcaca cgattgacgc
    14281 ccgcatcgcc gaactcatcg acagcaaggc gggacttgcc gcacgcgccc tcgacggatc
    14341 cgacgtggat gtcgcggccg agtccaacat ccagctcgag gcgctgtcga gcctgctgcg
    14401 cgacgcgctc ggggagcgcg acgccgacta gttccgcgct ctgaacggcg tgaccaacta
    14461 ggcggtcagg cgctcgatcg cccccaccgg gatcggaagc cagtccggtc tgttgcgcgc
    14521 ttcgtagacg gtctcataga gagccttgtc gagctcgaac gcgtcgacga gggcaccatt
    14581 cgaccggagg tcgcggcccg acgccgcgcc gtaccccgcg ttgaaggctg ctcgcgccgc
    14641 gtcggcccag tcgcgtgcat cgcggctcgc cccggagatc gcaatcgatc cggcaatgta
    14701 gtcgaacgac cggagcatgc cagcgacgtc acgaagcggg ctgtcgagac cggagcgttc
    14761 gttcatcgcc cgcagcggtt caccctcgaa gtcgacgatg acccagcccc gatcgggcac
    14821 ggcgagtacc tgcccgaggt ggaggtcgcc gtggatgcgc tgctgggcgg gccacagcgc
    14881 cgcctcggcc cggtcgaacg tggcggtgat cgcgggggag caggcggcga gccgaggtgc
    14941 ctcggcgatg gcgcgctcga ggcgggcgcg catccccgcg accgcgatcg caacgtcgtc
    15001 agcggtcgcc ggacgagtgg gcagggtggc ggcaagcgtc gcgtgcacgc ccgcggtcgc
    15061 gattccgagt tcgcgcgcct cggtgctgaa gtcgatgccg gcttcggccg ccgccagggc
    15121 gacgcgccag ccatcggtcg agcccgggag gtactcctgc gcgaacgcga gatgcccccg
    15181 cgcccgacca tcggagcgcc cgcgatccga ccaggtggcc acgatgctgc cgaccgggtt
    15241 cggcacggcg gtggagccag ccgccgcgag cgcggtctga agttcgacgt ccggattctc
    15301 accgtggtgc agcgcccgga acagcttgac gatgatcggt cgccccgacc cctcgtagat
    15361 gatcgaggtg ttggactgct cgcccgagag cacccggctg tgcgtcgtgg gttcggacgc
    15421 ggcatccgtg cccgccagca gaccggcacc ggatgcccga tctccggtca gaacacggtg
    15481 cgcgagtatg gcccccagca acgcctgggc gaactccgga tcgtgcggcg cgtcatgcac
    15541 ctgccagccg tccagatcgc cgatccagcc cacgcgaaga gcctgccgcc ggtacgacag
    15601 gggcacttga tagagcgcgg gcttgcccac tgtgtggtcg agaagaaggt ggactatgac
    15661 cgtcgcatcg ccggggctcg gcattcgcag actgccgatc tcctcaagca caggaagcgc
    15721 gccccggttc gcataccagc gctgttcgct catccagggg cgcagccact cgagtggcag
    15781 tgcgaatccc ggctccgccg gcgggaaggt catcgctcag actaggcacg gtgcacgggg
    15841 tgaggtcaag cgcttgcaca atgtgcagtt gatgcccagc agtatccaag gcacgggtcg
    15901 tatcgtgggc acatggcagt acgcgccccg gcattgcgcc gggttccggt gtggatgtgg
    15961 cgcgcgctca tggcccatct ggcaccgcgg gagctgcgtc gattcaacgc gaatcccatc
    16021 agcgacgtga gctacaccct cgacgtggac tacgtgggtg acagcacccg cgagcatcgc
    16081 ctcgacgtga tcgctccccg cgacatcccg ggcccgctgc cggtctacgt ctacttccac
    16141 ggcgggggct ggacgagcgg tgacaagtcc gccctcacga agtactgcgc cacccaggct
    16201 ctcgggggca tgatcgtggt caacgccaac taccggatgg ccccgcgagc caccatgcag
    16261 cacatgatgc aggatgccca cgccgtgctg cgctggatct caaccaccat cgccgaattc
    16321 ggcggcgacc ccgaccgggt ggtactcggc ggcgactcag cgggtggtca gatctcggcg
    16381 cttctcgccg catccacgac cgatgcggag ctcgcccgtc actacggact gacaccagtg
    16441 ctcgcgacag tccgtggggt cgttcagcac tgcagcgccg tcgatttctc ggtgatgttc
    16501 gagcgcgggt tcatcctcag cctcgaattc gtgcgcatgc ttctgccgcg acgtgaccgg
    16561 gggctgcccc tgcgcagcgc cgcgcactac ctctcgccga tcgagtgggt cgacagcact
    16621 ttcccaccgg cgctggtgac gacctcgcgc aaagacccgt tctaccaggc gaatctcaac
    16681 ttcatcgcag aactgatgcg caagggagtc cctgtggagt cccacatcga cgagtttgcc
    16741 gatcacacct ggcagcagga ctcccatcac ccgggttccg ccgttgtcta cgagcgactg
    16801 caggccttcg tgcgtcgagt ggctccggtc cccgtcggct gattccgaac ggggcgctgg
    16861 ggcgctaacg tggctgcgtg actctggatg aatcccgagc ggcggagccc gggccttccg
    16921 atgacgcacg gcggcgcgca cgtcagctcg acgagaccct gcccgacatc gactggaccg
    16981 ttccgccccg cggtgctgta cggggctggg ttgacgcgcc cagcgggcga atcgccacgc
    17041 tctcgcaggg tgaccctgcg gcgccgctcg tgctcttggt gcccggtgtg accggttcca
    17101 aagaggactt cgccctcatg ctgccgcttc tggccgacgc gggcttccat gcggtgagct
    17161 atgacatggc cggccagtac gagtcccggg atgccggccc cgagcacctg cgtccgccgc
    17221 gccccaacta cgaccacgac ctgttcgtgg gtgacctgtt gttcatgctc gagtccgctc
    17281 ccgggcctgc gcacgtgctc gggtactcgt tcgcgggcac ggtggcgcag atcgcctaca
    17341 cccgcagccc cgagcggttc gcgagcctga cgctgctgag ttgtccgccg gagcccggcc
    17401 aggggttccg gggcgtcaag cgcatcggtc ccatctcgca cttcacgacg ggtcggatag
    17461 gtgcggctct catgatctgg ggtgttcgcc gcaacttcac ccacgtgccg ccgggacgcc
    17521 tcgccttcgt gcggcatcgc ttcggtctga ccaggctcag cgccgttgcc gacatcatct
    17581 cgctcatgaa gcgctcgccc gatctcaggg aggctctggc gatgtcgagc atcccgaagt
    17641 tggtcgccgt cggggagcac gacctgtggc ctcgcgagtt gcacgccgag ttcgcggcct
    17701 ccatcggtgc tgagttggcg acgtacccga ccggtcacag cccgtgtgag acggctccgc
    17761 acgagctcac tctcgacctc ttgggtctct acgcgcgagc gcggtgaggg cgcagccgcc
    17821 cgatcagccc tttagccgaa cgcctcttcg gcctgttcgc gctcccacgc gcgttgggcg
    17881 gcacggcgct gcgagaacgg cttctcgcgc gagttgagac tcagagtggt gtaggcccag
    17941 gtcaggcgtc gccccagacc ggagatcgac tggaaggggc gcgcggagac gccgacccgc
    18001 agcgtcttgt cgaagaccac gggcatgtcc ggctcgatgt ggatcgccag gtccaggtcg
    18061 tcgtgaatct cacgcatcga ccgatgcacc gtcgtgcgca gtcgcgccca ggtgcccttg
    18121 cgcagggcga gattggagcc gaacagcggc gcgtgaccga cgagccagcc cacgaaccag
    18181 atgtagccgc cgatgtacag gttctcggcg acccagtgca cgaacgcgtt cgacccgtag
    18241 aagtcgccgg gaccgctcag ggcagcaagg tgggggtcgt tctcgaacgc ggtgacgatc
    18301 cgctccagcc agtccgcggg gggaaccgag tcagcgtcga gccgcgcgag cagatcgccg
    18361 cctgctgcgt cgaatccggc ggcggtcgca gggaagatgc cgcgcagcgg ttcctcgatc
    18421 acgagcgcgc cggcggcgag agctacggcg gccgtgtcat ccgtgctgcc gttgtcgaca
    18481 acgatgatct cgtcggccag cctcgtttgg gctgcgaggg cgcgcaggca attcgccaac
    18541 atcggcgagt cgttcagtgc ggggatcaca acgctaacgc gagtcatttg ctcccagcat
    18601 agctacgacg cggctcgggc ccgactgtag ggtggcttcg tgaatctgac tcggatgcgt
    18661 actctgggcg ccgcgctggt ggctgcggca ctggcattcg cgggaccggc gatctcggcc
    18721 gatgcagtcg cgccctcggc gctcagcgat gtcagcgact tcgacttcga gtccttctcg
    18781 gctgagtatc atctcgatac cgacggctcg ggacgcgcga cgatgcgggt cgtggagacg
    18841 ctcgtcgcgc ggttcccgga cttcgatcag aaccggggga tcatccgcgc catccccctc
    18901 aaggacggcg agtacccgct cgacgtgacg atgatgtcgg tgaccgatgc gcagggtgct
    18961 cccgtgtact acgagcgcaa cgactacgac ggattcgccg agttcgcgct gggcaccgac
    19021 gagttcgtgc acgggcgcac aacctacgtg ctcgagtaca cgatcaacaa cccgattcgg
    19081 cacttcgccg actccggcgg tgacgagttc tactgggaca tcaacggcaa cggctggcag
    19141 cagcagttcg acaccgtatc ggcgaaggtc ttcctctccc cgaagctggc
//
LOCUS       DAOSOU010000012        18871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_2937_length_18871_cov_6.651095, whole genome shotgun sequence.
ACCESSION   DAOSOU010000012 DAOSOU010000000
VERSION     DAOSOU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 18871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18871
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2937_length_18871_cov_6.651095"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PLW15_01320"
     CDS             complement(<1..1445)
                     /locus_tag="PLW15_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33071.1"
                     /translation="MTTANDTSRRSPFSRTPDTGPRAKLSQLWPYLAEHRGILSVVVA
                     LSILAAAASLAQPLLVSQVITVVQANKPLGMLVWALVGLVVISGVLSGFQHYLLQRTG
                     TSVVLSARRQLVRRMLRLPISEFDTRRTGDLVSRVGSDTTLLYAVITQGLVDAIGGAL
                     VFIGALVAMLIIDPLLLGLTLLVVAIAVVTVVSLSGRIRVASRKQQEKVGDLAASVER
                     SLSAVRTVRASNATDREIAAVEKDAEGAWRMGIQVAKISALVVPIAGIALQVALLVVL
                     GVGGFRVASGAITVASLVSFILFLFMMIMPLGQAFGAINSVNQALGALGRIQEIISLP
                     SETDGDAAIVPAAAIETDAAITFENVEFSYPEGTVVEQVEKEVVELVETEHLEGQIST
                     SAIADSDRTVLRGVSFAAERGKRTALVGPSGAGKSTILALIERFYDPNAGVVRLGGID
                     IRGLDRTDLRAQIGYVEQDAPVLAGTIRENLR"
     gene            1544..2470
                     /locus_tag="PLW15_01325"
     CDS             1544..2470
                     /locus_tag="PLW15_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33072.1"
                     /translation="MPAPVITASSLTKKYKDFVAVDGISFEVAPGESFGLLGPNGAGK
                     STTMRMVGAVSTRTSGDLSILGLDPNQHGPSIRSQLGVVPQADNLDTELRVRDNLLVY
                     GRYFGLSRSHIATRADELLEFAQLSDRSKAKIDDLSGGMKRRLTIARALINDPRILLL
                     DEPTTGLDPQARHILWDRLFRLKEQGTTLVLTTHYMDEAEQLCDRLVVVDKGTIMAEG
                     APAALIREHSSREVLEVRFGSERNATAADELAGIGDRLEVLPDRILIYATSGEEALVE
                     LTRRGLEPITSLVRRSSLEDVFLKLTGRTLVE"
     gene            2467..3282
                     /locus_tag="PLW15_01330"
     CDS             2467..3282
                     /locus_tag="PLW15_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33073.1"
                     /translation="MSADISPLQTGARARRWGAWYVAEHKIRSMRGYIGTLVATAIGT
                     PFLYLFAFGVGLATLITGNVGPVPGVTYLQFVAPALLATAGLLVAMEEYTFGILLGFK
                     WNAIFIGMNAAPISGRQIINGVMLFVGLRMLVTTSVYYVMMLLFGAVEPGWSALTIVA
                     GLLCGFAFSPVAAYAATIQEDRGQFAILQRVIILPLTLFSGTLFPLTQLPIFLQWIGW
                     LSPLWHASELGRQFVYGPTEPIWLTVAHIVYLVGLGVLGWQLCVRIATRRLDK"
     gene            3291..4100
                     /locus_tag="PLW15_01335"
     CDS             3291..4100
                     /locus_tag="PLW15_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33074.1"
                     /translation="MTPPTRSEAGLRSLYAGNARAVVGRGLLATRSTNWAIILTGVFE
                     PIFYLLALGLGLGSYITGVTDAQGNVIPYAAFIAPALLAVSAMNGAIYDSTWNVFFKM
                     HFGKLYQGMLATSLGPLDVALGEISLALLRGAAYGTTFLLVMTALGLNLSWTAILALP
                     AILVIAFGFASLGMGITSYLKSFQQMEWVQFVMLPMFLFSATFYPLTVYPEPIQWFIQ
                     ALPLWHGVELVRGLTTGALTPAMLLHLLYFLVMIAVGLVFTTRRLKALFLD"
     assembly_gap    4104..4203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4204..5475)
                     /locus_tag="PLW15_01340"
     CDS             complement(4204..5475)
                     /locus_tag="PLW15_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33075.1"
                     /translation="MTAPTLPPAFTPFAEPKRKVTAGWIALFATAWFGIWMAQLTPIQ
                     LLLPQQIDDLLSPEDWTQSVVAFGIISGIAGACALLAYPITGALSDRTTSRFGRRRPW
                     IFIGTLVFAAALFLLGIQTTIVGIGVFWSLALIGFCMLSAALTATISDQVPVNQRGFV
                     SGWVSAPQAVGTVLGLALVIGLALNALTGYALMSVLLIVLVLPFLLVVPDEVLPKVLR
                     PPFTVSALVKGFWINPIAFPDFGWTLLSRILVNIGNALGTTLLLYFIMFGLGRVDTAQ
                     DDLLALTGVYLITFIIAALGFGKLSDVIGQRKPFVYAAAYLQGLAALIIAFVPELTIT
                     FVAAGLLGLGYGCFMAVDQALATQVLPNSHTRGKDLGIMNIATTVPQALAPLLGAWLV
                     ALFAGFMGLFIVSALAAVLGGFAIMPVKKVR"
     gene            complement(5492..5887)
                     /locus_tag="PLW15_01345"
     CDS             complement(5492..5887)
                     /locus_tag="PLW15_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33076.1"
                     /translation="MKALIALATAALLLTGCSAQPVAPNSDIASLTFHQYQAIENFDD
                     SEYTQDDPTEVARFVSLLDEFGVVPGVTVTADQDECAGGLSSTVRVDYSGGESADMFI
                     ASCGKPDYDRFNQQANELFTEWRIDVSAQ"
     gene            complement(5913..6383)
                     /gene="ribH"
                     /locus_tag="PLW15_01350"
     CDS             complement(5913..6383)
                     /gene="ribH"
                     /locus_tag="PLW15_01350"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOT33077.1"
                     /translation="MSGEGHPVLDTDGTGLKVTIVSGLWHHAIADGLLAGARRVLEAS
                     GAEVTEVQVPGSFELPVASKVALDAGADAVVALGVIVRGGTPHFEYVSAAATDGLTRV
                     ALDTGKPVGFGVLTLDDEQQGLDRAGLPGSKEDKGAEAAEAALATAIVLRKIRG"
     gene            complement(6385..>7098)
                     /gene="ribA"
                     /locus_tag="PLW15_01355"
     CDS             complement(6385..>7098)
                     /gene="ribA"
                     /locus_tag="PLW15_01355"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOT33078.1"
                     /translation="VTIEALIEYLQEWHCDTDVPMPVHIPETSRVIFEVETTVPTEHG
                     PFRFRAYRDRQTGADHLAIISGNPTNGTLVRVHSECLTGEVFDSEKCECGPQLDAALE
                     LIQRDGGIVIYMRGHEGRGIGLINKLKAYRLQEDGLDTLDANLALGFPADGRDYGAAV
                     AILDDLGISEVRVITNNPEKLRQLGDRGITIVERVPLVVGVGEFNEKYLEAKRDRMGH
                     VLPTDDVLDAALTANRKDS"
     assembly_gap    7099..7198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7199..7503)
                     /locus_tag="PLW15_01360"
     CDS             complement(<7199..7503)
                     /locus_tag="PLW15_01360"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOT33079.1"
                     /translation="MSLADIPAAIAALAEGRPVIVVDNESRENEGDFIMAAQFATQEH
                     LAWMIRHSSGFICAPMTNELADRLELPYMVMDNQDPLGTAYTVTVDAADRRSTGISAG
                     "
     gene            complement(7500..8102)
                     /locus_tag="PLW15_01365"
     CDS             complement(7500..8102)
                     /locus_tag="PLW15_01365"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOT33080.1"
                     /translation="MFTGIIEELGEVVAWDGLGDAARITVRAPIAVSDARHGDSISVS
                     GVCLTVIDQGPDWFTADVMAETIAMSTLAGVAPGDRVNIERAAQVNDRLGGHIVQGHI
                     DGTSTLLSIEQGSAWRVLRFSLSADAAPLVVRKGSIAVDGVSLTVSAVGDDWFEVSLI
                     PETLAVTTLGSKDVGDRVNIETDILARHVARLMQFERSQP"
     gene            complement(8199..9209)
                     /gene="ribD"
                     /locus_tag="PLW15_01370"
                     /pseudo
     CDS             complement(8199..9209)
                     /gene="ribD"
                     /locus_tag="PLW15_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     regulatory      complement(9282..9426)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            10077..10550
                     /locus_tag="PLW15_01375"
     CDS             10077..10550
                     /locus_tag="PLW15_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33081.1"
                     /translation="MDALAQTVRCSPPHLALASIENALFLGKVDGSGIADLFAGLGSE
                     HQFLRARIDPRSESGQETVLRTALEDAGLRSEIQVLIPGVGRVDGVVEGRLIWEADSR
                     LAHDGWELHVRDRDRDIDAARLGYMSLRPAYNRTMHRTNEVVDAIRHLLDSTRRP"
     gene            complement(10698..11405)
                     /locus_tag="PLW15_01380"
     CDS             complement(10698..11405)
                     /locus_tag="PLW15_01380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT33082.1"
                     /translation="MTSVVLFDLDDTIFAHRLAVDRGIASHLAASGVTTVDAAEIARW
                     TELEEHHYHRYLTGELDYLGQRRARARDFAAPYGLRFTHDSEAEGWFEEYLDHYRAAW
                     SLYDDTLPCLDALAERGVRLGIITNGEEHFQLPKLERMGLADRFEHVIASGALGITKP
                     DRRIFEFACEKFGVPVSDALYVGDRLATDAVGARDAGLRGVWLVRDGAASGGEVASAE
                     ASGVTVITSLAQLPALV"
     gene            complement(11402..12409)
                     /gene="trpS"
                     /locus_tag="PLW15_01385"
     CDS             complement(11402..12409)
                     /gene="trpS"
                     /locus_tag="PLW15_01385"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT33083.1"
                     /translation="MTTKPRLFSGMQPSAESLHLGNYMGALLQWRDMQLTHDAVFCVV
                     DLHAITVPQDPALLRQQTRRTAAQYIAAGIDPSVSTLFVQSHVAAHPQLAWVLNTITG
                     FGEAGRMIQFKDKSAKQGADSASVGLFTYPILQAADILLYDATVVPVGEDQRQHIELT
                     RDLAMRFNSRFGETFVVPEVQILKETAKVYDLQEPDSKMSKSAASENGVLWLLDEPSK
                     TAKKIKSAVTDAEREIRFDRAEKAGVSNLLTIHSVLSGRSVADLEVEFEGRGYGDLKA
                     EVADVVVSTFEPIRTRALELLDDPAELDRILAANADRAAQIADATLAKVYDRVGFLPR
                     A"
     gene            complement(12503..13342)
                     /locus_tag="PLW15_01390"
     CDS             complement(12503..13342)
                     /locus_tag="PLW15_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT33084.1"
                     /translation="MVLRIASVNTNGVRAAYRKGMGDWLAARNVDILALQEVRASTED
                     LEQLLGPEWDILHDAATAKGRAGVALASRRRASIHRVAFGADDFDSAGRWIEADYEVG
                     DSIVTVVSTYVHSGEVDTPKQVEKYKFLDAMTERMPQLAAHSPLAVLMGDLNVGHREL
                     DIKNWKGNRKNAGFLPQERAYFDRFFGPAGEPVEGVDGSVAPGLGWVDVGRRWAGEVD
                     GPYTWWSNRGQAFDTDTGWRIDYHVATPALADTVTAYSVDRAAAYDQRWSDHAPVVVD
                     YDV"
     gene            complement(13352..14317)
                     /locus_tag="PLW15_01395"
     CDS             complement(13352..14317)
                     /locus_tag="PLW15_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT33085.1"
                     /translation="MTVRKRFAAVVARVQALKPVRVFDRYARSRGPLLSAGLSYQAIF
                     AVFGGIWVVFAIAGAVIASRPTLQRALFDLIETSIPGLIDDGSGTGAIDVENLFSAGI
                     FGWTGVIAAGVLLFTALGWLASGRDAVRAMFALPAAGTNPVLLKLKDLGLAVAFGLAI
                     LVSAVLTVLATSFLGSVFVWLGVDQRSDAAALAIRAAVLLIVLGLDTLVLGTFYRVVS
                     GIDIPFRLLAQGTLLAAVALGVLKVLGTSLLGGATSNPLLASFAVIIGLLLWFNLVCQ
                     VILVGAAWIAVSADDAGVDLTGKKKRAQAKTTAKQSAAKAVVTHQ"
     gene            14379..>14625
                     /locus_tag="PLW15_01400"
     CDS             14379..>14625
                     /locus_tag="PLW15_01400"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOT33086.1"
                     /translation="MELHGVGVGRGVAVGPILRMPDPLPEPVAGPHAGDPAAEYAHVE
                     KSLAAVAEELAAKGAKAGGEARAVLEAASMMAQDPTLT"
     assembly_gap    14626..14725
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14726..15871
                     /locus_tag="PLW15_01405"
     CDS             <14726..15871
                     /locus_tag="PLW15_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOT33087.1"
                     /translation="ELDKVLALITRDGGPTSHTAILARSKSIVAVVGVTGALDLTDGT
                     LVIADAATGVIVSDPTPTAIAAAEARIVERAALAVAPITDGALGDGTKIPLLANLGSP
                     KDAEAAVALGAEGVGLFRTEFLFLDSTTAPTVAEQARQYIDLLQHFPGKKVVVRALDA
                     GADKPLSFLNAAHEENPALGLRGLRALRANQQILADQLQALVNAQAATTADLWVMAPM
                     VSDAEETTWFVDYGKKLGLKTVGVMAEVPSLAVVADQVAEVSDFVSIGTNDLTQYTLA
                     ADRMLGSVASYQDPWHPAVLRLVKMLGDAGAAAGKPVGVCGEAAADPKLAVVLVGLGC
                     TTLSMTPAALAEVRAELRTVTLKEAQARAARALAATTAADARDSGSS"
     gene            complement(15918..16397)
                     /locus_tag="PLW15_01410"
     CDS             complement(15918..16397)
                     /locus_tag="PLW15_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33088.1"
                     /translation="MFPFMAAALALALSFAAPVAAPAPDVPTTSVVLAPRMASPVAER
                     RAPVIEWTITVDTIGYQDALDACLWTRMDFDGVVPIVGAHNFCGGDIVLDMKVGEIVH
                     LLGAGVDGTYVVTGDRQVYPGDDAIEATAGLDSPVLLQTCYWDVDQGMRLVALSQLG"
     gene            complement(16597..17337)
                     /locus_tag="PLW15_01415"
     CDS             complement(16597..17337)
                     /locus_tag="PLW15_01415"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOT33089.1"
                     /translation="MENQPHVPEVIPTFTATLIIRRFDPEVDDEPRWQDFDVELRGTD
                     RVLDALHKIKWEQDGSLTFRRSCAHGVCGSDAMRINGRNRLACKTLIKDLDISKPIYV
                     EAIKGLPLEKDLIVDMEPFFESYRSINPFLMASGSPDKERKQSPVERARFDDTTKCIL
                     CAACTSSCPVFWTDGQYFGPAAIVNAHRFIFDSRDEGAQVRLDILNDKEGVWRCRTTF
                     NCSEACPRGIQVTQAIAEVKMAVLRGKP"
     gene            complement(17357..>18871)
                     /gene="sdhA"
                     /locus_tag="PLW15_01420"
     CDS             complement(17357..>18871)
                     /gene="sdhA"
                     /locus_tag="PLW15_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOT33090.1"
                     /translation="LAKEAIDAVIDLENMGLPFNRTPDGKIDQRRFGGHTREHGKAPV
                     RRACYAADRTGHMILQTLYQNCVKLGINFFNEFYVLDLVMTKVDGKDQPSGVVAYELA
                     TGELHVFQGKAIIFATGGFGKIYKTTSNAHTLTGDGVGIIWRKGLPLEDMEFYQFHPT
                     GLAGLGILLSEAARGEGAILRNASGERFMERYTPTLKDLAPRDIVARSMALEVREGRG
                     AGPDKDYLYLDITHLEPAVIDEKLPDITEFARTYLGVEPYTEPVPVLPTAHYAMGGIP
                     TNVSAEVLRTNTEVVPGLYAAGECACVSVHGSNRLGTNSLLDINVFGKRAGKNAVEYV
                     KKTKQFTPLPENPAAGVQKILDMVRSGDGTERVAVIRKELQDSMDRNAQIFRTDESLE
                     EVTKLIETLRGRYKNIQLQDKGKRFNTDLLEAVELGFLLDLAEVLVYSARSRKESRGG
                     HMREDYPDRDDKNFLVHTMAYLVGDPESKEAGDHIKIDWKPVVITNYPPMERKY"
BASE COUNT         2963 a         6176 c         6372 g         3060 t
ORIGIN      
        1 cgcaggttct cgcggatcgt gcccgccagc accggggcat cctgttcgac gtagccgatc
       61 tgcgcccgca gatccgtgcg gtcgaggccg cgaatgtcga tgccgccgag gcgcaccacg
      121 cccgcgttcg ggtcgtagaa gcgctcgatg agggcgagga tcgtgctctt gcccgcgccc
      181 gaaggtccga ccagcgcggt gcgcttgccg cgctcggcgg cgaaggagac accgcgcagc
      241 acggtgcggt ctgagtcggc gatcgcgctc gtcgagatct ggccctccag gtgctcggtc
      301 tcgacgagct cgacaacctc cttctcgacc tgctcgacga cggtgccctc tgggtagctg
      361 aactccacgt tctcgaaggt gatcgcggca tccgtctcga tggctgccgc gggaacgatc
      421 gcggcgtcgc cgtcggtctc gctcggcagc gagatgatct cctggatgcg ccccagcgcg
      481 ccgagcgcct ggttgaccga attgatcgca ccgaaggcct ggccgagcgg catgatcatc
      541 atgaacagga agaggatgaa cgacaccagg ctcgcgacgg tgatcgcacc gctggcgacc
      601 cggaagccgc cgacgccgag caccacgagc agcgcgacct gcagggcgat gcccgcgatc
      661 ggcacgacga gtgcggagat cttcgcaacc tggatgccca tgcgccaggc gccctcggca
      721 tccttctcga cggcggcgat ctcacgatcg gtggcattgg atgcccgcac cgtacgcacg
      781 gcgctcaagg accgctcgac cgacgccgcc aggtcgccga ccttctcctg ctgcttgcgg
      841 ctggccaccc ggatgcgccc ggacaggctc acgacggtga ccacggcgat ggcgaccacc
      901 agcagggtga gtccgagcag cagcggatcg atgatgagca tggcaacaag cgcaccgatg
      961 aagacgagcg ctccgccgat cgcgtcgacg agcccctgcg tgatgacggc gtagagcagc
     1021 gtggtgtccg acccgacgcg gctgactagg tcgcccgtgc ggcgcgtgtc gaactcgctg
     1081 atcggcagcc gcagcatccg gcgcaccaac tgccggcggg ccgacagcac gacgctggtt
     1141 ccggtgcgct gcagcaggta gtgctggaat ccgctcagca cgccagagat cacgaccagc
     1201 ccgacgagcg cccagacgag catgccgagg ggcttgttcg cctgcacgac agtgatgacc
     1261 tgactcacca gcagcggctg ggccagcgag gccgccgccg cgaggatgct cagcgcgacg
     1321 acgacactga gaatgccgcg gtgctcggcg aggtacggcc agagctggct gagcttcgcg
     1381 cgcggaccgg tatcgggcgt gcgcgagaag gggcttcggc ggctggtgtc gttcgctgtc
     1441 gtcatggtcc ctcaagtctc gctcaggttg cgggccgcct agtactccgt gcggggtaat
     1501 tcgcgggtgc cagtgtcgca cccccgggtt aggctcgatg actgtgccag cacctgtcat
     1561 caccgcgtcc tcgctcacca agaagtacaa ggacttcgtc gccgtcgacg gcatctcgtt
     1621 cgaggtggct ccgggggagt cgttcggcct gctcgggccc aacggtgcgg gcaagtcgac
     1681 cacgatgcgc atggtcgggg cggtgtcgac gcgcaccagc ggcgacctgt cgatcctggg
     1741 tctcgacccc aatcagcacg ggccgtcgat ccgctcccaa ttgggtgtcg tgccgcaggc
     1801 cgacaacctc gacaccgaac tgcgcgtgcg cgacaacctg ctggtctacg gccgctactt
     1861 cggactctcg cgctcccaca tcgccacgcg cgccgacgag cttctcgagt tcgcgcagct
     1921 gagcgatcgg tccaaggcca agatcgatga cctgagcggc gggatgaagc ggcgcctcac
     1981 catcgcgcgt gcgctgatca acgacccgcg catcctgctg ctcgacgaac cgacgaccgg
     2041 gctggacccg caggcgcggc acatcctgtg ggatcgactg ttccggctca aggagcaggg
     2101 cacgacactc gtgctcacca cccactacat ggatgaggcc gagcagctgt gcgaccggct
     2161 cgtcgtcgtc gacaagggca ccatcatggc cgagggcgca cccgccgccc tgatccggga
     2221 gcactcgagt cgcgaggtgc tcgaggtgcg gttcggctcc gagcgcaacg cgacggcggc
     2281 cgacgagctc gctggcatcg gcgaccgtct cgaggtgctg cccgatcgca tcctgatcta
     2341 tgcgaccagt ggcgaggagg ccctggtcga gttgacccgg cgtggcctcg agccaatcac
     2401 gagcctcgtg cggcgctcca gcctggagga tgtcttcctc aagctcacgg gtcggaccct
     2461 cgtcgaatga gcgccgacat cagcccgctc cagacgggcg cccgcgcacg ccgctggggc
     2521 gcgtggtacg tcgccgagca caagatccgc tcgatgcgcg gctacatcgg taccctcgtc
     2581 gcgacggcga tcggcacacc gttcctctac ctgttcgcct tcggtgtcgg tctggcgacc
     2641 ctcatcacgg gcaacgtggg cccggtgccc ggcgtcacct acctgcagtt cgtcgccccc
     2701 gcactcctcg cgaccgcggg gctattggtg gcgatggagg aatacacctt cggcatcctg
     2761 ctcggcttca agtggaacgc catcttcatc ggcatgaacg ccgcccccat ctccgggcgg
     2821 cagatcatca atggcgtcat gctgttcgtc ggcctgcgca tgctcgtcac gacgagcgtc
     2881 tactacgtga tgatgctgct gttcggcgcg gttgagcccg gctggagcgc gctgaccatc
     2941 gtcgcggggc tgctctgcgg cttcgccttc tcgccggtcg cggcctacgc cgcgacgatt
     3001 caggaggatc gcggccagtt cgccattctg cagcgcgtga tcatcctgcc gctgaccctg
     3061 ttctcgggaa cactcttccc cttgacccag ctgccgatct tcctgcagtg gatcggctgg
     3121 ctctccccgc tctggcacgc cagcgagctg ggccgtcagt tcgtctacgg cccgaccgaa
     3181 ccgatctggc tgacggtcgc gcacatcgtc tacctcgtcg gactcggcgt gctcggttgg
     3241 cagctctgcg tgcgcatcgc gacccggagg ctcgacaagt gacatccacc atgacccctc
     3301 cgacccggtc cgaggccgga ctccgctccc tgtacgcggg caacgcgcgg gcggtcgtcg
     3361 gccgcggtct gctcgcgacc cgaagcacca actgggcgat catcctcacc ggagtgttcg
     3421 agccgatctt ctacctgctc gcgctcggtc tcgggctcgg cagctacatc accggcgtga
     3481 cggatgccca gggcaacgtc atcccgtacg ccgcgttcat cgcgccggcg ctgctcgcgg
     3541 tctcggcgat gaacggtgcg atctacgact ccacgtggaa cgtcttcttc aagatgcact
     3601 tcggcaagct ctatcaggga atgctcgcca ccagtctcgg tccattggat gtcgcgctcg
     3661 gcgagatctc gctcgccctg cttcgcggcg cggcctacgg cacgacgttc ctgctggtga
     3721 tgacggcgct cgggctgaac ctgtcgtgga ctgccatcct cgcgttgccc gcgatcctgg
     3781 tgatcgcgtt cggtttcgcc agcctcggca tggggatcac gagctacctg aagtcgttcc
     3841 agcagatgga gtgggtgcag ttcgtgatgc tgccgatgtt cctgttctcg gcgacgttct
     3901 acccgctgac ggtgtatccc gagccgatcc agtggttcat tcaggcgcta ccgctctggc
     3961 atggcgtcga gctggtgcgc ggactcacga cgggtgcgct gacgccagcg atgctgctgc
     4021 acctgctgta cttcctcgtg atgatcgccg tggggctggt cttcacgacg cgtcgcctga
     4081 aggcgctgtt cctggactag aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnntcagcgc accttcttga cgggcatgat cgcgaagcca ccgagcactg ccgcgagggc
     4261 cgacacgatg aacagtccca tgaagcccgc gaacagggcg accagccacg caccgagcag
     4321 cggcgcgagc gcctggggca ccgtggtggc gatgttcatg atgccgaggt ccttgccgcg
     4381 ggtgtgcgag ttcgggagca cctgggtcgc gagggcctgg tcgacggcca tgaagcatcc
     4441 gtaaccgaga ccgaggagcc ccgcggcgac gaaggtgatc gtcaactcgg gaacgaacgc
     4501 gatgatcagg gcagcgagcc cctgcaggta ggccgcggcg tagacgaaag gcttgcgctg
     4561 gccgataacg tcgctgagct tgccgaatcc gagggcggcg atgatgaacg tgatgaggta
     4621 gacgccggtg agcgccagca ggtcatcctg agcggtatcg acgcgaccca gaccgaacat
     4681 gatgaagtac agcagcaagg tggtaccgag cgcgttgccg atgttgacga ggatgcggct
     4741 gagcagggtc cagccgaagt cggggaacgc gatcgggttg atccagaacc ccttcacgag
     4801 ggccgagacg gtgaacgggg ggcgcagcac cttgggcagc acctcgtcgg gcaccaccag
     4861 aaggaacggc agcacgagca cgatcaacag aaccgacatg agggcgtagc cggtgagcgc
     4921 gttcagggcc aaaccgatga caagcgcgag gccgagcacg gtgccgaccg cctgtggtgc
     4981 cgagacccag ccggagacga agccgcgctg gttgacggga acctggtcgg agatcgtcgc
     5041 cgtgagggca gcagagagca tgcagaaacc gatgagcgcg agcgaccaga agacgccgat
     5101 ccccacgatc gtggtctgga tgccgagcag gaagagcgcc gcggcgaaca ccagtgtgcc
     5161 gatgaagatc cacgggcggc ggcgcccgaa ccgcgaggtg gtgcggtcgg acagggcgcc
     5221 ggtgatggga taggcgagca gggcgcaggc gccggcgatt ccggagatga ttccgaaggc
     5281 gacgacgctc tgagtccagt cctcagggct gagcaggtcg tcgatctgct gcggcagcag
     5341 cagctggatg ggcgtgagct gcgccatcca gataccgaac caggctgtgg cgaacagggc
     5401 gatccacccg gccgtcacct tgcgcttggg ctcggcgaac ggggtgaatg cgggcggaag
     5461 cgtcggtgcg gtcaccgcgc cagattacgc gctactgggc gctcacgtcg atgcgccact
     5521 cggtgaacaa ttcgttggct tgctggttga atctgtcgta gtcgggcttg ccgcagctcg
     5581 cgatgaacat gtcggcgctc tcgccaccgc tgtagtccac tcgcaccgtc gaactcaggc
     5641 cgcccgcgca ctcgtcctga tcggcagtga cggtgacacc tggcacgacg ccgaactcgt
     5701 cgagcagact cacgaagcgc gcgacctcgg tggggtcatc ctgggtgtac tcggagtcgt
     5761 cgaagttctc gatggcctgg tactggtgga aggtcaggga tgcgatgtcg gagttcggcg
     5821 cgaccggctg cgccgagcat ccggtcagca gcagggctgc cgtggcgagc gcgatgagtg
     5881 ccttcatgcg cacgagacta gcgcgcgtgg gctcagccgc ggatcttcct cagcacgatc
     5941 gcggtcgcga gcgccgcttc ggccgcctca gcgcccttgt cctccttcga cccgggaagg
     6001 ccggcgcggt cgaggccctg ctgctcgtcg tcgagcgtga gcaccccgaa gccgacaggc
     6061 ttgccggtgt cgagcgcgac gcgcgtgagg ccgtcggtcg cggcggcgct gacgtactcg
     6121 aagtgaggcg ttcccccacg cacgatcacg ccgagcgcga cgaccgcgtc ggccccggca
     6181 tcgagggcga ccttgctcgc gaccggcagc tcgaagctgc ccggaacctg cacctcggtg
     6241 acttctgctc cggatgcctc aagcacccgc cgcgcgccgg ccagcaggcc atcggcgatc
     6301 gcatggtgcc acagtcccga gacgatggtg accttcagtc ccgttccgtc ggtgtcgagt
     6361 acgggatgtc cttcgccgct catatcagct gtccttccgg ttggcggtca gtgccgcatc
     6421 gagtacgtcg tcggtgggca gcacatggcc catccggtca cgcttggctt cgaggtactt
     6481 ctcgttgaac tccccgaccc cgacgacgag cggcacacgc tcgacgatcg tgatgccgcg
     6541 gtcgccgagc tggcgcagct tctcggggtt gttggtgatg accctcacct cggagatgcc
     6601 caagtcgtcg aggatcgcga ccgcggcgcc gtagtcgcgc ccgtctgcgg ggaagccgag
     6661 cgcgaggttc gcgtcaaggg tgtcgaggcc gtcctcctgc aggcggtagg ccttcagctt
     6721 gttgatgagg ccgatgcccc gaccctcgtg gccgcgcatg tagatgacaa tgccgccatc
     6781 gcgctggatc agttcgagcg cggcatccaa ctgagggccg cactcgcact tctcggagtc
     6841 gaacacctca cccgtgaggc actcggagtg cacgcgcacg agggtgccgt tggtcgggtt
     6901 gccggagatg atggcgaggt ggtccgcgcc ggtctggcgg tcgcggtagg cgcggaagcg
     6961 gaacgggccg tgctcggtcg gcaccgtcgt ctccacctcg aagatcaccc gggatgtctc
     7021 ggggatgtga acgggcatgg gcacgtcggt gtcgcagtgc cattcctgca ggtactcgat
     7081 gagcgcctcg atcgtgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     7201 cgcgctgatg cccgtgctgc ggcggtcggc ggcatccacg gtcacggtgt acgcggtgcc
     7261 cagggggtcc tggttgtcca tgaccatgta gggcagctcg agccggtcgg ccaactcatt
     7321 ggtcatgggt gcgcagatga agccggacga gtgtcgaatc atccacgcta gatgttcctg
     7381 agtcgcgaac tgggccgcca tgatgaagtc gccctcgttc tcgcggctct cgttgtcgac
     7441 cacgatcacg ggtcgaccct cagccagtgc ggcgattgcc gcggggatgt ctgccaggct
     7501 catggctggc tcctttcgaa ctgcatcagt cgtgccacat gacgggccag gatgtccgtc
     7561 tcgatgttca cgcggtcgcc cacgtctttg ctgccgagcg tcgtcacagc caacgtttcg
     7621 gggatgaggc tcacctcgaa ccagtcgtcc ccgaccgcgg acaccgtgag cgacaccccg
     7681 tcgacggcga tcgagccctt gcggacgacg aggggcgcgg catccgccga gaggctgaat
     7741 cgcagaacgc gccaggcgct gccctgctcg atggagagca gcgtcgacgt gccgtcgatg
     7801 tggccctgca cgatgtggcc gccgaggcgg tcattgacct gagccgcgcg ctcgatgttg
     7861 acgcggtcgc cgggcgcgac gcccgcgagg gtgctcatcg cgatcgtctc ggccatgacg
     7921 tcggccgtga accagtcagg gccctgatcg atcactgtca ggcacacccc gctgacggag
     7981 atggagtccc cgtggcgcgc gtcggagacc gcgatcggtg cgcgcacggt gatgcgcgcg
     8041 gcatccccga gcccgtccca ggcgacgacc tcgccgagct cctcaatgat tcccgtgaac
     8101 atggttacct ttcgtctcgg tggtcgagtt tcggtggtcg agcttgtcga gacccgagac
     8161 ccgagacccg agacccgaga cccgagcccg gccgcggtcg gccgcgccgt gacgaggatg
     8221 tcctcgccga gccgctcgac cgcagtgatg tgaagctcgc gcgcatcggc gatcgtgccg
     8281 accccgatgt cgccgagcgc gagcaggggg ccaccgagca gcttcggggc gaggtagatc
     8341 aggtactcgt cggcgagacc cgaggtcacg atcgcgctgg cgagggtggg tccgccctcg
     8401 acgtaggcgc ggcgcacgcc ctgctcgaac agccacgaca tgacctcgcg caggtcgcga
     8461 gtgcccgact cgagcacgcc gcgggggtgc tcccacagcc gggcgtcgcc gggaatcgcg
     8521 cgctcgccga tcacgacggg caacggctgg tgctcgcgca gctcgccgga ctcggtgcgc
     8581 gcggtgagcg tggggtcgtc ggcgagcacg gtgccggtgc ccacgaggat cgcatcggat
     8641 gccgcgcgct gctcatgcac tcgctcgcgc gactcggggc cggtgatcca ctggctggaa
     8701 ccgtcggcgg ccgccgcccg accgtcgagg gtggacgccc acttgacggt gacccagggc
     8761 gtgcggcgac gggttgccgt cagccagggg aggaggaact cggtcgcctc gtcggcgagc
     8821 acaccctgct cgacctcgac gcccgcggcc cgcagtcgtt cggcgccgcc accctcgacg
     8881 ggcccggggt cggccacggc atagaccacc ttcgcgacgc ccgccgcgat gagtgcctcg
     8941 ctgcacggac cggttcggcc ggtgtggttg cacggctcca gggtcacgac cgccgtcagg
     9001 ccgcgcgcac tgtcgatctt cgagagcgcg tcgatctcgg cgtgcggggt gccggcaccg
     9061 tggtgccagc cctccgagac gattgttccc tccgggtcga gcactacgca gccgacctgg
     9121 ggattgccac cgacgagcgg tccgcgggcg gcgagttcga gcgcgcgccg catggcggcc
     9181 tcgtaacgct catcgcctgg ggcggtcatc cggtgtcctc gtctgctcac gacgaactcc
     9241 gggggtggtc gaacgacccg gtgcacggca acgccgaaca ccccgtgcgc ctctcatccg
     9301 gactgaggtg tcttgcgaca ccattaccgt cggtcccgga gttccaccag gtcaaccctc
     9361 cccttgcggg tcgggctcgc ggactatcac cgccggttcg gactttcacc gaccccggag
     9421 cacgtactac ttgcttacga gtgtaccaac gcgggtgttg cgcgagtatt cccggggcgg
     9481 ccgcgccgcc gccgcgcaac ccaccgttgt cgataactcc tgcgaaccag gcccggaggc
     9541 cagagggtgc cgcggagaac cggtgctaat tctcgccgct cactgtcgat tgcaggagtt
     9601 atcgacaggg gcagctctcc accggcacgc ccgtcacgcc accatgggcg ggcgtcggct
     9661 ccgagactcg tagggtgaca tggagtgctg cgttcggcga actcgggttg accgttgcat
     9721 ctcgggagca attgttctcg atcggggcca cgtcgcgaat gctcaccggc ggtgtgcggt
     9781 ccggagttct cctgcgggtt cgtcgcgacc actacgccct tccgtcgacc gacctgcacg
     9841 tcctccgggc ggttcgggtt gggggtcggc tcggctgtat gacggctctg cgggccttcg
     9901 ggatcttcgg cttcgaaagc catgtcacgc acatccacct tccacgtgag ctttcccgag
     9961 ctcgtagccc acacgaccgc cgccatcacc tgactcgcgc caatcgcgca ggtactgaac
    10021 ttcattggtc ggctcttctc gagcctgagg ccggttccga ggtcagcgtg ggagtcgtgg
    10081 acgccctcgc acagacggtc aggtgcagtc cgccccacct ggccctcgct tcgatcgaga
    10141 acgcactctt cctcggcaag gtcgacgggt ccggcatcgc tgacctattc gccgggcttg
    10201 ggtccgagca ccaattcctc cgagctcgca tcgaccctcg cagcgagtcg ggccaggaga
    10261 cggttctgcg caccgccctc gaagacgcgg gactgcgttc cgagatccag gtgctcatcc
    10321 ccggagtcgg gcgtgtcgac ggggtggtcg agggacgtct catctgggag gccgacagca
    10381 gactcgccca cgacggatgg gagttgcacg tgcgtgatcg cgatcgtgac atcgacgcgg
    10441 cccggctggg gtacatgtcc ctccgacccg cctataaccg caccatgcac cgcacgaacg
    10501 aggtcgtcga tgcgatccgg cacctgctgg actccaccag gagaccgtga cagccgcagg
    10561 tagccacctc ctctcggttg ataactcctg cagttcgggg acaccgaaca cggtgaacgc
    10621 tcgcgttggc ggttggccca ggcgctccga acacgattgc aggagttatc gacaggagac
    10681 gtgccgaggt cgagccgtca gacgagggcg ggcagctgcg cgagcgaggt gatgaccgtg
    10741 actccggatg cctccgccga agcaacctcg ccgcccgaag cagccccgtc ccgcaccagc
    10801 cacaccccgc gcaggcccgc gtcgcgagcc cccaccgcgt cggtcgccag gcgatccccg
    10861 acgtacagcg catcggacac gggcaccccg aacttctcgc acgcgaactc gaagatgcga
    10921 cggtccggct tggtgatgcc gagggcaccg ctggcgatga cgtgctcgaa ccggtctgcg
    10981 aggcccatgc gctcgagctt cggcagctgg aagtgctcct cgccgttggt gatgatgccg
    11041 aggcgcacac cgcgctccgc cagggcatcg aggcacggca gcgtgtcgtc gtagagcgac
    11101 caggcagcgc ggtagtggtc gaggtactcc tcgaaccagc cctcggcctc gctgtcgtgg
    11161 gtgaaccgca gcccgtacgg cgcggcgaag tcgcgcgcgc gagcacgccg ctggccgagg
    11221 tagtcgagct cgcccgtcag gtagcggtgg tagtggtgct cctccagctc ggtccagcgc
    11281 gcgatctctg cggcgtcgac cgtcgtgacc ccggacgccg caaggtgcga ggcgatgccg
    11341 cggtcgacgg ccagccgatg ggcgaagatc gtgtcatcca ggtcgaagag caccaccgag
    11401 gtcatgcgcg tggcaggaat cccacgcggt cgtacacctt cgcgagcgtg gcgtcggcga
    11461 tctgggcggc acggtcggcg ttggcggcga ggatgcggtc gagttcggcc gggtcatcca
    11521 gcagttcgag tgcccgcgtg cggatcggct cgaacgtcga cacgacgacg tcggccacct
    11581 ctgccttgag gtcgccatag ccgcgaccct cgaactcgac ctcgaggtcg gcgaccgagc
    11641 gtcccgagag cacggagtgg atcgtgagga ggttggagac gcccgccttc tcggcccggt
    11701 cgaagcggat ctcgcgctcg gcatccgtca ccgccgactt gatcttcttc gcggtcttcg
    11761 acggttcgtc gagcagccag agcacgccgt tctcagaagc cgccgacttg ctcatcttgg
    11821 agtcgggctc ctgcaggtcg tagaccttcg cggtctcctt gagaatctgc acctccggca
    11881 cgacgaacgt ctcgccgaac cgcgaattga agcgcatcgc gaggtcgcgg gtgagttcga
    11941 tgtgctggcg ctggtcctcg ccgacgggca cgacggtcgc gtcgtagagc aggatgtcgg
    12001 ccgcctgcag gatcgggtag gtgaacagac cgaccgaggc cgagtcggca ccctgcttgg
    12061 ctgacttgtc cttgaactgg atcatgcgcc cggcctcgcc gaacccggtg atcgtgttga
    12121 gcacccaggc gagctgcgga tgcgccgcca catgtgattg cacgaacagg gtcgagacgc
    12181 tcgggtcgat tcccgcggcg atgtactggg cggcggtgcg ccgcgtctgc tgccgcagca
    12241 gcgcgggatc ctggggcacc gtgatcgcgt gaaggtccac gacgcagaac acggcgtcgt
    12301 gggtgagttg catgtcccgc cactgcagca gcgcacccat gtagttgccg aggtggaggg
    12361 attcggcgga gggctgcata ccggagaaga gacggggctt ggtggtcacg cccctagtct
    12421 ttcagaccgg gccggtcgag taggccgcag gccgtatcga gaccaaggtt tcgatacgcg
    12481 gctgcgccgc tactcaaccg gctcacacgt cgtagtccac caccacgggc gcgtggtcgc
    12541 tccagcgctg gtcgtaggcg gccgcacggt ccacgctgta ggcggtgacg gtgtcggcca
    12601 gcgctggggt ggcgacgtgg tagtcgatgc gccagccggt gtcggtgtcg aaggcctggc
    12661 cgcggttcga ccaccaggtg tacgggccgt cgacctcgcc cgcccaacgg cggccgacat
    12721 ccacccaccc gagccccggc gcgaccgaac cgtcgacgcc ctcgaccggc tcgccagcgg
    12781 gtccgaagaa acggtcgaag tacgcgcgtt cctggggcag gaacccggcg ttcttgcggt
    12841 tgcccttcca gttcttgatg tcgagctcgc ggtggccgac gttcagatcg cccatgagca
    12901 cggccagcgg gctgtgggcg gcgagctgcg gcatccgctc ggtcatcgca tcgaggaact
    12961 tgtacttctc gacctgcttg ggggtgtcga cctcgcccga gtgcacgtag gtgctcacga
    13021 cggtcacgat cgagtcgccg acttcatagt cggcctcgat ccatcggccg gcgctgtcga
    13081 agtcgtcggc accgaaggcc acacggtgga tgctcgcccg gcgccgcgag gcgagggcga
    13141 ctcccgcgcg gcccttcgcc gtggcggcgt cgtgcaggat gtcccactcc ggcccgagca
    13201 gctgctcgag atcctcggtg gatgcccgca cctcctgcag cgcgaggatg tcgacgttgc
    13261 gcgccgcaag ccagtcgccc atgcccttgc ggtaggcggc gcgaacgccg ttcgtgttga
    13321 cggacgcgat gcgaagaacc atgcgtcgac cctactggtg ggttacgaca gccttcgcgg
    13381 ccgactgctt ggccgtggtc ttcgcctgcg cccgcttctt cttgccggtc agatcgaccc
    13441 ccgcgtcgtc agcgctcacg gcgatccacg cggccccgac caggatcacc tggcagacca
    13501 ggttgaacca cagcagtaga ccgatgatga ccgcgaacga cgccagcagg gggttgctgg
    13561 tcgcgccgcc gagcaggctc gtgcccagca ccttgagcac gcccagggca accgcggcca
    13621 gcagggttcc ctgcgcgagc aggcggaacg ggatgtcgat gcccgagaca acccggtaga
    13681 aggtgccgag cacgagggtg tcgaggccga gcacgatgag cagcaccgcg gcgcggatgg
    13741 cgagggccgc ggcatccgat cgctggtcga cacccagcca gacgaagaca ctgcccagga
    13801 acgacgtcgc aagcacggtc agcacggccg agacgaggat cgccaggccg aaggcgacag
    13861 cgagcccgag gtctttgagc ttgagcagca ccgggttggt tcccgccgcg ggcagtgcga
    13921 acatcgcgcg cacggcgtcg cgacccgacg ccagccaccc gagggcggtg aacagcagca
    13981 cgcccgcggc gatgacgccc gtccagccga agatgcccgc agagaacagg ttctcgacgt
    14041 cgatcgcgcc ggtgccgctg ccgtcatcga tgaggcccgg gatgctcgtc tcgatgagat
    14101 cgaacagggc gcgttgcaga gtgggtcgcg atgcgatcac ggcgccggcg atcgcgaaca
    14161 ccacccagat tccgccgaac acggcgaaga tcgcctgata cgacagcccg gccgagagca
    14221 ggggaccccg gctcctcgcg tagcgatcga acacgcgcac cggtttgaga gcctgcactc
    14281 gcgcgaccac agcagcgaac cgcttcctca ccgtcatggc cccagcgtag ctagagtgac
    14341 gacgggcaac tttagttctc gagaggacct tgacaacgat ggagctgcac ggtgttgggg
    14401 tcgggcgcgg tgtcgcggtc ggcccgatcc tgagaatgcc cgatccgctg cccgagccgg
    14461 tcgccgggcc gcacgctggc gacccggccg cggagtacgc ccacgtggag aagtccctcg
    14521 ccgccgtggc cgaggagttg gctgcgaagg gcgccaaggc gggaggtgag gcgcgggcgg
    14581 tgctggaggc ggcatccatg atggcccagg accccacgct caccgnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnngagct cgacaaggtg ctcgccctca tcacgcgcga
    14761 cggcggcccg acgagtcaca ccgcgatcct cgcgcggtcg aagtcgatcg tcgcggtcgt
    14821 cggcgtgacc ggcgcgctcg atctcacgga cggcacgctc gtgatcgcgg atgccgcaac
    14881 cggcgtcatc gtgagcgatc cgacccccac ggccatcgcc gccgccgagg cccgcatcgt
    14941 cgagcgcgcc gcgctcgccg ttgcgccgat cacggatggc gccctaggcg acgggaccaa
    15001 gattccgttg ctggccaacc tcggctcgcc gaaggacgcc gaggcggccg tcgcactcgg
    15061 ggccgagggc gtcggtctgt tccgcaccga gttcctcttc ctcgactcga cgaccgctcc
    15121 gaccgtcgcc gagcaggccc gtcagtacat cgacctgctc cagcacttcc ccggcaagaa
    15181 ggtcgtcgtg cgcgcgctcg acgcgggagc cgacaagccg ctgagcttcc tcaacgccgc
    15241 ccacgaggag aacccggccc tcggactgcg cggccttcgg gcgctgcggg cgaaccagca
    15301 gatcctcgcc gatcagctgc aggcgctcgt caacgcgcag gctgcaacga ccgccgatct
    15361 ctgggtgatg gcacccatgg tctccgatgc cgaggagacc acgtggttcg tcgactacgg
    15421 caagaagctc ggtctcaaga ctgtgggtgt gatggccgag gtgccgtcgc tggccgttgt
    15481 ggccgaccag gtggccgagg tgagcgactt cgtgtccatc ggcaccaacg acctgaccca
    15541 gtacacgttg gcggcggacc gcatgctcgg ctcggttgcc tcgtaccagg acccgtggca
    15601 tccggccgtg ctcaggctcg tgaagatgct cggggatgcc ggtgcagcag caggcaagcc
    15661 ggtgggcgtc tgcggtgagg ccgcggccga cccgaagctc gcggtggtgc tcgtgggtct
    15721 cggatgcacg accctctcga tgacgccggc cgcactcgcc gaggtacgcg ctgagctcag
    15781 gaccgtcacg ctcaaggagg cgcaggcccg ggcggcgcgc gctctcgcgg cgacgaccgc
    15841 cgccgacgcg cgagattcag gaagcagcta gccccaagat ttcacccgaa gccgcgtcat
    15901 gctagacagt ctgtcgacta gcccagctga ctcagcgcga cgaggcgcat cccctggtcg
    15961 acatcccagt agcaggtctg cagcagcacg ggggagtcga ggcctgctgt ggcctcgatc
    16021 gcgtcgtcgc ccggatacac ctggcgatcg ccggtaacga cgtaggtgcc gtcgacaccg
    16081 gcaccgagga gatgcacgat ctcgccgacc ttcatgtcga gcacgatgtc gccaccgcag
    16141 aagttgtggg cgccgacgat gggcacgacc ccgtcgaagt ccattcgggt ccagaggcag
    16201 gcgtcgagcg cgtcctggta cccgatcgtg tcgacggtga tcgtccactc gatgacgggt
    16261 gcccggcgtt cggcgaccgg ggacgccatc cgtggcgcga gcacgacact ggtggtcgga
    16321 acgtcgggtg cgggggctgc tacgggcgcg gcgaagctga gggcgagcgc gagggcggcc
    16381 gccatgaagg ggaacatcgg gtgggggcga ttccgcgcag aagttccact gcgctgcatt
    16441 cgggggccgc agggggatgc ggaagatcag cgaccgtacg ggtgtaggtg ctgcgattcg
    16501 gggtgccggt gggttggccg actcgccaac actacgtgct ttgccctagg aggtcaaccg
    16561 atctgtctgt ccccctcgaa gaatccgggg atacagctag ggcttgcccc gcagcacggc
    16621 catcttgacc tcggcgatcg cctgcgtcac ctggatgccg cgggggcacg cctcggagca
    16681 gttgaaggtc gtgcggcagc gccacacccc ctccttgtcg ttgaggatgt cgaggcgcac
    16741 ctgggcgccc tcgtcgcgcg agtcgaagat gaagcggtgc gcgttcacga tggcggccgg
    16801 gccgaagtac tggccgtcgg tccagaacac cgggcagctc gacgtgcacg cagcgcagag
    16861 gatgcacttg gtcgtgtcgt cgaagcgcgc gcgctcgacc gggctctgct tgcgctcctt
    16921 gtcgggcgag cccgacgcca tgaggaacgg gttgatcgag cggtagctct cgaagaacgg
    16981 ctccatgtcg acgatgaggt ccttctccag cggcaggccc ttgatggcct ccacgtagat
    17041 gggcttcgag atgtcgaggt ccttgatgag cgtcttgcag gccaggcggt tgcgaccgtt
    17101 gatgcgcatc gcatccgatc cgcagacgcc gtgcgcacac gagcgacgga acgtcagcga
    17161 cccgtcctgc tcccacttga tcttgtgaag cgcgtcgagc acgcggtcgg tgccgcgcag
    17221 ctcgacgtcg aagtcctgcc agcgcggttc gtcatccacc tcgggatcga agcgacggat
    17281 gatcagggtc gccgtgaagg tcgggatgac ctcgggcacg tgcggctggt tctccattac
    17341 ggctgcggtg ctcatgtcag tacttgcgct ccatcggtgg gtagttcgtg atcacgaccg
    17401 gcttccagtc gatcttgatg tggtcgcccg cctccttgga ttcggggtcg ccgaccaggt
    17461 aggccatggt gtgcacgagg aagttcttgt catcccggtc ggggtagtcc tcgcgcatgt
    17521 ggccgccgcg gctctccttg cggctgcgcg ccgagtagac gagcacctca gcgaggtcga
    17581 gcaggaagcc gagctccacg gcctcgagca ggtcggtgtt gaagcgcttg cccttgtcct
    17641 gcagctggat gttcttgtac cggccgcgaa gggtctcgat gagcttggtg acctcttcga
    17701 ggctctcgtc ggtgcggaag atctgcgcgt tgcggtccat ggagtcctgc agctccttgc
    17761 ggatgacggc gacacgctcg gtgccgtcgc cgctacgaac catgtcgagg atcttctgca
    17821 cacccgccgc cgggttctcc ggcaggggcg tgaactgctt ggtcttcttc acgtactcga
    17881 ccgcgttctt gccggcacgc ttgccgaaga cgttgatgtc gagaagcgag ttggtgccca
    17941 ggcggttcga cccgtgcact gagacgcagg cgcactcgcc ggccgcgtac agacccggca
    18001 cgacctcggt gttggtgcgc agcacctcgg cactgacgtt ggtcgggatg ccgcccatcg
    18061 cgtagtgcgc cgtcggcagc accggcacgg gctccgtgta cggctcaaca ccgagatagg
    18121 tgcgggcgaa ctcggtgatg tccggcagct tctcgtcgat gacggccggc tcgaggtggg
    18181 tgatgtcgag gtacaggtag tccttgtcgg gaccggcacc gcgaccctcg cgcacctcca
    18241 gggccatcga cctggcgacg atgtcgcgcg gcgcgaggtc cttcagggtc ggggtgtagc
    18301 gctccatgaa gcgctcgccg gaggcgttgc gcaggatcgc gccctcgcct cgagccgctt
    18361 cggagagcag gatgccgagc cccgcgagtc cggtcgggtg gaactggtag aactccatgt
    18421 cctcgagcgg caggcccttg cgccagatga ttccgactcc gtcaccggtc agggtgtggg
    18481 cgttggaggt ggtcttgtag atcttgccga agccaccggt ggcgaagatg atcgccttgc
    18541 cctggaagac gtgcagctcg ccggtcgcga gctcgtaggc gacgacgccc gagggctggt
    18601 ccttgccgtc gaccttggtc atcacgaggt cgagcacgta gaactcgttg aagaagttga
    18661 tgcccaactt gacgcagttc tggtagagcg tctgaaggat catgtgaccc gtgcgatcgg
    18721 cggcgtagca ggcgcgacgc accggcgcct tgccgtgctc gcgggtgtga ccaccgaagc
    18781 ggcgttgatc gatcttgccg tcgggcgtgc ggttgaacgg caggcccatg ttctcgaggt
    18841 cgatgaccgc gtcgatggcc tccttcgcga g
//
LOCUS       DAOSOU010000013        18306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3052_length_18306_cov_5.785984, whole genome shotgun sequence.
ACCESSION   DAOSOU010000013 DAOSOU010000000
VERSION     DAOSOU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 18306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18306
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3052_length_18306_cov_5.785984"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLW15_01425"
     CDS             <1..612
                     /locus_tag="PLW15_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT33091.1"
                     /translation="SVAVDRALLRYDRQGDEHYDVISAFIKSVRGSDVDAALHYLARM
                     IEAGEDPRFIARRIIISAAEDVGIADPQALLVAMAAADAVQLIGMPEGRIPLAEAVVY
                     LATAPKSNAAYMALDAAIADVRAGRIGRVPKHLRDAHYPGAKRLGHGKGYQYSHDSEI
                     GVVAQQYLPDELVGTEYYHPSGHGNERDVAARLEKLRAIIRGG"
     gene            804..>1423
                     /gene="rpsD"
                     /locus_tag="PLW15_01430"
     CDS             804..>1423
                     /gene="rpsD"
                     /locus_tag="PLW15_01430"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOT33092.1"
                     /translation="MSTKSRTRSKTRLSRALGIALTPKAAKYLEKRPYAPGEHGRTKR
                     KQDSDYAVRLREKQRLRAQYGIREAQLKIQFEEARKAAGLTGENLVEQLEMRLDAILV
                     RSAIARTTAQARQMVVHRHILVDGQLVDRPSFRVKPGQLVHVKPRSEGMDIFQVAAAG
                     GHVDVLPKTPGYLEVELDKLHARLVRRPKRAEVPVTCDVQLVVEYYA"
     assembly_gap    1424..1523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1524..1745
                     /locus_tag="PLW15_01435"
     CDS             <1524..1745
                     /locus_tag="PLW15_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HOT33093.1"
                     /translation="EATTTISETNKQLVRVDSITSSVEEVTGNVSALVALFAATVGGP
                     LIKIAGFSAGVRAAIVGTRSARKSVPPTK"
     gene            1785..2012
                     /locus_tag="PLW15_01440"
     CDS             1785..2012
                     /locus_tag="PLW15_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33094.1"
                     /translation="MKSIILLAVGVAAGFIVAHQVAKTPEGQRFFEDVDTKAREFGSA
                     VIDGYKAREAELRAAVAEAEDVIADLSKRVK"
     gene            2092..4746
                     /gene="alaS"
                     /locus_tag="PLW15_01445"
     CDS             2092..4746
                     /gene="alaS"
                     /locus_tag="PLW15_01445"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT33095.1"
                     /translation="MQTADIQNRWLNFFGDRGHTIVPSASLVSDDPTLLFTVAGMVPF
                     VPYLTGLVPPPYPRATSVQKCIRTLDIEEVGKTPRHGTFFQMNGNFSFGDYFKEQAIE
                     YAWELLTSNESDGGLGFDEKDLWVTVYKDDDDAVNFWKKTAGLPDERIQRLDMDTNYW
                     STGQPGPAGPCSEIFFDRGPAYGIDGGPATDDDRYVEIWNLVFMQYLRGEGAGKNFEI
                     LGELPKKNIDTGMGMERVAFIKQGVENMYEIDQVRPVLDKAAELSKRKYGANHDDDVR
                     MRVIADHVRSSLMLMSDGVAPSNEGRGYILRRLLRRSVRAMRLLGVEEPTFGELFPAS
                     RDAMKVQYPEVSADYDRISRLALAEEETFLRTLASGTSILDLAVTRTQQAGSTELPSD
                     TAFLLHDTYGFPIDLTLEIAEDAGLTVDRDAFDSLMLHQRTIAKADAKSKKQHLADLS
                     VYSAFRAQGETVFTGYDYLTTDSMILGLIVNGESVKSASAGDIAEVILAETSLYPESG
                     GQEADAGTIVGTGFELEVLDVQRPVKGLVSHKVQVTSGEVGVGDAATTVVDPEWRRGA
                     TQAHSATHLIHAALRQILGPEAHQSGSYNKAGYMRLDFSWNQALSHATKTEIEEVANN
                     AVRDNLEVQTRILPLDEAKALGAMALFGEKYGDTVRVVEIGGPWSLELCAGTHVTRSS
                     EIGLINLVGESSVGSTNRRIESLVGIEAFRDLATERAIVSQLTGTLKTPREHLPERIA
                     ELMANLKAAERKIAQYEAGQLAQRVPALVATAHPVGAVTVVAENVGEVATGDDLRSLV
                     TSVRERLGSAASVVALAADVAGKPAVIVATNDASRSAGVKAGALAKLAAGVLGGGGGG
                     KDDLAQGGGADVAAIPAALDAIVASLGA"
     gene            4749..5210
                     /gene="ruvX"
                     /locus_tag="PLW15_01450"
     CDS             4749..5210
                     /gene="ruvX"
                     /locus_tag="PLW15_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT33096.1"
                     /translation="MRPGVRLGIDVGTARIGVARSDLHGMLATPVETVARGAGDIARI
                     CELVSEIDAVEIIVGLPLALSGRETASTGDAKEFAGRLSTACEVPVRLVDERLSTVSA
                     NSALRASGRNAKNSRSVVDQVAAVIILQHALDFERSADSPPGAVVDPDEGL"
     gene            5213..7258
                     /gene="mltG"
                     /locus_tag="PLW15_01455"
     CDS             5213..7258
                     /gene="mltG"
                     /locus_tag="PLW15_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT33097.1"
                     /translation="MANEPSWDELFSPQPAQPEATRPYSIDPEPVSAAPVQSDDDPFA
                     ALFGGLTTGPSPVVPAQQEAPVAPAQQSAPPEFFVTAQQPVPTAFPAPPAQNAPFVNP
                     EPALSLPPVQDSLPVQQAAPPGWPVQPEAAPADPFAGLFGSAAAAPAPLVEPPAPAQS
                     ADAFAAVFAAPQEPSTLPPASQPAFAAPVAAAAVPTTEAAPLTRREMREREANGGIVA
                     AASASVIPASLTPAEVAPAAFTPAAAPVAAAVPSAAEPVDFFASLASPAPAAAVPPAA
                     TADDVFPFDFGGGSGGGSNSGSGGGFGGGSGGDGGDRQRPDQPKKRRNLLWLKITLPI
                     VIVLGLVGGGAAYAWLNYETQVREFLGWQLPNDYEGAGNGEEAIVTIQSGDLGSDVAN
                     TLKDAGVTMTFDAFYDLLLAQPDNVNFLPGNYALQKQMSAQAALDALLDPANKVTSKL
                     LITEGTTLPSALEIIAETTGIPLADVQAAAADPTVYGVPAGEPSLEGYLFPATYELDG
                     SETAQDILQLMVDTMFQHLDDAGVAPENRHTVLTLASIIQREAGSNQDDFYKIARVFQ
                     NRLDQGINLQSDATVAYGTGNLHTVWTTDDERADASNLYNTYANPGLPIGPIGLPGDL
                     AIQAALNPADGPWLFFVPINLATGETVFSETAEEHEAAVDQLHAWCDESEENASYCE"
     gene            7251..8084
                     /locus_tag="PLW15_01460"
     CDS             7251..8084
                     /locus_tag="PLW15_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT33098.1"
                     /translation="MSDAGSRLAVLGSPISHSKSPAIQRAAYGVLGLDWTYEAIEMTG
                     DDLGDFVAGLDDQWRGLSLTMPLKRDVLALLAWRHPLVDLVGGANTVLLSAEGPRGFN
                     TDVQGAVESFASVGVSSLGYVHLLGAGATAASVLVAVAQLGAARVLVSARSPERALGI
                     VELGGRLGVDVVVRPWGIMDRSMIVPDAVVSTVPGGQNDLVFAEPIRAGSVLFDVAYD
                     PWPSRLASDWMSAGGTVISGLDLLVNQAVGQVRLFVAGDQFAPLPNELEVIAAMRAAV
                     A"
     gene            8116..9321
                     /gene="aroC"
                     /locus_tag="PLW15_01465"
     CDS             8116..9321
                     /gene="aroC"
                     /locus_tag="PLW15_01465"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT33099.1"
                     /translation="MLRWLTAGESHGPELVAILEGLPAGVSVSPEFIQEHMHRRSLGY
                     GRGARMKFEQDELSISGGVRFGLTMGSPVALRIGNTEWPRWVEVMSAVPVDLDSLPKG
                     RGAALTRPRPGHADLVGMQKYDFDEARNVLERASARETAARVALGAVARAFLSELGIE
                     IISHTLAIGTVRVPDGAALPEPGDVAALDADPLRCFDAATSAEMVAEVDRAHDDGDTL
                     GGVVEVLAFGLPPGLGSYTHWDRRLDSQLAAAVMGIQAIKGVEIGDGFETARRRGSAA
                     HDELVMGADGITRLSDRAGGTEGGMSTGTLLRVRAAMKPISTVPHALRTVDVATGTAA
                     EAHHQRSDVCAVPAAGVVAEAMVALVLANSVLEKFGGDSVGETKRNLDAYLASIPENL
                     TTARASQSE"
     gene            9314..9820
                     /locus_tag="PLW15_01470"
     CDS             9314..9820
                     /locus_tag="PLW15_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT33100.1"
                     /translation="MSDEGAVVVLIGAPGAGKTRTGKRLARLLGVPFIDTDKRIVAAH
                     GPIATIFEDHGEPHFRSIERETVAEALRERAVVTLGGGAVLDPRTQADLAEKRVVQLT
                     VSAAAVEKRIATGKRPLLKDGIDAWTAIVTARQPIYDRLSQLSVDTSRQPLDRVAQRI
                     VNWLEKQD"
     gene            9821..10897
                     /gene="aroB"
                     /locus_tag="PLW15_01475"
     CDS             9821..10897
                     /gene="aroB"
                     /locus_tag="PLW15_01475"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOT33101.1"
                     /translation="MTETTVIPVTGTDPYDVIIGRGLLASLADQLGPRVAKVLIVHPP
                     TLGAKANALRESLVDRYEVLLAEVPDAEDAKRVEVAAFCWQIMGQTDFTRTDAVVGLG
                     GGATTDLAGFVAATWLRGVRLVQCPTSVAGMVDAAVGGKTGINTAEGKNLVGSFYAPA
                     AVVVDLDTLDTLPRNEILAGFAEVVKCGFIGEPEILDIIEADVDRATDPTSDEFRRVV
                     ELSIGLKARVVSEDFKESGLREILNYGHTLGHAIEHAERYRWRHGAAISVGMVFAAEL
                     GRIAGSLSDEVVDRHRRILESLTLPVDYPLGRWKTLLATMQRDKKARAGMMRFIVLDE
                     VGKTSVLAGPEEALLFAAYQEVGV"
     gene            10894..11532
                     /locus_tag="PLW15_01480"
     CDS             10894..11532
                     /locus_tag="PLW15_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOT33102.1"
                     /translation="MTIVQALGSFAVLAALLTIIPGLDTTLVLRSSLTQSRAHAYATA
                     FGICSGAFVWGAAAAVGAAALLAASEVAFTVLKLAGAAYLLYLGISMIVASFRHTAVP
                     EADTPARPTGRLSAAFGRGLLSNILNPKVGVFYIALIPQFIPQGVPPLGMGLLLALVH
                     VVESLIWFSAIIFASQLARRWFTNPKVVRWIDRVTGGVLVGFAGVLAVESTP"
     gene            11632..12093
                     /locus_tag="PLW15_01485"
     CDS             11632..12093
                     /locus_tag="PLW15_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase LigA"
                     /protein_id="HOT33103.1"
                     /translation="MTATPITISAPTSRLVRLVGILGIIAGVIMIVAGATVWVTVSSQ
                     LAAEKITVAEDAAFLAGAPVADPFTAYAEADIISHHALEASGGATYAELDREDPVRIT
                     VMNASFLRTSLFTSIVSFGVAAFVAGVGALFILFGWALTRLAPRGTVHSEP"
     gene            12090..12527
                     /locus_tag="PLW15_01490"
     CDS             12090..12527
                     /locus_tag="PLW15_01490"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOT33104.1"
                     /translation="MTTVLVLNGPNLGRLGSREPDVYGSGTLDDLRGLLEGEASGLTI
                     DLRQTDDEATLIGWLHEAVDSGSPVILNPAAFTHYSYALRDAAALVTKAGLLLVEVHI
                     SNPHAREEFRHTSVISAVATGVIAGFGFESYLLALDLVARRLG"
     gene            12605..13168
                     /gene="efp"
                     /locus_tag="PLW15_01495"
     CDS             12605..13168
                     /gene="efp"
                     /locus_tag="PLW15_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT33105.1"
                     /translation="MASTADIRNGVVLNMDGGLWTVIDFQHVKPGKGGAFVRTKVKNV
                     MTGKVVDRTFNAGAKIETETVDRADFQYSYKDGDNFVFMDLGNYDQITLTPAQVGDAA
                     NFMLENQNVTIALHNGDPLYVELPASVVLEITYTEPGLQGDRSTGGTKPATVETGYQI
                     QVPLFLEQGTKVKVDTRDGSYLGRVTD"
     gene            13200..13613
                     /gene="nusB"
                     /locus_tag="PLW15_01500"
     CDS             13200..13613
                     /gene="nusB"
                     /locus_tag="PLW15_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT33106.1"
                     /translation="MGARSKSRKRALDILYSADVRGESINAALADEAARNAADPKRSN
                     SWEYAREIVVGITEHGDEIDELIETYAQGWTISRMPAIDRALLRIGIWEILFNDGVPD
                     SVAIAEAVESARVHSTEDSAGFVNGLLGRIAATRA"
     gene            complement(13610..14569)
                     /gene="ppk2"
                     /locus_tag="PLW15_01505"
     CDS             complement(13610..14569)
                     /gene="ppk2"
                     /locus_tag="PLW15_01505"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOT33107.1"
                     /translation="MSKDAADSAHREFPAADPGQVDVTPEIDELEELEELLGDASPTA
                     EADRRAWRQGYPYKAKMTRRDYENQKRRLQIELLKLQAWTKESGQKVVILFEGRDAAG
                     KGGAIKRFMEHLNPRGARVVALEIPTDRERSQWYFQRYVQHLPTSGEIVMFDRSWYNR
                     AGVERVMGYCSPHEYLEFTRSAPEFERMLVQSGIHLIKFWFSVGRAEQRARFTARSHD
                     VVKQWKLSPTDLASLDKWDDYTRAKEAMFFYTDTPLAPWTVVKSNDKKRARIEAMRWV
                     LSLFDYAGKDREVVAVPDSRIIGSPATVYDEGETPGGAFPVVG"
     gene            14714..15241
                     /gene="pyrR"
                     /locus_tag="PLW15_01510"
     CDS             14714..15241
                     /gene="pyrR"
                     /locus_tag="PLW15_01510"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT33108.1"
                     /translation="MTARSVLQQADIARALTRISHEILESNRGSSDLVLLGIPTRGVI
                     LARRIAGILDSIEPGSGTVGSLDVTMYRDDLAKNPTRAPSPTDIPAGGIDGKTVILVD
                     DVLYSGRTIRAALDALSDLGRPRAVRLAALVDRGHRELPIRADFVGKNLPTSTDERIN
                     VRLMETDGEEGVTIQ"
     gene            15238..16182
                     /locus_tag="PLW15_01515"
     CDS             15238..16182
                     /locus_tag="PLW15_01515"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOT33109.1"
                     /translation="MRHLLSTRDLRRDEVVALLDIAEDMADVSGREIKKLPALRGKTV
                     VNLFFEDSTRTRVSFEVAAKRLSADVINFSAKGSSVSKGESLKDTAQTLAAMGADAVV
                     IRHGASGAPRVLADSGWIDAGIVNAGDGTHEHPTQALLDAFTIRKRLHGVASRGRGLD
                     GVAVTIVGDILHSRVARSNVWLLTTLGAHVSLVAPATLLPVDTSHWPVTVGYDLDIAI
                     DSAPDVVMMLRIQSERMNAAFFPNAREYSREWGLGDDRVDRLPAASMVMHPGPMNRGL
                     EISAAAADSVQSTVLEQVANGVSIRMAVLYSLLAGSDS"
     gene            16179..17486
                     /locus_tag="PLW15_01520"
     CDS             16179..17486
                     /locus_tag="PLW15_01520"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT33110.1"
                     /translation="MSIHVKGAVLPDGSNRDLFIDNGTFVEALEKTPTTVIDASGLLA
                     LPGLVDLHTHLREPGFEQSETVLTGSRAAAAGGYTAVFAMANTMPVQDTAGVVEQVQS
                     LGERHGYVTVRPIGAVTAGLAGERLAEIGAMAQSRAQVRVFSDDGHCVHDALLMRRAL
                     EYVKTFGGVVAQHAQEPRLTVGAQLNEGALSGELGLVGWPAVAEESIIARDVLLAQHV
                     GARLHVCHISTAGSVEVIRWAKARGVDVTAEVTPHHLLLTEELARGYDARFKVNPPLR
                     RDEDVHALRAALADGTIDIVATDHAPHPLEAKDCAWDEAANGMVGLESALSVVHAAVV
                     ESGQLNWSDVARVMSSRPAEIGLLDGYARPFAVGSAANLALYDTAATRTFTVEDLRGK
                     SVNSPYLGRTLPGRVVATVHRGQPTVLDGELVPADLVAAGDRG"
     gene            17497..17967
                     /locus_tag="PLW15_01525"
     CDS             17497..17967
                     /locus_tag="PLW15_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33111.1"
                     /translation="MAIVVAFMLVLLGLLAVGWYARRRRQSAIATPATVPSDPGTFVG
                     QFAGKYVATTVSGDPFDRIAVHGLGFRGSVEVTVTVAGLVVRIVGENGIWIPREALRG
                     IRRASWTIDRAVETDGLHLIEWSLGNSAVDSYFRMDEPTGFGTAVDSIIERQTA"
     gene            17964..>18306
                     /locus_tag="PLW15_01530"
     CDS             17964..>18306
                     /locus_tag="PLW15_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HOT33112.1"
                     /translation="MTLEPAVLVLEDGTRYPGHAYGARGRTLGEVVFATGMTGYQETL
                     TDPSYAGQIVVMTAPHIGNTGVNDEDPESSRIWVSGYVVRDPSRVVSNHRATGALDDE
                     LVSEGVVGISGI"
BASE COUNT         2994 a         6142 c         6019 g         3051 t
ORIGIN      
        1 tcggtggcgg tcgaccgcgc cctgctgcgc tacgaccggc agggcgacga gcactacgac
       61 gtgatcagcg cgttcatcaa gtcggtacgc ggctcggacg tcgatgcggc tctgcactat
      121 ctggcgcgca tgatcgaggc gggggaggac ccgcgtttca tcgcacggcg catcatcatc
      181 tcggcggctg aggacgtcgg catcgccgac ccacaggcct tgctggtcgc gatggctgcc
      241 gccgatgccg tgcagctcat cggcatgccg gagggacgca tccctcttgc cgaggccgtc
      301 gtgtacctcg cgacagcgcc gaagtcgaac gccgcctaca tggctctgga tgccgcgatc
      361 gccgacgttc gcgccggccg catcggtcgc gtacccaagc acctccgcga cgcccactac
      421 ccgggggcga aacgcctcgg gcacggcaag ggctaccagt actcccacga ctccgaaatc
      481 ggcgtggtcg cgcagcagta tcttcccgac gaactcgtgg gcaccgagta ctaccacccg
      541 agcgggcacg gcaatgagcg ggacgtagcg gcccggttgg agaagctgcg cgcgataatc
      601 cgcggcggat gatagtattt gacccgtcca aagcctgatt tcctgagcgc ggctgcaacg
      661 gaatcatcgg ctgaggagca tgcggtctgt agccgaccga gctccacgga acatccctct
      721 gattttcacc ggattgttgt tgcgcgccca cgcgtgtgcg cgaaccaccg gttgccccgc
      781 aagaactcac agaaaaggac accgtgtcta ccaagtcacg cactcgtagc aagacccgcc
      841 tctcgcgcgc gctgggcatc gccctcaccc cgaaggccgc caagtacctc gagaagcgcc
      901 cgtacgctcc cggcgagcac ggccgcacca agcgcaagca ggacagcgac tacgccgtcc
      961 gtctgcgcga gaagcagcgc ctgcgtgccc agtacggcat ccgtgaggct cagctgaaga
     1021 ttcagttcga ggaggcccgc aaggccgccg gcctgaccgg tgagaacctg gttgagcagc
     1081 tcgagatgcg tctcgacgcg atcctcgtgc gctccgcgat cgctcgcacc accgctcagg
     1141 cccgccagat ggtcgtgcac cgccacatcc tcgtcgacgg ccagctcgtc gaccgtccgt
     1201 cgttccgcgt caagcccggc cagctcgttc acgtgaagcc gcgcagcgag ggcatggaca
     1261 tcttccaggt cgccgctgcc ggtggccacg tcgacgttct gccgaagacc ccgggctacc
     1321 tcgaggtcga gctcgacaag ctgcacgctc gtctcgttcg ccgcccgaag cgcgccgagg
     1381 tccccgtgac ctgtgacgtg cagctggtcg tcgagtacta cgcnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnngaggcga cgacgaccat cagcgagacc aacaagcagt
     1561 tggtgcgtgt ggacagcatc acctcgagcg tcgaagaggt gacgggcaac gtctccgccc
     1621 tggttgccct cttcgctgcg acagtcggtg ggccactcat caagatcgcc ggcttcagcg
     1681 ccggcgtcag ggcggccatc gtgggcaccc gctccgcccg gaagtcagtg ccgccgacga
     1741 agtaagcttg atccgtcgtc gtccgtattc cgaaggagct tgtcatgaag agcatcatcc
     1801 tgctggccgt cggtgttgcc gccggtttca tcgtcgcgca ccaggtcgcc aagacgcccg
     1861 agggccagag gttcttcgaa gacgttgaca ccaaggcacg tgagttcggc agcgccgtca
     1921 tcgatggcta caaggcccgc gaggcggagc tgagggcggc cgtcgccgag gccgaggacg
     1981 tcatcgccga cctctccaag cgcgtcaagt aggtctcgga gccgtacgcg gccgatcgac
     2041 caccgggagt tcccgggcgc atccacattt cgacccggga ctttcacaca catgcagacc
     2101 gcagacatcc agaaccgttg gctgaacttc ttcggcgacc gggggcacac catcgtgccg
     2161 tcggcatcgc tggtcagcga cgacccgacg ctgctgttca ccgtggccgg catggtgcca
     2221 ttcgttccgt acctgaccgg tctggtgccg cccccgtatc cccgcgcgac gagcgtgcag
     2281 aagtgcatcc gcaccctcga catcgaggag gtcggcaaga ctccgcgcca cggaacgttc
     2341 ttccagatga acggcaactt ctccttcggc gactacttca aggagcaggc gatcgagtac
     2401 gcctgggagc tgctcaccag caatgagagc gatggcggtc tgggcttcga cgagaaggac
     2461 ctgtgggtca ccgtctacaa ggacgatgac gatgccgtga acttctggaa gaagacggct
     2521 gggctccccg acgagcgcat ccagcgactc gacatggaca cgaactactg gtcgaccggg
     2581 cagccgggac cggccggccc ctgctcggag atcttcttcg accgcggtcc ggcctacggc
     2641 atcgatgggg gaccggcgac cgacgacgac cgctacgtcg agatctggaa cctcgtcttc
     2701 atgcagtacc tgcgcggcga gggtgcaggc aagaatttcg agatcctcgg cgagctgccg
     2761 aagaagaaca tcgacaccgg catgggcatg gagcgcgtcg ccttcatcaa gcagggcgtc
     2821 gagaacatgt acgagatcga ccaggtgcga ccggtgctcg acaaggctgc agagctgtcg
     2881 aagcgcaagt acggcgccaa ccacgatgac gacgtgcgga tgcgagtgat cgccgaccac
     2941 gtgcgctcga gcctcatgct gatgtccgac ggagttgcgc cgagcaacga gggtcgcggc
     3001 tacatcctgc gtcgcctgct gcgtcgcagc gtgcgggcca tgcgtcttct cggggtggag
     3061 gagccgacgt tcggcgagct gttccccgcg tcgcgcgacg cgatgaaggt gcagtacccc
     3121 gaggtgtccg ccgactacga tcggatctcg cgcctggccc tcgcggagga ggagaccttc
     3181 ctgcgcacgc tggccagcgg cacgagcatc ctcgatctgg cggtcacgcg cacccagcag
     3241 gcgggctcga ccgagctgcc gagcgacacg gcgttcctcc tgcacgacac gtatggcttc
     3301 ccgatcgacc tcacgttgga gatcgccgaa gacgccggat tgacggtcga tcgcgacgcc
     3361 ttcgattccc tcatgctgca ccagcgcacc atcgccaagg ccgacgcgaa gtcgaagaag
     3421 cagcacctcg ccgacctgtc ggtctacagc gcgttccgcg cccagggcga gacggtcttc
     3481 acgggctacg actacctgac gaccgacagc atgattctcg ggctcatcgt caatggcgag
     3541 tcggtaaaga gcgcttcagc cggtgacata gccgaagtga tccttgccga gacctcgctc
     3601 tacccggagt ccggcggcca ggaggccgac gccggcacca ttgtgggcac gggcttcgag
     3661 ctcgaggtgc tcgacgtgca gcgacccgtc aagggcctcg tgagccacaa ggtgcaggtg
     3721 acctcgggtg aggtcggggt cggggatgca gcgaccacgg tggtggaccc ggagtggcgt
     3781 cgtggcgcga cgcaggcaca ctccgcgacc cacctcatcc acgcggctct tcggcagatt
     3841 ctcggacccg aggcgcacca gtccggctcg tacaacaagg ccggctacat gcgactcgac
     3901 ttctcctgga accaggcgct gtcgcacgcg accaagaccg agatcgagga ggtcgcgaac
     3961 aacgccgtgc gcgacaacct cgaggtgcag acgcgaatcc tgccgctcga tgaggcgaag
     4021 gcactcggcg ccatggcgct gttcggtgag aagtacggtg acaccgtgcg ggtcgtcgag
     4081 atcggtggcc cgtggtcact cgagctgtgc gcgggaactc acgtgacgcg ctcgtcggag
     4141 atcgggctca tcaatctggt gggggagtcg agcgtcggct cgaccaaccg acgcatcgag
     4201 tcgctggtcg gtatcgaggc gttccgcgat ctggcaaccg agagggcgat cgtctcgcag
     4261 ctgaccggca cgctgaagac cccgcgggag caccttcccg agcgcatcgc cgagttgatg
     4321 gcgaatctca aggcagccga acgcaagatc gctcaatacg aggcaggtca gctcgcacag
     4381 cgggtgcccg cactcgtcgc gacagctcat ccggttggcg cagtgacggt tgttgccgag
     4441 aacgtgggcg aagtggccac cggcgacgac ctgcgaagcc tggtgaccag cgttcgcgag
     4501 cggctcggtt cggcagcgtc ggtcgtggca ctcgcagcgg atgtcgcggg aaagccggcc
     4561 gtcatcgtcg cgaccaacga tgcgtcgcgc tctgccggag tgaaggctgg agcgctggcg
     4621 aagctggccg ccggagtact cggtggtggc ggtggcggca aggatgacct cgcccagggc
     4681 ggtggtgcgg atgtcgcggc tattcccgcc gcgctcgatg ccatcgtcgc gtccctcggg
     4741 gcctaggcat gcggccgggc gttcgcctcg gcatcgacgt ggggaccgcc cgcatcggtg
     4801 ttgcccgcag cgacctccac ggcatgctcg cgaccccggt cgagactgtg gccagaggcg
     4861 ctggtgacat cgcgcgaatc tgtgaactcg tgagcgagat cgacgcggtg gagatcatcg
     4921 taggtctgcc tcttgcgctg tccggccgcg agacagcgtc gacgggagat gccaaggagt
     4981 tcgccggacg gctgtccacg gcctgcgagg taccggtgcg cctggtggat gagcgcctat
     5041 cgacggtgtc tgccaattca gcgttgcggg catccgggcg aaacgcgaag aactcgcgtt
     5101 cggtggttga tcaggttgcc gccgttataa ttttgcaaca cgccctcgac ttcgagcgct
     5161 cggccgactc cccgcccggt gccgtcgttg acccggacga aggactgtga gaatggccaa
     5221 cgaacccagc tgggacgagc tgttctctcc gcagcccgct cagcccgagg ccacgcgacc
     5281 gtactcgatc gaccctgaac ctgtgagcgc cgcacctgtg cagagcgacg acgacccgtt
     5341 cgctgcgctc ttcggcggtc tcaccacggg tcccagtccc gtcgtgcctg cgcagcagga
     5401 ggcgcccgtt gcgcccgcac agcagagtgc tccgcccgag ttcttcgtga ccgcacagca
     5461 gcccgttccc accgccttcc ctgcacctcc ggcgcagaac gcgccgtttg tgaacccgga
     5521 gccggcgctg agcctgccac ccgtgcagga ctcgcttccc gtgcagcagg cagcgcctcc
     5581 aggctggcct gtgcagccgg aagcggctcc tgcagatccg ttcgccggtc tgttcggctc
     5641 cgcggccgct gcgcccgcac ctctcgtcga accaccggca cccgcccaga gtgctgatgc
     5701 cttcgctgcc gtattcgccg caccgcagga gccgtccacg cttcctccgg cgtctcagcc
     5761 cgcattcgcg gcacccgtag ctgcggcggc ggtcccgacg accgaggcgg cgcctctcac
     5821 ccgacgcgaa atgcgcgaac gggaggccaa tggcggcatc gtggccgcgg catccgcaag
     5881 cgtcatccct gccagcctca cacccgcgga agtggcgccc gcggccttca cgcccgcggc
     5941 cgctccggtc gccgcagcgg tcccctcggc cgccgagccc gtggacttct tcgcttccct
     6001 ggcgagccct gcccccgcgg cagccgtgcc cccggctgcg acggccgacg acgtcttccc
     6061 cttcgacttc ggaggcggca gcggcggcgg ctcgaactcg ggttccggtg gcggtttcgg
     6121 tggcgggtcg ggcggcgacg gtggagatcg tcagcgtccc gatcagccga agaagcgtcg
     6181 caacctgttg tggctgaaga tcacgctccc gatcgtcatc gtgctcggcc tggtcggtgg
     6241 gggcgcggcg tacgcctggc tcaactacga gacccaggtg cgcgagttcc tggggtggca
     6301 gctgcccaac gactacgagg gtgccggcaa tggcgaggag gcgatcgtca ccatccagtc
     6361 gggcgatctc ggttccgacg tcgcgaacac tctgaaggat gccggcgtca caatgacgtt
     6421 cgatgccttc tacgacctgc tgctcgcgca gccggacaac gtcaacttcc tgcccggcaa
     6481 ctatgcgctt cagaagcaga tgagcgcgca ggccgcgctt gacgcgcttc tggatcccgc
     6541 caacaaggtc acctcgaaac tgctcatcac cgagggcacc acgttgccca gcgcgctcga
     6601 gatcatcgcc gaaacgacgg gcattccgct cgccgacgtt caggcagcag ccgccgaccc
     6661 gactgtgtac ggtgttcccg ccggggagcc gagcctggag ggttacctgt tcccggccac
     6721 ctacgagctt gacggctccg agacggcgca ggacatcctc cagctcatgg tcgacaccat
     6781 gttccagcat ctggatgacg cgggcgtcgc gcccgagaac cgtcacaccg tgctcacact
     6841 cgcgtcgatc atccagcgtg aagccggctc caaccaggac gacttctaca agatcgctcg
     6901 cgtgttccag aaccgcctcg atcaggggat caacctgcag tcggatgcca cggtggcgta
     6961 cggcaccggg aacctccaca ccgtctggac gaccgacgac gagcgcgccg atgccagcaa
     7021 cctgtacaac acctacgcga accccggcct gccaatcggc ccgatcggac ttcctgggga
     7081 tctcgcgatc caggcggccc tgaacccggc ggacggcccg tggctgttct tcgtgcccat
     7141 caacctcgcc accggcgaga cggtcttctc cgagaccgcc gaagagcatg aggctgccgt
     7201 cgaccagctg cacgcgtggt gtgacgagag cgaagagaac gcttcctact gtgagtgacg
     7261 cgggctcgcg cctcgcggtg ctcggctcgc ccatctcgca ttcgaagtcg cctgcgatcc
     7321 agcgcgccgc ctacggtgtc ctcggtctcg actggaccta cgaagcgata gagatgaccg
     7381 gggacgacct cggcgacttc gttgccgggt tggatgacca gtggcgaggc ctgtctttga
     7441 cgatgccact caagcgcgac gtgctcgcgc tgctggcgtg gcggcatccg ctggtcgacc
     7501 tcgtcggcgg ggcgaatacg gtactgctct cggccgaggg gccgcgcggg ttcaacaccg
     7561 acgtgcaggg cgctgtcgag tcgttcgcca gcgtgggtgt gtcgagcctg ggatacgtgc
     7621 acctgctcgg tgcgggtgcg actgcggcat ccgtgctggt tgccgtcgcc cagctggggg
     7681 cagcccgggt gctggtgtcg gcgcgttctc cggagcgagc gctcggcatc gtcgagctcg
     7741 gcggccgact cggtgtcgat gtcgtcgttc ggccctgggg catcatggat cgctcgatga
     7801 tcgttccgga tgcggtggtc agcaccgttc ccggcggtca gaacgatctc gtgttcgccg
     7861 agcccattcg cgccggatcg gtgctctttg acgtcgccta cgatccgtgg ccgagtcgac
     7921 tcgcctccga ctggatgtcg gctggcggca ccgtgatctc aggactcgac ctgctcgtca
     7981 accaggccgt ggggcaggtg cggctcttcg tcgccgggga ccagttcgcc ccactcccga
     8041 acgagctcga ggtcattgcg gcgatgcggg cagccgtcgc ctgagcgcac cctcacgctg
     8101 tgggaagatc gacacatgct tcgttggttg acggctggtg agtcgcacgg tcccgagttg
     8161 gtcgccattc tggagggtct ccccgcggga gtatcggtga gcccggagtt catccaggag
     8221 cacatgcacc gacgctccct cggctacgga cgtggtgctc gaatgaagtt cgagcaggac
     8281 gagctgagca tcagtggcgg cgtgcgattc gggctcacca tgggaagccc ggttgctctg
     8341 cgcatcggca acaccgagtg gccacgctgg gtcgaggtca tgagtgcggt tccagtggac
     8401 ctcgacagcc ttccgaaggg tcgcggggcg gcgctcacca ggccacgtcc cggccacgcc
     8461 gatctggtcg gcatgcagaa gtacgacttc gacgaggcgc gcaacgtgct cgagcgcgcg
     8521 agcgcgcgcg agacggccgc gcgggtggcg ctcggggctg tagcacgcgc cttcctgtcg
     8581 gagctcggca tcgagatcat cagccacacg ctcgcgatcg gcacggtccg cgtgcccgat
     8641 ggtgcggcgc tgcccgagcc gggcgacgtc gccgcgctcg acgccgaccc gctgcgctgc
     8701 ttcgacgccg ctacatccgc cgagatggtc gccgaggtcg atcgggctca cgacgacggc
     8761 gacaccctcg gcggcgtggt cgaggtgctg gcgttcggac ttccgccggg gctcggctcg
     8821 tacacccact gggatcgccg cctcgactct cagctggcag ccgctgtgat gggtatccag
     8881 gcgatcaagg gtgtcgagat cggcgatgga ttcgagactg cgcgtcgccg gggatccgcc
     8941 gcccacgacg aactggtcat gggtgccgac ggcatcactc ggctctccga tcgtgccggt
     9001 ggcactgagg gcgggatgag cacgggaacg ctcctgcgcg tgcgagccgc catgaaaccc
     9061 atctcgaccg tgcctcacgc cctccgcacc gtcgacgtag cgacgggaac tgccgccgag
     9121 gcccaccacc agcgttccga cgtgtgcgcc gtgcctgcgg ctggtgtcgt cgctgaggcg
     9181 atggtcgcgc tcgtgctcgc caattcggtg ctggagaaat tcggcggcga ttcggtcggt
     9241 gagaccaagc gcaacctcga cgcctacctc gcctcgattc ccgagaacct gacgactgca
     9301 cgggcctcgc agagtgagtg acgaaggcgc ggtcgtcgtc ctcatcggtg cgccgggagc
     9361 cggcaagacc cgcacgggca agaggctcgc gcgcctgctg ggggtgccgt tcatcgacac
     9421 cgataagcga atcgtggccg cccatggacc gatcgcgaca atcttcgagg atcacggtga
     9481 gccgcatttc cgctccatcg agcgtgagac ggtggccgag gcgttgcggg agcgggccgt
     9541 ggtgaccctc ggcggtggcg ctgtcctcga tccccgtacg caggccgact tggccgagaa
     9601 gcgggtggtg cagttgacgg tgtccgccgc tgccgttgag aagcgaatcg cgaccggcaa
     9661 gcggccgctt ctgaaggacg gcatcgacgc gtggacggcg atcgtcaccg cgcgccaacc
     9721 gatttacgat cgactgtctc agctgagcgt cgatacctct cgccagccgc tggatcgggt
     9781 agcgcagcgc atcgtgaact ggctggagaa gcaggactaa atgaccgaga ccaccgtcat
     9841 ccccgtcacg ggcaccgacc cctacgacgt catcatcgga cgtgggcttc tcgcgtccct
     9901 tgccgaccag ctgggcccgc gagtggcgaa ggtgctgatc gtgcatccgc cgactctcgg
     9961 ggcgaaggcc aacgcgcttc gcgagtcgct ggtcgaccgc tacgaggtgc tcctcgcgga
    10021 ggtgcctgat gccgaggacg cgaagcgcgt cgaggtcgcc gccttctgtt ggcagatcat
    10081 gggccagacc gacttcacga gaacggatgc cgtggtcggc ctgggcgggg gagcgacgac
    10141 cgatctggcc ggcttcgtcg ccgccacgtg gctgcgcggg gtgcgcctgg tgcagtgtcc
    10201 gacgagcgtc gccggaatgg tggacgctgc cgtcggcggc aagaccggca tcaacacagc
    10261 ggagggcaag aacctcgtcg gatcgttcta cgctcccgca gctgtcgtcg tggacctcga
    10321 cactctcgac acgctcccgc gcaacgagat cctcgcaggc ttcgccgagg tcgtgaagtg
    10381 cggcttcatc ggcgaacccg agatcttgga catcatcgaa gcggatgtgg accgcgccac
    10441 cgaccccacc agcgacgagt tccgccgggt cgtcgagctg tcgatcggcc tgaaggcgcg
    10501 cgtcgtgagc gaggacttca aggagtccgg tctgcgcgag attctcaact acgggcacac
    10561 cctgggtcac gcgatcgagc acgccgagcg gtaccgctgg cggcacggtg cggcgatctc
    10621 ggtgggcatg gtgttcgcgg cggagctggg acgcatcgcg ggctcgctca gcgacgaggt
    10681 cgtggaccgg caccgtcgca tcctcgagtc gctgacgctg ccggtcgact acccgctggg
    10741 tcgatggaag acactgctgg cgaccatgca gcgcgacaag aaggcgcgcg cggggatgat
    10801 gcgcttcatc gtgctcgatg aggtcggcaa gacctcggtg ctggcgggtc ccgaggaggc
    10861 gctgctgttc gcggcgtatc aggaggtggg ggtgtgacga tcgtccaggc gctcgggtcg
    10921 ttcgccgtgc tcgcggcact gctcacgatc atcccgggcc tggacaccac gctggtgctg
    10981 cgctcgtccc tcacccagtc gcgcgcccac gcgtacgcga cggcgttcgg catctgctcg
    11041 ggggcattcg tctggggtgc cgccgcggca gtcggtgccg cggcgctgct cgctgcgtcg
    11101 gaggtcgcgt tcaccgtgct caagctcgct ggtgctgcgt acctgctgta cctcggcatc
    11161 tccatgatcg tcgcaagctt ccgccacacc gcggtgcccg aggcggatac cccggccagg
    11221 ccgacaggac gcctgtcggc ggccttcggc cgtggactgc tctccaacat cctcaacccc
    11281 aaggtcggcg tcttctacat cgcgctgatc ccgcagttca tcccgcaggg cgtgccgccg
    11341 ctggggatgg ggttgctgct cgcactcgtg cacgtcgtcg agtcgctgat ctggttctcg
    11401 gcgatcatct tcgcctcaca gctcgcgcgg cgttggttca ccaacccgaa ggtagtgcgg
    11461 tggatcgacc gtgtgaccgg aggagtcctc gtcgggttcg ccggtgtact cgctgtggag
    11521 tcgactccct gacctagggg accttcgacc ctgacgttct ctcgaatctg acgggagcat
    11581 gagtcagacg cgatcgcgtc atcgtgccag ttcgatcagg gagcagattt catgaccgcc
    11641 acacctatca ccatcagcgc gcccacctca cgacttgtcc gtctcgtggg gattctcggg
    11701 atcattgcag gagtcatcat gatcgtcgcc ggagcgacgg tatgggtcac cgtcagcagc
    11761 cagctcgccg ccgagaagat caccgtcgcc gaggatgccg cgttcctggc gggcgccccg
    11821 gttgctgacc cgttcacggc ctacgccgaa gccgacatca tcagccacca cgcgcttgag
    11881 gccagtggcg gcgccaccta cgccgagctc gatcgcgagg accccgtgcg gatcaccgtg
    11941 atgaacgcgt cgttcctgcg cacctcgttg ttcacgtcca tcgtctcgtt cggggtggcg
    12001 gcgttcgtgg cgggtgttgg cgcactcttc atcctcttcg gatgggccct cacgcgtctg
    12061 gcgccccggg gaacagtaca ctcggagccg tgaccaccgt cctcgtcctc aacggcccga
    12121 acctcggccg tctcggtagt cgtgagccgg acgtgtacgg tagcggcact ctagacgacc
    12181 tgcgcgggct gcttgagggc gaggcatccg gtctcaccat cgacctgcgc cagaccgacg
    12241 acgaggcgac tctcattggc tggctgcacg aggcagtcga tagcggcagc ccggtgattc
    12301 tcaaccccgc cgccttcacg cactattcct acgcgcttcg ggatgccgcg gcactcgtca
    12361 ccaaggcggg tctgttgctc gtcgaggtgc acatctccaa tccgcacgcg cgcgaggagt
    12421 tccgccacac cagcgtgatc tcggcggtgg cgaccggcgt gatcgccgga ttcggcttcg
    12481 agagctacct gctcgctctt gacctcgtcg cgcgccgcct cgggtagact cgcatgtcgg
    12541 tttcgatacg gacccttccg ggtcccactc aaccagcgtc ttcaacgaac ggataacttc
    12601 acccatggct tcaaccgctg atattcgcaa cggcgtggta ctcaacatgg acggtggcct
    12661 ctggacggtc atcgacttcc agcacgtcaa gcccggcaag ggtggtgcct tcgtgcgcac
    12721 caaggtcaag aatgtgatga cgggcaaggt cgtcgaccgc acgttcaacg ccggcgcgaa
    12781 gatcgagacc gagacagtcg accgcgccga cttccagtac tcgtacaagg acggcgacaa
    12841 cttcgtgttc atggatctgg gcaactacga ccagatcacc ctcaccccgg cgcaggtcgg
    12901 cgacgccgcc aacttcatgc tggagaacca gaacgtcacg atcgctctgc acaacggtga
    12961 cccgctctac gtcgagctgc cggcatccgt cgtcctggag atcacctaca ccgagcccgg
    13021 cctgcagggc gaccgctcga ccggcggcac caagcccgcg accgtggaga ccggctacca
    13081 gatccaggtg ccgctcttcc tcgagcaggg cacgaaggtc aaggtcgaca cccgcgacgg
    13141 cagctacctg ggccgcgtca ccgactgatg tcggtgacca acgaacagag aactgagtag
    13201 tgggcgcccg cagcaagtcc cgcaagcgcg ccctcgacat cctctacagc gccgatgtgc
    13261 gcggggagtc gatcaacgcg gcgctggccg atgaggcggc gcgcaatgcc gcagacccga
    13321 agcgcagcaa ttcgtgggag tacgcccgcg agatcgttgt cggcatcacc gagcacggcg
    13381 acgagatcga cgaactcatc gagacctacg cccaaggctg gacgatcagc cggatgcccg
    13441 cgatcgaccg cgcgctcctg cgcatcggga tctgggagat cctgttcaat gacggcgtgc
    13501 ccgacagcgt ggcaatcgcc gaggccgtcg agtccgctcg cgtgcacagc accgaggact
    13561 cagcgggctt cgtgaacggg ctactggggc gtatcgcggc gacccgcgcc taacccacga
    13621 ccgggaaggc accgccgggc gtctcgccct cgtcgtacac cgttgctgga ctgccgatga
    13681 tccgcgagtc cggcaccgcg accacctcgc ggtccttgcc ggcgtagtcg aacaggctca
    13741 gcacccagcg catcgcctcg atgcgcgcgc gcttcttgtc gttggacttg acgaccgtcc
    13801 agggcgccag gggtgtgtcg gtgtagaaga acattgcctc cttcgccctg gtgtagtcat
    13861 cccacttatc gaggctcgcg aggtcggtgg gggagagctt ccactgtttg acgacgtcgt
    13921 gcgagcgcgc tgtgaaccga gcgcgctgct cggcgcgccc gacggagaac cagaacttga
    13981 tgagatggat gccgctctgc acgagcatcc gctcgaactc aggtgccgac cgcgtgaact
    14041 cgaggtattc gtggggcgag cagtaaccca tcacgcgctc gacgcccgcg cggttgtacc
    14101 aggaacggtc gaacatcacg atctcgccgc tggtgggcag gtgctgcacg tagcgctgga
    14161 agtaccactg gctgcgctcc cgatcagtcg ggatctcgag cgcaacgacg cgtgccccgc
    14221 gggggttcaa atgctccatg aagcgcttga tcgcgccacc cttgcccgcg gcatcccggc
    14281 cctcgaagag gatgacgacc ttctggcccg actccttcgt ccacgcctgc agcttgagca
    14341 gctcgatttg caggcggcgc ttctggttct cgtagtcgcg gcgggtcatc ttcgccttgt
    14401 acgggtagcc ctggcgccac gcccgtcgat cggcctcagc ggtcggagac gcgtccccga
    14461 gcagttcctc gagttcctcg agttcatcga tctcgggggt cacatcgacc tgacccgggt
    14521 cggcggcggg gaactcccgg tgcgcggaat cggcggcatc cttcgacatg ccccgatggt
    14581 atccagaccc gctcgccctc ccgagaggct aaagtcccgt ccatcctcat cgggtgtgcc
    14641 cgcgctggcc gctatagtgg gctcaacaga tttcctttaa gttccgtcct gtgaggcggg
    14701 gaaggaggtc agaatgactg cacgctccgt gctgcagcag gctgatatcg cgcgtgcgtt
    14761 gactcggatt tcccacgaga tcctggagtc caatcgcggt tcatccgatc tcgttctcct
    14821 cggaatcccg actcgcggag tcattcttgc gcggcgcatc gcagggatac tggactccat
    14881 cgagcccggc tccggcaccg tggggtcgct cgacgtgacg atgtaccggg acgaccttgc
    14941 caagaatccg acccgcgcac cctcgccgac cgacatcccg gctggcggta tcgacggcaa
    15001 gaccgtgatt ctcgtcgacg atgtgctcta ctcgggccgc accatccggg ccgcacttga
    15061 tgccctcagt gacctgggtc gtccgcgtgc ggtgcgcctg gcggcactcg tcgatcgcgg
    15121 ccatcgggag ctgccgatcc gcgcagactt cgtgggcaag aacctgccga cctcaacaga
    15181 tgagcgcatc aacgtgcggc tcatggagac cgacggcgag gagggggtga cgatccagtg
    15241 aggcatctgc tctccacccg tgacctgcgc cgagacgagg tcgtcgcgct gctggacatc
    15301 gccgaagaca tggcggatgt ctccggccgc gagatcaaga agctgccggc gttgcggggc
    15361 aagacggtcg tcaacctgtt cttcgaggac tcgacccgca cccgcgtctc gttcgaagtc
    15421 gcggcgaagc gattgagtgc cgacgtcatc aacttcagcg ccaagggctc gagcgtctcc
    15481 aagggcgaga gcctgaagga caccgcccag acactcgcgg ccatgggggc ggacgccgtc
    15541 gtcatccgcc acggggccag cggcgcgccg cgcgtgctcg ccgacagcgg ctggatcgat
    15601 gcgggcatcg tcaatgcggg tgacggcact cacgagcatc cgacccaggc gcttctcgac
    15661 gcgttcacga ttcgcaagcg actgcacggg gttgcctcgc gaggccgcgg tctcgacgga
    15721 gtcgcggtga cgatcgtggg agacatcctg cactcgcgcg ttgctcggtc gaacgtctgg
    15781 ctgctcacga cgctgggcgc ccacgtctcg ctcgtcgcgc cggcgacact gctgccggtc
    15841 gacacaagtc actggcccgt caccgtcggc tacgacctcg acatcgcgat cgattcagct
    15901 cccgatgtgg tcatgatgct gcgcattcaa agcgaaagga tgaatgccgc cttcttcccc
    15961 aacgcccggg agtattcacg ggagtggggg ctcggcgacg atcgggtcga tcggctgcca
    16021 gcggctagca tggtgatgca ccccgggccg atgaaccgcg gccttgagat ctccgccgct
    16081 gctgccgact ccgtgcagtc gaccgtgctc gagcaggtcg ccaacggggt ctcgataagg
    16141 atggctgtgc tgtactcgct gctcgctgga agtgactcat gagcatccac gtgaagggtg
    16201 ctgtgctgcc cgacggaagc aaccgcgacc tgttcatcga caacggtacg ttcgttgagg
    16261 ctctcgagaa gacgccgacg acagtgatcg acgcctccgg gctgctcgcg ctacccggtc
    16321 tcgtcgacct gcacacccac ctgcgcgagc ccggcttcga gcagagcgag acggtgctca
    16381 ccggctcacg tgcagccgcg gcgggtggat acacggccgt gttcgcgatg gccaacacga
    16441 tgcccgtgca ggacaccgcc ggtgtcgtcg agcaggtgca gtccctcggg gagcggcacg
    16501 gctacgtgac tgttcggccc atcggggcgg tgacggctgg cctcgcgggg gagcggctcg
    16561 cggagatcgg cgccatggct cagtcccgcg cgcaggtgcg ggtgttctcc gatgacgggc
    16621 actgtgtgca cgacgccctg ctgatgcgcc gggccctcga gtacgtgaag accttcggcg
    16681 gagtagttgc ccagcatgcc caggagcccc gcctgaccgt gggtgcccag ctcaacgagg
    16741 gcgccctctc cggtgaactc ggcctggtcg ggtggccggc cgtcgccgag gagtcgatca
    16801 ttgcccgcga cgtgctcctc gcccagcacg tcggcgccag acttcacgtc tgccacatct
    16861 cgacagcggg ctcggtggag gtcatccgct gggccaaggc gcgcggtgtg gatgtcacgg
    16921 ccgaggtcac cccgcaccac ctgctgctca ccgaagaact cgcccgcggc tacgatgcgc
    16981 ggttcaaggt caatccgccg ctgcgccggg acgaggacgt tcacgccctg cgggccgccc
    17041 tcgccgacgg aaccatcgac attgtcgcaa ccgaccacgc accccaccct cttgaggcca
    17101 aggactgcgc gtgggatgaa gcggccaacg gcatggttgg cctcgagtca gcactgagtg
    17161 tcgtacacgc ggcggttgtc gagagcggcc agttgaactg gtcggatgtc gcgcgagtga
    17221 tgtcatcgcg tccggccgag atcggcctgc tcgacggata cgcccgaccg ttcgcggtcg
    17281 ggtctgccgc gaacctggcg ctgtacgaca ccgcggcgac ccgcaccttc acggttgagg
    17341 atctccgggg caagagcgtg aactcgccct acctgggccg cacgttgccg gggcgcgttg
    17401 tcgcgaccgt gcaccgcggc cagcccacag tgctcgacgg cgagctcgtg cccgccgatc
    17461 ttgtggcggc gggcgatcgt ggataggggc gtgctcatgg cgatcgttgt cgccttcatg
    17521 ctcgtgcttc tcggcctcct cgccgtgggc tggtacgcga ggcgccggcg ccaatccgcc
    17581 attgccacgc cggcgaccgt cccgtccgat cctggtacct tcgtcgggca gttcgctggc
    17641 aagtacgtcg ccacgaccgt gtcgggcgat ccgttcgacc ggatcgcggt gcacggtctc
    17701 gggttccgcg gatcggtcga ggtcaccgtg accgtcgccg gtctggtggt acggatcgtg
    17761 ggggagaacg gcatctggat tccccgggag gccctgcgag gcatccgcag agccagctgg
    17821 accatcgatc gggcggtcga gactgatggc ctgcacctca tcgagtggtc gctcggcaac
    17881 agtgccgttg acagctactt ccgcatggac gagccgaccg gcttcggcac ggccgtcgac
    17941 agcatcatcg aaaggcagac cgcgtgacgc tcgaacccgc cgtgctcgtc ctggaggacg
    18001 ggacccgcta ccccggccac gcctacggcg cccgcggccg cacccttggc gaagtggtct
    18061 tcgcgaccgg catgaccggc taccaggaga ccctcaccga ccccagctac gccgggcaga
    18121 tcgtcgtgat gacggcaccg cacatcggca acacgggcgt gaacgacgag gacccggagt
    18181 ccagccgcat ctgggtatcc ggctatgtcg tgcgggatcc cagtcgcgtc gtctccaacc
    18241 accgcgcgac cggggcgctc gacgacgaac tggtcagcga aggtgtggtg ggcatcagtg
    18301 gcatcg
//
LOCUS       DAOSOU010000014        18292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3056_length_18292_cov_6.330811, whole genome shotgun sequence.
ACCESSION   DAOSOU010000014 DAOSOU010000000
VERSION     DAOSOU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 18292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18292
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_18292_cov_6.330811"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..577)
                     /locus_tag="PLW15_01535"
     CDS             complement(71..577)
                     /locus_tag="PLW15_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT33113.1"
                     /translation="MATRDGTRRVPVTLDREESAPEHWLRTIRFSGFTVLMLMLVILA
                     VIVLAPNLRIFIEQRQQIAQLQQQVADAQGSVDELTKDVARWSDPAYIESQARERLFY
                     VFPGDVSYLVVGDAAASATPDGQPISDQIQATRVDWLKTLLSSVYTAGLTDAPPADLV
                     GPDVQGAQ"
     gene            complement(640..1920)
                     /gene="eno"
                     /locus_tag="PLW15_01540"
     CDS             complement(640..1920)
                     /gene="eno"
                     /locus_tag="PLW15_01540"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT33114.1"
                     /translation="MALIEAVGAREILDSRGNPTVEVEVLLEDGVVSRAAVPSGASTG
                     AFEAYELRDGDKARYGGKGVLKAVEAVLDVLGPAVEGFDAADQRLIDAEMIDLDGTDN
                     KKKLGANAILGVSLAVAKAAADSADLPLFRYLGGPNAHTLPVPMMNIINGGAHADTAL
                     DIQEFMVLPIGAESFSEALRWGTEVYHALKGELKKAGQNTGLGDEGGFAPDLPGARAA
                     LDFIVKAIETAGFTPGKDFALGLDVAATEFFANDVYSFEKQTLTPEQLTEFWVGLVKD
                     YPLATIEDPLAEDDWAGYAHLTAELGDKVQIVGDDLFVTNPTRLKRGIDEKVANSILV
                     KVNQIGTLTETLDAVAMAQRAGYTTIISHRSGETEDTTIADIAVATDSGQIKTGAPAR
                     SDRVAKYNQLLRIEEELGDAAVYAGRSAFPRFKG"
     gene            complement(1963..2721)
                     /locus_tag="PLW15_01545"
     CDS             complement(1963..2721)
                     /locus_tag="PLW15_01545"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33115.1"
                     /translation="MNYLEFDGPGSTLVFLHGGNVAGWMWGQQVPAFADHHVLVPDYP
                     GFGASNDEPWVSIAATADAVAELIATTGGPAHVVGLSLGSSVALELAARHPEQVSSLF
                     LASAQVAPPLRRDMIAARVLLAFWNQRGFWTTLARSYGLTGEDAELFVSTGLGIRRET
                     AVTIYNEISRGVPAAVLSAITVPTLVVAGEKDSSAITDASLEAVRRGIPGVMTAIAPG
                     MHHQWNIENVELFNSSLRGWLDRREVAEGLGASI"
     gene            complement(2718..3155)
                     /locus_tag="PLW15_01550"
     CDS             complement(2718..3155)
                     /locus_tag="PLW15_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOT33116.1"
                     /translation="MTDSPDITSSTAFKLHRATVLIDRIADDYLTREHGIHYAPFLVL
                     LMVRVLGEPSQQAIARNLDVSRASVTQRVSGLVESGLLEVTPDPADSRANIVRLTTPG
                     RALVDSAWRGLEHHQSGLDAGVDEPALVAQLDRIISNAEEALS"
     assembly_gap    3281..3290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3394..4671)
                     /gene="hisS"
                     /locus_tag="PLW15_01555"
     CDS             complement(3394..4671)
                     /gene="hisS"
                     /locus_tag="PLW15_01555"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT33117.1"
                     /translation="MASPVNPPRGMRDFLPAEKAQRERILSVIRASYRAHGFDEIETP
                     VVEDSERLHAGLGGDNEKLAYAVMKRGLTTEDLAAAVDPLELADLGLRFDLTVPLARF
                     YASHRSELPAVFRAIQIAPVWRAERPQKGRYRQFVQCDIDVIGESGPVAEVELITATA
                     ATVRALGLEGCVIRVNDRRLLTSMLTILGFAPEEFDGVLITVDKLDKIAQSGVLAELR
                     ERSVTPAAVDALEALFTRPVTMELLPFGEAAIRKFLPEGVDPAAVAELATIGEAVNEV
                     LGEQVVQFDPWLVRGMGYYTGAIFEVAHPSLGYSLGGGGRYDGMIGRFLGTDVPAAGF
                     SLGFERLVDLVELPETGATDAAALVHDADVPLATLAALQSALVAGGRRVRLERKPRNI
                     APLLAQLAASGFAEYAYVTAASTLDSLEFRPLA"
     gene            complement(4973..5638)
                     /locus_tag="PLW15_01560"
     CDS             complement(4973..5638)
                     /locus_tag="PLW15_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOT33118.1"
                     /translation="MTELTPPHPKLDELIAVLERLRAPGGCAWDREQTHESLVQYLVE
                     ETYELIDAIETGDTDELIEELGDVLYQVVFHSDIAAEAGQFTLEDVAAHMTAKMIGRH
                     PHVFGDTTAETAEDVMAVWDDLKKTEKPHRTSALDGIPQGMPALALADKLLGRAHKVG
                     LLDATKPGAINVTDEDELGPLLLAIVASAKANGLDSERALRTALRELQREIREHEVDP
                     PVE"
     gene            complement(5635..6234)
                     /locus_tag="PLW15_01565"
     CDS             complement(5635..6234)
                     /locus_tag="PLW15_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOT33119.1"
                     /translation="MIIEHEGVAPRIDPSAVVSPTAVISGDVRIGPDSQVLHGAVITA
                     EGGSIEIGSSVIVMENAVIRSSAANPVRIGDHCLIGPLSSISGATIGDEVFVATGVRI
                     FNGAQIDRGSEVRINAIVHLRTHLGEGSTVPIGWVAVGDPAQILPSEAHEAIWAIQKD
                     LDFPGYVFGLDRDTPDLMVQLTERYSAALGRHRTDRPIA"
     gene            complement(6269..6790)
                     /locus_tag="PLW15_01570"
     CDS             complement(6269..6790)
                     /locus_tag="PLW15_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33120.1"
                     /translation="MTAYIAPPPRIPFVLRWGLRIAQRRTGTPLLPAQLLAWYPRAAV
                     GSAVLESLIAHSDGRLTERTLKLVRMAVSFAVSCPFCVGMNSEGWEKVMSSEEREAVQ
                     GLRSLDGFDPVERLAIEYARRASATPLRFDEEFGERLREVLTEREIVVLATTAAQVNY
                     WARVIQALGCPPE"
     gene            6837..7367
                     /locus_tag="PLW15_01575"
     CDS             6837..7367
                     /locus_tag="PLW15_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOT33121.1"
                     /translation="MKFRGAVVLAIIVLAALAWFGWQALGAQAPTLTTAPGAAPATGI
                     PSDATPAVVEFVHDGDTLFLEDGRKVRLLGINTPEIGNNAECYGDEATALLRQLLPEG
                     TDVWVQADVEPLDQYGRSLLFIYTDDATNINLVLLERGAAEVEQYAPNLLLRDELYAA
                     EDAAREAGAGLWGACR"
     gene            complement(7364..8749)
                     /locus_tag="PLW15_01580"
     CDS             complement(7364..8749)
                     /locus_tag="PLW15_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33122.1"
                     /translation="MLTTFLVAIEATVLSTAVPSIVADLGDFPLFPWLFSVYLLAQAV
                     TVPVYAKLADTVGRKPIILIGIGLFLLGSILSAFAWSMPALIAFRVIQGLGAGAVLPI
                     SITIAGDIYTVEERAKVQGYLASMWAVASVVGPTLGGLFAQFDAWRGIFIVNIPLCLL
                     AAWMLTRTFHEKITRTAHRIDYAGAVLLTVALTALLLAVLEGGQAWAWNSLPSILLFG
                     GGALVLVAFLLVERRAAEPILPLWVFTRRLVLVVSIFGLGIGATLTGLTSYVPLYLQG
                     ALGVSPLIAGFALATLTVGWPIAAAVSGRLYLRIGFRNTMFIGMGLVLVGTVILAAFA
                     MTPSILVVAVSCFFVGLGMGLAATPSLILAQSSVGWNERGVVTGVNMFARNVGSAVGV
                     AVFGAIANSIFLRAGADSADPAVVTAAGSAVFIAALVVAVATLIATIAMPRTRVQQLP
                     AEETNLGLAEA"
     gene            8858..9727
                     /locus_tag="PLW15_01585"
     CDS             8858..9727
                     /locus_tag="PLW15_01585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT33123.1"
                     /translation="MLDPAWALTRPILWLALVGLLVLLVVRTAHKDRKEYQRFKRYRL
                     TARRQEMFRKWFRDSVLSIGLVSLGLLLLAWQFVAPLLQELTTWPVVRDIRGFVLHEP
                     GVTWGIIAGLVIGFVVLTAIGVRAARQDDQIPMVGDIAAMLPRNRQELRLGALLSINA
                     GIGEELMFRLALPAVIYGASGSAIAAVLGSVLLFGALHLYQGVVGVVSTTIVGAVMML
                     IYVVTGTIVVPIVLHALFDLRSLVLIPMAVHDVHRIDGVANPVTRIPGMSPTKALAKT
                     PDADVATPPAPPA"
     gene            complement(9702..>12878)
                     /gene="mfd"
                     /locus_tag="PLW15_01590"
     CDS             complement(9702..>12878)
                     /gene="mfd"
                     /locus_tag="PLW15_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT33124.1"
                     /translation="RGYNLADLSVRLVDLAYSRVDMVTRRGEFAVRGGIVDVFAPTDE
                     HPLRLEFFGDELEQLREFSVADQRSLPGDIDHAELAPSRELLLSEPVRQRAREMQHEF
                     PSLSTMLAKIGEGIPVDGMESLAPALVDRLVPLTHYLPADAAIAVLSPEKVIARAASL
                     VETNREFLAAAWNAATAGAEAPIDLDAGDFVTLASLRESAGDRPWWTMSGFDSGQGSL
                     SSVEGAYRNAPDEGLDTTASTRPADDDDEYVRIDARSVPSFAGQAEGALGHVGELLRD
                     GWTVAIVAQGHGMLDRAADLLGEHELSGRVVETLPADAEPGVAYLLQASVDAGFEVPE
                     AKLALLSESEFFGRAAGYDTRQVKRLASRRRNVVDPLQLKAGDFVVHNTHGIGKFVEL
                     VQREVSTGGRNAIKTQREFLVIEYAPNKRGYPGDKLYVPTDQLDLLTRYVGGEAPALS
                     KMGGSDWAAAKGKARKAVRDIAVELVKLYSARMASKGHAFGPDTPWQRELEEAFAFAE
                     TPDQLTTIEEVKADMERPIPMDRLLSGDVGYGKTEVAVRAAFKAVQDGKQVAMIVPTT
                     LLVRQHMETFAERFAGFPVHLRALSRFQTDKESKETIAGLADGTVDVVIGTHRLLSQG
                     IGFKDLGLVIIDEEQRFGVEHKDALKKLKTNVDILAMTATPIPRTLEMAVTGIREMST
                     LATPPEDRHPILSFVGPYSDRQVAAAIRRELLREGQVFYVHNRVSSINRVASQLAELV
                     PEARIAVAHGQLPEAVLEQVMVDFWERKFDVLVSTTIIETGLDIANANTLIIDRADKY
                     GLSQLHQLRGRVGRGRERAYAYFLYDELKPLSETAHERLNTIAANNELGAGMQIALKD
                     LEIRGAGNLLGGEQSGHIAGVGFDLYLRMIGEAVSTFRGDVAEGQTELRLELPVDAHL
                     PEEYIESERLRLEAYQKLSTASAPTATDDSIDRVLEELTDRYGEPPEQVLNLVAVSRL
                     RRMAQKSGLSEVVVMGDKLRIAPADLADSIQVRLQRMYPGARYHAAAAAVLVPLPPEP
                     TDTQLIEWASGLLVAIFGAETTPAALAE"
     assembly_gap    12879..12978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13431..13799)
                     /locus_tag="PLW15_01595"
     CDS             complement(13431..13799)
                     /locus_tag="PLW15_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HOT33125.1"
                     /translation="MTSPEIDPVIDPVVSNPGLYSVIFENDRVRVLEYLDEPGAESVP
                     HDHPDSVMITLTAFERRLSAGDRTVDVEMPAATARWLAAQRHSGRNTGTTPTHCIFVE
                     LKEPAPVPPTGPAVLGPTAS"
     gene            complement(13856..14443)
                     /gene="pth"
                     /locus_tag="PLW15_01600"
     CDS             complement(13856..14443)
                     /gene="pth"
                     /locus_tag="PLW15_01600"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT33126.1"
                     /translation="MDDDIWLVVGLGNPGPGYAGNRHNVGQMALAQLADRASLTFKNH
                     KANAAVAEGRSFAAAPKLVLAKPNTYMNVSGGPVAALLRFFKVPPARLIVLHDELDIP
                     FDSVRLKFGGGHGGHNGLRDIIAATGTGDFTRVRIGIGRPPGRQPAADFVLKDFTGAE
                     REVLPSLLSDAADATELIASDGLTAAQLKFHSPRE"
     gene            complement(14464..15117)
                     /locus_tag="PLW15_01605"
     CDS             complement(14464..15117)
                     /locus_tag="PLW15_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOT33127.1"
                     /translation="MADKAQSENSNHLLTEIRDSFGKGAARKIRAKGKIPAVIYGHGT
                     EPQHVTLPGHEVGLILRKSNQVLELDIQGKIQLALVKDVQKDPVRQIIEHIDLIVVRK
                     GEKVTVDVPVHLEGESYSGTIAMLDAQTISLEVEATHIPERVVVDIEGLTEGVHITAK
                     DLVLPTGATLLSDPDLLIVGITHPQAASAADQEADAALAAEGAAQSAAAAAAAEDAE"
     gene            complement(15249..15749)
                     /locus_tag="PLW15_01610"
     CDS             complement(15249..15749)
                     /locus_tag="PLW15_01610"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HOT33128.1"
                     /translation="MPRPPLVVVMGVSGSGKSTIGSLLADHLGVPFIDADDLHPASNV
                     AKMSAGEPLTDGDRWPWLALVGEALAAASATGLVLACSALKRSYREAILAGAPGALFV
                     QLDGSRELLAHRMGARTDHFMPTTLLDSQLATLESLQPEEPGFTVGIEQEPGEIVEEI
                     ADRLTH"
     gene            15836..17284
                     /gene="gndA"
                     /locus_tag="PLW15_01615"
     CDS             15836..17284
                     /gene="gndA"
                     /locus_tag="PLW15_01615"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOT33129.1"
                     /translation="MAQANIGVVGLAVMGSNLARNLASREGNTVAVYNRTWARTETLL
                     EEHPEAGFIASKTIEEFAASLAKPRTALIMVQAGKGTDAVIDELVAVFEPGDIIVDGG
                     NALFTDTIRREKAVRETGINFVGAGISGGEEGALNGPSIMPGGSDESWVTLGPILKSI
                     AAIAEGEPCVTHVGHDGAGHFVKMIHNGIEYADMQLIAEAYDLIRRGTGKTPAEISEI
                     FTEWNKGELESYLIEITAEVLKQVDAKTGKPLVDVITDQAGSKGTGVWTVQTALDLGI
                     PVSGIAEAVFARALSSKPAQRAAAASLPGPSEDSWKPGDPDAFIEGVRLALYASKVIA
                     YSQGFDAIVAGAEQYGWDIKKGEIAKIWRGGCIIRARFLNRITEAYAENPGLVALVTA
                     PFFVDVMAKAQTAWRNVVADAAHAGIPTPAFSSSLAYYDGLRADRLPAALVQGQRDFF
                     GAHTYQRVDMPGTFHTLWSGDRTEVESAGSSH"
     gene            complement(17281..>18292)
                     /locus_tag="PLW15_01620"
     CDS             complement(17281..>18292)
                     /locus_tag="PLW15_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HOT33130.1"
                     /translation="AFDTDDWFYRITTFVQMIGVVVLALGIPAAFRSLLEGDHLDNGV
                     IVAGYVVMRIAMVAQWLRVARQDPAHRRTALTYVTYILIAQVGWVVLALLPVSVGTFV
                     MFAFVLYAIELGGPVVAERKVGGTPWHPHHIAERYGLLAIIALGEAVFGTVASVAALV
                     EDQGWSLEAALVVFAGVGITFGLWWTYFIMPSGVVLARHRSRGFVWGYGHILIYAAIA
                     ATGAGLHVVAFVIEGHTDIGTVGAIVAIAVPVLVFSVVNFALYAYLVRQWDSLHAVLF
                     AGTVLMLVLAVALAAAGASLGVCLVIVTLSPAVVVVGFETVGHRHQAASLERALEEPT
                     AP"
BASE COUNT         3014 a         6042 c         6270 g         2856 t
ORIGIN      
        1 cgacgagctc gatcaccggg gaggcgggct cgatcaccgg gggggatctc gacgagctcg
       61 atcaccgggg tcactgggcg ccctgaacgt cggggcccac gagatcggcg ggcggggcat
      121 ccgtgaggcc ggcggtgtag accgacgaga gcagcgtctt cagccagtcg acccgggtgg
      181 cctggatctg gtcgctgatg ggctggccat cgggggtggc ggatgccgcg gcatccccca
      241 cgacgaggta gctcacgtcg cccgggaaga cgtagaacaa ccgctcccgc gcctgggact
      301 cgatgtaggc gggatcactc cacctcgcca cgtccttggt gagctcgtcg acgctgccct
      361 gagcgtcggc gacctgctgc tgcaactggg cgatctgctg gcgctgctcg atgaagatcc
      421 gcagattggg ggccagcacg atcaccgcga ggatcaccag catgagcatc agcacggtga
      481 accccgagaa gcggatcgtg cgcaaccagt gctcgggcgc gctctcctcg cgatcgaggg
      541 tgacggggac ccggcgggtt ccgtctcgcg tagccatggg ttcgatgtta accgctgatc
      601 gagcttgccg agatctcgac aagctcgatc agcgaatgat cagcccttga agcgcgggaa
      661 cgccgagcga ccggcgtaga cggcggcgtc gcccagctcc tcctcgatgc ggagcagctg
      721 gttgtacttg gcaacccggt cgctgcgggc gggggcgccc gtcttgatct ggccggagtc
      781 ggtggccacc gcgatgtcgg cgatcgtggt gtcctcggtc tcgccggagc ggtgcgagat
      841 gatcgtcgtg tagccggcac gctgggccat ggccacggcg tcgagcgtct cggtgagcgt
      901 gccgatctgg ttgaccttca cgaggatcga gttggcgacc ttctcgtcga tgccgcgctt
      961 gaggcgggtc gggttcgtca cgaacaggtc gtcaccgacg atctgcacct tgtcgccgag
     1021 ctcggcggtg aggtgggcgt agccggccca gtcgtcctcg gcgagcgggt cctcgatggt
     1081 cgccagcggg tagtccttga ccagacccac ccagaactcg gtgagctgct cgggggtcag
     1141 cgtctgcttc tcgaaggagt acacgtcgtt ggcgaagaac tcggtggccg cgacatccag
     1201 tcccagggcg aagtccttgc ccggggtgaa gccggcggtc tcgatggcct tgacgatgaa
     1261 gtcgagcgcg gcgcgagcac cgggaaggtc gggggcgaag ccaccctcgt cgccgaggcc
     1321 ggtgttctgg cccgccttct tcagctcgcc cttgagagcg tggtacacct cggtgcccca
     1381 gcgaagagcc tcggagaacg actcggcgcc gatcggcagc accatgaact cctggatgtc
     1441 gagggccgtg tcggcgtgcg cgccaccgtt gatgatgttc atcatcggca ccggcagggt
     1501 gtgggcgttc gggccaccga ggtagcggaa cagcggcagg tcggccgagt cggcggcagc
     1561 cttggcaaca gcgagcgaga cgccgaggat cgcgttggcg cccagcttct tcttgttgtc
     1621 ggtgccgtcg aggtcgatca tctcggcgtc gatgaggcgc tggtctgccg cgtcgaagcc
     1681 ctcgaccgcg gggccgagaa cgtcgagcac cgcctcgacg gccttgagca cgcccttgcc
     1741 gccgtagcgg gccttgtcgc cgtcgcgcag ctcgtacgcc tcgaaggcac cggtggatgc
     1801 accggagggc acggcggcgc gggagaccac gccgtcctcg agcagaacct cgacctcaac
     1861 ggtcgggttg ccccgggaat caagaatctc gcgggcgcca actgcctcga tcaaggccat
     1921 gggggaactc ctcaaatagg aaaaacgtgt ggtgggtttc gcctaaatac tagcgccgag
     1981 cccctcggcc acctcgcgcc ggtcgagcca cccacgcaac gagctgttga acagttcgac
     2041 gttctcgatg ttccactggt gatgcatccc cggtgcgatc gctgtcatga caccggggat
     2101 gccacgacgc accgcctcaa gggaggcgtc agtgatggcg ctcgaatcct tctcgccggc
     2161 aaccacgagg gtgggaacag tgatggcaga caagactgcg gcgggcacgc ctcgactgat
     2221 ctcgttgtag atggtcacgg cggtttcgcg acggataccc agaccggtcg acacgaacag
     2281 ttccgcatcc tcccccgtca gcccgtagct gcgcgcaagt gtcgtccaga aaccgcgctg
     2341 attccagaac gcgagcagca cccgcgcggc gatcatgtcg cgacgtaacg gtggcgcgac
     2401 ctgggcgctg gcgagaaaca ggctcgagac ctgctccgga tgcctcgccg caagttccag
     2461 cgcgacgctc gacccgagcg acagccccac gacgtgagcc ggcccgccgg tcgtcgcgat
     2521 cagctcagcc accgcgtctg cggtagcggc gatcgagacc cagggctcgt cgttgctcgc
     2581 cccgaagccc ggatagtcgg gcaccagcac gtggtggtcg gcgaacgccg gaacctgctg
     2641 tccccacatc cacccggcga cgttgccgcc gtgcaggaag acgagggtgg aacccggtcc
     2701 gtcgaattcg agatagttca tgagagtgcc tcctcggcgt tggaaatgat tcggtcaagt
     2761 tgggcgacca gcgcgggctc gtcgacgccc gcgtcgaggc cgctctggtg atgttcgagc
     2821 ccacgccacg cactgtcgac gagcgccctg cctggcgtgg tgagacgcac gatgttcgcc
     2881 cgcgagtcgg cgggatcggg ggtgacttcg agcagcccac tctcgaccag ccccgacacc
     2941 cgctgggtca cgctcgcgcg agacacgtcg agattgcggg cgatggcctg ctggctgggc
     3001 tctcccagca cgcgaaccat gagcagcaca aggaacggcg cgtagtggat gccgtgctcg
     3061 cgggtgaggt agtcgtcggc gatgcggtcg atgagcacgg tcgcgcgatg gagcttgaac
     3121 gcggtgctcg aggtgatgtc gggtgagtcg gtcatattgt taatagctta acaatagtgc
     3181 agcggggagt caatgatctc gacaggctcg atcaccgggg cgctctcgat cagtggatct
     3241 cgacgagcgg gatctcgaca ggctcgatca ccggggcgct nnnnnnnnnn tgaacgcggt
     3301 gctggaggtg atatcgggtg tggtcgtcat attgttaata gattaacaat aatgttcgag
     3361 agggtcaatg atctcgacga gctcgatcac cgatcaggcg agcgggcgga actccagcga
     3421 gtcgagcgtc gacgccgccg tcacgtaggc gtactcggcg aacccggatg ccgcgagctg
     3481 agccagcagg ggtgcgatgt tgcgcggctt gcgttcgagt cgcacccgcc gtccacccgc
     3541 gacgagggcc gactgcaggg cggcgagggt agccaggggc acgtcagcgt cgtggaccag
     3601 cgccgcggca tccgtcgccc ccgtctcggg aagctcgacc aggtcgacga gacgctcgaa
     3661 cccgagtgag aagccggcag cgggaacgtc ggtgccgagg aagcgaccga tcatgccgtc
     3721 gtagcgtccg ccgccgccga gcgagtagcc gagcgacggg tgcgcgacct cgaagatcgc
     3781 gcccgtgtag tagcccatgc cccgcaccag ccacgggtcg aactgcacga cctgctcgcc
     3841 gagcacctcg ttgacggcct cgccgatggt tgccagttcc gcgacagcgg ccgggtccac
     3901 gccctccggc aggaacttcc tgatcgcggc ctcaccgaac ggcagcagct ccatcgtgac
     3961 cggccgcgtg aagagcgcct ccagcgcgtc cacggcggcg ggagtgacgg agcgctcccg
     4021 cagctccgcg agcacaccgg actgggcgat cttgtccagc ttgtccacgg tgatcagcac
     4081 accgtcgaac tcctcgggag cgaacccgag gatcgtgagc atgctcgtga gcagccgacg
     4141 atcgttgacc cggatgacgc atccctcaag cccgagcgcc ctcaccgttg ccgcggtcgc
     4201 cgtgatcaac tcaacctcgg cgaccggccc ggactccccg atcacatcga tgtcgcactg
     4261 cacgaactgg cggtagcgac ccttctgggg ccgctcggcc cgccacaccg gcgcgatctg
     4321 gatggcacgg aagaccgcgg gcagttctga ccggtggctc gcgtagaacc gagcgagggg
     4381 cacggtgagg tcgaagcgca gcccaagatc ggcgagctcg agcggatcga cggcggccgc
     4441 gaggtcttcg gtcgtcagac cgcgcttcat caccgcatag gcgagcttct cgttgtctcc
     4501 gccgaggccg gcgtgcaggc gctcactgtc ttcgacaacg ggcgtttcga tctcgtcgaa
     4561 accgtgcgcc cgatagctgg cacggatgac actcaggatg cgctcacgct gagccttctc
     4621 ggccggcagg aagtcgcgca tcccgcgcgg aggattcacg ggtgacgcca tgcgtcgatt
     4681 gtgtcaggtc gtgcgtcgtg gcgttggcgt cgaccggcgc cggcacgtgt aaaattgagg
     4741 cttcgcgcct gtcgatgatg gtccgggcgc gagtcgaagc atccacttcg ccacagtcga
     4801 aaccgacggc tcgggtaagc ccgccggttt ctcgggcgca gcatccctcg tggacgcgcg
     4861 tccgggtgcc ctctccattc gggttgggga gggcaccgaa tcccgcgaca gccggttgag
     4921 taggtgccca caccgtatcg aaaccgctta actggtctcg atacgcctgc ggctactcga
     4981 ccggcgggtc aacttcgtgc tcgcggatct cccgctggag ttcgcgcagc gccgtgcgca
     5041 gggcgcgctc cgagtccagc ccattcgcct ttgcggatgc cacgatcgcg agaagcagcg
     5101 gaccgagttc gtcttcgtcg gtgacgttga tcgcgcccgg cttcgtggca tccagaaggc
     5161 ccaccttgtg cgcgcgcccg aggagcttgt ccgcgagcgc gagcgcgggc atcccctggg
     5221 gtatcccgtc gagggcactc gtgcggtgcg gcttctcggt cttcttgagg tcgtcccaga
     5281 cggccatgac gtcttccgcg gtctcggccg tggtgtcgcc gaagacgtgc gggtggcgac
     5341 cgatcatctt cgcggtcatg tgcgcggcga catcctcgag ggtgaactgg ccggcctcgg
     5401 cagcgatgtc gctgtggaag accacctggt agagcacgtc gccgagctcc tcgatgagtt
     5461 cgtcggtatc gcccgtctcg atcgcgtcaa tcagctcgta cgtctcctcg acgaggtact
     5521 gcacgagcga ctcgtgggtc tgctcgcgat cccaggcaca gccgcccggt gcgcgcagcc
     5581 gctcgagtac ggcgatcagc tcatcgagct tcggatgcgg cggcgtgagc tcggtcacgc
     5641 gatcggccga tcggtgcggt gccggcctag cgcggcggag taccgctcgg tcagttgcac
     5701 catgaggtcg ggggtgtcgc ggtcgagtcc gaatacgtag ccggggaagt cgaggtcctt
     5761 ctggatcgcc cagatcgcct cgtgcgcctc ggagggcagg atctgggcgg ggtcaccgac
     5821 ggccacccag ccgatcggta ccgtcgaccc ctcacccagg tgggtgcgca ggtgcacgat
     5881 cgcgttgatg cgcacctcgc tgccacggtc gatctgagct ccgttgaaga tgcgcacgcc
     5941 ggtcgcgacg aacacctcat cgccgattgt cgcgcccgag atcgatgaga gcggcccgat
     6001 caggcagtgg tcgccgatcc gcaccggatt cgcggcgctg gagcggatga cggcgttctc
     6061 catcacgatc acgctcgagc cgatctcgat cgatccaccc tccgcggtga tcaccgcacc
     6121 gtggagtact tggctgtcag ggccgatccg tacgtctccg ctgatgaccg cagtgggcga
     6181 gacgaccgcg ctggggtcga tgcgcggggc aactccctcg tgttcgatga tcacggggcc
     6241 agactaccca gagcggcgac tcggtggatc attcgggcgg gcatcccaga gcctggatga
     6301 cgcgcgccca gtagttgacc tgcgccgcag tggtcgcgag cacgacgatc tcgcgttcgg
     6361 tgagaacctc gcgaagccgc tcgccgaact cctcgtcgaa ccgcagcggc gtcgcgctgg
     6421 cgcggcgggc gtactcgatg gcgaggcgtt cgaccggatc gaagccgtcg agcgaacgaa
     6481 ggccctgcac ggcctcgcgc tcctcagacg acatgacctt ctcccagccc tccgagttca
     6541 tgccgacgca gaaggggcaa ctcaccgcga acgacaccgc catgcgcacg agcttcaggg
     6601 tgcgctcggt gaggcgaccg tcgctgtgag cgatgagtga ctcgagcacg gccgaaccga
     6661 ccgcggccct cggataccac gcgagcagtt gggcgggcag cagcggtgtc cccgtgcgtc
     6721 gctgggcgat gcgcagaccc caccgcagga cgaaggggat gcgcggaggt ggggcgatgt
     6781 acgcggtcat acccccagtc tccaccggcg acaccggtcg ctggcagact ggtgccgtga
     6841 agttcagggg cgccgtcgtg ctggccatca tcgtgctggc cgcgctggcg tggttcgggt
     6901 ggcaggccct cggggcgcag gcgccgacgc tgaccacggc gcccggcgcg gcgcctgcca
     6961 cgggcatccc gagcgatgcg acaccggcgg tcgtcgaatt cgtgcacgac ggcgacacgc
     7021 tcttcctcga agacggacgc aaggtgcggc tgctcggaat caacaccccg gagatcggca
     7081 acaacgccga gtgctacggc gatgaggcga ccgctctgct gcggcagcta ctgcccgagg
     7141 gcaccgacgt gtgggtgcag gccgacgtcg agcccctcga ccagtacggt cggtctctgc
     7201 tgttcatcta cacggatgac gcgacgaaca tcaacctcgt actgctcgag cgcggcgccg
     7261 ccgaggtcga gcagtacgct cccaacctgc tgctgcgcga cgagctgtac gccgcggagg
     7321 atgccgcgcg cgaggccgga gccggcctct ggggagcgtg ccgctaggcc tcggccaggc
     7381 ccaggttcgt ctcctctgcc ggtaactgct gcacccgggt gcgcggcatc gcgatggtcg
     7441 cgatgagggt cgccaccgcg acgacgaggg ccgcgatgaa cacggccgaa cccgccgcgg
     7501 tcaccacagc cgggtcggct gaatcggccc ccgcgcggag gaagatcgag ttagcgatcg
     7561 ccccgaacac tgcaacgccc accgcgcttc ccacgttgcg cgcgaacatg ttcactcccg
     7621 tgacgacgcc gcgctcgttc cagccgacgc tggattgggc gaggatcagg ctcggagtag
     7681 ccgcgagacc catgccgagt cccacgaaga agcagctcac ggcgaccacg aggatcgagg
     7741 gcgtcatcgc gaatgccgcg aggatcaccg tgcccaccag caccaaaccc attccgatga
     7801 acatggtgtt gcggaagccg atgcgcaggt agaggcgacc cgagaccgcg gccgcgatcg
     7861 gccagccgac ggtgagggtt gcgagcgcga atccagcgat gagcggtgac acccccaacg
     7921 ccccctgcag gtagagcgga acgtagctgg tcagacccgt gagcgtcgcg ccgatcccga
     7981 ggccgaagat cgagacgacc agcaccagcc ggcgcgtgaa gacccacagc ggcaggatcg
     8041 gttcggccgc ccgacgctcg accagcagga acgccaccag cacgagggct cctccaccga
     8101 acagcaggat gctgggcagc gagttccacg cccacgcctg accgccctcg agcaccgcga
     8161 gcagcagcgc ggtcagagcc acagtgagca gcaccgcacc ggcgtagtcg atgcggtggg
     8221 ccgtgcgggt gatcttctcg tggaaggtgc gggtgagcat ccatgccgcc agtaggcaga
     8281 gcggaatgtt gacgatgaag attccccgcc aggcatcgaa ctgggcgaac aggccaccga
     8341 gggtcgggcc gacgacggat gcgacggccc acatgctcgc gaggtagccc tgcaccttgg
     8401 cgcgctcctc gaccgtgtag atgtcaccgg cgatggtgat ggatatcggc agcacggccc
     8461 cggcgcccag cccctgaatc acgcggaacg cgatgagcgc cggcatgctc cacgcgaagg
     8521 cgctgagaat cgagccgagc aggaagagcc cgatgccgat gaggatgatc ggcttgcgcc
     8581 cgacggtgtc ggcaagcttt gcgtagaccg gaacggtgac cgcttgcgcc aggaggtaca
     8641 cggagaacag ccacgggaac agcgggaagt cacccagatc ggcgacgatc gacggcacgg
     8701 cggtcgagag caccgtcgcc tcgatcgcga ccaggaacgt cgtgagcatg agagcgatga
     8761 ggatggggcc gcgctccgag cgcaggccga cgttgatggt cacggagcaa tgcaaccacg
     8821 cggcggcatc agtgcattcc cgtgggagca ttgattcatg ctcgaccccg cctgggcgct
     8881 cacgcgaccg atcctctggc tcgcactcgt cgggctgctc gtactgctgg tcgttcgcac
     8941 cgcgcacaag gaccgcaagg agtaccagag gttcaagcgg taccgactga cggccaggcg
     9001 ccaggagatg ttccgcaaat ggttccgcga ctccgtgctg agcatcgggc tggtgagcct
     9061 cgggctgctg ctgctcgcgt ggcagttcgt cgcgccgctg ctgcaggagc tgacgacgtg
     9121 gccggtggtg cgcgacatcc ggggattcgt gctccacgag ccgggcgtga cgtggggaat
     9181 catcgcgggc ctggtcatcg gattcgtcgt gctcacggcg atcggcgtgc gtgcagcccg
     9241 tcaggacgac cagatcccga tggtcggtga catcgcggcc atgctcccgc gcaaccggca
     9301 ggagctgcgg ctgggtgccc tgttgtcgat caacgcgggg atcggcgagg agctgatgtt
     9361 caggctggcc ctgcccgcgg tgatctacgg cgcgagcggc agcgcgatcg cggccgtgct
     9421 gggaagtgtg cttctgttcg gcgctctgca cctgtaccag ggtgtggtcg gcgtggtgag
     9481 caccacgatc gtgggtgccg tcatgatgct gatctacgtc gtcaccggaa cgatcgtggt
     9541 gccgatagtg ctgcacgccc tgttcgacct gcggtcgctc gtgctcatcc ccatggccgt
     9601 gcacgatgtg catcgcatcg acggtgtggc caacccggtg acgagaatcc ccggcatgag
     9661 tccgacgaag gccctggcga aaaccccgga cgcggatgtc gctactccgc cagcgccgcc
     9721 ggcgtagtct cggcaccgaa gatcgccacg agcaacccgc tcgcccactc gatcagctgc
     9781 gtatccgtgg gctcgggcgg cagcggcacg agcaccgcgg ccgccgccgc gtggtaccgg
     9841 gcgcccgggt acatccgctg cagtcgcacc tggatcgagt cggcgaggtc ggccggcgct
     9901 atgcgcagct tgtcgcccat gacgacgacc tcgctgagtc cggacttctg cgccatccgt
     9961 cgcagccgcg agaccgccac gaggttcagc acctgctcgg gcggctcgcc gtagcggtcg
    10021 gtgagctctt cgagcacgcg gtcgatcgag tcatccgtcg ccgtcggggc gctcgcggtc
    10081 gagagcttct ggtaggcctc gagacgcagg cgctccgatt cgatgtactc ctcgggcagg
    10141 tgcgcgtcga cgggcagctc gagccgcagc tcggtctggc cctcggcgac gtcgccacgg
    10201 aaggtcgaca cggcctcgcc gatcatgcgc aggtagaggt cgaagccgac acccgcgatg
    10261 tgtccggact gctcgccgcc gagcaggttg cccgcaccgc ggatctcgag gtccttgagg
    10321 gcgatctgca taccggcccc gagttcgttg ttcgcggcga tcgtgttgag gcgctcgtgc
    10381 gcggtctcgc tgagcggctt gagctcgtcg tagaggaagt aggcgtacgc ccgctcccgg
    10441 ccacgaccga cccggccgcg cagctggtgc agctggctga ggccgtactt gtcggcccgg
    10501 tcgatgatga gggtgttcgc gttggcgatg tcgagaccgg tctcgatgat ggtggtcgag
    10561 acgagcacgt cgaacttgcg ttcccagaaa tcgaccatca cctgctcgag caccgcctcc
    10621 ggcagctgac cgtgcgcgac ggcgatgcgt gcctccggca cgagctccgc cagctgggat
    10681 gccacgcggt tgatgctcga gacccggttg tgcacgtaga acacctggcc ctcgcgcagc
    10741 agctcacgac ggatcgccgc ggcgacctgc cggtcggagt agggacccac gaacgacagg
    10801 atcgggtggc ggtcctccgg tggtgtcgcc agagtcgaca tctcccggat gcccgtgacc
    10861 gccatctcga gcgtgcgcgg gatcggcgtc gccgtcatcg cgaggatgtc gacgttggtc
    10921 ttgagcttct tcagcgcgtc tttgtgctcg accccgaaac gctgctcctc gtcgatgatc
    10981 acgagcccga gatccttgaa cccgatgccc tggctgagca accggtgcgt gccgatcacg
    11041 acatcgacgg tgccgtcggc gaggcccgcg atcgtctcct tcgactcctt gtcggtctgg
    11101 aatcggctga gcgcccgcag gtgcacgggg aagccggcga accgctcggc gaaggtctcc
    11161 atgtgctggc gcacgagcag ggtcgtgggc acgatcatgg cgacctgctt gccgtcctgc
    11221 accgccttga acgccgctcg cacggcaacc tcggtcttgc cgtagccgac gtcgcccgac
    11281 agcagccggt ccatcgggat cggccgctcc atgtcagcct tgacctcctc gatggtggtc
    11341 agctggtcgg gcgtctcggc gaacgcgaag gcctcctcca gctcgcgctg ccacggggtg
    11401 tcgggcccga aggcgtggcc cttgctcgcc atgcgggctg agtagagctt cacgagctcg
    11461 accgcgatgt ctcgcaccgc cttgcgagcc ttgcccttgg ctgcggccca gtcgctgccg
    11521 cccatcttgc tgagggccgg cgcctcacca ccgacgtagc gggtgagcag gtcgagctgg
    11581 tcggtcggca cgtagagctt gtcgcccggg tagccgcgct tgttgggcgc gtactcgatc
    11641 acgaggaact cgcgctgcgt cttgatcgcg ttgcggccgc cggttgacac ctcgcgctgc
    11701 accagctcga cgaacttgcc gatgccgtgc gtgttgtgca ccacgaagtc gccagccttg
    11761 agctgcagcg ggtcgacgac gttgcggcgg cgactggcca accgcttgac ctgcctcgtg
    11821 tcgtagccgg cggcgcgacc gaagaactcg ctctcgctca gcagagccag cttcgcctcc
    11881 ggaacctcga agcccgcatc gacggatgcc tgcagcaggt acgcgacgcc gggctcggca
    11941 tccgcgggca gggtctccac cacgcggccg gatagctcgt gctcgcccag gaggtcggcc
    12001 gcacgatcga gcatgccgtg accctgggcc acgatggcga cagtccagcc gtcgcggagc
    12061 agctcgccga catggccgag ggcaccctcg gcctggcccg cgaagctcgg caccgagcgg
    12121 gcatcgatgc gcacgtactc gtcgtcgtcg tccgccggtc gagtagacgc agtcgtatcg
    12181 agaccctcgt cgggcgcgtt tcgatacgcg ccttcgacgc tactcaacga gccttgacca
    12241 ctgtcgaagc cactcatcgt ccaccacggg cgatcgcccg cggattcgcg caggctcgcg
    12301 agcgtgacga agtctccagc gtcgaggtcg atcggcgcct cagcaccggc ggtggccgcg
    12361 ttccacgcgg ccgccaggaa ctcgcggttg gtctccacca ggctggccgc gcgcgcgatg
    12421 accttctccg gtgagagcac ggcgatcgcg gcatccgctg gcaggtagtg ggtcagggga
    12481 acgagccggt ccaccaatgc gggcgcgagc gactccatgc cgtcgaccgg gatgccctca
    12541 ccgatcttcg cgagcatcgt ggacaggctc gggaactcgt gctgcatctc ccgcgcccgc
    12601 tggcgcacgg gctcgctcag cagcagttcg cggctgggtg cgagctcggc gtggtcgatg
    12661 tcgccgggca gggaacgctg gtcggcaacg gagaactcgc gcagctgctc cagctcgtcg
    12721 ccgaagaact ccaggcgcag cggatgctcg tcggtgggcg cgaacacgtc cacgatgcca
    12781 ccgcgcacgg cgaactctcc gcggcgcgtc accatgtcga cgcgcgagta ggcgagatcg
    12841 accaaccgca cgctcaggtc ggcgaggttg tagccgcgnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnncg ggcgggcatc cgtcgtcagg aggccggcga tgagcggcgc
    13021 gcggaggccg tcgacgagcg agaaatcggc gcttcgggcg ctctgcgcaa gggcactctg
    13081 gaacgtgcgg gcgcgcgaaa gccccgtgat caatccgttc agagtcaccg cacgagtcta
    13141 cgtggcgcgg gtgacgtcgc cgccgaagct cagtcgagcg tcactctcga cgctgcagct
    13201 agggccaccg cgtagcgccg tgatcccgcg gcaatccgcc ccgcgcgact ccgcgaactc
    13261 aggacattgg taaggggagc aaacgaatgt cctgagttcg gggagtcagc gaagggtgag
    13321 gaacaacttg gcagtcggcc gcatcgcggg tctacacagt cggggcctag gcagtgcggg
    13381 tgaccagggt tcgactgcgc ccggcgcgga gggccgggcg cgaaccgacc tcaggacgcg
    13441 gtcgggccga gcaccgccgg gccggtcggc ggcacgggcg caggttcctt caactccacg
    13501 aagatgcagt gggtcggggt ggtgccggtg ttgcggccac tgtgcctctg cgcggcgagc
    13561 caccgggcgg tcgccgcagg catctcaacg tcgacggtgc gatctccggc cgagagtcga
    13621 cgttcgaacg ccgtgagcgt gatcatcaca ctgtcgggat gatcgtgggg aacggactcg
    13681 gcgcccggct catccaggta ctcgagtact cgcacacgat cgttctcgaa gatcacggag
    13741 tacagcccgg ggttgctgac cacggggtcg atcacggggt cgatctcggg gctcgtcatt
    13801 ccccgacgct acgcccggga tgccgaggcg gcaagggcgg gcgggcgccc gcgcgctact
    13861 cgcgcggcga gtggaacttg agctgggctg ccgtgagtcc gtcgctggcg atgagctcgg
    13921 tggcatccgc tgcgtcgctc aacaacgacg gcagtacctc gcgctcggcg ccggtgaagt
    13981 ccttaagcac gaagtcggcc gcgggctgac gaccgggcgg gcggccgatg ccgatgcgca
    14041 cgcgcgtgaa atccccggtg ccggtggccg cgatgatgtc gcgaaggccg ttgtggccgc
    14101 cgtgcccgcc gccgaacttc aggcgcacgc tgtcgaacgg aatgtccagc tcatcgtgca
    14161 gcacgatcag cctcgccgga ggcaccttga agaaccgcag cagcgccgcg accgggccgc
    14221 ccgacacgtt catgtaggtg ttgggcttgg ccagaaccag cttcggcgcg gcggcaaaac
    14281 tgcgcccctc ggcgacggcc gcgttggcct tgtggttctt gaaggtcagg gatgcccggt
    14341 cggccagctg cgcgagagcc atctgcccga cgttgtgacg atttccggcg tagccgggcc
    14401 cggggttacc gagcccgact accaaccaga tgtcgtcgtc cacgacccgt ctgccgaaga
    14461 tgactactcg gcgtcctcgg cagcggcagc cgcggcggcc gactgggcgg caccctcagc
    14521 agcgagcgcg gcgtcggcct cctggtcggc ggcgctggcg gcctgcgggt gggtgatgcc
    14581 gacgatgagc agatcggggt cggagagcag ggtcgcgccg gtcgggagca cgaggtcctt
    14641 ggccgtgatg tgcacaccct cggtcaggcc ctcgatgtcg acgacaacgc gctcggggat
    14701 gtgagtggcc tccacctcga gcgagatcgt ctgggcgtcg agcatggcga tcgtgccgga
    14761 gtaggactcg ccctcgaggt gcacgggcac gtcgacggtg accttctcac ccttgcgcac
    14821 gacgatgagg tcgatgtgct cgatgatctg gcgcaccggg tccttctgca cgtccttgac
    14881 gagcgcgagc tggatcttgc cctggatgtc gagctccagc acctggttcg acttgcgcag
    14941 gatcagtccg acctcgtggc cgggcagggt gacgtgctgg ggctcggtgc cgtgaccgta
    15001 gatgacggcc gggatcttgc ccttggcgcg gatcttgcga gccgcaccct tgccgaagct
    15061 gtcgcggatc tccgtgagca ggtggttgct gttctcagac tgtgccttgt cggccatgat
    15121 gattctccat tgtgggcggc tgaggccctg tcggtatgtt gttcaactcg aacgccgcag
    15181 cgtgaggaaa agcccgagga ctcgcccacc gcgtcgataa cggatgccga tgcatccctc
    15241 gccgaagttc agtgggtgag tctatcagcg atttcctcga cgatctcgcc gggttcctgc
    15301 tcgatgccga cggtgaagcc cggttcctcg ggctgcagtg actccagcgt cgcgagctgg
    15361 gaatcgagaa gggtcgtggg catgaagtgg tcggtgcgcg ctcccatgcg gtgcgcgagc
    15421 aactcgcgcg agccatcgag ctgcacgaac agcgcccccg gcgccccggc caggatcgcc
    15481 tcccggtagc tgcgcttgag cgccgagcag gcgaggacga gcccggtggc ggatgccgcg
    15541 gcgagcgcct ccccgacgag tgcgagccac ggccaccggt cgccgtcggt gaggggctca
    15601 cccgccgaca tcttcgcgac gttggatgcg gggtgcaggt cgtccgcgtc gatgaacggc
    15661 accccgaggt ggtcggccag cagtgacccg atggtggact tgcccgagcc gctcacaccc
    15721 atgacgacga cgagcggggg ccggggcatc cccccacgat agcgatagcc ttgagcccgg
    15781 tttcgatacg cggctgcgcc gctactcaac cagcgaacta caagggagag aaacagtggc
    15841 acaggcgaac atcggcgttg tcggacttgc ggtgatggga tccaacctcg cccgcaacct
    15901 cgcgagccgc gagggcaaca cggtcgcggt gtacaaccgc acctgggcgc gcaccgagac
    15961 cctgctcgag gagcaccccg aggcgggctt catcgcgtcg aagaccatcg aggagttcgc
    16021 cgcgtcgctc gccaagcccc gcaccgcgct catcatggtg caggcgggca agggcaccga
    16081 cgccgtcatc gacgagctcg tcgcggtgtt cgagcccggc gacatcatcg tcgacggcgg
    16141 caacgcgctg ttcaccgaca cgatccgccg cgagaaggcg gtgcgcgaaa ccggcatcaa
    16201 cttcgtcggc gccggcatct ccggtgggga ggagggcgcc ctcaacggcc cctcgatcat
    16261 gcccggcggc tcggatgagt cgtgggtgac cctcggcccc atcctcaagt cgatcgccgc
    16321 catcgccgag ggcgagccct gtgtcacgca cgtcggacac gacggcgccg gccacttcgt
    16381 caagatgatc cacaacggca tcgagtacgc cgacatgcag ctcatcgccg aggcctacga
    16441 cctcatccgt cgcggcactg gcaagactcc cgccgagatc tcggagatct tcaccgagtg
    16501 gaacaagggc gagctcgaga gctacctcat cgagatcacg gccgaggtgc tcaagcaggt
    16561 cgacgcgaag accggcaagc cgctcgttga cgtgatcacc gaccaggccg gctcgaaggg
    16621 caccggagtc tggaccgttc agaccgcgct cgacctgggc atcccggtgt ccggaatcgc
    16681 tgaggcggtg ttcgcccggg cgctgtcgtc gaaaccggcg cagcgggccg ccgcggcatc
    16741 cctgccgggt ccgtcggagg actcttggaa gcccggcgac cccgacgcct tcatcgaggg
    16801 cgtgcgtctc gcgctctacg cctcgaaggt catcgcctac agccaggggt tcgatgcgat
    16861 cgtcgccggc gccgagcagt acggctggga catcaagaag ggcgagatcg cgaagatctg
    16921 gcgcgggggc tgcatcatcc gggcgcgctt cctcaaccgc atcacggagg cgtacgccga
    16981 gaacccgggc ctcgtggcct tggtgacggc gcccttcttc gtcgacgtga tggccaaggc
    17041 acagaccgcg tggcgcaacg tcgtcgcgga tgccgcgcac gccggcatcc cgacgcccgc
    17101 gttctccagc tcgctcgcct actacgacgg gctgcgggcc gaccggctgc cggcagccct
    17161 cgtgcagggc cagcgcgact tcttcggcgc gcacacctac cagcgcgtcg acatgcccgg
    17221 cacattccac acgctgtggt cgggtgaccg caccgaggtg gagtcggccg gcagcagcca
    17281 ctagggcgcc gtcggctcct ccagcgcacg ctcgagagac gcggcctggt gtcgatggcc
    17341 cacggtctcg aagccgacga cgacaacggc cggtgacaac gtcacgatca cgaggcacac
    17401 cccgagcgac gctcctgccg ctgccagcgc gacggcgagc acgagcatga gcaccgtgcc
    17461 cgcgaacagc accgcgtgca gcgagtccca ctggcgcacg agataggcgt agagggcgaa
    17521 gttcactacc gagaacacca gcacgggtac ggcgatcgcg acaatcgcac cgacggttcc
    17581 gatgtcggta tggccctcga tcacgaacgc gacgacgtgc agccccgctc cggttgccgc
    17641 gatggccgca tagatgagga tgtggccgta cccccagacg aaaccccgtg accggtgacg
    17701 ggcgagcacg actcccgagg gcatgatgaa gtacgtccac cacaacccga aggtgatccc
    17761 gacccccgcg aacaccacga gggcggcctc gagactccag ccctgatcct cgaccagggc
    17821 ggcgacactg gccactgttc cgaacaccgc ctccccgaga gcgatgatcg cgagcaatcc
    17881 gtagcgctcc gcgatgtggt gcggatgcca cggcgtgccg cccaccttgc gctcggccac
    17941 caccggcccg cccagctcga tcgcgtacag cacgaacgcg aacatgacga aggtgcccac
    18001 cgataccggc agcagcgcga gcaccaccca gcccacctgt gcgatgagga tgtaggtcac
    18061 gtaggtcaga gcagttctgc ggtgggccgg gtcctggcgg gcgacccgta gccactgggc
    18121 aaccatcgcg atgcgcatga cgacatagcc cgcgacgata accccgttgt ccaggtggtc
    18181 gccctcgagc agcgaacgga acgccgcggg gatgccgagg gccagcacca cgacaccgat
    18241 catctgcacg aaggtggtga tgcggtagaa ccagtcgtcg gtgtcgaagg cc
//
LOCUS       DAOSOU010000015        17898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3174_length_17898_cov_5.872723, whole genome shotgun sequence.
ACCESSION   DAOSOU010000015 DAOSOU010000000
VERSION     DAOSOU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 17898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17898
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_17898_cov_5.872723"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLW15_01625"
     CDS             complement(<1..815)
                     /locus_tag="PLW15_01625"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOT33131.1"
                     /translation="MYRIFFTLVLSRIDPERAHRLAYAVIRALPALGVGALVRRFTRP
                     SAELSVSALGLSFDSPFGVAAGFDKEGTAIRGLGQLGFGHVEVGTITALAQPGNPKPR
                     LFRLVPDRAVINRMGFNNHGAAAAAPVIAKAVARRARPIVGVNIGKSRAVDVDDAVAD
                     YLESARLLAPHADYLVVNVSSPNTPGLRGLQELDKLAPLLTAVKKAAGDTPVLVKIAP
                     DLTDEQAGRIAALAVRELDGIIATNTTLSREGLETAASVVEAAGAGGLSGPPVA"
     gene            complement(835..1305)
                     /gene="nrdR"
                     /locus_tag="PLW15_01630"
     CDS             complement(835..1305)
                     /gene="nrdR"
                     /locus_tag="PLW15_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOT33132.1"
                     /translation="MFCPFCRHPDSRVIDSRTSDDGLSIRRRRQCPECGRRFSTTETA
                     SLSVIKRNGVVEPFSREKIVAGVRKACQGRPVTDTDLAVLAQRVEETIRATGAAQIEA
                     NDIGLAILTPLRELDEVAYLRFASVYQAFESLEDFESAITLLRVDHEQSETPTS"
     gene            complement(1324..2634)
                     /gene="hisD"
                     /locus_tag="PLW15_01635"
     CDS             complement(1324..2634)
                     /gene="hisD"
                     /locus_tag="PLW15_01635"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT33133.1"
                     /translation="MMQTIDIRTLQPSRSELARLVPRASTDVAAATESAAALIDDVRA
                     RGELALLEQAEKLDGVRPPHVRVHASEISEAVRHLVPQVRDALESAIERVRTATRAQV
                     PAGSVTVIAPGAEVIQRWQPVGRVGFYVPGGKAVYPSSVVMNVVPAQVAGVRSVALAS
                     PPQKAFGGAIHPTILGAAGLLGVDEIYAMGGAGAIGALAYGVPSIGLDPVDIITGPGN
                     IYVAAAKRLVRGVAGIDSEAGPTEILVIADASADPVLVAADLVSQAEHDELAAAVLVT
                     DSPELAGAVAAEVERIAASAHHGDRVRTALAGQQSAIVLVADLAAAAAFSNAYGPEHL
                     EIQTEVPGEVLDLIDNAGAIFLGAHSPVSLGDYLAGSNHVLPTGGQSRFSSGLGAYTF
                     LRPQQVIQYDRPALEAVSRAIVDLSTAEDLPAHGAAVTARFTET"
     gene            2704..3210
                     /locus_tag="PLW15_01640"
     CDS             2704..3210
                     /locus_tag="PLW15_01640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT33134.1"
                     /translation="MSDHTPEPEAGREAFIQAFRRHAAGVAAITSLAPDGRPVGFTAT
                     SLASLAATPPLATFNMARISTAWPAMTVGNRVVIHMLGPRTRHLAQRMAGDHDVRFEG
                     EHWHPGPDGLPLLDGVTAWMIGRIIAVHPVHENAVIVVEVVDGGLGEPDEALLYHERQ
                     YLRPGPTA"
     gene            complement(3207..6725)
                     /gene="dnaE"
                     /locus_tag="PLW15_01645"
     CDS             complement(3207..6725)
                     /gene="dnaE"
                     /locus_tag="PLW15_01645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT33135.1"
                     /translation="MASSDSFVHLHVHSEYSMLDGAARVGELMKAVSTEGMPAVAVTD
                     HGNMFGAYDFWKQATANGVKPIIGTEAYITPGTHRSDKSRVRWGSSQQGQDDVGGSGS
                     YTHMTLLSENNEGMHNLFRLSSLASIEGYYFKPRMDRELLSRYAPGLIATTGCVGGEV
                     QTRLRLGQYEEAKKAAAEFQDIFGKGNFFAEIMDHGIDIEKRTMRDLIRLAKELDLPL
                     VATNDLHYTHAHDATAHAALLCVQSASTLDDPNRFKFDSEEFYLKSAAQMRELFRDYP
                     EACDNTLLIAERCEVNFEKRDLMPRFPVPDGETEASWFEKEVATGMRRRFPQGPSEAH
                     LAQAKYEVDVITTMGFPGYFLVVADFIAWAKSQGIRVGPGRGSAAGSMASYAMGITEL
                     DPLAHGLIFERFLNPERVSMPDVDVDFDDRRRPEVIRYVTEKYGDDRVAQIVTYGTIK
                     AKQALKDSARVLGMPYSVGEKLTKAMPPAIMGKDISLGDVLNKEAERYKEAADLRALI
                     ETDPESARVFETARGIENLKRQWGVHAAGVLMSAEPLIDVLPIMRREDDGAIITQFDQ
                     PPLEDLGLLKMDFLGLRNLTVIEDALRMIEANTGAPLKIEELDLDADQATYDLLARGE
                     TLGVFQLDGGPMRALLKQLKPTNFEDISAVIALYRPGPMGMNSHTKYALRKNGLEQVD
                     AIHPELEEPLRDILGTTFGLIVYQEQVMSVAQKVAGFSLGQADVLRRAMGKKKKEELD
                     KQYAAFESGMVGNGYSTGAVKVLWETLMPFADYAFNKAHSAGYGVLSYWPAYLKANHP
                     AEYMAALLTSVGDSKDKLGMYLSECRRMGIKVLAPDVNESSADFTATGTDVRFGLGAV
                     RNVGFAVVELIARARAEKGAFTSFHDFLRKVPIQVANKRTVESLIKAGAFDSLGATRR
                     ALLEIHEDAVDSAVSLKRNEANGQVGFDFDSLWDEPEAVDHIPERPEWSKRDKLAFER
                     EMLGLYVSDHPLAGLETQLAKHQTASITEILGGDVAGDGEHVTIAGLVTSVQHRVARN
                     SGNQYGIVTVEDFAGEISVMFLGKTYQEFSPGLTNDSIVVIRGRVSVRDDGMNLHAVS
                     MFTPDTGPSLGSGPIVISVPEHRATTDIVSALNDVLIRHAGDNEVRLKLLKGTSARVF
                     EIPYPVTVSADLYGELKTLLGPNCLS"
     gene            complement(6797..7717)
                     /locus_tag="PLW15_01650"
     CDS             complement(6797..7717)
                     /locus_tag="PLW15_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT33136.1"
                     /translation="MESRSLFVPDGLAGDRVDAAIAKLLGFSRTFAAEVAEAGGVELD
                     GVVLGKSDRLTAGGWLSVTWTPKTEPSIIPVVVADLRIVHDDDDIVVIDKPPGVAAHP
                     SLGWDGPTVLGALAGAGYRIATSGAAERAGIVHRLDVGTSGLMVVAKSESAYSALKRA
                     FHDREVRKIYHAVVQGHPDPLAGTIDAPIGRHPGASWKFAITADGKPSVTHYETLEAF
                     PSAALLEIELETGRTHQIRVHMAAQRHPCVGDAMYGADPSISSRLGLTRQWLHAFRLG
                     FEHPATGEFVEFSSDYPPDLQHALEVLRGD"
     gene            complement(7723..8238)
                     /gene="lspA"
                     /locus_tag="PLW15_01655"
     CDS             complement(7723..8238)
                     /gene="lspA"
                     /locus_tag="PLW15_01655"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT33137.1"
                     /translation="MLAAVAVLVYVFDRVSKYLVTQNLTLGEPVEVLGPLLQFHYVEN
                     PGAAFSLGAGSTWIFAIIAAAVTVFIVIFSRRIRSVGWAVLFGLLLGGTLGNLTDRLT
                     REPGFGRGYVVDFLQIWGFPAIFNIADIGIVSSMGLFIILTLRGVGLDGKRTPRPAKT
                     GAADQSADDTP"
     gene            complement(8288..9031)
                     /locus_tag="PLW15_01660"
     CDS             complement(8288..9031)
                     /locus_tag="PLW15_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT33138.1"
                     /translation="MALTPEDVVNKRFQPTKFREGYDQDEVDDFLDEVVVELRRLNQE
                     NEELRQRLIASDARMNELQRTADQGGFQQQAPAMPAAAPVQQPAFAQPVPDAAGMDPS
                     NTNNLLQLARRLHEEHVREGVEKRDALIAEGHATAARVVAEAEARQRGELTSLEQERQ
                     QLERRIEELRNFEREYRQKLKGYIEGQLRELDATNELTATPAPAAAAQYAAPAPSYPA
                     QPAQQQYPDYQAQQPAQTPPNYTGGFAGN"
     gene            complement(9140..9439)
                     /locus_tag="PLW15_01665"
     CDS             complement(9140..9439)
                     /locus_tag="PLW15_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOT33139.1"
                     /translation="MTLVSIVATIVYVGLTIFFVLMWARFILDLVRVFARRWRPRGVG
                     LVLAEFVYVVTDPPIKLVRRVVPPLRVGGAALDFAWSIVMLAVIILISVALGFVN"
     gene            complement(9480..9944)
                     /gene="sepF"
                     /locus_tag="PLW15_01670"
     CDS             complement(9480..9944)
                     /gene="sepF"
                     /locus_tag="PLW15_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOT33140.1"
                     /translation="MANPLRRTMVYLGLADEDYEYDHQSGPVAPVAPVAHNSAPAPSR
                     GSVTPLRRASNHRGAAAEMNEILTVHPRQYKDAQLIAENFREGIPVIINLSQMNETDA
                     RRLIDFACGLSEGLYGKIERVTSKVFLLSPAHVAVSGDHAEAESDVDASFFA"
     gene            complement(9982..10647)
                     /locus_tag="PLW15_01675"
     CDS             complement(9982..10647)
                     /locus_tag="PLW15_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT33141.1"
                     /translation="MPDLEARLASVTAEVAQAARDAGRNPAEITTIVVTKFHPAALVR
                     ELADLGVSDFGESRHQEAQPKIAELADLAATWHFIGQVQSKKARQVRSYVDVIHSVDR
                     PSLVDALAGGPLDVFIQVNLTDDPARGGAQPDDLASLSERVASTEGLKLRGLMAVAPL
                     DSEPRREFARVRTLSERLRAAHPEASGLSMGMSGDFREAILEGATHLRIGTAITGNRL
                     LHG"
     gene            complement(10650..11783)
                     /gene="ftsZ"
                     /locus_tag="PLW15_01680"
                     /pseudo
     CDS             complement(10650..11783)
                     /gene="ftsZ"
                     /locus_tag="PLW15_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            complement(11954..12601)
                     /locus_tag="PLW15_01685"
     CDS             complement(11954..12601)
                     /locus_tag="PLW15_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOT33142.1"
                     /translation="MASLVIIILAAIFSPILALRQIQIDGTSRVSADEVHSAVDGQLG
                     TPLALIDYGKLTGELAAFPLIRSFVTEVVPPDTLLIHIVERQPVGAILTGGTFHLVDP
                     AGVTVQESADRLPGVPVIDIGGAPTSGVVFDSVVEVLLSLPPELLAQVDSVSATTQDN
                     VSLVLTGVGQRVTWGSADQSQRKAALLAALIAQTDPASAGEFDVSAPNNGVFRPS"
     gene            complement(12922..14352)
                     /gene="murC"
                     /locus_tag="PLW15_01690"
     CDS             complement(12922..14352)
                     /gene="murC"
                     /locus_tag="PLW15_01690"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT33143.1"
                     /translation="MIKPDLTMPLPGELGAVHFVGIGGSGMSGIARLFLAAGHTVSGS
                     DRSANHNTDDLTALGATVSIGHSADNPGLSTADALVYTGALWPDNPEYLTAVARGIPV
                     LHRSQALAWLINRQRLVAVAGAHGKTTSTGMIITALLELGADPSFVNGGVIQSLGLSA
                     SFGSGELFVVEADESDGSFLLYDTSVALITNVDADHLDHYGTHDEFDAAFVAFADRAS
                     ELVVISSDDPGAVRVTRSLSHSKIMTFGEDAAADVRVHDIVTDGPVAFELTYKSVSYP
                     ARLLVPGKHNALNAAGAFAVLVGLGFEPADALAGIEKFAGTERRFELHGVVRGVSVYD
                     DYAHHPTEVAAALSAARSVVGEGRIIAVHQPHLYSRTQAMAGEFAETYERLADHTIVL
                     DVFGAREDPVPGVTGALVSGRFADASNVDFLPDWQQASDRAAELARDGDIIMTLSCGD
                     VYRIVPQLLASLASTPERAGGVATPA"
     gene            complement(14365..15462)
                     /locus_tag="PLW15_01695"
     CDS             complement(14365..15462)
                     /locus_tag="PLW15_01695"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOT33144.1"
                     /translation="MTTYLLAGGGTAGHVNPLLAVADRLRERDPASTILVLGTREGLE
                     ARLVPERGYELVTIERLPFPRRPGRAALTFPGRLRAAVAEVRSIITSRGIDVVVGFGG
                     YAAAPAYLAARDESTPLVVHEANARPGIANRLGARFTPFVGVAFEGTRIPHATWVGMP
                     LRPEIERLDRFAARREGIRLFDLVEDRPTLLVTGGSSGAQRINETVLASAPTILGVGW
                     QVLHVTGEFREAVDDPRLPGYRVLKYCDRMDLALAVADLSLSRAGTSTVAEVTALGVP
                     SVFVPYAAGNGEQRLNARGAVAAGGAIIVADADFTPQWVAAELVPLLRNRALIADMAV
                     RIATVGVIDGTDRMVALIDAALAAAPSLGRR"
     gene            complement(15459..16664)
                     /gene="ftsW"
                     /locus_tag="PLW15_01700"
     CDS             complement(15459..16664)
                     /gene="ftsW"
                     /locus_tag="PLW15_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOT33145.1"
                     /translation="MIPVRRIFAIENRNYFLLLGTTLFLVAFGLVMVLSSSSIESSVD
                     GEGFFGRFIRQGFSAILGIPLMLVASRMPPRFWKRWAWPALLVGGALQLIVFIPGLGL
                     EHGGNQNWISIGGFNMQPSEFVKIALIVWIAWVLSTKEELLGDWRHVALPIAPVAGVA
                     ILFVLIGKDLGTASIIVMIVLGCLYFAGVRLKFLAVALAGVSLLVIIMALATPSRTER
                     ISVWLNGCTAADYSNLCWQVQHASWALAAGGLFGSGLGNSKAKWNWLPEADNDFIFAI
                     IGEELGLIGAIVVLVLFIVLAIAFVRVLRATRDSFARITTGGVMVWVIGQALVNIAVV
                     LGLLPVLGVPLPLISAGGSALIAILLGIGVVLSFARQTDRESALDQGVAQTPAQRSRL
                     LASNRAARR"
     gene            complement(16900..>17898)
                     /gene="murD"
                     /locus_tag="PLW15_01705"
     CDS             complement(16900..>17898)
                     /gene="murD"
                     /locus_tag="PLW15_01705"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT33146.1"
                     /translation="TSWYADWSSLRVAVLGLGATGFAAADTLTELRCAVLVVAAEADE
                     ERIDLLSVIGARYNPQPDREVIPPDLASFDPELIVVSPGYAPDHPLVAWAREGGIPIW
                     GDVELAWRLRDKVAPPAHWNLIAGSSGVEMTSLLAAHLLVGGGRRYVVAGSVGMLALD
                     AVRDPVGFDGLIVALSPAQLQWITRSGPGALRPEVSVCLTVDPASDLPQEVFGRVYEN
                     TAIACVYNQADEATMHLVEDADVQEGCRAIGFGLGAPGRSDLGMVGDIVVDRAFLDER
                     HSTALELTTHGELAEADLATAELLPQVLAACAVARAHGVSPAEVRTALATFMPTTG"
BASE COUNT         3029 a         5810 c         6132 g         2927 t
ORIGIN      
        1 gcgacgggcg gtccggacag tcccccggcg ccggcggcct cgacgacgga tgccgcggtc
       61 tcaagtccct ccctcgacag cgtcgtattg gtcgcaatga tgccgtcgag ctcacgaacg
      121 gccagcgccg caatgcggcc cgcctgctcg tcggtgagat cgggggcaat cttcaccagc
      181 acgggtgtat cgccggcggc cttcttgacc gccgtgagca gaggggcgag cttgtcgagc
      241 tcctggagtc cgcgcagtcc cggggtgttc ggagaactga cgttgaccac gagatagtcg
      301 gcgtgaggcg cgagaagtcg ggcgctctcg aggtagtcgg cgaccgcgtc atccacgtcg
      361 acagcacgag acttgccgat gttcacgccg acgatcggcc gggctcggcg cgcgactgcc
      421 ttggcgatca cgggggctgc agcggccgcc ccgtggttgt tgaaccccat gcggttgatc
      481 acggcccggt cgggcacgag tcggaagagt ctcggcttcg gattgcccgg ttgggcgagc
      541 gcggtgatcg tgccgacctc gacatgaccg aagccgagtt gaccgagccc gcggatcgcg
      601 gtgccctcct tgtcgaagcc cgccgcgacg ccgaagggcg agtcgaagct caggccgagc
      661 gccgagaccg agagctctgc ggacgggcgt gtgaaccggc gcaccagcgc tccgacaccg
      721 agcgccggaa gggcgcgaat caccgcatag gcgagccggt gtgcgcgctc gggatcgatc
      781 cgcgagagaa ccagggtgaa gaagatccga tacatgctga ggacaggcta tcggtcagct
      841 ggttggggtc tccgactgtt cgtggtcgac gcgcaacagg gtgatcgctg actcgaagtc
      901 ctccagcgac tcgaaggcct ggtagacgct ggcgaaccgc aggtaggcga cctcgtcgag
      961 ttcgcgcagc ggtgtgagaa tcgccagccc gatgtcgttg gcctcgatct gggcggcacc
     1021 ggtggcgcgg atggtctcct cgacgcgctg cgcgagaacc gcgagatccg tgtcggtcac
     1081 cgggcgtccc tggcaggcct tgcgcacccc agcgacgatc ttctcccggc tgaagggctc
     1141 gaccacaccg ttgcgcttga tcacgctgag gctggcggtc tccgtggtgg agaagcggcg
     1201 accgcactcg gggcactgac ggcggcgacg gatcgacagc ccgtcgtcac tcgtgcggga
     1261 gtcgatgacg cgactgtcgg ggtgacggca gaaggggcag aacatggtgc cccaaggata
     1321 cgcctaggtc tcggtgaacc tggcggtgac cgccgcaccg tgcgcaggca ggtcttcggc
     1381 ggtgctcagg tcgacgatcg ctcgcgagac cgcctcgagg gccggtcggt cgtactggat
     1441 gacctgctgg ggtctcagga acgtgtaggc gccgaggccc gaggagaacc gggactgccc
     1501 gccggtgggc agcacgtgat tggagcccgc gaggtagtcg cccaggctca ccggggagtg
     1561 cgcaccgagg aagatcgctc cggcgttgtc gatgaggtcg agaacctccc cgggaacctc
     1621 ggtctggatc tcgaggtgct cgggtccgta ggcgttgctg aatgcggctg cggccgcgag
     1681 gtcggctacc agcacgatcg ccgactgctg cccggcaagc gcggtgcgca cccggtctcc
     1741 atggtgagcg gatgccgcga tccgctcgac ctcggcggcg accgcgcccg ccagctcggg
     1801 tgaatcggtg accagtaccg cggcggcaag ctcgtcgtgc tcggcctggc tcacgagatc
     1861 ggcagcgacg agtacggggt cggcggaggc gtcggcgatg acgaggatct cggtggggcc
     1921 cgcctcggag tcgataccgg cgacgccgcg caccagtcgc ttggcggcgg cgacgtagat
     1981 gttgccgggg ccagtgatga tgtcaacggg atcgaggccg atggacggca caccgtaggc
     2041 gagggcgccg atggctcctg ccccgcccat cgcgtagatc tcatcgacgc cgagcagccc
     2101 ggccgcgccg aggatggtgg ggtggatggc accgccgaat gccttctgcg gtggggaggc
     2161 gagcgcgacg gagcgaaccc cggcgacctg tgcgggcacg acgttcatga cgacgctcga
     2221 gggatagacg gccttgccgc cgggaacata gaaccccacg cggccgaccg gctgccagcg
     2281 ttggatgacc tcggcgccgg gagcaatcac cgtgaccgat ccggcgggca cctgcgcccg
     2341 ggtcgcggta cgcacccgtt cgatcgccga ctcgagggcg tcgcgcacct gcggaacgag
     2401 gtggcgcacg gcctcggaga tctccgatgc gtgcacgcga acatgcgggg gtcgaacgcc
     2461 gtccagcttc tcggcctgct cgagcagggc cagctcgccg cgggctcgaa catcgtcgat
     2521 cagagcggca gcagactcgg ttgcggcagc cacatcggtg ctcgcccggg gaacgagtcg
     2581 ggcgagttcg ctgcggctcg gctggagggt gcggatgtcg atcgtctgca tcatggcctg
     2641 tcaatgctag ttcgtggccg agtgggaata gcgcacggcc atgcgtgctt gactcgtgat
     2701 gtcatgagcg atcacacacc cgagcccgaa gccggtcgcg aagccttcat ccaggcgttc
     2761 cgcaggcatg ccgccggtgt cgccgcaatc acatctctcg cccctgacgg gcgaccggtg
     2821 ggattcaccg cgacatcact cgcctccctc gctgccactc ccccgctggc gaccttcaac
     2881 atggctcgca tctcgaccgc gtggcctgcg atgaccgtgg gcaatcgggt ggtcatccac
     2941 atgctcggtc cgcgcacccg gcaccttgcc cagcggatgg caggagacca cgacgtgcgc
     3001 ttcgagggcg agcactggca tccgggcccc gacggactcc cgctgctcga tggcgtcact
     3061 gcgtggatga tcggccgcat catcgccgtg caccccgtgc acgagaacgc cgtgatcgtc
     3121 gtcgaggtcg tggacggcgg actgggcgaa cccgacgagg cactgctgta tcacgagcgg
     3181 cagtacctgc gccccggacc aaccgcctag ctgaggcagt tgggaccgag cagggtcttc
     3241 agttctccgt agagatcggc cgagacggtg accgggtagg gaatctcgaa gactctcgcg
     3301 ctggtgccct tgagcagttt gagccgcact tcgttgtctc ccgcgtgcct gatcagcacg
     3361 tcgttgaggg cggagacgat gtcggtcgtt gcacgatgct cgggaaccga gatcacgatc
     3421 ggacccgatc ccagactcgg cccggtgtca ggcgtgaaca tgctcacggc gtgcaggttc
     3481 atgccgtcgt ctcgcacact cactcgcccg cggatcacga cgatggagtc gttggtcagg
     3541 ccgggtgaga actcctggta tgtcttgccc aggaacatga cgctgatctc tcctgcgaag
     3601 tcctcgacgg tcacgattcc gtactggttg ccgctgttgc gggccacccg gtgctgcacg
     3661 ctcgtgacca gcccggcgat cgtgacatgc tcaccgtcgc cagcaacgtc accgccgagg
     3721 atctcggtga tggatgccgt ctggtgcttc gcgagctgag tctcgagacc cgcgaggggg
     3781 tgatcggaga cgtagagtcc gagcatctcc cgctcgaagg ccagtttgtc gcgcttggac
     3841 cattcgggtc gctccgggat gtggtccacc gcctccggtt catcccagag gctgtcgaag
     3901 tcgaatccga cctggccgtt ggcttcgttg cgcttgaggc tcaccgcgga gtcgaccgcg
     3961 tcttcgtgga tctccagtag cgcgcgccga gtggctccga gagagtcgaa cgcgccggcc
     4021 ttgatgagcg attcgaccgt gcgcttgttg gccacctgga tcggaacctt gcgcaggaag
     4081 tcgtggaaac tggtgaaggc acccttctcc gcacgcgccc gcgcgatgag ttcgacgacg
     4141 gcgaatccga cgttgcgcac ggcaccgagg ccgaagcgca cgtcggtgcc cgtcgcggtg
     4201 aagtcggcac tcgactcgtt cacgtcgggc gcgagcacct tgatgcccat gcgacggcac
     4261 tcgctgaggt acatcccgag cttgtccttg gagtcgccga cgctcgtgag cagggccgcc
     4321 atgtactcgg ccggatgatt cgccttgagg tacgccggcc agtagctcag cacgccgtag
     4381 ccggcgctgt gagccttgtt gaaggcgtag tcggcgaagg gcatcagggt ttcccagagc
     4441 accttgacgg cacccgtgct gtacccgttg ccgaccatgc ccgactcgaa ggcggcgtac
     4501 tgcttgtcga gctcctcctt cttcttcttg cccatggccc gccggagtac gtcggcctgg
     4561 ccgagtgaga atcccgcgac cttctgggcg acgctcatga cctgctcctg gtacacgatc
     4621 agaccgaagg tggtgccgag gatgtcgcgc aggggctcct cgagttcggg atggatcgca
     4681 tccacctgct cgagtccgtt cttgcgcagg gcgtacttgg tgtgcgagtt catgcccatc
     4741 ggcccgggac ggtagagcgc gatgacggcg gagatgtcct cgaagttggt gggcttgagc
     4801 tgcttgagga gggcccgcat cggcccgcca tccagctgga acaccccgag cgtctcgccc
     4861 cgcgccaaca ggtcgtaggt cgcctggtcg gcgtccaggt cgagttcctc gatcttcagg
     4921 ggggcacccg tgttcgcttc gatcatgcgc agggcatcct cgatgacggt caggttgcgc
     4981 aagccgagga agtccatctt cagcagaccg aggtcttcga gcggcggctg gtcgaactgg
     5041 gtgatgatgg cgccgtcatc ctcacgacgc atgatcggca gcacgtcgat caggggctcg
     5101 gccgacatga gcacgccggc cgcgtgcacg ccccattggc gcttgaggtt ctcgatgccc
     5161 cgggcagtct cgaacacgcg agcggactcg ggatcagtct cgatgagcgc ccgaagatcg
     5221 gccgcctcct tgtagcgctc cgcctccttg ttgagcacgt cgccgagtga gatgtccttg
     5281 cccatgatgg ccgggggcat cgccttcgtg agcttctccc ccaccgagta cggcataccc
     5341 agtactcgcg cagagtcctt gagggcctgc ttggccttga tcgtgccgta ggtgacgatc
     5401 tgcgcgaccc ggtcgtcacc gtacttctcg gtgacgtacc tgatgacttc gggacgtcgg
     5461 cggtcgtcga agtccacatc gacatcgggc atggacacgc gctccggatt gagaaagcgc
     5521 tcgaagatga ggccgtgtgc cagcgggtcg agctcggtga tccccatggc gtacgaggcc
     5581 atcgatccgg cggccgagcc acgccccggc cccacgcgaa tgccctggct cttcgcccag
     5641 gcgatgaagt cagccaccac caggaagtag cccgggaagc ccatcgtcgt gatgacgtcg
     5701 acctcgtact tggcctgcgc cagatgggct tcgctcggtc cctgcgggaa gcggcgtcgc
     5761 atccccgtcg ccacctcctt ctcgaaccag gatgcctcgg tctccccgtc gggcaccggg
     5821 aaccggggca tcaggtcccg cttctcgaag ttgacctcgc agcgctccgc gatcagcagg
     5881 gtgttgtcgc aggcctcggg atagtcgcgg aacagctcgc gcatctgcgc ggctgacttc
     5941 aggtagaact cctcggagtc gaacttgaag cggttggggt cgtcgagggt cgacgctgac
     6001 tgcacgcaca gcagcgcggc gtgagcggtc gcgtcgtgcg cgtgcgtgta gtgaaggtca
     6061 ttggtcgcca cgagaggcag gtcgagttct ttcgccagcc ggatgaggtc acgcatcgtg
     6121 cgcttctcga tgtcgatgcc gtggtccatg atctcggcga agaagttgcc cttgccgaag
     6181 atgtcctgga actccgctgc ggccttcttc gcctcctcgt actggccgag gcgcaggcgc
     6241 gtctgaacct cgccaccgac gcatcccgtg gtcgcgatga gacccggtgc gtagcggctg
     6301 aggagctcgc ggtccatgcg cggcttgaag tagtagccct cgatcgaggc cagcgaggag
     6361 aggcggaaca ggttgtgcat gccctcgttg ttctccgaga gcagggtcat gtgggtgtac
     6421 gaacccgagc cgccgacgtc atcctggcct tgctgactgg agccccagcg cacccgcgac
     6481 ttgtcgctgc ggtgggttcc cggcgtgatg tacgcctcgg tgccgatgat cggcttcacg
     6541 ccgttggctg tggcctgctt ccagaagtcg taggcgccga acatgttgcc gtggtcggtg
     6601 acggcgactg cgggcatccc ctcggtcgac accgccttca tgagctcgcc cacccgcgcg
     6661 gcaccgtcga gcatggagta ctcgctgtgc acgtggaggt gcacgaatga gtctgaggat
     6721 gccactgttg tctcttcact agcggggtgg aataccactg taggcgtgtg tcagaccggg
     6781 gtcatccccg acgcgcctag tcgccgcgaa gcacctcaag tgcgtgttga aggtcgggcg
     6841 gatagtcgct cgagaactcc acgaactcgc cggtcgccgg atgctcgaac cccagccgga
     6901 aggcgtgcag ccactggcgt gtgaggccga gtcgagagga gatcgaagga tcagcgccgt
     6961 acatggcgtc tcccacgcac ggatgccgct gcgccgccat gtgcacccgg atctggtgag
     7021 tgcgacccgt ctccaactcg atctcgagca gcgccgccga ggggaacgcc tcgagcgtct
     7081 cgtagtgcgt cacactcggc ttgccgtcgg cggtgatcgc gaacttccag ctcgcaccgg
     7141 ggtgtcgacc gatgggagca tcgatcgtgc ccgcgagggg gtcggggtgg ccctgaacca
     7201 cggcgtggta gatcttgcgc acctcgcggt catggaaggc ccgcttgagt gccgagtagg
     7261 cggactcgct cttggccacg accatgagcc cgctcgtgcc cacgtcgagt ctgtgcacga
     7321 ttcccgcgcg ttcggcggcg ccggatgtcg cgatccggta gcccgcgccg gcgagggcac
     7381 cgagcacggt cggcccatcc caccccaggc tcgggtgcgc cgcgactccc ggtggcttgt
     7441 cgatgaccac gatgtcgtca tcatcgtgaa cgatccggag atcggcgacg accacgggaa
     7501 tgatcgacgg ctcggtcttg ggcgtccacg tgacgctcag ccagcctccg gcggtgagcc
     7561 gatccgactt gccgagcacg accccatcga gctcgactcc cccggcttcg gcgacctcgg
     7621 ccgcgaaggt tcgcgagaat ccgaggagct tggcgatcgc ggcatccact cggtcacccg
     7681 ccaggccgtc gggaacgaac aggctcctgg actccatcgg gatcagggag tgtcgtcggc
     7741 ggactgatcc gcggcgccgg tcttggccgg tctcggagtg cgcttgccgt cgagtccgac
     7801 cccgcgaagg gtcaggatga tgaacaggcc catcgacgag acgattccga tgtcggcgat
     7861 gttgaagatc gcggggaatc cccagatctg caggaagtcc acgacgtagc cacggccgaa
     7921 gccgggctcg cgggtcaaac ggtcggtgag attgccgagc gttccgccga gcaggagtcc
     7981 gaacagcacc gcccagccga ccgaccggat gcgacgggag aagatcacga tgaagacggt
     8041 gaccgcggcc gcgatgatgg cgaagatcca cgtgctgcct gcgccgaggg agaacgcagc
     8101 acccgggttc tcgacgtagt ggaactgcag gagaggaccg agaacctcga cgggctcacc
     8161 gagggtgaga ttctgggtga cgaggtactt gctgacgcga tcgaatacgt agacgagcac
     8221 tgcgacggcc gcgagcaaag ccaaagcccg gggactgacc ccgggctttg cgaccgtctt
     8281 cggcccgtca gtttccggca aagccgccgg tgtagttcgg aggcgtctgc gcgggctgct
     8341 gagcctggta gtccgggtac tgctgctgcg ccggctgtgc cgggtagctc ggcgcgggag
     8401 ccgcgtactg ggctgctgcg gcaggggcgg gcgttgctgt caactcgttg gttgcgtcga
     8461 gctcgcggag ctgaccctcg atgtatccct tgagcttctg gcggtactcg cgctcgaagt
     8521 tgcggagctc ctcgatgcga cgctcgagct gctggcgctc ctgctcgagc gaggtgagct
     8581 cgccacgctg gcgcgcctct gcctcggcaa cgacacgggc cgcggtcgcg tgaccctcgg
     8641 cgatgagcgc gtcacgcttc tcgacaccct cgcgcacgtg ctcctcgtgg aggcgacgcg
     8701 cgagctggag aaggttgttg gtgttgctcg ggtccatgcc agcggcgtcg gggacgggct
     8761 gagcgaacgc gggctgctgc accggagccg cagccggcat cgcgggagcc tgctgctgga
     8821 agccaccctg gtcagcggtg cgctgcagct cgttcatgcg cgcgtcgctg gcgatgaggc
     8881 gctggcgcag ctcctcgttc tcctggttga ggcgacgcag ctcgacgaca acctcatcga
     8941 ggaagtcatc gacctcgtcc tggtcgtatc cctcgcggaa cttcgtcggc tggaaccgct
     9001 tgttgaccac gtcttctgga gtcagagcca tcgccgtcac ctcacactct ttctttcagc
     9061 acgcttgtac agcgtcacag tttacccatc gacggtgcgt gtgtaccgtc gtcagattct
     9121 ccacagtaca gcgctcgggc tagttcacga atcccaaggc gacagaaatc aggatgatga
     9181 cagccaacat cacgatgctc cacgcgaaat cgagcgcggc tccgccgacc cgcagcgggg
     9241 ggacgaccct acgcacgagc ttgatcggcg ggtcggtcac gacatagacg aactcggcaa
     9301 gaaccagtcc aaccccgcgc ggacgccagc gtcgggcgaa cacccgaacg aggtcgagga
     9361 tgaagcgtgc ccacatgagc acgaagaata tggtcaggcc gacgtagacg atcgtcgcga
     9421 caatggagac aagagtcacc tcagaagact acgtcgctgc tggacgaacg gcgcgcagct
     9481 caggcgaaga atgaggcgtc gacgtcgctt tcggcctcgg cgtgatcgcc gctcaccgcg
     9541 acatgcgccg gcgacagcag gaacaccttg ctcgtcacac gctcgatctt gccgtacagc
     9601 ccctcggaga gtccgcaggc gaagtcgatg agcctgcggg catccgtctc attcatctgg
     9661 gaaagattga tgatgacggg gatgccctcg cggaagttct cggcgatcag ctgggcatcc
     9721 ttgtactggc gcgggtgcac agtaaggatc tcgttcatct ccgccgcggc gccccggtgg
     9781 ttggaggcac gacgcaaagg cgtcaccgat ccgcgcgacg gtgcgggagc cgagttgtgg
     9841 gccaccggcg cgacgggcgc gacgggtccc gactggtggt cgtactcgta gtcctcgtcg
     9901 gcgaggccga ggtagaccat tgtcctgcgc agtgggttgg ccatgtcaat ccttacggct
     9961 agtgaagctt atggttcgag attaaccatg aagtagacgg tttcccgtga ttgccgtgcc
    10021 aatgcgcagg tgtgtcgcgc cctccaatat cgcttcgcgg aagtcacccg acattcccat
    10081 cgagagcccg gatgcctcgg ggtgggccgc gcggaggcgc tccgacaggg tgcgcacgcg
    10141 agcgaactcg cgcctcggct cgctgtcgag gggcgccacg gccatgaggc ctcgcaactt
    10201 gagtccctcg gtggatgcca ctcgctcaga gagcgacgcg agatcgtcgg gctgggctcc
    10261 cccgcgcgct ggatcatcgg tcaggttgac ttgtatgaag acgtcgagcg ggccacccgc
    10321 cagcgcgtcg accaaggacg gtcgatccac ggagtggatg acatcgacat aggaccgcac
    10381 ctggcgagcc ttcttcgatt gcacctggcc gatgaagtgc caggtcgcgg cgagatcggc
    10441 gagctccgcg atcttgggct gggcctcctg atggcgactc tctccgaagt cactaacgcc
    10501 cagatcggca agctcccgca ccagtgccgc cggatggaac ttcgtcacga cgatcgtcgt
    10561 gatctccgcc gggttccggc cggcgtcgcg tgccgcctgc gccacctcgg cggtgaccga
    10621 ggccaggcgc gcctcgaggt cgggcaccgc tacttgagga agtcgggaat gtccaggtcg
    10681 ccctcgtcgt cgtcgaagct tcgatcgatg ggggcggtga ccagactcgg ttccggggcg
    10741 cgcaccggtt catcggccga gggcacgacc ggcgcggaag actcgggcac tacgtagttc
    10801 gtgcggcggg catcccgtgt cttggtggtc ggctcgccgc catcgaagcc cgccgcaatc
    10861 acggtgacgc gaacctcgtc gccgagagtg tcgtcgatca ctgcgccgaa gatgatgttc
    10921 gcctcgggat gcacggcctc ctgcaccagg cgcgcggcat cgttgatctc gaagatgccc
    10981 aggttggagc cgccctggat cgacagcagc actccgtgtg cgccgtcgat ggatgcctcg
    11041 agcagcgggg atgcgacggc gagctccgcc gccttgatgg cgcggtcggc tcccctcgcc
    11101 gacccgatgc ccatgagcgc tgagcccgca ccctgcatga ccgacttcac gtcggcgaag
    11161 tcgaggttga tcagtccggg tgtggtgatg agatcggtga tgccctgcac accggcgagc
    11221 agcacctgat ctgccgtggc gaaggcttcg agcatgctga tgccccggtc gctgatctcg
    11281 aggagccggt cgttcggaac gacgatgagg gtgtcgacct cgttcttgag gctcgcgact
    11341 ccgacttcgg cctgggcaga acggcgcttt ccctcgaatc cgaagggctt cgtgacgaca
    11401 ccgattgtga gcgccccgat cgacttggcg atgcgagcca cgacgggggc gccaccggtg
    11461 ccggttccgc cgccctctcc ggcggtgacg aacaccatgt ctgcgccagc gagtgcctct
    11521 tcgatctcct cggcgtgatc ctcggcggcg cgacggccga cttccgggtc ggcgccagcg
    11581 ccgagtccgc gggtgagctc gcgaccgaca tccagcttca catcggcgtc actcatcagc
    11641 agggcctggg cgtcggtgtt gatcgcaatg aactcgacgc ctcgcaggcc gagctcgatc
    11701 atgcggttga cggcgttgac accgccaccg ccgatgccca ctaccttgat caccgcgagg
    11761 tagttctggt ttgacgtcac ttccggcctc cgctgcctgc tctgggagct ttactgtata
    11821 aaccttcaac ctctacttga agattgaagt tttactcagt atgcttctcc tccgttgaga
    11881 gtaggtcggc tcgccgcacc gcgccctgaa tgggttcagc gtgtcgggaa acctctgtgc
    11941 gacctgagtc agctcaggag ggccggaaca ccccattgtt cggcgcggac acatcgaact
    12001 cgcccgcgct ggccggatcc gtctgggcga tcagcgccgc cagaagcgcg gcctttctct
    12061 gcgactgatc ggcactgccc cacgtgaccc gctggcccac accggtcaga acgagcgaca
    12121 cgttgtcctg agtcgtcgcc gacaccgagt cgacctgtgc gagcaactcg ggaggaaggg
    12181 agagcagcac ctccaccacc gaatcgaaga ccacaccgga cgtcggcgcg ccaccgatat
    12241 cgatgacggg aacgccgggc agtcggtcag cggactcctg cacggtcacg ccggcgggat
    12301 cgacgagatg gaaggtaccg cccgtcagaa tcgctcccac cggctggcgt tcgacgatgt
    12361 ggatcaggag tgtgtcgggc ggcaccacct cggtcacgaa gctgcggatg agcggaaagg
    12421 cggcgagttc gcccgtgagc ttgccgtagt cgatgagtgc gagaggggtg ccgagctgcc
    12481 cgtcgacagc actgtggact tcatcggcgc tgacccgcga cgtgccgtcg atctggatct
    12541 gccgcagtgc gagaatcggc gagaagatcg ccgccaggat gatgatgacc agactcgcca
    12601 tcgtgcccag taccagtgca atcgcgacgc gtcggtttcg ggcacgtcgg gtgaaccgac
    12661 gcacctcggc acgctcgtac ttgcgacgcg cccgcgccgc actgcgaagc tctgcccgcg
    12721 ccgcggggtc gggtcggggc tgctgactcg ggcgcggcac cgtggcggcc gcgggtggtg
    12781 tcgccgaccc tgctggccgc gcgggcttga cctgagaagg gtgggtcgag cccgacgcgc
    12841 gcacccgcga cggctgggac ggcttacgtc ccggcggcgg cggttgcgac cgccccggcg
    12901 ggtcgaaacc ctcgggtcgt ttcatgcggg agttgcgacc ccgcccgccc tctcaggcgt
    12961 cgacgcgagg gaggcgagaa gctgcggcac aatccggtac acatcaccgc agctgagggt
    13021 catgatgatg tcgccgtcac gggcgagctc cgccgcccga tcggatgcct gctgccagtc
    13081 cggcaggaag tcgacgttgc tcgcgtcagc gaaacgcccg gagaccagcg cacccgtgac
    13141 ccccgggacc ggatcctcgc gggcgccgaa cacgtcgagc acgatcgtgt gatcggctag
    13201 gcgctcatag gtctcggcga actcaccagc catggcctgc gtgcgactgt agaggtgcgg
    13261 ttggtgcacg gcgatgatgc gcccctcccc cacgacactg cgcgccgcgc tcagcgcggc
    13321 cgcgacctcg gtggggtgat gggcgtaatc gtcgtagacg ctgacccctc gcacgacacc
    13381 gtgcagctcg aatcgccgct cggttcccgc gaacttctcg atgccagcga gggcgtcggc
    13441 tggctcgaac cctagtccca cgagcacggc gaaggcgccg gcagcgttca gggcgttgtg
    13501 cttgccggga accagaaggc gagccgggta cgagaccgac ttgtaggtga gctcgaaggc
    13561 caccgggccg tcggtgacga tgtcgtgcac cctgacgtcg gcggcggcat cctcaccgaa
    13621 ggtcatgatc ttcgagtggg agagcgaacg agtgacgcgc accgcgcccg ggtcgtcgct
    13681 ggagatcacg acaagctccg aagcgcgatc ggcgaacgcc acgaaagcgg catcgaactc
    13741 gtcgtgggtt ccgtagtggt ccaggtggtc tgcgtcgacg ttggtgatca gagccacgga
    13801 cgtgtcgtag agcaggaacg aaccgtcgga ttcgtcggcc tcgaccacga acaactcccc
    13861 cgacccgaac gacgcactga gccccagaga ctggatgacg cccccgttga cgaaactggg
    13921 atcggcgccc agttcgagca acgccgtgat gatcatccct gtggaggtgg tcttgccgtg
    13981 cgcgccggcc acggcgacga gcctttgccg attgatcagc caggcgagcg cctgagaacg
    14041 gtgcagcacc gggatgccgc gcgccacggc agtcaggtat tccgggttgt cgggccagag
    14101 cgcaccggtg tacaccagcg cgtcagccgt cgacaggccc gggttgtcgg cggagtgacc
    14161 gatggagacc gtcgcaccga gcgcggtgag gtcgtcagtg ttgtgattcg ccgaccgatc
    14221 ggatccactg acggtgtgac ccgcagccag gaagagacgg gcgatgccac tcatgcccga
    14281 acctccgatg cctacgaagt gcacggcacc gagttcgccg ggcaggggca tggtgaggtc
    14341 tggcttgatc atcgtgacac gattttaccg tcgccccagc gagggagccg ccgcgagggc
    14401 ggcgtcgatg agagcgacca tgcggtcggt gccgtcgatc actccgactg tggcgattcg
    14461 cacagccatg tcggcgatga gtgccctgtt acgcagaagc ggcacgagtt ccgcggcgac
    14521 ccactgaggg gtgaagtcgg catcggcgac gatgatcgcg ccccccgccg ccaccgctcc
    14581 ccgcgcattg agccgctgtt cgccgttgcc ggccgcatag ggcacgaata ccgacggaac
    14641 accgagagcc gtcacctcag cgaccgtcga cgtgccggcc cgcgagagcg acaggtcggc
    14701 cacggcgagg gcgaggtcca tgcggtcgca gtacttgaga actcggtacc cgggaagacg
    14761 ggggtcgtct acggcttcgc ggaactcgcc cgtcacgtgc agaacctgcc agcccacgcc
    14821 gagaatggtc ggcgccgacg ccagcacggt ctcgttgatg cgctgtgcgc ccgacgagcc
    14881 acccgtcacc agaagagtgg gacgatcctc gacgagatcg aagagccgga tgccctcccg
    14941 cctggccgcg aaccggtcga gtcgctcgat ctccggccgc agaggcatgc ccacccacgt
    15001 ggcgtgcgga atgcgggtgc cctcgaacgc gacgcccacg aagggcgtga accgcgctcc
    15061 gagacgattg gcgatgcccg gacgggcgtt ggcctcgtgc acgacgagcg gcgtcgactc
    15121 gtcgcgcgcg gccaggtacg cgggggcggc ggcgtagccg ccgaagccga cgacgacgtc
    15181 gatgccgcgg ctggtgatga tggagcgaac ctcagcgacc gcagcgcgca aacggcccgg
    15241 aaacgtcagc gccgcacggc cgggtcggcg cggaaacgga agacgttcga ttgtgacgag
    15301 ctcgtagccg cgctccggca cgagccgcgc ctcgagaccc tcgcgagtgc ccagcacgag
    15361 aatcgtcgac gcggggtcac gctcgcgaag tcgatcggcc acggcgagca gcggattgac
    15421 atggccggcg gttccgccgc cagccagcag gtaggtggtc accggcgggc cgcccgattg
    15481 cttgcgagaa ggcgcgaacg ttgcgccgga gtctgtgcca cgccctgatc gagggcgctc
    15541 tcacgatcgg tctggcgggc gaacgagaga accacgccga ttccgagaag aatcgcgatg
    15601 agcgcggagc cacccgcgga gatcaggggc agcggaacac cgagcaccgg cagcaggccc
    15661 agcacaacgg cgatgttcac gagggcctgt ccgatcaccc agaccatgac gccccccgtg
    15721 gtgatcctcg cgaagctgtc ccgggtggcg cgcagaaccc gcacgaaggc aatcgccaga
    15781 acgatgaaca gcaccaggac gactatcgct ccgatcagac cgagctcctc gccgatgatg
    15841 gcgaagatga agtcgttgtc tgcctcggga agccagttcc acttcgcctt cgaattcccc
    15901 aagccgcttc cgaagaggcc gcccgcggcg agtgcccaac tcgcgtgctg cacctgccag
    15961 cagaggttgg agtagtcggc ggcggtgcat ccgttgagcc agacgctgat gcgctcggtg
    16021 cgcgacggcg tcgcgagagc catgatgatg accaacaggc tcacgccggc gagagcgaca
    16081 gccaggaact tcaggcgcac tccggcgaag tacaggcacc ccagcacgat catgacgatg
    16141 atggatgccg tgcccaggtc cttgccgatc agcacgaaca agatggcgac gcccgccacc
    16201 ggagcgatcg ggagcgcgac atgtcgccag tcgccgagca gctcctcctt ggtcgacagc
    16261 acccaggcaa tccacacgat gagcgcgatc ttgacgaact ccgacggttg catgttgaag
    16321 ccgccgatgg agatccagtt ctggttgccg ccgtgctcga gacccagccc cgggatgaag
    16381 acgatgagtt gcagcgcgcc gccgacgagg agtgccggcc atgcccagcg tttccagaac
    16441 cgcggcggca tccgcgaggc gacgagcatg agcggaatgc cgaggatcgc cgagaacccc
    16501 tgccggatga atcgaccgaa gaagccctca ccgtcgacgc tcgactcgat ggacgacgac
    16561 gacagcacca tcacgaggcc gaacgccacc aggaacaggg tggtgcccag gagcaggaag
    16621 tagttgcggt tctcgatcgc gaagatgcga cgaacgggga tcactgccac agcgggtcgc
    16681 ggtgcggcgg cggttggtgc ggcggggcga cccgggcgcc gtgacactgt gacccgaggg
    16741 gacgacggga gccggccggt tgtcatcgat tcacctctcc ccctggtcag gagacggcac
    16801 gctgaaccgt cccgcgcgcg ggatgtaccc ctgaagtctc ggtcgaaacg ggtcgccggt
    16861 gggtgacgtc gggcgcgtgt cgcgggaatg acgcccagtt cagccggtgg tgggcatgaa
    16921 tgtcgctagc gcagtgcgca cctcggcggg cgagacgccg tgtgctcgcg caacggcgca
    16981 cgccgcgagc acctggggca gcagctcggc ggtggcaagg tcggcctccg ccagctcgcc
    17041 atgcgtggtg agttcgagcg ccgtgctgtg acgctcatcg aggaaggcgc gatcgacgac
    17101 gatgtcgccg accatgccca gatcgctgcg cccgggagcg ccgagaccaa agccgatagc
    17161 ccggcagccc tcctgcacgt cggcgtcctc gaccaggtgc atcgtcgcct cgtcggcctg
    17221 gttgtagacg caggcgatcg ccgtgttctc gtagaccctg ccgaagacct cctgtggaag
    17281 gtcggacgcg gggtcgacgg tgaggcagac gctgacctca ggacgcaggg cgcccgggcc
    17341 cgagcgagtg atccactgca actgtgccgg cgacagcgcc acgatgaggc cgtcgaaacc
    17401 cacgggatca cgcacggcgt cgagcgcgag catccccacc gaacccgcaa ccacgtaccg
    17461 tcgcccgccg ccgacgagta gatgagcggc gagcaggctc gtcatctcga cgccgctcga
    17521 gcccgctatc aggttccagt gcgcgggcgg tgcgaccttg tccctgagcc gccaggcgag
    17581 ttcgacatca ccccagatcg ggataccgcc ctctcgcgcc caagcgacga gcgggtgatc
    17641 cggggcgtac ccgggcgaca caacgatgag ctcagggtcg aaggaggcca ggtcgggcgg
    17701 gatcacctcg cgatctggct gcgggttgta tcgcgctccg atcacggaga ggagatcaat
    17761 gcgttcctcg tcggcctcgg cggcgaccac cagcaccgca cagcgaagtt cggtgagcgt
    17821 gtcggcggcg gcgaaaccgg tggcacccag cccgagcacc gccactcgca gagaactcca
    17881 gtcggcgtac cagctggt
//
LOCUS       DAOSOU010000016        17576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3252_length_17576_cov_5.707151, whole genome shotgun sequence.
ACCESSION   DAOSOU010000016 DAOSOU010000000
VERSION     DAOSOU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 17576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17576
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_17576_cov_5.707151"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLW15_01710"
     CDS             <1..637
                     /locus_tag="PLW15_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT33147.1"
                     /translation="AAIAYAVADQLGFAKDDVVWVRTTFDEAIAPGPKNFDFNLQQYS
                     ITDERKQNVDFSSPYYETTQVVITTGASKAASAKSIADLKGLLIGAATGTTSFDAIEQ
                     LIAPTTGAQAFNSNDDAKLALESGTVDAIVVDLPTAFYLTGVELTDGIIVGQLPLADG
                     KSGDEFGLVLAKGSPLTAKVTAAVDALRDNGTLAEIAATWLADAANAPVLK"
     gene            657..1532
                     /locus_tag="PLW15_01715"
     CDS             657..1532
                     /locus_tag="PLW15_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOT33148.1"
                     /translation="MTSHDADREARQPSSVELDRRAFRRRQSLRSVLISIASTLVLAV
                     AAWLFLINTPGWAQVQRTFFDPQVFVSALPRVWDGFLLNLRVLFFAVIGVFVVAVLIA
                     VIRTLRGPVFFPLRALAAGYTDLFRGMPLIIVLYLVGYGIPGLQGADGARIPAAVLGT
                     IALILTYSAYVSEVFRAGIEAVHPSQRLAARSLGLTHAQSLRLVILPQAVRKVTPALM
                     NDFIAMQKDVGLISVLGAVDAVRAAQIETAKFFNFTPYVLAGLLFVLLALPMIRLTDW
                     YTARLREREQVGGIV"
     gene            1529..2272
                     /locus_tag="PLW15_01720"
     CDS             1529..2272
                     /locus_tag="PLW15_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOT33149.1"
                     /translation="MTTEPVLRLQNIHKSFGSTQVLRGVDVTVDAHEVVALIGASGSG
                     KSTLLRTINLLERVDDGQVWLQGVDISDPRTVVDQVRARIGVVFQHYNLFPHLTVLDN
                     VTLASRRVFKQSRDAAEAHGLELLERIGLAPQARSYPDRLSGGQQQRAAIARAVATNP
                     ELLLLDEITSALDPELVTEVLDLVRELATEGTTIVMATHEMAFARDVADRVVFLDSGV
                     VAEQGAPAEVFGAPREERTKAFLSRFNAG"
     gene            complement(2259..3071)
                     /locus_tag="PLW15_01725"
     CDS             complement(2259..3071)
                     /locus_tag="PLW15_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT33150.1"
                     /translation="MSSFTDWTADDAATRIAVPGLHDDKYSRGVLGVITGSQQYPGAA
                     VLGVEAALHTGVGMVRYLGPSVPAQLVLARRPEAVTSAGRVQAWLLGSGMDPVDRDVA
                     SMTDALAQALPTVLDGGALDLHDRVGGPVVITPHYRELARVLGADAERIAADPEHWAR
                     VAADDLGVTVLLKGHTTLVAGPGVSLRVRSAPTWLATAGAGDALGGILGALVATHSDA
                     VRDDPDELARLAATASVIHGLAAERAGGGGPFTILGLCSALPATVAELLSRR"
     gene            3096..3713
                     /locus_tag="PLW15_01730"
     CDS             3096..3713
                     /locus_tag="PLW15_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT33151.1"
                     /translation="MTLSIRDRVVVFDYGEVISLSPSDKDRSELLAISGCDAAPFWDS
                     YWRHRDGLDQGTVTIPDYWALVAADTGSAWDAPTVHALWVADFRSWWSVEPGTLRLIE
                     ELHAGGTRLAILSNAGFDFASGFRFSPMGTYFERVFVSAEMDAIKPDPAIYLEVADEL
                     GITPAEMIFIDNKSVNTDAAAALGITTHHFTSVAGLDAFLRSLAA"
     gene            3710..4798
                     /locus_tag="PLW15_01735"
     CDS             3710..4798
                     /locus_tag="PLW15_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOT33152.1"
                     /translation="MSALFTPMTLRSVTARNRLWVAPMCQYSVELEDGVPTTWHATHL
                     GSFAVGGAGLVMTEATAVTPDGRISPQDTGIWNDAQRDAWAPIAAFIRAQGAIAAIQL
                     AHAGRKASTWRPWSGEHGTVPASAGGWQPVAPSAVAFEGYATPRELDASELPDIVAAF
                     ASAARRAIDAGFELLEVHAAHGYLLHQFLSPLSNLRDDEYGGSLENRARLVLEIVRAV
                     RAEVGADVPIFVRFSATDWTEGGWNKEDTKTVARWAVEAGADLFDISSGGLVGGVTIP
                     VGKGYQVPLAAHVHEPDLPVSAVGMITEAQHAEEIVATGEADAVMVAREMLRDPHFAL
                     RAAAELGVAVDYVPKQYLRAPYPGPSAS"
     gene            complement(4810..5850)
                     /locus_tag="PLW15_01740"
     CDS             complement(4810..5850)
                     /locus_tag="PLW15_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT33153.1"
                     /translation="MMDWWGIAWLGVLLLINAFFVGAEFAVISARRSQIEPLAEAGSR
                     AAKTALWAMEHATLMLAACQLGITVCSLLILNVSEPAIHHLLEVPLALTGWSEEVIGT
                     VAFIIALVLVSFLHVVFGEMVPKNISFSLPDRAALILAPPLVFIARIFNPLIVALNAI
                     ANGILRLFRVTPKSEANSIFTLDEVATIVAQSTREGVLTDAGGTLNAAFEFTSKKVQD
                     VAVGMSALVTLPEDATPADVERAVAQRGFSRYILVDEGGEPTGYLHLKDVIDLDDADE
                     FTEPVPPKRIRQLISIFRGTDLEDALATMRRSGVHVARAFDESGSTKGVLFLEDIIEE
                     LVGEVQDATRRR"
     gene            complement(5847..7160)
                     /locus_tag="PLW15_01745"
     CDS             complement(5847..7160)
                     /locus_tag="PLW15_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT33154.1"
                     /translation="MSELIMLGVGLLLTIGTGLFVASEFALVNLDRSDLEVRQSRGER
                     GLGTVISALKQTSTHLSSAQLGITLTTLLAGYTLEPAFSSWLSVPLTALGLSVPAVRI
                     IASILAITVATLLSMIIGELVPKNLALALPRAVAKVVVPFQVGFTAVFRPAVALLNST
                     ANAILRLIGIEPKEELSGARTAEELTSLVRRSAREGSLDRDTATLLARTLAFADHTAQ
                     DVMTPRPRVASVERTDTAQDVIELARRTGFSRFPVIDDGIDDVVGIVHIKQAVAVPRE
                     KRADVPVSALQSDALRVPETMKLDSLLAELRGRGYQMAVVVDEYGGTAGVTTLEDLVE
                     ELVGEVSDEHDRTKADVVRSRDWFTFPGILRPDELLERTDVVVPEEGPYETVAGWLMS
                     ELGRLPKVGDTVEIDRGVFRIERLEGRRIDRVRYTPRREPAEGDA"
     gene            complement(7247..8683)
                     /locus_tag="PLW15_01750"
     CDS             complement(7247..8683)
                     /locus_tag="PLW15_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HOT33155.1"
                     /translation="MRFYETTPSHDLTYSDVFLVPERSAVTSRLTVSLAPEDGTGATI
                     PIVSANMNSVTGPRLAATLARRGGLGVIPQDLPATELDAAIRWVKAQPVAYDAPVDLG
                     PGDTVAAALALLPPLAGHGIIVHDEHGEFLGAIAAERLATALPDARLGDLVARVTEID
                     VDELDGPRAAFDLMVAADLQFAPVLHEGKVVGALSRKGALRSTLYDPALDAQGRLRVA
                     AAIGINGDVAGKAKAIVAAGADVLVIDTAHGHQDAMVRVINTVASLGLGVPIVAGNVV
                     TADAVRDLVGAGATIIKVGVGPGAMCTTRMMTAVGRPQFSAVLETAQAARSLGAHVWA
                     DGGVRYPRDVALALAAGASSVMIGSWFAGTIEAPGTLAIDEQGRAYKESWGMASTKAV
                     RERFDKLDAYELARKTLFAEGISSSKIYLDPLRPSLEDLLDMITSGVRSSLSYAGATT
                     LAEFHERALVGVQSAAGYEEGKALPVSW"
     gene            8815..12618
                     /locus_tag="PLW15_01755"
     CDS             8815..12618
                     /locus_tag="PLW15_01755"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HOT33156.1"
                     /translation="MSSQVTGIAPEGDSSGEFGANEWLVDEMYELYLQDKNLVDSTWW
                     PVLENYHRTVADPTPTGTIPTVTDAAAPAAESPAVAAPEAPAPAAAAPAPAAPAAAPA
                     TGNIPVARTTSLQAKPQPIPADAPATSPIVTPATDVDPAAEGENVVTVLKGATKSLAA
                     NMDASLSVPTATSVRTIPAKLMIDNRIVINNHLKRARGGKVSFTHLIAWAIVQTLKEF
                     PSQNVFYDEVDGKPSVVTPAHVNLGIAIDIPKPDGTRSLVVPGVKRADTMGFGEFLTA
                     YEDVVARGRANKLTAADYAGNTISLTNPGGIGTEHSVPRLMKGAGCIVGAGALEYPAE
                     FQGASPKTLAELAIGKTITLTSTYDHRVIQGAGSGEFLKKIHERLIGEHHFYEDIFAA
                     LRIPYDPIHWAADISVDLADNVDKTARVQELINAFRVRGHLMADIDPLEYRQRSHPDL
                     DISSHGLTFWDLDREFVTGGFGGKRTMLLREILGVLRDSYCRTIGIEYMHIQDPEQRR
                     WFQEHVEQKYTKPDHDMQMRILGKLNEAEAFETFLQTKYVGQKRFSLEGGESLVALLD
                     TVLQKSAEANLEEVAIGMAHRGRLNVLTNIAGKTYGQIFREFEGTQDPKTVQGSGDVK
                     YHLGTEGTFTAAHGETIPVYLAANPSHLEAVDGVLEGIVRAKQDRRPIGSFTVLPVMV
                     HGDAAMAGQGIVVEILQMSQLRAYRTGGTIHVNINNQVGFTTPPAEGRTSTYSTDVAK
                     TIQAPIFHVNGDDPEAVMRVGELAFAYRQQFHRDVVIDLICYRRRGHNEGDDPSMTQP
                     LMYNLIEAKRSVRTLYTEALVGRGDITQEEYEAAHRDFQDSLERAFAETHAAQTGSMP
                     IVGAASVSDLEKPGSQQDDAVGEPATTGVSESIVRVIGDAHDNIPAGFTVHPKLQALL
                     KKRVEMSRSGSIDWGFGELLALGSVLLEGTPVRMVGQDTRRGTFVQRHAVFHDRENGQ
                     EWLPLANLAENQARFWIYDSLLSEYAAMGYEYGYSVERADALVLWEAQFGDFANGAQT
                     VIDEFISSAEQKWGQRSSVVLLLPHGYEGQGPDHSSARIERYLQLCAENNMTVARPST
                     PASHFHLLRRQAYARPRRPLVVFTPKAMLRLKGATSEVSDFTSGRFEPVIDDSRVTDK
                     SAVKRVILTSGKIYYDIIAELDKREEKSVAVVRMEQYYPTPISEINAALEQYPNADIV
                     WTQDEPENQGAWPFICLELVKHLKGRTIKVASRPASASPATGSSKRSAQEQADLIEKA
                     LTLGQ"
     gene            complement(12652..13056)
                     /locus_tag="PLW15_01760"
     CDS             complement(12652..13056)
                     /locus_tag="PLW15_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOT33157.1"
                     /translation="MKTLLNVIWLVLCGFWLFLGYALAGVICCILIVTIPWGIASFRI
                     ANYVLWPFGRQVVQKPTAGAFSFLGNVIWFIFAGLWLAIGHVISGVALAITIIGIPLA
                     IADFKMIPISLAPLGKEIVSTGGTTIVAPQTT"
     gene            complement(13062..13292)
                     /locus_tag="PLW15_01765"
     CDS             complement(13062..13292)
                     /locus_tag="PLW15_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate decarboxylase"
                     /protein_id="HOT33158.1"
                     /translation="MTDCGCDKAKAELEEYLHRELSQQDFADITEHLAGCDDCSAEHL
                     VGITLTLKVQKACKETAPEELRAAIMARLENA"
     gene            complement(13289..13978)
                     /locus_tag="PLW15_01770"
     CDS             complement(13289..13978)
                     /locus_tag="PLW15_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT33159.1"
                     /translation="MATMLDARQNVILGDLELALMDIDEVEPAAALDGPEDKKRLFEE
                     QALPFMDQLYAAAMRMTRNPADAADLVQETFVKAFQAFAQFQQGTNLKAWLYRIQTNT
                     FINNYRKNQRNPYQGTIDDLEDWQLGGAESVTQSLSTRSAEAEAIDHLPDSTVKEALQ
                     AIPEDFRLAVYFADVEGFSYQEIADIMKTPVGTVMSRLHRGRRMLRDMLSDYARERGI
                     PVAPATTRSTT"
     gene            14029..15417
                     /gene="aroA"
                     /locus_tag="PLW15_01775"
     CDS             14029..15417
                     /gene="aroA"
                     /locus_tag="PLW15_01775"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT33160.1"
                     /translation="MQVFRYSGPEFSPYGDDERVATTDPGPWRAPVASAPLKATLALP
                     GSKSLTNRELVLSALADGPSLLRAPLHSRDTALMVEALRSLGVGIREVPTDSVFGPDW
                     EITPAPLEGGTSIDYGLAGTVMRFLPPVASLALGPVAFDGDASARRRPMRTTIDSLRA
                     LGVDVTDDGRGALPFSLYGTGEVAGGDLEIDASASSQFVSGLLLAAPRFTNGLRLRHT
                     GERLPSMPHIEMTIACLAARGVTVESPEPGVWVVAPGPIAGATIDIEPDLSNAAPFLA
                     AAVVAGGSVTITGWPASTTQVGADLAHLLPHFGATVTRDGDALTVTGGSEITGVDLDL
                     STGGELAPALVALAALATGPSRITGIGHLRGHETDRLAALTAEISGLGGSVTEHEDGL
                     DLEPSALHGGAWRSYEDHRMATAGAIIGLAVPGVEIDDIATTAKTLPQFPALWAAMLA
                     EPHATVETIGII"
     gene            15419..16480
                     /gene="rsgA"
                     /locus_tag="PLW15_01780"
     CDS             15419..16480
                     /gene="rsgA"
                     /locus_tag="PLW15_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT33161.1"
                     /translation="MSWLTNDDDDGDYAEYDESAAKVRPNPKGNKPRSKNRPEHADAV
                     SGRVFSVDRGRYGVWVDENTPEERQIVATKARELGKTSLDRAADGKVSVVTGDWVDLV
                     GDVTGDEGSLARIVRIQPRSTLLRRSADDTDAVERIIVANADQMLIVVAAKDPEPRPR
                     LVDRYLVAAFDAGITPLLCITKTDLADPTEFLANFAGLDITIFRSRSDDFPLEEIRAA
                     LQGHTTVAVGHSGVGKSTLVNALVPEANRATGRVNDVTGRGRHTSSSTVSYRIGSGWI
                     VDTPGVRSFGLGHVDPANILRSFTDLAAIAEDCPRGCTHLPDSPDCAIIERVNAGELG
                     EIGKLRLDSFQRLITTLGS"
     gene            16477..17268
                     /gene="hisN"
                     /locus_tag="PLW15_01785"
     CDS             16477..17268
                     /gene="hisN"
                     /locus_tag="PLW15_01785"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOT33162.1"
                     /translation="MTDYTTSDDLALALTLAADADHISMDRYRSVDLVVTTKPDRTPV
                     TDADQAVERSIRTGIEAARPHDSILGEEYGTQGSGSRQWIIDPIDGTANFLRGVPIWG
                     TLIALAVDGVPTVGVVSSPALGKRWWGATGHGAWAQETGHEPRALRVSRVAELADASL
                     SYNSMQGWDEVGRLDDVVALSRAVWRSRAIGDMWSYMLLAEGALDIVGEFDLKPYDMA
                     ALIPIVEEAGGTFTSVTGIPGPWEGNAIATNGLLHDRVLELLAAR"
     gene            17397..>17576
                     /locus_tag="PLW15_01790"
     CDS             17397..>17576
                     /locus_tag="PLW15_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33163.1"
                     /translation="MTTLPRTRLARTLAAVTIAAASFGLTGCSLLGNVVADPTPSANP
                     TDGTETDAFAIGVGDC"
BASE COUNT         2918 a         6178 c         5769 g         2711 t
ORIGIN      
        1 ggccgccatc gcctatgcgg tcgctgacca gctcggcttc gccaaggacg acgtcgtgtg
       61 ggtgcgcacc accttcgacg aggccattgc gcccggcccg aagaacttcg acttcaacct
      121 ccagcagtac tcgatcaccg acgagcgcaa gcagaacgtc gacttcagct cgccgtacta
      181 cgagacgact caggtcgtga tcacgaccgg cgcctcgaag gccgcgagcg cgaagagcat
      241 cgccgacctc aagggactcc tgatcggcgc cgcgaccggc acgacgagct tcgacgccat
      301 cgagcagctg atcgctccca ccaccggcgc ccaggccttc aactccaacg acgatgccaa
      361 gctggcgctc gagagcggca ccgtcgacgc gatcgtcgtc gacctcccca ccgcgttcta
      421 cttgaccgga gttgaactca ccgacggcat catcgtgggc cagctccccc tcgccgacgg
      481 caagagcggc gacgagttcg gtctggtcct ggccaaggga agcccgctga ccgccaaggt
      541 cacggccgcg gtcgatgccc tgcgcgacaa cggaaccctc gccgagatcg ccgcgacctg
      601 gcttgcggat gccgcgaacg cacccgttct gaagtagttc cagtagtttc gtcagggtga
      661 cctcccacga cgcagaccgc gaggcccggc agccgagttc agtagaactc gaccgccggg
      721 ccttccggcg tcggcagtcg ctgcggtccg tgctcatcag catcgccagc acactcgtgc
      781 tcgccgtcgc ggcgtggctg ttcctgatca acacccccgg ctgggcgcag gtgcagcgca
      841 ccttcttcga tccgcaggtg ttcgtgtctg ccctgcctcg cgtgtgggac ggcttcctcc
      901 tgaacctgcg agtgctgttc ttcgccgtga tcggcgtctt cgtcgtcgcg gtgctgatcg
      961 ccgtcatccg caccctgcgc ggtccggtgt tcttcccgct gcgggccctt gccgcgggct
     1021 acaccgacct gttccgcggc atgccgctca tcatcgtgct gtacctcgtc gggtacggca
     1081 tccccggact tcaaggagcc gatggagcac ggatccccgc ggccgtgctc ggcacgatcg
     1141 cgctgatcct cacctactcc gcctacgtgt ctgaggtgtt ccgcgcgggc atcgaggcgg
     1201 tgcatccgtc acagcggctc gccgcgcgat cgctcgggct cacccacgcg cagtcccttc
     1261 ggctggtcat cctcccgcag gcggtgcgca aggtcacccc ggcgctcatg aatgacttca
     1321 tcgcgatgca gaaggacgtc ggcctgatct cggtgctcgg agccgttgac gcggtgcgcg
     1381 ccgcgcagat cgagaccgcc aagttcttca acttcacgcc ctacgtgctc gctggcctgt
     1441 tgttcgtgct gctggcgctg ccgatgatca ggctcaccga ctggtacacc gcacggctcc
     1501 gtgagcgcga gcaggttggc ggcatcgtat gacgaccgaa ccggttctgc ggcttcagaa
     1561 catccacaag tcgttcggct cgacccaggt gctgcgaggc gtcgacgtga ccgtggacgc
     1621 ccacgaggtg gtcgcgctga tcggcgccag cggctccggc aagtcgactc tgctgcgcac
     1681 catcaacctg ctcgagcgtg tcgacgacgg gcaggtgtgg ctgcagggcg ttgacatcag
     1741 cgacccccgc accgtggtgg accaggtacg cgcgcgcatc ggcgtcgtgt tccagcacta
     1801 caacctgttt ccgcacctca ccgtgctcga caacgtgacc ctcgcctcac gccgggtgtt
     1861 caagcagtcc cgggatgccg ccgaggctca cggcctcgaa ctgctcgagc gcatcggact
     1921 cgcgccgcag gcccgctcct accccgaccg cctgtcgggt ggacagcagc agcgtgccgc
     1981 gatcgcccga gccgtggcga ccaaccccga gttgctgctg ctggacgaga tcacgagcgc
     2041 cctcgacccc gagctggtga cagaggtgct cgacctggtg cgcgagctgg cgaccgaggg
     2101 aacaacgatc gtcatggcca cccacgagat ggccttcgcg cgggatgtcg ccgaccgcgt
     2161 cgtcttcctc gacagcggcg tggttgccga gcagggtgct ccggctgagg tgttcggcgc
     2221 tcctcgcgag gagcgcacga aggcgttcct ctcccgcttc aacgccggct gagcagctcg
     2281 gccacggtcg ccggcagcgc cgagcagaga ccgagaatcg tgaacgggcc accaccgccg
     2341 gccctctcgg ccgcgaggcc gtggatgacc gacgcggttg ccgcgagacg ggcgagctcg
     2401 tcggggtcgt cacgcaccgc atccgagtgg gtcgcaacga gcgccccgag gatgcccccg
     2461 agcgcgtccc cggcacccgc ggtcgcaagc cacgtgggcg ccgagcgcac ccgcagcgag
     2521 acacccgggc cggcgacgag cgtggtgtga cccttgagca gaacggtcac accgaggtcg
     2581 tcggcggcca ctcgcgccca gtgctcaggg tcggcggcga tgcgttcggc gtcggcgccc
     2641 agaacccggg cgagctcccg gtagtgcggc gtgatgacca ccgggcctcc cacccggtca
     2701 tggaggtcga gggcaccccc gtccagcacc gtcggcagtg cctgagccag ggcatccgtc
     2761 atggatgcga cgtcgcgatc gaccgggtcc attcccgagc ccagcagcca ggcctgcaca
     2821 cgccccgctg aggtgacggc ctcgggacgc ctcgcgagca ccagctgggc gggaaccgag
     2881 ggtccgaggt agcgcaccat gccgaccccg gtgtgcagcg cagcctcgac gccgagcacc
     2941 gcagcgcccg ggtactgctg ggatcccgtg atcacgccga gcaccccgcg gctgtacttg
     3001 tcgtcgtgga gcccgggcac cgctatgcgg gtggccgcgt catccgcggt ccagtcggtg
     3061 aaggagctca tggctcccac gatagtttgg atggtgtgac cctgagcatt cgtgaccgcg
     3121 tcgtcgtctt cgattacggg gaggtcatct cgctgtcgcc ctccgacaag gatcgctccg
     3181 aactgctggc catctccgga tgcgacgccg cccccttctg ggactcctac tggcgccatc
     3241 gcgacggcct cgaccaggga accgtcacaa ttcccgacta ttgggccctg gttgccgccg
     3301 acaccgggtc ggcctgggac gcgcccacgg tgcacgccct ctgggtggcc gacttccgca
     3361 gctggtggag cgtcgaaccg ggcacccttc ggctgatcga ggagctccac gccggcggaa
     3421 cccggctggc gatcctctcc aacgccggct tcgacttcgc cagcgggttc cggttctccc
     3481 cgatgggaac ctacttcgag cgcgtcttcg tgagcgccga gatggatgcg atcaagcccg
     3541 atccggccat ctatctggag gttgccgacg agctgggaat aaccccggcc gagatgatct
     3601 tcattgacaa caagtccgtc aacaccgacg cggccgccgc gctcgggatc accacgcatc
     3661 acttcacgtc tgtggccggc ctcgacgcct tcctgaggag cctcgccgca tgagtgcctt
     3721 gttcaccccg atgacactgc gatcggtcac cgcccgcaac cggctctggg tcgccccgat
     3781 gtgccagtac tcggtcgagc tggaggacgg cgtgccgacc acctggcacg cgacgcatct
     3841 cggatcgttc gcggtgggtg gcgcgggcct ggtcatgacc gaggcgaccg ccgtgacccc
     3901 ggacgggcgc atctccccgc aggacacggg aatctggaac gatgcccagc gtgacgcgtg
     3961 ggcgccgatc gccgcgttca tccgcgccca gggcgcgatc gccgccatcc agttggcgca
     4021 cgcgggccgc aaggcatcga cctggcgccc gtggtccgga gagcacggca cagttccggc
     4081 gagcgccggc ggctggcagc cggttgcgcc gtcagccgtg gccttcgagg gctacgccac
     4141 gccccgcgag ctggatgcct ccgagctccc cgacatcgtc gccgccttcg ccagcgcggc
     4201 gcgacgggcc atcgacgccg gattcgagct cctcgaagtg catgcggcgc acgggtacct
     4261 gctgcaccag ttcctctcgc cgctgtcgaa cctccgcgac gacgagtacg gcggcagcct
     4321 cgagaaccgc gcccgactcg tgctcgagat cgtgcgagcc gttcgcgccg aagtcggcgc
     4381 ggatgtgccg atcttcgtgc gcttctccgc caccgactgg accgagggcg gctggaacaa
     4441 ggaggacacc aagaccgtcg cgcgctgggc ggtcgaggcg ggtgccgacc tgttcgacat
     4501 ctccagcggc gggctcgtgg gcggcgtgac catccccgtg ggcaagggct accaggtgcc
     4561 gctcgctgcg cacgtgcacg aacccgacct gccggtcagc gcggtcggca tgatcaccga
     4621 ggcgcagcac gccgaggaga tcgtcgccac gggtgaggcc gacgcggtca tggtcgcgcg
     4681 cgagatgctc cgcgacccgc acttcgccct gcgggccgcc gccgaactcg gcgtcgccgt
     4741 cgactacgtg cccaagcagt acctgcgggc gccgtatccc gggccgagcg ccagttaggc
     4801 ggcgccggtc tagcgacggc gggtcgcgtc ctgcacctcg ccgaccagtt cctcgatgat
     4861 gtcctcgagg aacagcacgc ccttcgtgga gccgctctcg tcgaaggcgc gggcgacgtg
     4921 cactcctgag cgccgcatcg tggcgagggc gtcttcgaga tcggttccgc ggaagatcga
     4981 gatgagctgc cggatccgct tcggcggcac cggctcggtg aactcgtcgg catcatcgag
     5041 atcgatgacg tccttgaggt gcaggtagcc ggtgggctcg cccccctcgt ccacgaggat
     5101 gtagcgcgag aacccgcgtt gcgccacggc acgctccaca tccgcgggcg ttgcgtcctc
     5161 gggcagggtg acgagcgccg acatgccgac cgcgacatcc tgaaccttct tggaggtgaa
     5221 ctcgaaggcg gcgttcaggg tgccgccggc gtcggtgagc accccctccc tggtcgactg
     5281 ggcgacaatc gtggccacct catcgagggt gaagatgctg ttcgcctccg acttcggggt
     5341 aacccgaaag agccgaagaa tgccattggc gatcgcgttg agcgcgacga tcagcgggtt
     5401 gaagatgcgg gcgatgaaca cgaggggcgg agcgaggatg agggccgcac ggtcgggcag
     5461 cgagaagctg atgttcttgg gcaccatctc cccgaacacc acgtgcagga acgaaaccag
     5521 caccagcgcg atgatgaagg cgacggttcc gatcacctcc tcagaccacc ccgtgagggc
     5581 cagcggcacc tcgagcagat gatggatcgc gggctccgac acgttgagga tcagcagcga
     5641 gcacacggtg atgcccaatt ggcaggccgc gagcatgagc gtggcgtgct ccatcgccca
     5701 cagtgccgtc ttcgccgcac gtgaccccgc ctcggccagc ggttcgatct gcgagcgccg
     5761 cgccgagatc acggcgaact cggcgccgac gaagaaggcg ttgatcaaca ggagcacgcc
     5821 cagccaggcg attccccacc agtccatcag gcgtcaccct ccgcaggttc gcggcgcggt
     5881 gtgtaccgca cgcggtcgat gcgtcgaccc tcgagccgtt cgatgcggaa cacaccgcga
     5941 tcgatctcca cggtgtcgcc gaccttcggt agccgaccga gctcgctcat cagccagccg
     6001 gcgacggtct cgtagggacc ctcctcgggt accacgacgt cggtgcgttc gagcagttca
     6061 tcggggcgca ggatgcccgg aaaggtgaac cagtcgcgtg agcgcacgac atcggccttg
     6121 gtgcggtcgt gctcgtcgct gacctcaccg acgagttcct cgacgagatc ctcgagggtg
     6181 gtgaccccgg cggtgcctcc gtactcgtca acgacgaccg ccatctggta accccggccg
     6241 cggagctcgg cgagcagcga atcgagcttc atcgtctcgg gcacgcgcag ggcatccgac
     6301 tgcagggctg agacgggaac gtcggcgcgc ttctcccgag gcacggcgac cgcctgcttg
     6361 atgtgcacga tgccgacgac atcatcgatc ccgtcatcga tgacgggaaa gcgcgagaag
     6421 cccgtgcgac gggccagctc gatgacatcc tgcgcggtgt cggtgcgctc gacgctggcg
     6481 acgcgggggc ggggcgtcat cacatcctgt gcggtgtggt ccgcgaaggc gagtgtgcgg
     6541 gcgagcagcg tcgcggtgtc gcgatcgagg ctgccctcgc gcgccgaccg ccgcacgagc
     6601 gaagtgagct cctcagccgt gcgcgcaccc gacagctcct ccttcggctc gatgccgatg
     6661 agtcggagga tcgcgttggc ggtcgaattg aggagggcca ctgcggggcg gaagacggcg
     6721 gtgaatccca cctggaatgg cacgacgacc ttcgccaccg cccgtggcag agcaagggca
     6781 agattcttgg gcaccagctc gccgatgatc atcgacagca gcgtcgcaac cgtgattgcg
     6841 aggatcgacg cgatgattcg cacggccggc accgataggc cgagggcggt cagcggcacc
     6901 gacagccacg agctgaaggc gggttcgagc gtgtaccccg ccagcagggt ggtgagggtg
     6961 atgccgagct gcgcgctgga gagatgggtg gacgtttgct tgagcgcgga gatgactgtg
     7021 ccgagtccgc gctcgcctcg cgactgccgt acctcgagat cggaccggtc gagattgacc
     7081 agggcgaact ccgaggcgac gaacaacccc gtgccgatgg tcagcagcaa gcccacgccg
     7141 agcatgatga gctcagacat gcgcggcacc ggcccggcga ctagagggag ggtcgtccat
     7201 tatctggtca aggataccgg atcacaggtc caggagcccg tcgagatcac cagctgacgg
     7261 gaagcgcctt gccctcctcg tatccggccg ctgactgcac acccacgagc gcccgttcgt
     7321 ggaactcggc gagcgtcgtg gcacccgcgt agctcagcga gctgcgcacg cccgaggtga
     7381 tcatgtcgag gagatcctcg agcgacgggc gcagcgggtc gagatagatc ttcgagctcg
     7441 agatgccctc ggcgaagagg gtcttgcgag cgagctcgta cgcatccagc ttgtcgaagc
     7501 gctcccgcac ggccttggtg gaggccatgc cccagctctc cttgtaggcc cggccctgct
     7561 cgtcgattgc gagagttccc ggggcttcga tggttcccgc gaaccacgac ccgatcatga
     7621 cggatgacgc gcccgccgca agcgccagtg cgacgtcgcg cgggtagcgc accccgccgt
     7681 cggcccacac gtgggcaccg agggaccgcg cggcctgggc cgtctcgagc accgctgaga
     7741 actgcgggcg accgaccgcc gtcatcatgc gggtcgtgca catcgcaccg ggacccacgc
     7801 ccaccttgat gatcgttgcg ccagcgccga ccagatcgcg caccgcatcg gcggtgacca
     7861 cgttgcccgc cacgatcggc acgccgagtc cgagggatgc gaccgtgttg atcacccgca
     7921 ccatggcgtc ctgatgaccg tgcgccgtgt cgatcacgag cacgtcggca ccggcagcga
     7981 cgatcgcctt cgccttgccc gcgacgtcac cgttgatgcc gatggcggca gcgacacgaa
     8041 gtcgcccctg ggcgtcgagt gccgggtcgt acagggttga gcgcagggca cccttgcggc
     8101 tgagcgcacc aaccaccttg ccctcgtgca gcactggggc gaactggagg tctgccgcca
     8161 ccatgaggtc gaaagccgcc cgcggcccgt cgagctcgtc gacatcgatt tcggtcacac
     8221 gggcgacgag gtcgcccagc ctggcgtcgg gcagggcggt ggccagccgt tcggcggcaa
     8281 tcgcgccgag gaactcgccg tgctcgtcgt gcacgatgat gccgtgcccc gccagcggcg
     8341 gcagcagggc gagagccgcg gcgacggtgt caccggggcc gagatcgaca ggtgcgtcgt
     8401 aggccacggg ctgggccttg acccagcgga tcgcggcatc cagctcggtc gcaggaaggt
     8461 cctgaggaat gacgccgagc ccgccacggc gtgccagcgt cgccgccagc cgtggcccgg
     8521 tcaccgagtt catgttcgcc gagacgatcg gaatggtcgc gccggtgccg tcctcgggcg
     8581 ccaaggagac cgtcaggcga ctcgtgacag ccgaccgctc cggcacgagg aacacgtcgg
     8641 agtaggtgag atcgtgggag ggcgtggtct cgtaaaagcg catgaattta acgctagtgc
     8701 gcccggcgac gaacagcggg cacgcagcaa aagccattac tctagaggcg gtgtgtctta
     8761 tgtggtgcac gcccttaagt tgtgacgaga agaactgaaa gtgggcggtc ggctgtgtca
     8821 agccaagtga ccggaatcgc acccgagggt gactcctcag gcgaattcgg agccaacgaa
     8881 tggctcgttg atgaaatgta cgagctctac ctccaggaca agaacctcgt cgacagcacg
     8941 tggtggccgg tgttggagaa ctaccaccgc acagtcgcag accccacccc caccggcacg
     9001 attccgaccg tgacggatgc cgcggccccc gccgccgagt cccccgccgt tgccgcaccc
     9061 gaggcccccg caccggccgc tgccgcaccc gcacccgccg cacccgccgc cgcgccggcg
     9121 accggaaaca tcccggtcgc gcgcacgacc tcacttcagg cgaagccgca gccgattccc
     9181 gccgacgccc cggcgacgtc gcccatcgtc accccggcaa ccgacgtcga ccccgcagcc
     9241 gagggtgaga acgtcgtcac cgtgctcaag ggcgcgacca agtcgttggc cgccaacatg
     9301 gacgcgagcc tgtcggttcc cacggcaacc agcgtgcgca ccatccccgc caagctgatg
     9361 atcgacaacc gcatcgtcat caacaaccac ctcaagcgcg cccgcggtgg caaggtctcc
     9421 ttcacccatc tcatcgcgtg ggcgatcgtg cagacactca aggaattccc cagccagaac
     9481 gtcttctacg acgaggtgga tggcaagccc tcggtggtca ccccggcgca cgtcaacctc
     9541 ggtatcgcga tcgacatccc caagcccgat ggcacccgtt cgctcgtggt gcccggcgtc
     9601 aagcgcgccg acaccatggg cttcggcgag ttcctcaccg cctacgagga tgtcgtcgcc
     9661 cgcggtcgtg ccaacaagct caccgccgcc gactacgccg gcaacaccat ctcgctgacc
     9721 aacccgggcg gcatcggcac cgagcactcc gttccgcgcc tcatgaaggg cgccggctgc
     9781 atcgtcggag ccggtgcgct cgagtacccg gccgagttcc agggcgccag ccccaagacc
     9841 ctggcggagt tggcgatcgg caagacgatc acgctcacga gcacctacga ccaccgcgtc
     9901 atccagggtg cgggctcggg cgagttcctc aagaagatcc acgagcggct catcggcgag
     9961 caccacttct acgaggacat cttcgcggcg ctgcgcatcc cctacgaccc gatccactgg
    10021 gccgcggaca tcagcgtcga cctcgccgac aacgtcgaca agaccgcgcg cgtgcaggaa
    10081 ctcatcaacg ccttccgggt gcgaggccac ctcatggccg acatcgaccc gctggagtac
    10141 cgccagcggt cgcatcccga cctcgacatc tcgagccacg gcctcacctt ctgggacctc
    10201 gatcgcgagt tcgttaccgg tggtttcggc ggcaagcgca cgatgctgct gcgcgagatc
    10261 ctgggtgtgc tgcgtgactc ctactgccgc acgatcggca tcgagtacat gcacatccag
    10321 gacccggagc agcgccgctg gttccaggag cacgtcgagc agaagtacac caagcccgac
    10381 cacgacatgc agatgcgcat cctcggcaag ctcaacgagg cggaggcctt cgagacgttc
    10441 ctgcagacca agtacgtcgg ccagaagcgg ttcagcctcg agggcggcga gtcgctcgtg
    10501 gcccttctcg acaccgtgct gcagaagtcg gccgaggcca acctcgaaga ggtcgccatc
    10561 ggcatggccc accgcggccg cctcaacgtg ctcaccaaca tcgcgggtaa gacctacggt
    10621 cagatcttcc gcgagttcga gggcacccag gatcccaaga ccgtgcaggg ctcgggagac
    10681 gtgaagtacc acctcggcac cgagggcacg ttcactgccg cccacggcga gaccattccc
    10741 gtctacctcg ctgccaaccc ctcgcacctc gaggccgtcg acggcgtgct cgagggcatc
    10801 gtgcgtgcca agcaggaccg ccgtccgatc ggctcattca ccgtgctgcc cgtgatggtg
    10861 cacggtgacg cggccatggc cggtcagggc atcgtcgtgg agatcctcca gatgtcgcag
    10921 ctgcgggcgt accgcaccgg cggcaccatc cacgtcaaca tcaacaacca ggtcggattc
    10981 accacgccgc cggccgaggg tcgcacctcg acctactcga ccgacgtcgc caagacgatc
    11041 caggcgccga tcttccacgt gaacggtgat gaccccgagg ccgtcatgcg cgtcggtgag
    11101 ctcgccttcg cctaccgcca gcagttccac cgcgatgtcg tcatcgacct gatctgctac
    11161 cgccgccgcg gacacaacga gggcgacgac ccgtcgatga cccagccgct catgtacaac
    11221 ctgatcgagg cgaagcgttc ggtgcgcacc ctctacaccg aggccctcgt cggtcgcggt
    11281 gacatcaccc aggaggagta cgaggccgcg caccgcgact tccaggacag cctcgagcgc
    11341 gcattcgccg agacccatgc cgctcagacc ggctcgatgc cgatcgtcgg cgcggcatcc
    11401 gtgtccgacc tggagaagcc cggctcccag caggatgacg cggtgggcga gcccgccacc
    11461 actggcgtga gcgagtcgat cgtgcgggtc atcggtgacg cccacgacaa catccccgcc
    11521 ggattcactg tgcaccccaa gctgcaggct ctcctgaaga agcgcgtcga gatgagccgc
    11581 agcggctcga tcgactgggg cttcggcgag ctgctcgccc tcggctcggt gcttctcgag
    11641 ggaacgccgg tgcgcatggt cggccaggac acccgccgtg gcacgttcgt tcagcgccac
    11701 gcggtgttcc acgaccggga gaacggtcag gagtggctgc ccctggccaa cctcgccgag
    11761 aaccaggctc gcttctggat ctacgactcg ctgctcagcg agtacgccgc catgggctac
    11821 gagtacggct actcggtgga acgcgccgac gcgctcgtgc tctgggaggc ccagttcggt
    11881 gacttcgcca acggcgccca gaccgtgatc gacgagttca tctccagcgc cgagcagaag
    11941 tggggccagc gctccagcgt cgtcctgctg ctgccgcacg gctacgaggg ccagggaccc
    12001 gaccactcgt cggcgcgcat cgagcgctac ctgcagctgt gcgcggagaa caacatgacc
    12061 gtagcgcggc cgtcgactcc ggcgtcgcac ttccacctgc tgcgccgcca ggcgtatgca
    12121 cgcccccgtc gcccgcttgt cgtcttcacc cccaaggcga tgctgcgcct caagggtgcg
    12181 accagtgagg tctccgactt caccagtggt cggttcgagc cggtgatcga tgacagccgg
    12241 gtcaccgaca agtcagccgt gaagcgcgtc atcctgacct cgggcaagat ctactacgac
    12301 atcatcgccg agctcgacaa gcgcgaggag aagtcggtcg cggtcgtgcg gatggagcag
    12361 tactacccca caccgatctc ggagatcaac gccgccctcg agcagtaccc gaacgccgac
    12421 atcgtctgga cgcaggacga gccggagaac cagggtgcct ggccgttcat ctgcctggag
    12481 ctcgtcaagc acctcaaggg ccgcacgatc aaggtcgcct cgcgaccggc atccgcgtcg
    12541 cccgcgaccg gctcgtcaaa gcgcagcgcc caggagcagg cggacctgat cgagaaggcg
    12601 ctgacgctag ggcagtgacc ctctggggcg ccgaggttca cctgacgttg gtcaggtggt
    12661 ctgcggggcc acgatcgtcg tgccgccggt cgacacgatc tccttgccca gcggggcgag
    12721 cgagatggga atcatcttga agtccgcgat cgccagcggg atgccgatga tcgtgatcgc
    12781 gagggcgaca ccggagatga cgtggccgat cgccagccac agccccgcga agatgaacca
    12841 gatcacgttg ccgagaaacg agaaggctcc ggccgtgggt ttctgcacca cctgccgacc
    12901 aaacggccac agcacgtagt tggcgatgcg gaacgaggcg atcccccacg ggatggtcac
    12961 gatcaggatg cagcagatga cgccggcgag ggcatagccg aggaacagcc agaatccgca
    13021 gagcacgagc cagatgacgt tgaggagcgt cttcacgtca ctcaggcatt ctcaaggcgg
    13081 gccatgatgg ccgcgcgaag ctcctcgggc gccgtctcct tgcacgcctt ctgcaccttg
    13141 agggtcaacg tgatgccgac gaggtgctcg gcgctgcagt cgtcgcagcc cgcgaggtgc
    13201 tcggtgatgt cggcgaagtc ctgctggctc agctcgcggt gaaggtactc ttcgagctca
    13261 gccttggcct tgtcgcaacc gcagtcggtc atgtcgtgct cctggtggtg gccggggcga
    13321 ccgggatgcc ccgctcgcgt gcatagtcgg agagcatgtc acgcagcatt cgcctgccac
    13381 ggtgcagacg gctcatgacg gttccgaccg gggtcttcat gatgtcggcg atctcctgat
    13441 aggagaagcc ctccacgtcg gcgaaataga ccgccagccg gaagtcctcg gggatcgcct
    13501 gcagggcttc cttgaccgtg ctgtccggca ggtggtcgat cgcctcagcc tcggccgatc
    13561 gggtcgacag cgactgggtg accgactcgg cgccgccgag ctgccagtcc tcgagatcgt
    13621 cgatggtgcc ctgatagggg ttccgctggt tcttgcggta gttgttgatg aaggtgttgg
    13681 tctggatgcg gtacagccag gccttgaggt tcgtgccctg ttggaactgc gcgaacgcct
    13741 ggaacgcctt cacgaacgtc tcctgcacga ggtcagcggc gtcggcggga ttgcgggtca
    13801 tccgcattgc ggcggcgtag agctggtcca tgaagggaag cgcctgctcc tcgaagagac
    13861 gcttcttgtc ttcgggcccg tcgagtgcgg cggccggttc gacctcatcg atatccatca
    13921 gcgccaattc taggtcgccg aggatcacgt tctgccgtgc atccagcatc gtggccatct
    13981 cgtgcggtct cctcccggca tgctcaagtg ccgatactgt ggtaaccgat gcaggtattc
    14041 aggtattccg ggcccgagtt cagcccctac ggggacgacg agcgggtcgc cacaaccgac
    14101 ccggggccgt ggcgtgcgcc cgtggcatcc gcgccactga aggcgacgct cgcgctgccc
    14161 ggatccaaga gtctgaccaa tcgcgagctc gtgctctccg cactggccga cggaccgtcg
    14221 ctgctgcgcg cacccctgca ctcgcgcgac accgccctga tggttgaggc gttgcgctcg
    14281 ctcggtgtgg gcatccgcga ggtgccgacc gacagcgtct tcgggcccga ctgggagatc
    14341 acgccggcgc cgctcgaggg cggcacctcc atcgactatg ggctcgccgg cacggtcatg
    14401 cggttcctgc cgcccgtcgc ctcgctcgcg ctcggccctg tcgccttcga cggggatgcc
    14461 tcggcacgcc gtcgcccgat gcgcacgacc atcgactccc tgcgcgccct cggggtcgac
    14521 gtcaccgacg acggccgggg tgcactgccg ttcagcctct acggcacggg cgaggtggcc
    14581 ggtggggacc tggagatcga cgcgtctgcg tcgagccagt tcgtctccgg actgctgctg
    14641 gcggccccgc gcttcaccaa cggactgcgt ctgcggcaca ccggcgaacg cctgccgagc
    14701 atgccgcaca tcgagatgac catcgcctgt ctggcggcgc ggggcgtgac ggtcgaaagc
    14761 ccggagcccg gggtgtgggt ggtcgccccc ggaccgatcg ctggtgcgac catcgacatc
    14821 gagcccgatt tgtcgaatgc cgcgcccttc ctggccgcgg ccgtcgtcgc aggcggatcc
    14881 gtgaccatca ctggctggcc cgcgagcacg acccaggtgg gtgcggatct tgcgcacctg
    14941 ctcccccact tcggcgccac ggtgacccgc gacggcgatg cgctgaccgt gacgggcggc
    15001 tcggagatca ccggcgtcga cctcgacctg agcaccgggg gtgaactggc gcccgccctg
    15061 gtcgcgctcg cggcgctggc caccgggccg agccggatca ccggcatcgg tcacctgcgc
    15121 gggcacgaga ccgatcgtct ggccgcgctc acagcggaga tcagcgggct cggcggatcg
    15181 gtcaccgagc acgaggacgg cctcgacctc gagcccagcg cgcttcacgg cggcgcctgg
    15241 cgcagctacg aggaccaccg catggcgacc gccggcgcga tcatcggcct ggccgtgccg
    15301 ggcgtggaga tcgacgacat cgccacgact gcgaagactc ttccccagtt ccccgccctc
    15361 tgggccgcga tgctcgcgga gccccacgcg acagtcgaga cgatcggcat catctagcgt
    15421 gagttggctg acgaacgacg acgacgacgg cgactacgcc gagtacgacg agtcggcggc
    15481 caaggtgcgc ccgaatccca agggcaacaa gccccgcagc aagaaccgcc cggagcacgc
    15541 cgacgcggtg agcggtcggg tgttcagtgt ggaccgcggc cggtacggcg tctgggtcga
    15601 tgagaacacc cccgaggagc gccagatcgt cgccacgaag gcccgcgagc tcggcaagac
    15661 ctcgctcgat cgcgcggccg atggcaaggt ctcggtcgtg accggagact gggtcgacct
    15721 cgtgggcgat gtcaccggtg acgagggcag tctggctcgt atcgtgcgca tccagccgcg
    15781 ctccaccctg ctgcgccgca gcgccgacga cacggatgcc gtggagcgca tcatcgtcgc
    15841 caacgccgac cagatgctca ttgtcgtcgc cgcgaaggac cctgagccgc gcccgcgact
    15901 cgtcgaccgc tatctggtcg ccgccttcga cgccgggatc acgccgctgt tgtgcatcac
    15961 caagaccgac ctcgccgacc ccaccgagtt cctcgccaac ttcgcggggc tcgacatcac
    16021 gatcttccgc agccgctccg acgacttccc gctcgaggag atccgcgccg ccctgcaggg
    16081 gcacaccacc gttgccgtcg gccactccgg ggtcggcaag tcgaccctcg tgaatgcgct
    16141 cgtgcccgag gcgaaccggg ccaccgggcg cgtcaacgac gtgacgggcc gggggcggca
    16201 cacgtcgtca tccaccgtgt cgtaccgcat cggcagcggc tggatcgtcg atacgcccgg
    16261 tgttcggtcg ttcggcctcg gacatgtcga ccccgccaac atcctcaggt cgttcaccga
    16321 tctcgccgcg atagccgagg actgcccgcg cgggtgcacg catctgcccg actcccccga
    16381 ctgcgccatc atcgagcgcg tgaatgccgg agaactcggc gagatcggca agctgcggct
    16441 ggattccttc cagcgcctca tcactacgct gggttcgtga ccgactacac cacctccgac
    16501 gacctcgccc tcgccctcac actcgccgcc gacgccgacc acatctcgat ggatcgctac
    16561 cgttcggtcg acctggtcgt gacgaccaag cctgaccgca cgcccgtgac ggatgccgac
    16621 caggcagtcg agcgcagcat ccgcaccggc atcgaggccg cgcgaccgca cgactcgatc
    16681 ctcggcgagg agtacggaac ccagggttcc ggatcacggc agtggatcat cgacccgatc
    16741 gacggcaccg ccaacttcct gcgcggtgtg ccgatctggg gcacgctgat cgcgctggcc
    16801 gtggacggcg tgccgacggt cggcgtcgtc tcgtcgccgg cgctcggcaa gcggtggtgg
    16861 ggcgcaaccg ggcacggcgc gtgggcgcag gagaccggcc acgagccgcg cgccctccgc
    16921 gtcagccggg tggctgagct cgccgacgcc tcgctcagct acaacagcat gcagggctgg
    16981 gatgaggtgg gtcgcctcga cgacgtcgtc gcgctgtcgc gtgcggtctg gcgatcccgc
    17041 gccatcggcg acatgtggtc gtacatgctg ctcgcggagg gagcgctgga catcgtgggc
    17101 gagttcgacc tgaagcccta cgacatggcc gcgctgattc ccatcgtcga ggaggcgggt
    17161 ggcacattca cctcggtgac gggcatcccg ggcccttggg agggcaacgc gatcgccacc
    17221 aacggcctgc tgcacgacag ggtactcgag cttctcgcag cgcgctgacc ggggcgcacc
    17281 gaacgccgac ggagccgggc gttctggtcg cgcgttctgg ccgagcattc ggccattcgc
    17341 tacgctggag cacgtccttt gagcatccgc agcacgagaa aacgaggcga ccgtccatga
    17401 ccactcttcc ccgcaccaga ctggctcgca cccttgcggc agtcacgatc gccgccgcga
    17461 gtttcggcct cacgggctgc agcctgctcg gtaacgtcgt tgccgacccc acccccagcg
    17521 ccaaccccac cgacggcacc gagaccgacg cctttgcgat cggcgtcggc gactgc
//
LOCUS       DAOSOU010000017        17273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3321_length_17273_cov_5.692066, whole genome shotgun sequence.
ACCESSION   DAOSOU010000017 DAOSOU010000000
VERSION     DAOSOU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 17273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17273
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3321_length_17273_cov_5.692066"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLW15_01795"
     CDS             <1..812
                     /locus_tag="PLW15_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33164.1"
                     /translation="TGLDDLDKVVHVDQAPIGRTPRSNPATYTGVFDKIRTLFSETAD
                     AKARGYLPGRFSFNVKGGRCENCAGDGTIKIEMNFLPDVYVACEVCGGARYNRDTLAV
                     KYKGKNIAEVLDMPISEAAEFFEAISAIHRFLKTLEEVGLGYVRLGQSATTLSGGEAQ
                     RVKLATELQRRSMGRSIYVLDEPTTGLHFEDVRKLLAVLNGLVDKGNTVIVIEHNLDV
                     IKSADWLIDLGPEGGAGGGEVLSIGTPEQLAKVKGSHTGIFLKETLEGARA"
     gene            826..2691
                     /gene="uvrC"
                     /locus_tag="PLW15_01800"
     CDS             826..2691
                     /gene="uvrC"
                     /locus_tag="PLW15_01800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT33165.1"
                     /translation="MAQTVSWRPKAGEIPTQPGVYRFRDATSRVLYVGKAKNLRARLS
                     NYFAPLSTLHDRTRKMVQTATSVEWTVVGNDFEALQLEYTWIKEFDPPFNVQYKDDKS
                     YPYLAITLGDPVPRVLVTRNRSIPNARYFGPYSRAWAIRETVDIMLKAFPMRSCSDAT
                     YKRAQQTGRPCLLGDIGKCAAPCVARVTLEEHKSIALDFASFMGGNDSRYVTTITRRM
                     REAAQAQEYELAARYRDQIAAMETALAKNAVVLDDAVDADLFGIAHDELAAAVQQFIV
                     RGGRIRGVRGWVVDKELDVELPDLVESVLQNAYEDATPPRDILVPALPDDAPALEQWL
                     GALRDPSSGTRVELKVARRGDKAALAQTVAQNAMNALVLYKSRRSADFVARSQALSDI
                     QEALGMDEAPLRMECFDVSHLSGTNIVASMVVFEDGLPRKDQYRRFSIPESSDDTESI
                     HQVLTRRLAYLRQPEVDDDEGAPRRKKFSYPPQLLIVDGGQPQVAAAQRALDEAGVTG
                     IRLAGIAKRLEEIWLPDSDYPVILPRNSDALFLIQRIRDEAHRFAISYQRQKRRNDIQ
                     TVLADVPGLGPSRVKELLKHFGSVSRLKTADAAAVAEVRGIGPVLAQVVVERLRS"
     gene            2757..3629
                     /gene="rapZ"
                     /locus_tag="PLW15_01805"
     CDS             2757..3629
                     /gene="rapZ"
                     /locus_tag="PLW15_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT33166.1"
                     /translation="MIEPTTQEMLIVTGMSGAGRSTVGNALEDLGWYVVDNLPPQMLR
                     PLVELAQHAGNSLPRIAAIVDVRGGKLFSDVQQAIELLRDNAMVRVLFLDATDATLVR
                     RFEQVRRPHPLQGDGTLLDGIAEERSRMAEIRESSDVVIDTSELNIHQLATTITEKFS
                     EENAAGLRVTVMSFGFKYGVPTDADLVADARFLPNPFWDPELKALTGRDQKVSDYVLS
                     QPGAAEFVASYAAAMAPVFAGYQRENKRHATIAIGCTGGKHRSVAVAEELATLLRSLP
                     GVAVSVKHRDLGRE"
     gene            3687..4664
                     /gene="whiA"
                     /locus_tag="PLW15_01810"
     CDS             3687..4664
                     /gene="whiA"
                     /locus_tag="PLW15_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOT33167.1"
                     /translation="MALTAEVKEELGAIQSGKTTVRAAELATILRFSGGLHVISGRIA
                     VESEVESEQLAKRIRKDIAELYGVRAEITIVSASGVRRTSHYLVRVLDGGETLARQTG
                     LLDARRRPIRGLPNRLTTGSREDLAAVWRGAFLASGSLTDPGRSAVLEVTCPGNEAAM
                     ALVGAAGRLRIAAKAREVRGIHRVVVRDGEAIGAMLELMGATKGVAAWNELRQRREVR
                     ATANRLVNFDDANLRRSAQAAVAACARVERALEILDGDVPEHLRYAGELRLRFRDSSL
                     DELGHHADPPMTKDAVAGRIRRLLAMADKRASDLGLPGTDANLPPDLDE"
     gene            4779..5405
                     /locus_tag="PLW15_01815"
     CDS             4779..5405
                     /locus_tag="PLW15_01815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOT33168.1"
                     /translation="MADYTLPELAYDYSALAPSISATIMELHHSKHHQAYVTGANAAL
                     AAMADARESGELANINKLEKDLAFNLGGHVNHSIFWTNMSPDGGDKPTGDLASAIDDN
                     FGSFDKFTAHFTAAAMGVQGSGWAALTWDSIGQKLLINQFFDQQSNFAAGTVPLLLLD
                     VWEHAYYLDYKNVRADYVKAFWNIANWANVQERFTVAREKTDGLLVLS"
     gene            5500..6507
                     /gene="gap"
                     /locus_tag="PLW15_01820"
     CDS             5500..6507
                     /gene="gap"
                     /locus_tag="PLW15_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT33169.1"
                     /translation="MSVKIGINGFGRIGRNFFRAALAKGSDLEIVAVNDLTDNKTLAH
                     LLKYDSITGRLDATVEFDGEQIVVNGKAIRVFEERDPANLPWGELGVDIVIESTGRFT
                     KSEDAAKHITAGAKKVIVSAPASGADVATLVLGVNEGTYDPAVHNIISNASCTTNCLA
                     PLAKVFMEAFGIERGLMTTVHAYTADQNLQDGPHSDLRRARAAAHNIIPTSTGAAKAL
                     GLVIPELVGKLDGYALRVPVITGSITDLTVTASRPVTVEEVNAAYKAAAEGPLKGILK
                     YTEDEIVSSDIVGDPHSSIFDAGLTKVIGDQVKVASWYDNEWGYSNRLVDITEYVGAR
                     L"
     gene            6514..7731
                     /locus_tag="PLW15_01825"
     CDS             6514..7731
                     /locus_tag="PLW15_01825"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT33170.1"
                     /translation="MSLRTIDSLGDLRGKRVIVRCDLNVPLKNGQITDDGRIRASLPT
                     LNALLHAGARVIVVSHLGRPDGEPDSKYSLEPVAQRLSELLGKPVVFAHDTVGSGAEE
                     AVEGLADGDIALLENLRFNAHETTKDDSDREHFAKKLARFGDAFVSDGFGVVHRKQAS
                     VYELAQQLPSAAGLLIQEELSVLERLTVTPEKPYTVVLGGSKVSDKLAVIGALLPKVD
                     TLLIGGGMMYTFLVAQGHKVAASLLELDQVDAVKGYLEQAQKLGVEIVLPTDVVVAAS
                     FSGDADHVVAPADAIEDTPFGASGLGLDIGPDTSARFAEVISASRTVFWNGPMGVFEL
                     PAFAAGTKAVASALASFDGFGVVGGGDSAAAVRALGFHDDQFGHISTGGGASLEFLEG
                     KRLPGLEVLGWQQ"
     gene            7719..8516
                     /gene="tpiA"
                     /locus_tag="PLW15_01830"
     CDS             7719..8516
                     /gene="tpiA"
                     /locus_tag="PLW15_01830"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT33171.1"
                     /translation="MAAVKRVPLIAGNWKMNLDHLQAVAFVQKLAWTLKDANHDFGAG
                     GSEVAVFPPFTDLRSVQSLVSGDKLDVVFGAQDVSEFDSGAYTGEISGAFLKALECSY
                     VIIGHSERRTMHGETDEQLGRKVAAAIKHGLVPVLCVGETADDLEKHGPSAVPVAQLK
                     AALADIKGTPDIVIAYEPVWAIGSGLAATPEQAEQVAAKLRETLAELYGADVADATRI
                     LYGGSVKSGNIASLMREPNVDGALVGGASLDVAEFASIARFQKHVGT"
     gene            8569..8817
                     /gene="secG"
                     /locus_tag="PLW15_01835"
     CDS             8569..8817
                     /gene="secG"
                     /locus_tag="PLW15_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT33172.1"
                     /translation="MQILQVVLQVILGITSLLLTLLILLHRGRGGGLSDMFGGGVTSN
                     LGASGVAERNLNRITVILALVWITCIVVLGLITKFDGA"
     gene            8826..9185
                     /locus_tag="PLW15_01840"
     CDS             8826..9185
                     /locus_tag="PLW15_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOT33173.1"
                     /translation="MASGGSAIRGSRVGAGPMGEQDRGYHADRIQVSYWDEMGNETVR
                     NFAANLPDEEIPDIIDSPSTGLPAGRDKNNPPSVAKLEPYKTHLAYVKERRTEEEAAT
                     LLEEALTQLRERRGTAS"
     gene            complement(9182..9979)
                     /gene="pgl"
                     /locus_tag="PLW15_01845"
     CDS             complement(9182..9979)
                     /gene="pgl"
                     /locus_tag="PLW15_01845"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOT33174.1"
                     /translation="MSNERRVILHPDKDALAAAVAARFLTKIVDLIDEFDEASVALTG
                     GTMGIAVLAAINASPARDSVDWSRLNVWWGDERWLPAGDPDRNETQARAALLDHVALD
                     PARVHPMGASDAGLSLDDAADAYAADLALNARRGHVPYLDITFLGMGPDGHIASLFPE
                     RSGIRESERTVIAVRNSPKPPPERLSLTLPVLNSSARVWLVVAGADKASALGLTLAGA
                     SINEVPAAGIEGRRRTLFFVDAEAAAEVPENLIDPGQFWTRADDIAD"
     gene            complement(9976..10944)
                     /locus_tag="PLW15_01850"
     CDS             complement(9976..10944)
                     /locus_tag="PLW15_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HOT33175.1"
                     /translation="MIVDLPNTTTSAVSKTLVKIREEGGAVALGRVLTLIISTALGDE
                     EEAIEAANDASREHPMRVLVVSTSPTPTDDEARVDAQIRVGGDAGASEVVVMRAYGDA
                     ATDPESLIMGLLLPDAPVVAWWPGEAPAAVSESAIGRIAQRRITDAASSSDPLGALAR
                     IATTYQPGDTDFSWTRLTLWRAQLAAVLDQPPYLPVTAVEVHGASDSPSTVLLAAWLQ
                     LQLKVKVAREVSTKAHGSSGIQGVRLDRSAGPIMLERTVANTATLVQPDQPTHDISLQ
                     RRSLRDCLAEELRRLDPDDLFGDVVMKGIGQLNAPTTGTHTIVGAS"
     gene            complement(10941..12482)
                     /gene="zwf"
                     /locus_tag="PLW15_01855"
     CDS             complement(10941..12482)
                     /gene="zwf"
                     /locus_tag="PLW15_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT33176.1"
                     /translation="MSPVEISADFNPLRLPSDRRLNRIAGPSGLVIFGVTGDLSRKKL
                     MPAVYDLANRGLLPPGFALVGFARRDWEDEDFAEVVHDAVKKYARTPFDEDVWAQLAQ
                     GIRFVQGEFDDASAFEDLKQTIEDLDRDRGTMGNHAFYLSIPPKSFPQVTEQLRNSGL
                     AEQQDGQWRRVVIEKPFGSDLVTARELNAVVESVFPPDSVFRIDHYLGKETVQNILAL
                     RFANQLYEPIWNANYVDHVQITMAEDVGVGGRAGYYDGIGAARDVIQNHLLQLLALTA
                     MEEPVSFDAADLRAEKEKVLSAVRLPKDLGKHTARGQYSGGWSGGEKVVGFLEEEGMN
                     PQSTTETFAAIRLDIGTRRWAGVPFYLRTGKRLGRRVTEIAVVFKRAPQYLFAESQTS
                     ALGQNALVIRVQPDEGVTIRFGSKVPGAGMQVRDVTMDFGYGHAFTEASPEAYERLIL
                     DVLLGDPPLFPRHEEVELSWKILDPIEEYWAKQGQPEQYRPGTWGPPSADALMARDGR
                     SWRRP"
     gene            complement(12503..14101)
                     /locus_tag="PLW15_01860"
     CDS             complement(12503..14101)
                     /locus_tag="PLW15_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT33177.1"
                     /translation="MSVKIAVSGAAAEAVDRVVPQLVSDLVASRITGGDETLWGPDAE
                     EESAKRLGWVEAVSVSRPMVADIVALRDKLREDGVDHIVLGGMGGSSLAPEVITRTYG
                     VELTVLDSTDPGQVLAALGDRLATTAVVISSKSGSTVETDSQKRIYEKFFSEAGIDPL
                     ERIIVVTDPGSPLDAAARATGYRVFNADPNVGGRYSALTAFGLVPSGLAGVDLTELLD
                     EAEAVMVELAVDNTANPGLVLGAAIAGTLPLKDKLGIVPDGTHILGFGDWAEQLIAES
                     TGKLGKGILPVVLDVNSPELSENLPDLQIVRLVGDWDDTREVADGEIEIAGSLGGQIL
                     VWEYATAVAGRLLGINPFDQPDVESAKIATRALIDHPSEPAAPAFVDGDIEVRGTADV
                     VSGATDLTGAISSLLSHLTDDGYVSIQAYVDRVAHPALHALRDALAARAKRPVTFGWG
                     PRFLHSTGQFHKGGPAVGVFLQITTVEDTDLQIPERPFTFGQLIRAQADGDASVLAEH
                     GRPVLSLTLRNPGASIPVVVSALN"
     gene            complement(14098..15210)
                     /gene="tal"
                     /locus_tag="PLW15_01865"
     CDS             complement(14098..15210)
                     /gene="tal"
                     /locus_tag="PLW15_01865"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOT33178.1"
                     /translation="MSTESPTAALSALGVSIWLDDLSRERIASGGLQKLISERNVVGV
                     TTNPTIFASALAKGEAYDAAVGALAKAGAAVTDAVFEITTDDVAAACDIFRPIYDATA
                     GFDGRVSIEVEPGLAHDAAGTIAQAKQLWEKVDRENALIKIPATIEGLEAIAATIGAG
                     ISVNVTLIFSLERHREVINAYLTGLETAKAAGIDISTIHSVASFFVSRVDTEIDKRLD
                     AIGTDEAKALKSKAGIANARLAYEVYEQAFASERAKVLLAAGANKQRPLWASTGVKDP
                     SLPDTLYVTELAVADTVNTMPEKTLEATFDHGVIEGDQVTGSYAEANATLDALAALGI
                     SYDEVTALLEKEGVEKFIVSWNELLDTVSAALEAAK"
     gene            complement(15234..>17273)
                     /gene="tkt"
                     /locus_tag="PLW15_01870"
     CDS             complement(15234..>17273)
                     /gene="tkt"
                     /locus_tag="PLW15_01870"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT33179.1"
                     /translation="ILAADAVEKVGNGHPGTAMSLAPAAYLLFQKVMRRDPSDNAWIG
                     RDRFILSVGHSSLTQYIQLYFGGYGLELDDIKALRTWGSKTPGHPEYGHTAGVEITTG
                     PLGQGISSAVGFAYASRYERGLFDPDAPAGTSPFDHFIYVIAGDGDLQEGVSAEASSL
                     AGHQQLGNLIAIYDSNQISIEDDTNIAFTEDVKARYEAYHWHVQVVDWKKTGVYTEDV
                     AELNAAIEAAKGVIDKPSLIILRTIIGWPAPHKQNSGKIHGSALGADELAATKRVLGF
                     DPEQTFEVADEVIEHTRKAVERGKAAHAEWQVGFDAWAAANPERKALFDRLETGALPD
                     GVEAALPVFEAGKDVSTRAASGKVLGALGAVIPELWGGSADLAESNLTTIEGAASFIP
                     SVHSTHEWTGNDYGRVLHFGIREHAMAAIINGIVLHGPTRAFGGTFLIFSDYMRPAVR
                     LAALMAVPSIFVWTHDSVALGEDGPTHQPIEQLATLRAIPGFDVVRPADANEVAWAWK
                     TILERRKGPAGIALSRQNLPVFERGPGAASGDTLAAASNVSRGAYVLAEAPNGSPDVI
                     LIATGSEVQIAVEARGVLAAEGINARVVSAPSLEWFEEQDAAYRESVLPASVTARVSI
                     EAGINLTWRPYVGDRGRSVSIEHFGASADYKTLFREFGITTEAAVAAAKESLAAQ"
BASE COUNT         2954 a         5726 c         5725 g         2868 t
ORIGIN      
        1 ttaccggact cgacgacctc gacaaggtcg ttcacgtcga ccaggcgccc atcgggcgca
       61 ccccccggtc gaatcccgcg acctacacgg gcgtgttcga caagatccgc acgctgttct
      121 cggagacggc cgacgcgaag gcccgcggct atcttccggg ccgattcagc ttcaacgtca
      181 agggcggtcg ctgcgagaac tgcgccggcg acggcaccat caagatcgag atgaacttcc
      241 tgcccgacgt ctacgtcgcc tgcgaggtct gcgggggagc gcgctacaac cgcgacaccc
      301 tcgccgtgaa gtacaagggc aagaacatcg ccgaggtgct ggacatgccg atcagcgagg
      361 ccgcggagtt cttcgaggcc atctcggcca tccatcgatt cctgaagaca ctggaggagg
      421 tcgggctcgg ctatgtgcgg ctcggccaga gcgcgacgac cctgtcgggc ggcgaggctc
      481 agcgcgtcaa gctcgccacc gagctgcagc gacggtcgat gggccgcagc atctacgtgc
      541 tcgatgagcc gacgaccggc ctccacttcg aggacgtgcg caagctcctc gccgtgctca
      601 acggcctcgt cgacaagggc aacacagtga tcgtgatcga acacaacctc gacgtgatca
      661 agtcagccga ctggctcatc gacctcgggc ccgaaggtgg ggctggcgga ggagaggtgc
      721 tctcgatcgg caccccggag cagctggcca aggtcaaggg cagccacacc ggcatcttcc
      781 tcaaggagac gctggagggc gctcgcgcct agcgagcggc aacgcatggc gcagacggtc
      841 agctggcgcc cgaaggcggg tgagatcccg acccagccgg gcgtctaccg gttccgggat
      901 gccacgtccc gagtgctgta cgtgggcaag gccaagaacc tccgcgcccg cctcagcaac
      961 tacttcgccc ccctgagcac cctgcacgac cgcacccgca agatggtgca gaccgccacc
     1021 tcggtggagt ggactgtcgt gggcaacgac ttcgaggcgc tgcagctcga gtacacctgg
     1081 atcaaggagt tcgacccgcc cttcaacgtg cagtacaagg acgacaagag ctacccctac
     1141 ctggccatca cgctgggtga tcccgtgccg cgggtgctcg tcacccgcaa ccgcagcatc
     1201 ccgaacgcgc ggtacttcgg cccgtactcg cgagcgtggg cgattcgcga gacggtcgac
     1261 atcatgctca aggccttccc gatgcgcagc tgctcggacg ccacctacaa gcgcgcccag
     1321 cagacgggcc gtccgtgcct gctcggtgac atcggcaagt gcgcggcccc gtgcgtggcc
     1381 cgcgtcacgc tcgaggagca caagtccatc gccctcgact tcgcgagctt catgggcggc
     1441 aacgactcgc gctacgtgac gacgatcact cgcaggatgc gcgaagccgc ccaggctcag
     1501 gagtacgagt tggccgctcg ctaccgcgat cagatcgcgg cgatggagac cgccctcgcc
     1561 aagaacgcgg ttgtgctgga tgacgccgtc gacgccgatc tcttcggcat cgcgcacgac
     1621 gagctcgcag ccgcggtgca gcagttcatc gtgcgcggtg gacgcatccg aggcgtgcgc
     1681 ggctgggtgg tcgacaagga actggatgtc gaactgcccg atctcgtcga gtcggttctg
     1741 cagaacgcgt acgaggatgc gactcctcct cgggacatct tggtgcccgc gctgcccgat
     1801 gatgcgcccg cactcgaaca gtggctcggc gccctccgag acccgagttc cggaacacgg
     1861 gtcgagctca aggtcgcccg ccggggcgac aaggctgccc tggcccagac cgtcgcgcag
     1921 aacgccatga acgcgctggt gctctacaag tcgcgcaggt cggcggactt cgtcgcgcgc
     1981 tcacaggcgc tctcggacat tcaggaggcc ctcggcatgg acgaggcgcc gctgcgcatg
     2041 gagtgcttcg acgtctccca cctgagcggc accaatatcg tggcgtcgat ggtcgtgttc
     2101 gaagacggcc tgccgcgcaa ggatcagtac cggcggttca gcatccccga gtcatccgac
     2161 gacaccgaat cgatccacca ggtgctgacc cgccgtctcg cctacctcag gcagcccgag
     2221 gtcgacgacg acgagggcgc gcccaggagg aagaagttct cctacccacc ccagctgctg
     2281 atcgtcgacg gaggacagcc gcaggtcgcg gccgcgcagc gtgctctgga cgaggccggg
     2341 gtcacgggca tccggttggc gggtattgcg aagcgactcg aggagatctg gctgcccgat
     2401 tcggactacc ccgtgatcct tccgcgcaac agtgatgctc tcttcctgat ccagcgcatt
     2461 cgtgatgagg ctcaccgatt cgccatcagc taccaacgcc agaagcgccg caacgacatt
     2521 cagaccgttc tcgccgatgt tcccggcctg ggaccctcac gcgtgaagga gttgctcaag
     2581 cacttcggct ccgtgtcgag gctgaagacc gcagacgctg cggccgtcgc cgaggtgcgt
     2641 ggcatcggtc ccgtgctcgc ccaggtcgtc gtcgagcgtc tgcgttcctg agagcatgcg
     2701 cgtgacggcc aatcctgaca gcagctcctg agcgctgctc cggtagtctg gaaccgatga
     2761 tcgagccaac cactcaggag atgctgattg tcaccggcat gtccggtgcc ggacgctcca
     2821 cggtcggcaa tgcgctcgag gatctcggtt ggtacgtcgt cgacaacctg ccgccccaga
     2881 tgctgcggcc gctcgtcgaa ctcgcccagc acgcgggcaa ttcgcttccg cgcatcgcgg
     2941 cgatcgtgga tgtgcgcggc ggcaagctct tctccgacgt tcagcaggcc atcgagttgc
     3001 tgcgtgacaa cgccatggtg cgagtgctgt tcctcgacgc gacggatgcg acgctggtgc
     3061 gccgattcga acaggtgcgt cgtccgcacc cgctgcaggg tgatggaacc ctcctcgatg
     3121 gcatcgccga ggagcgctcc cggatggcgg agatccggga gtcgagcgac gtcgtcatcg
     3181 atacctccga actcaacatc catcagctcg cgaccacgat caccgagaag ttctctgagg
     3241 agaacgcggc agggctccgc gtcaccgtga tgagcttcgg cttcaagtac ggcgttccca
     3301 ccgacgccga cctcgtcgca gatgcgcgtt tcctccccaa cccgttctgg gatccggaac
     3361 tcaaagcgct caccgggcgc gaccagaagg tcagcgatta cgtgctcagc cagccgggcg
     3421 ccgccgagtt cgtcgcgagc tacgccgcag ccatggcccc ggtcttcgcc ggctaccagc
     3481 gggagaacaa gcgacacgct acgatcgcca ttggatgcac ggggggaaag caccgctcgg
     3541 tggccgtcgc cgaagagctc gccaccctgt tgcgcagtct tcccggcgtg gccgtcagcg
     3601 tcaagcaccg ggatctcggc cgcgagtaac gcggctacgc tgatctcgcg catcacacct
     3661 gacagacttt cagaaggagg ttcctcgtgg cacttaccgc cgaggtgaaa gaggaactgg
     3721 gagcaatcca gtccggcaag accacagtgc gagcggctga acttgccacg atcctccgct
     3781 tctccggcgg cctccacgtc atctccgggc gcatcgccgt cgagtccgag gtcgagagcg
     3841 agcaactcgc gaagcgcatc cgcaaggaca tcgccgagct gtacggagtt cgcgccgaga
     3901 tcacgatcgt ctcggcttcc ggggtgcgtc gcacgagcca ctacctcgtg cgggtactcg
     3961 acgggggtga gaccctcgcg cgccagaccg gactgctcga tgcccgccgc cgccccatcc
     4021 gcggcctgcc caaccggctc acgaccggca gccgcgagga tctcgccgca gtgtggcgtg
     4081 gcgctttcct ggcatcgggc agcctcaccg accccggccg ctctgcggtg ctcgaggtga
     4141 cctgccccgg gaatgaggcc gccatggccc tggtcggcgc tgccggccgc ctcagaatcg
     4201 ccgcgaaggc acgggaggtg cgcggcattc accgcgtcgt ggtgcgcgat ggtgaggcca
     4261 tcggggcgat gctcgagctc atgggcgcga cgaagggggt tgccgcctgg aacgagctgc
     4321 gccagcgtcg cgaggttcgt gccactgcca accggctcgt gaacttcgac gatgccaacc
     4381 ttcgccgatc cgcgcaggcg gccgtggcag cgtgtgcgcg cgtggagcgt gccctggaga
     4441 ttctcgacgg tgacgtgccg gagcacctgc ggtacgccgg cgaactgcgc cttcgcttcc
     4501 gcgactcgag cctcgacgag ctcggccacc atgccgatcc gccgatgacg aaggatgccg
     4561 tcgcggggcg gatccgtcgc ttgctggcga tggcagacaa gcgcgcatcc gacctgggtc
     4621 ttcccgggac cgatgccaat ctgccgcccg atctggacga atagtcgcga cgggaagcat
     4681 cccggagcgc cgtgcgttgg ggctactaga ctggaaaacg tcatcccgcc tcggtgctca
     4741 gatcgaggtt tcagggccta ttgctaggag attctcgaat ggctgactac acactcccgg
     4801 agctcgcata tgactactcg gcactcgcgc cgagcatcag cgccacgatc atggagttgc
     4861 atcacagcaa gcaccaccag gcatatgtga cgggcgccaa cgctgccctc gcggccatgg
     4921 cggatgcgcg cgagagcggt gagctcgcca atatcaacaa gctcgagaag gatctcgcgt
     4981 tcaacctcgg tggccacgtg aaccactcga tcttctggac caacatgtcg cccgacggtg
     5041 gcgacaagcc gaccggcgac ctcgccagcg ccatcgacga caacttcggt tcgttcgaca
     5101 agttcacggc ccacttcacc gcagcggcga tgggcgtgca gggctcgggg tgggccgcac
     5161 tcacgtggga ctcgatcggc cagaagcttc tcatcaacca gttcttcgac cagcagagca
     5221 acttcgccgc gggcacagtc ccgctcctgc tcctggatgt ctgggagcac gcctactacc
     5281 tcgactacaa gaacgtgcgc gccgactacg tgaaggcgtt ctggaacatc gccaactggg
     5341 ccaacgtgca ggagcgcttc acggtggcgc gcgaaaagac tgacggcctg ctggtactgt
     5401 catagcggca tggtggccgg ggccccgccc cggccacttc tccaccacca ccacagcatg
     5461 agatgagcgt cccgtgacgc ccgagaaact ggagtcgaat tgagcgtcaa gattgggatc
     5521 aacgggttcg gccgcatcgg ccgcaacttc ttccgcgcag cactggcgaa ggggagtgac
     5581 ctcgagatcg tcgcggtcaa cgacctcacc gataacaaga ccctcgctca cctgctcaag
     5641 tacgactcca tcaccggccg cctcgacgcc accgtcgaat tcgacggcga gcagatcgtc
     5701 gtcaacggca aggccatccg cgtgttcgag gagcgcgacc ccgcgaacct cccctggggc
     5761 gagctcggtg tcgacatcgt gatcgagtcc accggtcgct tcaccaagtc ggaggatgcc
     5821 gccaagcaca tcaccgccgg tgccaagaag gtcatcgtct ctgctccggc atccggcgcg
     5881 gatgtcgcga ctctggttct cggcgtcaac gagggcacct acgacccggc cgtgcacaac
     5941 atcatctcca acgcttcgtg cacgacgaac tgcctcgcgc cgctcgccaa ggtcttcatg
     6001 gaggcgttcg gaatcgagcg cggcctcatg accacggttc acgcgtacac ggccgaccag
     6061 aaccttcagg atggcccgca cagcgacctg cgtcgcgccc gtgcagccgc gcacaacatc
     6121 atccccacgt ccacgggtgc agcgaaggcc ctcgggctcg tcatccccga gctcgtcggc
     6181 aagctcgatg gctacgctct gcgcgttccg gtcatcaccg ggtccatcac cgacctcacc
     6241 gtcacggcga gccgtccggt cacggtcgag gaggtcaacg ccgcgtacaa ggctgctgcc
     6301 gagggccccc tcaagggcat cctcaagtac accgaggacg agatcgtctc gagcgacatc
     6361 gtcggtgacc cgcactcctc gatcttcgac gctggcctca ccaaggtcat cggtgaccag
     6421 gtcaaggtcg cgtcgtggta cgacaacgag tggggctact ccaaccgcct cgtcgacatc
     6481 accgagtacg tcggcgcacg actgtagccg gccatgtccc ttcgaacgat cgattcgctg
     6541 ggggatcttc ggggtaagcg ggtcatcgtc cgctgcgatc tgaacgtgcc gttgaagaac
     6601 ggccagatca ccgatgacgg tcgcattcgt gcgtcactgc ccaccctgaa cgcgctcctg
     6661 cacgccggag ctcgcgtcat cgtcgtctcc cacctagggc gccccgacgg tgagcccgac
     6721 tccaagtaca gccttgagcc ggtcgcccag cggctgagcg aacttctcgg caagccggtg
     6781 gtgttcgcgc acgacaccgt cgggtctggc gcagaggagg ccgtggaggg cctcgccgac
     6841 ggcgacatcg cgctgctcga gaacctgcgg ttcaacgctc acgagaccac gaaagacgac
     6901 agcgatcgcg agcacttcgc gaagaagctc gctcgcttcg gcgatgcctt cgtgtccgat
     6961 ggcttcggcg tcgttcaccg gaagcaggcc agcgtctacg agctcgcgca gcaacttccg
     7021 agcgcggccg gcctgctcat ccaggaggag ttgtcggtac tcgagcgact gacggtcacg
     7081 ccggagaagc cgtacacggt cgttctcggc ggctcgaagg tgtcagacaa gcttgcggtc
     7141 atcggggcgc tgctgcccaa ggtcgacacc ctgctcatcg gtggcggcat gatgtacacc
     7201 ttcctcgtgg cgcagggaca caaggtcgcg gcaagcctcc tcgaactcga ccaggtcgat
     7261 gcggtcaagg gatacctcga gcaggcccag aagctgggtg tcgagatcgt cctgccgacg
     7321 gatgtcgtgg tcgcggcatc gttctcgggt gacgccgacc acgtggtcgc gcccgctgac
     7381 gcgatcgagg acaccccgtt cggtgcctcg ggcctcggac tcgacatcgg tcccgacacc
     7441 tcggcgcgct tcgccgaggt gatctcggcg tcacgcacgg tgttttggaa cgggcccatg
     7501 ggcgtgttcg agctgcctgc gttcgccgcg ggaacgaagg ccgttgcatc cgctctcgcc
     7561 tcgttcgacg gcttcggcgt cgtcggcggt ggcgactccg cggccgcggt acgcgccctc
     7621 gggttccatg acgaccagtt cggtcacatt tctaccggcg gcggcgcgag cctcgagttc
     7681 ctcgagggca agcgcctgcc aggactggag gtcctcggat ggcagcagta aagcgggtgc
     7741 cgctgatcgc gggcaactgg aagatgaacc tcgaccacct gcaggcggtc gcgttcgtgc
     7801 agaagctcgc ctggacgctc aaggatgcga atcacgactt cggcgccggc ggctccgagg
     7861 tcgccgtgtt cccgccgttc accgacctgc gctccgtgca gtcgctggtg tcgggcgaca
     7921 agctcgacgt cgtcttcggc gcccaggatg tgtccgagtt cgactcgggc gcctacacgg
     7981 gtgagatctc cggcgcgttc ctcaaggcgc tcgagtgcag ctacgtgatc atcggccact
     8041 cggagcgccg cacgatgcac ggcgagaccg atgagcagct cgggcgcaag gtcgctgcgg
     8101 cgatcaagca cggtctcgtg ccggtgctgt gcgtgggcga gactgccgac gacctggaga
     8161 agcacggtcc cagcgccgtg ccggtggccc agctgaaggc ggcactcgcc gacatcaagg
     8221 gaacacccga catcgtgatc gcctacgagc cggtctgggc gatcggttca ggcctcgcgg
     8281 caacgccgga gcaggccgag caggttgcgg ccaagctccg cgagaccctc gccgagctct
     8341 acggcgccga cgtcgcggat gccacgcgca tcctgtacgg cggatcggtc aagtcgggca
     8401 acatcgcgtc actcatgcgc gagccgaacg tcgacggcgc gctggtcggc ggagcgagcc
     8461 tggatgtcgc ggagtttgcg agcatcgccc gcttccagaa gcacgtcgga acctgaaccc
     8521 gatcagcgat cgcttatact tggcaaggct tttcgaaagg gttaccccgt gcaaattctc
     8581 caggtagtgc tgcaggtcat tctcggtatc acgagcctgc tgctcaccct cctgattctg
     8641 ctccaccgcg gtcgcggcgg cggcctctcc gacatgttcg gaggcggcgt cacgtcgaac
     8701 ctcggtgcgt cgggtgtcgc tgagcgcaac ttgaaccgaa tcacggtcat tctcgctctg
     8761 gtgtggatca catgcatcgt ggttctcggg ctcatcacca agttcgacgg cgcgtaaggg
     8821 gcatcatggc atccggtggc agtgcaattc ggggttcccg cgtaggggcc ggccccatgg
     8881 gggagcagga ccgtggctac cacgcggacc gcatccaggt ctcctactgg gatgagatgg
     8941 gcaacgagac ggtccgcaat tttgctgcga acctgcccga cgaggagatc cccgacatca
     9001 tcgactcacc gtcgacgggt ctgcccgctg gccgtgacaa gaacaatccg ccgtcggtgg
     9061 ccaagctgga gccgtacaag acccacttgg cctatgtgaa ggaacgccgc accgaagaag
     9121 aggcggcaac gcttctcgaa gaggcgctga ctcagttgcg cgagcgcagg ggcaccgcca
     9181 gctagtccgc gatgtcgtcc gcgcgagtcc agaactggcc cggatcgatg aggttctcag
     9241 gaacctccgc agccgcctcg gcgtcgacga agaacaacgt ccggcgccgt ccctcgatgc
     9301 cggcggcggg cacctcgttg atgctcgcgc ccgccagcgt caacccgagt gccgatgcct
     9361 tgtcagcccc ggccaccacg agccacacgc gggccgacga gttgagcacc ggcagggtca
     9421 ggctcaaccg ctccggaggg ggcttagggg agttgcgcac ggcgatcacg gtgcgctccg
     9481 actcacggat gcccgaacgc tcggggaaca acgacgcgat gtgaccgtct ggccccatgc
     9541 cgaggaacgt gatgtccaga tacggaacat ggcctctgcg cgcgttgagc gcaaggtcag
     9601 cggcatacgc gtcggcagcg tcatccagcg acagaccggc gtcggatgcg cccatcgggt
     9661 gcacccgtgc cggatcgagg gccacgtgat cgagcaacgc cgcgcgcgcc tgggtctcgt
     9721 tgcggtcagg gtcgccagcg ggcagccaac gctcgtcacc ccaccagacg ttcagtcggc
     9781 tccagtccac cgagtcccgc gcgggggatg cgttgatcgc ggcaagaacc gcaattccca
     9841 tcgtgcctcc ggtgagcgcc accgaggcct catcgaactc gtcgatcaga tcgacgatct
     9901 tggtcagaaa ccgggcagcg acagcggcag caagtgcgtc cttgtcgggg tgcaggatga
     9961 cgcggcgctc gttgctcacg atgcccccac gatcgtgtga gttcccgtgg tcggcgcatt
    10021 caactggccg atgcccttca tgacgacgtc gccgaagagg tcatcgggat caagtcgacg
    10081 cagctcctcg gcgagacagt cccgcaggct gcggcgctgg agcgagatgt cgtgggtggg
    10141 ttggtcgggc tgcaccaggg tcgccgtgtt ggcgaccgtg cgctcgagca tgattggtcc
    10201 tgctgacctg tccagacgca ccccctggat tccgctacta ccgtgggcct tcgtcgacac
    10261 ctcgcgcgcg accttgacct tgagttgaag ctgaagccag gcggcgagaa gaacggttga
    10321 gggggagtcc gacgctccgt gcacctccac cgccgtgacc ggaaggtacg ggggctggtc
    10381 gagcacggca gcgagctgcg cccgccacag ggtcagtcgg gtccaggaga agtcagtgtc
    10441 gcccggctgg taggtcgtgg cgatgcgcgc gagcgcgccg agcggatcgg agcttgaggc
    10501 agcatcagtg atcctgcgct gcgcgattcg gccgattgcc gactccgaga ccgcagccgg
    10561 ggcctcgcca ggccaccagg cgaccaccgg cgcatccggc agcaggaggc ccatgatgag
    10621 gctttcaggg tcggtcgcgg catccccgta ggctcgcatg acgacgactt cgctcgcacc
    10681 ggcatcgcct cccacccgga tctgcgcatc cacccgcgcc tcgtcgtcgg tcggagtggg
    10741 cgaggtcgac accacgagca ctcgcatggg gtgctcccgc gaggcgtcgt tggcggcctc
    10801 gatggcctct tcctcgtcgc ccaaagcggt cgagatgatg agggtcagca cccgacccag
    10861 cgccacggca ccgccctctt cgcgaatctt gaccagcgtc ttggacacgg cgctcgtggt
    10921 cgtgttgggc agatcgacaa tcatggacgc ctccaactgc gaccgtcacg ggccatgaga
    10981 gcgtcagccg atggcggacc ccatgttccc ggtcggtact gctccggctg gccctgcttg
    11041 gcccagtact cctcgatcgg gtcgaggatc ttccaactca gttccacttc ttcgtgccgc
    11101 gggaacagcg gcgggtcacc cagaagtacg tccagaatga ggcgttcgta tgcctcgggg
    11161 ctggcctcgg tgaaggcgtg accgtacccg aagtccatgg tcacgtcgcg cacctgcatg
    11221 cccgcgcccg gaaccttcga gccgaacctg atggtgacgc cctcgtcggg ttgcacccgg
    11281 atgacgaggg cgttctgtcc gagagcagag gtctggctct cggcgaacag gtactgggga
    11341 gcgcgcttga agacgaccgc gatctcggtc acgcggcgac cgagacgctt gccggtgcgc
    11401 aggtagaagg gcaccccggc ccagcgacgg gtcccgatgt cgaggcggat ggcagcgaag
    11461 gtctcggtgg tcgactgagg gttcatgccc tcctcttcga ggaatccgac gaccttctcc
    11521 ccgcccgacc agccgcccga gtactgaccc cgggcggtgt gctttcccag gtccttcggt
    11581 agacgaaccg ccgagaggac cttctccttc tcggcgcgca ggtcggcggc atcgaatgac
    11641 accggctctt ccatggcggt cagtgccagg agctgcagca ggtggttctg aatcacatcg
    11701 cgggctgcac cgatgccgtc gtagtacccg gcgcgaccgc cgacgcccac gtcctctgcc
    11761 atcgtgatct gcacgtggtc gacgtagttg gcgttccaga tgggttcgta cagctggttg
    11821 gcgaagcgca gcgccaggat gttctgcacc gtctccttgc ccagatagtg atcgatacgg
    11881 aagacgctgt cgggcggaaa gaccgactcg accaccgcat tgagttcacg ggcagtcacg
    11941 agatcgctgc cgaacggctt ctcgatgacg acgcgtcgcc actgaccgtc ctgctgctcg
    12001 gctagcccgg agttgcgcag ctgctctgtg acctgcggga acgacttcgg cgggatcgac
    12061 aggtagaacg cgtggttgcc catcgtgccg cgatcgcgat caaggtcctc gatggtctgc
    12121 ttgagatcct cgaaggccga tgcgtcatcg aactcgccct gcacgaagcg gatgccctgc
    12181 gccagctgcg cccagacatc ctcgtcgaac ggcgtgcgcg cgtacttctt gacggcatcg
    12241 tgcacgactt cggcgaagtc ctcgtcctcc cagtcacggc gggcgaaacc gaccagcgcg
    12301 aagcccggag gcaggagtcc ccggttggcg aggtcgtaga ccgccggcat gagcttcttg
    12361 cgcgataggt cgccggtgac accgaagatc accagcccac tcgggccggc gatccggttc
    12421 agtcgacgat ccgacggcaa ccggagcggg ttgaagtcgg ccgaaatctc caccggtgac
    12481 atggtgctcc tcgcgagacg tgctagttga gtgcgctgac gacgaccggg atgctcgcac
    12541 cggggttgcg cagggtgaga ctcagtaccg gccgaccgtg ctcggcgagc acactcgcgt
    12601 cgccatcggc ctgggcgcgg atgagctgac cgaaggtgaa cggccgctcg ggaatctgca
    12661 ggtcggtgtc ctcgacggtc gtgatctgaa ggaacactcc caccgccggc ccgcccttgt
    12721 ggaactgacc ggtcgagtgc aggaagcgcg gaccccagcc gaaggtgacg gggcgcttcg
    12781 cgcgggccgc gagagcgtcg cgaagcgcgt gaagcgcggg gtgtgcgaca cggtcgacat
    12841 aggcctgaat cgacacgtag ccgtcgtcgg tgaggtggga gagcagcgac gagatggcgc
    12901 ccgtgaggtc agtcgcaccc gagacgacgt ctgcggtgcc gcgcacctcg atgtcgccat
    12961 ccacgaaggc cggtgctgcg ggctccgacg ggtgatcgat gagtgcacgg gtggcgatct
    13021 tcgcggattc cacgtcgggc tggtcgaagg gattgatccc gagcagacga cccgcgaccg
    13081 cagtggcata ctcccacacc aggatctggc caccgagact gcccgcgatc tcgatctcgc
    13141 cgtcggcgac ctcacgcgtg tcatcccagt cacccaccag gcgcacgatc tgcaggtcgg
    13201 gcaggttctc gctcagctcg ggggagttca cgtcgaggac gacgggaagg atgcccttgc
    13261 cgagcttgcc cgtcgactcg gcgatgagct gttcagccca gtccccgaag ccgaggatgt
    13321 gggttccgtc gggaacgata ccgagcttgt ccttgagggg gagggttccc gcgattgcgg
    13381 cacccagcac caggccgggg ttggcggtgt tgtcgacggc gagctcgacc atcacggcct
    13441 ccgcctcgtc gagcagctca gtgaggtcca caccggcaag tccgctcgga acgaggccga
    13501 aggcggtcag ggcggagtaa cgtccgccga cgttggggtc ggcgttgaac acgcggtatc
    13561 cggtggcgcg cgccgcggca tccaggggag aaccgggatc ggtaaccacg atgatgcgct
    13621 cgagtggatc gatgcccgcc tcggagaaga acttctcgta gatgcgcttc tggctgtcgg
    13681 tctccactgt cgagcccgac ttcgacgaga tgacgacagc ggtggtggcg agccggtcgc
    13741 ccagagcggc cagaacctgc ccgggatcgg tggagtcgag caccgtcagc tcaacaccgt
    13801 acgtacgcgt gatcacctcg ggggcgagcg acgatccgcc cataccgccg agcacgatgt
    13861 ggtcgacgcc gtcctcgcgc agcttgtccc gcagcgcgac gatgtcggcc accatcggcc
    13921 gtgagacgga caccgcctcg acccagccca gtcgcttggc ggattcctct tcggcatccg
    13981 gcccccacag ggtctcgtca ccaccggtga tgcggctcgc gacgaggtcg ctgaccagct
    14041 gaggcactac gcggtcgacg gcctcggccg cagcgccgga gacggcgatc ttgacgctca
    14101 cttggccgcc tcgagagcgg cagacacggt gtcgagcagt tcgttccagg agacgatgaa
    14161 cttctcgacg ccctccttct cgagaagcgc cgtcacctcg tcgtaggaga ttccgagggc
    14221 cgccagcgcg tcgagcgtgg cattcgcctc ggcgtaggag ccggtgacct gatcaccctc
    14281 gatgacgccg tgatcgaaag tcgcctccag cgtcttctcg ggcatcgtgt tcacggtgtc
    14341 ggccaccgcg agttcggtca cgtacagggt gtccggcagg ctcgggtcct tcacaccggt
    14401 cgacgcccag agcggacgct gcttgttggc tcccgcggcg aggagcacct tggcgcgctc
    14461 cgaggcgaat gcctgctcgt agacctcgta tgcgaggcgg gcgttcgcga tgccggcttt
    14521 gctcttgagc gccttggcct cgtcggtgcc gatcgcgtcg agacgcttgt cgatctcggt
    14581 gtccacgcgg gacacgaaga acgaggccac ggagtggatg gtggagatgt cgatgcccgc
    14641 tgccttcgcg gtctcgagac cggtgagata cgcgttgatg acctcgcggt ggcgctcgag
    14701 gctgaagatc agggtcacgt tgacgctgat acccgcaccg atagtcgcgg cgatcgcctc
    14761 gagaccctcg atggtcgcgg ggatcttgat gagcgcgttc tcgcggtcga ccttctccca
    14821 gagctgctta gcctgagcga tcgtgcccgc agcgtcgtga gcaagtcccg gctcgacctc
    14881 gatcgagacg cggccgtcga agccggcagt cgcatcgtag atcgggcgga agatgtcgca
    14941 ggccgccgcg acatcatcgg tggtgatctc gaagactgcg tcggtgaccg cagcgccggc
    15001 cttcgccaga gctcccaccg cggcgtcgta cgcctcaccc ttcgcgagtg cgctggcgaa
    15061 gatggtcggg ttggtcgtga cgccgacgac attgcgctcg gagatcagct tctgcagtcc
    15121 gcccgaggcg atgcgttcac gggagaggtc gtcgagccag atgctgactc cgagggcgga
    15181 cagggctgcg gtgggggatt cggtgctcat tgcttcttct tcctgaggcc ggcctactgg
    15241 gcggccaacg attccttggc tgcggccacg gcggcctcgg tggtgattcc gaattcacgg
    15301 aacagagtct tgtagtcggc ggatgctccg aagtgctcga tcgagacgct gcggccgcga
    15361 tcaccgacgt aggggcgcca ggtgaggttg atccccgcct cgatggagac gcgagcggtg
    15421 acggaggccg gaagtactga ctcgcggtac gcggcatcct gctcctcgaa ccactcgagg
    15481 ctcggtgctg agacgacgcg agcgttgatg ccctcggcag ccagcacgcc tcgtgcctcg
    15541 acggcgatct gcacttccga gccggtcgca atgaggataa cgtcggggga gccgttgggg
    15601 gcctcggcga gcacgtaggc gccccgcgag acgttgctcg cggcggcgag agtgtcaccg
    15661 gatgccgcgc caggaccgcg ctcgaagacg ggcaggttct gacggctcag cgcaatacct
    15721 gccgggccct tgcggcgctc gaggatcgtc ttccacgccc acgcgacctc gttcgcatcg
    15781 gcgggccgca cgacgtcgaa gccggggatc gcgcggaggg tggcgagctg ctcgatcggc
    15841 tggtgggtcg gtccgtcctc accgagggcg accgagtcat gcgtccacac gaagatcgac
    15901 ggcaccgcca tgagagctgc gagacgaacc gcgggccgca tgtagtcgct gaagatcagg
    15961 aaggtgccgc cgaaggcccg cgtggggccg tgcagcacga tgccgttgat gatcgccgcc
    16021 atcgcgtgtt cgcggatgcc gaaatgcagc acacggccgt agtcgttgcc cgtccactcg
    16081 tgcgtggagt gcacgctcgg gatgaacgac gcggcgccct cgatggtggt gaggttcgac
    16141 tcggcgaggt cggctgagcc gccccagagc tcggggatga cggcacccag cgcgccgagc
    16201 accttgccgc tcgcggcgcg agtggagaca tccttgcccg cctcgaagac gggcagtgct
    16261 gcctcgacac catccggcag cgcgccggtc tcgagccggt cgaacagcgc cttgcgctcg
    16321 gggttcgccg cggcccaagc gtcaaaaccg acctgccact cggcatgggc ggccttgccg
    16381 cgctcgacgg ccttgcgggt gtgctcgatg acctcgtcgg cgacctcgaa ggtctgctcc
    16441 gggtcgaagc ccaacacgcg cttggtcgcg gcgagctcgt cggcgcccag ggcagagccg
    16501 tggatcttgc ccgagttctg cttgtgcggt gccggccagc cgatgatcgt gcgcaggatg
    16561 atgagcgacg gcttgtcgat gacgcccttc gccgcctcga tcgccgcgtt caactcggcg
    16621 acgtcctcgg tgtagacacc ggtcttcttc cagtcgacga cctgcacgtg ccagtggtag
    16681 gcctcgtagc gggccttgac gtcctccgtg aacgcgatgt tggtgtcatc ctcgatcgag
    16741 atctggttcg agtcgtagat cgcgatgaga ttgccgagct gctggtgacc ggcgagcgag
    16801 gatgcctccg ccgatacgcc ctcctgcagg tcgccgtcgc ccgcgatgac gtagatgaag
    16861 tggtcgaacg ggctcgtgcc cgccggtgcg tcggggtcga agagcccgcg ctcatagcgg
    16921 ctcgcgtagg cgaagccgac ggccgacgag atgccttgac cgaggggacc cgtggtgatc
    16981 tccacacccg cggtgtggcc gtactccggg tgtccgggag ttttcgagcc ccaggtgcga
    17041 agcgccttga tgtcgtcgag ttcgaggccg tagccgccga agtagagctg gatgtactgg
    17101 gtgagcgaac tgtggcccac cgacaggatg aagcggtcgc gaccgatcca cgcgttgtcg
    17161 ctggggtcgc ggcgcatgac cttctggaac agaaggtacg cggcgggggc gaggctcata
    17221 gccgtgcccg ggtggccgtt gccgaccttt tcgacggcat cggcagcgag aat
//
LOCUS       DAOSOU010000018        15797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3733_length_15797_cov_6.241837, whole genome shotgun sequence.
ACCESSION   DAOSOU010000018 DAOSOU010000000
VERSION     DAOSOU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 15797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15797
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3733_length_15797_cov_6.241837"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..585)
                     /locus_tag="PLW15_01875"
     CDS             complement(1..585)
                     /locus_tag="PLW15_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33180.1"
                     /translation="MLSRIAAASAALVAVALLSGCTSNPVAPQPEPTASAGSSAPATA
                     GGRLPTCAEVTDAIGSLVGSLAFNATTSDAQTAPEDYAQRVCVYETEDSVTQLGVTIA
                     AIPFQQAEIDSYATLPNAIADDRLAQYGAVIQTLGPDDAVDGVLDSALYLFDTTYSIT
                     IQGVSTGDPIAVSLPQLTVSAAIDGAFAVRALLG"
     gene            712..2169
                     /locus_tag="PLW15_01880"
     CDS             712..2169
                     /locus_tag="PLW15_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOT33181.1"
                     /translation="MSSETTSTETRIETDSLGSMPVPALAYWGIHTARALENFPISRR
                     ALSVYPDLIIALARVKQAAARANLELGVLEPEKAAVIDQVCEEIVGGALHRQFCVGVI
                     QGGAGTSTNMNANEVIANRGLEILGHGRGEYQFLHPIDDVNRSQSTNDVYPTAIKLAM
                     VMGVQRLLAEHERLTKSFAAKGREFNSILKIGRTQMQDAVPMTLGQEFSGFAHTLQED
                     HDRLSETIPWLNEINMGATAIGTGITADPRYADAVRRHLIEVTGIEMETAPDLIEATS
                     DAGIFMTLSGILKRSAVKLSKICNDLRLLSSGPQAGFGEIFLPARQAGSSIMPGKVNP
                     VIPEVVNQVAFSVIGADATVTAAAEAGQLQLNAFEPVIAHSILQSLMWMTNACYTLRV
                     NCIDGITANVERLAMQVETSVGVVTALTPYIGYAAAATLAHTALTTNASIKDLVVEAG
                     LMTEEKVSQVLSPERLSGMVPLTGPLTLPIVPPKP"
     gene            complement(2166..2672)
                     /locus_tag="PLW15_01885"
     CDS             complement(2166..2672)
                     /locus_tag="PLW15_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOT33182.1"
                     /translation="MNPRLIMLGLGIAVGYVLGTRDGRERYDAMKKKVTELWEDPRVA
                     RARKDVEDYARAQAPIIRDRAEAAAKAAPGVARDVAEKVSTTAKDVAGQVSTTAKDVA
                     SKATATAKDVAEKASATAKDVADRAKDVAADLRERGENLVDSAVATAGEARDAALDVL
                     DDEDDAKA"
     gene            complement(2713..>4395)
                     /gene="ligD"
                     /locus_tag="PLW15_01890"
     CDS             complement(2713..>4395)
                     /gene="ligD"
                     /locus_tag="PLW15_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOT33183.1"
                     /translation="ELASKDLAHLDYREVIKRVEKKGDILENLTAGHLASLEPTPQRM
                     AGFIAATPAPGGPDDRLATYRSMRDGSKTPEPVPVDAPATSQGNSFVIQEHKARRLHY
                     DFRLEHDGVLVSWAIPKGVPTDLSGNHLAVQTEDHPLAYGAFEGTIPAGQYGAGEVTI
                     WDRGTYDLEKWRDGKEVIATLHGEKHGSHRYALIHTGGRDGDSENNWLIHLMKSVEEQ
                     PVRTARADSRRKVIGGTRPVRRASDRLPAPMLASPGTAGELGDTGQWAFEMKWDGIRA
                     LARIDDGQVQVISRNGIDVSATYPELAELAEIVSGDALIDGEIVALNKAGRPDFALLQ
                     KRMNLNKKSDVDPAAAATPVTFMAFDLLELRGRSLIGEEYTERRRELEEALAPRGAIE
                     VPPAFEGSIEEAMRTSRTLGLEGVIAKRRASTYSVGKRTRSWIKLKHHLAQEVVIGAW
                     RPGSGARSHRVGSLLMGIPGEDGLRYVGRVSSGISEKEFDLLVSKFRRMERVTSPFAE
                     LPAADAADAHYISPTLVGEVEFAEWTPTGKLRHPVWRGWRPDKPVADVRAES"
     assembly_gap    4396..4495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4496..5025)
                     /locus_tag="PLW15_01895"
     CDS             complement(<4496..5025)
                     /locus_tag="PLW15_01895"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490708.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single-strand break in
                     duplex DNA and has been shown to have polymerase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOT33184.1"
                     /translation="MAADEQTVTIDGHRLKLTNLGKVLYPETGTTKADVLDYYARVSD
                     ALIRHAANRPATRKRWVHGVGTDEEPGEMFFQKNLDERSTPQWVLRREIQHSDHVNTY
                     PLVNDLATLTWLGQIAALEIHVPQWQFGRTGVRKNPDRMVLDLDPGPGAGLQECAEVA
                     RLARVILTGMGLDPMPV"
     gene            5085..5921
                     /locus_tag="PLW15_01900"
     CDS             5085..5921
                     /locus_tag="PLW15_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOT33185.1"
                     /translation="MRSIWKGAITFGLVNVPVKVYSATEDHDVSLHQVHDADGGRIRY
                     QRRCEICGNVVEYANIAKAFDDGERTVVLTEADLASLPAERSKEIDVVEFVPSDQVDP
                     IMFEKSYYLEPDSRSVKAYALLRRTLEETDRTAIVTFALRQKTRLGALRVRGDVLMLQ
                     ALLWDDEVREARFPALDEKVSISAKELEMSEALVKSFESDFSPEKFTDDFQDQLRELI
                     EAKLDKGDAVDTAETFGEKADDDNGGEVLDLMEALRRSVERNRAAAKPAPKKSPKKSA
                     SA"
     gene            complement(5918..6397)
                     /locus_tag="PLW15_01905"
     CDS             complement(5918..6397)
                     /locus_tag="PLW15_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HOT33186.1"
                     /translation="MTSLPADAPEIETEHDRLERNWSELLQELRVIQTGTQILTGFLL
                     TVAFAQRFVDLDEYQVTAYLTLVIVAGLTTALGLAPVSLHRTLFRRRAKETIVRITNR
                     ILQVTLFGVALTLSGTIMLIFDVVVGRGPGVVAGVVALILISATWVLLPAAARRHLN"
     gene            complement(6394..7863)
                     /locus_tag="PLW15_01910"
     CDS             complement(6394..7863)
                     /locus_tag="PLW15_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOT33187.1"
                     /translation="MGATEARFDERFAERYRALSSRDTRFDGQFIAGVHSTGIYCRPS
                     CPAMTPHAKNVSFYRTAAAAHEDGLRACKRCQPDAVPGSPDWNLSDDLASRAMRLISD
                     GIVEREGVTGLAARLGYTSRHLTRVLATELGAGPLALARAHRAQTARTLLASTELSIA
                     DVAFASGFSSIRQFNDTILSVYETTPSGLRALARRGRARVAATDATSITLRLPARAPF
                     DGPGLMRFFADHAVPGLESGDTETFERAVRLPGGTVHLSLALDGDSGVRCTASLESIA
                     DVATLVARVRRLCDLDADSHAIDGALSADAVLAPLVVAVPGIRLPGALDPEEALMRTL
                     VGQQISVAAARTVLGRITAELGTDGLFPTATQLAEHGETVLRGPASRVTTILGAARAL
                     ASGELVLDVGMPADEFTARLVALPGIGPWTAGYLAMRVLGNPDVLLTSDLVIRQSAGA
                     LGLPSSPGALAEHGSRWAPWRSYAGLHLWRSRPTGTIRA"
     gene            7939..9288
                     /locus_tag="PLW15_01915"
     CDS             7939..9288
                     /locus_tag="PLW15_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT33188.1"
                     /translation="MADVVQLLSQALGDVVSVDAHQLDAARYDKSGHAAPAAPLAVVT
                     ARSIDDVQATMRIATETGTAVVTRGAGTGLAGGAIAGAGEIVLSTLGMDRLLEVSVDD
                     ELAVVQPGIINAELNRQLEPHGLWFAPDPASRAISTVGGNIATNAGGLLCAKYGVTRE
                     AVLGLKVVLADGRLIELGHRTVKGVTGLDFTALMIGSEGTLGVIVEATVKVRRLVAGV
                     VPTISATFATVDDAARASAVITASGLHPSIMELIGDSAMAAICAHLGIEHPGGSQLII
                     QTDGPGAEIEAETIAAIIASLGGAVELSIDAEDAARLFSIRRSAHAAISTLGTVLIED
                     VCVPRSALPAMFAAIHEIEQKYSITIPVVAHAADGNLHPNFVFQGDEVPQQIWSAADE
                     MFLAALRLGGTLTGEHGIGILKKRWLRDELGDDQFDLQQKVKAVFDPLGILNPGKVF"
     gene            9324..9875
                     /locus_tag="PLW15_01920"
     CDS             9324..9875
                     /locus_tag="PLW15_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33189.1"
                     /translation="MTFLPITTYESLDATARAASDRQLELHGGRITNMKATLLSHVPS
                     FDAYMQWYTLRDALVPFIGERAVSLFSYAISDENDCLVCSVFFRKILIDNGEDPDHPN
                     VTEAEQLLIDFGRAIARTPSAIEPEFYARLESTFNPQLRVLLVAFAGQMVATNLFVTV
                     GKVPLDEVLYDYRKPGDERTGGD"
     gene            9853..10680
                     /locus_tag="PLW15_01925"
     CDS             9853..10680
                     /locus_tag="PLW15_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HOT33190.1"
                     /translation="MSAPGETDRPFAGRVAFITGAAHGQGRAVALALAESGADIVAFD
                     VAAKIEYPAYAQGSSAELDSLVAEVHALGRRALAFVGDVRDSAAIQQAVDAAVAEFGR
                     IDVLFNNAGIVAYAAADEMTELEWDAMIDVNLKGPFLVARSVIPIMKAQRSGVIINNS
                     SVMGLRGGNRLSHYVASKHGLTGLTKAWAIELAPWGIRVVSIHPTGVNTPMNNGLAEL
                     EGATAIEIAERSAGNLLPGVPWIEASDVAELVLYLASDRARYATGAQFTLDAGLLTR"
     gene            10719..11537
                     /locus_tag="PLW15_01930"
     CDS             10719..11537
                     /locus_tag="PLW15_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33191.1"
                     /translation="MELNWMPATVALAVWLVALSLQLADLGTARFDIRRLNRGTRTLV
                     RLHPVTWVVPLAALSLVALGFAIEYGQRLLFDEGEPVMAMVVALAIVVLLVAGWVVIT
                     SAATSPAADSYRAVRDELVDLRGSRVQQEWLDGLRERLAVIDETTARTPAPPIVSWRA
                     AAGWVVRRPQRTLPVISAVLLLVLVIVAAVEDPGHEWVVVAAIGAVLVSMACAVLGAR
                     ASLVLLAAVRETQLQYRREAEQLLIEAEKTSRKPVAGLGERVTRALQILREQQS"
     gene            11577..12806
                     /locus_tag="PLW15_01935"
     CDS             11577..12806
                     /locus_tag="PLW15_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOT33192.1"
                     /translation="MTNPTAATPDHTASAGITGAAPATAPAAVAQSAVTAPAGLAAPV
                     QRSSAVVALAIIGIIVLAVIALFVTVYLISGLGVSGFALGGVMALVPLAIVFFGVRWI
                     DRWEPEPRAAVVFAFLWGAGVAVLIALLVGAEIDNVINSMGGPGPGYDFFAAAIQAPI
                     VEETAKGLGVLILFWAARRHFDGPVDGLVYAAWVAGGFAFTENILYFGSELLNPQGAP
                     LEIFLIRGIMSPFAHVMFTACTGLALGFAARRTGAFGAIGFFLLGLIPAIFLHALWNG
                     ALFFVSDFFAYYAIVQFPLFLGAVFLVLYLRRQEAKLTHDRLAEYAAVGWFSPEELPA
                     LATRPGRRQAITWANRNGKGAEMKKYIRDATRLAFARQRIITGRHRTAAEADETALLA
                     SIVESRKNLTGLGTATA"
     gene            complement(12861..13937)
                     /locus_tag="PLW15_01940"
     CDS             complement(12861..13937)
                     /locus_tag="PLW15_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33193.1"
                     /translation="MARSVDYTALTAPVTSEQVRTFRQQALRDPAYSSQFGVARVVVL
                     VFVFVVIGFLFLGFAGVIVSMVASSAAQGLTITTAVSLLFPLLFVAAFAIIVVIAVRG
                     ALGGGGRWERWYRLTTFAAANGLIFSPVDADPTYPGAIFISGRSRVALDHLRTPAGRF
                     FDYGNYRYVTGSGKNSTTHNWGFVALSLDRALPHMVLDSRSNNGLFGGTNLPTVFSKD
                     QVLSLEGDFDTHFTLYCPREYERDALYVFTPDLMALLIDEAAPFDVEIVDSWMFVYSA
                     TVFDLRNPAAHQRILRIVDTVGAKTLSRTDLYADERVGSPAANIVAPEGRRLRRGVSV
                     VAVITVIVFGAIWAWSFLADALGI"
     gene            14113..>15797
                     /locus_tag="PLW15_01945"
     CDS             14113..>15797
                     /locus_tag="PLW15_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT33194.1"
                     /translation="MPSGELDTERAYVATLYARLDALREEAQQQLEVVRRSNPGGTHQ
                     NRSERDAFARIYEDRVLQLRQIDERLAFGRLEMKSDGAEPVLHYIGRIGLRDEEQKPL
                     LLDWRVPQARAFYQATAATPLGARARRHLQSQGREIVRIDDEIFDPDLLDGDVSGLQG
                     EAALMATLTAQRTGRMSDIVATIQAEQDAIIRSELGGTLVVQGGPGTGKTAVALHRAA
                     YLLYSHRDRLAASGVLIVGPSRSFLQYIEAVLPSLGETGVVLASVGQLFPGMDVSAED
                     APEVAALKGSLEMAQLLSRAVRSRQVVPTEPQVLDINGELLTVPPQLIHAAMQRAWET
                     KKPHNLARVTFNKAAISALSRLLADQLRSHGNTIEDSDQAWLREDLRTSHDVKVALNT
                     AWLPLTPQKLLQDLYARPQWLASLTPRWTAEQRELLARDRSAEFTVSDVALLDEAAEL
                     LGESDDLADAHKSERKQQRKRDIENAEQAIRNMGVEGLVDAATLAEGFEAAVERGTTA
                     EIAAADREWAYGHVVVDEAQELSPMQWRLLMRRNPLKSFTIVGDVAQASAAAG"
BASE COUNT         2522 a         5256 c         5362 g         2557 t
ORIGIN      
        1 tcagcccagc agcgcccgaa cggcgaatgc accgtcgatg gctgccgaca ccgtcagttg
       61 cggcaggctc acggcaatcg ggtcaccggt ggagaccccc tggatagtga tcgagtaggt
      121 cgtatcgaac aggtacagcg ccgagtccag cacgccgtcc acagcatcgt cggggccgag
      181 agtctggatg acggcgccgt actgggccaa ccggtcatcc gcgatcgcgt tgggcagggt
      241 cgcgtaactg tcgatctcgg cctgttggaa cggaatcgcg gcgatcgtca ccccgagctg
      301 agtcaccgag tcctcggtct cgtacacgca gacccgctgc gcgtagtcct ccggggcggt
      361 ctgcgcgtcg gaggtcgtcg cgttgaaggc gagtgacccg accagcgatc cgatggcgtc
      421 ggtaacctcg gcgcaggtgg gaaggcggcc gcccgcggtg gccggtgcgc tcgaacctgc
      481 gcttgccgtg ggctccggtt gcggggcgac agggttggac gtgcagccgc tgagaagcgc
      541 gacggcaacc agtgccgcgg atgccgcggc gattctcgaa agcatgctgt gacgataaca
      601 attcccgcct gtgaatgaga tcggcaggcg ggtcgaaggc atagagtggt tggtcgccgg
      661 tcaacgacga tcggcaggaa caacacaacg ccacaagcgt gaagggccat catgtccagt
      721 gaaaccacgt ccaccgaaac gcggatcgag accgactccc tcgggtcgat gccagtccca
      781 gcccttgcat actggggcat ccacacggct cgcgcgctcg agaacttccc gatcagccgc
      841 cgcgccctct cggtgtatcc cgacctcatc atcgccctcg cgcgagtcaa gcaggccgct
      901 gccagggcca acctcgaact cggggttctc gaacccgaga aggcggcggt gatcgaccag
      961 gtctgcgagg agatcgtggg cggggcgctg caccgccagt tctgcgttgg cgtcatccag
     1021 ggcggtgcgg gaacctccac gaacatgaac gcgaacgagg tcatcgcgaa ccgcggtctc
     1081 gagattctcg ggcacggcag gggggagtac cagttcctcc acccgatcga cgacgtcaat
     1141 cgcagccaga gcaccaacga cgtctacccc acggcgatca agctggccat ggtcatgggt
     1201 gtgcagcgac tgctcgccga gcacgagcgc ctcaccaagt cgttcgccgc gaaggggcgc
     1261 gagttcaaca gcatcctcaa gatcggtcgc acccagatgc aggatgccgt gcccatgacc
     1321 cttgggcagg agttctccgg gttcgcgcac acgctgcagg aggaccacga ccggctctcg
     1381 gagacgatcc cgtggctcaa cgagatcaac atgggtgcga cagccatcgg caccggcatc
     1441 accgccgacc cgcgctacgc cgacgcggtg cgccgccacc tgatcgaggt caccggcatc
     1501 gagatggaga ctgctcccga cctgatcgag gcgacatctg atgcgggcat cttcatgacc
     1561 ttgagcggca tcctcaagcg ctccgcggtc aagctctcga agatctgcaa cgacctgcga
     1621 ctactctcca gcggacccca ggccggattc ggcgagatct tcctgccggc acgtcaggcg
     1681 gggtcgtcga tcatgccggg caaggtcaat ccggtcatcc ccgaggtcgt caaccaggtc
     1741 gcgttcagcg tcatcggggc ggatgccacg gtcaccgccg cggcggaagc cgggcagttg
     1801 cagctcaacg ccttcgagcc ggtgatcgcg cactcgatcc tgcagtcgct catgtggatg
     1861 accaacgcct gctacacgtt gcgcgtcaac tgcatcgatg gcatcaccgc caatgtcgaa
     1921 cgactcgcca tgcaggtgga gacctcggtg ggcgtcgtca ccgccctcac gccgtacatc
     1981 ggctacgctg cggcggcgac cctcgcccac acggctctga cgaccaacgc gtcgatcaag
     2041 gatctggtgg tcgaggctgg cctcatgacc gaggagaagg tgtcacaggt gctctcgccc
     2101 gagcgtctga gcggcatggt gccgctgacg ggaccgctca cgctgccgat cgtgccgccg
     2161 aaaccctagg ccttcgcgtc gtcctcgtcg tccagcacgt cgagggcggc gtccctggcc
     2221 tcgccggcgg tcgcgacggc ggagtcgaca aggttctcgc cgcgctcccg aaggtcggca
     2281 gcgacatcct tggccctgtc ggcaacgtcc ttggcggtgg cgcttgcctt ctccgcgacg
     2341 tccttcgctg tcgcggtggc cttgctcgcc acatccttgg cggtggtcga cacctgaccc
     2401 gcgacatcct tggcggtcgt actgaccttc tcggcgacat ccctggcgac tcccggggcg
     2461 gccttggcgg ctgcctcggc gcgatcgcgg atgatcggcg cctgagctcg ggcgtagtcc
     2521 tcgacatcct tgcgcgccct ggcgactcgc gggtcctccc agagttcggt caccttcttc
     2581 ttcatggcgt cgtagcgctc gcgcccatcc ctggtgccga gcacatagcc gaccgcaatt
     2641 ccgagtccga gcatgatcag tctggggttc atacctgaca gcctaggttg cccgcccagc
     2701 actggccacg gcctagcttt cggcgcgaac gtctgctacc ggcttgtcgg gccgccaccc
     2761 gcgccacacc ggatgccgca gctttccggt cggcgtccac tcggcgaatt ccacctcgcc
     2821 caccagcgtg ggggagatgt agtgcgcgtc ggcggcgtcc gcggccggca actcggcgaa
     2881 cggactggtc acccgctcca tccggcggaa cttcgatacc agaagatcga attccttctc
     2941 gctgatcccc gacgagacac gtccgacata gcgcagcccg tcctcgccgg ggatacccat
     3001 gagcagcgag ccgactcggt gcgaccgtgc gccagaaccg ggccgccatg cgccgatgac
     3061 gacctcctgc gcgaggtggt gcttgagctt gatccacgag cgcgtacgtt tgcccaccga
     3121 gtaggtgctg gcccgccgct tggcgatcac gccttcgagg ccgagagtgc ggctcgtgcg
     3181 catggcctcc tcgatagaac cctcgaacgc gggcggcacc tcgatcgcgc cgcgcggggc
     3241 aagcgcctcc tccagttcgc gtcgacgttc ggtgtactcc tcgccgatga ggctccgccc
     3301 gcgcagctcg agaagatcga atgccatgaa cgtcacgggc gttgcggccg cggccggatc
     3361 gacgtcggac ttcttgttca ggttcatgcg cttctgcagc agcgcgaagt cggggcgccc
     3421 cgccttgttc agcgcgacga tctcaccgtc gatgagggca tccccggaca cgatctccgc
     3481 cagctcggcg agctcgggat aggtcgccga cacgtcgatt ccgttgcggc tgatcacctg
     3541 cacctggccg tcgtcgattc tggccagggc acggatgccg tcccacttca tctcgaacgc
     3601 ccactgaccg gtgtccccga gttcgcctgc cgtgccggga ctcgccagca tcggtgctgg
     3661 cagtcgatca gacgcgcgcc gcaccggacg ggtgccgccg atcaccttgc gtcgggaatc
     3721 agccctggcg gtgcgcaccg gctgctcctc gacggacttc atgaggtgga tgagccaatt
     3781 gttctcgctg tcaccgtccc tgccgccggt gtggatgagc gcgtaccggt gcgacccgtg
     3841 cttctcgccg tgcagcgtcg cgatcacctc cttgccgtcg cgccacttct ccaggtcgta
     3901 ggttccgcgg tcccagatgg tcacctcgcc tgcgccgtac tgcccggccg ggatggttcc
     3961 ttcgaaggcg ccgtaggcga gcggatgatc ctccgtctgc acagcgaggt ggttgccgct
     4021 cagatcggtg ggaaccccct tcgggatcgc ccagctcacc agcacgccgt cgtgttcgag
     4081 ccggaagtcg tagtgcaacc gccgcgcctt gtgctcctgg atgacgaacg agttgccctg
     4141 cgaggtcgcg ggggcgtcca cgggcacggg ttcgggagtc ttggacccgt cacgcatgct
     4201 gcggtaggtg gcgaggcggt catccgggcc accgggtgct ggtgtcgcag cgatgaagcc
     4261 ggccatccgc tgcggggtcg gttcgagtga ggcgaggtgg ccggccgtca ggttctcgag
     4321 gatgtcgccc ttcttctcga cgcgcttgat cacctcgcgg tagtcgaggt gcgccagatc
     4381 cttgctcgcg agctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccgg
     4501 catcgggtcg agtcccatgc cggtcaggat gacgcgagcc agacgcgcca cctcggcgca
     4561 ctcctgcagc ccggctcccg gtcccgggtc gaggtccagc accatgcggt cgggattctt
     4621 gcgcacgccg gtgcgaccga actgccactg cggaacatgg atctcgagtg cggcgatctg
     4681 cccgagccag gtgagggtgg cgaggtcgtt gaccagcgga taggtgttga cgtggtcgga
     4741 gtgctggatc tcccggcgca gcacccactg cggcgtcgag cgctcgtcga ggttcttctg
     4801 gaagaacatc tcgccgggtt cctcgtcggt tcccacgccg tgcacccagc gcttgcgggt
     4861 cgccgggcgg ttggcggcat gccggatgag ggcgtcggag acccgggcgt agtagtcgag
     4921 cacgtcagcc ttggtggtgc ccgtctcggg gtagagcacc ttgcccaggt tggtgagctt
     4981 gaggcgatga ccgtcgatcg tcacggtctg ctcgtcggct gccatgactc catcatgggc
     5041 ggtcgggggt gacagggcta ggccggtggg ggagacttga cggcatgcga tcgatctgga
     5101 aaggcgccat caccttcggg ctcgtcaacg tgcccgtcaa ggtctacagc gccaccgagg
     5161 accacgacgt gtccctgcac caggtgcatg acgccgacgg cggacgcatc cggtaccagc
     5221 gtcgctgcga gatctgcggc aatgtcgtcg aatacgcgaa catcgcgaag gctttcgacg
     5281 acggtgagcg caccgtcgtg ctcaccgagg ccgacctggc gtcacttccc gccgagcggt
     5341 cgaaggagat cgacgtcgtc gagttcgtgc cgagcgatca ggtcgacccg atcatgttcg
     5401 agaagagcta ctacctcgag cccgactcca gatcggtgaa ggcctacgcg ctgctgcgtc
     5461 gcaccctcga ggagaccgat cgcacggcga ttgtgacctt cgcgcttcgg cagaagaccc
     5521 gtctcggcgc gctgcgggtg cgcggcgatg tgctgatgct gcaggcactt ctctgggacg
     5581 acgaggttcg cgaggcgcgg ttcccggcgc tcgacgagaa ggtgtcgatc tcagcgaagg
     5641 agctggagat gagcgaggcg ctggtcaaga gcttcgagtc ggacttctcg cccgagaagt
     5701 tcaccgacga cttccaggac cagctgcgtg agctcatcga ggccaaactc gacaagggcg
     5761 atgccgtcga caccgccgag acgttcggcg agaaggccga cgacgacaac ggcggtgagg
     5821 tgctcgacct catggaggcc ctgcggcgca gcgtcgagcg caaccgcgca gcggcgaagc
     5881 ccgcgcccaa gaagagcccc aagaagtccg cgagcgccta gttcaggtgc cgccgggccg
     5941 cggcgggaag cagcacccag gtcgcgctga tcaggatgag cgccaccacc ccggcgacga
     6001 cgcccgggcc acggccgacc acgacatcga agatcagcat gatcgtgccc gacagagtca
     6061 gcgcgacccc gaacagcgtc acctgcagga ttcggttggt gatgcgcacg atggtctcct
     6121 tcgcgcgacg gcggaacagc gtgcggtgaa gactcacggg tgcgaggccg agcgccgtcg
     6181 tgaggccggc gacgatcacg agggtgaggt aggccgtcac ctggtactcg tcgaggtcga
     6241 cgaatcgctg cgcgaacgcg acggtgagca ggaagcccgt cagaatctga gtaccggtct
     6301 ggatgacccg cagctcctgc agcaactcgc tccagttgcg ctccagacga tcgtgttcgg
     6361 tctcgatctc gggggcgtcc gcgggaaggc tggtcatgcc cgaatcgtac cggtcgggcg
     6421 tgaacgccag aggtgcaggc ccgcatagct gcgccacggg gcccaccgcg acccgtgctc
     6481 ggcgagggct cctggcgacg agggcaggcc cagagcgccg gcgctctgcc tgatcacgag
     6541 gtcgctggtc aggagcacgt ccgggttgcc cagcactctc atcgcaaggt atcccgcggt
     6601 ccaggggccg atcccgggca gtgccacgag ccgcgcggtg aactcgtcgg cgggcatccc
     6661 gacatcgagc acgagctcgc cgctggcgag cgctcgggct gctccgagga tcgtggtcac
     6721 gcgtgatgcc ggtcccctca gcaccgtctc gccgtgctcg gcgagctgcg tggccgtcgg
     6781 gaacaggccg tcggtgccca actcggcggt gatgcggccg agcaccgtgc gcgctgccgc
     6841 caccgagatc tgctggccga ccagagtgcg catgagcgcc tcctcggggt ccagggcgcc
     6901 cggcagacgg atgcccggca ccgccaccac gaggggagcg agcacggcat ccgccgacag
     6961 cgcgccgtcg atcgcgtggg agtcggcgtc caggtcgcag aggcgacgga cccgcgcgac
     7021 gagggtcgcg acatccgcga tgctctcgag gcttgccgtg catcgcacgc ctgagtcgcc
     7081 gtcgagggcg aggctcaggt ggaccgttcc cccgggaagt cgcacagcgc gctcgaaggt
     7141 ctcggtatcg cccgactcga gtccgggcac ggcgtggtcg gcgaagaagc gcatgagccc
     7201 cggcccgtcg aatggggctc gggccggcag tctgagcgtg atgctggtgg cgtcagtcgc
     7261 ggccacccgg gcccgccctc gtcgagcgag ggcacgcaga ccactcgggg tggtctcgta
     7321 gacgctcagg atcgtgtcgt tgaactggcg aatgctggag aagccgctcg cgaacgcgac
     7381 gtcggcgatg gatagctcgg tggaggcgag cagggtgcgc gccgtctgtg cacggtgcgc
     7441 ccgggcgagc gcgagcgggc cggcaccgag ctcggttgcg agcaccctcg tgaggtggcg
     7501 ggaggtgtag ccgaggcggg ccgcgagccc cgtgacgccc tcgcgctcga cgattccgtc
     7561 ggagatcagc cgcatggctc gcgaggcgag gtcatcgctc aggttccagt cgggggagcc
     7621 gggcacggcg tcgggttggc agcgtttgca ggcccgcaga ccgtcctcgt gggccgccgc
     7681 ggccgtgcgg tagaagctca cgttcttggc gtggggcgtc atggcggggc agctgggtcg
     7741 acagtagatt cccgtggagt gcacgcccgc gatgaactgg ccgtcgaaac gcgtgtcgcg
     7801 cgaggagagc gcgcggtagc gctcagcgaa tcgctcgtcg aatcgtgcct cggtagcgcc
     7861 catgagtatc agggtgccac ggcgccacgc ccttcactag cgggattcgg acatggccga
     7921 agactctaga gtggatccat ggccgatgtc gttcagcttc tctcgcaggc tctcggtgac
     7981 gtcgtctccg ttgatgccca ccagctggat gctgcgcgat acgacaaatc cggacacgcg
     8041 gcaccagcgg ccccgctggc ggtggtgacc gcgcgctcga tcgacgacgt gcaggccacg
     8101 atgcgcatcg cgaccgagac gggcacggcc gtcgtgacgc gtggtgccgg caccgggctc
     8161 gccgggggag cgatcgccgg ggctggcgag atcgtgctct cgacgctggg catggatcgt
     8221 ctgctcgagg tgtcggtcga tgacgagctt gcggtggtgc agccgggcat catcaatgcc
     8281 gagctcaatc ggcagctcga gccccacggc ctctggtttg cgcccgaccc ggcgagccgc
     8341 gcgatctcca ccgttggcgg aaacatcgcc accaacgcgg gaggcctgct ctgcgccaag
     8401 tacggcgtga cccgcgaggc tgtgctcggc ctgaaggtcg tgcttgccga cggccgtctc
     8461 atcgaactcg ggcaccgcac agtgaagggc gtcacgggcc tcgacttcac tgctctcatg
     8521 atcggctccg agggcaccct cggggtcatc gtcgaggcga cagtcaaggt gcggcgtctg
     8581 gtcgcgggtg tggtgcccac catcagtgca accttcgcca cggtcgacga tgcggcacgc
     8641 gcgtcggcag tgatcacggc atccggactt catccctcga tcatggaact catcggcgac
     8701 agcgccatgg ctgcgatctg tgctcacctc gggatcgagc atcctggtgg ctcacaactg
     8761 atcattcaga ccgacggacc cggcgccgag atcgaggccg agaccatcgc cgccatcatc
     8821 gcctccctcg gtggcgcggt cgagctctcc atcgacgcgg aggatgccgc gcgactgttc
     8881 agcatccggc gttcggcaca cgccgccatc tccactctcg gcacggtgct catcgaggac
     8941 gtctgcgtgc cgcgcagcgc cctccccgcg atgttcgcgg cgatccacga gatcgagcag
     9001 aaatactcga tcacgatccc ggtcgtggcc cacgccgccg acggcaacct gcacccgaac
     9061 ttcgtgttcc agggtgacga ggtgccgcag cagatctggt cggccgccga cgagatgttc
     9121 ctcgcggccc tgcgcctcgg cggaacactc acgggcgagc acggcatcgg catcctcaag
     9181 aagcgctggc ttcgcgatga gctgggcgac gaccagttcg acctgcagca gaaggtgaag
     9241 gcggtgttcg acccgctcgg catcctcaat cccggcaagg tcttctgaga cccgtggcgc
     9301 gcgcggcgca gtagcctgtt cgcatgacgt tcctgcccat cacgacgtac gagagtctgg
     9361 atgcgacggc ccgggcggcc agtgaccgcc agctcgaatt gcacggcggt cgcatcacga
     9421 acatgaaggc gaccctgctc agccacgtcc ccagcttcga cgcgtacatg cagtggtaca
     9481 cgctgaggga cgccctggtg ccgttcatcg gcgagcgtgc cgtgtcgctg ttcagctatg
     9541 ccatctccga cgagaacgat tgcctggtct gctcggtctt cttccgcaag atcctcatcg
     9601 acaacggcga ggaccctgac caccccaacg tcaccgaggc cgaacagctg ctcatcgatt
     9661 tcgggcgtgc gatcgcccgc acgccgtcgg cgatcgagcc ggagttctat gcgcggctcg
     9721 agtcgacctt caacccgcaa ctgcgcgtgc ttctcgtggc cttcgccggg cagatggtgg
     9781 ccaccaacct gttcgtgacg gtgggcaagg tgccgctcga cgaggtgctc tacgactacc
     9841 ggaagccggg ggatgagcgc accgggggag actgaccggc cgttcgccgg gcgcgtcgcc
     9901 ttcatcacgg gagcggcgca cgggcagggc cgtgctgtcg cgctcgcgct cgcggagtcg
     9961 ggcgcggaca tcgtggcgtt cgacgtcgcg gcgaagatcg agtatcccgc ctacgctcag
    10021 ggatcgagcg ccgagctcga ctcgctcgtc gccgaggtgc acgcgctcgg gcgccgagcc
    10081 ctggccttcg ttggggatgt gcgggactcc gccgcgatcc agcaggccgt cgatgccgcc
    10141 gtcgccgagt tcgggcgcat cgacgtgctc ttcaacaacg cgggaatcgt cgcctacgcc
    10201 gccgccgacg agatgaccga gctcgagtgg gacgccatga tcgacgtgaa cctcaagggt
    10261 ccgttcctgg ttgcgcgcag cgtcatcccg atcatgaagg cccagcgcag cggggtgatc
    10321 atcaacaact cgagcgtcat gggactgcgc ggaggcaacc ggctgtcgca ctacgtcgcg
    10381 tccaagcacg ggctgaccgg gctcaccaag gcgtgggcga tcgagctggc gccgtggggc
    10441 atccgggtgg tgagcatcca ccccacgggc gtgaacaccc ccatgaacaa cgggctagcc
    10501 gagctcgagg gggcgacggc gatcgagatc gccgaacgca gcgccggcaa cctcctgccc
    10561 ggggtgccgt ggatcgaggc gtcggatgtc gccgagctcg tgctctacct cgccagcgat
    10621 cgggcgcgct acgccaccgg cgcccagttc accctcgacg cgggactgct cacccgctga
    10681 tccggacgag gtacccgagc accggtagtg tctcaagcgt ggaactgaac tggatgcccg
    10741 ccaccgttgc cctcgccgtg tggctcgtcg ccctgtccct ccaactggcc gacctcggca
    10801 ccgcccggtt cgacatccgg cgcctgaacc gaggaacccg cacgctggtt cgcctccacc
    10861 ccgtgacctg ggtcgtgccg ctcgcagcgc tgtcgcttgt cgccctcggg ttcgcgatcg
    10921 agtatgggca gcgcctcctg ttcgacgagg gcgagcccgt catggccatg gtcgtggcgc
    10981 tggcgatcgt ggtgcttctg gtcgctggct gggtcgtgat cacgagtgcg gcgacgagcc
    11041 ccgcggccga cagctaccgt gcggtgcgcg acgagctcgt cgacctgcgc ggcagccgcg
    11101 ttcagcagga atggctcgac gggctgcggg agcgcctcgc cgtcatcgac gagacgacgg
    11161 cgcgcacccc cgccccgccg atcgtgtcct ggcgtgctgc agcaggatgg gtcgtccgcc
    11221 gtccccagcg cacgcttccg gtgatctcgg cggttcttct tctcgtgctg gtcattgtcg
    11281 ccgccgtcga ggatcccggt cacgagtggg tggtggttgc ggcgatcggc gccgtgctgg
    11341 tgagcatggc gtgcgctgtg ctcggggcgc gggcatccct cgtgctgctg gcggcggttc
    11401 gggagacgca gcttcagtac cggcgcgagg ccgagcagtt gttgatcgag gcggagaaga
    11461 cctcgcgcaa gcccgtcgcc gggctgggcg agcgtgtcac gagagccctg cagatcctgc
    11521 gggagcagca gagttgatga ccgatcggac ccgtcgcagc cgctagtgtc gtgggcatga
    11581 caaaccccac tgcggccacg cccgaccaca ccgcgtcagc gggaatcacc ggcgcggcac
    11641 cggcaacagc gcccgccgct gtcgcccagt ctgctgtcac agcaccggcc gggctcgccg
    11701 ctcctgtgca gcgcagttcg gcggtggtgg cgctggcgat catcggcatc atcgtgctcg
    11761 ccgtgatcgc cctgttcgtg acggtctacc tcatcagcgg gctcggggtc agtgggttcg
    11821 cactcggcgg tgtcatggca ctggtgccgc tggccatcgt cttcttcggg gtgcgctgga
    11881 tcgaccggtg ggagcccgaa ccgagggcag cagtcgtgtt cgcctttctc tggggtgccg
    11941 gcgtcgccgt gctgatcgcc cttctcgtgg gtgccgagat cgacaacgtg atcaacagca
    12001 tgggtggccc cggccccggt tatgacttct tcgcggcggc cattcaggct ccgatcgtgg
    12061 aggagaccgc caagggtctc ggcgtcctga tcctgttctg ggccgcgcgc aggcacttcg
    12121 atggtcccgt cgatggactg gtctacgcgg cctgggtcgc gggcgggttc gcgttcaccg
    12181 agaacatcct ctacttcggt tcggagctgc tcaacccgca gggtgccccg ctcgagatct
    12241 tcctcatccg cggcatcatg tcgccgttcg cacacgtcat gttcaccgcc tgcaccggcc
    12301 tcgcgctcgg gttcgccgcc cgtcgcactg gcgcgttcgg tgccatcggg ttcttcctgc
    12361 tcgggctcat tccggcgatc ttcctgcacg cactctggaa cggtgccctg ttcttcgtct
    12421 ccgacttctt cgcgtactac gcgatcgtgc agttcccgct cttcctcggg gcggtgttcc
    12481 tcgttctcta cctgcgacgg caggaggcca agctcacgca cgaccgactc gccgagtacg
    12541 cggcggtcgg gtggttcagc ccggaagaac tgccggcgct ggcgacccgt ccagggcgtc
    12601 gccaggcgat cacgtgggcg aatcgcaacg gcaagggcgc tgagatgaag aagtacatcc
    12661 gggatgcgac gaggctcgcc ttcgcccgcc agcgcatcat caccggacgt caccgcaccg
    12721 cggccgaagc cgacgagacc gccctgctcg cctcgattgt ggagagccgc aagaacctca
    12781 cgggcctcgg aaccgctacg gcctgatcag ctcgtcgggt tgcgtgcgtc gtaacgaccc
    12841 gccgacgctt cggggaccgg tcagatcccc agcgcgtcgg cgaggaacga ccaggcccag
    12901 atggcaccga acacgatcac ggtgatcacg gcaaccaccg acactccgcg tcggagtcgg
    12961 cgtccctccg gggccacgat gttcgccgct ggactgccca cgcgctcatc ggcgtacagg
    13021 tcggtgcgcg agagcgtctt cgccccgacc gtgtccacga tccgcaggat gcgctggtgc
    13081 gctgcgggat tgcgcaggtc gaacacggtg gcggagtaga cgaacatcca gctgtcgacg
    13141 atctcgacat cgaacggcgc ggcctcatcg atcagcagag ccatgagatc gggggtgaac
    13201 acgtagaggg catcccgctc gtactcccgc gggcagtaca gggtgaagtg ggtgtcgaag
    13261 tcgccctcga gtgacagcac ctggtctttc gagaagaccg tcggcaggtt ggtgccgccg
    13321 aagagtccgt tgttggaccg ggagtcgagc accatgtgtg gcagagcgcg gtcgaggctc
    13381 agcgccacga agccccagtt gtgggtcgtc gagttcttgc cgctgccggt gacatagcgg
    13441 tagttgccgt agtcgaagaa ccgaccggcg ggggtgcgca ggtgatcgag cgcgacccgg
    13501 ctgcgtcccg agatgaagat cgcgcccggg taggtcgggt cggcgtcgac cggggagaag
    13561 atcagcccgt tggctgccgc gaaggtggtg agccggtacc accgttccca tcgaccgccc
    13621 ccaccgagcg ccccgcgcac cgcgatgacg acgatgatcg cgaaggcggc gacgaacagc
    13681 agcggaaaga gcagagacac cgcagtggtg atcgtcagcc cctgcgcagc gctcgatgcc
    13741 accatgctca cgatcacccc ggcgaagccg aggaagagga agccgatcac gacgaacacg
    13801 aaaaccagca ccacgacccg cgcgacgccg aactggctcg aataggcggg gtcacgcaga
    13861 gcctgctgtc ggaaggtgcg cacctgctcc gaggtgactg gtgccgtgag cgcggtgtag
    13921 tcgaccgatc gagccatgcg cccatggtat cgacgggtgg gaaaagactc atcgcccagc
    13981 ggtgcggcaa tgcctgcgac tcgactgggc gatgagttgt actgcctcca gactgtcacc
    14041 ggctcaacgc ccgcgcaaga ggtctttcgc atccgcgtag actggaacgc cggatttcga
    14101 ggggtgactg ggatgccgtc aggcgagttg gacacggagc gcgcatacgt cgccaccctg
    14161 tacgcgcggc tcgacgcgct gcgcgaggaa gcgcagcaac agctcgaggt cgtgcgccgc
    14221 tccaaccccg ggggcacgca tcagaatcgg tcagagcggg atgccttcgc gcgcatctac
    14281 gaggaccggg tgctgcagct gcgccagatc gacgaacgtc tcgcgttcgg ccgactggag
    14341 atgaagtcgg acggtgccga gccggtgctc cactacatcg gtcggatcgg actgcgcgac
    14401 gaggagcaga agccgctgct gctcgactgg cgggttccgc aggcgcgtgc cttctaccag
    14461 gcgactgccg caacaccgct cggcgcccgg gctcgcagac acctgcagag ccagggtcgc
    14521 gagatcgtgc gaatcgacga cgagatcttc gatcccgacc tgctcgacgg agatgtctcg
    14581 ggcctccagg gcgaggccgc cctcatggcg accctgacgg cgcagcgcac cggacggatg
    14641 tcggacattg tcgcgaccat ccaggccgaa caggatgcga tcatccgctc cgagctgggt
    14701 ggcacgctcg tggtgcaggg cggtccgggc acgggcaaga cggcggtggc gctgcatcgg
    14761 gcggcgtacc tgctgtattc ccatcgggac cggctcgcgg catccggtgt tctcatcgtg
    14821 ggtccgtcgc gatcgttcct gcagtacatc gaggcggtgc tgccgtcgct gggcgagacc
    14881 ggggtggtgc tcgcgagcgt gggccagttg ttcccgggaa tggatgtctc ggcggaggac
    14941 gcgcccgagg ttgcggccct caaggggtca ctcgagatgg cgcaactgct cagtcgtgcg
    15001 gtgcgctcgc ggcaggtggt gcccaccgag ccgcaggtgc tcgacatcaa cggtgagctg
    15061 ctcaccgtgc cgccgcagct catccatgcg gcgatgcagc gggcgtggga gacgaagaag
    15121 ccgcacaatc tcgcccgcgt gacgttcaac aaggccgcga tctccgctct gtctcgcctg
    15181 ctcgccgacc agctgcggtc gcacggcaac accatcgagg acagcgacca ggcctggctg
    15241 cgggaagacc tacgcacgtc gcacgacgtc aaggtcgccc tcaacaccgc gtggctgccc
    15301 ctcacgccgc agaagctcct gcaggacctc tacgccaggc cgcagtggct ggcctcgctc
    15361 accccgcggt ggacggcgga gcagcgcgag ctgctcgcgc gggatcgctc ggccgagttc
    15421 accgtgagcg acgtggccct gctcgacgag gcagccgagc tgctcgggga gtccgacgac
    15481 ctcgccgacg cgcacaagag tgagcgcaag cagcagcgca agcgcgacat cgagaacgcc
    15541 gagcaggcga tccgcaacat gggtgtcgaa ggcctcgtgg atgccgcgac cctcgccgag
    15601 ggcttcgagg ccgcggtgga gcggggcacg acggccgaga tcgcggccgc cgatcgcgag
    15661 tgggcctacg ggcacgtcgt ggtcgacgag gcgcaggaac tctccccgat gcagtggcgc
    15721 ctgctcatgc ggcgaaaccc gctcaagtcg ttcaccatcg tgggcgatgt cgctcaggcg
    15781 agcgcggccg ccggcaa
//
LOCUS       DAOSOU010000019        15579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3801_length_15579_cov_8.247939, whole genome shotgun sequence.
ACCESSION   DAOSOU010000019 DAOSOU010000000
VERSION     DAOSOU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 15579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15579
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3801_length_15579_cov_8.247939"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            540..1088
                     /locus_tag="PLW15_01950"
     CDS             540..1088
                     /locus_tag="PLW15_01950"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOT33195.1"
                     /translation="MNLIFRTIIRMFRSKRAPKLTMSDVGRMEFRVVPTDIDVLGHMN
                     NGVYFSIMDLGRIDLMIRSGAWAKLRAKGYYPVMANETISFRKSLNLWTKFTLETKMA
                     GYDEKAVYCEQRFVVDGEIYAVAMTRARFLKSSGGVVSTAELAEVVGVDATSHTPDAW
                     VAEWAAHVALPSTREPAPSVWD"
     gene            complement(1089..1526)
                     /locus_tag="PLW15_01955"
     CDS             complement(1089..1526)
                     /locus_tag="PLW15_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33196.1"
                     /translation="MAVQPTGRVVRDRRGLELLLERRIPADAATVRNWLTKPHHQRKW
                     LGAAVPVTDGDSPMRIVLDDGAPVVISLAEVDGVTMLFVGERVSDWRAAGAAGPRWEY
                     ALDRLRAAVLGETAPDAAGYLPSQRPYYERLAMDGDPVSWPPS"
     gene            complement(1540..>2187)
                     /locus_tag="PLW15_01960"
     CDS             complement(1540..>2187)
                     /locus_tag="PLW15_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT33197.1"
                     /translation="QVKVNLLLSRLPRLLDPAVDPAAAFGGTFHINETWTQLSTAYDT
                     AAAGAVPEPLPCEIYCHSLTDPSILSPELQASGAQTLTVFGLHTPDRLVSEQNNDERR
                     GELQAAVLASLNSVLAEPVEDLLLTDAAGRPTIETKTTLDLEHALHMPGGNIFHGPLS
                     WPFVEDDAALATPAERWGVATRHPRILLCGSGAQRGGAVSAIGGHNAAMAVLEAS"
     assembly_gap    2188..2287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2288..3164)
                     /locus_tag="PLW15_01965"
     CDS             complement(<2288..3164)
                     /locus_tag="PLW15_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT33198.1"
                     /translation="MSTPRDDRRDVVIVGGGHNGLAAAAYLALAGKRVTVLERLDGLG
                     GAAVSATAFEGVDARLSRYSYLVSLLPERVIRDLGLDITLRRRRYSSYTPEPGSTRGL
                     LIDNADAAATATALGPDAAAWESFYSGTARLAQALFPTVTEPLLTRTEARAAVGDDGL
                     WRDLIERPVGQTIADTFGGDLVRGVVLTDALIGTFAPNIDDSLAANRCFLYHVIGGGT
                     GDWDVPVGGMGAVTGELARAARLAGAELLTGAEVTAITPDGEVSYRSGDAERTLAATW
                     VLANVAPYELERLLGA"
     gene            complement(3145..3633)
                     /locus_tag="PLW15_01970"
     CDS             complement(3145..3633)
                     /locus_tag="PLW15_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOT33199.1"
                     /translation="MTTTTAEERELWRTFFVMRRQLELTLERRLQADSGISSADFEIL
                     AALSEESSAHLRAGRIADIIGWEKSRVSHQLGRMEQRGLIERVECGDDARGVWVVLTA
                     EGRRAVATAMRDHTDAIREYFFDVLDDEHKTALLNASRRVLDVINPPICVDEADRVDA
                     AG"
     gene            3763..7215
                     /locus_tag="PLW15_01975"
     CDS             3763..7215
                     /locus_tag="PLW15_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HOT33200.1"
                     /translation="MLPESDLADEAVATVRRWLTESADIPASPSAARLAGLLKDPKGL
                     DFTLGFVDRVVRPEDLRVAGRNLERLSHSVPSFLPWYLRFAIHAGGGFALLVPWPIVP
                     IARWVLRRMVGHLVIDATPERLDKTLARLRGRGIRLNLNLLGEAVLGQDEANARLDGT
                     RDLLARADVDYVSIKVSSVAAQLSMWAFDDMVEAVVARLTPLYELAADSETTKFINLD
                     MEEYRDLDLTIAVFTKLLDQPRLKNLEAGIVLQAYLPDALGALQRLTAWATERRQSGG
                     AGIKVRVVKGANLAMEHVDAAIHGWPVATYSTKLESDTNYKRVLNWALTPERTDAVRI
                     GVAGHNLFDVAWAWLLATERHVDNRVEFEMLQGMATAQADVVKRDVGGLLLYTPVVHP
                     REFDSAISYLVRRLEENASSENFMSGLFELASNGAVFAREEGRFRASLAALDDRVPGP
                     NRTQNRSKTLEVAPNVDPVDQRFRNEPDTDPSLSINRAWAARVLDRSDQSRLGAGTIA
                     ASRVTEAEALDRLIVAAVRAGEGWGDRSAADRARVLHDAGDVLAAFRGRLIEVMANET
                     GKTIAEGDVEVSEAIDFAHYYAERALELDHLADVTYVPPRLTVVTPPWNFPVAIPAGS
                     VLAALAAGSAVVIKPAPQSRRSGAVMVEALWEAGVPRDVLTLVDLEEGDLGRQLVSHP
                     SVDRVILTGGWETAALFRSWRADLPLLAETSGKNAIIVTPSADLDLAVADVVRSAFGH
                     AGQKCSAASLVILVGSVARSERFSRQLEDAVRTLRVGYPQQASAQMGPLIEPPSGKLL
                     GALTVLGDGEQWLVAPRSLDESGRLWSPGVRTGVAAGSEFHLTEYFGPVLGVMTAATL
                     DDAIALQNAVAYGLTAGIHSLEPREVAHWLDRVEAGNLYVNRGITGAIVRRQPFGGWK
                     RSTVGTGAKAGGPNYVLTLGSWRSIEREPSGNILVDGLSRRVASVVKRGQSGISFEEF
                     DRVRRGALSDEAAWASEFGLSRDVSALEVERNVFRYRPAAVTIRLSEGEPLGHLVRLI
                     AAGSLAGAELTISSAQPLPTALVESFSEPIPTANVRAIVIETDAAWLARAPQLTGRVR
                     LVGGDPLALATAVGGNPDVAIYSAPVTTEGRVELLPFLREQSVSITAHRFGNPDRAMA
                     GLTV"
     gene            7312..8130
                     /locus_tag="PLW15_01980"
     CDS             7312..8130
                     /locus_tag="PLW15_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT33201.1"
                     /translation="MNIPTLALNDGTSIPQLGLGTYGLWNDEGSEVIARAIGLGYRHV
                     DTAARYENEVAVGEGLRRSGVPRGELFVTTKLDGEWQGEDRAVAGLAACLERLGLDYV
                     DLLLIHWPVPKRDEYVSTWRTFERLKSEGLTRSIGVSNFKPAHLERLAAETATVPAVN
                     QIQLTPVIQRHEQRAYDAAHGIVTESWSPLRGVLEREQVVRVAEITGRTPAQVVLRWH
                     LQNGLVAIPKSSNVDRLADNLVALDFTLDDESMAILDTLDEGPHAGVDSDTDGH"
     gene            complement(8127..8483)
                     /locus_tag="PLW15_01985"
     CDS             complement(8127..8483)
                     /locus_tag="PLW15_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT33202.1"
                     /translation="MQFAVDPGSATTLYEQLHRQIIEQIESGELIVGTKLPTVRALAT
                     ELEVAPYTVARVYRALEADGFVETLGRNGTLVKAREGSSVELLQRDATAYAARARELG
                     LPPADALAYIRAALGL"
     gene            complement(8483..8920)
                     /locus_tag="PLW15_01990"
     CDS             complement(8483..8920)
                     /locus_tag="PLW15_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT33203.1"
                     /translation="MDSLDYGIIDLLRLNSRAGYGDIGEVIGLSASAVKRRVDRLVSD
                     GVIRSFTIQVDPAVDGLGTEAYVELFCRGTVAPEELKRILSGVPEVVDAGTVTGSADA
                     IVHMRSRDIPSLEIALEKVRIAPNVDHTRSAIVLSRLIHRTHD"
     gene            9024..9860
                     /locus_tag="PLW15_01995"
     CDS             9024..9860
                     /locus_tag="PLW15_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOT33204.1"
                     /translation="MTMTDAATATPARTARKRTVLMCRPEYFTVSYRINPWMHPEDPT
                     DTSLAVRQWDVLYRTYLDLGFEVHLIDPIAGLPDMVYAANGGFVIDNIAYGASFTYPE
                     RQPEGPAYMDWFREAGFDVRVPEEINEGEGDFLLVNGMILAGTGFRTASNSHEEIARI
                     YGREVVSLNLINPSFYHLDTAVAVLDDTNIAYLPSAFDEASLATLRRLFPDAVIVTEE
                     DAAVLGLNSFSDGYNIVIASRAKDFERQLRERGYNPIGVDLSELLLGGGGVKCCTLEL
                     RR"
     gene            9857..11089
                     /gene="rocD"
                     /locus_tag="PLW15_02000"
     CDS             9857..11089
                     /gene="rocD"
                     /locus_tag="PLW15_02000"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOT33205.1"
                     /translation="MTGAATDLDKLDHRALEDEHVAHNYHPLPIVVGSAAGAWVVDVE
                     GRRYLDCLAAYSAVNFGHGHPELIAAAKAQLDRVTLTSRAFHNDQLGPFATALAALAR
                     KDMVLPMNTGAEAVESGIKVARAWGYRVKGVAPDRATIIVADGNFHGRTTTIISFSND
                     EQARADFGPFTPGFVSVPYGDAAAIEAAIDDNTVAVLIEPIQGEAGIIVPPDGYLAAV
                     RDITSRNNVLFIADEIQSGLGRTGSTFACDLAGVVPDLYLLGKALGGGIVPVSAVVGN
                     RDILGVLRPGEHGSTFGGNPLAAAVGHAVVDLLATGEMQERAGVLGEHLQARLRTLVG
                     RGVVAVRGAGLWVGIDIDPAIGTGRRVCELLMERGVLAKDTHGSTIRLAPPIVVTAEE
                     LDWAVDQLEAVLEELRTG"
     gene            11107..11865
                     /locus_tag="PLW15_02005"
     CDS             11107..11865
                     /locus_tag="PLW15_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT33206.1"
                     /translation="MTEQQLVERLRAAGCVFAEDEARVLLESASSAAELEAMTARRVE
                     GEPLEYVVGWAEFCGLRIGVDATVFIPRPRTEFLVAVAVELTPPSAVVVDLCCGSGAL
                     GLALAASVPDIELLASDIDDAAVACARRNGVAAVQGDLFDALPDRLRGRVDVLLANTP
                     YVPTDELDLMPREARLYEAAVTLDGGSDGLDLQRRVAGGAAHWLAPGGSVLVEASERQ
                     ASTAAAVFEAAGLSARVHYDDDRDATVIVGTRPQ"
     gene            11875..12900
                     /locus_tag="PLW15_02010"
     CDS             11875..12900
                     /locus_tag="PLW15_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT33207.1"
                     /translation="MTLTSLPDADHEPLRGGPTLRWGVLAPGRIAASFVGSLNKSTDQ
                     KVVAVASRSQERAQQFASEHGIDTAYGSYDQLLADDSVDIVYVAAPHNEHARLAHAAI
                     AAGKHVLIEKPIATTVAEARGIADAAAHAGVFAMEAMWSRYLPQATIVAQLLADGALG
                     EPRFVEADFGFLARFDPASRLYNPELAGGALLDLGVYSAWFAHFVLGAPRSVHAVGSL
                     AVTGVDQQSTVTLTYDSDALAVVSSSIIVETPVAARVSGTEALLQFPASFPGPSAFRL
                     AVHGGETLEWRDTSGLNWNEGLCYQAVAAARYVSDGLKQSPLHGLSDSIAVLSVLEQA
                     RAQLGAL"
     gene            12897..14084
                     /locus_tag="PLW15_02015"
     CDS             12897..14084
                     /locus_tag="PLW15_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT33208.1"
                     /translation="MNPLDANDPLARYRERFLPTPGLISYLDGNSLGRPLAASVERVE
                     TFLREEWAGKLIRGWDDGWMSQPLTVGDELGRVALGAAPGQVAIGDSTTVLLYKTIRA
                     ALDARPDRTEIVIDRENFPTDRYLVEGIASDRGLTVRWVDTDPAAGVTPELVAAAVGP
                     DTAVTLFSSVAYRSGFLAEVEAITALVHDAGALTIWDLSHSVGSVPTPLDDWGVDLAV
                     GCGYKYLNGGPGAPAFLYVRRDLQGSLRQPIQGWLGSRTPFEMGQGYEPAEGIRGWLS
                     GTPPMVGLQAMRDMIALIDEVGLDAVRTKSVALTEYAITLVDEHLPTATLASPRDAAR
                     RGGHITVDHPRFAELLPGLWASGVIPDFRRPDGLRLGPSPLSTSYAEVEAGVLAIRDA
                     LGE"
     gene            14133..15227
                     /locus_tag="PLW15_02020"
     CDS             14133..15227
                     /locus_tag="PLW15_02020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT33209.1"
                     /translation="MTVLTPELLERIRSRAAGYDRDNTFFTEDLAELREAGYLAPRSL
                     LETARDQRLLAAYAPATALGINMHHVWVGVARVLHDRGDASLDWVLADASAGELFAFG
                     NSEQGNDLVLWDSTTLAEPVDDGYRFTGTKIFTSLAPAWTRLGIFGRNGEHLVHGFIT
                     RETPGWRTIEDWDTLGMRATQSHTTALEGALVPTDRVARILPVGPNADPFIFGIFSNF
                     LLLIGSVYAGIADRALELAVEAVKRRTSQKTGTTYDQDPDFRWRVADAALALDALAPQ
                     LETLAADVDGLVNHGANWFRYLTGAKHRATETARYVVDQAMRSAGGGGYRTASELSRL
                     QRDVLAGIYHPSDTESVHNTVATNLLGPLA"
     gene            complement(15224..>15579)
                     /locus_tag="PLW15_02025"
     CDS             complement(15224..>15579)
                     /locus_tag="PLW15_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33210.1"
                     /translation="RRTMLAEAGRGWLVELADTVDDPEKVADRMTPQQRMALGALISE
                     LRAIASLVPGSVAVSATTRGSEAVATMTATFTQDGARLRTAAYLAVLRTFFRRATLVI
                     GPTTVTVTLEFVPAN"
BASE COUNT         2326 a         5388 c         5346 g         2419 t
ORIGIN      
        1 agtgcgctcg cgccaagctg gagcccgcat gtctggctgc tcctccccag tcgagttttt
       61 gacgacactg cacgggcctc agctcagcag cacgggagat cacctactga gcgatggtgg
      121 ggcgatgcaa ccgcacgaca tcgttcgaag gctaggcggg gtggcgtggc ggcgggagct
      181 catcgaggcg ggtagctggg acgacatcat cgacatgtcg gtgtctttcg ggcggctccg
      241 aagggttcgt cgcggcctct acgtgctacc cgacatctcg gaggccgccg tcattgcgct
      301 ccgcgtcgga gggcgactgg cctgcatcag ttccctcgca catcacggta tgcgggcggc
      361 agaggccggc gagccagtgc acatcgaact tcctcgaacc gcgtcgagac tccgcctacc
      421 cacaggaaca ccggtggtcc tgcactggac gcgccgaagg catcccggaa cacggctgac
      481 ggtgagcgcg gctgtggcca gatcgcaggc cgcggcctgc tgtgggttag cctgacgcgg
      541 tgaacctgat cttccgcacc atcatccgaa tgttccgcag caagcgggcg ccgaagctca
      601 ccatgagcga tgtcggtcgc atggagttcc gcgtcgtgcc gacggacatc gacgtgctcg
      661 ggcacatgaa caacggcgtc tacttctcga tcatggatct cggccgcatc gacctgatga
      721 tccgctcggg ggcgtgggcc aagcttcgcg ccaagggcta ctacccggtg atggccaacg
      781 agaccatcag ctttcgcaag tcgctcaacc tctggaccaa gttcacgctc gaaaccaaga
      841 tggccggcta cgacgagaag gccgtgtact gcgagcagcg cttcgtcgtc gacggggaga
      901 tctacgcggt cgccatgacg cgtgcccgct tcctgaagtc atcgggtggg gtggtgagca
      961 ctgccgaact cgccgaggtc gtgggggtgg atgccacgag ccacacgccc gacgcctggg
     1021 tcgccgaatg ggccgcgcat gtcgccctcc cgagcacgcg ggagccggcc ccgagcgtct
     1081 gggactagtc agctcggcgg ccagctgacc gggtcgccgt ccatggcgag ccgctcgtag
     1141 tagggccgtt gggagggcag gtagcccgcg gcatccgggg ccgtctcgcc cagcacggcg
     1201 gcgcgcaggc gatcgagcgc atactcccag cgcggcccgg cagcgcccgc tgctcgccaa
     1261 tcgctcacgc gctcgccgac gaagagcatc gtcacgccgt cgacctccgc gagcgagatc
     1321 acgaccgggg cgccgtcgtc gagcacgatg cgcatcggtg agtccccgtc ggtgaccggc
     1381 acagcggcgc cgagccactt gcgctggtgg tggggtttcg tcagccagtt gcgcacggtc
     1441 gcggcatccg cgggaatgcg tcgctcgagc agaagctcga gtccgcgccg atcacgcacc
     1501 acacggcccg tcggttgcac tgccatgtcc tgagactagt caggatgcct cgagcaccgc
     1561 catcgccgca ttgtgtccgc cgatcgcgct caccgcaccc ccgcgctgcg cgcccgagcc
     1621 gcacagcagg atgcgcggat gccgggtggc gaccccccag cgctcggcgg gcgtggcgag
     1681 agcggcgtca tcctcgacga acggccacga gagcggaccg tggaagatgt tgccgccggg
     1741 catgtgcagg gcgtgctcga gatcgagcgt cgtcttcgtc tcgatcgtgg ggcggccggc
     1801 ggcatccgtg agaagcagat cctcgaccgg ctcggccagc accgagttga gcgaggcgag
     1861 cacggcggcc tgaagctccc cccggcgctc atcgttgttc tgctcggaca cgagccggtc
     1921 gggggtgtgc agcccgaaca cggtcaaggt ttgggcgccc gacgcctgca gctcgggcga
     1981 caggatgctc ggatcggtca acgagtggca gtagatctcg cacggcagcg gctcgggcac
     2041 ggctcccgcg gcggcagtgt cgtaggcggt ggagagctgg gtccacgtct cgttgatgtg
     2101 gaaggtgccg ccgaatgccg cggccggatc gaccgcgggg tcgaggagac gcggcaggcg
     2161 cgacagcagc aggttgacct tgacctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnccg cgcccagcag ccgctccagc tcgtacggcg ccacgttcgc gagcacccac
     2341 gtcgccgcga gcgtgcgctc ggcgtccccg ctgcgatagc tgacctcgcc gtccggtgtg
     2401 atcgcggtca cctcggcgcc cgtcagcagc tcggctcccg ccaggcgcgc cgcacgtgcc
     2461 agctcgcccg tgaccgcacc catgccgccc accggaacgt cccagtcccc ggtgccgccg
     2521 ccgatcacgt ggtacaggaa gcagcggttg gcggcgagcg agtcgtcgat gttgggcgcg
     2581 aaggttccga tcagggcgtc agtcagcacg actccccgca cgaggtcccc gccgaaggtg
     2641 tcggcgatcg tctgaccgac cggacgctcg atcaggtccc gccacaaccc gtcgtcgccg
     2701 acggccgcgc gggcctcggt gcgggtgaga agcggttccg tgacggtggg gaagagcgcc
     2761 tgcgcgaggc gcgctgtgcc cgagtagaac gactcccagg cggcggcgtc ggggccgagc
     2821 gcggtggcgg ttgcggcggc atccgcattg tcaatcaaca ggccgcgggt gctgcccggc
     2881 tcgggggtgt agctggagta ccggcggcgc cgcagcgtga tgtcgagccc caggtcgcgg
     2941 atgacgcgct ccggcagcaa cgacaccagg tacgagtagc gcgacagcct ggcgtccacg
     3001 ccctcgaacg cggtcgcgga gacggcggcc ccgccgagcc catcgaggcg ctccagcacg
     3061 gtcacgcgct tgcccgcgag agcgaggtag gcggcggcgg cgagcccgtt gtggccgccg
     3121 cccacgatca cgacgtcgcg gcggtcatcc cgcggcgtcg acacggtccg cctcgtccac
     3181 gcagatcggc gggttgatca cgtcgagcac tcgccgcgag gcgttcagca gcgcggtctt
     3241 gtgctcgtcg tcgagcacgt cgaagaagta ctcgcggatc gcgtcggtgt gatcccgcat
     3301 ggccgtcgcg acggcgcggc gtccctcggc ggtgagcacg acccacacgc cgcgggcatc
     3361 gtcgccgcac tcgacccgct cgatgaggcc gcgctgctcc atgcggccga gctggtgcga
     3421 gacccgactc ttctcccagc cgatgatgtc ggcgatacga ccggcccgca gatgcgcgct
     3481 ggactcctcg gagagtgcgg cgagaatctc gaagtccgcg gaggagatgc cggagtcggc
     3541 ctgcagtcgc cgctcaaggg tgagttcgag ctgacgacgc atcacgaaga aggttcgcca
     3601 gagctcgcgc tcctcggcgg tcgtcgttgt catgccgcaa ttgtacggat cgtgccctcg
     3661 tggcagctag ggcgctacaa agcgtcgtct gcgccatccc tcacgcaaga gttgtgcgtc
     3721 agtatgcgct cttcgctggg atgccgcggt taagctcgat gcatgctgcc cgagtccgat
     3781 ctcgccgacg aggccgtcgc caccgtccgc cgctggctca ccgagagcgc cgacattccg
     3841 gcgtcgccca gtgcggcccg gctggccggc ctgctcaaag acccgaaggg tctcgacttc
     3901 accctcggat tcgtcgaccg ggtggtgcgg cccgaggatc tgcgggtcgc cggccgcaac
     3961 cttgagcgtc tcagccactc ggtgccgtcg ttcctgccgt ggtacctgcg cttcgccatc
     4021 cacgccggtg gcgggttcgc ccttctcgtt ccgtggccga tcgtgccgat cgcgcggtgg
     4081 gtgctgcggc gcatggtcgg ccacttggtc atcgacgcga cgccggaacg cctcgacaag
     4141 actctggcgc gattgcgggg tcgtggcatc cggctcaacc tgaacctgct cggcgaggcg
     4201 gtgctcggcc aggacgaggc gaacgcccgg ctcgacggca cccgcgacct gctcgcccgg
     4261 gccgacgtcg actacgtgtc gatcaaggtg tcgtcggtgg cggcccagct gtcgatgtgg
     4321 gccttcgacg acatggtcga ggcggtcgtc gctcggctga ccccgctcta cgagctggcc
     4381 gccgacagcg agaccaccaa gttcatcaac ctcgacatgg aggagtaccg cgatctcgac
     4441 ctgacgatcg cggtgttcac gaagctgctc gaccagccgc ggttgaagaa cctcgaggcc
     4501 ggcatcgtgc tgcaggccta cctgcccgac gccctcggtg ccctccagcg gctgacggcc
     4561 tgggcgaccg agcgtcgcca gtccggcggc gccggcatca aggtgcgcgt ggtcaagggc
     4621 gccaacctcg ccatggagca cgtggatgcc gcgatccacg gctggcccgt cgccacctac
     4681 tcgaccaagc tcgagtcgga caccaactac aagcgggtgc tcaactgggc tttgaccccc
     4741 gagcgcacgg atgcggtgcg catcggcgtc gccggccaca acctgttcga cgtggcgtgg
     4801 gcctggctgc tcgccaccga acgccacgtc gacaaccggg tcgagttcga gatgctccag
     4861 ggcatggcga cggcccaagc ggatgtcgtc aagcgtgacg tgggcggcct gctgctgtac
     4921 acgcccgtcg tgcacccccg tgagttcgac tcggcgatca gttacctcgt gcgccggctc
     4981 gaggagaacg catcgagcga gaacttcatg agcgggctgt tcgagctcgc ctcgaacggc
     5041 gcagtgttcg cgcgcgagga gggacgcttc cgggcgtcgc ttgccgcact cgatgaccgg
     5101 gtgcccggac cgaaccgcac ccagaaccgc tcgaagaccc tcgaggtggc ccccaacgtc
     5161 gaccccgtgg accagcgctt ccgcaacgag cccgacaccg acccgtcgtt gagcatcaac
     5221 cgcgcgtggg ccgcgcgggt gctcgaccgc agcgaccagt cgcgtctcgg cgccggcacc
     5281 atcgcggcgt cccgcgtgac cgaggccgag gctctcgatc gactcatcgt cgcggcggtg
     5341 cgcgccgggg agggctgggg cgatcgctcg gcggccgatc gcgcgcgtgt gctgcacgat
     5401 gcgggtgatg tgctcgcggc gttccgcggc cggctcatcg aggtcatggc caacgagacg
     5461 ggcaagacca tcgcggaggg tgacgtcgag gtcagcgagg ccatcgactt cgcccactac
     5521 tacgccgagc gcgccctcga gctcgatcac ctcgccgacg tcacctacgt gccgccgcgc
     5581 ctgaccgtgg tcaccccgcc ctggaacttc ccggtcgcca tccccgccgg ctcggtcctg
     5641 gcggcgctcg ccgcgggcag cgccgtcgtc atcaagcccg ctccgcagtc ccgccgcagc
     5701 ggcgcggtca tggtggaggc gctgtgggag gcgggggtgc cgcgggatgt cctcaccctc
     5761 gtcgatctcg aggagggcga cctcggccgc cagttggtat cgcacccgag cgtcgatcgc
     5821 gtcatcctga ccggagggtg ggagacggcg gcgctattcc ggtcgtggcg ggccgacctg
     5881 ccgctgctgg cggagacgag cggcaagaac gccatcatcg tgaccccgag cgccgacctc
     5941 gacctcgcgg tcgccgatgt ggtgcgcagc gccttcggtc acgccggcca gaagtgctcc
     6001 gccgcttcgc tcgtgatcct cgtcggatcg gtcgcccgca gcgaacggtt ctcgcgccag
     6061 ctcgaggatg ccgtgcgcac cctgcgggtc ggctatccgc agcaggcgag cgcgcagatg
     6121 gggccgctca tcgaaccacc gtcgggcaag ctgctcggcg cactgaccgt gctcggcgac
     6181 ggcgagcagt ggcttgttgc cccgcgctcg ctcgacgagt cgggtcgact gtggtcgccg
     6241 ggggtgcgca cgggggttgc ggcaggcagt gagttccacc tcacggagta cttcggtccc
     6301 gtgctcggcg tgatgacagc ggcgacgctc gacgacgcga tcgcgctgca gaacgccgtt
     6361 gcctatgggc tcacggcggg catccactcg ctggaaccgc gcgaggtcgc gcactggctc
     6421 gaccgcgtcg aggcaggcaa cctctacgtc aaccgcggaa tcaccggcgc gatcgtgcgt
     6481 cgccagccgt tcggcggctg gaagcgctcg acggtcggca ccggtgcgaa ggccggcggc
     6541 cccaactacg tgctgacgct cggctcctgg cggtccatcg agcgcgagcc gtcggggaac
     6601 attctcgtcg acggcctcag tcgccgagtg gcctcggtgg tcaagcgggg tcagtcgggt
     6661 atctcgttcg aggagttcga ccgggttcgt cgcggtgcgc tcagcgacga ggcggcgtgg
     6721 gcgagcgagt tcggtctcag ccgggatgtc tcggcgctcg aagtcgaacg caacgtgttc
     6781 cgctaccggc ccgctgccgt gaccatccgc ctctccgagg gcgagccgct cggtcacctc
     6841 gtgcgcctga tcgccgcggg ctcgctcgcg ggcgcggagt tgaccatcag ctccgcgcag
     6901 ccgctgccca ctgctctggt cgaatcgttc agcgaaccga ttccgacggc gaacgtgcgc
     6961 gcgatcgtca tcgagacgga tgccgcgtgg ctggcccgag cgccgcagct caccggtcgc
     7021 gtgcgtctcg tcggaggcga cccgctcgcc ctcgcgactg ccgtcggtgg caacccggac
     7081 gtcgcgatct actcggctcc cgtcaccact gaggggcgcg tcgagctgct cccgttcctg
     7141 cgcgagcaga gcgtgtcgat cactgcccac cgcttcggta accccgatcg ggcgatggcg
     7201 ggcctcacgg tctaggcggg tcgagtagcg gcggagccgc gtatcgagac cttcacggtc
     7261 ggtttcgata cgcgaccttc ggtcgctact caaccggcct acgctggtcg catgaacatc
     7321 cccactctcg ccctcaacga cggaacgagc atcccgcaac tgggcctggg cacgtacggc
     7381 ctctggaacg atgaggggtc ggaggtgatc gctcgggcga tcggcctcgg ctaccgtcac
     7441 gtcgacaccg cggcccggta cgagaacgag gtcgcggtcg gggaggggct gcgtcgttcg
     7501 ggggtcccgc gcggcgagct gttcgtgacc acgaagctcg acggcgagtg gcagggcgag
     7561 gaccgcgccg tcgcggggct ggccgcgtgc ctggaacgcc tcgggctcga ctacgtcgac
     7621 ctgcttctca tccactggcc ggtgccgaag cgcgacgagt acgtgtcgac ctggcgcacc
     7681 ttcgagcgac tcaagtccga gggcctcacg cgctcgatcg gggtgtccaa cttcaagccc
     7741 gcgcacctcg agcgcctcgc cgccgagacc gcgacggtac cggcggtcaa ccagattcag
     7801 ctgacgccgg tgatccagcg gcacgagcag cgggcatacg acgccgcgca cggcatcgtc
     7861 accgagtcat ggagccccct gaggggagtt ctcgagcggg agcaggtggt gagggtcgcc
     7921 gagatcaccg gccggactcc cgcccaggtc gtgctgcggt ggcacctgca gaacggactc
     7981 gttgccatcc cgaagtcgtc gaatgtcgac cggctcgccg acaacctcgt cgcactcgac
     8041 ttcaccctcg acgacgagtc gatggcgatc ctcgacaccc ttgatgaggg tccgcacgcg
     8101 ggcgtcgact ccgacacgga cgggcactag agcccgagcg cggcccggat ataggcgagc
     8161 gcgtcggcgg gcggcagccc gagctcgcgt gcgcgtgcgg cgtaggccgt ggcatcccgc
     8221 tgcagaagct cgaccgagct gccctccctc gccttcacga gggtgccgtt gcggccgagg
     8281 gtctcgacga aaccgtcggc ctcgagcgcg cggtagaccc gggcgacggt gtagggcgcg
     8341 acctccagtt cggtcgccag ggcgcgcacg gtcggcagct tcgtgccgac gatcagctcg
     8401 cccgactcga tctgctcgat gatctggcgg tgcagctgct cgtagagcgt ggtcgctgac
     8461 ccggggtcga cggcgaactg catcagtcgt gggtcctgtg gatgagccgt gacaacacga
     8521 tcgcgctgcg ggtgtgatcg acgttgggag cgatccgcac cttctcgagc gcgatctcca
     8581 aggaggggat gtcgcgtgac cgcatgtgca ctatcgcgtc ggcagagccg gtcacggtgc
     8641 cggcatcgac gacctcggga actccggaca ggatgcgctt gagctcctcc ggcgcgacgg
     8701 tgccccggca gaacagttcg acgtaggcct cggtgccgag cccgtcgacg gccgggtcga
     8761 cctgaatggt gaagctgcgg atgacgccat cagacaccaa ccggtccacc cgacgcttga
     8821 cggccgacgc cgacaggccg atgacctcgc cgatgtctcc gtatccggcg cgggaattga
     8881 ggcgcaggag gtcgatgatg ccgtagtcga gactgtccat ccgggcatcc tatgacgaat
     8941 agttgtgctg cgatagcgtc agacgcaggt tcggtgcgct ccaagctctg acattgcgga
     9001 taggtgcgtg aaacactgga cgaatgacga tgaccgacgc cgctacggcc acgcccgccc
     9061 gcactgcccg caagcgcacc gtgctcatgt gccggcccga gtacttcact gtgagctacc
     9121 gcatcaaccc gtggatgcac cccgaagacc ccaccgacac gagccttgcc gtgcgccaat
     9181 gggatgtgct ctaccgcacc tacctcgacc tcgggttcga ggtgcacctc atcgacccga
     9241 tcgcgggcct ccccgacatg gtctacgcgg ccaacggcgg cttcgtgatc gacaacatcg
     9301 cgtacggcgc gagcttcacc taccccgagc gccagcccga gggcccggcc tacatggact
     9361 ggttccgtga ggcgggcttc gacgtgcgcg ttcccgagga gatcaacgag ggcgagggtg
     9421 acttcctgct cgtgaacggg atgatcctcg ctggtacggg cttccgtacc gcaagcaaca
     9481 gccacgagga gatcgcccgc atctacggtc gtgaggtggt gtccctcaat ctgatcaacc
     9541 cgagcttcta ccacctcgat accgccgtcg cggttctgga tgacacgaac atcgcgtacc
     9601 tcccgagtgc cttcgacgag gcatccctcg ccaccctgcg caggctcttc cccgatgccg
     9661 tcatcgtcac cgaggaggac gcggcggtgc tgggcctcaa ctcgttcagc gacggctaca
     9721 acatcgtgat cgcctcgcgg gccaaggact tcgagcggca gttgcgcgag cgcggctaca
     9781 accccatcgg cgtggacctg tccgagcttc tgctcggcgg cggaggtgtg aagtgctgca
     9841 cgttggagct tcgccggtga ccggtgccgc gactgatctc gacaagctcg atcaccgggc
     9901 actggaggac gagcacgtcg cccacaacta ccacccgttg cccatcgtgg tgggctccgc
     9961 ggccggcgcc tgggtcgtcg acgtcgaggg gcggcgctac ctggactgcc tcgcggcgta
    10021 ctccgcggtc aacttcggcc acgggcatcc ggagctcatc gcggcggcca aggcccagct
    10081 cgaccgggtg acactgacca gccgggcgtt ccacaacgac cagctcggcc cgttcgcgac
    10141 cgcgctcgcc gccctcgccc gcaaagacat ggtgctgccc atgaacaccg gtgccgaggc
    10201 cgtggagtcg ggcatcaagg tcgctcgcgc ctggggctac cgcgtcaagg gggtcgcccc
    10261 cgatcgcgcg acgatcatcg tggccgacgg caacttccac ggccgcacga ccaccatcat
    10321 cagcttctcg aacgacgagc aggctcgggc cgacttcggt ccgttcaccc ccggcttcgt
    10381 gagtgtgccc tatggggatg ccgcagccat cgaagcggcg atcgacgaca acaccgtggc
    10441 cgtgctcatc gagccgatcc agggtgaggc gggcatcatc gttccgccgg acgggtatct
    10501 cgctgccgtg cgggacatca cgtcgcgcaa caacgtgctc ttcatcgccg acgagatcca
    10561 gtccggcctg gggcgcaccg gctcgacctt cgcctgcgat ctcgcgggag tcgtgcccga
    10621 tctctacctg ctcggcaagg cgctcggcgg cggaatcgtg ccggtgagcg cggtcgtcgg
    10681 caaccgcgac atcctcgggg tgctacgtcc gggcgagcac ggctcgacct tcggcggcaa
    10741 cccgctcgca gcggccgtcg gtcatgccgt ggtcgacctt ctcgcgaccg gtgagatgca
    10801 ggagcgcgcg ggcgtgctcg gcgagcacct gcaggcgcgg ctgcgcaccc tcgtcggccg
    10861 cggcgtggtc gccgtgcgcg gcgcgggatt gtgggtgggc atcgacatcg accccgccat
    10921 cggaaccggt cggcgggtgt gcgagctgct gatggagcgc ggggtgctcg cgaaggacac
    10981 ccacgggtcg accatccggc tcgccccgcc gatcgttgtc actgctgagg aactcgactg
    11041 ggctgtcgac cagctcgagg cggtgctcga ggagttgcgc accggctgac ggtctagatt
    11101 cgggggatga ccgaacagca gctcgtggag cgcctgaggg cggccggctg cgtgttcgcc
    11161 gaggatgagg cgcgcgtgct gctggagtcc gcgtcatccg ccgccgagct ggaggccatg
    11221 actgcccgtc gcgtcgaggg cgagccgctc gagtacgtcg tcggctgggc cgaattctgc
    11281 gggcttcgca tcggcgtgga tgccacggtc ttcatcccca ggccgcgcac cgagttcctc
    11341 gtggccgtcg ccgtggagct cacgccacct tccgcagtgg tcgtcgacct gtgttgcgga
    11401 tccggcgcgc tcggactcgc gctcgcggca tccgtgccgg acatcgagct gctggccagc
    11461 gacatcgacg acgctgccgt cgcctgcgcc cggcgcaacg gggtcgcggc ggtgcagggt
    11521 gacctgttcg acgcgctgcc cgatcggctg cgcggtcggg tcgacgtgct cctcgccaac
    11581 actccctacg tgccgaccga cgagctcgat ctcatgcccc gcgaggcgcg gctgtacgag
    11641 gcggcggtga cgctcgacgg cgggtcggat ggcctcgacc tgcagcgcag ggtggccggg
    11701 ggtgcggcgc actggctggc gccgggcggc agcgtgctcg tcgaggcgag cgagcggcag
    11761 gcgtcgaccg ccgcagccgt cttcgaggct gcgggcctga gtgcgcgggt gcactacgac
    11821 gacgaccggg atgcgaccgt gatcgtgggc acccggccgc agtagattcg ttccatgacc
    11881 ctcacgagcc tgcccgacgc cgaccacgaa ccactgcgcg gcggtcccac gttgcgctgg
    11941 ggtgtcctcg cgccggggcg catcgcggcg tcgttcgtgg ggtcgctgaa caagtccacc
    12001 gaccagaagg tcgtggccgt ggcatcccga agccaggaac gtgcgcagca gttcgcgagc
    12061 gagcacggca tcgacaccgc ctatggcagt tacgaccagc tgctcgccga cgactcggtc
    12121 gacatcgtgt acgtcgccgc accccacaac gagcacgcgc ggctcgcaca cgctgcgatc
    12181 gctgcgggca agcatgtgct catcgagaag ccgatcgcaa caacggtcgc tgaggctcgt
    12241 ggcatcgccg acgccgccgc gcacgccggc gtcttcgcga tggaggcgat gtggagccgg
    12301 tacctgcctc aggcgaccat cgtcgcccag ctgttggctg acggagccct gggagagccc
    12361 cggttcgtcg aggctgactt cggatttctc gcgcgcttcg atccggcttc ccggctctac
    12421 aatcccgagc tggcgggcgg agcgctgctc gacctcggcg tctactcggc gtggttcgcc
    12481 cacttcgtac tgggagcacc acgatcagtg cacgccgtgg ggtcgctcgc agtgaccggg
    12541 gtcgaccagc agtcgaccgt caccctgacc tacgactcgg atgccctcgc cgtcgtctcg
    12601 agcagcatca tcgtcgagac tcccgtggcg gcgcgggtga gtggtaccga ggcgttgctc
    12661 cagtttccgg cgagcttccc cggacccagt gcgttccggc tcgcggtcca cggcggtgag
    12721 acactcgaat ggcgcgacac gagcggcctc aactggaacg aggggctctg ctaccaggcg
    12781 gtggctgcgg ctcggtacgt cagtgacggg ctgaagcagt cgccgcttca cggtctcagc
    12841 gactcgatcg ccgtgctctc ggtgctcgaa caggcacggg cgcaactcgg ggcgctatga
    12901 acccgctcga cgcgaacgat ccgctggcac gataccgcga gcgcttcctg ccgacacccg
    12961 ggctgatctc ctacctcgac ggcaactccc tcggacggcc cctcgcggcc tcagtcgagc
    13021 gggtcgagac gtttctgcgc gaggagtggg ccggcaagct gatccgcggc tgggacgacg
    13081 gctggatgag ccagcccctc accgtcggtg acgaactcgg tcgggtcgcc ctcggcgcgg
    13141 caccgggcca ggtggcgatc ggggattcca cgacggtgct gctctacaag acgattcggg
    13201 cggcgctgga tgcccgtccc gaccgaaccg agatcgtgat cgatcgcgag aacttcccga
    13261 ccgaccgcta tctcgtcgag ggcatcgcgt cagaccgggg actcaccgtg cgctgggtcg
    13321 acaccgaccc tgcggcgggg gtgacacccg agctcgtggc cgccgccgtc ggccccgaca
    13381 cggcggtgac gctgttctcc agcgtcgcct accgcagcgg cttcctcgcc gaggtcgagg
    13441 cgatcacggc cctcgtgcac gacgcgggag cgctcaccat ctgggatctc tcgcattccg
    13501 tcggttcggt gccgactccg ctcgacgact ggggcgtcga cctcgctgtc ggctgcgggt
    13561 acaagtacct gaacggcggg cccggcgctc cggcgttcct ctacgtgcgg cgcgacctgc
    13621 agggcagcct gcgccagccg atccagggct ggctcggcag ccgcacgccc ttcgagatgg
    13681 ggcagggcta cgagcccgcc gagggcatcc gcggctggct cagcggaaca ccgcccatgg
    13741 tgggtctgca ggccatgcgc gacatgatcg cgctcatcga cgaggtgggc ctcgacgcgg
    13801 tgcgcaccaa gtcggttgcc ctcaccgagt atgcgatcac cctcgtcgac gagcatctgc
    13861 cgaccgcgac gctggccagc ccgcgagacg ccgcacgtcg cggcggtcac atcacggtgg
    13921 accacccgcg gttcgccgaa ctgctgcccg ggctctgggc gagtggcgtc atcccggact
    13981 tccgtcgtcc cgatgggctg cgcctgggtc cgtcgcccct ctcgacgagc tacgccgagg
    14041 tcgaggcggg cgtgctggcg atccgggatg cgctaggcga gtaggacctc gaaggcgccg
    14101 ctcgtgcgct ctgacggacg tagcctgaag gggtgactgt tctgactccc gagttgctcg
    14161 agcgcatccg ctcgcgcgcg gccggctacg accgcgacaa caccttcttc accgaggacc
    14221 tcgcagagct gcgcgaggcg ggctacctcg ccccgcgcag cctgctcgag accgcgcgcg
    14281 accagcgact gctcgcggcc tatgcgcccg cgacggcgct cggcatcaac atgcaccacg
    14341 tgtgggtggg cgttgcgcgc gtgctgcacg atcgcggcga cgcaagcctc gattgggtgc
    14401 tggcggatgc ctcggccggc gaactcttcg ccttcggtaa cagcgagcag ggcaacgatc
    14461 tcgtgctgtg ggactcgacg accctcgccg aaccggtcga tgacggctac cgcttcaccg
    14521 gcaccaagat cttcacctcg ctcgcgccgg cgtggacgcg gctgggcatc ttcggccgca
    14581 acggcgagca cctcgtgcac gggttcatca cccgcgagac ccccggatgg cgcaccatcg
    14641 aggactggga cacgctcggg atgcgggcga cgcagagtca caccaccgcg ctcgagggtg
    14701 cgctcgtgcc gaccgaccgg gtggcgcgca tcctgccggt agggcccaac gccgacccct
    14761 tcatcttcgg catcttctcc aacttcctgc tgctcatcgg ctcggtgtac gcgggcatcg
    14821 cggatcgcgc cctcgaactc gccgtcgagg cggtgaagcg ccgcacgtcg cagaagacgg
    14881 gcaccaccta cgaccaggat cccgacttcc ggtggcgcgt cgccgatgcc gcgctcgcgc
    14941 tcgatgctct cgccccacag ctcgagacct tggccgcgga tgtcgacggg ctcgtgaacc
    15001 acggcgcgaa ctggttccgc tacctcaccg gggcgaagca ccgcgccacc gagactgcgc
    15061 gctatgtcgt cgaccaggcc atgcgcagcg ccggcggtgg cggttaccgc accgcgtccg
    15121 agctgtcccg cctgcagcgc gacgtgctcg cgggcatcta ccacccgagc gataccgagt
    15181 cggtgcataa caccgtcgcc acgaacctgc tcgggccgct cgcctagttc gcgggaacga
    15241 attccagggt gacggtgacg gttgtcggcc cgatcaccag ggttgcccga cggaagaacg
    15301 tacggagcac cgcgaggtag gcggcggtgc gcagccgcgc gccgtcctgc gtgaatgtcg
    15361 ccgtcatcgt cgcgaccgcc tcgctgcccc gggttgtggc actgacggcg acgctgccgg
    15421 gaaccagcga cgcaattgcc cgcagttcgg agatgagggc gcccagagcc atccgctgtt
    15481 ggggcgtcat ccggtcggcg accttctcgg ggtcatcgac cgtgtcggcg agttcgacga
    15541 gccagccccg tcccgcctcg gccagcatgg tgcgtcgca
//
LOCUS       DAOSOU010000020        15471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3836_length_15471_cov_6.866697, whole genome shotgun sequence.
ACCESSION   DAOSOU010000020 DAOSOU010000000
VERSION     DAOSOU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_15471_cov_6.866697"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLW15_02030"
     CDS             <1..592
                     /locus_tag="PLW15_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33211.1"
                     /translation="LALIERFYDPNAGVVRLGGIDIRGLDRTDLRARIGYVEQDAPVL
                     AGTIRENLRLATPDATDEQCIEVLHAVNLTEVLERNELGLDAPVGEDGVMLSGGERQR
                     LAIARTLLAAPPILLLDESTSSLDGLNEQMLREAIDAVAQNRTLIVIAHRLSTVVDSD
                     QIVVLDHGQVVGVGTHSELVKSTPLYKDLAKHQLLV"
     gene            complement(807..1607)
                     /locus_tag="PLW15_02035"
     CDS             complement(807..1607)
                     /locus_tag="PLW15_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT33212.1"
                     /translation="MTVIEIDSLDDPRLADYSHQTDVALKKAQGTEHGLYIAESALVL
                     GRALRAGHRPRSVLALGSSVSDALELTAGHDVPVFHGPPELLEQLTGYLLHRGLIASM
                     HRPALPTVESLLRDARRIVILENVADPTNVGAIFRSVAGIGADAVLVTPRCSDPFYRR
                     AIRVSMGTVLQVPWTRLGDWGSAREVLTAAGFHVAALALEPGAVDLREFAASAPERVA
                     LVLGEEGYGLTREGLDAADSVVQIPMAHGIDSLNVAATAAVAMYALAG"
     gene            complement(1639..3105)
                     /locus_tag="PLW15_02040"
     CDS             complement(1639..3105)
                     /locus_tag="PLW15_02040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOT33213.1"
                     /translation="MISESELLAKVPDRLFIGGEWVESTSGRSIDVHDPATGKVIKTI
                     ADASVADSAAAMDAAADTQASWGATAPRVRGEILRGAFDLLQERADEFALLMTLEMGK
                     PLAEARGEVTYGGEFLRWFGEEAVRITGRYGSNPEGTGRMIVTHLPVGPSFLITPWNF
                     PLAMATRKIAPAIAAGCTSIIKPAALTPLTTLYFAKLLEDAGLPKGVVNVLTTSTSRA
                     ISDPIIADPRLRKLSFTGSTGVGQQLMKQASEGVLRTSMELGGNAPFVVFEDADLDKA
                     VDGAMLAKFRNIGQACTAANRFIVHESVADEFARRVTERVTAFPIGRGTEPGVIIGPL
                     IDDDAVDKADALVQDAVTKGASILTGGKRVEGDGSFYEPTVITGVNPGSEILREEIFG
                     PVLSITTFTDEADAVAKANDTEYGLVSYVYTQDFARGQRLIETLQTGMMGLNVGVVSN
                     AAAPFGGMKQSGVGREGGFEGIHEYLETKYVLTPNPFA"
     gene            complement(3142..3708)
                     /locus_tag="PLW15_02045"
     CDS             complement(3142..3708)
                     /locus_tag="PLW15_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT33214.1"
                     /translation="MSRALFIIDVQNDFTEGGALGVEGGAAVAEGITKLLHGHPDRYD
                     AVIASRDWHDGDNDNGGHFHPAPDYVDSWPEHCVSRTEGAEYHPALEDALIDVHVLKG
                     QGKPAYSIFEGVTDTGETVPELLDRLDVTDIDVVGIATDYCVLASALDARKAGRRVRV
                     LTELVAGVAPVSSAAALETMASAGVILS"
     gene            complement(3708..5168)
                     /gene="dacB"
                     /locus_tag="PLW15_02050"
     CDS             complement(3708..5168)
                     /gene="dacB"
                     /locus_tag="PLW15_02050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOT33215.1"
                     /translation="MTDSNDSVPTQALSRRSLRTPEPKAPGRISAIIRKHPTAWLISA
                     IAVVFLLLGTSAVFAGVAAGGGPTVAAPLPTETADPRPQPSAVPVASRLRTCSVASVM
                     ADPRLADFAGYVVNTATGETLFDHSGTLPQRTGSVLKVLTAAAALNILGPGGQLTTQV
                     IDGSSPGVIVLKGGGDPTLATTSNTVYDGAPLIADLADAAMAKYEQVHPGVPITEIVL
                     DSTMWDPADNWDSSWLRKEQDDGYQAQVTALMVDGGRADPSANVSYRTTDPVGDAGRA
                     FADAAGLFGVTFSSGAAVGSTVLAEVKSQPVSSLIDKMLLSSDNVLAEMLARVVSKAM
                     GFDGSSTSLAQAIPGALVAYGLDTTKVTVRDGSGLSDLNAVSPQFVTALLTKVNAGEK
                     DLGVLYSSLPVAGVSGSLASRFNGPNAIAAGSVMAKTGWLDTEYSLAGIIKAADGSTL
                     AFAFYSIREGITEDAKEAQDTLATAMYSCGDNLSNN"
     gene            complement(5214..6059)
                     /locus_tag="PLW15_02055"
     CDS             complement(5214..6059)
                     /locus_tag="PLW15_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33216.1"
                     /translation="MKSYAFLATVFSTAVIVALPFVGGGGPGLTGLAVADPSVAAAPM
                     LASVIAAEQSQPADQAALRYSGSDQVIEARIVAESKSLTSDWEHLAQKPVPKPLPPVD
                     AAGAASGTNERRAALEAQGLSCPGFSAGGTSGAPGAVSALGVPGTTSDDLASFAYQYN
                     AIRAENCLEPVSYFVYDSCMEARLFWMAESPSPDPMDAWGHMGSVRIDGVPSVGCDGN
                     LAGGSGNSGATVAIKWWQSSSHQASLYRPGAGTGGVCIAFAMTHGGIDEPYSFTRAAA
                     RWTYC"
     gene            complement(6236..6311)
                     /locus_tag="PLW15_02060"
     tRNA            complement(6236..6311)
                     /locus_tag="PLW15_02060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6276..6278),aa:Thr,seq:cgt)
     gene            complement(6384..7013)
                     /locus_tag="PLW15_02065"
     CDS             complement(6384..7013)
                     /locus_tag="PLW15_02065"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT33217.1"
                     /translation="MTIATDAAEPGLRERKRRATRRAIQLAALQLVAERGLDNVTIDE
                     VSRIADVSPRTFFNYFASKEEAILGDPPTLAETDTVERFVTNADNSGVFDGLVAVLIE
                     VGDSSLEDAEMMQLRHALVKEYPQLFALRMAKMRDFEERVASVIVRRLRHDQPDAEAS
                     VLERRARLMTHVSLGVIRHAWASWATSEPRTELGVQLRRSFAELKTLFV"
     gene            complement(7056..8600)
                     /locus_tag="PLW15_02070"
     CDS             complement(7056..8600)
                     /locus_tag="PLW15_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33218.1"
                     /translation="MNTASFRATALIATGSALLLLLSGCFNFGVLQPDNTTKPTGEDV
                     PAALVPYYTQVLDWADCDNGMECATAKAPMDWANPSPETDIELAVVRHPATGTKQGSL
                     FTNPGGPGASGFDFVYDSLDFAASADLQAAFDVVGWDPRGVGRSSAVKCYEAPELDAF
                     IFGLPQAPRGTQAWIDEVTQSSIDFGKACLENTGELLQFIDTQSTVHDLDMLRAVVGD
                     PKLNYLGYSYGSDIGSYYVENFPDKVGRLVVDGATDSSISVFEVGLVQTKGFQRALEN
                     YLKACPDLFDDCPFTGDLTTDLATIRGLYDRYDASPLAAPDGRMMDAGVLDIAMSMAL
                     YSQDSWPFLNDLFSEAQDGVTDTAFFLADYYYSRDTDGTYADNSLEAFLAIYCVDYPV
                     ETDPTVLAEQEVLLEQASPTTYRPSPPIGDTTCINWPYQYQGPPIAALTGKGAPPVLV
                     VSTTGDPATPYEWGVALADQLESAVLITFNGEGHTAYGQGNECITSTVDQYLLKGVVP
                     TADPNC"
     gene            complement(8633..10162)
                     /locus_tag="PLW15_02075"
     CDS             complement(8633..10162)
                     /locus_tag="PLW15_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33219.1"
                     /translation="MRSLRLGLVALALAATLPLSGCVSWFLPPAASTSTPTGEDVAAE
                     LKPYYGQVLTWSSCGGGMQCATAKAPMDWSDPSAGDIELALVRQPATSGNRVGSLLVN
                     PGGPGGSGYDFVKDSVDYATDERLQASFDVVGFDPRGVNRSTPVTCYTDPAELDAYIY
                     DIVPGEPGSDEWLAELGANSKAFGERCLELTGPLLGLVDTPSAARDLDMLRAALGDTT
                     LNYLGYSYGTLLGQVYADLFPDKTGRLVLDGAVDPAATDFEGTATQAKGFESALKAFL
                     ADCAGASDCPFSGSVDDSLTEIRALLDNLDASPLRNSDGRQLGSSAMFTAIILPLYNK
                     DNWQYLRQLFTDVFAGDATYAFQLADNYNGRNEDGTYRDNQTEAFISINCLDAHGDGD
                     VATMRAEAAELKQLAPVFGPQMSWGGTGCPNWPVPAKRDRAPIVADGSSDILVIGTTN
                     DPATPYSWAVTVANTLSKGHLVTYEGEGHTAYNKSNACVNDTVDDFLINGTVPAADPK
                     C"
     gene            complement(10159..11313)
                     /locus_tag="PLW15_02080"
     CDS             complement(10159..11313)
                     /locus_tag="PLW15_02080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOT33220.1"
                     /translation="MAIWDELTGQADAIAVVEAAAAASRVGGSDDSSMTHSWLITGPP
                     GSGRSNLAYAFATALLGGDDEATRRQVQARTHPDLGVLATERVIISIEEVRQLVATSQ
                     FSPSVGRYRVMVIEDADRMTERTSNVLLKALEEPPPRTVWILCAPSEADLIPTIRSRV
                     RTVRLRVPSVDEVASLLERRDGVDAATATRAAREAQSHIGMAHRLATNVQARQRRERT
                     LDVALGIRSVSDATNAATVLLDLAKADAEAITEERDSEERDAALRSLGIDPGGTIPPA
                     LRSQLKALEEDQKRRATRSLRDGIDRIMVDLLSLYRDILLIQLGVPGDPINLSILGRV
                     EAAAALSDAAGTLATMDAIAIARRRIDSNVAPALALEAMLVTAVRKAAAQ"
     gene            complement(11520..12140)
                     /gene="tmk"
                     /locus_tag="PLW15_02085"
     CDS             complement(11520..12140)
                     /gene="tmk"
                     /locus_tag="PLW15_02085"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT33221.1"
                     /translation="MRGLFITFEGGDGSGKSTQSELLVQWLEAQGRTVVVSREPGGTD
                     LGLELREIVLHRRGEIAPRAEALIYAADRAHNIATKVRPALERGDIVVQDRYLDSSVA
                     YQGAGRVLGADEVRDVSLWATESLLPDLTILLDLHEGQDRLADRTKYDRLEAAGDDFH
                     ARVREAYLELAAAEPGRFVVIDAREPIESIALAIRERVEPLLPSPA"
     gene            complement(12140..14845)
                     /gene="topA"
                     /locus_tag="PLW15_02090"
     CDS             complement(12140..14845)
                     /gene="topA"
                     /locus_tag="PLW15_02090"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT33222.1"
                     /translation="MKLVIVESPTKAKTIAQYLGEGYEVLSSVGHIRDLIEPKNLPPE
                     LKKGSLGKFSVDVENGFEPYYVVSDAKKKTVAELKRALKGADELYLATDEDREGEAIA
                     WHLLQELKPKVPVHRMVFHEITKDAIQYARDNTRQIDTALVDAQETRRILDRLYGYEV
                     SPVLWRKVGPGLSAGRVQSAATRLVVDRERERLAFRTASYWDLIAGLTPKEEKDRFES
                     KLVRIDGKRIAAGRDFDDKGQLKGDAVALDETAAATLAGALREHDSVVVSGVESKPYT
                     RRPAAPFTTSTLQQEAGRKLRFSARQTMSVAQSLYENGHITYMRTDSPTLSQQAIEAA
                     RSQARKLYGAETVPDAPRLYTGKSKNAQEAHEAIRPSGDTFKTPSELSSVLRGNDFKL
                     YDLIWKRTVASQMADAKGSTATVTISASTASGTVAEFVASGTVITFRGFLHAYEESQD
                     EERNAAEAAEPAEAKLPPLTEGQKLVLVDVEAKGHETSPPPRYTEASLVKQLEELGIG
                     RPSTYASIISTIIDRGYVTPRGQALVPNWIAFSVVRLLEEYFSDLVQYDFTAGMEDDL
                     DRIADGEADRVEWLTGFYFGSPKHRGLRTVIDNLGDIDAREINSIKIADDVTLRIGKY
                     GPYLEAPGEDPETPRRVNIPQDLAPDELTAEKARELIDAPVVGDRVIGVNPETGKEIV
                     AKDGRFGPYITELEPEAPVVEPVETIDPKTGEVKIKKPKKVAVVKPRTASIFKSMDLA
                     TVDLDTALALLNLPRVVGTDPESGVEITAQNGRFGPYLKRGTDTRSLATEELIFDIDL
                     PGALELYSQPKYGGRAASSALKEFELPDPVSEKPIKIKDGRFGAYVTDGTTNATIPRG
                     EQLEDIDFDRAVQLLADKRAKGPAKPKAKATTRKPAAKKAPAKKA"
     gene            complement(14930..15283)
                     /locus_tag="PLW15_02095"
     CDS             complement(14930..15283)
                     /locus_tag="PLW15_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flp pilus-assembly TadE/G-like family protein"
                     /protein_id="HOT33223.1"
                     /translation="MTEHRGDRGSASVVIVGLMAGIAALTSLAVPLYSALAMKQSVAA
                     AADAAALAAADVAVGRAPGFPCEVATRVAAANGASVTACSVDGLVATVSASRFLLGIP
                     VVSTATAGPPPGEPD"
     gene            complement(15280..>15471)
                     /locus_tag="PLW15_02100"
     CDS             complement(15280..>15471)
                     /locus_tag="PLW15_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33224.1"
                     /translation="AHAARSLARGESADVAAARAAREVPGASITQSTDGDLTCVTGSA
                     PAALGLTVTASSCALSGGR"
BASE COUNT         2369 a         5131 c         5403 g         2568 t
ORIGIN      
        1 cctcgccctc atcgagcgct tctacgaccc gaacgcgggc gtggtgcgtc tcggcggcat
       61 cgacattcgc ggcctcgacc gcacggatct gcgggcccgg atcggctacg tcgaacagga
      121 cgccccggtt ctggcgggca cgatccgcga gaacctgcgg ctcgccacac ccgacgcgac
      181 cgacgagcag tgcatcgagg tactgcacgc cgtgaacctc accgaggtgc tggagcgcaa
      241 cgaactcggc ctcgacgccc cggtcggcga ggacggcgtc atgctctcgg gcggtgagcg
      301 ccagcgactg gcgatcgccc gcaccctgct ggcggccccg cccatcctgt tgctggatga
      361 gtcgacctcg agcctcgacg gcctcaacga gcagatgctg cgcgaggcga tcgatgcggt
      421 ggcccagaac cgcacgctca tcgtgatcgc gcaccggctg tcgacggtcg tcgactccga
      481 ccagatcgtc gtgctcgatc acggacaggt tgtgggggtg ggtacccact ccgagctggt
      541 caagtcgacg cccctctaca aggacctcgc gaagcaccag ctgctggttt aggcgggcgc
      601 ggtctcgtcg atttcgccgc cggccaacct cgcctgtcgc ctgcgctggc cccttctgtt
      661 gtgcaggaga agcgactatt gctcgcgcct cctgcagaac gggagtctgc ggaaggaggg
      721 agtcgacatt tcgcggccgg cacccaacag acgcggcggc ggcacccgag accccacggc
      781 cagcacccga cgccccgcgg cggcacctag cccgcgagcg catacatcgc gacggccgct
      841 gtggccgcca cgttgagcga atcgatgccg tgggccatgg ggatctgcac gacgctgtcc
      901 gcggcatcca gcccctcgcg ggtgagaccg tagccttcct cccccagcac aagggcaacc
      961 cgctcgggtg cggatgccgc gaactcccgc aggtcgactg cgccgggctc gagcgccaac
     1021 gcggccacgt ggaacccggc tgccgtcaag acctcccgcg cactgcccca gtcgccgagg
     1081 cgcgtccacg gcacctgcag caccgttccc atcgacactc ggatggcccg ccggtagaac
     1141 gggtccgagc accgcggcgt gaccagaaca gcgtccgccc cgatccccgc gaccgatcgg
     1201 aagatggcgc cgacgttggt agggtctgcc acgttctcga ggatgacgat gcggcgggca
     1261 tcccgcagca gcgactcgac cgtaggtagg gcggggcgat gcatcgaggc gatcagcccc
     1321 cggtgcagca ggtagccggt cagttgctcg agcagttcgg gcgggccgtg gaagacgggc
     1381 acgtcgtgcc cggcggtcag ttcgagcgcg tcgctgaccg aggagccgag cgcgagcacc
     1441 gatcgcggcc ggtgcccggc tcgcagagcg cgcccgagca cgagcgccga ctcagcgatg
     1501 tagagcccgt gctcggtgcc ctgcgccttc ttgagtgcga catcagtctg gtgcgagtag
     1561 tcggcgaggc gcgggtcgtc gagcgagtcg atctcgatga cggtcatcgg tatcgacttc
     1621 gctcgaccac cgctggcatc aggcgaacgg gttgggcgtg agcacgtact tcgtctcgag
     1681 gtactcgtgg atgccctcga agccgccctc gcggccgacg cccgactgct tcatgccgcc
     1741 gaacggtgcg gccgcgttgg agacgacgcc gacgttgagg cccatcatgc cggtctgcag
     1801 cgtctcgatg agtcgctgcc cgcgcgcgaa gtcctgcgtg tagacgtagc tcacgaggcc
     1861 gtactcggtg tcgttggcct tggcgacggc atccgcctcg tcggtgaagg tcgtgatcga
     1921 cagcaccggt ccgaagatct cctcgcgcag aatctcgctg cccgggttga cgccggtgat
     1981 cacggtcggc tcgtagaagc tgccgtcgcc ctcgactcgc tttcccccgg tgaggatgct
     2041 ggcacccttc gtcaccgcgt cctggactag cgcatctgcc ttgtcgacgg cgtcgtcgtc
     2101 gatgagcggg ccgatgatga caccgggctc ggtgccgcgg ccgatcggga acgccgtgac
     2161 gcgctcggtg acccggcgcg cgaactcgtc ggcgacggac tcatgcacga tgaaccggtt
     2221 ggcggcggtg caggcctgcc cgatgttgcg gaacttggcg agcatcgcgc catccaccgc
     2281 cttgtcgagg tcggcgtcct cgaagacgac gaagggggcg ttgccgccga gctccatcga
     2341 ggtgcgcagc acaccctctg acgcctgctt catgagctgc tggccgacac cggtcgagcc
     2401 ggtgaagctg agcttgcgca ggcgcgggtc ggcgatgatc gggtcggaga tcgcccggct
     2461 cgtcgaggtg gtgagcacgt tcacgacgcc cttcggcagg ccggcgtctt cgagcagctt
     2521 cgcgaagtac agggtggtca gcggcgtgag cgccgccggc ttgatgatgc tcgtgcagcc
     2581 ggccgcgatt gcgggagcga tcttgcgggt cgccatggcg agcgggaagt tccacggcgt
     2641 gatgaggaag ctcggaccga ccggcaggtg ggtgacgatc atgcgcccgg tgccctcggg
     2701 gttcgagccg taccggccgg tgatacgcac cgcctcctcg ccgaaccagc gcaggaactc
     2761 gccgccgtag gtgacctcac cgcgggcctc ggccaggggc ttgcccatct ccagggtcat
     2821 gagcagggcg aactcgtcgg cacgctcctg cagcaggtcg aaggcgccgc gcaggatctc
     2881 gccgcgcacg cgaggcgcgg tggcccccca gctcgcctgg gtgtcggccg cggcatccat
     2941 ggcagcggcc gagtcggcca ccgaggcatc cgcgatggtc ttgatgacct tgccggtcgc
     3001 ggggtcgtgc acgtcgatcg aacggcccga cgtggactcg acccactcgc ctccgatgaa
     3061 cagacggtcc ggcacctttg ccagcagttc gctctccgaa atcatttcgc tccttagttg
     3121 ttgtacgtca gtggttggtc gtcacgacag gatgacgcct gccgaagcca tcgtctccag
     3181 ggccgccgcg ctggagaccg gagcgacacc ggcaaccagt tcggtgagca cccgaaccct
     3241 cctgccagcc ttgcgggcgt cgagcgccga ggcaagcacg cagtagtcgg tggcgatgcc
     3301 cacgacgtcg atgtccgtga catccagtcg atcaagaagc tcgggcaccg tctcgccggt
     3361 gtcggtgacc ccttcgaaga tcgagtacgc gggcttgccc tgacccttga gaacgtgcac
     3421 gtcgatgagc gcatcctcca gcgcggggtg gtactcggca ccctcagttc gggacacgca
     3481 gtgctcaggc cagctgtcga catagtccgg tgccgggtgg aagtggccgc cgttgtcgtt
     3541 gtcgccgtcg tgccagtcgc gtgaggcgat cacggcgtcg tagcggtccg gatgcccgtg
     3601 cagcagtttc gtgatgccct cggcaacggc cgccccaccc tcgaccccga gggcgccacc
     3661 ctcggtgaag tcgttctgga cgtcgatgat gaacagcgcg cgcgacatca gttgttcgag
     3721 aggttgtcgc cgcagctgta catggcggtc gccagggtgt cctgggcctc cttggcatcc
     3781 tcggtgatgc cctcgcggat cgagtagaag gcgaacgcga gagtcgagcc gtcggccgcc
     3841 ttgatgattc ccgcgagcga atactcggtg tcgagccatc cggtcttggc catcacactg
     3901 ccggcagcga tcgcattcgg tccgttgaat cgggaggcta gcgaccccga gacacctgcc
     3961 accggcagcg acgagtacag aacaccgagg tccttctccc cggcgttcac cttggtcagc
     4021 agtgcggtga cgaactgcgg cgagacagcg ttcagatcgc tgagcccgga gccgtcgcgc
     4081 accgtgacct tggtggtgtc gaggccgtag gcgaccagcg cgcccggtat cgcctgagcc
     4141 agcgaggtgg atgacccgtc gaagcccatc gccttggaga ccacccgcgc gagcatctcg
     4201 gcgagaacgt tgtcgctgga gagcagcatc ttgtcgatga ggctgctcac cggctgcgat
     4261 ttcacttcgg cgagcaccgt actaccgacg gccgcccccg aggagaacgt cacgccgaac
     4321 aggcccgccg cgtcggcgaa ggcgcgcccg gcatccccga ccgggtcggt cgtgcggtag
     4381 ctgacgttgg cgctcggatc ggcccggccg ccatcgacca tgagcgcggt gacctgagcc
     4441 tggtacccgt cgtcctgctc cttgcgcagc caggaggaat cccagttgtc ggcagggtcc
     4501 cacatggtgg agtccaagac gatctcggtg atcggcaccc cggggtgaac ctgttcgtac
     4561 ttcgccatcg ccgcgtccgc gaggtcggca atcagtggcg caccgtcata gacggtgttg
     4621 gaggtggtgg ccagcgtggg gtcacctccg cccttgagca cgatcacccc agggctcgac
     4681 ccgtcgatca cttgggtggt cagctgcccg ccgggcccga ggatgttgag tgccgccgcc
     4741 gcggtcagca ccttgagcac gcttcccgtg cgttgcggca acgtgccgct gtggtcgaag
     4801 agcgtctcac cggtggcggt gttgacgacg tatcctgcga agtcggcgag gcgcggatcc
     4861 gccatgacgc tcgccacaga gcaggtacgc agccggctgg cgaccggaac ggccgacggt
     4921 tgcggccgag ggtccgccgt ctcagtgggc aacggtgccg ctaccgtcgg cccaccgccg
     4981 gcggccacac ctgcgaacac tgctgacgtg ccgagcagca ggaacaccac ggcgatcgcc
     5041 gagatgagcc acgcggtcgg atgcttgcgg atgatcgccg agatgcggcc cggcgccttc
     5101 ggttcaggag tgcgcaggga ccgcctcgac agcgcctgcg taggcaccga gtcgttcgaa
     5161 tccgtcaccc ggtaatgata cccgggctgc cgattggttc tctgggtgga cgcctagcag
     5221 taggtccagc gagcagctgc ccgggtgaac gagtacggct cgtcgattcc accgtgggtc
     5281 atcgcgaagg cgatgcagac accacccgtg ccggcacccg gtcggtagag cgacgcctgg
     5341 tgcgatgacg actgccacca cttgatcgcc actgtcgcac cgctgttgcc cgaaccgccg
     5401 gcgaggttac cgtcgcagcc cacgctgggc acgccgtcga tgcgcaccga gcccatgtga
     5461 ccccacgcgt ccatcgggtc ggggctgggc gactccgcca tccagaacag acgggcttcc
     5521 atgcaggagt cgtagacgaa atagctcacg ggctccaggc agttctccgc gcggatcgcg
     5581 ttgtactggt acgcgaacga cgcgaggtcg tcggaggtgg ttccgggaac gccgagtgcc
     5641 gagaccgcgc cgggggcgcc gctcgtgccg cccgcgctga atccggggca ggagagtccc
     5701 tgcgcctcga gcgcagcgcg gcgttcgttg gtcccggatg ccgcacccgc agcatccacg
     5761 ggaggaagag gcttgggtac cggcttctgg gccaggtgct cccaatcgct ggtgagggac
     5821 ttcgactctg cgacgatccg cgcctcgatg acctggtcgc ttccgctgta gcgcagcgcg
     5881 gcctggtcgg cgggctgaga ctgttcggca gcgatgaccg aagcgagcat gggtgccgcg
     5941 gcaaccgatg gatccgcgac ggcgaggccg gtgagtccgg gtccgccacc gcccacgaac
     6001 ggcagcgcta cgatcacagc ggtcgagaag accgtcgcca aaaatgcgta actcttcatt
     6061 ttcgtgcacc cccacgcact acgccgaaca cccccaattg gttacgacgt ggtgccaacg
     6121 tacgtctgcg tcaccatccc cgcaaccccg ctggtgcgca aaacgaacat tccacccgcc
     6181 ttttgggggc acagtgagga ggctttgtcc cgcataagga ggacacgatg ggtagtggag
     6241 ccgcctaaga gaatcgaact cttgaccttc tcattacgag tgagatgctc taccgactga
     6301 gctaaggcgg ccggcccggg tcgccccgag gcacagcatg aaagcttagc cgagaattgt
     6361 cggccgcttc gcacgggttg ggctcagacg aagagagtct tcagctcggc gaacgaccgg
     6421 cgcagctgca cgccgagctc ggttctcggt tcgctggttg cccagctcgc ccaggcgtgg
     6481 cgaatgacgc cgagcgacac atgggtcatc agccgtgcgc ggcgctccag cactgatgcc
     6541 tcagcgtcgg gctggtcgtg acgaagccgt cgcacgatga ccgacgcgac ccgctcctcg
     6601 aagtcgcgca tcttggccat gcgcagggcg aagagctgcg gatactcctt caccagcgca
     6661 tgacgtagct gcatcatctc ggcgtcttcg agagagctgt ccccgacctc gatgagcacc
     6721 gcgacgagtc cgtcgaacac gccgctgttg tcagcgttcg tgacgaaccg ctcgaccgta
     6781 tcagtctcgg ccagagtagg cgggtcgccg aggatcgcct cctccttcga cgcgaagtag
     6841 ttgaagaagg tgcgcgggga gacatccgcg atcctgctca cctcgtcgat ggtgacgttg
     6901 tcgagtcccc gctccgcgac cagctgcaat gcggcgagct ggatggcacg gcgagtcgca
     6961 cggcgcttgc gctcgcgcag ccccggctcg gcggcatccg tcgcgatggt catgaatcta
     7021 ttcttgcaca ctgcgcaaaa ttgcgcggcg agaggtcagc agttcggatc ggccgtcgga
     7081 acgacgccct tgagcagata ctgatcgaca gtggaggtga tgcactcgtt gccctgcccg
     7141 taggcggtgt gcccctcgcc gttgaacgtg atgagcacgg cgctctcgag ctgatcggcc
     7201 agtgccacgc cccactcgta gggcgtcgcg gggtcacccg tcgtcgagac cacgagcacg
     7261 ggcggtgcgc ccttgccggt cagtgcggcg atcggcggac cctggtactg gtagggccag
     7321 ttgatgcacg tcgtgtcgcc gatcggcggc gacggccggt aggtggtcgg cgacgcctgc
     7381 tcgagcagca cctcctgctc ggcgagcacc gtcgggtcgg tctcgaccgg gtaatccacg
     7441 cagtagatag cgaggaaagc ctcgagcgag ttgtcggcgt aggtgccgtc ggtgtcgcgg
     7501 ctgtagtagt agtcggcgag gaagaatgcc gtgtcggtca cgccgtcctg cgcctccgag
     7561 aacaggtcgt tgaggaacgg ccacgagtcc tgcgagtaga gggccatgct catggcgatg
     7621 tcgagcacgc cggcatccat catgcgtccg tcgggagcgg ccagcgggga tgcgtcgtac
     7681 cggtcgtaca gcccgcggat cgtcgcgagg tccgtcgtca ggtcacccgt gaacgggcag
     7741 tcatcgaaca ggtccgggca ggccttcagg tagttctcga gcgcgcgctg gaatcccttg
     7801 gtctgcacga gccccacctc gaacacggag atcgaggagt ccgtcgcgcc gtcgacgacc
     7861 aggcgaccga ccttgtcggg gaagttctcg acgtagtacg agccgatgtc gctgccgtag
     7921 gagtagccga ggtagttgag cttcgggtcg cccaccacgg cccgcagcat gtcgaggtca
     7981 tgcacggtgc tctgggtgtc gatgaactgc agcagctcgc ccgtgttctc gagacacgcc
     8041 ttgccgaagt cgatcgagga ctgggtcacc tcgtcgatcc acgcctgggt gccgcgcggc
     8101 gcctggggca ggccgaagat gaacgcgtcg agctcgggag cctcgtagca cttcacggcc
     8161 gacgaccgcc cgaccccgcg gggatcccag ccgacgacgt cgaacgcggc ctgcaggtcg
     8221 gcactggccg cgaagtcgag cgagtcgtag acgaagtcga agccggatgc tccggggccg
     8281 ccgggattgg tgaagagcga gccctgcttg gttccggtgg cggggtggcg cacgacggcg
     8341 agttcaatgt cggtctcggg cgacgggttc gcccagtcca tgggcgcctt ggccgtcgcg
     8401 cactccatgc cgttgtcgca gtccgcccag tcgagaacct gggtgtagta cggaaccaac
     8461 gcggccggca cgtcctcgcc ggtcggtttc gtggtgttgt cgggctgcag gacgccgaag
     8521 ttgaagcaac ccgagagcag gagaagcagc gccgagccgg tggcgatgag cgcggtggcg
     8581 cgaaaggatg ccgtgttcac agcccgagcc tatcggtgag cccctcgggc ggtcagcact
     8641 tcgggtcggc cgccggcaca gttccgttga tcaggaagtc atcgacagtg tcattcacgc
     8701 aggcgttgga cttgttgtac gccgtgtgac cctcgccttc gtacgtgacg aggtgcccct
     8761 tcgacagggt gttcgcaaca gtcaccgccc acgagtacgg ggtggccggg tcattggtcg
     8821 tgccgatcac gaggatgtcg ctcgacccgt ctgcgacgat cggcgcgcgg tcgcgcttgg
     8881 ccggcaccgg ccagttggga catcccgttc cgccccacga catctgcggc ccgaagaccg
     8941 gggcaagctg cttcagctcg gccgcctcgg cgcgcatcgt cgccacatcg ccatcgccgt
     9001 gagcatccag gcagttgatg ctgatgaacg cctcggtctg gttgtcacga taggtgccgt
     9061 cttcgttgcg accgttgtag ttgtcggcca actggaaggc gtaggtcgcg tcacccgcga
     9121 acacgtcggt gaacaactgc cgcaggtact gccagttgtc cttgttgtag agcggaagga
     9181 tgatcgcggt gaacatcgcc gagctaccga gctggcgacc gtcggagttg cgcagggggc
     9241 tcgcgtcgag gttgtcgagc agggcgcgga tctcggtgag cgagtcatcc acgctgccac
     9301 tgaacgggca gtcggatgcg cccgcgcagt cagcgaggaa cgcctttagc gcgctctcaa
     9361 aacctttcgc ctgcgtcgcg gtgccctcga agtcggtggc ggccgggtcg acggccccgt
     9421 cgagcacgag acggccggtc ttgtcgggga agaggtcagc gtagacctgc ccgagcaggg
     9481 tgccgtagga gtagccgaga tagttgaggg tggtgtcgcc cagggcggcg cgcagcatgt
     9541 cgaggtcgcg cgcggcgctc ggggtgtcga cgaggcccag cagcggaccc gtcagctcga
     9601 ggcagcgctc accgaacgcc ttcgagttcg ctcccagctc ggccagccac tcgtcgctgc
     9661 ccggctcgcc gggcacgatg tcgtagatgt aggcgtccaa ctccgccggg tcggtgtagc
     9721 acgtcaccgg cgtcgagcgg ttcaccccgc ggggatcgaa gcccaccacg tcgaagctgg
     9781 cctgcaggcg ctcgtcggtc gcgtagtcga cgctgtcctt cacgaagtcg tagcccgagc
     9841 cgccggggcc gcctgggttg accagcagcg accccacccg gttgccggat gtcgcgggct
     9901 ggcgcaccag cgcgagctcg atgtcgcccg cgctcgggtc gctccaatcc atcggggcct
     9961 tggccgtcgc gcactgcatc ccgccgccgc agctcgacca ggtgagcacc tgcccgtagt
    10021 acggcttcag ttcagccgcc acatcctcgc cggtgggcgt cgacgtcgat gcggccggtg
    10081 gcaggaacca cgacacgcac ccgctcagcg gcagcgttgc ggccagggcg agggcgacca
    10141 atcccagacg gagacttctc actgagctgc ggcctttctg acggcggtga caagcatggc
    10201 ctcgagcgcg agggccggtg cgacgttgct gtcgatgcga cgccgtgcga tggcgatggc
    10261 atccatggtg gcaagggttc ctgctgcatc gctgagagcg gcggcggctt cgacccggcc
    10321 gaggatgctg aggttgatcg ggtcgccggg cacgccgagc tggatcagca ggatgtcccg
    10381 gtacaacgac agcaggtcga ccatgatgcg gtcgatcccg tcgcgcaggc tgcgggtcgc
    10441 gcgccgcttc tggtcctcct cgagggcttt cagctggctg cgcagggctg gcgggatggt
    10501 gccgcccggg tcgattccga gcgagcgcag cgcggcgtca cgttcctcgg aatcgcgctc
    10561 ctcggtgatc gcctcggcgt ccgccttggc caggtcgaga agcaccgtcg cggcattggt
    10621 cgcgtcagac accgagcgga tgcccagcgc cacatccagc gtgcgctcgc gccgctggcg
    10681 ggcctgaaca ttggtggcga gtcggtgtgc catgccgatg tgcgactggg cttctctcgc
    10741 ggcgcgggtc gcggtcgcgg catccacccc gtcccgccgt tcgagcaggc ttgcgacctc
    10801 gtcgacgctc ggcacgcgca accgcacggt gcgcacccgg gaccggatcg tggggatgag
    10861 gtcggcctcg ctcggcgcgc acaggatcca cacggtgcgc ggcggcggct cctcgagtgc
    10921 cttgagcagc acgttggagg tgcgctcggt catgcggtca gcgtcttcga tcaccatgac
    10981 gcggtagcgc ccgaccgacg gcgagaactg cgaggtggcc accaactggc gcacctcctc
    11041 gatcgagatg atgacgcgtt cggtggcgag caccccgagg tcggggtggg tgcgggcctg
    11101 cacctgccgg cgggtcgcct cgtcgtcacc gccgagcagg gccgtcgcga aggcgtaggc
    11161 gagattggag cgccccgacc cgggcggacc ggtgatgagc caggagtgcg tcatcgacga
    11221 gtcgtcgctg ccaccgaccc gggatgccgc ggccgccgcc tcgacgacgg cgatggcgtc
    11281 ggcctggccg gtgagctcat cccagattgc cacgcgctca gcctagatca ggccgacgac
    11341 ggctcaagcg cacactccgc cgtctcgcgg cgggtgagcc tggatgctgg gggcaccgcc
    11401 tgatcgcgga tcaggatgcc gcggcgacac gccgcgccgg cgcgcggcaa taccggcgtg
    11461 tcgccgcggc atcctgatct gcgaaagggg gcgggcacag tggcgcgtgc acacggagat
    11521 caggccggcg acggcagcag aggctcgacc cgctcgcgga tcgcgagagc gatcgactcg
    11581 atgggctcac gcgcatcgat cacgacgaag cgcccgggct cggccgcggc caactcgagg
    11641 taggcctcgc gcacccgggc gtggaagtcg tcaccggcgg cctcgagtcg gtcgtacttg
    11701 gtgcggtcgg ccagccggtc ctgcccctcg tgcaggtcga ggaggatggt cagatcgggc
    11761 agcagcgact cggtggccca gagcgagaca tcccgaacct cgtctgcacc gagcactcga
    11821 ccggcgccct ggtaggcgac cgaggagtcg aggtagcgat cctgcaccac gatgtcgccg
    11881 cgctccagtg cgggacgcac cttggtggcg atgttgtgcg cgcggtcggc cgcgtagatg
    11941 agagcctcgg cccgcggggc gatctcgccg cggcggtgca gcacgatctc gcgcagctcg
    12001 agcccgaggt cggtgccgcc gggctcgcgc gagacgacga ctgtgcgacc ctgcgcctcg
    12061 agccactgca ccagcagctc ggactgggtc gacttgcccg acccgtcgcc gccctcgaag
    12121 gtgatgaaca ggccgcgcat caggccttct tcgccggagc cttcttggcg gccggcttgc
    12181 gggtcgtggc cttcgccttc ggcttggccg gacccttcgc ccgcttgtcg gcgagcagtt
    12241 gcacggcgcg atcgaagtcg atgtcctcga gctgctcgcc gcgcgggatc gtcgcgttcg
    12301 tggtgccgtc ggtcacgtag gcgccgaagc ggccgtcctt gatcttgatc ggcttctcgc
    12361 tcaccgggtc gggcagctcg aactccttca gagcactgga tgccgcccgg ccgccgtact
    12421 tgggctgcga gtacagctcg agcgcgcccg gcaggtcgat gtcgaagatg agttcctcgg
    12481 tggccagcga ccgcgtgtcg gtgccgcgct tgaggtacgg accgaagcgg ccgttctgcg
    12541 cggtgatctc cacgccggac tccgggtcgg ttcccaccac gcggggcagg ttgagcagtg
    12601 cgagggcggt gtcgaggtcg acggtcgcca gatccatgga cttgaagatg gatgccgtgc
    12661 gcggcttgac gaccgccacc ttcttcggct tcttgatctt cacttcgccg gtcttcggat
    12721 cgatggtctc gacaggctcg accaccgggg cctccggctc cagctccgtg atgtatggcc
    12781 cgaaacgacc gtccttggcg acgatctcct tgccggtctc cgggttgacc ccgatcacgc
    12841 ggtcgccgac gacgggcgcg tcgatgagct cgcgtgcctt ttcggcggtg agctcgtcgg
    12901 gtgcgagatc ctgcgggatg ttgactcggc gcggcgtctc ggggtcctcc cccggtgcct
    12961 cgaggtacgg accgtacttg ccgatgcgca gggtcacgtc gtctgcgatc ttgatcgagt
    13021 tgatctcgcg cgcatcgatg tcgccgaggt tgtcgatcac ggtgcgcagg ccccggtgct
    13081 tggggctgcc gaagtagaag ccggtgagcc attcgacccg gtcggcctcg ccgtcggcga
    13141 tgcggtcgag gtcgtcctcc atgcccgcgg tgaagtcgta ctgcaccagg tcggagaagt
    13201 actcctcgag caggcgcacg acgctgaagg cgatccagtt gggtacgagc gcctggccgc
    13261 ggggcgtgac gtagccgcga tcgatgatcg tggagatgat gctcgcgtag gtggaggggc
    13321 gcccgatgcc gagctcctcg agctgcttca cgaggctggc ctcggtgtag cgcggcggcg
    13381 ggctggtctc gtgacccttc gcctcgacat ccacgagcac gagcttctgg ccctcggtca
    13441 gcggcggcag cttcgcctcg gctggctcgg cggcctcggc ggcgttgcgc tcctcgtcct
    13501 ggctctcctc gtaggcgtgc aggaagccgc ggaacgtaat gaccgtaccg ctggccacga
    13561 actcggcgac ggtgccgctc gccgtggagg ccgagatggt gactgtcgcg gtcgagccct
    13621 tggcgtcggc catctgggat gcgacggtgc gcttccagat caggtcgtac agcttgaagt
    13681 cgttgccgcg cagcaccgag gagagttcac tcggcgtctt gaaggtgtca cccgacgggc
    13741 ggatcgcctc gtgggcctcc tgcgcgttct tgctcttgcc ggtgtagagg cggggcgcat
    13801 ccggaaccgt ctcggccccg tagagcttgc gggcctgcga gcgcgccgcc tcgatcgcct
    13861 gctgcgacag cgtgggcgag tcggtgcgca tataggtgat gtggccgttc tcgtagagcg
    13921 actgcgcgac gctcatggtc tggcgggccg agaagcgcag cttgcggccg gcctcctgct
    13981 gcagcgtcga ggtggtgaat ggggcggccg ggcggcgcgt gtacggcttc gactcaacgc
    14041 cgctgacgac gaccgagtcg tgctcgcgca gcgcaccggc gagcgtcgcg gcggcggtct
    14101 cgtcgagggc gaccgcgtca cccttgagct gacccttgtc gtcgaagtcg cggccggcgg
    14161 cgatgcgctt gccgtcgatg cgcacgagct tcgactcgaa gcggtccttc tcctctttcg
    14221 gagtcagtcc cgcgatcagg tcccagtagc tggcggtgcg gaacgcgaga cgctcccgct
    14281 cgcggtcgac gaccaggcgg gtggctgcgg actgcacgcg gccggcggag agaccggggc
    14341 cgaccttgcg ccacagcacc ggcgacacct cgtagccgta gaggcggtcg aggatgcgcc
    14401 gggtctcctg cgcgtcgacg agggccgtgt cgatctgccg cgtgttgtca cgggcgtact
    14461 ggatcgcgtc tttggtgatc tcgtggaaca ccatgcggtg cacgggaacc ttcggcttca
    14521 gctcctgcag caggtgccac gcgatggcct caccctcgcg gtcctcatca gttgcgaggt
    14581 agagctcgtc ggcgcccttg agtgcgcgct tcagctcggc gaccgtcttc ttcttcgcgt
    14641 cggagaccac gtagtagggc tcgaacccgt tctcgacgtc gacggagaac ttgcccagcg
    14701 accccttctt gagctcgggc ggcaggttct tgggctcgat gaggtcacgg atgtgaccga
    14761 ccgacgacag cacttcatag ccctcaccca ggtactgggc gatcgtcttc gccttggtgg
    14821 gcgactcgac gatgacgagc ttcatggaca caggtctcct tggatagaac gccgggcggc
    14881 tgtggtggag gcgtcagcga caccatacac acactcgttg cgctctcacc taatccggct
    14941 cgccgggcgg cggaccggcc gttgccgtgg aaacgaccgg aatacccagc agaaagcggc
    15001 tcgcactgac cgttgcgacg agcccgtcca cgctgcaggc ggtgacggat gccccgttgg
    15061 cggcggccac gcgggtcgcg acctcgcacg ggaaccccgg cgccctgccg acggccacgt
    15121 cggcggcggc gagcgctgcg gcatccgctg cggccgccac ggactgtttc atcgccaacg
    15181 cggagtagag aggtacggcc agtgacgtga gtgccgcgat gcccgccatc aggccgacga
    15241 tgacgacgct tgccgacccg cggtcgccgc gatgctcggt catcggccgc cgctcaacgc
    15301 gcaactgctg gccgtcaccg tgaggcccag cgcggctggc gccgagccgg tcacgcaggt
    15361 gaggtcgccg tccgtgctct gggtgatgct cgcgccgggc acctcgcgcg cggcgcgggc
    15421 ggcagcgacg tcggccgact caccccgggc gaggctgcgg gcggcgtggg c
//
LOCUS       DAOSOU010000021        15436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3848_length_15436_cov_5.722060, whole genome shotgun sequence.
ACCESSION   DAOSOU010000021 DAOSOU010000000
VERSION     DAOSOU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3848_length_15436_cov_5.722060"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLW15_02105"
     CDS             <1..232
                     /locus_tag="PLW15_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT33225.1"
                     /translation="EFAEFADSQGLTPPVAALAWVSQLPGVTTVIPGARNVRQAVSNA
                     SAGSVPQLTPEFTEQITELYDRTFREAIHPRW"
     gene            226..1497
                     /locus_tag="PLW15_02110"
     CDS             226..1497
                     /locus_tag="PLW15_02110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33226.1"
                     /translation="MVTSSRGGLGRPFANLFTANLASSLGDGIARTAIPLLAARLTSD
                     PFLVSVVAALALLPWLFFAIPAGILIDRIDRRRALAMAQAVRTALAVLLIGLVATDSL
                     TIWWLYIVVFVYGMFETVYDGAIRAVVPSIVDRANLPRANSRIEGGELVVQNFVSGPV
                     TSALFAVSVLVPLGINGLVFAVAGALALALPRVASGRQFVAHGETQVAWYRQFADGLR
                     FIWANRMLRTLWLFTTFIGLATSAATATFVLYLLNRLGIPEAAFGLFMLSGAVGGIIG
                     AVLANRLKDAWGAGLTMAVMTTLCGVAFAFIGLVPSIWAAAVGFAVSSLAITIWNVLI
                     MSLRQSLVPGHLLGRVHGTWRTLLWGTMPLGSLIGGLIARVDLGLPFIIGGGAAAVAS
                     LIFFAFLRTLPNPEDVELPQGPGPTDPLVGE"
     gene            complement(1453..2208)
                     /locus_tag="PLW15_02115"
     CDS             complement(1453..2208)
                     /locus_tag="PLW15_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33227.1"
                     /translation="MIDLPQRHWGERGPRVLLVHGLSSTAESWWRVGPGIADAGFRVT
                     AVDLRGHGEAPRAERYALVDYAGDLPGDDWFAVIGHSLGGAAAVLAAQHPGFTRALAL
                     LDPVLLVTRDEWQATIDDQLSELGLDEQALGALKPHWHERDRAAKLAGIRQTDAHVIE
                     HSFTDNDRWDVTAEATALAVPTLLLRGDPAVYSMLSDETADALAAANPLVDYRVVADA
                     GHSPHRDRPEETIAALIGWLLADERVGRPGSLG"
     gene            complement(2243..2836)
                     /locus_tag="PLW15_02120"
     CDS             complement(2243..2836)
                     /locus_tag="PLW15_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT33228.1"
                     /translation="MPKVTDEYRIAKREEIADAALRAFRRKGFQGASMADIIAESGLS
                     AGAIYGHFASKGDIILDVATRVVGARVDDVQQLMASDPLPPPSHLLRVLMGGMLEDLG
                     DPTILVQLWGEAATDPALRDLAQGVFAQLQGAYRRYISLWHQREHGVSEEEGDAIGAE
                     QVSLFVSGAQGFIVQAALMPDFDRERYLTTLDKYLPR"
     gene            2928..3968
                     /locus_tag="PLW15_02125"
     CDS             2928..3968
                     /locus_tag="PLW15_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33229.1"
                     /translation="MSTPALVAHPHTPFRRVVVVGVALAAVLSVILLAFLWPTVTSSV
                     KALPIAIAAPAEQADQLAGALEERSPGAFDVTTVADRATAVDLIETRQAYGAIVLGSD
                     PEVMTASAASPLVAQLLTGFAPALAAQLNAAAAAQGIALPAPLIVTVTDVVPLASTDA
                     RGLGLASSSFPLVLGGMIGGIAVSLAIVGVWRRVTATLIYSAVGGLAITGILQGWFGA
                     LQGDYFVNAAAVALTILGIAGTIVGCVSLFGRPGIAVGPVLFLFIANPISAAAQPVEF
                     LTAPWGAIGQWFPPGAAATLLRELSYFPRADTLFPWLVLAGWAVLGLLLSTLGHYRDR
                     GAATQAALAAAE"
     gene            4058..4618
                     /locus_tag="PLW15_02130"
     CDS             4058..4618
                     /locus_tag="PLW15_02130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT33230.1"
                     /translation="MTKLMIILGSVRPKRAGEAVAEWVRGAVAAHHHSFEVDFVDLRE
                     LALPFMDEPEHPAKKAYTQPHTIAWSQRVDAADAFILVSPEYNHSYSPALKNALDFLA
                     TEWKHKPVGLVSYGGVSGGTRGVAALDPVLATVGLVKTSHSVEIDYVSTQIVDGVFTP
                     AEKHAALLSHQLDDLTELSAVLATLR"
     gene            4662..5909
                     /locus_tag="PLW15_02135"
     CDS             4662..5909
                     /locus_tag="PLW15_02135"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HOT33231.1"
                     /translation="MSPTKRNVTAKWPGFTTDLLRGDGEHADRVSFLELFFDLVFVFA
                     VTQISHTLVHSIDDGHALDGGLQATVIILAVWWVWVYTAWVTNWLDPDKAAVRWMLII
                     LMVLGLLMSTSIPEAFGDRALLFVSAYVAMQVGRAIFTILSMVRFVPENALNVTRLTI
                     WFCVSAIFWFWGALTEDLTARLVLWGVAIVIEYLGPVSRYWVPGLRSSYIGGIPIRGG
                     HMSERAGLFVIIAIGESVLVTGTAFADHPIDLPNTLAFFASITGSILLWLLYFSRAES
                     GGSRFIRRHSNPSLVAANSYTYLHVVLVAGIVVIAVADELVLAHPIDAPRQLTLALVY
                     GAPALYLIGNLVFKKSIGAPWLKSHLAGVGILVIFGAVVLLSGLQLPALVHSWIANVV
                     LIAVVIGEEIDFRRERSRLRSLR"
     gene            5819..6304
                     /locus_tag="PLW15_02140"
     CDS             5819..6304
                     /locus_tag="PLW15_02140"
                     /inference="COORDINATES: protein motif:HMM:NF040366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6264 family protein"
                     /protein_id="HOT33232.1"
                     /translation="MDRQRCAHRRSDRRGNRLQTRTKPPSFATVSAMTDAVTPAPDPA
                     ATSATPRPRRTWDLILTIVLLIAYLLGTLVASFSAFFLAFAGDSCGASSVCDENQMAN
                     GMMIALYAVWIPALFVVIGAIVLLVLRRLAFWVPLVGGALTIIISIIGWAVTISAVRT
                     G"
     gene            complement(6336..7544)
                     /locus_tag="PLW15_02145"
     CDS             complement(6336..7544)
                     /locus_tag="PLW15_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33233.1"
                     /translation="MKSYIDLLKTPGVARILSAQLLARFPGGMLSLAYLLHIEHIFNS
                     YGAAGLVLAATSVGQAVSGPVTSRWMGLWAIRPVLILTTSICALSMATIAFVPLPLVG
                     YMIVGLIGGLSYPPIQPAVRTIYPKMVNSQQLTPLFSLDASAQEIIWIAGPVLVTFVS
                     IQISTVLGIVMAMAFLIFGGAWFISSPEVGRVRIPRSKRRLGVVLARPPVLLATVVGF
                     LLVGSCAAVEAGVVATFGEGGPVAGTVLALWAISSLAGGLLLGHIPVGPWALARRMIV
                     VAVGCALAIFAWDFWSLSAALLVAGLCIAPALAVMFAIVSASVKFSDTAEAYGWVGTG
                     QLIGAAIGSAVAGFLIDSAGAGGAFWTAAGFAAVGAIVPLVFRRAHPDLRGRDASPIP
                     DTEPVPIQPS"
     gene            7806..>9082
                     /locus_tag="PLW15_02150"
     CDS             7806..>9082
                     /locus_tag="PLW15_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOT33234.1"
                     /translation="MSITVVKRSGQREPYDANKINLAIEAATVGLDDNITWVTQIASE
                     LELTLFDGITTQQLDEAVIQVALQNVKDDPAFDVVAARLLLKTIYKRVLGDYETAEEL
                     KELHRSHFAPNVARGVEEGLLDPRLTEMFDLDELASHLEPAHDELLKYIGVVTLNNRY
                     GIKGRNGDALEVPQYFWMRIAMGLSLNEANPTETALKFYRKMASLEYLAAGSTLVNAG
                     TIYPQLANCFVMEMQDDMEHIAKTTRDVMWLTKGTGGIGLSVTKLRAQGSPIRSNNTT
                     STGPIPFMHTIDSILRAVSRGGKKFGALCFYMENWHLDFPEFLDLRQNSGDPYRRTRT
                     ANTAVWISDEFMKRVQNDDDWYLFDPLEVSDLNELYGKAFSERYNEYVAKAEAGEMRL
                     FKKITAREQFKAILISLQTTSHPWLTWKDTINNR"
     assembly_gap    9083..9182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9183..10278
                     /locus_tag="PLW15_02155"
                     /pseudo
     CDS             9183..10278
                     /locus_tag="PLW15_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146878.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     frameshifted; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
     gene            10281..11279
                     /locus_tag="PLW15_02160"
     CDS             10281..11279
                     /locus_tag="PLW15_02160"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HOT33235.1"
                     /translation="MGILGTGLQDGLLLKPIRYQWAMDLYDQAVANTWFPNEIQLGED
                     IADFKKMTDEERHAVTFLMSYFNPNELLVNKALAFGVYPYVNAAEATLYLAKQMWEEA
                     NHCMSFEYVLETFPIDREAAYGSHVNVPSMARKEEFEVKYIRRMTEQTLDITTTEGKK
                     DFVRNLVAYNVILEGVWFYSGFMVALSFRQRNLLRNFGSLMDWVVRDESLHLKFGINL
                     ILTVLEENPDLQTEEFATEIKQMILDAVEMEEAYNHDLLPNGILGLNATYINQYVKYL
                     ADRRLEELGFEAHYNVSNPAKWMAAANDTLQLVNFFESTNTSYESNASATVGAKSE"
     gene            11295..11855
                     /locus_tag="PLW15_02165"
     CDS             11295..11855
                     /locus_tag="PLW15_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33236.1"
                     /translation="MSSRDAAYRNPIRIEYVDWLDPRAVALREAMDAETSAMYAAFAS
                     GQSAEVTAAIADALTVDPAEIVTTIVAVDGDEPVGHSALRPVHLDTLDDRAAPDDRAA
                     PDDRVLEVKKVFVAESVRGRGVARLLMGELETVARGRGAASLVLQTGPLQVPAITLYE
                     DLGYVAIPPYGKYDAIPGALCFEKVL"
     gene            complement(11856..12611)
                     /locus_tag="PLW15_02170"
     CDS             complement(11856..12611)
                     /locus_tag="PLW15_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT33237.1"
                     /translation="MKIAGCSALVTGGASGLGLATALALAGRGAHVVIADLPSSRGTE
                     LAAEHGLTFVAADVTDEHSVARAVEAATELGPLRVAVCCAGVATAERAVGRDGPLPLE
                     RFERVIAINLTGTFTTVKLAAAAMMATEPIDGERGVIVMTASVAAFDGQIGQPAYAAS
                     KAGVAGMTLPLAREFAHTQIRVVSIAPGIFQTPMMSGLPQAAQDSLAAQVPHPSRLGR
                     PDEYAALALHIVDNAMLNGETIRLDGAIRMAPK"
     gene            complement(12612..13766)
                     /locus_tag="PLW15_02175"
     CDS             complement(12612..13766)
                     /locus_tag="PLW15_02175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT33238.1"
                     /translation="MSTAVIVDALRTPSGRGKPGGALSGEHPVDLLGTVLATLVARND
                     LDPALIDDVIAGCLSQIGEQSVNIARNAALAAGFPFSVPGTTIDRQCGSSQQAAAFAA
                     QGVIAGAYDVVIACGVELMSKNPINSATLGLDNWGPMVRSRFPDGLVGQGISAELIAA
                     RWNLSRAALDEFAARSHRLAAAASFESEIMPVGDFEADETIRPGTTAEGLASLKPAFE
                     DGALGERFPEIDWRITAGNSSPLTDGASAVLIMSEEKANHLGLRPRARFHSSVVVGSD
                     PIEMLTGILPATHKILDRSGFSIDDFDTVEINEAFASVPLAWQHEFEIDHNRLNPRGG
                     AIALGHALGSSGTRILTSMVNQLELSGGRYGLQVMCEGAGMANATIIERL"
     gene            complement(13806..14576)
                     /locus_tag="PLW15_02180"
     CDS             complement(13806..14576)
                     /locus_tag="PLW15_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT33239.1"
                     /translation="MTGRLDGKVAVITGTASGQGRAAAELFASEGAIVVGCDVDAAGS
                     AETVRLVTAAGGTITSTHPLDLTDDAAVRDWIAQVVADFGRIDILYANAGATRFAPVA
                     DTSYDDWAWVLRHELDIVFLPVHHAWPHLSEGASIVLVGSTAGITGSMTNTRIAHTAT
                     KGGVVAMTKQLAAEGASRGIRANCVSPGMILTPATQGDLLADDHPMRQIAQHIPLGRI
                     GQPIEVARAALFLASDEASYITGTNLVIDGGWSAVLPG"
     gene            complement(14573..15394)
                     /locus_tag="PLW15_02185"
     CDS             complement(14573..15394)
                     /locus_tag="PLW15_02185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT33240.1"
                     /translation="MSPAAPVALPGLAGKTVVVTGALGGQGIAETLALLASAADVIAT
                     DLAPGPSTELTDAAAPLPGTLTYRQLDVSSEGGWAALAEFATGLGGGVQGLVNNAGIP
                     FRARLDDIELADWDRVIGINLTGPMLGIRTLSPLMPRGSSIVNVGSLAGLNAHHTVAY
                     TTSKWGLRGLTHVAATELGARGIRVNIVHPGYVETPMMAAAPAIMTEAQLALTPLERT
                     AQPEEVASVVAFLLSDLASYVTGAEIPIDGGFSSSGGVKYLSDTIARNQAPKAGA"
BASE COUNT         2460 a         5254 c         5139 g         2483 t
ORIGIN      
        1 cgagttcgcc gaattcgcgg actcccaggg gctgacgccc cccgtcgcgg ccctggcctg
       61 ggtgtcgcag ctgcccggtg tgacgaccgt catccccggt gcgcgcaatg tgcgccaggc
      121 ggtgtccaac gcgtcggccg gaagcgtgcc gcagttgacc ccggagttca cggagcagat
      181 caccgagctc tacgaccgca cgttccgtga ggccatccac ccgcgatggt gacctcgtcg
      241 cgcgggggac tgggccggcc gttcgccaac ctcttcaccg ccaacctcgc gtccagcctg
      301 ggcgacggaa tcgcgcgaac cgcgatcccg ctgctggctg cgcgactgac gagcgacccc
      361 ttcctggtgt cggtggtcgc cgcgctcgcc ctgctgccgt ggctgttctt cgcgatcccc
      421 gcgggcatcc tcatcgaccg catcgatcgc cgccgcgcgc tcgcgatggc gcaggcggtg
      481 cgcaccgcgc tcgcggtcct gctcatcggc ctggtggcga cggactcgct cacgatctgg
      541 tggctctaca tcgtggtgtt cgtctacggc atgttcgaga ccgtctatga cggagcgatc
      601 cgagccgtcg taccgagcat cgtcgaccgt gcgaacctgc cccgcgccaa ctcgcgcatc
      661 gagggcggcg agctcgtcgt gcagaacttc gtctcgggcc ccgtgacgtc ggccctgttc
      721 gcggtgtccg ttctcgtgcc cctcggtatc aacggcctcg tgttcgcggt ggctggtgcg
      781 ctcgcgctgg cgcttccgcg cgtggcatcc ggtcggcagt tcgtggccca cggcgagact
      841 caggtggcct ggtaccggca gttcgccgac gggctgcggt tcatctgggc caatcgcatg
      901 ctgcgcacgc tctggctgtt cacgaccttc atcggcctcg ccacgtcggc ggccacagcg
      961 accttcgtgc tctacctgct caaccgtctg gggattccgg aggccgcgtt cggcctcttc
     1021 atgctcagcg gtgcggtggg cggcatcatc ggggctgtgc tcgccaaccg cctcaaggat
     1081 gcctggggcg ctggcctcac catggccgtg atgaccacgc tctgcggtgt ggccttcgcg
     1141 ttcatcggac tcgtgccgtc gatctgggcg gccgcggtcg gcttcgccgt gtcatccctg
     1201 gctatcacga tctggaacgt gctcatcatg tcgctgcgcc agagcctcgt gccgggccac
     1261 ctgctcggcc gggtgcacgg cacctggcgc accctgctgt ggggcacgat gccgctcggg
     1321 tcgctcatcg gcggactcat cgctcgcgtc gacctcggcc taccgttcat catcggcggg
     1381 ggagcggcgg cagtggcgag cctgatcttc ttcgccttcc tgcgcaccct gcccaacccc
     1441 gaggacgtcg agctacccca aggacccggg ccgaccgacc cgctcgtcgg cgagtagcca
     1501 cccgatcagg gcggcgatgg tctcctccgg ccgatcgcgg tgcggggagt ggccggcgtc
     1561 ggcgaccacg cggtagtcga cgagcgggtt ggcggcggcg agggcatccg ccgtctcgtc
     1621 agacagcatg gagtacacgg cggggtcgcc gcgcagcagc agagtgggca cggccagcgc
     1681 tgtggcctcg gccgtgacat cccaccgatc gttgtcggtg aagctgtgct cgatcacgtg
     1741 ggcgtccgtc tgccggatgc ccgccagctt ggccgcgcgg tcgcgctcgt gccagtgcgg
     1801 cttcagtgcc ccgagcgcct gttcgtcgag gccgagctcg gagagctgat cgtcgatggt
     1861 ggcctgccac tcgtcccggg tgaccagcag cacggggtcg agcagcgcaa gggcccgggt
     1921 gaacccgggg tgctgcgcgg cgagcaccgc tgccgcgccg cccagggagt ggccgatgac
     1981 ggcgaaccag tcgtcgccgg gcaggtcacc ggcgtagtcg acgagggcat accgctcggc
     2041 acggggcgcc tcgccgtggc cgcgcaggtc gacggcggtg acccggaagc cggcgtcggc
     2101 gatccctggc ccgacgcgcc accaggactc ggccgtggag ctgagcccgt gcacgaggag
     2161 cacgcgcggt ccccgctcgc cccagtgacg ctgcggaagg tcgatcatgc ggccacgata
     2221 gcgcgcggga atcgcggccc ggtcagcgtg gcaggtactt gtcgagggtt gtcaggtatc
     2281 gttcgcgatc gaagtcgggc atcagggcgg cctgcacgat gaagccctgc gctccgctca
     2341 caaacaggga cacctgctcg gcgccgatgg cgtcgccctc ttcctccgag acgccgtgct
     2401 cgcgctggtg ccagagcgag atgtagcgtc ggtacgctcc ctgcagctgc gcgaacacgc
     2461 cctgtgcgag gtccctcaga gcaggatcgg tcgccgcctc gccccacagc tgcacgagga
     2521 ttgtcgggtc gccgaggtct tcgagcatcc ctcccatgag caccctcaga aggtgagagg
     2581 gtggcggcag cgggtcggag gccatgagct gttgcacgtc atcgacccgg gctccgacga
     2641 cccgggtggc gacatccagg atgatgtcgc ccttgctggc gaagtggccg tagatcgcgc
     2701 cggccgatag ccccgattcg gcgatgatgt cggccatgga tgcgccctgg aaacccttgc
     2761 gccggaaggc ccgcagggcg gcgtcggcga tctcctcgcg cttggcgatg cggtactcgt
     2821 cggtgacctt gggcacgggt ccaccctaaa aacgaatgaa cgttcttgac aactcgactc
     2881 gccggtgcaa ccctaaaaga acgaacattc cttttgagag gcttcccatg tcgactcccg
     2941 cactcgtcgc ccaccctcac actcccttcc gtcgcgtcgt cgtcgtcggc gtggcgcttg
     3001 cagcagtact gagcgtcatc ctgctggcct tcctctggcc cactgtgacg tcatccgtca
     3061 aagccctgcc gatcgccatt gccgcgcccg ctgagcaggc cgaccagttg gctggcgcgc
     3121 tggaggagag gtcaccggga gcgttcgacg tgacgacggt ggcggatcgg gccaccgcgg
     3181 tcgacctcat cgaaacgcgt caggcctacg gagcgatcgt gctcggctcc gaccccgagg
     3241 tgatgacggc gtcggcggcg agtccgctgg tggctcagct gctcaccggc ttcgcgcccg
     3301 ctctcgcggc gcagctcaac gccgcggccg ccgctcaggg catcgccctc cccgcaccac
     3361 tcatcgtgac ggtgaccgac gtcgtgccgc tggcatcgac cgacgctcgt ggtctcggtc
     3421 ttgcatcatc gagcttcccc ctcgtgctcg gcggcatgat cggcggcatc gcggtctcgc
     3481 tcgccatcgt cggggtgtgg cgtcgggtca cggcaaccct gatctactcg gcggtgggcg
     3541 gcctcgcgat cacgggcatc ctgcagggct ggttcggcgc tctccagggc gactacttcg
     3601 tcaatgccgc ggcggtagcg ctgacgattc tcgggatagc gggaacgatc gtcgggtgcg
     3661 tctccctctt cggtcgcccc ggcatcgccg tcgggcccgt gctgttcctg ttcatcgcca
     3721 acccgatctc ggccgccgcg cagccggtcg agttcctcac ggcgccgtgg ggagccatcg
     3781 gtcagtggtt cccgccgggg gccgccgcga ccctcctgcg ggagctcagc tacttcccgc
     3841 gagcggacac gctgttcccg tggctcgttc tcgcggggtg ggcggtgctt ggactgctgc
     3901 tgtcgaccct cgggcactac cgcgaccgtg gcgccgcgac gcaggcagct ctcgccgccg
     3961 ccgagtagcg cggcgacaga agcgcggccc ggggcgcagc gaccctcgcc gcaccctcgc
     4021 ggaatagcgc ccacccgccg gatggttgac ttggcacatg acgaaactga tgatcatcct
     4081 cggcagtgtg cgaccgaagc gggccggcga ggccgtcgcc gagtgggtgc ggggtgccgt
     4141 ggccgcgcac caccactcct tcgaggtgga cttcgtcgac ctgcgcgaac tcgccctgcc
     4201 gttcatggat gagccggagc atccggcaaa gaaggcgtac acgcaaccgc acacgatcgc
     4261 gtggagccag cgggtggatg ccgcagacgc cttcatcctc gtgagccccg agtacaacca
     4321 cagctactcg cccgcgctca agaacgccct cgacttcctg gccaccgagt ggaagcataa
     4381 gccggtcggt ctcgtcagct acggcggagt ctccggcggc acccgcggcg tcgccgccct
     4441 cgatcccgta ctcgcgacgg tgggcctcgt gaagacctcg cacagcgtcg agatcgacta
     4501 cgtgagtacc cagatcgttg acggcgtctt caccccggcg gagaagcacg cagccctgct
     4561 cagccaccag ctcgacgacc tcacagagct ctccgccgtg cttgcgacgc tgcgctaggc
     4621 accgcctcct agggcactat tgcttctgag aggggatgac gatgagcccg accaagcgca
     4681 acgtcacggc gaagtggccg gggttcacca ccgacctcct gcgcggtgac ggagagcacg
     4741 ccgaccgggt gagcttcctc gagctgttct tcgacctcgt cttcgtcttc gccgtcaccc
     4801 agatctcgca cacgctggtg cactcgatcg acgatggaca cgcgctcgac ggcgggcttc
     4861 aggcgacggt gataatcctc gccgtctggt gggtctgggt gtacaccgcc tgggtgacga
     4921 actggctcga ccccgacaag gcggccgtgc gctggatgct catcatcctc atggtgcttg
     4981 gcctgctgat gagcacctcg atccccgagg ccttcggcga ccgcgcgctg ctgttcgtct
     5041 ccgcctacgt cgccatgcag gtcgggcgtg cgatcttcac gatcctcagc atggtgcggt
     5101 tcgtaccgga gaacgcgctc aacgtcaccc ggctgaccat ctggttctgc gtcagcgcga
     5161 tcttctggtt ctggggtgca ctcaccgaag acctgacggc gcgactggtg ctctggggtg
     5221 tggcgatcgt gatcgagtat ctcggtccgg tgtcccgcta ctgggttccg gggcttcgca
     5281 gctcgtacat cggcggcatc cccatccgcg gcggtcacat gtcggagcgg gccgggctct
     5341 tcgtgatcat cgccatcggc gagtcggtgc tggtcacggg aacggccttc gccgaccatc
     5401 cgatcgacct gcccaacacc ctcgcgttct tcgcgtcgat caccgggagc atcctgctgt
     5461 ggctgctgta cttcagcagg gccgagtcgg gcggctcacg cttcatccgt cgccacagca
     5521 atcccagcct ggtggccgcc aacagctaca cctacctgca cgtcgtgctc gtcgcgggaa
     5581 tcgtcgtcat cgccgtcgcc gacgagctgg tgctcgcgca cccgatcgat gcgcccaggc
     5641 agctgaccct ggcactcgtg tacggcgcgc ccgcgctgta cctcatcggc aacctggtgt
     5701 tcaagaagtc catcggcgcc ccgtggctca agtcgcacct tgccggtgtc ggtattctcg
     5761 tgatcttcgg tgccgttgtg ctgctgtcgg gccttcagct gcccgctctg gtgcactcct
     5821 ggatcgccaa cgttgtgctc atcgccgtag tgatcggcga ggaaatcgac ttcagacgcg
     5881 aacgaagccg ccttcgttcg ctacggtgag tgccatgacc gacgccgtca cccccgcgcc
     5941 cgatcccgcc gcgacctccg caacgccacg cccgcgccgc acttgggacc tcatcctcac
     6001 gatcgttctc ctgatcgcct accttctcgg cacgctcgtg gccagcttca gcgccttctt
     6061 cctcgccttc gcgggggaca gttgcggtgc atcgagcgtg tgcgatgaga accagatggc
     6121 caacggcatg atgatcgccc tgtacgcggt ctggatcccg gcgctcttcg tggtcatcgg
     6181 cgcgatcgtg ctgctcgtgc tgcgccgact cgcgttctgg gtgccgctgg tgggtggcgc
     6241 gctcacgatc atcatctcga tcatcggatg ggcggtcacg atctccgcag tccgcaccgg
     6301 ctagggcgcc atccgcggtc gtcggcctga gctcgtcagc tgggctggat cggtaccggc
     6361 tccgtgtcgg ggatggggct cgcgtcccga ccccgcagat ccggatgcgc ccggcggaac
     6421 accagaggaa cgatcgcgcc gaccgcggcg aaccctgcgg ccgtccagaa cgcaccaccc
     6481 gcgccggccg agtcgatgag gaagcccgcg accgctgacc cgatcgccgc gccgatgagc
     6541 tgtccggtgc ccacccagcc atacgcctcg gcggtgtcgc tgaacttgac gcttgccgag
     6601 acgatcgcga acatcaccgc gagtgctggc gcgatgcaga gtccggcaac caacagggcg
     6661 gcggacagcg accagaagtc ccaggcgaag atcgcgaggg cgcagccgac ggcgaccacg
     6721 atcatccgcc tggccagggc ccacggcccg accgggatgt gtccgagcag cagaccgccc
     6781 gcaagcgacg agatggccca cagcgcgagc accgttccgg cgaccggccc accctcaccg
     6841 aaggtggcga ccacgcccgc ctcgacggcg gcgcaggatc cgacgagcag gaagcccacg
     6901 acggtggcga gcagcacggg cggcctcgcc agcacgactc ccagtcgacg cttcgagcgg
     6961 gggatgcgca cccgaccgac ctcggggctg gagatgaacc acgccccgcc gaagatgagg
     7021 aaggccatgg ccatgacgat gccgagcacg gtggagatct ggatggagac gaaagtcacg
     7081 agcacgggac cggcgatcca gatgatctcc tgagccgagg catccagcga gaacagcggg
     7141 gtcagctgct gcgaattgac catcttcggg tagatggtgc gcaccgcggg ctggatgggc
     7201 ggatagctca ggccgccgat gagtccgacg atcatgtagc cgaccagggg cagcggcacg
     7261 aaggcgatgg tggccatcga gagggcgcag atcgaggtgg tgaggatgag cacggggcgg
     7321 atcgcccaga ggcccatcca gcggctggtg accggtccgg agaccgcctg accgacgctg
     7381 gtcgctgcca gcacgagccc cgcagcgccg tacgagttga agatgtgctc gatgtgcagc
     7441 aggtaggcca gtgagagcat cccgccggga aaacgcgcga ggagctgcgc cgacaggatg
     7501 cgcgcaactc cgggggtttt caacaggtcg atgtagctct tcacaagatc accagcttag
     7561 ccccgggact taccgctggt ttcggggcgg tatcggtgtc ggaggcgcct cgtagcctgt
     7621 ggacaagtcg tgcaagttat gcacatctgt ggacgacacg cccactatat gtagggttcg
     7681 catcgtgtcg gtacccggat atatagtgat cagaccgtca ggccggggga atccgccgga
     7741 tggggaattc ggggatgaga tgccccacgg tgagggcaat ttcagggttg caagagggag
     7801 atttcgtgtc aattacagtc gtcaagcgca gcggtcagcg ggagccctac gacgccaaca
     7861 agatcaacct cgccatcgaa gccgcaaccg tcggtctcga cgacaacatc acctgggtga
     7921 cccagatcgc ctcggagctc gagctcaccc tcttcgacgg catcaccacc cagcagctcg
     7981 acgaggccgt cattcaggtg gcgctgcaga acgtcaagga tgacccggcg ttcgacgtcg
     8041 tcgccgcccg gctgctgctc aagaccatct acaagcgcgt gctcggcgac tacgagacgg
     8101 ccgaggagct caaggagctg caccgctcgc acttcgcgcc caacgtcgcc cgcggcgtcg
     8161 aggagggtct gctcgacccg cgcctgaccg agatgttcga cctcgacgag ctcgcgtccc
     8221 acctcgagcc cgcccacgat gagctgctca agtacatcgg cgtcgtgacc ctcaacaacc
     8281 gctacggcat caagggtcgc aacggcgacg cgctcgaggt tccgcagtac ttctggatgc
     8341 gcatcgcgat gggcctctcc ctcaacgagg cgaaccccac cgagaccgca ctgaagttct
     8401 accgcaagat ggccagcctc gagtacctgg ccgcgggatc aactctcgtc aacgccggaa
     8461 ccatctaccc ccagctcgcg aactgcttcg tcatggagat gcaggacgac atggagcaca
     8521 tcgccaagac cacgcgcgat gtgatgtggc tcaccaaggg caccggcggc atcggcctct
     8581 cggtgaccaa gctgcgcgcc cagggctcgc ccatccgctc gaacaacacg acctcgaccg
     8641 gcccgatccc gttcatgcac acgatcgact cgatcctgcg ggcggtcagc cgcggcggca
     8701 agaagttcgg cgccctgtgc ttctacatgg agaactggca cctcgacttc cccgagttcc
     8761 tcgacctgcg tcagaactcc ggcgacccgt accgccgcac ccgcaccgcc aacaccgcgg
     8821 tgtggatcag cgacgagttc atgaagcgcg tgcagaacga cgacgactgg tacctcttcg
     8881 acccgctcga ggtctccgac ctcaacgagc tctacggcaa ggccttctcc gagcgctaca
     8941 acgagtacgt cgccaaggcc gaagcgggcg agatgcgcct gttcaagaag atcaccgctc
     9001 gtgagcagtt caaggcgatc ctcatctccc tccagaccac gagccacccg tggctcacct
     9061 ggaaggacac gatcaacaac cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnttcggaaa gatcgaggag tcggcccgcc tcgcggtgcg ccagctcgac aacctcatcg
     9241 acatcacgcg ctccagcgtc aaggaggcgg acttctccaa ccagcagaac cgcgccgtgg
     9301 gcctcggagt catgggcttc accgatgtga tcgagaagct cggcatcagc tacgagagcg
     9361 aggaggccta cgacctcatc gacgagatcc tcgagcacgt ctccacgcgg cgatcgacgc
     9421 ctcggctgag cttgccaagg agcgtggcgc gtacgccaac ttcgagggca gccgctggtc
     9481 gcagggcatg gtgccgctcg actcgatcgc cctcaccgag gccgatcgcg ggttggccat
     9541 caaggtcaac cgcacgaccc gactcgattg ggacgccctg cgcgcgaagg tcaagggcgg
     9601 catgcgcaac gcgacgctca tggcgatcgc gcccacggca tccattggtc tcgtcgccgg
     9661 caccacgccg ggtctcgacc cgcagttcgc gcagatcttc agccgcgcca cctcgaacgg
     9721 caagttcctc gaggtcaacg ccaacctggt gcgcgacctg cagaagcgcg gtctgtggga
     9781 gtcgacccgc gaggcgatcc tgcgcagcca gggcgacatc cagtcgatcg cgtccatccc
     9841 cgacgagctc aaggcggtgt acaagacgag cttccagctc tcgccgtacg ccttcctcga
     9901 ggtcgctgcc cgggcccaga agtggatcga ccaggcgatc agccgcaaca tgtacctcga
     9961 gacgcgtgac ctcggcgaca tgatggacat ctactacgcg gcgtgggagc ggggcgtgaa
    10021 gacgacctac tacctgcaca tgaagccgcg ccacacggcc gagcagtcga ccgtcaaggt
    10081 gaacaaggcc gaggagatcg tggcatccgt cgccgccccg gccgctgcgg atgctccggc
    10141 cgccgcagcc cccgcccgcc gcgggttcgg cgggctcgcc ccgaaggccg gtgcgtgatg
    10201 gatcgcgcca ccgccgtcga ggagtactac gtgccatcgg acccgatgga cgacctgcaa
    10261 tgtgaaagct gccagtaatc atgggaatcc taggaacagg ccttcaggac ggcctgctgc
    10321 tcaagccgat ccgctaccag tgggcgatgg acctgtacga ccaggcggtc gcgaacacgt
    10381 ggttccccaa cgagatccag ctcggtgaag acatcgccga cttcaagaag atgaccgacg
    10441 aggagcgtca cgcggtcacc ttcctgatga gctacttcaa ccccaacgag ctgctcgtca
    10501 acaaggctct ggcattcggt gtctacccct acgtcaacgc ggccgaggca acgctgtacc
    10561 tcgcgaagca gatgtgggag gaagccaacc actgcatgag cttcgagtac gtgctcgaga
    10621 ccttcccgat cgatcgtgag gccgcctacg gctcgcacgt gaacgtgccc tcgatggctc
    10681 gcaaggagga gttcgaggtc aagtacatcc gtcgcatgac ggagcagaca ctcgacatca
    10741 ccacgaccga gggcaagaag gacttcgtgc gcaacctcgt cgcgtacaac gtgatcctcg
    10801 agggcgtctg gttctactcg ggcttcatgg tcgcgctgag cttccgccag cgcaacctgc
    10861 tgcgcaactt cggctcgctc atggactggg tggtgcgcga cgagagcctg cacctcaagt
    10921 tcggcatcaa cctgatcctc acggtgctcg aggagaaccc cgacctgcag acggaggagt
    10981 tcgccaccga gatcaagcag atgatcctgg atgccgtcga gatggaggag gcgtacaacc
    11041 acgacctgct tcccaacggc atcctcggcc tcaacgcgac gtacatcaac cagtacgtca
    11101 agtacctggc cgaccgtcgg cttgaggagc tcggattcga ggcgcactac aacgtctcca
    11161 acccggccaa gtggatggcg gccgccaacg acaccctcca gctcgtcaac ttcttcgagt
    11221 cgaccaacac gtcgtacgag tcgaacgcgt cggccacggt gggcgccaag agcgagtgat
    11281 taggcggcgg ccggttgagt agccgcgatg cggcgtatcg aaacccgatc cgcatcgagt
    11341 acgtcgactg gctcgatccg cgcgcggtcg ccctgcgcga ggcgatggat gccgagacga
    11401 gcgccatgta cgcggcgttc gcgtctggcc agagtgcgga agtgacggca gccatcgccg
    11461 acgcgctcac cgtggatccg gcagagatcg tcaccacgat cgtcgcggtc gacggcgacg
    11521 agccggtcgg gcactcggcg ctgcggccgg tacatctcga cacgctcgat gaccgggctg
    11581 cgcccgatga ccgggctgcg cccgatgacc gggtgctcga ggtgaagaag gtgttcgtcg
    11641 ccgagagcgt gcgtggtcgg ggggtcgcac ggctgctgat gggcgaactg gagaccgtcg
    11701 cgcgcggcag gggcgcggca tccctcgtgc tgcagaccgg ccccctgcag gtgccggcga
    11761 tcacgctgta cgaagacctc ggctacgtgg cgatcccgcc ctacggcaag tacgacgcga
    11821 ttcccggggc gctgtgcttc gagaaggtgc tgtagctact tcggcgccat gcggatcgcg
    11881 ccgtcaagac ggatcgtctc gccgttgagc atcgcgttgt cgacgatgtg cagggcgagt
    11941 gcggcgtact cgtcgggtcg tcccagccgt gacggatgcg gaacctgcgc cgcgagggag
    12001 tcctgcgccg cctgcggcag gcccgacatc atcggcgtct ggaagatccc cggagcgatg
    12061 gagacgaccc gtatctgggt gtgggcgaac tcgcgggcca gcggcagggt catcccggcg
    12121 acccccgcct tcgacgccgc gtacgcgggc tggccgatct ggccatcgaa cgccgcgacc
    12181 gacgcggtca tgacgatcac cccacgctcg ccgtcgatcg gctcggtcgc catcatcgct
    12241 gctgcggcca acttcacggt ggtgaaggtg ccggtgaggt tgatggcgat cacgcgctcg
    12301 aatcgctcca gcggcagcgg cccgtcgcgc cccacagcac gctcggcggt ggcgactccg
    12361 gcgcagcaca ctgccacgcg cagggggccg agctcggtcg cggcctcgac cgctcgagcc
    12421 accgagtgct cgtcggtcac gtcggcggcg acgaaggtga ggccgtgctc ggccgccagc
    12481 tcggtgccgc gcgaggaggg gaggtcggcg atgacgacgt gcgcaccgcg ccctgcgagg
    12541 gcgagggcgg tggcgagtcc gagcccgctc gcgccgccgg tgacgagagc cgagcatccg
    12601 gcgatcttca tctagagcct ctcgatgatg gtggcgttgg ccatgccggc accctcgcac
    12661 atgacctgca ggccgtagcg accccccgag agttcgagct ggttgaccat gctggtgagg
    12721 atgcgcgttc ccgacgaccc gagcgcgtgc ccgagggcga tggcgccgcc acgggggttc
    12781 agccggttgt ggtcgatctc gaactcgtgc tgccaggcca ggggcaccga ggcgaatgct
    12841 tcgttgatct cgacggtgtc gaagtcgtcg atggagaagc cgctgcggtc gagaatcttg
    12901 tgggtcgccg ggaggattcc cgtgagcatc tcgatcgggt cgctgccgac cacgaccgac
    12961 gagtggaagc gcgcgcgggg gcgcaggccg aggtggttcg ccttctcctc gctcatgatg
    13021 agcacggccg aagcgccgtc ggtgagcggc gatgagttgc cggcggtgat gcgccagtcg
    13081 atctcgggga atcgctctcc cagcgcccca tcttcgaagg cgggcttcag actcgcgagc
    13141 ccctccgccg tcgtgcccgg ccgaatggtc tcgtcggcct cgaagtcgcc gacgggcatg
    13201 atctccgatt cgaaggatgc cgcggccgcg agccggtgcg accgtgcggc gaactcgtcc
    13261 agcgccgccc ggctcaggtt ccagcgagcc gcgatcaact ccgccgagat gccctgcccg
    13321 accagaccgt cggggaaacg ggaccgcacc atgggtcccc agttgtcgag cccgagcgtt
    13381 gccgagttga tggggttctt gctcatcagt tcgaccccgc acgcgatcac cacgtcgtac
    13441 gcaccggcaa tcacgccctg cgcggcgaag gcggccgcct gctggctcga cccgcactgc
    13501 cggtcgatcg tcgtgcccgg caccgagaac gggaagccgg cggcgagagc cgcattgcgg
    13561 gcgatgttga ccgactgctc gccgatctga gagaggcacc cggcgatcac gtcgtcgatg
    13621 agcgcggggt cgaggtcgtt gcgcgcgacg agcgtcgcga gcacggtgcc gaggaggtcg
    13681 accgggtgct caccagacag cgcgccgccc ggcttgcccc ggcccgaggg ggtgcgcagg
    13741 gcatccacga tcacagccgt tgacatgctc tcaggctatg cccgttggcg ggcgggggcg
    13801 aggggtcagc ccggcaacac ggccgaccag ccgccgtcga tcaccaggtt cgtcccggtg
    13861 atgtacgacg cctcgtcgct ggcgaggaac agcgcagccc tggcgacctc gatcggctgc
    13921 ccgatgcggc cgagtgggat gtgctgtgcg atctggcgca tggggtggtc gtcggcgagc
    13981 aggtcaccct gtgtcgccgg cgtgaggatc atgcccgggc tcacacagtt ggcacggatg
    14041 ccgcggctcg ccccctcggc ggcgagctgc ttggtcatcg ccaccacgcc gcccttggtg
    14101 gcggtatggg cgatgcgcgt gttggtcatc gacccggtga ttcccgcggt ggacccgacg
    14161 agcacgatgc tcgcgccctc cgacaggtgc ggccaggcgt ggtgcaccgg caggaacacg
    14221 atgtcgagct cgtggcgcag cacccacgcc cagtcgtcgt agctcgtgtc ggcgaccggc
    14281 gcgaagcggg tcgccccggc gttggcgtag aggatgtcga tgcggccgaa gtcggcgacg
    14341 acctgggcga tccagtcgcg aactgctgcg tcgtcggtga gatccagcgg gtgggtgctc
    14401 gtgatggtgc cgccagccgc ggtgaccagc cggacggtct cggcgctgcc cgcggcatcc
    14461 acgtcgcagc cgacgacgat cgcgccctcg ctcgcgaaca gctcggcggc ggcccgcccc
    14521 tgtccgctcg cggtgcctgt gatgacggcg accttgccgt cgaggcggcc ggtcatgcgc
    14581 cggccttcgg cgcctggttg cgggcgatgg tgtcggagag gtacttgacc ccgcccgatg
    14641 acgagaagcc gccgtcgatc gggatctccg cccccgtgac gtagctcgcc agatcggaga
    14701 ggaggaacgc gaccaccgac gcgacctcct cgggctgggc ggtgcgttcg agcggggtga
    14761 gggccagctg cgcctcggtc atgatcgcgg gagccgcggc catcatcggg gtctccacgt
    14821 acccggggtg cacgatattc acccggatgc ctcgtgcccc gagttcggtc gctgccacgt
    14881 gggtgagccc gcgcaacccc cacttgctgg tcgtgtaggc cacggtgtgg tgggcgttca
    14941 gcccggcgag cgagccgacg ttgacgatgg atgagccgcg cggcatgagc ggcgacaggg
    15001 tgcggatgcc cagcatcggc cccgtgaggt tgatgccgat cacgcggtcc cagtcggcaa
    15061 gctcgatgtc gtccagcctg gcacggaacg ggatgccggc gttgttgacc agtccctgca
    15121 ccccaccgcc gagccccgtc gcgaactcgg ccagcgcggc ccagccaccc tcggagctga
    15181 cgtcgagctg ccggtaggtg agggtgccgg ggaggggagc tgcggcatcc gtcagctcgg
    15241 tcgacggacc gggggccagg tcagtcgcga tgacgtcggc ggccgacgcg agcagcgcga
    15301 gagtctccgc gatcccctga ccgccgagcg cccctgtgac cacgacggtc tttccggcga
    15361 ggccggggag ggcgaccggc gcggcaggac tcatgcgggc tcgaccccga aggcgcgcgc
    15421 gagcttgtcg atcttc
//
LOCUS       DAOSOU010000022        15034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_3991_length_15034_cov_6.349222, whole genome shotgun sequence.
ACCESSION   DAOSOU010000022 DAOSOU010000000
VERSION     DAOSOU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 15034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15034
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3991_length_15034_cov_6.349222"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            522..2270
                     /locus_tag="PLW15_02190"
     CDS             522..2270
                     /locus_tag="PLW15_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HOT33241.1"
                     /translation="MTHRQILFVIFGLMAGMFLSSLDQTIVGTSMRTIADDLDGLSLQ
                     AWVTTAYLITSTIATPIYGKLSDIFGRRPLFIIAIVIFLIGSLAAGFSTSMYELAVFR
                     ALQGLGAGGLMALPLAIMGDILAPRERAKYQGYFLAVFGVSSLIGPLVGGLFAGADQI
                     LFITGWRWVFLVNLPVGIIALAMVLRFLHIPHTRRKVRIDWWGAATVVIALVPLLLVA
                     EKGRDWGWDSFYAITCYIVGGLGIAAFIISEKLMGDDALIPLKLFKHPTFSMSTVLGV
                     LVGFGMFGAMMTIPLYLQLVNGATPTESGLLSLPMILGLMISSIASGQIIARTGRYRV
                     FPTLGTAFMAVGFFWLTFLTADKPLIWIMVGMLLIGLGLGQLMQTLTIAAQNSVGPRD
                     IGVATSSSTFFRQIGGTLGTAILFSVLFTRIPQTIADAFASTSVKDGIAAAVADPKVL
                     ADPANKQILELFQQAQNGGSVGNALDGDTSFLNGSDPRLAAPFLEGFNNAAVSVYWVA
                     LAVVLIAFVLSFFLKAAPLRAKSALQENADADAAVIAQEAADLAGAMVAPDLDTTHEA
                     SEPESAPTGKRPKRTT"
     gene            2518..3774
                     /locus_tag="PLW15_02195"
     CDS             2518..3774
                     /locus_tag="PLW15_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33242.1"
                     /translation="MRTSRRTIAATIAGAATLALVVTGCSAGGGDSSDEPVTLTLATF
                     NDFGYTDELLAEFTAETGITVIHNKAATSNDARANYFQKLGNSGLADVEAVEIDWFTE
                     AMQYSDLLAPVADDLKGRWLDWKEAAATDAAGNLIAYGTDIGPQGICYRSDLFAAAGL
                     PTDRAEVAKLFDGDWDNFFSIGAQYTATTGKPFIDSANSVLQGIVNQYELAYEDTDGS
                     IIATTNPDIEAAYKAVVEKAIPISAYSGQWSEDWFASMASGEFAAMLCPGWMLGVISG
                     NAPDITGWDIADAFPGGGGNWGGSYLTVPANGAHVKEAAELAAWLTSPETQLKAFTNA
                     GTFPSQVDALASDELAGATNEYFNNAPTGTILTDRANAITVATFKGPKYFQFHDALQN
                     AVTRVFDGLEDEQTSWNNWVTEVSAF"
     gene            3855..4850
                     /locus_tag="PLW15_02200"
     CDS             3855..4850
                     /locus_tag="PLW15_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT33243.1"
                     /translation="MSTATAKSPKQVRVVSFSHRMSRWDLKLSPYLYISPFFLMFLVV
                     GLFPIIFTAVISFQDWDLVRGSGKFVGFDQYIWILQQPHFWTALRNTFSIFLLSTIPQ
                     LLLAIFIAAMLDRNIRAKTFWRMGVLLPFVVAPVAVGLIFNVVFADNFGLVNGFLTDI
                     GLPAIQWHKEPFWSHVAIATMVNYRWTGYNTLILLASMQAVNREYYEAATVDGAGRFR
                     QFLYITLPSLRPTLIFVVITSTIGGLQIFDEPRVFDQFGRGGAGQQWLTITLYLYDIG
                     WGQWNFGRAAAMAWILFVIILLIGLLNLLITRTLVRDEGTRVVKLSRRERKELAR"
     gene            4847..5752
                     /locus_tag="PLW15_02205"
     CDS             4847..5752
                     /locus_tag="PLW15_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT33244.1"
                     /translation="MSTTPPIAIVEESIPNERQRRRGERTTRIRGNRPGFVVYAVLAV
                     VFISAAFPLWWSFVIGSGDASSLRERPWWPGGNFLANALAVVSNPAVNFWAALWNSIY
                     SSFLIAASVVITSTLAGWAFAKLRFLGGPALLAFVVATMAVPQQLGVVPLYILFSDLG
                     WTGSIGAIIMPALTSAFGVFWMTQYLRQAVPNELIEAARVDGATMIRTFWTVGVPAAR
                     PAAAMLFLFIFVGAWNNFFWPFIVLDRQNPTLPVALSLLQSNYFVDYSIVLAGVVLAT
                     VPLLLLFIFAGRQLVSGIMAGAVKG"
     gene            5792..7219
                     /locus_tag="PLW15_02210"
     CDS             5792..7219
                     /locus_tag="PLW15_02210"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOT33245.1"
                     /translation="MSADAHRTFPPTFLFGAATAAYQIEGAAHEDGRTDSIWDAFCRV
                     PGAVVGGDNGDVACDHYHRYRDDVALMAELGLQTYRFSTSWSRVRPDGGAVNAQGLDF
                     YSRLVDELLAQGIMPWLTLYHWDLPQALEEKGGWTNRDTSYLFAEYAASVHDALGDRV
                     TAWTTLNEPWCASFLSYIGGEHAPGRQDPAAGLAASHHLLLAHGLAVQELRSRDSSLQ
                     LGITLNLTVADPVDPSDEGDLDAARRIDGQFNRWFLDPIFRGSYPEDVLQDVAGLGFE
                     SVVQPGDLEIISSPIDALGVNYYHGEAVSSKPAEHEITTQAPSARPKRSPFPAADSVH
                     WHRRDLPVTAMDWEVQPEGLTRLLIRVHEEYTSGAGVSLAVTENGAAYTDVVAADGAI
                     HDADRVTFLQWHLAAILDAIDAGVPVSGYFYWSLMDNFEWAWGYDKRFGIVRVDYDTQ
                     ARTPKDSAIAYTRIITGRALPDSAG"
     gene            7229..8254
                     /locus_tag="PLW15_02215"
     CDS             7229..8254
                     /locus_tag="PLW15_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT33246.1"
                     /translation="MTLHSPARRVAPTLEAVAAEAGVSRSTVSRVVNGSEHVRPDVVT
                     AVTAAIERLNYVPNRAARSLANRQTMAIALVVPEDTTRFFGDPFFSEIVQGITQGLED
                     SDYVLNLQLASPSAPSEKTIRYLLGGNVDGAIVVSHHSGDDFFTTLDATIPVVFGGRP
                     FHPEAHQNSFVDVDNRAGGALGTQYLIDLGRRRIATIAGPENMQAPIDRHQGWLDAVT
                     AAGLPTDLVVHGDFTLASGAAGMRELLDRSGEIDGLFVASDLMATGAISVLRERGIRV
                     PEDVAVVGFDNSSAATSGSIGITTVHQPSREMGAEMARMMLAMLRGERPERERVMPTR
                     LVVRDSA"
     gene            8280..8564
                     /locus_tag="PLW15_02220"
     CDS             8280..8564
                     /locus_tag="PLW15_02220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33247.1"
                     /translation="MASEVIQDVSMNRFELLVDGEQVGLSDYRIRDDSIVILHTEIDP
                     SRRGEGLGDLLARGMLNLIRADTDHRVVPLCPFMKKWIDEHKDYQDLLTR"
     gene            complement(8604..8894)
                     /locus_tag="PLW15_02225"
     CDS             complement(8604..8894)
                     /locus_tag="PLW15_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33248.1"
                     /translation="MDWLIFLGIAAVLIALGFVAQHYGWIDLSNKSRTSGKAGGGVMG
                     IGDEVFNPSRYESQLELDRQTMLPAPAPLPGDGDKDIYKGNVKIDLKPRDDA"
     gene            complement(8904..9686)
                     /locus_tag="PLW15_02230"
     CDS             complement(8904..9686)
                     /locus_tag="PLW15_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOT33249.1"
                     /translation="MSPYSSRVITAAEVGVDLDGVTVLDGVSLEVIPGEVLCLVGPNG
                     AGKSTLLAVLSGERRPTRGTVSIGGRELGGIRHTELARLRSVLTQENSVSFPFRVSEV
                     VAMGRAPWARTLEGRDDVRAVTEAMDATDIRHLATRRYTALSGGERARVSLARVLAQR
                     AGTVFLDEPTASLDLRHQEDVMRIARGLAAEGRAVVVVVHDLSLASAYADRLALVASG
                     GLEAIGSPAEVLTEERIERVYGLPVELHLVGGRPVVVPTRGT"
     gene            complement(9683..>10200)
                     /locus_tag="PLW15_02235"
     CDS             complement(9683..>10200)
                     /locus_tag="PLW15_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT33250.1"
                     /translation="MLFVADSGSREQIVFWQLGSLNGSRWSEVVIVAVVGVVGTVIAV
                     LLGKRYDLLALGERNARHLGVDVEHLRVGSIVLVALLTGVAVAFVGIIAFVGLVVPHV
                     IRMAIGPSHRPLIVASAFGGGALLVFADLLARSVVPGADLPIGLLTSLVGGPFFFFLL
                     FRQRRQSGGWA"
     assembly_gap    10201..10300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10301..10806)
                     /locus_tag="PLW15_02240"
     CDS             complement(<10301..10806)
                     /locus_tag="PLW15_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOT33251.1"
                     /translation="MRTRAVVLSTSLGVLLVVGVVASAMLGQLAVSPAEVVGSVLRAV
                     GIPNSWAPTDPIVESTLWVVRFPRIAMALTVGAALAVAGAVMQAIFGNPLAEPGVVGV
                     SSGSALGAAIAIVLGVTAFGTGSLAIFAFLGGLGATLLVYFVSRANGRTEVVTLLLTG
                     IAINAFAGA"
     gene            complement(10803..11852)
                     /locus_tag="PLW15_02245"
     CDS             complement(10803..11852)
                     /locus_tag="PLW15_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOT33252.1"
                     /translation="MHGEFKVPGGKLVVVDLETTDGVIHDFRLAGDFFLEPDSALAAI
                     NGAVNGLPADADAARIAKAIQAGLPDDASLLGFTPDAVATAIRRALASATGWRDYDWQ
                     LIMGPALDPVVQMALDQVLAEEVGAGRRKPTLRIWEWEKPAVVIGSFQSVKNEVDLDN
                     AKKYGFDIVRRISGGGAMFMEAGSVITYSIYAPTELVQGMSFADSYAFLDEWVIIALK
                     ALGIEASYVPLNDIASPTGKIGGAAQKRLGSGAVLHHVTMSYDMDGDKMVEVLRIGRE
                     KMSDKGTKSANKRVDPLRTQTGLQRAEIIEKMVGTFRGLYGLTPSEITPEELAQAQQL
                     VTEKFGTEEWLQRVP"
     gene            complement(11943..12371)
                     /locus_tag="PLW15_02250"
     CDS             complement(11943..12371)
                     /locus_tag="PLW15_02250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOT33253.1"
                     /translation="MTSVFTEILRGNSPGRFIWRDDTCFAILTIEPRKPGHTLVMPLE
                     EVDNWLDLSEEVAQHIFRVGRLIGLAQREEWGSPRVGVMYEGYMVPHSHLHVWPSWTV
                     FEYSHTGIQRNAEPADLDAAALRLRARLVANGHGEFVPAD"
     gene            complement(12368..13225)
                     /locus_tag="PLW15_02255"
     CDS             complement(12368..13225)
                     /locus_tag="PLW15_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33254.1"
                     /translation="MPKTTVERTDHSYVDAHGVTIHYYVWKAPKPKAVVQLAHGLGDH
                     ALRYDHVAQALAQAGYSVYADDHRGHGETGITQHAGDKSRLGRLGPGGLRATIAALQQ
                     FTTIIRAENPGLPLALLGHSWGSLMAQTIVNTDAEDYEAVVLTGTAYRTLGDMNGGDL
                     NKGYSTPNEWLSRDPSVAKAFADDPKTFYADTIKLFGLGDALRLLGKPAKKLGRDIPI
                     LIMIGSADSLGGEKSVAKLASDYVTRSGLSDVEAIVYPGARHEIFNETNRDEVIADLV
                     AWLDARLTA"
     gene            13313..13729
                     /locus_tag="PLW15_02260"
     CDS             13313..13729
                     /locus_tag="PLW15_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT33255.1"
                     /translation="MTDDLDDELRIAIMRLARRMRLERADEDVTDGQLSVLFVLTKHG
                     AQTLGALSEHERVTPPSMNRTVNALENNGLIVRESSPDDGRKVLITLTDRARALVAET
                     QRRRVAWFSRQLASLSAAERGALDAAAPILRTLADS"
     gene            13726..15027
                     /locus_tag="PLW15_02265"
     CDS             13726..15027
                     /locus_tag="PLW15_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33256.1"
                     /translation="MSAMFRSLRVFNYRLWFAGALVSNTGAWMQRTAQDWIVLTELTD
                     RDAAALGITMALQFGPLLLLMPVAGLMADRFDRRRLLMWTQGIQCALALGLGLLVVSG
                     HAQLWQVYVFALLLGIATAIDAPARQAFVSELVTDADLSNAVALNSTSFQSARLVGPA
                     VAGVLIAWIGSGPVFLINALSFAGVLASLAFIRRKELVASPRLIRARGQIRDGLHYVR
                     TRGDLVVTLVAVFLVGTFGFNFPIFISTMSTVEFGKGATEFGLLSSIMAAGAVTGSLL
                     AARRTRARLVVAVAASAAFGLACVLGAIAPTYWLFAATLVLIGVASLTMMTTANAYVQ
                     TTTDPAFRGRVMALYLAIFAGGTPIGAPIVGWVANVYGPRWALAIGAASGLIAAVIAG
                     FWMLRRRNRLIAEADVPRPVEDRELATQEIAIVESTTDRTS"
BASE COUNT         2362 a         5258 c         4763 g         2551 t
ORIGIN      
        1 agttcgcgta cgtcggccac cagatcggca acggcgaccc gtggctcgga gcgaactcgg
       61 ccgacgccct gaccttcctg catccgaccg ctgccggtcc ggccgtctac cgcgacgcga
      121 tcctggcagt gctggccagc tgaccgcggc gtagcacgcg tcaccgccag gcgccatggg
      181 ctcggcggat cccgcccgta tgcgtagggt ccggttatgg atgaaaccca cgcatacgac
      241 aacgaccgcg atgatgacga cggcggcaac gacgctcgcc accacgatgg tgcgacgacg
      301 gcgcacagcc ttcgatgggc gattgcgcgg acggagttct gacacgtcgt cgatcctagt
      361 cagcgcgccg gggcgtgggg tttgcactgg gactgcggag ggtgtaactt tgcactctcc
      421 gcaaaaatct tcctcgcact cctaccgtta aaggaaccat gtccagcgca ctctccaagg
      481 gcaagcagca ggcacaggca atcgccgacg ccggcggcgt gatgacccac cgccagatcc
      541 ttttcgtcat cttcggcctc atggccggaa tgttcctctc ctccctcgac cagaccatcg
      601 tgggcacgtc gatgcgcacc atcgccgatg acctcgacgg gctcagcctg caggcgtggg
      661 tgaccaccgc ctacctgatc acctcgacca tcgcgacgcc catctacggc aagctcagcg
      721 acatcttcgg tcgccgcccg ctgttcatca tcgcgatcgt catcttcctc atcgggtcac
      781 tcgccgccgg cttctcgacc tccatgtacg agctcgcggt cttccgcgcg cttcagggtc
      841 tcggcgctgg tggcctcatg gccctcccgc tcgcgatcat gggcgacatc ctcgcccccc
      901 gcgaacgcgc gaagtaccag ggctacttcc tggcggtgtt cggcgtctcc agcctgatcg
      961 gcccgctcgt cggcgggctc ttcgccggag ccgaccagat cctcttcatc accggatggc
     1021 gctgggtctt cctcgtcaac cttccggtcg gcatcatcgc gctggccatg gtgctgcgct
     1081 tcctgcacat tccgcacacc cgtcgcaagg tgcgcatcga ctggtggggg gccgcgaccg
     1141 tcgtcatcgc cctcgtgccg ctgctgctgg tcgccgagaa gggccgcgac tggggctggg
     1201 actcgttcta cgccatcacc tgctacatcg tcggtggatt gggtatcgcc gcgttcatca
     1261 tcagcgagaa gctcatgggt gacgacgccc tcatcccgct gaagctgttc aagcacccga
     1321 cgttctcgat gtccacggtg ctcggtgtac tcgtcggctt cggcatgttc ggcgccatga
     1381 tgacgatccc gctctacctg cagctcgtca acggcgcgac gccgaccgag agtggactgc
     1441 tgtcgcttcc gatgatcctc ggcctcatga tctcctcgat cgcgagcggc cagatcatcg
     1501 ctcgcaccgg acgctaccgg gtgttcccca cgctcggaac cgcgttcatg gcggtcggct
     1561 tcttctggct gacgttcctc acggccgaca agcccctcat ctggatcatg gtcgggatgc
     1621 tgctgatcgg cctcggtctg ggtcagctca tgcagaccct cacgatcgcc gcgcagaact
     1681 ccgtgggccc gcgggacatc ggcgtagcca ccagctcgtc gacgttcttc cgccagatcg
     1741 gtggcacgct gggcacggcg atcctgttct cggtgctctt cacgcgcatc ccgcagacca
     1801 tcgccgacgc gttcgcctcg acgagcgtga aggacggcat cgcggcggca gtggccgacc
     1861 caaaggtgct cgcggacccc gccaacaagc agatcctcga gctgttccag caggcgcaga
     1921 acggtggctc ggtcggcaac gcgctcgacg gcgacacctc gttcctcaac gggtctgacc
     1981 cgcgtctggc cgcaccgttc ctcgagggct tcaacaacgc cgccgtgagc gtctactggg
     2041 tcgcgctggc cgtcgttctc atcgcgttcg tgctgagctt cttcctcaag gcggctccgc
     2101 tacgagccaa gtccgctctc caggagaacg ccgatgcgga tgccgcggtc atcgcccagg
     2161 aggcggccga cctcgccggc gcgatggttg caccggacct ggacacgacg cacgaggcat
     2221 ccgagcccga gtctgcaccg accgggaagc gccccaagcg caccacctag cggacttttt
     2281 tccgcgcacg tcgagcggcg tccgaccccc gcatttccgc gggagtcgga cgccgcaagc
     2341 gtttacaggc tctggcgttc cgtgggagcg ctctcaacca gtacttgaca ggggttgcga
     2401 gcgctgccta gtgtgggccg cggttccgtg agagcgctct caaccaccac gtgagagcgc
     2461 tcccaacccg ccgggaccgc cctgcagtac ccaccacgca cacacaaagg agtgaccgtg
     2521 agaacatcac gacgcacaat cgccgcgacc atcgccggcg ccgcaaccct cgccctcgta
     2581 gtcaccggat gttcggcagg agggggcgac tcgagcgacg agccggtcac cctcaccctc
     2641 gcgacgttca acgacttcgg ctacaccgac gagctcctcg ccgagttcac cgcggagacc
     2701 ggcatcacgg tcatccacaa caaggcggcg acgtcgaacg acgctcgcgc caactacttc
     2761 cagaagctgg gcaactccgg tctggccgac gttgaggccg tcgagatcga ctggttcacc
     2821 gaggcgatgc agtactccga cctcctcgcg cccgttgccg acgacctcaa gggccgctgg
     2881 ctcgactgga aggaagccgc cgcgaccgac gctgccggca acctgatcgc gtacggcacg
     2941 gacatcggcc cgcagggcat ctgctaccgc tccgacctgt tcgccgccgc tggcctgccc
     3001 accgaccgtg ccgaggttgc gaagctgttc gatggtgact gggacaactt cttctccatc
     3061 ggcgcccagt acaccgccac gaccggcaag ccgttcatcg actccgccaa ctcggtgctc
     3121 cagggcatcg tgaaccagta cgagctcgca tacgaggaca ccgacggcag catcatcgcc
     3181 accaccaacc ccgacatcga ggccgcctac aaggctgtcg tcgagaaggc catcccgatc
     3241 tcggcctact cgggtcagtg gagcgaggac tggttcgcat cgatggccag cggcgagttc
     3301 gctgccatgc tctgccccgg atggatgctc ggcgtcatct cgggcaacgc tcccgacatc
     3361 acgggctggg acatcgctga cgcgttcccg ggcggcggcg gcaactgggg cggctcgtac
     3421 ctgaccgttc cggccaacgg cgctcacgtc aaggaggccg cggagctcgc tgcctggctg
     3481 acctcgcccg agacccagct caaggcgttc accaacgcgg gaaccttccc gagccaggtt
     3541 gacgctctcg ccagcgacga actcgccggc gccaccaacg agtacttcaa caacgccccc
     3601 accggcacga tcctcaccga ccgcgccaac gccatcaccg tggcgacctt caagggcccg
     3661 aagtacttcc agttccacga tgcactgcag aacgcagtga ctcgcgtctt cgacggcctc
     3721 gaagacgagc agacgagctg gaacaactgg gtcactgagg tttcggcctt ctagttcccg
     3781 aatgggggtg cgggtcgcca gcggcccgca ccctcgtcgt tccccgtccc gcgaactgat
     3841 ccgaaagcac ccccatgagt accgccaccg ccaagagccc gaagcaggtt cgggtcgtct
     3901 cgttctcgca ccggatgagc cgctgggacc tcaagctctc gccctacctg tacatctccc
     3961 ccttcttcct gatgttcctg gtcgtcggtc tcttcccgat catcttcacg gccgtgatct
     4021 ccttccagga ctgggatctc gtgcggggct ccggcaagtt cgtcggcttc gaccagtaca
     4081 tctggatcct tcagcagccc cacttctgga ccgcgctgcg caacaccttc agcatcttcc
     4141 tgctctcgac gatcccccag ctgctcctcg cgatcttcat cgcggcgatg ctggaccgga
     4201 acatccgggc caagaccttc tggcgcatgg gcgtgcttct gcccttcgtc gtcgcgcccg
     4261 tggcagtcgg cctcatcttc aacgtcgtct tcgccgacaa cttcggcctg gtcaacggat
     4321 tcctcaccga catcggtctg ccggccatcc agtggcacaa ggaaccgttc tggagccacg
     4381 tggcgatcgc caccatggtc aactaccggt ggaccggcta caacacgctc atcctgctcg
     4441 catcgatgca ggcggtgaac cgtgagtact acgaggctgc gacggtcgac ggcgctggcc
     4501 ggttccggca gttcctgtac atcacgctgc cgtcgctgcg gccgaccctc atcttcgtcg
     4561 tcatcacgtc gaccatcggc ggccttcaga tcttcgacga gccgcgcgtg ttcgaccagt
     4621 tcgggcgcgg cggcgccggc cagcaatggc tgaccatcac cctgtacctc tacgacatcg
     4681 ggtggggcca atggaacttc ggtcgagcag ccgccatggc gtggatcctc ttcgtcatca
     4741 tcctgctcat cggactgctg aacctcctca tcacgcgcac cctggtgcgc gatgagggca
     4801 cccgcgtggt caaactgagc cgccgtgaac gcaaggagct cgcacgatga gcacgacacc
     4861 ccccatcgct atcgtcgagg agagcatccc caacgagcgc cagcgtcgcc gcggcgagcg
     4921 caccacgcgc atccgcggca accgacccgg cttcgtcgtc tacgccgtgc tcgcggtcgt
     4981 cttcatcagc gcggcgttcc cgctctggtg gtcgttcgtg atcggctccg gggatgcctc
     5041 gtcgctgcgc gagcgcccgt ggtggccggg cggcaacttc ctcgcgaacg ctctcgccgt
     5101 cgtgtccaac cccgctgtca acttctgggc agcgctctgg aactcgatct actcgtcgtt
     5161 cctcatcgcg gcgtcggtgg tcatcacctc cacgctggcg ggctgggcat tcgccaagct
     5221 gcgtttcctc ggtggaccgg ccctgctggc gttcgtcgtc gcgaccatgg ctgttcctca
     5281 gcagctgggt gtcgtgccgc tgtacatcct gttctccgac ctcggctgga cgggatcgat
     5341 cggcgccatc atcatgccgg cgttgacgag cgcgttcgga gtgttctgga tgacgcagta
     5401 cctgcgccag gccgtgccca acgagctcat cgaggcagct cgagtcgatg gcgcaaccat
     5461 gatccgtacg ttctggactg tgggcgttcc cgctgcccgg ccggccgcgg ccatgctgtt
     5521 cctgttcatc ttcgtgggtg cctggaacaa cttcttctgg ccgttcatcg tgctcgaccg
     5581 gcagaacccc acactgcccg tcgcactgtc gctgctgcag tcgaactact tcgtcgacta
     5641 ctcgatcgtg ctcgccggcg tcgtgctcgc caccgttccg ctgctgctgc tcttcatctt
     5701 cgccgggcgc cagctggtga gcggtatcat ggctggcgca gtcaagggtt aacgggccgt
     5761 gactctacat ctcagagagg actctgtcgg catgtccgca gacgctcacc gtacgttccc
     5821 gcccaccttc ctcttcggcg cggcaaccgc ggcctaccag atcgaaggcg ctgcccacga
     5881 agacggccga accgactcga tctgggatgc cttctgccgc gtgcccggcg cggtggtcgg
     5941 tggcgacaac ggcgatgtgg cctgcgacca ctaccaccgc taccgcgatg acgtcgcgct
     6001 gatggccgag ctggggctgc agacctaccg cttctccacc tcgtggtcgc gcgtgcggcc
     6061 cgacggcggc gcggtcaacg cccagggcct ggacttctac tcccggctcg tcgatgagct
     6121 gctggcgcag ggcatcatgc cctggctgac cctctaccac tgggacctgc cgcaggcgct
     6181 cgaggagaag ggcggctgga ccaaccgcga cacctcctac ctgttcgcgg agtacgcggc
     6241 gagtgttcac gacgccctcg gcgaccgcgt caccgcctgg accacactga acgagccgtg
     6301 gtgcgcgtcg ttcttgagct acatcggcgg cgagcacgca ccaggtcggc aggaccctgc
     6361 ggcggggctc gcggcatccc accaccttct tctggcccac gggctggccg tgcaggagct
     6421 gcgctcccgc gactcgagcc tgcagctcgg catcaccctc aacctcaccg tcgccgaccc
     6481 ggtcgacccg tcagacgagg gtgacctcga cgccgcccgt cgcatcgacg gtcagttcaa
     6541 ccgctggttc ctcgacccga tcttccgcgg gtcgtacccc gaggacgtgc ttcaggatgt
     6601 cgcgggcctc ggtttcgagt cggtggtgca gccgggcgac ctcgagatca tctcctcccc
     6661 catcgacgcg ctcggggtga actactacca cggcgaggcc gtgagctcga agcctgccga
     6721 gcacgagatc accactcagg cgccgtcggc gcggccgaag cgctccccgt tcccggcggc
     6781 cgactcggtg cactggcacc gacgcgacct tccggtcacg gcgatggact gggaggtgca
     6841 gcccgagggc ctcacgcgcc tgctgatccg cgtgcacgag gagtacacct cgggtgccgg
     6901 agtctcgctc gcggtcaccg agaacggcgc cgcctacacc gacgtggtcg ctgccgacgg
     6961 cgccatccac gatgccgatc gcgtcacgtt ccttcagtgg cacctcgccg caattctcga
     7021 cgcgatcgac gccggagtgc cggtgagcgg gtacttctac tggtcgctga tggacaactt
     7081 cgagtgggcc tggggctacg acaagcgctt cggaatcgtg cgcgtcgact acgacactca
     7141 ggcgcgtact ccgaaggaca gcgcaatcgc ttacacccgc ataatcactg ggcgcgcgct
     7201 gcccgattct gctggctaga ctgcccccat gaccctgcat tcgccagccc gacgggtggc
     7261 gcccacgctc gaggccgtgg ctgcggaagc cggggtttcg aggtcgaccg tgtctcgtgt
     7321 ggtcaacggc tcggagcacg tgcgaccgga tgtcgtgacc gccgtcacag cggccatcga
     7381 acgactgaac tacgttccga accgggccgc ccgaagcctc gcgaaccgcc agaccatggc
     7441 catcgccctc gtcgtgcccg aggacaccac gaggttcttc ggcgacccgt tcttctccga
     7501 gatcgtgcag ggcatcaccc agggtctcga agacagcgac tacgtgctca acctgcaact
     7561 ggcgagcccg tctgccccat ccgagaagac catccgctac ctgctcggcg gcaatgtcga
     7621 tggagcgatc gtcgtgtcgc accactcggg cgacgacttc ttcacgacgc tcgatgccac
     7681 aatccccgtg gtgttcggtg gccgaccctt ccaccccgag gctcaccaga actcgttcgt
     7741 cgacgtcgac aaccgcgccg gcggtgccct gggcacccag tacctgatcg atctcggcag
     7801 gcgacgcatc gccacgatcg cgggtcccga gaacatgcag gcccccatcg accgccatca
     7861 gggctggctc gatgccgtga ccgccgcggg ccttcccacc gacctcgtcg tgcacggcga
     7921 cttcaccctg gccagcggcg ccgccggtat gcgcgagctg ctcgaccggt ccggcgagat
     7981 cgacgggctg ttcgtggcca gcgacctgat ggccaccgga gccatctccg tgctgcgcga
     8041 gcggggcatc cgcgttcccg aggatgtcgc cgtggtcggg ttcgacaaca gctcggccgc
     8101 gaccagcggg tcgatcggca tcaccacggt gcaccagccg tcgcgcgaga tgggcgccga
     8161 gatggcgcgg atgatgctgg ccatgctgcg cggcgagcgt cccgagcgcg agcgcgtgat
     8221 gccgacgcgc ctcgtcgtgc gcgacagcgc ctgatcggtc gcctcggtag gctgtcgtca
     8281 tggccagcga ggtgattcag gacgtgtcga tgaatcgctt cgagctgctc gtcgacggcg
     8341 aacaggtcgg actgtctgac tatcgcatcc gcgacgactc gatcgtgatc ctgcacaccg
     8401 agatcgaccc gtcacgccgt ggagagggcc tcggtgacct gctcgctcgc ggcatgctca
     8461 acctgattcg cgccgacacc gaccatcggg tggtgccgct gtgcccgttc atgaagaagt
     8521 ggatcgatga gcacaaggac taccaggatc tgctgacgcg ctagagcatt ggtggttgag
     8581 tagcgcgaag cgcgtatcga aacctaggcg tcgtccctgg gcttcaggtc gatcttgacg
     8641 ttgcccttgt agatgtcctt gtcgccgtcg ccgggcagcg gcgcgggcgc cggaagcatc
     8701 gtctggcggt cgagttcgag ctgggactcg taccgcgacg ggttgaagac ctcgtcaccg
     8761 atgcccatga caccgccgcc ggccttgccc gaggtgcggc tcttgttgct caggtcgatc
     8821 cagccgtagt gctgcgccac gaatccgagc gcgatgagga ccgccgctat gcctaagaag
     8881 atgagccagt ccacgaggca agcttacgtt ccgcgcgtcg gaaccacgac cggccgcccg
     8941 ccgaccaggt gcagctcgac cggcagtccg tagacgcgct cgatgcgctc ctcggtgagc
     9001 acctcggcgg gcgagccgat cgcctcgaga cccccggaag cgaccagggc gaggcggtcg
     9061 gcgtaggcac tcgcgagcga caggtcgtgc accaccacga caacggcgcg accctcggca
     9121 gcgagcccgc gggcgatgcg catgacgtcc tcctgatgcc tgaggtcgag ggatgccgtc
     9181 ggctcatcga gaaacacggt gcccgcgcgt tgcgccagaa cccgggccag cgagacacgg
     9241 gcgcgttcac cgccggagag ggcggtgtag cggcgggtgg cgaggtgacg gatgtctgtg
     9301 gcatccatcg cctcggtgac ggcccgcacg tcgtcgcggc cctcgagggt tcgcgcccag
     9361 ggggcgcggc ccatcgccac gacctcggag acgcggaacg ggaagctcac cgagttctcc
     9421 tgcgtgagca cgctgcgcag acgcgccagt tcggtgtgac ggatgccgcc gagctcacgc
     9481 ccgccgatcg acactgttcc gcgggtgggc cgccgttcgc cgctgagcac cgcgagcagg
     9541 gtggacttgc cggcgccgtt gggcccgacc aggcacagga cctcgccggg gatgacctcg
     9601 aggctgacac catccaggac ggttacgccg tccaggtcga ctcccacctc ggctgccgtg
     9661 atcacccgcg acgagtaggg gctcatgccc agcccccgct ctggcggcgc tggcggaaga
     9721 gcaggaagaa gaagaacggt ccaccgacca gcgaggtcag caggccgatc ggaaggtcgg
     9781 caccgggcac gaccgagcgc gcgagcaggt cggcgaagac gagcagtgct ccaccgccga
     9841 aggcgctcgc gacgatgagg gggcggtgcg aggggccgat cgccatgcgg atcacgtggg
     9901 gcacgacgag gcccacgaag gcgatgatgc ccacgaaggc cacggcgacg ccggtgagca
     9961 gggcgaccag cacgatcgat ccgacccgca ggtgctcgac atccaccccg aggtggcgtg
    10021 cattgcgctc gcccagcgcc agaaggtcgt agcgcttgcc gagcagcaca gcaatgactg
    10081 tgccgaccac gccgacgacg gccacgatga cgacttcgct ccagcgcgag ccgttgagcg
    10141 agccgagctg ccagaacacg atctgctcgc gactgccgga gtccgcaacg aagagcatga
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccccggcga atgcgttgat
    10321 ggcgattccc gtgagcagca gggtgacgac ctcggtgcgg ccgttggcgc gagagacgaa
    10381 gtagacgagc agcgtcgcgc caagaccgcc gaggaacgcg aagatcgcca agctgcccgt
    10441 tccgaaggcc gtgacgccga gcacgatcgc gatcgcggcg cctagcgcgc tgcccgacga
    10501 gacgccgacg acaccgggct cggcaagggg gttgccgaag atcgcctgca tcaccgcacc
    10561 cgccacggcg agcgccgccc ccaccgtgag cgccatggcg atccgcggga accgcacgac
    10621 ccacagcgtc gactcgacga tcgggtcggt cggtgcccac gagttcggga tgcccaccgc
    10681 ccgcaacacc gaccccacga cctcggcggg gctgaccgcc agctgcccga gcatcgcgga
    10741 ggcgacgaca cccaccacga gcagcactcc caggctcgtg gacagtacga cggcgcgcgt
    10801 cctcacggca cccgctgcag ccattcctcg gtaccgaact tctcggtaac gagctgctgc
    10861 gcttgggcca gctcctcggg cgtgatctcg ctcggggtga gtccgtagag accgcggaag
    10921 gtgccgacca tcttctcgat gatctcggct cgctgcagcc cggtctgcgt gcgcaggggg
    10981 tcgacgcgct tgttggcgct cttggtgccc ttgtcgctca tcttctcccg gccgatgcgc
    11041 agcacctcga ccatcttgtc gccgtccatg tcgtagctca tggtcacgtg gtgcagtacg
    11101 gcaccgctgc cgagacgctt ctgtgctgct ccgccgatct tgccggtggg gctggcgatg
    11161 tcgttgagcg gcacgtagct ggcctcgatg ccgagtgcct tgagagcgat gatcacccac
    11221 tcgtcgagga acgcgtacga gtcggcgaag ctcatgccct gcacgagctc ggtgggcgcg
    11281 tagatcgagt aggtgatgac cgatccggcc tccatgaaca tcgcgccccc gccggagatg
    11341 cgacgcacga tgtcgaagcc gtacttcttc gcgttgtcga gatcgacctc gttcttcacc
    11401 gactggaagc tgccgatcac gacggccggc ttctcccact cccagatgcg cagggttggc
    11461 ttgcggcgac cggcacccac ctcctcagcg agcacctggt cgagcgccat ctgcacgacg
    11521 gggtcgaggg cgggccccat gatgagctgc cagtcgtagt cccgccaccc cgtggcactc
    11581 gcgagcgcac ggcggatcgc cgtggctacg gcgtcggggg tgaagcccag gagggacgcg
    11641 tcatccggca gaccggcctg gatcgccttg gcgatgcgag cggcgtcggc atccgccggc
    11701 agaccgttca ctgccccgtt gatggccgcg agggccgagt cgggctcgag gaagaagtcg
    11761 ccggcaagac ggaagtcgtg gatgaccccg tcggtggttt cgaggtcgac gacgacgagc
    11821 ttgccgccag gaaccttgaa ctcgccgtgc atgaggcaag cctaattgtc cgccggttga
    11881 gtagcggccg aaggccgcgt atcgaaacca gccgtgattt cgatacgccg cttcgcggct
    11941 actcaatccg cgggcacgaa ctccccgtgc ccgttcgcga ccagccgggc acgcaggcgc
    12001 agggccgcgg cgtccaggtc ggcgggctcg gcgttgcgct ggatgcccgt gtggctgtac
    12061 tcgaagaccg tccacgatgg ccagacgtgc aggtgggagt gcggaaccat gtagccctcg
    12121 tacatgaccc cgacgcgggg cgagccccac tcctcgcgct gggcgagacc gatgagccgg
    12181 cccactcgga agatgtgctg ggcgacctcc tcgctgaggt cgagccagtt gtccacctcc
    12241 tcgagcggca tcaccagggt gtgccccggt ttgcgcggct cgatggtgag gatcgcgaag
    12301 caggtgtcat cccgccagat gaagcgaccc ggcgagttgc ctcgcaggat ctcggtgaag
    12361 acagaggtca tgcggtcagg cgcgcgtcca gccaggccac gaggtcggcg atgacctcgt
    12421 cgcggttcgt ctcgttgaag atctcgtggc gagcaccggg gtagacgatg gcctcgacgt
    12481 cggagagtcc cgagcgcgtg acatagtccg aggccagctt ggcgacactc ttctcgcccc
    12541 cgagcgagtc ggcgcttccg atcatgatga gaatcgggat gtcgcggccg agcttcttcg
    12601 ccggcttgcc gagcaggcgc agggcgtcac ccaggccgaa caacttgatg gtgtcggcgt
    12661 agaaggtctt cggatcgtcg gcgaaggcct tcgccaccga cgggtcacgg ctcagccact
    12721 cgttgggcgt cgagtacccc ttgttgaggt cgccgccgtt catgtcgccc agggtgcggt
    12781 aggcggtgcc agtcagaacg acggcctcgt agtcctcggc gtcggtgttg acgatcgtct
    12841 gcgccatgag ggagccccac gagtgtccga gcaacgcaag ggggagtccg gggttctcgg
    12901 cgcggatgat cgtggtgaac tgctgcagcg cggcgatggt cgcacgcaga ccgcccgggc
    12961 cgaggcgtcc gagtcgggac ttgtctccgg cgtgctgcgt gatgccggtc tcaccgtggc
    13021 cgcggtggtc gtccgcgtag acgctgtagc cggcctgagc gagggcctgt gcgacgtggt
    13081 cgtagcgcag ggcgtggtcg ccgagcccgt gggccagctg cacaacggcc ttcggcttgg
    13141 gcgccttcca cacgtagtag tgaatggtca cgccgtgggc gtcgacgtac gagtggtcgg
    13201 ttctctcaac ggtggtcttc ggcacggagc ctcctcgctc agccctatcg tacgggggcg
    13261 aaacagcctc cgcgctttag ttagcatggc taatttgcta ggctaactaa tcgtgactga
    13321 cgatctggac gacgagctgc gcattgcgat catgcggctg gcccgacgga tgcgcctgga
    13381 gcgcgccgac gaggacgtca ccgacggcca gctctcggtg ctcttcgttc tcaccaagca
    13441 cggggcgcag accctgggtg ccctcagcga gcacgagcgg gtcaccccgc cctcgatgaa
    13501 tcgcaccgtc aacgcgctcg agaacaatgg gctgatcgtg cgcgagtcct cccccgacga
    13561 cggtcgcaag gtgctcatca ccctcaccga tcgggcccgc gcactcgtcg ccgagactca
    13621 gcgccgccgc gtcgcctggt tctcccggca gctcgcctcg ctgtcagcag ccgagcgggg
    13681 ggcgctcgac gccgcagcgc cgatcctgcg caccctcgcc gactcgtgag tgccatgttc
    13741 cgcagtctgc gggtcttcaa ctaccgcctg tggttcgccg gcgctctcgt ctccaacacc
    13801 ggcgcctgga tgcagcgcac cgcccaggac tggatcgttc tcaccgaact gaccgaccgg
    13861 gatgccgcgg ccctcggcat caccatggcg ctgcagttcg gtccgctcct gctgctcatg
    13921 cccgtggcgg gactcatggc ggaccgcttc gaccgcaggc gcctgctcat gtggacccag
    13981 ggcatccagt gcgcgcttgc tctgggcctc ggcctgctgg tcgtctccgg ccacgcccag
    14041 ctctggcagg tgtacgtctt cgcgctgctg ctgggcatcg cgaccgctat cgacgctccg
    14101 gcacggcagg ccttcgtctc cgagctcgtc accgatgccg acctctccaa cgcggtcgcc
    14161 ctcaactcga cctcgttcca gagcgcccga ctggtcggcc ccgccgtcgc gggcgtgctc
    14221 atcgcgtgga tcggctccgg tcccgtcttc ctcatcaacg cgctgtcgtt cgcgggcgtg
    14281 ctcgcctcgc tcgccttcat ccgccgcaag gagctcgtcg cctcccctcg cctcatccgg
    14341 gctcgaggcc agatccgcga cggcctgcac tacgtgcgca cccgtggcga cctcgtcgtg
    14401 acgctggtcg cggtgttcct cgtgggaacc ttcggcttca acttccccat cttcatctcc
    14461 acgatgtcga cggtggagtt cggcaagggg gcgaccgagt tcggactgct gtcgtcgatc
    14521 atggcggccg gcgctgtgac gggatcgctg ctcgcggcgc ggcgaaccag ggctcgactc
    14581 gtggtggcgg tcgcggcatc cgctgcgttc ggactggcct gcgtgctggg tgccatcgca
    14641 cccacctact ggctcttcgc tgcgaccctg gtgctcatcg gcgttgcatc gctgacgatg
    14701 atgacgacgg cgaacgccta cgtgcagacc acgaccgacc cggcgttccg tggtcgggtg
    14761 atggcgctgt atctcgccat cttcgccggc ggcacgccca tcggtgcgcc gatcgtcggc
    14821 tgggtcgcca acgtctacgg cccgcgctgg gcgctcgcga tcggcgcggc atccggcctg
    14881 atcgcggcgg tcatcgccgg gttctggatg ctccgccgcc gcaaccggct catcgccgag
    14941 gccgacgttc cgcgccccgt ggaggaccga gaactggcca cgcaggagat cgcgatcgtc
    15001 gagtcgacca ccgaccgcac gagctgagcg ggct
//
LOCUS       DAOSOU010000023        14904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4043_length_14904_cov_7.012728, whole genome shotgun sequence.
ACCESSION   DAOSOU010000023 DAOSOU010000000
VERSION     DAOSOU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_14904_cov_7.012728"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..341)
                     /gene="ffs"
                     /locus_tag="PLW15_02270"
     ncRNA           complement(245..341)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLW15_02270"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            427..511
                     /locus_tag="PLW15_02275"
     tRNA            427..511
                     /locus_tag="PLW15_02275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:461..463,aa:Ser,seq:gga)
     gene            577..1197
                     /locus_tag="PLW15_02280"
     CDS             577..1197
                     /locus_tag="PLW15_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33257.1"
                     /translation="MASTTVRVAARGSVLRNTIYAAVAAGIPLFGVLYWLSIAQGNWL
                     RVLVVQAIYIGLFALIAVRHFGAYVEVTSTTVTKQALFVRSTLNRADVASSYLVDTHT
                     SNSAELLPQLLVLDAKGGRLMRMRGTFWSRDDMLRIAEAIGAPLTVETQPMTLKEFYA
                     LRPGTAYWYEGKLWLGIVGIVLAFGFAYLAVTWLMAAIGVPSVWSL"
     gene            1203..1799
                     /locus_tag="PLW15_02285"
     CDS             1203..1799
                     /locus_tag="PLW15_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33258.1"
                     /translation="MKRLRPHSHLFREGIIAVILFMAPVFVVLYVITLNNGHWLAVFV
                     LQVIVTLMVVLAAWGYFGVAIWVSPEAISERGFFGRKTTFSREQLGSIVIAETQTASS
                     AETTTQLFVCDEDGKGLVRMRGQFWSRENMELVRTTLDLPATEFDDAVTTRELRRDFP
                     QLLYGFERHPVLAALVFTAAIVVVAGLVMGFLFLAGVR"
     gene            complement(1806..3575)
                     /locus_tag="PLW15_02290"
     CDS             complement(1806..3575)
                     /locus_tag="PLW15_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOT33259.1"
                     /translation="MTDAAAQVEAAKKALAEAEAALAAATAAAAAATPAVVEPVETPS
                     VADKSSSTDGPLTADEVASIRAGYAFDGPALEMGALVNGGPLPDVPVRIPIAMVNRHG
                     LVAGATGTGKTKTLQVLAEQLSANGVPVFAADIKGDLSGIASPGEANDKLLERTRSIG
                     QDWKAIASPTEYYTLGGQGIGVPIRATVSSFGPLLLSKVLGLNPTQESSLGLVFHYAD
                     QAGLPLVDLSDLRAVLQWLVSDEGKPELKSLGGLSGATAGVILRELISFADQGADVFF
                     GEPEIDTALFLRTNDQGKGIVSLLEIPGVQDKPALFSTFLMWLLADLFNDLPEVGDID
                     KPKLVFFFDEAHLLFNGASKDFIDSITQTIRLIRSKGVGIFFVTQTPKDVPADVLAQI
                     GSRVQHQLRAHTPDDAKALKATVSTYPTSGYDLKQVLTELAIGEAIITVMNEKGAPSP
                     VAWTRLRAPQGSMAPTPADRMQATVDASPLYGTYGTVVDRDSAREILAVKMNAAAEAD
                     ARREADKVAAAQAREAAKVKADYDRAVREIERSSKPKTTTRTTTRTTRQDDNPIGDFL
                     GSRGGQTVVREVLRGIFSTLKRK"
     gene            complement(3732..4316)
                     /locus_tag="PLW15_02295"
     CDS             complement(3732..4316)
                     /locus_tag="PLW15_02295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33260.1"
                     /translation="MIEPVEIRLYRPADRADVYNVCVRTAAAGGDATGIYSSDDLMPD
                     VFAGPYLAFQPDLAFVVDVGDRVAGYVIAAADTRAFVERVRQEWVPTFASKYPEPSPT
                     DAPIVAMGVDPERMLVPEVDEYPAHLHIDLLPELQGQGCGRALIDTLRTELARRGIPG
                     LHLTMDPANLPARAFYDRLGFVELPSGALGISTA"
     gene            complement(4345..5610)
                     /locus_tag="PLW15_02300"
     CDS             complement(4345..5610)
                     /locus_tag="PLW15_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOT33261.1"
                     /translation="MRILLVGAGGVGDAIAKIAARRDFFETMIVSDYDLSRAERTIAW
                     IEAKHGTQGGRFVAAQIDASNPDSVASVAREHGATHVMNAVEPKFVPTIFAGALAAGA
                     DYLDMAMSLSEPHPTNPHEETGVKLGDDQFAQAPDWETAGRLALVGMGVEPGLSDVFA
                     RYAADHLFSDIDELGTRDGANLVVRDEAGNEIFAPSFSIWTTIEECLNPPVIWEKDRG
                     WYTTPPFSEPEVFEFPEGIGPVECVNVEHEEVLLMPRWVDAKRVTFKYGLGNEFIGIL
                     KTLHQLGLDKTEPLKVRSANGPVMVAPRDVVAAGLPDPATLGPRMTGKTCAGLWVTGT
                     GVDGKAREVYLYHVSDNEWTMAEYESQCVVWQTALNPVIALELLSTGAWTGTGVLGPE
                     AFDAKPFLDLMALPVAEGGYGQAWGLREG"
     gene            complement(5607..6272)
                     /locus_tag="PLW15_02305"
     CDS             complement(5607..6272)
                     /locus_tag="PLW15_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT33262.1"
                     /translation="MSASRTPTPPPRRPALLAGIAALILVIGIGVIVTVRASPFEFDT
                     EWMEEVIEHRGEIWLVPSLVMNFLGGGWFGTLLVPIAVTVLLMALRRPWSALYFLVAS
                     ATSAVLVQVLKVTFGRARPSDILVHVDPGSFPSGHVANAATVAVAIAFIVGRAWVWVA
                     GAVYVLVMALSRTYLGAHWVSDTVGGALLGAAVSVLLFAAFAPRLGDDSWRRASSPDS
                     VEP"
     gene            complement(6343..8676)
                     /locus_tag="PLW15_02310"
     CDS             complement(6343..8676)
                     /locus_tag="PLW15_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT33263.1"
                     /translation="MTTTTSTSSPAHVARVEQLIASMSIEEKVAQLYGVWVGASGEGG
                     DVAPHQHDLSGDVDLDALLPHGLGQLTRPFGTAPVDPALGALSLVRAQERIVRAGRHG
                     IPAMSHEECLAGFAAWGATAYPVPLSWGASFNPALIESMASRIGSDMRSVGIHQGLAP
                     VLDVVRDARWGRVEETIGEDPYLVGTIASAYVRGLESSGVVATLKHFVGYSASRAGRN
                     LAPVSMGVRERADVMLAPFEMVIREAGPRSVMNAYTDTDGVPTAADESLLTGLLRDEW
                     GFEGTVVADYFAVAFLKLLHSTSGTWSEAAVAALTAGIDVELPTVHTYGQPLLDAVAS
                     GLIAESTIDRSLRRVLLQKAELGLLDPSWSAIPPVLVGKDLGDIEGLRGSVALDPADN
                     RALAAQIAEESVVLLRNDGLLPLASVGRIAVIGPTSDDPYAVLGCYSFPAHIGVQYPG
                     TQIGIELPTLLESVTREFPSATIVHELGTSIDGGETNGIEAAVAAARSADVTVLALGD
                     RAGLFGRGTSGEGCDVESLDLPGAQRDLLDALLDAGVPLIVTLLAGRPYALGSAPDRA
                     GAIVQTFFPGEEGTAALAGVLSGRVNPSGRLPVSVPATPGAQPSTYLASPLARASTVS
                     NIDPSAAYPFGAGIGYSSFSWEPDGDAVTEISTRETATIGLTVTNTSDRAGCEVVQLY
                     VHDPVASVVRPVQRLVGYTKLALEPGQTARIRVEVCAELASFTGRSGDRIVEPGELVF
                     GFGHSSADIPITATVVVTGEPRVVGFDRVLHPEWHVG"
     gene            complement(8682..>9362)
                     /locus_tag="PLW15_02315"
     CDS             complement(8682..>9362)
                     /locus_tag="PLW15_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT33264.1"
                     /translation="DPWVFEKYLAVFQNPNFWTQILNSTIVAGLTTAGVVVVGVMAAF
                     VIARYEFWGRTLLYSLFTAGLLFPLTVAVLPLFTMLRTFGLLGNLSAVIAPQIAFAIP
                     TTIIILVPFLRAIPAELEDAAMIDGASRFGFFWRVLLPLSWPGLVTVGVLAFVASWNA
                     YLLPLLLLGNPATATLPVGVQYFSTAYSQDTAGVLAFTSVAMIPALLFFTLAQRRIVG
                     GLTGAVKG"
     assembly_gap    9363..9462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9463..10319)
                     /locus_tag="PLW15_02320"
     CDS             complement(<9463..10319)
                     /locus_tag="PLW15_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT33265.1"
                     /translation="MSIGTTTAGSAVSAGPAPARKRTRGWRGPLFIAAFVAPALIVYV
                     TLVLLPVALAAVYSFFNWNGLGALERFIGFDNYARALSDPVFLSAISHNLGIVALSLV
                     IQGPLAILIALLLNRPLRGRTLIRTLIFVPYVLSEVVAGLAFRLILPPNGPFDGILTA
                     LGWAGDKPNWLADPAIAFWTLFVVLTWKYLGFAIILMLAGLQGVPEELGEAAAIDGAS
                     WWQIQRYVTLPLLAPTIRIWAFLSIIGSLQLFDMVWILTGGGPLNSTTTMATYMVDYG
                     NQRSQVGYG"
     gene            complement(10322..11629)
                     /locus_tag="PLW15_02325"
     CDS             complement(10322..11629)
                     /locus_tag="PLW15_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT33266.1"
                     /translation="MKNKTRTMRIAAVTAATALTFAALAGCAPASSDSDKVTITWWHN
                     GTGEPLLSFWEGVASEFEASHPNVNIEVTAYQNEELQRTLIPNQLRTGSGLDLYQQWG
                     AGELAAQVNAGYVKDITADVTAEVDALGGVVAPWQYDGKTYGLPFTFGIEGFWYNKDM
                     FAQAGITEVPTTLDDLYAAIDKLKAAGLVPIAVGAGDGWPAAHYWYNFALKSCSPEVL
                     QEAQVSYTFDDPCFLEAGNLLQEFIASNPFQEGFLGTSAQQGAGSSAGMLATGNAAME
                     LMGHWNPGVMGGILQEETGDAAATPPEFLGWFNFPGIDGAKGDPTAALGGGDGFSCSA
                     WAPPECVELLKYISSVDVQTRFAEQGAGIPVTPGAEAGLADVNLKQVLAGLQASSYVQ
                     LWLDTAYGPTVGGAMNEGIVNLFGGAGSPEDIVKGMKDAAATL"
     gene            11782..12954
                     /locus_tag="PLW15_02330"
     CDS             11782..12954
                     /locus_tag="PLW15_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT33267.1"
                     /translation="MASPLDRIDGVRQRNLATVLELVHRGNSPSRADMTALTGLNRST
                     IGALVSELAALGLVVETDPSASNRVGRPSPRVEPAPGVGVIAINPEVDAITAAVVGLG
                     ARVVQRVRREVATPPTPEQAAAIIVDLVAELRSGPAAGMRLDAIGLAIPGLVRSSDGE
                     VRWAPHLGWREVPFTRIVQDAVGLPTFADNDATVGAHAEWLFGAGRGVDELIYLNGGA
                     SGIGGGAIVRGLPLGGAHGYAGEFGQNRPGLRDASDRATPNGTLEDEVSRSRLLAAVG
                     LESADEPTLRDSLAASADVAVAAEIDRQQRILAVALANAINVFNPALVVLGGFLATLL
                     EWGNPALELAIQSSTVPAAWEGVSIRPAALGEDRLLIGAAELAFAPLLRDPARAAD"
     gene            complement(12951..13976)
                     /locus_tag="PLW15_02335"
     CDS             complement(12951..13976)
                     /locus_tag="PLW15_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT33268.1"
                     /translation="MTDTRTSPVNRVTLADIAGQASVSLSTISKVLNGRSDVSAPTRA
                     RVEALLAEHGYLRRKTGESTTGLVELVFHELEAAWSMEIIRGVENIAAEHGLSVVLTQ
                     SGSRHAPGPEWIEGVMRRRPVGVVLVFSDLPAEYRRALKLRAIPFVIIDPAGDPSPDV
                     PSIGSANWSGGLMATRHLIELGHRKIAAITGPDDMMCSHARIDGFRSAMSAAGLEVRP
                     DWVRFGDFHTAGGQFHGTQLLQGDDRPTAIFAGSDLQALGVLEAVRGLGLRVPEDLSL
                     VGYDDIPLAKWVSPALTTIRQPLKRMGEEATRLVLRMSRTPLDSIPRMDLATNLVVRE
                     STAPPAA"
     gene            14105..>14904
                     /gene="xylA"
                     /locus_tag="PLW15_02340"
     CDS             14105..>14904
                     /gene="xylA"
                     /locus_tag="PLW15_02340"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOT33269.1"
                     /translation="MTTPTKSDKFSFGLWTIGYNGADPFGGPTRAPLDVVHAVEKLAE
                     LGAYGLTFHDDDLYAFGSTDAERQKQIDRLTKALADTGLIIPMITTNLFSQPVFKDGG
                     FTSNDRAVRRFALRKVLRNIDLAAELGAKTFVMWGGREGAEYDAAKDIRQALERYREA
                     VNLLGDYVTDKGYDLRFAIEPKPNEPRGDILLPTVGHALAFINSLERPELVGLNPEVG
                     HEQMAGLNFAAGIAQALYHDKLFHIDLNGQRGIKYDQDLVFGHGDLHNA"
BASE COUNT         2464 a         4919 c         5072 g         2349 t
ORIGIN      
        1 cctcggccga cgccaggtag atgctcttcg ccacgttccc accctaggcg cgcgaccgtc
       61 acccgcacga aggacttaag tcacgcgggt gcgcgagatg ccgcggcggg ggctggcaga
      121 aaacggggga gcaagccggc catctcggga agtctcttac acagccgaga atcgcgaggc
      181 atcgggcgca gcgatcatcg atgtgcatcc cagccgcggg tgacccgctg cttgcccgaa
      241 aagaaataga gacccctcgc gcacccgcca gagcccggtt acccttgcta cgtttccgtc
      301 ctgggggagt tggcctggat ggcgccacgc gaggagccat caacgattct atcccgcgac
      361 gggcgggagc gcttcgaccg gctatgattc catgaggcat tccgcgaagc tgcggggtgc
      421 tcgccaggag aattcgccta gtggcctatg gcgcacgctt ggaaagcgtg ttgggtgaaa
      481 gccctcgggg gttcgaatcc cccattctcc gctgacgcac cagatactga ttcacgtttc
      541 ttataccgtg ggggcacagt ggtcgacgca agccggatgg ccagcacgac cgtccgggtc
      601 gctgcgcgcg gctcggtgct tcgaaacacg atctacgcgg ccgtcgccgc gggtatcccc
      661 ctgttcggcg tgctgtactg gctgtcgatt gctcagggca actggctgcg cgtcctcgtc
      721 gttcaggcga tctacatcgg tctcttcgcg ctcatcgcgg tgcgccactt cggcgcctac
      781 gtcgaggtga cctcgaccac ggtcaccaag caggcgctgt tcgtgcgcag cacgctcaac
      841 cgagcggatg tcgcatccag ctaccttgtc gacactcaca cttcgaactc ggccgagttg
      901 ctgccgcagc ttctggtgct cgatgcgaag ggcggccggc tgatgcggat gcgcggcacc
      961 ttctggtccc gcgatgacat gctgcgcatc gccgaggcga tcggggctcc gctgaccgtg
     1021 gagacccagc cgatgacgtt gaaggagttc tacgccctgc gtcccggcac cgcctattgg
     1081 tacgagggca aactgtggct cggcatcgtg ggcatcgtgc tcgcgttcgg attcgcctat
     1141 ctcgctgtca cgtggttgat ggcggcgatc ggcgtgccga gcgtctggag cctgtgaccg
     1201 gcgtgaagcg cttgcggccg cacagccacc tctttcggga gggaatcatt gcggtgatcc
     1261 tgttcatggc gccggtgttc gtcgtgctct acgtgatcac tctcaacaac gggcactggc
     1321 tcgcggtgtt cgtgctccag gtcatcgtca ccctgatggt cgtgctggcc gcgtggggct
     1381 acttcggggt cgcgatctgg gtgagccccg aggcgatctc cgaacgcgga ttcttcggca
     1441 ggaagaccac gttctcgcgc gagcaactcg gttcgatcgt gatcgccgaa acccagaccg
     1501 catcgagcgc ggagaccaca acacaactct tcgtgtgcga cgaggacggc aaggggctgg
     1561 tgaggatgcg cggtcagttc tggagccgcg agaacatgga attggtgcgc acgacgctcg
     1621 acctaccggc aaccgagttc gatgacgccg tcacgacccg ggagctgcgc cgcgacttcc
     1681 cgcaactgct ctacggcttc gaacggcacc cggtgctcgc cgccctcgtc ttcaccgccg
     1741 cgatcgtcgt cgtggcgggg ctggtcatgg gattcctgtt cctcgcggga gtgcgctgac
     1801 ccacgctact tgcgcttgag ggtggagaag atcccccgca gcacttcccg cacgacggtc
     1861 tgacccccgc gggaaccgag gaagtcaccg atcgggttgt cgtcctgacg ggtcgtgcgt
     1921 gtggtcgtgc gggtggtggt cttgggcttg gatgagcgct cgatctcgcg cactgcccga
     1981 tcgtagtccg ccttgacctt cgccgcctcg cgcgcctggg cagcggccac cttgtcggcc
     2041 tcgcggcggg catccgcttc ggccgcagcg ttcatcttga ccgcgaggat ctcgcgcgcg
     2101 gagtctcgat cgacgaccgt gccgtaggtg ccgtagagcg gtgacgcgtc gaccgtcgcc
     2161 tgcatccgat cggcgggtgt cggcgccatc gacccctgcg gggctcgcag acgcgtccac
     2221 gccacgggag atggcgcacc cttctcgttc atcaccgtga tgatcgcctc gccgatcgcg
     2281 agttctgtga gcacctgctt caggtcgtag cccgaggtgg ggtaggtcga taccgtggcc
     2341 ttcagcgcct tcgcgtcgtc gggcgtgtgg gcgcgcaact ggtgctgcac gcgcgacccg
     2401 atctgggcga gcacgtcggc gggtacatcc ttgggcgtct gggtgacgaa gaagatgccg
     2461 acacccttcg aacggatcag ccgaatcgtc tgggtgatcg agtcgatgaa gtccttggat
     2521 gctccgttga agagcaggtg cgcctcgtcg aagaagaaca ccagcttcgg cttgtcgatg
     2581 tcgccgacct cgggcagatc gttgaacagg tcggccagca gccacatgag gaaggtcgag
     2641 aacagcgccg gcttgtcctg taccccgggg atctcgagca ggctcacgat gcccttgccc
     2701 tgatcgttgg tgcgcaggaa cagcgccgtg tcgatctcgg gttccccgaa gaagacgtcg
     2761 gcaccctggt cggcgaacga gatcagctct ctcaggatga cgcccgccgt cgcaccgctg
     2821 aggccgccga ggctcttcag ctcgggtttg ccctcgtcgc tgaccagcca ctggagcacg
     2881 gcgcgcaggt cgctgagatc cacgagcggc aagccggcct ggtcggcgta gtggaagacc
     2941 agtccgaggc tggactcctg cgtcgggttg agtcccaaca ccttcgacag cagcagcggg
     3001 ccgaagctcg acactgtcgc gcggatcggc acgccgatgc cctggccgcc gagcgtgtag
     3061 tactccgtag ggctcgcgat ggccttccag tcctgaccga tcgacctcgt gcgctcgaga
     3121 agcttgtcgt tggcctcccc cgggctcgcg atgcccgaca ggtcgccctt gatgtcggca
     3181 gcgaagaccg gcactccgtt ggcggagagc tgctcggcga gcacctgcag ggtcttcgtc
     3241 ttgccggttc cggtcgcgcc ggcgaccaga ccgtggcggt tgaccatggc gatcgggatg
     3301 cgcacgggca cgtcgggaag cggaccgccg ttgaccagcg cccccatctc cagcgcgggg
     3361 ccgtcgaagg cgtagccggc gcggatgctc gcgacctcgt cggcggtcag ggggccgtcg
     3421 gtcgagcttg acttgtctgc tacggagggg gtctcgacag gctcgaccac cgccggcgta
     3481 gctgcggccg ccgccgcggt tgcggccgcc agggccgcct cggcctcggc cagggccttc
     3541 ttggcggcct ctacttgtgc tgcggcatcc gtcatgcagc cagtgtattg atgccgtctc
     3601 gttgataact cctgctgcag ggtgtcttgg gcccgcgcga gcgcccaagt ctcgggctgc
     3661 gacgccctgt caccggcaat ctgcaggagt tatcgacacg atgcgcagat gggcaagtac
     3721 cgcggaggga ctcaggcggt cgagatgccg agggctcccg agggtagttc gacgaagccc
     3781 agccggtcgt agaacgcgcg ggcggggagg ttggccgggt ccatggtcag atgcaggccc
     3841 gggatgccac ggcgcgccaa ctcggtgcgc agcgtgtcga tcagcgcacg tccgcacccc
     3901 tgcccctgta gctcagggag caggtcgatg tgcaggtgcg cagggtattc gtcgacctca
     3961 ggcacgagca tccgctccgg atcgaccccc atcgcgacga tcggagcgtc ggtgggagag
     4021 ggttcggggt acttggatgc gaaagtcggc acccactcct ggcgcacccg ctccacgaat
     4081 gcgcgggtat cggctgccgc gatcacgtag ccggcgaccc ggtcgccgac gtcgaccacg
     4141 aacgcgagat cgggttggaa cgcgaggtag gggccggcga acacgtcggg catgaggtca
     4201 tcgctggagt agatgcccgt ggcatcccca ccggcagcgg ccgtgcgcac gcacacgttg
     4261 tagacgtcgg ctcggtcagc tgggcggtag agccggatct cgacaggctc gatcaccggt
     4321 gcgacccctc gatcaccggt gcactcaccc ctcgcgcagc ccccaggcct gaccgtagcc
     4381 gccctcggcg accgggaggg ccatgaggtc gaggaacggt ttcgcgtcga acgcctccgg
     4441 cccgagcacg ccggtgccgg tccatgcgcc ggtggacagc agctcgagcg cgatgaccgg
     4501 gttgagcgcg gtctgccaga cgacgcactg gctctcgtac tcggccatcg tccactcgtt
     4561 gtcgctcacg tggtacaggt acacctcacg tgcttttccg tcgacgcccg tgccggtgac
     4621 ccagaggccg gcgcaggtct tgccggtcat ccgcggtccg agagttgcgg ggtcgggcag
     4681 accggctgcg acgacgtcac gcggggcgac catcacgggt ccgttggccg agcgcacctt
     4741 gagcggctcc gtcttgtcca ggccgagctg gtgcagggtc ttgaggattc cgatgaactc
     4801 gttacccaac ccgtacttga aggtgacgcg cttggcgtcg acccagcggg gcatgagcag
     4861 cacttcctcg tgctcgacgt tgacgcactc gaccgggccg atgccctcgg gaaactcgaa
     4921 gacctccggc tcgctgaacg gcggcgtcgt ataccagccg cggtccttct cccagatcac
     4981 gggtgggttc aggcactcct cgatggtcgt ccagatcgag aagctcgggg cgaagatctc
     5041 gttgcccgct tcgtcgcgca ccacgaggtt cgcgccgtcg cgagtgccca gctcgtcgat
     5101 gtcgctgaac aggtgatcgg ccgcgtagcg cgcgaacacg tcgctgagtc cgggttcgac
     5161 acccatcccc acgagcgcga ggcgaccggc ggtctcccag tcgggcgcct gagcgaactg
     5221 gtcgtcgccg agcttgacgc cggtctcctc gtgagggttg gtcggatgcg gctccgacag
     5281 gctcatggcc atgtcgaggt agtcggcccc ggcggcgagc gcccccgcga agatggtggg
     5341 cacgaacttg ggctcgacgg cgttcatgac gtgggttgcg ccgtgctccc gggctacgga
     5401 tgccacgctg tcggggttcg acgcatcgat ctgcgcggcg acgaatctgc cgccctgagt
     5461 gccgtgcttc gcctcgatcc acgcgatggt gcgctcggca cgggacaggt cgtagtcact
     5521 gacgatcatt gtctcgaaga agtcgcgacg ggccgcgatc ttcgcgatgg cgtcgccgac
     5581 gccgccggcg ccaacaagga ggattctcat ggctcgacgc tatcgggact cgacgctcgc
     5641 cgccacgaat catccccaag acgcggcgcg aaagcggcga agagaagcac cgaaacggca
     5701 gcaccgagaa gcgcgccgcc caccgtgtcg ctgacccagt gggcgccgag gtaggtgcga
     5761 ctgagcgcca tgacgagcac gtacacggcc cccgcgaccc acacccaggc ccgcccgacg
     5821 atgaacgcga tggcgacagc gacggtcgcg gcgttggcta cgtggcccga ggggaacgat
     5881 cccggatcga cgtgcaccag gatgtcgctg ggccgagctc gcccgaaggt gactttgagc
     5941 acctgcacga gaaccgcgct cgtcgccgag gcgaccagga agtacagagc cgaccagggc
     6001 cgcctcaacg ccatgagcag caccgtcacg gcgatgggaa ccaggagcgt gccgaaccag
     6061 ccgcctccga ggaagttcat gacgagcgag ggaaccagcc agatttcgcc gcggtgctcg
     6121 atcacctcct ccatccattc ggtgtcgaac tcgaatggac tggcccgcac cgtcacaatc
     6181 acgccgatcc cgatgacgag gatcagggcg gcaatgcccg caagcaaggc gggtcgacgg
     6241 ggcggtggcg tcggggttct cgatgcgctc acgtgctcat cgtagagagt gaggcggcgc
     6301 gagcggcgac gcagccctgc cagcctcgcc cggatgcgcc gactagccga cgtgccattc
     6361 cggatgcagc acccgatcga acccgacgac gcgtggctca ccagtcacga ccacggtcgc
     6421 cgtgatgggg atgtctgcac tcgaatgccc gaacccgaag accagctccc ccggctcgac
     6481 gatgcgatcg ccggaccgac cggtgaagct ggccagctcg gcgcagacct cgacccggat
     6541 gcgcgccgtc tggccgggct ccagcgcgag cttggtgtat cccaccagac gctgcaccgg
     6601 gcgcaccacg gacgcgaccg gatcgtgcac atagagctgc accacctcgc atccggcccg
     6661 atcgctcgtg ttggtcacgg tcaacccgat ggtggccgtc tcgcgggtgg agatctccgt
     6721 gaccgcgtcg ccgtccggct cccagctgaa gctcgagtag ccgatgccag caccgaaggg
     6781 gtaggcggcc gaggggtcga tgttcgagac ggtgctcgcg cgggcgagcg gggaggcgag
     6841 gtaggtggat ggctgggccc cgggagttgc cgggacgctc accggaagcc tgccgctggg
     6901 gttgacccgc ccgctcagga ctcccgcgag cgccgcggtg ccctcctcgc ccgggaagaa
     6961 ggtctgcacg atcgcgccgg cccggtcggg agcagagccg agcgcgtacg ggcggccggc
     7021 gagaagggtc acgatcaggg gcactcccgc atcgaggagc gcgtccagga ggtcgcgctg
     7081 ggcacccggc agatcgaggg attcgacgtc gcatccctcg ccgctggtgc cgcgcccgaa
     7141 caggccggcg cggtcgccga gcgcgagtac cgtgacgtcc gctgagcggg cagcagccac
     7201 ggcagcctcg attccgttgg tctcaccccc gtcgatcgac gtgccgagtt cgtgcacgat
     7261 cgtcgcggag gggaactcgc gggtcaccga ctcgagcagt gtgggcagct cgatgccgat
     7321 ctgcgtgccc gggtactgca cgccgatgtg cgcgggaaag gagtagcagc cgagcaccgc
     7381 ataggggtcg tcgctggtcg gtccgatcac cgcgatgcga cccacggatg ccagcggcag
     7441 cagcccgtcg ttgcgcagaa gaaccactga ctcctccgcg atttgcgcgg ccagtgcgcg
     7501 gttgtcggcg gggtcgaggg cgacgctgcc ccggaggccc tcgatgtcgc cgaggtcctt
     7561 gcccaccagc accgggggga tcgccgacca cgacgggtcg agcaggccca gctccgcctt
     7621 ctgcagcagc acccgccgca ggctgcggtc gatcgtggac tcggcgatga gaccggatgc
     7681 cacggcatcg agcagcggct ggccgtaggt gtgcacggtc ggaagctcca cgtcgatgcc
     7741 cgccgtcagc gccgccacgg cggcctcgga ccacgtgccc gacgtggagt gcagcagctt
     7801 gaggaacgcg actgcgaagt agtcggcgac aaccgtgccc tcgaagcccc actcatcacg
     7861 cagcagtccg gtgagcaggc tctcgtcggc ggccgtggga acgccgtcgg tgtcggtgta
     7921 ggcgttcatc accgaacgcg gaccggcctc acggatcacc atctcgaacg gcgcgagcat
     7981 gacatcggcg cgttcgcgaa cccccatgga gacgggtgcg aggttgcgcc cggcgcggga
     8041 ggccgagtag ccgacgaagt gcttgagcgt cgccacgaca ccgctcgact ccaacccgcg
     8101 cacgtaggcc gacgcgatgg tgccgaccag gtaggggtcc tcgccgatcg tctcctcgac
     8161 ccgaccccag cgggcatccc gaacgacgtc gagcaccggg gcgagcccct ggtggatgcc
     8221 gaccgaccgc atatcggaac cgatgcggga ggccatcgac tcgatgagcg ccggattgaa
     8281 gctggctccc cacgagagtg gcaccgggta cgcggtggcg ccccaggcgg cgaacccggc
     8341 cagacactcc tcgtgcgaca ttgccggaat gccgtggcgt cccgcgcgca cgatccgctc
     8401 ctgggcgcga accagggaga gcgcgccgag ggcggggtca acgggtgcgg tgccgaacgg
     8461 gcgggtgagc tgtcccagcc cgtgcgggag cagggcatcc agatcgacgt cgccactgag
     8521 gtcgtgctga tgcggggcga catcaccgcc ctcaccgctc gcgccgaccc acacgccgta
     8581 gagctgggcg accttctcct cgatgctcat cgaggcgatg agctgctcga cccgcgcgac
     8641 gtgcgcgggc gacgaggtgc tggtggtcgt ggtcatccgg gtcatccctt cactgcgccc
     8701 gtgaggccgc ccacgatccg acgctgggcg agcgtgaaga acaggagcgc gggaatcatc
     8761 gcgaccgagg tgaacgcgag aactccggcg gtgtcctgcg agtaggccgt ggagaagtac
     8821 tgcacgccga cgggcagcgt cgccgtcgcc gggttgccga gcagcagcaa cggcagcagg
     8881 taggcgttcc agctggcgac gaacgccagg acgccgactg tgacgagtcc cggccaggag
     8941 agcggcagaa gcacgcgcca gaagaagccg aagcggctcg caccatcgat catcgccgca
     9001 tcctcgagct ccgcggggat cgcccggagg aaaggcacga gaatgatgat cgtggtgggg
     9061 atcgcgaagg cgatctgcgg ggcgatgacc gccgacaggt tgccgagcaa cccgaaggtg
     9121 cgcagcatcg tgaagagggg aagcaccgca accgtcaggg ggaagagcag cccggcggtg
     9181 aacagggagt acagcagcgt gcgtccccag aactcgtagc gtgcgatcac gaacgccgcc
     9241 atcaccccga ccaccaccac acccgccgtg gtcaggccgg caacgatcgt gctgttgagg
     9301 atctgggtcc agaagttggg attctggaac accgcgaggt acttctcgaa gacccacggg
     9361 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgccgta tccgacctgc
     9481 gatcgctggt tgccgtagtc gaccatgtag gtggccatcg tggtcgtcga gttgaggggt
     9541 ccgccgccgg tgaggatcca gaccatgtcg aacaactgga gcgacccgat gatcgacagg
     9601 aacgcccaga tgcggatcgt cggggcgaga aggggaagcg tgacgtagcg ctggatctgc
     9661 caccagctgg caccgtcgat cgccgcggcc tcgcccagtt cctcgggaac accctgaagc
     9721 ccggcgagca tcaggatgat cgcgaagccg aggtacttcc aggtgagtac gacgaacagg
     9781 gtccagaagg cgatcgcggg gtcggccagc cagttgggtt tgtccccagc ccagcccagg
     9841 gcggtgagga tgccgtcgaa gggtccgttg ggcggaagga tgagcctgaa ggcgaggccg
     9901 gcgacgacct cgctgagcac gtacggcacg aagatgaggg tgcggatgag cgtgcgtccg
     9961 cgaaggggcc ggttgagcag cagcgcgatg agaatcgcga gcggtccctg gatgacgagg
    10021 gagagggcga cgatgcccag gttgtggctg atcgcactga ggaacacggg atcgctcagg
    10081 gctctcgcgt agttgtcgaa gccgatgaac cgctcgagcg caccgagtcc gttccagttg
    10141 aagaagctgt acacggccgc gagcgccacg ggcagcagca cgagtgtcac atagacgatg
    10201 agcgcgggcg cgacgaaggc cgcgatgaac aggggtcccc gccaaccgcg cgtgcgcttg
    10261 cgggcggggg ctgggcccgc ggaaaccgcg gacccagccg tagtggtacc gatcgacatg
    10321 gttagagggt cgccgcagcg tccttcatgc ccttgacgat gtcctccggg gagcctgcgc
    10381 ctccgaagag gttcacgatc ccctcgttca tcgcgccacc gacggtcgga ccgtatgccg
    10441 tgtcgagcca gagctgcacg tagctcgagg cctgcagacc ggcgagaacc tgcttgaggt
    10501 tcacgtcggc gagcccggcc tcggcaccgg gggtgacggg gatgccggca ccctgctcag
    10561 cgaaccgggt ctgcacatcg acgctcgaga tgtacttgag caactcgacg cactccggcg
    10621 gagcccaggc ggagcaggag aaaccatctc caccgccgag ggcggcagtc gggtcaccct
    10681 tggcgccgtc gatacccggg aagttgaacc agccgaggaa ctcgggcggg gttgccgcgg
    10741 catccccggt ctcctcctgc aggattcctc ccatcacgcc cgggttccag tggcccatga
    10801 gctccatggc cgcgttgccg gtggcgagca tgcctgcaga gcttccggcg ccctgctgag
    10861 cggacgttcc gaggaagccc tcttggaagg ggttggacgc gatgaactcc tgaagcaggt
    10921 tgcccgcctc gaggaagcag gggtcgtcga aggtgtacga gacctgggct tcctgaagca
    10981 cttcggggga gcacgacttg agcgcgaagt tgtaccagta gtgcgccgca ggccatccgt
    11041 cgccggcacc cacagcgatg ggcacgagac ccgcggcctt cagcttgtcg atcgcggcgt
    11101 agagatcatc gagcgtggtg ggaacctcgg tgatgcccgc ctgggcgaac atgtccttgt
    11161 tgtaccagaa gccttcgatt ccgaaggtga atggcaggcc gtaggtcttg ccgtcgtact
    11221 gccacggggc gacgacgccg ccgagcgcgt cgacctctgc ggtcacgtca gcggtgatgt
    11281 ccttgacata gcccgcgttg acctgggccg ccagttcgcc agcgccccac tgctgataga
    11341 ggtcgaggcc gcttccggtg cgcagctggt tcgggatgag ggtgcgctgg agttcctcgt
    11401 tctggtacgc ggtcacctcg atgttgacgt tgggatgcga cgcctcgaac tctgaggcga
    11461 cgccctccca gaacgacagg agcggttcgc cggttccgtt gtgccaccac gtgatggtga
    11521 ccttgtcgga gtccgaactc gccggcgcac aaccggcgag cgcggcgaac gtcagcgccg
    11581 ttgccgctgt caccgccgca attctcatgg tgcgtgtctt gttcttcatt gaacacctct
    11641 cgaaagtttc gacatctacg tcgaaagtga ttgggatgcc tgatactacg tcgagatcac
    11701 ttttggggtc aagcacaaca aatcccgacc acgcgggcgg ggtgatcaag gccccgccga
    11761 tcgcgtgact aaggtgggcg aatggccagc ccgctagatc gaatcgacgg tgtgagacag
    11821 cgcaatctcg cgaccgtgct cgagctcgtg catcggggca actcgccctc gcgggccgac
    11881 atgacggcgc tgaccggact caaccgctcg acgatcggag ccctggtctc cgagctggcc
    11941 gcgctcggac tcgtcgtgga gaccgatccg tcggcgagca accgtgtcgg tcggccctca
    12001 ccccgagtcg aaccagcgcc cggcgtcggg gtgatcgcca tcaatcccga ggtcgacgcg
    12061 atcaccgcag ccgtcgtcgg cctgggagcg cgcgtcgtgc agcgggtgcg gcgcgaagtg
    12121 gccacgcctc cgacccccga acaggcagcg gcgatcatcg tcgacctcgt cgccgagctc
    12181 cgcagcggtc cggccgccgg catgagactg gatgccatcg gcctggccat tcccggactc
    12241 gtgcgttcct cggacggcga ggtgcgctgg gctccccacc tcggctggcg cgaagtaccg
    12301 ttcacccgca tcgtgcagga cgccgtgggc ctgcccacgt tcgccgacaa cgatgcgacg
    12361 gtgggcgcgc atgccgagtg gctcttcggt gccggtcggg gcgtcgatga gctgatctac
    12421 ctcaacggtg gcgccagtgg catcggcggc ggcgcgatcg tgcgcgggct gccgctcggc
    12481 ggagcccacg gctacgcggg cgagttcggc cagaaccgac ccggacttcg ggacgccagc
    12541 gaccgcgcaa ccccgaacgg caccctcgag gatgaggtca gcagatcccg actgcttgcg
    12601 gcggtcgggc tggaatcggc cgacgagccg acactgcggg actcgctcgc ggcatccgcg
    12661 gatgtcgctg tcgccgccga gatcgaccgc cagcaacgga tcctcgcggt ggcactggcc
    12721 aacgccatca acgtcttcaa ccccgcattg gtcgtgctcg gcggatttct cgcgacgctg
    12781 ctcgaatggg ggaatcccgc gctggagctg gcgattcagt cgtcgacggt gcccgcagcc
    12841 tgggagggcg tgagcattcg cccggccgcc ctcggcgagg atcgcctgct gatcggcgct
    12901 gccgagctgg cgttcgcgcc gctgctgcgc gacccagcgc gcgccgcaga ctaggcggca
    12961 ggcggggcgg tgctctcgcg caccacgagg ttggtggcga gatccatgcg cgggatgctg
    13021 tccagcggtg tgcgactcat gcgcagtacc agccgggtcg cctcctcccc catccgcttg
    13081 agcggctgcc ggatggtcgt gagcgccggg ctcacccact tcgcgagcgg gatgtcgtcg
    13141 tagccgacga gcgacaggtc ctcgggcacc cgcagcccga ggcctcggac cgcctcgagc
    13201 accccgagcg cctgcaggtc gctcccggcg aagatggcgg tgggacggtc atccccctga
    13261 agcagttgcg tgccgtggaa ctgcccgccg gccgtgtgga agtcgccgaa gcgcacccag
    13321 tcggggcgca cttcaaggcc agcagcgctc atggccgagc ggaatccgtc gatgcgcgcg
    13381 tgcgagcaca tcatgtcgtc ggggccggtg atggcggcga tcttgcgatg acccaactcg
    13441 atcaggtgcc gcgtggccat cagccccccc gaccagttcg ccgacccgat cgacggtacg
    13501 tcgggtgagg gatcgccggc cggatcgatg atgacgaagg ggatcgctcg cagcttgagt
    13561 gcgcggcggt attccgctgg caggtcggag aacacgagca cgacgccgac cgggcgccga
    13621 cgcatcaccc cttcgatcca ctccggaccc ggggcgtgtc gactgccgct ctgcgtgagc
    13681 acaacgctca ggccgtgctc cgcggcgatg ttctcgacgc cgcggatgat ctccatcgac
    13741 cacgccgcct cgagctcgtg gaagaccagt tcgaccaggc cggtcgtgga ctcgccggtc
    13801 ttgcgccgga gataaccgtg ctcggcgaga agtgcctcga cgcgggcccg ggtgggggcc
    13861 gagacgtcgc tgcggccgtt gagcactttg gaaatcgtcg acagcgagac gctcgcctgg
    13921 ccggcgatgt ctgccagggt cacccggttc acggggctcg tgcgcgtatc cgtcatgctg
    13981 cgctcctcga aatgaatcct actgcgccac gaaagtatcg gatgacgcgt tgccaactga
    14041 gaatgcatgg gatatgttgg tgcccgcaac attcgttcga tgacgagcct tcgggagacc
    14101 caccatgacc acccccacga aatccgacaa gttctccttc gggctctgga cgatcggcta
    14161 caacggcgcc gacccgttcg gtggccccac ccgcgcaccc ctcgacgtgg tgcacgcggt
    14221 cgagaagctc gccgagctgg gcgcatacgg cctgacgttc cacgacgacg acctctacgc
    14281 cttcggctcc accgatgccg agcgccagaa gcagatcgac cgcctcacca aggcgctcgc
    14341 cgacaccggc ctgatcatcc ccatgatcac caccaacctg ttcagccagc ccgtcttcaa
    14401 ggacggcggc ttcaccagca acgaccgcgc cgtgcgtcgc ttcgccctgc gcaaggtgct
    14461 gcgcaacatc gacctcgccg ccgagctcgg tgcgaagacg ttcgtgatgt ggggcggccg
    14521 cgagggcgcc gagtacgacg ccgccaagga catccgccag gcactcgagc gctaccgcga
    14581 ggccgtcaac ctgctcggcg actacgtcac cgacaagggc tacgacctgc gcttcgcgat
    14641 cgagccgaag ccgaacgagc cccgcggcga catcctgctg ccgaccgtcg gccacgcgct
    14701 cgccttcatc aactcgctcg agcgccccga gctcgtcggc ctcaaccccg aggtcggcca
    14761 tgagcagatg gcgggcctga acttcgccgc cggcatcgcg caggcgctgt accacgacaa
    14821 gctcttccac atcgacctca atggccagcg cggcatcaag tacgaccagg acctcgtctt
    14881 cggtcacggc gacctgcaca acgc
//
LOCUS       DAOSOU010000024        14755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4094_length_14755_cov_6.286871, whole genome shotgun sequence.
ACCESSION   DAOSOU010000024 DAOSOU010000000
VERSION     DAOSOU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 14755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14755
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4094_length_14755_cov_6.286871"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /locus_tag="PLW15_02345"
     CDS             <1..1762
                     /locus_tag="PLW15_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT33270.1"
                     /translation="VSREPGAPLLHGDKGPETRIAAPERNTVAEFHGETGDVAAAIES
                     ADAVVSGVWQTQRVSHVALETHATRGWLDAEGGLVLRTSSQVPFLVRDELCHIFGLRP
                     EGVRVFTARVGGGFGGKQELLTEDLVTLAVLATGRAVQYEFSRRDEFTVAPTRHPMRV
                     AVTLAAASDGTLTAMSVDQLMDTGAYGNHGIGVMFHSIHESVSVYRCANKRVDAQSVY
                     TNNLPSGAFRGYGLGQVIFAIESAMDELARTLGIDPFELRRRNVIVPGDRMVVTNPDE
                     ESDLGMESYGLDQCLDLAEQALARGNGAQAPAGWAVGTGMAVAMIATLPPRGHFATGT
                     VTVLDDLTIEARVGTSEFGNGTTTVHAQLVATALGVTVDRVRIMQSDTDAVAYDTGAF
                     GSAGVVVAGKALYAAALSLRAQVDASGLQPGLTATATHDGTPRSVAFNVHAFRVAVDT
                     ATGAVQILQSIQTADAGTVLNPEQLRGQLEGGTAQAIGTALYEEMTLDGVGQVLTNAL
                     RNYHIPQFADVPVTEVYFAQTSDPLGPLGAKSMSEAPYNPVAPALANAVRDAIGVRPL
                     ELPMSRDRLWRMLQPSPAVV"
     assembly_gap    2087..2186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2189..3091)
                     /locus_tag="PLW15_02350"
     CDS             complement(2189..3091)
                     /locus_tag="PLW15_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOT33271.1"
                     /translation="MFEIADRLLAAVDAGKRLVVATAISIEGSSPRTVGTSMAFDGEA
                     AIGSIAGGCVEGAVVETCELVLADGIPRTVEYGVSDETAFSVGLTCGGQLRIHVQPLD
                     DALIDALRAGTAVEVCREFTEEPPVKRRMIVFGAMEFSAALAAAATTIGYRVTVCDPR
                     SLFATPERFPGVELAVQWPTGWLEREISTGSITDDTAICVLSHDARFDAELIALALAS
                     PAGYVGAMGSRNTHDRRVASLAERGVNDLTRLHSPIGLDLGVSTPAETAISILAEVLA
                     ARSHSSASPLRSTSGAIHGARVRA"
     gene            3179..3709
                     /locus_tag="PLW15_02355"
     CDS             3179..3709
                     /locus_tag="PLW15_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOT33272.1"
                     /translation="MTEHYEPVVRTTGTPAEPSTAEVDAQWIARAIELATANVASGGG
                     PFGALIVRDGGLIAEGQNRVTANLDPTAHAEVVAIRAACQSVGDFSLAGSTLYTSCEP
                     CPLCLSAALWARIDRVVYAADRDDAARGGFDDREFYELFSRPRSEWHIAIDSVRPDDA
                     SAPFDAWLAHGDRTDY"
     gene            3818..4369
                     /locus_tag="PLW15_02360"
     CDS             3818..4369
                     /locus_tag="PLW15_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT33273.1"
                     /translation="MGTPASGTTGIVLAAGAGTRAGGPKALLRMPDGTPWLELACAFL
                     TDSGCDRVVAVLGAGERIARPLVPVTAEVLVVADWEAGMSASLRAGLAAASGGAVLIT
                     LVDLPELPASVGRRVMAGSGPLRQATYAGAPGHPVFIAREHWEPAAASLSGDRGARRY
                     LVENSVSEIECGDLWNGLDRDLG"
     gene            4433..>5760
                     /locus_tag="PLW15_02365"
     CDS             4433..>5760
                     /locus_tag="PLW15_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOT33274.1"
                     /translation="MSTTITPASGGAVDGKGLKGGALGLLSSVVVGVASTAPAYSLAA
                     SLGFIVASGGALLAGVKAPGIVLLAFIPMYFIAVAYQELNKAEPDCGTTFTWASRAFG
                     PVTGWMGGWGIIAADVIVMANLAQIAGSYSFTFVGQLGLPGVAELADSTLWSTVAGLA
                     WIAIMTYICYRGIEISARLQYVLLSIEVVVLLVFAGFALTRVYTGTAESYSLIPSLDW
                     FNPFTLDFGSVIAPAMLIAIFIYWGWDTAVSVNEESDDPAKTPGRAAILSTVLLLATY
                     GVVTVAAVAFAGVGTEGIGLGAEANSSDVFSAIGPALFGDSVIGHILLLLLAASILTS
                     ASASTQTTILPTARTALSMAVYKAVPAKFARIHPKFLTPTWSTIGMGIVSAGFYLIFT
                     LISPTLLGALIGSIGLMIAFYYGLTGFACVWFYRKTLTTSFRNFVMRGLFP"
     assembly_gap    5761..5860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5959..6348)
                     /locus_tag="PLW15_02370"
     CDS             complement(5959..6348)
                     /locus_tag="PLW15_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOT33275.1"
                     /translation="MDDHLRQLLIEELHRARHGYGLAVEFAGEGSEFSPSCGDAVTVR
                     VGPDGFSWEGNGCAVSMAAASALGATSVDDFRMLVDDYLASVAPGAPVVPGELEPFAG
                     IGRFPLRARCATLAWRAARTALAAADA"
     gene            complement(6365..6982)
                     /locus_tag="PLW15_02375"
     CDS             complement(6365..6982)
                     /locus_tag="PLW15_02375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33276.1"
                     /translation="MSALPAARTATPADAEAITETIALAFANDPVWAPALAIASGDTA
                     HVVPFWRHFVDGALRFDLSTVIDGPDGEAAAVALWIAPGEAEMADDAVTSLETLVHSA
                     LDPAGLAPMLELWDRFDTNHPSDPPHAYLNLLATHPAHRGLGIAQGLLAESLARFDAA
                     GLPTYLESTNPANDHRYRRAGYERIGEFATPHTGSPVARMWRDAR"
     gene            complement(7136..8779)
                     /locus_tag="PLW15_02380"
     CDS             complement(7136..8779)
                     /locus_tag="PLW15_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOT33277.1"
                     /translation="MNPAKYLASLLPTWRDYRSLKRTWRGDLLAGVTVGIVALPLALA
                     FGVSSGAGAEAGLVTAIVAGLVAAVFGGSNIQVSGPTGAMVVVLGPIVVTHGPGVVAM
                     VSVMAGVVVLIGGLLKLGRVVSFIPWPVIEGFTLGIAVIIFLQQVPAAIGAKAGASTN
                     ALVAAVESFATVNPATLWWALGSVVVVAAIMVAAPRIHRQLPGSLIAIALVTVVATLA
                     SLPLARIGVLPSSLPAPGLPALDPAVVVSLVGPALTVAALAAIESLLSARVGASLADT
                     GAYEPDRELVGQGLASLASGLFGGMPATGAIARTAVNVRSGGRTRVAAITHSLVLLAV
                     VSLAATVVSGIPLAALSGVLMVTAVRMVSIPTVRAVVRSTRSDAIVFFITAIVTVSFD
                     LIIAVGIGIAVAAFFALRALARSGGVHREELPGAAQPGDERIALFRLDGALFFGAADR
                     MLDRVNEISNVTVVIIRMSQLQVLDATGARVITEMIHALERRGITVLVKGIQPEHLEL
                     ATRVGVIASLRDERHLFTELEPAIEHARSHVARAGLGSL"
     gene            complement(8776..9144)
                     /locus_tag="PLW15_02385"
     CDS             complement(8776..9144)
                     /locus_tag="PLW15_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT33278.1"
                     /translation="MTDPVATDPSSFFSTGHPLYEVKANLFKGLSHPVRVRVLEVLSA
                     SPSVAVGDLLADTGLEASHLSQHLAVLRKHNLVTAERRASQVYYSLAYPEVADLLTVA
                     KGLLVHILATTQRQLDAATS"
     gene            9256..10005
                     /locus_tag="PLW15_02390"
     CDS             9256..10005
                     /locus_tag="PLW15_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOT33279.1"
                     /translation="MTGRERWEHATEYPLLALGLAFLVAYALPIIDPDFPAPWTAVFF
                     AVEIVAWVAFAIDLVVRIAISHDRWRFIRTHPLDIAAVVLPILRPLQALRALSILFLS
                     ARGLHRILRNRVLTYVVIAAMGIWLIAGLAVTQAERGVVGANIHDVWEGWWWAFVTMA
                     TVGYGDVYPITIEGRLVAVGLVVTGIALVGTVTAYIASWFAAQTRAAELAIQAEIDES
                     EDKIDALARQVTALQKLIAERLPEGEKKPSD"
     gene            complement(10039..>10311)
                     /locus_tag="PLW15_02395"
     CDS             complement(10039..>10311)
                     /locus_tag="PLW15_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33280.1"
                     /translation="DPEVLLLDEPLAALDVEIRADVRAELATHLADYAGLTIVVTHSL
                     ADVAALAHDVIVLERGKATQRGSLAELTAAPATPYVERLTRPAPAG"
     assembly_gap    10312..10411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10412..10910)
                     /locus_tag="PLW15_02400"
     CDS             complement(<10412..10910)
                     /locus_tag="PLW15_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOT33281.1"
                     /translation="MRRSFLTPALWIPTGLAIALLGLPLVALIARAPWAQLPELLSRS
                     EVLQALGLSLATASIATVVSLVLGIPLAIVLARSADWPAVPRRLLRALVTVPLVLPPV
                     VGGVALLLLLGRRGLIGQFLPFQIPFSTLAVVIAQVFVAMPFLVLAVEGAVRASDRRF
                     ETAAAT"
     gene            complement(10900..11670)
                     /gene="modA"
                     /locus_tag="PLW15_02405"
     CDS             complement(10900..11670)
                     /gene="modA"
                     /locus_tag="PLW15_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT33282.1"
                     /translation="MRRPLVLAALATLALAGCTAAAPEPTPPTEGTLSGTVTVFAAAS
                     LTESFQAIADAFTAEHPGVEVTFNFGGSSGLATQIVEGAPVDVFAAASPATMTTVTDA
                     GLATDAHDFASNTLEIAVPTDNPGGVTGLADFANPDLAIALCAAEVPCGAAAAKALDA
                     AGIVASVDTYEQDVKAVLTKVELGEVDAGLVYRTDVLAAGAKVKGIEFPEAAGAIARY
                     PIAALTASANPDAAAAFVAFVLSDAGQQILQEAGFGAP"
     gene            complement(11667..12065)
                     /locus_tag="PLW15_02410"
     CDS             complement(11667..12065)
                     /locus_tag="PLW15_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOT33283.1"
                     /translation="MPVTDFTVKQAAALLGVSDDTLRRWADAGRLTLSGSPATVAGSQ
                     LAALAQELAAESALDDAFPHSRASARNRVTGIVTKVTKDTVMAQVELQAGPFRIVSLM
                     SSEAATELGLEPGSLAVASIKATNVVVEVP"
     assembly_gap    12216..12315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12374..13438
                     /locus_tag="PLW15_02415"
     CDS             12374..13438
                     /locus_tag="PLW15_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOT33284.1"
                     /translation="MDARYSRQEALPGFGAEGQRRLAAARVLVIGAGGLGSSVIPALA
                     GAGVGTLAIVDDDRVELSNLHRQTVHAMADVGHSKAESAAQKVAALNPEVRVRAIDER
                     LSSANALGLFAGFDLVVDGSDNFPTRYLADDAARLSGIPVVWGAVSQYGGQAGLSWAA
                     KGPTYRDLFPVPPAPGTVPSCEVGGVLPTTVAVIGAIMATEAIKVLTGIGSPAIGRAT
                     TYDALSGGFRTLDYAGDPSAEPVTGLIDYAAFCGLGDSISPAQLAARTDDFTLLDVRE
                     PWEVDVVALPGALSVPLGELELRLAELDPAKPVVAFCHHGIRSASARLLLADHGFTAS
                     HLAGGIDAWARELDPAMARY"
     gene            13438..14628
                     /locus_tag="PLW15_02420"
     CDS             13438..14628
                     /locus_tag="PLW15_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOT33285.1"
                     /translation="MSGHDHSWSVEEHAAHVAELLAPALAPRPETIPVNDALGRVTSA
                     AVLSPVDLPLFRNSQMDGFAVRSADVAPAPTTLPIVGEVAARPSSPPDLAPGTAVRIM
                     TGAVVPNGADAVIPVEDTTSDGGTVTISRGRSAGEFVRERGSDLRAGDELLPAGLRLG
                     SRHLAVLAAAGITEVAVIARPRVSIITTGSELVPPGATPVLGQVFDSNGTALAAAVRA
                     AGAVVAARAHVTDDPAELIAALAVPADLVLTSGGISMGDYEVVRELLEPRGARVGHVA
                     MQPGGPQATAVWEGVPVICFPGNPVSTQLSFEVFVAPLLRTVAGLPQAVRERRQLSEA
                     VVSVAGRRQFLRGRAAEGGRVVIASGPGSHLVAGLAASDLLVIIPEDVTALNAGDDVE
                     TWAL"
BASE COUNT         2165 a         5015 c         5089 g         2086 t
ORIGIN      
        1 ggtgtcacgc gagccgggtg ccccgctgct gcacggcgac aagggccccg agacccggat
       61 cgccgcgccc gagcgcaaca ccgttgccga gttccacggc gagaccggcg atgtcgctgc
      121 ggccatcgag tcggccgacg ccgtcgtgag cggggtgtgg cagacccagc gggtgagcca
      181 cgtcgcgctc gagacgcacg cgacccgggg ctggctcgac gccgagggtg ggctcgtgct
      241 gcgcacgtcg agtcaggtgc cgttcctcgt gcgcgacgag ctgtgccaca tcttcggtct
      301 gcgccccgaa ggggtgcggg tcttcacggc acgagtcggc ggcggcttcg gcggcaagca
      361 ggagctgctc accgaggatc tcgtcaccct cgcggtgctc gcgacggggc gcgcggtgca
      421 gtacgagttc agccgccgcg acgagttcac ggttgctccg acacgccacc cgatgcgggt
      481 cgcggtgact ctggccgcgg catccgatgg cacgctgacg gcgatgtcgg tcgaccagct
      541 catggacacc ggcgcctacg gcaaccacgg catcggggtg atgttccact cgattcacga
      601 gtcggtgagc gtctaccgct gcgcgaacaa acgggtggat gcccagtcgg tctacacgaa
      661 caacctgccg tcgggcgcct tccgcggtta cggtctcggc caggtgatct tcgcgatcga
      721 gtcggcgatg gacgaactcg cccgcactct cggcatcgac ccgttcgagc tgcggcgccg
      781 caacgttatc gtgcccggcg accgcatggt ggtcaccaac cccgacgagg agtcggacct
      841 gggcatggag agctacggcc tcgaccagtg cctcgacctc gccgagcagg cgctcgcccg
      901 cggcaacggc gcgcaagcac ccgcgggctg ggccgtcggc acgggcatgg ccgtcgcgat
      961 gatcgcgact ctgcccccgc gcgggcactt cgccaccggc accgtgacgg tgctcgacga
     1021 cctcacgatc gaggcgcggg tcggcacgtc ggagttcggc aacggcacga caactgtgca
     1081 cgcccagctg gtcgccaccg ccctcggtgt gaccgtcgac cgggtgcgta tcatgcaatc
     1141 cgacacggat gccgtcgcct acgacaccgg cgcgttcggg tcggccgggg tcgtcgtcgc
     1201 cggcaaggcg ctgtacgccg ccgccctgtc actgcgagcc caggtcgatg cgagcggact
     1261 gcagcccggc ctcaccgcga ccgccaccca cgacggaacg ccccgctcgg tcgccttcaa
     1321 tgtgcacgcg ttccgggtcg cggtcgacac cgccaccggc gccgtgcaga tcctccagtc
     1381 gatccagacc gcggacgccg gcacggtgct gaaccccgag cagctgcgcg gccagctcga
     1441 gggtggcacg gcgcaggcca tcggtactgc cctgtacgag gagatgacgc tcgacggcgt
     1501 ggggcaggtg ctcaccaacg ccctgcgcaa ctaccacatc ccccagttcg ccgacgtgcc
     1561 agtcaccgag gtctacttcg cccagacgag cgaccctctc ggcccgctcg gcgcgaagtc
     1621 gatgagtgag gcgccgtaca acccggttgc ccccgctctg gccaacgcgg tccgcgacgc
     1681 gatcggcgta cggccgctcg aactgccgat gtcgcgcgac cgcctctggc ggatgctgca
     1741 gccgagccct gccgtcgtct gagctcacga ccgcgtccga cctgggagcg agggggtaag
     1801 cggccccgac gcaacggcgc ggcatccggt gtcctactcg acagactccc cgaactcagg
     1861 acgtttgttt ggatacctga cgaatgtcct gagttcgggg agcgcgcgga agatccagga
     1921 cactgccagc gggacggcac gggccggcac gggacggcac gggacggcac gggccggcgc
     1981 tggaccggcg ctggaccggc actggaccgg cactggccgg ccgctagcgg gcctcaggcc
     2041 cgaacgcggg cgccgtgaat cgcaccggac gtcgagcgca acggtgnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnncctc aggcccgaac gcgggcgccg tgaatcgcac
     2221 cggacgtcga gcgcaacggt gacgccgagg agtgcgatcg cgccgccaac acctcggcca
     2281 gtatcgagat ggcggtctcg gcgggggtgc ttacgccgag atcgagcccg ataggcgagt
     2341 gcaggcgggt gaggtcgttc accccgcgct cggccaacga ggcgacccgg cggtcgtggg
     2401 tgttcctcga gcccatcgcc ccgacgtacc cggcgggcga cgcgagagcg agggcgatga
     2461 gctcagcatc gaaacgggcg tcatgactca gcacacagat ggctgtgtcg tcggtgatcg
     2521 agcccgtcga gatctcccgc tccagccacc cagtcggcca ctgcacggcc agttcgacgc
     2581 ccggaaaccg ctcgggggtg gcgaagagcg accgggggtc gcacaccgtc acccggtagc
     2641 cgatcgtggt ggccgccgcg gcaagcgcgg ccgagaactc catcgcaccg aagacgatca
     2701 ttcgccgctt gaccgggggt tcttcggtga actcgcggca gacctccacc gctgtacccg
     2761 cgcgcagggc gtcgatcagt gcgtcatcca gaggctgcac atggatgcgc aactgaccgc
     2821 cgcaggtgag ccccacgctg aaggccgtct catcgctgac gccgtactcg acagtgcgcg
     2881 ggatgccatc agccagcacc agctcgcagg tctcgacaac cgcgccctcg acgcacccgc
     2941 ccgcgatgct gccgatcgcg gcctcgccgt cgaaggccat cgaggtgccc actgtacgcg
     3001 gagagcttcc ctcgatcgag atcgccgtgg ccacgacgag gcgtttgccc gcatcgacgg
     3061 ccgcgagcag gcgatccgcg atctcgaaca tgacaccagc gtcgcacacc tagcgttccg
     3121 tcggcgctat gcccgtgaca cagtctgatt caatggtaga aaccgggaaa gagacgtcat
     3181 gacggaacac tacgagccgg tcgtgcggac gaccggcacg cccgccgagc cctcgacggc
     3241 ggaggtcgat gcgcagtgga tcgcacgcgc catcgagttg gcgaccgcga acgtggcatc
     3301 cgggggtgga ccattcggcg ccctcatcgt gcgcgacggc ggcctgatcg cggagggcca
     3361 gaaccgcgtg accgcgaacc tcgaccccac ggcccatgcc gaggtggtcg cgatccgcgc
     3421 cgcctgccag tcggtcggcg acttctcgct cgcaggctcg acactgtaca cgtcgtgcga
     3481 gccgtgcccg ctgtgcctct cggcggccct ctgggctcgc atcgatcgcg tcgtctatgc
     3541 cgccgatcgg gatgacgcgg cccgcggcgg tttcgacgac cgcgagttct acgagctctt
     3601 cagccgtccg cgcagcgagt ggcacatcgc catcgactcg gttcgaccgg atgacgcgtc
     3661 agcgccgttc gacgcgtggc tcgcgcacgg cgaccgcacc gactactgac ccgccggtga
     3721 tcgagcggca cccggtgatc gagcggcacc cggtgatcga gcttgtcgag atctcgcatc
     3781 agcggtcgtc tcgaccatct tctgtataac agtacccatg ggcacccccg cgagcggcac
     3841 gaccggcatc gtgctcgccg ccggcgccgg cacccgcgcg ggcggcccta aggcccttct
     3901 ccggatgccc gacggcaccc cctggctgga gctggcctgc gcgttcctca ccgactcggg
     3961 gtgcgaccga gtggtcgcgg tcctgggcgc cggcgagcgg atcgcgcgcc cgctcgtgcc
     4021 cgtcacggcc gaggttctcg tggtcgcgga ctgggaggcc gggatgtccg cctcgctgcg
     4081 cgcggggctc gccgcggcat ccggtggcgc ggttctcatc acgctcgtgg acctgccgga
     4141 actgccggcg agcgtcgggc gccgcgtgat ggcagggtca gggccgctgc ggcaagcgac
     4201 gtacgcgggg gcgccggggc atccggtgtt catcgcccgg gagcactggg aacccgccgc
     4261 agcgagcctg agcggtgacc gcggagcccg gcgctatctg gtcgagaact cggtctccga
     4321 aatcgaatgc ggcgacctgt ggaatggact cgaccgcgac ctcggatagg cgtatatttc
     4381 gttcacccgt gagcgactcc cgtgcgcccc actgacccgt caaggcggat acatgtcaac
     4441 aaccatcaca ccggcatcag gcggcgcagt cgacgggaag gggctcaagg gcggcgctct
     4501 ggggcttctc tccagcgtcg tcgtgggcgt cgcatccact gcaccggcct acagcctcgc
     4561 cgccagcctc gggttcatcg tggccagcgg cggtgcgctt ctcgcgggcg tcaaggctcc
     4621 gggaatcgtg ttgctcgcgt tcattccgat gtacttcatc gccgtcgcgt atcaggagct
     4681 caacaaggcg gagcccgact gcggcaccac cttcacctgg gcgtcacgcg cgttcgggcc
     4741 tgtcaccggc tggatgggcg ggtggggcat catcgcggcc gacgtcattg tcatggccaa
     4801 cctcgcgcag atcgcggggt cgtactcgtt cacgttcgtg ggccagctcg gcctacccgg
     4861 cgtggccgag ctcgccgaca gcacgctgtg gtcgacggtg gcgggcctcg cctggatcgc
     4921 gatcatgacc tacatctgct accgcggcat cgagatctcc gcccgtctgc agtacgtgct
     4981 gctgagcatc gaggtcgtcg tgctgctggt gttcgccggc ttcgccctca cccgggtcta
     5041 caccggtacg gccgagagct actcactcat tccgagcctc gactggttca accccttcac
     5101 cctcgacttc ggcagcgtca tcgcccccgc gatgctcatc gcgatcttca tctactgggg
     5161 ctgggacacc gcggtcagcg tcaacgagga gtcggatgac ccggccaaga ccccgggacg
     5221 cgccgccatc ctctcgaccg tgctgctgct ggccacctac ggggtcgtca cggtcgccgc
     5281 agtcgcattc gcgggcgtcg gcacggaggg catcggcctc ggcgccgaag cgaactccag
     5341 tgacgtgttc tccgcaatcg gtccggcact gttcggcgac tcggtgatcg ggcacatcct
     5401 gctgctgctg ctcgcggcat ccatcctcac gtcggcgtcc gcgtcgaccc agacgacgat
     5461 ccttccgact gcgcgcaccg cgctctccat ggccgtctac aaggcggtgc ccgcgaagtt
     5521 cgcgcgcatc cacccgaagt tcctcacccc gacctggtcg acgatcggca tggggatcgt
     5581 gtcggcgggc ttctacctga tcttcacgct catcagcccc acgctgttgg gcgcgctgat
     5641 cgggtcgatc ggcctcatga tcgccttcta ctacggcctc accggcttcg cctgcgtgtg
     5701 gttctaccgc aagacgctca cgacgtcgtt ccgcaacttc gtgatgcgcg ggctgttccc
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacctgtcga acctcccgga
     5881 gggccaggag gccatcgacg ccgtcacggg caagaaggtc accaagccga agaagtgagc
     5941 aggtccgccg tcggcgcgtt acgcgtcggc ggcggccagc gcggtgcgcg cggcacgcca
     6001 cgcgagagtc gcacaccgtg ctcggagcgg gaagcgtccg atgccggcga acggctcgag
     6061 ttcaccgggc accaccggag ccccgggggc gacgctggcg aggtagtcgt cgacgagcat
     6121 cctgaagtcg tcgacgctcg tggcacccag cgcggatgcc gccgccatcg acacggcgca
     6181 gccgttgccc tcccagctga agccgtcggg cccgacccgc acggtcaccg cgtcaccgca
     6241 actcggactg aactcgctgc cctctccggc gaactcgacg gcgaggccgt aaccgtggcg
     6301 ggcccgatgc agctcctcga tgaggagttg gcggaggtgg tcgtccaccg ttgcatcacc
     6361 gtcctcagcg tgcgtcacgc cacatccgcg cgaccggcga gcccgtgtgc ggagttgcga
     6421 actcaccgat ccgctcgtag cctgcgcggc gatagcgatg gtcgttggcg gggttggtcg
     6481 actcgagata ggtgggaagg cccgccgcat cgaaccgcgc cagactctcg gcgagcagcc
     6541 cctgcgcgat tcccagcccc cggtgggcgg gatgggtggc gaggaggttg aggtaggcat
     6601 ggggcggatc gctcgggtgg ttggtgtcga agcggtccca cagttcgagc atgggcgcga
     6661 gccccgcggg gtccagcgcc gagtgcacga gcgtctcgag ggaagtgacg gcgtcatccg
     6721 ccatctcggc ctcgccgggg gcgatccaca gggcgaccgc cgcggcctcc ccgtcggggc
     6781 cgtcgatgac cgtcgagagg tcgaagcgca gcgcaccgtc gacgaagtgc cgccagaacg
     6841 gcaccacgtg agcggtgtca cccgaggcga tggcgagggc cggtgcccaa acggggtcgt
     6901 tcgcgaaggc gagggcgatc gtctcggtga tcgcctcagc atcggcggga gtcgcggtgc
     6961 gggcggcagg caaggcgctc atgccgtcgc cgccgcagag tcgatgaggg caacggccgc
     7021 ggtggcgggc gggcgggtcg cgcagcggat cggtccggtt ccgggctgga gggcatccat
     7081 ggggtgggcg ggcctccgtg ttcgtcggtg gtgctgagag actgtcgggt ggtgcttaga
     7141 gactacctag tccggcgcgg gcgacatggc tgcgggcgtg ctcgatggcg ggctcgagct
     7201 cggtgaacag gtgccgctcg tcgcgcagcg acgcgatgac cccgacccgg gttgcgagct
     7261 cgaggtgctc gggctggatg cccttgacga gaaccgtgat tccgcgccgt tcgagggcgt
     7321 ggatcatctc ggtgatcacg cgcgcacccg tcgcgtcgag cacctggagc tgcgacatcc
     7381 ggatgatgac gacggtgacg ttgctgatct cgttgacgcg gtcgagcatg cggtcggcgg
     7441 caccgaagaa cagggcgccg tcgagccgga acagcgcgat gcgttcgtcg ccgggctggg
     7501 cggctccggg cagctcttcg cggtgcacgc cgcccgagcg ggcgagggcg cgcagggcga
     7561 agaatgcggc gacggcgatg ccgatgccca ccgcgatgat gaggtcgaac gagacggtga
     7621 cgatcgccgt gatgaagaac acgatcgcgt cggagcgagt cgagcgcacg accgcccgca
     7681 cggtcgggat cgacaccatc cgcaccgccg tgaccatgag cacgcccgag agggccgcga
     7741 gcgggatgcc cgagacgaca gtcgcggcca gcgagaccac ggccagcagc acgagcgagt
     7801 gcgtgatcgc ggcgacgcgc gtgcgaccgc cggagcgcac gttgacggcg gtgcgggcga
     7861 tggcgccggt tgcgggcatg ccgccgaaca gtccggacgc gagcgacgcg agtccctggc
     7921 ctacgagctc gcggtcgggc tcgtaggcgc cggtgtcggc gagggatgcc ccgactcgcg
     7981 ccgagagcag cgactcgatc gcggcgagcg cggccaccgt cagcgcgggc ccgacgaggg
     8041 agacgaccac cgcggggtcg agagcgggca gcccgggcgc tggcaacgac gacggcagca
     8101 ccccgatgcg cgcgagcggc aggctcgcga gcgtggcgac gacggtgacc agggcgatcg
     8161 cgatgagcga gccgggcagt tgacgatgga tgcgcggggc cgcgaccatg atcgccgcga
     8221 cgaccacgac gcttcccagc gcccaccaca gggtggcagg gttcacggtg gcgaaggatt
     8281 ccacggccgc gacgagtgcg ttggtgctcg ccccagcttt cgcgccgatc gccgcgggga
     8341 cttgctggag gaagatgatc accgcaatgc cgagggtgaa gccctcgatg accggccacg
     8401 ggatgaacga caccacccgg ccgagcttga gcagcccgcc gatcagcacg accacaccgg
     8461 ccatgacgct gaccatggcg acgacgcccg ggccgtgcgt gacgacgatc ggcccgagca
     8521 cgacgaccat cgcgccggtc ggccccgaga cctggatgtt cgagccgccg aacaccgcag
     8581 cgaccagccc cgcgacgatc gccgtgacga gccccgcctc ggcaccggcg cccgagctca
     8641 ctccgaaggc gagcgcaagc ggaagcgcga cgatgcccac cgtgacaccg gcgagaagat
     8701 cgccgcgcca ggtgcgcttg agcgagcgat agtcgcgcca ggtggggagc agagatgcga
     8761 ggtacttcgc ggggttcatg aggtcgcggc atccagttgg cgctgcgtgg tcgcgaggat
     8821 gtgcacgagc agacccttgg cgacggtgag caggtcggcg acctcggggt aggcgaggct
     8881 gtagtagacc tgactggccc gccgctctgc ggtcacgagg ttgtgtttgc gcagcacagc
     8941 gagatgctgc gagaggtggg atgcctcgag cccggtgtca gcgagcaggt cgcccactgc
     9001 gacgctcggg ctggccgaca gcacctccag cacccgtact cgcaccggat gcgacagccc
     9061 tttgaagagg ttcgccttca cctcgtagag cgggtggccg gtgctgaaga acgacgacgg
     9121 gtcggtcgca acggggtcgg tcatcgtgct ccttgattca tgactttata tttccatcat
     9181 atcgtggatg ctgcggcgcg cgctcggagc agaatccggg catcccagtc gctacgctgt
     9241 cgtcatggtg caggcatgac cggacgcgag cgctgggaac acgcgaccga gtaccccctg
     9301 ctcgccctcg ggctcgcctt cctcgtcgcg tacgcgcttc cgatcatcga ccccgacttc
     9361 cctgcgccct ggacggccgt gttcttcgcc gtcgagatcg ttgcgtgggt cgccttcgcg
     9421 atcgatctcg tggtgcgcat agcgatctcg cacgaccggt ggcggttcat ccgcacgcat
     9481 ccactcgaca tcgccgccgt cgtgctgccg atcctgcggc cgcttcaggc gctgcgcgcg
     9541 ctgtcgatcc tgttcctgtc ggcgcgcggc ctccatcgaa tcctgcgcaa ccgggtgctc
     9601 acgtacgtcg tcatcgcggc gatgggcatc tggctgatcg ccggtctcgc ggtgacccag
     9661 gccgagcgcg gtgtcgtggg ggcgaacatc cacgatgtct gggagggctg gtggtgggcc
     9721 ttcgtcacga tggcgaccgt cggctacggc gacgtctacc cgatcacgat cgaggggcga
     9781 ctggtcgcag tggggctcgt ggtcacgggt atcgcgctgg tcggcacggt gaccgcctac
     9841 atcgcgtcgt ggttcgcggc gcagactcgc gccgcggagc tggcgatcca ggccgagatc
     9901 gacgagtccg aggacaagat cgacgcgctg gcgcggcagg tgaccgcgct gcagaagctc
     9961 attgcggagc ggctgccgga gggtgagaag aagccgagcg actgaggcgc ggtttcgata
    10021 cggcgcttcc gcgcctactc aaccggccgg ggcgggccgg gtcagccgct ccacgtacgg
    10081 ggtggcgggc gccgcggtga gttcagcgag tgacccgcgc tgggtggcct tgccccgctc
    10141 aagcacgatc acgtcgtggg cgagcgccgc gacatccgcg aggctgtggg tgacgacgat
    10201 cgtgaggccc gcgtagtcgg cgaggtgggt ggcgagttcg gcgcgcacgt cggcgcggat
    10261 ctccacgtcg agggccgcga gcggctcgtc gagcagcagc acctccgggt cnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggtcgcgg ccgccgtctc gaagcgccga
    10441 tcggatgccc gcaccgcacc ctcgaccgcg agcacgagga acggcatcgc cacgaacacc
    10501 tgtgcgatca ccacggccaa cgtggagaag gggatctgga acggcaggaa ctgcccgatc
    10561 agtccgcggc gcccgagcag cagcagcagg gcgactccac cgaccaccgg cggcagcacg
    10621 agcggcacgg tcacgagcgc gcgcagcagt cggcggggca cggctggcca gtcggccgag
    10681 cgggccagca cgatcgccag cgggatgccc agcacgaggc tcacgacggt ggcgatgctc
    10741 gccgtcgcca gcgacaggcc gagcgcctgc agcacctcgc tgcggctgag cagctcgggc
    10801 agctgggccc acggcgcgcg cgcgatgagc gcgacgagcg gtaggcccag cagcgcgatg
    10861 gccagcccgg tgggaatcca cagggcgggg gtgagaaaac tacggcgcac cgaacccggc
    10921 ctcctgcagg atctgctggc cggcatccga gagcacgaag gcgacgaatg cggcggccgc
    10981 atcggggttg gccgacgcgg tcagcgccgc gatcggatac ctcgcgatcg cccccgcggc
    11041 ctccgggaac tcgatgccct tcaccttcgc acccgcggcc agaacgtctg tgcggtagac
    11101 gagcccggca tccacctcgc ccaactcaac cttggtgagc acggccttga cgtcctgctc
    11161 ataggtgtcg acggatgcca cgatgcccgc cgcatcgagc gccttggccg cagcagctcc
    11221 gcacggcacc tccgcggcac acagtgcgat cgccaggtcg gggttcgcga agtcggcgag
    11281 gccggtcaca ccgccggggt tgtcggtcgg caccgcgatc tcgagagtgt tggaggcgaa
    11341 gtcgtgcgcg tcggtggcga ggccggcatc cgtcaccgtc gtcatcgtgg cggggctggc
    11401 ggcggcgaag acgtcgaccg gggcaccctc gacgatctgg gtcgccaacc ccgagctgcc
    11461 gccgaagttg aaggtcacct cgacccccgg gtgctcggcc gtgaacgcgt cggctatcgc
    11521 ctggaacgat tcggtgaggg atgccgcggc gaacaccgtc acggtgccgc tgagcgtgcc
    11581 ctcggtcggg ggcgtcggct cgggtgcggc ggcggtgcag ccagcgagtg cgagggtcgc
    11641 cagggcggcc agaacaagcg gccgcctcac ggcacttcca ccacgacgtt cgtggccttg
    11701 atgctcgcga ccgcgagact ccccggctcg agaccgagct ccgtcgctgc ctccgaactc
    11761 atgagcgaca cgatgcggaa cggccccgcc tgaagctcca cctgcgccat gaccgtgtcc
    11821 tttgtcacct tcgtgacgat gccggtgact cgattgcgcg cgctcgcgcg gctgtgcggg
    11881 aacgcgtcat cgagcgcgga ctcggcagcg agttcctgcg cgagggcggc gagctgggag
    11941 cccgcgaccg tcgccgggct ccccgacagg gtcagccgcc ccgcgtctgc ccagcgccgg
    12001 agtgtgtcgt cgctcacgcc gagcagcgcg gcggcctgtt tcacggtgaa gtcggtcacg
    12061 ggcatcctca tctgcggtta gttgccgcaa ttctagctgc acttgcaact tcggtcgttc
    12121 ggcaaactgc gccccgtgtc cataactcct gcaatttcgg gtcggggtga ccactcctca
    12181 gcggaactcc gggctccgcc gcagcagccg ggccannnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnngacgg cggcagcgga cggcgcccgc ggagagggcg cacggcggtg
    12361 cgaagatgga accatggatg cccgctactc gcgccaggag gccctgccgg gcttcggcgc
    12421 cgaaggccag cggcggctcg ccgccgcccg cgtgctggtc atcggggcgg gcgggctcgg
    12481 gtcgagcgtc atcccggccc tcgcgggcgc cggcgtgggc actctggcca tcgtggacga
    12541 cgaccgcgtc gagctcagca acctgcaccg ccagaccgtg catgcgatgg cggatgtcgg
    12601 ccacagcaag gccgaatccg cggctcagaa ggtggcggcc ctcaatcccg aggtgcgggt
    12661 gcgcgcgatc gacgaacgcc tcagcagcgc gaacgcgctc gggctgttcg ccgggttcga
    12721 cctcgtggtc gacggcagcg acaacttccc gacccgctac ctcgcggatg acgcggcacg
    12781 gctcagcggc atcccggtcg tctggggtgc cgtctcgcag tacggcgggc aggccgggct
    12841 cagctgggcg gcgaaggggc cgacctatcg cgacctgttc cccgtgccgc ccgcccccgg
    12901 caccgtgccc agctgcgagg tcggcggggt gctgcccacc accgtcgcgg tgatcggcgc
    12961 gatcatggcg accgaggcga tcaaggtgct caccggaatc ggctcccccg cgatcggccg
    13021 ggcgacgacc tatgacgcgt tgagcggcgg attccgcact ctcgactacg cgggcgaccc
    13081 gagcgcggaa cccgtgaccg gactcatcga ctacgccgcg ttctgcggcc tgggcgactc
    13141 gatcagcccc gcccagctcg ccgcccgcac cgacgacttc accctcctcg acgtgcgcga
    13201 gccgtgggag gtcgacgtcg tcgcgctgcc cggcgcgctg tccgtgccac tcggcgagct
    13261 cgagttgcga ctcgccgagc tcgacccagc caagccggtc gtggcgttct gtcatcacgg
    13321 catccgctcg gcgagcgccc gcctgctact cgccgaccac gggttcactg ccagccacct
    13381 cgccggcgga atcgacgcct gggcccgcga gctcgacccg gcgatggcga ggtactgatg
    13441 tcgggccacg accacagctg gagcgtcgag gagcacgccg cccacgtcgc cgaactgctt
    13501 gcacccgccc tggcaccccg gcccgagacg atccccgtca acgatgccct gggccgcgtg
    13561 accagcgccg cagtgctctc gccggtcgac ctgccgctgt tccgcaactc ccagatggac
    13621 ggcttcgccg tgcggtcagc ggatgtcgcc cccgcgccaa caacactgcc gatcgtcggc
    13681 gaggtggcgg cccggccctc gtcgccgccc gacctcgccc ccggcaccgc ggttcgcatc
    13741 atgacgggcg ccgtcgtgcc gaacggcgcc gacgcggtca tcccggtgga ggacaccacc
    13801 agcgacggcg gcacggtcac gatctcccgc ggtcgctcgg ctggcgaatt cgtgcgcgag
    13861 cgcggcagcg atctgcgcgc gggtgacgag ttgcttcccg ccggcctgcg gctcgggtcc
    13921 cgccatctgg cggtgctcgc cgccgccggc atcaccgagg tcgccgtgat cgctcgtccc
    13981 cgggtgtcga tcatcaccac cggcagcgag ctcgtgcctc cgggcgcgac tccggtgctc
    14041 gggcaggtgt tcgactcaaa cggcaccgca ctcgcggcgg cggtcagggc ggcaggcgcc
    14101 gtcgtcgcgg cgcgcgcgca cgtcaccgac gaccccgccg agctgatcgc cgccctcgcc
    14161 gttcccgccg acctggtgct cacgtcgggc ggcatctcca tgggcgacta cgaggtggtg
    14221 cgcgaactgc tcgaaccgcg cggcgcccgg gtcggccacg tcgccatgca gccgggcgga
    14281 ccgcaggcga ccgcggtctg ggagggcgtg cccgtgatct gcttccccgg caacccggtg
    14341 agcacccagt tgtcgttcga ggtgttcgtg gcgccgctgc tgcgcaccgt cgcgggactg
    14401 ccgcaggcag tgcgcgagcg gcgacagttg agcgaggcgg tggtcagtgt cgcggggcgg
    14461 cggcagttcc tgcgcgggcg agcggccgag ggcggtcggg tggtgatcgc ctcgggaccg
    14521 gggtcgcacc tggtggcggg gctggcggca tccgatctgc ttgtcatcat tccggaagac
    14581 gtgacggccc tgaacgcggg cgatgacgtg gaaacatggg cactatgagt tcccagagtc
    14641 gcccggccca gagtcgcccg ggccagagcc agcccgcgct gagtcacgtc ggcccggacg
    14701 ggcgcgcacg catggtcgac gtcggcgaca aggccgtcac gcgtcgcagc gccac
//
LOCUS       DAOSOU010000025        14730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4104_length_14730_cov_6.784668, whole genome shotgun sequence.
ACCESSION   DAOSOU010000025 DAOSOU010000000
VERSION     DAOSOU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 14730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14730
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4104_length_14730_cov_6.784668"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLW15_02425"
     CDS             complement(<1..1033)
                     /locus_tag="PLW15_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HOT33286.1"
                     /translation="MTETHPLETVNAWWRAANYLTVGQIYLRDNALLTRPLVDADIKP
                     RLLGHWGTSPALNLVYAHLNHLITQKPLEVLYVAGPGHGGPAMVANAWLDETYSEIYP
                     RISQDAAGMRALFRQFSAPGGIPSHASPDLPGSIHEGGELGYSLVHAYGAALDDPNLI
                     VACVVGDGEAETGTLAASWHLNKFLNPATDGAVLPILNLNGYKIANPAVLARISEEEL
                     FSLFRGYGYEPVLVSGGFDGEDPMRVHEAFAEALTWATDRIAAIQTDARSGTETERPA
                     WPMIILRTPKGWTGPKVVDGLPVEDTWRAHQVPIATVRGNAEHLAQLEEWMRSYRPEE
                     LFDGDGRPVA"
     gene            complement(1033..1779)
                     /locus_tag="PLW15_02430"
     CDS             complement(1033..1779)
                     /locus_tag="PLW15_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT33287.1"
                     /translation="MSASIVVGIDGSGPSRAAFAWALARATSIGAAILAVHCIEESSD
                     SRGPEVLEETVSAARAATGDVQIRSELVHGAAPWELAAVARPGDLLVVGTHKTGYLNG
                     RVLGTRSVIVASVAPCSVVVVPDATLAGRAGILVGVAADGASDAAVLAGAREAARLGE
                     DLTLLHCTDATQTGRTVLSDAVALVATVAPDLEVRSRLSRRRPAEGLLDASRSASLLV
                     IGSSRRGMDHLGSVAYEVLLNINTTVMVAR"
     gene            1881..3575
                     /locus_tag="PLW15_02435"
     CDS             1881..3575
                     /locus_tag="PLW15_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT33288.1"
                     /translation="MTDELSFPDVSRSELDRTLSQLVENAQDVLNSQDRLRSLLKATR
                     VVAGELELAEVLKRIIEAAVELVGARFGAIGVIAPDGTLEQFIHVGLPDELVGPIGRL
                     PEGHGLLGALIDEQEPIRLDHLAEDPRSSGFPPGHPAMDSFLGVPVRVRDVVYGNLYL
                     SESTAGSFSAEDQELLVALAATAGGAIDHARLFDETRRRQRWASALAEVTAALLSDDV
                     QDSLGIVAERITTLAEADLVTFLGAASPTTLRVELARGPLAPGVEGLVFDAQGTVAGR
                     AQESGQPVLSDAAAASALGDGVRLGPTMAIPLITSDGPNGVLTVSREPGRSRFTEVDL
                     GTAADFARQASVALRLAAGRADRLRLATLEDRSRIARDLHDHVIQRLFGAGLSLQALA
                     GSLPDEASRAKLVDQVDALDAAIAEIRTAIFTMTTPQDAERPSLRHRVIDILGESDGL
                     FEQPPVLSFSGAVDTMIDVALADDVAAVVREGLSNVARHAAAHNVTIAVGFAAGSVTV
                     RIDDDGTGIDPSAAVRASGTANLAQRAEARGGSFTLEPREPRGTTLTWIARVESTE"
     gene            3572..4192
                     /locus_tag="PLW15_02440"
     CDS             3572..4192
                     /locus_tag="PLW15_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33289.1"
                     /translation="MKRVFLVDDHEIVRRGVADLINAEADLTVVGEASTVKQALSRIA
                     ATTPDVAVLDVRLPDGSGIDLCRDIRSAHPDVACLMLTAYDDDEASYTAVLAGAAGYV
                     LKDIRGQGLLESIRRVANGEILVDPAVTKRVVARATTGADAAADLSLRERQVLDQIAA
                     GLTNRQIAEHLGLAEKTVKNYVSGLLAKLGMERRTQAAVYRANQTP"
     gene            4202..4564
                     /locus_tag="PLW15_02445"
     CDS             4202..4564
                     /locus_tag="PLW15_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33290.1"
                     /translation="MTSITSIPQKPGRVWGVLALIAVLFPLPWLFVLNVLSVVVRSQP
                     PRTGTSAEEWAYGLIAFGGLFFFPLFAILTVFFAIRAVLKPRVAGKVMGWIAIALLVL
                     SIPLIWNGYAVWIMAGAR"
     gene            4561..4965
                     /locus_tag="PLW15_02450"
     CDS             4561..4965
                     /locus_tag="PLW15_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33291.1"
                     /translation="MSDFDPRFDPAFQRGYDGPAVTPPATTDAPAPAAPPLVRIDPQA
                     AVAPQAEQDELPPRRGNPFLVALAVIGVALAVGGLVLVFQLRTIFGDGNGNPDFDYVT
                     LQTLMIAAPILTGLGVATGIGVLFVFAVRWGR"
     assembly_gap    4968..5067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5150..5647
                     /locus_tag="PLW15_02455"
     CDS             5150..5647
                     /locus_tag="PLW15_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT33292.1"
                     /translation="MSERVQLDLDAFVASEYPKVVAAVGLITGNRQDAADAVQDAIVG
                     FLAKPPTHPVTNLAAWFTVVASNRVKDGYRSEAARQRAVAKLGMPVEETTDAIELLDV
                     DVANALKSLPLQQRQVCALHYLMDQSIETIAEGLGVSEGTVKTQLHRARKALAARLRK
                     EDHHG"
     gene            5640..6338
                     /locus_tag="PLW15_02460"
     CDS             5640..6338
                     /locus_tag="PLW15_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33293.1"
                     /translation="MADIDVLLSASLKRIAQPGDAAGVADAIRARVDAGDTGTPATAS
                     GFGARPWWLFLLSGLLVILVAVTGVSVALGQPSPVTPTPDAASVAVSSSPTSSPTLTP
                     TPTATPTATAAPEPAPAPAEPDPEPAPPADTTAPVVTVPTSSQPQLFSANGGSTIITA
                     LATDDTGVVAVAISWTGVASGSASMVPGWSYTFVLPPASGPPPSGSITFTVQAFDAAG
                     NASDPVSVVVPVLP"
     gene            complement(6335..6904)
                     /locus_tag="PLW15_02465"
     CDS             complement(6335..6904)
                     /locus_tag="PLW15_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOT33294.1"
                     /translation="MPVAGGLTVVARAVVGGALGVVAALVVGVVVASAQSTPSAASVT
                     PIPADAQPMIVREVLSGDTVVLSNPRPGPQVPDWGAVSVRLIGVYAPHFGLINECYAV
                     ESQGRLRQLLPKGSLAWVATDATAKDDNGRWLMYVWTSNGEFVNLALADEGFVTAVSS
                     YDNESHFSAIAQATEQAFRRHAGLWGACS"
     gene            complement(6883..7455)
                     /locus_tag="PLW15_02470"
     CDS             complement(6883..7455)
                     /locus_tag="PLW15_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOT33295.1"
                     /translation="MVVRAVVGGVVGAAAIVAALSSAWTQPTAHGASGASRPADAQPM
                     IVRTVLSGDTVVLTSARPGAQVQLWGDLTVRLIGLDAPDLAPVVECFAAEAQGGLGAM
                     LPEGSLAWVTTNEQGDSGGIWSMYVWDSEGRFVNETLAEQGLARASDFSERGPLYPLI
                     AQATEEAYRRGQGLWGWHESAQSPCQWPEA"
     gene            complement(7532..8089)
                     /locus_tag="PLW15_02475"
     CDS             complement(7532..8089)
                     /locus_tag="PLW15_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33296.1"
                     /translation="MVVRVTLGVVLALGLLTWAIVSLWARGAESVESPVAQPPDAQPM
                     LVDEVLSGDTVVLTSTRAGAQVPLFGRLTARLVGLDAPDLGRRFGTDVDQCFAPEALA
                     RLEDLLPAGSIVWVTTDAPTRDPSGDWLLNVWGSDGRLVNYLMAVGGYAEVPDSAADT
                     RYGQILADGEHQALVSFGGMRGACR"
     gene            complement(8109..9494)
                     /locus_tag="PLW15_02480"
     CDS             complement(8109..9494)
                     /locus_tag="PLW15_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT33297.1"
                     /translation="MKWRLVAALIATTLLVILVQDIPLSYYIQGVQRDRIITGLERDA
                     FVLAGRSEEALESPTPESIAVITEVAKRYQASSGARVIVVDGKGIAVVTSDPDDSKIG
                     ASYLSRPEIGTALTGEIASGTRYSQTLQQELLYVTVPVFSGSRILGAVRLTYPAQVVD
                     DAVATQLRQLWVVALTTVLLAGVVGYVVAAGVTRRLKMLRASTERLADGHLDERADEG
                     AGASELRSLSRSFNVMAERLDGLIAQQRSFAADASHQLRTPLTALRLKLERAHDLIHT
                     DPDGAAERLAAAESEADRLGTIIEGLLLLSRTEAKSAVAVPVDVAAAARARVDHWLPL
                     SDESGVAIRYEGATQAVASVVPTATEQILDNFIDNALSVSPRGTTIVVRVESRGDRVA
                     LHVLDQGPGLSLEDCARAFDRFWRASSQVGGSGLGLAIVAQLAEASRGSAKLEPRPGG
                     GLDASVLFPAD"
     gene            complement(9511..10188)
                     /locus_tag="PLW15_02485"
     CDS             complement(9511..10188)
                     /locus_tag="PLW15_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33298.1"
                     /translation="MRVLVVEDDASVADGILDGLAQANVDALHVSTGQGGLDALSSYQ
                     PDLVLLDLGLPDMDGTEVCRRIRAVSQTPIIVVSARDDEIDRVVALEMGADDYVVKPF
                     GMRELVARIRAVARRTGPTVAEPAAARTFGALVVDTRSQKVTLAGEPVHLTAKEFELL
                     VYLSADPGAVFRRSEILHDVWDTNWYGTTKTLDAHIAAIRKKLGDPGWIESVRGVGFR
                     FGEPGAQ"
     assembly_gap    10475..10574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10628..10978
                     /locus_tag="PLW15_02490"
     CDS             10628..10978
                     /locus_tag="PLW15_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33299.1"
                     /translation="MTKSHPAGGTRTLVGLISTFALVGGVVGFQNAASAEEAAVQQQA
                     LLGRGVGVASARVAPTEAPVAEAAPADSAPASDPVPAADPAPAAAPAPVVDPAPAPAP
                     APAPPSGTTGGSGG"
     gene            10979..11944
                     /locus_tag="PLW15_02495"
     CDS             10979..11944
                     /locus_tag="PLW15_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT33300.1"
                     /translation="MELDAIARGALLAAHAQNDSQPPTYSDRRPLMGGHATITLVGGG
                     EDLVARCFALADHLEARWSRFLPTSDISRLNWAEGASVEVDPVTVQLISAMREASELS
                     DGDFDPTLLPELIASGYAASVVAPDLVTTLPASASAPGRLASLTLDGSSVRMPVGTTL
                     DPGGIGKGLAADIICEFGLAEGAWGIMAEISGDIVVAGRAPDAVAWLLGVEDPFDTDR
                     HLTTVRIARGAIVTSSQRKRRFDTAAGVRHHLIDPRTGASALTDIQTVTVIAATGVRA
                     EAISKSGFLRPVEEYLAWLPQVGAAALLVDSAGRQHVSANWGRYA"
     gene            11941..13476
                     /locus_tag="PLW15_02500"
     CDS             11941..13476
                     /locus_tag="PLW15_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT33301.1"
                     /translation="MSLNDPHIWWYVTRASALIAWALMTLSVVWGILLSTRILRRVDN
                     PGWLQDLHRFLGGLSVIMVLLHMVTLMLDGWLHLSLVQVLVPFTSDFKPIAVALGIVA
                     FYLLIAVQGSSMIMHRLPRTFWKGLHYASYAVVLLVSFHAGLSGTDVGSLGYKILAFS
                     LIGLTAAATVVRIAVTNRTTRAAKAEVWVPPHDPAPRISPPRIQTRTMVVADSGLIAE
                     GVMGIRLVALGGGLLPIWYPGAHVTLHLANGLERQYSLCGDPGERHHYDLAVLRTEDS
                     AGGSRWVHESVVPGTTIEVSGPLNHFELEPSREYLFIAGGIGITPIKAMIESLPAIRE
                     WRLIYVGRSRRSMAFANELAERYPDRVRIHASDESPAQLDFTRLVTPDTTEVYCCGPE
                     SLMSAVASAVPSARLHAERFIPIERVPSIPDQPLRLTFSRSPLALTVGAGETVLDALE
                     RGGMPVSGSCRKGVCGTCEVKVLAGTPEHLDSVMDDAEKDKLKIMYPCVSRALTPELV
                     LDF"
     gene            complement(13532..14305)
                     /locus_tag="PLW15_02505"
     CDS             complement(13532..14305)
                     /locus_tag="PLW15_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT33302.1"
                     /translation="MPEGDTVYRSAKNLNAALAGQVLTRCDIRVPAFATVDLTGETIE
                     GVASRGKHLLMHVGEYTLHSHLKMEGSWHLYRHGSKWFRPEFQARAILGTAEFLAVGF
                     DLGEFEVVRRDDEDSVVGYLGPDLLGDWQPEQALANLRANPEREIGLALLDQRNLAGL
                     GNVYRAELCFLRGVLPRRPVGEVPELEKMVELAWRLIDANKDRAQRTTTGQLNGQRLW
                     VYGRTGKPCLRCGTPIQHGELGDTALTQRDVWWCPNCQV"
     gene            complement(14467..>14730)
                     /locus_tag="PLW15_02510"
     CDS             complement(14467..>14730)
                     /locus_tag="PLW15_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33303.1"
                     /translation="AGALVVLVDGALTLYVERGGKTVLTFGSDADALAAASRSLTSTV
                     RTALGKLKVERIDGEFAVGTPLGLALVEAGFVATPQGLRLRNA"
BASE COUNT         2200 a         5112 c         4961 g         2257 t
ORIGIN      
        1 tcgccaccgg gcggccatcg ccgtcgaaca gctcctcggg gcggtagctg cgcatccact
       61 cctcgagctg ggcgaggtgc tcggcattgc cgcgtacggt cgcgatcgga acctgatgcg
      121 cgcgccaggt gtcctcgacg ggtaggccgt ccacgacctt cggtccggtc caccccttcg
      181 gagtgcgcag gatgatcatc ggccaggccg ggcgttcggt ctcggtgccg ctccgggcat
      241 ccgtctggat ggcggcgatg cggtcggtcg cccaggtgag ggcctccgcg aacgcctcgt
      301 gcacgcgcat cgggtcttcg ccgtcgaagc cgcctgacac cagcacgggc tcgtagccgt
      361 aaccgcggaa cagcgagaag agctcctcct cggagatgcg cgcgagcacg gccgggttgg
      421 cgattttgta gccgttgagg ttcagaatcg gcagcacggc accgtcggtc gcggggttga
      481 ggaacttgtt gaggtgccag ctcgcggcga gcgtgccggt ctcggcttcg ccatcgccga
      541 cgacacacgc gacgatgagg ttcgggtcat ccagggcagc accgtaggcg tgcacgagcg
      601 aatagccgag ctctccgccc tcgtggatcg acccgggcag gtcgggggag gcgtgcgagg
      661 ggatgccgcc cggcgccgag aactggcgga acagggcgcg catgccggcg gcgtcctgcg
      721 agatgcgcgg gtagatctcg gagtaggtct cgtcgagcca cgcgttggca accatcgccg
      781 gccccccgtg ccccgggccc gcgacgtaga gcacttcgag gggcttctgg gtgatcagat
      841 ggttgaggtg cgcgtagacg aggttcaacg cgggcgaggt gccccagtgc cccagcagtc
      901 gcggcttgat gtccgcatcg acgaggggtc gggtgagtag cgcgttgtcg cgcaggtaga
      961 tctggccgac cgtgaggtag ttcgcggcac gccaccaggc gttgacggtc tccagcggat
     1021 gtgtttcggt cattatcggg ccaccataac tgtcgtgttg atgttgagga gaacttcgta
     1081 ggcaactgag ccgagatggt ccatgccgcg ccggctcgac ccgatgacaa gcagcgaagc
     1141 cgagcggctc gcatcgagca ggccctcggc cggcctgcga cgggagaggc ggctgcgcac
     1201 ctcgagatcg ggggcgaccg tcgcgaccag ggcgacggca tccgagagca cggtccggcc
     1261 ggtctgggtc gcgtccgtgc agtgcagcag tgtcaggtcc tcgcccagac gcgcggcctc
     1321 gcgagccccc gcgagaacgg cggcgtcgga ggcaccgtcg gctgccacgc cgacgaggat
     1381 tcccgcgcgg cccgcgagcg tggcatccgg caccacgaca acactgcagg gggcgacgga
     1441 tgcgacgatc acactgcgcg tgccgagcac ccggccattg aggtaccccg tcttgtgcgt
     1501 gcccacgacc aacaggtcac cggggcgcgc cacggccgcc agctcccacg gggcggcacc
     1561 gtgcacgagt tcggagcgaa tctgaacgtc gccggtcgct gctctggcgg cgctcacggt
     1621 ctcctcgaga acctcggggc cgcgggagtc ggaactctcc tcgatgcagt gcacggcgag
     1681 aatcgcggca ccgatcgacg tggcacgggc gagagcccac gcgaaggcgg cacggctcgg
     1741 ccctgagccg tcgatgccga cgacgatgct cgcgctcatg gcgtgtcctt cctcgggcac
     1801 tgcgcccatg ccagactgac aagtcgggtg cgttcccgtc agggcctaag gtcccaccac
     1861 cacgaccgaa cggaggcagg gtgacagacg aactgagttt tcccgacgtc tcccgttccg
     1921 aactcgaccg cacactaagc cagctggtcg agaatgcgca ggacgtactc aacagtcagg
     1981 atcggctgcg cagtctgctc aaggcgaccc gggtcgttgc cggggagctc gagctggccg
     2041 aggttctgaa gcgcatcatc gaggccgcgg tcgaactcgt cggtgcacgc ttcggcgcga
     2101 tcggcgtgat cgcccccgat ggcacgctcg agcagttcat ccatgtcggg ctgcccgacg
     2161 aactggtcgg tccgatcggc cgcctgcccg agggccatgg tctgctcggt gccctcatcg
     2221 acgagcagga gcccatccgc ctcgaccacc tggccgagga ccctcgctcc tccggctttc
     2281 cccccggcca tcccgcaatg gacagctttc tgggcgtgcc cgtgcgggtg cgtgacgtgg
     2341 tctacggcaa cctctacctg tcggagagca ctgcgggctc cttcagtgcc gaggatcagg
     2401 agcttctcgt cgcgctcgct gccaccgcgg gcggggcgat cgatcacgcc cgcctgttcg
     2461 atgagacccg tcgtcggcag cggtgggcgt ccgctctcgc cgaggtgacc gcggcactgc
     2521 tctccgacga cgtgcaggac tccctcggta tcgtcgccga gcggatcacg acgctggccg
     2581 aagccgacct cgtcaccttc ctcggggcag cgtcgcccac gacgctgagg gtcgagctcg
     2641 cccgcggccc gctcgctccc ggcgtggagg gtctggtgtt cgatgctcag ggcaccgttg
     2701 ccggccgcgc gcaggagagc gggcagccgg tgctgtcgga tgctgctgcg gccagcgccc
     2761 tcggcgacgg ggtgcggctc ggacccacaa tggcgatccc gctcatcacc tccgacggcc
     2821 cgaacggcgt gctcaccgtc tcgcgggaac ccggacggtc gcgcttcacc gaggtcgacc
     2881 tcggaacggc ggctgacttc gccaggcagg ccagcgtggc gcttcgtctc gctgcaggtc
     2941 gtgcagaccg ccttcgcctg gcgaccctcg aagaccgcag tcgcatcgcg cgcgacctgc
     3001 acgaccacgt gattcagcgg ctcttcggcg ccgggctgag cctgcaggcg ctggcgggat
     3061 cgcttccgga tgaggcatcc cgggcgaaac tggtcgacca ggtggatgcg ctcgacgcgg
     3121 cgatcgcgga gatccgcacc gccatcttca ccatgaccac accccaggac gccgagcggc
     3181 cgtcgctgcg ccaccgggtc atcgacatcc tcggggagtc cgacggcctg ttcgagcagc
     3241 cccccgtgct gagcttctcg ggcgcggtcg acaccatgat cgatgtcgca ctcgccgacg
     3301 acgtcgccgc cgtggtgcgc gaaggactca gcaacgtcgc ccgccacgcc gcggcgcaca
     3361 acgtcaccat cgccgtcggc ttcgccgcgg gcagtgtcac cgtgagaatc gacgatgatg
     3421 gcaccgggat cgacccttcc gcggcagtgc gggcgagcgg caccgccaac ctcgcccagc
     3481 gtgccgaggc tcgtggtggt agcttcacgc tcgagccccg cgaaccgcga ggcacaacac
     3541 tgacctggat cgcacgagtg gagtcaaccg aatgaagcgc gtattcctgg tcgacgacca
     3601 cgagatcgtg cgtcgcggcg tcgccgacct catcaacgcc gaggccgacc tgactgtcgt
     3661 gggcgaggca tccacggtca agcaggcgct ctcgcgcatc gctgcgacga cccccgacgt
     3721 ggcggtgctg gatgtgcgcc tgcctgacgg atccggcatc gacctgtgcc gcgacatccg
     3781 ctcggcgcac ccggatgtcg cctgcctgat gctcaccgcg tacgacgacg acgaggccag
     3841 ctacaccgcc gtgctcgcgg gcgccgccgg ctacgtgctc aaggacatcc gcgggcaggg
     3901 tctgctggag agcatccgcc gcgtcgccaa tggcgagatc ctcgttgacc ccgcggtcac
     3961 caagcgcgtc gtcgcgcgcg ccaccaccgg tgcggatgcc gcggccgacc tgagcctgcg
     4021 cgaacgccag gtgctcgacc agatcgccgc gggactcacc aaccgccaga tcgcggagca
     4081 cctgggactc gccgagaaga cggtgaagaa ctacgtttcg ggcctgctcg cgaagctcgg
     4141 gatggagcgt cgcacgcaag ccgctgtgta ccgggcgaac cagacgccgt agcctcgaac
     4201 catgacgtcg atcaccagca tcccgcagaa gcccggcagg gtctggggag tgcttgcgct
     4261 catcgcggtg ctcttcccgc tgccgtggct gttcgtactc aacgtgctct cggtcgtggt
     4321 gcgctcgcag ccgccgcgca cgggcacgag cgccgaggag tgggcctacg gtctgatcgc
     4381 cttcggtggc ctgttcttct ttccgctgtt cgcgattctc acggtcttct tcgccatccg
     4441 cgcggtgctg aagccgcggg tcgcgggcaa ggtcatgggt tggattgcga tagccctcct
     4501 cgtgctgtcg atccctctga tctggaacgg gtacgcggtc tggatcatgg cgggtgcccg
     4561 atgagcgact tcgatccgcg cttcgacccc gcgttccagc gcggctatga cgggcccgcc
     4621 gtcacgcctc ccgcgacaac ggatgccccg gctcccgccg cgccgccgct cgtgcgcatc
     4681 gacccgcagg cagcggtcgc gccgcaggcc gagcaggatg agctgccgcc ccgcagaggc
     4741 aacccgttcc tcgtcgcact cgcggtcatc ggcgtcgcgc tggcggtcgg cggactggtg
     4801 ctggtgttcc agttgcgcac gatcttcgga gacggcaacg gcaaccccga cttcgactac
     4861 gtgaccctgc agacgctcat gatcgcagcc ccgattctga ccggcctggg agtggcgacg
     4921 ggaatcggtg tgctgttcgt gttcgcggtg cgctggggtc gctagggnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnngtg gaacggactg gaaagcttct cactgatgcc
     5101 tgtcaaccga acgggcacgg tggcgcgtcg agggggtgtg gctgtggcgg tgagcgagag
     5161 ggtccagctg gacctcgacg ccttcgtcgc gtccgagtac ccgaaggtcg tcgcggcagt
     5221 cgggctcatc acgggcaacc ggcaggacgc ggccgatgcc gtgcaggacg cgatcgtggg
     5281 cttccttgcg aagccgccga ctcatccggt caccaatctc gcggcctggt tcaccgtggt
     5341 cgcgagcaac cgggtcaagg acggctatcg cagcgaggcc gcccggcagc gcgcggtcgc
     5401 caagctcggg atgcccgtgg aggagaccac cgacgcgatc gagctgctcg acgtggatgt
     5461 ggccaacgcg ctcaagtcat tgccgttgca gcagcgccag gtgtgcgcac tgcactacct
     5521 gatggaccag tcgatcgaga cgatcgccga aggactcggt gtctcagaag gcaccgtgaa
     5581 gacccagctg caccgggccc gcaaggcgct cgccgcacgt ctgaggaagg aggatcacca
     5641 tggctgacat cgatgttctg ctgagcgctt cgctcaagcg catcgcccaa ccgggtgacg
     5701 ccgccggtgt tgccgatgcc atccgtgcgc gggtggacgc cggtgacacc ggcactcctg
     5761 ccaccgcaag cggcttcggc gcgcgcccgt ggtggctgtt cctgctgtca ggcctgctcg
     5821 tgatcctcgt ggcggtcacg ggcgtgtccg tcgccctcgg ccaaccgagc cccgtgacgc
     5881 ccactccgga tgccgcgtcc gtcgccgtct cgtcgagccc caccagctcg cccactctga
     5941 cacccacgcc cacggccacc ccgacggcga cggccgcacc cgagcccgct cccgcgcctg
     6001 ccgagcccga cccggagccc gccccgcccg ccgacacgac ggcaccggtg gtgactgtgc
     6061 ccacctcaag tcagccccaa ctcttctcgg ccaacggcgg gtcgacaatc atcacagcgc
     6121 tagccaccga cgacaccggg gtggtcgcgg tcgccatctc ctggacgggt gtggcgagcg
     6181 gcagtgcctc gatggtgccg ggctggagtt acaccttcgt gctgccaccc gcgtccggcc
     6241 cgccccccag cggcagcatc accttcaccg tgcaggcgtt cgacgcggcc ggcaacgcga
     6301 gcgacccggt atctgtggtg gtgccggtgc tgccctaact gcacgccccc cacagcccgg
     6361 catgccgtcg gaaggcctgc tcggtggcct gggctatggc ggagaagtgc gattcgttgt
     6421 cgtaggagga gaccgccgtc acgaagccct cgtcggcgag ggcgaggttc acgaactcac
     6481 cgttcgaggt ccagacgtac atcagccagc gtccgttgtc gtctttggcc gtcgcatccg
     6541 tcgccaccca ggccagggaa cccttgggca gcagctggcg caatcgaccc tgcgactcca
     6601 cggcgtagca ctcgttgatg agtccgaagt gcggtgcgta gacgccgatc aggcgcacgc
     6661 tgacagcacc ccaatcgggc acctgcggtc ctgggcgggg gttgctgagc acgacggtgt
     6721 cgcccgacag cacctcgcgc acgatcatgg gctgggcatc ggcgggtatc ggtgtcacgg
     6781 atgccgcgct cggagtactc tgcgcagacg caaccaccac gcccacaacc agtgccgcga
     6841 cgactccgag agccccaccg accaccgcac gagccaccac ggtcaggcct ccggccactg
     6901 gcacggtgac tgtgcgctct cgtgccagcc ccacagaccc tggccccgcc ggtaggcctc
     6961 ctccgtcgcc tgcgcgatca gcggatacag cggcccccgc tcggagaagt cggacgccct
     7021 ggccagcccc tgctcggcca gggtctcgtt gacgaagcgg ccttcggagt cccacacgta
     7081 catcgaccag atgccgcccg agtcgccctg ttcgttggtg gtcacccacg cgagcgagcc
     7141 ctcgggcagc atcgcgccga gcccgccctg cgcctcggcg gcgaagcact cgacgacggg
     7201 tgcgagatcg ggagcgtcga gtccgatcag ccgcaccgtg agatcacccc acagttgcac
     7261 ctgggcaccg gggcgagcgc tcgtcagcac gactgtgtcg cccgacagca cagtgcgcac
     7321 gatcatcggc tgggcatccg ctggccgcga tgctccggat gccccgtgtg cagtcggctg
     7381 cgtccacgca ctcgacaggg cggccacgat cgccgcggcg cctaccacgc cgcccacgac
     7441 ggcgcggacg accatggtcg cgaccctact ccggtcggtc ggcggggaac agtgccgctg
     7501 cggcgaaccc gctgcccggg cagcgctggg gtcagcggca tgccccgcgc attccgccga
     7561 acgagacgag cgcctgatgc tcaccgtcgg caaggatctg cccgtaccgg gtgtctgctg
     7621 ctgaatccgg cacctcggcg tatccgccca ccgccatcag gtagttcacg agccgtccgt
     7681 cggaacccca cacgttcagc agccagtcgc cggacgggtc gcgggtgggg gcatccgtcg
     7741 tcacccagac gatcgatccg gcgggcagca ggtcctcgag acgggcgagc gcttcggggg
     7801 cgaagcactg atcgacgtcg gttccgaatc gccgaccgag atcgggtgcg tcgagaccga
     7861 cgagacgcgc cgtcagccgg ccgaacaggg gcacctgagc accggcgcgg gtgcttgtga
     7921 gtacgacggt gtcgcccgac agcacctcgt ccacgagcat cggctgagcg tcgggcggct
     7981 gagcgaccgg ggattcgacg gattccgctc cgcgcgccca caggctcacg atcgcccagg
     8041 tgagcaggcc gagcgccagc accaccccga gtgtcacgcg cacgaccacg atcgcgaatc
     8101 tatcctcgtc agtccgcggg gaacagtacc gaggcgtcga gcccgccgcc cggccgcggc
     8161 tccagcttcg cgctgccgcg gctcgcttcg gcgagctggg cgacgatcgc gaggcccagg
     8221 ccgctgccgc cgacctgcga gctggcccgc cagaagcggt cgaaggcgcg agcgcaatcc
     8281 tccagcgaga gcccggggcc ctgatcgagc acgtgaagcg cgacgcggtc acccctcgac
     8341 tcgacccgca cgacgatcgt cgttccccgc ggactgaccg agagtgcgtt gtcgatgaag
     8401 ttgtccagaa tctgctcggt cgccgtcggc acgacgctgg ccacggcctg cgtcgccccc
     8461 tcgtagcgga tcgccacgcc cgactcatcg gagaggggca gccagtggtc gacgcgggcg
     8521 cgggcggcgg ccgcgacatc caccggcacc gcgacggccg acttggcctc ggtgcggctc
     8581 agcagcagca gcccctcgat gatggtgccg agccggtcgg cctcgctctc ggcggcggcg
     8641 agacgttcgg ccgcaccgtc ggggtcggtg tggatgaggt cgtgtgcgcg ctccagcttc
     8701 aggcgcaggg cggtgagcgg tgtgcgaagt tggtgcgagg catccgcggc gaaggagcgc
     8761 tgctgggcga tcagtccgtc gaggcgctcc gccatgacgt tgaacgagcg cgacagcgac
     8821 cgcagctctg acgccccggc gccctcgtcg gcgcgctcgt cgaggtgacc gtcggcgagc
     8881 cgctcggtgg atgcccgcag catcttcagc cggcgcgtca cccccgccgc caccacgtat
     8941 ccgaccacgc cggccaacag cacggtcgtg agcgccacga cccacagttg ccgcagctgg
     9001 gtggcgacag catcgtcgac gacctgggcg gggtaggtca gtcgcacggc accgaggatc
     9061 cgactgccgc tgaagaccgg caccgtcacg tagagcagct cctgctggag cgtctgggag
     9121 tagcgcgttc ccgaggcgat ctccccggtg agcgcggtgc cgatctcggg acgggagagg
     9181 taggaggcac cgatcttcga gtcgtcgggg tcgctggtga cgaccgcgat ccccttcccg
     9241 tcgacgacga tcacgcgcgc gccgctgctg gcctggtagc gcttggcgac ctcggtgatg
     9301 acggcgatcg actcgggcgt cggtgactcg agggcctcct cactgcgccc ggcgagcacg
     9361 aaggcgtccc gctccaggcc cgtgatgatc cggtcgcgct gcacgccctg gatgtagtag
     9421 ctcaggggga tgtcctgaac gaggatcacc agaagcgtgg tcgcgatcag ggcggccacc
     9481 agacgccact tcacggagca cccgaggccg tcactgggcg cccggctccc cgaaccggaa
     9541 gcccaccccg cgcaccgact cgatccagcc cgggtcgccg agcttcttgc gaatcgccgc
     9601 gatgtgggcg tcgagggtct tcgtcgttcc gtaccagttc gtgtcccaca cgtcgtggag
     9661 gatttcgctg cggcggaaca cggcgccggg atcggcgctc aagtacacca gcagctcgaa
     9721 ctccttggcg gtgaggtgca ccggctcgcc ggcgagggtg accttctggc tgcgggtgtc
     9781 caccacgagc gcaccgaagg tgcgcgccgc cgcgggctcg gcgacggtcg ggccggtgcg
     9841 cctcgcgacc gcacggatgc gggcgaccag ttctcgcatc ccgaacggct tcacgacgta
     9901 gtcgtcggcg cccatctcga gggcgacgac ccggtcgatc tcgtcgtcgc gcgcgctgac
     9961 cacgatgatc ggggtctgcg acaccgcgcg gatgcgccgg cacacctccg tgccgtccat
    10021 gtcgggcagg ccgaggtcga gcagcacaag gtcaggttgg tagctggaca gcgcgtcgag
    10081 tccaccctga ccggtgctca cgtggagtgc atcgacgttg gcctgcgcca gcccgtcgag
    10141 gatgccgtcg gcgactgacg cgtcatcctc cacgaccagt acccgcatgg ctcaattgtg
    10201 tcgcgggcgc gggcagtcgg agtacaccgc ggagatcttg gacagatctt ggatattcgt
    10261 cggtcacccc ttggatggcc tgatcgtagg ttggggacat gaagacctca gcaatcatcg
    10321 ccctctccat cgtgggagtc ctcggcacgg ccggggcggc catggccgtg aacggctcca
    10381 ccctcgcctc cttcagcgag ggcaccatcg gtcaggcgac cgatgtgctc gtccccagct
    10441 cgaccccgac ggatgacccc acgggcatcc cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnncccgag ccccagccac accgaggacg acgacagcga acacgagtcc
    10621 ggggacgatg actaagtcgc accccgcagg cggcacgcgc acgctggtcg gtctgatctc
    10681 gaccttcgcc ctcgtgggcg gggtcgtcgg attccagaat gcggcctcgg ccgaggaggc
    10741 tgctgtgcag cagcaggccc tcctcggtcg cggcgtcggc gtcgcatccg ctcgagtagc
    10801 acccaccgag gctcccgttg cggaggccgc tccggctgac tctgccccgg cctccgaccc
    10861 ggtgcccgcg gcagatcctg ctcccgcggc ggcgcccgcg cccgtggtgg atccggcacc
    10921 ggctcccgct ccagctcccg caccccccag cggcaccacg ggtgggtccg gtggataggt
    10981 ggagctcgac gcgatagctc gcggggcgct cctggcagcg cacgcacaga acgactccca
    11041 gcctccgacc tactccgatc gtcgacccct catgggcggc cacgccacca tcacgctggt
    11101 gggaggaggc gaggacctcg tcgcacgctg cttcgcgctc gccgaccacc tcgaggctcg
    11161 gtggagccgg ttcctgccca cgagcgacat cagtcggctc aactgggccg agggcgcttc
    11221 ggtggaggtc gacccggtca cggtgcagct catctccgcg atgcgcgagg cgtcggaact
    11281 cagcgacggt gacttcgacc ccacgctgct gccggagctg atcgcatccg gctacgcggc
    11341 ctcggtcgtg gcgcccgacc tcgtgaccac cctgccggcc tcggcgtcgg caccggggcg
    11401 ccttgcctcc ctcacacttg acggatcatc ggtgcggatg cccgtcggaa cgaccctcga
    11461 ccccggcggc atcgggaaag gtctcgccgc cgacatcatc tgcgagttcg ggctcgccga
    11521 aggtgcctgg ggcatcatgg ccgagatcag tggtgacatc gtcgtcgctg gccgggcacc
    11581 cgacgcggtg gcctggctgc tcggggtcga ggatccattc gacaccgatc gacacctgac
    11641 tactgtgcgc atcgcacgag gggcgatcgt gacgtcgagc cagcgcaagc gacgtttcga
    11701 cactgccgca ggtgttcggc atcatctgat cgacccgcgc acgggagcga gcgcgctgac
    11761 cgacatccag acggtgacgg tcatcgcagc aaccggcgtg cgtgccgagg ccatcagcaa
    11821 gtcggggttc ctgcgcccgg tcgaggagta cctggcctgg ctgccgcagg tcggggccgc
    11881 tgcgcttctc gtggattccg cggggcgcca gcatgtgtca gcaaactggg ggagatacgc
    11941 gtgagcctga acgatccgca catctggtgg tacgtcaccc gtgccagcgc cctgatcgcg
    12001 tgggcgctca tgacgctttc ggtggtgtgg ggcatcctgt tgtccacgcg cattctgcgt
    12061 cgcgtcgaca accccggctg gctgcaggac ctccaccggt tcctcggcgg actgtccgtg
    12121 atcatggtgc ttctgcacat ggtcacgctc atgctcgacg gctggctgca cctgagcctg
    12181 gtgcaggtgc tcgtgccctt caccagtgac ttcaagccga tcgctgtcgc actcggcatt
    12241 gtcgccttct acctgctcat cgcggtgcag ggctcctcga tgatcatgca tcgactgccg
    12301 cgcacgttct ggaagggtct gcactacgcc agttacgcgg tcgtgctgct ggtgtcgttc
    12361 cacgccgggc tctccggcac ggatgtcggc tcgctcggct acaagattct cgcgttcagt
    12421 ctcatcggcc tcaccgccgc cgcgaccgtc gtgaggatcg ccgtgaccaa ccgcacgacc
    12481 cgggcggcga aggccgaggt gtgggtgcct ccgcacgacc cggcgccgcg catctcaccg
    12541 ccgcggatcc agacccgcac gatggtggtc gctgactccg ggttgatcgc cgagggggtc
    12601 atgggcatcc gcctcgtcgc cctcgggggc gggctgttgc cgatctggta ccccggcgcc
    12661 cacgtcacac tgcacctggc caatggtctc gagcggcagt actcgctgtg tggcgacccg
    12721 ggcgagcgac accactacga cctcgccgtt ctgcgcaccg aggactcggc cggcggcagc
    12781 cgctgggtgc acgagagcgt ggtgcccggt acgaccatcg aggtctcggg tccgctgaat
    12841 cacttcgagc tcgagccatc gcgggagtac ctgttcatcg cgggaggcat cggcatcacg
    12901 cccatcaagg cgatgatcga gtccctcccg gcgatccggg agtggcgact catctatgtc
    12961 ggtcgaagtc gtcgcagcat ggcgttcgcc aacgaactgg ccgagcgcta cccggaccgg
    13021 gtgcgcatcc acgcgagcga tgagagcccg gcccagctcg acttcacccg actcgtgaca
    13081 cccgacacca ccgaggtgta ctgctgcggg cccgagtccc tcatgtccgc cgtggcctcg
    13141 gcggtgccgt cggcgcgact gcacgccgag cggttcattc cgatcgaacg ggtgccgagc
    13201 atcccggacc agccgctgcg cctcaccttc agccgcagcc cgttggcgct caccgtgggg
    13261 gccggcgaga cggtgctcga cgcactcgaa cgcggcggga tgcccgtgag cgggtcatgc
    13321 cgcaaggggg tctgcggcac gtgcgaggtg aaggtgctgg cgggcacccc cgagcacctg
    13381 gactcggtga tggacgacgc cgagaaggac aagctgaaga tcatgtaccc gtgcgtgtcg
    13441 cgggcgctga cgccggagct ggtgctcgac ttctagctgc cggtggtcga gcctgtcgag
    13501 acctaccggt ggtcgagcct gtcgagaccc cctacacctg gcagttcggg caccaccaca
    13561 cgtcgcgctg cgtgagtgcg gtgtcgccga gctcgccgtg ctggatgggc gtgccgcagc
    13621 gcaggcacgg cttgccggtg cgcccgtaga cccagaggcg ctgaccgttc agctggccgg
    13681 tcgtcgtgcg ctgggcgcga tccttgttgg cgtcgatgag ccgccaggcg agctcgacca
    13741 tcttctcgag ctcgggcacc tcgccgacgg gccggcgcgg aagcaccccg cgcagaaagc
    13801 acagctcggc gcggtacacg ttgccgagtc cggcgaggtt gcgctgatcg agcagggcga
    13861 gtccgatctc gcgctcgggg ttcgcgcgca ggttggcgag agcctgctcg ggctgccagt
    13921 cgccgaggag gtcggggccg aggtatccga ccacgctgtc ttcgtcgtcg cggcgcacga
    13981 cctcgaactc gcccaggtcg aagcccaccg cgaggaactc tgcggtgccg aggatcgcgc
    14041 gcgcctgaaa ctcggggcgg aaccacttcg acccgtgcct gtagaggtgc caggagccct
    14101 ccatcttgag gtgcgagtgc agggtatatt ccccgacgtg catgagcagg tgcttgccgc
    14161 ggctcgccac gccctcgatc gtctcgcccg tgagatcgac ggtcgcgaat gcgggcaccc
    14221 ggatgtcgca gcgcgtcagc acctgccccg cgagggccgc gttcagattc ttcgccgagc
    14281 ggtagacggt gtcgccctca ggcattgttc tcgagcctcc cgtcgatcgc tcgatccgcg
    14341 ctggctttcg tcttgcgacg acgatcccct ccgactactc ccgttgtgca ggagacgcga
    14401 gccccgctcg gcgatcctgc ataacagaag tgtcgagcag cagactgccg cgttgggcgg
    14461 tcgcacctac gcattgcgca acctcagccc ctgcggcgtc gccacgaagc cggcctcgac
    14521 gagggcgagg cccagggggg tgccgaccgc gaattccccg tcgatgcgct ccaccttgag
    14581 cttgccgagc gcggtgcgca cggtcgaggt gagactgcgg gatgccgcgg cgagggcatc
    14641 cgcgtcagaa ccgaaggtga gcaccgtctt gccgccgcgc tcgacgtaga gcgtgagggc
    14701 tccgtccacg agcacgacga gggcgcccgc
//
LOCUS       DAOSOU010000026        14661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4132_length_14661_cov_7.158428, whole genome shotgun sequence.
ACCESSION   DAOSOU010000026 DAOSOU010000000
VERSION     DAOSOU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 14661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14661
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4132_length_14661_cov_7.158428"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..665
                     /gene="rpsF"
                     /locus_tag="PLW15_02515"
     CDS             180..665
                     /gene="rpsF"
                     /locus_tag="PLW15_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT33304.1"
                     /translation="MTHQYELMVILDPEIDERTVAPSLDKFLNVIRTDGGTIDNVDIW
                     GRRRLAYEINKKTEGIYAVVNFTATSTATVELDRQLKLSEAVMRTKVLRAEQGFAQVA
                     AAKKLSDEKAARKAAAPAKAPAAAAAPAAAPAAKVETEDAPAKPAAAAKPKAAPAQDA
                     E"
     gene            669..1169
                     /locus_tag="PLW15_02520"
     CDS             669..1169
                     /locus_tag="PLW15_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT33305.1"
                     /translation="MAGETVITVVGNLTSDPELRYTQNGLAVANFTIASTPRNFDRAS
                     NDWKDGEALFLRASVWREFAEHVAGSLTKGSRVIATGRLKQRSYETKEGEKRTSIELE
                     VDEIGPSLRYATAQVTRAASSRDSSGGAPRGGGQVTEEPWAASAPDAGGDVWNTPGNY
                     TDETPF"
     gene            1250..1513
                     /gene="rpsR"
                     /locus_tag="PLW15_02525"
     CDS             1250..1513
                     /gene="rpsR"
                     /locus_tag="PLW15_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOT33306.1"
                     /translation="MAGKSSGDRRKPIRKGKDGKNAAPAKAIRVGVIDYKDVATLRKF
                     ISERGKIRARRITGVSVQEQRLIATAVKNAREMALLPYAGSGR"
     gene            1526..1978
                     /gene="rplI"
                     /locus_tag="PLW15_02530"
     CDS             1526..1978
                     /gene="rplI"
                     /locus_tag="PLW15_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT33307.1"
                     /translation="MSKLILTHEVTGLGAPGDVVDVKNGFARNYLIPQGFAVAWTRGG
                     EKQIESIKAARAAREHATLEEAQDLKARLQAATVSLTVKAGEGGRLFGSVKTSDIADA
                     VAAAGLGSIDKRKIEITSPIKATGEHEATVRLRDDLVATITLKVVAAK"
     gene            2392..3768
                     /gene="dnaB"
                     /locus_tag="PLW15_02535"
     CDS             2392..3768
                     /gene="dnaB"
                     /locus_tag="PLW15_02535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT33308.1"
                     /translation="MSIAHLGLAGDQRQSSDSRGDRVPPHDLLAEQSAIGGMLLSKDA
                     VADVIETVRGMDFYIPKHETIFEAVMNLYSHGEPTDVIAVTDELTKTGELTRAGGAEY
                     LHTLTALVPTAANAGFYASIVAEKAVLRRLVEAGTRIVQMGYASEGEVVDLVNNAQAE
                     IYAVTGGVESEDFVPLTEAVTTAIDEIETAMGRDGKMVGVPTGFAELDDLTNGLHPGQ
                     LIILAARPALGKSTLGLDFARSAAIKNDMPTIIFSLEMGRSEIAMRLLSAEASVPLQH
                     MRKGTVHTNDWTTIASTRGRINDAPLYIDDSPNMTLVEIRAKCRRLKQRVGLKMVIID
                     YLQLMTSGKKVESRQQEVSEFSRALKLLAKELSVPVIAISQLNRGPEQRADKKPALSD
                     LRESGSLEQDADMVILLHRESAYEAENPSAGTADFIVAKHRNGPTRTITVGFHGHFSR
                     FVDLPPGS"
     gene            3779..4369
                     /locus_tag="PLW15_02540"
     CDS             3779..4369
                     /locus_tag="PLW15_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33309.1"
                     /translation="MTSWYVPLARAIPALVLAAVVTFSADHSPALGLITFGIFAAATG
                     AVITAFALRSPAGTARVLQLTQGIVTLVAGIVSLAMIAGGLPFFVLVLTAFAVVTGFL
                     ELYLGLRSRRTDRSARDWIFTGGLTVLLAIIVLLVPPGFVQTFEGPDGVVRELTASVI
                     VVGTLGAYWAILGIYLVIAALSLKWAPDDTATPKAA"
     gene            4371..4640
                     /locus_tag="PLW15_02545"
     CDS             4371..4640
                     /locus_tag="PLW15_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33310.1"
                     /translation="MSTPPTPPSRRDRTRPVELLLISGVLAIFIGLVVLMSTRQVVLA
                     LIFLGVSFIVALVVLAMLALAVRPTGDEQLDIDEQDRDQGGRRGH"
     gene            complement(4681..6063)
                     /gene="purB"
                     /locus_tag="PLW15_02550"
     CDS             complement(4681..6063)
                     /gene="purB"
                     /locus_tag="PLW15_02550"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT33311.1"
                     /translation="MSLPEQPLSPLDGRYRPAVSALGEHLSEAGLNRARVRVEVEWLL
                     YLTDHELFGSVKLDHEQARQLREFAAEFGQAEIDELAELEATTRHDVKAVEYLVRRKL
                     SDLGLTGISELTHFACTSEDINNLSYALTISAAVREVWLPKFRSVIAALRERAIELRD
                     DAMLAHTHGQPATPTTMGKELAVFVYRLDRILAQIEATEYLGKFSGATGTFAAHLAAD
                     ATQDWPAISSEFVTSLGLTWNPLTTQIESHDWQAELYSRISHANRVLHNLCTDVWTYI
                     SMGYFRQIPQPGATGSSTMPHKINPIRFENAEANLELSSALLDSLAATLVTSRLQRDL
                     TDSTTQRNIGVALGHSLLALDNIERGLKEIDLNRDALAADLDANWEILGEAIQTVIRA
                     EVTAGRSSIADPYAILKELTRGKRVGQAELVEFIDGLDIGADAKARLRELTPATYAGL
                     ASELVEYLAG"
     gene            complement(6060..6584)
                     /locus_tag="PLW15_02555"
     CDS             complement(6060..6584)
                     /locus_tag="PLW15_02555"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOT33312.1"
                     /translation="MSFERTFDESALFRICFVCTGNICRSPMAETIFKSMVKKAGYEN
                     RIAVISAGTGDWHVGEPSDERTLAALAAQGYNGSGHRGRQFDPDWFDKLDLVVAFDRS
                     QERILKAWAPTDQDRAKVQLLLSFDSDQAHLVDVPDPYYSDAALFDQVLVMIQKACAA
                     LFRQITPGIRQGAP"
     gene            6696..6956
                     /locus_tag="PLW15_02560"
     CDS             6696..6956
                     /locus_tag="PLW15_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33313.1"
                     /translation="MTLPTDATLLAALETQYRRLTELLQRLEYARATLVPPPANFWIG
                     AARLAYDSGIDAMAGTVDGAVLAMRSAIQFTASAVAEVSARG"
     gene            6949..8325
                     /locus_tag="PLW15_02565"
     CDS             6949..8325
                     /locus_tag="PLW15_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33314.1"
                     /translation="MGDLVVSGGATTAVAPEELERAGVLLRRLSAEASSIAMELASID
                     RIATRETLREMGAPTSALEAENDIDQATIVLGEVTVIADLHGQALQFAADGYTTVEQF
                     VRVMFGSGMGDLASAGGALSRLGPLGLILAGGAAAAASNPQLRGHPFFVNAVRESVMS
                     SDDALLGYLGVPRVVSRALGDEGLGLTGASTTAAVLAGLGGAVGLVRHTPVRLTAESV
                     PTEVRPVEGAEGRLDRVPTPTDGDGAQITIERYEYEGKPDAFIAYVAGTATFDPFVTD
                     EPLDMESNVLNMVDGSSASAEALRQALAAAGADASSQVQLVGYSQGGAADARVAESGD
                     FDVQGILTFGSPTGQIELPTDITAILVEHDDDLVPATGGRQDNQSALLVNRRVYDDWQ
                     VPADQPVPAHLRSNYAETARMMDASTGELRSTIDALDSLTQGATRVTSTNYEFERITG
                     SASVSGRS"
     gene            complement(8309..8710)
                     /locus_tag="PLW15_02570"
     CDS             complement(8309..8710)
                     /locus_tag="PLW15_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT33315.1"
                     /translation="MRLFVKLLINSLALWLTTLIVAGVHVVPFAPGGTLEIVLTYLLV
                     AAIFGIVNAIVGNFIRIVAFPIYILTLGLISLIVNGLLLLLAAWLSSLFGFGLSVDSF
                     WWGVLGALVLGLISWLIGILLRPIIGSKNGR"
     gene            8796..9887
                     /locus_tag="PLW15_02575"
     CDS             8796..9887
                     /locus_tag="PLW15_02575"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT33316.1"
                     /translation="MTKPRVKLRPEVEQMVPYRQGRAAADDAFKLSSNENPFEPLESV
                     LEAIAESSINRYPEGSAAALRDRLGARYGVSPERIQVGAGSVSVLSQIISAASAAGDE
                     IVYSWRSFEAYPLLVATAGATSVQVPNTADHRHDLPAMLAAITDRTRLVIVCSPNNPT
                     STVVTDDEFRAFMAEVPSTVLVVLDEAYAEFVTDAEAVRGESVIHDYPNLVVLRTFSK
                     AYGLAGLRIGYAVGEEYVMDAARAVAIPLSVTEPAQRAAIVSLAHEPELLQRVAHLAT
                     LRDRIWEGLVEQGWDTPKPHGNFVWLPTGDDTPIAAGIFEAHGIVVRALGEGLRVSVG
                     ESESVDKLLRASAEVVKMRRTAPPTATLD"
     gene            9892..11010
                     /locus_tag="PLW15_02580"
     CDS             9892..11010
                     /locus_tag="PLW15_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOT33317.1"
                     /translation="MAYTAATVQLLSETGELVSSALSEPYLPIIEKLSDEQLREFHRQ
                     MVVFRAIDVEAANLQRQGQLALWIPSLGQEAAQVGSGFAAKPQDHIFPAYREHVVGRI
                     RGVDTIKIIEILRGLSHGGWDPAEHGNFHLYTLVIGSQTLHATGYAMGITLDGRAATG
                     DPDKDEAVIVYFGDGASSQGDVSEALVFAASFQTPEVFFLQNNHWAISVPVTRQSRTP
                     LYLRPSGFGIPGVQIDGNDVLASYAVTTASLDAARSGGGPQFIEALTYRMGAHTTSDD
                     PTKYRDDAELDFWVRHDPIVRFEAYLRSRGADDAFFAELAEEAHDAANDIRLRTLALG
                     VPDASSMFTHVYTEPHSLMQEQQAWLEAYEASFEGGAS"
     gene            11028..11987
                     /locus_tag="PLW15_02585"
     CDS             11028..11987
                     /locus_tag="PLW15_02585"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOT33318.1"
                     /translation="MAKALNNGLRKALADDPKVLLMGEDIGPLGGVFRVTEGLQAEFG
                     ANRVIDTPLAESGIVGTAIGLAMRGYRPVCEIQFDGFIFPGFDQITSQLAKLTARHEG
                     SLSMPVVIRVPFGGHIGAVEHHQESPEAYFAHTPGLRVVSPSTPHDAYWMIQEAIASN
                     DPILFFEPKSRYWPKGDVDTSAPGIPLHASRVVRVGTEVTLVGYGPMVPMLLQAAEIA
                     AEEGTSIEVVDLRSISPIDYGPLVASVQKTGRLVVAHEAPGFVSVGSEIAATVTERAF
                     YSLEAPVIRVAGFDTPFPPAKLEAAYLPDPDRILDAVDRALAY"
     gene            11997..13337
                     /locus_tag="PLW15_02590"
     CDS             11997..13337
                     /locus_tag="PLW15_02590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT33319.1"
                     /translation="MANSDFTLPDVGEGLTEAEIVSWKVKAGDTVTLNQVLVEIETAK
                     SLVELPSPFEGTVAALLVEEGQTVEVGTPIITVSSSGDAPAPAAPATPVVEAVADTAA
                     TVAAPEKPAEEKPGAVLVGYGSSGHGSSRRRRGAGPAATADATVAPAARPASVPAAAA
                     SPIIAKPPIRKLAKDLGVDLATVLATGLAGEITRDDVIRQASQASVFRNIETPEWGDQ
                     REERIPVKGVRKAIATAMVQSAFSAPHVSIFTDVDATRTMEFVKRLKNSPDFAGVRVS
                     PLLIMAKALIWAVRRNPNVNSSWTDKEIIVHHYVNLGIAAATPRGLIVPNVKDAHSLS
                     LIDLATALEQLTITARDGKTQPADMANGTITITNIGSYGMDTGTPIINPGEATIVALG
                     TIKQKPWVVDGEVRPRFVTTVGASFDHRIVDGDVASRFVADLASVLEEPALLLD"
     gene            13599..14456
                     /locus_tag="PLW15_02595"
     CDS             13599..14456
                     /locus_tag="PLW15_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOT33320.1"
                     /translation="MKLFALLAAAALLLTGCAATTPAADDTIRIVASTDVYGDFAQTI
                     GGDTVTVTSLIAGASRDPHSFEASAQDQLAVNKADVVIENGGGYDPFVETLLAASGST
                     ATVISVAELVNLPEGTNEHLWYDFGAMDSFAKKLADTLAALDPENAAEYASNYETLSG
                     RLDGLTQKVTGLADGQGVAVTEPVPLYLLEAAGLVNRTPADFTEAIEEGSDVPPAALQ
                     ETLALFGDGAVVLLAYNDQTASPETERVRAAAEAAGIPVVDFTETLPDGQTYLSWMTA
                     NIDALAAVL"
     gene            14453..>14661
                     /locus_tag="PLW15_02600"
     CDS             14453..>14661
                     /locus_tag="PLW15_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33321.1"
                     /translation="MTPALQITQGALGFGGRTLWRELDLSVRPGEFLAVLGSNGSGKT
                     SLLRAILGLQPLDTGTIQIDGMPARR"
BASE COUNT         2483 a         5017 c         4827 g         2334 t
ORIGIN      
        1 gagttggcac agttgccgac acccgcaagg gctacgcgca cagacatggg atggtgctcc
       61 tgacgggaaa tgactacact gggcaggttg tctggaaccg ttcgcggaac cacgacaatc
      121 gtcaacaccc tcctgttgca gatcgtctgc aaccgttcta gtccgaagga ggtgggtttg
      181 tgacgcacca gtatgaactc atggtgatcc tcgatccaga gatcgatgag cgcaccgtgg
      241 cacccagcct agacaagttc ctcaatgtca ttcgtaccga tgggggaacc atcgacaacg
      301 tggacatctg gggtcgtcgt cgtctggcct acgagatcaa caagaagacc gagggcatct
      361 acgccgtcgt caacttcacg gcgacctcga ccgctaccgt cgaactcgac cgccagctga
      421 agctcagcga agccgtcatg cgcaccaagg tgctgcgtgc cgagcagggc ttcgcgcagg
      481 tcgccgccgc caagaagctc tcggacgaga aggccgcccg caaggctgcc gctcccgcca
      541 aggctccggc cgctgccgcc gctccggctg ccgcgcccgc cgccaaggtc gagaccgagg
      601 atgcccccgc caagcccgcc gcggccgcga agccgaaggc tgcgcccgcc caggacgcgg
      661 agtaggtcat ggccggcgag accgtcatca ccgtcgtggg taacctcacg agcgacccgg
      721 agctgcgcta cacgcagaac gggctcgcgg tggcgaactt caccatcgca tcgacgccgc
      781 gcaacttcga ccgtgcgagc aacgactgga aggacggtga ggcactcttc ctgcgcgcga
      841 gcgtatggcg cgagttcgcc gagcacgtcg ccggttcgct cacgaaggga tcgcgcgtca
      901 tcgcgaccgg tcgtctgaag cagcgttcgt acgagacgaa ggaaggcgag aagcgcacga
      961 gcatcgagct cgaggtcgac gagatcggcc cgagcctgcg ctacgcgacc gcccaggtca
     1021 cccgtgccgc gtcatcccgc gattcgtcag gcggtgcccc ccggggcggc ggccaggtca
     1081 ccgaggagcc gtgggcggca tccgcccccg acgccggcgg cgacgtctgg aacacgcccg
     1141 gcaactacac cgacgagacg ccgttctagc tctcggattc ctgatctcga tacgcggctg
     1201 cgccgctcct cgatcaccga ttactacgta ctgaaggaaa tagaaactca tggcaggaaa
     1261 gagcagcggc gatcgccgca agcccatccg caagggcaag gacggcaaga acgccgcccc
     1321 ggccaaggcg atccgcgtgg gcgtcatcga ctacaaggat gtcgccacgc ttcgcaagtt
     1381 catctccgag cggggaaaga tccgcgctcg ccgcatcacc ggtgtctccg tgcaggagca
     1441 gcgtctcatc gccacggccg tcaagaacgc ccgtgagatg gcactgctgc cctacgccgg
     1501 ctcgggccgt taaggagtat cgacaatgtc caagctgatc ctcacgcacg aggtcaccgg
     1561 tctcggtgcc cccggcgacg tcgttgacgt caagaacggt ttcgctcgca actacctcat
     1621 cccccagggc ttcgccgtgg cgtggacccg cggtggcgag aagcagatcg aatccatcaa
     1681 ggccgcccgc gctgcgcgcg agcacgccac cctcgaagag gcgcaggacc tgaaggctcg
     1741 ccttcaggcc gccaccgtgt cgctgaccgt caaggccggc gaaggcgggc gtctgttcgg
     1801 ctcggtcaag acctcggaca tcgcggatgc ggttgctgcg gcgggtctcg gctcgatcga
     1861 caagcgcaag atcgagatca ccagcccgat caaggcaacg ggtgagcacg aggcgacggt
     1921 tcgccttcgt gacgacctcg tcgcgaccat caccctgaag gtcgttgccg cgaagtagag
     1981 catcaacgac ggatagcgac gagttgaacg ctcgtcgcta tccgtgttta acgagcaaaa
     2041 gtcaagcggt ttatgcacag gaatcgactt ttctcgacaa ccctcaagcg gcagtctaaa
     2101 catgtttcta cacacaccgt gtgaaacgta aaagtgcagg tcaggggggt gcaacggcaa
     2161 caaattggcg agttctccac agtaaagtgc acagcttgct cacacgacgc ccggcgtttc
     2221 tcgcactttt cccacacctt catccacagg gggtgttgtc gggcctgacg gcgcctccct
     2281 atggttgtcc agtcccgccg cctcgacggg actgggggag aagctggtgg ccggagtgtc
     2341 ggcggacgcc gctacaactg aggcgtagcg tgatccactg ggaggggtcg tatgtccatt
     2401 gctcacctgg gactcgctgg cgaccagcgc cagtcgagcg actcccgtgg cgatcgcgta
     2461 cccccgcacg atctgctggc agagcagagc gccatcggcg gcatgcttct gagcaaggat
     2521 gccgtcgcgg acgtcatcga gaccgtgcgc ggcatggatt tctacatccc caagcacgag
     2581 accatcttcg aagcggtgat gaacctctac tcccacgggg agccgacaga cgtcatcgcc
     2641 gtcaccgatg agctgaccaa gaccggcgaa ctcacacgag ccggcggcgc cgagtacctc
     2701 cacacgctga ccgcgctcgt gcccaccgcg gccaacgccg gtttctacgc gtcgatcgtc
     2761 gccgagaagg ccgtgctgcg tcgcctggtc gaagcgggca ctcgcatcgt gcagatggga
     2821 tacgccagcg agggcgaggt cgtcgacctc gtcaacaacg cccaggccga gatctacgcc
     2881 gtgaccggcg gcgtcgagag cgaggacttc gttccgctca ccgaggccgt caccaccgcg
     2941 atcgacgaga tcgagaccgc catgggccga gacggcaaga tggtcggcgt tcccaccggc
     3001 ttcgccgaac tcgacgatct cacgaacggt ctgcaccccg gccagctgat catcctcgcg
     3061 gcgcgccctg cgcttggtaa gtcgaccctc ggcctcgact tcgcccgctc ggcggccatc
     3121 aagaacgaca tgcccacgat catcttctcg ctggagatgg ggcgcagtga gatcgccatg
     3181 cgtctgctgt cggccgaggc atccgtgccc ctgcagcaca tgcgcaaggg caccgtgcac
     3241 accaacgact ggacgactat cgcgtcgacc cgcggccgca tcaacgacgc gcccctgtac
     3301 atcgacgaca gtcccaacat gaccctcgtc gagatccgcg cgaagtgccg acgcctcaag
     3361 cagcgcgtgg gcctgaagat ggtgatcatc gactacctgc agctcatgac gagcggcaag
     3421 aaggtcgagt cccgccagca ggaggtcagc gagttctcgc gtgcactgaa gctgctcgcc
     3481 aaggagctca gcgttccggt catcgcgatc tcacagctca accgtggtcc cgagcagcgc
     3541 gccgacaaga agcctgcgct atccgacctc cgtgaatccg gctccctcga gcaggacgcc
     3601 gacatggtca tcctgctgca ccgcgagagc gcgtacgagg cagagaaccc gagcgccggc
     3661 accgctgact tcatcgtcgc gaagcaccgc aacggcccca cccgcacgat caccgtgggc
     3721 ttccacggtc acttctcgcg gttcgtggac ctgccgccgg gttcgtagac tggagtccgt
     3781 gacttcctgg tacgtgccgc tggcgcgcgc gatccccgca ctcgtactcg cggcggtcgt
     3841 gaccttctcc gccgaccact cccccgcgct cggcctgatc acattcggca tcttcgccgc
     3901 ggccacgggc gccgtcatca cggcctttgc actcaggtcc ccagcaggca ctgcgcgagt
     3961 gctgcagctc acccagggca tcgtcacgct cgtcgccggc attgtgtcgc tggccatgat
     4021 cgcgggcggc ctgccgttct tcgtcctagt gctgaccgct ttcgcggtcg tgaccggatt
     4081 cctcgagctc tacctcggcc tgcgcagccg tcgcaccgac aggtccgccc gggactggat
     4141 cttcaccggc ggcctcaccg tgctgctggc gatcatcgtg ctgctcgtgc cgccggggtt
     4201 cgtgcagacg ttcgaggggc ccgacggcgt ggttcgtgag ctcacggcat ccgtcatcgt
     4261 cgtcggaaca ctgggcgcct actgggcgat cctcggcatc tatctcgtga tcgcggcgct
     4321 ctcgctcaag tgggcgccgg atgacacggc aaccccgaaa gcagcctgac atgtccacgc
     4381 cccccacccc gccgagccga cgcgatcgca cgcgtcctgt ggaactgctc ctcatctcgg
     4441 gagtgctcgc gatcttcatc gggctggtcg tgctcatgtc gacgcgccag gtcgtgctgg
     4501 cgctgatctt cctcggcgtc tcgttcatcg tcgcgctcgt cgttctggcg atgctggcgc
     4561 tcgccgtgcg cccgaccggc gacgagcagc tcgacatcga cgagcaggac cgcgaccagg
     4621 gtgggcgcag aggccactga gtctggcggg ttgagtagcc gcgacgcggc gtatcgaaac
     4681 ctagcccgcg aggtactcca ccagctcgct cgccagcccc gcataggtcg ccggcgtgag
     4741 ttcacggagc cgcgccttgg cgtcggcgcc gatgtcgaga ccgtcgatga actcgaccag
     4801 ttcagcctga ccgacccgct tgccgcgggt gagttccttg aggatcgcgt acgggtcggc
     4861 gatcgaactg cggcccgcgg tcacctccgc gcgaatgacg gtctggatcg cctcgccgag
     4921 aatctcccag ttggcgtcga ggtcggcggc cagggcatcg cggttcaggt cgatctcctt
     4981 gaggccgcgc tcgatgttgt cgagcgccag cagcgagtgt cccagcgcga ctccgatgtt
     5041 gcgctgcgtg gtggagtcgg tgaggtcgcg ctgaaggcgc gaggtcacga gcgtcgcggc
     5101 aagcgagtcg agcagcgcgc tggacagctc aaggttggcc tcggcgttct cgaagcggat
     5161 cggattgatc ttgtggggca tcgtcgacga cccggtcgcc cccggctgcg gtatctgccg
     5221 aaagtagccc atcgagatgt aggtccacac gtcggtgcag aggttgtgca gcacgcggtt
     5281 ggcgtggctg atgcgcgagt agagctccgc ctgccagtcg tgcgactcga tctgggtggt
     5341 gagcgggttc caggtcagac cgagggatgt cacgaactcg ctcgagatcg cgggccagtc
     5401 ctgcgtggca tccgccgcca ggtgcgcggc gaacgttccc gtcgcgccgc tgaacttgcc
     5461 gaggtactcc gtggcctcga tctgggcgag gatgcggtcc aggcggtaga cgaagaccgc
     5521 gagctccttg cccatggtcg tgggggtggc cggttggccg tgcgtgtggg cgagcatcgc
     5581 gtcatcccga agctcgatgg cccgctcgcg cagggcggcg atcacgctgc ggaacttcgg
     5641 cagccagacc tcgcgcaccg ccgcgctgat ggtcagcgcg taggagaggt tgttgatgtc
     5701 ctcgctcgtg cacgcgaaat gggtgagttc ggagataccc gtgaggccga ggtcgctgag
     5761 tttgcggcgc accaagtact cgacggcctt cacgtcgtgg cgcgtagtcg cctcgagctc
     5821 ggcgagctcg tcgatctcgg cctggccgaa ctcggcagcg aactcgcgca gctggcgagc
     5881 ctgctcgtga tccagcttga ccgagccgaa cagctcgtgg tcggtgaggt agagcagcca
     5941 ctcgacctcg acccgcacgc gggccctatt gaggccggcc tcggagaggt gctcccccag
     6001 cgcagagacc gcgggtcggt agcgtccgtc gagcgggctc agcggttgtt cgggcaggct
     6061 catggggctc cctgtcggat tcctggggtg atctggcgga agagtgcggc gcacgccttc
     6121 tggatcatga cgagcacctg atcgaagagg gcggcatccg aatagtaggg gtcgggcacg
     6181 tcgacgaggt gcgcctgatc gctgtcgaag ctcagcagca gctgcacctt cgccctgtcc
     6241 tggtcggtcg gggcccaggc cttgaggatt cgctcctggc ttcggtcgaa ggcaacgacc
     6301 aggtcgagct tgtcgaacca gtccgggtcg aactgtcggc cgcggtggcc cgagccgttg
     6361 tagccctggg ccgcgagcgc ggcgagtgtg cgctcatccg atggctcacc gacgtgccaa
     6421 tcccccgtgc cggcggagat cacggcgatg cggttctcgt agccggcctt cttgaccatc
     6481 gacttgaaga tggtctcggc catgggcgac cggcagatgt tgcccgtgca gacgaagcag
     6541 atgcgaaaga gcgccgactc atcgaaggtg cgctcgaagc tcatgcttca ttgtcccgca
     6601 gaaactcctc ctcaccacga cccgtctgac ggcacgtgcc cacgggtcga gcgactgcag
     6661 cgggcgggcg ctggctcggg gacgatcctc gttccatgac tcttccgacg gatgccaccc
     6721 tgctcgccgc gctcgagacc cagtaccgac gcctcaccga gcttctgcag cgcctcgagt
     6781 acgcgcgtgc gacgctcgtg cctccccctg ccaacttctg gatcggggcg gcccggctgg
     6841 cctacgactc cgggatcgat gcgatggcgg gcaccgtcga cggtgccgtg ctcgcgatga
     6901 ggtcggcgat tcagttcact gcgagcgccg tggcggaggt gagcgctcgt gggtgacctg
     6961 gtggtcagcg gtggtgcgac gacggcggtc gcgcccgagg aactcgagcg tgccggggtg
     7021 ctcctgcgcc ggctctccgc tgaggcctca tcgatcgcga tggagctggc gtcgatcgac
     7081 cgtatcgcca cccgggagac gctgcgggag atgggcgctc cgacaagcgc gctggaggcg
     7141 gagaacgaca tcgaccaggc cacgatcgtg ctgggcgagg tcaccgtgat cgccgacctg
     7201 cacggacagg ccctgcagtt cgcggcggac ggctacacga ccgtcgagca gttcgttcgg
     7261 gtgatgttcg ggtcgggaat gggcgacctc gcctcggcgg ggggcgcgct cagccggctc
     7321 gggccgctcg gcctcatcct cgccgggggt gcggcggccg cggcatccaa ccctcaactg
     7381 cggggtcacc cgttcttcgt gaacgcggtg cgcgagtcgg tgatgtcatc ggatgacgcg
     7441 ctgctcggtt acctcggagt cccgcgcgtc gtgtcgcgcg cgctgggcga tgaggggctc
     7501 ggtctgaccg gagcgagcac gaccgcggca gttctcgcgg gcctgggcgg cgcggtcggt
     7561 ctggtgcggc acactcccgt caggctgacg gccgagtctg tgccgaccga ggtgcgtccg
     7621 gtggagggcg cagaggggcg gctggatcgg gtcccgacgc cgaccgacgg cgacggtgcg
     7681 cagatcacga tcgagcgcta cgagtacgag ggcaagcccg atgcgttcat cgcctatgtc
     7741 gcaggcacgg caaccttcga cccgttcgtg accgacgagc ctctcgacat ggagagcaac
     7801 gtgctcaaca tggtcgacgg gtcgagcgcg tcggcggagg ccctgcggca ggcgctcgcc
     7861 gctgcgggcg ccgatgcctc gagtcaggtg cagctcgtgg ggtactcgca gggtggagcg
     7921 gccgatgcgc gcgtggccga gtcgggagac ttcgatgtgc agggcatcct gaccttcggc
     7981 agtcccaccg ggcagatcga gctgccgacc gacatcacgg cgatcctcgt cgagcacgac
     8041 gatgacctgg tgcctgcgac gggtggccgt caggacaacc agtcggcgct gctggtgaat
     8101 cgtcgcgtct acgacgactg gcaggtgccg gccgaccagc cggtgcccgc gcacctgcgc
     8161 tccaactacg ccgagactgc ccgcatgatg gatgcctcga ccggcgaact gcgctcgacc
     8221 atcgacgcgc tcgactcgct cacccagggc gcgacccggg tcacgagcac gaactatgag
     8281 ttcgagcgga taaccggatc agcgagcgtc agcggccgtt cttagacccg atgatcggac
     8341 gcagcaggat gccgatgagc cagctgatga ggcccagcac cagagcgccg agcacccccc
     8401 accagaagct gtcgacggac aggccgaacc cgaagaggct ggacagccac gcggcgagca
     8461 gcaggagcag gccgttgacg atgagcgaga tcaggccgag cgtgaggatg tagatcggga
     8521 aggcgacgat gcggatgaag ttgccgacga tcgcgttcac gattccgaag atcgcggcca
     8581 cgagcaggta ggtgagaacg atctcgagcg tgccgcccgg ggcgaagggc acgacgtgca
     8641 ctccggcgac gatgagggtg gtgagccaca gcgcgaggga gttgatcagg agcttcacaa
     8701 agagacgcat gagcccacta tgcccgcacg cgttcaccgg ccgcaatcgc cgccgccgct
     8761 gaccgcctcc ccgtcgcgca attaggctgg ggaccgtgac gaagccccga gtgaagctgc
     8821 ggcctgaggt cgagcagatg gtgccctacc gacagggacg ggcggcagcc gacgatgcgt
     8881 tcaagctctc gagcaacgag aatccgttcg agccgctcga gtcggtgctt gaggccatcg
     8941 ccgagagcag catcaaccgc taccccgagg gctcggccgc tgctctgcgc gaccgtctcg
     9001 gcgctcgcta cggcgtctcg cccgagcgca tccaggtcgg cgcgggttcg gtgtcggtgc
     9061 tctcgcagat catctcggcc gccagtgccg cgggcgacga gatcgtgtac tcgtggcgca
     9121 gcttcgaggc ctacccgctg ctcgtcgcca cagccggcgc cacgagcgtg caggttccca
     9181 acaccgccga ccaccgtcac gacctgcccg cgatgctcgc ggccatcacc gatcgcaccc
     9241 ggcttgtgat cgtgtgcagc cccaacaacc cgaccagcac cgtcgtcacg gatgacgagt
     9301 tccgggcgtt catggctgag gtgccgtcca ccgtgctcgt ggtgctcgat gaggcgtacg
     9361 ccgagttcgt gaccgatgca gaggcggttc ggggggagag cgtcatccac gactacccca
     9421 acctcgtggt gttgcgcacc ttctcgaagg cgtacggcct cgccggcctg cgcatcggct
     9481 acgcggtggg tgaggagtac gtgatggatg ccgcccgcgc cgtcgccatt ccgctgtcgg
     9541 tcaccgagcc ggctcaacgc gccgccatcg tgtcgctggc ccacgagccc gagctcctgc
     9601 agcgcgttgc ccacctcgcg actctgcgcg accgcatctg ggagggtctc gtcgagcagg
     9661 gctgggacac cccgaagccg cacggcaact tcgtctggct gccgacgggc gacgataccc
     9721 cgatcgccgc gggcattttc gaggcgcacg gcatcgtcgt tcgagccctc ggcgagggcc
     9781 ttcgggtgag cgtcggcgag tcggagtctg tggacaaact cctaagggcg tccgcggagg
     9841 ttgtgaagat gcgacgaacg gcgcccccga cggccacgtt agattagacg aatggcctac
     9901 accgcagcga ccgtccagct gctctcggag accggcgagc tggtgtcgag cgccctctcc
     9961 gaaccgtacc ttccgatcat cgagaagctc agcgacgaac agttgcgcga gttccaccgg
    10021 cagatggtcg tgttccgcgc catcgacgtc gaggcggcca accttcagcg tcagggccag
    10081 ctcgcgctct ggatccccag cctcggccag gaggccgcgc aggtcggctc cggcttcgcg
    10141 gccaaaccgc aggaccacat cttcccggcg taccgcgaac acgtggtggg gcgcatccgc
    10201 ggggtcgaca ccatcaagat catcgagatt ctgcgcggcc tcagccacgg tgggtgggac
    10261 ccggccgagc acggcaactt ccacctgtac acgctcgtca tcggatccca gaccctgcac
    10321 gccaccggct acgcgatggg catcacgctc gacgggcggg cggcaacggg cgaccccgat
    10381 aaagacgagg cggtcatcgt ctacttcggt gacggggcat ccagtcaggg ggatgtcagc
    10441 gaggctctcg tcttcgccgc gagcttccag actcccgagg tcttcttcct gcagaacaat
    10501 cactgggcga tctccgttcc ggtgacccgt cagtcgcgca caccgctcta cctgcggccg
    10561 agcggcttcg gcattcccgg cgtgcagatc gacggcaatg acgtgctcgc cagctacgcg
    10621 gtcacgaccg cgagcctcga cgcggcgcgc agcgggggcg gcccccagtt catcgaggcg
    10681 ctcacctacc gcatgggtgc gcacaccacc agcgacgacc ccaccaagta tcgggatgac
    10741 gccgaactcg acttctgggt caggcacgac ccgatcgtgc gcttcgaggc ctacctccgc
    10801 tctcgcgggg ccgacgacgc gttcttcgcg gaactcgcgg aggaggcgca cgacgcggcc
    10861 aacgacatcc ggctgcgcac cctcgccctc ggcgtgcctg atgcgtcgtc gatgttcacc
    10921 cacgtctaca ccgagcccca ctcgctcatg caggaacagc aggcctggct cgaggcctac
    10981 gaggcgtcgt tcgagggggg tgcttcgtga gcatcgaaac actgagcatg gcgaaggccc
    11041 tcaacaacgg tctgcgcaag gcgctcgccg acgaccccaa ggtgctgctc atgggcgagg
    11101 acatcggccc gctcggcggc gtgttccgtg tgaccgaggg actgcaggcg gagttcggcg
    11161 cgaaccgcgt catcgacacc ccgctggccg agtccggcat cgtcggtacg gcgatcggcc
    11221 tcgccatgcg cggctaccgc cccgtgtgcg agatccagtt cgacgggttc atcttcccgg
    11281 gcttcgacca gatcacctcg cagctcgcga agctcaccgc acggcacgag ggttcgctga
    11341 gcatgccggt cgtcatccgg gtgccgttcg gcgggcacat cggcgccgtc gagcaccacc
    11401 aggagagccc cgaggcgtac ttcgctcaca ctcccggcct gcgggtggtc agtccgtcca
    11461 cacctcacga tgcgtactgg atgatccagg aagcgatcgc atccaacgac ccgatactgt
    11521 tcttcgagcc gaagagccgg tactggccga agggcgacgt ggatacctcg gccccgggca
    11581 tcccgttgca tgccagccgg gtggtgcgcg tgggcaccga ggtcaccctc gtcggctacg
    11641 gaccgatggt gccgatgctg ctgcaggccg cggagatcgc ggccgaggag ggcacttcga
    11701 tcgaggtcgt cgacctgcgg tcgatctccc ccatcgacta cggcccgctg gtcgccagcg
    11761 tgcagaagac cggccgcctg gtcgtcgcgc acgaagctcc cgggttcgtc tcggtcggct
    11821 cggagatcgc ggcgaccgtc accgagcgtg cgttctactc gcttgaggcc cccgtcatcc
    11881 gggttgccgg attcgacacc cccttcccgc cggcgaaact cgaagccgcc tacctgcccg
    11941 accccgaccg catcctcgat gccgtcgacc gggccctggc ctactaggag agcatcatgg
    12001 cgaactcgga cttcacgctt cccgacgtcg gcgaaggtct gaccgaggcg gagatcgtct
    12061 cgtggaaggt caaggccggc gacaccgtca cgctcaatca ggtgctcgtc gagatcgaga
    12121 ccgccaagag cctggtcgag ttgccgtcgc cgttcgaggg caccgtagcg gccctgctcg
    12181 tcgaggaggg tcagaccgtc gaggtcggta ccccgatcat caccgtgtcc tcttcggggg
    12241 atgccccggc ccccgcagcg cctgccaccc cggtcgtcga ggcggtcgcc gacaccgccg
    12301 caaccgtggc ggcacccgag aagcccgccg aggagaagcc gggtgctgtg ctcgtcggct
    12361 acggcagcag cggccacggg tcgtcccggc ggcggcgcgg cgccggaccc gcggcgacgg
    12421 ccgatgcgac tgtcgcgccc gcggcgcgac ccgcgagcgt gccggcggcc gcggcatccc
    12481 cgatcatcgc gaagcccccc attcgcaagc tcgccaagga tctcggcgtc gacctcgcga
    12541 cggtgctcgc gaccggtctc gcaggcgaga tcacccgcga cgacgtgatc cgccaggcgt
    12601 cgcaggcgag cgtgttccgc aacatcgaga ctcccgagtg gggtgaccag cgcgaggagc
    12661 gcatcccggt gaagggcgtg cgcaaggcga tcgccaccgc gatggtgcag agcgccttct
    12721 cggcaccgca cgtgtcgatc ttcaccgacg tcgacgcgac ccgcaccatg gagttcgtca
    12781 agcgcctcaa gaactcgccc gacttcgctg gggtgcgagt gagcccgctg ctcatcatgg
    12841 cgaaggcgct gatctgggcc gtgcgtcgca acccgaacgt caactcgtcg tggacggaca
    12901 aggagatcat cgtccaccac tacgtgaacc tcggcatcgc ggcggccacg ccccgcggac
    12961 tcatcgtgcc caacgtcaag gacgcccact cgctctcgct catcgacctc gcgaccgcgc
    13021 tcgagcagct gaccatcacg gcccgcgacg gcaagaccca accagccgac atggccaacg
    13081 gcacgatcac gatcaccaac atcggctcgt acggcatgga caccggaacg ccgatcatca
    13141 acccgggcga ggcgacgatc gttgccctcg gcacgatcaa gcagaagccc tgggtcgtcg
    13201 atggcgaggt gcggccgcgc ttcgtcacta ccgtgggcgc aagcttcgat caccgcatcg
    13261 tcgacgggga tgtcgcgagc cgcttcgtcg ccgacctcgc gagcgtgctc gaggaacccg
    13321 ccctgctcct ggactgagca ggcgcggtct gcgggcgccc gcaccgcccg ccccgcccgc
    13381 cccgcaccga tgttcataac tcctgcacat cgggggtgac cgggtaccta cccccggtag
    13441 ctgctggcct acacctgccg cggccgccga atttgcagga gttatgaaca tcggcgtcag
    13501 gcgagcgctg cgaggcttgc cccgatactt gcgcggagac tttgccctgt ggcactctat
    13561 tgataacgaa tctcattacg gttatcagaa ggtcgcacgt gaagctcttc gcactcctcg
    13621 ccgctgcagc gctcctcctc accggctgcg cagccaccac tccggccgcg gatgacacga
    13681 tccgcatcgt cgcgtccacc gacgtctacg gcgacttcgc gcagaccatc ggaggtgaca
    13741 ccgtcacggt cacgagcctc attgccggag cgtcgcgcga tccccacagc ttcgaggctt
    13801 cggcccagga ccagctcgca gtgaacaagg ccgacgtggt gatcgagaac ggcgggggat
    13861 acgacccctt cgtcgaaacc ctgctcgcgg catccgggtc gacggcgaca gtgatctccg
    13921 tagcggaact ggtgaacctc cccgagggca cgaacgagca tctctggtac gacttcggtg
    13981 cgatggactc cttcgccaag aagctggcgg acaccctcgc tgctctcgac cccgagaacg
    14041 ccgccgagta cgcgagcaac tacgagaccc tctcggggcg actcgacggt ctcactcaga
    14101 aggtcacggg cctcgctgac ggtcagggcg tcgccgtgac cgagccggtg ccgttgtacc
    14161 tgctggaggc ggcgggtctc gtcaaccgca cccccgccga cttcaccgag gcgatcgagg
    14221 agggcagcga cgtgccgccc gcggcacttc aggagaccct cgcgctgttc ggcgacggcg
    14281 cggtcgtgct cctcgcctac aacgaccaga ccgccagtcc cgagacggaa cgggtgcggg
    14341 ccgcggccga agcggcgggc atcccggtcg tcgacttcac ggagaccctg cccgacgggc
    14401 agacttacct ctcatggatg acggcgaaca tcgacgcact cgcagcggtg ctgtgacccc
    14461 cgctctccag atcactcagg gtgcgctcgg cttcggcggg cgcaccctct ggcgtgagct
    14521 cgacctgagc gtgcgcccgg gggagtttct cgcggtgctc ggctcgaacg ggtcgggcaa
    14581 gacgagcctg ttgcgcgcca tcctcggcct gcagccgctc gacaccggca cgatccagat
    14641 cgacgggatg cccgcccgcc g
//
LOCUS       DAOSOU010000027        13889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4436_length_13889_cov_5.795432, whole genome shotgun sequence.
ACCESSION   DAOSOU010000027 DAOSOU010000000
VERSION     DAOSOU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_13889_cov_5.795432"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..889)
                     /locus_tag="PLW15_02605"
     CDS             complement(140..889)
                     /locus_tag="PLW15_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT33322.1"
                     /translation="MPQSIRSTTAARAAVFAPVAGGGRAELVAQRLTDAIELGLLSHG
                     ERLPSETEMARQFGVATVTTREALEALRELGLVETRRGRDGGSFVIAEGSRTARIDRR
                     VKALSRVELRDMGVYYSAIAAMAAELAADRSTPDDIQHLHATIDATDATAETSSRRGE
                     GTFRLEVAALSQSARLVREELRLQAEFAPLLWLCLREETHRVRSREAHLAVVAAIEDF
                     DGETARRLTVAHIAGAIEWLLDAKAELERRG"
     gene            973..2010
                     /locus_tag="PLW15_02610"
     CDS             973..2010
                     /locus_tag="PLW15_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33323.1"
                     /translation="MTSATPAIRLTGLTKTFGSVNAVDQLDLEIGEGEFFSMLGPSGS
                     GKTTVLRLIAGFELPTSGTVELQGVDVTHRAPFDRDVNTVFQDYALFPHMTVLENVAY
                     GLRVRGVGRAERAERAEAALASVALAGFGGRKPSQLSGGQRQRVALARATVVQPKVLL
                     LDEPLGALDLKLREQMQVELKELQRSLGITFIFVTHDQEEALTLSDRIAVFNHGHIEQ
                     LGTPTEIYESPSSPFVASFVGTSNIFDPSEAERMLGRASTVSIRPEKIALHTPASKPA
                     AGTVSLPGTISEVIYLGTSTRILVELEGGKRLTVLEQNASSRSTLDRRGEYVHARFAS
                     ADLVTLEPVAA"
     gene            2054..3226
                     /locus_tag="PLW15_02615"
     CDS             2054..3226
                     /locus_tag="PLW15_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT33324.1"
                     /translation="MRITATHRRTMLAGLALASSVALLSACGTSSGSEGSGEEATSLG
                     EMEGSVSILAWPGYVEDGSNDPTVDWVTPFEEQTGCQVTSKSYGTSDEAFSLMKTGDY
                     DVVAASGDASLRLVAAGDVAPVNTDLLKNYDGIFDFLKMQSWNSVDGVSYGIPHGYGA
                     NLLMYNTDVFPDGLTSWSAVFDDASANAGKVTAYDSPIYIADAAVYLMATQPDLGITN
                     PYALDETQLAAAVDLLKAQRANIGEYWSDYLKAIQAFETGDSVVGTTWQVIQNYVTTG
                     NTAVVLPDEGTTGWSDTWMISSTAKSPNCAYAWLDYIASPEANAQATAYFGEAPSSLA
                     ACDFRDDCEAFHAGDADYASKIWYWSTPIAKCLDGRTDVQCTDYSQWTTAWSEIKG"
     gene            3227..4141
                     /locus_tag="PLW15_02620"
     CDS             3227..4141
                     /locus_tag="PLW15_02620"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33325.1"
                     /translation="MTSTPTTARAGDSLKESPARKVSVFLSQHARVRLGLLLSAPLFW
                     LGLVYIAALAALLITAFWTVDSFTGQVQTTWTLDNIITVLTGSLYQTVTLRTVGVALL
                     VTIVDILVALPIAFFMAKVASPRLQRILVIAVLMPLWASYLVKVYAWRSVLSQGGIAE
                     WIAQPFGGSSPGYGLPATIITLAYLWLPYVILPIYAGLERVPNSLLEASSDLGAKTWR
                     TLGSVVLPMLWPAIIAGSIFSFSLSLGDYITVNIVGGANQLLGNLVYTNVGAANNLPL
                     AAAIALIPIAIMIVYLTAARRTGALNNL"
     gene            4171..4971
                     /locus_tag="PLW15_02625"
     CDS             4171..4971
                     /locus_tag="PLW15_02625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33326.1"
                     /translation="MRLSRAARVTLGTVTTLILAVIYLPLVVVFVNSFSTSTNLTWPP
                     PGFTLEWWGKAFQNAGALEGITTSVQIALAATAISLILGTMISLALQRFEFFGRDAVS
                     LLVILPIALPGIVTGIALNNFFRTILDVRLGIATIIVAHATFCIVTVFNNVIARLRRM
                     GTSLEEASADLGAGVWTTFRLVTFPQLRSALLAGGLLAFALSFDEIIVTTFTAGAGVT
                     TLPIWILDNLFRPNQAPIVNVIAVVLVVVSIVPIYLAQRLSGSEQLQK"
     assembly_gap    5174..5273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5274..5545)
                     /locus_tag="PLW15_02630"
     CDS             complement(<5274..5545)
                     /locus_tag="PLW15_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2781 domain-containing protein"
                     /protein_id="HOT33327.1"
                     /translation="MHPENLPLRQRPIDIVFAVFFTLFIVTSCIADMLPTVGIDFSQP
                     NGTFLVDSNYWYAHDADPLFMNPPVWMRIVTGLSAFVYLAFYLVLVP"
     regulatory      5566..5676
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5702..6310
                     /locus_tag="PLW15_02635"
     CDS             5702..6310
                     /locus_tag="PLW15_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT33328.1"
                     /translation="MSASTTIPTSRFAWRVVDIVVASVVGVASGLLFVVWNIASVPVT
                     DPLKALLPGLQALGGGFWLFAGPLTALIVRKPGAALFGELIAAVVSALVGNQWGALTL
                     LSGAVQGLGAELVFAAFLYTNWRLGVALLAGAGAGLAMAITDLTVWYPGSDTLFVTVY
                     TISGVVGGALIAGLLSWLAVRGLARTGALSRFAAGREIATRV"
     gene            6376..7728
                     /locus_tag="PLW15_02640"
     CDS             6376..7728
                     /locus_tag="PLW15_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33329.1"
                     /translation="MRGLDLSIAPGERVLLLGESGAGKSTLLRALAGVLAPDEGDAEG
                     SLLIDGAAPAPGRAGLVLQDPDSQVILARVGDDVAFGCENLGVARDEIWQRVRHSLDE
                     VGLAVPLDRATANLSGGQKQRLALAGVLAMRPGLLLLDEPTANLDPQGVTEVRDAVGR
                     VLDATGATLVVVEHRVAVWQDLVNRVIVLGTDGLIADGSPTEVLDRRGADLAARGIWV
                     PGHPPARPARRPRPAAQVELTARALSVGRTDAVLEGVRLDVSSAQGLAITGPNGSGKS
                     TLGLTLAGLLPPVAGDLTASDDLRRGIPPRPSKWRSRDLLTRIGTVFQEPEHQFLAPT
                     VRAELAIGPRALKLPQAEIDARVGELLDRLRLTHLAEANPFTLSGGEKRRLSVATVLA
                     TRPRLLVLDEPTFGQDSRTWSELVAILAELIDDGSGLVMMTHDSDLVATLADATLELS
                     "
     gene            7725..8468
                     /locus_tag="PLW15_02645"
     CDS             7725..8468
                     /locus_tag="PLW15_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOT33330.1"
                     /translation="MSVNPVAKLGAALLLSLCLILTIDWVSAAVALGLELVLFLVLRP
                     RISGFWRRTLAVWIAAPLTAVTIALYGQPSGTVYAEFLFARISEGSVTLAIATFLRVL
                     AIGLPAVVLFVTIDPTELADGLAQRVHLPVRFVLGALAGMRLVGLFLDDWKYLALARR
                     ARGGADRGRVRRLGGQAFALLVLSIRRGSALATAMEARGFGSATARSWARDSPWRARE
                     WVALATGLAVGLTAIGAAIAAGTWNFIVG"
     gene            8458..8961
                     /locus_tag="PLW15_02650"
     CDS             8458..8961
                     /locus_tag="PLW15_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT33331.1"
                     /translation="MSARRRILIDGRSGSGKTELARSIAAHWPEAQLVRLDDLYPGWD
                     GLDAGSAAVPGILTDFRWQAWDWAADRPGDWHDLDPARPIIVEGVGAISAASRPLADL
                     ALWVQLDDATRKQRALARDGDTYTPHWDRWAAQELRYLARENPQARADVTIALDADAR
                     PGAIVEP"
     gene            8958..9842
                     /locus_tag="PLW15_02655"
     CDS             8958..9842
                     /locus_tag="PLW15_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT33332.1"
                     /translation="MTSPFISVSELADELAGPTPPAVLDASLILHTATFDGDYRRESG
                     RPRWVAGHIAGSRHVDVASQFSDTTSPLHYTHPEPQAIADELARLGIRKAQHVVVYDS
                     TGTLWAARLWYLLTWIGVPTRVLDGGYAAWVAGEQPVETGETAPAEPVPSWPADAVRR
                     AWVSKQELLERAADDDRPLVCGLPAGSFTGTDPSRYARRGHIPGSVNVSSRDLFTSDG
                     HVRSRVELILAYDTAGVDVAATATPEVLLYCGGGISAAANALTLAAIGTTAVRIYDGS
                     LEEWTADPSLPLELGEVR"
     gene            complement(9839..10450)
                     /locus_tag="PLW15_02660"
     CDS             complement(9839..10450)
                     /locus_tag="PLW15_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33333.1"
                     /translation="MSAIRGTRPRSVRWASTLILTAALVPGLSACSLLTSAEGETPLT
                     GIAQCSLGHTWTTDLTDVAEQVKAALTADQISVTDVTATGTQTLEWSIEGHVVLTPDY
                     TLTITTVPATDQILTIKQTHSGKATGAAYISGEVAIPRKWDGTGVTIDTVADNNGTVV
                     EDLPFAVPQTSFDDAVGLEITCSGDTLTIHPRGSDVTQTWKRS"
     gene            complement(10507..11145)
                     /locus_tag="PLW15_02665"
     CDS             complement(10507..11145)
                     /locus_tag="PLW15_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33334.1"
                     /translation="MFHAHRAVPFAAAAALLLTLAGCSLGGGEEPGSTGTDPGSSTGT
                     DSGTDSGAGAASCVEDRTWVLDTDDLAAQLATQMSSGGMTVTESTAEGRQSIEFASDG
                     TASASVDVTYTLSIDSGDGIVITVVQTHSGEPRGQWEWVGDSTTLTFSGWDNAGYSVQ
                     NTFLINGVASDSPISIPSDTLGDTDMETTCSGDALSTHVATSPFTQHWIAEG"
     gene            complement(11221..11577)
                     /locus_tag="PLW15_02670"
     CDS             complement(11221..11577)
                     /locus_tag="PLW15_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOT33335.1"
                     /translation="MKVDELDEDAFERLVVDELDLLPDDMVDGLDNVVFVTESRPEDG
                     SLDLLGLYDGVAMTERGAYGFGELPDRIVLYREPLLAISADLEELKDQIHVTLVHEIA
                     HFYGLDDAQLHELGWA"
     gene            complement(11574..12080)
                     /locus_tag="PLW15_02675"
     CDS             complement(11574..12080)
                     /locus_tag="PLW15_02675"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HOT33336.1"
                     /translation="MPFRPGDEGVTVRFTRNEALVLADLAGELIDLLDQRGPDATGDM
                     LYAQLGIGGSSTPPLDPALARLLPDAYRDDEDAASDHRRLTERSLVDRKLANARRVLA
                     DLDDPVVVLDEQGIQSWLRTLTDLRLALAARLQIEEDGDTGLGDEDMLMIYDWLGYLQ
                     GSLVEVIE"
     gene            complement(12080..12370)
                     /gene="clpS"
                     /locus_tag="PLW15_02680"
     CDS             complement(12080..12370)
                     /gene="clpS"
                     /locus_tag="PLW15_02680"
                     /inference="COORDINATES: protein motif:HMM:NF000668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOT33337.1"
                     /translation="MSQTLESPRVELDEASAPDPPWVTIVWNDPVNLMTYVSWVFRSY
                     FGHSREEAERLMLLVHTEGRAVVASGGREEMERHVEAMHDFGLWATLTREDS"
     gene            complement(12426..12501)
                     /locus_tag="PLW15_02685"
     tRNA            complement(12426..12501)
                     /locus_tag="PLW15_02685"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12466..12468),aa:His,seq:gtg)
     gene            complement(12602..13222)
                     /gene="orn"
                     /locus_tag="PLW15_02690"
     CDS             complement(12602..13222)
                     /gene="orn"
                     /locus_tag="PLW15_02690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOT33338.1"
                     /translation="MASSSDLLVWIDCEMTGLDVSIDELVEVAVVITDYDLVPVDPGF
                     SVVIKPDQAAWDNMAEFVLNMHRESGLIEEIPHGVSLADAEFAVNEYILKFVPTPKTA
                     PLAGNTIGTDRTFLAKYMPRVDSHLHYRSIDVSSIKELSRRWFPRVYFNAPTKDGGHR
                     ALADILESIRELRYYREAVFVAEPGPTTEQVQTIAARVVEAFAQPQ"
     gene            13300..13572
                     /locus_tag="PLW15_02695"
     CDS             13300..13572
                     /locus_tag="PLW15_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOT33339.1"
                     /translation="MTGTPELSRGDEYIVFYTGGPYDGQTDRRISTDGTWESEITVLA
                     AVDGKETQLVYASPVAHAVGEQVQVTYSWDQPDSEPLEALDERNDD"
     gene            13607..13867
                     /locus_tag="PLW15_02700"
     CDS             13607..13867
                     /locus_tag="PLW15_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT33340.1"
                     /translation="MNLLFFSSSFCDPCIATRQVLAEAWALVPAARIEELDVALAPDE
                     AERAGIRVTPTVVVLRDDGTEVFRAEGVPSVNQVLVALAKAV"
BASE COUNT         2162 a         4736 c         4566 g         2325 t
ORIGIN      
        1 cgtgacagcg ccgcggtgtg caccgacgtg ccgttcgatc gctgcgcgcc acgagtcgat
       61 ctgctcgaaa accgttccga aggtggtgtc gacggcggtg acggcatcga cgactgagga
      121 ttgtgtcgct gttgtcatgt cagcccctcc gttcgagctc ggccttggcg tcgagcagcc
      181 actctatggc tccggcgatg tgtgcgacag tcaggcgcct ggccgtctcg ccgtcgaagt
      241 cctcgatcgc ggccaccacg gcgaggtgcg cctcgcggct gcgcacgcgg tgggtctcct
      301 cgcgcaggca gagccagagc agcggggcga actcggcctg caggcgcaac tcctcccgca
      361 ccagccgggc ggactgcgag agcgcggcga cctccagccg gaaggtgccc tcgcccctgc
      421 gtgatgaggt ttcggccgtg gcatccgtcg cgtcgatggt ggcgtgcagg tgctggatgt
      481 cgtcgggcgt cgagcgatcg gccgcgagct cggcagccat ggccgcgatc gcggagtagt
      541 acacgcccat gtcgcgcagt tcgacccggg agagcgcctt cacgcgacgg tcgatgcggg
      601 cagtacgtga gccttcggcg atcacgaagc tcccgccgtc gcgcccgcgc ctggtctcga
      661 cgagcccgag ttcgcgcagc gcctcgagcg cctcgcgcgt ggtcacagtg gcgacgccga
      721 actggcgggc catctccgtc tcgctcggca gacgctcgcc gtgggagaga aggcccagtt
      781 cgatggcatc ggtgagccgc tgcgcgacga gctcggcacg gcctccgccg gcgaccggcg
      841 cgaagaccgc cgcccgcgct gctgtggtcg aacggatgga ctggggcatc gtgacacgac
      901 cgtaacatgc ccgagacttg catgacatct gcttccatat gatttactac aggacaggca
      961 aggaggccct gcgtgacatc agctacgcct gcaattcggc tcaccggact gaccaagacg
     1021 ttcggctcgg tcaacgcggt cgaccaactc gatctggaga tcggcgaggg agagttcttc
     1081 tccatgctcg gtccgagcgg gtcaggcaag accactgtgt tgcgactgat cgccgggttc
     1141 gagttgccga cctccggaac cgtcgagctg cagggcgtgg atgtcacgca ccgcgcgccc
     1201 ttcgaccgcg acgtgaacac cgtcttccag gactacgcgc tcttcccgca catgaccgtg
     1261 ctggagaacg tcgcgtacgg gctgcgggtg cggggggtgg gtcgcgcaga acgcgcggag
     1321 cgtgccgagg cggccctggc atccgtcgcc ctcgcgggct tcggcggccg caagcccagc
     1381 cagctctccg gtggccagcg ccagcgtgtt gctctcgccc gagccaccgt cgtgcagccc
     1441 aaggtgctgc tgctcgacga gccgctcggc gccctcgacc tcaagctgcg cgagcagatg
     1501 caggtcgagc tcaaggagct gcagcgttcg ctcggcatca cattcatctt cgtgacgcac
     1561 gaccaggagg aggcgctgac gctcagcgac cgaatcgcgg tcttcaacca cggacacatc
     1621 gagcagctcg gtacgcccac cgagatctac gagtcgccgt cgtcgccgtt cgtggcctcg
     1681 ttcgtgggca cgtccaacat cttcgacccg agcgaggccg agcggatgct gggccgggca
     1741 tccacggtct ccatccgccc cgagaagatc gccctccaca cgcccgcgtc gaagccagcc
     1801 gccggaaccg tgtcgctgcc cggcaccatc agcgaggtga tctacctcgg cacgtccacg
     1861 cgcatcctcg tggagctcga gggcggcaag cgcctcacgg ttctcgagca gaacgcgagc
     1921 tcgcggtcga ccctcgaccg ccggggagag tacgtgcacg ccagatttgc cagcgccgat
     1981 ctcgtcaccc tagagcccgt cgcggcctga tagatcccgt accccagtcc ccaccagaga
     2041 aggagcacca gcaatgcgaa taacagcaac ccaccgccgc acgatgctcg cgggcctcgc
     2101 gctcgcgtca tccgtcgcac tcctgagcgc ctgcggaacc agttccggtt ccgaaggctc
     2161 aggcgaggag gccaccagcc tcggagagat ggagggcagc gtctcgattc tcgcgtggcc
     2221 cggctacgtc gaagacggca gcaacgaccc gaccgtcgac tgggtcaccc ccttcgagga
     2281 gcagaccggc tgtcaggtca ccagcaagag ctacggaacc tccgacgagg cgttcagcct
     2341 catgaagacc ggcgactacg acgtggtggc cgcctccggt gacgcgagcc tgcgactcgt
     2401 cgcggccggc gatgtcgccc cggtcaacac cgacctgctc aagaactacg acggtatctt
     2461 cgacttcctc aagatgcagt cgtggaacag cgtcgacggc gtgagctacg gcatcccgca
     2521 cggctacggc gccaacctgc tcatgtacaa cacggacgtc ttccccgacg gcctgacctc
     2581 gtggagcgcc gtgttcgatg acgcctccgc gaacgccggc aaggtcaccg cctacgactc
     2641 gccgatctac atcgcggatg ccgcggtcta cctcatggcg acgcagcccg acctcggcat
     2701 caccaacccc tacgcgctcg acgagacgca gctggcggcc gccgtcgacc tgctgaaggc
     2761 ccagcgcgcc aacatcggcg agtactggag cgactacctc aaggcgatcc aggcgttcga
     2821 gaccggtgac agcgtcgtcg gtaccacctg gcaggtcatc cagaactatg tcactacggg
     2881 taacaccgcg gtcgtgctgc cggatgaggg aaccactgga tggtctgaca cctggatgat
     2941 ctcgtcgacg gcgaagagcc cgaactgcgc ctacgcgtgg ctcgactaca tcgccagccc
     3001 tgaggccaac gcgcaggcga cggcgtactt cggcgaggct ccgtcgagcc tggccgcgtg
     3061 cgacttccgt gacgactgcg aggccttcca cgccggtgac gccgactacg cgtcgaagat
     3121 ctggtactgg tcgaccccga tcgcgaagtg cctcgacggt cgcaccgatg ttcagtgcac
     3181 cgactactcg cagtggacga ccgcctggtc ggagatcaag ggctaagtga cctccacccc
     3241 cacgaccgcg cgtgccgggg actccctgaa ggagtctccg gcacgcaagg tctccgtatt
     3301 cctgagccag cacgcccggg ttcgcctcgg gctgctgctc tcggcacccc tcttctggct
     3361 cggcctcgtc tacatcgccg cgctggccgc gctgctgatc accgccttct ggaccgtcga
     3421 cagcttcacc ggccaggtgc agaccacctg gacgctcgac aacatcatca cggtgctgac
     3481 cggatcgctg taccagacgg tcacgctgcg cacagtgggc gtggcgctgc tggtgacgat
     3541 cgtcgacatt ctggtcgcgc tacccatcgc cttcttcatg gcgaaggtcg cgagcccgcg
     3601 gctgcagcgc atcctcgtca tcgcggtgct catgccgctg tgggcgagct atctcgtcaa
     3661 ggtctacgcg tggcgctccg tgctctcgca gggcgggatc gccgaatgga tcgcgcaacc
     3721 cttcggtggc tccagccccg gctacgggct acccgccacg atcatcaccc tcgcctacct
     3781 gtggcttccc tacgtgatcc tgcccatcta cgccggcctc gagcgggtgc cgaactcgct
     3841 gctcgaggca tccagtgatc tgggggcgaa gacgtggcgc acgctcggct ccgtcgtgct
     3901 gccgatgctg tggccggcga tcatcgcggg ctcgatcttc agcttctcgc tctcgctcgg
     3961 cgactacatc accgtcaaca tcgtcggtgg ggcgaaccag ctgctcggca acctcgtcta
     4021 cacgaacgtg ggtgccgcca acaacctgcc gctcgcggcc gcgatcgcgc tgattccgat
     4081 cgccatcatg atcgtctacc tcacggccgc ccgccgcacg ggcgcgctca acaacctcta
     4141 ggagccccgg atgaccgtca ggagcacccc gtgagactga gccgcgccgc gcgcgtcacc
     4201 ctcggcaccg tgaccacgct gatcctcgcc gtgatctacc tgccgctggt cgtggtgttc
     4261 gtgaactcgt tcagtacgag caccaacctc acctggccgc cgcccggctt cacgctcgag
     4321 tggtggggca aggcgttcca gaacgcgggc gccctcgagg gcatcaccac gagcgtgcag
     4381 atcgcgctgg ccgccaccgc gatctcgctg atcctcggca cgatgatctc gctcgccctg
     4441 cagcgcttcg aattcttcgg tcgggatgcc gtcagcctgt tggtcatcct gccgatcgcg
     4501 ctgccgggca tcgtcaccgg tatcgcgctc aacaacttct tccgcaccat cctcgacgtg
     4561 cggttgggga tcgcgaccat catcgtcgcg cacgcgacct tctgcatcgt gacggtgttc
     4621 aacaacgtca tcgcgcggtt gcggcggatg ggcacctcgc tggaggaggc atccgcggat
     4681 ctgggtgccg gggtgtggac cacgttccgg ctggtgacgt tcccgcagct gcgctctgcg
     4741 ctgctggccg gtggcctgct cgcgttcgcg ctctcgttcg acgagatcat cgtcaccacg
     4801 ttcactgcgg gggcgggcgt gacgaccctg ccgatctgga tcctcgacaa cctgttccgc
     4861 cccaaccagg cgccgatcgt caacgtgatc gccgtcgtgc tcgtggtcgt gtcgatcgtg
     4921 ccgatctacc tcgcgcagcg gctgtcgggg tcggagcagc tgcagaagta ggggtgcggg
     4981 tcttgagtgc gcacacaccc cgccccagta cttcgtcggg atcgcctaga tgatgggtgt
     5041 tgagcggtcc ttacccggta tctaggcgaa ctcggcgatc ctgccgccgc cacggagtcg
     5101 tcggtgacgg cggatgcgcc tgacgctaga acttccgcgt gaacggcagc ggcttgcgca
     5161 tgcgcacgag cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcacca
     5281 gcaccaggta gaacgccagg tagacgaagg ccgacaagcc ggtgacgatg cgcatccaga
     5341 ccggcgggtt catgaacagc gggtcggcgt cgtgtgcgta ccagtagttc gagtcgacga
     5401 ggaaggtgcc gttcggctgg gagaagtcga tgccgaccgt cggcagcatg tcggcgatgc
     5461 acgaggtgac gatgaacaac gtgaagaaga ccgcgaacac gatgtcgatg ggccgctgac
     5521 gtaggggcag gttctccgga tgcacggcct cacgctacta gaatggaagc tgtacacggg
     5581 agtccggtga gccgggctga gaggaagctt ctccaagctt cgaccgtcga acctgatctg
     5641 gatcatgcca gcgcagggag gcctctttga atcccgtgcc attcatcagg aagggcacgt
     5701 catgtctgca tcaacaacca tccccacctc acggttcgcc tggcgagtcg tcgacatcgt
     5761 cgtggcgagc gtcgtcggcg tcgccagcgg tctgctgttc gtggtctgga acatcgcgtc
     5821 ggtaccggtg accgatccgc tcaaggcgct cctgccgggc ctccaggccc tcggcggcgg
     5881 gttctggctc ttcgccgggc cgctcactgc actcatagtg cgcaagcccg gtgccgccct
     5941 cttcggcgag ctcatcgccg cggtcgtctc ggccctcgtc ggcaaccagt ggggtgcgct
     6001 cacgctgctg agcggtgccg tgcagggtct cggcgccgag ctcgtgtttg cggcgttcct
     6061 gtacacgaac tggcgtctcg gtgtcgccct gctcgccggc gctggtgctg gtctcgcgat
     6121 ggcgatcaca gacctcaccg tctggtatcc cggttcggac accctgttcg tcacggtcta
     6181 cacgatctcg ggcgtcgtcg gcggcgcgct catcgccggg ctgctgtcgt ggctcgcggt
     6241 gcggggactg gcgcgcaccg gggctctcag ccgtttcgcc gccggtcgag agatcgcgac
     6301 gcgggtctga ccgagccggc gcgcgtctcc gcctccggct gggggtggcg accggccgcg
     6361 cgcctggcgt gggcgctgcg cggcctcgac ctgagcatcg cacccggaga gcgggtgctg
     6421 ctgctcgggg agtccggcgc gggcaaatcc acgctccttc gcgccctcgc cggcgtcctc
     6481 gcgcccgatg agggtgatgc ggaaggatcc ctgctcatcg acggcgcggc cccggctccc
     6541 gggcgggccg ggctcgtgct gcaggatccg gactcccagg tcatcctcgc ccgggtgggc
     6601 gacgacgtcg ccttcggctg cgagaacctc ggtgtcgccc gcgacgagat ctggcagcgc
     6661 gtgcgccaca gtctcgacga ggtcggtctt gcggtgccgc tcgaccgcgc caccgcgaac
     6721 ctctcgggcg gccagaagca gcgcctcgcc ctggcgggcg tgctcgccat gcgacccgga
     6781 ctgctcctgc tcgatgagcc cacggccaac ctcgacccgc agggcgtgac cgaggttcgg
     6841 gatgccgtcg gccgggtcct cgacgccacc ggcgcgaccc tcgtggtcgt cgagcatcgc
     6901 gtcgccgtgt ggcaggacct cgtcaaccgc gtgatcgtgc tgggcaccga cggactgatc
     6961 gccgacggca gcccaacgga ggtgctcgac cgtcggggcg ccgacctcgc cgcccgcggc
     7021 atctgggtgc ccgggcatcc gccggcccgc ccggccaggc gcccacgacc cgccgcccag
     7081 gttgagctga ctgcgcgcgc actgagcgtg ggtcgtaccg acgccgtgct cgagggcgtg
     7141 cggctcgacg tgtccagcgc acagggtctt gccatcaccg ggccgaacgg cagtggcaaa
     7201 tccaccctcg ggttgaccct ggccgggttg ctgccgccgg tcgctggtga cctgaccgcc
     7261 tcggatgacc tgcgccgggg catcccgccg cgcccgtcga agtggcgctc gcgcgacctg
     7321 ctcacccgca tcggcacggt attccaggag ccggagcacc agtttctcgc cccgacagtg
     7381 agggccgagc ttgctatcgg tccgcgcgcc ctgaagctgc cccaggccga gattgatgcc
     7441 agagtcggcg agctgctcga tcggttgcgc ctgacgcacc tcgccgaggc gaacccgttc
     7501 accctgtcgg gaggcgagaa gcgtcggctg agcgtcgcca ccgtgctcgc cacgcgcccg
     7561 cgcctgctcg tgctcgacga accgacgttc ggccaggact cccgcacgtg gtccgagctc
     7621 gtggcgatcc tcgccgaact catcgatgac gggtctggcc tcgtgatgat gacccatgac
     7681 agcgacctgg tggcgacact cgccgacgcg actctggagc tgtcatgagc gtcaacccgg
     7741 tcgcgaaact cggcgcggcg ctgctgctga gcctctgcct catcctcacc atcgactggg
     7801 tgtcggctgc cgtcgcgctc ggtctcgaac tcgtgctctt ccttgtgctt cgaccgcgga
     7861 tcagcggctt ctggagacga accctcgccg tctggatcgc tgcaccgctg accgccgtga
     7921 cgatcgcgct ctacggtcag ccgagtggca ccgtctacgc cgagttcctg ttcgcccgca
     7981 tcagcgaagg ttcggtgacg ctcgcgatcg cgacatttct gagagtgctc gcgatcgggc
     8041 tgcccgctgt cgtgctgttc gtgacgatcg accccaccga gctcgcagac ggcttggccc
     8101 agcgtgtgca tcttcccgtg cgcttcgtgc tgggcgccct ggcggggatg cgcctggtgg
     8161 gcctcttcct cgacgactgg aagtaccttg cactggcgcg ccgcgccaga ggaggtgccg
     8221 atcgcggcag agtgcgcagg ctgggcgggc aggcgttcgc cctgctcgtg ctctcgatcc
     8281 gtcgggggag tgccctcgcc accgcgatgg aggcacgcgg gttcggatcc gcgaccgcgc
     8341 gctcgtgggc acgggattcg ccctggaggg cacgggagtg ggtcgcgctg gcaaccgggc
     8401 tggccgtcgg gctcacagcg atcggggcgg ccatcgccgc aggaacctgg aacttcattg
     8461 tcggctagac gcagaatcct catcgacgga cggtcgggct cgggcaagac cgagctcgcc
     8521 aggtccatcg ccgcccactg gcccgaggcg cagctcgtgc gcctcgacga cctgtacccg
     8581 gggtgggatg gcctggatgc gggcagtgcg gccgtgccgg gcatcctgac cgacttccgc
     8641 tggcaggcct gggactgggc ggccgaccga cccggcgatt ggcacgacct cgaccccgct
     8701 cgcccgatca tcgtcgaggg ggtgggcgcg atcagcgccg ccagtcggcc cctcgccgac
     8761 ctggccctgt gggtgcagct ggatgacgcg acccgcaagc agcgcgcctt ggcccgtgac
     8821 ggcgacacct acaccccgca ctgggaccgc tgggcggccc aggaactgcg ctacctcgcc
     8881 cgggagaacc cccaggctcg cgccgatgtc accatcgcac tggatgccga cgcccgccca
     8941 ggtgcaatag tggagccatg acctcgccgt tcatctccgt gtccgaactc gccgacgaac
     9001 tcgccggccc caccccgcca gccgtgctgg atgcgagcct catcctgcac acggcgacct
     9061 tcgacggcga ctaccgacgc gagtcaggcc gtccgcgctg ggtggcgggg cacatcgcgg
     9121 ggtcgcggca cgtcgatgtc gccagccagt tcagcgacac cacctcgccg ctgcactaca
     9181 cgcatcccga gccgcaggcg atcgccgatg aactcgctcg cctcggaatc cgcaaggcgc
     9241 agcacgtcgt cgtctacgac tcgaccggca cgctctgggc ggcacgcctc tggtacttgc
     9301 tgacctggat cggcgtgccg acccgggtgc tcgacggcgg ctacgcggca tgggtggccg
     9361 gggagcagcc cgtcgagacc ggggagacgg cccctgccga gccggtgccc tcctggcccg
     9421 ccgacgccgt gcgccgcgcc tgggtgagta agcaggagtt gctggagcgc gcggccgatg
     9481 acgaccgccc gctggtgtgc ggcctccccg ccggatcgtt cacgggcacc gacccctccc
     9541 gctacgcccg gcgcgggcac atccccggca gcgtcaacgt ctccagtcgc gacctcttca
     9601 cctcagacgg ccacgtcaga agtcgcgtcg agttgatcct cgcctacgac accgccggcg
     9661 tggatgtcgc agccacagcc accccggaag tgctgctgta ttgcgggggc ggcatctctg
     9721 ccgcggccaa cgcgctcacg ctggccgcga tcgggaccac cgccgtgcgc atctacgacg
     9781 gctcacttga ggagtggacg gccgacccgt cacttccgct ggagttgggc gaggtgcgct
     9841 agctgcgctt ccaggtctgg gtgacgtcgg agccccgcgg gtgaatcgtc agcgtgtcac
     9901 cgctgcaggt gatttcgaga cccacggcgt cgtcgaagct ggtctggggc acggcgaacg
     9961 gaagatcctc gacgacggtg ccgttgttgt cggcgacggt gtcgatggtc acgccggtgc
    10021 catcccactt gcgggggatg gcgacttcgc cgctgatgta ggcggcgccg gtggccttgc
    10081 ctgagtgcgt ctgcttgatg gtgaggatct ggtcagtcgc gggcaccgtc gtgatggtga
    10141 gcgtgtagtc cggagtgagc acgacgtgcc cctcgatgct ccactcgaga gtctgggttc
    10201 ccgtcgcggt gacgtcagta actgagatct ggtcggcggt gagcgcggcc ttgacctgct
    10261 ccgcgacatc cgtcagatcg gtcgtccagg tgtgcccgag agagcactgg gcgatgcccg
    10321 tgagcggcgt ctcgccctca gcggaggtga gcagagagca cgccgacaac ccgggcacca
    10381 acgcagccgt cagaatcaga gtcgacgccc agcgcacgct cctcggtcga gttcccctga
    10441 tcgcactcat gagcaaatcc tactttctcg tggggtgcct catagacagt ggcgcctctt
    10501 tggtgcctaa ccctcagcaa tccagtgctg ggtgaatgga ctggtcgcca cgtgagtgct
    10561 cagtgcgtcg cccgagcagg tcgtctccat gtccgtgtcc cccaacgtgt cgctggggat
    10621 cgagatgggg gaatctgacg caacgccatt gatgagaaac gtgttctgca cggagtaacc
    10681 ggcgttgtcc cagccgctga acgtgagggt ggtcgagtcg cccacccact cccactgccc
    10741 gcgtggctcg cccgagtggg tctgcacgac ggtgatgacg atgccgtcgc cgctatcgat
    10801 ggacagggta taggtgacat ccacactcgc ggaggccgtg ccatccgagg cgaactcgat
    10861 gctctggcgg ccctcggcgg tcgactcagt caccgtcatt ccgccggaac tcatctgggt
    10921 ggcgagctgg gcggcgagat catcggtgtc gagcacccag gtgcgatcct cgacgcagct
    10981 ggcggcaccg gcgccggagt ccgtgccgga gtcggtgccg gtcgagctgc ccggatccgt
    11041 gccggtcgac cccggctcct cgccgccgcc aagcgagcac ccggccaaag tcaggagtag
    11101 cgctgcggct gcagcgaagg gaacggcgcg gtgggcgtgg aacatcgggg cctcgattcg
    11161 gtcgtgacgg aattctacgt ttcgctggca cgacgcatca ggaacgttat cgggttgaca
    11221 tcaagcccag ccgagttcgt gcagctgggc atcgtccaat ccgtagaagt gcgcgatctc
    11281 gtgcacgagg gtcacgtgga tctgatcctt gagctcctcc aagtcggcgc tgatggccag
    11341 aagcggttcg cggtagagca cgatccggtc cggaagttca ccgaaaccgt atgcgccgcg
    11401 ctcggtcatc gccacaccgt cgtagagccc cagaaggtcc agactgccgt cctccgggcg
    11461 ggactccgtc acgaagacca cgttgtcgag accgtccacc atgtcatcgg gcagcaggtc
    11521 cagctcatcc acgacgagac gttcgaacgc atcctcgtct agctcgtcaa ccttcactcg
    11581 atcacctcca ccagggagcc ctgcaagtat cccagccagt cgtagatcat cagcatgtcc
    11641 tcgtcgccaa ggccggtgtc gccgtcctcc tcgatctgca gcctcgcggc cagggccaga
    11701 cgcaggtcgg tgagggtgcg cagccacgac tggatgccct gctcatcgag cacgaccacc
    11761 gggtcatcga ggtcagcgag cacacgcctc gcgttggcga gtttgcggtc caccagggat
    11821 cgctcggtga gccgccggtg atcggatgcc gcatcttcgt cgtcgcggta cgcgtcgggc
    11881 agcaggcggg ccagcgcggg gtcgagaggg ggtgtggagg agccgccgat gcccagctgc
    11941 gcgtagagca tgtcgcccgt ggcatccggg ccccgttggt cgaggaggtc gatgagttcg
    12001 cccgccagat cggcgagcac gagagcctcg ttgcgggtga accgcaccgt cacgccctcg
    12061 tcaccggggc gaaatggcat cacgagtcct cgcgagtcag ggtcgcccag agcccgaagt
    12121 cgtgcatggc ctccacgtgc cgttccatct cctcgcgccc gcccgaggcg accacagccc
    12181 gcccctccgt gtgcacgagc agcatcaggc gttcggcttc ctcgcgagag tgcccgaaat
    12241 agctgcggaa cacccacgac acatacgtca tcaggttcac cggatcgttc cagacgatcg
    12301 tgacccacgg ggggtcgggt gcggatgcct cgtcgagttc gacgcgcggc gactcgagtg
    12361 tctgggacac acgaacctcc tgccgaaaag gaaaacggcc tgaccaccgg tcagaccgtt
    12421 tcacttgggg tgaacgacgg ggctcgaacc cgcgacatcc ggctccacaa gccagcgctc
    12481 tgccaactga gctacgccca ccaagaactc cgccggtaag gcgcagcctg aatagtgtat
    12541 tacatcgcgg tcggtgcggc tgcgccaacc ctcaggggag gcaccatgtt ggcgccgtgg
    12601 gtcactgagg ttgggcgaac gcctcgacga ctcgagccgc aatcgtctgc acctgctcgg
    12661 tcgtgggccc cggctcggcg acgaagaccg cctcgcggta gtaccgcagc tcacggatcg
    12721 actccaggat gtcggcaagt gctcggtgac cgccatcctt cgtgggggca ttgaagtaga
    12781 cccggggaaa ccaccggcgg gagagctcct tgatcgagga cacatcgatg gagcggtagt
    12841 gcaggtggct gtcgacccgg ggcatgtact tggcgaggaa cgtgcgatcg gtgccgatcg
    12901 tgttgccggc cagcggtgcc gtcttcgggg tgggcacgaa cttcaggatg tactcgttca
    12961 cggcgaactc ggcgtcggcc agagacacgc cgtgagggat ctcctcgatg agtccgcttt
    13021 cgcggtgcat gttgagcacg aactcggcca tgttgtccca cgccgcctgg tcgggcttga
    13081 tgaccacgct gaaacccggg tccaccggca ccaggtcgta gtcggtgatg accaccgcaa
    13141 cctcgacgag ttcgtcgatc gacacgtcca gtccggtcat ctcgcagtcg atccagacga
    13201 ggaggtcact cgatgaagcc atgggcgaaa gtctatcgtc ggggtgcgat gctcccactt
    13261 cgtgcacggc ggggccccgg agtctggtag gactgactca tgactggaac tcctgagctc
    13321 tcccgcgggg acgagtacat cgtcttctac accggcggtc cgtacgacgg ccagaccgat
    13381 cggcgcatca gcaccgacgg aacgtgggag tccgagatca cggtgttggc cgcggttgac
    13441 ggcaaggaga cgcagctcgt ctacgcgtcg cccgtcgcgc acgcggtcgg cgaacaggtg
    13501 caggtcacct acagctggga tcagcccgac agcgagccgc tcgaggctct cgacgagcgc
    13561 aacgacgact aggaatcctg atgcgcccga cgtggttggg cctcaggtga acctgctctt
    13621 cttctcctcc agcttctgcg atccctgcat cgccacgcgg caggtactcg ccgaggcttg
    13681 ggcgctcgtt ccggccgcac ggatcgaaga gttggacgtc gcgctcgccc ccgacgaggc
    13741 cgagcgggcg ggcatccggg tgacgcctac tgttgtggtg ctccgggatg acggcaccga
    13801 ggtctttcgc gccgagggag tgcccagcgt gaatcaggtc ctcgtggcgc tggctaaggc
    13861 cgtgtaggga acgctgcggt atcgtttac
//
LOCUS       DAOSOU010000028        13718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4495_length_13718_cov_5.501281, whole genome shotgun sequence.
ACCESSION   DAOSOU010000028 DAOSOU010000000
VERSION     DAOSOU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13718
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4495_length_13718_cov_5.501281"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PLW15_02705"
     CDS             complement(<1..1113)
                     /locus_tag="PLW15_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33341.1"
                     /translation="MHVLSVREAMGGPWAGNVPGWLILFVPTTVLVVLQETTIGASGW
                     AAALVLAVLEHLAAGLLVFAVVWALRRRWRVIPIGLVFAMWVGVGVVRGLVWSAWHAW
                     VLHTEADVGYRVLVWVAISLVWSPLFTYTLAQLDHRRALLGELTAVRLLRATERARVD
                     QSARERREHLIATVQSTIGPVISELHRSLTTIAPRLTRSSVTAMAKRVSSVAYDTSRL
                     VAARGPEHQAQVSHIQRAPISAALDFPPDRPLLASALAGVAMLPLVVPDTLRVQGFGF
                     ALRTILAISVMVLLLALSLAVLRFTRIRFGRPTLVALLLAFLIPGAAGSVVIALTPGW
                     PPISEMLSLLILLPVGSAIAGANVATAVGRMSSESIS"
     gene            1514..2002
                     /gene="ssb"
                     /locus_tag="PLW15_02710"
     CDS             1514..2002
                     /gene="ssb"
                     /locus_tag="PLW15_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT33342.1"
                     /translation="MTDTITLTGLVATDPRHVVTSEGLAITSFRLASTQRRFDRSQEK
                     WIDGDTNWYTITTFRQLAMNSAGSVKKGERIVVTGRLRIREWQSGERTGLNIDVEADA
                     LGHDLAWGTSTFSRSISATTASADSGSATADEPTADEPSADADETSAQSDESRPMATL
                     PF"
     gene            2074..2553
                     /locus_tag="PLW15_02715"
     CDS             2074..2553
                     /locus_tag="PLW15_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33343.1"
                     /translation="MPLRYTRHLAAIAGVVALGAVLAGCGGAPMPTPTASSSRSATPS
                     PSPTAKAPVFHPDGSAAANQQYFDYVNRALNASSGLSDGRTIVDNLVAAGFTKADMEV
                     TADTTALDLTVDSIQVAVRIKGECLIGGFSYVGYQGIVAPLLGSGTCLVGATRPIDW"
     gene            2581..>4259
                     /gene="ettA"
                     /locus_tag="PLW15_02720"
     CDS             2581..>4259
                     /gene="ettA"
                     /locus_tag="PLW15_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOT33344.1"
                     /translation="MAEFIYTMVRARKAVGDKLILDDVTMSFYPGAKIGVVGPNGAGK
                     STILKIMAGLDTPSNGEARLTPGFSVGILMQEPELDESKTVLENVQEGVAEIKDKVDR
                     HAAIGLEMAEPDADFDALLAEMGTLQEDIDAADAWDLDSQLEQAMDALRTPPGDATVA
                     NLSGGEKRRVALTKLLLQKPDLLLLDEPTNHLDAESVLWLEQHLAKYHGAVLAVTHDR
                     YFLDHVAEWIAEVDRGHLYPYEGNYSTYLQKKGERLEVQGKKDAKLAKRLASELEWVR
                     SNAKGRQVKSKARLARYEEMVNEAEKTRKLDFEELVIPVGPRLGSQVIDAKNLKKGFG
                     ERVLIDNLTFTLPRNGIVGVIGPNGVGKTTLFKTITGLEPLDSGDLKIGETVDISYVD
                     QNRGGIDPNKTLWEVVSDGQDFIQVGKTEIPSRGYVSQFGFKGPDQQKKAGVLSGGER
                     NRLNLALTLKQGGNLLLLDEPTNDLDVETLGSLENALLEFPGCAVVITHDRWFLDRIA
                     THILAYEGTEDDPANWYWFEGNFEAYEENKIERLGPDAAKPHRSAYRKLTR"
     assembly_gap    4260..4359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4368..4835
                     /locus_tag="PLW15_02725"
     CDS             4368..4835
                     /locus_tag="PLW15_02725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT33345.1"
                     /translation="MHVPIPLRWSDFDAYAHVNNAEMLRLLEEARIQAFWRPDEGSDG
                     GAVTAVLDARPGAETISLIARQEIEYLAPIPYMRAPIDIEMWIGRIGGASLEICYELF
                     SPVGVTPRVLFTRAATTLVMVTAATGKPQRIGDELRALWQPYVEPPVQFTKRG"
     gene            complement(4832..5701)
                     /locus_tag="PLW15_02730"
     CDS             complement(4832..5701)
                     /locus_tag="PLW15_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HOT33346.1"
                     /translation="MSDPLSGFLAALDLTDTGARTSEDIFTGPSLWQPHGRVFGGQVL
                     AQSLVAAQRTSPDGRVVHSMHAYFLRPGQVDLPITFSVDRIHDGRSFSTRRTQAYQEG
                     LPILSMIASFQDEDEGLEHQVDMPVGIPDPESLPSAAEHLGEIDHAVARFWATERPFD
                     MRHVPSPVYLSVEGDRVAHQAVWLKAVGRLPDDDNLHRAALAYASDYSILEPILRRHG
                     IAWAMPGLKAASLDHAMWWHRPGRVDEWLLYVQESPNAIGGRGLSLGRIYRRDGVLLA
                     SVAQEGMVRVPST"
     gene            5722..6405
                     /locus_tag="PLW15_02735"
     CDS             5722..6405
                     /locus_tag="PLW15_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33347.1"
                     /translation="MTGTFTLVDSLALGDLHVFLGRALRVEDGSVRLIGGSGVLAVYV
                     SVLHPIGLLDESPTVLGLRTFALTSPEDFDVVVPVRSLLMRVEGLLESAAAADPTEGI
                     VVTIPMTVNTVTWAAISPPRGGWNPLPGADWNLLDRVARAGIDEVARAIPDGTGEQIV
                     HRVRTEIWGRPIDNLDFVPAGAAFAALALGFLAEGEAISIYESGPWTRLTTKRGHVLV
                     KRRAWSLAR"
     gene            complement(6416..6832)
                     /locus_tag="PLW15_02740"
     CDS             complement(6416..6832)
                     /locus_tag="PLW15_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOT33348.1"
                     /translation="MSDWNDKIIAEFRSNDGKVGGPFEGAHLLLLTTTGAKSGLARVS
                     PMVYFEQEGGLFVIASKAGAPTHPAWFHNLAANPQVSVEMATEHGIDTFTGTAVPVTG
                     ERRDALFASFSSRNPGFAQYQAKTDRVIPVVEISRG"
     gene            complement(6844..7470)
                     /locus_tag="PLW15_02745"
     CDS             complement(6844..7470)
                     /locus_tag="PLW15_02745"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT33349.1"
                     /translation="MSNSSTPSAAAGWYPDPAGSARSRWWDGTQWTEHYYDPATGVPV
                     AQLKAPEGTKVYNLWIWLVVFLPYITLPFLFLIDFSGMFSNMDPGNPSGVVRAELQIL
                     TSPGYVLLVVGGWIAFAGVVVSAYRDWRALQVAGVPRPFHWAWSFLNPVYAIGRAVVT
                     KRRTGHGSAVLWATIGMIVLSFVISTVWASMLMVNMMQQLSTYSTLYN"
     gene            complement(7509..9161)
                     /locus_tag="PLW15_02750"
     CDS             complement(7509..9161)
                     /locus_tag="PLW15_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HOT33350.1"
                     /translation="MSDSDALVIGAGLAGLVAAAELVAAGKTVTVLEQEPEASFGGQA
                     WWSFGGLFLIDSPEQRRMGIRDSYELARQDWFGSAGFDRAEDERPRQWAEAYLEFAAG
                     EKRSWLRERGVSFFPVVGWAERGGYTATEHGNSVPRFHITWGTGPGILEPFVRAVRAG
                     VDSGLVTLKFRHRVDELIVDHGAVVGARGSILAPDAAERGAPSNRNVTGDFQARAGAT
                     IVTSGGIGGNHDLVRERWPKRMGKAPEHLLSGVPAHVDGRMLGISERAGANLINGDRM
                     WHYVEGITNYAPVWPQHGIRILPGPSSVWLDATGRRLPVPLFPGFDTLGTLEHIVATG
                     HDYSWFVTTQAIIEKEFALSGSEQNPDLTGKSVRELLRQRLGKGATEPVEAFKSKGED
                     FIVGTTLDEILDGMQAKSPTVTIDRDRVRLEVEARDRELTNSFSKDAQINAIRQARHY
                     RGDRLIRVAKPHRILDPKAGPLIAVKLHILTRKSLGGIETDLSGQALDGSGAPIPGLY
                     AAGEASGFGGGGMHGYRSLEGTFLGGCLFSGRAAGRAVAQRG"
     gene            9332..>10164
                     /locus_tag="PLW15_02755"
     CDS             9332..>10164
                     /locus_tag="PLW15_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33351.1"
                     /translation="MTDATPPTRTPRWRRATGLTLIVLGALLAPVALVSNWAVVELTD
                     TDAFVDTFAPLAQDPEVQSLISGTVTRAITDQVDIPALTEPVFEGLGLNLGPVASAGV
                     GALQDVVTAAIHQLVGSTVERFVASDAFATVWEKALRVSHSQVIAALQNDGTVLTFDQ
                     GTVGIQVGPIVEAAKSALVEQGLGFAARIPEVDRTIVIVKSDALAQAQVAYAWAVGIA
                     GWLPWVALLLLVAGILIAGGSTFSMLFTAAGVSVTMVLLLVGLAVGRAVVIKEFVAAD
                     LP"
     assembly_gap    10165..10264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10265..10606)
                     /locus_tag="PLW15_02760"
     CDS             complement(<10265..10606)
                     /locus_tag="PLW15_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT33352.1"
                     /translation="MSVTTLRGPGFTDGVLPAACPTRTVLDHVTSKWGVLVIIALSEE
                     SLRWGELRRVVQGISEKMLAQTLRVLEADGFVLRVAQPTIPPRVDYSLTERGRELSAR
                     LLPLMAWIDDNA"
     gene            10701..11561
                     /locus_tag="PLW15_02765"
     CDS             10701..11561
                     /locus_tag="PLW15_02765"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT33353.1"
                     /translation="MTIVVTGATGHLGHLIVESLLSRGVPADQLVAVGRNLDKVADLV
                     ERGVAVAAIDYENPASLDATFAGADTLVLVSSSEVGKRATQHAAAIDAASRAGVGRVI
                     YTSAPAARTSALILAPEHKATEEYLEKSGLAHTVLRNGWYHENYVGSAAQAAASGTLA
                     SSAGEGTVASASRADYAEAAAVVATTDGHEGAVYELSGDRAWNFAELAATISSIAGTP
                     VSYAVLTPEEHHAALTGAGLDEGTAGFVVALDGNIRDGLLGSTSGDLSRLIGRPTTPI
                     ETTLAGALKG"
     gene            complement(11565..12008)
                     /locus_tag="PLW15_02770"
     CDS             complement(11565..12008)
                     /locus_tag="PLW15_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HOT33354.1"
                     /translation="MSGGHEPNPVTLRLSQSGESVQGNFWEQVGGRPTFEKLVRAFYD
                     GIRTDEVLLPMYPEQPDLEGAIQRLTGFLEQYWGGPTTYSEERGHPRLRMRHAPFKVN
                     PDARDRWLKHMRAAVDTLELSPLHDATLWAYLDRAAQAMVNTLDD"
     gene            complement(12005..13051)
                     /locus_tag="PLW15_02775"
     CDS             complement(12005..13051)
                     /locus_tag="PLW15_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOT33355.1"
                     /translation="MDWAEFWKQVGAFVGFWHVPLTVVAIIVGAVVLRWVLLRVIRRV
                     VNRVVSGVKKKHKVDDTRELAASPLAAVRVVQRTRSLGSVLGNATTGLIVLVAILLIF
                     YVTIPNATGAFALITAAVGAGLGFGAQNIVKDVLNGIFMVGEDQLGIGDVVDLGPATG
                     VVEDVGIRITQVRDVNGTLWFVRNGEILRLGNMSHGWARVIIDLAVPYDSDVDAIQDR
                     ILEVAVELSEDKNWRYRIIEKPSLWGIESISAEAIVVRVVVKTRSTAKDDVARELRYR
                     LKKALDDMGVKLPSLNSVMLSGFAGAAGLRSTKAQRPKPKPLIDPALQNRPNQKKPAT
                     KPVPTQTSSDKDDA"
     gene            complement(13061..>13718)
                     /locus_tag="PLW15_02780"
     CDS             complement(13061..>13718)
                     /locus_tag="PLW15_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HOT33356.1"
                     /translation="DALWALAQDADAASDTQFQFVKFFANIASTADHVATLSGLLDGS
                     ITLDGLEIDTDLRWELLEGLALNGATDAVVIDAALEKDNTSNGQQAAARAHATLPTAE
                     AKRAAFDSLVSKDDLPNVIVRNVTVGYLHTNDPSSLSGLVEPYFAMLNDIWTSRSYKI
                     AEYIVLGLYPAPLASQELVDATNAWLAANPDVPALRRLVVENLAGVERALKAQARDRA
                     "
BASE COUNT         2303 a         4546 c         4491 g         2178 t
ORIGIN      
        1 cgagatgctc tcgctgctca tccttccgac ggcggtggcg acattggctc cggcgatcgc
       61 cgagccgacc ggcagaagga tgagcagcga gagcatctcg gagatcggcg gccaacccgg
      121 ggtgagcgcg atgaccaccg accctgcagc acccggtatc aggaacgcga gcagcagcgc
      181 caccagcgtg ggtcggccga accgtatccg cgtgaagcgc agcaccgcca gagacagggc
      241 gagaagcagc accatcaccg agatggcaag gatggttcgc aacgcgaacc cgaacccctg
      301 aactcgcagc gtgtcgggca ccaccagggg cagcatcgcg acaccggcga gggccgacgc
      361 cagaagcgga cggtccgggg gaaagtcgag ggccgccgag atcggcgccc gctgaatgtg
      421 ggacacctgt gcctggtgct cgggcccgcg ggcagcgacc aggcgagagg tgtcgtacgc
      481 gacgctgctc accctcttgg ccatggccgt gaccgagctc cgagtcagcc ggggcgcgat
      541 cgtggtgaga ctcctgtgca actccgagat cacgggcccg atcgtggact gcacggtcgc
      601 gatgagatgc tcccgccgct cgcgagccga ctggtcgacg cgagcgcgtt ctgttgctcg
      661 cagcagccgc acggcggtga gttcgccgag cagggcgcgg cgatggtcga gctgggcgag
      721 cgtgtacgtg aacagcggac tccacaccag gctgatcgcc acccacacca gaacgcggta
      781 gccgacgtcg gcctccgtgt gaagcaccca ggcatgccag gcgctccaaa cgagtccgcg
      841 cacgacgccg acaccgaccc acatggcgaa gaccaagccg atcgggatga cgcgccagcg
      901 ccgccgcaga gcccagacga cagcaaagac cagcagtccg gcagcgaggt gttccagaac
      961 tgcgagaacg agtgccgcgg cccacccgga ggcgccgatc gtcgtctcct gaagaaccac
     1021 gagaaccgtg gtcggcacga acaggatgag ccagccgggg acgttaccgg cccacgggcc
     1081 gcccatggcc tcgcgaacgc tcagcacgtg cacctcacca ccttagccag cgatcaccag
     1141 gtctgtccga gatggggcgg gccgcttctt ctccaacccg aatggagaag aagcggtccg
     1201 tagagtgcac caacccgttc gctgatcccc cgatcccgcg ttccctctgc ggttgcgatg
     1261 ggcgatgctg cttacccgtg atcattctct gcggcccgaa tggccggggc aaccggccgg
     1321 gccgtggggg tattcgcatt gcgaacatcg cccgaacctc tccgcgggtg actcgatgtc
     1381 agcggggcat ccccagatcc ttcccccgcc tcgggcgagc tgtgtcgtgg tgctcagact
     1441 ggtgcaacgg cccccggtgc agcgtcccgg cacagggctc accggggacc gtccactacc
     1501 gaaggagaca cacatgactg acaccatcac cctcaccgga ctcgtcgcca cagacccgcg
     1561 ccatgtcgtc acgagcgagg ggctcgcgat caccagcttc cgtctcgcgt caacgcagcg
     1621 acgattcgat cgcagtcagg agaagtggat cgacggcgac accaactggt acacgatcac
     1681 gacattccgc cagctcgcga tgaacagtgc gggctccgtc aagaagggcg agcggattgt
     1741 cgtgaccggc cggcttcgca ttcgcgagtg gcagagcggc gagcgcacgg gactcaacat
     1801 cgatgtcgag gcggatgccc tgggtcacga cctggcgtgg ggtacctcca ccttcagccg
     1861 gagcatcagc gccacaacgg cttctgccga ttcgggctcg gctaccgccg acgaaccgac
     1921 tgcggatgag ccgtcggccg atgccgacga gacgtccgcg cagtccgacg agtcgcgtcc
     1981 catggctacg ctgccattct gaccggttcc gcaggttgct gaccctagaa tcactgtgcg
     2041 gatcgcaccg gcgggccgcg gaaggacgca tcgatgccac tgcggtacac ccgtcacctc
     2101 gcggcgatcg cgggtgttgt ggcgctcggc gcggtgctcg cgggatgtgg cggcgcaccc
     2161 atgccgacac cgaccgcgag ttcgtcacgc agcgcgaccc cctcgccaag tcccactgcc
     2221 aaggctcccg tgttccaccc ggacggctcg gcggcggcaa accagcagta cttcgactat
     2281 gtcaatcgcg cactgaacgc gtcatccggt ctgtccgacg gccgcacaat cgtggataac
     2341 ctcgtggctg ctgggttcac caaggccgac atggaggtga cggccgacac gaccgcgctc
     2401 gacctcacag tcgactcgat tcaggtggcc gtgcgcatca agggggagtg cctcatcggg
     2461 ggattctcct acgtgggcta tcagggcatc gttgcgccac ttctcggcag cggcacgtgc
     2521 ctcgtgggag cgacccgccc gatcgactgg taggcctccg gcgctcccta caatggagca
     2581 atggccgaat tcatttacac catggtgcgc gcccgcaaag cggtcggcga caagctcatc
     2641 ctcgacgacg tgaccatgtc gttctacccg ggggcgaaga tcggtgtggt cggcccgaac
     2701 ggtgctggta agtcgacgat cctcaagatc atggctggtc tcgacactcc gtccaacggg
     2761 gaagcccgtc tgacccccgg cttcagcgtc ggcattctca tgcaggagcc cgaactggac
     2821 gagtcgaaga ccgtcctcga gaacgttcag gagggtgtcg ccgagatcaa ggacaaggtc
     2881 gaccggcacg ccgcgatcgg gctcgagatg gcggagcccg acgccgactt cgatgccctc
     2941 ctcgccgaga tgggaacgct ccaagaagac atcgatgccg ccgacgcctg ggatctcgac
     3001 tcccagctcg aacaagccat ggatgccctg cgcaccccgc ccggcgacgc aaccgttgcc
     3061 aacctctcgg gtggcgagaa gcgccgtgtc gccctgacca agttgctgct gcagaagccc
     3121 gacctgctgc ttctggatga gcccaccaac cacctcgacg cggagagcgt gctctggctt
     3181 gagcagcacc tggccaagta ccacggtgcc gtactcgccg tcacccacga ccggtacttc
     3241 ctcgaccacg tcgccgagtg gatcgccgag gtcgaccgcg gccacctcta cccctacgag
     3301 ggcaactact cgacctatct gcagaagaag ggcgagcgcc tcgaggtgca gggcaagaag
     3361 gacgcgaagc tcgccaagcg cctcgcctcc gagctcgagt gggtgcgcag caacgcgaag
     3421 ggccgccagg tcaagtccaa ggcacgcctc gcccgctacg aggagatggt caacgaggcg
     3481 gagaagaccc gcaagctcga tttcgaggag ctggtcatcc cggtcggacc gcgcctcggc
     3541 tcgcaggtca tcgacgcgaa gaatctgaag aagggcttcg gcgagcgcgt gctcatcgac
     3601 aacctcacct tcacgctgcc ccgcaacggc atcgtcggcg tcatcggtcc caacggtgtc
     3661 ggtaagacca ctctgttcaa gacgatcacc ggtctcgagc cgctggactc gggcgacctc
     3721 aagatcggcg agaccgtcga catctcgtac gtcgaccaga accgcggcgg catcgacccc
     3781 aacaagaccc tgtgggaggt ggtgtcggac ggccaggact tcatccaggt cggcaagacc
     3841 gagatcccgt cgcgcggcta tgtcagccag ttcggtttca agggacccga ccagcagaag
     3901 aaggcgggcg tgctctccgg cggggagcgc aaccgtctca acctcgccct cacgctcaag
     3961 cagggcggca acctccttct gctcgacgag ccgacgaacg acctcgacgt cgagaccctg
     4021 ggcagcctcg agaacgcgct gctggagttc cccggttgtg cggtggtcat cactcacgac
     4081 cggtggttcc tcgaccggat cgccacgcac atccttgcct acgagggcac cgaggatgac
     4141 ccggccaact ggtactggtt cgagggcaac ttcgaggcct acgaggagaa caagatcgag
     4201 cgcctcggac cggatgccgc aaagccgcac cgcagcgcct atcgcaagtt gactcgggan
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gacccgactg cacgtcccca
     4381 tcccgctgcg gtggagcgat ttcgacgcgt acgcccacgt gaacaacgcc gagatgctgc
     4441 gcctgctcga ggaggcgcgc atccaggcat tctggcgtcc cgacgaggga agtgacggcg
     4501 gcgcggtgac ggccgttctc gatgctcgcc cgggtgccga gacgatcagc ctcatcgccc
     4561 ggcaggagat cgagtatctg gcaccgatcc cgtacatgcg cgctccgatc gacatcgaga
     4621 tgtggatcgg ccgtatcggc ggtgcgagcc tggaaatctg ctacgagctg ttctcgcccg
     4681 tgggcgtgac gccacgggtg ctgttcacgc gagcggcaac cacgctggtc atggtcaccg
     4741 ccgccacggg taagccgcag cgaatcggtg acgagcttcg cgccctctgg cagccctatg
     4801 tggagccacc cgtgcagttc accaagcgcg gttaggtgct cggcacccgg accatgccct
     4861 cctgcgcgac actggccagc agcactccgt cgcgccggta gattcggccc agcgagagcc
     4921 cgcgtccgcc gatggcgttc ggggattcct gtacgtagag caaccactcg tcgacgcgcc
     4981 ccggcctgtg ccaccacatc gcgtgatcga ggctcgcggc cttgagaccg ggcatggccc
     5041 acgcgatccc gtggcgccgg aggatcggct cgaggatcga gtagtcgctg gcgtaggcga
     5101 gcgctgcgcg gtgcaggttg tcgtcgtcgg gcagcctgcc gacggccttc agccacacgg
     5161 cctggtgcgc gaccctgtcg ccctcgacgg agaggtagac cggtgacggc acgtgtcgca
     5221 tgtcgaaggg gcgttcggtg gcccagaagc gagcaacggc gtggtcaatc tcgcccaggt
     5281 gctcagctgc gctgggaaga ctctccgggt cggggatgcc cacgggcatg tcgacctgat
     5341 gctctagccc ctcatcctca tcctggaacg acgcgatcat ggagaggatc ggcaagccct
     5401 cctgatatgc ctgagtacgg cgggtggaga acgagcgtcc gtcgtggatg cggtctactg
     5461 agaacgtgat cggcaggtcc acctgccccg gacgtaggaa gtacgcgtgc atcgagtgga
     5521 cgacgcgccc gtcagggctc gtgcgctgcg ctgctaccag cgactgggcg agcacctgcc
     5581 ctccgaacac ccggccgtgg ggctgccaga ggctcggacc ggtgaagatg tcctcactcg
     5641 tgcgggcacc ggtgtcggtg aggtcgagcg ctgcgaggaa gccggacagg gggtcgctca
     5701 tgccctgtag tttaggagac tgtgactggc accttcaccc tcgtggactc tctcgccctg
     5761 ggcgacctcc atgtgttcct cggccgggcg ctgcgcgtcg aggatggttc ggtgcgtctg
     5821 atcggtggct cgggtgttct ggcggtctac gtctcggtgc ttcatccgat cgggcttctc
     5881 gacgagagcc cgaccgtgct cggcctgcgc acgttcgcct tgaccagccc agaggacttc
     5941 gacgtcgtcg tgcccgtgcg atcgctgctc atgcgcgtcg aggggctgct cgagtccgcg
     6001 gcggcggccg atccgaccga aggcatcgtc gtgacgatcc ccatgaccgt caacacggtc
     6061 acctgggcgg cgatctctcc accccgaggg ggatggaacc cacttcccgg cgccgactgg
     6121 aacctcctcg accgggtcgc gcgtgcggga atcgacgagg tagcaagggc gatcccggat
     6181 ggcacgggtg agcagatcgt tcatcgcgtg cgcaccgaga tctgggggcg ccccatcgac
     6241 aacctcgact tcgtgccggc gggggctgct ttcgctgctc tggcactcgg attcctcgcc
     6301 gagggtgagg cgatctccat ctacgagtcg ggcccgtgga ctcgcctgac caccaagcgt
     6361 ggtcacgtgc tggtgaagcg tcgggcgtgg tccttggctc gctagcagca gctcgctagc
     6421 ctcggctgat ctccaccacc gggatcaccc gatccgtctt cgcctggtac tgcgcgaagc
     6481 cagggttgcg gctggagaag ctggcgaaca gcgcgtcacg gcgctcgccc gtcacgggaa
     6541 ccgcagtacc cgtgaacgtg tcgatcccgt gctcggtcgc catttcaacg ctgacctgcg
     6601 gattcgctgc gaggttgtgg aaccacgcgg gatgcgtcgg ggcgccggcc ttcgacgcga
     6661 tcacgaagag cccaccttct tgctcgaagt agaccatggg tgacacgcgg gccagcccac
     6721 tcttcgctcc cgtcgtggtg agcagcagga ggtgcgcgcc ctcgaaggga ccaccgacct
     6781 tgccgtcgtt cgagcggaat tccgcgatga tcttgtcgtt ccagtcggac actcgggcca
     6841 gccttagttg tagagagtcg agtaggtcga gagctgctgc atcatgttca ccatgagcat
     6901 gctcgcccag accgtggaga tgacgaacga gagcacgatc atgccgattg tggcccacag
     6961 caccgctgag ccgtgcccgg tgcggcgctt ggtgacgact gccctgccga tcgcgtagac
     7021 ggggttgagg aagctccacg cccagtggaa ggggcgaggc acccctgcga cctggagcgc
     7081 acgccagtcg cggtaggcgg acaccaccac accggcgaac gcgatccacc cgccgaccac
     7141 caggagcacg tagccggggg aggtgaggat ctgcaactcg gcgcgcacaa cgccactggg
     7201 gttgccggga tccatgttcg agaacatgcc gctgaagtcg atcaggaaca ggaacggcag
     7261 ggtgatgtag ggcaggaaca ccacgagcca gatccagagg ttgtagacct tcgtgccctc
     7321 gggcgccttc agctgggcca ccggaacgcc cgtcgccggg tcgtagtagt gctcggtcca
     7381 ctgcgtgcca tcccaccacc ggctgcgtgc cgaaccggca gggtcgggat accagcctgc
     7441 ggccgcagag ggcgttgacg aattcgacat gggaactcct tcgctcgggg caatcctacg
     7501 agccgtcgtc acccgcgctg cgccaccgcg cgaccggcgg ctcttccgga gaagaggcat
     7561 ccgcccagga aggtgccctc gagtgagcgg tagccgtgca ttccaccgcc cccgaagccg
     7621 ctcgcctcgc ccgccgcgta gagtccggga atcggtgcgc cgctgccatc gagggcctga
     7681 cccgacagat cggtctcgat gccgccgagg ctcttgcgag tgaggatgtg cagcttgacg
     7741 gcgatcagcg gaccggcctt cggatcgaga attcgatggg gcttcgccac ccggatcaac
     7801 cggtcgcccc ggtagtggcg ggcctgccgg atcgcgttga tctgggcgtc cttgctgaag
     7861 gaattggtga gctcgcggtc acgcgcttcg acctcgagtc tgactcgatc ccgatcgatc
     7921 gtcaccgtcg gggacttcgc ctgcatgccg tcgaggatct catcgagagt ggtgccgacg
     7981 atgaagtcct cgcccttgct cttgaacgcc tcgaccggct cggttgcgcc cttgcccaga
     8041 cgttgccgca gcagctcgcg cacactcttg ccggtgaggt cggggttctg ctcgctgccg
     8101 gagagggcga actccttctc gatgatcgcc tgggtggtga cgaaccacga gtagtcgtgt
     8161 ccggttgcga cgatgtgctc gagcgtgccc agcgtgtcga agccggggaa caggggaacg
     8221 ggcaggcgtc ggcccgtcgc atccagccac acactcgacg gcccgggcag gatgcggata
     8281 ccgtgctgcg gccacacggg tgcgtagttg gtgattccct cgacgtagtg ccacatgcgg
     8341 tcgccgttga tcaggttcgc gccggcgcgc tcgctgatgc cgagcatgcg gccgtcgaca
     8401 tgcgcgggca cgccgctcag gaggtgttcg ggcgcctttc ccatgcgctt gggccagcgt
     8461 tcgcgcacga ggtcgtggtt gccgccgatg ccgccgctgg tgacgatggt cgccccggcg
     8521 cgagcctgga agtcaccggt gacattgcgg ttgctggggg ctccacgctc ggccgcatcg
     8581 ggcgcgagga tcgagccgcg agcacccacc accgctccgt gatcgacgat caactcatcg
     8641 acgcgatgcc ggaacttcag cgtcaccagt cccgaatcga cgccggcgcg cacggccctg
     8701 acgaacggct cgaggatgcc cggcccggtg ccccacgtga tgtggaatct cggaaccgag
     8761 ttgccgtgct cggtcgcggt gtagcccccg cgctccgccc agccgaccac cgggaagaag
     8821 ctcacgcccc gctcacgaag ccaggaacgc ttctctcccg ccgcgaattc gaggtacgcc
     8881 tcagcccact ggcgaggccg ctcgtcctcc gcccgatcga agcccgcact gccgaaccag
     8941 tcctgccgcg cgagctcgta gctgtcgcgg atgcccatcc ggcgctgctc gggggagtcg
     9001 atgaggaaca gcccaccgaa cgaccaccag gcctggccgc cgaagctcgc ctccggctcc
     9061 tgctcgagca cggtgaccgt cttgcccgcc gcgaccaact cagcggcggc gacgaggccc
     9121 gccagtcccg ctccgatgac taacgcgtcg gaatcagaca tcagggcact ccttcgtggc
     9181 cggcgtggat gctctagctt aggcacgtga ggcacgcagt cgaggttgcg cagggccggt
     9241 cgctgcggcg cgacatcccc cgctcgcgga cttcgaacag gtgcgcgcgc gggcactaac
     9301 ttaggaaagc cgcagccgaa gggacgatga catgaccgac gccacacccc cgacccgaac
     9361 gccccgctgg cggcgggcga caggtctcac cctcatcgtg ctgggcgcac tcctcgcccc
     9421 cgttgccctg gtgagcaact gggcagttgt ggagctgacc gacaccgacg cctttgtcga
     9481 caccttcgcg ccgctcgcgc aggatcccga ggtgcagagt ctcatctcgg gcacggtcac
     9541 gcgggcgatc accgaccagg tggacatccc cgcgctcacg gagccggttt ttgaggggct
     9601 tgggctgaac ctcggccccg tggcatccgc tggggtgggc gcgcttcagg acgtggtgac
     9661 ggcggccatc catcagctcg tcggctcgac agtcgagcgc ttcgttgcgt cggatgcctt
     9721 cgccacggtc tgggagaagg ctctgcgggt ctcgcactcg caggtcatcg ccgcacttca
     9781 gaacgatggc acggtgctca ccttcgatca gggcaccgtg ggcatccagg tgggaccgat
     9841 cgtggaggcc gccaagtccg cgctcgtcga gcagggactc ggcttcgctg cgcgcatacc
     9901 cgaggtcgac cgcacgatcg tcatcgtcaa gtccgacgcc ctcgctcaag cgcaggtggc
     9961 gtacgcctgg gccgtgggca tcgcgggttg gctgccctgg gtcgccctgc tgctgctcgt
    10021 cgcgggcatc ctgattgcgg gcggctcgac gttcagcatg ctgttcaccg ctgcgggggt
    10081 gagcgtgacg atggtgctgc tgctcgtggg gctcgcggtg ggccgcgcgg tcgtcatcaa
    10141 ggagttcgtc gcggccgacc tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnggcgtt gtcgtcgatc cacgccatca gcggcagcag gcgcgccgag agctcgcggc
    10321 cgcgctccgt gaggctgtag tcgacacgcg gcgggatcgt gggttgggcc acgcggagca
    10381 cgaagccgtc ggcctcgagc acgcgcaggg tttgggcgag catcttctcg ctgatgccct
    10441 gcaccacccg acgcagctca ccccagcgca ggctctcctc gctcaacgcg atgatgacga
    10501 gcacgcccca cttgctcgtc acgtggtcga gcaccgtgcg ggtgggacag gcagcaggca
    10561 ggaccccgtc ggtgaagccg ggcccgcgca gggtggttac gctcatgcaa ctaacttacc
    10621 aaaaggtggg tactgtctgt cgggaagcat tgctgaatcg acgcggttgg cagtagggat
    10681 cacagaaagg atcaccacac atgacaatcg tcgtcaccgg cgcgaccgga cacctcggcc
    10741 atctcatcgt cgaatccctc ctctcacgcg gagttcccgc ggaccagctg gttgcggtcg
    10801 gcaggaacct ggacaaggtg gccgacctcg tcgaacgcgg cgtcgccgtc gcggcaatcg
    10861 actacgagaa tcccgcgtcg ctcgatgcga ccttcgcggg ggcggacacc ctcgtgctgg
    10921 tgtcgtcgag cgaggtgggc aagcgggcca ctcagcacgc agcagccatc gacgctgcca
    10981 gccgcgctgg cgtcggtcgg gtgatctaca cgagtgcacc ggccgcgcgc acctcggcac
    11041 tgatcctcgc gcccgagcac aaggccacgg aggagtacct ggagaagtca gggctcgcgc
    11101 acacggtgct gcgcaacggc tggtaccacg agaactacgt gggcagcgcg gcgcaggccg
    11161 cggcatccgg aactctggca tcgagcgcgg gtgagggcac cgtcgccagc gcctcccgcg
    11221 ccgactacgc ggaggcggcg gcggtcgtcg cgaccaccga cggacacgag ggcgccgtgt
    11281 atgaactctc cggcgaccgc gcgtggaact tcgccgaact cgccgcgacg atctcgtcga
    11341 tcgcgggcac gccggtgtcg tacgcggtgc tgacccccga ggagcaccac gcggccctca
    11401 ccggcgcagg cctcgacgag ggaacggctg gcttcgtggt cgccctcgac ggaaacatcc
    11461 gcgacggact gctcggaagc acgagcggcg acctctcgcg cctcatcggt cgacccacta
    11521 ccccgatcga gacgaccctc gcgggcgcgc tgaagggcta gcgctcagtc gtcgagggta
    11581 ttgaccatcg cctgcgcggc gcgatccagg taggcccaca gggtcgcgtc gtgcagcggt
    11641 gacagctcga gggtgtcgac tgccgcacgc atgtgcttca gccatcgatc gcgcgcgtcg
    11701 gggttgacct tgaaaggggc gtggcgcatc cgaagccgcg gatgcccgcg ctcctcgctg
    11761 tacgtcgtgg gcccgcccca gtactgctcc aggaatcccg tcagacgttg aatcgcgccc
    11821 tcgagatcgg gctgctccgg atacatcggc agcagcacct cgtcggtgcg gatgccgtcg
    11881 tagaaggcgc gcaccagctt ctcgaacgtg ggtcgtccgc ccacctgctc ccagaagttg
    11941 ccctgcaccg actcgccgct ttggctcagg cgcagcgtca cagggttggg ctcgtgtccg
    12001 ccgctcatgc gtcgtccttg tctgaggaag tttgggtggg caccggcttg gtggctggct
    12061 tcttctggtt ggggcggttc tgcaacgccg gatcgatcaa cggcttcggc ttgggtcgtt
    12121 gcgccttggt gctgcgaagg cccgccgcgc ccgcgaatcc ggagagcatg accgagttga
    12181 gtgatggcag cttcacgccc atgtcgtcga gcgccttctt gagccggtag cgcagttcgc
    12241 gcgcgacgtc atccttcgcg gtcgaccggg tcttgacgac cacgcgaacg acgatcgctt
    12301 ccgcggagat cgactcgatg ccccacagcg atggcttctc gatgatgcgg tagcgccagt
    12361 tcttgtcttc ggacagttcg acggcgacct cgaggattcg atcctggatc gcgtcgacat
    12421 cggagtcgta cggcacggcg aggtcgatga tcacccgggc ccagccgtgg gacatgttgc
    12481 cgagtcgcag gatctcgccg ttgcgcacga accagagggt gccgttgacg tcgcgcacct
    12541 gagtgattcg gatgccgaca tcctcgacga caccggtggc cgggccgagg tcgacaacgt
    12601 caccaatgcc caactggtct tcgccgacca tgaagatgcc gttgagcacg tccttcacga
    12661 tgttctgcgc accgaaaccg agaccggcac cgacggcggc cgtgatgagg gcgaacgcgc
    12721 ctgtcgcgtt ggggatcgtc acgtagaaga tcagcaatat cgcgacgagc acgatgagac
    12781 cggtcgtcgc gttgcccagc acgctaccga gtgatcgggt gcgctgtacg acccgcaccg
    12841 ctgcgagcgg cgaggccgcg agttctcgag tgtcgtcgac cttgtgcttc ttcttgaccc
    12901 cgctcaccac ccgattgacg acgcggcgga tgacgcgcag gagcacccac cgcagcacca
    12961 cagcgccgac gatgatcgcc acgacggtca gcggaacgtg ccagaagccg acaaaggctc
    13021 cgacctgctt ccagaattcc gcccagtcca ccggatgccc ctacgcgcga tcccgagcct
    13081 gcgccttgag ggcgcgctcg acgccggcga ggttctccac cacgaggcgg cgcagtgccg
    13141 gaacgtcggg gttcgccgca agccaggcgt tcgtggcatc caccagctcc tgactggcca
    13201 gaggcgccgg gtacagaccc agcacgatgt actccgcgat cttgtagctg cgcgaggtcc
    13261 agatgtcgtt cagcatcgcg aagtacggct cgacgaggcc cgagaggctc gacgggtcgt
    13321 tggtgtgcag gtagcccacc gtgacgttgc gcacgatcac gttcggaagg tcgtccttgc
    13381 tcacgagcga gtcgaacgcc gcgcgcttcg cctcggccgt cggcaacgtc gcgtgggctc
    13441 gcgcagccgc ctgctggccg ttggacgtgt tgtccttctc cagtgccgcg tcgatcacca
    13501 ctgcgtcagt tgcgccattg agcgcgaggc cctcgagcag ctcccagcgc aggtcggtgt
    13561 cgatctccag gccgtcgagg gtgatggatc cgtcgagcag gccgctgagg gtggcgacat
    13621 ggtcggcggt cgaggcgatg ttcgcgaaga acttcacgaa ctggaactgg gtgtcggatg
    13681 ctgcatcggc gtcctgtgcg agcgcccaga gcgcgtcg
//
LOCUS       DAOSOU010000029        13293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4692_length_13293_cov_7.119958, whole genome shotgun sequence.
ACCESSION   DAOSOU010000029 DAOSOU010000000
VERSION     DAOSOU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_13293_cov_7.119958"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLW15_02785"
     CDS             complement(<1..408)
                     /locus_tag="PLW15_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33357.1"
                     /translation="MPQEDETLSLEFRDYLALCAIELSKVPARIAISDSVSDCEVAHV
                     FLMHGLPDALCMVIKRPLEPVRESAEVHGLEAFVETHDPKFPTLAIDLIVEEVEVDAE
                     ARLDDHRSRLEVSKNALWPLGWIEEQHQELQLWR"
     gene            634..867
                     /locus_tag="PLW15_02790"
     CDS             634..867
                     /locus_tag="PLW15_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HOT33358.1"
                     /translation="MHTSALRHGVSAEDAILAATEYVVKAELAEDRELRLGFDSAGRL
                     LETVVVILSSGQQAVIHAMPARRTFLALLERKT"
     gene            864..1154
                     /locus_tag="PLW15_02795"
     CDS             864..1154
                     /locus_tag="PLW15_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOT33359.1"
                     /translation="MTHVTINGVQLEGVTVGGVPVTDAIVEKWADEAEHGYDIATLKF
                     RGRPRMGSEIAKVTTLRLEPALDVALSERAAKDGSSRSEVIRAALRAWLESA"
     gene            complement(1212..1646)
                     /locus_tag="PLW15_02800"
     CDS             complement(1212..1646)
                     /locus_tag="PLW15_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HOT33360.1"
                     /translation="MPTESHLLDLNVLVALAWPQHVHHRRAHTWFAARRGDWMTTPVT
                     ESGFVRLSTNPTVVTERISMADALALLAAMRAVPGHQFLPDASSLAQPAVSLAALATT
                     RQVTDAHLVNLAASSGVLLATFDRGIEKMLAAGDRRHVLVLP"
     gene            complement(1636..1866)
                     /locus_tag="PLW15_02805"
     CDS             complement(1636..1866)
                     /locus_tag="PLW15_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOT33361.1"
                     /translation="MRTTLDLDDTVIAVARAIARDEGISLGAAVSRLAERGLKAAGDS
                     STTGQSGFPVFTVSADAAPITLDMVNDQRDAD"
     gene            complement(1890..2297)
                     /locus_tag="PLW15_02810"
     CDS             complement(1890..2297)
                     /locus_tag="PLW15_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT33362.1"
                     /translation="MGLIYLDSCMLIYAVEDQGRRGETVRRHLSSSRNRVAISPLVVL
                     ECMVGPLKRDDLALRDQYANAIARCELLSLGLPEFLRAAELRARHALRTADALHLAAA
                     QLHGCDALWTNDSRLAAASSGLAVDRVTASIAE"
     gene            complement(2288..2542)
                     /locus_tag="PLW15_02815"
     CDS             complement(2288..2542)
                     /locus_tag="PLW15_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOT33363.1"
                     /translation="MEPVNILDARNRLSQLITAATNGEDVVIAKRGRPVVRIVPIVDD
                     AANTGEALVQWLQEHPLPAGAVRSAEELDEQIAREREAWD"
     gene            2690..3154
                     /locus_tag="PLW15_02820"
     CDS             2690..3154
                     /locus_tag="PLW15_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33364.1"
                     /translation="MTDVMKARSDAEFIGLIQELAGYTATNSLVCIAVHGLHYYSDFR
                     LDLPHRKRTADCRELALEAIGILSGMSRIDRVAIVIYTDETFAAHHGLPWLEFYTAFA
                     ERVHKQGFHFSGFFCIAADGWADYFDRDYPRQGRALSEIPSGPVELPQWASE"
     gene            3190..3426
                     /locus_tag="PLW15_02825"
     CDS             3190..3426
                     /locus_tag="PLW15_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT33365.1"
                     /translation="MRITSKGQVTIPQRLRERYGLMPETEVEFVEVGGEIVVRRAGAQ
                     SADRAQQIVDRMLGAGHSPLTTEEIIAIARGYDD"
     gene            3410..3838
                     /locus_tag="PLW15_02830"
     CDS             3410..3838
                     /locus_tag="PLW15_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT33366.1"
                     /translation="MATTTEVPVGESFLIDSNVLLDILGAGPWAAWSQDVVAACLRAG
                     PVHINPVVYAEISMTFANPIELDALVPPSVFRRLSIPYPAAFLAAKAHQAYRRRGGSR
                     AATLPDFFIGAHALVDGLTVVTRDPRRYRDAFPQVRVVAP"
     assembly_gap    4033..4132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4133..6052)
                     /locus_tag="PLW15_02835"
     CDS             complement(<4133..6052)
                     /locus_tag="PLW15_02835"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOT33367.1"
                     /translation="MSSNSTATTTSAPVQIKWWVKGDWNGLFGLGTNVLLNVIVLSGL
                     CLAVIQMPDNVVFGRILPALGIALPLGNIWYAILARRLARRENRTDVAALPYGPSVPH
                     MFIVVLVVMLPVFLQSGDPLKAWHAGLAWAFIVGFIILLGAIFGPWIRKWTPRAAMLG
                     ALAGISITFISMSPAAQMWEAPWIAFVTFGFILVGWLGFRKMPFNAPVGLVAVVAGTV
                     IAWIAVLLGWSDILKPSAVAESIASIGIYFPIPGGEVLAGLQDIAPLLATAIPLGIYN
                     FTEAMTNVESAAAAGDRYSARAVLTADGLGAIIGSFLGSPFPPAVYIGHPGWKKVGGR
                     IGYSLVTGIVVALVCFSGLVGLFLAIFPMQALVPILLFIGLVIGAQATNVSALRYAPA
                     IILAMLPSLAQWASGLIDNSLGAAGTNAAEVGLDKLAGNGVIYQGLTLFGSGAVLAGV
                     LLGSIAVFVIDRRMYAAAVTSGICMVLSFFGLINAVQVGINASPGVSLGYAFLMILLL
                     GFGWAARKDKEVVLQDDLLFVNGTLMRGLGLHGNLQGAEFIAEMKTAPKYRMHDIGGV
                     HPGMYEVEKDGVAVSGELYTLPIDVLLRVIENEPPGLYRGPVELADGRIVPGVLYDEA
                     MAKKHPVIESGNWRDH"
     gene            complement(6049..6759)
                     /locus_tag="PLW15_02840"
     CDS             complement(6049..6759)
                     /locus_tag="PLW15_02840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOT33368.1"
                     /translation="MNETTGTVTVPAEPYGFTFDPKKVALLCIDFQRDFMETGGFGES
                     LGNDVSTLRGSIKPTRAVLDMFREKGWMVIHTREGHREDLADLFPAKRDRGNPTLRIG
                     DDGPMGRLLTRGSAGHEIIPELAPIEGEVVLDKPGKGAFYATDLETILRAKGIEQLII
                     TGVTTEVCVQSTAREANDRGFECLILSDCTGSYFAEFYESALNMFKAQGGIVGWVGTS
                     TSLLDAVDSATTEIGAKK"
     gene            complement(6775..7746)
                     /locus_tag="PLW15_02845"
     CDS             complement(6775..7746)
                     /locus_tag="PLW15_02845"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOT33369.1"
                     /translation="MTATNDNTVLIAIGGNALVLDGEPGSVDRQLERAATFAESVAAL
                     VVDGWTAVVTHGNGPQVGYILRRGELVAPDAMVEGLPALPLWLAVADSQGGIGHMLAV
                     AIDSALDARHSGIRAVAVLTHAEVDADDPAFEKPTKPIGGQLTAEVARLRVAEEGWVV
                     TEMGENTYRRVVASPIPKTIIEASQIRTLVDSGAVVIAAGGGGIPVVRDSAGWHPVDA
                     VIDKDRASALLAGQIGVGTLVLVTGVDEVYVDFNAPGQRALRTVGVDELRDHLAAGQF
                     PPGSMGPKVASAIDFIEAGGTRAIITSISKLPDALAGTAGTIVTKNG"
     gene            complement(7743..9140)
                     /locus_tag="PLW15_02850"
     CDS             complement(7743..9140)
                     /locus_tag="PLW15_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOT33370.1"
                     /translation="MSILTDELSVINAGLPLIVEGVPAAQVTQLDWRPPAFGDADVAR
                     LALTLDNEVTDAANAVAIAKVHAVKPQLTRIRPAREVMPGIAEGRTLLHAGPPIEVER
                     MCGPMRGALIGATLFEGWADNADDALRMLDSGEIAVAPCHHHGAVGPMAGVIAPSMPV
                     LVATDLDDGRQAFASLNEGLGKVLRFGAYDSEVITRLTWMRDVLGPVLDRTLEKYGPI
                     DVTSLIGQALSMGDEGHNRNVAATSLLARRLAPTIAELEPNSDSVAVLAFLAGNDHFA
                     LNISMAAAKLALDAAIGVENSTLVTAMARNGVEFGLRVAGSGTEWFTTPVGPADGLFF
                     PGFGPEDANPDLGDSAITETLGLGGFAMAASPAITQFVGGTPNDALAATRSMRRITLG
                     PHPAYPLPPLNFAGTPSGIDVLKVLDTGVLPLINTGIAHKEAGVGQIGAGIVTAPSEV
                     FLKAARFLAEQRAHT"
     gene            complement(9137..10594)
                     /locus_tag="PLW15_02855"
     CDS             complement(9137..10594)
                     /locus_tag="PLW15_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33371.1"
                     /translation="MSDTVLSSRVLPHFYRDSVALMAIASTTEKRDGVVRVGAVMATP
                     ANLGILADSGMLPDGLTAAPDDLVFVVRGTDSEIVTAALDAAEAGLTAVDAGSATEEQ
                     RPGTIDEGVSVAALAEHPANLVAISIAGTYAPVVAEQALRGGMHVLCFSDNVAVADEV
                     RLKTLAVEKRLLMMGPDCGTAILDGVPLGFANAVARGSVGIVAASGTGAQEVSRLLDG
                     AGAGVSQLIGVGGRDLSAEVGGLMANLALDFLIDDPDTTVIMMVSKPPAPEVADALLA
                     RLAGIAANGTPVVACFLGQDDSDTDGVLVRGTLEGGALAAAGLAGHPFTVTEPEPVHA
                     PPGRVLGLYTGGTLASESKIILKRAGVVAEIIDLGDDEYTAGKPHPMIDPESRAEKIA
                     AAGSDATVGTILLDLVLGYGANTDPATPVATAVAQAKAAAAADGRTLLVVGSVCGTAG
                     DPQGLARQRGILTSAGVVLAETNAAAARLAAGGVA"
     gene            complement(10591..11427)
                     /locus_tag="PLW15_02860"
     CDS             complement(10591..11427)
                     /locus_tag="PLW15_02860"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HOT33372.1"
                     /translation="MIAEPDLARIDATLGASAADGFSAGYQLIVHLGQSVVVLRSTTT
                     QRLLTIANASGGVTPGGVCIPNGAEFARVRRELSAVAPGHPVDLSAWSRTATLVPVDL
                     HITPRSLDAGAIHAVSAELARGNASPAASFDGGLARVRSLAPALAEAAIHGDPRVLLP
                     ELIGAGPGTTPSGDDMVVGTLAALAALGLADAASRLSAAVTPLLGATTAASRHYIVAA
                     ASGRFAEHVHQLVDGLVDGMPARTILACASRWGATSGVDLLTGLAATLRAELAHRTKG
                     AA"
     gene            11539..13038
                     /locus_tag="PLW15_02865"
     CDS             11539..13038
                     /locus_tag="PLW15_02865"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOT33373.1"
                     /translation="MSTITVREVLTLPVLRGTFVVAGQRGLSRAVTGVNVMEVPDIES
                     FVKGGELLLTTAYPLREHPEDLSTLVRTLSRLGLAGLAVKTGRYLEKLPDEALAVANE
                     LDFPVMLVAEHTSFNEVIGAVLAVVLTEYGAEPAGAEAIRERLTGVALAGGGLVEIAR
                     TLAGALGRHVGILDPDGALLGEGGGGERAGNDAAHEPWSFPITVGGTERGRIVVRGDD
                     EPTLGQRRLIRQACFAAGMHIAQAVASLELDRQMRVLFLEELLAGKTIDESLARERSR
                     LFGWDFGSDHVVVLARLSHELADATVARAAETGFIRGAVAWARGQEAVAIVPAASIPG
                     DGTPWVEAAHWGETLARLGGGKVTVATGSIAESPSRLAESHSAARSTLAIAQATGRAN
                     ARHDLLALERVILSVPDDLLAELVAAELGPLIAVDAATGSELCETLEMFLGTGNAADA
                     ARRLFIHYNTMKHRMSRISDLIGVDLHDPRARLKLAFALQVRKLVALPE"
     gene            complement(13066..>13293)
                     /locus_tag="PLW15_02870"
     CDS             complement(13066..>13293)
                     /locus_tag="PLW15_02870"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOT33374.1"
                     /translation="GKTVLVTAHGNSLRALVKHLDHISDDDIAELNIPTGIPLVYELD
                     DAFAPTGPGQYLDPEAAAAGAAAVAAQGAKK"
BASE COUNT         2261 a         4384 c         4429 g         2119 t
ORIGIN      
        1 cctccacaat tggagttctt ggtgttgttc ttctatccag ccaagtggcc agagcgcatt
       61 cttcgacacc tctagcctcg accgatgatc atcgagtcgg gcttcggcat ccacctctac
      121 ctcttccacg atgagatcga tcgcgagagt tgggaacttc ggatcgtgtg tctcgacgaa
      181 cgcctccagc ccgtgcactt cggccgactc gcggaccggt tcgaggggtc gcttgatcac
      241 catgcagagg gcatcaggca agccgtgcat caggaagacg tgcgctactt cgcagtcgga
      301 caccgagtcg ctgattgcaa tccgggccgg aaccttgctg agttctatcg cgcagagcgc
      361 gaggtagtcg cggaactcga gactgagggt ttcgtcttcc tggggcatca gacacacgac
      421 agtgatggat ggagctcaag cgggccgatg caccggttcg agagctacgt tgacggagac
      481 gagttcccca gagtgcttgt cattccgccg ccgcatccat tcgtcgactg cgagtgcgcg
      541 gtctgcggtc cacgaaatgc tcaatgggcc aaggcgcgcg tccagcactc gaaaccgctg
      601 tcctgatttt tgtattacga taatcgccca gggatacaca cttcggccct caggcacgga
      661 gtctcggccg aagacgcaat ccttgcggca actgaatacg tagtgaaggc cgaattggcc
      721 gaagatcgcg aacttcgact cggcttcgat tccgcgggca gactcctgga aacggtcgtg
      781 gtgattctga gcagtgggca acaggccgtg atccacgcga tgcctgcgcg acgaacgttc
      841 cttgcgttac tcgagaggaa gacatgacgc acgtcacaat caacggggtc cagcttgaag
      901 gtgtcaccgt aggaggcgtt ccggtcaccg acgcgatagt cgagaaatgg gcggacgagg
      961 ccgagcacgg atacgacatc gcgaccttga aatttcgcgg tcggccgcgg atgggcagtg
     1021 agatcgcgaa ggtcactaca ttgcgcctgg agccggccct cgacgttgca ctcagcgaac
     1081 gcgccgccaa ggacggtagc tcccggagcg aggtcattcg ggcggcacta cgggcttggt
     1141 tggagagcgc ctagggtctc gatcacgccg gggccgctcc cagccgggac gaatgcgtca
     1201 tccaacgaag actacggcag caccagcacg tgccgccgat cgccggccgc gagcatcttc
     1261 tcgatgcccc ggtcgaaggt cgcgagcagc accccggagg aggcggcgag gttcacgagg
     1321 tgggcatccg taacctggcg ggtcgtcgcc agggccgcga gcgatacggc gggctgggca
     1381 aggctcgatg cgtcgggcag gaactggtga ccgggaacag cacgcatcgc tgcgagcagg
     1441 gcgagtgcat cggccatcga gatccgctcg gtcacgacgg tcggattcgt cgagagccgt
     1501 acgaatccag actctgtcac cggggtcgtc atccagtcac cgcgacgtgc cgcgaaccag
     1561 gtgtgggcgc gccggtgatg cacgtgctgg ggccatgcca gtgcgacgag aacgttgagg
     1621 tcgagaaggt gcgattcagt cggcatcgcg ctgatcgttc accatatcga gcgtgatcgg
     1681 cgcagcatcg gcactcacgg tgaagaccgg aaacccgctc tggccggtgg tggacgagtc
     1741 acccgcggcc ttgaggccac gctcagcgag acgcgagacg gctgctccga gcgagatgcc
     1801 ttcatcgcgg gcgatcgcgc gcgcaacggc gatcactgtg tcgtcgagat caagcgtggt
     1861 gcgcatcacc gcatcataac atcacgatgt tactcggcta tcgacgcagt cacccgatcg
     1921 accgcgagcc cgctggacgc agccgcgagg cgagagtcat tcgtccacag ggcgtcgcat
     1981 ccgtgcagct gggccgccgc caggtgcaga gcgtcggccg ttcgcagggc gtgccgagcc
     2041 cgcagttcgg cggcccgcag aaactcgggc agcccgagcg acaacagttc gcaccgggca
     2101 atcgcgttgg cgtactggtc gcgcagggcc aggtcgtcac gcttgagtgg gccgaccatg
     2161 cactccagca ccaccagcgg tgagatcgcc actctgttgc gcgagctact caggtggcgc
     2221 ctgaccgtct ctccgcgccg gccctgatcc tctacggcgt agatgagcat gcagctatcg
     2281 aggtagatca gtcccatgct tcacgctcgc gcgcgatctg ctcgtccagc tcctcggcag
     2341 accgcacagc gcccgccggc agcggatgct cctggagcca ctgcacgagg gcctccccgg
     2401 tgttggctgc gtcatcgacg atcggaacga tgcgtacaac cggacggccg cgcttggcga
     2461 tgacgacatc ctcgccgttg gtcgcagccg tgatcagctg agagagccta ttccgggcgt
     2521 cgaggatatt gacgggctcc atccggttct ccccaagctg gcgtggttta tggctaggtt
     2581 gagtgtacat ccgcgtagtc tcacacgcac gaggatcacg gtctccgggt tctccacacg
     2641 tcgcccccga gggccgtgcc gacgacccgg tcccgcgcat cctgacttca tgactgacgt
     2701 gatgaaggcc cgatccgacg ccgagttcat cgggctgatt caggaactgg cgggttacac
     2761 cgcaacgaac agcctggtgt gcatcgccgt gcacgggttg cactattaca gcgactttcg
     2821 cctggacctc ccccaccgca agcgcaccgc tgactgccga gaactcgctc tggaggcgat
     2881 cggcatcctc agcggcatga gcaggatcga ccgtgtcgcg attgtcatct ataccgacga
     2941 gaccttcgcc gcacaccacg gcctgccgtg gctcgagttc tataccgcct ttgccgaacg
     3001 ggtgcacaag caggggttcc acttctccgg cttcttctgc atcgccgccg atgggtgggc
     3061 cgactacttc gatcgcgact acccgcggca ggggcgagcc ctgagcgaga tcccgagcgg
     3121 tccagtcgag ctgccgcagt gggcatccga atgagtatta ctacagccgg taagactacg
     3181 ctgggaccca tgcgcatcac gtcgaaggga caggtcacga tcccccagcg ccttcgcgag
     3241 cgctacggtc tcatgcccga gaccgaagtc gagttcgtcg aggtcggcgg cgaaatcgtg
     3301 gtgcgcaggg ccggggctca gagcgcggac cgggcccagc agatcgtgga ccggatgctc
     3361 ggggcaggcc attccccact gaccaccgag gaaatcattg cgatcgcccg tggctacgac
     3421 gactgaggtt cccgtcggtg agtcgttcct catcgactcg aatgttctcc tcgacatcct
     3481 cggcgcaggc ccgtgggccg catggtcaca ggatgtcgtc gccgcgtgcc tccgcgccgg
     3541 accggtgcac atcaaccccg tcgtctacgc cgagatctcg atgacattcg ccaacccgat
     3601 cgagctcgac gctctcgtgc ctcccagcgt attcaggcgc ttatcgatcc cctatccggc
     3661 cgcatttctc gctgcgaagg cgcatcaggc ctatcgccgc cgcggcggaa gtcgagcagc
     3721 aactctgccg gacttcttca tcggagcgca cgcgcttgtg gacggcctga ccgtggtcac
     3781 gcgggatccc cggcgctacc gagacgcgtt cccccaggtt cgggtcgtcg cgccgtaggt
     3841 ccagctgcac tcgatacacc gctgcgagag cagcaaggag agggcccgac cgagtggtcg
     3901 ggcccttcct ttgcggggcg ctggtctcga tacgcggctt cgccgctact cgaccggcgg
     3961 atttcctacg gctgcaagtg gtcgcgccag ttgccgctct cgatcaccgg atgcttcttg
     4021 gccatggcct cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtggtcgc
     4141 gccagttgcc gctctcgatc accggatgct tcttggccat ggcctcgtcg tagagcacgc
     4201 ccggcactat gcgaccgtcg gcgagctcga ccgggccgcg gtacaggcca gggggttcgt
     4261 tctcgatgac tcgaagcaac acgtcgatcg gcagcgtgta cagctcgccg gacacggcca
     4321 cgccatcctt ctccacctcg tacatgccgg ggtgaacgcc accgatgtcg tgcatccggt
     4381 acttcggagc cgtcttcatc tcggcgatga actcggcacc ctgcaggttt ccatgcagtc
     4441 cgaggccacg catgagcgtt ccgttgacga agagcaggtc atcctgaagc acgacctcct
     4501 tgtccttacg cgccgcccaa ccgaagccga gcagcaggat catgaggaag gcgtacccga
     4561 gcgagacacc cggtgaggcg ttgatcccga cctgcacagc gttgatcagg ccgaagaatg
     4621 acagcaccat acagatgccc gacgtgaccg ccgccgcgta catcctgcgg tcgatcacga
     4681 acacggctat ggagcccagc aacacgcccg ccaacaccgc gcccgatccg aacagcgtga
     4741 gcccctggta gatcacacca ttgcccgcca gcttgtcgag gcccacctcc gcagcattgg
     4801 tgccagctgc gccgagcgaa ttgtcgatca ggccggatgc ccactgcgcc aagctcggca
     4861 acatcgcaag gatgatcgcc ggggcgtagc gcagcgcgct cacgttggtc gcctgtgcgc
     4921 caatcaccag accgatgaac agcaggatcg gcaccagcgc ctgcatcggg aatatcgcga
     4981 ggaacaaacc gaccagaccg ctgaagcaga ccagcgcaac gacgattccg gtgaccagcg
     5041 agtagccgat gcggccaccg accttcttcc agccggggtg gccgatgtag acggccggcg
     5101 ggaacggcga gcccaggaac gacccgatga tcgcgcccag gccgtcggcg gtcagaaccg
     5161 cgcgagccga gtagcggtca ccggcggccg ccgccgattc gacgttggtc atggcctcgg
     5221 tgaagttgta gatgccgagc gggatggccg tcgccagcag cggggcgatg tcctgaaggc
     5281 ccgcgagaac ctcaccaccg ggaatcggga agtagattcc gatcgaggca atcgactcag
     5341 cgaccgcgct cggcttgagg atgtccgacc acccgagcag cacggcgatc cacgcgatca
     5401 cggtgcctgc aaccacggcg acgaggccga cgggagcgtt gaacggcatc ttccggaacc
     5461 cgagccagcc gacgaggatg aagccgaagg tcacgaatgc aatccacggc gcctcccaca
     5521 tctgggcggc cgggctcatc gagatgaacg tgatggagat acccgcgagg gcaccgagca
     5581 ttgccgctcg cggcgtccac ttgcgaatcc acggtccgaa gatcgcaccc agcaggatga
     5641 tgaacccgac aatgaacgcc cacgcaagtc ccgcgtgcca cgccttcagc gggtcacccg
     5701 actgcaggaa caccggcagc atgacgacga gcacgacgat gaacatgtgc ggcacgctcg
     5761 gcccgtaggg cagagccgcg acatccgttc ggttctcccg tctggcgagg cgcctggcaa
     5821 gaatcgcgta ccagatgttg cccaacggaa gcgcaatacc cagcgccggg aggatgcgac
     5881 cgaagacgac attgtcgggc atctggatga ccgccaggca gagcccggac agcacgatca
     5941 cgttgagcag gacgttggtg ccgagcccga acagcccgtt ccagtcgccc ttgacccacc
     6001 acttgatctg caccggtgca gaagtggtgg tcgcggtgga gttgctactc atttctttgc
     6061 tccaatctcg gtggttgccg aatcgactgc gtctaggaga ctcgtcgacg tgccgaccca
     6121 gccgacgatt ccgccctggg ccttgaacat gttgagcgcc gactcgtaga actcagcgaa
     6181 gtacgagccg gtgcagtcgg acaggatgag gcactcgaaa ccgcggtcgt tcgcctcgcg
     6241 ggcggtcgac tgaacgcaca cctcagtggt gacccccgtg atgatcagct gctcgatccc
     6301 cttggcgcgc aggatcgtct cgaggtcggt ggcgtagaac gcccccttgc cgggcttatc
     6361 gagcaccact tcgccctcaa tgggtgcgag ctccggaatg atctcgtgcc cggccgaccc
     6421 gcgagtcagc aatcgaccca tcggtccgtc gtcgccgatg cggagggtcg ggttgccgcg
     6481 atcccgcttg gccgggaaca ggtcggcgag gtcctcgcga tgaccctccc tggtgtggat
     6541 gaccatccac cccttctcgc ggaacatgtc gagcactgcg cgcgtcggct tgatcgagcc
     6601 gcgcagggtg gagacgtcgt tgccgaggga ctccccgaag ccacccgtct ccatgaagtc
     6661 gcgctggaag tcgatacaca gcagcgccac cttcttcggg tcgaacgtga acccgtacgg
     6721 ttctgccggc accgtgaccg tgccagtcgt ctcattcatg cgtgacctct ctactcagcc
     6781 gttcttcgtg acgatagttc ccgcggtgcc ggcaagagca tccggaagct tggagatcga
     6841 ggtgatgatg gcgcgcgttc cgcctgcctc gatgaagtcg atcgctgacg ccaccttcgg
     6901 gcccatcgac cccggaggaa actgcccggc agcgaggtgg tcgcggagct catcgacccc
     6961 cacagttcgc agcgcacgct gcccgggagc gttgaagtcg acatagacct cgtcgacccc
     7021 ggtgacgaga acaagagtgc cgacgccgat ctgtccggcg agcagtgcgg atgcccgatc
     7081 cttgtcgatg accgcatcga ccggatgcca ccccgccgag tcgcgcacca ccggaatgcc
     7141 tcccccgccg gccgcgatca cgacagcgcc gctgtcgacc agggtgcgga tctggctcgc
     7201 ctcgatgatc gtcttcggaa tcgggctcgc caccacgcgg cggtaggtgt tctcccccat
     7261 ctcggtcacc acccacccct cctcagcgac gcgcaaccgc gccacctcgg cggtgagctg
     7321 cccaccgatc ggcttggtcg gcttctcgaa tgcgggatcg tcggcgtcca cttcggcgtg
     7381 agtcagcacc gcgaccgccc ggatgccgct gtgccgcgca tcgagggcgg agtcgatcgc
     7441 gaccgcgagc atgtgaccga tgccaccctg cgagtcggcg accgcgagcc acaacggaag
     7501 ggcgggcagg ccctcgacca tggcatccgg tgccacgagc tcgccgcggc gcagaatgta
     7561 gccgacctgc gggccattgc cgtgggtgac gacggcggtc cagccgtcga ccaccagggc
     7621 ggcgaccgac tcagcgaagg tcgcggcgcg ctcgagctga cggtcgacag agccgggctc
     7681 gccgtcgagc acgagggcgt taccgccgat ggcgatcagt acggtgttgt cgttcgtggc
     7741 cgtcatgtgt gtgctctctg ctcggcgagg aatcgggctg ccttgaggaa gacctcgctc
     7801 ggcgcggtca cgatgcccgc gccgatctgg ccgacgcccg cctccttgtg cgcgatgccc
     7861 gtgttgatga gcggcagcac gccggtgtcg agaaccttga gcacgtcgat acccgacggg
     7921 gtgccagcga agttgagcgg cggcagaggg taggccgggt gcggaccgag cgtgatgcgg
     7981 cgcatcgagc gggtcgccgc gagcgcatcg ttcggggtac ccccgacgaa ctgggtgatc
     8041 gcgggggatg ccgccatcgc gaatccgccg aggccgagag tctcggtaat ggccgagtcc
     8101 ccgaggtcag ggttggcatc ttcgggtccg aagccgggga agaacaggcc atccgcgggg
     8161 ccgacggggg tggtgaacca ctcggttccg ctcccggcga cccgaaggcc aaactccacc
     8221 ccgttgcgcg ccatcgcggt gacgagagtg gagttctcga cgccgatcgc ggcatccaga
     8281 gccagcttgg cggcggccat cgagatgttg agagcgaagt gatcgttgcc cgccaggaag
     8341 gcgagcaccg cgacgctgtc ggaattgggt tccagctcgg cgatcgtcgg cgcgagtcgg
     8401 cgggccagga gcgaggtcgc tgcgacgttg cggttgtgac cctcgtcgcc catcgacagt
     8461 gcctgaccga tgagcgaggt gacgtcgatc ggaccgtact tctcgagcgt gcggtcgagc
     8521 accgggccga gcacatcgcg catccaggtc agccgagtga tgacttcgga gtcgtacgca
     8581 ccgaaccgca gcaccttgcc gaggccctcg ttgaggctcg caaacgcctg cctgccgtcg
     8641 tcgaggtcgg tcgccaccag caccggcatg gatggcgcga tgactcccgc catcgggcca
     8701 acggccccgt ggtggtgaca gggtgcgaca gcgatctcgc cggagtcgag catcctgagc
     8761 gcatcgtcgg cgttgtcggc ccagccctcg aacagtgtgg cgccgatgag tgcgcctcgc
     8821 atcgggccgc acatgcgctc aacctcgatg ggcggaccgg cgtgcagcag cgtgcggccc
     8881 tcggcgatgc cgggcatcac ctcacgggcc ggtcggatgc gcgtcaactg cggcttcacg
     8941 gcgtgcacct tcgcgatggc gacggcgttc gcggcatccg tcacctcgtt gtcgagggtc
     9001 agcgcgaggc gagccacatc ggcgtcgccg aatgccgggg gacgccagtc gagctgcgtc
     9061 acctgcgccg cgggaacgcc ctccacgatc aacggaaggc cagcgttgat gacgctgagt
     9121 tcgtcggtaa ggatgctcat gcaacacctc ccgcggccag tcgggccgcc gccgcattgg
     9181 tctcggcgag cacgacgccc gcactggtca ggatgccgcg ctgccgcgcc agcccctgcg
     9241 gatcacccgc cgtaccgcac accgagccaa caaccagcag cgttctgccg tccgccgcgg
     9301 ccgcggcttt cgcctgagcc accgccgtcg ccacaggggt tgcggggtcg gtgttcgccc
     9361 cgtagccgag aacgaggtcg agcaggatcg tgccgacggt ggcatccgag cccgccgccg
     9421 cgatcttctc ggcacgcgac tcggggtcga tcatcgggtg cggcttgccc gccgtgtact
     9481 cgtcgtcgcc gaggtcgatg atctcggcga cgactccggc gcgcttgagg atgatcttcg
     9541 actcggacgc gagcgtgccg cccgtgtaga ggccgagcac gcgaccgggc ggcgcgtgca
     9601 ccggctcggg ctcggtgacc gtgaacgggt gcccagcgag cccggccgca gcgagcgcgc
     9661 ccccctcgag agtgccgcgc accaggacgc cgtcggtgtc ggagtcgtct tggccgagga
     9721 agcaggcgac gaccggggtt ccattggcgg cgatgccggc gagccgggcc agcagcgcgt
     9781 cggcgacctc gggcgccggg ggcttcgaga ccatcatgat gactgtcgtg tcggggtcgt
     9841 cgatcaggaa gtcgagagcg aggttcgcca tcaggccgcc cacctcggcc gacaggtcgc
     9901 gaccgccgac accgatcagc tgcgacactc cggcgcccgc gccgtcgagc agccgcgaca
     9961 cctcctgtgc gccggttccc gaggcggcga cgatgccgac cgaacctcgc gccaccgcgt
    10021 tcgcgaaccc gagcggcacg ccgtcgagga tcgcggtgcc gcagtcgggg cccatcatga
    10081 gcagtcgctt ctcgactgcg agggtcttga gccgcacctc gtcggccacg gcaacgttgt
    10141 cggagaagca gagaacgtgc attcccccgc gcagcgcctg ctcggcaacg acgggtgcgt
    10201 aggttccggc gatcgagatt gcgaccaggt tcgcgggatg ctccgccagg gccgcgacgc
    10261 tgacgccctc gtcgatcgtc cccggtcgtt gctcctcggt tgccgacccg gcgtccacag
    10321 ctgtcagccc cgcctcggct gcgtcgagtg ccgccgtcac gatctccgag tcggtgccgc
    10381 gcaccacgaa gacaagatcg tcgggggccg cggtgagccc gtcgggaagc atgcccgagt
    10441 cggccaggat gccgaggttc gcgggagtag ccatgacagc accgacccgc acgaccccgt
    10501 cgcgcttctc cgtcgtcgat gcgatcgcca tcagagcgac cgagtcgcgg tagaagtgcg
    10561 ggagcactcg ggaggagaga accgtgtcgc tcatgcggca cccttcgttc gatgcgcgag
    10621 ctccgctcgc agtgtggcgg ccaagccggt gagaaggtcg accccggatg tcgcgcccca
    10681 ccggctcgca cacgcgagga tcgtgcgggc gggcatcccg tcgaccaggc cgtcgacgag
    10741 ctggtggacg tgctccgcga agcgaccgga tgccgcggca acgatgtagt ggcgggaggc
    10801 cgccgtcgtc gcgccgagca gtggggtcac cgcagcggac agtcggctcg cggcatccgc
    10861 taggccaagt gcagcgaggg cggcaagcgt gccgaccacc atgtcgtcac ctgacggggt
    10921 ggtgccgggg ccggcaccga tgagctccgg cagcagcacg cgcgggtcgc cgtggatggc
    10981 cgcctcagcg agtgccgggg cgagcgaacg cacccgggcg aggccgccgt cgaaggatgc
    11041 cgcaggggaa gcgttgcccc gtgcgagctc ggcactgacc gcatggatcg cgccggcatc
    11101 gaggcttcgg ggggtgatgt gcaggtcgac cggcacgaga gttgcggtcc gcgaccaagc
    11161 ggagaggtcc acgggatgcc cgggcgcgac ggcggagagc tcgcgacgaa cacgggcgaa
    11221 ctcggcccca ttcggaatgc acacaccccc gggggtgacg ccgccactcg cgttggcaat
    11281 cgtcagcagg cgctgggttg tggtggatcg gagtacgacg accgattgtc cgagatggac
    11341 gatcagttgg tatccagctg agaacccgtc ggcggcgctg gcaccgagcg tcgcatcgat
    11401 gcgagcgaga tcgggttccg caatcattgc cgccatcgcc atacttggga atagtagggt
    11461 caacgatcac ggtatggctc ggtccggaaa ggacgacaat ccgggcggcg attgtccaga
    11521 agggacaaag gagttgatgt gagcacgatc acagtgcgtg aggttctcac tctgcccgtg
    11581 ctccgcggca cgttcgtcgt cgccgggcaa cgcggtctga gccgcgccgt cactggggtg
    11641 aacgtgatgg aggttcccga catcgagtcg ttcgtcaagg gcggcgagct gctgctcacg
    11701 acggcgtacc cgctgcgcga gcatcccgaa gatctgagca ccctcgtgcg cacactctct
    11761 cgcctcgggc tggccggtct cgccgtcaag accgggcgct acctcgagaa gcttcccgac
    11821 gaagccttgg cggtggcgaa cgagctcgac tttccggtga tgctggtcgc cgagcacacc
    11881 tcgttcaacg aggtcatcgg cgccgtgctc gccgtcgtgc tgaccgagta cggggccgag
    11941 ccggcgggcg cggaagcgat ccgggagcgt ctcaccggtg tcgcactcgc cggcggtggg
    12001 ctcgtcgaga ttgcccgaac ccttgccggg gcgctgggac ggcacgtcgg catcctcgat
    12061 ccagacggcg cattgctcgg cgaggggggc ggcggcgaac gcgccggcaa cgacgcggca
    12121 cacgagccat ggtcattccc gatcaccgtg ggtggcaccg aacgggggcg catcgtcgtg
    12181 cgcggcgacg acgaaccgac gctgggccag cggcgactca tccggcaggc ctgcttcgca
    12241 gccgggatgc acatcgcgca ggccgtcgcg agcctcgaac tcgaccgcca gatgcgggtg
    12301 ctctttctcg aggagctgct cgcgggcaag accattgacg agtcgcttgc tcgcgagcgg
    12361 tcacggcttt tcggctggga cttcggctcc gatcacgtcg tcgtgctcgc ccggctgagt
    12421 cacgagcttg cggatgccac ggtcgcccgc gccgccgaga ccgggttcat caggggagca
    12481 gtcgcctggg cgcgcgggca ggaggcggtc gcgatcgtgc cggcggcgag catcccaggg
    12541 gatggaacgc cgtgggtcga ggcggcgcac tggggtgaga cgctcgcgcg gctcggcggc
    12601 gggaaggtca cggttgctac gggaagcatc gcggaatcac cgtcgcggct cgcggagtcc
    12661 cacagcgccg cccggtcgac gttggctatc gcccaggcga ccggacgcgc gaacgcccgc
    12721 cacgacctgc tcgcgctgga gcgcgtgatt ctctcggtgc ccgatgacct gctcgccgag
    12781 cttgtcgctg ccgagctcgg cccgctgatc gcggtcgacg ccgcgaccgg ctcggagctg
    12841 tgcgagacgc tcgagatgtt cctcggcacg ggcaacgccg ccgatgccgc tcgccgactg
    12901 ttcatccact acaacacgat gaagcaccgg atgtcgcgca tctccgacct catcggtgtc
    12961 gacctgcacg acccacgcgc ccgcctcaag ctcgccttcg cgctgcaggt gcgcaagttg
    13021 gtggcactgc cggagtgatc tcggcacacc cagtcgcgcc gaccgttact tcttcgcgcc
    13081 ctgcgccgcc accgccgcgg caccggcggc tgcggcctcc gggtcgaggt actggccggg
    13141 gccggtgggg gcgaacgcgt catccagctc gtagacaagg gggatgcccg tgggaatgtt
    13201 gagctccgcg atgtcgtcgt cgctgatgtg gtcgaggtgc ttgacgaggg cgcgcaggga
    13261 gttgccgtgc gcggtcacga gcacggtctt gcc
//
LOCUS       DAOSOU010000030        13279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4697_length_13279_cov_6.556866, whole genome shotgun sequence.
ACCESSION   DAOSOU010000030 DAOSOU010000000
VERSION     DAOSOU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 13279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13279
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4697_length_13279_cov_6.556866"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLW15_02875"
     CDS             <1..234
                     /locus_tag="PLW15_02875"
                     /inference="COORDINATES: protein motif:HMM:NF020122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HOT33375.1"
                     /translation="QVMKSVYADAGITAADLPAGLEVVRRRGETHDYVTVINHGDTVA
                     SVPVSGFDLVDQSEVTRGTPLAAGAVAIVRTTT"
     gene            complement(231..554)
                     /locus_tag="PLW15_02880"
     CDS             complement(231..554)
                     /locus_tag="PLW15_02880"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HOT33376.1"
                     /translation="MTIFHIAHAVDWHDAERTGEYRVSTRGRTLDEVGFIHASSEAQV
                     VGVANAFYSDDPLTLIVLELDEARIEAAGIRVLWEDVAGELFPHVYGPIRPEFVVSVT
                     PLALG"
     gene            578..916
                     /locus_tag="PLW15_02885"
     CDS             578..916
                     /locus_tag="PLW15_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOT33377.1"
                     /translation="MNFDEAMDACAGHPATELGYPFGEGVHVFKVVSKVFAILGDEAG
                     RARITLKADPEDARALVATFPEVTPGYHMNKKHWVSIDLPSAGAPLDELIRDSYELVV
                     ATLPRDKRPK"
     gene            complement(990..1679)
                     /locus_tag="PLW15_02890"
     CDS             complement(990..1679)
                     /locus_tag="PLW15_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT33378.1"
                     /translation="MSLATIDRAPISTPAPVAAPAVPRRLHAVPQGTEARGFALYVGL
                     DEFSAIEAGTDLSYVVDQLKKRLAELVPSAETYASVALAPVGSGGRDVDVVRLALQDP
                     AALAKHRQVEEPAPKPRGGVVIDISRKRVQLDGETAPLTYKEFELLQYLVLREGRTVG
                     RDELIASLWTAGDDEIPNERTIDVHVRRLRAKLGDYEDIVRTVRGVGYRFDRHADVSI
                     RQASTPSPDFF"
     assembly_gap    1910..2009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2219..2551
                     /locus_tag="PLW15_02895"
     CDS             2219..2551
                     /locus_tag="PLW15_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT33379.1"
                     /translation="MVAVSRRVRAARRRQRRMKAADNDLTAAEWADILTAWGGCAYCG
                     GTGMPLQRDCVQPLSRGGRYTVSNVVPACRTCNASKSNDEVTSWLRRTKRDERAFLQR
                     YAEIAART"
     gene            complement(2644..3276)
                     /gene="upp"
                     /locus_tag="PLW15_02900"
     CDS             complement(2644..3276)
                     /gene="upp"
                     /locus_tag="PLW15_02900"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOT33380.1"
                     /translation="MRVHVADHPLITHKLTVLRDKTTPSPTFRALAEELVTLLAYEAT
                     RNVRTHAVTITTPVAETQGLAISDPKPLVVPILRAGLGMLEGMVKLVPTAEVGFLGMV
                     RNEETLQPDIYAERLPDDLSNRQCFVLDPMLATGGSLIAAIEYLFARGAVDVTAVCLL
                     GAPEGLAAVEKATEGRDVTIVLGALDEKLNEVGYIVPGLGDAGDRLYGTV"
     gene            3306..3764
                     /gene="tadA"
                     /locus_tag="PLW15_02905"
     CDS             3306..3764
                     /gene="tadA"
                     /locus_tag="PLW15_02905"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOT33381.1"
                     /translation="MSTTDEWMRAALVEARLALASGDVPVAALVLDAAGIPIGVGHNE
                     RELTGDPTAHAEIVAIRAAAKATGSWHLENTTLVVTLEPCTMCAGAILQARIPRVVFG
                     AWDEKAGAVGSVHDVLRDRRLPHRVEVIAGIREAECATLLRDFFADKRVE"
     gene            3846..4388
                     /locus_tag="PLW15_02910"
     CDS             3846..4388
                     /locus_tag="PLW15_02910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HOT33382.1"
                     /translation="MTRALVLIDLQLDYFAGGAHPLVGPDAAVANAAVVLEQFRASGE
                     PVIHVFEIWDEPEATFFRPGTPGVEIHPAVAPRAGERVVQKSNANAFLDTSLEADLRA
                     LGADEVVVVGMMTCVCIDSSVRAAADLGFDVTVVGDACAAPDLEFDGTIVPAAQVHAA
                     FLAALGENFATVVTAEELLR"
     gene            complement(4404..4757)
                     /locus_tag="PLW15_02915"
     CDS             complement(4404..4757)
                     /locus_tag="PLW15_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33383.1"
                     /translation="MEILKLVLVAAHFLGMAAIVGSFFVQMRRKSDFVLTPLLAGAIT
                     QVVTGLALVGLAEATGDDLIYAKIAVKLTIAVVVLVAAILAVVTQRKGGRVQPWFHAA
                     GGLAVINVLVAVFWG"
     gene            complement(4779..5741)
                     /locus_tag="PLW15_02920"
     CDS             complement(4779..5741)
                     /locus_tag="PLW15_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT33384.1"
                     /translation="MSTTGGTRAIIAALLANLGIAITKFIAFLFSGSSSMLAESVHSL
                     ADTGNQGLLLLGGRKSRKRADAAHPFGYGRERYVYAFVVSIILFSVGGVFSIYEGIDK
                     LQHPHELEVPWLPILVLSVAIVLESFSLRTAIKESNHVRGKQGWVSFIRHAKAPELPV
                     VLLEDVAALTGLVFAMFGVGLTILTGNSAWDAIGTLAIGVLLIVVAVVLGIETKSLLV
                     GEGAGSRDADKIREVINANTDVEALIHIKTLYLGPDELLVAAKVAFAAKSRLSDVATS
                     INTLEAAIRAAVPVARVIYIEPDVYQAPGASNPTTDSIVIKGAD"
     gene            5765..6592
                     /gene="proC"
                     /locus_tag="PLW15_02925"
     CDS             5765..6592
                     /gene="proC"
                     /locus_tag="PLW15_02925"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT33385.1"
                     /translation="MSTLGSIAIIGAGAMGGAILGGLLAPGVTVDGDIRVTNRTEAKA
                     ALVRRPGVDSVALETAPDGNALAVRGASVVIIGVKPAMVPETLAALAGDLAPDALVIS
                     VAAGVTTATMEAIVSQPVLRAMPNTPSLIGKGVTGLAAGSRATAEQVALGRAVFETVG
                     TVVEVPESQIDALSTISGSGPAYVFYLVEQLTRTAVNLGFTPAQAAELVTGTFLGSVE
                     LLVASGREPEELRRQVTSPNGTTQRAIAELEKADLTGLFDRATASALARAKEMAEGR"
     gene            6685..7029
                     /locus_tag="PLW15_02930"
     CDS             6685..7029
                     /locus_tag="PLW15_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33386.1"
                     /translation="MATKKEESAPAASASTPSEPVLAQSAPAQPLVAPRRGLGGGAIA
                     GIVVGSLVVAGALFGGGVLVGSHLPSGMTQSQFGPGFGPGDGDNDGRPPFPGGGPGPV
                     QPGQNDGDTDSN"
     gene            complement(7052..7723)
                     /locus_tag="PLW15_02935"
     CDS             complement(7052..7723)
                     /locus_tag="PLW15_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT33387.1"
                     /translation="MVEKIAHDAPVLVIGLGRFGAATAGQLQRLDREVLAIDEDAGLV
                     QKWSERVTHAVQADARSIDALRQIGAQDFSIAVVAVGSSIESSVLITANLVDLKIPQI
                     WAKAISKSHGTILTRIGANHVIYPEAEAGERVAHLVSGRMLDFIEFDDDFAIVKMYPP
                     KPIRGIPLSQSLVRKKFGVTVVGVKSPGADFTYATPETIVSNHDLIIVSGNSTDIEKF
                     AALSS"
     gene            complement(7716..9134)
                     /locus_tag="PLW15_02940"
     CDS             complement(7716..9134)
                     /locus_tag="PLW15_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT33388.1"
                     /translation="MRGRPVSRRTVFGRLQDVVDAFVASSPARFAIVIFASLILVFTA
                     LFSLPIATSSGKQAPLVDALFTAVSVICVTGLSTVDMATYWSPFGHVLVFLGVEIGGV
                     GVLTVASILGLVISRRLGLRQKLLAAGDANPLRIRKGPIAEGQAIRLGETGNLLRTVA
                     ISALVIEAGVAILLFPRMLIQGVPVWDAAWQSVYISAMAFTNTGFLPTSTGLAPYAGD
                     VWFLSVIMLGVVAGAIGFPVIYALSRNLRNPRRWSLHVKLTLITFALLLVMGALAYFV
                     LEFDHAFRGLDGGQRVLQSFFLSSMARSGGFSTIDISQLDGSSLLVTDMLMFIGGGSA
                     STAGGIKVTTLAILFLAAFAEARGRDDMEAYERRIPQDVLRLGVSVVLWGATTVAVSC
                     IVIMMMTDAALDHVLFDVISAFATCGLSTGFTANTTDAAQYVLAATMFFGRIGTVTLA
                     AALAASQHPKLFRQPEERPIVG"
     gene            9185..9664
                     /locus_tag="PLW15_02945"
     CDS             9185..9664
                     /locus_tag="PLW15_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT33389.1"
                     /translation="MADIFDVIADPTRRDLLQVLLERYVAADSETGEISVGDLVERLG
                     LSQPTVSKHLRVLRDHGLVSVREDGQHRYYRLEATPLEELEDWLQPYLSADFDDTFEP
                     ADGGPNAFAAWSGADVGETIGRRVAEGTHQARVVIQDAAEQVTKRLPKSFRRRPHRP"
     gene            9827..10015
                     /locus_tag="PLW15_02950"
     CDS             9827..10015
                     /locus_tag="PLW15_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT33390.1"
                     /translation="MSLSDVRFLTVAEVAEMMRVSNMTVYRLVHSGDLPAVRFGRSFR
                     IPESAVVAAIQTPIADVG"
     gene            10111..10209
                     /locus_tag="PLW15_02955"
     CDS             10111..10209
                     /locus_tag="PLW15_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOT33391.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLRKTRHQRRNKK"
     gene            complement(10305..11054)
                     /locus_tag="PLW15_02960"
     CDS             complement(10305..11054)
                     /locus_tag="PLW15_02960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT33392.1"
                     /translation="MSDAARRTPTPGPIVAFFDVDNTLMRGASVFYVGREAWRRRLIS
                     FRDIAVFAWHQFRFLAVGENRQHLAHAKERALGLVGGHTEEALVMLANDIYEKDILPN
                     LWPETVALTREHLAQGHEVWLITATPEIVAQVIAERLGLTGALGTKVQALEGVFTGEL
                     DGPVLHGDEKATVARLFAEQKRADLAQCWAYSDSSNDIPLLTMVGNRVVVNPDAKLLA
                     HANTHGWRVLPLKRSSITDARKQVKRDRKAG"
     gene            11067..11339
                     /locus_tag="PLW15_02965"
     CDS             11067..11339
                     /locus_tag="PLW15_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOT33393.1"
                     /translation="MVVPDVRLTLLSKPGCHLCDAARDAVTEVIAQLGAAASAVTVEE
                     LSILDDPELNERYWDEIPVVLVNGRVHTIWRVDPARLTRALEEAIQ"
     gene            11336..11635
                     /locus_tag="PLW15_02970"
     CDS             11336..11635
                     /locus_tag="PLW15_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOT33394.1"
                     /translation="MIRHVVMWKLSATDAPAKAAAIAAIAGALEPLVGVVPGLQSLMV
                     RPNVAYLETNYDAILVSDFDSVEALEQYQVHPAHVEAAAVPRSHAASRVTVDFEV"
     gene            complement(11632..11931)
                     /locus_tag="PLW15_02975"
     CDS             complement(11632..11931)
                     /locus_tag="PLW15_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33395.1"
                     /translation="MSKKIDAALKDLKKALNKHAEAVGGRTVSLKKSQRASARVAAAA
                     TAYAEAVHAKSGLGNPFADVLEPGLEATTLASLSAERDKLAKSLTGPIETQKAAG"
     gene            complement(11974..12153)
                     /locus_tag="PLW15_02980"
     CDS             complement(11974..12153)
                     /locus_tag="PLW15_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOT33396.1"
                     /translation="MAGKFVISKDKKGEFRFVLKAGNGEIIAMSEGYASKSGAQNGIE
                     SVRKNAPDAAVVDES"
     gene            complement(12202..>13279)
                     /gene="aspS"
                     /locus_tag="PLW15_02985"
     CDS             complement(12202..>13279)
                     /gene="aspS"
                     /locus_tag="PLW15_02985"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOT33397.1"
                     /translation="HDERVKLGGVEIKLEALDIAAAANPETPIADDTGVDKRMDWRFL
                     DLRQPKNNLIFRIQTTLEHAWRTYWVEHDFIELHTPKLMASASESKAELFEVEYFETK
                     AYLSQSPQFFKQMAQPAGFGKIFEVGPAFRADPSFTSRHATEFTSVDSEISWIDSHED
                     VMKLHEELLVAGFAAVKAKHGDEIEALFGVEVTVPTVPFPRIPLAEAKRIVAERGYEV
                     PRHDDDMDPEGERQIAAYVKETFDHEFVFLTDYASSIRPFYHMRHADDASLTNSYDLI
                     FNGVEISTGAQREHRIEILEAQARDKGLDPEELGFYLDFFRYGVPPHGGFGMGLSRVL
                     MLMLHLPNLRETTYLFRGPTRLLP"
BASE COUNT         2207 a         4266 c         4522 g         2184 t
ORIGIN      
        1 caggtgatga agtcggtcta cgccgacgcg ggaatcaccg cagccgacct gccggccgga
       61 ctggaggtgg tgcgcaggcg cggcgagacc cacgattacg tgaccgtgat caaccatggc
      121 gacaccgtcg catccgtgcc cgtctcgggg ttcgacctcg tcgaccagag cgaggtgacc
      181 cggggtacgc cccttgccgc cggtgctgtc gcgatcgttc gtacgaccac ctagccgagc
      241 gcgagcgggg tcaccgacac gacgaattcc ggtcggatgg gcccgtagac gtgggggaac
      301 agctcgccgg ccacatcctc ccagagcacc cggatgcccg ccgcctcgat tcgcgcctcg
      361 tcgagttcga gcacgatgag cgtgagcgga tcatccgagt agaacgcatt tgccaccccc
      421 accacctgcg cttcggatga cgcgtggatg aagcccacct cgtcgagcgt gcgcccccgg
      481 gtcgagacgc ggtactcgcc ggtgcgttcg gcatcgtgcc agtcgacggc gtgcgcgatg
      541 tggaagatcg tcatgggttg atcgtaggct gcgtgccatg aacttcgacg aggcgatgga
      601 cgcctgcgcc gggcatccgg cgaccgaact cggctacccg ttcggcgagg gtgtgcacgt
      661 cttcaaggtc gtgagcaagg tcttcgcgat cctgggtgac gaggccgggc gagcgcgcat
      721 cacgctcaag gccgatcccg aggatgcccg tgcgctcgtc gccacgttcc ccgaggtaac
      781 ccccggctac cacatgaaca agaagcactg ggtgagcatc gacctgccgt cggccggagc
      841 gcccctcgac gagctcatcc gcgactcgta cgagctcgtc gtcgcgaccc tcccgcgcga
      901 caagcgcccg aagtaaggtc gcgcaggaaa cgtcggaagc gcggctcaat cgcaccgtgc
      961 gccgcagggg cggcggcgca ccaagtaact cagaagaagt cggggctggg cgtggaggcc
     1021 tggcggattg acacgtcggc gtgacggtcg aagcggtagc cgacgccgcg aaccgtgcgc
     1081 acgatgtcct cgtagtcgcc gagcttggcg cgcaggcggc gcacgtgcac gtcgatggtg
     1141 cgctcgttcg ggatctcgtc gtcgccggcg gtccacaatg atgcgatgag ttcgtcacga
     1201 ccaaccgtgc gcccctcgcg cagcaccagg tactgcagca gctcgaactc cttgtaggtc
     1261 agcggcgcgg tctcgccatc cagctgaacg cgcttgcggg agatgtcgat gacgacgccg
     1321 ccgcgcggct tgggggcggg ctcctcgacc tggcggtgct tggcgagagc ggccgggtcc
     1381 tgcagggcga gacgtacgac atccacgtca cggccacccg agccgacggg ggcgagagcg
     1441 accgacgcgt aggtctcggc gctcggcacg agttcggcca ggcgcttctt gagctgatcg
     1501 acgacgtagc tgagatcggt gccggcttcg atggcactga actcgtcgag gccgacgtat
     1561 agggcgaagc cgcgcgcctc ggtgccctgg ggcacggcgt gcagccggcg cggcactgcg
     1621 ggagcagcga ccggggccgg agtggaaatg ggtgcgcgat cgatggttgc gagagacatg
     1681 tgtcctgtgt tccttgactg gggtgaaggg cctctgatga tgagggtgtg atccttcggg
     1741 gtactgcggg tggtgcgtgc ggatcaggcc gttatgaggg cggtgatccg ggagtcacgg
     1801 tcgagggtga ctcggcggga gaagccgatt aggcacacat tcgacaacac atggacgcgc
     1861 gagaacccat cacctcaagg gtggtcaggg agtgcgcgcg aacagtcatn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cgcgcgagaa cccatcacct caagggtggt
     2041 cagggagtgc gcgcgaacag tcatacatgc aactatcggg caattgcccg cggaatgcaa
     2101 ctgcggtaat cgagcgacgg aacggtaacg ctgtctagtt acctaaccgt tatttttctg
     2161 ggagttcgct tcgagccgtt ccgcgggcct tccggggccc gaggatagcc tgtgatccat
     2221 ggtcgccgtg agtcgtcgag tgcgagccgc tcgccggcgt cagcgccgca tgaaggccgc
     2281 cgacaacgac ctgaccgccg ccgagtgggc ggacatcctg accgcctggg gcgggtgtgc
     2341 ctactgtgga gggaccggga tgccgttgca gcgcgactgc gtgcagccgc tctcgcgcgg
     2401 cggacgctac acggtctcga acgtcgttcc cgcgtgccgc acctgcaacg cgagcaagag
     2461 caacgacgag gtcacctcgt ggctgcgccg caccaagcgc gacgagcgcg ccttcctgca
     2521 gcgctacgcg gagatcgccg ctcgcacata ggcgacgatc gcgtgggcgt gcccgtggcc
     2581 gaggccgtac tcgctcttgc cgtcgcgcag gggagatctc gccgggcgcg acccgcccga
     2641 acgctacacg gtgccgtaga gccgatcgcc ggcgtcaccg aggccgggga cgatgtagcc
     2701 gacctcgttg agcttctcgt cgagagcgcc gagcacgatc gtgacgtcgc gaccttcggt
     2761 ggccttctcg acggcagcca gaccttcggg ggctccgagc aggcagacgg ccgtgacatc
     2821 cacggcgccg cgggcgaaca ggtactcgat cgcggcgatc agcgacccgc cggtcgccag
     2881 catcgggtcg agcacgaagc actgccggtt ggagaggtcg tcgggcaggc gctcggcgta
     2941 gatgtcaggc tggagggtct cctcattgcg caccattccg aggaagccga cctcggcggt
     3001 gggcacgagc ttcaccatgc cctcgagcat cccgagtccc gcacgcagga tcggcacgac
     3061 gagcggcttc gggtcgctga tcgcgagccc ctgcgtctcg gccacgggtg tcgtgatggt
     3121 cacggcgtgg gtgcgcacgt tgcgcgtcgc ctcgtaggcg agcagggtca caagctcctc
     3181 cgcgagtgcg cggaacgtgg gcgacggggt ggtcttgtcg cgcagcacgg tgagcttgtg
     3241 ggtgatgagc gggtgatcgg caacgtgtac tcgcataggc tcaatctact gtggacgcct
     3301 tgccgatgag cacgacagac gagtggatgc gcgccgccct cgtcgaggcc cggctcgcgc
     3361 tggccagtgg tgacgttccc gtcgcggcgc tcgttctcga cgcggcgggc atcccgatcg
     3421 gagtcggaca caacgaacgc gagctcaccg gtgaccccac cgcccacgcg gagatcgtcg
     3481 cgatccgcgc ggcggcgaag gcgaccggct catggcacct ggagaacacg acgctggtcg
     3541 tgacgctcga accgtgcacg atgtgcgcgg gggcgattct tcaggcgcgc attccccggg
     3601 tcgtgttcgg cgcgtgggac gagaaggccg gcgcggtcgg cagcgttcac gacgtgctgc
     3661 gcgatcggcg cctgccgcac cgggtcgagg tcatcgcggg aattcgcgag gcggagtgtg
     3721 cgaccctgct gcgcgacttc ttcgccgaca agcgggttga gtagcgcgaa gcgcgtatcg
     3781 aaaccccgct gatcgagctt gtcgagatcc cgcccagcga gtccgtcgag atctaggctg
     3841 accccatgac ccgcgccctc gtgctcatcg atctccagct cgactacttc gccggcggcg
     3901 cccatccgct cgtcggcccg gatgccgcgg tcgccaatgc cgcggtcgtg ctggagcagt
     3961 tccgcgcgag tggcgagccg gtcatccacg tcttcgagat ctgggacgag ccggaggcga
     4021 ccttcttccg cccgggcacg cccggggtcg agatccaccc cgccgtcgcg ccgcgggccg
     4081 gtgagcgcgt cgtgcagaag agcaacgcca atgccttcct cgacacgagc ctcgaggctg
     4141 acctgcgtgc gctcggcgcc gacgaggtcg tcgtcgtggg catgatgacc tgcgtctgca
     4201 tcgactcgtc ggtgcgggcg gcggccgacc tcggcttcga cgtcacggtt gtgggggatg
     4261 cctgtgcggc gcccgatctc gagttcgacg gaacgatcgt tcccgcagcc caggttcacg
     4321 ccgccttcct cgcggcgctg ggcgagaact tcgcgacggt ggtcaccgcc gaggagctgc
     4381 tccggtgatc gagcctgtcg agatcagccc cagaacaccg ccacgagcac gttgatcacc
     4441 gcgagcccgc ccgcggcgtg gaaccagggc tgcacacgtc cgcccttgcg ctgggtgacg
     4501 acagcgagga tagcggcgac gagcacaacc acggcaatgg tcagcttcac ggcgatcttc
     4561 gcgtagatga ggtcgtcgcc ggtcgcttcg gcgagtccga cgagggcgag cccagtcacg
     4621 acctgagtga tcgcgccggc cagcagcggc gtcagtacga aatcgctttt gcgccgcatc
     4681 tgcacgaaga aggagccgac gatggccgcc atcccgagaa aatgcgctgc aacgagaacg
     4741 agcttgagga tctccatggg tcagtctcct tatcgcgcct agtcggcgcc cttgatgacg
     4801 attgaatcgg tggtcgggtt ggatgctccg ggcgcctggt acacgtcagg ctcgatgtag
     4861 atcactcgcg cgacgggcac tgcggcccgg atggcggctt cgagcgtgtt gatgctggtc
     4921 gcgacatccg agagtcggga cttggccgcg aacgcgacct tggcggcgac gagcagttcg
     4981 tcgggcccga ggtagagcgt cttgatgtgg atgagcgcct cgacgtcggt gttggcgttg
     5041 atcacctcgc ggatcttgtc cgcgtcgcgc gagcccgctc cttcgccgac cagcaggctc
     5101 ttggtctcga ttccgagaac cacggccacc acgatgagca gtacaccgat ggcgagggtg
     5161 ccgatcgcat cccaggcgct gttaccggtg aggatcgtga gccccacgcc gaacatggcg
     5221 aagaccagac cggtgagcgc ggcgacgtcc tcgagcagca cgacgggaag ttcgggcgcc
     5281 ttggcgtgac ggatgaacga cacccagccc tgcttgccgc gaacgtggtt cgactccttg
     5341 atcgcggtgc gcagcgagaa cgactcgagc acgatcgcga ccgacagcac gaggatcggc
     5401 agccagggca cctcgagctc gtgcgggtgc tgcagcttgt cgatgccctc gtagatcgag
     5461 aacacgccac cgaccgagaa caggatgatc gacacgacga aggcgtacac gtagcgctca
     5521 cgtccgtagc cgaacggatg cgccgcatcg gcccgcttgc gagacttgcg accgccgagc
     5581 agcagcaggc cctggttgcc ggtatcggca agcgagtgga cgctctccgc gagcatcgaa
     5641 ctcgaaccgg agaacaggaa cgcgatgaac ttggtgatgg cgatccccag attggccagt
     5701 agtgccgcaa tgatcgccct ggttccaccc gttgtgctca tgcgcccaat cctaggatga
     5761 acgcatgagc acactcggtt cgatagcgat catcggcgca ggggctatgg gcggggcgat
     5821 cctcggcggg ctactcgccc cgggagtcac tgtggacggc gatatccgcg tgaccaaccg
     5881 cacggaggcg aaggccgcgc tcgtgcggcg cccgggtgtg gattccgtcg ccctcgagac
     5941 ggcgcccgac ggcaatgccc tcgcggtgcg cggcgcctcg gtcgtcatca tcggggtcaa
     6001 gcccgcgatg gtgcccgaga cgctggccgc gctcgccggg gacctcgctc ccgacgcgct
     6061 ggtcatcagc gtcgccgcgg gagtcaccac ggcgacgatg gaggcgatcg tgagccagcc
     6121 ggttctgcgc gccatgccca acaccccctc cctcatcggc aagggcgtga cggggctcgc
     6181 cgccggttca cgtgccacag cggagcaggt cgccctcggc cgcgctgtct tcgagaccgt
     6241 cggtacggtc gtcgaggtgc cggagtcgca gatcgatgcc ctcagcacga tctcgggatc
     6301 gggacccgcc tacgtgttct acctcgtcga gcagctcacc cgcaccgcgg tgaatctcgg
     6361 cttcaccccg gcgcaggcgg ccgagctcgt caccggcacg ttcctcggct cggtggagct
     6421 gctcgtcgcc tccggcaggg agcccgagga gcttcgtcgc caggtgacga gcccgaacgg
     6481 cacgacccag cgcgcgatcg cagagctgga gaaggccgac ctgacaggtc tgttcgaccg
     6541 ggccacggca tccgctctcg cacgggcgaa ggagatggcc gagggcaggt aagcggattc
     6601 accgcccagt catagccggt ccataggaag cccgggcacc gtgtgatgag cgcgttcaag
     6661 cgcctcacat gcgaaggagt cctcgtggca accaagaagg aagagtcggc ccccgccgct
     6721 tccgccagca ccccgagtga gcccgtgctc gctcagtcag ccccggcaca gccgctcgtc
     6781 gcgccacgtc gtggcctcgg cggcggagcc attgccggca tcgtcgtcgg atcgctcgtc
     6841 gtcgccggtg ccctgttcgg tggcggagtg ctcgtcggca gccacctgcc gtcgggcatg
     6901 acccagagcc agttcgggcc cggattcggc ccgggtgacg gcgacaacga cggtcgtccg
     6961 ccgttcccgg gcggcggacc cggacccgtg cagcccggtc agaatgacgg cgacaccgac
     7021 tccaactaac cgaggtcagg tcgagccagg gtcaactcga cagggctgcg aacttctcga
     7081 tgtcggtcga gttgccgctc acgatgatca ggtcatgatt cgacacgatc gtctccggcg
     7141 tcgcgtaggt gaagtcggcc cccggcgact tcaccccgac gactgtcaca ccgaatttct
     7201 tgcgcaccaa tgactgtgac agcgggatgc cgcggatcgg cttgggcggg tacatcttga
     7261 cgatcgcgaa gtcgtcatcg aactcgatga agtccagcat gcgcccggag acgaggtggg
     7321 cgacccgttc gcccgcctcg gcctcggggt agatcacgtg gttcgcgccg atgcgggtca
     7381 ggatcgtgcc gtgcgacttg ctgatcgcct tcgcccagat ctgcgggatc ttcaggtcga
     7441 ccaggttggc cgtgatcagc acgctcgatt cgatggatga cccgaccgcg acgaccgcga
     7501 tcgagaagtc ctgcgcgccg atctgccgca gggcatcgat gctgcgggcg tcggcctgca
     7561 ccgcgtgggt gacccgctcc gaccacttct gaacgagacc ggcatcctcg tcgatcgcga
     7621 gcacctcccg atcgagtcgc tgcagctggc ccgccgtggc cgctccgaac cggccgaggc
     7681 cgatcacgag cacgggggcg tcgtgggcaa tcttctcaac caacgatggg cctctcttcc
     7741 ggttgtctga acagcttggg gtgctggctt gcggcgagcg ccgctgccag cgtgacggtg
     7801 ccgatgcggc cgaagaacat ggtcgctgcc agtacgtact gcgcggcatc cgtggtgttg
     7861 gcggtgaagc cggtcgacag gccgcaggtc gcgaaggcgc tgatcacgtc gaacagcacg
     7921 tggtcgagcg cggcatccgt catcatcatg atcacgatgc aggagacggc caccgtggtc
     7981 gcgccccaca gtacgacgct gacgccgagt cgcagcacgt cctgcgggat gcgccgctcg
     8041 taggcctcca tgtcgtcgcg accgcgggcc tcggcgaagg cggcgaggaa caggatcgcc
     8101 agggtcgtga ccttgatgcc gcccgcagtc gatgcggagc cgccgccgat gaacatcagc
     8161 atgtcggtga cgagaaggct cgagccgtcg agctgggaga tgtcgatcgt cgagaatccg
     8221 cccgagcggg ccatcgacga caggaagaag ctctgcagca cccgttgccc gccgtcgagc
     8281 ccgcggaacg cgtggtcgaa ctcgagcacg aagtaggcca gagcccccat cacgagcagc
     8341 agggcgaatg tgatgagggt gagcttgacg tgcagcgacc agcgccgcgg gttcctgagg
     8401 ttgcgcgaca gggcgtagat cacgggaaag ccgatcgccc ccgccacaac gccgagcatg
     8461 atgacgctga ggaaccagac gtcgcccgcg tagggtgcca ggccggtcga cgtcggcagg
     8521 aaccccgtgt tggtgaaggc catcgccgag atgtagacgg actgccacgc ggcatcccac
     8581 acgggcacac cctggatgag catgcggggg aagagcagga tcgcgacccc ggcctcgatc
     8641 acgagagcac tgatggccac ggtgcgcagc aggttgccgg tctcgccgag gcggatcgcc
     8701 tggccctccg cgatcggccc cttgcggatg cgcagcgggt tggcgtcgcc ggccgcgagc
     8761 agcttctgcc gcagccccag acgcctactg atcacgaggc cgaggatcga ggcgacggtc
     8821 agcacgccga caccgccgat ctcgacgccg aggaacacca gcacgtggcc gaacggcgac
     8881 cagtaggttg ccatgtcgac ggtggacagg ccggtgacgc aaatcactga caccgcggtg
     8941 aagagagcgt caaccagcgg cgcctgctta cccgagctcg tggcgatcgg cagcgagaag
     9001 agcgccgtga agacgaggat gagagacgcg aagatcacga tggcgaagcg agcggggctc
     9061 gaggccacga acgcatccac cacatcctgc agacgaccga acaccgttct gcgcgagacg
     9121 ggtctgccgc gcatgctcac ccgaccagta tcgcgcctca cgtgtgcgcc ggtagcctga
     9181 actcatggct gacatcttcg acgtgatcgc tgacccgacg agacgagacc tgcttcaggt
     9241 gcttctggag cgctatgtcg ctgccgactc cgagacgggg gagatcagcg tcggtgacct
     9301 cgtcgagcgg ctgggtctca gccagccgac ggtgtcgaag caccttcgcg tgctgcgcga
     9361 ccacggcctc gtgagcgtgc gggaggacgg ccagcaccgc tactaccggc tcgaggcgac
     9421 gccgctcgag gaactcgagg actggctgca gccctacctc agcgccgact tcgacgacac
     9481 cttcgagcct gccgacggtg gccccaacgc cttcgcggcc tggtcgggcg ccgatgtcgg
     9541 cgagacgatc ggtcgtcgag tcgccgaggg tactcaccag gcaagagtcg tgattcagga
     9601 tgccgcggag caggtcacca agcgcctgcc caagtcgttc cgccgcaggc cgcaccgccc
     9661 ctagtttccg gtggtcgagc gtatcgggac cggtgctcga gcgcgtcgag gccgacacct
     9721 gagaccagtt caggggctgg acaccgactc acatcagccc ctagtgttac cgattagcac
     9781 tccagtcaca ttccctcgag tgcgcaatcc tgggagcccg cagccgatgt cactgtcaga
     9841 cgttcgattc ctcaccgtcg ctgaggtggc ggagatgatg cgcgtctcga acatgaccgt
     9901 ctaccgtctc gtgcactccg gcgatctgcc tgctgtgcga ttcggtcgtt cgttccgtat
     9961 ccccgagtcc gcggtcgtcg cggccatcca gaccccgatc gccgacgtcg gctgaggcct
    10021 cactccgcac tgatgtaaga tcgcggggta tgtctttccg cggttctgaa tcggaccggg
    10081 tcggtccatt cggaaacaga aaaggttctc atgggttcag tcatcaagaa gcgtcgcaag
    10141 cgcatggcga agaagaagca ccgcaagctg ctgcgtaaga cgcgtcacca gcgtcgcaac
    10201 aagaagtaga tcgctgatcg agtagcccga agggcgtatc gaggtctggc tgcgatacgc
    10261 agggtttcga tacgcagggt ttcgatacgc gacttcgtcg ctactcaacc cgctttgcgg
    10321 tcccgcttga cctgcttgcg ggcatccgtg atgcttgacc gcttcagcgg cagcactcgc
    10381 cagccgtgag tgttggcgtg ggcgagtagc ttcgcgtcgg ggttcaccac gacccggttg
    10441 ccgaccatcg tcagtagcgg gatgtcgttg ctcgaatcgg agtaggccca gcactgagcc
    10501 agatcggccc gcttctgctc ggcgaacagt cgcgccactg tggccttctc gtcgccgtgc
    10561 agcactgggc cgtcgagctc gccggtgaac actccctcga gggcctgcac cttggtgccc
    10621 agggcgccgg tgagaccgag ccgctcggcg atcacctgag cgacgatctc cggagtcgcc
    10681 gtgatcagcc acacctcgtg gccctgggcg aggtgctcac gcgtgagcgc gacggtctcc
    10741 ggccagagat tgggcaggat gtccttctcg tagatgtcgt tggcgagcat gacgagggcc
    10801 tcttcggtgt gaccgccgac caggccgagc gctcgctcct tggcgtgcgc caggtgctgc
    10861 cggttctcgc cgacggcaag gaatcggaac tggtgccagg cgaacaccgc aatgtcgcgg
    10921 aacgagatca gccgacgacg ccacgcctcg cgacccacgt agaagacgct cgcgccacgc
    10981 atgagcgtgt tgtcgacgtc gaagaaggcc acgatgggcc ccggggtcgg ggtgcgccgc
    11041 gctgcatcag acatcgcgat tagtctatgg tcgtgcccga cgtacgcctc accctgctgt
    11101 ccaagccggg gtgtcacctg tgcgatgcgg cgcgggatgc cgtgaccgag gtcatcgcgc
    11161 agctcggggc agcggcatcc gcagtcaccg tcgaggagct gtcgatcctc gatgacccgg
    11221 agctgaacga gcgctactgg gacgagattc ccgtggtgct ggtcaacggg cgcgtgcaca
    11281 ccatctggcg ggtcgacccg gcgcgcctga cccgagccct cgaggaggca attcaatgat
    11341 ccgacacgtg gtgatgtgga agctcagcgc gaccgacgcg cccgccaaag ccgcggcgat
    11401 cgcggcgatc gccggtgccc ttgagccgct tgtcggcgtc gtccctgggt tgcagagcct
    11461 catggtgcgg ccgaacgtcg cctatctgga gaccaactac gacgcgattc tcgtcagcga
    11521 cttcgactcc gtcgaggcgc tcgagcagta tcaggtgcac ccggcgcatg tcgaggctgc
    11581 tgcggtgccg aggtcgcacg cggcatcccg cgtgacggtg gacttcgagg tttagcccgc
    11641 ggccttctgg gtctcgatcg gaccggtgag gctcttggcg agcttgtcgc gctcggcgct
    11701 gagcgaggcg agggtggttg cttcgaggcc aggctcgagc acatcggcga aggggttgcc
    11761 cagaccgctc ttcgcgtgaa cggcctcggc gtaggcggtc gcagctgcgg ccacccgagc
    11821 gcttgcgcgc tgcgatttct tcagtgacac agtgcggccg cccacggcct cggcgtgctt
    11881 gttcagggcc ttcttcaggt ccttgagtgc tgcatcgatc ttcttggaca tggggacatc
    11941 atgccccatc cagatgaacg tctggaagtg aggtcagctc tcgtcgacga cggcggcgtc
    12001 gggagcgttc ttgcgcaccg actcgattcc gttctgagcg cccgacttgc tggcgtaccc
    12061 ctcggacatc gcgatgatct ccccatttcc agccttgagg acgaagcgga actcgccctt
    12121 cttgtccttg gagataacga actttccagc catggatggc ccctttcgca gttgcggttg
    12181 cttgagggtc agcctagggc gctagggcag caggcgggta ggtccacgga acagatacgt
    12241 ggtctcgcgc aggttcggca ggtgcagcat gagcatcagc acccggctga gccccatgcc
    12301 gaagccaccg tgcgggggaa caccgtagcg gaagaagtcg aggtagaagc cgagctcctc
    12361 ggggtcgagc cccttgtcgc gagcctgtgc ctccaggatc tcgatgcggt gctcacgctg
    12421 tgctccggtg gagatctcga cgccgttgaa gatcaggtcg tagctgttgg tcagcgacgc
    12481 gtcatccgcg tgcctcatgt ggtagaacgg ccggatgctg gaggcgtagt ccgtgaggaa
    12541 cacgaactcg tggtcgaacg tctccttcac gtaggcggcg atctggcgct cgccctccgg
    12601 gtccatgtcg tcgtcgtggc ggggcacctc gtagccgcgc tcggcgacga tgcgcttggc
    12661 ctcggcgagc gggatgcgcg ggaacggcac cgtgggtacg gtcacctcga ccccgaacag
    12721 ggcctcgatc tcgtcgccgt gcttcgcctt gactgccgcg aagccggcca ccagcagctc
    12781 ctcgtggagc ttcatgacgt cttcgtgcga gtcgatccag ctgatctcgc tgtcgacgct
    12841 ggtgaactca gtggcgtggc ggctcgtgaa gctcgggtcg gcgcggaacg ccgggccgac
    12901 ctcaaagatc ttgccgaagc cggccggctg ggccatctgc ttgaagaact gcgggctctg
    12961 cgacaggtag gccttggtct cgaagtactc gacctcgaac agctcggcct tggattcgct
    13021 cgcgctggcc atgagcttgg gggtgtgcag ctcgatgaag tcgtgctcga cccagtaggt
    13081 gcgccaggcg tgctcgaggg tggtctggat gcggaagatc aggttgttct tcggctggcg
    13141 caggtcgagg aagcgccagt ccatgcgctt gtcgacgccg gtgtcgtcgg cgatgggcgt
    13201 ctcggggttg gctgcggcgg cgatgtcgag tgcctcgagc ttgatctcca ccccgccgag
    13261 cttcacgcgc tcgtcgtgc
//
LOCUS       DAOSOU010000031        13234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4716_length_13234_cov_6.487063, whole genome shotgun sequence.
ACCESSION   DAOSOU010000031 DAOSOU010000000
VERSION     DAOSOU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_13234_cov_6.487063"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLW15_02990"
     CDS             <1..829
                     /locus_tag="PLW15_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT33398.1"
                     /translation="LLNAGVPLRAPVAGIAMGLISDEVDGVTRYAALTDILGAEDALG
                     DMDFKVAGTSEFVTAIQLDTKLAGLPSSVLDGALKQAKEARTAILAVINAAIDSPDEM
                     APTAPRVISVQIPVDKIGELIGPKGKTINQIQDDTGADISIEDDGTVYIGAVDGPSAE
                     AARAAVNAIANPLNPEVGERFLGTVVKLATFGAFVSLTPGKDGLLHISEVRKLAGGKR
                     VENVEDVLSVGQKIQVEITKIDDRGKLSLAPVDDSADAADTDSSVAASDGPEAPAEG"
     gene            955..1134
                     /locus_tag="PLW15_02995"
     CDS             955..1134
                     /locus_tag="PLW15_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33399.1"
                     /translation="MTTPFDHFMKAFTDWLDTAWYLWVAMVVIFAVVVAVVVLSKVRA
                     RARQRVPQYRRQLPG"
     gene            1328..2332
                     /locus_tag="PLW15_03000"
     CDS             1328..2332
                     /locus_tag="PLW15_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT33400.1"
                     /translation="MPTPDTVPTPDTVPTTGETSAVVGQSAPRLRRVGESDLTVFPLA
                     ISGNVFGWTADDATTNAILDTYTGQGGNFIDTADSYAAGRSETMIGNWMRSRRNRSDV
                     VIATKVGKSADNPGLRARVLTRAVHASLQRLGTDRIDLLYLHIDDETVEFEETLLAVD
                     ELIRAGAVRYFGGSDHTGNRLIEARIASAQVGVAQMVALQNHYNLMHRDEYEGDLAHV
                     AAQQRLGVMPRFALASGFLTGKYRQKSDLAKGERGAEVARYLNRRGLRVLATLDTIAA
                     AHGTSVASIALAWLLTKPNVVAPVASASRPEQVRDLTAAAAVQLTRHQVSDLDRVSAG
                     "
     gene            complement(2360..2701)
                     /locus_tag="PLW15_03005"
     CDS             complement(2360..2701)
                     /locus_tag="PLW15_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33401.1"
                     /translation="MITLSWLLIVTVLAAALSMFDGIRRVRGNRSNSILAIAELVFAV
                     LMLISIFIAFPAPLGTFVFAVALEVVLILLLVLRGTRGSGFTTLTLIALILNTVVVLI
                     AAGWLSIPGLG"
     gene            complement(2772..3371)
                     /locus_tag="PLW15_03010"
     CDS             complement(2772..3371)
                     /locus_tag="PLW15_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT33402.1"
                     /translation="MVVEEAPTTQRAPAKGRILETANELFYEDGIRNVGVDRIISASA
                     VTKATFYKHYRAKDNLIVEYISARHVTVRESIEAMIASAETPEDALHLFVADIAAEIA
                     RPGFRGCPFINAAAEFPDADHPVRRIVTDHREWYVDTLALMLRNMGHPVPGDAADELL
                     LARDGALAGGYAGDSVAATAALGRITSRVLAEASPAKLA"
     gene            3583..4941
                     /locus_tag="PLW15_03015"
     CDS             3583..4941
                     /locus_tag="PLW15_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT33403.1"
                     /translation="MNGAVALPLELADLTFTASGNSLVRRTVLPSGVRILSEAVPGAR
                     SSTIGYWVAVGSRDEHPETFGSTHFLEHLLFKGTPSRSALDIAVAFDSVGGEHNAMTA
                     KEYTCYYAKVQDRDLPMAVDVLADMFTSSLIDPQEFETERGVILEELAMADDDPADVT
                     SERFFEAVLGEHPLGRPIGGNPATITAVSREAVVEHYRANYRAQDLVITAAGAVDHDV
                     LVAGVVAALERAGWDLGAEARPVERRDATHDLIERGSPLVVVNRPIEQANLIAGVVGI
                     AAADPRRATLSVLNSLFGGGMSSRLFQEIREKRGLAYSVYSFTPSFSDSGLFGIYAGC
                     SPAKAPQVAELMLGEFARLAAHGASDDEVRRAVGQLSGASALALEDSDTRMSRLGRSE
                     ITLGEFVDLDESLRRLAAVTADDVQALAAELAERPLSIAAVGTVDASHFSGLAVPPVS
                     IP"
     gene            4974..5576
                     /locus_tag="PLW15_03020"
     CDS             4974..5576
                     /locus_tag="PLW15_03020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT33404.1"
                     /translation="MAHYLYLVRHGEQQDAEHGLPDGPLSPKGQRQARAIADRLGGVP
                     FSRAWHSPLERAAQTAAIMTERMPALTSTPSSLLMDCIPSGPTPDMPHAFEPFFGSVT
                     PEQIEAGEAQMADAVAEFLAPAMEDRHDLLITHNFVIAWFVREVFGAPSWRWLGLNQA
                     NCGLTIIRVRSAKPPVLVTHNDLGHLPAELRTGLPQEQPF"
     gene            complement(5573..6028)
                     /locus_tag="PLW15_03025"
     CDS             complement(5573..6028)
                     /locus_tag="PLW15_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33405.1"
                     /translation="MPTYRATPVTDATAHALLTEYFAYRASTFPAGSQYRVTHPDPAR
                     FVAPDGVFLVVREFGDALGCGGIRRLENARFEVKHLWLRPLARGRGLGRALLNELEKR
                     AKRLGATELVLDTNTSLEAAGELYRTSGYESIDAYNDNPNATDWYRKAL"
     gene            complement(6073..7695)
                     /locus_tag="PLW15_03030"
     CDS             complement(6073..7695)
                     /locus_tag="PLW15_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT33406.1"
                     /translation="MELWEAVGAEWDRALSGDVTTAFETASRLLDSRESPDDRAVLLA
                     FRAFCELTSCDYLAGALTAEAAALESPTGQLARFYVDAVRLLAAGMVDQGTDAATLPL
                     SFDEVMARSAAVLPLGADAQLAVHPLIEAAMATGRFVVVEQLVNTHPATGVGSSTQVG
                     TWVRLQLIRSQLFRGQLPDVVAGCAALESEGALARHPQAAMLANALLCYAAAQSGDRV
                     EVDARSTRVLAEARRVGTYVQVGSCLLVAWSFSTIGQVQRAAALLLGTAGGPGLMRIK
                     TWDRAFGYELLASAALQRGDLAAAREWAAWAAPLARYEVAAAAVERTLSKVAAAEGDS
                     GEALARAIRSAAHDSATGSRIDELRSRVLVAGALAARGEHGEAIANLGETAREAERLG
                     ADAVRREASRELRALSDRRGGWESLTDREREIAVLAAEGHSNRTIASTLFISDRTVQS
                     HVSRALRALGVDSRTGIPSIVTTATRIEAPPLTDRQQQVARLVASGMSNAAIAAELGI
                     SAKTVEKHLSGIFERWGVSSRTAIATRELARS"
     gene            7749..8474
                     /gene="dapB"
                     /locus_tag="PLW15_03035"
                     /pseudo
     CDS             7749..8474
                     /gene="dapB"
                     /locus_tag="PLW15_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
     gene            8489..8929
                     /locus_tag="PLW15_03040"
     CDS             8489..8929
                     /locus_tag="PLW15_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33407.1"
                     /translation="MTARIGAIVMAALLGLYLVFVFQYALLLIGSQNGIAKALGIALL
                     VLPIVGAWALVSEIVFVVRGQRLVRRLGEQGGLPVDDLPRLPSGRIDPQAADLEFPRY
                     RAEVEASPDSWQAWLRLGLAYDASGDRGRARWATRAAIRLERRR"
     gene            complement(8907..9827)
                     /locus_tag="PLW15_03045"
     CDS             complement(8907..9827)
                     /locus_tag="PLW15_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOT33408.1"
                     /translation="MAAARPTPLRVLVSGASGFIGAELCRQLEADGHTVLRLVRRAPT
                     GPNEFRWSPSEKTVDAAAIESADAVINLAGATTGRIPWTRAYKREILYSRVNGTTTLA
                     EAIRASSNPPTIFLNGSAVGYFGDQPGVSLDESGAKGEGFLSDVVQAWEKAAHLAPPT
                     TRVVTFRTGLVVGRGGAFTPLALLTRVGLGARIGTGRQNWPWISLHDEAAAIRHLLTS
                     SLTGVVNLAGPTPATSVQVTRALATALRRWHLLVVPTFALRLLGDAGEDLLLTSEKVV
                     PAKLLADGFVFRDATIEKAVAAEFSDAARV"
     gene            complement(9827..10252)
                     /locus_tag="PLW15_03050"
     CDS             complement(9827..10252)
                     /locus_tag="PLW15_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HOT33409.1"
                     /translation="MSVTSEATTLWFGDLLSGSGTTSLDSSDAAEFPVTWAARSEGVS
                     GQTNPEELLGAAHSACYSMAFSNALAKNGTPPESLQVTAAVTFEPGTGITGSHLLVSA
                     KIAGISEDDFQRLAAEAKSGCPVSQALAGIPITLEASLA"
     gene            complement(10272..10796)
                     /locus_tag="PLW15_03055"
     CDS             complement(10272..10796)
                     /locus_tag="PLW15_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOT33410.1"
                     /translation="MTTTKPTPAAKPGQKGIDPRSPRFGAGITAVLLLVVVGLGLVTP
                     FGATVAERATEPGLILFALIWALFGWGAFAGVQRHPYGAIYRALIRPRLAPPTELEDP
                     VPPTFAQGVGFVITLIGIVLHLAGVPYALVVAAGAAFVAAFLNSVFAYCLGCQIYLLL
                     VRAGILGRKGAATA"
     gene            complement(10831..11262)
                     /locus_tag="PLW15_03060"
     CDS             complement(10831..11262)
                     /locus_tag="PLW15_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT33411.1"
                     /translation="MNPVAVVGVLVGLVALATVIGLVWRSQQGRIHHTRGTTTVKVGD
                     VPGMRRFARGATFLQFSTEVCAPCKVTRDVLGAVANEHSNVAHVDVDLTHRPDLASQF
                     HIMQTPTTLILDSKGVVRARIGGAPRRDAVRAELDRILVAA"
     gene            11346..12083
                     /gene="thyX"
                     /locus_tag="PLW15_03065"
     CDS             11346..12083
                     /gene="thyX"
                     /locus_tag="PLW15_03065"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOT33412.1"
                     /translation="MPDDKPAVKFRSDVTVELVRSSASDSDVLFAARVSTQGEQTLES
                     AQDENLDASRSKGLINYLMRDRHGSPFEHNSMTFYVQAPIFVFREFMRHRIASYNEES
                     GRYRELNPVFYVPGPDRNLVQVGKAGAYDFLPGSPEQTELAVSESKAQATAAYESYQR
                     MLDAGIAREVARIVLPLSIYSSMYVTMNARSLMNFLSLRTKREGTHFPSFPQREIEMA
                     AEQMEALWADLMPLTYATFNENGRVAP"
     gene            complement(12775..>13234)
                     /locus_tag="PLW15_03070"
     CDS             complement(12775..>13234)
                     /locus_tag="PLW15_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT33413.1"
                     /translation="RVSITSPYFVPDESTLMAIVTAAARGLDVELFVSAIGDQHLVYH
                     AQRSYYEALLRAGVKIYLYRAPTVLHSKHFTIDDDVAVIGSSNMDVRSFSLNMEVSVM
                     VQGESFVSAMRAIEDDYRASSDLLTLEEWMTRPLAGKVNDALARLTSSLQ"
BASE COUNT         2166 a         4433 c         4512 g         2123 t
ORIGIN      
        1 gctgctcaac gccggtgtgc cgctgcgcgc gccggtcgcc ggcatcgcga tgggtctcat
       61 ctccgacgag gtcgacgggg tcacgcgcta cgcggccctc accgacatcc tcggtgcgga
      121 ggacgcgctc ggcgacatgg acttcaaggt cgccggtacc agtgagttcg tcaccgccat
      181 ccagctcgac acgaagctcg cgggtctgcc ctcgagcgtg ctcgacggtg ctctcaagca
      241 ggccaaggag gctcgcaccg cgatcctcgc cgtgatcaac gccgcgatcg actcgcccga
      301 cgagatggcg cccacggcgc cccgcgtcat cagcgtgcag atcccggtcg acaagatcgg
      361 tgagctgatc ggcccgaagg gcaagacgat caaccagatc caggatgaca ctggcgccga
      421 catctcgatc gaggacgacg gcaccgtgta catcggcgct gtcgatggtc cctcggccga
      481 ggcggcccgc gcggcggtca acgcgatcgc gaacccgctc aacccggagg tcggcgagcg
      541 gttcctcgga accgtcgtca agctcgcgac gttcggcgcg ttcgtctcgc tcaccccggg
      601 caaggacggc ctgctgcaca tcagcgaggt gcgcaagctc gccggtggaa agcgcgtcga
      661 gaacgtcgag gacgtgctct ccgtgggcca gaagatccag gtcgagatca cgaagatcga
      721 tgaccgtgga aagctctcgc tggccccggt cgacgacagc gcggatgccg cggacaccga
      781 cagctcggtt gccgcgtccg acgggcccga ggcacctgcc gaaggctagt cgctagcgat
      841 ccatcactca ggcccgcccc ggaaacgggg tgggcctgag tcattctcgg caactcctgt
      901 ctgcccccac tttgggggta aggaggcaag cgattgcact cctagggtga gcgagtgacc
      961 acacccttcg accacttcat gaaggcgttc accgactggt tggacaccgc gtggtacctc
     1021 tgggtcgcca tggtcgtgat cttcgccgtg gtcgtcgccg tggtggttct gtcgaaggtg
     1081 cgggcccgtg cccgccagcg ggttccccag tatcgccgtc agctgccggg atgactcagc
     1141 gcctcattcg cgcatagggc gccctctgcc cactcgggcc gtcccgcatg cgccggacga
     1201 ggaaacgacc tcgtaacgat tgggcatttc tgaccgactc gcgcgctcgt gcgacgtagt
     1261 ctgaagatgc cttggggagg cgagaatgat tcagttggat acgagcacag cgctccaagg
     1321 acagacagtg cccacgccag acacggtgcc cacgccagac acggttccca cgacaggtga
     1381 gacctcggca gtagtcgggc agtccgcgcc ccgactgaga cgagtcggtg agtcggacct
     1441 gacagtcttc cccctggcca tcagcggcaa cgtcttcggc tggaccgccg acgatgcgac
     1501 gaccaacgcc atccttgaca cctacaccgg gcagggcggc aacttcatcg acaccgctga
     1561 ttcttatgcc gcgggtcgca gcgagacgat gatcggcaac tggatgcgct cccggcgcaa
     1621 ccgctccgat gtcgtcatcg ccaccaaggt gggtaagagc gccgacaacc cgggccttcg
     1681 cgcacgggtg ctcacgaggg ccgtgcacgc gtcccttcag cggttgggaa cagatcgcat
     1741 cgacctgctg tacctgcaca tcgatgacga gacggtcgag ttcgaggaga ccctgcttgc
     1801 ggtcgatgag ctgattcggg cgggtgccgt gaggtatttc ggcggctcag atcacacggg
     1861 aaaccggttg atcgaggcgc gaatcgcatc ggcacaggtc ggggtcgccc agatggtcgc
     1921 cctgcagaat cactacaacc tgatgcaccg cgacgagtac gagggcgatc tcgcccatgt
     1981 cgcggcccag cagcggctgg gcgtgatgcc tcgattcgcc ctcgccagcg gcttcctcac
     2041 gggtaagtac cgccagaagt ccgacctcgc gaagggggag cgcggggcgg aagtcgcccg
     2101 ctacctgaac cgtcgcggac ttcgcgtgct cgccactctc gacacgatcg cggctgcgca
     2161 cgggacgagc gtggcgtcga tcgccctggc gtggctgctg acgaaaccga acgtcgtggc
     2221 accggtggcc tctgccagcc gcccggagca ggtgcgcgac ctcacggctg ctgcggcagt
     2281 gcagttgacc cgccatcagg tgtcggacct ggatcgggta tcggccggct agccctgcgg
     2341 gcgaaccggc cgacgaccgc tagcccaagc cgggaatgga gagccatccc gccgcgatca
     2401 gcacgaccac ggtgttgaga atgagtgcga tcagcgtgag cgtggtgaag ccgctgccac
     2461 gagttcccct cagcacgagc agcagaatca gcacgacctc gagcgcgaca gcgaacacga
     2521 aggttcccag cggcgcggga aacgcgatga agatcgagat gagcatcagc accgcgaaca
     2581 ccagctcggc aattgcgaga atggagttgc tgcggttgcc tcgcacgcga cgaattccgt
     2641 cgaacatcga gagggcggcg gcgagcaccg tgacgatgag aagccaggag agtgtgatca
     2701 tgcccatcac cctaggggga ggtgccgggg cgacctccgg ggcctagcca gcgcccgcgc
     2761 gcgccgctac actacgcgag cttggccggg gatgcttcgg ccagcacgcg actggtgatg
     2821 cgacccagag cggctgtcgc agccaccgag tcgcccgcgt acccgccggc gagcgcgcca
     2881 tcgcgcgcga ggagaagctc gtcggccgcg tcgcccggaa cgggatgtcc catattgcgc
     2941 agcatcaggg cgagagtgtc gacgtaccat tcccggtggt cggtgacgat cctgcgaacc
     3001 gggtggtcgg catccgggaa ctcggcggcg gcattgatga aggggcagcc gcggaatccg
     3061 ggccgagcga tctcggcggc gatgtcggcg acgaagaggt gcagagcatc ctccggcgtc
     3121 tcggccgagg cgatcatggc ctcaatggac tcgcgaaccg tcacgtgacg agcgctgatg
     3181 tactcgacga tcaggttgtc cttggcgcgg tagtgcttgt agaacgtcgc cttcgtcacg
     3241 gccgacgcgc tgatgatgcg atcgacgccg acattgcgga tgccgtcctc gtagaagagc
     3301 tcgttcgctg tctcgaggat gcggcccttc gctggagcgc gctgtgtggt cggagcttcc
     3361 tcaacgacca tcacagcgcg actccttgcg ggggtttccc gatcgtgggg gacgatcggg
     3421 gtggtgcacg accgccatcg ggttagcggc tgtgcggggc ttcgggtgcc cgatagaaaa
     3481 tatagcacat gaggacagac agaccagtct gtttgtcctc atgtgtgtgc ccagttcggg
     3541 gggtaggcca tccgcgcacc cggatgacgt aggctcgatc cgatgaacgg cgccgtagct
     3601 ctcccgctcg aactcgcgga cctgacgttc accgcatccg gtaactcgct cgtgcgacga
     3661 acagtgctgc cgagcggtgt gcgcattctc agcgaagcgg tccccggggc gcggagttcc
     3721 accatcggct actgggttgc cgtgggctcg cgggacgagc atccggagac cttcggctcg
     3781 acgcactttc tcgagcacct gctgttcaag ggcaccccga gccgcagtgc tctcgacatc
     3841 gccgtcgcct tcgactcggt cggcggcgag cacaatgcga tgaccgccaa ggagtacacc
     3901 tgctactacg cgaaggtgca ggaccgcgac cttccgatgg ccgtcgatgt tctggccgac
     3961 atgttcacgt cctcgctgat cgacccgcag gagttcgaga ccgagcgcgg ggtgatcctc
     4021 gaggagctcg cgatggccga cgatgacccg gccgatgtca cgagcgagcg cttcttcgag
     4081 gcggttctcg gcgagcaccc cctcggtcgc cccatcggcg gcaacccggc cacgatcaca
     4141 gcggtgagtc gcgaggcggt cgtcgagcac taccgggcca actaccgcgc gcaggatctc
     4201 gtgatcaccg ccgccggcgc cgtcgatcat gacgtgctcg ttgccggggt cgttgctgcg
     4261 ctcgagcgag cggggtggga tctcggggcc gaggcgcgcc ccgtcgagcg ccgcgatgcg
     4321 acacatgacc tcatcgagcg ggggagccct ctggtcgtcg tgaaccgacc gatcgagcag
     4381 gccaacctca tcgccggtgt cgtcggcatc gcggcagcgg acccgcggcg ggcaaccctg
     4441 tcggtgctga actcgctgtt cggcggcgga atgtccagtc ggctattcca ggagatcaga
     4501 gagaagcgcg ggctcgccta ctccgtctac tccttcacgc cgagtttctc ggattccggg
     4561 ctgttcggca tctatgccgg atgctcgccc gcgaaggccc cgcaggtcgc cgagctcatg
     4621 ctcggcgagt tcgcgcggct cgcggcccac ggcgcgagtg acgacgaggt gcgacgcgcc
     4681 gtgggacagc tcagcggggc atccgctctg gcgctcgagg actccgacac gcgcatgtcg
     4741 cgcctcggcc gcagcgagat caccctgggc gagttcgtcg atctcgatga gagcctgcgt
     4801 cggctcgctg ccgtcaccgc tgacgatgtg caggctctgg ctgccgagct ggccgagcgt
     4861 ccgttgtcca tcgccgccgt cggcaccgtc gacgcctcac acttctcggg actggcagtg
     4921 ccgccagttt cgatcccgta acacagccac acgaaacacg aggtgccagt cacatggctc
     4981 actatctcta tctggtgcgt cacggcgagc agcaggatgc cgaacacggg ctccccgacg
     5041 gaccgctctc gcccaagggc cagcggcagg cgcgcgccat cgccgaccgg ctggggggag
     5101 tcccattcag ccgcgcctgg cactcgcccc tggagcgggc agcgcagacg gccgcgatca
     5161 tgaccgaacg gatgcccgcc ctcacctcca caccgagctc gttgctgatg gactgcattc
     5221 cgtccgggcc gacccccgac atgccgcacg ccttcgagcc cttcttcgga tcggtcactc
     5281 ctgagcagat cgaggcgggc gaggcgcaga tggccgacgc ggtcgcggag ttcctcgccc
     5341 cggcgatgga ggatcgccac gacctgctga tcacgcacaa cttcgtgatc gcatggttcg
     5401 tgcgcgaagt gttcggggcg ccctcctggc gctggctggg gctgaatcag gccaactgcg
     5461 gcctgacgat catccgggtg cgcagtgcga agccgcccgt gctcgtcact cacaacgatc
     5521 tcgggcacct tcccgccgaa ctacgcaccg gcctgccgca ggagcagccc ttctaaagcg
     5581 ccttgcggta ccagtcggtc gcgttcgggt tgtcgttgta cgcgtcgatg ctctcgtagc
     5641 cactcgtgcg gtagagctcg ccggcagctt cgaggctcgt gttcgtgtcg agcaccagct
     5701 cggtcgcgcc gaggcgcttc gcgcgcttct ccagttcgtt cagcagcgct cgacccagac
     5761 cgcgacctcg ggccagtggc cgcaaccaca ggtgcttgac ctcgaaacga gcattctcga
     5821 gacgacggat gccgccgcac ccgagcgcat cgccgaactc acgcaccacc aggaacacgc
     5881 cgtcgggcgc cacgaacctc gcgggatccg gatgggtcac gcggtactgg gaccccgcgg
     5941 ggaaggtgga cgcgcggtag gcgaagtact cggtgagcag ggcgtgcgcc gtggcatccg
     6001 tcaccggggt ggcgcgatac gtgggcacgt gttcagcgta gaacctcgga cgccttcgag
     6061 tgaggctgcc gatcagcttc gcgcgagctc gcgcgtcgca atggccgtgc gcgacgagac
     6121 tccccaccgc tcgaagatgc ccgacaggtg cttctccacc gtcttcgcgc tgatgcccaa
     6181 ctcggcagcg atcgccgcgt tcgacatacc gctcgccaca agccgggcca cctgctgctg
     6241 gcgatcggtc agcggtgggg cctcgatccg ggtggccgtg gtgacgatcg acgggatgcc
     6301 cgtgcgcgag tcaaccccca gcgctcgcag tgcgcgcgag acatggctct gcactgtgcg
     6361 atcggagatg aagagggtgc tggcgatcgt gcggttgctg tggccctccg ccgcgaggac
     6421 cgcgatctca cgctcgcggt cggtgaggct ctcccacccg ccgcggcgat cgctgagggc
     6481 cctcagctcg cgggatgcct ccctgcgcac ggcgtcggca ccgaggcgct cggcctctcg
     6541 cgccgtctcg cccaggttgg caatcgcctc gccgtgttcg ccgcgtgccg cgagtgcgcc
     6601 cgccaccaga acgcgagatc gcagctcgtc gatgcgcgaa cccgttgcag agtcgtgtgc
     6661 agcggacctg attgcccgcg ccagcgcttc gcccgagtcg ccctcagcgg ctgcgacttt
     6721 gctcagtgtg cgctccaccg ctgccgcagc cacttcgtag cgggcgaggg gcgcggccca
     6781 ggccgcccac tctcgtgctg ccgcgaggtc accgcgctgc agcgcggcgc tcgccagaag
     6841 ctcgtagccg aatgcgcgat cccaggtctt gatgcgcatc agccccggac cgccggccgt
     6901 gcccagcaga agtgctgccg cgcgctgcac ttgcccgatc gtggagaacg accacgccac
     6961 cagcaggcac gagcccacct gcacgtacgt gcccacgcgt cgcgcttcgg cgagtacccg
     7021 ggtgctgcga gcatccacct cgacccggtc cccgctctgt gcggccgcat agcagagcag
     7081 tgcgttagcg agcatcgcag cctgcggatg ccgcgccagc gcgccctcgg actccaacgc
     7141 cgcacatccc gcgacgacgt cgggcagctg cccccggaac aactgcgacc tgatcaactg
     7201 caggcgtacc caggtgccga cctgagtact cgagcccaca cctgtcgccg ggtgagtgtt
     7261 cacgagctgc tcgaccacga cgaaacgccc cgtggccatg gctgcctcga tgagcgggtg
     7321 cacggccagt tgtgcgtcag cgcccaacgg gagcacagcg gcggagcgcg ccatgacctc
     7381 gtcgaaactg aggggcaggg ttgcggcatc cgtgccctga tcgaccatgc cggccgccaa
     7441 caggcgcacg gcgtcgacgt agaagcgcgc aagttggccg gtcggtgact ccagcgccgc
     7501 tgcctcggcg gtcagggctc cagcgaggta gtcgcacgag gtcagctcgc agaaggcgcg
     7561 aaaggcgagc agcactgcgc ggtcgtcagg gctctcgcgt gagtcgagga ggcggctcgc
     7621 ggtttcgaag gccgtggtca catcgccgga gagggcacgg tcccattcgg cgccgactgc
     7681 ctcccacagc tccattcagc catgctacct aggccgcgca ctgtgcccgg cgttggtaga
     7741 tttgcgggat gacaacgacg gtcgccctcg cgggcgcgac gggccgcatg ggcacgctca
     7801 tcagcgatct gatcgccaag tcgggtgact tcgaggtggt ggcgcgcttg gactcgtcca
     7861 gcgcgctcga cgcgatgacc ggcgcagaca tcgtcgtgga cgtgacagtt cccgctgtgt
     7921 cgcagcgaat cgtcgagttc gccattgccg gcggtcagag cgtcttggtc ggtacctccg
     7981 gctggtcgtc ggaacggatc gcggcgcttc agcctctggt ggatgccaat ccctcgtcgg
     8041 gcgtcgtgat cgtcccgaac ttctcgatcg ggtcggtgct cgcgacatcc ttcgccgcga
     8101 cggcggcgcg ctacttcaac tcgatggaga tcatcgaggc tcaccacgcg acgaaggtgg
     8161 actccccgtc aggcacggcg gtgcgcaccg ccgaattgat gctcgcctcg cggggcgaac
     8221 tggggccggt cgcggccccc cacgtcgacc agcgggcgcg cggccagcag gtgtccagca
     8281 tcccgattca cagcctgcgg atgcacggtg tggtcgccaa gcaggacgtc atactgggcg
     8341 gtaccggcga gctgctcacc atcagtcacg agacgctgtc gcccagttcc tacgaggcgg
     8401 gaatcatgct ggcgctgcgt gccgtatcga gtgtgcaggg catcgtcgtc ggactcgatc
     8461 gcatcatcga cctggcggca gccgccgaat gacggccaga atcggcgcga tcgtcatggc
     8521 cgcgctgctc gggctgtatc tggtcttcgt ctttcagtac gccctgctgc tcatcgggtc
     8581 ccagaacggt atcgccaagg cgctcggcat tgcgctgctc gtccttccga tcgtcggagc
     8641 gtgggcgctg gtgtcggaga tcgtcttcgt cgtgcgcggc caacgtctgg ttcgtcgcct
     8701 gggcgagcag ggcggccttc ccgtggatga cctgccgcgc ctgcccagtg gccgcatcga
     8761 cccgcaggcc gccgacctgg agttcccgcg gtatcgggcc gaggtcgagg catcgcccga
     8821 ctcatggcag gcgtggctgc gcctcggact ggcgtatgac gcgagcggtg accgcggcag
     8881 ggctcggtgg gcgactcggg cggcgatcag actcgagcgg cgtcgctgaa ctcggcggcc
     8941 acggctttct cgatcgtcgc atcccggaac acgaagccat cggccagaag cttggccgga
     9001 acgaccttct cgctcgtcag gagcagatcc tcgccggcgt ctcccagcag gcgcagggcg
     9061 aaggtgggga ccacgagcag gtgccatcgg cgcagcgccg tcgcgagcgc gcgggtgacc
     9121 tgcactgagg ttgccggcgt gggtccggca aggttcacaa cgcccgtcag gctggaggtc
     9181 agaaggtggc ggatggccgc cgcttcgtcg tggaggctga tccacggcca gttctgccgc
     9241 cccgtgccga ttctcgcgcc cagaccgacc cgggtcagca gggcgagcgg cgtgaacgct
     9301 ccgccgcgac cgacgacgag gccggtgcgg aaggtgacga ctcgtgtcgt cggcggcgcg
     9361 aggtgtgccg ccttctccca ggcctgcacg acgtcgctga ggaatccctc gcccttcgcg
     9421 ccagattcgt cgagggacac cccgggctgg tcgccgaagt atccgaccgc agacccgttg
     9481 aggaagatgg tcggcggatt gctcgaggca cggatcgcct ccgcgagggt cgtggtcccg
     9541 ttgacccgcg agtagaggat ctcgcgcttg taggcgcgag tccacgggat acggccggtc
     9601 gtggcgccgg cgaggttgat caccgcgtcg gccgattcga tggccgcggc atccacggtc
     9661 ttctcagagg gagaccagcg gaactcgttc ggcccggtgg gcgcgcggcg cacgagccgc
     9721 agcaccgtgt ggccgtcggc ctcgagctgt cgacacagtt ccgcaccgat gaatccggat
     9781 gccccggaca ccagaacgcg caggggagtg ggccgcgcgg ctgccactag gcgaggctgg
     9841 cttcgagggt gatcggaata ccggcgaggg cctgggacac cgggcatccg gacttggcct
     9901 cggccgcgag gcgctggaag tcgtcttcac tgatgccggc gatcttggcg ctgacgagca
     9961 ggtggcttcc ggtgatgccc gtgcccggct cgaacgtgac ggccgccgtg acctgcaggc
    10021 tctccggagg ggttccgttc ttggccagcg cgttggagaa cgccatcgag tagcacgccg
    10081 agtgggcggc gccgagcagt tcttcggggt tggtctgccc ggagacgccc tcggaccgcg
    10141 cggcccacgt gacggggaat tcggccgcgt ccgacgagtc cagggacgtg gtgcccgatc
    10201 cgctcagaag gtcgccgaac cacagggttg ttgcttcgct ggtgactgac atgcctccag
    10261 actaggcggg ctcaggctgt agcggcaccc ttgcggccga ggatgccggc gcgcacgagc
    10321 aggaggtaga tctggcaccc gaggcagtag gcgaagaccg agttgaggaa ggcggccacg
    10381 aacgccgcgc cggcagcgac gacgagggcg tagggcacgc cggccaggtg cagcacgatg
    10441 ccgatcaggg tgatgacgaa tccgacaccc tgggcgaagg tcggcggcac cgggtcctcg
    10501 agctcggtgg gcggcgcgag acgggggcgg atgagtgcac ggtagatcgc gccatacggg
    10561 tggcgctgca cgccggcgaa ggcgccccag ccgaacagtg cccagatgag ggcgaagagg
    10621 atcaagcccg gctcggtcgc tcgctcggcg accgtcgcgc cgaagggcgt caccagcccg
    10681 aggccgacca cgacaagcag gagcaccgcg gtgatgccgg cgccgaagcg ggggctacgc
    10741 gggtcgatgc ccttctggcc cggcttggcg gcgggggtgg gcttggtggt tgtcatggtg
    10801 cctccggggc gggcgggaag aggggcaggg tcaggcggca acgaggatgc ggtcgagctc
    10861 agcgcgcacc gcgtcccgcc ggggagcgcc accgatgcgg gcacgaacga cgcccttgct
    10921 gtcgaggatg agcgttgtcg gcgtctgcat gatgtggaac tggctggcga gatcgggccg
    10981 gtgcgtgagg tcgacgtcga catgcgccac gttgctgtgc tcgttcgcaa ccgcgccgag
    11041 cacgtcgcgt gtcaccttgc agggggcgca gacctcggtc gagaactgca ggaaggtcgc
    11101 acctcgggcg aaccgacgca ttccggggac gtctcccacc ttgaccgtgg ttgttccgcg
    11161 tgtgtggtgg atgcgtccct gctgcgaacg ccacaccagg ccgatgaccg tcgcgagcgc
    11221 gacgagcccc acgagcacgc ccaccaccgc cacagggttc atggttgcca cgataggcgg
    11281 gcgggcaggg gtacgcccga gtgttgagaa ctgttacatc ccgcttctca gctatcgtga
    11341 aggccgtgcc tgatgacaag cccgccgtga agttccgatc cgatgtcacc gtcgagctgg
    11401 tgcgctcgag cgcgagcgat tcggatgtgc tgttcgccgc ccgcgtgtcc acccagggcg
    11461 agcagacgct ggagtctgcc caggacgaga acctcgacgc gagccgcagc aaggggctca
    11521 tcaactacct catgcgcgac cgccacggct cgccgttcga gcacaactcg atgaccttct
    11581 acgtgcaggc gccgatcttc gtgttccgcg agttcatgcg acaccgcatc gcctcgtaca
    11641 acgaggagag tgggcgctac cgcgagctca acccggtgtt ctacgtgccc ggccccgacc
    11701 gcaacctcgt ccaggtcggc aaggcgggcg cctacgactt cctgcccggc agccccgagc
    11761 agaccgagct cgcggtctcc gagtcgaagg ctcaggcgac cgccgcctac gagtcctacc
    11821 agcggatgct cgacgccggc attgcccgcg aggtcgccag gatcgtgctg ccgctgagca
    11881 tctactcgtc gatgtacgtg acgatgaacg cgcgatcgct catgaacttc ctctcgctgc
    11941 gcacgaagcg ggagggaacg cacttcccgt cgttcccgca gcgggagatc gagatggccg
    12001 cggagcagat ggaggcgctg tgggccgacc tcatgccgct gacctacgcg accttcaacg
    12061 agaacggtcg cgtcgcgccg tagctgtagc tgtagctgta gctgtagctc tcgctcgccg
    12121 caatcgacgg ccgggcctag ccgcaaactc ggtcgactcc ttcccgcgcc ttctgttctg
    12181 caggagaacg aaggaactct cgtgtctcct gcacaacgcc ggcagcggcc tgcttctgca
    12241 ggataaggga gtcgtcgtcg cctctcctgc acaacagaag ggttcagcat cagtcgccgg
    12301 atattccggc tcccgccgcc agcgcgccgc cgagcgcaca ccatggcgcc atggagatga
    12361 agctcgaacg cgacatcggc gccctgggag gaattgcaaa actctccgag ctactggaca
    12421 ggggccacca ccccgagcac gttcgaattg cgtgcaactc tggccgcatc atccgggtgc
    12481 gcaaaggctg gtacgcccga cgggacgcac cgtctgacgc catactcgcc tggcgagtgg
    12541 gcggccgcct cgcgtgcgtc agcgccttgc actacctcgg cctgctcgat cggcccgctg
    12601 ccctgcatgt cgctgtccgc gcgaacgcct ctcggctcag agctccgcgc aacgcgcgat
    12661 ctcggttgga tgcgctgtca gcggaagttg tcgtgcattg ggtctcagac gcgggcggtg
    12721 accgggtcac cgtcgccccc gcgaccgcac tcgcacaggc cgcgcagtgc tgggctactg
    12781 caacgacgac gtcagccgcg cgagggcgtc gttgacctta ccggcgaggg gccgcgtcat
    12841 ccactcttcg agtgtcagca gatcgctgga ggctcggtag tcgtcctcga tcgcgcgcat
    12901 cgccgagaca aagctctcgc cctgaaccat gaccgatacc tccatgttga ggctgaacga
    12961 gcgcacgtcc atgttgctcg acccgatcac cgccacgtcg tcgtcgatcg tgaagtgctt
    13021 ggaatgcagc actgtcgggg cccggtacag gtagatcttc acgccggcgc gcaggagcgc
    13081 ttcgtagtag gagcgctgtg cgtggtagac gagatgctga tcgccgatgg ccgagacgaa
    13141 gagctcgacg tcgaggccgc gggcggccgc cgtgacgatc gccatgagcg tcgactcgtc
    13201 aggcacgaag tagggactcg tgatgctcac cctg
//
LOCUS       DAOSOU010000032        13190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4727_length_13190_cov_6.052988, whole genome shotgun sequence.
ACCESSION   DAOSOU010000032 DAOSOU010000000
VERSION     DAOSOU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4727_length_13190_cov_6.052988"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLW15_03075"
     CDS             <1..256
                     /locus_tag="PLW15_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT33414.1"
                     /translation="HQRDRMILALPALGYTPLPSAANFVLFGGVADPHAVFEALLERD
                     IIIREVGIPHHLRVTAGSDAETTLFLDALGQLESIGRMAP"
     gene            253..858
                     /gene="hisB"
                     /locus_tag="PLW15_03080"
     CDS             253..858
                     /gene="hisB"
                     /locus_tag="PLW15_03080"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOT33415.1"
                     /translation="MTAGRTASISRTTSESSIELSLDLDGTGTSSISTSVPFFDHLLT
                     AFSKHSLVDLTVTATGDTHIDVHHTVEDVGIALGTAIRQALGDKAGISRFGDALVPLD
                     EALVQAVVDVSGRPYLVHTGEPAGFEHHLIGGHFTGSMLRHVFEAITFNAGLTVHITV
                     LGGRDPHHIAEAEFKAFARAFRLAVQPDARVSGIPSTKGAL"
     gene            861..1496
                     /gene="hisH"
                     /locus_tag="PLW15_03085"
     CDS             861..1496
                     /gene="hisH"
                     /locus_tag="PLW15_03085"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOT33416.1"
                     /translation="MISPSVVVLDYGSGNIHSAAKALELAGARVSLSSEKSVAEAADG
                     LLVPGVGAFSAVMAGINSVRGGAAIEKRLTGGRPVLGICVGMQVLFEHGVERGNDTPG
                     LGEWPGVVTELDAPILPHMGWNTVDAPADSRLFAGIADERFYFVHSYAAREWTLEVHD
                     PFPHPRLTWANHGGDTFLAAVENGPLSATQFHPEKSGEAGIRLLKNWLETL"
     gene            1493..2242
                     /gene="priA"
                     /locus_tag="PLW15_03090"
     CDS             1493..2242
                     /gene="priA"
                     /locus_tag="PLW15_03090"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HOT33417.1"
                     /translation="MSEFTSTPRLVLLPAVDVAGGKAVRLTQGEAGSETSYGDPVDAA
                     QEWIGQGAEWIHLVDLDAAFGRGNNHAVIKKVIKNTPRRVNIELSGGIRDDASLEAAL
                     ATGAKRINLGTAALENPEWAAHVIAEYGDAIAVGLDVRGTTLAARGWTQEGGDLWQVM
                     DRLELAGCARYVVTDVTKDGTLKGPNIDLLNQVMARTDRPVIASGGVATLDDIVELRE
                     LVPHGLEGAIVGKALYAKAFTLAQALDVASS"
     gene            2242..2991
                     /locus_tag="PLW15_03095"
     CDS             2242..2991
                     /locus_tag="PLW15_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HOT33418.1"
                     /translation="MSDSAGVPWEGRHFEPNPAADDDGSAPEPLMAAIRRFRAGEGGE
                     AEVVDAVRESRLLVALVAELGDTGVSPLGHTVDKSQELSIVTVAGPDGRSVLPAFSSV
                     TAMSAWNPKARPVPAAGTRVALAAASESTELVVLDPTSETEFAIRRPALWAIAQSVSW
                     IPSYLDPEVLRAFMDAAAPEPTVLAVTLAPGDPAARLAGPELVVRLALIEGLDRASLD
                     AVIARLQGRWAASEIITTRVDSLRVQLLSAV"
     gene            complement(2996..3346)
                     /locus_tag="PLW15_03100"
     CDS             complement(2996..3346)
                     /locus_tag="PLW15_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOT33419.1"
                     /translation="MADAPDHIDDEIRDISDVPAVELINTVAVHLMSAAAVKLGLGDT
                     PDAAADLDLDEARKLITSLAGLVTAAAPEISDNHARPLRDGLRSLQLAFREASFIQDA
                     PGAGPGEKYTGSVI"
     gene            3585..4406
                     /gene="infC"
                     /locus_tag="PLW15_03105"
     CDS             3585..4406
                     /gene="infC"
                     /locus_tag="PLW15_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT33420.1"
                     /translation="MHTNYEKETRISDPRTNDRIRVPEVRLVGPNGEQVGVVRIEDAL
                     RLAQEADLDLVEVAPNSKPPVAKIMDYGKFKYEEAQKAKEARRNQANTILKEVRFRLK
                     IDTHDYETKRKRAEGFLQAGDKVKAMILFRGREQSRPEQGVRLLQRFAEDVSEFGSVE
                     STPTIDGRNMVMVIGPHKTKAAAKAEAEARKAANNSKPARTDAPAAAAAPEAAPKVAA
                     PAAPAEAAAPEAAAPKAAAPKAAAPKAAAPKAAAPADVAAGKPAPVVPKKEEKDA"
     gene            4399..4593
                     /gene="rpmI"
                     /locus_tag="PLW15_03110"
     CDS             4399..4593
                     /gene="rpmI"
                     /locus_tag="PLW15_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT33421.1"
                     /translation="MPKQKTHSGAKKRFKLTGSGKVMKQQAGMRHNLEVKSGKTKRRL
                     NQEEVLAPQDAKVIKKLLGK"
     gene            4612..4998
                     /gene="rplT"
                     /locus_tag="PLW15_03115"
     CDS             4612..4998
                     /gene="rplT"
                     /locus_tag="PLW15_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT33422.1"
                     /translation="MARVKRAVNAHKKRRVILDRASGYRGQRSRLYRKAKEQVTHSLV
                     YAYRDRRARKGDFRRLWIQRINAASRANGLTYNRLIQGLNLAGIEVDRRILADLAVTE
                     PATFAAIVATAKAALPADTSAPKVNA"
     gene            5049..5849
                     /locus_tag="PLW15_03120"
     CDS             5049..5849
                     /locus_tag="PLW15_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT33423.1"
                     /translation="MIDNPRSPRVRAVAKLAKREARSQTGLFLLEGPQAVSEALTFRP
                     ELIVDLFATPTALERHPGIAAAAAETDLEVEFVTEQVLEAMADTVTPQGVVAVCRQFP
                     TSVKDILAGQPKLIAILEEVRDPGNAGTIIRAADAAGADAVIFTGRSVDLYNPKVVRA
                     TTGSLFHLPVAVDNELESVIERTKAVGVQVIAADIKGADLLAARKAGALASPTAWLFG
                     NEARGLSDEHYALADWVITVPIYGHAESMNLATAASVCLYESAFAQRS"
     gene            5934..6971
                     /gene="pheS"
                     /locus_tag="PLW15_03125"
     CDS             5934..6971
                     /gene="pheS"
                     /locus_tag="PLW15_03125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT33424.1"
                     /translation="MPDPTITDSSVDAARDDALAAIAASTTLTELRAARAAHAGEASP
                     LAQFNASLKTVAPDQRAAAGKLVGQARARVAEALSSREAELAVAEESARLASEAVDVT
                     AVATRWRSGARHPLSLLMEQMSDIFIGMGWEVAEGPELENEWFNFDALNMDEDHPARG
                     TQDTFFIDPIESHLLLRTQTSPVQVRSLLDRPLPVYVVSPGKVFRKDELDATHTPVFH
                     QLEGIAIDKGLTMAHLRGTLEHLARAMFGEGAEIRLRPNYFPFTEPSAEMDVWQPNAK
                     GGARWVEWGGCGMVNPNVLRAAGIDPEVYQGFAFGMGIERTLQFRNEMNDMRDMVEGD
                     VRFSQQFGMVV"
     gene            6973..9474
                     /gene="pheT"
                     /locus_tag="PLW15_03130"
     CDS             6973..9474
                     /gene="pheT"
                     /locus_tag="PLW15_03130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT33425.1"
                     /translation="MRIPISWLGEFVDLPEDVTHEHVHAALVKVGLEEEDVHGFELSG
                     PVVVGQVLEFTPEPQANGKTINWCQVDVGEAEPRGIVCGAHNFVVGDKVVVSLPGAVL
                     PGPFPISARKTYGHVSDGMIASARELGLGDEHDGILRLASLGLDPEVGTDAIALLGLD
                     DFAVEVNVTPDRGYAFSIRGIAREYANSTGAAFRDPISVVEPVETEASGFPVTIADDA
                     PIRGRVGATVFMSRAVRGVDATRPTPPWMVSRLALAGIRSVSLVVDITNYVMLETGQP
                     THAYDLDRLTGGITVRRAKAGETITTLDGKQRTLDPEDLLITDESGPIGIAGVMGGES
                     TEIDAATSTNVLVEAANFDPVTIARSARRHKLPSEASRRFERGVDPNAAAPAVARVVQ
                     LLVELAGGTADTVGSTIDESSAPAPIDLPDGYVEGLIGVEFSADEITGSLIAIGATVE
                     ASDTGWRVTPPTWRPDLTFPATLAEEVARIVGYDRIPSELPTAPPGRGLSREQRLRRS
                     VASSLASAGLTEVLAYPFIAPASNDLFGSAVAGPVAAMTVANPLDPDAAALRTSLLPG
                     LVTVAHRNLSRGLTDLALFEVGTVFLPEPGATYGSGPLPAGDARPSDEVLGALRASIP
                     PQPWHVGALFLGDAVAKQPGQPAVPTGLADALAAAHHIAELVAAPLTVVTGHHQAMHP
                     GRTAELRIGERPVGFAGELLPSLATELDLPRVVAVLELDLDALIAAGATQISATPISS
                     FPAATQDVSLVVAADVPAGDVLAVLREGAGELLEHAVLTDDYRGPGVAEGTKSLTYAL
                     RFRAPDRTLTQAEATEAKTAAVALAATTFGATLRE"
     gene            complement(9668..10213)
                     /locus_tag="PLW15_03135"
     CDS             complement(9668..10213)
                     /locus_tag="PLW15_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOT33426.1"
                     /translation="MSDLDGLINLIPIGDIAKKLGIDENVAKAAVAVAVPAIVGGLAA
                     NAKTDTGAQKLQAAVEHHDKKVSVDDVDVEDGQKIVKHVFGAKADDVAAAAGAKAGGP
                     DIGAIVAQVLPIIAPIVLAFLAQQMTKGGSSTAAAPKAEEPAAATGTDIGSILGGLLS
                     SKQGQDIIGGALGGLLGGGTK"
     gene            10481..11527
                     /gene="argC"
                     /locus_tag="PLW15_03140"
     CDS             10481..11527
                     /gene="argC"
                     /locus_tag="PLW15_03140"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT33427.1"
                     /translation="MSLSVAVAGASGYAGGEVLRLLSAHPDFSVTTVTAFQNAGQLLG
                     AVQLHLRSLAHLELQPTDAATLAGHDVVFLALPHGKSGEVTRDLPADTLVVDCGADHR
                     LTDPSAWETFYGGEFFGAWDYGLPELLLASGGTQRSALTGSKRIAVPGCNVTAITLAL
                     APGIRAGVIEPSDLVAVLAVGPSGAGKTLRTDLLASELLGSAHAYQVGGIHRHTPEII
                     QNLTVAGGVGVSVSFTPVLVPMSRGILATSTARLVPGTTATQVRDAWEQAYADEPFVH
                     LLPAGQFPRTADTVGANTALIGLAIDEAAGRVVVVSAIDNLVKGTAGAAIQSANIALG
                     LPETTGLTVDGVAP"
     gene            11524..12672
                     /gene="argJ"
                     /locus_tag="PLW15_03145"
     CDS             11524..12672
                     /gene="argJ"
                     /locus_tag="PLW15_03145"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOT33428.1"
                     /translation="MSVTAAKGFEAAGVTAGLKSSGGLDFALVVNRGPLANAAAVFTS
                     NRSKANPIIWSQQAIADGTASAIVLNSGGANCFTGAQGFQVTHSTAEAVAFALGVSSG
                     DVLVCSTGLIGDQLPLDKILAGVELAAAALGDHGEEASLAIMTTDSKPKRSIKQGNGY
                     TVGGMAKGAGMLAPGLATMLVVITTDVVLSSAELDIALRRATRVSFDRLDSDGCMSTN
                     DQVSLLASGASGVEPSLDSFTEVLTAVCLDLAEQLQGDAEGASHDIRIEVVGAATEDD
                     AVEVGRSVARNNLFKAAVFGNDPNWGRVLAAIGTTDAAFDPYDVDVSMNGVRVCHAGA
                     PDRPRTDVDLSPRRVDLLIDLKSGQHTATILTNDLTHDYVHENSAYSS"
     gene            12672..>13190
                     /gene="argB"
                     /locus_tag="PLW15_03150"
     CDS             12672..>13190
                     /gene="argB"
                     /locus_tag="PLW15_03150"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041464908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT33429.1"
                     /translation="MVSQDQAAAKAAVLIESLPWLKRFHDEIIVVKFGGNAMVDDDLK
                     RAFAEDMAYLRFAGVKPVVVHGGGPQITAELTRRGIPSEFKGGYRVTTPESMPVIRQV
                     LAEQVGGELVGLINEHSITEHAYLAEGISGDQYGLFTGEPRAAVVDGELVDLGLVGDV
                     TSVDPAAVLLEVA"
BASE COUNT         2066 a         4609 c         4436 g         2079 t
ORIGIN      
        1 gcaccagcgc gaccgcatga ttctcgccct gcccgccctc ggctacacgc cgctgcccag
       61 cgcggccaat ttcgtgctct tcggtggtgt ggccgatccg cacgccgtct tcgaggctct
      121 tcttgagcgc gacatcatca tccgcgaggt gggcatccct caccacctgc gggtcaccgc
      181 gggcagtgat gccgagacga ccctcttcct cgacgcgctc gggcagctcg agtcgatcgg
      241 taggatggcg ccatgaccgc aggccgcacc gcgagcatca gccgcaccac gagcgagtcg
      301 agcatcgaac tctcgctcga cctcgacggc acgggcacat cgtcgatctc gacgagcgtg
      361 ccgttcttcg accacctgct gacggccttc tccaagcact cgctcgtcga cctcaccgtc
      421 acggccaccg gcgacacgca catcgatgtt caccacaccg tcgaagacgt cggcatcgca
      481 ctgggcacag ccatccgcca ggcgctcggc gacaaggcgg gcatctcgcg gttcggcgac
      541 gcgctggttc ctctcgacga ggcgctcgtg caggccgtcg tggatgtctc gggccgcccc
      601 tacctcgtgc acaccggcga gccggccggc ttcgagcacc acctgatcgg tggccacttc
      661 acgggctcga tgctgcgtca cgtgttcgag gccatcacct tcaacgcggg actgaccgtg
      721 cacatcaccg tgctcggcgg gcgcgatccc caccacatcg ccgaagccga gttcaaggcg
      781 ttcgctcgcg cgttccgact cgcggtgcag cccgatgccc gtgtcagcgg catcccgtcg
      841 accaagggtg ccctctagcc gtgatctctc cctccgtcgt cgttctcgac tacggctccg
      901 gcaacatcca ctcggccgcc aaggcgctcg agctggccgg agcccgagtg tcgctgtcga
      961 gcgagaagag cgtggccgag gccgccgacg gcctgctcgt tccgggcgtc ggcgcattct
     1021 cggccgtcat ggccggcatc aactcggtgc gcgggggagc cgcgatcgag aagcggctca
     1081 ccggcgggcg accggttctc ggcatctgcg tcggcatgca ggtgctgttc gagcacggcg
     1141 tcgagcgggg caacgacacc cccggcctcg gtgagtggcc cggcgtcgtg accgagctgg
     1201 atgccccgat cctgccccac atggggtgga acacggtcga cgctcccgcc gattcgcgcc
     1261 tgttcgccgg catcgccgac gagcgtttct acttcgtgca ctcctacgcc gcccgtgagt
     1321 ggacgctcga ggtgcacgac ccgttcccgc accctaggct cacctgggcg aatcacggcg
     1381 gcgatacctt cctcgccgcg gtggagaacg gccccctctc ggcgacgcag ttccatcccg
     1441 agaagtcggg cgaagccggc atccgcctcc tgaagaattg gttggaaacc ctatgagcga
     1501 gttcaccagc acaccccgac tggtgttgct tcccgctgtc gacgttgccg gcggcaaggc
     1561 cgtgcggctg acgcagggcg aggcgggctc ggagaccagc tacggcgatc ccgtcgacgc
     1621 ggcgcaggag tggatcggcc agggcgccga gtggattcac ctggtcgacc tcgacgcggc
     1681 attcgggcgc ggcaacaacc acgccgtcat caagaaggtc atcaagaaca ccccgcggcg
     1741 ggtcaacatc gagctgtcgg gtggaattcg ggatgacgcg agcctcgagg ccgcactcgc
     1801 gaccggggcc aagcgcatca acctcggcac cgccgcactc gagaacccgg agtgggcggc
     1861 ccacgtgatc gcggagtacg gcgacgcgat cgccgtcggt ctcgatgtgc gcggcaccac
     1921 gctggccgcc cgcggctgga cgcaggaggg cggtgacctc tggcaggtca tggaccgcct
     1981 cgaactcgcc ggctgcgcac gctacgtcgt caccgatgtc accaaggacg gcaccctcaa
     2041 gggccccaac atcgacctgc tcaatcaggt gatggcccgc accgaccggc cggtgatcgc
     2101 ctccggggga gtcgccactc tcgacgacat cgtcgaactg cgtgaactgg ttccccacgg
     2161 actcgagggc gcgatcgtgg gcaaggcgct gtacgccaag gccttcaccc tcgctcaggc
     2221 tctcgacgtc gccagcagct agtgtccgac tcggccggcg tgccctggga gggtcgccac
     2281 ttcgaaccga accccgccgc tgatgacgac ggttcggcgc ccgagccgct catggccgcg
     2341 atccgtcgat tccgggcagg cgaggggggc gaggccgagg tcgtcgatgc cgtgcgcgag
     2401 tcgcgcctgc tggtcgcgct cgtcgcggag ctcggcgaca ccggcgtgag ccctctgggg
     2461 cacaccgtcg acaagtctca ggagctctcg atcgtcacgg tcgccggccc cgacggccgc
     2521 agcgtgctcc ctgccttcag ttcggtgacg gcgatgagcg cctggaaccc gaaggcccgg
     2581 cccgttccgg cggcaggtac gcgtgtggcg ctggccgccg cgagcgagtc gaccgaactc
     2641 gtcgtgctcg accccaccag cgagacggag ttcgccattc gtcggcccgc gctctgggcg
     2701 atcgcccagt ccgtgtcgtg gatcccgagc tatctcgacc ccgaggtgct ccgtgccttc
     2761 atggatgccg cggcccccga accgacggtg ctcgccgtga cgctggcccc cggtgaccct
     2821 gccgcccgtc tcgcgggtcc ggaactcgtc gtgcggctcg cgctcatcga ggggctggat
     2881 cgtgcgtccc tcgatgccgt catcgcgcgc ctgcaggggc ggtgggcggc atcggagatc
     2941 atcaccacgc gggtcgactc cctgcgggtg cagttgctga gcgcggtcta gcggctcaga
     3001 tcacgctgcc ggtgtacttc tcgccggggc cggcacccgg cgcatcctga atgaacgacg
     3061 cctcgcggaa cgcgagctgc agcgagcgca gcccgtcgcg cagcgggcgc gcgtggttgt
     3121 ccgagatctc gggtgctgcc gcggtcacga ggccggcgag cgaggtgatg agcttgcggg
     3181 cctcgtcgag atcgaggtcg gccgccgcat ccggggtgtc accgaggccg agcttgaccg
     3241 cggcggcgct catcaggtga accgcaaccg tgttgatgag ctcgaccgcc ggaacgtcgg
     3301 agatgtcgcg aatctcatcg tcgatgtggt cgggggcgtc ggccatggag catccttagt
     3361 gaatcggggc ggcgtctgct attcttgtgc aggctccggg gctcgccccg gctacgaaag
     3421 tggagattct cccacctgtc aaccgcttct caaggttacc gggtagttac accccgccag
     3481 gccgcgtcac tgcgtgcctg gtagcagcaa gggtgttggc gcgacagcgc tcgtgaaaat
     3541 cctctctcgt tctgcctgcg gcagccgccg cactgcaccg ttcggtgcac acgaactacg
     3601 agaaggagac ccgcatcagc gatccccgta ccaatgaccg aatccgtgtt ccggaggtcc
     3661 gtcttgttgg ccccaatggc gagcaggtgg gcgtcgtccg catcgaggac gcactccgtc
     3721 tggctcagga ggccgacctg gatttggttg aggtagctcc caattccaag cctcccgtcg
     3781 ccaagatcat ggattacggc aagttcaagt acgaagaggc ccagaaggcc aaggaagcgc
     3841 ggcgcaacca ggcgaacacg atcctcaagg aggttcgttt ccgcctcaag atcgacaccc
     3901 acgactacga aacgaagcgc aagcgcgccg agggattcct ccaggctggc gacaaggtca
     3961 aagcgatgat cctgttccgc ggtcgcgagc agtcccgtcc cgagcagggc gtgcgcttgc
     4021 tgcagaggtt cgccgaggat gtgtcggagt tcggttcggt cgaatcgacc ccgaccatcg
     4081 acggtcgcaa catggtcatg gtgatcggcc cgcacaagac caaggcggcg gcgaaggccg
     4141 aagccgaggc tcgcaaggcc gccaataact ccaaaccagc ccgaacggat gcccctgctg
     4201 ccgcagcggc gcccgaggcg gcaccgaagg ttgcagcccc agcggcacca gccgaggcag
     4261 cggccccgga ggcagcagca ccgaaggccg cggcgcccaa agccgccgcc cccaaggctg
     4321 cagcgccgaa ggccgcagca ccagcagacg tggcggcggg aaaacccgcc ccagtagttc
     4381 cgaagaaaga ggaaaaggat gcctaagcag aagacgcact ccggtgccaa gaagcgcttc
     4441 aagctcaccg gtagcggcaa ggtcatgaag cagcaggctg gcatgcgcca caacctggag
     4501 gtcaagagcg gcaagacgaa gcgccgcctg aaccaggaag aggtcctcgc tccgcaggac
     4561 gccaaggtca tcaagaagct tctcggcaag taagggtcag gtacagaaga aatggcaaga
     4621 gtaaagcggg cggtcaacgc ccacaagaag cgtcgcgtca tcctcgatcg cgcctccggt
     4681 taccgcggcc agcgttcgcg cctgtaccgc aaggcgaagg agcaggtcac ccactcgctc
     4741 gtctacgcgt accgtgaccg ccgtgcccgc aagggtgact tccgtcgcct gtggatccag
     4801 cgcatcaacg ccgcgtcgcg tgcgaacggc ctgacctaca accgcctcat ccagggtctc
     4861 aacctggccg gcatcgaggt cgaccgtcgc atcctcgctg acctcgcggt caccgagccc
     4921 gcgacgttcg ccgccatcgt cgccaccgcg aaggccgcac tgccggccga cacgagcgcg
     4981 ccgaaggtga acgcataggt tcagcgcggc gaaagccagc gttcagttag ccgcaatagg
     5041 ctgtcgacat gatcgacaac ccgcgctcac ctcgggtgcg cgctgtggca aaactcgcta
     5101 agagagaagc ccggtcgcag accgggcttt tcctcttgga gggaccccaa gccgtgtcgg
     5161 aggctctgac cttccggccc gagctcattg tcgacctctt cgcgactccc acggcgctgg
     5221 agcgccaccc cggtattgct gctgccgcag ccgagaccga cctcgaggtc gagttcgtca
     5281 ctgagcaggt gctcgaggca atggccgaca cagtaactcc acagggggtt gtcgccgtct
     5341 gccgtcagtt ccccacctcg gtgaaggaca tcctggccgg gcagccgaag ctcatcgcga
     5401 tcctcgagga ggtgcgcgac ccgggcaacg ccggcacgat catccgtgcc gcggatgccg
     5461 ccggcgccga cgcggtcatc ttcaccggtc gcagcgtcga cctctacaac ccgaaggtgg
     5521 ttcgcgcgac caccgggtcg ctgttccacc ttcctgtcgc cgtcgacaac gagctggaga
     5581 gcgtgatcga gcgcaccaag gcggtcggcg tgcaggtgat cgccgccgac atcaagggcg
     5641 ccgaccttct cgctgcgcgc aaggcggggg cgttggccag tcccacggca tggctgttcg
     5701 gcaacgaggc gcgcggcctg agcgacgagc actacgcgct cgccgactgg gtgatcaccg
     5761 tgccgatcta cggccacgcc gaatcgatga acctcgcgac cgcggcatcc gtctgcctct
     5821 acgagagtgc gttcgctcag cgcagctgag cgaaccatct gcgcagcgct cagcgcagct
     5881 gagcgaacgc actgcgcagc cgcgcagcgc agctgacagt aaactcgtca cttgtgccag
     5941 accccacgat caccgacagc agcgttgacg ccgcgaggga tgacgccctc gccgccatcg
     6001 ccgcctccac gaccctgacc gagctgcgcg ccgcccgcgc ggcgcacgcg ggggaggcct
     6061 ctccgctcgc ccagttcaac gcgagcctga agacggtcgc ccccgatcag cgtgccgcag
     6121 caggaaagct cgtcggccag gcccgggctc gtgttgccga ggcgctgtcg tcgcgcgagg
     6181 cggagcttgc agtggccgag gagtccgcgc ggctggcgag cgaggcggtg gatgtcacgg
     6241 cggtggccac gcgctggcgc tccggtgcgc ggcatccgct cagcctgctc atggagcaga
     6301 tgtcggacat cttcatcggc atgggctggg aggtcgcgga gggtcccgag ctcgagaacg
     6361 agtggttcaa cttcgacgcg ctcaacatgg acgaggatca ccccgcccgc ggcacgcagg
     6421 acacattctt catcgacccg atcgagtcgc acctgctgct gcgcacgcag accagcccgg
     6481 tgcaggtgcg gtcgctgctc gatcggccgc tgccggtcta cgtggtgtcg cccggcaagg
     6541 tgttccgcaa ggacgagctc gacgcgaccc acacgccggt cttccaccag cttgagggca
     6601 tcgccatcga caagggcctg acgatggcac acctgcgcgg caccctcgag cacctcgcgc
     6661 gtgcgatgtt cggtgagggc gccgagattc gcctgcgccc caactacttc ccgttcaccg
     6721 agccgagcgc cgagatggac gtctggcagc cgaacgccaa gggcggcgcc cgctgggtcg
     6781 agtggggcgg ctgcggcatg gtcaacccca acgttctgcg cgccgcgggc atcgatcccg
     6841 aggtctatca gggcttcgcg ttcggcatgg gcatcgagcg caccctgcag ttccgcaacg
     6901 agatgaacga catgcgcgac atggtcgagg gcgatgtgcg cttctcgcag cagttcggaa
     6961 tggtggtctg agatgcgcat ccccatctcc tggctcggcg aattcgtcga cctccccgag
     7021 gacgtcaccc acgagcacgt gcacgcagcg ctcgtcaagg tcgggctcga agaggaggac
     7081 gtgcacggct tcgagctgtc gggtcccgtc gtcgtcgggc aggtgctcga gttcactccc
     7141 gagccgcagg ccaacggcaa gacgatcaac tggtgccagg tggatgtcgg cgaagccgag
     7201 ccgcgcggca tcgtctgcgg cgcccacaac ttcgtggtcg gtgacaaggt cgtggtctcg
     7261 ctgccgggcg ccgtgctgcc gggtcccttc ccgatctccg cgcgcaagac ctacggccac
     7321 gtctccgacg gcatgatcgc ctcggctcgc gagctgggtc tcggcgacga gcacgacggc
     7381 atccttcgtc ttgcgagcct cgggctcgac cccgaggtgg gcacggatgc gatcgccctc
     7441 ctcggcctgg acgacttcgc cgtcgaggtc aacgtcacgc ccgaccgcgg gtacgccttc
     7501 tcgatccgag gcatcgcacg ggagtacgcc aactccacgg gggcggcgtt ccgcgacccc
     7561 atctcggtgg tcgagccggt cgagaccgag gcgtcgggct tcccggtgac catcgccgac
     7621 gacgcgccga tccgcggccg cgtcggcgcg acagtgttca tgtcccgcgc cgttcgcggc
     7681 gtggatgcaa cccggcccac cccgccgtgg atggtgtcca ggctggccct cgcgggcatc
     7741 cgctcggtgt ctctcgtggt cgacatcacc aactacgtga tgctcgagac cggacagccc
     7801 acccacgcct acgacctcga tcggctgacc ggtggcatca ccgtgcgccg cgcgaaggcg
     7861 ggggagacga tcacgaccct cgacggcaag cagcgcaccc tcgaccccga ggacctgctg
     7921 atcaccgacg agtccggccc gatcggtatc gcgggcgtca tgggcggcga gtccaccgag
     7981 atcgacgctg caacctcgac gaacgtgctg gtcgaggccg cgaacttcga ccccgtgacc
     8041 atcgcgcgca gcgcccgacg ccacaagctt ccgagcgagg catcccgtcg cttcgagcgc
     8101 ggtgtcgacc ccaacgcggc ggcaccggcc gtcgcgcgag tggtccagct gctcgttgag
     8161 cttgccggcg gtaccgctga caccgtgggc tcgactatcg acgagtcctc ggcgccggca
     8221 cccatcgatc tgccggacgg ctacgtcgag ggcctcatcg gtgtcgagtt ctccgccgac
     8281 gagatcaccg gctcgctgat cgcgatcgga gcgacggtcg aggcctccga caccggatgg
     8341 cgcgtgacgc cgcccacgtg gcgccccgac ctgaccttcc ccgcgacgct cgctgaggag
     8401 gtcgcccgca tcgtcgggta cgaccgcatt ccgtctgagc tgcccaccgc accgcccggg
     8461 cggggtctca gccgcgaaca gcgtctgcgc cgttcggtcg cgagttcgct cgcgtccgcc
     8521 ggcctcacgg aggtgctggc gtacccgttc atcgctccgg cctccaacga cctcttcggt
     8581 tcggcggtcg caggcccggt cgccgcgatg actgtcgcca acccgcttga tccggatgcc
     8641 gcggcgctac gcacctcgct gctgccgggt cttgtcactg tcgcccaccg caacctgtcg
     8701 cgcggcctga ctgacctcgc gctgttcgag gtgggcaccg tgttcctgcc ggagcccggc
     8761 gcgacctacg gcagcggtcc gcttcctgcg ggtgacgcgc gcccctccga cgaggtgctc
     8821 ggcgcgctcc gcgcgagcat cccgccccag ccgtggcacg tcggcgcgct gttcctcggc
     8881 gatgccgtgg ccaagcagcc cggccagccg gcggtgccga ccgggctcgc ggatgccctc
     8941 gccgccgcac atcacattgc cgagctcgtc gcggcgccgc tgaccgtggt gacgggtcac
     9001 caccaggcca tgcaccccgg ccgcacggcc gagctgcgga tcggtgagcg tcccgtgggc
     9061 ttcgctggcg aactgctgcc gtcgctggcc accgagctgg atctgccgcg cgtggttgct
     9121 gtgctcgagc tcgacctgga tgcgctcatc gccgcggggg cgacgcagat ctcggcgacc
     9181 ccgatctcgt ccttcccggc cgcgacgcag gatgtctcgc tcgtcgttgc ggcggacgtg
     9241 ccggccggcg acgtgctcgc cgtgctgcgc gagggcgcgg gcgagctgct cgagcacgcg
     9301 gtgctcaccg acgactaccg cggtcccggc gtggccgagg gaacgaagtc gctcacctac
     9361 gcgctgcgct tccgagcccc cgaccgcacc ctcacgcagg cggaggcgac ggaggcgaag
     9421 accgcagcag tcgccctggc cgccaccacc ttcggggcaa ccctccgcga gtaaccgcgc
     9481 tccctcctcc cgtgctgagg gggagccgag gtgggctgct cggaggcggg gcgattgatg
     9541 gttcagcgag cgcaaggggt gttgcgtttc ttgcgagtta tgagaaagtc ccgacggccg
     9601 gatggccgtc gggactttct cattgtctga gctgaaacgc agcgccgctt gcgcgaagca
     9661 ccccgagtta cttggtgccg cctccgagca gcccgccgag cgcgccaccg atgatgtctt
     9721 ggccctgctt agacgagagc aggccgccga ggatgctgcc gatgtcggtg ccggtcgcgg
     9781 ccgcgggctc ttctgccttg ggcgctgccg cggtgctgct gccgcccttg gtcatctgct
     9841 gggcgaggaa cgcgagcacg atgggggcga tgatcgggag cacctgcgca acgatggcgc
     9901 cgatgtcggg accgcccgcc ttggctccgg cggcggcggc gacgtcgtcg gccttggcgc
     9961 cgaacacgtg cttgacgatc ttctggccgt cttcgacgtc gacgtcgtcg acgctcacct
    10021 tcttgtcgtg gtgctcgacg gcggcctgga gcttctgggc gccggtgtcg gtcttggcgt
    10081 tggccgcgag gcctccgacg atggcgggca cggcgaccgc gacggcggcc ttggcgacgt
    10141 tctcgtcgat tccgagcttc ttcgcgatgt cgccgatggg gatgaggttg atgaggccat
    10201 cgaggtcgga catgggttct cctgtgttct ggtggtgctc ctggtgagca tcgatcatca
    10261 gcgtagcgat tcgcgctgtc caacgggatg ggggtacgct ctgtcacgtg accggcttca
    10321 cccgcaccct cgagcgcttc gcgccgaccg ttgtgctgcg ccgacgtcgc gacgagcgac
    10381 gctaggcggt gtctcgtctg cgaccgggag tgatcttctc tgcggtcgcg tgagtccccg
    10441 ccgcgtcatc ccctcgtccg tctctcaata aggtagagcc atgtccttgt cggttgccgt
    10501 cgctggtgcc agcggctacg ccgggggtga ggtgctgaga ctgctctcgg cgcaccccga
    10561 tttctctgtc accaccgtga ccgcgttcca gaacgccggc cagttgctgg gtgccgttca
    10621 gctgcacctg cggtcgctcg cccatcttga gttgcagccg acggatgccg cgactctcgc
    10681 tggccatgac gtcgtgttcc tggcgctgcc gcacggtaag tcgggtgagg tcacccgtga
    10741 tctgccggcc gacacgctgg tcgtggactg cggagccgat caccgtctca ccgacccctc
    10801 ggcgtgggag acgttctacg gcggcgagtt cttcggcgcc tgggactacg gcctgcccga
    10861 gttgctcctc gcgtccggcg gcactcagcg cagcgcgctc acgggcagca agcgcatcgc
    10921 ggttccggga tgcaacgtca cggccatcac gctggccctc gccccgggca tccgggccgg
    10981 cgtcatcgag ccgagcgacc tcgtcgcggt gctcgcggtc ggaccgtcgg gtgcgggcaa
    11041 gactttgcgc accgacctgc tggccagcga actgctgggc tccgcgcacg cgtaccaggt
    11101 gggcggcatc caccggcaca cgccggagat catccagaac ctgacggtcg ctggcggcgt
    11161 cggcgtgagc gtgtcgttca ctcccgtgct ggtgccgatg agccggggca tcctcgcgac
    11221 gtcgacggca cgcctcgtgc ccggcaccac cgcaacacag gtgcgggatg cctgggagca
    11281 ggcctacgct gacgagccgt tcgtgcacct gctgccggcc ggccagttcc cgcgcacggc
    11341 cgacacggtg ggcgccaata ccgcgctgat cgggctcgcg atcgacgagg cggcaggacg
    11401 cgtggtcgtg gtgagcgcga tcgacaacct ggtgaagggc accgcggggg cggcgatcca
    11461 gtcggcgaac atcgccctgg ggctgccgga gacgaccgga ctgactgtgg atggggtggc
    11521 accgtgagtg tgacagcggc gaagggtttc gaggcggccg gcgtgacagc gggtctcaag
    11581 tcgagcgggg ggctggactt cgccctcgtg gtgaaccggg gtccgctggc caacgctgcg
    11641 gccgtgttca cgagcaaccg gtcgaaggcc aacccgatca tctggtcgca gcaggccatc
    11701 gccgatggca cggcgagcgc gatcgtgctc aactccgggg gagcgaactg cttcaccggt
    11761 gcgcagggtt tccaggtgac gcactccacg gcggaggcgg tggcattcgc actcggcgtc
    11821 agctcgggtg acgtgctcgt ctgctcgacc ggtttgatcg gcgaccagct tcccctcgac
    11881 aagatcctcg cgggcgtcga actcgcggcc gccgctctcg gcgaccacgg cgaggaggcg
    11941 agcctggcga tcatgaccac cgactcgaag ccgaagcgct cgatcaagca gggcaacggc
    12001 tacacggtcg gcggcatggc gaagggcgcg ggcatgctcg cgccgggcct cgcgacgatg
    12061 ctcgtggtca tcaccaccga cgtcgtgctc tcgagcgccg aactcgacat cgcgctgcgc
    12121 cgggccaccc gcgtgagttt cgaccgcctc gactccgacg gctgcatgtc caccaacgac
    12181 caggtgtcgc tgctggcgag cggggcgtcc ggcgtggagc ccagcctgga ttcattcacc
    12241 gaagttctga ccgccgtgtg tctcgacctc gccgagcagc tgcagggcga cgcggaagga
    12301 gccagccacg acatccgcat cgaggtcgtc ggcgcggcaa ccgaggacga cgctgtcgag
    12361 gtcggtcgct ccgtcgcccg caacaacctg ttcaaggccg cggtcttcgg caacgacccc
    12421 aactggggcc gggtgctcgc cgccatcgga accacggatg ccgccttcga cccgtacgac
    12481 gtcgacgtga gcatgaacgg ggtgcgggta tgccacgccg gggcacccga ccggccgcgt
    12541 accgacgtcg acctgtcacc gcgccgcgtg gacctgctca tcgacctcaa gtcgggccag
    12601 cacacggcga cgatcctcac gaacgatctc acgcacgact acgtgcacga gaactcggcg
    12661 tactccagct gatggtcagc caggatcagg cggccgcgaa ggccgcagtg ctcatcgaat
    12721 cactgccgtg gctgaagcgc ttccacgacg agatcatcgt ggtcaagttc ggcggcaacg
    12781 cgatggtcga cgacgacctc aagcgcgcgt tcgccgagga catggcgtac ctgcggttcg
    12841 ccggggtgaa gcccgttgta gtgcacggcg gcgggccgca gatcaccgcc gagttgacgc
    12901 gccgcggcat cccgagcgaa ttcaagggcg gctaccgggt cactacccct gagtcgatgc
    12961 cggtgatcag acaggtgctc gccgaacagg tcggcggcga gctggtcggc ctgatcaatg
    13021 agcacagcat caccgagcac gcctacctcg ccgagggcat ctcgggcgac cagtacggac
    13081 tgttcaccgg cgaaccgcgg gcggccgtcg tcgacggcga acttgtggac ctcggtctcg
    13141 tcggcgatgt gacgtcggtc gatccggcgg cggtgctgct ggaggtcgcc
//
LOCUS       DAOSOU010000033        12939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4833_length_12939_cov_7.009314, whole genome shotgun sequence.
ACCESSION   DAOSOU010000033 DAOSOU010000000
VERSION     DAOSOU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12939
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4833_length_12939_cov_7.009314"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="otsB"
                     /locus_tag="PLW15_03155"
     CDS             complement(<1..693)
                     /gene="otsB"
                     /locus_tag="PLW15_03155"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOT33430.1"
                     /translation="MVDVDTPPRYSRLPEPLVGALRELARTKRLLVALDFDGTLAPEV
                     DEPEMARALPESRDAVLRLLAMPNTRVALVSGRALASLESVADLPDTVLLVGSHGIEI
                     RLDSPVAQVSLDTAELKQVDMINTVLGQVADSVDDVWLEAKPAGFALHTRLATEANSR
                     IAHLVARNEVKAEVNDVYIREGKDVLEFSARSTTKGEAVEHLRQYTQADAVFYAGDDV
                     TDEDAFAALSADD"
     gene            complement(697..2202)
                     /locus_tag="PLW15_03160"
     CDS             complement(697..2202)
                     /locus_tag="PLW15_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOT33431.1"
                     /translation="MPSATHAAPLEYDFVVVSNRLPVDRVVDADGIDAWAPSPGGLVT
                     ALEPVMRDADGAWVGWAGQPDLELDPFESGGIHLVPIALSADEIENYYEGFSNDTIWP
                     LYHDVIESPGYHRVWWESYVAVNQRFADATAAVSAQGATVWVQDYQLQLVPRMLRTAR
                     PDLTIGFFNHIPFPPYGIFSQLPWRTQVIEGLLGADVIGFQRVADASNFSRAVRRLTG
                     NPTRGPAIEVSLGTEPTRRVIAKSFPISIDARSFEALAREPRIQARAKEIREGLGNPR
                     TVMLGVDRLDYTKGIAHRMKAFGELLAEGRLNVEDVTLVQVASPSRERVEAYMNLRDE
                     IELTVGRINGDYGTMSHQAISYHHHGYPRDEMVALYLAADVMLVTALRDGMNLVAKEY
                     VASRFDEDGVLILSEFTGASDELKRALLINPHDIDGLKDAILAAIAMPRRERASRMRT
                     LRRRVHEFDVARWSADFLTTLDQAKRGRFQRMSPTELVDELEWTLDSRKAH"
     gene            2319..3140
                     /locus_tag="PLW15_03165"
     CDS             2319..3140
                     /locus_tag="PLW15_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT33432.1"
                     /translation="MISIGMSTSCVYPLPTESAFRLARDAGFDGIEVMVTNDPVTQDA
                     AALRYLSKTYGLPILSIHAPVLLLTQLVWGTNPVRKLERSAELAATLGASTVVVHPPF
                     RWQAGYARRFEATIRELAGVHSVEFAVENMFPLTLGKVGVRAYSPSSDPVFIDCDAIT
                     LDFSHAALGGRDSLEFAMAMGDRLRHVHLTDGTVSMRDGQLLDEHLVPGRGTQPVAEV
                     VRYLASTGWSGSLVAEVNTAGAGSEDGRLAMLRETREFAASQIAAGRRGRRSGVS"
     gene            3177..4193
                     /locus_tag="PLW15_03170"
     CDS             3177..4193
                     /locus_tag="PLW15_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HOT33433.1"
                     /translation="MPIVDNAIYREGVRQTRAVTLDQTFEALRDGGGFAWIGMYRPTE
                     DELRAVANEFGLHELAVEDALVGHQRPKLERYGETLFLVLRPARYLDAPEEVEFGEVH
                     LFIGPNFAITVRHAESPDLGRVRKRLEQNPALLALGPQAILYAVLDQVVDEYQPVIAG
                     LENDIDEIEDQLFQGDPAVSRRIYELATEAMGFQRATHPLVEMLGQLESGYEKYHVDM
                     ELQRDFRDVLDHTLQIVARADSFRAILQNALTVQSTLVTQAQNDEIRNMTQASIDQGD
                     QVKKISGWAAILFAPTLIGAIYGMNFRFMPELDWPLGYPFALGLMAVSGVVLYVVFKR
                     RGWL"
     gene            4245..4571
                     /locus_tag="PLW15_03175"
     CDS             4245..4571
                     /locus_tag="PLW15_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33434.1"
                     /translation="MERFSDDEKQAVFELMSERLFELFGPGGSFRITLGRASADDAVF
                     VSTVSDTIAWQVAASLVLEPAAAGHHAAAQPEHVERDDLWRHIEAELLAQRQAAESVS
                     VRVHAA"
     gene            4856..5830
                     /locus_tag="PLW15_03180"
     CDS             4856..5830
                     /locus_tag="PLW15_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT33435.1"
                     /translation="MTSTGLELRPGLHRVQAPLGERFVALYLLVGPKATLLVDTGIRE
                     SITENLLPYLAATGLGPITWAINTHCDFDHTGGNGALKAASPETQLLVHELDRRLTED
                     LQALIDVRYGEFRDADGFDDAPETTEYLRSVSDLVAVDRTVVGGEVLDLGDREVTILH
                     VPGHSPGHIAVHDPLNQALIIGDATLGATVPTADNRPAFPPTYRDVDDYVASAELFRS
                     LNPDLILTAHYPVYEGDATTAFLDETVEYTRLVDRVLADELRGRTAGATTLELIRSRG
                     AELGPWGAAALDYAIFPVTGNLERLEARGEVSSELIDGRRHWIWTGTR"
     gene            5827..6879
                     /locus_tag="PLW15_03185"
     CDS             5827..6879
                     /locus_tag="PLW15_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT33436.1"
                     /translation="MTVRLGAVGAGWWATSNHFPIFAARDDVELVGVCGLGPELDTVR
                     DQFGFGFSTGDYEQLLDADLDAVVITTPHDLHYEQAVRAIDRGLHVLVEKPVTLKADT
                     AWDLQRRTAAAGVHFVVPYGWNYKPFTERAKALIDAGHLGELQYVQCHMASPTRGLFA
                     GDPNDIRALWDSETSGPSVSTWQSPEHGGGYAHGQITHSSALMFWLSGLRAATVAGRT
                     SLGGARVDLFDSAVLTFDGGALGTLSGAATLPDINPYQVDVKLFGTEGVLLLDTERER
                     VSLHRYDGQAWDLDIEPGSGAYECIEPPNRFIDLITGASTVNNSDASIAARSVELIDA
                     LLRSSAAGGVEVAVHP"
     gene            6917..8161
                     /locus_tag="PLW15_03190"
     CDS             6917..8161
                     /locus_tag="PLW15_03190"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOT33437.1"
                     /translation="MTEPAATPGVLSGYRVLDCSIAMAGPFAAQRLGDLGADVIKVEP
                     TTGEWQRHTAAGGATGNKINVSFLSLNRNKRSVALDLKSADGKDALRELVRTADVFLQ
                     NYRPGVAARLGVDYESLRAINPRIIYVSISGYGESGPYRDRPGQDLLVQAMSGAMLSA
                     GRAGDEPSPAGQYLADAITASTAFEGVLAALLHRERTGEGQLVTVNMLDAVTTLQMQE
                     LSVFTVGGKPQERSAEAIAHVYIRAPYGVFRTSDGFVALAFADLHLLGELIDEPAFEG
                     WNSEVEGWTKRDEIHAKTAAKLVANTSQHWIDLLGGAGIWIGPVYGYADLVDDPQIAH
                     NGTFVEYDHPTEGHVKTPGFPYTFTKTPPQVYRGAPLVGEHTREILAEAGFDPERVDA
                     LLESGAAATTTASDPAPVSSRG"
     gene            8154..9227
                     /locus_tag="PLW15_03195"
     CDS             8154..9227
                     /locus_tag="PLW15_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33438.1"
                     /translation="MAEFTGLTWDHPRGRQALERSAASGTLIEWHVHPLEGFESSPID
                     ELAARYDVIVLDHPHLGDALATGALQSLDTVFEPDWLATLTCVGPSIRSYELDGHLWA
                     LPLDAATQVSARLPERVPENPATWTEVVELSHHQPVALSLAGPHAYLSFASLTVALGG
                     DPTDPGDTGLEALDLLRDLQSRAPAGTESLNPIGMLERTHTARDIAYIPLVYGYVPYA
                     QGPDPIHFGDAPTAVRSGSTIGGTGLALSSRCTPTPALLEYLRWLLHPVTQATYIPDN
                     AGQPAVRSAWLSPSVNAASSEFYLATLATIDAAWVRPRIAGFTPFQSEASRVLREAIL
                     GERSPASALAETAERFAALGVRA"
     gene            9224..10009
                     /locus_tag="PLW15_03200"
     CDS             9224..10009
                     /locus_tag="PLW15_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT33439.1"
                     /translation="MTITSTSEQVRVDTDAHVLTITLNRPEKLNSVTQSMSDALVAII
                     EEANADDEVRVIILTGSGQRSFCAGSDIASLDAYATPWEFRNRTDYCDALRSSRKPII
                     AAINGYAFGGGLETSLTCDIRLASATASFAAPEIKLGWIGGGGMATFLAHSIGASNAA
                     VMLMTGDPVDAQRALAWGLVSEVLEPDALLPRARELAATIASRPPIAAETAKSNLRAA
                     FNMPQDEAIRYERDLQTVTFATEDAAEGRAAFAEKRPGVFRRR"
     gene            10020..11186
                     /locus_tag="PLW15_03205"
     CDS             10020..11186
                     /locus_tag="PLW15_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT33440.1"
                     /translation="MTTALLPDDGTAGTLIGRVWDPRVSGPSVVAVRSTGVEDLSGSF
                     ATIRDVLENGDPAGAVAAASGPVLGSVADLLANTAIAGRDRTLPWLLSPIDLQVVKAA
                     GVTFPVSMLERIIEERARGEFDAAASIREEILTALGGSLRDLVPGSAEAMRVKELLIE
                     RGLWSQYLEVGIGPDAEVFTKAPVLSTVGTGVDVGIYSASKWNNPEPELVLAVASTGR
                     IVGATLGNDVNLRDIEGRSALLLGKAKDNTASAAVGPFIRLFDATYSMDDVRTAMVTL
                     EIDGADDGFRLAASSPLSEISRDPVDIVAQVIGDHHQYPDGFVLYLGTLFSPVDDRDV
                     VGHGFTHKIGDVVRIAEPRLGTLTNTVQFSENAEPWTFGIDALYRNLAARGLLK"
     gene            11196..12683
                     /locus_tag="PLW15_03210"
     CDS             11196..12683
                     /locus_tag="PLW15_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT33441.1"
                     /translation="MLTTTNPATGETTDSGIAVTADVAAVVDRAQHAFTALAAEPRAW
                     RADLLDALAAALEEHRDDLVALADSETGLGVARLTGEVGRSAFQFRLFAEALREGSYL
                     EATIDHAGETALGPQPEIRRMLVPIGPVAVFGSSNFPFAFSVAGGDTASALAAGNPVV
                     IKAHGSHPLTSRASFDVLSAAAAAQGAPEGTISIVYGTQSGRDLVADPRIRAVGFTGS
                     LSAAQSLLAIIDERDTPIPFYGELSSLNPLVITQAAAEERADELAAGLFGSFTLGAGQ
                     FCTKPGIAFVPTGAAGDAVLDGLVTRSRDAQAQVLLNNRIASSFDEIRARLVTEGRAT
                     VAVEGVAHGFTATPSVLVIEAGDLTASATEEAFGPLVLVVRYSGLDAVYSALDCIPDS
                     LTATILHAPADDITALTRELAPRAGRLVFNGYPTGVRVSWGQQHGGPWPATNSLHTSV
                     GVTAIRRFLRPLAWQNAPEAVLPPELREGAAGIPRRVDGVLTLPA"
BASE COUNT         2020 a         4636 c         4295 g         1988 t
ORIGIN      
        1 gtcgtccgcg ctgagggcgg cgaacgcatc ctcgtcggtc acgtcgtcgc cggcgtagaa
       61 gacggcgtcg gcctgcgtgt actgccgcag gtgctcgacc gcctcaccct tggtggtgga
      121 gcgcgccgag aactcgagca cgtcctttcc ctcgcggatg tagacatcgt tgacctccgc
      181 cttgacctcg ttgcgcgcca cgaggtgggc gatccggctg ttcgcctcgg tcgccagccg
      241 cgtgtgcagc gcgaagccgg cgggcttcgc ctcgagccac acgtcatcga ccgagtcggc
      301 gacctggccc agcaccgtgt tgatcatgtc gacctgcttg agctccgcgg tgtccagaga
      361 gacctgggcc accggcgaat cgagccggat ctcgatcccg tgcgagccga ccagaagcac
      421 cgtgtcgggc aggtcggcga ccgactcaag actggccaac gcccgacccg acaccagagc
      481 gacccgcgtg ttgggcatgg cgagcagccg aagcaccgcg tcgcgcgact cgggcagggc
      541 gcgcgccatc tcgggctcgt cgacctccgg cgcgagggtg ccgtcgaagt cgagagcaac
      601 gagcaaccgc ttggtgcggg cgagctcccg cagagcaccg accagcggtt caggaagccg
      661 cgagtatctc ggcggcgtat cgacgtcaac cacgggtcag tgcgcctttc gactgtcgag
      721 agtccactcg agctcatcga cgagttccgt aggagacatc cgctggaacc gaccgcgctt
      781 ggcctggtcg agagtcgtga ggaagtccgc cgaccaccgg gcgacgtcga actcgtgtac
      841 gcgccggcgc agcgtgcgca tgcgcgaagc ccgctcacgt cggggcatgg cgatggccgc
      901 gaggatcgca tccttgagcc cgtcgatgtc gtgcgggttg atcaacagcg cacgcttgag
      961 ctcgtcggat gcgccggtga actcgctgag gatgagcacc ccgtcttcgt cgaagcggct
     1021 ggcgacgtac tccttggcca cgaggttcat gccgtcgcgc agggccgtca cgagcatgac
     1081 gtcggcggcc aagtagaggg ccaccatctc gtcccgggga tacccgtggt ggtggtagct
     1141 gatggcctgg tggctcatcg tgccgtagtc accgttgatg cggcccacgg tgagctcgat
     1201 ctcgtcgcgc aggttcatgt aggcctcgac gcgctcccga ctggggctgg cgacctgcac
     1261 gagtgtgacg tcctcgacgt tgaggcgacc ttcggcgagc agctcaccga acgccttcat
     1321 gcgatgcgcg atgcccttgg tgtagtcgag tcggtcgaca ccgagcatga cggtgcgcgg
     1381 gttgccgaga ccctcgcgga tctccttggc ccgagcctgg atgcgcggct cgcgcgcgag
     1441 ggcctcgaag ctgcgggcgt cgatcgagat cgggaacgac ttggcgatca cccggcgggt
     1501 gggctcagtg ccgagggaga cctcgatcgc gggacccctc gtcgggttgc cggtgagccg
     1561 gcgcaccgct cgggagaagt tcgaggcatc cgcgacccgc tggaagccga tcacgtcggc
     1621 accgagcagg ccttcgatca cctgggtgcg ccacggcagc tgagagaaga tgccgtacgg
     1681 cgggaacggg atgtggttga agaagccgat ggtgaggtcg ggccgggcgg tcctcagcat
     1741 gcgaggtacc agctgcagct ggtagtcctg cacccagacc gtcgcgccct gcgcgctcac
     1801 ggcggccgtg gcatccgcga accgctggtt gaccgccacg tagctctccc accacacgcg
     1861 gtggtagccg ggcgactcga tgacgtcgtg gtacagcggc cagatggtgt cgttgctgaa
     1921 gccctcgtag tagttctcga tctcgtcggc gctgagggcg atcggcacca gatggatgcc
     1981 gccactctcg aacgggtcga gctcgagatc cggctggccg gcccacccca cccacgcgcc
     2041 gtcggcgtca cgcatgaccg gctcgagtgc tgtcaccagc ccgccgggcg agggcgccca
     2101 ggcgtcaatg ccgtcagcat ccacgaccct gtcgactggc agacgattgg agacgacgac
     2161 gaagtcgtac tccagtgggg cggcgtgcgt cgcggagggc atcgttcctc cacggtaccg
     2221 cagcctcacc ggacggtttg cagagcttca cccagcgttc accgacacac acgccgaaac
     2281 gcgctcgcgt tcactcgcgt ttcctagcgt gggcatcgat gatcagcatc ggaatgagca
     2341 cgtcgtgcgt ctacccgctc ccgaccgagt cggccttccg gctcgcccgt gacgcgggct
     2401 tcgatggcat cgaggtcatg gtcaccaacg accctgtgac gcaggatgcc gccgccctcc
     2461 gctacctctc gaagacctac gggcttccga tcctctccat ccacgccccg gtgctgctgc
     2521 tgactcagct cgtctggggc accaaccccg tgcgcaagct cgaacgctcg gccgaactcg
     2581 ccgcaacgct cggcgcgtcg accgtggtcg tgcacccgcc gttccgctgg caggccggat
     2641 acgcccgccg gttcgaagcg accatccggg agctggccgg cgtgcacagc gtcgagttcg
     2701 cggtggagaa catgttcccg ctgacgctcg gcaaggtcgg cgtgcgcgcg tactcgccga
     2761 gctccgaccc ggtgttcatc gactgcgacg cgatcacgct cgacttctcg cacgcggcgc
     2821 tcggcggccg ggactcactc gagttcgcca tggcgatggg tgaccgcctg cgtcacgtgc
     2881 acctcaccga tggcaccgtt tcgatgcgcg acggccagct cctcgacgag cacctggtac
     2941 ccgggcgggg aacccagccg gtcgccgagg tcgtgcggta cctggcatcc acaggctgga
     3001 gcggctccct ggtcgccgag gtcaacacgg cgggcgcggg gtcggaggac ggccgactcg
     3061 ccatgctgcg cgagacacgg gagttcgcgg catcccagat cgccgcgggc agacgggggc
     3121 ggcgctccgg cgtatcctga ctacgcattc cgcgggttgc gtagagaggc acgatcatcc
     3181 ccatcgtcga caacgccatc tatcgggagg gcgtgcgcca gacgcgtgcc gtgaccctcg
     3241 accagacttt cgaagcgctg cgcgacggag gtgggttcgc gtggatcggc atgtaccgtc
     3301 cgaccgaaga cgagctgcgc gcggtcgcga acgaattcgg ccttcacgag ctcgccgtcg
     3361 aggacgcact cgtcggccac cagcgcccga agctcgagcg ctacggcgag accctgttcc
     3421 tggtactgcg gccggcccgc tacctggatg cacccgaaga ggtcgagttc ggtgaggtgc
     3481 acctcttcat cggtccgaac ttcgccatca cggtgcgcca cgcggagtcg cccgacctcg
     3541 gacgggtgcg caagcgcctg gaacagaacc cggcgctgct cgcgctcggc ccgcaggcga
     3601 tcctctatgc ggtgctcgac caggtcgtcg acgagtacca gccggtgatc gcgggcctgg
     3661 agaacgacat cgacgagatc gaggaccagc tcttccaggg tgaccccgcc gtgtcgcggc
     3721 gcatctacga gctcgccacc gaggcgatgg ggttccagcg cgccacccac ccgctcgtcg
     3781 agatgctcgg gcagctggag agcgggtacg agaagtacca cgtcgacatg gagctgcagc
     3841 gcgacttcag ggacgtgctc gaccacacac ttcagatcgt ggcgcgggcg gactcgttcc
     3901 gcgcgatcct gcagaacgcg ctcaccgttc agtcgacctt ggtgacgcag gcgcagaacg
     3961 acgagatccg caacatgacg caggccagca tcgatcaggg cgaccaggtg aagaagatct
     4021 ccggttgggc ggccatcctg ttcgcgccga ccctcatcgg cgccatctac ggcatgaact
     4081 tccgtttcat gcccgaactg gactggccgc tcggctatcc cttcgctctg ggactcatgg
     4141 ccgtgtcggg cgtggtgctc tacgtggtgt tcaagcgccg gggctggttg tagcgctcgg
     4201 cgtgtcgggg gccgtggcat ccctcagtcc tccacaatga ggacgtggaa cgcttcagcg
     4261 acgatgagaa gcaggcggtg ttcgagctca tgagcgaacg cctcttcgag ttgttcggtc
     4321 ctgggggcag tttccgcatc accttgggtc gtgccagcgc ggatgacgca gtcttcgtct
     4381 ccaccgtcag cgacacgatc gcgtggcagg tcgccgcctc gctcgtgctc gagcctgctg
     4441 ccgcgggaca ccacgccgct gcccagccgg agcacgtcga acgcgacgac ctctggcgcc
     4501 acatcgaagc cgagctgctc gcccagcgcc aggccgccga gtcggtctcg gtcagagttc
     4561 acgcggcctg agcactccgg gcggtctggg caccttcggt cggtctattg acggcacatc
     4621 ggcggccgga ctcctcgatt cggctgttct cctgtgccta cttccgttgt gcaggagagt
     4681 cgagcaaaga tcgtccctcc tgcaaaacgg aagcgacggg aaggaggcgg agcggcggag
     4741 cgctggtgcc ggcgtcccga ctgagccgcc gcgcgctgac tcgctcggcg cggtggcagg
     4801 ctgagcgaga tagacgtatg atgtatgttg tttcatccgc aacgtagggg acgacatgac
     4861 atccaccggg ctcgagcttc gtcccggact gcaccgcgtg caggctccgc tcggcgagcg
     4921 cttcgtcgcg ctgtacctgc tcgtgggacc gaaggcgacc ctcctggtcg acacgggcat
     4981 ccgcgaatcg atcaccgaga accttctgcc ctatctggcg gccacgggcc tcggcccgat
     5041 cacctgggcg atcaacaccc actgcgactt cgaccacacc gggggcaacg gcgcgctcaa
     5101 ggcggcgagc cccgagacgc agctgctcgt gcacgaactc gaccggcgtc tcaccgagga
     5161 cctgcaggca ctcatcgacg tgcgctacgg cgagttccgc gatgccgacg gcttcgacga
     5221 cgcccccgag accaccgagt acctgcgatc cgtctccgac ctcgtggctg tcgaccgcac
     5281 cgtagtcggc ggcgaggtgc tcgacctggg cgaccgggag gtcacgatcc tccacgtgcc
     5341 gggccacagc cccggccaca tcgccgtgca cgacccgctc aaccaggccc tgatcatcgg
     5401 cgacgcgacc ctcggcgcga ccgtgcccac cgccgacaac cggcccgcct tcccgcccac
     5461 ctaccgcgac gtcgacgact acgtcgcctc ggccgagctg ttccgctcgc tcaaccccga
     5521 cctcatcctc acggcgcact acccggtcta cgagggggat gccacgaccg ccttcctcga
     5581 cgagaccgtc gagtacaccc ggctcgtcga ccgcgtgctc gccgacgaac tgcgcgggcg
     5641 caccgccggc gcgacgaccc tcgagctcat caggtcacgg ggcgcggagc tcgggccctg
     5701 gggtgccgcc gccctcgact acgcgatctt cccggtgacg ggcaacctcg aacgcctcga
     5761 ggctcggggc gaggtgtcca gcgaactgat cgacgggcgc cgccactgga tctggacggg
     5821 cacccgatga cggtcaggct gggcgccgtc ggcgctgggt ggtgggcgac gagcaaccac
     5881 ttcccgatct tcgccgcccg cgacgacgtc gaactcgtcg gcgtgtgcgg cctcgggccg
     5941 gaactcgaca ccgtgcgcga ccagttcggg ttcggattct cgaccggaga ctacgagcag
     6001 ctgctcgacg ccgacctcga cgccgtcgtc atcaccacgc cgcacgacct gcactacgag
     6061 caggcggtgc gagcgatcga tcgcgggctg cacgtgctcg tcgagaaacc cgtcaccctg
     6121 aaggccgaca ccgcatggga tcttcagcgc cgcaccgcgg ccgccggcgt gcacttcgtc
     6181 gtgccctacg gctggaacta caaaccgttc accgagcggg cgaaggctct catcgacgcc
     6241 ggtcacctcg gcgagctcca gtacgtgcag tgccacatgg cctcccccac ccgcggcctg
     6301 ttcgcgggtg accccaacga catccgcgcc ctgtgggact cggagaccag cggcccgtcg
     6361 gtgagcacct ggcagtcgcc cgaacacggc ggcggctacg cccacggcca gatcacgcac
     6421 tcgagcgccc tgatgttctg gctgagcggt ctgcgcgccg cgacagtcgc cggccgcacc
     6481 tccctcggcg gtgcgcgggt cgacctgttc gactcggccg tgctgacctt cgacggcggc
     6541 gcgctgggca cgctctcggg ggcggcgacc ctgcccgata tcaaccccta ccaggtcgac
     6601 gtcaagctgt tcggtaccga gggcgtgctg ctgctcgaca ccgagcgcga gcgggtctcg
     6661 ctccaccgct acgacggcca ggcctgggat ctcgacatcg agccgggctc cggtgcctac
     6721 gagtgcatcg aacccccgaa ccgattcatc gatctcatca ccggggcgag caccgtcaac
     6781 aactccgacg cgtccatcgc cgcgcgctcg gtcgagctca tcgacgcact gctgcgatcg
     6841 tctgcggccg gcggggtcga agtggccgtg catccgtgac ccgacagccc accacctcgt
     6901 gcatcaggaa ggcaccatga ccgaaccagc cgccaccccc ggagtgctca gcgggtaccg
     6961 agtgctcgac tgctcgatcg cgatggccgg ccccttcgct gcccagcgct tgggcgacct
     7021 cggggccgac gtgatcaagg tcgagccgac caccggagag tggcagcgcc acaccgcggc
     7081 aggcggggcg acgggcaaca agatcaacgt gtcgttcctg tcgctcaacc gcaacaagcg
     7141 ctccgtcgcg ctcgacctga agagcgccga cggcaaggat gccctgcgcg agctcgtgcg
     7201 caccgccgat gtcttcctgc agaactaccg accgggcgtc gcagcacgcc tgggggtgga
     7261 ctacgagtca ctgcgcgcca tcaacccgcg catcatctac gtgtcgatct ccggctacgg
     7321 cgagtccggt ccctaccgcg atcggccggg ccaggacctg ctggtgcagg cgatgtccgg
     7381 ggcgatgctg tcggcggggc gggcgggtga cgagccgtcg ccggcgggac agtatctcgc
     7441 cgacgcgatc acggcatcca ccgccttcga gggggtgctg gccgcgctgc tgcaccggga
     7501 gcgcaccggg gagggccagc tcgtcaccgt caacatgctc gacgccgtca ccaccctgca
     7561 gatgcaggag ctctcggtgt tcaccgtggg tggcaagcca caggagcgca gcgccgaggc
     7621 gatcgcgcac gtctacatcc gggcgcccta cggggtgttc cgcaccagcg acgggttcgt
     7681 tgctctcgcc ttcgccgacc tgcacctgct gggtgagctc atcgacgagc ccgcgttcga
     7741 gggctggaac agcgaggtcg agggctggac gaaacgcgac gagatccacg ccaagacggc
     7801 cgcgaagctg gtcgccaaca ccagccagca ctggatcgac ctgctcggcg gcgccggtat
     7861 ctggatcggt ccggtctacg gctacgccga tctcgtcgac gacccgcaga tcgcccacaa
     7921 cggaaccttc gtggagtacg accatcccac cgagggccac gtgaagaccc cggggttccc
     7981 ctacacgttc acgaagacac caccgcaggt ctaccgtggt gctccgctgg tcggcgagca
     8041 cacccgcgag atcctggccg aggccggctt cgatcccgag cgcgtggatg ccctgctcga
     8101 gtccggcgct gccgcgacca ccacggcctc cgatccggct cccgtgagct cccgtggctg
     8161 agttcaccgg cctcacctgg gaccacccgc gcgggcgcca agcgctcgaa cgatcggcgg
     8221 cgtccggaac gctgatcgag tggcacgtgc atccgctcga ggggttcgag tcgagtccca
     8281 tcgatgagct tgcggcacgg tacgacgtga tcgtgctcga tcatccgcac ctcggcgacg
     8341 cgctggccac cggcgcgctg cagagtctcg acaccgtctt cgagccggac tggctcgcga
     8401 cgctcacgtg cgtcgggccg agcatccggt cctacgaact cgatggtcac ctgtgggcgc
     8461 tgccgctgga tgccgcaacc caggtgtcgg cgagactccc cgagcgcgtg cccgagaacc
     8521 cggcgacctg gaccgaggtc gtcgaactct cgcaccacca gccggtcgcg ctgtcgctcg
     8581 cgggaccgca cgcctacctc agcttcgcgt cgctcacagt ggccctcggc ggcgacccga
     8641 ccgacccggg cgacaccggt ctcgaggcgc tcgatctgct gcgcgacctg cagagccgag
     8701 cacccgccgg caccgaaagc ctcaacccga tcgggatgct cgagcgcacc cacaccgccc
     8761 gtgacatcgc ctacatcccg ctcgtctacg gctacgtgcc gtacgcccag ggaccggacc
     8821 cgatccactt cggcgacgcc ccgaccgccg tgcgcagcgg ttccaccatc ggcggcacgg
     8881 gactcgccct cagctcgcgc tgcacgccga cccccgccct gctcgagtat ctgcgctggc
     8941 tgctgcaccc ggtgactcag gcaacctaca tccccgacaa cgccggccag ccggcggtgc
     9001 ggtcggcctg gttgtcgccg tcggtgaacg ccgcctccag cgagttctac ctcgccaccc
     9061 tcgcgacgat cgacgccgcc tgggtgcgtc cgcggatcgc gggcttcacc cccttccagt
     9121 ccgaggcgtc gcgcgtgctg cgcgaggcca tcctcggtga gcgctccccc gcgagcgccc
     9181 tcgccgagac cgccgagcgg ttcgccgcgt taggagtgcg cgcatgacca tcaccagcac
     9241 gagcgagcag gtgcgggtcg acaccgacgc acacgtgctc accatcacac tcaaccgacc
     9301 cgagaagctc aactcggtga cccagtcgat gtccgacgcc ctggtcgcga tcatcgagga
     9361 ggcgaacgca gacgacgagg tgcgcgtgat catcctcacc gggtcggggc agcgttcgtt
     9421 ctgcgccggc tcggacatcg cgagcctcga cgcgtatgcg acgccgtggg agttccgcaa
     9481 ccgcaccgac tactgtgacg cgctgcggtc gagccgcaag cccatcatcg cggccatcaa
     9541 cgggtacgcg ttcggcggcg gtctcgagac atcgctgacc tgcgacattc gtctcgcctc
     9601 ggcgacggcg agcttcgcag ctcccgagat caagctcggc tggatcggcg gtggcggcat
     9661 ggccacgttc ctcgcgcact cgatcggggc gagcaacgcg gccgtgatgc tcatgaccgg
     9721 cgaccccgtc gacgcccagc gcgcgctggc ttggggcctc gtgagcgagg tgctcgaacc
     9781 ggatgccctg ctcccacgcg cccgcgaact cgccgcgacg atcgcctccc gaccgcccat
     9841 cgccgccgag accgcgaaat ccaacctgcg cgcggccttc aacatgcctc aggatgaagc
     9901 gatccgatac gagcgcgacc tgcagaccgt caccttcgcc accgaggatg cggccgaggg
     9961 ccgcgccgcg ttcgccgaga agcgccccgg cgtcttccgt cggcgctgag aggaacacca
    10021 tgaccacagc actgctgccg gatgacggca cggcgggcac cctgatcggc cgagtctggg
    10081 acccgcgcgt cagcgggccg agtgtcgtcg ccgtgcgctc cacgggagtc gaggatctga
    10141 gcgggagctt cgccacgatt cgcgatgtgc tggagaacgg cgacccggcg ggggcggtgg
    10201 ctgcggcatc cggcccggtg ctgggaagcg tggctgacct gctcgcgaac accgcgatcg
    10261 ccggccgcga ccggacgttg ccgtggctgc tctcccccat cgacctgcag gtggtgaagg
    10321 cggcgggcgt cacgttcccg gtgtcgatgc tggagcgcat catcgaggag cgcgcccgcg
    10381 gcgagttcga tgcggcggca agcatccggg aggagatcct gaccgcgctc ggcggctcgc
    10441 tgcgcgacct cgtgccggga tcggccgagg cgatgcgggt gaaggagctg ctcatcgagc
    10501 gcggcctgtg gagccagtac ctcgaggtgg gaatcggccc cgacgccgag gtgttcacca
    10561 aggcgcccgt gctttcgacg gtcggcaccg gcgtcgacgt cggcatctac tcggcatcga
    10621 agtggaacaa cccggagccc gaactcgtgc tcgccgtcgc ctcgaccggc cgtatcgtcg
    10681 gagccaccct cggcaacgac gtcaacctca gggacatcga ggggcgcagc gcactgctgc
    10741 tcggcaaggc gaaggacaac accgcgtcgg cggcggtcgg ccccttcatc cgcctgttcg
    10801 acgcgaccta ctcgatggat gacgtgcgca cggccatggt cacgctcgag atcgacggag
    10861 ccgacgatgg cttccggctc gcggcatcca gccccctgag cgagatcagc cgcgacccgg
    10921 tcgacatcgt cgcccaggtg atcggcgacc accaccagta ccccgacggc ttcgtgctgt
    10981 acctcggcac gctgttctcc ccggtcgatg accgcgacgt ggtcggccac ggcttcaccc
    11041 acaagatcgg ggacgtcgtg cgcatcgccg aaccgcgcct cggtaccctg accaatacag
    11101 tgcagttcag cgagaatgcc gagccctgga cgttcggcat cgatgccctg taccgcaacc
    11161 tggccgcccg cggtctgctg aagtaaggac acaccatgct cacgaccacg aaccccgcga
    11221 ccggcgagac caccgactcc ggcattgccg tgaccgcgga tgtcgcggca gtcgtcgacc
    11281 gcgcccagca cgcgttcacg gccctcgccg ccgagccccg cgcctggcgc gccgacctcc
    11341 tggatgcgct cgccgccgcc ctcgaggagc accgcgacga cctggtggcc ctcgccgact
    11401 cggagacggg tctgggcgtg gcgcgcctga ccggggaggt ggggcgcagc gcgttccagt
    11461 tccggctgtt cgccgaggcc ctgcgcgagg gttcctacct cgaggcgaca atcgaccacg
    11521 cgggcgagac ggcgctcggc ccgcagccgg agatccgtcg gatgctcgtg ccgatcgggc
    11581 ccgttgccgt gttcggttcg agcaacttcc cgttcgcctt ctcggtggcg ggaggcgaca
    11641 ccgcctccgc actcgccgcg ggcaaccccg tcgtcatcaa ggcgcacggc tcgcacccgc
    11701 tcacctcgcg ggcgtcgttc gacgtgctgt ccgctgccgc ggctgcgcag ggtgctccgg
    11761 agggcaccat ctcgatcgtc tacggcacgc agtccggccg cgacctcgtg gccgatccgc
    11821 gcatcagggc cgtggggttc accggatcgc tgtcggcggc gcagtccctc ctggcgatca
    11881 tcgacgagcg ggacacgccc atcccgttct acggggagct gtcgagcctc aaccctctcg
    11941 tgatcacgca ggccgctgcc gaggagcgcg ccgacgaact ggccgccggt ctgttcggat
    12001 cgttcacact gggtgcgggc cagttctgca ccaagcccgg catcgcgttc gtgccgaccg
    12061 gggcggccgg tgacgccgtg ctcgacggtc tcgtcacccg ctcgcgcgat gcgcaggcgc
    12121 aggtactgct caacaaccgc atcgccagct cgttcgatga gatccgcgca cggctcgtca
    12181 ccgaggggcg cgcgacggtc gccgtcgagg gcgtcgcgca cgggttcacc gcgactccgt
    12241 cggtgctcgt catcgaggcc ggggacctga ccgcctcggc caccgaggag gctttcggcc
    12301 cgctggtgct cgtggtgcgc tactcgggcc tcgatgcggt ctactcggcg ctcgactgca
    12361 ttcccgactc actcacggcg acgatcctgc atgcccccgc cgacgacatc acagccctga
    12421 cccgcgagct cgcgccgaga gcgggacgcc tggtcttcaa cggctacccc acgggcgtgc
    12481 gggtgtcgtg gggccagcag cacggcgggc cgtggccggc gaccaactcc ctgcacacct
    12541 cggtgggggt caccgcgata cggcggttcc tgcggccgtt ggcctggcag aacgcgcccg
    12601 aggcggtgct gccgcccgag ctccgcgagg gtgccgcggg catcccgcgt cgcgtcgacg
    12661 gcgtgctgac cctgcccgcc tgaaccgcgg cggggaacag cgctgcacct ttcacgctcg
    12721 acggcatact ctgactgact cctgttgtgc aggagcgccg agatcttctc gcatcaagag
    12781 gcagacggtc ccgttatgca ggatccgcga gtcttgctcg gtgatcctgc ataacgggag
    12841 ggtgctgaag atatctgatt cgtcgcaacc tcaggcgcgc cgagctgccc gcgtgacctg
    12901 aggcccgacc ccgcgaaggc gcgcgtcccc gggagctat
//
LOCUS       DAOSOU010000034        12928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4839_length_12928_cov_6.227841, whole genome shotgun sequence.
ACCESSION   DAOSOU010000034 DAOSOU010000000
VERSION     DAOSOU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12928
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4839_length_12928_cov_6.227841"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3172
                     /locus_tag="PLW15_03215"
     CDS             <1..3172
                     /locus_tag="PLW15_03215"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOT33442.1"
                     /translation="DGDSVQLLGQASNPEKGAVIDTQADTISYQAGDYSAGTDTFTYT
                     VIDSLGARSTGTIRVGISPRLEGARNPVAIVDEVTVRPGVTVSVQVLANDSDPDGNPL
                     TIVSAEPNDTVTTAEIVGDLVQVTPPRAPGTYGVIYSIANASGGSSQNFIRITVKADA
                     ALAYPLVSDTVLTLSDVLDRDQVTVDVLANVFFADGDPRTLGLSIYPGYGANATVTSG
                     KRVIVTVGKKSQIIPFKVTHPDDPSVVSYGFIRVPGSDDALPQIDRRAPALRVNSEDT
                     LTIDLNDYVIAVGGKQVRVTDANSVRATHSDGSNLVVDDDTLAFTSADKYFGPASISF
                     EVTDGTSATDPNGRTAILVLPITVLPRDNQPPVFRGAVISFEPGQEKVIDLLKLTNYP
                     YPDDLAELAYSVIDPLPVGFSYALNGSQLTLRADDDAVKGSATAMTLGVKDDLSVGQS
                     GRIELSVVQSTRPLVRPGADQAIVKRSSTTTVDVLANDEATNPFAGKPLRVVAIRGLD
                     GDALPAGVEIVPSANKSSLSVTVSSTAAPGDVTLQYQVADATNDPDRFVWGLVTISVQ
                     DRPDPVTNLTATGFGDREISLRWNAGAFNNSPITGYRVVVSTTSGSTFTTVTCAGTTC
                     TVPTPGNGSANAVRFSVVSTNAIGDSDAVSTGSGVWSDIVPAAPSGVSAEPLDHGLRV
                     TWNAVPTPSGGTPVSTYVVDVGGVSTSVGCSTATCTTSVVDSSLANGTLVTYSVSARN
                     DAYGPLASWNSTSQQGTPAGAPLAVTPPIATSTSDTSIALDWSGTFSANGRAVSDYTA
                     AIYTGAGPSCAADGTVNDNGATLFAAGTGTSTIFSGLSPEVEYTLVVFAYNGQGCTQS
                     QTVIARTPPSVVVDLSVSGPSANGSVFDFVLQGIDLGGGMVSSGYSVYYRLNGGTEYG
                     PVSIPGFLAAEPLQFGQSVSVELRACRDYGGQLVCQPTMSSAFPLGVPVDPQVIGPLT
                     FTQDDSGDPQNDGTFAWLGFPSGSYELVEYACGTAPGDGTFTVAGSDPTCHVSANPAQ
                     TPWLTIRVTANGGQTYTISYNGFDYD"
     gene            3198..4169
                     /locus_tag="PLW15_03220"
     CDS             3198..4169
                     /locus_tag="PLW15_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT33443.1"
                     /translation="MTMTPEQATWFSDIFNRLVANVDQVLLGKTFVIRLGFTALLSEG
                     HLLLEDFPGTGKTSFARAMAQSVAGTTSRIQFTPDLLPGDITGVSIYDQSKSAFEFHA
                     GPIFANIVLADEINRASPKTQSALLEVMEEAHVTVDGVTHPVGAPFMVIATQNPIEQA
                     GTYRLPEAQLDRFIMKASIGYPDHSSTLRILEGAGNKAHEIVIPPIVTADVVVEMGQL
                     ARTVHVDPTINDYVSRLVEATRTASEVRLGASVRGALALIRTAKTLAAASGRHYVIPD
                     DVKALAEPVLAHRLIIDPEAEFDGVTAPSIMGQILIETPPPSERQAV"
     gene            4403..5605
                     /locus_tag="PLW15_03225"
     CDS             4403..5605
                     /locus_tag="PLW15_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT33444.1"
                     /translation="MFGRVAAVVTPLGWTVVLLVPLTLGFGYGLGWIELIVVGFAGLA
                     MLVIATLYLIGRSPVSIALTVEHSRVVVGEPAAGQLVATNPGRRRALGVTVEVPVGLG
                     LAELVLPGLARGASVQRQFPIPTARRGVVPVGPVRTVRADPVGLVRRELIWADVAELF
                     VHPRTIGIPSLSTGLIRDLEGKPTRDLSNNDVSFHALREYLPGDERRYIHWKSTARTG
                     KYMVRQFEETRRSHLVVALSLASSDYGTDAEFEMAVSVAGSLGARAIRDARTVSVVAS
                     EYTPQFAKRKVFAVRLLSTLTRSRLLDDLAVVEKSETALSITDVAKVAGDQATGVSVA
                     FLVCGSTVNPQELRAASVAFPVGVEVIAIVCDPDRTPGLRRVSGLSVLTIGYLEDLQK
                     SLARSVAA"
     gene            5605..7890
                     /locus_tag="PLW15_03230"
     CDS             5605..7890
                     /locus_tag="PLW15_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HOT33445.1"
                     /translation="MSPRPSAIVVAVNTVMLWLATVVAASALWPIYASSAFLVMVAVA
                     LAVGTLIAILGAIFRWPAFVVMLATVVGFFAVGVPVAVPSKTQFGVLPTFEGLIDLVA
                     GVALGWKQLLTITLPVGDYEALLVPAFVLVLLSTVVGLTVALRAQWGELGVLAPIVVF
                     IVATAFGPEFPDRPLIAPIALMVIVLFWLVWFRWYRRRTAIRMLVSRTGDVAAGGAET
                     PIAGLRTVVAAGVIMAVASVGAVAGAAALPPSADRTVLRTTIVQPFDPRDYVSPLAGF
                     RSFWRPNQVDASQFTVDGLADGERIRLATMDTYDGIVFSVGSGDVSTESGSFTRVPYR
                     FDQSGVSGDRVSVAVTIDAYSGVWLPTIGQFESIEFAGDDAADLRNAFYYNDVTGTAA
                     VIGGLSQGDSYTLTAVVPRQPTDDQWDTLTPGSATVPTPSSVPAEVATKLDEYTADVD
                     GAGPKLVAMIAGLTAEGYISHGVGADEPASRSGHAADRIAELLSAPRMIGDAEQYAVA
                     AALMANQLGFAARVVLGFQPQSGVVRGSDLSAWIEVSTAQFGWVALDPTPPLREIPDE
                     QPPDNAQVSRPPTIVPPPVVDSERFDRQTTPDSEQELPPDLDPVLQVLLVLLRVIGWV
                     LLAAAIVVAPFLLIIAAKLRRRRLRRRAPSTIEQISGGWQEFEDAVIDHGLSPAASAT
                     RSEVAAIAGGVQSQVLAAVADRAIFSPDEPAASDVDSVWRAVDELHASLDEGLTRWQR
                     LKARISLRSLGGYSVRDLFKR"
     gene            8614..8895
                     /locus_tag="PLW15_03235"
     CDS             8614..8895
                     /locus_tag="PLW15_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT33446.1"
                     /translation="MTSAGLLGRNPMPEPGEYFEALVAISDPGKSVSKTHLEFGQDGG
                     AFWISDRYSGNGTVVREPDAQPRRCEPGKRYRIVRGTRVDIGEQFFVVS"
     gene            8986..11772
                     /locus_tag="PLW15_03240"
     CDS             8986..11772
                     /locus_tag="PLW15_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HOT33447.1"
                     /translation="MTPVPLELPLPPERPAPFRFPIIAASAPVILAVGIFVVTGSAFT
                     LAFAALGPATAIAGYFDSRWSARKLRRRETTRFAIDRDRVGDEIERRHADERTRLFDA
                     SPDPARLVTHEGPDPHRWRGDGRSPILVVVGTASIPSMLPVDAPAARISGPPSDADRA
                     IADLVDRARVLEGAPMTVDARLGIGVTGPPAFARAIARGIAVQLARRLSPSHCWRSAS
                     SAPYDLWLDDLPHEAAAASLPGSVVEFGQKGEAVPLAIVAVAETPAHLPGACSIVLGV
                     VDGQLRILSHPDADHRRPLHPLPVTEIEAAAWALRLSRDAAREGMTSPHALLPDAVEL
                     TPLLRSAEAGDVASLACEPGVDAGGSVTLDLVAHGPHAVIGGTTGSGKSELLIAWILA
                     MAASRSPQHVSFLLIDYKGGATFAALARLPHTVGIITDLDAMMAARALDSLRAEVRHR
                     ERLLVTSGARSIEQVDSLPRLVIVVDEFAAMLAEHPDLHGLFADLAARGRSLGMHLVL
                     CTQRPAGAVRDGVLANADLRVSLRVNNRADSSAVVGCDDAAGIPLTARGRGVLRLAGE
                     APRSVQFALASGSDVALVTQRWSQSPSPRRPWCEPLPAVLKAAALPRDGIGCFGLVDL
                     PSEQRQEPAIHSPEAEGALLVLGSPASGKSTALRALAAGHPGIRVVPAEPDGAWDVIA
                     DLVAALDSPASTATCVVLDDLDALVPRFTGEYRAAFVDLLARVLREGPGRGITALLSA
                     QRITGESQGLATLVPGRLLLRHPSRQDFVIAGGEGGQFVAALPAGRGLWRGQWMQVVA
                     DPPPLPATGPTVAPLLDPRRARAIVTSRVAPLLARWPSAIALSDAGPELRSLALPGVT
                     IVGDLDEWQSRWGAVAALRTQADIILDGCTPADFRAITRSRQLPPPLAPGQCWQLNED
                     GSARRVRLDPPTRD"
     gene            complement(11782..12900)
                     /gene="ald"
                     /locus_tag="PLW15_03245"
     CDS             complement(11782..12900)
                     /gene="ald"
                     /locus_tag="PLW15_03245"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOT33448.1"
                     /translation="MKVAVPREIKNNEFRVAITPAGVHDLVGHGHEVLIETGAGLGSS
                     IPDDAYVAAGASIAPDAATVWAAAELLLKVKEPIESEYGYFRSDLLLFTYLHLAAEPE
                     LTTALTTSGVTAIAYETVQLPNRALPLLAPMSEVAGRLAPIVGANAMLKPNGGPGLLV
                     PGVPGTHNARVVVLGGGVAGTNAVSVAVGLGAEVTVLDTNIQRLRELDALYAGRVHTV
                     ASNGFEIERALLTADLVIGSVLVPGAKAPKLVSNDLVSRMKPGSVLVDIAVDQGGCFA
                     DTRPTTHADPTFAVHGSLFYCVANMPGAVPNTSTYALTNATMPYVRAIANHGWLDALR
                     ADPALALGLNSHAGSIVNAPVAAAHGLKHTELAAILAA"
BASE COUNT         2004 a         4436 c         4249 g         2239 t
ORIGIN      
        1 ggatggcgac tcggtgcaat tgctcggtca ggccagcaac ccggagaagg gtgcggtcat
       61 cgacacccag gctgacacga tctcctatca ggccggcgac tactcggcgg gaaccgacac
      121 cttcacctac accgtcatcg actccctcgg tgcacgctcc accggcacca tccgcgtggg
      181 catcagccca cggctcgagg gcgctcgtaa cccggtcgcc atcgtcgacg aggtcaccgt
      241 gcggcccggc gtgaccgtgt cggtgcaggt gctcgccaac gactcggacc cggacggaaa
      301 cccgctcacg atcgtctccg ccgagcccaa cgacacggtg acgactgcgg agatcgtcgg
      361 ggatctcgtg caggtcaccc cgccccgcgc ccccggcacc tatggcgtca tctactcgat
      421 cgccaacgcc tccggcggca gcagccagaa cttcatccgc atcaccgtga aggccgacgc
      481 ggcgctggcc tatccgctcg tatccgacac tgtgctcacc ctgtccgacg tgctcgatcg
      541 tgaccaggtc acggtggatg tcctggcgaa cgtcttcttc gccgacggcg atccgcgcac
      601 gctcggcctg tcgatctacc ccggctacgg cgcgaacgcg acggtgacca gcggcaagcg
      661 cgtcatcgtc accgtcggca agaagagcca gatcatcccg ttcaaggtca cccacccgga
      721 cgacccgtca gtggtcagct acggcttcat acgggttccg ggatccgatg acgccctgcc
      781 ccagatcgat cgccgcgctc ccgctcttcg cgtcaatagc gaggacacac tgaccatcga
      841 tctcaatgac tacgtgatcg cagtcggcgg caagcaggtg agggtcaccg acgcgaacag
      901 tgtgcgggcg acccactccg acggcagcaa cctcgtggtg gatgacgaca ctctcgcctt
      961 tacctcggcc gacaagtact tcgggcctgc atcgatttcc ttcgaggtga ccgacggcac
     1021 ctcggccacg gatccgaacg gccgcaccgc gatcctcgtg ttgccgatca cggtcctgcc
     1081 ccgagacaac cagcctccgg tcttccgcgg cgccgtcatc agcttcgagc ccggtcagga
     1141 gaaggtgatc gacctgctca agctgaccaa ctacccctat cctgacgacc tggctgagct
     1201 cgcctactcg gtcatcgatc cgctcccggt cggcttcagc tacgccctca atggctcgca
     1261 gctcaccctg cgcgccgacg atgacgcggt caagggctcg gcgacggcga tgacccttgg
     1321 agtgaaggac gacctgtcgg tgggtcagtc cggtcgaatc gagttgagcg tcgtccagtc
     1381 cacgcggccg ctggtgcggc cgggcgccga ccaggccatt gtgaagcgca gctcgacgac
     1441 gaccgtcgac gtgctcgcga acgacgaggc gaccaacccg ttcgcaggca agcccctgcg
     1501 ggtcgtcgcc attcggggcc tcgacggcga cgcccttccc gcgggtgtgg agatcgtccc
     1561 gagcgccaac aagagttcct tgtcggtcac ggtctcctcg actgccgcgc cgggcgacgt
     1621 cacccttcag taccaggtgg ctgacgcgac caacgatccg gaccggttcg tgtggggcct
     1681 cgtcacgatt tccgtgcagg atcgcccgga tccggttacg aacctgacgg ccacgggctt
     1741 cggtgatcgc gagatctcgt tgcgctggaa tgccggcgca ttcaacaact cgccgatcac
     1801 cggctatcga gtcgtcgtga gtaccacctc ggggtcaacc ttcaccaccg tcacctgtgc
     1861 aggaaccacc tgcacggtgc ccacccccgg aaacggatcc gccaacgcgg tgagattctc
     1921 cgtggtgtcg accaacgcca tcggcgactc cgacgccgta tccaccggtt caggtgtctg
     1981 gtccgacatc gtgccagcag cgccaagcgg cgtctcagcg gagcccctcg accacggact
     2041 gcgcgtgacc tggaacgcgg tcccgacacc ctccggcggc accccagtat cgacctacgt
     2101 cgtcgacgtg ggcggggtgt cgacgtctgt cggatgctcc accgcaacct gcacgacatc
     2161 cgtcgtcgac tcttccctcg ccaacggcac cctcgtcacg tactcggtca gcgctcggaa
     2221 cgacgcgtac ggaccgctgg cgtcgtggaa ctccacgtcc cagcagggca cgccggcggg
     2281 cgccccactg gcggtcaccc caccgatcgc gacgtcgacg agtgacacga gcatcgccct
     2341 ggattggagc gggacgttct ccgccaacgg gcgtgcagtg tccgactaca cggcagcgat
     2401 ctacacgggc gccggcccga gttgtgctgc cgacggcacc gtcaacgaca acggagcaac
     2461 cctcttcgcc gcgggaaccg gcacgtcgac gatcttcagc ggcctgtcac ctgaagtcga
     2521 gtacacgctg gttgtcttcg cctacaacgg acagggctgc acccagagtc agaccgtgat
     2581 cgcccgcacg cctccgtcag tcgtggtcga cctctcggtc tccgggccgt cagccaacgg
     2641 gtcggtgttc gacttcgtcc ttcagggaat cgacctcggc ggcggcatgg tctccagcgg
     2701 ctactccgtg tactaccggc tcaatggcgg cacggagtac ggccccgtta gcattcccgg
     2761 attcctggcg gccgagcctc tccagttcgg ccagtccgtg tcggtggaac tcagggcgtg
     2821 ccgcgattac ggcggacagc tcgtgtgcca gccgacgatg tcgtcggcct tcccgttggg
     2881 tgttcccgtc gacccccagg tcattggccc gttgaccttc actcaggatg actcgggcga
     2941 tccgcagaac gatggaacgt tcgcgtggct aggctttccc tccggaagct acgagctcgt
     3001 cgagtacgcc tgcggcacgg caccggggga tggcacgttc accgtcgcgg ggagcgatcc
     3061 gacctgccac gtctcggcga accccgcaca gacaccgtgg ctcaccatcc gggtgacagc
     3121 caacggcggg cagacctaca cgatttccta caacggcttc gactatgact gacagcatca
     3181 cgaatgaagg gcatcccatg accatgacac cggagcaggc gacgtggttc tccgacatct
     3241 tcaaccgcct ggtggccaat gtcgaccagg tactgctggg caagacgttc gtcatccgac
     3301 tcggattcac cgcgctgctc agcgagggac acctgctgct cgaggacttc ccgggaaccg
     3361 gcaagacgtc cttcgcgagg gcgatggcgc agagcgtcgc gggcacgacc agccgcatcc
     3421 agttcacccc cgacctgctc cccggcgaca tcaccggggt gagcatctac gaccagtcga
     3481 agtcggcatt cgaattccac gcggggccga tcttcgcgaa catcgtgctt gccgacgaga
     3541 tcaaccgggc gagcccgaag acccagtcgg cgcttctgga ggtgatggag gaggcacatg
     3601 tcaccgtcga tggcgtcacc caccccgtag gggcgccctt catggtgatc gccacgcaga
     3661 atccgatcga gcaggccggc acctatcgcc tgcccgaagc ccaactcgac cgcttcatca
     3721 tgaaggcatc gatcgggtat cccgatcatt cgtctaccct gcgaatcctc gagggcgccg
     3781 gcaacaaggc tcacgagatt gtgattcccc cgatagtgac ggcggatgtg gtggtcgaga
     3841 tggggcaact ggctcgcacg gtccacgtgg atccgaccat caacgactat gtgagtcggc
     3901 tggtcgaggc cacccgcacg gcgtccgagg ttcggctggg cgccagcgtg cgcggagcgc
     3961 tcgccctcat tcgcacggcg aagacgctgg cggcggcgag cgggcgccac tacgtcattc
     4021 cggacgacgt caaggcgctc gccgagccgg tgctcgccca ccgactgatc atcgacccgg
     4081 aggcggagtt cgacggtgtc accgcgccca gcatcatggg acagatcctg attgagacgc
     4141 ccccgccgag cgagcggcaa gccgtgtaat ggctcgggcg aaggtgcccg gcggcgtgtc
     4201 atccgttcgc ggatcgacac gctcttcgtc gcgccccgct ggcagcacga ccgggtcgcg
     4261 cacagacctt cactcgacga cgtcaaccaa ggggctcacc aatgcgcgaa cgcggatcgt
     4321 cggtgaccgc acggggttcc tcgctgacgc gatagtcacc gttgtccggc tcgcaacggc
     4381 attcggtgag gctctcagcc gcgtgttcgg gcgagtcgct gcggtggtga ctccactcgg
     4441 gtggaccgtc gtgctgcttg tgccgctgac gctggggttc ggctacgggc tgggctggat
     4501 cgagctcatc gtcgtcgggt tcgcgggact ggcgatgctc gtcattgcga ccctctatct
     4561 catcgggcgg agcccggtga gcatcgcact gaccgtcgaa cacagccgcg tcgtcgtcgg
     4621 agagccagcg gcaggccagc ttgtggccac caaccccggc cggcgccgcg ccctcggcgt
     4681 gacggtggag gtaccggttg gcctcggatt ggccgaactg gtgcttcccg ggctcgcgcg
     4741 cggcgccagc gtgcaacgcc agttccccat ccccaccgcg cgtcgaggtg tcgtgccggt
     4801 gggacccgtg cgaactgtgc gcgccgatcc cgtggggctc gttcgccgcg aactgatctg
     4861 ggcggatgtc gccgaactgt tcgtgcatcc gaggaccatc ggcatcccga gcctcagcac
     4921 cgggctgatc agggacctcg aaggcaagcc cacgagggat ctgtccaaca acgacgtgtc
     4981 gttccatgcc ctccgtgagt accttcccgg ggacgagcgg cgctacatcc actggaagtc
     5041 cacggcccgc acgggcaagt acatggtgcg gcaattcgag gagacccgac gcagccatct
     5101 cgtggttgcg ctgagtctgg cgagttccga ctatggaacc gacgccgagt tcgagatggc
     5161 ggtgagtgtt gcgggttcgc tgggtgctcg cgccattcgg gatgcccgta cggtctcggt
     5221 tgtggcgagc gagtacacgc ctcaattcgc caaacgcaag gtgttcgcgg tgcgattgct
     5281 gtcgactctg accaggtcca ggcttctcga cgatctcgcc gtggtcgaga agtcggagac
     5341 ggccctgagc atcaccgatg ttgccaaagt cgcgggggat caggcaaccg gtgtctcggt
     5401 cgccttcctc gtctgcgggt ccacggtcaa cccgcaggaa ctgcgcgcgg cttcggtcgc
     5461 gttcccggtc ggcgttgagg tgatcgccat cgtctgcgat cccgaccgaa ctccgggcct
     5521 gcgccgggtc tcgggcctca gcgttctcac catcgggtat ctcgaggacc ttcagaagtc
     5581 tctcgctcga tcggtggccg cctgatgtcc ccccggccga gcgcgattgt ggtggcggtc
     5641 aacaccgtca tgctgtggtt ggcgacagtg gtggcggcca gcgcgctctg gcccatctac
     5701 gcgagttcgg cattcctcgt gatggtggcg gtcgccctcg cggtgggaac cctgatcgcc
     5761 atcctcggag cgatcttccg gtggccggcc ttcgtcgtga tgctcgcgac cgttgtggga
     5821 ttctttgctg tcggagtgcc ggttgccgtg ccgtccaaga cccagttcgg cgttctgccg
     5881 accttcgagg ggcttatcga tcttgtcgcc ggtgttgccc tcgggtggaa gcaacttctc
     5941 acgatcaccc ttcccgtcgg tgactatgag gcgttgctgg ttcccgcgtt cgtactggtg
     6001 ctcctgagca ccgtcgtggg cctgacggtg gcgctgcgcg cgcagtgggg cgaactcggt
     6061 gtgctcgccc cgatcgtcgt gttcatcgtt gccaccgcgt tcggtcccga gttccccgac
     6121 cggcctctga tcgccccgat cgccctcatg gtcatcgtgc tcttctggct cgtgtggttt
     6181 cggtggtatc gacgccgtac ggcgatcagg atgctcgtca gccgaaccgg ggatgtggcc
     6241 gcgggcggcg cagagactcc gatcgccggc ctgcgcacag tggtcgctgc tggtgtcatc
     6301 atggcggtgg cgtccgtggg ggctgtcgcg ggtgccgctg cgcttcctcc gtccgccgat
     6361 cgcactgttc ttcgcacgac catcgttcag cccttcgatc cgcgggacta cgtcagtccg
     6421 ctcgccggat tccgcagctt ctggcgcccg aaccaggtgg acgcgtcgca gttcacggtg
     6481 gacggcctgg cggacgggga gcgcatccgc cttgcgacca tggacaccta cgacggcatc
     6541 gtcttctccg tcgggagcgg cgacgtcagc acggagtcgg gctcattcac gcgggttccc
     6601 taccgattcg accagtcggg cgtgagcggc gatcgggtga gcgtcgccgt caccatcgac
     6661 gcctactccg gagtctggct acccacgatc ggtcagttcg agtcgatcga gttcgccggt
     6721 gacgacgccg ccgatctgcg caacgcgttc tactacaacg acgtgacagg aaccgcggca
     6781 gtcatcggag gcctgagcca gggcgattcg tacaccctta ccgctgtggt tccgcgccag
     6841 ccgacggacg accagtggga cactctgacg cccggctcgg ccactgtgcc caccccgagt
     6901 tcggtccccg cggaggtagc gaccaagctc gacgagtaca ccgcggatgt cgatggtgcc
     6961 ggtccgaagc tcgtcgcgat gatcgccggc ctcaccgccg aggggtacat cagtcacgga
     7021 gtcggcgcag acgagcccgc cagccgctcc ggccacgctg cggaccgcat cgccgagctg
     7081 ctcagtgcgc cgcgcatgat tggcgacgcc gagcagtatg cggtcgccgc agcgctcatg
     7141 gccaatcagc tcggctttgc ggcccgcgtc gtcctggggt tccagcccca gtccggggtg
     7201 gtgcgggggt cagatctctc ggcatggatc gaggtcagca cggcccagtt cgggtgggtg
     7261 gctctcgacc ccactccgcc gttgcgggag atcccggatg agcagcctcc cgacaatgcg
     7321 caggtgtcgc gtccgcccac gatcgttcct ccgcccgtcg tggactccga acggttcgac
     7381 cgacagacca ctcccgacag cgagcaggag cttccgcccg atctcgaccc ggtgctccag
     7441 gtcctcctgg tgctgctccg cgtcatcggc tgggtgctgc tggccgctgc aatcgtcgtg
     7501 gcgccgtttc tgttgatcat cgccgccaag ctccggcggc ggcgtctgcg caggcgcgct
     7561 cccagcacca tcgaacagat cagcgggggt tggcaggagt tcgaggacgc cgtcatcgat
     7621 cacggactca gccccgctgc atccgccacg cgcagtgaag tggctgctat cgcgggcggt
     7681 gtccagtccc aggttctggc ggccgttgcc gatcgggcga tcttctcgcc ggatgagccg
     7741 gcggcatccg atgtcgacag cgtgtggcgg gcagtggatg agctgcacgc ctcgctcgac
     7801 gaggggctca cccgctggca gaggctgaag gcacgaatct cgcttcgctc actcggtggt
     7861 tatagtgtca gagacctgtt caagcgatag gactgcactg tgaactgctc ctactgcgcg
     7921 acagagcttc cgttgggggc gatgttctgc ggcgaatgcg gccgatccgt cgcgagcgcg
     7981 gcgacgccga gagccgcgcc cgcgccgacg gccacgccgg tgcacgaggg cgccgagctg
     8041 gtcgagccga ggtcgtcggt tcgtgatgaa gcgcgaattc tgccggagcc ggagccggag
     8101 cctgagcctg agctcgcgcc tgagcccgtg cctgagctcg cgccggaaac ggcgcctgag
     8161 ccggaacccg agcctgaggt cgagccggag cccgagcccg aggtcgccct tcaacccgag
     8221 cctgaggtcg cgcgcgagcc ggagcccgaa cccgcgaatg acgtcgcgcc cgaactgcag
     8281 cccgagccgg atgacacgcc gtcccagagc gccccgccgg agtcgtgtgc acagtgcggc
     8341 actcggcttg ataccgccga catcttctgt cccgagtgtg gcttcgttcg acagtccgcc
     8401 gccaatcggc cgcgcgacac cgtcgccctg gatccatttc cgtggggtac tccggctggc
     8461 gcgccactgc cagcccccgc agtcgagatc atcgagaact tgggggattc tgcacgcgtt
     8521 cagtatgacg acatcgatga cacgcgcatc gtggatcgaa gtcggagtgg ggagcgtttc
     8581 gtccttcagt tcagcacggg cgagagtgtg agtgtgacca gcgccggtct gctcgggcgc
     8641 aatccgatgc ccgagccggg cgaatacttc gaggcgctcg tcgccatctc cgatcccggc
     8701 aagtcggtat ccaagacaca cctcgagttc ggccaggacg gtggtgcgtt ctggatcagc
     8761 gaccggtact ccggcaacgg aaccgtggtg cgcgaacccg atgcccagcc ccggcgctgc
     8821 gagccgggca agcgctaccg catcgttcgt ggcacgcggg tggacatcgg cgagcagttc
     8881 ttcgtggtca gctgacacct ccgcaccatc cggtgctgca ccgatttctg cacgaagcag
     8941 ggcgatgccg ttcggacgag aacgcccgca tggtgtcgtg ggaaggtgac tccggtaccg
     9001 ctcgagctgc ccctcccgcc cgaacgtccg gcgcccttcc gatttccgat cattgccgcc
     9061 tccgcgccgg tgattctcgc cgtcggaatc ttcgtcgtca ccggatcggc cttcactctc
     9121 gcattcgcag cgctcgggcc tgccactgcg atcgccggat acttcgattc acggtggagt
     9181 gcccgcaagc tgcgtcgtcg cgagaccacg cgcttcgcga tcgatcggga tcgcgtcggg
     9241 gacgagatcg agcggcgcca cgccgacgag cgcaccaggt tgttcgacgc gagcccggat
     9301 cccgcacggc tcgtcacaca cgaggggcca gaccctcatc gctggcgcgg cgatgggcgc
     9361 tctccgattc tggttgtcgt cggaaccgca tcgattccga gcatgctgcc ggtggatgcg
     9421 cctgccgccc gcatttccgg tccgccgtca gatgccgatc gcgcgatcgc cgacctcgtc
     9481 gatcgcgccc gggtgctcga gggagcaccg atgacagtcg acgcgcgtct cggcatcggc
     9541 gtgaccggcc cgcccgcgtt tgcccgggcg atcgcccgcg gaatcgccgt ccagctcgca
     9601 cggaggctct ccccgtcaca ctgctggcgg tcggcgagct ctgcacccta cgacctgtgg
     9661 ctcgatgacc tgccccacga agcagcggcg gcgagtctgc ccggctcggt cgtcgagttc
     9721 ggtcagaagg gtgaagcggt gccgctggcc atcgttgctg ttgccgagac accagctcat
     9781 ctccccggcg cctgctccat cgtgctcggt gtcgtggacg ggcaactgcg tatcctgagt
     9841 catcccgacg ccgaccatcg ccggccgctc cacccactgc cggtaaccga gatcgaggcg
     9901 gcggcgtggg cccttcgcct gtctcgggat gccgcccgcg agggcatgac gtcgcctcat
     9961 gccctgctgc cggatgccgt cgagctcacg ccgctcctgc ggtcggctga ggctggagat
    10021 gtcgcctccc tcgcgtgcga gcccggtgtg gacgctggcg ggtcggtcac gctggatctg
    10081 gtcgcccacg gtccgcacgc ggtgatcggg ggcaccaccg gaagcggcaa gagcgagctg
    10141 ctgatcgcgt ggatactggc gatggcggcg tcccgatcac cgcagcacgt cagcttcctg
    10201 ctcatcgact acaagggcgg ggcaacgttc gcagctcttg ctcggttgcc gcacaccgtc
    10261 ggtatcatca ccgatctgga cgcgatgatg gcggcgcgcg cgctcgacag cctgcgggcc
    10321 gaggtgcgtc accgggagcg tctgctggtg accagtgggg ctcgttccat cgaacaggtc
    10381 gactcgcttc cccggctggt catcgtggtc gatgagttcg cggcgatgct cgccgagcat
    10441 cccgatctgc acgggctgtt cgccgatctc gctgcgcgag ggcgctcgct gggcatgcac
    10501 ctcgtgctgt gcacgcaacg cccggcgggc gccgttcgcg acggggtact ggcgaatgcc
    10561 gacctgaggg tgtcccttcg ggtgaacaac cgagccgaca gctcggccgt tgtggggtgc
    10621 gacgacgccg cggggatccc actcacggcg cgcggacggg gcgtcctcag actcgccggc
    10681 gaagcaccac gctcggttca gttcgccctc gcctcagggt ctgacgtcgc gctcgtgacg
    10741 caacgatgga gtcagtctcc gtcgccgcgt cggccgtggt gcgagcctct tcctgccgtg
    10801 ctgaaggctg cagcccttcc gcgcgacggc atcggatgtt tcggcctcgt cgacctgcca
    10861 tccgagcagc gtcaggagcc ggccatccac agccccgagg cagagggtgc tctgctcgtg
    10921 ctcggttcgc ccgccagcgg caagtcgacg gcactgcgcg ctctggccgc cggtcaccct
    10981 ggaattcggg tcgttcccgc tgagcccgac ggagcatggg atgtgatcgc cgatctcgtc
    11041 gccgccctgg actccccagc gtccacggcg acgtgcgtcg ttctggacga cctcgacgct
    11101 ttggttccgc ggttcacggg agagtaccga gcggcattcg tggacttgct ggcgcgcgtg
    11161 ctccgggagg gcccggggcg gggcatcacg gcgctgctca gcgcccagag gatcacgggg
    11221 gagtcccagg ggctcgcaac gctggtgccc gggcggctcc tgctgcgcca tccctcccgc
    11281 caggacttcg tgatagccgg gggagagggt ggccagttcg tcgcggccct tcccgccggg
    11341 cgcggactgt ggcgtggtca gtggatgcag gtggtggccg acccgccgcc cctgccggcg
    11401 accgggccga ctgtcgcgcc gctactggac ccgcggcgcg cgcgagccat cgtgacgagc
    11461 cgggttgcac cattgttggc cagatggccg tcggcaattg cgctctccga cgctggaccc
    11521 gaactccgat cgctggctct tcccggcgtg actatcgtcg gcgatctcga cgagtggcag
    11581 tcacgatggg gagcggtagc ggctctgcgg acccaggccg acatcattct cgacggatgc
    11641 acccccgccg acttccgcgc gatcacgagg tcccggcagc tgcccccgcc ccttgcgccg
    11701 ggtcagtgct ggcaactcaa cgaggacgga tcggcacgcc gagtgcgcct cgacccgccg
    11761 acccgcgact gaacggtacc cctaggctgc gaggatcgcg gcgagttcgg tgtgcttgag
    11821 gccgtgggcc gcggcaaccg gcgcgttcac gatcgaaccc gcatgggaat tgagaccgag
    11881 tgcgagagcc ggatctgcgc gcagggcgtc gagccaacca tggttggcga tcgcccgcac
    11941 gtagggcatg gtcgcattcg tcaacgcgta cgtcgaggtg ttcggcacgg caccgggcat
    12001 gttggcgacg cagtagaaca gcgagccgtg cacagcaaag gtcgggtcgg catgcgtcgt
    12061 cgggcgggtg tcggcaaaac aaccaccctg gtcgacggcg atgtccacga gcacactgcc
    12121 cggcttcatc cgtgagacca ggtcattgct caccagcttc ggcgccttcg ctccgggcac
    12181 gagaaccgaa ccgatcacga gatccgcggt gagcagggca cgttcgatct cgaatccgtt
    12241 cgaggcgacc gtgtgcactc ggcccgcgta gagagcgtcg agttcgcgca ggcgttggat
    12301 gttggtgtcg agcacggtca cctcggctcc caggccgact gcgaccgaga cggcgttcgt
    12361 gcccgccacc ccgccgccga gcacgacaac acgggcgttg tgcgttccgg ggacgccagg
    12421 cacgagaagg ccgggcccgc cattgggctt gagcatcgcg ttcgccccca cgatcggagc
    12481 gaggcgaccc gcgacctcgc tcatcggcgc gagaagcggg agtgctcggt tcggcagttg
    12541 caccgtctcg tacgcgatgg cggtgacgcc agaagttgtg agggctgtgg tcaactcggg
    12601 ctcggcggcg aggtggaggt aggtgaacag cagcaggtcg ctgcggaagt agccgtactc
    12661 cgactcgatc ggctccttga ccttgagcag gagttctgct gcggcccaga ccgtggccgc
    12721 atccggcgcg atggaggcgc cagcggccac gtacgcgtca tcggggatag acgagcccag
    12781 gcctgcacca gtctcgatca gcacctcgtg gccgtgcccg accaggtcgt gcacgcctgc
    12841 cggcgtgatc gcaacacgga actcgttgtt cttgatctct cggggaacgg cgaccttcat
    12901 gcccaacagc gtactgggcg tacaccat
//
LOCUS       DAOSOU010000035        12820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4882_length_12820_cov_6.188092, whole genome shotgun sequence.
ACCESSION   DAOSOU010000035 DAOSOU010000000
VERSION     DAOSOU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12820
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_12820_cov_6.188092"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLW15_03250"
     CDS             <1..461
                     /locus_tag="PLW15_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT33449.1"
                     /translation="SATTRMPRMFGFQAAGSAPMVLGHVVKDPDTIASAIRIGNPASW
                     ELALKAQADSDGYFGSITDDKILEAHRILSAEVGIFVEPASAISVAGLLERAEAGEIP
                     KGSTVVLTVTGHGLKDPQWALRTADGSEVQPTIVSVDTDEVAGVLGLVRA"
     gene            458..1396
                     /gene="thrB"
                     /locus_tag="PLW15_03255"
     CDS             458..1396
                     /gene="thrB"
                     /locus_tag="PLW15_03255"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOT33450.1"
                     /translation="MTDLAGRSVTVRVPATSANLGPGFDTLGLALALYDELTVTATTE
                     PGVHVEVIGVGAGEVPTDETNLVVRSIKHTFDHYGLPMPGLALEAHNVIPHGRGLGSS
                     GAAIVSGIMAAKGLLEGIVDIDSDALLTLATELEGHPDNVAPALFGGLTIAWTTADGP
                     KAKKLIVHRGVSPVVCVPAATMSTALARSLQPASVPHADAVFNVSRSALLIAALIQSP
                     ELLLEATEDKLHQNYRASAMPETDALIQVLRKNGLAAVVSGAGPSVLVLASDPSQRLV
                     AAELVASHATTPWESLMLAVDFKGATVITHQVEATA"
     gene            1554..3596
                     /gene="rho"
                     /locus_tag="PLW15_03260"
     CDS             1554..3596
                     /gene="rho"
                     /locus_tag="PLW15_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT33451.1"
                     /translation="MTDVNIRATGADSRADLSALKLAELQALASSLGIVGATKLRKGD
                     LVNAIEEHDNDNQGVTDAEFEAPQLQTWQPPADEAETPVEPAPEPISEPVAVKKRTSR
                     RVTSADTEAIAAAPVLAGAPEVEVAESAAPSETADAVEVVESAPSRRRSSAAPVARAD
                     FATDDDAQGENPDIETSDSDAPASAGDESGEGEQGSSSRNSRNRNRNRNGGERQGADN
                     GQGAQRQNGQNQNGQSQNGQNQNGQNRNGQNQNGQGGNQQPRLDVDGDNERSGRNRYR
                     DRKRGRGPVGDDFEPEITEDDVLIPVAGILDVLDNYAFVRTSGYLPGNNDVYVSLGQV
                     KKYNLRKGDAVVGAIRQPREGDNNQGRQKYNAIVRIDSINGLPVEEAANRVEFGKLTP
                     LYPQERLRLETEPGKLSTRIIDLVSPIGKGQRGLIVSPPKAGKTLVLQAIANAIAKNN
                     PEVHLMVVLVDERPEEVTDMQRTVKGEVIASTFDRPAEDHTTVAELAIERAKRLVELG
                     HDVVVLLDSITRLGRAYNLAAPASGRILSGGVDSSALYPPKRFFGAARNIEEGGSLTI
                     IATALVETGSKMDEVIFEEFKGTGNMELRLSRQLADKRIFPAVDVNASGTRREEMLMG
                     ADEVKVTWKLRRALAGLDQQQALEIVLGKLKETQSNVEFLMQIQKSMPTAAGAKED"
     gene            3599..4675
                     /gene="prfA"
                     /locus_tag="PLW15_03265"
     CDS             3599..4675
                     /gene="prfA"
                     /locus_tag="PLW15_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT33452.1"
                     /translation="MFESVQQLLAEHDELQAQLADPELHSDPVRSKKVNRRYAELARI
                     VAAYNEWRTLTDDLAAATEMAADDESFAAEIPGLTEALDAAQEKLRRLLIPRDPNDAR
                     DVIMEIKMGEGGAESALFAGDLLRMYLHYAESKKWKTEIIEQTSSDLGGIKDVQLAFK
                     GSSTDPAEGVWAHLKYEGGVHRVQRVPATESQGRIHTSAAGVLVFPEVDEPEEVEINQ
                     NDLKIDVYRSSGPGGQSVNTTDSAVRITHIPTGIVVAMQNEKSQLQNREAGMRVLRAR
                     ILAHYQEIADAEASDYRKSQIRTMDRSERIRTYNFPENRIADHRTGYKAYNLDSVMNG
                     ALEPVIQSCILFDEEGRLADLGSD"
     gene            4710..>4822
                     /locus_tag="PLW15_03270"
     CDS             4710..>4822
                     /locus_tag="PLW15_03270"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33453.1"
                     /translation="MNPNTIAYALDHATAVLEEAGVPSPRVDAELLVGHVLG"
     assembly_gap    4823..4922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4923..5444
                     /locus_tag="PLW15_03275"
     CDS             <4923..5444
                     /locus_tag="PLW15_03275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOT33454.1"
                     /translation="LAASATPQPIGVDLGTGSGAIALAMATEVPHSFVYGVEVSPRAF
                     VWTKQNFRGIDNARAVFIDLAHALSELDGTVDVVISNPPYIPLGAIPRDPEVRLHDPE
                     IALYGGDDGLDVVRQVSATALRLLHAGGTLVLEHGELQAAAIAELLTQDGWRAIAHHR
                     DLLGRDRATTALR"
     gene            complement(5492..6106)
                     /locus_tag="PLW15_03280"
     CDS             complement(5492..6106)
                     /locus_tag="PLW15_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT33455.1"
                     /translation="MSLLFIFDMDDVLYDYDWRLRMSAMTEITGLPLDELRRRWWHND
                     GEWAAEAGGFASAADYLSAFEKAIGMPVDETQFVEARAGAMTVWPESLAAVRRASELG
                     TITLLTNNGPLVSKHLRAIAPELVELFGDHLFTSSDYGARKPDPVVFERVLAAYDATP
                     ENAFFADDMRVNVEGAAGLGITTYHFGTPQGLHAAIEEFAAARA"
     gene            complement(6103..6735)
                     /locus_tag="PLW15_03285"
     CDS             complement(6103..6735)
                     /locus_tag="PLW15_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT33456.1"
                     /translation="MDVYFFDLDKTLYAYDFRRRLPALAVLTGASQYHLARTWWADGY
                     EARAEAGEWPTPAAYLAEFARVTETRRLSLAEWASARAAAMTRIDGSMVALRRASELG
                     VVSLLSNNPAATASALPILAPDVVETLGRNQLFSYMLGARKPGEEIYRRALAHYGVDA
                     ADAFLADDSLANVEGARSVGMTAFQLLRVDGQFQTDALLAAVETFAARSR"
     gene            6782..7438
                     /locus_tag="PLW15_03290"
     CDS             6782..7438
                     /locus_tag="PLW15_03290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT33457.1"
                     /translation="MAPIYDCSVEAELLTGMRLARTAIGRGDLVVLPTDTVYGLAADA
                     FNPAAVQKLIDTKGRTRQSPPPVLIPGIPTLDALAEGVPDEVRALVAKFWPGGLTVVV
                     AANSALAWDLGDTRGTVALRMPDNRIALELLSETGPLAVSSANLTGQPAATTAAEAEA
                     MLGDAVAVYLDGGEAGTVASTIVDATALALPDGKLRIVREGAIPSDDIRALVGADRCE
                     "
     gene            7429..8676
                     /locus_tag="PLW15_03295"
     CDS             7429..8676
                     /locus_tag="PLW15_03295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT33458.1"
                     /translation="MRIVLYALVTGIAAVVTYGLAILIWKLSTKYRLYPKIRERDVHT
                     RPTPRLGGVAMFLGMVVAFGVASQVPELSLVFSDAGKVLGILGAALIIVLIGVADDIW
                     DLDWVTKLAGQIIAAGVLAWQGVQLQTLPIGGQTIVSPYISLIITVLAVVLVMNAINF
                     IDGLDGLVAGVALIANSVFFVYTYLLQLQAQTEYFNLASLTSAVLVGACIGFLPLNFH
                     PAKLFMGDAGALLVGLLMAASAILVTGQVNPEVVDRPDVYTAYFLPAFIPILLPFAVL
                     IVPLLDFGLAVTRRLRAGKSPFTADRKHLHHRLLDMGHSHMHAVLIFYAWTLVVSVGC
                     LGFMFLPFWVAALLILVGLAICTVITLSPLSKRKALEVVAQSTEADDAAIDPEVAKFD
                     GLDAAGVDPDELPLPTTLHKETP"
     gene            8673..9137
                     /locus_tag="PLW15_03300"
     CDS             8673..9137
                     /locus_tag="PLW15_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33459.1"
                     /translation="MTSSVVHRALVFGGIVLAAVAVIGSIVGLLAAGTSGLVSALFAA
                     ALTALFLGMTTVSIVVAERITRTSKSMGVYFGIVIGAWLLKFVVFVVVLLMTRGAEWL
                     NPFVFFYALIAAVLGSLVADVVALAGARVSAIPAQTSPAPDAAAEASANPPA"
     gene            9315..10067
                     /gene="atpB"
                     /locus_tag="PLW15_03305"
     CDS             9315..10067
                     /gene="atpB"
                     /locus_tag="PLW15_03305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOT33460.1"
                     /translation="MDEFFPPAVLFAGTPFELNRIMLIRLLVMAVLIIVFWLGTRNMK
                     LVPTRGQVAFEFITGFVRNNIIVGTLGEKDGKRFMPVLMTIFFVTLGMNITGVIPPLQ
                     IAGTSVIGLPLIMSVVAYVLFIYAGIRKFGFRYFKNALILPGVPIALVPFLAILEFLS
                     TFILRPVTLTLRLLMNMVAGHMLLVLCFAATDFFLFTVLADGNFLGLLGVGTFAFGIA
                     FTLLEIFVAALQAYVITILAAIYIQMALAEEH"
     gene            10114..10335
                     /gene="atpE"
                     /locus_tag="PLW15_03310"
     CDS             10114..10335
                     /gene="atpE"
                     /locus_tag="PLW15_03310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOT33461.1"
                     /translation="MNIVGQLAPLAFGLAAIGSGIGVGLIVGKTIESVARQPELQGRL
                     QGLMFLGIALTEALAFIAIAVAFIPFPAP"
     gene            10364..10912
                     /locus_tag="PLW15_03315"
     CDS             10364..10912
                     /locus_tag="PLW15_03315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOT33462.1"
                     /translation="MLTAFAVKAAEEEAPSLLLPTLPDLIWGTIVFVIILAFFIWRVV
                     PRLNAALDARADAIEGGLKRAEEAQAGAQSALEEYKAQLAEARAEAATIRDAAREDAK
                     RIRAELVEQAQADAARIVTSAQAQIEAERQSALVSLRAEVGAIAIDLASGVVGESLND
                     KRSKDIVDRLLADLETESKAKA"
     gene            10915..11703
                     /locus_tag="PLW15_03320"
     CDS             10915..11703
                     /locus_tag="PLW15_03320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOT33463.1"
                     /translation="MGSATREALSAATATLSEQRTVDLATGEQLLAAALVVDGSAPLR
                     SALSDDTAPVESRRSIVKSVFGSYTPAAQAVLESLASGRWSSEDDFVASIERLGIRAV
                     ASSAPKSVSVDDELFAFSNAVSSNAELELALGSKLGSSEGKVALVTKLLAGKASEQTV
                     AILKALLAQPRGRRIGELVRYASTIVAEAFGHTIATITVATPLAADQTARLEKALSAR
                     YGTTIRINELVDPAVLGGMRVQIGDDVIDGSIATRLTDLRLQLA"
     gene            11746..>12820
                     /gene="atpA"
                     /locus_tag="PLW15_03325"
     CDS             11746..>12820
                     /gene="atpA"
                     /locus_tag="PLW15_03325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOT33464.1"
                     /translation="MAELTISPDEIRDALKEFVKAYEPGKATTTEVGYVVDAADGIAH
                     VEGLPGAMANELLRFADGTLGLAQNLDEDEIGVVILGEFTGIVEGMEVTRTGEVLSVP
                     VGDAFLGRVVDPLGAPIDGLGEIKASTRRALELQAPGVMQRKSVHEPMQTGIKAIDAM
                     IPIGRGQRQLIIGDRQTGKTAIAIDTIINQKANWESGDTNKQVRCIYVAIGQKGSTIA
                     AVKGALEDAGAMEYTTIVAAPASDPAGFKYLAPYTGSAIGQQWMYDGKHVLIIFDDLS
                     KQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGAGSMTGLPIIETK
                     ANDVSAYIPTNVISITDGQIFLQS"
BASE COUNT         2143 a         4501 c         3971 g         2105 t
ORIGIN      
        1 tctcggccac cacccgcatg ccgcgcatgt tcggcttcca ggccgcaggc agcgcaccga
       61 tggtgctcgg ccacgtcgtc aaggaccccg acaccatcgc ctcggctatc cgcatcggta
      121 acccggcgtc gtgggagctc gcactgaagg cgcaggccga cagcgacggg tacttcgggt
      181 cgatcaccga cgacaagatc ctcgaggctc accgcatcct gtccgccgag gtcggaatct
      241 tcgttgagcc cgcgagcgcg atcagcgtag ccggtcttct cgagcgggcc gaggccggcg
      301 agatcccgaa gggatcgacc gtcgttctca cggtgaccgg ccacggactc aaggaccccc
      361 agtgggcact gcgcaccgcc gacggctcgg aggtgcagcc cacgatcgtc tccgtcgaca
      421 ccgatgaggt ggcaggggtt ctcggcctgg tgcgcgcatg accgacctcg ccggtcgctc
      481 tgtcacagta cgagtgcctg cgacatccgc caatctgggt ccgggattcg acaccctcgg
      541 actcgccttg gcgctctacg acgagttgac ggtcaccgcc acaaccgaac cgggtgtgca
      601 cgtcgaggtc atcggcgtcg gcgccggcga ggtgccgacc gacgagacca acctcgtggt
      661 gcgctccatc aagcacacct tcgaccacta tgggctgccc atgcccggtc tcgcactcga
      721 agcccacaac gtgatcccgc acggtcgcgg cctcggctcc tcgggtgccg ccatcgtctc
      781 cggcatcatg gcggccaagg gtctgctcga gggaatcgtc gacatcgatt cggatgccct
      841 gctcacgctc gccaccgagc tcgagggcca ccccgacaat gtcgcgcccg cactcttcgg
      901 cggcctgacg atcgcctgga cgacggcgga cggccccaag gccaagaagc tcatcgtgca
      961 ccgcggagtg tcgccggtcg tctgcgttcc cgcggcgacg atgtcgaccg cgctggcccg
     1021 cagcctgcag ccggcgtcgg ttcctcacgc ggatgccgtc ttcaacgtgt cgcgtagcgc
     1081 ccttctcatt gccgcgctca tccagagtcc cgaactgttg ctcgaagcga ccgaggacaa
     1141 gctgcaccag aactaccggg cgagtgcgat gccggagacg gatgcgctca tccaggttct
     1201 ccgcaagaac gggttggcgg cggtggtatc gggcgccggt ccctcggttc tcgtgctcgc
     1261 cagcgacccg tcccagcgac tcgtcgcggc cgagctggtg gcctcgcacg ccaccacgcc
     1321 gtgggagtcg ctcatgctgg ccgtcgattt caaaggtgct acagtgatca cgcaccaggt
     1381 agaagcaacg gcctagccgc tattcccggt tgcaccatca ccctcgcctc atcgcacaag
     1441 aattcctgtg cgctcgcgcc gtcatcatca cggctcggct cgcagagcgt gtgaggcgat
     1501 cagctctccg catcgagtga ctaactctcg aggtggggaa aggaacccaa gtagtgactg
     1561 acgtcaacat ccgcgccacc ggtgcggatt cccgcgctga cctgagcgcc ctcaagcttg
     1621 ccgagctcca ggctctggcg agttcgctgg gcatcgtcgg cgcaacgaaa cttcgtaaag
     1681 gagacctcgt gaacgcaatc gaagagcacg acaacgacaa ccagggcgtg accgacgccg
     1741 agttcgaagc accgcagctt cagacctggc agccgcccgc tgacgaggcc gagaccccgg
     1801 tcgagccggc ccccgagccg atctccgagc ccgtcgctgt gaagaagcgc acctcgcgtc
     1861 gagtgacgag tgccgacacc gaggccatcg ccgccgcgcc cgtgctggca ggcgcgcccg
     1921 aggtcgaggt cgccgaatcg gccgccccgt ccgagactgc tgacgccgtc gaggtcgtcg
     1981 agtccgcacc cagccgccgc cgttcatcgg ctgcgccggt tgctcgggcc gacttcgcca
     2041 ccgacgacga cgcgcagggc gagaaccccg acatcgagac ctccgactcc gacgcgccgg
     2101 catccgctgg cgacgagtcc ggcgagggcg agcagggaag ctccagccgc aactcgcgca
     2161 accgcaaccg caaccgcaac ggcggagagc gtcagggcgc cgacaacggt cagggcgccc
     2221 agcgccagaa cggccagaac cagaacggtc agagccagaa cggccagaac cagaacggcc
     2281 agaaccgcaa cggccagaac cagaacggcc agggcggcaa ccagcagccc cgcctcgatg
     2341 tcgatggtga caacgagcgc agcggccgca accgctaccg cgaccgcaag cgcggtcgtg
     2401 gcccggtcgg cgacgacttc gagcccgaga tcaccgagga tgacgtgctc atcccggtcg
     2461 ccggcatcct cgacgtgctc gacaactacg cgttcgtgcg cacctccggt tacctgccgg
     2521 gcaacaacga cgtctacgtc tctctcggcc aggtcaagaa gtacaacctg cgcaagggcg
     2581 acgccgtggt cggcgccatc cgccagccgc gcgagggcga caacaaccag ggtcgccaga
     2641 agtacaacgc gatcgtgcgc atcgactcca tcaacggtct gcccgtcgag gaggccgcca
     2701 accgtgtcga gttcggcaag ctgacgccgc tctacccgca ggagcgcctg cgcctggaga
     2761 ccgagcccgg caagctctcg acccgcatca tcgacctcgt gtcgccgatc ggcaagggcc
     2821 agcgcggact catcgtctcg cctcccaagg ctggcaagac gctcgttctg caggccatcg
     2881 ccaacgcgat cgccaagaac aaccccgagg tacacctcat ggttgtgctc gtcgacgagc
     2941 gccccgaaga ggtcaccgac atgcagcgca cggtcaaggg tgaggtcatc gcctccacct
     3001 tcgaccggcc cgccgaagac cacaccacgg tcgccgagct cgccatcgag cgcgccaagc
     3061 gtctggtcga gctcgggcac gacgtggtcg tgctcctcga ctcgatcacc cgtctcggtc
     3121 gtgcctacaa cctggccgct cccgcctcgg ggcgcatcct ctccggaggc gtcgactcgt
     3181 cggccctcta cccgccgaag cgtttcttcg gtgccgctcg caacatcgag gagggcggct
     3241 cgctgaccat catcgcgact gcgctcgtcg agaccgggtc caagatggac gaggtcatct
     3301 tcgaggagtt caagggcacg ggcaacatgg agctgcgcct gtcgcgccag ctggctgaca
     3361 agcggatctt ccccgcggtg gatgtcaacg catccggaac ccgccgtgag gagatgctca
     3421 tgggcgccga cgaggtcaag gtcacctgga agctgcgtcg tgccctcgcg ggactcgacc
     3481 agcagcaggc gctcgagatc gtcctcggca agctcaagga gacgcagtcg aacgtcgagt
     3541 tcctcatgca gatccagaag tcgatgccga ccgccgctgg cgccaaagag gactagccat
     3601 gttcgagtca gtgcaacagt tgctggccga acatgacgag ctccaggctc agcttgctga
     3661 tccggaactc cactcagacc cggtcaggtc gaagaaggtc aaccggcgct acgcggaact
     3721 cgcgcgcatc gtcgccgcgt acaacgagtg gcgcacgctg accgacgacc tcgcggcggc
     3781 caccgagatg gcggccgacg acgagtcgtt cgcggccgag attcccgggc tcaccgaggc
     3841 actggatgcc gcgcaggaga agttgcgccg tctgctcatc ccgcgcgacc ccaacgacgc
     3901 ccgtgacgtg atcatggaga tcaagatggg ggagggtggc gcggagtccg cgctcttcgc
     3961 cggcgatctg ctgcgcatgt acctgcacta cgcggaatcc aagaagtgga agaccgagat
     4021 catcgagcag acctcgagcg atctgggcgg catcaaggat gtccagctcg ccttcaaggg
     4081 gtcgtccacc gacccggcgg agggcgtctg ggcccacctc aagtacgagg gtggcgtgca
     4141 ccgggtgcag cgcgttccgg cgacggagtc gcagggtcgc atccacacgt cggccgccgg
     4201 cgtgctcgtg ttccccgaag tggacgagcc ggaggaggtc gagatcaacc agaacgacct
     4261 caagatcgac gtctaccgct cgagtggccc cggcgggcag tcggtcaata cgaccgactc
     4321 cgccgtgcgc atcacgcaca ttcccacggg aatcgtcgtg gcaatgcaga acgagaagtc
     4381 gcagctgcag aaccgcgagg ccggcatgcg ggtgctgcgc gcgcgaatcc tggcccacta
     4441 tcaggagatc gccgatgctg aggcgagcga ctaccgcaag agccagatcc gcactatgga
     4501 tcgctcggag cgcatccgca cctacaactt ccccgagaac cgtatcgccg accaccgcac
     4561 cgggtacaag gcctacaact tggactccgt catgaacggg gctctcgagc ccgtcatcca
     4621 atcgtgcatc ctcttcgatg aggaaggccg gctcgccgac ctcggctccg actaggccgg
     4681 ttgagtaggg cgaagcccgt atcgaaacca tgaacccgaa caccatcgcc tacgccctcg
     4741 accacgccac agccgtgctc gaggaggccg gagtgccgtc gccccgcgtc gatgccgaac
     4801 tgctcgtcgg ccatgtgctg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnctcgcggc atccgcaacc ccgcaaccga tcggtgtgga tctcggcacc ggcagcggag
     4981 cgatcgccct cgcgatggcc accgaggtgc cgcactcctt tgtctacggc gtggaggtct
     5041 cgccgcgagc cttcgtctgg acgaagcaga acttccgcgg catcgacaac gcgcgtgcgg
     5101 tcttcatcga cctcgcgcac gcgctttccg agctcgacgg cacggtcgac gtcgtcatct
     5161 ccaaccctcc ctacattcct ctgggtgcga ttccgcgcga ccccgaggtg cgcctccacg
     5221 accccgagat cgccctctac ggcggcgacg acgggctgga tgtggtgcgc caggtctcag
     5281 cgaccgccct caggcttctt cacgccggcg gcacccttgt gctcgagcac ggcgaactgc
     5341 aggccgcggc catcgccgag ctgctaacgc aggacggctg gcgcgcgatc gcccaccacc
     5401 gcgacctgct cgggcgagat cgggcgacga cggccctgcg ttagccgggc cgactgccac
     5461 accgtcaggc tcgcgccgca gcgcccacgg gtcaggctcg cgccgcagcg aactcctcga
     5521 tcgccgcgtg cagcccctgc ggcgtgccga agtggtaggt cgtgatcccc aggcccgccg
     5581 cgccctccac attcacacgc atgtcgtcag cgaagaaggc gttctcgggt gtcgcgtcgt
     5641 aggccgcgag cactcgctcg aacaccacgg ggtcgggctt gcgcgctccg tagtcgctcg
     5701 acgtgaacag gtggtcgccg aacagctcga cgagctcggg ggcgatcgcg cgcaggtgct
     5761 tcgacaccag gggaccgttg ttcgtgagca gcgtgatcgt gcccaattcg gatgcccgcc
     5821 gcaccgccgc gagcgactcc ggccacaccg tcatcgcccc ggcacgcgcc tcaacgaatt
     5881 gcgtttcgtc cacgggcatc ccgatggcct tctcgaacgc ggacaggtag tcggccgccg
     5941 acgcgaagcc gccggcctcg gcggcccact cgccgtcgtt gtgccaccag cggcggcgca
     6001 gctcgtcgag cggaaggccg gtgatctcgg tcatggccga catgcgcagg cgccagtcgt
     6061 agtcgtagag cacgtcgtcc atgtcgaaga tgaagagcag gctcatcgcg acctcgcagc
     6121 gaaggtttcg acggccgcca ggagtgcgtc ggtctggaac tgcccgtcga ctcggagcag
     6181 ctggaatgcg gtcatgccca ccgatcgagc gccctcgacg ttggccagcg aatcgtcggc
     6241 aagaaacgcg tcagcggcgt ccacgccgta gtgggcgagc gcgcgacgat agatctcctc
     6301 gcccggcttg cgcgcaccga gcatgtagct gaacagctga ttgcgtccca gggtctcgac
     6361 gacgtcgggg gcgaggatgg gcaaggcgct cgcggtcgcc gcggggttgt tggagagcaa
     6421 cgacacgact ccgagttcgg aggctcgacg caaggcaacc atgctcccat cgattcgggt
     6481 catcgcggca gcccgggcag acgcccactc ggcgaggctc agccgtcggg tctcggtgac
     6541 ccgcgcgaac tcggcgaggt aggcggccgg cgtcggccac tcgcccgcct cggcgcgggc
     6601 ctcgtacccg tcggcccacc acgtgcgtgc gagatggtac tggcttgcgc cggtcagcac
     6661 tgcgagcgcc ggcagacgtc gacggaagtc gtacgcgtac agcgtcttgt cgagatcgaa
     6721 gaagtaaacg tccacgggct ctcaagtctg tcaccgaatc gccgggtacg atgatgggga
     6781 catggccccc atctacgact gctcggtcga ggctgagctc ctcaccggta tgcgcctcgc
     6841 caggacggcg atcggtcgcg gtgatctcgt ggttcttccg accgacaccg tctacgggct
     6901 cgcggccgac gcattcaacc ctgccgctgt gcagaagttg atcgacacca aggggcgcac
     6961 gaggcagtct ccgccgccgg tgctgatccc gggcatcccg actctggatg ctctcgccga
     7021 gggtgtgccc gacgaggttc gcgccctcgt ggcgaagttc tggccgggtg gcctgacggt
     7081 agttgtcgcc gccaactccg cgctcgcctg ggatctcggc gacacccgtg gcaccgtcgc
     7141 cctgcgcatg cccgacaacc gcatcgcgct cgagctgctg agcgagaccg gcccgctggc
     7201 agtgtccagt gccaacctca cgggccagcc cgccgccacc acggccgcgg aggctgaggc
     7261 gatgcttggc gacgccgtcg ccgtctacct cgacggggga gaggcgggaa ccgtcgcctc
     7321 gaccatcgtg gatgccacgg ctctggccct gcccgacggc aagctgcgca tcgtgcgcga
     7381 gggtgccatc ccgtccgacg acatccgtgc cctcgtcgga gcggatcgat gcgaatagtc
     7441 ctctacgcgc tcgtcaccgg catcgccgcc gtcgtgacct acgggctcgc gattctcatc
     7501 tggaagctgt ccaccaagta ccggctgtac ccgaagatcc gtgagcgcga cgttcacacc
     7561 cgcccgactc cgcggctggg tggcgtggcg atgttcctcg gcatggtcgt cgccttcggc
     7621 gtggcatccc aggtgcccga gctcagcctc gtcttctccg acgcgggcaa ggtgctcggc
     7681 atcctcgggg cggctctgat catcgtgctg atcggcgtcg ccgacgacat ctgggatctg
     7741 gactgggtca ccaagctcgc cggccagatc atcgccgcgg gcgtgctcgc ctggcagggc
     7801 gtgcaactgc agaccctgcc gatcggcggc cagacgatcg tctcgcccta catctcgctg
     7861 atcatcaccg tgctcgcggt ggtgctcgtc atgaacgcca tcaacttcat cgacggcctc
     7921 gacggtctcg tcgccggggt cgcgctcatc gccaacagcg tcttcttcgt ctacacctac
     7981 ctgctccagt tgcaggcaca gaccgaatac ttcaacctcg cctcgctcac ctccgcggtg
     8041 ctggtcggcg cgtgcatcgg cttcctgccg ctcaacttcc accccgcaaa gctgttcatg
     8101 ggtgatgccg gtgcactgct cgtcgggctc ctcatggccg cctccgccat cctcgtcacc
     8161 ggtcaggtga accccgaggt ggtggaccgg ccggatgtct acaccgccta cttcctgccc
     8221 gcgttcatcc cgatcctgct gccgttcgcg gtgctcatcg tgccgcttct cgacttcggt
     8281 ctcgcggtca cccgccggct tcgcgcgggc aagtcaccgt tcaccgccga tcgcaagcac
     8341 ctccatcacc ggctgctcga catgggtcac tcccacatgc atgccgtgct gatcttctac
     8401 gcctggaccc tggtcgtctc ggtcggatgc ctcggcttca tgttcctgcc cttctgggtt
     8461 gcagcgctgc tcatcctcgt ggggctcgcc atctgcaccg tgatcacgct gtcgccgctg
     8521 tccaagcgca aggcgctcga ggtggtcgcc cagtccaccg aggcggatga cgctgccatc
     8581 gaccccgagg tcgccaagtt cgacggtctg gacgccgccg gggtcgaccc cgacgaactg
     8641 ccgctgccga caaccctgca caaggagacc ccatgacatc ctcagtcgtc caccgagcgc
     8701 tcgtgttcgg cggcatcgtg ctcgcggccg tcgccgtgat cggctcgatc gtcgggctcc
     8761 tcgcggcagg cacatcggga ctggtcagtg cactgttcgc ggcagccctc accgcgctgt
     8821 tcctcggcat gaccaccgtg agcatcgtgg tcgccgaacg aatcacccgc acgtcgaagt
     8881 cgatgggcgt ctacttcggc atcgtcatcg gggcgtggct gctcaagttc gtcgtgttcg
     8941 tcgtggtcct gctcatgacc cggggtgccg agtggctgaa tccgttcgtg ttcttctacg
     9001 ccctcatcgc cgcggttctc ggctcgctgg tggccgatgt cgtggccctc gctggcgcgc
     9061 gggtgagcgc gattcctgct cagacgagcc ccgcaccgga tgccgcggcc gaagcatccg
     9121 cgaatccgcc cgcctagcac gtcctcggcg gttatcccct cgaaatactt ttgctagggt
     9181 ggagtaaccg ctgtcttgat cgcaccgttt cgaacgcctg ctcccggagt ccccaactga
     9241 ttacctcgac gctcgtctcg cttctcccgc tcgtcaccgg tgacgactcg gacggtggtt
     9301 ttcacggtcc gtccatcgac gagttcttcc ccccggccgt cctcttcgcc gggaccccct
     9361 tcgagctgaa ccgcatcatg ttgatccggc tgctcgtcat ggcggtgctg atcattgtct
     9421 tctggctcgg cacccgcaac atgaagctcg tgccgacccg tggccaggtt gccttcgagt
     9481 tcatcacggg cttcgtgcgc aacaacatca tcgtgggcac cctcggtgag aaggacggca
     9541 agcgcttcat gccggtgctc atgacgatct tcttcgtcac gctgggcatg aacatcaccg
     9601 gcgtcatccc gcccctgcag atcgcgggca cgtcggtcat cggcctgccg ctcatcatgt
     9661 cggtcgtcgc ctacgtgctg ttcatctacg cgggcatccg caagttcggc ttcaggtact
     9721 tcaagaacgc gctgatcctg ccgggtgtgc cgatcgcgct cgtgccgttc ctggcgatcc
     9781 tcgagttcct ctccacgttc atcctgcgcc cagtcaccct gacgctgcga cttctcatga
     9841 acatggtcgc cggtcacatg ctgctcgtgc tctgcttcgc ggctactgac ttcttcctct
     9901 tcaccgttct tgctgacggc aacttcctcg gtctactcgg ggtcggtacc ttcgcgttcg
     9961 gcatcgcctt cacgctgctc gagatcttcg tcgccgcgct gcaggcctac gtcatcacca
    10021 tcctcgccgc catctacatc cagatggcgc tggccgaaga gcactgagcc tcacgctcct
    10081 cggacaagca atcacccctg aaaggaaaca gcaatgaaca ttgttggaca gctcgcaccg
    10141 ctcgccttcg gtctggccgc catcggctcc ggtatcggcg tgggtctcat cgtcggcaag
    10201 accatcgagt cggtggcacg ccagcccgaa ctgcagggcc gcctgcaggg cctgatgttc
    10261 ctcggtatcg cactgaccga ggcgctcgcg ttcatcgcca tcgcggtcgc gttcatcccg
    10321 ttccccgcgc cgtaaccccc acccgccact caaaggagtc cgaatgctca ccgcattcgc
    10381 tgtgaaagcc gctgaagaag aggcacccag cctgctgctg ccgacgctgc ccgacctcat
    10441 ctggggcacg atcgtcttcg tcatcatcct ggcgttcttc atctggaggg tcgtcccgcg
    10501 actgaacgcc gcgctcgacg cgcgcgccga tgctatcgaa ggcggcctca agcgcgccga
    10561 ggaggcgcag gcgggagccc agtcggcact cgaggagtac aaggctcagc tggccgaggc
    10621 ccgtgccgag gctgcgacga tccgcgatgc cgcccgcgag gacgcgaagc gcatccgcgc
    10681 cgagctcgtc gagcaggctc aggctgatgc cgcgcgcatc gtcacgagcg ctcaggctca
    10741 gatcgaggcg gagcgtcaga gcgctctcgt atcgctgcgc gccgaggtgg gcgcgatcgc
    10801 gatcgatctc gcgtcgggcg tcgtgggcga gagcctcaac gacaagcggt cgaaggacat
    10861 cgtcgaccgc ctgctggctg acctcgagac cgagtcgaag gccaaggcgt aaccatgggt
    10921 tcagcgacac gagaggcact cagcgcggca accgccacgc tgagcgagca gcgcacggtc
    10981 gatctcgcca ccggcgagca gcttctggcg gccgctcttg tcgtcgacgg atcggcgccg
    11041 ttgcgctcgg ccctgtccga tgacacggca ccggtggaga gccgtcgctc catcgtgaag
    11101 tcggtgttcg gctcctacac tcccgcggcg caggccgtgc tggagtcgct cgcgagcgga
    11161 cgctggtcga gtgaggatga cttcgtcgca agcatcgagc gcctgggcat ccgtgccgtc
    11221 gcgtcatccg caccgaagtc ggtgtcggtc gacgacgagc tgttcgcgtt ctcgaacgcc
    11281 gtctcgtcga acgccgagct cgagctcgct ctgggcagca agctcggatc atccgagggc
    11341 aaggtggccc tggtcacgaa gctgctcgca ggcaaggcga gcgagcagac tgtcgcgatc
    11401 ctgaaggcac ttctcgcgca gccccggggt cgccgcatcg gcgaactcgt gcggtacgcg
    11461 tcgacaatcg tcgccgaggc cttcggtcac acgattgcga ccatcacggt cgccacgcct
    11521 ctggccgccg accagactgc acgcctcgag aaggcgctgt ctgctcgata cggcaccaca
    11581 atccgcatca acgaactcgt tgaccccgcc gttcttggcg gcatgcgtgt gcagatcggt
    11641 gacgacgtca tcgacggcag catcgcgacc cgactcaccg acctcaggct tcagctcgcc
    11701 tagcgcgagt ggcacgagaa gtttccacga aagaggaatg aaccaatggc agaactgacc
    11761 atcagcccgg acgagatccg cgacgcgctc aaggagttcg tcaaggctta cgagcccggc
    11821 aaggcgacga cgaccgaggt cggctacgtc gtggatgccg cagacggcat cgcccacgtc
    11881 gagggcctgc ccggcgccat ggccaacgag ctcctgcgct tcgccgacgg caccctgggc
    11941 ctggcccaga acctcgacga ggacgagatc ggtgtcgtca tcctcggtga gttcacgggc
    12001 atcgtcgagg gcatggaggt gacccgcacc ggcgaggtcc tgtccgtccc cgtcggtgac
    12061 gcgttcctcg gtcgcgtcgt cgacccgctc ggcgctccca tcgacggtct cggcgagatc
    12121 aaggcatcca ctcgtcgtgc cctcgagctc caggcgcccg gcgtcatgca gcgcaagagc
    12181 gtgcacgagc ccatgcagac cggcatcaag gccatcgacg cgatgatccc gatcggtcgc
    12241 ggccagcgcc agctcatcat cggcgaccgc cagaccggta agacggccat cgcgatcgac
    12301 acgatcatca accagaaggc caactgggag tcgggcgaca ccaacaagca ggtgcgctgc
    12361 atctacgtcg ccatcggaca gaagggctcc acgatcgcgg ccgtcaaggg cgcgctcgag
    12421 gatgcgggcg ccatggagta cacgacgatc gtcgcggccc cggcttccga ccctgccggc
    12481 ttcaagtacc tcgcgccgta caccggttcg gcaatcggcc agcagtggat gtacgacggc
    12541 aagcacgtcc tcatcatctt cgatgacctg tccaagcagg ccgaggccta ccgcgccgtg
    12601 tcgctgctgc tgcgtcgccc gccgggacgt gaggcctacc ccggtgacgt gttctacctg
    12661 cactcccgtc tgctggagcg ttgcgccaag ctctccgacg agctgggtgc cggatcgatg
    12721 accggacttc cgatcatcga gaccaaggcc aacgacgtct ccgcctacat tccgaccaac
    12781 gtgatctcga tcaccgacgg ccagatcttc ctccagtccg
//
LOCUS       DAOSOU010000036        12769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4904_length_12769_cov_5.905930, whole genome shotgun sequence.
ACCESSION   DAOSOU010000036 DAOSOU010000000
VERSION     DAOSOU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4904_length_12769_cov_5.905930"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PLW15_03330"
     CDS             <1..1031
                     /locus_tag="PLW15_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HOT33465.1"
                     /translation="ALLQPHGLALANMGDIDRQTISGATSTGTHGTGSAFGGLATQIR
                     AVTLVTAAGEVLKVSETENSELLPAAVLGLGALGVVVDLTIECVPTFVLHAVERPEPL
                     ETVLAEWSERIAKNDHFEFYVWPHTETALTKTNTRLPGDAPRHPLGAFSGWFDDEFMA
                     NGLYRGIVNLGHLAPGTIPPINRLAVKLSGNREFTDFSPNVFTTNRTVRFKEMEYAIP
                     VEQVPAAVAEVRALIADRGWNISFPIEVRASAADDLMLSTASGRATGYIAVHRFFRED
                     EREYFGAVESIMRAHDGRPHWGKMHRRTADDLRPAYPRFDEFVAVRDRLDPDRLFANE
                     YLTRVLGE"
     gene            1028..2452
                     /locus_tag="PLW15_03335"
     CDS             1028..2452
                     /locus_tag="PLW15_03335"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOT33466.1"
                     /translation="MSDYRSEWLHDPAGLAAQMPAGFTMGVATAAFQVEGAVREDGRG
                     PSTWDTFMAQPGRIADGSDASVTDDHYHRYREDVALMKELGVDAYRFSLAWPRIQPGG
                     TGPANKAGIAFYDRLLDELLAAGISPVATLFHWDMPEPLQHKGGWMTRDTASRFADYA
                     LLAAQAFGDRVDKWITLNEPTSVTLNGYALGVHAPGASLMFDSLPAVHHQLLGHGLAV
                     QALRSENVTGGIGITNIHSPVVPAKDNFTTRGYAEVFDLVHNRIFADPVLLGRYPKFP
                     FLARKLFKPLIEVDSDDLKTIHQPLDFYGLNYYAPTRIASGPPADDRSPDGTPAPGLR
                     GLPFHLASFPEFPTTGFGWSIAPEYLEVTLKDYAERYAGVLPPVYITEGGASFDDVVA
                     ADGSVDDADRASYLAEHLAVAFASVPGLDLRGYFAWTLMDNWEWAAGFGERFGLVRVD
                     FDTLERTPKRSYRWLQQLIASRSS"
     gene            2479..3045
                     /locus_tag="PLW15_03340"
     CDS             2479..3045
                     /locus_tag="PLW15_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT33467.1"
                     /translation="MEILIVIGVIVLLVVIVGIYLWSTYNGLVTLNVRVDEAWSDITV
                     QLKRRADLIPNLIEAVKGYAAHEKAVFENVTKARAETLSAQGPVDAAAAENHMQTALK
                     SIFAVAEAYPQLQANQNYLQLQGELVDTEDKIQASRRFYNGGVRELNTKIKVFPNTLF
                     VRGLGFTEREFFEVSDSAAIAEPPRVQF"
     gene            3110..3991
                     /locus_tag="PLW15_03345"
     CDS             3110..3991
                     /locus_tag="PLW15_03345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOT33468.1"
                     /translation="MYRAIAKNKRNTVFIILLFILIIGGLAALAAYIYRDWTIVVITL
                     VIATGYAVIQYFAASRQALSLSGAREIQKADNPRLYRIVENLAITTGIPMPKVYIIND
                     PAPNAFATGRDPQHASVAATTGILELLDDSELEGVMAHEIGHVQNYDIRVSMIVFGLV
                     VAVGFIADMLLRIAFFGGRGNNNGGGNPIVLVFGLAAFIVAPLVAAIVQAAISRQREY
                     LADATGAFTTRHPDALASALEKLGQYGRPMQRQNSSMAHMWISDPSKPGAMARLFSTH
                     PPIAERIARLGKIGSSF"
     gene            complement(4041..5153)
                     /locus_tag="PLW15_03350"
     CDS             complement(4041..5153)
                     /locus_tag="PLW15_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33469.1"
                     /translation="MNSLRSSGMLLVLTTALALTACTPPEAGPTPAASSPSATPTPSP
                     TVAAISAPAPVFDVECGDLASEATLADALGASAISIDPAEATMGAYPAISPTYAVRSL
                     GGITCEWSNGEPQSDTAGLNPAYVGTRVLVLPNATSQWERYLAYYGPDAEGDYCGAYE
                     GPLQCTTDVLTGSTWVQLTTVGATSEAAASALGAEVVAAISGAGPGAAPWTPPAGTSA
                     LPADCPGVIPDAAVQSALGIDATVQASSGGGGWSLEAGARENWGGPHCYWAFLDSDAG
                     VGSLATLPGGAWAWDEARTFLTLPSVPEAVTIAGLSADDEAWLRCAPGDAYCLIDLVI
                     GGNWVEAYVWPDDAGFSIDRRAGALAIGEAIVASLG"
     gene            complement(5150..6367)
                     /locus_tag="PLW15_03355"
     CDS             complement(5150..6367)
                     /locus_tag="PLW15_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOT33470.1"
                     /translation="MPDSLTPAQARRVALAAQGFGRPIAPVGTRQLNLLLQRLAVLQL
                     DSVNVFERSHYLPVFARLGAYDKALLDRLTFTRRSSYTEYWAHQAAVIPIEQRPLWHW
                     SMAEWRSERKELGRWGAAHPEMLDFLRAELREKGALTSGQVEHDANVRRGPWWGWSDV
                     KMGLETLFFWGEVAVAGRTRFERTYDLAERVVPAEHLGRDIPKQEAIRELVSTASRAL
                     GIATVSDLADYFRLLQAPVRTAVGELTESGELIRVAVDGWREPAYLHRDARLPRRIDA
                     AALLSPFDPVVWHRQRTERMFGFRYRIEIYTPAPKRIYGYYTLPVLIDDQLVARIDLK
                     TDRQAGVLRVQSAWHEPGQHADIERIAQLLRETAAWQGMDRIEVTGVGDLAAALSSQF
                     SSTRPGASNVERA"
     gene            6536..7648
                     /locus_tag="PLW15_03360"
     CDS             6536..7648
                     /locus_tag="PLW15_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33471.1"
                     /translation="MFSKRTLVGAALAATVAISLTACSASGTPAGDVDAATATSAEAF
                     GGLDALIAAAKAEGQLNVIALPDNWANYGEIKALFEETYGITVNSADPDISSAEEIAA
                     ADNLKGQDTAPDVFDLGTAVTLDSLDYFAPYQVANWSEIPDANKEATGLWVNDYTGSM
                     SIGYDSNSVPEPTSLDDLLKPEYKGAVALNGDPTQAGAAFAAVGLATVQSGGTLDDFT
                     PGIDFFGKLNDAGNFIPVNATGATIASGETKVVFDWSYNNFAAAAQVDGWKVTTLPGA
                     VYTSFYNQAVSKDAPHPAAARLWQEFLYSPAAQNLFLVGGAYPVTLQAMVDAGTVDKA
                     ALDALGALDGDLVSVTADQATANAAILAEKWAAAIS"
     gene            7664..8575
                     /locus_tag="PLW15_03365"
     CDS             7664..8575
                     /locus_tag="PLW15_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT33472.1"
                     /translation="MTVATAPVLTTGDAGTPSRRRPLGLVKYLGLTPFAAYVLIFLAI
                     PTTLAVLTGFVTEDGQFTWDNVLGLFQPNVLAAFGASFWVSAVTAVIGAVIGALVCYA
                     MLGTRAEGALRTMVNSASSVLAQFGGVMLAFAFIATIGVQGLVTTILKNQFGVDIFAN
                     GLWFYQVPGLLLPYVYFQVPLMVITFMPALEGLKPQWAEANATLGGTRLTYWRRIAIP
                     VLAPSFLGSLLLLFANAFSSYATAAALISQGSQIVPLQIRGALVSETVLGRENMAGAL
                     ALGMIVVMVVVMTGYSALQTRASRWQR"
     gene            8572..9390
                     /locus_tag="PLW15_03370"
     CDS             8572..9390
                     /locus_tag="PLW15_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT33473.1"
                     /translation="MSNATRIGTEPSKTARWIILVIVGLVFAIPIISMIEFTFRVGPA
                     GGYTFDHWTALFDPANARNYRDLFQSIGNSFILAAVTVAIVLVVLLPTMILVHLQFPR
                     LRRVLEFICIVPITVPAIVLVVGLAPVYSVVVKVFGGSIWTLAFAYGITVLPYAYRSI
                     QANLDAVPVVTLSEAARSLGAGWLSVLGRVLLPNLRRGVLAAAFISVAVVLGEFTIAS
                     LLNRRNLQTALLQVSQSDPWVAVILALLSLLFAFVLLLLIGRLGSLGRGKETTP"
     assembly_gap    9404..9503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9504..10328
                     /locus_tag="PLW15_03375"
     CDS             <9504..10328
                     /locus_tag="PLW15_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33474.1"
                     /translation="ASVPTNKRDMGMVFQSYSLFPHMTAIENVEFGLRMRKVATAERR
                     TRALEALEMVGLTTFAERFAHQLSGGQQQRVALARALVTRPRVLLLDEPLSALDAKVR
                     VQLRDEIRRIQTELGITTVFVTHDQEEALAVADRVAVMRDGVIEQIGTPEELYTAPRT
                     PFVANFVGLSNRLAGEHSKGTVTVQGTRLDLLGDPLPDGPVTAYVRPEDITFAARGIA
                     ATVVSSSFLGSLRRTQVRLADDTLLSIQHDVETRPQPGELVHLKLRGVPVSAVVRS"
     gene            10358..11500
                     /locus_tag="PLW15_03380"
     CDS             10358..11500
                     /locus_tag="PLW15_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT33475.1"
                     /translation="MALGRRKTVAAPPADEAVPPALKVAAAYSWRILVIVGVIAVLVF
                     VIMQFRDIVVPFFVAVLLAALLVPFMNFLVRKGWPRWLAIVVAMVGTLAVIAGLIVLV
                     VLQVRAGYPDLQAQSVEAYDNLKAWLLASPLQLTEADISAYLGQAWEAIQRDSSALVS
                     GLMSVGTTAGHVLAGFLLTLFATLFFLIDGKGVWRWIVGLFPRRSRPAVDGAGQAGWI
                     TLTTFVKVQIFVAAVDAIGIGAGAWILGLFFGGFPLVIPIAVAVFLGSFIPVVGALIS
                     GALAVFVALVYLGPIPAVMMLGIVLLVQQVEGHILQPLVMGTAVKVHPLAVVFAVAAG
                     GFIAGIPGALFAVPVIAVLNVVVKYIAAGEWRTHPHPTVKDLLGDA"
     gene            11493..12731
                     /gene="ilvA"
                     /locus_tag="PLW15_03385"
     CDS             11493..12731
                     /gene="ilvA"
                     /locus_tag="PLW15_03385"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOT33476.1"
                     /translation="MPETAVVGPTLGGFEEARDRVTGVVQVTLMDSSRYLSDILGSPV
                     LLKCENLQRTGSYKIRGAYNRLSLLTDEEKARGVVAASAGNHAQGVAYAARELGIKAT
                     IFMPVGVPLPKLQATKDYGAEVVLRGHTVDEPLRAAADFARTTGAVFIHPFDHEDIIA
                     GQGTLGLEIYEQQPDVDTVVVPIGGGGLISGVASAIKQRAAIDGRTVRVIGVQAANAS
                     PYPVSLEKGAPTQITILPTIADGIAVSKPGELNFEIVRRVVDEVVTVSEDDMAQALLV
                     LLERAKLVVEPAGAVGVAAILAGQVSASGTTVVILSGGNIDPMIMERVISHGLAASER
                     YLKLRIPLPDRPGQLARVSALVAECSANVVEVLHTRHGGGLLISQVELELHIETRGPE
                     HAELVLERLRDEGFEPRIDF"
BASE COUNT         1961 a         4506 c         4076 g         2126 t
ORIGIN      
        1 ccgcgctgct gcagccgcac ggcctcgccc tcgccaacat gggcgacatc gatcgccaga
       61 cgatctccgg cgcgacctcc acgggcacgc acggcaccgg atctgcgttc ggcggtctgg
      121 ctacgcagat ccgcgcggtc acgctcgtca ccgccgccgg tgaggtgctc aaggtcagtg
      181 agaccgagaa ctccgagctg ctgccggcgg ccgtgctcgg tctcggcgcg ctcggcgtgg
      241 tcgtcgacct caccatcgag tgcgtgccca ccttcgtgct gcacgccgtg gaacggcccg
      301 agccgctcga gacagtgctc gccgagtggt cggagcgcat agcgaagaat gaccacttcg
      361 agttctacgt gtggccccac accgagaccg cgctgacgaa gaccaacacc cgtctgccgg
      421 gggatgcgcc gcggcatccg ctcggggcat tctccggatg gttcgacgac gagttcatgg
      481 ccaacggcct gtaccgcggc atcgtgaacc tcggccatct cgcgccgggc acgatcccgc
      541 ccatcaaccg cctcgcggtc aagctctcgg gcaatcgcga gttcaccgac ttctccccga
      601 acgtcttcac caccaaccgc accgtgcggt tcaaggagat ggagtacgcg atccccgtcg
      661 agcaggtgcc cgcagccgtc gcagaggtgc gagcgctgat cgccgaccgc ggctggaaca
      721 tctccttccc gatcgaggtg cgggcgtcgg ccgcagacga cctcatgctc tcgacggcga
      781 gcggccgtgc caccggctac atcgccgtgc accggttctt ccgcgaagac gagcgggagt
      841 acttcggcgc cgtcgagtcg atcatgcgtg cccacgacgg ccgcccgcac tggggcaaga
      901 tgcaccgccg caccgccgac gacctgcgcc ccgcctaccc gcgcttcgac gagttcgtgg
      961 cggtgcgcga ccggctcgac cccgaccgtc tgttcgccaa cgagtacctc acccgggtgc
     1021 tcggggaatg agcgactacc gcagcgagtg gctgcacgac ccggcgggcc tcgccgcgca
     1081 gatgcccgcg ggcttcacga tgggcgtggc gacggccgcc ttccaggtcg agggcgccgt
     1141 gcgcgaagac ggccgcggtc cgtcgacgtg ggacaccttc atggcccagc ccggccgcat
     1201 cgccgacggg tccgatgcct cggtgaccga cgaccactac caccgctacc gcgaagacgt
     1261 cgcgctcatg aaggagctgg gcgtcgacgc gtaccggttc tcactggcgt ggccgcgcat
     1321 ccagcccggc ggcacggggc cagccaacaa ggccggcatc gcgttctacg atcgcctgct
     1381 cgacgaactg ctcgccgcgg gcatcagccc ggtcgcgacg ctgttccact gggacatgcc
     1441 agaacccctc cagcacaagg gcggctggat gacgcgcgac acagcatcgc gcttcgccga
     1501 ctacgcgctg ctcgcggcgc aggccttcgg tgaccgcgtc gacaagtgga tcaccctcaa
     1561 cgaacccacg agcgtcaccc tcaacggcta cgccctcggc gtgcacgcgc cgggcgcgtc
     1621 gctcatgttc gactcgctgc ccgcggtgca ccaccagctg ctcggccacg gcctcgcggt
     1681 gcaggcgctg cgctcggaga acgtgacggg cggcatcggc atcaccaaca tccactcgcc
     1741 ggtggtgccc gccaaggaca acttcacgac ccgcggctac gccgaggtgt tcgacctggt
     1801 gcacaaccgc atcttcgccg acccggtgct gctcggccgc tacccgaagt tcccgttcct
     1861 cgcgcgcaag ctgttcaagc cgctcatcga ggtcgactcc gacgatctga agacgatcca
     1921 ccagccgctc gacttctacg ggctcaacta ctacgcgccc acccgtatcg cgtccggtcc
     1981 gccggccgat gaccgtagcc ccgatggcac cccggcaccc ggccttcgcg gcctgccgtt
     2041 ccatctggct tcgttccccg agttcccgac caccggcttc ggctggtcga tcgcgcccga
     2101 gtacctggag gtcaccctca aggactacgc cgagcgctac gccggtgtgc tgccacccgt
     2161 ctacatcacc gagggtggcg cgagcttcga cgatgtggtc gccgccgacg gctcggtgga
     2221 tgacgctgac cgagccagtt acctcgccga gcacctcgcc gtcgccttcg cgtcggtgcc
     2281 cggtctcgac ctgcgcgggt acttcgcctg gacgctcatg gacaactggg agtgggccgc
     2341 cggcttcggc gagagattcg gtttggtgcg agtcgacttc gacaccctcg aacgcacccc
     2401 gaagcggtca taccgctggc ttcagcagct gatcgcatcc cgttcctcct gacataaagc
     2461 tcccctatag ttgccggtat ggaaatcctc atcgtcatcg gcgtcatcgt cctgctcgtc
     2521 gtcattgtcg ggatctacct ctggtcgacc tacaacggtc tcgtcacgct caacgtgcgc
     2581 gtcgacgagg cgtggagcga catcacggtg cagctcaagc gccgcgcgga cctcatcccg
     2641 aacctcatcg aggccgtcaa gggctacgcg gcccacgaga aggccgtctt cgagaacgtc
     2701 acgaaggcgc gcgccgagac gctcagtgcc caaggccccg tggatgccgc ggccgccgag
     2761 aaccacatgc agaccgccct caagtcgatc ttcgcggtcg ccgaggcgta cccgcagctt
     2821 caggccaacc agaactacct gcagctgcag ggtgagctgg tcgacaccga ggacaagatc
     2881 caggccagcc gtcgcttcta caacggtggc gtgcgcgagc tgaacacgaa gatcaaggtg
     2941 ttcccgaaca cgctcttcgt gcgcggcctc ggcttcaccg agcgcgagtt cttcgaggtc
     3001 agcgactccg cagctattgc agagccgccc cgcgtacaat tctgatcccc gctggttgag
     3061 tagcggcaca gccgcgtatc gaaaccgagt acctggagcc aggagctaga tgtaccgcgc
     3121 catagcgaag aacaagcgca acacggtgtt catcatcctg ttgttcatcc tgatcatcgg
     3181 tggtctcgcc gcgctcgcgg cgtacatcta ccgtgactgg acgatcgtcg tgatcaccct
     3241 cgtgatcgcg accggctacg cggtgatcca gtacttcgcc gccagccgac aggcgctgtc
     3301 gctgagcggt gcgcgcgaga tccagaaggc cgacaacccg cggctgtacc gcatcgtcga
     3361 gaatctggcg atcaccacgg gcatcccgat gccgaaggtg tacatcatca acgacccggc
     3421 gccgaacgcg ttcgcgaccg ggcgtgaccc gcagcacgcg tcggtggcgg cgaccacggg
     3481 catcctcgag ctgctggatg actcggagct cgagggcgtc atggcccacg agatcgggca
     3541 cgtgcagaac tacgacatcc gcgtctcgat gattgtcttc ggcctcgtcg tggcggtggg
     3601 cttcatcgcc gacatgctgc tgcgcatcgc gttcttcgga ggccgcggca acaacaacgg
     3661 cggcggcaac ccgatcgttc tggtcttcgg tctcgcggcc ttcatcgtcg cgccgctcgt
     3721 cgcggcgatc gtgcaggcgg ccatctcccg ccagcgcgag tacctggcgg atgcgacggg
     3781 cgcgttcacc actcgccacc cggatgccct tgccagcgcc ctcgagaagc tcgggcagta
     3841 cggccgtccg atgcagcgtc agaactcgtc gatggcccac atgtggatct cggacccgtc
     3901 gaagcccggc gcgatggcac gtctgttctc gacgcacccg ccgatcgccg aacgcatcgc
     3961 gcgactcggc aagatcggca gcagcttcta gcaaccgctg gttgagtagg ccgtaggccg
     4021 tatcgaaacc gctcgacgac ctacccgagg ctggcgacga tggcctcgcc gatcgccagg
     4081 gcgccggcgc ggcggtcgat ggagaacccc gcgtcgtcgg gccacacgta tgcttcgacc
     4141 cagtttccgc cgatgacgag gtcgatgagg cagtaggcat ccccgggcgc gcatcgcagc
     4201 cacgcctcgt cgtcggccga cagaccggcg atcgtgaccg cctccggcac ggatggaaga
     4261 gtcaggaatg tgcgggcctc atcccacgcc cacgctccac caggaagagt agcgagggat
     4321 cccacacccg cgtccgagtc gaggaaggcc cagtagcagt gcggcccgcc ccagttctca
     4381 cgagcaccgg cctcgaggct ccagccgccg ccaccgctcg acgcctgcac cgtggcgtcg
     4441 ataccgagcg ctgactgcac ggcggcgtcg gggatgacac cggggcagtc ggcaggcagc
     4501 gccgacgtcc cggcgggcgg ggtccagggt gcggcaccgg gcccggcgcc ggagattgcc
     4561 gcgacaacct cggcaccgag cgccgaagcc gcggcctcgg aggtcgcgcc taccgtggtc
     4621 aactgaaccc aggtcgagcc cgtgagcacg tcggtcgtgc attggagtgg tccctcgtac
     4681 gcgccgcagt agtcgccctc ggcatcaggt ccgtagtagg cgaggtagcg ctcccactgg
     4741 ctcgttgcgt tgggtagcac gagtacgcgg gtgcccacgt acgcggggtt taggccagcg
     4801 gtgtcgctct gcggctcccc gttgctccac tcgcaggtga tgccacccag gctgcgcacg
     4861 gcgtaggtgg gcgagatcgc ggggtaggcc cccatagtcg cctcggccgg gtcgatggat
     4921 atcgcgctcg ctccgagcgc gtcggccagc gtcgcctcgc tcgccaggtc gccgcactcc
     4981 acgtcgaaga cgggcgccgg agcggagatc gctgcaacgg tcgggctggg cgtcggtgtc
     5041 gccgacgggg acgaggctgc cggcgttggg ccggcttcgg gaggcgtgca ggcagtgagc
     5101 gccagagcag tggtgagcac gaggagcatg ccagaggagc ggaggctgtt catgctctct
     5161 cgacgttcga ggcacccggt cgggttgacg agaattgcga agaaagtgca gccgcgaggt
     5221 cgccgactcc ggtgacctcg atgcggtcca tgccctgcca cgctgccgtc tcgcgcagca
     5281 gctgcgcgat gcgctcgatg tccgcatgct gcccgggctc gtgccacgcc gactgcaccc
     5341 gcagcacacc ggcctgccgg tcggtcttga ggtcgatgcg ggcgaccagc tggtcgtcga
     5401 tgagcaccgg aagcgtgtag tagccgtaga tgcgcttcgg ggcgggcgtg tagatctcga
     5461 tgcggtagcg gaacccgaac atgcgctcgg tccgctgccg gtgccatacg acggggtcga
     5521 agggggagag cagcgctgct gcatcgatgc gacgggggag ccgggcatcc cgatgcaggt
     5581 aggcaggctc tcgccagccg tcgacagcga cccggatcag ctcgccggac tcggtgagct
     5641 caccgacggc ggtgcgcacg ggcgcctgga gcaggcggaa gtagtcagcg aggtcgctga
     5701 cggtcgcgat tccgagtgct cgtgaggccg tcgagacgag ttcgcggatc gcctcctgct
     5761 tcgggatgtc gcggcccaag tgctccgccg gtaccacccg ctcggccaga tcgtacgtgc
     5821 gctcgaaccg ggtgcgaccg gcgaccgcga cctcgcccca gaagaacagg gtctcgagtc
     5881 ccatcttcac gtctgaccac ccccaccacg gcccgcggcg cacgttcgca tcgtgctcta
     5941 cctgcccgga ggtcagcgca cccttctcgc gcagctctgc ccgcaggaag tcgagcatct
     6001 cgggatgagc ggcaccccag cgccccagct ccttgcgctc actgcgccac tcggccatcg
     6061 accagtgcca gagcgggcgc tgctcgattg ggatgaccgc ggcctggtga gcccagtact
     6121 cggtgtagct gcttcgcctg gtgaaggtga gccggtcgag cagagccttg tcgtacgcgc
     6181 ccagccgcgc gaagaccggc aggtagtgac tgcgttcgaa cacgttcacc gagtcgagct
     6241 ggagcactgc gagccgttgc agcagcaggt tcagctggcg ggtgcccacg ggggcgatgg
     6301 gccggccgaa gccctgagcc gcgagggcga ctcgtcgggc ctgcgcggga gtcagggagt
     6361 cgggcacgag ctcgacacta tcgccgagca ccgacggtca tccgtcgttc gccctgcgtt
     6421 caccgcgggg ttgatccgcc tttcggctcg ctgcttagcg tgggcctcag gccagtcagg
     6481 ctcccttgcc cgctcacagc gcgggcggcc gacggcccag aagaagagga aacccttgtt
     6541 cagtaagcgc actctcgtcg gcgcggcgct cgccgccacc gtcgcgatct ccctcaccgc
     6601 gtgcagcgca tccggcaccc ccgccggtga cgtcgacgcg gccaccgcca cgagcgccga
     6661 ggcgttcggt ggcctcgacg ccctcatcgc cgcagccaag gcggagggtc agctcaacgt
     6721 catcgccctg cccgacaact gggccaacta cggcgagatc aaggccctct tcgaggagac
     6781 ctacggcatc accgtgaact cggccgaccc cgacatctcc agcgccgagg agatcgcggc
     6841 cgccgacaac ctcaagggcc aggacaccgc tcccgacgtc ttcgacctcg gcacggccgt
     6901 caccctcgac agcctcgact acttcgcgcc gtaccaggtc gcgaactggt cggagatccc
     6961 ggacgccaac aaggaggcca ccggcctctg ggtgaacgac tacaccggca gcatgtcgat
     7021 cggctacgac tcgaactcgg ttcccgagcc cacgagcctc gacgacctgc tcaagcccga
     7081 gtacaagggc gctgtcgcgc tcaacggtga cccgacgcag gcgggcgctg ccttcgcggc
     7141 cgtcggtctc gccacggtgc agtccggtgg aacgctggat gacttcaccc ccggcatcga
     7201 cttcttcgga aagctcaacg acgcgggcaa cttcatcccg gtgaacgcga ccggcgcgac
     7261 gatcgcctcg ggtgagacca aggtcgtctt cgactggagc tacaacaact tcgcggccgc
     7321 cgctcaggtg gacggctgga aggtgaccac cctcccgggt gccgtgtaca cgagcttcta
     7381 caaccaggcc gtcagcaagg atgccccgca cccggccgct gcacgcctct ggcaggagtt
     7441 cctctacagc cccgccgcgc agaacctgtt cctcgttggc ggcgcctacc cggtgaccct
     7501 tcaggccatg gtggatgcag gcacggtcga caaggctgcg ctcgatgcac tcggcgccct
     7561 cgacggtgac ctcgtctccg tcaccgcgga ccaggccacg gccaacgccg cgatcctcgc
     7621 cgagaagtgg gctgctgcga tcagctagca gcacctctca ctgatgactg tcgccaccgc
     7681 gccagtactc accacgggcg acgccgggac accctcccgg cgtcgccccc tgggtctggt
     7741 caaatatctc ggactcacgc cgttcgccgc ctacgttctg atcttcctcg cgatccccac
     7801 aacactcgcc gtgctcaccg gatttgtcac ggaggacggt cagttcacct gggacaacgt
     7861 gctgggcctg ttccagccca acgtgctcgc cgcgttcgga gcatccttct gggtgtctgc
     7921 cgtcacggcc gtgatcggcg ccgtcatcgg tgccctcgtc tgctacgcca tgctcggcac
     7981 gcgcgccgag ggtgcactgc gcacgatggt caactccgcc tcgagcgtgc tcgcccagtt
     8041 cggtggcgtc atgctcgcct tcgccttcat cgcgacgatc ggcgttcagg gcctcgtcac
     8101 cacgattctc aagaaccagt tcggcgtcga catcttcgcg aacggactgt ggttctacca
     8161 ggtacccggc ctgctgctgc cctacgtcta cttccaggtg ccgctcatgg tcatcacctt
     8221 catgccggcg cttgaaggcc tgaagcccca gtgggctgag gccaacgcga ccctcggcgg
     8281 cacccggctg acctactggc gtcgcatcgc catcccggtg ctcgcaccgt cgttcctcgg
     8341 cagcctgctg ctgctgttcg cgaacgcctt ctcctcgtac gccactgccg cagctctcat
     8401 cagccagggc tcgcagatcg ttcccctcca gatccgcggc gcgctcgtga gcgagaccgt
     8461 gctcggtcgc gagaacatgg ccggtgcgct ggccctcggc atgatcgtgg tcatggtcgt
     8521 cgtcatgacc ggctactcgg ctctgcagac ccgcgcatcg aggtggcaga gatgagcaac
     8581 gcgacccgca tcggcaccga gccgagcaag acggcccgct ggatcatcct ggtcatcgtc
     8641 ggtctggtct tcgccatccc gatcatctcg atgatcgagt tcaccttccg ggtgggtccg
     8701 gctggcggtt acaccttcga tcactggacc gccctcttcg acccggcgaa cgcgcgcaac
     8761 taccgcgacc tgttccagtc gatcggcaac tcgttcatcc tcgcggccgt cactgtcgcg
     8821 atcgtgctcg tcgtgctgct gcccacgatg atcctcgtgc acctgcagtt tccgcggctg
     8881 cggcgcgtgc tcgagttcat ctgcatcgtg cccatcacgg tgcccgcgat cgtgctcgtg
     8941 gtcggcctcg cgccggtcta ctcggtggtc gtgaaggtgt tcggcgggtc gatctggact
     9001 ctcgccttcg cgtacggcat caccgtgctt ccctacgcct accgctcgat ccaggcgaat
     9061 ctggatgccg taccggtcgt caccctcagc gaggccgccc gttcgctcgg tgcgggctgg
     9121 ctgtcggtgc tcggccgggt gctgctgccg aatctgcgcc gcggcgtgct cgccgccgcc
     9181 ttcatctcgg tcgcggtcgt gctcggcgag ttcacgatcg cctcgctgct caaccgccgc
     9241 aaccttcaga ccgctctgct gcaggtatcg cagtccgacc cgtgggtcgc cgtgatcctg
     9301 gcgttgctct cgctgctgtt cgcattcgtt ctgcttctac tgatcggcag gctgggctcg
     9361 ctcggccgag gcaaggagac caccccatga ccagcagcat cccnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnngcgagcg tgccgacgaa caagcgtgac atgggcatgg
     9541 tcttccagtc gtactcgctg ttcccgcaca tgaccgcgat cgagaacgtc gagttcgggc
     9601 tgcggatgcg caaggtcgcc accgccgagc gccgcactcg cgcgctcgag gcactcgaga
     9661 tggtcgggct cacgaccttc gccgagcgct tcgcacacca gctctccgga ggccagcagc
     9721 agcgggttgc gctcgcgcgc gccctcgtca cgcgccctcg ggtgcttctg ctggatgagc
     9781 ccctcagcgc cctcgacgcc aaggtgcggg tgcagctgcg cgacgagatc cggcgcatcc
     9841 agaccgagct cggaatcacc acggtgttcg tcacccacga ccaggaggag gcgctcgccg
     9901 tcgccgaccg ggtcgccgtc atgcgcgacg gggtgatcga gcagatcgga acgcccgagg
     9961 agttgtacac ggccccgcgc acccccttcg tcgccaactt cgtcggcctc agcaaccgtc
    10021 tggcggggga gcactcgaag ggcaccgtga cggtgcaggg cacgcggctc gacctgctcg
    10081 gcgatccgtt gcccgacgga ccggtcaccg cctacgtgcg ccccgaggac atcacgttcg
    10141 cggcacgcgg gatcgcggcg accgtggtgt cgtcgagctt cctcggatcg ttgcgtcgca
    10201 cccaggtgcg gctcgcggat gacaccctcc tctcgatcca gcacgacgtc gaaacccgtc
    10261 cgcaacccgg ggagctcgtg cacctcaaac tgcggggagt tcccgtcagc gccgtcgtcc
    10321 gcagctagtg cctcgcgcgg cgatctagac tgtccgcgtg gcacttggac gacggaagac
    10381 ggtcgcggca ccgcccgcgg atgaggcggt tccccccgcg ctgaaggtgg ctgcggcgta
    10441 ctcgtggcgc atcctcgtga tcgtcggtgt gatcgccgtt ctcgtcttcg tgatcatgca
    10501 gttccgcgac atcgtggtgc cgttcttcgt ggcggtgctg ctggccgcac tgctggttcc
    10561 gttcatgaac ttcctggtgc ggaagggctg gccccggtgg ctcgcgatcg tcgtcgcaat
    10621 ggtcggcact ctggccgtga tcgctgggct gatcgtgctc gtcgttcttc aggtgcgcgc
    10681 cggctacccc gatctgcagg cccagtcggt cgaggcctac gacaacctga aggcgtggct
    10741 gctcgcgtct ccgctccagc tcaccgaggc cgacatctcg gcctacctcg ggcaggcgtg
    10801 ggaggcgatc cagcgcgaca gctcggccct ggtctccggc ctcatgtcgg tgggaacgac
    10861 cgccggtcac gtgctggcag ggttcctgct cacgctcttc gcgacgctct tctttctcat
    10921 cgacggcaag ggagtctggc gctggatcgt gggcctcttc ccccgtcgct cacgccccgc
    10981 ggtggacgga gccggtcagg ccggatggat caccctcacg accttcgtga aggtgcagat
    11041 cttcgtggcc gccgtcgacg cgatcggcat cggcgccggt gcgtggattc tcgggctctt
    11101 cttcggggga ttcccgctgg tcatcccgat cgcggttgcg gtgttcctcg gctcgttcat
    11161 ccccgtggtc ggtgcgctca tctcgggagc gctcgccgtc ttcgtcgcgc tcgtgtacct
    11221 cggcccgata cccgccgtca tgatgctcgg aatcgtgctg ctcgtgcagc aggtcgaggg
    11281 gcacatcctg cagcccctgg tgatgggcac cgccgtcaag gtgcacccgc tcgccgtggt
    11341 cttcgctgtc gcagccggtg gcttcatcgc gggaatcccc ggggcgctct tcgccgtgcc
    11401 ggtgatcgcc gtgctcaacg tggtcgtcaa gtacatcgct gcgggggagt ggcgcaccca
    11461 cccgcatccg acagtaaagg atctcctggg cgatgcctga gaccgcagtg gttggcccca
    11521 ccctgggcgg cttcgaagag gcacgtgatc gtgtgaccgg tgtcgtgcag gtcaccctca
    11581 tggacagcag tcgctacctg agcgacatcc ttggttcgcc ggtgctactc aagtgcgaga
    11641 accttcagcg caccggctcc tacaagatcc gcggtgccta caatcggctc tccctgctca
    11701 ccgacgagga gaaggcgcgc ggcgtcgtcg ccgcctccgc aggcaaccac gcccagggcg
    11761 tcgcctacgc ggcccgcgag ctcggcatca aggcgaccat cttcatgccc gtcggcgttc
    11821 ccctgcccaa gctgcaggcc accaaggact atggggcgga ggtcgtgctc cgcggtcaca
    11881 ccgtcgatga gcccctgcgc gccgccgccg acttcgcccg caccacgggc gccgtgttca
    11941 tccacccctt cgaccacgag gacatcatcg ccggtcaggg aaccctgggg ctcgagatct
    12001 acgaacagca gcccgatgtc gacacggtcg tggttcccat cggcggtggt gggctcatct
    12061 cgggtgtcgc gagcgccatc aagcagcgcg ctgccatcga cggtcgcacc gtgcgggtca
    12121 tcggggtgca ggcggccaac gcgtcgccct atcccgtttc actcgagaag ggtgccccga
    12181 cccagatcac gatcctgccg acgatcgccg acggcatcgc cgtgtccaag cccggcgagc
    12241 tcaacttcga gatcgtgcgt cgggtcgtcg atgaggtcgt caccgtcagc gaggacgaca
    12301 tggcgcaggc gctgctcgtg ctgctggaac gcgcgaagct cgtagtcgag ccggccggtg
    12361 cggtgggagt ggcggcgatc ctcgccggac aggtcagcgc gagcggcacg accgtcgtca
    12421 tcctgagcgg cggcaacatc gatcccatga tcatggagag ggtgatcagc cacgggctgg
    12481 cggcctcgga gcgctacctc aagctgcgca tccctctgcc cgatcggccc ggccagctcg
    12541 cgcgcgtgtc ggcgctggtc gccgagtgca gcgcgaacgt cgtcgaggtg ctgcacaccc
    12601 gccacggtgg cggcctgctg atcagtcagg tcgaactcga actgcacatc gagacgcgcg
    12661 gtcccgagca cgccgagctc gtgctcgaac gccttcggga cgagggcttc gagccgcgca
    12721 tcgacttcta ggttggttga gtagcgccga aggcgcgtat cgaaaccac
//
LOCUS       DAOSOU010000037        12739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4922_length_12739_cov_5.884342, whole genome shotgun sequence.
ACCESSION   DAOSOU010000037 DAOSOU010000000
VERSION     DAOSOU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_12739_cov_5.884342"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLW15_03390"
     CDS             complement(<1..335)
                     /locus_tag="PLW15_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33477.1"
                     /translation="MAADFDPRFDPAFQRGYDGRTPGPVVEPVRPVVERVGSVVEPVE
                     TPPPPVELVETSAEPLRYETRGNPWLRALWVVAAVFTVAGMLAAYSAQAMMQGANTSS
                     AIDYYVLPLT"
     gene            395..2056
                     /locus_tag="PLW15_03395"
     CDS             395..2056
                     /locus_tag="PLW15_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT33478.1"
                     /translation="MTGQRIIIIGGVAGGMSAATRLRRLIEDADIVVLERGGHVSFAN
                     CGLPYYLGGVIGDRNDLLLQSPASLGARFALDVRVHTEVTGIDRAAKTVHVRNLITGI
                     DSVEPYDALVLSPGATPVRPPIPGGERMLTLRDIEDVDAAMAQLESAPQSALVIGAGF
                     IGLEMVENLVHRGLDVTLVELGDQVLPPLDIEMAGPVAERLREAGVDVRLGTQVTELG
                     SDSAVLSDGSVVGADFVIASIGVRPETSLAVGAGLELGERGGIRVDDQLRTSDPSIYA
                     IGDAVEKTDAISGEQRLVALAGLANRHGRLVADAISGKTITVRDALGTAVVGMMGLTI
                     AATGWNEKLLRSRGREIRVFHTHPASHAGYYPGAETMSLKLIADAATDEILGAQGVGG
                     DGVDKRIDIIATAMAGGITASELADLELAYAPQFSSAKDPVNMLGYVALNALDGLTGS
                     IQWHELEAAIDAGATLVDVRTPAEYADGAIPGSINVPLDDLRERFGELPAGRLIVHCK
                     VGQRGHTAVRLLAQLGRDAVNLDGGYLTWSAGTSARSDTRKPAFA"
     gene            2053..2655
                     /locus_tag="PLW15_03400"
     CDS             2053..2655
                     /locus_tag="PLW15_03400"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HOT33479.1"
                     /translation="MTNRTVIISVAAGVLVVGGALTAVALMNPPASTPVESLPSASAP
                     VAEPSTPTAPSTPTAEPYDTAASLLFIIEEEKLAHDVYVTLESTWGANIFANISDSET
                     THQGLLVPLLEARGIADPRSSEVGVFVDPDLQSLYNDLIARGATSLADAMQVGILIEE
                     KDIADLTVAMAAEDEADVISVYDRLLSGSENHLASFQRQA"
     gene            2754..3011
                     /locus_tag="PLW15_03405"
     CDS             2754..3011
                     /locus_tag="PLW15_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOT33480.1"
                     /translation="MDLNPDDMKPVLNRLKRAQGQLAAVIRMIDEGKDCTSVVTQLAA
                     VSSALDRAGFAIIASTLEQCVRQDDPELDKAKLEKLFLSLA"
     gene            3045..4517
                     /locus_tag="PLW15_03410"
     CDS             3045..4517
                     /locus_tag="PLW15_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HOT33481.1"
                     /translation="MADMSRRQVIVLGAIGAGVVVGGAGLLLGRPWDDRGEAPVDSAT
                     PSATAAGDAGTLREPVVMSSSSGRLALDLTAATGTVQIGGTGVHALTYNGTLPGPTLR
                     LKAGDRVALTFANQLDAPTNLHTHGLVVSPEGNSDNVFVMIDPGATHEYLYELGADHP
                     PGVFWYHPHHHGNSAEQLFGGLYGMIIVEDETPIDATRERVLVISDIGFDESGTLTVA
                     SNRDRMQGREGGTVMVNGQLDATLVARPGERERWRIVNACSSRYLRLRLDGQALTLLG
                     IDGGRFDDPRDIDELVLTPGNRADVLVTMAEGRSVLRTLPFDRGAMGMGAPLSNSTGA
                     DLATLTVAGVPSQTSALVLPPTAGRDLRLETATGTRTLTLAMGGGGGGGMSFSIDGKQ
                     FESSRIDTEVAAGTVEEWTINNTSTMDHPFHLHVWPMQLVEINGAAVSAVEYRDVVPV
                     PANGRAVVRIAFEGLTGTTVYHCHILDHEDQGMMGIIRAS"
     gene            complement(4781..5611)
                     /locus_tag="PLW15_03415"
     CDS             complement(4781..5611)
                     /locus_tag="PLW15_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT33482.1"
                     /translation="MGTIMVGVSESTSGHPAIGWAIRHARRRGDTVELVHVVDITWTT
                     IPVDWAESALLAAEEAVRGEAQRASLIDPSVAVHSVVLIGSPTRLLAEHAASADLLVV
                     GTHSRRAASEILLGSQATRIAAAATCPVAVIPAAGQEGTGIVVGVDGSEASAAAVAFA
                     AAEADRYGEPLTAIYAWSAPEPWGMGESAWPTSPVEEDRRILAEAVAGLADQYPDLRV
                     TAEVVGGRPLPALYAASVGARMLVVGTHGKHGFSKALLGSVSEDLVLSLPCPVVVVRT
                     "
     gene            5729..7069
                     /gene="radA"
                     /locus_tag="PLW15_03420"
     CDS             5729..7069
                     /gene="radA"
                     /locus_tag="PLW15_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOT33483.1"
                     /translation="MARPTTQFRCTECGWTSLKWVGQCGECQQWNTVVDATAPTQRVS
                     AVAPRSQARSITEIGAESVAHWPSGIAEFDRVLGGGVVPGAAILLSGEPGVGKSTLLL
                     EVASRAAASGQRVLYVSAEESVSQVRLRAERTHALQPSLFLAAETDLATILGQIDQVQ
                     PQLVIVDSVQTVSSGLTDGLAGGPSQVREVASTLIRVSKDRNLPVLLVGHVTKDGTIA
                     GPRLLEHLVDVVLQFEGDRQTALRFVRAHKNRFGPTDEVGCFEMTGDGIAEVADPSGL
                     FLSRARTPVSGTCVTIAVEGRRALPVEVQALIVKTQAPQPRRVVNGVDSSRVAMLLAV
                     LERRANLALSSFDVYVSTVGGIRLTEPGADLAIALAIASAYRDKAYPVTLAAVGEISL
                     AGEIRPASSAKQRSSEAKRLGFSQVLDSETVHLREALRLAFAGSTVERVDVPDF"
     gene            complement(7066..>8120)
                     /locus_tag="PLW15_03425"
     CDS             complement(7066..>8120)
                     /locus_tag="PLW15_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT33484.1"
                     /translation="LGLPQLRWWDAQAWTEHTSEARAPIIIQPATRLGYADDDLIAEE
                     SRPWDDLPSRREQRERERQDSGAIQLDYESDVLEYQPDTVDDELSAQPLLAMTLKELE
                     PPLTDTVDEETPGPRRASTHTNAAPAASTLSALAEEEEAPARAPKRMGTYTAAVWFIA
                     LMPAIQLAVSVLMIVGGLGNNYPLYLVVWFAPYLLVIGFAAFDRLVLTLWGHERPASA
                     WWAVLTEPVYLVMRAIRTYRQTGRGFAPLAAFVGSATAVLAGVLVLPGLVIAAIPGAF
                     AEEAARSVELDAAALGATFTVECPAPPLVIGDTFACIGTKADGTWDSISVRLTRQSGW
                     IDWRVEDWGPAIMVGN"
     assembly_gap    8121..8220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8221..8300)
                     /locus_tag="PLW15_03430"
     CDS             complement(<8221..8300)
                     /locus_tag="PLW15_03430"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT33485.1"
                     /translation="MPAGWYPDPLGLPQLRWWDAQAWTEHT"
     gene            8430..9176
                     /locus_tag="PLW15_03435"
     CDS             8430..9176
                     /locus_tag="PLW15_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33486.1"
                     /translation="MTIDTALTALAIVLSVVSVALSGWFSIRTARLNRQLEDERDERI
                     EAQSALKAAERVYEPLAQAAAELQSRIFNIVETGWVPLMKRYESHGDYALVSTAFLFA
                     HYFGWIEARRQAVLSSSGEGGRDISVQKLIDGVLKTLRRSEDSEGFLFFTTEQRAIGE
                     LMCEWEVVNKQRIPKVKGYATFVRMYRDDENFRAWFTPVNEGMSLVSKGDVRRLADIQ
                     RALVALIDELDPKRKYTAGYDLQPIHLEGL"
     gene            complement(9195..9572)
                     /locus_tag="PLW15_03440"
     CDS             complement(9195..9572)
                     /locus_tag="PLW15_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOT33487.1"
                     /translation="MPTFRSEPLESALAAQDVAAVAFALRNDRVAVPLIPGDGPPQVR
                     VFRRGEVDRYMLLLFSSLDSYQTMLPEESDLTVAAYDADALRDFLSQNIGVLESVWFD
                     VAGPHAMQAEPQDVLDALELSGE"
     gene            complement(9572..10177)
                     /locus_tag="PLW15_03445"
     CDS             complement(9572..10177)
                     /locus_tag="PLW15_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33488.1"
                     /translation="MSTFRNPVGPQPPSVYWRRRLVLGLGLLAVIIIIVLIVVRPGSG
                     APAPTPKPTGSNSASPAPTNAAGQVECDLTKVTLEATTDSNSYDPGVNPVLTFSLKSL
                     MTEPCTIAAGSDLQEFRITSGDELIWSSKDCQTGAVAANAVLKPGIPLQGPSVTWDRT
                     RSSTDTCDTTREEVIAGGASYHLEVVVGDLKSKNDTQFLLN"
     gene            complement(10267..10770)
                     /locus_tag="PLW15_03450"
     CDS             complement(10267..10770)
                     /locus_tag="PLW15_03450"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOT33489.1"
                     /translation="MSSFRVRPARTSDIPAIQGLVEPLVQRRILLGKDRVVLYEAVPE
                     FRVAEDENGALIGCGALHVMWEDLGEVRTLAVTEAWLGKGVGHALLEQVETDARGYGL
                     SRLFCLTFETDFFGRHGFEPIAEDVVAADVYTQLALSPDEGVAEFLDLSRVKQNTLGN
                     TRMLKRL"
     gene            complement(10875..>12739)
                     /locus_tag="PLW15_03455"
     CDS             complement(10875..>12739)
                     /locus_tag="PLW15_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT33490.1"
                     /translation="NNPVLIGEPGVGKTAVVEGLALAIVRGDVPETLKDKQLYTLDLG
                     SLIAGSRYRGDFEERLKKVTKEIRTRGDIITFIDEIHTLVGAGAAEGAIDAASILKPL
                     LARGELQTIGATTLDEYRKHFEKDAALERRFQPVQVHEPSLPHTINILKGLRDKYESF
                     HKVSITDGAIVAAANLADRYVQDRFLPDKAIDLIDEAGARLRLSILSAPPELREFDEK
                     IAEVRGQKEGAIEDQDFEKAASLRDEEKKLLGERLRLEKQWKSGDAGTTGTVDEGLIA
                     EVLAAATGIPVFKLTEEESSRLIFMEKALHMRVIGQEEAISVLAKTIRRTRAGLKDPK
                     RPSGSFIFAGPTGVGKTELAKALAEFLFDDENALISLDMSEYGEKHTVSRLFGAPPGF
                     VGFEEGGQLTEKVRRKPFSVVLFDEIEKAHPDIFNSLLQILEEGRLTDGQGRVVDFKN
                     TVIIMTTNLGTRDISGTPIGFTSSSDTATGYERMRGKVVEELKKHFKPEFLNRVDETI
                     VFPQLSKDELLQIVDLFIKRLGERLMDRDLTVELTVPAKEQLIKVGFDPSLGARPLRR
                     AVQHEIEDALSEKILHGELNAGDHVHVDFTDGEFIFTTGRQAGLEEKAPEAVEA"
BASE COUNT         2100 a         4250 c         4254 g         2035 t
ORIGIN      
        1 gtcaggggca gcacgtagta gtcgatcgcc gagctggtgt tcgcgccctg catcatggct
       61 tgggcggagt atgccgccag catgcctgcg acggtgaaca ccgcggcgac aacccacagt
      121 gcgcgcagcc acgggttgcc gcgagtttcg tagcggaggg gttcggcaga ggtctcgaca
      181 agctcgaccg gcgggggcgg ggtctcgaca ggctcgacca ccgaaccgac tcgctcgacc
      241 accgggcgga caggctcgac caccggcccg ggcgtgcgcc cgtcgtaccc ccgctggaac
      301 gcaggatcga accgggggtc gaagtcggct gccatgcctc agcttaaccc gtcgcctcct
      361 tgcggatacc cccgggggta tatagacttg cagcatgaca ggccagcgca tcatcatcat
      421 cggcggagtc gcgggcggaa tgtccgcagc cacccgcctt cgccgcctca tcgaagacgc
      481 cgacatcgtc gtgctcgaac gcggcggtca cgtgtcgttc gccaactgcg gccttcccta
      541 ctacctcggc ggagtcattg gcgaccgcaa tgatctccta ctgcaaagtc ccgcctcact
      601 cggcgcacgc ttcgccctcg acgttcgcgt gcacacggag gtgacgggca tcgatcgtgc
      661 cgcaaagacc gtgcacgtgc gcaacctcat cacaggcatc gacagtgtcg agccatacga
      721 cgccctggtg ctctcaccgg gggccacacc cgtacgtccg ccgatcccgg ggggcgagcg
      781 gatgctcact ctgcgcgaca tcgaagacgt ggatgccgcc atggcacagc tcgagtcagc
      841 gccgcagagc gcactggtga tcggtgcggg gttcatcggc ctcgagatgg tcgagaatct
      901 cgttcaccgc ggcctcgacg tcaccctcgt cgaactcggc gatcaggtac tccctccgct
      961 cgatatcgag atggctgggc cggtcgccga gcgactgcgc gaggccgggg tcgacgtcag
     1021 gctcggcacg caggtgaccg agctcgggtc tgacagcgcc gtgctgagcg acggcagcgt
     1081 tgtcggggct gacttcgtga tcgcctcgat cggcgtgagg cccgagacat ccctcgccgt
     1141 gggtgccggc cttgagctcg gcgagcgcgg cggcatccgg gtggatgacc agctgcgcac
     1201 cagcgacccc tcgatctacg cgatcggcga cgcagtcgag aagaccgatg ccatctccgg
     1261 tgagcagcgt ctggtcgcac tcgcgggtct cgccaaccgg cacggacgct tggtcgccga
     1321 cgccatctcc ggaaagacga tcaccgtgcg cgacgcgctc ggcaccgccg tcgtcggcat
     1381 gatgggtctg acgatcgccg ccaccggctg gaacgagaag ctgctgcggt cgcgaggccg
     1441 cgagatccgg gtgttccaca cccaccctgc ctcgcacgcc gggtactacc ctggcgccga
     1501 gacgatgtca ctcaagctca tcgctgacgc ggcgaccgac gagattctcg gcgcacaggg
     1561 cgtgggaggt gatggcgtcg acaagcgaat cgacatcatc gcgaccgcga tggccggtgg
     1621 catcaccgcc tccgagctcg ctgacctcga actggcctat gcgccgcagt tctcgtcggc
     1681 caaggacccg gtgaacatgc tcggttacgt cgctctcaac gcgctcgatg gtctgactgg
     1741 gtcgatccag tggcacgaac ttgaggctgc catcgacgcc ggcgcaaccc ttgttgatgt
     1801 gcgcacccct gccgagtacg ccgacggggc gatccccggt tcgatcaatg tgccgctcga
     1861 cgaccttcgt gagcgattcg gcgagctccc cgccggacgc ctgatcgtgc actgcaaggt
     1921 gggccaacgc ggccacacgg cggtgcgcct gctcgcccaa ctcgggcggg acgccgtcaa
     1981 cctcgacggc ggatatctca cctggagcgc gggcacgtcc gctcgctccg acactagaaa
     2041 gccggcgttc gcatgacgaa ccgtactgtc atcatctcgg tcgcagcagg agtactggtg
     2101 gtggggggcg cgctgactgc ggtcgctctg atgaaccctc ccgcatcgac tcccgtcgag
     2161 tcgctgccct cagcctcggc acccgtggcc gagcccagca cgccgaccgc cccgagcacg
     2221 ccgacggcgg agccatacga cacggccgcg tcgttgctgt tcatcatcga ggaggagaag
     2281 ctcgctcacg acgtctacgt caccctcgaa tcaacttggg gtgcaaacat cttcgcgaac
     2341 atctcggaca gcgagaccac ccatcagggg ctcctggtgc cgttgctcga ggctcgcgga
     2401 attgccgacc cgaggtcgtc ggaggtgggg gtcttcgtcg atcccgacct gcagtccctc
     2461 tacaacgacc tgatcgcgcg tggcgctacg agcctcgccg acgcgatgca ggtgggcatc
     2521 ctcattgagg agaaggacat cgccgacctc accgtcgcta tggcggccga agacgaggcg
     2581 gatgtcatca gcgtctatga ccggctgctc tcgggctccg agaaccacct cgcatcgttc
     2641 cagcgccagg cgtgacgcga ccgcacggcc gcgtcgtata gtgcggctct ataccccgca
     2701 gggtatgact cgataggggt atcatgaaag ctcgaatccg acctggagga acgatggatc
     2761 tcaaccccga cgacatgaag ccggtactca accgtctgaa gcgcgcgcag ggccaattgg
     2821 ccgccgtcat ccgcatgatc gacgagggca aggactgcac gagcgtcgtc acacaactcg
     2881 cggccgtctc gagcgcgctc gaccgggccg gcttcgcgat catcgccagc accctcgagc
     2941 agtgcgtgcg acaggatgac cccgagctgg acaaggccaa gctggagaag ctcttcctct
     3001 ccctcgcctg attacgctcg cgcgccgagt gcaaaggagc acgaatggct gacatgagtc
     3061 gccgccaggt catcgttctg ggcgcgatcg gcgccggtgt ggttgtcggc ggtgcgggcc
     3121 tgctgctcgg gagaccctgg gacgaccggg gagaggcccc ggtggacagc gccactccat
     3181 cggcaacggc agctggtgat gccggaacac tgcgggaacc ggtcgtgatg tcgagttcct
     3241 cggggcggct tgcgctcgac ctgacggccg ccacgggcac cgtgcagatc ggcggcaccg
     3301 gggttcacgc cctcacctac aacggcacgt tgcccggccc cacgctgcgg ctgaaggcag
     3361 gagatcgggt cgccctgacc ttcgccaatc agctggatgc tccgaccaac cttcacacgc
     3421 acggtctcgt cgtctctccc gaaggcaaca gcgacaacgt gttcgtcatg atcgacccgg
     3481 gcgcgactca tgagtacctc tacgaactcg gcgccgacca cccgcccggg gtgttctggt
     3541 atcaccccca ccaccacggc aactcggccg agcaactctt cggcgggctc tacgggatga
     3601 tcatcgtgga ggacgagacg ccgatagacg ccacacgcga gagagtgctc gtcatctcgg
     3661 acatcggctt cgacgagtcg ggcaccctga ccgtggcatc caaccgcgat cggatgcagg
     3721 gccgcgaggg cggcacggtc atggtcaacg gacagcttga tgccacgctc gtcgctcgac
     3781 ccggcgagcg cgagcggtgg cgaatcgtca atgcgtgctc gtcacgctac ctgcgcctgc
     3841 gactggacgg gcaagcgctg accctgctcg gcatcgacgg cggacggttc gatgaccccc
     3901 gcgacatcga cgagctcgtg ctcaccccgg gcaatcgcgc tgacgtgctc gtgaccatgg
     3961 ccgaggggcg gtcggtgctg cgcacactgc cgttcgaccg aggcgcgatg ggcatgggtg
     4021 caccactgag caacagcacc ggggcggatc ttgccaccct cacggtcgct ggcgtcccaa
     4081 gccagacgtc cgccctggtg ctcccgccga cagccgggcg cgacctccgt ctggaaactg
     4141 cgaccggaac ccgcacactc accctcgcga tgggcggtgg tggcggcggc ggaatgagct
     4201 tctccattga cggcaagcag ttcgagagtt cccgcatcga caccgaggtc gccgcgggca
     4261 ccgtcgagga gtggacgatc aacaacacca gcacgatgga ccacccattc caccttcacg
     4321 tgtggccgat gcagctcgtc gagatcaacg gggcggcggt gtcggcggtc gagtaccgag
     4381 atgtggtgcc cgtgcctgcg aacggtcgcg ccgtcgtccg tatcgccttt gagggcctca
     4441 ccggcacgac ggtttaccac tgccacatcc tcgaccacga ggaccaaggc atgatgggca
     4501 tcatccgagc ctcctagccc gacttggctt tgttataccc ccgggggtat actggaacca
     4561 ttcgatcagc catcaaggag gcatcatgtg ttcggcagta acgtgtcgca agtgcggtaa
     4621 gacgacctgg acgggctgcg gtcagcacgt cgaccaagtg atgaagggcg tgccgcagtc
     4681 gcagcgctgc cagggccacg agaacgagcc cagcaccggt ttcttctcgc gcctgttcgg
     4741 caagtagcgg ctgcagcccc agcacgcggc atccgcccgg tcaggtgcga accacgacca
     4801 ccgggcaggg cagcgacagc acgagatctt cactgacaga tccgagcagc gccttcgaga
     4861 agccgtgctt gccgtgggtg ccgaccacga gcatccgtgc ccccacactg gcggcgtaga
     4921 gagcgggcag cggtcggcct ccgaccacct cggccgtcac ccgcaggtcg gggtactggt
     4981 cggcgagacc cgcaacggct tcggcgagga tgcgacgatc ctcctccacc ggcgacgtcg
     5041 gccaggcgct ctcccccatg ccccacggct cgggggcgct ccacgcgtag atcgccgtga
     5101 ggggttcgcc gtacctgtca gcctcggcgg ccgcgaatgc gacggccgcg gccgaggcct
     5161 cggatccgtc gacgccgacg acgattcccg tgccctcctg gcccgcggcc gggatgaccg
     5221 cgacgggaca ggtcgccgcc gccgcaatgc gcgtggcctg actgcccaga agaatctcac
     5281 tggcggcccg tcgcgagtgc gttccgacga cgagcaggtc ggcgctggcc gcgtgctcgg
     5341 ccagcaggcg agtcggggag ccgatcagca cgaccgagtg cacggcaacg ctcgggtcga
     5401 tgaggctggc ccgctgtgct tcgccacgca cagcctcctc ggccgcgagc agagccgact
     5461 ccgcccagtc gaccgggatg gtcgtccacg tgatgtcgac gacgtgcacc agctccacgg
     5521 tgtcgccacg gcgccgcgca tggcggatcg cccacccgat cgcaggatgc cccgacgtgc
     5581 tttcgctgac tccgaccatg atcgtgccca tgagaatctc ctccgcagac ggcgctgtac
     5641 ttgccaccag cgtgcgccga gcgcgcgaca cgggttaggg ccgaaggtcc ttcgggacag
     5701 tgtcgcaacc ccaccgtagg cttgccgcat ggcacgcccc accacccagt tccgctgcac
     5761 cgagtgcggc tggacgagcc tcaagtgggt gggccagtgc ggcgagtgcc agcagtggaa
     5821 caccgtcgtg gatgcgacgg ctccgaccca gcgcgtcagc gccgtggccc cgcgcagcca
     5881 ggcccgctcg atcaccgaga tcggcgccga gtcggtcgcg cactggccga gcggcatcgc
     5941 cgagttcgac cgggtgctgg gcgggggcgt ggtcccgggg gcggcgatat tgctcagcgg
     6001 cgagccgggg gtcggcaagt cgacactgct gctcgaagtc gcttcgcgag ccgcggcatc
     6061 cggtcagcgg gttctctacg tcagcgccga ggagagcgtg agccaggtgc ggctgcgggc
     6121 cgagcgcact cacgcgctgc agccctcgct gtttctcgct gctgagaccg atctcgccac
     6181 gatcctcggc cagatcgacc aggtgcagcc ccagctcgtg atcgtcgaca gcgtgcagac
     6241 cgtgtcgtcc gggttgacgg atgggctggc cggtggccca tcgcaggtgc gcgaagtggc
     6301 atcgacgttg attcgcgtga gcaaggatcg caacctgccc gtgttgctcg tcggccacgt
     6361 caccaaagac ggcacgatcg cgggcccccg cctgctcgaa cacctcgtgg atgtcgtgct
     6421 gcagttcgag ggcgaccgtc agacggcgct gcggttcgtg cgcgcccaca agaaccggtt
     6481 cggaccgacc gacgaggtcg gctgcttcga gatgaccgga gacggcatcg ccgaggtcgc
     6541 cgacccgagc gggctgttcc tctcgcgtgc gcgcacgccg gtgagcggaa cctgcgtgac
     6601 gatcgccgtc gagggccgac gggcgctgcc ggtcgaggtg caggcgctta tcgtgaagac
     6661 gcaggcgccc cagccgcggc gcgtggtcaa cggcgtcgac tcgtcgcggg tcgccatgct
     6721 gctcgcggtg ctcgagcggc gcgcaaacct cgcgctgtcg agcttcgacg tgtacgtgtc
     6781 caccgtcggc ggcatccgcc tcaccgagcc gggcgccgat ctcgccatcg ccctcgcgat
     6841 cgcgagcgcc taccgcgaca aggcctaccc cgtgacgctt gcggcggtcg gtgagatcag
     6901 cctcgccggt gagatccggc cggcgtcatc cgccaagcag cggtcgtcgg aggcgaagcg
     6961 actgggcttc agccaggtgc tcgacagcga gactgtgcac ctgcgggagg cgttgcggct
     7021 cgcgttcgcg ggctcgacgg tggaacgggt cgacgtaccc gacttctagt tgcccaccat
     7081 gatcgccggg ccccagtcct cgacgcgcca gtcgatccag ccggactggc gggtgaggcg
     7141 caccgagatc gaatcccagg tgccgtccgc cttggtgccg atgcaggcga aggtgtcacc
     7201 gatgaccaga ggcggggcgg ggcactcgac ggtgaacgtg gcaccgagag ccgcagcatc
     7261 caactcaaca gagcgggccg cctcttcggc gaaggcgccg gggatggcgg cgatcacgag
     7321 accgggcagc acgagcacgc cagccagtac tgcggtggca gagccgacga atgcggccag
     7381 cggcgcgaac cccctgccgg tctgacggta ggtgcggatc gcacgcatga ccagatacac
     7441 cggctcggtg agcacggccc accacgcgct ggccggccgt tcgtgacccc agagggtcag
     7501 caccagccgg tcgaatgcgg cgaaaccgat cacgagcaga tacggcgcga accacaccac
     7561 caagtagagc gggtagttgt tgccgaggcc gccgacgatc atgagcacgc tcaccgcgag
     7621 ctggatcgcg ggcatcaggg cgatgaacca gacggccgcc gtgtaggtgc ccatgcgctt
     7681 gggtgcgcgc gcgggcgctt cctcttcctc ggcgagtgcg ctcagggtgg atgccgcggg
     7741 cgcagcattg gtgtgagtgc tcgcgcggcg cggtccgggc gtctcctcat cgacggtgtc
     7801 ggtgagcggc ggctcgagtt ccttgagcgt catcgcgagc agcggctggg cgctgagttc
     7861 gtcgtcgacc gtgtcgggct ggtactccag aacatcggac tcgtagtcga gctggatggc
     7921 tcccgaatcc tggcgctcgc gttcacgctg ctcacggcgc gacggaaggt cgtcccaggg
     7981 gcgggactcc tcggcgatca gatcgtcgtc ggcgtagccc agacgagttg cgggctggat
     8041 gatgatgggt gcacgcgcct cggaagtgtg ctcggtccac gcctgagcgt cccaccagcg
     8101 cagctgggga agaccgaggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 gtgtgctcgg tccacgcctg agcgtcccac cagcgcagct ggggaagacc gagggggtcg
     8281 ggataccacc cggccggcac cccgaagttg ttgtcatcca ctcacatagc gtaaccggca
     8341 aacctattcg acagaaagta ttgtctaccc cgggtgacac ccccagttct ggtaaccccc
     8401 agcagaaccc ccaccgctac catcgacgca tgacgatcga cacagcactg accgcgctcg
     8461 cgatcgtgct gagcgtggtg agcgtcgccc tctcgggatg gttctcgata cgcaccgcgc
     8521 gtctcaaccg tcagctcgaa gacgagcgcg atgagcgtat cgaggcacag tccgccctca
     8581 aagccgccga gcgcgtgtat gagccgctcg cccaggctgc ggccgagctg cagtcccgca
     8641 tcttcaacat cgtggagacc ggatgggtgc cgctcatgaa acggtacgag agccacggcg
     8701 actacgccct ggtgagcact gcgttcctgt tcgcccacta cttcggctgg atcgaggccc
     8761 gccgtcaggc cgtgttgtcg tcgagcggcg agggcggtcg cgacatctcg gtgcagaagc
     8821 tcatcgacgg tgtgctgaag actctgcgcc gcagcgagga cagcgagggc ttcctcttct
     8881 tcaccaccga gcagcgcgcg atcggcgaac tcatgtgcga gtgggaagtg gtcaacaagc
     8941 agcgcatccc caaggtcaag ggctacgcga cattcgtgcg gatgtaccgc gacgacgaga
     9001 acttccgcgc ctggttcaca ccggtcaacg aggggatgag tctggtgtcg aagggcgacg
     9061 tgcgtaggct cgccgacatc cagcgcgccc tcgtggcact catcgacgag ctagacccca
     9121 agcgcaagta cacggccggc tacgaccttc agcccatcca cctcgagggg ctgtaggcct
     9181 cccgcgccgg ggcgctactc gccgctgagc tcgagcgcgt cgagcacgtc ctgcggctcg
     9241 gcctgcatgg cgtgcggacc ggccacgtcg aaccagaccg actcgagcac gccgatgttc
     9301 tgactgagga agtcccgcag ggcgtccgcg tcgtaggcgg cgacggtcag atccgactcc
     9361 tccggaagca tggtctggta gctgtcgagc gatgagaaca gcagcagcat gtagcggtcc
     9421 acctcgccgc ggcggaagac gcgcacctgg ggcgggccgt cacccgggat caacggcacc
     9481 gccacgcggt cgttgcggag agcgaatgct acggccgcga catcctgagc ggcgagcgca
     9541 ctctcgagcg gctcggaacg gaaagtcggc atcagttgag caggaactgg gtgtcgttct
     9601 tggacttcag gtcaccgacg acaacctcga ggtggtagct cgcacccccg gcgatgacct
     9661 cctcgcgggt ggtgtcgcag gtgtccgtcg acgaacgggt gcgatcccag gtgaccgatg
     9721 gtccctgcag cgggatgccc ggcttcagca cggcgttggc cgcgaccgct ccggtctggc
     9781 agtccttcga gctccagatc agctcatcgc cgctggtgat gcggaattcc tgcagatcgg
     9841 atccggcggc gatcgtgcac ggctcggtca tgaggctctt gaggctgaag gtcagcaccg
     9901 ggttcacgcc cgggtcgtag ctgttcgagt cggtagtcgc ctcgagcgtg accttggtga
     9961 gatcgcactc gacctgacct gcggcgttgg tcggcgcggg gctcgccgag ttggacccgg
    10021 tgggcttcgg cgttggggcg ggtgcacccg aacctggcct caccacgatc agcacgatga
    10081 tgatgatgac ggcgagcaag ccgagcccca gcaccaggcg gcgacgccag tagacgctgg
    10141 gcggctgcgg gccgaccgga ttgcggaacg tcgacatgcc ctcagggtaa gcgccgccgc
    10201 tggggatgac ccggcgattc cccggcgtgc ccctaggccg ggcggcccaa ccgtgccgct
    10261 cggtgcctag aggcgcttga gcatgcgcgt gttgccgagg gtgttctgct tcacccgcga
    10321 cagatcgagg aactcggcca cgccctcgtc gggggagagt gcgagctgcg tgtagacgtc
    10381 ggccgcgacc acgtcctccg cgatcggctc gaatccgtgt cgcccgaaga agtcggtctc
    10441 gaaggtgagg cagaacagcc gcgagaggcc gtagccccgg gcatccgtct cgacctgctc
    10501 gagcagggcg tgcccaacac ccttacccag ccacgcctcg gtgacggcga gggtgcgcac
    10561 ctcgccgagg tcctcccaca tcacgtggag ggcgccgcag ccgatgaggg cgccgttctc
    10621 gtcctcggcg acgcggaact cgggcaccgc ctcgtagagc acgacgcgat ctttgccgag
    10681 caggatgcgc cgctgcacga gcggttcgac caggccctgg atcgcgggga tgtcgctggt
    10741 gcgggcgggg cgcacgcgga aggaactcac ggagaaaaga ctaaggccgg ttgagtagcg
    10801 cggagcgcgt atcgaaacct cgccatcggg tttcgatacg ccgactccgt cggctactca
    10861 accggccttg tggattacgc ctcgacggcc tccggcgcct tctcctcgag tccggcctga
    10921 cgaccggtcg tgaagatgaa ctcgccatcg gtgaagtcga cgtgcacgtg gtcgcccgcg
    10981 ttcagctcgc cgtggaggat cttctcgctc agggcatcct cgatctcgtg ctgcactgcg
    11041 cggcgcaacg gccgggcacc cagcgacggg tcgaagccca ccttgatgag ctgctccttg
    11101 gccggaaccg tcagctcgac cgtgaggtcg cggtccatca ggcgctcacc gagccgcttg
    11161 atgaagaggt ccacgatctg cagcagctcg tccttgctca gctgcgggaa gacgatggtc
    11221 tcgtcgacgc ggttgaggaa ctcgggcttg aagtgcttct tcagctcctc gacgaccttg
    11281 ccgcgcatcc gctcgtaacc ggtggcggtg tcgctcgacg acgtgaagcc gatgggcgtg
    11341 cccgagatgt cccgggtacc gaggttggtg gtcatgatga tcacggtgtt cttgaagtcg
    11401 acgacgcgac cctgaccatc ggtcaaccgg ccctcctcca gaatctggag cagcgagttg
    11461 aagatgtcgg ggtgggcctt ctcgatctcg tcgaacagca ccacgctgaa cggcttgcgg
    11521 cgcaccttct cggtgagctg gccgccctcc tcgaagccga cgaacccggg aggggcaccg
    11581 aacagccgcg agacggtgtg cttctcgccg tactcgctca tgtcgagcga gatgagcgcg
    11641 ttctcgtcat cgaagaggaa ctcggcgagc gccttggcga gctcggtctt tccgacgccg
    11701 gtggggccgg cgaagatgaa cgagcccgag gggcgcttcg ggtccttgag tccggcgcgg
    11761 gtgcggcgga tcgtcttggc gagaaccgag atggcctcct cctggccgat gacgcgcatg
    11821 tgcagcgcct tctccatgaa gatgagcctc gacgactcct cctcggtcag cttgaagacc
    11881 gggatgcccg tggccgcagc cagcacttcg gcgatgagtc cctcatccac cgtgcccgtg
    11941 gttccggcgt cgccggactt ccactgcttc tcgaggcgca ggcgctcgcc gaggagcttc
    12001 ttctcctcgt cgcgcaggct cgcggccttc tcgaagtcct ggtcctcgat ggcgccttcc
    12061 ttctggccac gcacctcggc gatcttctcg tcgaactcgc gcagctccgg cggggccgac
    12121 aggatcgaca ggcgcaggcg tgcgccggcc tcgtcgatca ggtcgatggc cttgtcgggc
    12181 aggaagcggt cctgcacgta gcggtcggcg aggttcgcgg cggcgacgat cgcgccatcc
    12241 gtgatcgaca ccttgtggaa cgactcgtac ttgtcgcgca gtcccttgag gatgttgatg
    12301 gtgtgcggca gcgacggctc gtgcacctgc acgggctgga agcggcgctc gagcgcggca
    12361 tccttctcga agtgcttgcg gtactcgtcg agggtggtcg caccgatcgt ctgcagctca
    12421 ccgcgggcga gcaggggctt caggatcgac gcggcgtcga tcgcgccctc ggcggcaccg
    12481 gcacccacga gggtgtggat ctcgtcgatg aacgtgatga tgtcaccgcg cgtgcggatc
    12541 tccttggtga ccttcttgag gcgctcctcg aagtcaccgc ggtagcggct gccggcgatg
    12601 agcgaaccca ggtcgagcgt gtagagctgc ttgtccttta gcgtctcagg aacgtcgccg
    12661 cgaacgatcg ccagcgcgag cccctcgacg acggcggtct tgccgacgcc gggctcaccg
    12721 atcagcacgg ggttgttct
//
LOCUS       DAOSOU010000038        12715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_4933_length_12715_cov_6.007899, whole genome shotgun sequence.
ACCESSION   DAOSOU010000038 DAOSOU010000000
VERSION     DAOSOU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12715
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4933_length_12715_cov_6.007899"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1466
                     /locus_tag="PLW15_03460"
     CDS             252..1466
                     /locus_tag="PLW15_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007541284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT33491.1"
                     /translation="MTRQLPQDPIVRELVVQARRMQFSRRNILQGAGIGAGALALAAC
                     APTSAGAPKPAEDISATDKTLTWSNWPAYIDEDDDGNYPTLVRFQDQTGISVEYKVDI
                     DDNNTYYGTIKDQLALGQFTGADTFCLTEWMVARLIRFGYVQDLDAANIPNKKNLAPA
                     LLNPDFDPGRAKSLPWQNGFAGLAWNTEEIPAGIKSVDDLWKPALKGKVTVLSEMRDT
                     MGILLLSQGVDISSEDWGTDEYQAAIAVLEENVSSGQIFGIKGNEYMQDFVSGDAIAA
                     IAWSGDITVLNAEAGYEKWKFALPETGGTLWGDTFVVPMGSPRKANAETLMNYYYEPE
                     VAAEVAAWVNYITPVVGAQEVAAEKFPEVADNQLIFPDATTLSNAHIFRTLDSADDQA
                     FNAEFQRVKLGA"
     gene            1466..2665
                     /locus_tag="PLW15_03465"
     CDS             1466..2665
                     /locus_tag="PLW15_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33492.1"
                     /translation="MAAGAFAEAGADLELVGLHKRFPGFTAIEDLDLTIPAGSFFALL
                     GPSGCGKTTTLRLVAGLEQPTSGRILIGGKDVTSVKSFKRPVNTVFQSYALFPHMTVL
                     ENVSFGLKRRGIADATGKAHEALRLVELDHLSARKPQQLSGGQQQRVALARAVVNRPA
                     LLLLDEPLGALDLKLRRQMQLELKAIQNEVGLTFLHVTHDQEEAMTMADTVAVMNKGQ
                     IEQMGAPEELYELPKTAFVANFLGQSNLFTGDVTNSKGDSIVVDIAGVKIEVPKDRAQ
                     RHTGEITIGVRPEKLMLHTEAPKKAPGVNILGPGRVTDVSFSGVSTQYQIAVPGIGLI
                     TVFAQNMVFGPVVNLGAEAWVSWTVDHGFGLLDEPEHTPKFSADTDTATIALQTRHEL
                     LAEIEEA"
     gene            2669..3583
                     /locus_tag="PLW15_03470"
     CDS             2669..3583
                     /locus_tag="PLW15_03470"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33493.1"
                     /translation="MAFAAFGSTAGPVEQAPRRRSWVALVLLLPGLAYLALFFLTPLV
                     SLVLTSFQEPVEFGDVGQFQQAFKIENYATVIEAYWPHFLRAFGYAIVATVAALLIGY
                     PLAYFIGVKCRSKPLLQAMLLTLVIAPFFISFLLRTLAWKQLMADDTPLVQALKSLSV
                     LPADGHLTGTAFSVIFGLTYNFLPFMTLPIYTTLERLDTRYLEAGADLYASPATTFWK
                     VTLPLSLPGIVSGTLLTFIPASGDYINASSSFLGSAQTQMVGNAIEANFLGLLNYPEA
                     AALSVILMGLILVIVGIYVRRTGTEDLL"
     gene            3580..4380
                     /locus_tag="PLW15_03475"
     CDS             3580..4380
                     /locus_tag="PLW15_03475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33494.1"
                     /translation="MRFSLGKWLLPLYSALALIFLLTPIVYTFVFSFNDSVKSNIVWV
                     GFTLDNWTNVCDAQGVCAAFVTSILVGVVATLIATVLGTLLAIALARYRFRGRSVISL
                     LLFLPMATPEVVLGAGLAAQFLTAGVPKGLGTIIIAHALFCLSFVVVTVKARVASLDP
                     ALEEAGRDLYGSAFQVFTRITLPLLMPGIIAAALLSFALSFDDFIITRFNNGGVETFP
                     VFVYTAAARGMPPEANVIASAVFILALVIVVVAQTTSAARRKRLARAQ"
     gene            4465..5064
                     /locus_tag="PLW15_03480"
     CDS             4465..5064
                     /locus_tag="PLW15_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT33495.1"
                     /translation="MPDPADIAAPIPPPRPETFVSVDIETSGPTPGRYSMLSVGACLV
                     DVPEHGFYVELKPTSRLVVESAQAVGGLSLDDLARDGVDPAEAMARFADWLEAEVPAG
                     TVPVFVGFNAAFDWMFVADYFDRFLGRNPFGHAALDIKSYTMGMAGITWAQTSMRFLA
                     PKYLNGRKLTHNALGDARDQAEIFAAVRAESARSQPPGV"
     gene            5093..6421
                     /locus_tag="PLW15_03485"
     CDS             5093..6421
                     /locus_tag="PLW15_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33496.1"
                     /translation="MSDTSDADVRNIVESARRLGIELDEVGTIQWLTAMAASQDDDQI
                     VVDTASGTFGHKVSMLDFSPRDLERFRRIGQIVEVTGPEGVAESALALSGSAAQSKIQ
                     SFPGDCDYFQRLNIKADTREQACGIMAGLMRDKVLSTLQGPTYQFLEAKLGSFAWDCT
                     QNGEKRWKGSPITWKAADIAAGTIDVVKDDGAAVTLRWDEVALDPGWCKLDWVVADPA
                     RTQLSNASNVIDVTWEAPSGEIVSLDGYLDAFFQEVYLDSDDVPVFAKLAKFVSDDAL
                     DEYVDRLEGEVRKYLTDHLNYGKAAKRMYNVFRLSGRHLDAAFVRELFDEPTTILYQV
                     WSLIVTLENATEPGSSIPIEAVQAQADTLIVDVVKALDGDQEDAIVQALLQLRRTLED
                     QNSGEARNSEVEAAQAQVVNLINTFYRDKLVAMPSIKEYIDAVQAEGPAH"
     gene            complement(6431..7408)
                     /locus_tag="PLW15_03490"
     CDS             complement(6431..7408)
                     /locus_tag="PLW15_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOT33497.1"
                     /translation="MSWSAAGDPELDNLSAAGVVPLAAVHRSRMRESIHLGAAAVVGP
                     DGEVVRALGDIDALIYPRSTLKFLQAITVLRSGVDLDGPELVLAAASHTGTAAHVEVV
                     RSILHRAGLDESALQCPADWPSDPNSRDEASGPARITMNCSGKHAAFLLACVRNGWPI
                     DSYLAARHPLQLIVQRTVEDYTGEPVDHTGIDGCGAPLHAVSLRGLARATGLVARGAD
                     ADAERLSRAIRRDPWALDSPAVASVMSELGLIAKNGAEGVFVAGSPDGTAVALKIIDG
                     STRASIPVALALLDEQGVVDSERATSVLAATTQPVLGGGEPVGELIALV"
     gene            complement(7405..8748)
                     /gene="gabT"
                     /locus_tag="PLW15_03495"
     CDS             complement(7405..8748)
                     /gene="gabT"
                     /locus_tag="PLW15_03495"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HOT33498.1"
                     /translation="MTDTLAPTTSAVVQERRVVTAIPGPKSQALHERRLKVVPVGVTA
                     ALPVYIERAHGAILVDVDGNQFIDLGAGIGVTTIGHTDDAVVAGATEQLEKLTHTLFT
                     ITPYEGYVRVAEYLAAHTPGDFAKKTVLVNSGAEAVENGVKIARKYTGRNGVAVLEHA
                     YHGRTNLTMAMNFKASPYSLGFGPFAGDVYRAPNSYPYHDGLSGADAAARTISYLEKT
                     VGAADLACLVVEPIQGEGGFQVPADGYLPALQAWCTANGIVFIADEIQSGMARTGKYF
                     ASEHFGLVPDMVLSAKGIAGGLPLAGVTGRAEIMDSAQPGGLGGTFGGNPVSAAAAIA
                     VFEQIEQKNLLAEAVRIENALKGALLELQKKYPVIGDVRGIGAMIAIELVQPGTHEPN
                     AAAVSAISAFAAQKGVLLLTAGTYGNVLRFLPSLAISNELIAEAVGVIDEAFASL"
     gene            8789..9262
                     /locus_tag="PLW15_03500"
     CDS             8789..9262
                     /locus_tag="PLW15_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT33499.1"
                     /translation="MQIEHRFEVQLEWQGNRGTGTSNYRSYGRDHVISSPGNPPIQGS
                     SARVFHGDADRWNPEEMLIASLSQCHMLSYLHVATANGIVVESYRDDATGTIEQTADG
                     GGHFTGATLRPVVEISAGEPELALALHREANEKCFIASSMNFVVAHEPIIHVVAP"
     gene            9347..10324
                     /locus_tag="PLW15_03505"
     CDS             9347..10324
                     /locus_tag="PLW15_03505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT33500.1"
                     /translation="MNVTPRRAALSLLVTAGLVASLAGCTTGSTGSSDCAATPSGSVS
                     NAVKVSGDFGTKPDVTINFPTAAPATERTVVINGDGDTVVAGDNVNVDYALFDGETGV
                     ELAATEYTEGSTVEFPVDDTQFLVGIVKTLECSTVGSRVVGVIPAADSFGEAGSDQLG
                     VAPGDDIVFVVDVVSISAPKIPALPKADGADQPATPGFPTVVLDDTGRPTVTIPDEAP
                     PTELKLAVLKKGTGAVVADGDDVTVHYVGVNWNTKVIFDESWARGEPATFNTGQVIAG
                     FTAALVGQKVGSQVIVIIPPDQGYGSAGSPPDIGGTDTLVFVIDILGIG"
     gene            10343..11419
                     /locus_tag="PLW15_03510"
     CDS             10343..11419
                     /locus_tag="PLW15_03510"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT33501.1"
                     /translation="MRRVIILGSTGSIGTQALEVIAANPHLFEVVGLAAGSNREAVGA
                     QAAEFSVEHIAFGADEASQLVRTVQADVVLNGITGSVGLGPTLAVLETEAVLALANKE
                     SLIVGGELVTSRVRPGQLVPVDSEHSAIAQALRSGTADEVSRLVLTASGGPFRGRSRE
                     SLREVTPREALAHPTWDMGLVVTTNSATLVNKGLEVIEAHLLFGVPYDRIEVTVHPQS
                     IVHSMVEFVDGSTIAQASPPDMRLPISLGLDWPNRVAGVGRPLDWTVSSQWTFEPLDT
                     EVFPAVALAKRVGEAGRTYPAVFNAANEQAVLAFHAGRIGYLDIVDTVERVVDAHSAD
                     EFSLESVFEAERWARAEADRLLTV"
     gene            11438..>12715
                     /locus_tag="PLW15_03515"
     CDS             11438..>12715
                     /locus_tag="PLW15_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT33502.1"
                     /translation="METVLLYILGILIIVVGLALSIGLHELGHLIPAKRFGVRVGQYM
                     IGFGPTIFSRMKGETEYGLKAIPLGGYISMSGMYAPAHEGGRGRTATTSMFETLVQDD
                     TKADDDEGRTFFQLPVFKRVIIMLGGPVMNLLIAVVLYTVILCGFGIAQTTTTIGSVS
                     QCVVAASSQSQECTSADPVAPGVEAGIKPGDRIVSMNGQAIGTWDDATAIIRASAGKS
                     IDIVVDRAGAEVALTATPLLTERYVYDDKGAPVKGADGKQVTEEVGFLGIGSATAIVQ
                     QPVTAVLPTVGDRVVGIISVITQLPQRLVDVAVAAFGPGDRDPNGLIGVVGVGRLAGE
                     ITSMSDIPIASRAAGIIEVVASLNIALFVFNLIPLVPLDGGHIAIALIDGIRRFFARI
                     FKRPEPGPIDATRILPVTLVVTVLLGAMTLLLVY"
BASE COUNT         2111 a         4263 c         4091 g         2250 t
ORIGIN      
        1 gaccaggtcg tgcacgcctg ccggcgtgat cgcaacacgg aactcgttgt tcttaatctc
       61 tcggggaacg gcgaccttca tgcccaacag cgtactgggc gtacaccatg gattccgcac
      121 aaatttcggg cgtcttcatg ctgatttcgt gaaatttgtg ttacgaagtg cgccttttgc
      181 tccctcgggg tgtgtctggg gactgacctg tgtcagtatc gacgcacgcg tgaaccgaag
      241 gagctgcccc gatgactaga caacttcccc aggacccgat cgtccgtgag ctcgtcgtgc
      301 aggcgcggag gatgcagttc tcccggcgca acattctgca aggcgcgggt atcggcgccg
      361 gtgctcttgc ccttgccgcc tgtgctccga ccagcgccgg agcgccgaag ccggccgagg
      421 acatctcagc caccgacaag actctgacgt ggtcgaactg gcccgcctac atcgacgagg
      481 atgacgacgg caactacccg accctcgtgc gcttccagga tcagacgggc atcagcgtcg
      541 agtacaaggt cgatatcgac gacaacaaca cctactacgg cacgatcaag gatcagctgg
      601 ctctcggtca gttcaccggg gcggacacct tctgcctgac cgagtggatg gtcgcccgcc
      661 tcatccgctt cggttacgtg caggatctgg atgccgcgaa catcccgaac aagaagaacc
      721 tcgcccccgc cctgctcaac cccgacttcg accccggtcg cgccaagtcg ctgccctggc
      781 agaacggttt cgcgggcctc gcgtggaaca ccgaggagat cccagccggc atcaagagcg
      841 tcgacgacct gtggaagccc gcgctcaagg gcaaggtgac ggtgctcagc gagatgcgcg
      901 acacgatggg catcctcctg ctgagccaag gcgtcgacat ctcgtcggag gactggggca
      961 ccgacgagta ccaggccgcg atcgctgtgc tcgaggagaa cgtctcatcc ggacagatct
     1021 tcggcatcaa gggcaacgag tacatgcagg acttcgtctc gggagatgcc atcgccgcga
     1081 tcgcctggtc tggcgacatc acggtgctca atgccgaggc cgggtacgag aagtggaagt
     1141 tcgcactgcc cgagaccggc ggaactctgt ggggtgacac cttcgtcgtg cccatgggct
     1201 ccccgcgcaa ggccaacgcc gagacgctca tgaactacta ctacgaaccc gaggttgccg
     1261 ccgaggtcgc agcatgggtg aactacatca cccccgtcgt cggtgcacag gaggtcgccg
     1321 ccgagaagtt ccccgaggtt gccgacaacc agctgatctt cccggatgcc acgaccctgt
     1381 ccaacgcgca catcttccgc acgctcgact cggctgacga tcaggcgttc aacgcggagt
     1441 tccagcgcgt caagctgggc gcctgatggc cgctggtgca ttcgcggagg cgggcgcgga
     1501 tctcgagctg gttggtctgc acaagcggtt ccccgggttc acggcgatcg aggatctcga
     1561 tctcaccatc cccgcgggct cgttcttcgc gctgctcggc ccgtccggtt gcggcaagac
     1621 gaccacgctg cgactggttg ccgggctcga gcagccgacg tcgggtcgca tcctgatcgg
     1681 tggcaaggat gtcacgtcgg tcaagagctt caagcggccg gtcaacacgg tgttccagag
     1741 ctacgcgctg tttccgcaca tgaccgtgct cgagaatgtg tcgttcggcc tcaagcgtcg
     1801 tggaatcgcg gatgccacgg gcaaggccca cgaagcactg cgcctggtcg aactcgacca
     1861 cctctcggcg cgcaagcctc agcagctgtc cggtgggcag cagcagcgcg tcgcgctcgc
     1921 ccgagcggtc gtcaatcgcc cggcactgct cctgctcgat gagccgttgg gcgcactcga
     1981 cctcaagttg cgccggcaga tgcagctcga actcaaggcc attcagaacg aggtggggct
     2041 gaccttcctc cacgtcaccc acgatcagga agaggccatg accatggccg acacggtcgc
     2101 ggtcatgaac aagggccaga tcgagcagat gggtgccccc gaggagctgt acgagttgcc
     2161 caagacggca ttcgtcgcca acttcctggg ccagtccaat ctgttcacgg gagacgtcac
     2221 caactcgaag ggcgactcga tcgtcgtcga cattgccggc gtcaagatcg aggtgccgaa
     2281 ggatcgggcg cagcgtcaca ccggcgagat caccatcggc gtgcgtcccg agaagctcat
     2341 gctgcacacc gaagcgccga agaaggcgcc gggcgtcaac atcctcggcc ctggtcgggt
     2401 gacggatgtc tcgttctcgg gcgtcagcac ccagtaccag atcgccgtac cgggcatcgg
     2461 actcatcacc gtcttcgccc agaacatggt cttcggtccc gtcgtgaatc ttggcgcgga
     2521 ggcctgggtc agctggacgg tcgatcacgg gttcggtcta ctcgatgagc cggagcacac
     2581 tccgaagttc tcggccgaca ccgacaccgc cacgatcgcg ttgcagaccc ggcacgagct
     2641 tctcgcggag atcgaggagg cctagcgcat ggccttcgct gcattcggct cgacggccgg
     2701 tccggtcgag caggcgccac gtcggcgcag ttgggtggcg cttgtcctgc tgctgccggg
     2761 gctcgcctac ctcgccctgt tctttctcac gcccctcgtc tcgctcgttc tgacctcgtt
     2821 ccaggagccg gtcgagttcg gcgatgtcgg ccagttccaa caggcgttca agatcgagaa
     2881 ctacgccaca gtgatcgagg cctactggcc gcacttcctg cgcgcgttcg gctacgcgat
     2941 cgtggccacc gtcgcggcgc tgctcatcgg ctacccgctc gcctacttca tcggcgtgaa
     3001 gtgccggtcg aagcccctcc tgcaggcgat gctgctgacg ctcgtcatcg ccccgttctt
     3061 catctccttc ctgctgcgca ccctcgcctg gaagcagttg atggcggatg acacccctct
     3121 cgtgcaggca ttgaagtcgc tctccgtgct gcccgccgac gggcacctga ctggcacggc
     3181 cttctcggtc atcttcggcc tgacctacaa cttcctgccg ttcatgaccc tgccgatcta
     3241 cacgacgctc gagcgcctcg acacccgcta cctggaggcg ggagccgacc tgtacgcgag
     3301 ccctgcgacg acattctgga aggtcaccct gccgctgtca ctgccaggga tcgtctcggg
     3361 cacgctgctg accttcatcc cggcgtccgg tgactacatc aatgcgagtt cgagcttcct
     3421 cggatccgcg cagacccaga tggtcggtaa cgcgatcgag gccaacttcc tcggcttgct
     3481 caactacccg gaggccgcag ctctgtcggt catcctgatg ggtctgattc tcgtgatcgt
     3541 cggcatctac gtgcgacgca ccggaacgga ggacctgctg tgagattctc actcggcaag
     3601 tggctgctgc cgctctattc ggcgctagcg ctcatcttcc ttctcacgcc gatcgtctac
     3661 accttcgtct tctcgttcaa cgattcggtg aagtccaaca tcgtctgggt gggcttcaca
     3721 ctcgacaact ggacgaacgt ctgcgatgcg cagggcgtgt gtgcggcctt cgtgacgagc
     3781 atcctcgtcg gtgttgttgc caccctgatc gcaacggttc tgggcacgct gctggcgatt
     3841 gcgctggccc ggtaccgctt ccgcgggcgt tccgtgatca gtctgctgct gttcctgccg
     3901 atggccactc ctgaggtcgt gctgggtgcg gggcttgcgg cccagttcct gaccgccggg
     3961 gttcccaagg ggctcggcac gatcatcatc gcccacgcgt tgttctgcct cagcttcgtt
     4021 gtggtgaccg tcaaggcgcg cgtcgcgagc ctcgacccgg cgctggagga ggccggccgc
     4081 gatctgtacg ggtctgcgtt ccaggtcttc acccgcatca ctctgcccct gctgatgccg
     4141 ggcatcatcg ccgcggcgct gctgtcattc gcgctgagct tcgacgactt catcatcacg
     4201 cgcttcaaca acggtggagt ggagacgttc cccgtcttcg tctacaccgc cgcggcgcgc
     4261 ggcatgccgc ccgaggccaa cgtgatcgcc tccgcggtct tcatcctcgc gctggtgatc
     4321 gttgtggtcg cccagaccac gtcggcggcg cggcgcaagc gcctcgcgcg cgcccagtag
     4381 cgacatccac gcaatagatc ggcaccatca tgtatgacga ccggcagcgg ctgtctgcgc
     4441 tcactgctgc actcgtgagc ctgtatgcct gaccccgcgg acatcgctgc ccccattccg
     4501 ccaccgcggc ctgagacctt cgtctcggtg gacatcgaga cgtcgggtcc gacgcccggc
     4561 cggtactcga tgctctccgt cggtgcctgc ctggttgatg tccccgagca cggcttctat
     4621 gttgagctca agcccacctc ccggctcgtg gtcgagtccg ctcaggccgt gggtgggctc
     4681 tcgctcgacg atctcgcccg cgacggggtg gatcctgccg aagccatggc gcgcttcgcc
     4741 gactggctcg aggccgaggt gcccgcgggc acggttccgg tcttcgtcgg cttcaacgcc
     4801 gccttcgact ggatgttcgt ggccgactac ttcgaccgat tcctcggccg caaccccttc
     4861 ggtcacgccg cgctcgacat caagagctac acgatgggga tggcggggat cacctgggcg
     4921 cagacgagta tgcgtttcct tgcgccgaag tacctcaatg ggcgaaagtt gacgcacaac
     4981 gccctgggcg acgcccggga tcaggccgag atcttcgccg ctgtgcgggc cgaatcggca
     5041 aggagccagc ctccgggcgt ctgatcgagc acgcggcagc gggaggacga gcatgagcga
     5101 caccagcgac gcagacgtca ggaacatcgt cgagtcggcc cggcgtctgg gcatcgagct
     5161 cgatgaggtg ggaaccatcc agtggctcac tgcgatggcc gcgagtcagg acgacgacca
     5221 gatcgtggtc gacaccgcga gcggcacctt cgggcacaag gtgtcgatgc tcgacttcag
     5281 tccgagggac ctcgaacggt tccgccgcat cggtcagatc gtcgaggtga cgggtcccga
     5341 gggcgtcgcc gagagtgcct tggcactgtc gggttcggcg gcgcagtcga agatccagag
     5401 cttccccggg gactgcgatt acttccagcg gctcaacatc aaggctgaca cgcgcgagca
     5461 ggcgtgcggc atcatggccg gcctgatgag ggacaaggtg ctctcgaccc ttcagggtcc
     5521 cacctaccag ttcctggagg ccaagctcgg ttcgttcgct tgggactgca cccagaacgg
     5581 cgagaagcgc tggaagggca gcccgatcac ctggaaggcg gccgatattg ccgccgggac
     5641 catcgacgtg gtcaaggacg acggcgccgc cgtcaccttg cgctgggacg aggtcgccct
     5701 cgatcccgga tggtgcaaac tcgactgggt cgtcgctgac ccggcgcgca cccagctgtc
     5761 gaacgcgagc aatgtcatcg acgtcacctg ggaggcgccg agcggcgaga tcgtctccct
     5821 cgacggctac ctcgacgcct tcttccagga ggtctacctc gactccgacg atgtgccggt
     5881 cttcgcgaag cttgcgaagt tcgtctcgga tgacgcgctc gacgagtacg tcgaccggct
     5941 cgagggtgag gtgcgcaagt acctcaccga ccacctgaat tacggcaagg cagccaagcg
     6001 catgtacaac gtgttccgct tgtcggggcg tcacctggat gcggccttcg tgcgtgagct
     6061 cttcgacgag ccgaccacga tcctgtatca ggtctggtcg ctcatcgtca cgctcgagaa
     6121 cgcgaccgag ccgggctcgt cgatccccat cgaggccgtg caggcccagg ccgacaccct
     6181 gatcgtcgac gtcgtgaagg cccttgatgg tgatcaggag gacgcgatcg tgcaggcgct
     6241 gctccagctg cgccgcaccc tcgaggatca gaactcgggg gaggcccgca actccgaggt
     6301 cgaggccgct caggcgcagg tggtcaacct catcaacacc ttctaccgcg acaagctcgt
     6361 ggcgatgccg tcgatcaagg agtacatcga cgccgtgcag gccgaggggc cagcgcacta
     6421 gctcgtaggg tcacacgagg gcgatgagct ctccgaccgg ttctccgccg ccgagcacgg
     6481 gctgggtcgt ggcggcgagg acggaggtcg ctcgctcgga gtccacgacg ccctgttcgt
     6541 ccagaagggc gagggctacc gggatcgagg cccgggtcga gccgtcgatg atcttaaggg
     6601 cgacggctgt gccatcgggg gaccccgcga cgaagactcc ctctgcaccg ttcttcgcaa
     6661 tgagtcccag ttcgctcatc acactggcga cggcggggct gtcgagcgcc cagggatccc
     6721 gacggatcgc ccgcgacagt ctctcggcgt ccgcgtcggc accgcgggcc acgagtccgg
     6781 tcgcgcgggc gagcccgcgc agggagacgg cgtgcagcgg cgcgccgcaa ccgtcgatgc
     6841 cggtgtgatc cactggttcc cccgtgtagt cctcgaccgt gcgctggacg atcagctgca
     6901 gggggtgacg cgcagccagg tacgaatcga tgggccatcc gttgcgcaca caggccaaca
     6961 ggaaggctgc gtgcttgccg gagcagttca tcgtgatgcg cgctgggccg gatgcctcgt
     7021 cccgggagtt cgggtcggaa ggccagtcgg ctgggcactg cagtgccgac tcatccaatc
     7081 cggcccggtg gaggatcgat ctcacgacct ctacgtgcgc tgccgtgccc gtgtgactgg
     7141 ctgcggcgag cactagctca gggccgtcga gatcaactcc ggacctcaga acggtgatcg
     7201 cctgaaggaa cttgagagtc gatcgcgggt agatgagcgc gtcgatgtca ccgagggcac
     7261 gcacgacctc accgtcgggc ccgacgacgg cagcggcgcc gaggtggatc gactcgcgca
     7321 tccgcgagcg gtgcactgcg gcaaggggta cgactcccgc cgccgagagg ttgtcgagct
     7381 cggggtcgcc tgccgcgctc cagctcacag gctggcgaac gcctcgtcga tgactcccac
     7441 ggcctccgcg atcagctcgt tgctgatggc gaggctcggc aggaaccgaa gaacgttgcc
     7501 gtaggttccc gccgtcagca gcagcacgcc cttctgcgcg gcgaacgccg agatcgccga
     7561 cacggcggct gcgttgggct cgtgagttcc cggctgcacc agctcgatcg cgatcatggc
     7621 gccgattccg cgcacgtcgc cgatcaccgg gtacttcttc tgcagctcga gcagggcgcc
     7681 cttcagagca ttctcgatgc gcacggcctc cgcgagcagg ttcttctgct cgatctgctc
     7741 gaagacggcg atcgccgcag cggccgacac cgggttgcca ccgaaggtgc cgccgagtcc
     7801 gccgggctgg gccgaatcca tgatctcggc gcgaccggtg acgccggcca gcggcaggcc
     7861 gcccgcgatg cccttggccg agagcaccat gtcgggcacg aggccgaagt gctcgctcgc
     7921 gaagtacttg ccggtgcggg ccatgccgct ctggatctcg tcggcgatga agacgatgcc
     7981 gttggcggtg caccacgcct gaagggcggg caggtagcca tcggcaggca cctggaagcc
     8041 accctcaccc tggatgggct cgacgacgag gcacgcgagg tcggccgcgc ccacggtctt
     8101 ctcgaggtac gagatcgtgc gggccgcggc atccgcaccg ctgagtccat cgtggtacgg
     8161 gtacgagttc ggcgcgcggt agacgtcgcc ggcgaagggc ccgaagccca gtgagtacgg
     8221 agacgccttg aagttcatgg ccatcgtgag gttggtgcga ccgtggtacg cgtgctcgag
     8281 cacggcgacg ccattgcggc ccgtgtactt gcgggcgatc ttgacgccgt tctccacggc
     8341 ctcggcaccc gagttgacga gcaccgtctt cttcgcgaaa tcgccggggg tgtgggcggc
     8401 gaggtactcg gcgacccgca cgtatccctc gtagggcgtg atggtgaaga gggtgtgggt
     8461 gagcttctcg agctgctcgg ttgccccggc caccaccgcg tcatccgtgt gcccgatggt
     8521 ggtcacgccg ataccagcac cgaggtcgat gaactggttg ccgtcgacgt cgaccaggat
     8581 cgcgccgtga gcacgctcga tgtagacggg aagagcggcg gtcacgccca ccggcacgac
     8641 cttgagtcgt cgctcgtgca gggcctgtga cttgggcccg ggaattgccg tgacaacgcg
     8701 gcgttcctga acaacggcag aggtcgtggg cgcaagagtg tcagtcataa tgggctcaag
     8761 tttatccgcc tattgaccgg agcgagccat gcagattgag catcggttcg aggtgcaact
     8821 cgagtggcag ggcaatcgag gaaccggcac cagcaactac cgctcctacg ggcgcgacca
     8881 cgtcatcagt tcgccaggaa atccgcccat ccagggttcg agtgcgcgcg tgttccacgg
     8941 tgatgccgac cgctggaacc ccgaggagat gctcatcgcg tcgctcagcc agtgccacat
     9001 gctcagctat ctgcacgtcg ccaccgcgaa cggaatcgtg gtcgagagct accgggatga
     9061 cgccaccgga acgatcgagc agacggctga cggcggcgga cacttcaccg gagcaaccct
     9121 gcgtcccgtg gtcgagatct cggccgggga acccgagctc gcgcttgcgc tccaccgtga
     9181 ggccaacgag aagtgcttca tcgcatcgtc gatgaacttc gtcgttgcgc acgagccgat
     9241 cattcacgtg gtcgccccgt agctcaccct cgttcgcacc acacgttcag gctcggtgcg
     9301 ccatgatgga tgccttgccc tcaccattcc tgaagaaagt tgccctgtga acgtcactcc
     9361 gcgacgcgct gcactctccc tactcgtcac ggccggactc gtcgcctcgc tcgcggggtg
     9421 caccaccggc tccaccgggt cctcagactg cgctgcaacg ccgtcgggtt ctgtctcgaa
     9481 cgccgtgaag gtctccggcg acttcggcac gaagccggat gtcacgatca acttcccgac
     9541 cgcagccccg gccaccgagc gcacggtcgt gatcaacggt gacggcgaca cagtcgttgc
     9601 gggtgacaac gtcaacgtcg actatgccct cttcgacggt gagaccggcg ttgagctcgc
     9661 tgccaccgag tacaccgagg gttcgaccgt cgagttcccg gttgacgaca cccagttcct
     9721 ggtgggcatc gtgaagacct tggagtgctc caccgtcgga tcgcgcgtgg tcggcgtcat
     9781 ccccgccgcc gactcgttcg gcgaggccgg ctccgaccag ctcggtgtcg ccccgggtga
     9841 cgacatcgtc ttcgtggtcg acgtcgtgtc tatctcggca cccaagattc ccgcgctccc
     9901 caaggccgat ggcgccgacc agccggcgac ccccggcttc ccgaccgtgg tgctggatga
     9961 caccggccgt cccacggtca cgatccccga tgaggcgccc cccactgagc tgaagctcgc
    10021 cgtgctgaag aagggcaccg gggcggtggt agccgatggt gacgacgtca cagtgcacta
    10081 cgtcggcgtc aactggaaca ccaaggtgat cttcgacgag agctgggccc gcggcgagcc
    10141 cgcaaccttc aacaccgggc aggtcatcgc gggcttcacg gcggcgctcg tcggccagaa
    10201 ggtcggttcg caggtcatcg tcatcatccc gccggaccag ggttacggct cggccggcag
    10261 cccgcccgac atcggcggca ccgacaccct ggtcttcgtg atcgacattc tgggcatcgg
    10321 ctgagccagc tagcctgatc acatgcgcag agtgatcatc ctcggttcga ccggttcgat
    10381 cggcactcaa gcgctcgagg tcatcgccgc caacccacac ctcttcgagg tcgtggggtt
    10441 ggcggcgggc agcaaccgcg aggcggtcgg cgcccaggcc gccgagttct cagtggagca
    10501 catcgccttc ggcgccgacg aggcgagcca gctcgtgcgc accgtgcagg cggacgtggt
    10561 gctcaacggc atcacgggtt cggtcgggct cggtcccact ctcgcggtgc tcgagaccga
    10621 ggcggttctt gcgctcgcca acaaggagag cctcatcgtc ggtggtgagc tcgtgacatc
    10681 ccgagtgcgc ccggggcagt tggttcccgt cgactccgag cattcggcga tcgcccaggc
    10741 gctgcgctcg ggcacggcgg acgaggtctc ccgcctggtc ctgaccgctt ccggcggacc
    10801 cttccggggt cgaagccgcg aatcgctgcg agaggtcacc ccccgcgagg cgctcgcgca
    10861 tcccacctgg gacatggggc tcgtggtcac gacgaactcg gcaactctcg tgaacaaggg
    10921 cctcgaggtg atcgaggcgc acctgctgtt cggcgtgccc tacgaccgca tcgaggtcac
    10981 ggttcatccc cagtcgatcg tgcactcgat ggtggagttc gtggatggct cgacgatcgc
    11041 ccaggcgagc ccgcccgaca tgaggcttcc catctcgctc ggactcgact ggcccaaccg
    11101 ggtcgccggg gtgggacgcc ccctcgactg gacggtctcc agtcagtgga ccttcgaacc
    11161 gctcgacacc gaggtcttcc cggcggtcgc actcgccaag cgcgtcggcg aggcgggtcg
    11221 cacctatccc gccgtcttca acgccgcgaa cgagcaggcg gtgctcgcgt tccacgctgg
    11281 gcgcatcggc tacctcgaca tcgtcgacac cgtggagcga gtcgtcgacg ctcacagcgc
    11341 cgacgagttc agcctcgagt cggtcttcga agccgaacgc tgggcgcgcg ccgaggcaga
    11401 caggctgctc acggtttagc ccggctattc ttgcgccgtg gaaactgtcc tgctgtacat
    11461 cctcggcatc ctcatcatcg tcgtggggct cgcattgtcg atcggtctcc acgaactcgg
    11521 ccacctgatc cccgcgaagc gtttcggcgt gcgcgtcggc cagtacatga tcggcttcgg
    11581 tccgacgatc ttctcgagga tgaagggcga gaccgagtac gggctcaagg cgatcccgct
    11641 gggcgggtac atctcgatgt cgggcatgta tgcgccggct cacgagggtg ggcgtggccg
    11701 caccgccacc accagcatgt tcgagaccct ggttcaggat gacaccaagg ccgacgacga
    11761 cgaaggtcgc accttcttcc aactccccgt cttcaagcgc gtgatcatca tgctcggggg
    11821 accggtcatg aacctgctca tcgcggtggt gctgtacacc gtcatcctgt gcggattcgg
    11881 catcgctcag accacgacga cgatcggcag tgtctcgcag tgtgtcgtcg cggcatccag
    11941 tcagagccag gagtgcacga gcgcagaccc agtcgctccc ggtgtcgagg cgggcatcaa
    12001 gcccggcgac cgcatcgtga gcatgaacgg tcaggcgatc ggcacgtggg acgacgccac
    12061 ggcaatcatc cgagcctcgg cgggtaagag tatcgatatc gtcgtcgatc gggccggcgc
    12121 cgaggtcgcg ctgacggcga cccccctgct caccgagcgc tacgtctacg acgacaaggg
    12181 cgctcccgtg aagggtgccg acggcaagca ggtcaccgag gaggtcggat tcctggggat
    12241 cgggtcggcc acggcgatcg tgcagcagcc cgtgaccgcg gtgctgccga ccgtcggtga
    12301 ccgcgtcgtg ggtatcatct cggtgatcac ccagctgccg cagcgcctcg tggatgtcgc
    12361 tgtcgcggcc ttcggtccgg gagatcggga ccccaacggg ctgatcggtg tcgtcggcgt
    12421 cggccgactc gccggtgaga tcaccagcat gagcgacatc ccgatcgcca gtcgcgccgc
    12481 gggaatcatc gaggtggtcg cgtcgctcaa cattgcgctg ttcgtgttca acctgatccc
    12541 gctggtgccg ctcgacggtg gccacatcgc gatcgcgctg atcgacggca tccgacggtt
    12601 cttcgcgcga atcttcaagc gcccggagcc cggccccatc gacgcgaccc gcatcctgcc
    12661 cgtcacgctc gtcgtcacgg tgcttctggg tgcaatgacg ctgcttctcg tctac
//
LOCUS       DAOSOU010000039        12335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5122_length_12335_cov_5.835586, whole genome shotgun sequence.
ACCESSION   DAOSOU010000039 DAOSOU010000000
VERSION     DAOSOU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5122_length_12335_cov_5.835586"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLW15_03520"
     CDS             complement(<1..907)
                     /locus_tag="PLW15_03520"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOT33503.1"
                     /translation="MKNTQSASAMPIHKYKPYHEVLNIDLPDRTWPSKRITHAPRWCA
                     VDLRDGNQALIDPMSPERKRVMFELLVRMGYKEIEVGFPSASQTDFDFVRTLIDEKLI
                     PDDVTIQVLTQAREELINRTYESLIGAKQAIVHLYNSTSVLQRDVVFRTDKQGIIDIA
                     LNGARLCKAAESIAPDVDIYYEYSPESYTGTELEFAVEVCNQVLEVFQPTPDRKVIIN
                     LPATVEMATPNVYADSIEWMSRRINHRENVIISLHPHNDRGTAVAAAELGYMAGADRI
                     EGCLFGNGERTGNVCLVTLGMNLFSQ"
     gene            1141..1785
                     /locus_tag="PLW15_03525"
     CDS             1141..1785
                     /locus_tag="PLW15_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33504.1"
                     /translation="MPMSQRHILTVAAVLTALPLALTGCTTIVATPVTPTTTAPTAEP
                     IEVDKPTNEPIPPASDFTTVYDDSGIVSVNVPSTWTDVVGAQLTNSSGVVFYNVTASP
                     DIAAWQSGWDVPGVSVSSTQDPSIVIEDIINGTIAGPGAECEAPETGDYDDGVYVGTY
                     VYFENCGGVGTDYVSIVAQDADKTHFIICTIQMVSDEDKSTVRDEILNSFWAIY"
     gene            1847..>2208
                     /locus_tag="PLW15_03530"
     CDS             1847..>2208
                     /locus_tag="PLW15_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33505.1"
                     /translation="MRSSRSARSLLPLVLAAVLATTACAGGTSPGAEPTPTSTDVVGQ
                     GTVIQTVDDQAQLCLGAVLESYPPQCTGVPLVGWDWGTVDGQETAAGTTWGTYAVWGV
                     WDGTTLTVSGAIQLALYDP"
     assembly_gap    2209..2308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2309..3635)
                     /locus_tag="PLW15_03535"
     CDS             complement(<2309..3635)
                     /locus_tag="PLW15_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33506.1"
                     /translation="MRRTAREHAPSILVAALSAAFGVALLQGTGALALMIRADDTLGS
                     STAAGIALASVAIVFIAIATYVAAIVTANTFATIIAGRVRTIALLRLLGATAVSQRRS
                     VAREGFVVGCLGALIGAVAGTAITALALGAAALAGWIPSLAYSFVDPVLLVPIVAVIL
                     TTWIASWVGARRVLVVSPLEALGAAQERSVEQVGSRRARNATALVLVIVGFGLLGLGI
                     LVGLVSPAGVLIGMVGGMVSFTGVVLGAHAVMPWTLRAIGRAFGGSASARLAAANAVL
                     YPERSTRTTIGIVIGVTLVTMFGVAGGSFQSLFDRLLSGEDDAAQISLTIAAVVGIFS
                     VLVGFSALIAAVGLVNTLSLGIMQRTRELGLLRALGFTVRQLRAMVIAESGQLVAAAI
                     VVGLLLGGFYGWAGAQSMLALVMGSPAFVVPVVSPVLLAVVVGGAVLLTI"
     gene            complement(3632..4393)
                     /locus_tag="PLW15_03540"
     CDS             complement(3632..4393)
                     /locus_tag="PLW15_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33507.1"
                     /translation="MEITPSDLGLVARVTNLTKSYGAGATTVTALADISLGIRRGEFT
                     AIMGPSGSGKSTLMHIMAGLDSPTGGRVWIGNTEVTNLGDTELTMIRRRRVGFVFQSF
                     NLVPTLDIEGNILLPFELEGRRPSSEEREWIDSLIESLGLTDRLRHRPWELSGGQQQR
                     VAIARALGSRPELVFADEPTGNLDSRTGREVLTLLATASSEYNQSIAMVTHDPVAASY
                     ADRILFLADGVLVDDRGRSTAAEISALMLEMEAAS"
     assembly_gap    4451..4550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4551..5170)
                     /locus_tag="PLW15_03545"
     CDS             complement(<4551..5170)
                     /locus_tag="PLW15_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33508.1"
                     /translation="MSVIRVALVDDQALFRAGIRMLISSQPDLEFVGEAADGREALEV
                     IAAQRPDVVLMDLRMPVMDGIAATEQIMAAPRPPRVIVLTTFDLDEAAARALRAGASG
                     FVLKDSEPEFLLAAIRSVHAGTAVVAASAVRELFEHLGGGRREVAGSLDPLSPREREI
                     FRHAAAGRSNAEIAAAEFLSEATVKTHISRILAKLGLRDRVQLVVFA"
     gene            complement(5167..6297)
                     /locus_tag="PLW15_03550"
     CDS             complement(5167..6297)
                     /locus_tag="PLW15_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT33509.1"
                     /translation="MFRRLSVFQLVVDIAFAAACLAVRFTMTVENPAMGAVVVLMAAA
                     LALRRISPGLALAVAWVGAILQMLAGMTPDASNLAILAVLYTSARYGTRIVRWLGLAS
                     AGLGALVATIYLTSGPGLGLPDSRVLLGAVLTFVGSAAVLGLSWTLGLLVLTWRTSID
                     SRRERGAAQRAVIVEQERNRIARDMHDVVAHSLAVVIAQADGARYASATDPAVAQDAL
                     ATIATTAREALGDVRSLLAQLRHEQGDGPSPTLADLTALVDQLRASGLSVDVSGPIGA
                     NSLPAAQQLALYRIAQEALTNALRHGRPGGRATLSLDVGADAATLTIANTLAEHPRPV
                     GRAGHGITGMTERAHLAGGSLTATAEGDSFVVTARIPVGSRS"
     gene            complement(6369..7271)
                     /gene="era"
                     /locus_tag="PLW15_03555"
     CDS             complement(6369..7271)
                     /gene="era"
                     /locus_tag="PLW15_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT33510.1"
                     /translation="MSKSESQFRAGFVSFVGRPNVGKSTLTNALVGEKVAITSSKPQT
                     TRRAIRGIVHAPDGQLIIVDTPGMHRPRTLLGERLNSIVQTTLGDVDVIGLCLPANEK
                     IGPGDRYINEQLDSFPRAKKVAIVTKIDASSRPGVAEQLQAVSELRDWDAIIPISSFD
                     KVQLDILSTELIKLMPVSEPLYPDDAVTDETTEERISELIREAALEGVSDELPHSIAV
                     TIDDMIESTTKDLLEIYANLFVERDSQKGIIIGRGGERLQDVGARARAEIEKLVGRQV
                     FLSLRVKVAKEWQRDAKQLGRLGF"
     gene            complement(7274..8575)
                     /locus_tag="PLW15_03560"
     CDS             complement(7274..8575)
                     /locus_tag="PLW15_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT33511.1"
                     /translation="MVIGVFFSVAGLLVVFGGLLAAADSALSVLSRNDLVELAASSRS
                     RRSLLAISADPGAHVNALNFMRIVAETTAAVLVTLAFASIFTDWWWTLLASALIMTAA
                     SFVLVGSSPRSVGRVNARPVMRVAAPIVRATRVILGPLADALVTIGNRVTPGRPRTAT
                     FSSEEQLLSMIDEATELDVLEEDDRELIHSIFEFNDTVVREVMIPRTDMVTLDVDATM
                     TASLGLFLSKGVSRMPVIGEDVDEVIGVLYLKDVARVVHEQTLDAATLTAGELSRPAL
                     FVPESQKADSLLRQMQVESSHLAMVVDEYGGIAGLVTLEDLIEELVGDISDEYDRDVA
                     EVEQLGEGRYRVSARLPIDELGDLFGLELEDDEVDSVGGLLTKALGRLPVSGSSAEYS
                     GLILTAERTEGRRKRVSTVLVERDQALIDVQTALDNKETGD"
     gene            complement(8583..9044)
                     /gene="ybeY"
                     /locus_tag="PLW15_03565"
     CDS             complement(8583..9044)
                     /gene="ybeY"
                     /locus_tag="PLW15_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT33512.1"
                     /translation="MSIEINNESGIAVDEQAIQRLAVYALDQLHVHPDAELAIVFVDE
                     AAMEQLHVQWMDEPGPTDVLSFPMDELRPGSEDHLTPAGLLGDIVVCPQVAIAQAETA
                     GHSPLEEMLLLTTHGILHLLGFDHAEPEEEKEMFGIQRDILVGFALAERRR"
     gene            complement(9049..10020)
                     /locus_tag="PLW15_03570"
     CDS             complement(9049..10020)
                     /locus_tag="PLW15_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT33513.1"
                     /translation="MVRLLGPQDRLLSTIEKQHPRVDVLVRGNVITLMGEPAEVHAAQ
                     ELVEELIAMVRNGQELGPVEVSSSARILDSKSGSPAELLSQAILTARGKSIRPKTLGQ
                     KQYVDAIDVNTIVFGIGPAGTGKTYLAMAKAVQALQRKEVDRIILSRPAIEAGERLGF
                     LPGTLTDKIDPYLRPLYDALNEMMDPELVPKLLASGTVEVAPLAYMRGRTLNNSFIVL
                     DEAQNTTPEQMKMFLTRLGFGSRMVITGDVTQVDLPAGASGLQLVTKVLADIDDIHFA
                     RLTSADVVRHTLVGQIVDAYTKYDAEKQLQKFESRESPRKRDRRFGR"
     gene            complement(10076..10426)
                     /locus_tag="PLW15_03575"
     CDS             complement(10076..10426)
                     /locus_tag="PLW15_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOT33514.1"
                     /translation="MTTEPSIFSRIIAREIPADIVFENDAVIAFRDIAPKAPVHILVV
                     PKTQAFANVSELAAGDPALLATVVATAQAVADEYCDGEFRLVFNTGPSAGQTVFHVHA
                     HVLGGTLSEGSLGQ"
     gene            complement(10452..11195)
                     /locus_tag="PLW15_03580"
     CDS             complement(10452..11195)
                     /locus_tag="PLW15_03580"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOT33515.1"
                     /translation="MAHFYLDESLVSAAVGDRISITGAEARHAVTVSRLGVGESISIG
                     NGAGLVVAGVVVSAEHTELVVEVSAVTEVPRATPAVFLAQALAKGDRDELAVQVATEL
                     GVDGVIPWTAARSISRWEGAKVAKGRDRWAAVVREASKQSVRPWLPDVLDLVTTKQLA
                     RLASTDQAEGVRMLVLEPTAATPLSALDFDGRDLVLVVGPEGGITPQELDALSAAGAD
                     IVRLGDEVLRTSTAGPAALAVLAVALGRW"
     gene            complement(11200..12306)
                     /gene="dnaJ"
                     /locus_tag="PLW15_03585"
     CDS             complement(11200..12306)
                     /gene="dnaJ"
                     /locus_tag="PLW15_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT33516.1"
                     /translation="MADHYEVLGVERTATPEEIKRAYRKLARELHPDVNPSAEASERF
                     KEVTHAYDVLSDPRERQQYDLGPQAGFGGGANFGGFSDIFETFFGGGGGRTSGPRSRR
                     ERGQDGLVKVEVDLDEVIFGTKRDLEVTTAVLCETCNGSCCTPGTSPVRCDICGGSGQ
                     IQRTMRSLLGNVVTSNPCGTCQGYGTIIANPCVTCAGKGRVRAQRKIPVDIPAGVDTG
                     LRLQMPGQGEVGPAGGPAGDLYLEIKVRHHETFSRNGDDLLATVEVPMTSAILGTRTT
                     LKGLDGEVLVDIKPGTQSAEVITIKDRGITHLRGQGRGDLRIGIQVVTPSKLSAKEKA
                     LVEQLAAARKQPEPQFAHFQQGLFTKLRDRFMGF"
BASE COUNT         2042 a         4017 c         4126 g         1950 t
ORIGIN      
        1 cctggctgaa caggttcatg cccagggtga ccaggcagac gttgccggtg cgctcgccgt
       61 tgccgaacag gcagccctcg atgcggtcgg cgccggccat gtagccgagc tccgccgccg
      121 ccaccgccgt gccccggtcg ttgtgggggt gcagcgagat gatcacgttc tcgcggtggt
      181 tgatgcgccg cgacatccac tcgatcgagt cggcgtagac attgggcgtg gccatctcga
      241 ccgtcgcggg caggttgatg atcaccttgc ggtcgggcgt cggctggaac acctccagca
      301 cctgattgca cacctcgacg gcgaactcga gctcggtgcc cgtgtagctc tcgggtgagt
      361 actcgtagta gatgtcgacg tcgggggcga tggactccgc cgccttgcac agtcgcgcgc
      421 cgttgagggc gatgtcgatg atgccctgct tgtcggtgcg gaagaccaca tcgcgctgca
      481 gcacgctcgt cgagttgtag aggtgcacga tcgcctgctt ggccccgatg agggactcgt
      541 acgtgcggtt gatgagctcc tcgcgcgcct gcgtgagcac ctggatggtc acgtcgtcgg
      601 ggatgagctt ctcgtcgatg agggtgcgga cgaagtcgaa gtcggtctgg ctggcgctcg
      661 ggaacccgac ctcgatctcc ttgtagccca tgcgcacaag cagctcgaac atgacgcgct
      721 tgcgctccgg gctcatggga tcgatgaggg cctgattgcc gtctcggagg tcgacggcgc
      781 accagcgtgg ggcgtgggtg atgcgcttgc tcggccacgt gcggtcgggc agatcgatgt
      841 tcaatacttc gtgatacggc ttgtacttgt ggatcggcat tgcagaagcc gactgggtgt
      901 tcttcatgag gtcgtgtcct tagggatgtc tgggtcgctg acgagttttg agggtcagac
      961 cgagagtgtt tcttccaaca ccagtctccg cgacgagtga ggcctgaggt taggactcgt
     1021 cgcggcagct aagaagaagc agaccgaaca cacgtcaagg gtagcactgg gagtcgcgcg
     1081 gatgcgtcgc gattcgccat actcgatgaa tcacattcac cagctctgga ggagagcctc
     1141 atgcccatgt cacagcgtca catcctgacc gtcgcggccg ttctgaccgc cctcccactc
     1201 gcactcaccg gctgcacgac gatcgtcgca accccggtca caccgacgac cacggcaccg
     1261 acggccgaac cgatcgaggt cgacaagccc accaatgagc cgatcccgcc cgcctccgac
     1321 ttcaccaccg tgtacgacga cagcggcatc gtgagcgtca acgtcccgtc gacctggacc
     1381 gacgtggtcg gcgcgcaact gaccaacagc tccggcgtgg tgttctacaa cgtgaccgcg
     1441 tctcccgaca tcgccgcctg gcagagcgga tgggacgtgc ccggcgtgag cgtctcgtcg
     1501 acgcaggacc cgagcatcgt gatcgaggac atcatcaacg gcacgatcgc gggtcccggc
     1561 gccgagtgtg aggcgcccga gaccggcgac tacgacgacg gggtgtacgt gggcacctac
     1621 gtctacttcg agaactgtgg cggggtcggc accgactacg tgtccatcgt ggcgcaggac
     1681 gcggacaaga cgcacttcat catctgcacc atccagatgg tctccgacga ggacaagtcg
     1741 acggtgcgtg acgagatcct caactccttc tgggcgatct actgacgact gagcgcatcc
     1801 aaacgttggg tggtggatgc ctcatgccct tggcgatact gagggcatga ggtcatcccg
     1861 ctccgcccga tccctgctcc cgctcgtgct tgccgcggtg ctcgccacca ccgcctgcgc
     1921 gggcggaacg tcaccgggtg ccgaacccac cccgaccagc accgacgtgg tggggcaggg
     1981 cacggtcatc cagacggtcg acgaccaggc gcagctgtgc ctgggtgcgg tactcgagtc
     2041 atacccgcct cagtgcaccg gggtgccact cgtgggctgg gactggggca cggtcgacgg
     2101 gcaggaaacc gccgcaggca cgacctgggg cacctacgcg gtgtggggcg tatgggatgg
     2161 caccaccctc acggtgagtg gcgccatcca gctcgccctg tacgacccnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg atcgtgagca gcacggctcc cccgaccacc
     2341 acggcgagca gcaccggcga caccaccggc accacgaagg cgggcgaccc catgacgagc
     2401 gcgagcatcg actgcgctcc cgcccagccg tagaaaccac cgaggagaag acccacgaca
     2461 atagctgcgg caacaagctg accactttcc gcgatcacca tcgcgcgcag ctgtcgcact
     2521 gtgaaaccca gtgcccggag aaggcccagc tccctggtgc gctgcatgat gccgagcgac
     2581 agggtgttga cgagcccgac cgcggcgatg agagcgctga agcccacgag cacggagaag
     2641 atgccgacga cggcagcgat cgtgaggctg atctgggcag catcatcctc gccggacagc
     2701 agacggtcga acagcgactg gaagctgccg cccgcgacgc cgaacatcgt cacgagcgtc
     2761 acgccgatga cgatgccgat cgtggttcgc gtgctgcgtt ccgggtagag caccgcgttg
     2821 gccgcagcga gccgagcgga tgcactgccg ccgaatgcgc gaccgatcgc ccgcagagtc
     2881 cagggcatga ccgcgtgggc gccgagtacg accccggtga acgacaccat gccgccgacc
     2941 atgccgatca gcactccggc cgggctcacg agaccgacga ggataccgag gccgagcagc
     3001 ccgaagccga cgatgaccag cacgagggca gtggcgtttc tcgcccgccg cgaaccgacc
     3061 tgctcgacgc tgcgctcctg cgcggcgccg agcgcctcga gcggcgagac gacgagcacc
     3121 cgacgagcac ccacccagga tgcgatccag gtggtgagga tgacggcgac gatcggcacg
     3181 agcagcacgg gatccacgaa cgagtaggcg aggctcggga tccagccggc gagggcggcc
     3241 gccccgagcg cgagcgcggt gatcgcggtg ccagctactg caccgatgag agcgccgagg
     3301 catccgacga cgaagccttc gcgggccacg gatcgacgct gactcacagc agtcgcaccg
     3361 agcagccgca gcagggcgat cgtgcgcacc ctgcccgcga tgatcgtggc gaaggtgttg
     3421 gctgtgacga tcgcggcgac gtacgtcgct atcgctatga acacgatcgc caccgacgcg
     3481 agggcgatgc ctgcggccgt gctcgaaccg agcgtgtcgt cggcacggat catgagcgcg
     3541 agggctcccg tgccctgaag gagcgcgacg ccgaatgcgg cgctgagggc agcgacgagg
     3601 atgctcggcg cgtgctcgcg tgcggtgcgc ctcatgacgc cgcctccatc tcgagcatga
     3661 gggcgctgat ctcggcggcg gtcgaccggc ctcgatcatc gacgaggacg ccgtcggcga
     3721 ggaacaggat gcggtccgcg tagctcgccg caacggggtc atgcgtgacc atcgcgatcg
     3781 actggttgta ctcggagctc gccgtcgcaa gcagggtgag cacctcacgc ccggtgcgcg
     3841 agtcgaggtt gccggtgggc tcgtcggcga agaccagctc ggggcgcgaa ccgagggccc
     3901 gcgcgatggc gacccgctgc tgctgaccgc ccgacagctc ccacgggcga tggcgcaggc
     3961 ggtcggtcag gccgagagac tcgatgaggg agtcgatcca ctcccgctcc tcggacgacg
     4021 gtcgtcgacc ctccagctcg aacggcagca ggatgttgcc ctcgatgtcg agcgtcggca
     4081 cgaggttgaa ggactggaac acgaagccca cccgccggcg ccggatcatg gtgagttcgg
     4141 tgtcgcccag gttcgtgacc tcggtgttgc cgatccacac ccggccccct gtcggtgagt
     4201 cgaggcccgc catgatgtgc atgagcgtcg acttgcccga accgctcggg cccatgatcg
     4261 cggtgaactc cccgcgacgg atgcccagac tgatgtcggc gagagcggtc acggtggtgg
     4321 ctcccgcgcc gtaggacttg gtgagattgg tgacccgggc gacgaggccg aggtcggagg
     4381 gggtgatttc catactctcg acgctacgga gggaggcggt gctgccgaat cgcccgcgag
     4441 tcgcgttcga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgaagacca
     4561 cgagctgcac ccggtcgcgc aggccgagct tggcgaggat gcgactgatg tgagtcttca
     4621 ccgttgcttc gctgagaaac tcagcagccg cgatctcggc attgctgcgg ccggccgccg
     4681 catgccggaa gatctcgcgc tccctcgggc tgagcggatc gagcgagccg gcgacctccc
     4741 gacgaccgcc cccgaggtgc tcgaacagct cgcgcacggc agaggccgcc accactgctg
     4801 tgccggcgtg cacactgcgg atggccgcga gcaggaactc gggctcgctg tccttgagca
     4861 cgaaaccgct cgccccggcg cgcagggccc gcgccgcggc ttcgtcgagg tcgaaggtgg
     4921 tcagcacgat cactcggggc gggcgcggtg ccgccatgat ctgctcggtc gcggcgatgc
     4981 cgtccatgac gggcatgcgc aggtccatca gcaccacgtc ggggcgctgg gctgcgatca
     5041 cctcgagcgc ctccctgcca tcggcagcct cgccgacaaa ctcgagatct ggctgcgacg
     5101 agatcagcat gcggatgccc gcgcggaaga gcgcctggtc gtcgaccagc gcaacccgga
     5161 tcacgctcac gagcgggacc cgaccgggat gcgcgcggtc acgacgaacg agtcgccctc
     5221 ggcagtcgcg gtgaggctgc cacccgcgag atgggcgcgc tcggtcattc ccgtgattcc
     5281 gtgaccggcg cgacccacgg ggcgcggatg ctcggccagg gtgttcgcga tggtcagggt
     5341 cgcggcatcc gcgccgacat cgagggacag ggttgcgcgc ccgcccggcc gcccgtggcg
     5401 cagggcattg gtcagtgcct cctgggcgat gcggtagagg gcgagctgct gcgccgccgg
     5461 cagcgagttt gcgccgatcg gcccggacac gtcgaccgac aggccgctcg cccgaagctg
     5521 atcgaccagg gcggtcagat cggccagggt cggactcggc ccgtctccct gctcgtggcg
     5581 cagttgcgcg agaagcgacc gcacatcgcc gagcgcctcg cgggccgtcg tcgcgatcgt
     5641 cgcgagggcg tcctgcgcta cggcagggtc cgtcgccgag gcgtagcgcg cgccgtcggc
     5701 ctgggcgatg accaccgcga gggagtgcgc gacgacgtcg tgcatgtccc gcgcgatgcg
     5761 gttgcgctcc tgctcgacga tcaccgcccg ctgggcggcg ccgcgctccc gtcgactgtc
     5821 gatcgaggtg cgccaggtca gcaccagaag gcccagagtc cagctgaggc cgagcacggc
     5881 ggccgagccg acgaacgtga gaacggcgcc gagcagcact cgcgaatcgg ggagtcccaa
     5941 gcccggtcca ctcgtgaggt agatcgtggc gaccagcgcg ccgagccccg ccgacgcgag
     6001 tccgagccag cgcacgatgc gggtgccgta gcgcgccgag gtgtagagca cggcgagaat
     6061 cgcgagattc gaggcatccg gagtcatccc cgcgagcatc tgcagtattg caccgaccca
     6121 tgcgacggcc agtgcgagac ctggggagat gcggcgcagc gcgagcgccg ccgccatgag
     6181 cacgacgacc gcgcccattg ccgggttctc gacagtcatc gtgaagcgca cagccaggca
     6241 cgcggcggcg aacgcgatgt cgacgaccag ctggaagacg ctcagacgcc ggaacatgcc
     6301 tctacgctat gccgccgcga gtgcccgctc gctccagcgc gtgcagaatc atcccagcga
     6361 tgtatggctc agaaacccag ccggccgagc tgcttggcgt cgcgctgcca ctccttggcg
     6421 accttcacgc gcagcgagag gaacacctgc cggccgacga gcttctcgat ctctgctctc
     6481 gcgcgagcgc cgacatcctg cagtcgctcg ccgccgcgac cgatgatgat gcccttctgg
     6541 ctgtcgcgct cgacgaagag gttggcgtag atctccagca ggtccttggt ggtgctctcg
     6601 atcatgtcgt cgatggtgac ggcaatggaa tgcggtagct cgtcggagac cccctcgagg
     6661 gctgcctcgc ggatgagctc gctgatgcgc tcctcggtgg tctcatcggt cacggcgtca
     6721 tccggataga gcggttccga caccggcatg agcttgatca gttcggttga gaggatgtcg
     6781 agctgcacct tgtcgaaact ggagatcggg atgatcgcat cccagtcgcg cagctcgctg
     6841 accgcctgca gctgctcggc cacaccgggt cgcgatgacg catcgatctt ggtgacgatc
     6901 gccaccttct tcgcccgagg gaacgagtcg agttgctcgt tgatgtagcg atccccgggc
     6961 ccgatcttct cgttggcggg caggcacagt ccgatcacgt cgacatcgcc gagggtggtc
     7021 tgcacgatgc tgttgagacg ctcacccaac agggtgcggg ggcgatgcat gccgggggtg
     7081 tcgacgatga tgagctgccc gtcgggggcg tgcacgatgc cccggatggc acggcgggtc
     7141 gtctgcggct tcgatgaggt gatcgcgacc ttctcgccga ccagcgcatt cgtcagcgtc
     7201 gacttgccca cgttgggacg cccgacgaac gagacgaatc ccgcccggaa ctgcgattca
     7261 gatttcgaca cgctcaatca ccggtttctt tgttgtcgag ggcggtctgc acgtcgatca
     7321 gggcctgatc tcgctcgacg agcactgtgc tgactcgctt gcgccgaccc tcggtgcgct
     7381 cagcagtcag tatgaggccc gagtactcgg cactcgatcc cgaaacgggc aggcgcccga
     7441 gagcctttgt cagcagtccc ccgaccgagt cgacttcgtc gtcttcgagt tcgagtccga
     7501 agaggtcacc cagctcatcg atcggcagcc gcgcgctcac acggtaacga ccctcgccca
     7561 gctgctcgac ctcggcaacg tcgcggtcgt actcgtcgga gatgtcgccg acgagctcct
     7621 cgatgaggtc ctccagggtc acgagcccgg cgatcccgcc gtactcgtcg acgaccatgg
     7681 ccaaatggct ggactcgacc tgcatctggc gcagcagact gtccgccttc tgggactctg
     7741 gcacgaacag cgccgggcgg gagagctctc cggcggtcag cgtcgcggca tcgagggtct
     7801 gctcgtgcac gacgcgggcg acatccttga ggtagagcac accgatgacc tcgtcgacat
     7861 cctcaccgat cacgggcatg cgggagacgc ccttgctgag aaacagaccg agggacgcgg
     7921 tcatggtggc gtcaacgtcg agcgtgacca tgtcggtgcg cgggatcatg acctcgcgca
     7981 cgacggtgtc gttgaactcg aagatcgagt ggatgagttc acggtcatcc tcctcgagca
     8041 cgtcgagttc ggtggcctcg tcgatcatgc tgagcagctg ctcctcgctc gagaacgtcg
     8101 ccgtgcgcgg gcgacccggt gtgacccggt tgccgatggt gacgagcgcg tcggcgaggg
     8161 gaccgaggat gacgcgagtg gcgcgcacga tcggtgctgc cacacgcatc accggtctcg
     8221 cgttgacccg acccacgctg cggggactgg acccgaccag cacgaacgac gccgcggtca
     8281 tgatgagcgc gctggccagc agcgtccacc accagtcggt gaagatactc gcgaaggcca
     8341 gtgtcacgag caccgcagcg gtggtctcgg cgacgatccg catgaagttg agggcgttga
     8401 cgtgagcgcc ggggtcggca gagatggcca gaagggagcg gcgggagcgg ctggatgcgg
     8461 cgagctcgac gagatcgttg cgactcagca ccgacaacgc cgagtcggcg gcggcgagta
     8521 ggccgccgaa gacgaccagc agccctgcga cactgaagaa gacgccgatc accatgatcg
     8581 gtctaccgtc gtcgctcggc cagagcgaat ccgacgagga tgtcgcgctg aatgccgaac
     8641 atctccttct cctcctcggg ctccgcgtga tcgaagccga gcaggtgaag gatgccatgg
     8701 gtcgtcagca gcagcatctc ctcgaggggc gaatgacccg ccgtctccgc ctgggcgatg
     8761 gcgacctgcg ggcagacgac gatgtcgccg agcaacccgg cgggcgtgag gtgatcctcg
     8821 ctgcctgggc gcagctcatc catgggaaag ctcagcacgt cggtggggcc gggctcgtcc
     8881 atccactgca cgtggagctg ttccatcgcg gcctcgtcga cgaagacgat cgcaagctcg
     8941 gcatccgggt gcacgtgcag ttgatcgagt gcgtacacgg cgagacgctg gatggcctgc
     9001 tcgtcgacgg cgatgcccga ttcgttgttg atctcgatac tcatgcggct accgtccgaa
     9061 tcgtctgtcg cgcttgcgcg gggattcccg gctctcgaac ttctgcagct gcttctcggc
     9121 gtcgtacttc gtgtaggcgt cgacgatctg tccgacaagc gtgtggcgca cgacatcggc
     9181 actcgtcaga cgtgcgaagt ggatgtcgtc gatgtcggcg agcaccttcg tgacgagctg
     9241 caggccggat gcgccggcgg gcaggtccac ctgggtgaca tcgccggtga tgaccatcct
     9301 cgagccgaag ccgaggcgag tgaggaacat cttcatctgc tcgggagtgg tgttctgagc
     9361 ctcatcgagc acgatgaagg agttgttgag cgtgcgcccg cgcatgtacg cgagcggcgc
     9421 cacctcgacg gttccgctgg cgagcagctt cggtaccagc tcgggatcca tcatctcgtt
     9481 gagcgcgtcg tagagcggac ggaggtacgg gtcgatcttg tccgtgaggg tgccggggag
     9541 gaatccgagt cgctcgccgg cctcgatcgc cggacggctc aagatgatgc gatcgacctc
     9601 cttgcgctga agcgcctgca cggccttcgc catcgccaga taggtcttgc cggttcctgc
     9661 cggcccgatt ccgaagacga tggtgttgac gtcgatcgcg tcgacgtact gcttttggcc
     9721 gagagtcttc ggccggatgc tcttacctcg cgcggtgagg atcgcctggc tcagcagctc
     9781 ggcggggctg cccgacttgg agtcgagtat gcgcgccgac gacgacacct cgaccgggcc
     9841 gagctcctgg ccgttgcgca ccatcgcgat gagctcctcc accagttcct gtgcggcgtg
     9901 cacctcggcc ggctcgccca tgagggtgat cacattgccg cgcacgagca cgtcgacgcg
     9961 agggtgctgc ttctcgatgg tcgacagcag gcggtcctga ggaccaagca gccgaaccat
    10021 tgcgacaccg tccacctcga gctcgacgcg cgctgcgtca ccggcgggaa cctcgctact
    10081 ggccaagaga gccttccgac agggtgccgc ccaagacgtg ggcgtgcacg tggaacaccg
    10141 tctgccccgc gctcggtccg gtgttgaaga ccagacggaa ctctccgtcg cagtactcat
    10201 cggcgactgc ctgcgccgta gcgacgaccg tcgccagcag ggcggggtca ccggcagcga
    10261 gctcactcac gttggcgaag gcctgggtct tcggcaccac gaggatgtgc acgggagcct
    10321 tcggcgcgat gtcgcggaac gcgatcacgg catcgttctc gaagacgatg tctgctggga
    10381 tctcccgcgc gatgatgcgc gagaagatcg agggctcggt ggtcatggat accagcttaa
    10441 cgtttcccgg atcaccagcg cccgagtgcg accgcgagca cggcgagcgc cgccggtccc
    10501 gctgtggagg tgcgcagcac ctcgtcgccc agccgaacga tgtctgcacc ggcagcgctc
    10561 agggcgtcga gctcctgcgg cgtgatgcca ccctccggcc cgacgacgag aaccaggtcg
    10621 cgcccgtcga agtcgagggc ggacaacggg gtcgctgcgg tcggctcgag cacgagcatc
    10681 cgcaccccct cggcctggtc tgtcgaggcc aggcgcgcga gctgcttggt ggtgacgagg
    10741 tcgagcacat cgggcaacca cggtcgcacc gactgcttcg atgcttcgcg caccacggcg
    10801 gcccaccggt cgcggccctt ggcgaccttg gcgccctccc accgtgagat gctgcgagcg
    10861 gccgtccacg ggatgacgcc atcgacgccg agctcggtgg cgacctgcac cgcgagctca
    10921 tcccggtcgc ccttcgccag ggcttgggcg aggaacacgg ccggcgtcgc gcgcggaacc
    10981 tcggtcacgg cggacacctc cacgaccagc tcggtgtgct ccgcggatac gacgacaccc
    11041 gcgaccacga gcccagcccc gttgccgatc gagatcgact cccccacgcc cagcctgctg
    11101 acggtgaccg cgtgcctggc ctcggcgccg gtgatcgaga tccgatcgcc gacggcggcc
    11161 gacaccagcg attcgtcgag gtagaagtgg gccaagggcc tagaacccca tgaaccggtc
    11221 gcgcagcttg gtgaagaggc cctgctggaa gtgcgcgaac tgcggctccg gctgcttgcg
    11281 agccgccgcg agctgctcga cgagtgcctt ctccttggcg ctcagcttgc tcggtgtcac
    11341 gacctggatt ccgatccgga ggtcgccgcg cccctgcccg cgcaggtggg tgatgccgcg
    11401 atccttgatc gtgatgacct cggcactctg ggttcccggt ttgatgtcga cgagaacttc
    11461 gccgtcgagc cccttgaggg tcgtgcgggt gccgaggatc gccgaggtca tgggaacctc
    11521 caccgtggcg agcaggtcgt cgccgttgcg gctgaacgtc tcgtggtgac gcaccttgat
    11581 ctccagatac aggtcaccag ccggcccgcc cgcgggacca acctcgccct gtccgggcat
    11641 ctggagccgc agaccggtgt cgacgccggc cgggatgtcc acgggaatct tgcgctgagc
    11701 gcgcactcgg cccttgcccg cgcaggtcac gcacgggttg gcgatgatgg tgccgtagcc
    11761 ctggcaggtg ccgcaggggt tggacgtgac gacgttgccc agaaggctgc gcatggtgcg
    11821 ctggatctgg ccggagcccc cgcagatgtc acaccgcacc ggtgaggtgc ccggagtgca
    11881 gcacgaaccg ttgcaggtct cgcagagcac tgccgtcgtg acctcgaggt cgcgtttggt
    11941 gccgaagatg acctcgtcga ggtcgacctc caccttgacg agaccgtcct ggccgcgctc
    12001 acgccgcgag cgcggtccgc tggtgcgacc accgccccca ccgaagaacg tctcgaagat
    12061 gtcgctgaag ccaccgaagt tcgcgccacc gccgaagcct gcctgcgggc cgaggtcgta
    12121 ctgctgacgc tcgcggggat cgctcagcac gtcgtaggcg tgagtgacct ccttgaaacg
    12181 ctcagacgcc tcggcactcg ggttcacgtc ggggtggagt tcacgagcga gcttgcggta
    12241 cgcccgcttg atctcctcag gcgttgccgt gcgctcgacg cccagcacct cgtaatggtc
    12301 agccacaaac agtcctcgtc gttatcaatc ggtgc
//
LOCUS       DAOSOU010000040        12328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5126_length_12328_cov_6.352563, whole genome shotgun sequence.
ACCESSION   DAOSOU010000040 DAOSOU010000000
VERSION     DAOSOU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12328
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_12328_cov_6.352563"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1745
                     /locus_tag="PLW15_03590"
     CDS             18..1745
                     /locus_tag="PLW15_03590"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT33517.1"
                     /translation="MNAIEASIVVLLLARLLPSRVLRSIGDTLKFIVAAAAGSLSIGV
                     FGGLAVWLILGGDPLLTFAEVTPSHFSAIMVILPIVLTRWRDSPKLGIEFAAQVVVLT
                     STVLVVFWPGHFLPLAFLPLPVLTWAAIRFGLPVVTLEVLAVAVASLVMTFMGGGPFA
                     GAGEGRGVFTTTLLQIYFLSYASALLLFGAAAREREQALRRASSSENILRSGLLGAQI
                     GLVIVAQKEGRAEVLEANAAASMVIVTVKGGNGPTLRLSDSYLTDAIEEMWASSRAEW
                     SGEWDAADGGARLNAFITKTHDGNLSIQIIDVTARHKSELATAAALRQESAAAEVLRS
                     LNKRQDEFVASVSHELRTPISSVIGFTDELEETELDDTQRMYTKVIARNGRRLLALVN
                     DLLTLAALDRGESLTNEQPVAFTRLIADGVEDLSAQAKAARVELAFVEGVNCSAETEH
                     PQEVGQVLTNLIGNAVKFTLEGGLVTVMLTERADDVAITVSDTGRGIPPAELESVLER
                     FVRSSSSAGTTGTGLGLPIVKKLVESMGGSILLDSDGVSGTTVTVTLPRAGHSAFGLR
                     GAETEVAPR"
     gene            1755..2204
                     /locus_tag="PLW15_03595"
     CDS             1755..2204
                     /locus_tag="PLW15_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOT33518.1"
                     /translation="MPVNPDLVGRAFAPASPYLVGREKVREFARAVFATSPLNYDPVA
                     ASAAGYADVVAPPTFAVVLQEATLAQLLAEPDAGIDFSRVVHGEQRFSYTRPIVAGDE
                     LTATLSVTSVKTLGGNAMVTAESVIVDPQGEHVVTAISTLVVRGDDA"
     gene            2201..2650
                     /locus_tag="PLW15_03600"
     CDS             2201..2650
                     /locus_tag="PLW15_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT33519.1"
                     /translation="MNLDDLDKLDHRALDHRSIAVGDIVAERTFLLTRDSLVRYAGAS
                     GDFNPIHYRDDVAASVGLPGVLAHGMLTMGAAVQPVVDWLGTSGWVADYGVRFTRPVV
                     VDPLEGASVTVVAKVGVVGEGTARIDLTVTYNNETVLGKAQVQVTFS"
     gene            2650..>3623
                     /locus_tag="PLW15_03605"
     CDS             2650..>3623
                     /locus_tag="PLW15_03605"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT33520.1"
                     /translation="MELSRITTLHVGGPAARYERVTTESQLRDAALSAWSDGDEWLVL
                     GGGSNVVVADQGFEGSVIHVATTGIDRLEDAGGRVRLRVQAGESWDGLVAHAVAHGWS
                     GIEALSGIPGSAGAAPIQNIGAYGQELSGSLVAIQFLDYLTGEVERVNADDLELGYRT
                     SALKRGRQGVVLSIDLELADSPVGTVGYEQLAGALGVEVGARVPIADIRSQVLSLRAS
                     KGMVLDPADPDTVSCGSFFTNPIVSERFAHGLPPETPRWLQDDDTSVKLSAAWLIEQA
                     GIGRGFALPGSRAAISSKHTLAITNRGGASAEQVAELSRYVRAQVANR"
     assembly_gap    3624..3723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3891..4508)
                     /locus_tag="PLW15_03610"
     CDS             complement(3891..4508)
                     /locus_tag="PLW15_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33521.1"
                     /translation="MIRVLVADDHPIVRGGIVALLESADDLSVVGEASTGLQAVELAL
                     EHEPDIVLMDLQMPELSGAEATARILEQRPGIRVIVLTTYESDSAILQAIEAGASGYL
                     LKAAPAGEILAGIRSVVAGDVALAPSIAAMLVNRVKAPAGPTLSARETEVLALVAQGK
                     SNPAIAQALFVSEATIKTHLLHVFEKLNVSDRTRAVTKAMELGLL"
     gene            complement(4505..5644)
                     /locus_tag="PLW15_03615"
     CDS             complement(4505..5644)
                     /locus_tag="PLW15_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT33522.1"
                     /translation="MESSRWWHVAVLGIAAVLSGLIIWGDPSTIGLVGGLTAIAVFVA
                     AWFVVGRRATRSQRAGIAFVVITIVVAAVGTAFSPNLATIQCIAYPIIWVLIDRTEAA
                     ILANIALATGVAVGLYLSTGSALQALLIQGISVTFSIALGLWITSIAVRSHERQRLLD
                     ELRATQDELAMVSRDAGVSSERERLAREIHDTIAQDLTGLVLTTQRARRELADGALPA
                     LDEQLAILEENARSALAETRALVAATAPIGLATGGIGDALDRLGARFARETGLAVTVS
                     ATGLPALDRDTEVVLLRCAQEGLANVRKHARAGAASLTVAADDDEVTLEVRDDGRGFD
                     PGTVAAGFGLSGMRERLALVDGRLAVASGATGTTLTVTLPRGEQE"
     gene            complement(5645..6478)
                     /locus_tag="PLW15_03620"
     CDS             complement(5645..6478)
                     /locus_tag="PLW15_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33523.1"
                     /translation="MTTIDIPRLPAGRTLRLGVSRIGYEVKTYFRQGDSVFFTFLFPL
                     VMLTIFSVAFSEQSFGPPGEEISAAAFYLPGMLAAGLLLSGLQNMAIDIANEKSDGTL
                     KRFAGTPLPVVSYFIGKIGQVLVTGLMQAGLLLLVAGLVFRVELPTEPEKWFTFAWVF
                     VLGVSTSAVLGIALSGLPRSGKSATAVIIPIALVLQFISGVYLGFSQLPEWLQNIASV
                     FPLKWMAQGMREVFLPASFEVLEQGESWNLPGVAIVCAIWLVVGLIVTRFTFRWIRKD
                     S"
     gene            complement(6475..7356)
                     /locus_tag="PLW15_03625"
     CDS             complement(6475..7356)
                     /locus_tag="PLW15_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33524.1"
                     /translation="MTSVVSVRDLRKSYGNFTALDGVSFDIEAGETFALLGPNGAGKS
                     TTIEILEGYRDRTSGEASVLGVDPGKGGLDWKAQLGIVLQSTGESGNITVREQLTHFA
                     GFYPNARDVDEVIAAVGLEAKQKAMIRKLSGGQRRRVDVALGIIGRPELLFLDEPTTG
                     FDPEARRQFWDLIRSLKAEGTTILLTTHYLDEAAQLGDRAGIIAGGRLIDIGRIDEIG
                     GADARVPLVRWLENGQQREERTTTPGALVARLGEPEGLEVIRPSLEDIYLTLVGGYEA
                     GEVSTSSTTGTTEGAAL"
     gene            complement(7448..8644)
                     /locus_tag="PLW15_03630"
     CDS             complement(7448..8644)
                     /locus_tag="PLW15_03630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT33525.1"
                     /translation="MTLPRISRRIAAIAESATLKVDAKAKALQAEGRPVISYAAGEPD
                     FATPSNIVEAASRAVLDPKNYKYTPAAGLPELREAIAQKTLRDSGLEVAPSQVIVTNG
                     GKQAVYQAFATLLDDGDEVLLPTPYWTTYPEAIKLAGGVPVDVFAGADQNYLVTVEQL
                     EAARTDKTKVLLFVSPSNPTGAVYSPEQTKAIGEWAEAHGLWVISDEIYQNLVYDGVR
                     AVSIVEAVPALADRTILVNGVAKSYAMTGWRLGWMVGPADAIKAAANLQSHLTSNVSN
                     ITQRAAIEALTGPQDAVEAMRVSFEKRRNTIVAELSKISGLVCPVPEGAFYVYPDVTG
                     LLNRTWGGVTPTTSLELADLILDQAEVAVVPGEAFGPSGYLRLSYALGDAPLLEGVQR
                     LQRLFA"
     gene            8840..8912
                     /locus_tag="PLW15_03635"
     tRNA            8840..8912
                     /locus_tag="PLW15_03635"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8873..8875,aa:Trp,seq:cca)
     gene            8943..9227
                     /gene="secE"
                     /locus_tag="PLW15_03640"
     CDS             8943..9227
                     /gene="secE"
                     /locus_tag="PLW15_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT33526.1"
                     /translation="MARKVIDEPSEDIVANAKKERAQRRGPFGRVALFIRQVVAELRK
                     VVTPTRKELFSYTGVVLVFVVIMMALVYGLDFVFSLLVNFLFGDPTLGNG"
     gene            9283..10281
                     /gene="nusG"
                     /locus_tag="PLW15_03645"
     CDS             9283..10281
                     /gene="nusG"
                     /locus_tag="PLW15_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT33527.1"
                     /translation="MSENHRDDFEIATAAEQSAEDDEAQTGNMLAEADKSEDAAESQA
                     MHLVDDEDEGDLAGLLEALDAAIDPEADALVDDALEVDSLDEADAALAAAEDEAEIEA
                     AEAALEEPDVTPYDGPELGDDEASDADPYESFRVELRGKPGKWYVIHSYAGFEKRVKQ
                     NIENRKVSMAMEDYVFQVEVPMEDVVEIKNGQRKLVNRVRIPGYVLVRMDLNEDSWSV
                     VRHTPGVTGFVGNSHNPRPLRFEEAFNMLKSLVQIVEAPAAKGAGGKGKAAARVIPAE
                     IDFEIGETITIKEGSFAGLPGSISEIKAESGKLIVLVSLFERETPVELSFDQVTKL"
     gene            10502..10933
                     /gene="rplK"
                     /locus_tag="PLW15_03650"
     CDS             10502..10933
                     /gene="rplK"
                     /locus_tag="PLW15_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009233246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT33528.1"
                     /translation="MAPKKKVTGLIKLQIQAGAANPAPPIGPALGQHGVNIMEFCKAY
                     NAATESQRGNVIPVEITVYEDRSFTFILKTPPAAELIKKAAGVQKGSSTPHTVKVAKL
                     TKEQVRSIAEQKMVDLNANDIEGAEKIIAGTARSMGITVEA"
     gene            10936..11625
                     /gene="rplA"
                     /locus_tag="PLW15_03655"
     CDS             10936..11625
                     /gene="rplA"
                     /locus_tag="PLW15_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT33529.1"
                     /translation="MATKSKAYRAAAEKIEEGKFYSPSEAVAVAKVTGSKKFDSTVEV
                     ALKLGVDPRKADQMVRGTVILPHGTGKTARVIVFATGPAAEAAIAAGADEVGGAELID
                     KVAAGYTAFDAAVSTPELMGQVGRLGKVLGPRGLMPNPKTGTVTQDTGKAVSDIKGGK
                     IEFRVDKHANVHFVVGKAGFTEEQLSENIKAAIDEVVRSKPSSSKGRYITKGTVSTTF
                     GPGIPLDVNAL"
     gene            11689..12213
                     /locus_tag="PLW15_03660"
     CDS             11689..12213
                     /locus_tag="PLW15_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33530.1"
                     /translation="MHIRPATPDDAALLHELAAATFPLACPPDTLPASIESFIATNLS
                     VDSFTAYLSDPARLLFVGEVDGVASGYTMVVFGEPGDADVAASVTARPTAELSKCYAL
                     PSAHGTGLAAALVTASVQAARDRGARSVWLGVNQQNARANRFYEKQGFAQVGTKRFLV
                     GERYEDDFVRSLEL"
BASE COUNT         2039 a         4045 c         4123 g         2021 t
ORIGIN      
        1 ccgtcgtttt cggcgtcatc aacgcgatcg aggcgtccat tgtcgtgctg ctgctcgctc
       61 gtctcctgcc gtcacgagtc ctgcgctcca taggggacac tctgaagttc attgttgcgg
      121 ccgccgcagg gagtctgtcc ataggcgtct tcggtgggct agcagtctgg ctcatcctcg
      181 gtggagaccc ccttctcacg ttcgctgagg tcacgccatc gcacttctcc gcgatcatgg
      241 tcatcctccc catcgtgctc acgagatggc gggactctcc caagctcggg atcgagtttg
      301 ctgcgcaggt tgtcgtactc acgtccactg tgctggttgt cttctggcct gggcacttcc
      361 tgcccctggc gttcctgccg cttccggtac tcacctgggc cgcgattcga ttcggacttc
      421 ctgtggtgac tctcgaagtt cttgccgtag cggtcgcgag tctggtcatg acattcatgg
      481 gtggtgggcc attcgcgggt gccggggagg ggcgaggcgt gtttaccacc accctcctgc
      541 agatctactt cctgtcctac gcgagtgcct tgctgttgtt cggtgctgct gccagagagc
      601 gggaacaggc acttcgccgc gcaagttcta gcgagaacat cctcagaagt ggtctgctcg
      661 gggcgcagat cgggctggtg atcgtcgctc agaaagaggg gcgagccgag gtactggagg
      721 ccaatgcggc cgcatcaatg gtgatcgtca cggtcaaggg cggcaatggg ccgacgctgc
      781 gattgtccga ctcctacttg acggacgcca tcgaggagat gtgggcgtca agccgtgctg
      841 agtggtcggg tgagtgggac gctgcagacg gtggcgcccg cctcaacgca ttcatcacga
      901 agacgcacga tgggaatctc agcatccaga tcatcgatgt gacggcccgt cacaaatcgg
      961 aactggctac cgcggcggca ttgcgtcagg agtccgcagc ggcggaggtc ctgcgaagcc
     1021 tcaataagcg tcaggacgag ttcgtcgcgt cagtcagtca cgagctacgc acacccatct
     1081 ccagcgtgat tgggttcaca gatgagctgg aggagaccga gctcgacgac acgcagcgca
     1141 tgtacaccaa ggtcatcgcg cgcaacgggc gccgattgct agccctggtt aacgacctcc
     1201 ttaccttggc tgcgctcgat cgtggagagt cactgaccaa cgagcagccc gtggcgttta
     1261 cccgcctgat tgctgatggt gtcgaagacc tatcggcaca ggcgaaggcg gcacgcgtcg
     1321 aactcgcctt cgtcgagggg gtaaattgct ctgcggaaac ggaacatccg caagaggtcg
     1381 gtcaagtgct caccaacctc attggcaatg ctgtgaagtt cactcttgag ggcggccttg
     1441 tcacagtgat gctcacagag cgcgcagatg atgtcgcaat taccgtgtcg gacacggggc
     1501 gtgggattcc ccccgccgaa ctcgaatccg tactggagag gtttgtgcga tcgagttctt
     1561 ccgcgggtac caccggaact ggtctaggtc taccgatcgt caagaagctg gtcgagagca
     1621 tgggcggaag catcctcctc gactccgacg gcgtatcggg gaccaccgtc accgttaccc
     1681 tgccgcgggc tgggcacagc gccttcggac tgcgtggcgc agagaccgag gtggcacccc
     1741 gttaagctgg gaccgtgcca gtgaaccccg acctcgtggg acgcgccttc gcgccggcgt
     1801 ccccctacct cgtggggcgt gagaaggtgc gcgagttcgc gcgagccgtc ttcgccacga
     1861 gcccgctcaa ctacgacccg gtcgcggcat ccgctgccgg gtatgccgac gtggtcgctc
     1921 cgcccacctt cgcggtggtg ctgcaggagg cgaccctcgc gcagctgctg gccgaaccgg
     1981 atgccggcat cgacttctcg agggtcgtgc acggcgagca gcgcttcagc tacacccgcc
     2041 cgatcgtggc cggcgacgag ttgaccgcca ccctcagcgt gaccagcgtg aagaccctcg
     2101 gcggcaacgc gatggtcacc gccgagtcgg tgatcgtcga cccgcagggt gagcacgtgg
     2161 tcaccgcgat ctcgacgctc gtcgtgaggg gggatgacgc gtgaatctcg acgatctcga
     2221 caagctcgat caccgcgcgc tcgatcaccg ctcgatcgcc gtcggcgaca tcgtcgccga
     2281 gcgcaccttc ttgctcaccc gcgactcgct cgtgcgctac gcgggtgcct cgggtgactt
     2341 caacccgatc cactaccgcg atgacgtggc ggcctcggtc ggcctgccgg gcgtgctcgc
     2401 ccacggcatg ctgaccatgg gcgccgccgt gcagccggtc gtcgactggc tcggaacgag
     2461 cggctgggtg gccgactacg gggtgcgctt cacgcgcccg gtcgtcgtcg acccgctcga
     2521 gggcgcgagc gtgacggtcg tcgccaaggt gggtgtggtt ggcgagggca ccgcccgcat
     2581 cgacctcacc gtgacctaca acaacgagac cgtgctgggc aaggcgcagg tgcaggtcac
     2641 cttcagctga tggagctctc gcgtatcacc acgctgcacg tcggcggccc cgccgcccgg
     2701 tacgagcgcg tcaccaccga gtcgcagctg cgggatgcgg cgttgtcggc ctggtccgac
     2761 ggcgacgagt ggcttgtgct cggcggcggc agcaacgtgg tggttgcgga tcaggggttc
     2821 gagggcagcg tcatccatgt cgcgacgacg ggaatcgacc gactcgagga tgccggcggc
     2881 cgggtgcgcc tgcgcgtgca ggcgggcgaa tcctgggatg gtctcgtagc ccacgcggtg
     2941 gcgcacggct ggagcgggat cgaggcgctg agtggtatcc cgggcagcgc gggtgccgcg
     3001 ccgatccaga acatcggcgc ctacgggcag gagttgtcgg gctcgctggt tgcgatccag
     3061 tttctggact acctcacggg ggaggtcgag cgtgtcaacg ccgacgatct ggagctcggc
     3121 tatcgcacct cggccctcaa gcgcgggcgg cagggggtcg tgctgtcgat cgacctcgag
     3181 ctggccgact cgcccgtcgg caccgtcggc tacgaacagc tcgctggtgc cctcggggtc
     3241 gaggtcgggg cgcgcgtgcc gatcgcggac atccgctcac aggtgctgtc actgcgcgcg
     3301 agcaagggca tggtgctcga ccccgcagac cccgacacag tcagttgcgg gtctttcttc
     3361 accaacccga tcgtgagcga gcgcttcgcg cacgggttgc cgccggagac gccgcgctgg
     3421 ctgcaggacg acgacacttc ggtcaagctc agcgccgcct ggctgatcga gcaggccgga
     3481 atcggccgcg gtttcgcgct gcctggctcg cgcgcggcga tctcgtcgaa gcacaccctg
     3541 gcgatcacca atcgcggcgg cgcgagcgcg gagcaggttg ccgagctgtc gcgctacgtg
     3601 cgcgcgcagg tcgcgaaccg cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnntcggtgg gggatgcggc gattcgacct tgcccttctg gcaccttctg ttgtgcagga
     3781 gtcgcgagca tgtgtcgtga ctcctgcaca acagaagtct ccagcgccta ggacgaggct
     3841 gagagccgat gcctcgaggg tccgcacggc agaagagctc ctgctcgccg ctaaagcagc
     3901 cccagctcca tcgccttcgt caccgcgcgg gtgcggtcgc tcacgttcag cttctcgaac
     3961 acgtgcagca ggtgcgtctt gatcgtcgcc tcgctcacga acagcgcctg cgcgatcgcc
     4021 gggttgctct tgccctgcgc gaccagcgcg agcacctcgg tctcgcgggc gctcagcgtc
     4081 ggccccgcgg gggccttcac gcgattgacg agcatggccg cgatcgaggg agcgagcgcg
     4141 acatcccccg ccaccaccga gcggatgccc gccagaatct cccccgccgg tgcggccttg
     4201 agcaggtacc cgctcgcacc cgcctcgatc gcctgcagaa tcgcggagtc gctctcgtag
     4261 gtggtcagca cgatcacgcg gatgcccgga cgttgctcga ggatgcgcgc agtcgcctcg
     4321 gcgccgctca gctccggcat ctgcaggtcc atgagcacga tgtcgggttc gtgctccagc
     4381 gcgagctcga cggcctgaag ccccgtgctc gcctcaccga cgacactcag gtcgtcggcc
     4441 gactccagca gcgcgacgat tccgccccgc acgatcggat ggtcatcggc gaccagtacc
     4501 cggatcattc ctgctcccct ctcggcagag tgacggtcag tgtcgtgccc gttgcaccgg
     4561 aggcgaccgc gaggcgtccg tcgacgagcg cgaggcgctc acgcatgccg ctcagtccga
     4621 acccggccgc cacggtgccc ggatcgaaac cgcggccgtc gtcacgcacc tccagggtca
     4681 cctcgtcgtc atccgctgcg acagtgaggg atgccgcccc ggcccgagca tgcttgcgca
     4741 cgttagcgag tccctcctgc gcacaccgca gcagtacgac ctcggtgtca cggtcgagcg
     4801 cgggcaaccc ggtggcgctc acggtcacgg cgagtcccgt ctcccgggcg aaccgtgcac
     4861 ccagccggtc gagcgcgtcg ccgatgccac ctgtggccag gccgatcggg gcggtggccg
     4921 caaccagtgc gcgggtctcg gcgagggcgc ttcgggcgtt ctcctcgagg atcgcgagct
     4981 gctcgtcgag ggcaggcagc gccccgtccg cgagctcgcg gcgggcgcgc tgggtggtga
     5041 gcaccagccc ggtgaggtcc tgagcgatgg tgtcgtggat ctcgcgggcg agacgttccc
     5101 gctcgctcga cacaccggca tcccgactca ccatcgccag ctcgtcctgg gtggcgcgca
     5161 gctcatcgag cagacgctga cgctcgtgac tgcgcacggc gatgctggtg atccagaggc
     5221 cgagcgcaat gctgaaggtc accgagattc cctggatgag cagcgcctgc aacgccgatc
     5281 ccgtgctgag gtacagccca acggccacac ccgtggcgag ggcgatgttg gcgaggatcg
     5341 ctgcctcggt gcgatcgatg agcacccaga tgatcgggta cgcgatgcac tggatggtcg
     5401 cgaggttggg cgagaacgcc gtgccgacgg ccgctacgac gatggtgatc acgacgaacg
     5461 cgatccccgc ccgctggctg cgggtcgcgc ggcggccgac cacgaaccag gcggcgacga
     5521 acacggcgat cgcggtgagc ccgccgacga gtccgatcgt cgacgggtcg ccccagatga
     5581 tgaggccgga cagcaccgcc gcgataccca gtaccgcgac gtgccaccac cggctggact
     5641 ccatctagct atccttgcgg atccagcgga acgtgaagcg ggtcacgatg agaccgacca
     5701 ccagccagat cgcgcagacg atcgcgacgc ccggcagatt ccagctctca ccctgctcca
     5761 gcacctcgaa gctcgccggc aggaacacct cgcgcatgcc ctgcgccatc cacttcaggg
     5821 ggaagacgct cgcgatgttc tgcagccact ccggcagctg cgagaagccg aggtagacgc
     5881 ccgagatgaa ctgcagtacg agggcgatag ggatgatcac ggcggtggcg ctcttgccgc
     5941 tgcggggcag gccgctcagc gcgatgccga gcaccgccga ggtggagacg ccgaggacga
     6001 acacccaggc gaacgtgaac cacttctcgg gctcggtcgg cagctcgacc cggaagacca
     6061 ggccggccac gagcagcagc aggccggcct gcatgaggcc cgttaccagc acctgaccga
     6121 tcttgccgat gaagtagctc accaccggca ggggtgtgcc ggcgaagcgc ttcagcgtgc
     6181 cgtcgctctt ctcgttggcg atgtcgatgg ccatgttctg caggccactg agcagaaggc
     6241 ccgcagcgag catcccgggc aggtaaaacg ctgcggcgct gatctcctca ccgggagggc
     6301 cgaagctctg ctcgctgaac gcgaccgaga agatcgtgag catcacgagc gggaacagga
     6361 acgtgaagaa caccgagtcg ccctggcgga agtaggtctt cacctcgtac ccgatgcggc
     6421 tcacgccgag gcgcagcgtg cggcccgcgg gcaggcgggg gatgtcgatg gtggtcacag
     6481 tgctgctcct tcggtggtgc cggtggtcga gcttgtcgag acctccccgg cctcgtaccc
     6541 gcccacgagt gtcaggtaga tgtcctcgag gctcgggcgg atgacctcga gtccctcggg
     6601 ctcgccgagg cgagccacga gcgcgcccgg tgtggttgtg cgctcctcgc gctgctgccc
     6661 gttctcgagc cagcgcacga gtggcacacg ggcatccgcg ccaccgatct cgtcgatgcg
     6721 gccgatgtcg atgaggcgac caccggcgat gatgcccgcg cggtcgccca gttgggcggc
     6781 ctcgtcgagg tagtgggtgg tgagcaggat cgtggtgccc tccgctttga gggagcggat
     6841 gaggtcccag aactggcggc gtgcctcggg gtcgaagccc gtggtgggtt cgtccaggaa
     6901 gagcagctcg gggcgtccga tgatgccgag tgcgacatcc acgcggcggc gctggccacc
     6961 cgacagcttg cggatcatcg ccttctgctt ggcctcgagc ccgaccgcag cgatcacctc
     7021 gtcgacgtcg cgggcgttcg ggtagaagcc cgcgaagtgc gtcagctgct cgcgcacggt
     7081 gatgttgccg ctctcgccgg tcgactgcag cacgatgccg agctgcgcct tccagtcgag
     7141 gccgcccttg ccggggtcga ccccgagcac ggatgcctcg ccgctcgtgc ggtcgcggta
     7201 gccctcgaga atctcgatcg tcgtgctctt gccggcgccg ttcgggccga gcagcgcgaa
     7261 cgtctcgccg gcctcgatgt cgaaactcac gccgtcgagc gccgtgaagt tgccgtagct
     7321 cttgcgcagg tcgcgcacac tcaccactga tgtcatgagt ccatcgtggc gggtgccgcg
     7381 ctgccgcggc agcggtgcac cggatgaacc tgccatccac cgatcggtgg atgccgcggg
     7441 gctgccccta cgcgaacagg cgctgcaggc gctgcacgcc ctcgagcagc ggggcgtcgc
     7501 cgagggcgta cgacaggcgg aggtatcccg agggtccgaa cgcctcgccc ggtaccacgg
     7561 cgacctcggc ctggtccagg atgaggtcgg cgagctcgag cgacgtggtg ggcgtgacgc
     7621 cgccccaggt gcggttcagc aggcccgtga catccgggta gacgtagaag gcgccctcag
     7681 gaacaggaca caccagaccg gagatcttgg agagctcggc gacgatcgtg ttgcggcgct
     7741 tctcgaagga gacgcgcatc gcctcgacgg cgtcctgcgg gccagtgagg gcctcgatcg
     7801 cggcgcgctg cgtgatgttc gacacgttgc tcgtcaggtg cgactgcagg ttggcggcgg
     7861 ccttgatggc gtcggcggga ccgaccatcc agccgaggcg ccatccggtc atcgcgtagc
     7921 tcttcgcgac accgttgacg aggatggtgc ggtcagcgag ggccggcacc gcctccacga
     7981 tcgagaccgc acgcacgccg tcatagacca ggttctggta gatctcgtcg gagatcaccc
     8041 agagtccgtg cgcctcggcc cactcgccga tcgccttggt ctgctccggt gagtacaccg
     8101 cgccggtcgg gttcgacggc gagacgaaca gcagcacctt cgtcttgtcg gtgcgcgccg
     8161 cctcgagttg ctcgacggtc accagatagt tctggtcggc acccgcgaag acgtcgacgg
     8221 gtacgccgcc ggcgagtttg atcgcctccg ggtaggtggt ccagtacggg gtcggcagca
     8281 gtacctcgtc gccgtcatcc agaagggtgg cgaaggcctg gtacacggcc tgcttgccgc
     8341 cgttggtgac gatcacctgg ctgggcgcca cctcgagtcc gctgtcgcgc agggtcttct
     8401 gtgcgatcgc ctcgcgcagt tcggggagcc ccgcggcggg cgtgtacttg tagttcttcg
     8461 ggtcgagaac ggcccgggat gccgcctcga cgatgttgct cggagtggcg aagtcgggct
     8521 cgcccgccgc gtagctgatg acgggccgtc cctcggcctg cagggccttg gccttcgcgt
     8581 cgaccttgag ggtcgccgac tcggcgatgg cggcgatccg gcgggagatt cggggaagag
     8641 tcacggcacc aattctaggc gtgcacggca agagtggccg gagcggttcc gggggtgcag
     8701 cagccactct cctgccgacg aacgaggctc ggcacaattc gtctggccag ttcgatgccg
     8761 tacacttgtc gcgtggtagt tgaaatctgc catgctttcg cgcgcccaga cgccgcggcc
     8821 gcaggagatt tcagcccgaa gggcggtggc tcaattggta gagcagcggt ctccaaaacc
     8881 gcaggttgca ggttcgagtc ctgtccgccc tgcagcaagc cgaaacatcg gaaggtaaca
     8941 acgtggccag aaaagtgatc gacgagccca gcgaggacat cgtcgccaac gccaagaagg
     9001 agcgcgccca gcggcgcggt ccgttcgggc gtgtggcgct gttcatccgc caggtcgtcg
     9061 ccgagctgcg caaggtcgtc acgcccaccc gcaaggagct gttcagctac acgggcgtcg
     9121 tgctggtctt cgtggtgatc atgatggccc tcgtctacgg tctggacttc gtgttcagcc
     9181 tgctcgtcaa cttcctgttc ggtgacccga cgctcggcaa cggctaacca accctgaacg
     9241 cggcggtcat caccgccagg tatcgcaacg aaaagagatc cagtgtctga gaaccaccgc
     9301 gacgattttg agatcgcgac ggccgccgag cagtccgccg aggacgacga ggcacagacg
     9361 ggcaacatgc tcgccgaggc cgacaagtcg gaggacgccg ccgagagcca ggccatgcac
     9421 ctggtcgacg acgaggatga gggtgacctg gccggtctgc tcgaggcact cgatgccgcc
     9481 atcgaccctg aggccgacgc gctcgtagac gatgccctcg aagtcgattc cctggacgag
     9541 gcggatgccg cactcgccgc cgccgaggac gaggccgaga tcgaggcggc tgaggccgct
     9601 ctcgaggagc ccgacgtcac cccctacgac ggacccgaac tcggcgacga cgaggcatct
     9661 gacgctgacc cctacgagtc gttccgcgtt gagctgcgcg gcaagccggg caagtggtac
     9721 gtcattcact cctacgcggg cttcgagaag cgcgtgaagc agaacatcga gaaccgcaag
     9781 gtctccatgg ccatggagga ttacgtcttc caggtcgagg ttcccatgga ggacgtggtc
     9841 gagatcaaga acggccagcg caagctggtc aaccgcgtgc gcatcccggg ctacgtgctc
     9901 gtgcgcatgg acctcaacga ggacagctgg tcggtcgttc gtcacacccc cggtgtgacc
     9961 ggcttcgtgg gcaactcgca caacccccgc ccccttcggt tcgaggaggc cttcaacatg
    10021 ctgaagagcc tcgttcagat cgtcgaggct ccggcggcca agggcgccgg cggcaagggc
    10081 aaggctgcgg cgcgcgtcat cccggcggag atcgacttcg agatcggcga gaccatcacg
    10141 atcaaggagg gctcgttcgc gggtcttccg gggtcgatca gcgagatcaa ggcggagagc
    10201 ggcaagctca tcgtgctcgt ctcgctcttc gagcgcgaga ccccggtcga gctgtcgttc
    10261 gatcaggtca ccaagctgta gcgctggttg agtagcaccc gaagggtgcg tatcgaaacc
    10321 cttgggttga gtgcgacgaa gtcgcgtatc gaaaccgagc ccgttttcgg gtaaagttac
    10381 acggttgcgt ggtttcgata cgccgcttcg cggctactca accaacgtca cctcaccact
    10441 gccggcatca ggccggttcg tggcagcgcg tccatccgga cgcacgacac aaaggaaaac
    10501 aatggcaccg aagaagaagg ttacgggtct gatcaagctt cagatccagg ccggcgccgc
    10561 caaccccgca ccgcccatcg ggcctgcgct gggtcagcac ggcgtgaaca tcatggagtt
    10621 ctgcaaggcg tacaacgcgg cgaccgagtc gcagcgcggc aacgtcatcc cggtggagat
    10681 caccgtctac gaggaccgtt cgttcacgtt catcctcaag accccgcctg ccgccgagct
    10741 catcaagaag gccgccggcg tgcagaaggg gtcgtcgacc ccgcacacgg tcaaggtcgc
    10801 gaagctcacc aaggagcagg tgcgctcgat cgccgagcag aagatggttg acctcaacgc
    10861 caacgacatc gagggcgcag agaagatcat cgcgggcacc gcccgttcca tgggaatcac
    10921 ggtggaggcg taatcatggc aaccaagtcc aaggcatacc gcgccgcagc ggagaagatc
    10981 gaagagggca agttctacag cccgtccgag gccgtcgctg tcgcgaaggt caccggctcg
    11041 aagaagttcg actcgaccgt cgaggtcgcc ctcaagctgg gtgtcgaccc gcgcaaggcc
    11101 gaccagatgg tgcgtggcac cgtcatcctg ccgcacggca cgggcaagac cgcgcgcgtc
    11161 atcgtgttcg ccaccggccc cgcggccgag gcggccatcg ctgccggcgc tgacgaggtc
    11221 ggcggcgccg agctcatcga caaggtggcg gccggttaca ccgcgttcga cgccgccgtc
    11281 tccacccctg agctcatggg ccaggtcggt cgtctcggta aggtgctcgg tccgcgtggc
    11341 ctcatgccga acccgaagac cggcaccgtg acgcaggaca ccggcaaggc cgtgtccgac
    11401 atcaagggcg gcaagatcga gttccgcgtc gacaagcacg ccaacgtcca cttcgtggtc
    11461 ggcaaggcgg gcttcaccga ggagcagctc agcgagaaca tcaaggccgc catcgacgag
    11521 gtcgttcgct ccaagccgag ctcctcgaag ggccgctaca tcaccaaggg caccgtgtcg
    11581 acgaccttcg gtcccggcat tccgctcgac gtgaacgcgc tctaaccaga cgcatcacac
    11641 gaaagggggt cgacttcggt cggccccctt tctgcgtacg ctggagccgt gcacattcgc
    11701 cccgcgacac cggatgacgc agccctcctg cacgagctcg ccgccgcgac cttcccgctc
    11761 gcctgcccgc ccgacacact gcccgcgtcg atcgagtcgt tcatcgccac gaacctgtcg
    11821 gtggactcgt tcacggccta cctgtccgac cccgcacggc tgctgttcgt gggcgaggtc
    11881 gacggggtcg cgagtgggta cacgatggtc gtgttcggtg aacccggtga tgcggatgtc
    11941 gcggcatccg tcaccgcgcg cccaaccgcc gagctgagta agtgctacgc cctgccctca
    12001 gcgcacggca cgggactggc cgccgccctc gtcaccgcgt cggttcaggc cgcgcgcgac
    12061 cgcggcgctc gcagcgtgtg gctgggggtc aaccagcaga acgcccgcgc gaaccgcttc
    12121 tacgagaagc agggattcgc gcaggtgggc acgaagcggt tcctggtggg ggagcgctac
    12181 gaggacgact tcgtgcgctc gctggagctt taggggagcg gtcgaccggg gcctgacggt
    12241 cgccccggcc cgacggtcga ccgggtcccg acggtcggcc agcgatatgg agcgatctac
    12301 ttcctgcttc tgttgtgcag gagtcgcg
//
LOCUS       DAOSOU010000041        12272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5157_length_12272_cov_6.932962, whole genome shotgun sequence.
ACCESSION   DAOSOU010000041 DAOSOU010000000
VERSION     DAOSOU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5157_length_12272_cov_6.932962"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLW15_03665"
     CDS             complement(<1..635)
                     /locus_tag="PLW15_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOT33531.1"
                     /translation="MSGILTMGESLGLLRAHGIGTLEHLASMDVAIGGAEGNVAIGLA
                     RLGTPATWVGVVGDDGLGQRVVRELRGQGVRVIARVNPTAPTAVMLKENPTAGLTRVT
                     YFRSAGAGRTLEPADVEALERDGELTGIQLVHVTGITPALSESAAAAVRALVDAAHRH
                     GIPVSFDVNHRSRLWTDRDPVPVYRELVASSDIAFAGEDEAALVVGGTDSTV"
     gene            complement(632..1267)
                     /gene="eda"
                     /locus_tag="PLW15_03670"
     CDS             complement(632..1267)
                     /gene="eda"
                     /locus_tag="PLW15_03670"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOT33532.1"
                     /translation="MALTATELAASAGAPVVPVIVIDDAEHAVALGEALVAGGIRTAE
                     VTFRTPAGLEAIRRMAQVGGLIVGAGTVIDPEGVERAVDAGARFIVSPGLDEAVVNRC
                     RDLDVLALPGVATASEVQHAMRLGLQAVKLFPAGVIGGAKLISALGGPFPDMQFMPSG
                     GVSLASAPEYLALASVSLVSGSWMVSRELIANQQFGEIERISRQTTEALTA"
     gene            complement(1270..3555)
                     /gene="purL"
                     /locus_tag="PLW15_03675"
     CDS             complement(1270..3555)
                     /gene="purL"
                     /locus_tag="PLW15_03675"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOT33533.1"
                     /translation="MNPVADTVQNAAATPEREQPYAALGLKPDEYEQIKQILGRRPTS
                     GELAMYSVMWSEHCSYKSSKIYLRQFGQKVSPAMKKDLMVGMGENAGVVDVGEGWAVT
                     FKIESHNHPSYIEPFQGAATGVGGIVRDIISMGARPVAVMDALRFGAIDDPDTARVVH
                     GVVSGISFYGNCLGLPNIGGETWFDPVYQANPLVNALAVGVLRHEDLHLANARGVGNK
                     VVLFGARTGGDGIGGASILASDSFSEGGPTKRPAVQVGDPFAEKVLIECCLELFAQDL
                     VEGIQDLGAAGISCATSELASNGDGGMFVELDKVLLRDPTLTAEEILMSESQERMMAI
                     VTPDKLEGFLAVTAKWDVETSVLGEVTDTGRLIINWHGEEIVNVEPRTVAVDGPVYER
                     PVAYPAWIDALQADTTARLPRSTDLKSDFLTLLGSPNLSDKSWLTNQYDRYVMGNTAL
                     SFPDDGGMVRVDEESGLGFAVATDANGRYCQLDPYRGAQLALAEAYRNVAATGARPVA
                     VSDCLNFGSPENPEVMWQFSRAVEGLADGCLELEIPVTGGNVSFYNQTGDVPIHPTPV
                     VAVLGVIDDVAARIPSGWQDEGNNIYLLGVTRDELDGSAWAGTIHGHLGGLPPAVDLA
                     AEKTLATLISAGGSESLLASAHDLADGGLGQALAEAVLRFGVGARVWISEILERDGVD
                     ATAALFSESTGRMLVSVPREDDVKFKGLCEGRGYPVLRIGVTEDSGALELQDLFTVEI
                     DELRAVHRGTLAAAFGPVVGG"
     gene            complement(3601..4152)
                     /locus_tag="PLW15_03680"
     CDS             complement(3601..4152)
                     /locus_tag="PLW15_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33534.1"
                     /translation="MTTPAARAVLAAAIIATFALAGCAPTAPATTTGDSDAPDASAGG
                     ASADCSIFAGATDPELMLFTSSAIAAGPAEGQTYGDGTELSVTLSQEAIDAGLLPQVE
                     LLTVSDTGGPVTVASLAFDPTTGGDGTYSTALKEFGNDELVGTAVVAEIFAISGATVG
                     DAKPYGDKLLLGNYCMTYANDGS"
     gene            4340..5101
                     /locus_tag="PLW15_03685"
     CDS             4340..5101
                     /locus_tag="PLW15_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT33535.1"
                     /translation="MFAFRGSSVAQTLAAYGMLVAALVAAWLLDRARRTDGLFRDLLL
                     IAVGMLIVSTVSVEADISWGNFFLLGFVLAAAVAVPYLISRFVFRDRVIRFPWRGGWP
                     WSRVQWGYIALVLGLGWLILPFYFISSGVYLNWPAVTEWHEILRLFIGVNAVGTWDEL
                     FFILTVFVLFKRHFPVWQANLLQATIFVSFLWELGYQSWGPLLTIPFALLQGWIYSKT
                     KSLPYVLTVHLLFDAIVFLVIVHARNPGALPIFLL"
     assembly_gap    5103..5202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5203..5549)
                     /locus_tag="PLW15_03690"
     CDS             complement(<5203..5549)
                     /locus_tag="PLW15_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOT33536.1"
                     /translation="MAMPPKSLFRLAAFAEAVTWTLLIAALIIRAVTGFAPVVTIAGG
                     IHGFVFLAYGATAVLLAVNQRWHPGVAAIAIVSAIVPYATIPVELWLARTGRLDGEWR
                     RETTDDPRDKVWI"
     gene            complement(5605..6306)
                     /gene="purQ"
                     /locus_tag="PLW15_03695"
     CDS             complement(5605..6306)
                     /gene="purQ"
                     /locus_tag="PLW15_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOT33537.1"
                     /translation="MRIGVITFPGSLDDHDARRAVRLGGAEAVSLWHGEHDLKGVDAI
                     VLPGGFSYGDYLRCGAIAARSPIMSEVIDAANAGMPVLGICNGFQILVESHLLPGGLV
                     RNDHGDFICRDQRLRVESTSTAWTNGFTEGQEITVPLKNGEGGFIADADTIARLEGEG
                     LVVFRYLDVNPNGSMNDIAGIANERGNVVGLMPHPEHATEPGFGPDTALAMRSGTDGL
                     TFFTSVVSSFAAQVG"
     gene            complement(6309..6587)
                     /gene="purS"
                     /locus_tag="PLW15_03700"
     CDS             complement(6309..6587)
                     /gene="purS"
                     /locus_tag="PLW15_03700"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOT33538.1"
                     /translation="MPTIVVEVMPKAELLDPAGKAVAGALHRLGKGHFTSVRIGKRFE
                     LTVDGPVDDAVLAEVRELAEEMLANTVIEDVLSVHTLDTETVTAGETH"
     gene            complement(6652..7278)
                     /locus_tag="PLW15_03705"
     CDS             complement(6652..7278)
                     /locus_tag="PLW15_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HOT33539.1"
                     /translation="MSTLRSIESLAQFVESLPTSPRRIIGIAGSPGAGKSTIAEALVA
                     RLDSAVVLPMDGYHLPQARLVELGRRDRMGAPDTFDVDGFLATLSALRAVDNSGNPVL
                     APGFDRGIEEPVPDAIRILQELSTVVVEGNYLLLESGGWGRVAPLLDATVFVRIDRGI
                     RLDRLIERHERFGKSPADARAWALGPDERNAQLIEATATRADRVVELP"
     gene            7306..8514
                     /locus_tag="PLW15_03710"
     CDS             7306..8514
                     /locus_tag="PLW15_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33540.1"
                     /translation="MSDTAVQTRTVRVLVTGQVLAGLGQGATLAIGAVIAAEMAGEAF
                     AGAAAVASTLGAAAASIPLARFALRLGRRPALALGAVIAALGSVLTVVATGLSAFPLL
                     LVAFALLGVGTSVGLQARFAATDVASPERRGRDLSLVVWSTTIGAVVGPNLWGPGTVI
                     QNALGLPEHTGSFVIAIVAQLAAAGLYLVALRPDPLVLARGREVALAPAADAGIVRGR
                     SALVFAIGTIALSHAVMVSIMAMTPVHMTSHGASLTIVGFTISLHIAGMYALSPLFGW
                     ASDRFGRLTVILAGQGMFAVSVLLIGTSPENTAAVTVALVLLGLGWSASTVSGSALVS
                     DLVTGEARVRVQGRTDTIMSLAGAVGGASAGPVLALLGYAGVAWAAGALVLVILLASG
                     VMGRRVATVR"
     gene            8545..9645
                     /locus_tag="PLW15_03715"
     CDS             8545..9645
                     /locus_tag="PLW15_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03364 family FAD-dependent oxidoreductase"
                     /protein_id="HOT33541.1"
                     /translation="MPDDDSFDLAVVGAGIVGLAHAEAAAQRGLRVVVVERSTQITGS
                     SVRNFGHVGVGMHVGDARHYADLSRGIWMRLAEAAGFWLRESGSLMVARADDELAVLE
                     ESGVGELLTARQVADAAPVVGALGGMRSPLDLQVDPRAAAPAIARHLAERGVEFRWRT
                     TALGAETGVLHTSRGPIRAQAIVVAVNFDVDQLYPELAERYGVVRCALDMLLADGVGL
                     GLPLLTGSTMLRYSAFASAPSAAAVRARFEREHPELLDLDINQMYTERPDGTLVIGDT
                     HVRDAAVSPFQSEASFSVLERLTVDLFGRPISVRERWQGVYASAPAEFLIEAPADGVR
                     VVAVTTGIGMTTGLGLGESVITDLFSPTRGAP"
     gene            9642..10307
                     /locus_tag="PLW15_03720"
     CDS             9642..10307
                     /locus_tag="PLW15_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonatase-like hydrolase"
                     /protein_id="HOT33542.1"
                     /translation="MTTPFELVVFDMAGTTVVDDGLVEAAFGRAWDAVVATAEGRDAA
                     MEHVRATMGQSKIDVFRGLGDEETARAMNAAFEGAFRALIEEGRCEPIPGAEDAIRSL
                     KSKGLAIAFTTGFSHDTADLIIESLGWVGLADVVLTPDDAGRGRPAPDLPLTALLRTG
                     TSSVAAMVVVGDTESDAASGRAAGAGLVVGVLTGGRDEERLREAGADVVLASVAELPA
                     ALA"
     gene            complement(10299..11054)
                     /locus_tag="PLW15_03725"
     CDS             complement(10299..11054)
                     /locus_tag="PLW15_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT33543.1"
                     /translation="MSTLHRLSRVAMIPVAPVLVAQGRRLRRDTPRLPDAASPWHGTV
                     AGPRPLSLLVLGDSTAAGVGADTQDDALPGHLGRELEARTGRGVQWRAIGENGADARE
                     LIERYLDDATSAPYDVVFLSVGANDALGIRSRGAFVRDIRQLLSRLRAASPDAVILVS
                     SLPAFFRFELLPQPLKWNLYLHSTSLENGARAVVATMADVHMSPPPPPYTDGFFASDL
                     FHPSAAGYRDWARFAIEDAASAGALRRLMATSG"
     gene            11122..12132
                     /locus_tag="PLW15_03730"
     CDS             11122..12132
                     /locus_tag="PLW15_03730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT33544.1"
                     /translation="METSGLDEFLAEQQFSGVVLVRRGPTTVYEAATGLATPRWGIPN
                     TLDTRFDTASITKLFTSAAVLHLVGRGELDLETSIHHYVDLEGTTISPDVTLLHLLTH
                     TSGIADDADEEAGEKYEDLFLEKPNYAIIETADFLPQFAHKEPLAAPGVECRYCNVGY
                     VLAGLAIERVTGQSYRQYVFDEVFIRAGMLDSGFYDRREAAPRVAEGWDLIDGVWRSN
                     IYSYPPIGSPDGGAHVTASDLVRFIQAVRGGELLNAEFTTEFLLPQVEHDEATWYGFG
                     LEFDMNEDGTVRSYYKDGINPGASGIVRHYLEEGLDIVVLSNTEEGAWPVIQELDERL
                     GG"
BASE COUNT         1734 a         4165 c         4317 g         1956 t
ORIGIN      
        1 acggtgctgt cggtgccacc caccacgagc gcggcctcgt cctcaccagc gaacgcgatg
       61 tccgagctcg ccacgagctc gcggtacacg ggcaccgggt cgcggtcggt ccagagtcgc
      121 gagcggtggt tcacgtcgaa cgagaccggg atgccgtgtc tgtgcgccgc gtcgacgagg
      181 gcgcgcaccg cggccgccgc cgactccgac agggcggggg tgattccggt gacgtgcacg
      241 agttggatgc ccgtcagttc gccatcgcgc tccagcgcct cgacatcggc gggctccagg
      301 gtgcgccccg cgcccgccga gcggaagtag gtgacccgcg tcaggcccgc cgtggggttc
      361 tccttgagca tgacggcggt cggcgcggtg ggattcacgc gggcgatcac tcgaactccc
      421 tgcccgcgca gttcccgaac gaccctctgg cctagcccgt cgtcgccgac gacacccacc
      481 caggtggccg gtgtgccgag tcgggcgagg ccgatggcga cgttgccctc ggcgccaccg
      541 atcgcgacat ccatcgacgc gaggtgctcg agcgtgccga tgccgtgcgc ccgcaggagc
      601 ccgaggcttt cgcccatggt gaggatgccg ctcatgctgt cagcgcttcc gtggtctgcc
      661 tcgagatccg ttcgatctcc ccgaactgct ggttcgcgat cagctcgcgc gacaccatcc
      721 agctgccgct gaccagagac accgacgcca aagccaggta ctccggcgcc gaggcgagtg
      781 acaccccgcc cgagggcatg aactgcatgt cggggaaggg tccgccgagc gcgctgatga
      841 gcttcgcacc cccgatcacc cccgcgggga agagcttcac cgcctgaagt cccagccgca
      901 tcgcgtgctg cacctcgctg gccgttgcga caccgggcag ggcgagcacg tccaggtcgc
      961 ggcagcggtt cacgaccgcc tcgtcgagcc caggcgacac gatgaagcgc gcgccagcat
     1021 ccaccgctcg ctcgacgccc tcgggatcga tcacggtgcc ggcgccgacg atgaggccgc
     1081 cgacctgtgc cattcggcgg atcgcctcga ggccggccgg ggtgcggaac gtgacctcgg
     1141 cggtacggat gccgccggcg acgagcgcct cgccgagcgc aaccgcatgc tcggcgtcat
     1201 cgatcacgat cacgggcacg accggcgcgc cagcagaggc ggcgagttcg gtcgcggtga
     1261 gtgccacgct cagccgccga caaccggtcc gaatgcggcg gcgagggttc cccggtgcac
     1321 cgcgcgcagc tcgtcgatct cgacggtgaa caggtcctgc agctcgaggg cgcccgagtc
     1381 ctcggtgacg ccgatgcgca gcaccgggta gccgcgaccc tcgcacaggc ccttgaactt
     1441 cacgtcgtcc tcgcgcggaa cgctcaccag catcctgccg gtcgactcgc tgaacagggc
     1501 cgccgtcgca tcgacaccgt cgcgctcgag gatctcgctg atccacaccc gcgcgccgac
     1561 accgaaccgc agcaccgcct ccgcgagtgc ctgccccagc ccgccgtcgg ccaggtcgtg
     1621 agcggatgcc agcaggctct cgctcccgcc ggccgagatc agcgtcgcaa gtgtcttctc
     1681 cgcggcgagg tcgaccgcgg gtggcaggcc gcccaggtgc ccgtggatcg ttcccgccca
     1741 cgccgagccg tcgagctcgt cgcgcgtgac gccgagcagg tagatgttgt tgccctcgtc
     1801 ctgccagccg ctcgggatgc gcgcggccac gtcgtcgatc acgccgagca cggcgaccac
     1861 cggggtcggg tggatcggca catcgccggt ctggttgtag aagctgacgt tgccgccggt
     1921 cacggggatc tcgagctcga ggcatccgtc ggccaggccc tcgacggcgc gggagaactg
     1981 ccacatgacc tcggggttct ccggggagcc gaagttgagg cagtcgctga ccgccacggg
     2041 gcgcgcgccg gtggcggcga cgttgcggta cgcctcggcg agcgcgagct gcgcgccgcg
     2101 gtaggggtcg agctggcagt agcggccgtt cgcatcggtc gcgacagcga agccgagccc
     2161 gctctcctcg tcgacgcgca ccatgccgcc gtcgtcgggg aagctcaggg cggtgttgcc
     2221 catcacgtag cggtcgtact ggttggtcag ccacgacttg tcgctgaggt tgggcgagcc
     2281 cagcagcgtg aggaagtcgg acttgaggtc ggtcgaacgc ggcaggcgcg cggtcgtgtc
     2341 ggcctgcagc gcgtcgatcc aggcggggta ggcgaccggg cgctcgtaga ccggaccatc
     2401 gacggcgacg gtgcgcggct cgacgttgac gatctcctcg ccgtgccagt tgatgatgag
     2461 gcgaccggta tcggtgacct cgccgagcac gctcgtctcg acatcccact tggcggtcac
     2521 cgcgaggaag ccctcgagct tgtccggcgt gacgatcgcc atcatgcgct cctggctctc
     2581 cgacatgagg atctcctcgg ccgtgagagt ggggtcgcgc agcagcacct tgtcgagctc
     2641 gacgaacatg ccgccgtcac cgttgctggc cagctcgctc gtcgcgcagc tgatgcccgc
     2701 tgcgcccagg tcctggatgc cctccacgag gtcctgcgcg aacagctcga ggcagcactc
     2761 gatgagcacc ttctcagcga acgggtcgcc cacctgcacg gcgggacgct tggtcggccc
     2821 gccctctgag aacgagtccg aggcgagaat ggatgccccg ccgatgccat ccccgccggt
     2881 gcgcgccccg aacagcacga ccttgttgcc cacgccccga gcattggcca ggtgcaggtc
     2941 ctcgtggcgc agcacgccga cggccagagc gttcacgaga ggattggcct ggtacaccgg
     3001 gtcgaaccag gtctcgccgc cgatgttggg caagccgaga cagttgccgt agaacgagat
     3061 accggagacc acgccgtgca cgacgcgggc cgtgtcgggg tcgtcgatcg cgccgaagcg
     3121 caacgcatcc atgacggcga ccgggcgagc ccccatggag atgatgtcgc gcacgatgcc
     3181 gcccacgccg gtggcggcgc cctggaacgg ctcgatgtag ctcgggtggt tgtggctctc
     3241 gatcttgaag gtgaccgccc agccctcacc gacatccacg acgccggcgt tctcgcccat
     3301 gcccaccatg aggtccttct tcatggcggg gctgaccttc tggccgaact gccgcaggta
     3361 gatcttggag ctcttgtagg agcagtgctc gctccacatc accgagtaca tggcgagctc
     3421 gccgctcgtg gggcggcgcc cgaggatctg cttgatctgc tcgtactcgt cgggcttcag
     3481 gccgagggca gcgtagggct gctcgcgctc cggggtcgct gcggcgttct ggacagtgtc
     3541 agcaaccgga ttcacggccc aattctatcc agcgccgctc gccgcattcg cggcacccgg
     3601 ctaggagccg tcgttcgcgt acgtcatgca gtagttgccg agcagcagct tgtctccgta
     3661 gggcttggcg tcgcccacgg tcgcaccgct gatcgcgaag atctcagcga cgacggctgt
     3721 gcccacgagc tcatcgttgc cgaactcctt caaggcggtc gagtaggtgc catcgccacc
     3781 ggtcgtcggg tcgaaggcca ggctcgccac cgtaacgggg ccgccggtgt cgctgacggt
     3841 caacagctcg acctgcggca gcagccctgc gtcaatcgcc tcctgcgaga gagtgaccga
     3901 cagctcggtg ccgtcaccgt aggtctggcc ctcggcggga ccagcagcga tcgctgacga
     3961 ggtgaacagc atgagctcgg gatcggtcgc cccggcgaag atgctgcagt cggcgctcgc
     4021 gccgcccgca gaggcgtcgg gcgcgtcgct gtcacccgtg gtcgtagcgg gggcggtggg
     4081 cgcgcagcca gcgagggcga aggtcgcgat gatggcggcg gcaagaacgg cacgtgcggc
     4141 tggagtcgtc atgaaaccag tcttaccgag aacggtgcga gaggaagtgg ggattgtacg
     4201 catcttcatc gggctgcggg tgcggttgcg gctgtgcgaa gctggtgccg tgcccgccgc
     4261 cgacacgctc gacaccccgc gccccgcctg gagctgggct ctgctgccgg gcatcctgct
     4321 ggcggggtcg gcgccgctca tgttcgcgtt ccgcggcagt tcggtcgccc agaccctggc
     4381 cgcctacggg atgctcgtgg ccgccctcgt cgccgcctgg ctgcttgacc gcgctcgccg
     4441 caccgacgga ctcttccgcg acctgctgct catcgcggtg ggcatgctca tcgtcagcac
     4501 ggtctccgtc gaggcggaca tctcgtgggg caacttcttc cttctgggct tcgtgctcgc
     4561 ggccgctgtc gcggtgccgt acctgatctc gcggttcgtg ttccgcgacc gcgtcatccg
     4621 gttcccgtgg cgcggcgggt ggccgtggtc gcgcgtgcag tgggggtaca tcgcactcgt
     4681 gctggggctc ggatggctca tcctgccctt ctacttcatc tcctcgggtg tctacctcaa
     4741 ctggccggca gtcaccgagt ggcacgagat actgcgactg ttcatcggcg tcaacgcggt
     4801 cggcacgtgg gacgagctgt tcttcatcct cacggtgttc gtgctgttca aacggcactt
     4861 cccggtgtgg caggcgaacc tgctgcaggc gacgatcttc gtctcgttcc tgtgggagct
     4921 cggctaccag tcctggggtc cgctgctgac gatcccgttc gcgttgctcc agggctggat
     4981 ctactccaag acgaagtcac tgccctacgt gctgaccgtg cacctgctgt tcgatgcgat
     5041 cgtgttcctc gtgatcgtgc acgcgcgcaa cccgggggcg ctgccgatct tcctgttgtg
     5101 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgatcca gaccttgtcg
     5221 cgcgggtcat ccgtcgtctc gcgacgccac tcgccgtcga gacgcccggt gcgggcgagc
     5281 cagagttcga ccgggatggt cgcgtagggc acgatcgcgc tcacgatcgc gatggcggcg
     5341 acacccgggt gccagcgctg gttcacggcg agcagcaccg cggtggcgcc gtaggcgagg
     5401 aagacgaaac cgtggatgcc tcccgcgatc gtcactacgg gcgcgaaccc ggtcaccgcg
     5461 cgaatgatga gcgcggcgat caggagagtc caggtgacgg cttcggcgaa ggcggcgaga
     5521 cggaagaggg acttgggggg catggcgatc ctgcgggtcg gggtgggcgg ggctgttggt
     5581 ttcgatacgc ggcttcgccg ctactcaacc aacctgggcg gcgaacgacg acacgacgct
     5641 cgtgaagaag gtgagtccgt cggtgcccga gcgcatggcc agggcagtgt cggggccgaa
     5701 gccgggctcg gtggcgtgct ccgggtgggg catgaggccc acgacgttgc cgcgctcgtt
     5761 ggcgatgccc gcgatgtcgt tcatcgaccc gttggggttc acgtcgaggt agcggaacac
     5821 gacgagaccc tcgccctcga gccgggcgat cgtgtcggcg tctgcgatga agccgccctc
     5881 gccgttcttc agcggaacgg tgatctcctg gccttcggtg aacccgttgg tccacgcggt
     5941 cgaggtgctc tccacgcgca ggcgctggtc ccggcagatg aagtcgccgt ggtcgttgcg
     6001 caccagaccg ccgggtagca ggtgggattc cacgagaatc tggaagccgt tgcagatgcc
     6061 gagcacgggc atcccggcat tggcggcgtc gatgacctca ctcatgatcg gcgaacgcgc
     6121 ggcgatcgcg ccgcagcgca ggtagtcgcc gtagctgaag ccgccgggca gcacgatcgc
     6181 gtcgacgccc ttgaggtcgt gctcgccgtg ccagaggctc acagcctccg cgccgccgag
     6241 gcgcaccgcg cgacgggcat cgtggtcgtc gagggagccg gggaaggtga tgacgccgat
     6301 gcgcatgctc agtgcgtctc ccctgcggtg accgtctcgg tgtcgagggt gtgcacgctg
     6361 agaacgtctt cgatcaccgt gttggcgagc atctcctcgg ccaactcgcg cacctcggcg
     6421 agcaccgcgt catccaccgg accatcgacg gtgagttcga agcgcttgcc gatgcgcacc
     6481 gaagtgaagt gacccttgcc gagccggtgg agtgccccgg cgacggcctt gcctgctggg
     6541 tcgagaagtt cagccttcgg catgacctcg acgacgatgg tgggcatgcg tggctcccgc
     6601 tcgtgagtcg gtggtggatt tgtgcaccag tctaccggcg gcgcgtcggg ctcacgggag
     6661 ctcgaccacg cggtctgcgc gggtggcggt cgcctcgatg agctgcgcat tgcgctcgtc
     6721 ggggccgagg gcccaggccc gggcatccgc ggggctcttg ccgaagcgct cgtggcgctc
     6781 gatcaggcgg tcgaggcgga tgccgcggtc gatccgcacg aagacagtgg cgtccagcag
     6841 cggcgcaacg cgcccccacc cgccggactc gagcagcagg tagttgccct ccaccaccac
     6901 tgtcgataac tcctgcaaaa ttctgatggc atcgggcacg ggctcctcga ttccgcggtc
     6961 gaacccgggc gcgagcaccg gattgccgga gttatcgaca gcgcggaggg cggacagggt
     7021 cgcgaggaac ccgtcgacgt cgaacgtgtc gggcgcgccc atgcggtcgc ggcggcccag
     7081 ttcgacgaga cgcgcctgcg gcaggtggta cccatccatc ggcagcacga ctgcggagtc
     7141 cagccgggcc accagcgcct cggcgatcgt cgacttgccg gcgccggggc tgccggcgat
     7201 accgatgatg cgtcggggcg aggttggcag ggattcgacg aactgcgcca acgactcgat
     7261 cgaacgcagc gtgctcacgc cctcacgcta gagcagactg ggcgggtgag tgataccgca
     7321 gtgcagaccc gaaccgtgcg cgtgctcgtc accggtcagg tgcttgcggg actcgggcag
     7381 ggtgcgaccc tcgccatcgg tgccgtgatc gccgccgaga tggcggggga ggcgttcgcg
     7441 ggtgccgcgg cggtggccag cactctcggt gcggcagcgg catccatacc gctcgctcgc
     7501 ttcgcgcttc ggctcgggcg tcggcccgct ctcgcgctcg gtgcggtgat agccgcgctc
     7561 ggctcggtgc tcaccgtggt cgcgaccgga ctctccgcct tcccgctgct gctcgtcgcg
     7621 ttcgctctgc tgggggtcgg cacgtcggtg ggtctgcagg cacgcttcgc ggccacggat
     7681 gtcgcctccc ccgagcggcg cggacgtgac ctgtcgctcg tggtgtggtc gaccaccatc
     7741 ggtgcggtgg tcggacccaa cctgtgggga ccgggcaccg tcatccagaa cgccctcggc
     7801 ctgcccgagc acaccgggtc gttcgtcatc gcgatcgtcg cgcagctcgc cgcggccggt
     7861 ctctacctcg tggccctgcg ccccgacccg ctcgtgctgg cccgcggtcg tgaggtagca
     7921 ctggccccgg cggcggatgc tggcatcgtg cgcggtcggt cggcgctggt cttcgcgatc
     7981 ggcacgatcg ctctcagcca cgcggtcatg gtctcgatca tggcgatgac ccccgtgcac
     8041 atgacgagcc acggcgcgag cctgacgatc gtcggcttca cgatcagcct gcacatcgcg
     8101 ggcatgtacg cgctgtcgcc gctgttcggg tgggccagcg accggttcgg cagactcacg
     8161 gtgatcctgg cgggtcaggg gatgttcgct gtctcggtgc tgctgatcgg cacctccccg
     8221 gagaacacgg ccgccgtgac cgtcgcgctc gtgctgttgg gtctggggtg gagcgcctcg
     8281 acggtgtccg gctccgcgct ggtctccgac ctcgtcacgg gtgaggcgcg cgtgcgggtg
     8341 cagggtcgca ccgacacgat catgagtctt gcgggcgcgg tcggtggcgc cagtgccgga
     8401 ccggtgctgg ctctcctcgg ctacgccggt gtcgcgtggg cggcgggtgc gctggtgctg
     8461 gtgatcctgc tcgcctctgg tgtgatgggc cgacgcgtgg cgacggtgcg ctagtgactg
     8521 cgccgtgctg cgaggggagc ttcgatgcct gacgacgaca gcttcgatct cgcggtcgtg
     8581 ggcgcgggaa tcgtcgggct cgcccacgcg gaggccgcgg cgcagcgagg cctgcgcgtc
     8641 gtggtggtcg agcgatcaac ccagatcacc gggtcgagcg tgcgcaactt cggccacgta
     8701 ggcgtcggga tgcatgtcgg ggatgcccgc cactacgccg acctctcccg gggcatctgg
     8761 atgcgcctgg ccgaggccgc gggattctgg ctgcgcgaga gcggctcgct catggtcgct
     8821 cgggccgacg acgagctggc cgtgctcgag gagtcgggcg tcggcgagct gctcacggcc
     8881 cggcaggtgg ccgacgccgc gcctgtcgtg ggcgcgctcg gcggcatgcg cagccccctc
     8941 gatctgcagg tcgacccgcg cgcggccgca ccggccatcg cgcggcatct cgccgagcgg
     9001 ggcgtcgagt tccgctggcg cacgacggcg ctgggggcgg agaccggggt gctccacacc
     9061 agtcgcgggc cgatccgtgc gcaggcgatt gttgtcgcgg tcaacttcga tgtcgaccag
     9121 ctctatccgg aactcgccga acgctacggc gtcgtgcgct gcgcactcga catgcttctc
     9181 gccgacggag tcgggctggg gctgcccctg ctgaccgggt cgaccatgct gcgctactcg
     9241 gcgttcgcgt cggcaccctc ggcggcggcg gttcgggcga ggttcgagcg cgagcatccc
     9301 gaactgcttg acctggacat caaccagatg tacaccgagc gtcccgacgg aacgctcgtg
     9361 atcggagaca cccacgtgcg cgacgccgcg gtgtcgccgt tccagtccga ggcgtcgttc
     9421 tcggtgctgg agcggttgac cgtcgacctc ttcggccgcc cgatctccgt gcgcgaacga
     9481 tggcaggggg tctacgcgtc ggcgccggcc gagttcctga tcgaggcacc ggccgacggg
     9541 gtgcgggtcg tcgccgtgac cacgggcatc ggcatgacga ctggcctggg gctgggcgaa
     9601 tccgtgatca ccgacctgtt ctcaccaacc agaggagcac catgaccacc ccgttcgaac
     9661 tcgtcgtgtt cgacatggcc ggcacgacgg tggtcgacga cggcctcgtc gaggcagcct
     9721 tcggccgcgc gtgggacgcc gtcgtcgcca ccgcggaggg gcgcgatgcc gccatggagc
     9781 acgttcgcgc gaccatgggg cagtccaaga tcgacgtctt ccgcgggctg ggtgacgagg
     9841 agacggcccg ggccatgaac gcggcgttcg agggcgcttt ccgcgcgctg atcgaggagg
     9901 gtcgctgcga gccgatcccg ggggcggagg atgccatccg ctctctcaag tccaaggggc
     9961 tggcgatcgc cttcacgacc gggttctcac acgacaccgc tgacttgatc atcgagtcgc
    10021 tcggctgggt ggggctcgcg gatgtcgtgc tcacccccga cgacgccggt cgcggtcggc
    10081 ccgctcccga ccttccgctc acggcgctgc tgcgcaccgg taccagctcg gtggccgcca
    10141 tggtggtcgt gggcgacacc gagtcggatg cggcgagcgg ccgggcggcc ggtgccggtc
    10201 tggtggttgg ggtcctcacc ggcgggcgtg acgaagagcg attgcgcgag gccggcgccg
    10261 acgtcgtgct cgcgagcgtc gctgagttgc ctgcggccct agcctgacgt agccatcagg
    10321 cgacgcaggg ccccggctga agcggcatcc tcgatcgcga agcgcgccca gtcccgatag
    10381 ccggcagcgc tcgggtggaa caggtcgctg gcgaagaagc cgtcggtgta cggcggcggg
    10441 ggcggcgaca tgtgcacgtc ggccatggtg gcgacgaccg cgcgggcacc attctccagg
    10501 ctcgtggagt gcaggtagag gttccatttg agcggctgcg gcagcagctc gaaccggaag
    10561 aacgcgggca gcgagctcac cagaatcacc gcgtccgggc tcgcggcgcg cagccggctc
    10621 agcagctggc ggatgtcgcg aacaaacgca ccccgcgacc ggatgccgag cgcgtcgttg
    10681 gcccccacac tcaggaacac cacgtcgtag ggcgcgcttg tcgcgtcatc caggtagcgc
    10741 tcgatgagtt cccgggcgtc ggcgccgttc tccccgatag cgcgccactg aacaccgcgg
    10801 ccggtgcggg cctcgagctc gcggccgagg tggccgggca gcgcgtcatc ctgggtgtcg
    10861 gcgccgacgc ccgcggcagt cgaatccccc agcacgagga ggctcagcgg ccggggaccg
    10921 gcgacggtgc cgtgccaggg ggaggcggca tccggcagtc gaggggtgtc gcgtctgagt
    10981 cggcgaccct gcgcgaccag cacgggcgcg accgggatca tggccacgcg gctgaggcgg
    11041 tgcagggtgc tcacgcgccc atcgtagggc gaagccgtcg cctctagcgc ccgccgccga
    11101 cccgatcggt aggctcgggg gatggagact tccggactcg acgagttcct ggccgagcag
    11161 cagttctcag gggtcgtgct cgtgcgccgc ggtccgacga ccgtgtacga ggcggcgacc
    11221 ggcctcgcga cgccgcggtg gggcatcccg aacaccctgg acacccgctt cgacaccgcc
    11281 tccatcacca agctcttcac gagcgcggcg gtgctgcacc tcgttggcag gggcgaactc
    11341 gacctcgaga cctcgatcca ccactacgtc gacctcgagg gcacgacgat cagccccgac
    11401 gtcacgctgc tgcatctgct cacgcacacg agcggcatcg ccgatgacgc cgacgaggag
    11461 gcgggcgaga agtacgagga cctgttcctc gagaagccca actacgcgat catcgagacg
    11521 gccgacttcc tgccccagtt cgcccacaag gagccgctcg ccgcgcccgg cgtcgagtgc
    11581 cggtactgca acgtcggcta cgtgctcgcc gggctcgcca tcgagcgggt gaccggccag
    11641 tcgtatcggc agtacgtgtt cgacgaggtc ttcattcggg ccgggatgct cgactccggc
    11701 ttctacgacc gtcgagaagc ggctccgcgc gtcgcggagg ggtgggacct catcgacggc
    11761 gtctggcgct ccaacatcta cagctacccg ccgatcggat ccccggacgg tggcgcgcac
    11821 gtcaccgcat ccgacctcgt gcgcttcatc caggcggtgc gtggcggtga gctgctcaac
    11881 gcggagttca cgaccgagtt cctgctgccc caggtcgagc acgacgaggc gacctggtac
    11941 ggcttcggcc tcgagttcga catgaacgaa gacggcaccg tgcgttcgta ctacaaggac
    12001 ggcatcaacc cgggggcctc cgggatagtg cgccactacc tcgaggaggg cctcgacatc
    12061 gtcgtgctct ccaacaccga ggagggcgcg tggccggtca tccaggagct cgacgagagg
    12121 ctcggcggct gagatgacca cgccgcgggt cgtgctggcg ctgctggtcg gtgccgcgct
    12181 cgtggccctc tccggatgcg ccgccccggc acccgacacc acccccatcg atctccgcgc
    12241 cagcccgacc gccgcacgcg accggctgct cc
//
LOCUS       DAOSOU010000042        12233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5184_length_12233_cov_5.301938, whole genome shotgun sequence.
ACCESSION   DAOSOU010000042 DAOSOU010000000
VERSION     DAOSOU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5184_length_12233_cov_5.301938"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PLW15_03735"
     CDS             complement(<1..1193)
                     /locus_tag="PLW15_03735"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HOT33545.1"
                     /translation="MTNTIRTQVGILGAGPAGLLLAHLLGKAGIDSVVIDARSREDIE
                     GTVKAGIVESPAAEVLAESGVSDRIYREGMKHDGVEFHFDGRAHRFDFQELVGKSVYL
                     YPQHEVLKDLIAARIRDGVTPLFEHTATSVEDAESDKPRIVGTTAAGEPFEIVADVVV
                     GADGSGSIARIPVVGSQQGGYYREYPFAWYGVLVEAPPSAPELIYSRSDEGFVLISTR
                     DQHIQRMYFQCDPLLDPTTVSDAEIWDKLQAGIEGTTLKQGPIFRKDVLRFRSFVAHE
                     LRKGRVFLMGDAAHTVPPTGAKGMNLAFSDVLWLNQALRSFFATGSEELMAAYPENAL
                     KRIWKAQHFSYWMTTMLHITPDATEFDKRRGLGELALLTDSLHAQRYLAESYTGWDYG
                     VDDWR"
     gene            1228..>1619
                     /locus_tag="PLW15_03740"
     CDS             1228..>1619
                     /locus_tag="PLW15_03740"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT33546.1"
                     /translation="MRNFHEMEVLDRAMLVLGCFGPEANALTLTQISERTGLALSTCH
                     RIVASLVAGGFLDRGDDRRYRVGTTLWTIAQHGPLSDRLRESALPALARLYEETGENI
                     TLAVLDNGQALYVERLMGERSIPTVSRAG"
     assembly_gap    1620..1719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1720..1972
                     /locus_tag="PLW15_03745"
     CDS             <1720..1972
                     /locus_tag="PLW15_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOT33547.1"
                     /translation="RDSIDQPDRLRRDLAEVRQRGYSITRQEMTVGSGSIAVPILVDG
                     QCVAAVGVIVHLARLDVNRLVGTLNRAAATIVTELGRLK"
     gene            1993..2787
                     /locus_tag="PLW15_03750"
     CDS             1993..2787
                     /locus_tag="PLW15_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT33548.1"
                     /translation="MSEKGAAAQDAPGPSVTGRALAVLDSFDQTHRRQSLAAISRRTG
                     LPLTTVHRLVHELERHGALARGADGDYEIGSKIWRLGTLASVHVELREVALPYMEDVY
                     QLANDAVQIAVLDGLRILVVDRIAGSRSIEVLSKPGSRLPLHATGVGKVLLAYGGQDL
                     QDAVLGSLDRYTDSTITDAPTLKAQLLTIRSQGFAVTREELAPGATSVAVPLRGKGGT
                     VIAALGVVSSSDNAEISRMVPVLQVTAAALSKKLAQSGLGEASRLR"
     gene            2882..3577
                     /locus_tag="PLW15_03755"
     CDS             2882..3577
                     /locus_tag="PLW15_03755"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HOT33549.1"
                     /translation="MARVVRGTPRTPLEIVDGLAELGVATVHEAQGRIGLLNVRLRPI
                     FPAKIAGTALTAEVAPGDNWSIHVAVEQAQPGDILVVTPTSPCEDGYLGDLLADSLLA
                     HGVRGLVIDAGVRDVATLTEMGFPVWSKNIGAQGTVKFTPGNVQTPIVIAEQLVNPGD
                     VIVADIDGVVVVRREDAEAVLAKGREREANETAKRERLRAGELGLDIYGMRDKLAAAG
                     LVYVDYRPEDYAG"
     gene            3574..4677
                     /locus_tag="PLW15_03760"
     CDS             3574..4677
                     /locus_tag="PLW15_03760"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HOT33550.1"
                     /translation="MTPTNSQRSVPGALMRGGTSKGLYFLADDLPHDRAARDEFLLAA
                     MGSPDVREIDGLGGGHPLTSKVAVVSRSDREGIDVDYLFLQVWPDRAEVSDQQNCGNI
                     LAGVGPFAIERGLVAPSGDLTPVAIFMVNTDSKATAFVETPGGVVNYAGTARIDGVPG
                     THAPIMINFEDVAGSSCGALLPTGNAVDTVLGVDVTCIDNGMPVVCLAASDFGITGYE
                     TPAELEANEELKARVEAIRLAVGPMMNLGDVTNKTVPKMSLLAPARDGGLVATRTFIP
                     HRVHESIGVLGAVSVATACMIDGSVAARIAGLSTAATDLVVEVEHPTGFFTVQMEVDR
                     SSGELVVTKSALLRTARMLMSGDIYVIDSGEAA"
     gene            4674..5693
                     /locus_tag="PLW15_03765"
     CDS             4674..5693
                     /locus_tag="PLW15_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT33551.1"
                     /translation="MIIDIHGHYTTAPEAHNQWRIDQLAAYAAGQPSPAYPEISDDTI
                     RASIEENQLKLITERGSDLTIFSPRASAMGHHEGDQRISLEWTVACNNLIKRVVDLFP
                     DTFVGVGQLPQSPGSDLTATIAELERVVNELGFIGVNLNPDPSGGLWQTVPLTDRFWY
                     PLYEKMVELDVPAMVHVSASCNPNFHATGAHYMNADTTAFMQLIQGDLFADFPTLRLI
                     IPHGGGAVPYHWGRYRGLADMLKQPDLSTHVMNNVFFDTCVYHQPGIDLLVKVIDNKN
                     ILFGSEMIGAVRGIDPTTGQYFDDTKRYIDAAQISDAQKASIFESNARAVFPRLDAIL
                     AERGR"
     gene            5690..6583
                     /locus_tag="PLW15_03770"
     CDS             5690..6583
                     /locus_tag="PLW15_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT33552.1"
                     /translation="MSAAPAFVKDADWLDWDPDPTKPAFVVPAGSVDAHCHVFGPGDI
                     FPFAPERKYTPCDASREQLFALRDFLGFDKNVVVQATCHGADNSALLDVLEHSEDRAR
                     GVVTVKPDIEARELARMDDLGVRGVRFNYVKRLVDPKPDDYYRAIVDKIRPLGWHVVL
                     YFEPADLAEKRDFFASLGVPIVVDHMGRPDVTKPVDGPEFAAYLDFARTADVWTKVSC
                     PDRLTVSGSPHYVDVVPFARAIVEEFSDRVLWGTDWPHPNMKKEMPDDGILVDYVPSI
                     ATTVALQEKLLVTNPTTLYWS"
     gene            6586..6987
                     /gene="ligA"
                     /locus_tag="PLW15_03775"
     CDS             6586..6987
                     /gene="ligA"
                     /locus_tag="PLW15_03775"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: protein motif:HMM:NF009917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HOT33553.1"
                     /translation="MTKPDFHQFDDIPGTTIFTIEKAREGFALNQFCMSLRKAENRER
                     FLADNRAYLDEWDMTEEQKQAVIDRDFQAMLDHGGNVYFLSKIFASEGLSYVQAVSTM
                     TDMNVEQYQAMMLAGGRNVEGWRSKKERGEA"
     gene            6987..7832
                     /locus_tag="PLW15_03780"
     CDS             6987..7832
                     /locus_tag="PLW15_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HOT33554.1"
                     /translation="MARITAGVSTSHVPAVGAAVDMGRTEEPYWKPMFEGYEFSKKWM
                     ADHTPDVAIIVYNDHASYFGMEIMPTFAMGIADSFAICDEGYGPRPVPVVEGAPELAT
                     HLAASLILDDFDMTVLNVLEVDHGLTVPMSLMFGTPDKWPTKVIPLPVNVVMYPQPSG
                     ERCLSLGKAIRRAVQQFDEDLDVQIWGTGGMSHQIQGARAGLINLPWDIQFMDDLINQ
                     PEVLQYKPHLEYMIEAGSEGVELIMWLIMRGALGDQVDEVYRFNHVPASNTSVGHLIL
                     TPKEI"
     gene            7835..8785
                     /locus_tag="PLW15_03785"
     CDS             7835..8785
                     /locus_tag="PLW15_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT33555.1"
                     /translation="MTVNLAVVGLGAFGQKHLDALAQIPDAVIHSVAHSKMDVAQEVA
                     AKYGAAHAFTDYSALLAQPDLDGVLLATPTQMHHAQTIEALRAGKHVMVEIPMADSLA
                     GVKEIVDVQAETGRIAMAGHTRRFNPSHQYLHKKIVAGEFAIQQMDVQTYFFRRTNMN
                     ALGQPRSWTDHLLWHHAAHTVDLFQYQTGERIVKANAIQGPKHPELGIAMDMSIQLKT
                     ETGKICTLSLSFNNDGPLGTFFRYIGDTGTYIARYDDLVTGKEEPIDVSHVDVSMNGI
                     ELQDREFVAAIVEGREPNSSVAQVLPCYVVLDELQRQLEY"
     gene            complement(8866..9660)
                     /locus_tag="PLW15_03790"
     CDS             complement(8866..9660)
                     /locus_tag="PLW15_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HOT33556.1"
                     /translation="MSSRVRIGVIGLGEAGSLIAAGLQAAGADVIGFDPVVPETPPVP
                     LAPDVAAVVTGADIVISINSSTAAKKVAEQVAPLLGAGTVYADLNTGTPALKKALAGL
                     FGDGVFADVAIMKPVPGLAEKVPMGVAGPGARRFVDLLEPYGMNLEYVSDVPGEAAAR
                     KLLRSILAKGMAAVLIDTLWAAESTGLQQWAYEEILREFDSSSPETAKRYLTGTAQHV
                     KRRQIEMMDVTEMLNEVGYQSTMVDGVALTYSRILHSKKIPYSKAG"
     gene            complement(9687..10661)
                     /locus_tag="PLW15_03795"
     CDS             complement(9687..10661)
                     /locus_tag="PLW15_03795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT33557.1"
                     /translation="MQNTRLGELTVSRIGLGCMGMSHLYTGHSRDEASSIRTIHRALD
                     LGVSHIDTAELYGPFTNEELVGRALVGRRDSVVLATKFGLMSHVGNAGPVDSSAASIR
                     IAIEGSLRRLQTDHIDLYYQHRVDPAVPIEDVIGTLSELIAEGKIRHIGLSEAGRETI
                     RRAHAVHPIAALQSEYSLWSRDADDGTLDLLRELGIGFVPYSPLNRGLLAGTLRTRDQ
                     LEETDWRLTNPRFLPGAIESNVAIVEEIAEIGGEVGATPAQVSLAWLLAQGDDWSPIP
                     GTIRVDHLEEDLGALDVTLSTEQVHRLTDITPAVGGHHTEAQARLFDR"
     gene            complement(10703..>12233)
                     /locus_tag="PLW15_03800"
     CDS             complement(10703..>12233)
                     /locus_tag="PLW15_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOT33558.1"
                     /translation="TTIESTQGTSIVKVFDMVAGGTGATERQLSAAGLDYRAVHVHPS
                     GHAGYYPGTAMMNMKLLFAPDTGRILGGQVAGFDGVDKRLDVLATALRAELTVWDLEE
                     LELAYAPPFGSAKDPVNMAGFVASNVLLGDLVLWYAQDYPHATDGARIIDVRTPEEFS
                     VWHISGAENVPLATIRDASELWNRDEPLRLYCSVGFRSYLAHRILVQRGFTAVATLSG
                     GSETFRAWHELAPDTAEPTPAVIAYAEAVDVVATAAQRDDLRNGTGVSVDLDCSGLAC
                     PGPIMKLADTMRTLGAGDEVVVHVSDPGFALDGPAWAKRNGHELLQLTPEGPGYVATM
                     RKGGAQVAASGSSVAITPAAPKLSFVVFDGDLDKLIAAFIIANGALAMGEEVSMFFTF
                     WGLNALRRSDPPKRHKSALDSMFGAMMPRGAEKLSLSQMNMMGAGTAMIKGVMKKNGV
                     HSLPELIASAQAGGARIIGCTMTMDLLGIAESDLIDGVELGGVATFLGEAAESTTTLF
                     I"
BASE COUNT         2032 a         4149 c         4000 g         1952 t
ORIGIN      
        1 tagcgccagt cgtcgacccc gtagtcccag ccggtgtagc tctccgcgag gtagcgctgt
       61 gcgtgcagcg agtcggtgag gagcgccagt tcgccgagcc cgcggcgctt gtcgaactcc
      121 gtggcatccg gggtgatgtg aagcatcgtg gtcatccagt aggagaagtg ctgcgccttc
      181 cagatgcgct tgagggcgtt ctcggggtag gccgccatga gctcctccga gccggtcgcg
      241 aagaacgagc gcagcgcctg gttgagccag agcacgtcgc tgaaggcgag gttcatgccc
      301 ttcgcgccgg tcggcggcac cgtgtgggcg gcgtcaccca tgaggaacac gcggcccttg
      361 cgcagctcgt gggcgacgaa gctgcggaaa cgcagcacgt ccttgcggaa gatcggtccc
      421 tgcttgagcg tggtgccctc gatgccggcc tgcagcttgt cccagatctc ggcgtcgctg
      481 acggtcgtcg ggtcgagcag cgggtcgcac tggaagtaca tgcgctgaat gtgctggtcg
      541 cgagtcgaga tgagcacgaa gccctcgtcg gaacgcgagt agatcagctc gggcgcgctc
      601 ggcggcgcct cgaccagcac gccgtaccag gcgaacgggt actcgcggta gtagccgccc
      661 tgctgcgatc cgacgacggg gatgcgcgcg atcgagcccg acccgtcggc gccgaccacg
      721 acatccgcga cgatctcgaa gggttcgccc gcggcggtgg tgccgacgat gcgcggcttg
      781 tcgctctcgg cgtcctcgac ggatgtcgcc gtgtgctcga acagcggcgt gacgccgtct
      841 cgaatgcgcg cggcgatgag gtctttgagc acctcgtgct gcgggtagag gtacaccgac
      901 ttgccgacga gctcctggaa gtcgaagcgg tgcgcccggc catcgaagtg gaactcgacg
      961 ccgtcgtgct tcatgccctc gcggtagatg cggtcggaga cgcccgactc ggcgagcacc
     1021 tcggcggccg gcgactcgac gatgcccgcc ttgacggtgc cctcgatgtc ttcgcgactg
     1081 cgcgcgtcga tgacgacgga gtcgatgccg gccttgccga gcaggtgggc gagcagcagt
     1141 ccggcggggc cagccccgag aatgccgacc tgggtgcgaa tggtgttggt catggcacca
     1201 ttcagggcta tcgctcgaac cgtggcaatg cgaaacttcc atgagatgga agttcttgat
     1261 cgcgccatgt tggttctcgg atgcttcggc cccgaggcca atgcgctgac ccttacccag
     1321 atcagcgagc gcaccggact cgcgctctcg acctgccatc gaatcgtggc atccctcgtc
     1381 gcgggcggat tcctcgatcg cggcgacgac cgcaggtacc gcgtcggcac aacgctctgg
     1441 accatcgccc agcacgggcc gctcagtgac cggctgcgcg agagcgctct gcccgcgttg
     1501 gcgcgcctgt acgaggagac cggggagaac atcaccctgg ccgtgctcga caacggccag
     1561 gcgctgtacg tcgagcggct catgggcgag cgaagcatcc cgacggtgag tcgggccggn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgcgactcga tcgaccagcc
     1741 cgaccgcctg cgacgggacc tcgccgaggt gcgccagcgc ggctactcga tcacccggca
     1801 ggagatgacc gtcggcagcg gatccatcgc cgtgccgatc ctcgtcgacg gccagtgcgt
     1861 tgcggcggtc ggagtcatcg tgcacctcgc ccgcctcgac gtgaaccgtc tggtcggcac
     1921 cctcaatcgg gccgccgcga cgatcgtcac cgagctggga cggctgaagt gagcgccggg
     1981 tacactccgg gcatgagcga gaaaggcgcg gcagcccagg atgcgcccgg gccgagcgtc
     2041 accggccgcg cgctcgccgt gctcgactcg ttcgaccaga ctcaccgccg ccagtcgctc
     2101 gcggccatct cccgtcgcac cgggctgccg ctgaccacgg tgcaccgcct cgtgcacgag
     2161 ctcgagcggc acggtgcgct ggcgcgcgga gccgatggcg actacgagat cggcagcaag
     2221 atctggcgcc tcggcacgct ggcgtcggtg cacgtcgaac tgcgcgaggt ggcgctgccc
     2281 tacatggagg acgtctacca gctcgccaat gacgccgtgc agatcgcggt gctcgacggc
     2341 ctgcgcatcc tcgtcgtcga ccgcatcgcg ggctcgcgca gcatcgaggt gctctcgaag
     2401 cccggctcgc gtctgcccct gcacgcgact ggggtcggca aggtgctgct tgcctacggc
     2461 ggccaagacc ttcaggatgc ggtgctcggc tctctagacc gctacaccga cagcacgatc
     2521 accgatgcac cgacgctgaa ggcccagttg ctcacgatcc gctcccaggg cttcgcggtc
     2581 acgcgggagg aactcgcgcc gggcgcgacg tcggtggcag taccgctgcg cggtaagggc
     2641 ggcacggtga tcgcggctct cggggtggtc tcctccagcg acaacgccga gatctcgcgc
     2701 atggtgccgg tgctgcaggt gacggcggcg gcgctctcga agaagctcgc gcagtcgggg
     2761 ctgggcgagg catcccggct gcggtgaacc gaccttccgc tgaacggaat atgccctagc
     2821 cccgtcgggc gtcatgccgt tgtctgggtg ggcaccctgc tgcccacttc gaaggagaac
     2881 catggcgcgc gtcgtccgag gcaccccccg caccccgctc gagatcgtcg atggtctcgc
     2941 cgagctgggg gtcgcgacag tgcacgaggc gcaggggcgc atcggtctgc tgaatgtgcg
     3001 gctgcgcccg atcttccccg cgaagatcgc cggcaccgcg ctgaccgctg aggtcgcacc
     3061 cggtgacaac tggtcgatcc acgtcgcggt cgagcaggcc cagccgggcg acatcctcgt
     3121 ggtcacgccg acgagcccct gcgaggacgg ctacctcggc gacctgctcg ccgactcgct
     3181 cctggcccac ggcgtgcgcg ggctggtcat cgacgccgga gtgcgcgacg tggcgaccct
     3241 caccgagatg ggcttcccgg tctggtcgaa gaacatcggc gcgcagggca ccgtgaagtt
     3301 cacgcccggc aacgtgcaga cgccgatcgt gatcgccgag cagctcgtga accccggcga
     3361 cgtgatcgtg gccgacatcg acggcgtcgt cgtggtgcgc cgtgaagacg cggaggccgt
     3421 gctcgccaag ggtcgcgagc gcgaggccaa cgagaccgcc aagcgcgagc gcctgcgcgc
     3481 cggagagctg ggcctcgaca tctacggcat gcgcgacaag ctcgctgccg ccggcctcgt
     3541 ctacgttgac tacaggcccg aggactacgc cggatgacgc cgaccaactc ccagcggtcc
     3601 gtgcccggcg ccctcatgcg tggcggcacg tccaaggggc tgtacttcct ggccgacgat
     3661 ctgccgcacg accgtgcagc gcgtgacgag tttctgctcg cggcgatggg gtcgcccgac
     3721 gtgcgcgaga tcgatgggct cggcggcggg catccgctca cctcgaaggt ggccgtcgtc
     3781 agccgctcag accgcgaggg catcgacgtc gactacctgt tccttcaggt gtggcccgac
     3841 cgggccgagg tcagcgacca gcagaactgc ggcaacatcc tcgccggcgt cggtccgttc
     3901 gcgatcgagc gcggactcgt cgcccccagc ggtgacctca ccccggtcgc catcttcatg
     3961 gtgaacaccg actcgaaggc gaccgcgttc gtcgagactc ccggcggcgt cgtgaactac
     4021 gcgggcaccg cgcgcatcga cggcgtgccc ggcacgcacg ccccgatcat gatcaacttc
     4081 gaggatgtcg ccggctccag ctgcggcgcc ctgctgccca cgggcaacgc cgtcgacacc
     4141 gtgctcggcg tcgatgtcac ctgcatcgac aacgggatgc ccgtggtctg cctagcagcg
     4201 tcagacttcg gcatcacggg ctacgagacc ccggccgagc tcgaggccaa cgaggagctg
     4261 aaggcccgcg tcgaggccat ccgcctcgcg gtcggcccga tgatgaacct cggcgacgtc
     4321 acgaacaaga ccgtgcccaa gatgagcctg ctcgcccccg cccgggacgg cgggctggtc
     4381 gccactcgca cgttcatccc gcaccgcgtg cacgaatcca tcggggtgct gggcgccgtg
     4441 tccgtggcga ctgcctgcat gatcgacggc agtgttgcgg cgcgcatcgc cgggctgtcg
     4501 acggctgcca ccgacctcgt ggtcgaggtc gagcatccga ccggcttctt caccgtgcag
     4561 atggaggtcg accgctcgtc gggcgaactc gtcgtcacca agtccgcgct gctgcgcacc
     4621 gcccgcatgc tcatgtcggg cgacatctac gtcatcgatt ccggagaagc cgcatgatca
     4681 tcgacatcca cggtcactac acgaccgcgc ccgaggccca caaccagtgg cgcatcgacc
     4741 agttggccgc gtacgccgcc ggccagccga gccccgccta ccctgagatc tcggatgaca
     4801 cgatccgggc gtcgatcgag gagaaccagc tcaagctgat caccgagcgc ggcagcgacc
     4861 tcacgatctt ctcgccccgc gcgtcggcga tgggacacca cgagggcgac cagcgcatca
     4921 gcctcgagtg gacggtggcc tgcaacaacc tcatcaagcg cgtcgtcgac ctcttccccg
     4981 acacgttcgt gggcgtcggc cagttgccgc agtcgccggg ctccgacctc accgcgacca
     5041 tcgcggagct cgagcgcgtg gtcaacgagc tcggcttcat cggcgtgaac ctcaaccccg
     5101 accccagcgg tggtctctgg cagaccgtgc cgctcacgga caggttctgg tacccgctct
     5161 acgagaagat ggtcgagctc gacgtgcccg cgatggtgca cgtgtcggcg agttgcaacc
     5221 cgaacttcca cgcgaccggc gcgcactaca tgaatgccga caccaccgct ttcatgcagc
     5281 tgatccaggg cgacctgttc gctgacttcc cgaccctgcg cctgatcatc ccgcacggtg
     5341 gcggagcggt gccgtaccac tggggtcgct accggggtct ggccgacatg ctcaagcagc
     5401 ccgatctcag cacccatgtc atgaacaacg tcttcttcga cacctgcgtc taccaccagc
     5461 cgggcatcga tctgctcgtg aaggtcatcg acaacaagaa catcctgttc ggttcggaga
     5521 tgatcggcgc cgtgcgcggc atcgacccga cgaccggcca gtacttcgac gacacgaagc
     5581 gctacatcga cgcggcgcag atcagcgacg cccagaaggc gagcatcttc gagagcaacg
     5641 cgcgtgcggt gttcccgcgg ctcgatgcga tcctcgcgga gcgaggccga tgagcgccgc
     5701 ccccgccttc gtcaaggatg ccgactggct cgactgggat cccgatccca ccaagcccgc
     5761 cttcgtcgtg cccgctggct cggtcgatgc gcactgccac gtgttcggcc cgggagacat
     5821 cttccccttc gcgcccgagc gcaagtacac cccgtgcgat gcgagccgag agcagctgtt
     5881 cgcgctgcgt gacttcctcg gcttcgacaa gaacgtcgtc gtgcaggcca cctgccacgg
     5941 cgctgacaac tcggcgctgc tcgacgtgct cgagcactcc gaggatcgcg ctcgcggagt
     6001 ggtcaccgtc aagcccgaca tcgaggctcg cgagctcgcc cgcatggacg acctcggcgt
     6061 gcgcggcgtg cgcttcaact acgtcaagcg cctggtcgac ccgaagcccg acgactacta
     6121 ccgtgcgatc gtcgacaaga tccgcccgct cggctggcac gtggtgctgt acttcgaacc
     6181 cgccgatctc gccgagaagc gcgacttctt cgcgagcctt ggcgtgccga tcgtcgtcga
     6241 ccacatgggc cgccccgacg tgacgaaacc cgtcgatgga cccgagttcg ccgcgtacct
     6301 cgacttcgcc cgcacggcgg atgtctggac caaggtcagc tgccccgacc gcctcaccgt
     6361 gtcgggctcg ccccactacg tagacgtggt ccccttcgcg agggcgatcg tcgaggagtt
     6421 cagcgaccgg gtgctctggg gcaccgattg gccgcatccc aacatgaaga aggagatgcc
     6481 ggatgacggc atcctcgtcg actacgtgcc gtctatcgcg acgacggtcg ccctccagga
     6541 gaagctcctg gtcaccaacc ccaccaccct ctactggagc tgaccatgac gaaaccggat
     6601 ttccaccagt tcgacgacat ccccggcacg acgatcttca cgatcgagaa ggcccgcgag
     6661 ggcttcgcgc tcaaccagtt ctgcatgagc ctgcgcaagg cggagaaccg ggagcggttc
     6721 ctcgccgaca accgcgccta cctcgacgag tgggacatga ccgaggagca gaagcaggcc
     6781 gtgatcgacc gcgacttcca ggcgatgctc gaccacggtg gcaacgtgta tttcctgtcg
     6841 aagatcttcg cctctgaggg gctgtcctac gtgcaggccg tcagcaccat gaccgacatg
     6901 aacgtggagc agtaccaggc gatgatgctg gccggcggcc gcaacgtcga aggctggcgc
     6961 agcaagaaag agcgaggaga agcctgatgg cacgcatcac cgctggggtc agcaccagcc
     7021 acgttcccgc cgtcggcgcg gcggtcgaca tgggtcgcac cgaggagccg tactggaagc
     7081 cgatgttcga gggctacgag ttctccaaga agtggatggc agaccacacc ccggatgtcg
     7141 cgatcatcgt ctacaacgac cacgcgtcgt acttcggcat ggagatcatg ccgaccttcg
     7201 cgatgggcat cgccgactcg ttcgcgatct gcgatgaggg ctacggaccg cggcccgtgc
     7261 ccgtggtcga gggcgcgccc gagctcgcga cgcacctcgc ggcatccctg atcctcgacg
     7321 acttcgacat gaccgtgctc aacgtgctcg aggtcgacca cggactcacc gtgccgatgt
     7381 ctctcatgtt cggcactccc gacaagtggc ccaccaaggt gatccccctg ccggtcaacg
     7441 tggtcatgta cccgcagccg tcgggcgagc gctgcctgtc gctcggtaag gcgatccgtc
     7501 gggccgtgca acagttcgac gaggatctcg acgtgcagat ctggggcacc ggcggtatga
     7561 gccaccaaat acagggagct cgggcgggcc tcatcaacct gccgtgggac atccagttca
     7621 tggacgacct catcaaccag cccgaggtac tgcagtacaa gccgcacctc gagtacatga
     7681 tcgaggccgg ctccgagggc gtcgagctca tcatgtggct catcatgcgc ggcgcgctcg
     7741 gtgaccaggt cgacgaggtc taccgtttca accacgtgcc ggcctccaac acctcggtcg
     7801 gccacctgat cctgaccccg aaggagatct gagcgtgacg gtcaacctcg cggtcgtggg
     7861 cctcggtgcc ttcggccaga agcacctcga tgccctcgcg cagatcccgg acgcggtcat
     7921 ccattcggtg gctcattcga agatggatgt cgcgcaggag gtcgccgcga agtacggcgc
     7981 cgcgcacgcc ttcaccgact actcggcgct gctcgcccag cccgacctcg acggcgtgct
     8041 gctggcgacc cccacccaga tgcaccacgc gcagaccatc gaggcactgc gggcgggcaa
     8101 gcacgtgatg gtcgagatcc cgatggccga ctcgctcgcg ggcgtgaagg agatcgtcga
     8161 cgtgcaggcc gagacgggcc gcatcgcgat ggccggccac acccgccggt tcaacccgtc
     8221 gcaccagtac ctgcacaaga agatcgtcgc cggcgagttc gccatccagc agatggacgt
     8281 gcagacctac ttcttccgtc gcaccaacat gaacgcgctc ggccagccgc gctcgtggac
     8341 cgaccacctg ctctggcacc acgccgccca caccgtcgac ctgttccagt accagacggg
     8401 ggagcggatc gtgaaagcca acgcgatcca ggggccgaag catcccgagc tcggcatcgc
     8461 catggacatg tcgatccagc tgaagaccga gaccggcaag atctgcacgc tcagcctgtc
     8521 gttcaacaac gacgggccgc tcggcacctt cttccgctac atcggcgaca ccggcaccta
     8581 catcgcccgc tacgacgacc tcgtcacggg caaggaggag ccgatcgacg tgtcacacgt
     8641 ggatgtgtcg atgaacggga tcgagctgca ggaccgcgag ttcgtggctg cgatcgtcga
     8701 aggccgcgag cccaactcga gcgtcgccca ggtgctgccg tgctacgtgg tgctcgacga
     8761 actgcagagg cagctggagt actgaccagc gctggttgag tagccgacga agtcggcgta
     8821 tcgaaaccgg gattgcggcc gatttcgata cgcgcttcgc gctactcaac cggccttgct
     8881 gtaggggatc ttcttgctgt gcaggatgcg cgagtaggtg agtgcgacgc cgtcgaccat
     8941 ggtcgactgg tagccgacct cgttcagcat ctcggtgaca tccatcatct cgatctggcg
     9001 gcgcttgacg tgctgggcgg tgccggtgag gtagcgcttg gccgtctcgg gtgagctcga
     9061 gtcgaactcg cgcaggatct cctcgtaggc ccactgctgc agtcccgtgc tctcggccgc
     9121 ccacagggtg tcgatgagca cggccgccat gcccttggcg aggatgctcc gcagcagctt
     9181 gcgggccgcc gcctctcccg gcacgtccga gacgtactcg aggttcatgc cgtacggctc
     9241 gagaaggtcc acgaaacgtc gcgctcccgg tcccgcgacc cccatgggca ccttctcggc
     9301 gagcccgggg accggcttca tgatggccac gtcggcgaac actccgtcgc cgaagagccc
     9361 ggcgagtgcc ttcttcagcg ctggcgttcc ggtgttgagg tcggcgtaga cagtgccggc
     9421 tcccagcagc ggggccacct gctcggccac cttcttggcg gcggtcgacg agttgatcga
     9481 gatgacgatg tcggcgccgg tgacgactgc ggcgacatcc ggtgcgaggg gtactggtgg
     9541 tgtttcagga acgacggggt cgaacccgat gacgtcggca cccgccgcct gcagcccggc
     9601 cgcgatcagc gatcctgctt cgcccagccc gatgacaccg attcgcacac gagagctcat
     9661 gcacccattc tcccgcggcg cgagggtcag cggtcgaaca gccgcgcctg cgcctcggtg
     9721 tggtggccgc cgacggcggg ggtgatgtcg gtgagccgat gcacctgctc ggtgctgagc
     9781 gtcacatcga gtgcaccgag gtcctcctcg aggtgatcga cgcggatcgt tccggggatc
     9841 ggcgaccagt cgtcgccctg cgcgagcagc caggcgagcg agacctgcgc cggtgtcgcg
     9901 ccgacctcgc cgccgatctc ggcgatctcc tccacgatgg cgacgttgga ttcgatggcg
     9961 cccggcagga atcgcggatt cgtcagccgc cagtcggtct cctcgagctg gtcccgggtg
    10021 cgcagggtgc ccgcgagcag gccgcggttg agcggcgagt agggcacgaa gccgatgccg
    10081 agctcacgca gcagatcgag cgtgccgtcg tcggcgtccc ggctccagag cgagtactcc
    10141 gactgcaacg cggcgatcgg atgcacggcg tgcgcacgac ggatcgtctc acgccccgcc
    10201 tccgagagtc cgatgtggcg aatcttgccc tcggcgatga gttcactcag cgtgccgatg
    10261 acatcctcga tgggcaccgc cgggtcgacg cggtgctggt agtagaggtc gatgtggtcg
    10321 gtctgcaggc gccgcaacga cccctcgatc gcaatgcgga tgctcgcagc cgagctatcc
    10381 accgggccgg cgttgccgac gtgcgacatc agcccgaact tggtggccag tacgaccgag
    10441 tcgcgacggc cgaccagcgc gcggccgacg agctcctcgt tggtgaacgg tccgtagagc
    10501 tccgcagtgt caatgtggga gacgcccagg tcgagcgctc gatggatcgt gcggatcgag
    10561 gatgcttcgt cccggctgtg tcccgtgtag agatgtgaca tccccatgca gccgaggccg
    10621 atgcgcgaga cagtgagctc gccgaggcgc gtgttctgca tgtgtgtact cccgattatc
    10681 agactatcgg gagcccgcgg cactagatga acagcgtcgt cgtcgactcc gccgcctccc
    10741 cgaggaaggt cgcgactcca cccaattcca ccccatcgat caggtcggac tcggcgatgc
    10801 ccagcaggtc catggtcatg gtgcagccga tgatgcgcgc cccgcccgct tgtgccgagg
    10861 cgatcagctc gggtagcgag tgcacgccgt tcttcttcat gacgcccttg atcatcgcgg
    10921 tgcccgcgcc catcatgttc atctgcgaga gggagagctt ctcggcaccc cggggcatca
    10981 tcgcgccgaa catcgagtcg agcgcgctct tgtggcgctt cggcgggtcc gagcgacgca
    11041 gggcgttgag accccagaag gtgaagaaca tcgagacctc ctcgcccatg gcgagcgcgc
    11101 cgttggcgat gatgaacgcg gcgatgagct tgtccagatc accgtcgaag acgacgaacg
    11161 acagcttcgg tgcagcgggt gtgatcgcga cggagctccc gcttgccgcg acctgcgcac
    11221 ctcccttgcg cattgtggcg acgtaacccg ggccctcggg agtcagctgc aggagttcgt
    11281 gtccgttgcg cttggcccaa gccgggccat ccaacgcgaa gccggggtct gacacgtgca
    11341 cgacgacctc gtcaccggcg ccgagcgtcc tcatggtgtc ggcgagcttc atgatcggtc
    11401 ctgggcaggc aagtccggag caatccagat cgaccgagac gccggtgcca ttgcgaaggt
    11461 cgtcgcgctg ggccgcggtc gcaaccacgt cgaccgcttc cgcgtaggca atgacagcgg
    11521 gcgtgggctc ggctgtgtcg ggggcgagtt cgtgccatgc ccggaaggtt tcggaaccgc
    11581 cggagagggt cgcgacggcg gtgaaccccc gctgcacgag aatccggtgg gcgaggtagc
    11641 tgcggaaacc caccgagcag tagagacgca gtggctcgtc gcgattccag agctcactgg
    11701 catcccgaat cgtcgcgagc ggcacgttct cggcgccgga gatgtgccag acggagaact
    11761 cctccggggt gcgcacgtcg atgatgcgcg ctccgtctgt ggcgtgcgga tagtcctggg
    11821 cataccagag cacgaggtcg ccgagcagaa cattgctcgc gacgaacccg gccatgttca
    11881 ccgggtcctt ggccgaaccg aacggcggcg cataggcaag ctcgagctcc tcgaggtccc
    11941 acactgtgag ttccgcccgc agcgccgttg ccagaacgtc gaggcgcttg tcgaccccgt
    12001 cgaaaccggc gacctggcca ccgaggatgc gccctgtgtc cggcgcgaac agcagcttca
    12061 tgttcatcat ggccgtgccc gggtagtagc cggcatgacc ggatggatgc acgtgcacag
    12121 cccggtagtc aagcccggca gcgctcagct gacgctcggt cgcgcccgtg ccgccggcaa
    12181 ccatgtcgaa gaccttgacg atcgacgtgc cctgggtcga ctcgatcgtc gtg
//
LOCUS       DAOSOU010000043        12177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5204_length_12177_cov_6.086702, whole genome shotgun sequence.
ACCESSION   DAOSOU010000043 DAOSOU010000000
VERSION     DAOSOU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5204_length_12177_cov_6.086702"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PLW15_03805"
     CDS             complement(<1..1068)
                     /locus_tag="PLW15_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOT33559.1"
                     /translation="MTTTQEPQTAAVEPTRGTRFTGWAANYIDERTSISGLVKEVGRK
                     IFPDHWSFMLGEVALYSFIVILLSGTFLTFFFQASMVPVIYDGSWVPLKGIEMSAAMD
                     STLNLSFDIRGGLLMRQVHHWAALLFVASIGLHMLRVFFTGAFRKPREINWIIGFVLF
                     ILAMLEGFTGYSLPDDLLSGNGLRIIDGMVKGVPIVGTWISYLVFGGEFPGTDIVGRL
                     YTVHILLAPAILVALLGVHMLLLIVNKHTQFAGPGRTNDNVVGVPIMPVFAAKAGGFF
                     FIIFGVIVLIASFFQINPIWVYGPYDPSPVSAGTQPDWYIGFADGALRLVPPGWEVEL
                     GVFTLSLNIMVPLLILPIFLAL"
     gene            complement(1065..2141)
                     /locus_tag="PLW15_03810"
     CDS             complement(1065..2141)
                     /locus_tag="PLW15_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOT33560.1"
                     /translation="MAQDHDGATGGDAAGSTVEKYGANPPSAGTAVVVADRIPNPGFE
                     PYRPRVTDLDEAKERQQERRVAGFFILSIVGTVAAIVAYIIFPIVPGDLNSVRFNNLF
                     LGIGITLALLSVGIGAIHWSKSLMDGHDMVEQRHGTRGSDETRAKAVEVFTLGNKESG
                     FGRRTLIRRTLIGALVVTPLPAIVLFRDLAPAEDPVPLLQHTLWKKGTRLTRDPSGTP
                     IRASEVTLGSAFHVIPEGLNELEDKLEQKAKAAVLLMRLKPEDLQVSPGREDWNYAGI
                     VAYSKICTHVGCPVALYEQQTHHLLCPCHQSQFDITREAKVIFGPAKRPLPQLPITVD
                     AEGYLVARSDFTEPVGPSFWERNT"
     gene            complement(2193..2996)
                     /locus_tag="PLW15_03815"
     CDS             complement(2193..2996)
                     /locus_tag="PLW15_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOT33561.1"
                     /translation="MSKKSEAPSNRKAGRRSPLATVALLTIGLLSTGGAYALFTTSAT
                     AETSAAADAQIEEGGKLFAANCATCHGMALQGTSQAPSLIGVGAAAVDFQVGTGRMPL
                     AATGPQGEQKPVQFTAEQISALSAYVASRGTGPGIPDEKYLQGDGDAAVGAELFRINC
                     AMCHNVAGAGGALTEGKFAPPLSGVEPAYIYEAMVTGPQNMPVFNDHNISPEDKANII
                     TYLKFLDNTTSVGGFGLGDIGPVSEGLFIWIFGLGAIVAITVWLTAKSN"
     gene            complement(3017..3691)
                     /locus_tag="PLW15_03820"
     CDS             complement(3017..3691)
                     /locus_tag="PLW15_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HOT33562.1"
                     /translation="MTFGATEIDHNGRVTTASLSRSITAPSVQRPNIVAVGTIVWLGS
                     EVMFFAGLFAIYFTLRSMSPELWSEQTAKLNVTYSLINTIILVLSSFTCQAGVFAAER
                     NQPRRTGTALQFWKWGTVEWFYLTYFMGAAFVVGQIFEYANLVSEHIGLSSDSYGSVF
                     YLTTGFHGLHVTGGLIAFLLVIGRIFAVKVFTHKEATTAIVVSYYWHFVDVVWIGLFL
                     VIYVLK"
     gene            3733..4788
                     /gene="trpD"
                     /locus_tag="PLW15_03825"
     CDS             3733..4788
                     /gene="trpD"
                     /locus_tag="PLW15_03825"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOT33563.1"
                     /translation="MSPERTWPIILSTLVEGDDLSISDAEWAMQQVMAGEATPAQLAG
                     LLVALRVKGETVDEIVGFRDAVLANAQPLPVSPDVLDIVGTGGDPYGAVLNISSVASI
                     VAAAAGVPVVKHGNRGASSASGASDVLSALGLNLDITPDRVAEVLAETGITFAFAALF
                     HPGFRHAGPTRKELGISTLFNILGPLCNPARPNASAVGVASLERVPLVVGVFQTRGAT
                     ALVYRGDDGIDKLTTTGHSHIWEVSRGFVTEHDLDPADLGIPRADIQSLLGADAAHNA
                     SVARAVLVGEPGPVRDIVLLNAAGGLAAYELSREPELLQEPIVQRLATQLERAASVID
                     SGAASAKLDEWVAATNR"
     gene            complement(4829..6352)
                     /gene="glpK"
                     /locus_tag="PLW15_03830"
     CDS             complement(4829..6352)
                     /gene="glpK"
                     /locus_tag="PLW15_03830"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOT33564.1"
                     /translation="MTTDTPKFVLSLDQGTTSTRAMIFDHAGAVVTVGQLEHRQHFPQ
                     AGWVEHDPVEIWDNTREVIGQALGKANLTRHDIAAVGITNQRETTVVWDRTTGLPVYN
                     AIVWQDTRTQSIVDRIAAGDVDRFKEKVGLPLATYFSATKIAWILENVQGARARAEAG
                     ELAFGTMDTWLLWNLTGGIHGGVHATDVTNASRTLLMDLATCAWDPELLAAFDVPAAL
                     LPEIRSSSEIFGTVELQNLLREVPVAGMLGDQQAATFGQAAFGVGESKNTYGTGNFLI
                     VNTGEDIVHSRNGLITTVAYKLGDGPTHYALEGSIAVTGSLVQWLRDNLGIISKASDV
                     EALARTVDDNGNVYFVPAFSGLFAPHWRPDARGAILGLTRFSNKGHIARAALESTAFQ
                     TREVLDAVNADAGVDLTELKVDGGMIANSELMQFQADLLGVPVIRPVVAETTALGAAY
                     AAGLAVGFWSGLDELRANWREGARWLPSMPEEERERLLRNWRKAVSKTLDWVDDDVR"
     gene            complement(6387..6746)
                     /locus_tag="PLW15_03835"
     CDS             complement(6387..6746)
                     /locus_tag="PLW15_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOT33565.1"
                     /translation="MPLSECCAPIIDGRATAPTAERLMRSRFTAFALGDADYLLKSWH
                     PTTRPRSLQLDPDLRWYRLDIIGRSKGALLDTEGTVEFVAHYRSPAGSGEQHENSRFL
                     RDAGEWRYLAAVGPPGR"
     gene            complement(6784..7146)
                     /locus_tag="PLW15_03840"
     CDS             complement(6784..7146)
                     /locus_tag="PLW15_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33566.1"
                     /translation="MAAPTGPSDRQLARERGESLLDQVAERFVGVPGVQAGRMFNGRG
                     LSVDDSIFVFINREGRMVAKLPEPEAQARVAAGDADVVSMGTRTMREWVSFAQPTELD
                     TWVAVARDAHRYVSALTP"
     gene            7266..8693
                     /gene="argG"
                     /locus_tag="PLW15_03845"
     CDS             7266..8693
                     /gene="argG"
                     /locus_tag="PLW15_03845"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT33567.1"
                     /translation="MSKVLSSLPVGERVGIAFSGGLDTSCAVAWMREKGAVPCTYTAD
                     IGQYDEPNIGEVPARAKEYGAEIARLVDGRAALVEEGLVALACGAFHIRSGGKTYFNT
                     TPLGRVVTGTLLVRAMKEDDVDIWGDGSTYKGNDIERFYRYGLLANPRLRIYKPWLDA
                     DFVGELGGRTEMSEWLVARGFPYRDSTEKAYSTDANIWGATHEAKNLEELSTGLDIVD
                     PIMGVASWRDDVEIATEEVTVRYEAGRPVAINGVEFTDPVALVLEANAIGGRHGLGAS
                     DQIENRIIEAKSRGIYEAPGMALLHITYERLLNAIHNEDTIANYHAEGRRLGRLMYEG
                     RWLDPQSLMLRESLQRWVGSAVTGEVTLRLRRGDDYTILNTTGPNLSYHPEKLSMERV
                     GDAAFGPGDRIGQLTMRNLDIADSRSRLEQYAAIGLIGGPTAELVGELEVGAANQITG
                     GADDTALDRATDAANEASAFELGTD"
     gene            complement(8727..9509)
                     /locus_tag="PLW15_03850"
     CDS             complement(8727..9509)
                     /locus_tag="PLW15_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT33568.1"
                     /translation="MSRTYVVTGAASGIGKATTELLLERGHTVIGVDIRDADVTVDLS
                     TAEGRAALVEKVTALSGGTIDAVIANAGLATPTAITVAVNYFGTVATLEGLRPLLVNS
                     PAPRAVATASMASLFPPDDALLTALLAGDEKAALARGAELEAGGPQLGSLIYGTTKRA
                     LALWIRRHAATSDWAGASIPLNGVAPGVVETPMTADLIATPEQREQITTMVPMPLNGI
                     FEPRSVAYLLAWLTSEENAHLCGQIVFIDGGSDVIIRGDSTW"
     gene            complement(9602..10546)
                     /locus_tag="PLW15_03855"
     CDS             complement(9602..10546)
                     /locus_tag="PLW15_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33569.1"
                     /translation="MSDETTAVPTVEEGLVEVSPTEEKAPAARPKRLALILLGIGLAL
                     TIGAVIFLVVQLLSANSRIAEQNRELDQQRELIEKKETFGAAMAQLLETASLLDGSLM
                     SSLVPLDSYESIARLAWSHRWSAGSVEADTRRIQSEEATLKDLVSEASAQAGSNTSGT
                     TYEAVIDDLGGGHVTTVIDDADTLCEADVLACVVYDDPFAVHFDAADAGQPYMTDWLR
                     TGIAYHEFAHVLQMTNPDATDTALEAFGGDPETMADCFALTYLSGWTLDHRVYVSSYE
                     YWDVSIGYGYTCDETQAQAVRDWYGQLGVHLRPISQSR"
     gene            complement(10599..11456)
                     /locus_tag="PLW15_03860"
     CDS             complement(10599..11456)
                     /locus_tag="PLW15_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT33570.1"
                     /translation="MEGKWRWILVTAIAPIAWGSNYFVTRHFLPPDAPLWGALLRALP
                     AGLILLLLARRLPRGSWWWRSLVLGTLNVGAFFVLIYVVSQRLPSGIASTIMATSAAA
                     LLLLAWPLLGERPTRFGVIGAAVGLLGVGVLLFEGGLTVDGWGVIASLAAMVLSSIGF
                     ILTKRWGAEVPLVALTSWQLIGGGLVVLPFAVILEGAPPAGEPGAVLGYVYVAVIATA
                     LAFIAWFAGLRHLPAGTVGLVGLLNPVTGVLLGTLLAGESFGIRQAVGTLLVITGVVV
                     GQRRRSAPT"
     gene            11476..11988
                     /locus_tag="PLW15_03865"
     CDS             11476..11988
                     /locus_tag="PLW15_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT33571.1"
                     /translation="MEDSRGDIGDRVSRIQSEWRRERPDVDVQPQGIIGRVHRLAAWL
                     TDELTVVYGRYGLGEGEFDVLAALRRAGHPFERAPGELADHTMVTTGAMTKRIDRLVA
                     RGLVERREVEQDGRRRIVALTLAGRELIDAAFTEHMRNERRLVDELAADDQAALEAIL
                     TRWLAAGPTR"
     gene            complement(11985..>12177)
                     /locus_tag="PLW15_03870"
     CDS             complement(11985..>12177)
                     /locus_tag="PLW15_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33572.1"
                     /translation="RTIAWMRLINDARTEPFETSSDFADHRPALLRPDRLDDHYSAGR
                     LASDEARTSFVEPDLTPLP"
BASE COUNT         2128 a         3822 c         4177 g         2050 t
ORIGIN      
        1 cagcgcgagg aagatgggca gaatcagcag cggaaccatg atgttcagcg acagcgtgaa
       61 caccccgagc tccacctccc agcccggcgg cacgagtcgc aatgcaccat cggcgaagcc
      121 gatgtaccag tcgggctgcg ttcccgcgga aaccggagac gggtcgtacg gcccatacac
      181 ccagatcggg ttgatctgga agaacgaggc gatgagcacg atcacgccga agatgatgaa
      241 gaagaaccca ccggccttgg cggcgaacac cggcatgatc ggcacgccca caacgttgtc
      301 gttggtgcgg ccggggccgg cgaactgtgt gtgcttgttc acgatgagaa gcagcatgtg
      361 cacgccgaga agggcaacga ggatcgcggg cgccaagagg atatgcaccg tgtacaggcg
      421 gccgacgatg tcagtaccgg ggaactcgcc accgaacacg aggtaggaga tccaggtgcc
      481 gacgatcggc acacccttga ccatgccgtc gatgatgcgc agaccgttgc cggagagcag
      541 gtcgtcgggg agcgagtagc ccgtgaagcc ctcgagcatc gccaagatga acagcacgaa
      601 gccgatgatc cagttgatct cgcggggctt gcggaacgcg cccgtgaaga acacgcgcag
      661 catgtgaagg ccgatggatg ccacgaacag caacgccgcc cagtggtgca cctggcgcat
      721 caggaggccg ccgcggatgt cgaacgacag gttgagcgtc gagtccatcg cggccgacat
      781 ctcgatgccc ttcagcggaa cccacgagcc gtcgtagatg acgggaacca tggatgcctg
      841 gaagaagaac gtgaggaacg tgccggagag aaggatgacg atgaagctgt agagagcaac
      901 ctcgccgagc atgaaggacc agtggtcggg gaagatcttg cgcccgacct ccttgaccaa
      961 gccagaaatg ctggtgcgct cgtcgatgta gttcgcggcc cagccggtga accgagtacc
     1021 gcgagtgggc tcgacggccg cggtctgcgg ctcctgcgtg gttgtcatgt gttgcgctcc
     1081 cagaagcttg gtccaactgg ttcggtgaag tcgctgcgtg cgacaaggta accctctgca
     1141 tccaccgtga tgggcagctg ggggagggga cgcttggcag gcccgaagat gaccttcgcc
     1201 tcgcgcgtga tgtcgaactg cgactggtgg caggggcaga gcaggtggtg ggtctgctgc
     1261 tcgtacagtg ccacggggca tccgacgtgg gtgcagatct tggagtacgc gacgattccc
     1321 gcgtagttcc agtcctcgcg accgggggag acctgcagat cctcgggctt gaggcgcatg
     1381 agcagcactg ctgccttcgc cttctgctcg agcttgtcct cgagctcgtt gagcccttcg
     1441 gggatcacgt ggaaggcgga tcccagagtg acctcggagg cacggatcgg ggttccggat
     1501 gggtcacggg tcaggcgggt gcccttcttc cacagcgtgt gctgcagcag aggcaccggg
     1561 tcctcggcag gggcaaggtc gcggaagagg acgatcgcgg gaagcggtgt caccaccaga
     1621 gcgccgatga gggtcctgcg gatgagggtg cgccgcccga atccggactc cttgttgccg
     1681 agggtgaaca cctcgaccgc cttcgcgcgg gtctcatccg aaccgcgggt gccgtggcgc
     1741 tgctcgacca tgtcgtggcc gtccatcagc gactttgacc agtggatggc accgatgccg
     1801 acgctcagca gtgcgagcgt gatgccgatg ccgaggaaga ggttgttgaa gcgcaccgag
     1861 ttgaggtcgc ccggaacgat cgggaagatg atgtaggcga cgatagccgc gaccgttccg
     1921 acgatcgaca ggatgaagaa ccctgcgacg cggcgctcct gctggcgctc cttcgcttcg
     1981 tccaggtcgg tgacgcgggg gcggtacggc tcgaagcccg ggttgggaat gcggtcagca
     2041 acgaccacgg ccgtgcccgc cgaaggcggg ttcgcgccgt acttctcgac cgtggaacct
     2101 gcagcgtcgc cgccggtggc cccgtcgtgg tcctgtgcca ttgaattccc ttcagacttt
     2161 cgaactgtgt tcggttgccg cgagacagcc ggttagttgg acttggccgt gagccatacc
     2221 gtgatggcca cgatcgctcc gaggccgaag atccagatga acaggccctc agataccggg
     2281 ccgatgtcgc ccagtccaaa accgccgacc gatgtcgtgt tgtcgaggaa cttcaggtag
     2341 gtgatgatgt tggccttgtc ctcaggggag atgttgtggt cgttgaacac gggcatgttc
     2401 tgcggcccgg tgaccatggc ctcatagatg taggcgggct caacgccgct cagcggaggg
     2461 gcgaacttac cctcggtgag agcgccgcca gcgccagcga cgttgtggca catcgcacag
     2521 ttgattcgga acaattccgc acccactgcg gcatcgccgt caccctgaag gtacttctcg
     2581 tccgggatgc ccgggccagt tccgcgagac gcgacgtagg cagagagagc ggagatctgc
     2641 tcggcggtga actgcacggg cttctgctca ccctgcgggc cggttgcggc aagcggcatc
     2701 cgcccggtac cgacctggaa gtcgacggcg gccgccccga caccgatcag actgggcgcc
     2761 tggctggtgc cctgaagggc cattccgtga caggttgcgc agttggccgc gaagagcttg
     2821 ccgccctcct cgatctgggc atctgcggct gcgctcgtct cagcggtcgc gctggtggtg
     2881 aacagcgcgt aggcgccgcc cgtgctcagc agaccgatcg tgagcagtgc gacggtggcg
     2941 agcgggctgc ggcgacctgc cttgcggttc gagggtgcct ctgacttctt cgacatgttc
     3001 tgtttcgttc cgattctcac ttgagtacgt agatgaccag gaacagcccg atccacacca
     3061 cgtcgacgaa gtgccagtag tacgagacga cgatcgcggt ggtcgcctcc ttgtgggtga
     3121 acaccttgac tgcgaagatt cggccgatga ccagcaagaa tgcgatcaga ccacccgtca
     3181 cgtggaggcc gtggaaacct gtcgtgaggt agaacaccga gccgtaggag tcactcgaga
     3241 gtccgatgtg ctcgctcacg agattcgcgt actcgaagat ctggccgacg acgaatgccg
     3301 cgcccatgaa gtaggtgagg tagaaccatt cgaccgttcc ccacttccag aactggagtg
     3361 cggtgcccgt gcgtcggggc tggttgcgct cggccgcgaa cacgcccgcc tggcaggtga
     3421 acgacgagag cacgaggatg atcgtgttga tcagcgagta cgtgacgttg agctttgcgg
     3481 tctgctccga ccacaactcg ggagacatcg atcgcagtgt gaagtagatg gcaaacaggc
     3541 ctgcgaagaa catgacttcg ctgccgagcc acacgatcgt gccaacggcc acgatgttgg
     3601 gccgctggac ggatggcgct gtgatcgagc gcgataggga ggctgtggtc actcttccat
     3661 tatggtcgat ttccgtcgcg ccaaatgtca ttccgggggg tcagtcggcg cccgatcgtt
     3721 aggatttggg tcatgtcacc cgagcgcacc tggccaatca tcctgagcac cctcgttgag
     3781 ggcgatgacc tgtcgatcag cgatgcggaa tgggccatgc agcaggtgat ggcgggggag
     3841 gcaaccccgg ctcagctggc aggcctcctc gtggcgctgc gcgtgaaggg tgagacggtc
     3901 gacgagatcg tcggcttccg cgatgccgtg ctcgccaatg cgcagcccct gccggtgtcc
     3961 ccggacgtgc tcgacatcgt cggcacgggc ggtgacccct acggggcggt gctgaacatc
     4021 tcatcggtcg cgtcgatcgt cgccgccgcg gccggagtgc ccgtggtcaa gcacggcaat
     4081 cgcggcgcca gctcggcttc gggcgcgtcc gatgtgctgt cggccctcgg tctcaatctc
     4141 gacatcacgc ccgaccgtgt ggccgaggtg ctggcggaga ctggcatcac cttcgcgttc
     4201 gccgccctct tccaccccgg attccgtcac gccggcccta cgcgcaagga gctgggcatc
     4261 tcgacgctgt tcaacatcct cgggccgctg tgcaaccctg cgcggcccaa cgcctcagcg
     4321 gttggtgtcg ccagtctgga gcgggtgcca ctggtcgtcg gggttttcca gacgcgcggt
     4381 gcgaccgcac tggtctaccg cggcgacgac ggcatcgaca agctcacgac gaccggccac
     4441 agtcacatct gggaggtctc ccgcggattc gtgaccgagc acgacctcga tcccgccgat
     4501 ctcgggatcc cgcgcgccga catccagtcg ctgctgggag cggatgccgc gcacaacgcg
     4561 tcagtggcgc gtgcggtgct cgtgggggag ccgggcccgg tgcgcgacat cgtgctgctc
     4621 aacgccgcag gcgggcttgc agcgtacgag ttgtcgcgcg agccggagtt gctgcaggag
     4681 ccgattgttc agcgactcgc gacgcaactg gagcgggcgg catccgtcat cgactccggg
     4741 gcagcatcgg caaagctcga cgagtgggtc gctgcgacga accggtagtc gatctcgacg
     4801 agctcgatca ccgggcccga tcgttgggtc agcgcacgtc gtcatccacc cagtcaaggg
     4861 tcttcgagac tgctttgcgc cagttgcgca gcagacgctc gcgctcctcc tcgggcatcg
     4921 acggcagcca gcgcgcaccc tcccgccagt tggcgcggag ctcatcgaga cccgaccaga
     4981 atccgacggc taggccggca gcgtaggcag cgccgagtgc cgtggtctcg gcgacgacgg
     5041 gacggatgac cggcacgccg agcaggtcgg cctggaactg catcagttcg gagttcgcga
     5101 tcattccgcc gtcgaccttg agctcggtga ggtccacgcc tgcgtccgcg ttgacggcat
     5161 ccaatacctc gcgagtctgg aacgcggtgg actcgagcgc cgcgcgagcg atatgtccct
     5221 tgttggagaa ccgcgtgagg ccgaggatcg cgccgcgcgc atccgggcgc cagtgtggcg
     5281 cgaacaggcc ggagaaggcg ggcacgaagt agacgttgcc gttgtcgtcc acggtgcgcg
     5341 cgagcgcctc cacatctgat gccttcgaga tgatgccgag attgtcccgc agccactgca
     5401 ccagtgaccc ggtcacggcg atcgagccct cgagggcgta gtgggtcggc ccatcgccga
     5461 gcttgtaggc gacggtggtg atcaggccgt tgcgggagtg gacgatgtcc tcgccggtgt
     5521 tcacgatgag gaagttgccc gtgccgtagg tgttcttcga ttcgccgacg ccgaacgcgg
     5581 cctggccgaa tgtcgcagcc tgctggtcgc ccagcattcc tgcgaccggg acttcgcgca
     5641 gcaggttctg cagttcgacc gttccgaaga tctccgatga gctgcgaatc tcggggagca
     5701 gggcggcggg tacgtcgaag gcggcgagaa gctcgggatc ccaggcgcag gtggccaggt
     5761 ccatgagcag cgtgcggctc gcgttggtga catccgtggc gtgcactcca ccgtggatgc
     5821 cccctgtcag gttccagagc agccaggtgt ccatcgtgcc gaaggcgagc tcacccgcct
     5881 cagcgcgtgc ccgagctcct tggacgttct ccaggatcca agcgatcttg gtcgccgaga
     5941 agtaggtcgc caagggaagg ccgaccttct ccttgaagcg atcgacatca cccgccgcga
     6001 tgcggtcgac tatcgactga gtgcgggtgt cctgccagac gatggcgttg tacaccggga
     6061 gtccggtcgt tcggtcccag acgaccgtgg tctcgcgctg gttggtgatt ccgacagcgg
     6121 cgatgtcgtg ccgggtgaga ttcgccttgc ccagcgcctg gccgatcact tcacgggtgt
     6181 tgtcccagat ctccaccggg tcgtgctcga cccacccagc ctgaggaaag tgctgtcggt
     6241 gttccagctg accgacggtg acgacggctc ccgcgtgatc gaagatcatc gctcgcgtgc
     6301 tcgtggtgcc ctgatcgagg gagagcacga atttcggcgt gtctgtggtc atgtcggctc
     6361 cttcgtcgca ccctcattgc accatatcaa cggcccggcg ggcccaccgc ggccaggtac
     6421 cgccactcgc cagcgtcgcg caggaaccga ctgttctcat gttgctcgcc gctgcctgcg
     6481 ggggatcggt agtgggcgac gaactcgacg gtgccttcgg tatcgagcaa cgcacccttc
     6541 gaccgcccga tgatgtcgag cctgtaccag cgcaggtcgg ggtcgagttg cagtgagcgc
     6601 ggtcgagtcg tcggatgcca tgacttcagc agatagtcgg catcgccgag cgcgaacgcc
     6661 gtgaagcggc tgcgcatgag tcgttcggcc gtgggcgccg tcgcacgacc atcgatgatg
     6721 ggtgcgcagc attcgctcaa gggcatgccg ctcagacagg ggcagcgcgt cacgcgagcc
     6781 gcctcacggg gtcagtgcgc tgacgtagcg gtgggcatcc cgggcgactg cgacccaggt
     6841 atcgagttct gttggctgcg cgaagctgac ccactcgcgc atggtgcgag ttcccatgct
     6901 caccacatct gcgtcgccag ccgcgacccg cgcctgtgcc tccggctcgg gaagcttggc
     6961 caccattcgg ccttcgcgat tgatgaacac gaagatggag tcatcaacgc tgagcccgcg
     7021 tccgttgaac atgcggccgg cctgtacgcc cgggactccg acgaaacgct cggcaacctg
     7081 atccagaagg gactcgccgc gttcgcgagc caactggcga tctgatgggc cggtcggcgc
     7141 tgccatttgt ccctccaagc gagcggtgtg tgctgacccg ggaagtttag agcctgtcgc
     7201 gagcgtgacg ctcgagcggt tcggcgtgca cggcgtgccg tgccgctcgc tctaggctgg
     7261 atgccgtgtc caaggtactg agcagtcttc ccgtcggcga gcgcgtcggc atcgccttct
     7321 ccggaggtct cgatacctcc tgcgccgtcg cctggatgcg cgagaagggt gcggtgccct
     7381 gcacctacac ggcggacatc ggccagtacg acgagccgaa catcggcgaa gtgccggcgc
     7441 gtgcaaagga gtacggtgcc gagatcgccc gcctcgtcga tgggcgcgcg gcgctcgtgg
     7501 aggaggggct cgtcgccctt gcctgtggcg cattccacat ccgttccggc ggaaagactt
     7561 acttcaacac gactccgctg ggtcgcgtcg tcaccggcac cctgctcgtg cgcgcgatga
     7621 aggaggacga cgtcgacatc tggggcgacg gctccaccta caagggcaac gacattgagc
     7681 ggttctaccg ctacggactg ctggccaacc cgcgcctgcg catctacaag ccctggctgg
     7741 atgccgactt cgtcggcgaa ctcggcggcc gcacggagat gagcgagtgg ctcgttgcgc
     7801 gcggctttcc gtaccgcgac tccaccgaga aggcgtacag caccgacgcc aacatctggg
     7861 gtgccaccca cgaggccaag aacctcgagg aactgagcac cggcctcgac atcgtcgacc
     7921 cgatcatggg cgtggccagc tggcgcgatg acgtcgagat tgccaccgag gaggtgacgg
     7981 tgcgctacga ggcgggccgt cccgtcgcga tcaacggcgt cgagttcacc gacccggttg
     8041 cactcgtgct ggaggccaac gcgatcggtg gacgccacgg tcttggcgca tccgatcaga
     8101 tcgagaaccg catcatcgag gccaagagtc gcgggattta cgaggccccc ggtatggcgt
     8161 tgctgcacat cacgtatgag cgcctgctca acgcgatcca caacgaggac acgattgcca
     8221 actaccacgc cgagggtcgc cggctcggtc gcctcatgta cgaaggtcgc tggctcgacc
     8281 cgcagtcgct catgcttcgc gagtcgctgc agcgctgggt cggttcggca gtgacgggcg
     8341 aagtcaccct gcgcctgcgc cgcggcgacg actacacgat cctcaacacg acgggaccga
     8401 acctcagcta ccaccccgag aagctgtcga tggagcgggt cggcgatgcc gcattcggtc
     8461 ccggcgatcg catcgggcag ctgacgatgc gcaacctcga catcgcggac tcgcgctcgc
     8521 gtctcgagca gtatgccgcg atcggcctca tcggcgggcc gacggccgag ctcgtcggcg
     8581 aactcgaggt gggtgccgcg aaccagatca ccggcggcgc ggatgacacg gcgctcgacc
     8641 gcgccaccga cgccgccaac gaggcttccg ccttcgagct cggcaccgac tgagtcagcg
     8701 ccggaccacc gccgggcccg tggcggctac cacgtggagt cgccccggat gatcacgtcc
     8761 gaaccgccgt cgatgaagac gatctgcccg cacaggtggg cgttctcctc actcgtgagc
     8821 caggcgagga ggtaggcgac cgatcgcggt tcgaagatgc cgttgagggg catcggaacc
     8881 atcgtcgtga tctgctcgcg ctgctcaggg gtggcgatga ggtctgccgt catcggggtc
     8941 tccacgactc ccggtgcgac gccgttgagg gggatcgacg cgcccgccca gtcggacgtc
     9001 gcggcgtggc ggcggatcca gagcgctaga gcgcgtttcg tggtgccgta gatcaggctt
     9061 ccgagctgcg ggccgcccgc ctcgagttcg gctccacggg cgagggcagc cttctcgtct
     9121 cccgcgagga gtgcggtgag gagggcgtca tccggcggga agagtgaagc catggaggcg
     9181 gtcgcgacgg cgcggggcgc cggggagttg accaacaggg ggcgcaggcc ctcgagggtg
     9241 gccacagtgc cgaagtagtt gacggcgacg gtgatggcgg tcggggtggc cagtcccgcg
     9301 ttggcgatga cggcgtcgat cgtgccgccg ctgagtgcag tgaccttctc gacgagggcg
     9361 gcccggccct cggcagtgga gagatccacc gtgacgtcgg cgtcgcggat gtcgactccg
     9421 atcaccgtgt ggcctcgctc aagcaggagt tctgtggttg ccttgccgat tccgctcgcg
     9481 gcaccggtga cgacgtaggt acgggacatg gcttcgacct tccgatcagg ggcgggcttc
     9541 tgcccgtcgg taccatgctc gtccggtcgt gcgcggccgg ttagggcatt tagtcccgcc
     9601 cctagcggga ctgcgagatc ggccgcagat gcacgcccag ctggccgtac caatcgcgga
     9661 cggcctgggc ctgggtctcg tcgcaggtgt acccgtagcc gatgctgacg tcccagtact
     9721 cgtagctgct cacgtagacg cggtggtcga gtgtccagcc cgagaggtag gtgagcgcga
     9781 agcagtccgc catggtttcg ggatctccgc cgaacgcctc gagagcggtg tcggtggcat
     9841 ccgggttggt catctggagc acgtgggcga actcgtggta ggcgatcccg gttcgtagcc
     9901 agtccgtcat gtacggctga cctgcgtctg ccgcgtcgaa gtgcacggcg aacggatcgt
     9961 cgtacacgac gcaggcgagg acgtcggctt cgcacagcgt gtcggcatca tcgatcaccg
    10021 tggtgacgtg accaccgccg aggtcatcga tcactgcctc gtaggtggtg ccgctcgtgt
    10081 tcgagcccgc ttgcgctgac gcctcggaga cgagatcctt cagcgtggcc tcctccgatt
    10141 ggatgcgacg ggtatcagct tcgacgctgc cggcgctcca gcggtgcgac caggccagac
    10201 gggcgatgct ctcgtagctg tcgagcggca ccagggagct catgaggctg ccgtcgagca
    10261 gactcgccgt ctcgagcagt tgcgccatcg ccgccccgaa cgtctccttc ttctcgatga
    10321 gctcgcgctg ctggtcgagt tcgcggttct gctcggcgat tcgggagttg gcggagagca
    10381 gctgcacgac caagaagatc acggcgccga tcgtgagcgc caagccgatg cccaggagta
    10441 tcagggctag acgcttcggt cgagcagcgg gggccttctc ctcggtgggc gatacctcga
    10501 cgaggccctc ctcgacagtc gggactgcag ttgtctcgtc cgacacggac tcctcctcgc
    10561 gttcgtgaga gtatatcgag tccgcgatcg accgggaatc aggtgggcgc ggatcggcga
    10621 cgctggccga cgacgacacc ggtgatcacg agaagagtgc ccacggcctg tcgaataccg
    10681 aaactctcac ccgcgagcag cgttcccaga agtacaccgg tgacggggtt gagaagtccc
    10741 acgagtccca cagtgcctgc tggcagatgg cgcagtcccg cgaaccacgc gatgaaagca
    10801 agcgccgtcg cgatgaccgc gacgtagacg tagccgagca cggcgccggg ttcaccggcc
    10861 gggggagcgc cttccagaat gaccgcgaag ggaagcacca cgagcccgcc gccgatgagc
    10921 tgccacgacg tcagtgcgac gaggggcacc tcggctcccc atctcttggt gagaatgaag
    10981 ccgatcgatg agagcaccat cgctgccagt gaggctatga ctccccaccc gtcaacggtg
    11041 agaccgccct cgaagagcag cacgccgact ccgaggaggc ccactgcggc accgatgacg
    11101 ccgaaccgcg tggggcgttc tcccaggagc ggccaagcca gcaggagcag cgccgctgcc
    11161 gaggtcgcca tgattgtcga cgctatgccc gacggcagtc gttgcgagac cacgtagatg
    11221 agcacgaaga atgcaccgac gttgagcgtg ccgagcacga gggaacgcca ccaccacgaa
    11281 ccccgaggaa gacggcgggc gagcaggagc agaatcaggc cggcggggag tgctcgaagg
    11341 agggcgcccc acagaggcgc gtccggcgga aggaagtgac gtgtgacgaa gtagttggag
    11401 ccccacgcga tcggggcgat cgcggtgacg agaatccagc gccatttacc ttccatggaa
    11461 gttatactag cttccatgga agacagtcgt ggcgacatcg gagatcgggt gtcccgcatc
    11521 cagagcgaat ggcgtcggga gcgacctgat gtcgacgttc agccgcaggg gatcatcggg
    11581 cgagtacatc ggctcgcagc gtggctcacc gatgagctga cggtggtcta cggacgctac
    11641 ggactggggg agggggaatt cgacgtgctc gccgcgctga ggcgcgccgg gcatcctttc
    11701 gagcgggctc cgggggaact cgccgaccac acgatggtga ccacaggggc catgacgaag
    11761 cgcatcgacc gtctcgtcgc tcgcgggctg gttgagcggc gtgaggtgga gcaggacggc
    11821 cgtcgccgca tcgtcgcgct cacgctggcc ggccgcgaac tcattgacgc cgccttcacc
    11881 gagcacatgc gcaacgaacg acgactcgtc gacgaactcg ccgccgatga ccaggccgca
    11941 ctcgaggcga tcctcacgcg ctggctcgcc gctggaccga cgcgctaggg gagcggggtg
    12001 aggtcgggct cgacgaatga cgtccgggcc tcgtccgagg cgagtcgacc ggcgctgtag
    12061 tgatcgtcga gccgatccgg acgcagcaac gcgggtcgat gatcagcgaa gtcgctgctg
    12121 gtctcgaacg gctccgttcg ggcgtcattg atgagacgca tccacgcgat cgtccga
//
LOCUS       DAOSOU010000044        12013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5275_length_12013_cov_5.901405, whole genome shotgun sequence.
ACCESSION   DAOSOU010000044 DAOSOU010000000
VERSION     DAOSOU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5275_length_12013_cov_5.901405"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLW15_03875"
     CDS             complement(<1..1134)
                     /locus_tag="PLW15_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT33573.1"
                     /translation="MHLLSVFSLRNRALIALVTIVVGLFGGVALTSLKQELIPSLSLP
                     QIFIITSYPGASPAVVDKDVSTPIEAAIQTVAGLDSTTATSSANVSQVTASFDYGTNI
                     ATAEQKVQLAINRLQNLPDGADTQVLTFNFSDLPVIQLAVTSDLDPLELTAKLNASTI
                     VELKKINDVSDASLLGSTTQRVVITPDPEELFARGLSNQSIRDALDDNGVLLPAGQIT
                     ENDLTLTVQAGARVTTADEVAALPLLGGNSLDLTTIADVATVEVVDDPVTGISRVNGE
                     PSVTIAITKTPAGNTVAVSRAVMAAIPDLEAALGSNTKLTPVFDQAPFIEESINSLAT
                     EGLLGLAFAVVVILLFLFSFRSTIVAAISIPVSVLITFIGMLAA"
     gene            complement(1272..2687)
                     /locus_tag="PLW15_03880"
     CDS             complement(1272..2687)
                     /locus_tag="PLW15_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT33574.1"
                     /translation="MGSLSARVLASLLDGWRDAGVAYAALADRIRLLILDGRIAPSTR
                     LPAERELAAQLGVSRTTVTAAYAELRDAGYLSSVRGSGSLAQLPIRSPLLPDPVRGDM
                     LDFSKAALPAIPQVLSAAQLAAQQLPQYIGESGFDPVGLPVLREALAARYTARGLPTT
                     ADQIMVTVGAQHAIALIARTIMARGDRALVENPSYPHAIEALKVAGARLVPVSVTSDE
                     GWDDVGLEQGIQRTSPSLGYLMPDNHNPTGRTMPTEQRVRVIELAARQGTTLIADETM
                     GELGLDGLPEPVPFAVHGPAILIGSVGKTVWGGLRIGWIRADRTIIQRLARARMSGDL
                     GTPLVEQLIVTNLLTDFDAILEARRATLRTGRDRLVRMLGESLPDWQVPHAAGGLTVW
                     ANLGAPVSSQLALAARNEGLVIAAGPRFGVDGAFERFIRIPFSYPAEETARAVAALAA
                     AWANVGRHPVAESANDFAGVV"
     gene            2749..3366
                     /locus_tag="PLW15_03885"
     CDS             2749..3366
                     /locus_tag="PLW15_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33575.1"
                     /translation="MVLTRRIGQLVVGLFLYGFGIALMVRGAIGVPPWDVLSQGISLR
                     TGLPFGLVTNIIGAAVLLLWIPIRQKPGIGTVANVLLIGPSAQFGLWVLPVLTDLVPR
                     ILVFALGLAVVALATGLYIGARMGPGPRDGLMTGIHRRYGWKLWIARSTIEVSVLIIG
                     WILGGNVGIGTLAFALLIGPMVNVTIPWLMVPAVVRDPELEEVPT"
     gene            complement(3588..4343)
                     /locus_tag="PLW15_03890"
     CDS             complement(3588..4343)
                     /locus_tag="PLW15_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT33576.1"
                     /translation="MDWSIQDIARLAGTTSRTLRHYGDVGLLPASRVGDNGYRYYDEL
                     ALVRLQRILMLRDLGLGIPAIADVLAGQTDGAEALAVHLRWLHQEKDRLDRQIASVEM
                     TMLTLEGGEQLMAENMFDGFDHTEYREEVEERWGAAAYADGDRWWRSKSAAEKAEWQQ
                     RQKQLAADWSAAAAAGLAPDGAEAQALATRHADWLAGIPGTPQVNGRPAKQYLVGLGE
                     MYVADDRFGANYGGVAGASFVRDALAVYAERNL"
     assembly_gap    4384..4483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4484..5104
                     /locus_tag="PLW15_03895"
     CDS             <4484..5104
                     /locus_tag="PLW15_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33577.1"
                     /translation="LGIDHVQLSWDNFIDVFKSFRGSARVDVSIQVPRDIALTFGVVS
                     ASALITGISADASISTVNGDVVVDSVTGDIQLNSVSGELSVLGHNGSIFAHTISGDIT
                     AAGEITKFTGDTVSGDVFLDIAGMPDQVRVNTVAGSVTTRLASDVAAKYSINTVSGRL
                     QLDDSEIRGVRGAYTGKYGTLEKHWLDFKANTVSGNISVLHAAVTV"
     gene            5101..5727
                     /locus_tag="PLW15_03900"
     CDS             5101..5727
                     /locus_tag="PLW15_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT33578.1"
                     /translation="MTPPVFAHGHLRLYLLSLLAESDHPMHGYELIQALGDRFGGTYV
                     PSAGTIYPRLAKLEDEGMVTKVADGRKTVYEITDAGRAELAARESELDSIESDVTDSV
                     RRLADEVRTSVTDAMRTLRADLAAAARDARAGANEFNKSYTSGSPTTTTSGSQLQEAE
                     SALASFRASVRTDLRTQQTKGGISDETIAVLTQGLEQVRAAVKASLGR"
     gene            complement(5820..6458)
                     /locus_tag="PLW15_03905"
     CDS             complement(5820..6458)
                     /locus_tag="PLW15_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33579.1"
                     /translation="MASTDSRRRRWPWIVAGVVVLLLVPVAVLGVPILLHQNQGASGQ
                     VDATEPWPLTVTGNGADGRTRTFTVSGEDGAAVDTSALTVGQHLVVRGSGFDGARGIY
                     VAICVIPSDPSVKPGPCIGGVPEQTPTAVDAGTVQYAPSNWINDDWAWKLFGARSYDD
                     IAKGTFTAYVEVGDPKGKDFDCRVDACGIFTRNDHTALSDRVQDLYVPVAFG"
     gene            6555..8594
                     /locus_tag="PLW15_03910"
     CDS             6555..8594
                     /locus_tag="PLW15_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOT33580.1"
                     /translation="MIGDPLPSEKLEGQLLPKHLALPIFASDPLSSVAYAPQELLMIL
                     LIGGLAFLSFAPWVAMAVVILMIVVVLSYRQLVRAYPSGGGDYEVASKNLGEKAGLIV
                     ASALLVDYVMTVAVSVASGVDNIISAIPALNPFRVEIAVGFIILLAAVNLRGVRESSK
                     AFAVPTYLFIGSIGVMIVVGLVRTIGGDAPIAESAGLDVRAESLTQAAIILLLLRSFA
                     SGCAALTGVEAIANGVPAFRKPKIQNAQKTLVAMGLIAITLFSGLVILALVSGVHYAE
                     DACHLIGFDNCETVPQRSLIAQIASATFGNNSIPFFIIQTATAAVLLLAANTAFNGFP
                     LLGSILAADHYAPKSLSTRGDRLIFSNGVIALAIVAGVILVIFRADVTSLIQLYIIGV
                     FVSFTLGQTGMVRHWIRLLKEGATNRGQIIRSLATNAVGATMTASVLIVVTITKFTHG
                     AWVVFVIMPILFLLMLGVNRYYRDVDKEIEADPTTRFGAKGDHAIVLVGRMQKPVLKA
                     LDYAIAARHGSIEAVHVSIDDAATEELERDWVEQNIKVPLNVLESPYRDISAPLAKYI
                     KSRRQTHGSEVVTVYTPQYIVGHWWENILHNHKARRIRQKLSLVHGVVIALVPWLLDS
                     STLIYGRRSRPLPGEDRRGEPRRPVVRKPMPPADPTAAAAVKTVVRPKTQAEKKK"
     gene            8596..8994
                     /locus_tag="PLW15_03915"
     CDS             8596..8994
                     /locus_tag="PLW15_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOT33581.1"
                     /translation="MPWRDLLAVLVGGAVGTALRLGIDTVLPHGDADFPVSTLAINTL
                     GAFVLGFLVARVWPAATSWLRAGLGAGLLGSFTTFSALAVSLVSLTAAGFGMLALAYL
                     VATLVLGLAAAALGLRLGSPRVTDVDLVDE"
     gene            8991..9362
                     /gene="crcB"
                     /locus_tag="PLW15_03920"
     CDS             8991..9362
                     /gene="crcB"
                     /locus_tag="PLW15_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOT33582.1"
                     /translation="MIAAAVIVAGALGAVVRYLVSRWLSSAEGAYRNLPLAVLIVNVV
                     GSAIGGAVLGLATAGGVDAGIRLVLLTGVCGGLTTFSTFSVETVQYVTEGKWRIALAS
                     VAANLVLGIGAAAAAYALTVG"
     gene            complement(9359..10465)
                     /locus_tag="PLW15_03925"
     CDS             complement(9359..10465)
                     /locus_tag="PLW15_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOT33583.1"
                     /translation="MTSAGTDTSVVVIGAGQAGLSVSYYLQRLGLDAGNDFVVLDRGP
                     GTGGAWQFRWEALRIGSAHKINDLPGLDALGLSFETADRHAPAKDVVADYYRRYEDHY
                     GLQVVRPADVVSVENFGMDLMVNFTLDDEEKEVSTVTVVNATGTWGAPFIPWYPGLKS
                     FEGRHVHTNDYKSAEDYRDQSVIVVGGGTSAIGFLLELEGIASDTTWVSRRPIDFLED
                     GDLNLEARIEAVRMQDEAARAGRALPSIVSGTGVPRTRRIQAGIERGVLTPRPIFDSI
                     EPHGVRWKDGAFKKADAIIWSTGFRPELRHLAPLKLREKEGGVVVAQGASWKDPRIFF
                     AGYGPQASTIGANRAGRMIARQVVATLSKIQASR"
     gene            10621..10836
                     /locus_tag="PLW15_03930"
     CDS             10621..10836
                     /locus_tag="PLW15_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HOT33584.1"
                     /translation="MGRGRQKAKHTKVARDLKYFSPDTNYNALAQELSGAPSDPRLAE
                     DLEKWPEYVAGSYEEDEFDTDDESKTA"
     assembly_gap    10839..10938
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10939..11999)
                     /gene="purM"
                     /locus_tag="PLW15_03935"
     CDS             complement(<10939..11999)
                     /gene="purM"
                     /locus_tag="PLW15_03935"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT33585.1"
                     /translation="MTEDSSRSYRDAGVDTAAGDLAVELMKSAVSRTHGPQVLGGVGG
                     FAGMFDVSFLKAFDRPLLASSTDGVGTKVAIAQAIDKHDTIGQDLVGMVVDDIVVVGA
                     RPMFMTDYIACGRVIPQRIADIVAGIARACEATGTALVGGETAEHPGLLGPDDYDVAG
                     AAVGAVEAADLLGPDRVADGDIVIGMASSGLHSNGFSLVRHILAQRGVGYTDASAELG
                     GTVGEILLEPTRLYTTPLLDVLAEHPGAVHALSHVTGGGIAANLARVLPVGSWVELDR
                     GSWSPPAVFRVLSDMAGSTLVSAEGTWNLGVGMIAVVTPAAADGVIASLERSGIPSWK
                     LGEVSMSARDLSGFESEAKG"
BASE COUNT         1853 a         4074 c         3934 g         1952 t
ORIGIN      
        1 ggccgcgagc atcccgatga aggtgatgag caccgagacg gggatcgaga tcgcagcgac
       61 gatcgtcgaa cggaatgaga acaggaacag caagatcacg acgaccgcga aggcgaggcc
      121 gagcaggccc tcggtcgcga gcgagttgat cgactcctcg atgaacgggg cctggtcgaa
      181 gaccggggtg agtttggtgt tcgagccgag agcagcctcg aggtcgggga tcgcggccat
      241 cacggcgcgc gacacggcga cggtgttgcc agccggggtc ttggtgatgg cgatcgtgac
      301 cgacggctcg ccgttgacgc gggagatgcc ggtgaccggg tcgtcgacga cctcgacggt
      361 cgcgacatcc gcgatggtgg tcaggtcgag cgagttgccg cccaacagcg gcagggcggc
      421 cacctcgtcg gcggtcgtga cccgggcgcc ggcctgcacc gtgagcgtca ggtcgttctc
      481 ggtgatctga ccggcgggca gcagcacgcc gttgtcgtcg agggcgtcac ggatcgactg
      541 gttcgacagg ccgcgtgcga acagctcctc ggggtcgggg gtgatgacca cgcgctgggt
      601 ggtcgagccc agcaggctcg cgtcactgac gtcgttgatc ttcttgagct cgacgatcgt
      661 cgacgcgttg agcttggcgg tgagctcgag ggggtccagg tcgctggtga cggcgagctg
      721 gatgaccggc aggtcggaga agttgaaggt gagcacctgg gtgtctgcgc catccggaag
      781 gttctgcaga cggttgatcg ccagctgcac cttctgctcc gcggtggcga tgttggtgcc
      841 gtagtcgaac gaggccgtga cctgggagac gttcgcgctc gaggtcgcgg tggtcgagtc
      901 gagcccggcg acagtctgaa tcgccgcctc gatgggcgtc gagacatcct tgtcgacaac
      961 cgcagggctg gcgcccgggt agctcgtgat gatgaagatc tgcggcagcg agaggctggg
     1021 gatcagctcc tgcttgagcg aggtgagcgc gacaccgcca aagagaccga caacgatcgt
     1081 gacgagagcg atgagcgcgc ggttgcgcag gctgaagact gagagaagat gcacacccta
     1141 ttgtggcccg actgcacggg cggcggtgta ctccctcagg agtaacccgc tggttgagta
     1201 gccgcgcagc ggcgtatcga aacccgtgag cgaaccggtt tcgatacggc ctgcggccta
     1261 ctcaaccggc cctagacgac ccccgcgaag tcgttcgccg attctgccac cggatgccgc
     1321 cccacattcg cccacgcggc cgccagcgca gccactgctc gcgccgtctc ctccgccggg
     1381 tacgagaagg ggatgcggat gaagcgctcg aacgcgccat cgacgccgaa gcgtgggcca
     1441 gccgcaatca cgagtccctc gtttcgggcc gcgagagcga gctgcgaact gaccggggca
     1501 ccgaggttcg cccagaccgt gagcccaccg gcggcatggg gtacctgcca gtcgggcagg
     1561 ctctcgccga gcatccgcac cagacggtcg cggccggtgc gcagggtcgc gcgacgggcc
     1621 tcgaggatgg cgtcgaagtc ggtgagcagg ttggtgacga tgagctgctc gacgagcggc
     1681 gtgcccaggt cgcccgacat tcgcgcgcga gcgaggcgct ggatgatcgt gcggtcggcg
     1741 cggatccacc cgatgcgcag accgccccac accgtcttgc ccacggaacc gatcaggatc
     1801 gccggcccgt gcacggcgaa cggcaccggc tcgggcaggc cgtcgaggcc gagctcaccc
     1861 atcgtctcgt cggcgatcag cgtcgtgccc tggcgtgccg cgagttcgat cacccgcacc
     1921 cgctgctcgg tcggcatcgt gcggccggtc gggttgtggt tgtcgggcat gaggtagccg
     1981 agcgacgggc tggtgcgctg gatgccctgc tcgagcccca cgtcatccca cccctcatcg
     2041 ctcgtgacac tcacgggcac cagccgcgcc cccgcgacct tcagcgcctc gatcgcgtgc
     2101 gggtaactcg gattctcgac gagcgcccga tcaccgcgcg ccatgatcgt gcgggcgatc
     2161 agcgcgatgg cgtgctgggc tccgacagtg accatgatct ggtcggccgt ggtggggagc
     2221 cctcgagcgg tgtaccgggc ggcgagcgcc tcgcgcagca ccggcaggcc caccggatcg
     2281 aagccggact cgccgatgta ctgcggcaac tgctgcgccg cgagctgagc ggccgagagc
     2341 acctgcggga tcgcgggcag ggcggccttg ctgaagtcca gcatgtcgcc ccgcaccggg
     2401 tcgggcagaa ggggcgagcg gatcggaagc tgcgcgaggc tgccggaacc gcgcacgctc
     2461 gacaggtagc cggcatcccg cagctcggcg taggcggcgg tcaccgtggt gcgactcaca
     2521 ccaagctgcg cggcgagctc acgctcggcg ggcaggcggg tcgacggcgc gatgcgcccg
     2581 tcgaggatca gcagacggat gcggtcggcg agcgccgcgt acgcgacccc ggcatcacgc
     2641 cagccatcca atagcgatgc caatactcgc gcagacagag aacccatggt gccactttag
     2701 gcagattggc ctctagatac caagccaatt cggggattgg attgctccat ggttcttaca
     2761 cgacgaattg gccagctggt ggtcggactg ttcctctacg ggttcggcat cgctctgatg
     2821 gtgcgcggcg ccatcggagt accgccgtgg gatgtgctct cgcagggcat ctcgctgcgc
     2881 accggtctgc cgttcgggct cgtgaccaac atcatcggtg cggccgtgct gctgctgtgg
     2941 attccgattc gccagaagcc cggcatcgga accgtggcca acgtgctgct gatcggcccg
     3001 agcgcacagt tcggactgtg ggtgctgccg gtgctcaccg accttgtgcc gcgcatcctc
     3061 gtgttcgcgc tcgggctggc cgtcgtcgcc ctcgcgacgg gcctctacat cggtgcgcgc
     3121 atggggcccg gcccgcgcga tggcctcatg acgggcatcc accgccgcta cggctggaag
     3181 ctgtggatcg cgcgcagcac catcgaggtg agcgtgctca tcatcggctg gattctcggc
     3241 ggcaacgtcg gcatcggaac tctcgccttc gcgctgctca tcggcccgat ggtcaacgtg
     3301 acgatcccgt ggctgatggt gccggccgtt gtgcgcgacc ccgaactcga ggaggtgccg
     3361 acatgatcgc attctggatg ctcggtgcgc tcgccgcggt cgcgatcgtt gccaccgtgc
     3421 gcagcgtcgt caccgacggc taccggaggg tgccgacccg ctaccggtga tcgagcggtc
     3481 cggtgatcga gctcgtcgag atccggtgat cgagctcgtt gagatccggt gatcgagctt
     3541 gtcgagatcc ggccgagatc tcgaccccac tcgatcaccg gctcccctta caggttgcgc
     3601 tccgcgtaca ccgcaagcgc atcgcgcacg aagctcgcgc ccgcgacgcc gccgtagttc
     3661 gcgccgaagc ggtcatccgc gacatacatc tcgccgagtc cgacgaggta ctgcttcgcc
     3721 ggccgaccgt tcacctgggg cgttcccggg atgcccgcca gccagtcagc gtgtcgggtc
     3781 gccagggcct gtgcctcggc tccgtccggc gcaagcccgg ccgctgccgc cgcggaccag
     3841 tccgccgcca gctgcttctg cctctgctgc cattcggcct tctcggccgc cgacttggag
     3901 cgccaccagc gatcgccgtc ggcgtacgcg gcggcgcccc aacgctcctc gacctcctcg
     3961 cggtactcgg tgtggtcgaa tccatcgaac atgttctctg ccatgagttg ttcacctcct
     4021 tccagtgtga gcatcgtcat ctcgaccgac gcgatctgcc gatcgagccg gtccttctcc
     4081 tgatggagcc agcgcagatg cacagcgagc gcctcggcgc cgtcggtctg gcccgcgagc
     4141 acgtcggcga tcgccggtat gcccagcccc aggtcgcgca gcatcaggat gcgctgcagc
     4201 cgcacgagcg cgagctcgtc gtagtagcgg tacccgttgt cgccgacgcg ggacgcgggc
     4261 agcaacccga cgtcgccgta gtgccgcagc gtgcgggacg tggtgcccgc cagccgcgcg
     4321 atgtcctgaa tcgaccagtc catgtgtctg cctccttctg caaccacgct agaggttgac
     4381 gcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcggca tcgaccacgt
     4501 gcagctgagc tgggacaact tcatcgacgt gttcaagagc ttccgcggca gcgcccgcgt
     4561 cgacgtgagc atccaggtgc cccgcgacat cgcgctcacc ttcggcgtcg tctcggccag
     4621 cgccctcatc accggcatct cagcggatgc ctcgatcagc acggtcaacg gcgacgtcgt
     4681 cgtcgacagc gtgaccggcg acatccagct caactccgtc agcggtgagc tgtcggtgct
     4741 cggacacaac ggaagcatct tcgcccacac catcagcggc gacatcaccg cggccggcga
     4801 gatcaccaag ttcaccggcg acaccgtctc cggcgacgtc ttcctcgaca ttgcggggat
     4861 gcccgaccag gtacgggtca acaccgtcgc cggctcggtc accactcgcc tcgcttcgga
     4921 cgtcgcggcg aagtactcca tcaacaccgt gagcgggcgt ctgcagctcg acgattccga
     4981 gattcgcggc gtgcgcggcg cctacacggg caagtacggc acgctcgaga agcactggct
     5041 cgacttcaag gccaacacgg tctcgggcaa catctcggtg ctgcacgccg cggtgacggt
     5101 atgaccccgc ccgtcttcgc ccacggccac ctgcgcctct acctgctgag cctgctcgcc
     5161 gagtccgatc acccgatgca cggctacgag ctgatccagg cgctcggcga ccgcttcggc
     5221 ggcacctacg tgccgagcgc cggcaccatc tacccgcgtc tcgccaagct cgaggacgag
     5281 ggaatggtca ccaaggtcgc cgacggccgc aagaccgtct acgagatcac ggatgccgga
     5341 cgcgccgagc ttgccgcccg cgagtccgaa ctcgacagca tcgagagcga cgtcaccgac
     5401 tcggtgcgac ggctcgccga cgaggtgcgc acctctgtca ctgacgcgat gcgcaccctg
     5461 cgcgccgacc tcgccgctgc cgcgcgcgac gctcgggccg gagcgaacga gttcaacaag
     5521 agctacacct cgggctcccc cacgacgacg accagcggca gtcagctgca ggaggcggag
     5581 tccgcgctcg cgagcttccg ggcgtccgtg cgcaccgacc tgcgcaccca acagaccaag
     5641 ggtggaatca gcgacgagac gatcgccgtg ctcacccagg gtctggagca ggtgcgggca
     5701 gccgtgaagg cgtcgctggg tcgctagata cgccgggtgc ggggctgcgc ttcaactcgc
     5761 gcgaggtgtc gctcgatcag ctgcggtgat cgagcctgtc gagatctcga ctcgctcgat
     5821 cagccgaacg cgaccggcac gtagaggtcc tgcacccggt cgctgagcgc ggtgtggtcg
     5881 ttcctcgtga agatgccgca ggcatccact cggcagtcga agtccttgcc cttcggatcg
     5941 ccgacctcga cgtaggcggt gaaggtgccc ttggcgatgt cgtcgtagct gcgagcgccg
     6001 aacagcttcc aggcccagtc gtcgttgatc cagttgctcg gcgcgtactg cacggtgccg
     6061 gcatccactg cggtgggcgt ctgctcggga accccgccga tgcacggccc cggcttcacg
     6121 ctggggtccg acgggatgac gcagatcgcc acatagatgc ctcgcgcccc gtcgaacccg
     6181 cttcccctca cgacgaggtg ctggccgacg gtgagcgcgg aggtgtcgac tgctgcgccg
     6241 tcctcgccgc tgaccgtgaa ggtgcgggtg cgaccgtctg cgccgttacc cgtgacagtg
     6301 agcggccagg gctcggtggc atccacctgc cctgaggctc cctggttctg gtgcagcaga
     6361 atcggcacgc cgagtaccgc cacgggaacg agcaggagca cgacgacacc cgcgacgatc
     6421 cacggccacc gcctgcgccg ggaatcagtt gaggccatgg gggaattcta agcggcctga
     6481 tcgagttgac tgcctttctc ataggagtaa gctcgcccct cgtgacagac gaagcccggt
     6541 ctcctaaacg gtggttgatc ggagacccac tgccgtccga gaaattggaa ggccagctac
     6601 tccccaagca cctcgctctc cccattttcg cgagcgaccc gctctcctcc gtggcctacg
     6661 cgccgcagga actgctcatg atcctgctca tcggcgggct cgccttcctc tccttcgccc
     6721 cgtgggtggc gatggccgtc gtgatcctca tgatcgtcgt cgtgctgtcg taccgtcagc
     6781 tggtgcgggc ctacccgagt ggtggcggcg actacgaggt cgcgtccaag aacctgggcg
     6841 agaaggccgg cctgatcgtc gcatccgcgc tgctcgtcga ctacgtgatg accgtcgcag
     6901 tgtcggtggc ctccggtgtc gacaacatca tctccgcgat ccccgcgctc aacccgttcc
     6961 gcgtcgagat cgccgtcggc ttcatcattc tgctcgccgc ggtcaacctg cgcggcgttc
     7021 gcgagtcgag caaggcgttc gccgtgccga cctacctgtt catcggcagc atcggcgtca
     7081 tgatcgtcgt cggtctggtg cgcacgatcg gtggcgatgc cccgatcgcc gagagcgccg
     7141 ggctcgacgt gagggcggag tccctcaccc aggcggcgat catcctgctg ctgctgcgat
     7201 cattcgcgag cggatgcgcc gcactcaccg gtgtcgaggc gatcgccaac ggcgtgccgg
     7261 ccttccgcaa gccgaagatc cagaatgccc agaagacgct cgtggcgatg ggcctcatcg
     7321 ccatcacgct gttctcggga ctggtgatcc tcgctctcgt ctccggcgtg cactacgcgg
     7381 aggacgcctg ccacctcatc ggcttcgaca actgcgagac ggtgccgcag cgcagcctga
     7441 tcgcgcagat cgcgtcggcg acgttcggca acaactcgat cccgttcttc atcatccaga
     7501 ccgccaccgc cgccgtgctg ctgctggccg ccaacacggc gttcaacggg ttcccactgc
     7561 tcggttcgat cctcgccgcc gaccactacg cgcccaagtc cctgtcgacc cgcggtgacc
     7621 gcctgatctt ctccaacggc gtgatcgcac tcgcgatcgt ggccggcgtg atcctcgtga
     7681 tcttccgggc ggatgtcacg agcctcatcc agctctacat catcggcgtc ttcgtgtcgt
     7741 tcacgctcgg acagaccggc atggtgcggc actggatcag gctgctgaag gagggcgcga
     7801 cgaatcgcgg ccagatcatc cggagtctgg cgaccaacgc tgtcggcgcg acgatgaccg
     7861 cgagcgtgct gatcgtggtg acgatcacca agttcaccca cggtgcgtgg gtggtcttcg
     7921 tgatcatgcc gatcctcttc ctgctgatgc tcggcgtgaa ccgctactac cgcgacgtgg
     7981 acaaggagat cgaggccgac cccaccacgc gattcggcgc gaagggcgac cacgccatcg
     8041 tgctcgtcgg tcgcatgcag aagcccgtac tcaaggctct cgactacgcg atcgccgcgc
     8101 gccacggctc aatcgaggcc gtgcacgtgt cgatcgacga tgcggctacc gaggaactcg
     8161 agcgggactg ggtcgagcag aacatcaagg ttccgctcaa cgtgctcgag tcgccgtacc
     8221 gcgacatcag cgctccgttg gcgaagtaca tcaagtcgcg gcgccagacg catggctccg
     8281 aggtcgtcac ggtctacaca ccccagtaca tcgtcggcca ctggtgggag aacatcctgc
     8341 acaaccacaa ggcccgtcgc atccgtcaga agctctcgct ggtgcacggc gtggtcatcg
     8401 ccctcgtgcc gtggctgctc gactcctcga ccctcatcta cggccgccgc tcccgcccgc
     8461 ttccgggtga ggatcggcgt ggtgagccgc gacgtcccgt ggtgcgcaag cccatgccgc
     8521 cggccgaccc gacggccgct gccgcggtga agaccgtcgt gcgcccgaag acccaggccg
     8581 agaagaagaa gtagcgtgcc ctggcgtgac ctgctggccg tgctcgtggg cggcgcggtg
     8641 ggcacggccc tgaggctcgg catcgacacc gtgctcccgc acggcgatgc ggacttcccg
     8701 gtctcgacgc tcgcgatcaa caccctcggt gcattcgtgc tggggttctt ggtcgcacgg
     8761 gtctggcccg ctgccacgag ctggctgcgg gccggcctcg gcgccggact cctcgggtcg
     8821 ttcacgacct tctccgcgct ggccgtgtcg ctcgtctcgc tcaccgccgc cgggttcggg
     8881 atgctcgccc tcgcctacct cgtagcgacc ctggtgctcg gcctcgccgc cgccgcgctc
     8941 gggctgcgac tgggctcgcc gcgcgtcacc gacgtggacc tggtggacga gtgatcgccg
     9001 ccgccgtcat cgtcgcgggt gccctcgggg cggtcgtgcg gtatctggta tcccgctggt
     9061 tgagtagcgc cgaaggcgcg tatcgaaacc tcccgctcgc tgtcctcatc gtcaacgtgg
     9121 tgggctcggc gatcgggggc gcggtgctgg gactcgcgac cgccgggggc gtcgacgcgg
     9181 gcatccgtct ggtgctgctg accggcgtgt gcgggggcct gacgaccttc agcacgttca
     9241 gcgtcgagac cgtgcagtac gtcaccgagg gcaagtggcg catcgccctc gcgagcgtcg
     9301 cggccaacct cgtgctcggc atcggggcgg ccgcagcggc ctacgcgctc accgtcggct
     9361 agcgcgaggc ctggatcttg ctcagggtcg cgacgacctg acgggcgatc atgcggcccg
     9421 cgcggttggc cccgatcgtg gatgcctgcg gcccgtaccc tgcgaagaag atgcgggggt
     9481 ccttccacga tgcgccctgc gcaaccacga cgccgccctc cttctcccgg agcttgagcg
     9541 gtgcgagatg tctgagctcc gggcggaatc ccgtggacca gatgatcgca tccgccttct
     9601 tgaaggctcc gtctttccag cgcacgccgt gcggttcgat cgagtcgaag atcggccgcg
     9661 gtgtgagcac cccacgctcg atgccggcct ggatgcgccg cgtgcgcggc acccccgtgc
     9721 ccgacacgat gctcggcagg gctcgaccgg ctcgcgccgc ctcgtcctgc atgcgcacag
     9781 cctcgatgcg ggcctcgagg ttcaggtcgc cgtcctccag gaaatcgatc gggcgacggc
     9841 tcacccaggt ggtgtcgctg gcgatgccct ccagttcgag aaggaagccg atcgcgctcg
     9901 taccgccacc cacgacgatc acgctctggt cgcgatagtc ctcggcgctc ttgtagtcgt
     9961 tggtgtgaac gtggcggccc tcgaagctct tcaggccggg gtaccagggg atgaagggcg
    10021 ctccccaggt gcccgtcgcg ttgacgacag tgacggtcga cacctccttc tcctcgtcgt
    10081 cgagggtgaa gttgaccatc agatccatgc cgaagttctc cacgctgacc acgtcggcgg
    10141 ggcgcacgac ctgcaggccg tagtgatcct cgtaccggcg gtagtagtcg gcgacgacgt
    10201 ccttggccgg agcgtgacgg tcggcggtct cgaagctcag gccgagcgca tccagacccg
    10261 gaagatcgtt gatcttgtgg gccgaaccga tgcgcagcgc ctcccaccgg aactgccagg
    10321 caccgcccgt tcccggcccg cgatcgagca cgacgaagtc gtttcccgcg tcgagaccga
    10381 gcctctgcag gtagtacgag acggacagcc cggcctgccc cgcgccgatc accactactg
    10441 aggtatcggt gcctgctgac gtcacccgac aacgttaccc gtcagcggga tgagcgtgga
    10501 gaaccgcccc ggctggctgg gtgccgcgtg ctaaactgag cgctagtttt tccaccatcc
    10561 ctattcgcac aatccctgcg tggtagcagc gcatcgggac tgatccagag ggggtctcgc
    10621 atggggcgtg gccgtcagaa ggcaaagcac accaaggtgg cgcgggacct gaagtacttc
    10681 agtcctgaca ccaactacaa cgcgcttgcc caagagctct ccggagcgcc cagcgaccct
    10741 cgcctcgccg aggacctcga gaagtggccg gagtacgttg ccggcagcta cgaggaagac
    10801 gagttcgaca ccgacgacga gtcgaagacc gcctgaggnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnncc cttggcttcg ctctcgaagc cgctgaggtc gcgcgcgctc
    10981 atcgacactt cgccgagctt ccacgacggg atgccgctgc gctccaacga ggcaatgacc
    11041 ccgtcggccg ccgccggggt gaccaccgcg atcatcccca ccccgaggtt ccaggttccc
    11101 tcggcgctca cgagcgtgct gccggccatg tcgctgagca cccggaacac ggcggggggc
    11161 gaccagctgc cgcgatcgag ttcgacccac gaacccaccg ggagcacgcg cgcgaggttg
    11221 gccgcgatgc ctccgccggt cacgtggctg agcgcgtgca ccgcgccggg atgctccgcc
    11281 agcacgtcga gcagcggggt cgtgtagagc cgggtcggct cgagcaggat ctcgccgacg
    11341 gttccgccta gttctgccga ggcatccgtg tagccgacgc ctcgctgggc gaggatgtgg
    11401 cgcaccagcg agaacccgtt ggagtgcagg ccgctcgacg ccattccgat cacgatgtcg
    11461 ccgtcggcga cccggtcggg tccgagtagg tcggcagcct cgaccgcgcc cacggcagcg
    11521 ccggcgacgt cgtagtcatc aggaccgagt agccccgggt gctcggcggt ctcgccgccg
    11581 acgagcgccg ttccggtcgc ctcacaggcc cgcgcgatgc ccgcgacgat gtcggcgatg
    11641 cgctgcggga tgacgcgccc gcacgcgatg tagtcggtca tgaacatcgg ccgcgccccg
    11701 accaccacga tgtcgtcgac gaccatgccc accaggtcct gcccgatggt gtcgtgcttg
    11761 tcgatcgcct gggctatcgc gaccttcgtg ccgaccccgt cggtggagct cgccaagagc
    11821 gggcggtcga atgccttcag gaagctgacg tcgaacattc cggcgaagcc gccgacgcca
    11881 ccgagcacct gcggcccgtg cgtgcgcgag accgcggact tcatgagttc gacggccaga
    11941 tcgcctgccg cggtgtcgac tccggcgtca cggtagctgc gggaggagtc ctcggtcacc
    12001 cgaccagcgt acc
//
LOCUS       DAOSOU010000045        11749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5425_length_11749_cov_7.391910, whole genome shotgun sequence.
ACCESSION   DAOSOU010000045 DAOSOU010000000
VERSION     DAOSOU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_11749_cov_7.391910"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..335
                     /locus_tag="PLW15_03940"
     CDS             9..335
                     /locus_tag="PLW15_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT33586.1"
                     /translation="MTALEVPTEPVAASRTVAGAPRDGATELTIFEGCEIGIWEMTPG
                     TATDVEVDEVFVVLAGAATITFDDGTVVELKPGSFCRLHTGQRTRWDVTATLRKMYVV
                     AAEGAS"
     gene            332..979
                     /locus_tag="PLW15_03945"
     CDS             332..979
                     /locus_tag="PLW15_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT33587.1"
                     /translation="MTELVPIPSPGIPLFYGDPGNPVVIVLHDWYGRLPGLDSFAEAL
                     ASRGLRVAVPDLYQGVCTTDAATAESLMTSLDLSTALAEVDDAIAQAQDTGDERVAIV
                     GFSMGGWLGLLHAQGGSVDAVVAYYASLSEAEHGVIPCPVLFQWAEVDEWETGDGPEE
                     FVARLKDHGTPVTEHRYIGTEHGFANASIPSKINAQAAALAFARTAVFLEKHLVG"
     gene            complement(1016..1534)
                     /locus_tag="PLW15_03950"
     CDS             complement(1016..1534)
                     /locus_tag="PLW15_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT33588.1"
                     /translation="MAITPDTKNWTWVLEKPCPECAFDASTFEATDVADMIRANAAAW
                     PAVLERDDVRERPDESTWSKLEYAAHVRDVFRIYLYRLGLMLAEDDPLFPNWDQDATA
                     VEERYNEQSPEVVSAELVADAAALADAFDTVADWSRPGRRSDGVSFTIETFAKYFIHD
                     PIHHLWDVTKAG"
     gene            1579..1878
                     /locus_tag="PLW15_03955"
     CDS             1579..1878
                     /locus_tag="PLW15_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HOT33589.1"
                     /translation="MPTFSDDIVTAVLRHMNEDHPEDNILIVTAFGHKDAWAATMIGL
                     DGDGGSWAYLLGDDERVVTVPWSGPITERPEIRREIVKVYDAACAKLGVEPRPHD"
     gene            1942..2589
                     /locus_tag="PLW15_03960"
     CDS             1942..2589
                     /locus_tag="PLW15_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HOT33590.1"
                     /translation="MSVIPFSQALRERTRSSHGDSAGATFMSDLMTGQGSRDDYIALV
                     AQHYYIYEALEAAAVRFADDAVASPFISPQLTRLPALELDLDYLVGPDWRDAHPPVPA
                     TTRYVDRITEVADEGWAGGFVAHHYTRYLGDLSGGQAIAKIMQRQFGFETNGVAFYIF
                     DQIASPKEFKETYRDQLDSVEWDAAERERVIDEVLLAYTFNTDLFIDLAAAKAAA"
     gene            complement(2711..4006)
                     /locus_tag="PLW15_03965"
     CDS             complement(2711..4006)
                     /locus_tag="PLW15_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT33591.1"
                     /translation="MSEVSLSPEQQAVYDRIETTRDNIFVTGRAGTGKSTLLNYLSWN
                     TSKQLVISAPTGVAALNVGGQTIHSLFRLPIGVIADHDIDQSAELKKLLNTIDTLVID
                     EVSMVNADLLDAIDRSLRQARQRKNEAFGGVQVVLFGDPYQLAPVPGEGDERAYFADR
                     YDSMWFFDAKVWDETDLTIYELSTIHRQHEDEFKRMLNAVRHGYVTAEIAGRLNEVGA
                     RPAPTDGAITLATTNGTVTRINATELARLPGRVLTARAEINGEFGGRAYPADEALELK
                     VGARVMFLRNDVGGESQRWVNGSVGTVLKIDTTVWVEIDGERHEVKPAVWEKFKYNYS
                     PLTKSLRKDVVAEFTQFPLRLAWAVTIHKSQGKTYDRAIVDLGQRSFAPGQTYVALSR
                     ISELEGLYLTRPLRPSDIIVDENVVRFMSRTTAIPAIRA"
     gene            4137..5099
                     /locus_tag="PLW15_03970"
     CDS             4137..5099
                     /locus_tag="PLW15_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33592.1"
                     /translation="MRREIITWSIVVAAVIGGFVGTVLILNASLYSASGFVRSYLDAL
                     ARHDAPGALELAGPSVAGDASTQLLSRDAMGELGDIRLISDAAQPDGTHTVVYAYSAG
                     TVVGQSSFAVRPTGSLLGLFTTWSFDTTPLGVVQLTVLHEDEFTANGVALTTPRQNES
                     APYLVFTPGLYEFDHQSTFLTAEPVRIAVTEPSSAVPAELDVQANDAFAEEVSREVNE
                     FLDACATQQVLLPTGCPFGQTITNRVVTSPEWSIIDYPKVTIIPGGEPSQWRMSQASA
                     TAHLIVDVKSLFDGTVSSLDDDVPFTVRATIAMLPEDDLIITVD"
     gene            complement(5086..7071)
                     /locus_tag="PLW15_03975"
     CDS             complement(5086..7071)
                     /locus_tag="PLW15_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOT33593.1"
                     /translation="MPRLAKIVAPATLVLAALVAVFAALAFGGGADPLLLVDPGEVVR
                     YGLPVSKLLVNLAGAGSVGALILVLFALDSAKPEFGRALDVAAASAAVWTVASGATGF
                     FTFLLVYNQPIRIDDTFGQVLGLFLTSTELGQAWLATTLFAAVVTVLCFAVRNVTMLV
                     FVAVIAVAGLVPMALQGHRGGTANHDAATSALFLHVLFAAIWLGGLLTIAIIRPLLDR
                     GRLEVVLRRYSTVALVSFVVVAVSGYVSAAIRVENLPNLLSPYGILVLVKVAALLALG
                     VFGAAQRRYFIRRMTRSTDSAAARIFWVVAAAELAFMGIASGVAAALARTPTPVPQVP
                     ASELTDPTPAEMLTGSPLPPPVSLQNLFTMWNIDLIWLLVCGFGILFYVWGVIRLRRR
                     GDSWPWHRTVLWVAGLLALAYITNGGVNVYERYLFSSHMLAHMVLGMVIPVLLVPGAP
                     ITLALRTIEKRSDGSRGPREWIMLLVHSKYFGVLSNPLVAAGLFTGSLWLFYYTPLFD
                     WATTNHIGHEWMVIHFLLTGYLFVFSLIGIDPAPNRAPFPIRLVTLFATMAFHAFFGL
                     ALVTGTGLLLVDWYGAMGWGTKAILDQQAGGAIAWSVGEIPNAIIAIAIVVLWSRSDD
                     REQKRYDRKADRDGDAELEEYNRMLAERAASKPVSRQ"
     gene            complement(7177..7464)
                     /locus_tag="PLW15_03980"
     CDS             complement(7177..7464)
                     /locus_tag="PLW15_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT33594.1"
                     /translation="MADKSLNRTEFVAAVAAESGQSQAAVNGVVDAIFAVLATSVGEG
                     TKVSIPGWLAVERTHRSARTGRNPQTGEAIQIKASYGVKVSAGSKLKAAAK"
     gene            complement(7523..7828)
                     /gene="rpsN"
                     /locus_tag="PLW15_03985"
     CDS             complement(7523..7828)
                     /gene="rpsN"
                     /locus_tag="PLW15_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOT33595.1"
                     /translation="MAKKSKIAKNEQRKVIVARYSAKRLELKKALVDPNGTDESREAA
                     RLGLQKLPRNASPVRVRGRDSIDGRPRGFLSQFGVSRVRFRDMAHRGELPGITKSSW"
     gene            complement(7832..7999)
                     /gene="rpmG"
                     /locus_tag="PLW15_03990"
     CDS             complement(7832..7999)
                     /gene="rpmG"
                     /locus_tag="PLW15_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT33596.1"
                     /translation="MAKQQDIRPIIKLRSTAGTGYTYVTKKNRRNNPDRLVLKKYDPV
                     IRQHVEFREER"
     gene            complement(7999..8235)
                     /gene="rpmB"
                     /locus_tag="PLW15_03995"
     CDS             complement(7999..8235)
                     /gene="rpmB"
                     /locus_tag="PLW15_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT33597.1"
                     /translation="MAAVCQVTGAVPGFGHNISHSHRRTKRRFDPNIQKKTYFVPSLR
                     RSVTLTLSAKGIKVIDARGIESVVKDLLARGEKI"
     gene            8427..9185
                     /locus_tag="PLW15_04000"
     CDS             8427..9185
                     /locus_tag="PLW15_04000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT33598.1"
                     /translation="MPPVLQDIVITPTVDGASLHLKGLLGIPDSPGPWPAVVVVHEAF
                     GIEAEMRKQVTHLASLGYLALMPDLYTEGGFRKCIGATARALRSGTGRAWADIESARL
                     WLTGRDDFTGALGIIGFCMGGGFALLAAASGYDSEETAYQAAAVNYGILPADLDVIEH
                     SCPVVASYGSKDRAVKDAPARLEALYTRLGVAHDVKEYPGAAHAFLNEKPTGPLLIRP
                     FLKISGIAPDPVAAVDAWKRIDAFFGEHLVPARP"
     gene            9182..>10164
                     /locus_tag="PLW15_04005"
     CDS             9182..>10164
                     /locus_tag="PLW15_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT33599.1"
                     /translation="MRIGYAVGYWKQGPPKGALKAIRTAERLGYESVWTAEAYGSDAF
                     TPLAWWGSRTTRIKLGTGIAQLSARTPTAAAMAALTLDHLSGGRVILGLGASGPQVVE
                     GWYGAPYPKPLARSREYIDVVRQVLARQSEVTSAGPHYPLPYTGPDATGLGKPLRSTV
                     HPLRADLPIYLAAEGPKNVALAAEIADGWLPFLFSPKADAEARAQLEEGFALRSPERS
                     PRENFEIALQLAVIPHPDVEKAADMVRPTIALYVGGMGAKGANYHRNVIDRLGYSDAA
                     DRIQELYLAGKKAEAIAAVPTKLVEEIALIGPPEKIRDELAAWEESVVTTML"
     assembly_gap    10165..10264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10265..10590)
                     /locus_tag="PLW15_04010"
     CDS             complement(<10265..10590)
                     /locus_tag="PLW15_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT33600.1"
                     /translation="MKRNTWQREAVREALGTREGFVSAQSLHSALRDGGSSIGLATVY
                     RALADLAVEGEADSLQQEGESLYRACTPGTHHHHLICRNCGLTVEIEADAVEQWARQV
                     ASEHGFT"
     gene            complement(10587..11426)
                     /locus_tag="PLW15_04015"
     CDS             complement(10587..11426)
                     /locus_tag="PLW15_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOT33601.1"
                     /translation="MDLATYFELLQLVQASVLAGAVLGVVGGLIGVFVMQRDMAFAVH
                     GISELSFAGAAAALLLGVNVVAGSILGSLIAAAIIGLLGARARDRNSIIGVLMPFGLG
                     LGILFLALYPGRSGNKFGLLTGQIVSVDFEQLGLLVGISVVVLAALLVIWRPLSFDSL
                     DTEVAVAKGVPSRALALVFMVLLGLTVAVSVQVIGALLVLALLVTPAASALRVSASPV
                     LVPVLAMIFGLVSAVGGILIALGGSLPISPYITSISFLIYVVCRLIEWRRQRSQARVK
                     VAA"
     gene            complement(11426..>11749)
                     /locus_tag="PLW15_04020"
     CDS             complement(11426..>11749)
                     /locus_tag="PLW15_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33602.1"
                     /translation="VLLLCDEPLASLDLNRQAEVAGLIDERRREHDTAVVFVTHDINP
                     VLGMVDRILYLAEGHFTVGTPDEVLRSEVLTALYGTPVEVLRSGGRIFVAGAPDHAHH
                     QGEAL"
BASE COUNT         1951 a         3951 c         3910 g         1837 t
ORIGIN      
        1 ccctcgatct gaccgccctc gaggtgccga ccgagcccgt cgcggcatcc cgcactgtcg
       61 ccggcgcgcc gcgcgatggt gccaccgagc tgaccatctt cgagggatgc gagatcggga
      121 tctgggagat gacccccggc acggccaccg acgtcgaggt ggacgaggtc ttcgtcgtgc
      181 tcgcgggcgc cgcgacgatc acgttcgacg acggcactgt cgtcgaactg aagcccgggt
      241 cgttctgccg tctgcatacc gggcagcgca ctcgctggga cgtgaccgcg acgctgcgca
      301 agatgtacgt cgtggcggcg gagggcgcgt catgaccgag ctcgtgccga taccgtcgcc
      361 gggcatcccg ctgttctacg gcgacccggg caacccggtg gtcatcgtgc tgcacgactg
      421 gtacggtcgc ctgcccggcc tcgactcctt cgccgaggcg ctggcctccc gcgggctgcg
      481 ggtcgcggtg cccgacctgt accagggcgt ctgcacaacg gatgccgcga ccgccgaatc
      541 gctcatgacc agcctcgacc tctcaacggc cctcgccgag gtcgacgacg cgattgcgca
      601 ggctcaggac accggcgacg agcgggtcgc gatcgtcgga ttctcgatgg gcggctggtt
      661 ggggctgctc cacgcgcagg ggggttcggt cgacgccgtg gtggcgtact acgcgagtct
      721 cagcgaggcg gagcacggcg tcatcccgtg cccggtgctc ttccagtggg ccgaggtcga
      781 cgagtgggag acgggcgacg gcccggagga gttcgtcgcg cgcctcaagg atcacggcac
      841 gcccgtcacc gagcaccgct acatcggcac cgagcacggg ttcgccaacg cgagcatccc
      901 gtcgaagatc aacgcgcagg ccgccgcgct ggcgttcgcg cgcacggccg tgttcctgga
      961 gaagcacctc gtcgggtgac gcggtatcgg tttcgatacg cgccttcggc gctactcaac
     1021 cggccttcgt cacgtcccac aggtggtgga tcgggtcgtg gatgaagtac ttcgcgaacg
     1081 tctcgatcgt gaacgagacc ccgtcgctgc ggcggccggg gcggctccag tccgcgaccg
     1141 tgtcgaatgc gtcggcgagc gccgcggcat ccgccaccag ttctgccgag accacctcgg
     1201 gggactgctc gttgtagcgc tcctcgaccg cggtggcgtc ctggtcccag ttggggaaca
     1261 gcgggtcatc ttccgcgagc atgagcccca gccgatacag gtagatgcgg aagacatcgc
     1321 gcacatgagc cgcgtactcg agcttcgacc aggtgctctc gtcgggacgt tcgcgcacat
     1381 cgtcgcgctc gagcacagcg ggccacgccg ccgcgttggc gcggatcatg tcggccacgt
     1441 cggtcgcctc gaaggtgctc gcgtcgaatg cgcactcggg gcagggcttc tcgagcaccc
     1501 acgtccagtt cttggtgtca ggggttatcg ccatgacacc atcgtgccct gtgccgccga
     1561 ccggctaccg tagatatcgt gccaaccttc tccgacgaca tcgttaccgc cgtgctgcgc
     1621 cacatgaacg aggaccaccc cgaggacaac atcctcatcg tcaccgcctt cggtcacaag
     1681 gatgcctggg ccgcaaccat gatcggcctc gacggcgacg gcggctcctg ggcctacctc
     1741 ctcggcgacg acgagcgagt ggtcaccgtg ccctggtcgg ggccgatcac cgaacggccc
     1801 gagatccggc gcgagatcgt caaggtctac gacgcggcgt gcgcgaagct cggcgtggag
     1861 cctcgcccgc acgactagtc gccccagcgg cgctcggcta aactttaggc ttaccttacc
     1921 taccgttctc aggagctccg gatgagcgtc atccccttct cgcaagccct tcgcgagcgc
     1981 acccgctcga gtcacggcga cagcgccggc gccaccttca tgagcgacct catgaccggt
     2041 cagggttcgc gcgacgacta catcgcgctc gtcgcccagc actactacat ctacgaggcc
     2101 ctcgaggctg ccgccgtccg gttcgcggat gacgcggtcg catccccctt catcagcccg
     2161 caactgacgc ggctgcccgc cttggagctc gacctcgact acctcgtggg acccgactgg
     2221 cgcgacgccc accctcccgt gcccgcgacc acccgctacg tcgaccgcat caccgaggtc
     2281 gccgacgagg gctgggccgg cggattcgtc gcccaccact acacgcgcta cctcggggac
     2341 ctctccggcg gccaggcgat cgcgaagatc atgcagcgcc agttcggctt cgagaccaac
     2401 ggtgtcgcgt tctacatctt cgaccagatc gcctccccga aggagttcaa ggagacctac
     2461 cgcgaccagc tcgactcggt cgagtgggat gccgcggagc gcgagcgggt catcgacgag
     2521 gtgctactcg cctacacgtt caacacggac ctgttcatcg acctcgccgc agcgaaggct
     2581 gctgcctagg cgggttgagt aggccgaagg ccgtatcgaa accccaaccc aggcgggttg
     2641 agtaggccga aggccgtatc gaaaccccac acctgaggtt tcgatacgcg cttcgcgcta
     2701 ctcaacctgc ctaagcgcga atcgccggga tcgcagtcgt gcgggacatg aaacgcacca
     2761 cgttctcgtc gacgatgatg tcgctggggc gcagcggacg cgtcaggtag agaccttcca
     2821 gctcgctgat gcggctgagg gccacgtagg tctggccggg cgcgaatgag cgctggccga
     2881 ggtcgacgat cgcgcggtcg taggtcttgc cctgcgactt gtggatggtg accgcccacg
     2941 ccaaccgcag cgggaactgc gtgaactcgg ccacgacatc cttgcgcagc gacttggtca
     3001 gcggcgagta gttgtatttg aacttctccc agaccgcggg cttcacctcg tggcgctcgc
     3061 catcgatctc cacccacacg gtcgtgtcga tcttgagtac cgtgcccacg ctgccgttca
     3121 cccagcgctg cgactcaccg ccgacgtcgt tgcgcaggaa catcacccgc gcgcccacct
     3181 tgagttcgag cgcctcgtcg gccgggtacg cgcgcccacc gaactcgccg ttgatctcgg
     3241 cccgcgcggt gagcacccga ccgggcaggc gggcgagctc ggtggcgttg atgcgcgtga
     3301 cggtgccgtt cgtggtggcc agggtgatcg cgccatccgt cggggcgggt ctggcgccca
     3361 cctcgttgag gcgacccgcg atctcggcgg tcacgtatcc gtgccgcacc gcgttgagca
     3421 tgcgcttgaa ctcgtcctcg tgctggcgat ggatggtcga cagctcgtag atcgtgaggt
     3481 cggtctcgtc ccagaccttc gcgtcgaaga accacatcga gtcgtaccgg tcggcgaagt
     3541 aggcgcgctc gtcgccctcc cccggcactg gggcgagctg gtacgggtcg ccgaacagca
     3601 ccacctgcac cccgccgaat gcctcgttct tgcgctggcg ggcctgccgc agcgaccggt
     3661 cgatcgcatc gagcaggtcg gcgttgacca tcgacacctc gtcgatcacg agggtgtcga
     3721 tcgtgttgag gagcttcttg agttccgcgc tctggtcgat gtcgtggtcg gcaatgacgc
     3781 cgatcggcag acggaacagc gagtggatgg tctgcccgcc cacgttgaga gcggccacgc
     3841 cggtcggagc gctgatcacg agctgcttcg aggtgttcca cgacaggtag ttgaggaggg
     3901 tcgacttgcc ggtgccggca cggccggtga cgaagatgtt gtcgcgagtg gtctcgatgc
     3961 ggtcgtagac ggcctgctgc tcaggggaca ggctcacctc gctcacggat tcactgtacc
     4021 tgcggcgatg gtcgggtctc ggcaagctcg accaccggtg cacagcccgg cctcggtgca
     4081 cagggcgggc gggtctcggc aagctcgacc accggtgtgc aacttaggat gatggaatgc
     4141 gccgggagat catcacgtgg tcgatcgtgg tggccgccgt catcggcgga ttcgtgggga
     4201 ccgtgctgat cctcaacgcc tccctctaca gcgcgagcgg ttttgtgcgc agctacctcg
     4261 acgcgctggc gcgacacgac gcaccggggg cgcttgagct cgccggcccg agcgtcgcgg
     4321 gcgacgcgtc gacccagttg ctctcgcgcg acgcgatggg ggagctgggc gacatccggc
     4381 tgatcagtga cgcggcgcag cccgacggca ctcacacggt cgtctacgcc tacagcgccg
     4441 gcacggtcgt gggccagtcg tcgttcgccg tgcgtccgac cggatcactt ctcggcctgt
     4501 tcaccacgtg gtcgttcgac accacgccgc tgggggtcgt gcagctcacc gtgctccacg
     4561 aggacgagtt cacggccaac ggggtcgcgc tcaccacacc tcggcagaac gagtcggcgc
     4621 cgtacctcgt cttcacgccc ggcctctacg agttcgatca ccagtcgacg ttcctcaccg
     4681 ccgagcccgt gcgcatcgcc gtgaccgagc cgagctcagc ggtgccggcc gagctcgacg
     4741 tgcaggccaa cgacgccttc gccgaggagg tgtcgcgcga ggtcaacgag ttccttgacg
     4801 cgtgcgccac ccagcaggtg ctgctgccga cggggtgccc gttcggccag acgatcacca
     4861 accgggtggt cacgagcccg gagtggtcga tcatcgacta cccgaaggtg acgatcatcc
     4921 ccggcggcga gccgagtcag tggcggatgt cgcaggcctc cgccacggcc cacctcatcg
     4981 tcgacgtcaa gagcctcttc gacggcacag tgtcaagcct cgacgacgac gttccgttca
     5041 ccgtgcgcgc aaccatcgcg atgctgcccg aggacgacct catcatcact gtcgactgac
     5101 gggcttgctg gccgcgcgct ccgcgagcat gcggttgtac tcctccagct cggcatcgcc
     5161 gtcgcgatcc gccttgcggt cgtagcgctt ctgctcgcga tcgtcactgc gagaccagag
     5221 cacgacgatc gcgatcgcga tgatcgcgtt ggggatctcg ccgacgctcc aggcgatcgc
     5281 gccgccggcc tgctggtcga gaatcgcctt cgtgccccag cccatcgcgc cgtaccagtc
     5341 cacgaggagc aaccccgtgc cggtgacgag cgcgagcccg aagaacgcgt ggaaggccat
     5401 cgtcgcaaac agggtgacga gtcggatcgg gaacggcgca cggttgggcg ccgggtcgat
     5461 gccgatgagc gagaacacga acagatagcc ggtcagcagg aagtggatga ccatccactc
     5521 gtgtccgatg tgattggtcg tcgcccagtc gaacagcggc gtgtagtaga acagccagag
     5581 cgagccggtg aacagcccag ccgcgaccag cggattgctc agcaccccga agtacttcga
     5641 gtgcacgagc agcatgatcc actcccgcgg cccccggctg ccatcgctgc gcttctcgat
     5701 cgtgcgcagg gcgagcgtga tcggtgcccc gggcaccagc agcacgggaa tcaccatgcc
     5761 cagcaccatg tgcgcgagca tgtgcgacga gaacaggtag cgctcgtaca cgttcacgcc
     5821 gccgttggtg atgtaggcga gcgccagcag ccccgcgacc cagagcacgg tgcggtgcca
     5881 gggccacgag tcgccccgcc gacgcagacg gatgactccc cacacgtaga acagaattcc
     5941 gaagccgcac accagcagcc agatcaggtc gatgttccac atcgtgaaca ggttctgcag
     6001 ggacaccggc ggcggcagcg ggcttcccgt gagcatctcg gcgggtgtgg ggtccgtcag
     6061 ctcggaggcg ggcacctgtg gcacgggagt cggcgttcgt gccagtgccg cggccacgcc
     6121 cgacgcgatg cccatgaacg cgagctcggc ggcggccacc acccagaaga tgcgagctgc
     6181 ggcggagtcg gttgagcgcg tcatccggcg gatgaaatac cggcgctggg cggcaccgaa
     6241 cacgccgagt gccagcaggg ccgccacctt cacgagcacg aggatgccgt acgggctcag
     6301 caggttcggc aggttctcga cgcggatcgc cgcactcacg tagccgctca ccgcaaccac
     6361 cacgaagctg acaagcgcga cggtggagta ccggcgcagc accacctcga ggcggccccg
     6421 gtcgaggagc ggtctgatga tcgcgatggt gagcaggcca ccgagccaga tggcggcgaa
     6481 cagcacgtgc aggaagaggg cgctggtggc ggcgtcgtgg ttggcggttc cgccgcggtg
     6541 gccttgaagc gccatgggta cgaggcctgc cacggcgatc acggccacga agaccagcat
     6601 ggtgacgttg cgcacggcga agcagagcac cgtgacgacc gccgcgaaca gggtggtggc
     6661 cagccacgcc tggccgagct cggtgctggt gaggaagaga ccgagcacct ggccgaaggt
     6721 gtcgtcgatt ctgatcggct ggttgtagac gagcaggaac gtgaagaacc cggtcgcgcc
     6781 cgatgcgaca gtccagacgg cggcgctcgc ggcggccacg tcgagggccc tgccgaactc
     6841 gggcttggcc gagtcgaggg cgaacagcac gagaatcagc gcgccgacgc tgcccgcgcc
     6901 ggccagattg accagcagct tggagaccgg caggccgtag cgcacgacct cgccgggatc
     6961 gaccagcagc agcgggtcgg ctccaccacc gaaggcgagc gccgcgaaga ctgcgacgag
     7021 tgccgcgagc acgagagtcg cgggggcgac gatcttcgct aagcggggca ccctatgagc
     7081 ctattcgtcg cgggctgggc atccacccga tcagcccgca ggaacgacga agggcgacga
     7141 ccattacggt cgccgcccta tcgaacgtgc gctgaattac ttagcggcag ccttgagctt
     7201 gcttccggcc gaaaccttca cgccgtagct cgccttgatc tggattgcct cgccggtctg
     7261 cgggttgcga ccggtgcgtg ccgagcggtg cgtacgctcg actgcgagcc accccgggat
     7321 cgacaccttg gtgccttcac cgaccgacgt ggccagaacg gcgaagatag cgtcgacgac
     7381 gccgttgacg gcagcctggc tctggccaga ctcggcggca acagccgcga cgaactcagt
     7441 acggtttagt gacttgtcag ccatgggatg tcctcctcgg acctcatgca gttatgggtg
     7501 gtgcctgtgt agcgcctgaa atctaccagc ttgacttggt aattccgggc aactcacccc
     7561 ggtgagccat atcgcggaag cgaacacggg aaacaccgaa ctgcgagagg aaaccacgcg
     7621 gacggccatc gatcgagtcg cggccgcgca cgcgcacggg cgaagcgttg cggggaagct
     7681 tctgcaggcc gaggcgcgcg gcctcgcggg actcgtcggt accgttcggg tcgacgagcg
     7741 ccttcttcag ctccaggcgc ttggcggagt agcgggccac gatgaccttg cgctgctcgt
     7801 tcttggcaat cttgctcttc ttggccatgt gttagcgctc ctcgcggaat tcgacgtgct
     7861 ggcggatcac cgggtcgtac ttcttgagca cgaggcgatc ggggttgttg cgacggttct
     7921 tcttcgtcac gtaggtgtag cccgttcctg cggtcgaacg aagcttgatg atggggcgga
     7981 tgtcctgctg cttggccatt agatcttctc cccacgggca agaaggtcct tgaccacgga
     8041 ctcgatgcca cgagcgtcga tcaccttgat gcccttggcg gacagcgtga gcgtgacgct
     8101 acggcgcagc gaaggcacga agtaggtctt cttctggatg ttcgggtcga aacgacgctt
     8161 ggtgcgtcgg tgcgagtgcg agatgttgtg cccaaagccg ggaacggctc cggtcacctg
     8221 gcagactgct gccatgggtg tactcctgtt ctggttaccg tgactcatcc gcttccggat
     8281 ggatcaccca agatcacttg tcggcacgcc ttgatcccct aacgtttctg gggaggcata
     8341 cgtgcgggcg gaaatgccca acctgtagat actacgtggg cggccccggc acgtgcaacc
     8401 tggcgcgtgt ctgcgagaat cgccccatgc ctcccgtgtt gcaggacatc gtgatcaccc
     8461 cgaccgtgga tggcgcgagc ctccacctca agggactgct gggcatcccc gactcacccg
     8521 gcccgtggcc ggcggtcgtc gtggtgcacg aggcgttcgg catcgaggcg gagatgcgca
     8581 agcaggtcac ccatctggcg agtctgggat acctcgcgct catgcccgac ctgtacacgg
     8641 agggcggctt ccgcaagtgc atcggcgcca cggcacgcgc cctgcgcagc ggcaccgggc
     8701 gtgcctgggc cgatatcgag tcggcgcgac tctggttgac cgggcgtgac gacttcaccg
     8761 gcgccctcgg catcatcggc ttctgcatgg gtggaggatt cgcgctgctg gccgcggcga
     8821 gtgggtatga cagcgaggag accgcctatc aggcggccgc cgtgaactac ggcatcctgc
     8881 ccgccgacct cgacgtgatc gagcactcct gccccgtggt cgcgagctac ggctcgaagg
     8941 accgcgccgt caaggatgcc cccgcccgcc tcgaggcgct gtacacgcgc ctgggcgtcg
     9001 cgcacgacgt gaaggagtac ccgggcgcag cgcacgcgtt cctcaacgag aagccgaccg
     9061 gcccgctcct gatccgcccc ttcctgaaga tctccggcat cgcccccgac ccggtggccg
     9121 ccgtcgacgc gtggaagcgc atcgacgcgt tcttcggcga gcatctggtg ccggcccgac
     9181 catgaggatc gggtatgccg tcggctactg gaagcagggg ccgccgaagg gcgcgctgaa
     9241 ggcgatccgc accgccgagc gtctcggcta cgagtcggtc tggacggccg aggcttacgg
     9301 ctccgacgcg ttcacaccgc tcgcctggtg gggttcgcgc acgacgcgca tcaagctcgg
     9361 caccggaatc gctcagctct cggcgcgcac tcccaccgcc gcggcgatgg cggcgctcac
     9421 cctggatcac ctgtcgggcg gccgggtgat actcgggctc ggggcatccg gaccccaggt
     9481 cgtcgagggc tggtacggag cgccctaccc gaaaccgctc gcgcgcagtc gcgaatacat
     9541 cgacgtcgtg cgccaggtgc tcgcccggca gagcgaagtc acgtcggccg gcccgcacta
     9601 cccgctgccc tacaccggcc cggatgccac gggcctcggc aaaccgctgc gctccaccgt
     9661 gcaccccctg cgcgccgacc tgccgatcta cctcgccgcc gagggaccca agaacgtcgc
     9721 cctcgccgcg gagatcgccg acggctggct gccattcctg ttctcgccga aggccgacgc
     9781 cgaggcgcgc gcccagctcg aggagggctt cgccctgcgc tcacccgagc ggtcgccgcg
     9841 ggagaacttc gagatcgcgt tgcagctcgc cgtgatcccg caccccgacg tggagaaggc
     9901 ggccgacatg gtgcggccga cgatcgcgct gtacgtcggc ggcatgggcg cgaagggcgc
     9961 gaactaccac cggaacgtga tcgaccgctt ggggtactcc gacgcggccg accgcatcca
    10021 ggagctctat cttgcgggca agaaggccga ggccatcgcc gccgtgccga cgaagctcgt
    10081 ggaggagatc gcgctcatcg gtccgccgga gaagatccgc gacgaactgg ccgcgtggga
    10141 ggagtcggtc gtcacgacca tgctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnngtgaac ccgtgctcgg aggcgacctg gcgcgcccac tgctcgacgg catccgcttc
    10321 gatctcgacg gtgagcccgc agttgcggca gatcaggtgg tggtgatgag tgcccggggt
    10381 gcaggcgcga tacaggctct cgccctcctg ctgcagtgag tcggcctcgc cctcaacggc
    10441 gaggtcggcg agcgcccgat agaccgtggc caggccaatg gatgacccgc cgtcacgcaa
    10501 cgccgagtgc aggctctgcg cgctcacgaa gccctcgcgc gtgccgaggg cctcgcgcac
    10561 ggcctcccgc tgccaggtgt tgcgcttcat gcagccacct taaccctcgc ctgggagcgc
    10621 tgtctgcgcc attcgatcag tcggcagacc acgtagatca ggaacgagat gctcgtgatg
    10681 tacgggctga tcggcagcga tccgccgagg gcgatcagga tgccgccgac cgctgagacg
    10741 agcccgaaga tcatcgcgag caccggcacg agcaccggtg atgcgctgac tcggagcgcg
    10801 gatgccgcgg gcgtgaccaa cagcgcgagc acgagcagcg cgccgatcac ctgcaccgag
    10861 acggccaccg tgagtcccag cagcaccatg aacacgaggg cgagcgcacg gctcggaaca
    10921 cccttggcaa cggcgacctc cgtgtcgagg ctgtcgaagc tcagcgggcg ccagatcacg
    10981 agcagggcgg cgagcacgac gacgctgatg ccgacgagca acccgagctg ttcgaaatcc
    11041 accgacacga tctgcccggt gagcaggccg aacttgttgc cgctgcggcc ggggtagagc
    11101 gcgaggaaca ggatgccgag ccccagcccg aagggcatga gcacgccgat gatggagttg
    11161 cggtcccggg cacgcgcgcc gagcagtccg atgatcgcgg ccgcgatgag ggacccgaga
    11221 atcgacccgg ccacgacgtt gacgccgagc agcaaggcag cagcggcgcc cgcgaaggag
    11281 agctcgctga tgccgtgcac cgcgaaggcc atgtcgcgct gcatcacgaa gacgccgatg
    11341 agacctccga cgacgccgag caccgctccg gcgagcacgg atgcctgcac cagctggagc
    11401 agttcgaagt aggtggcgag gtccatcaga gggcctcccc ctggtggtgg gcgtggtcag
    11461 gggcacccgc aacaaagatc ctgccgccac tgcgcagcac ctccaccggg gtgccgtaca
    11521 gcgcggtgag cacctcgctg cgcagcacct cgtcgggagt tcccacggtg aagtggccct
    11581 cggccaggta gaggatgcgg tcgaccatcc ccagcaccgg gttgatgtcg tgggtgacga
    11641 agaccactgc ggtgtcgtgc tcgcggcgcc gctcatcgat gagccccgcg acctccgcct
    11701 gacggttgag gtcgaggctg gccagtggct cgtcgcacag cagcagcac
//
LOCUS       DAOSOU010000046        11697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5468_length_11697_cov_6.880175, whole genome shotgun sequence.
ACCESSION   DAOSOU010000046 DAOSOU010000000
VERSION     DAOSOU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5468_length_11697_cov_6.880175"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1180)
                     /locus_tag="PLW15_04025"
     CDS             complement(17..1180)
                     /locus_tag="PLW15_04025"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT33603.1"
                     /translation="MNANLGPWAKSFPAASWGLSQEEFLSTSPRLSHFSTPVLSIDSA
                     AATSNVALMAGWMSERGFELAPHGKTTMAPKLWQQVLDAGAWGITLATPWQVQVARSV
                     GIKRVILANEFVDPVALGWLGAELADPDFEFLCWVDSIEAVDAMRAGLRRAARPVDVV
                     VELGGVRTGARSIREALAVAAAAEAAPELRLVGVGGYEGAMGGSRSAKDLAAVQQYLS
                     DLVELSAAIHWRQQPIVTAGGSAFFDLVAEALTPLVGAATVIVRSGAYQLHDAGFYRG
                     ITPLPGLRSAIHGWSRVLSRPEPELALLDGGKRDFPYDLDMPRSVHGPLSKMNDQHGF
                     LPLAPTADVSVGDVLRLDLSHPCTAMDKWRLVPVIDSADASDPEVIDLVETFF"
     gene            1276..2022
                     /locus_tag="PLW15_04030"
     CDS             1276..2022
                     /locus_tag="PLW15_04030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT33604.1"
                     /translation="MAEFDSLVAIVTGGASGIGAAVARRLAEGGATVAVFDLKPAEAD
                     GHFAVAVDVSDDASVVSAVAAVVETFGRIDIVVNNAGVGAQGDVTANDDSEWHRVWDI
                     NAVGMARVTRAALPHLRVSPSAAVVNVASIVATAGVPQRVLYSATKGAVLSMTRAMAA
                     DHVREGIRFNAVNPGTADTPWINALLATAEHPAAERAALEARQPHGRLVAPEEIADAV
                     AYLASPRSGSTTGTSIAVDGGMQELRLRPA"
     gene            2074..2940
                     /locus_tag="PLW15_04035"
     CDS             2074..2940
                     /locus_tag="PLW15_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT33605.1"
                     /translation="MRLVRIGAAGSESPGVLVDDDTVVDLSDVVGDYDENFFASGALA
                     NLGDVVADRVRAGRTEPVAGRRFGAPIARPHQILCIGLNYSDHAAETGQAVPTEPILF
                     TKSPNTLIGPDDDVLIPRGSTKTDWEVELGIVIGRHSSYLADEAEASASIAGYVLVND
                     VSERAWQMERNGQWSKGKSAPTFNPAGPYLVTPEEVGDVLNLRMTLDVDGRRRQDGST
                     ATMIFSPAFIVHYLSQFMALEPGDLINTGTPPGVGMGQKPPTYLRGGEVMTLSITRLG
                     TQTQRVIAASAS"
     gene            complement(2963..3457)
                     /locus_tag="PLW15_04040"
     CDS             complement(2963..3457)
                     /locus_tag="PLW15_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33606.1"
                     /translation="MEQYLWVLKSTERDLIRELEPERLALLDEDGLMELHKRVRRARN
                     KHSKNYRRGAASGVTEVGARGTAASKSDKSRARAYVFEEALSIVSGELARVAHDAAEA
                     LKDERLARARAGKSTGPESAGTQDGTVGAGRVRSHSKTTGGVKRDASSRAQGAKRQAK
                     RDGR"
     gene            complement(3491..4327)
                     /locus_tag="PLW15_04045"
     CDS             complement(3491..4327)
                     /locus_tag="PLW15_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT33607.1"
                     /translation="MIIDSHLHVWDPSRANYPWLDDGLGSVNRTISFDEIAPALAERG
                     IGGAVLVQASDNAEDTDVMLAVAARQPAVLGVVAWTPLDRPEQVALDVQRFEAIVVGV
                     RNLVHEREREWVLQPAVGDSLELLAAAGVPLDFPTADYAALADIATLGERHPSLTIVI
                     DHLGKPPIGRDEAAHVEWRALLAECAANPRTVAKVSGLYASVGPLDSWTVDAVRPFVE
                     DALDLFGADRLLYGGDWPISELAGGYARTWDAVTGILDSQPAEVRAAILGGTAARVYR
                     LG"
     gene            complement(4342..5316)
                     /locus_tag="PLW15_04050"
     CDS             complement(4342..5316)
                     /locus_tag="PLW15_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT33608.1"
                     /translation="MRSTTLGRAPALSEIGLGTSTFGNLYRETTDAETVDAVDVAWAG
                     GVRYFDTAPHYGLGLAERRLGAALAGRPRDEFVVSTKVGRALVPTPENAHQLDDDGFH
                     VPAEYRREWDFSRDGILRSLEQSLTRLGMDRVDVLYLHDPDHHWEQASTEGIATLIEL
                     RDQGVVRAIGAGMNQSAMLARFVRECDVDLVMLAGRFTLLDQGALDDLLPIALERGVG
                     VVAAGVYNSGLLGRAVVPDDAHYEYQAAPAELLARARAIAELCGAHGVTLPDAAVQFP
                     LRHPAVVSAVLGARNGEQMQGGLDRDSAPIPDALWSDLEAAGFIPSLA"
     gene            5434..6600
                     /locus_tag="PLW15_04055"
     CDS             5434..6600
                     /locus_tag="PLW15_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOT33609.1"
                     /translation="MMKITGYRCLRSFHDWGRPVGDVNGFIESGVTEVPIVILETDEG
                     VTGVATGSHHDLERLFPAIEGQDPRAVSALYDRMLARVFKTGHGGATYSGIGTLDTAL
                     WDIKAKLAGEPLWRLLGGGDRFVAGYASGLDIALDDDQLASFYRTMAERGFTAGKLKG
                     GRVVADDIRRLHIIADELRGNADHPALMLDANESWNLKQAVRHVTAVEAVHDLTWIEE
                     PLRRWDAAGHARLSRSIKAAVATGEKLTGLDQYLPLFEAGAVDVAQVGSPAGVTHFLR
                     VAYAAHSRDLPISPVGLTANASVAHAAAAVPNHLSAEVQDLGGPFGIDIDMEYADGGI
                     VLGDRPGAGLTIDEDAIAARGTAGGAWLVQGGPHVRPKRAGLRMVAEDPSSLPG"
     gene            6629..7399
                     /locus_tag="PLW15_04060"
     CDS             6629..7399
                     /locus_tag="PLW15_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOT33610.1"
                     /translation="MTDHAADVPVRNSVLGGSATRTPPARLGVAVVSDLVAAIVTGEL
                     APGDALPPESTLSQRFGVSRTVIRESVKRVEEKGLVTVAQGRGTTVNAPTAWNVLDPI
                     VLSALVDHDDTLGVLDELSIVRGSLESSMAAAAAARQNPQRSADLTRAFEETERTMND
                     IEAYADADARFHYLVMEQSGNRLAANITQILFQRARVSRRFTGTASPEAVQLTLEEHR
                     AILDAITAGDSEAASRAMHDHIARAWERRRPTDRRPTD"
     gene            complement(7429..7758)
                     /locus_tag="PLW15_04065"
     CDS             complement(7429..7758)
                     /locus_tag="PLW15_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOT33611.1"
                     /translation="MTRRFGMVCRLHPDKRAEYLELHSAVWPGVEAMITECGIRNFTI
                     FVRGDVLFGYYEYVGDDYEADQARMAADPVSQQWWSRTDPCQFGFDDDAPEGARWQEL
                     DEIWHQD"
     gene            complement(7755..8105)
                     /locus_tag="PLW15_04070"
     CDS             complement(7755..8105)
                     /locus_tag="PLW15_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33612.1"
                     /translation="MPCSVANRAAESELLEHTATTSAFSHSVSFFSFVGNWNNYFLPY
                     VMVPGRKAPVQVGLAELLSNVPQFNPTNAASTTIDLPTLALATVVSVAPVLLIFLFSQ
                     RFLVSGMTAGGTKE"
     gene            8053..8982
                     /locus_tag="PLW15_04075"
     CDS             8053..8982
                     /locus_tag="PLW15_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT33613.1"
                     /translation="MCSRSSDSAARFATEHGIPRHYGDFAALCADPEVDAIYLGTPHG
                     THFAYASEAIKAGKNVLSEKPLTMTADEARELQRLSLEHGVLVMEAMWMKFSPSIRKA
                     QELVAAGAIGTPMIVQAGLGYPVPTNGPKRFWVPELGGGALYDMGVYTIALAQLFLGI
                     PESISCVGHTRADGVDYFEGITLTFANGAIAQLTTSITYSIPPRGYLGGTTGSIDFAE
                     PLFSPPAFRHMAADPPRPPVIEEYTFEKEGAGYVPMFRAAGEAILAGEVEHAIHPMSA
                     TIAVLETMETVRAQLLAQRDHNLPWTGPVGPAE"
     gene            complement(8989..9684)
                     /locus_tag="PLW15_04080"
     CDS             complement(8989..9684)
                     /locus_tag="PLW15_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT33614.1"
                     /translation="MNPTEGQLRRAIVTGGCSGLGAACVTRLRTDRIEVLTLDVSPEA
                     DIRVDITDTVAVQQAVSAAGPIDILVNSAGIVGPNKPLWEVDVDDWKRTFDVNVVGLF
                     AVTKAVIPGMIERGWGRIVSMASMAGKDGNPNLSAYSASKAAVIGLTKSLGKELAKTG
                     VIANAIAPAVIETPMNATTSPEVLAHITSLIPMGRVGRAEEVAALVSWLCSDEVSFST
                     GAVYDISGGRATY"
     gene            complement(9671..11386)
                     /locus_tag="PLW15_04085"
     CDS             complement(9671..11386)
                     /locus_tag="PLW15_04085"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HOT33615.1"
                     /translation="MTLRSAEWYAGSDRNAYIHRAWMRRGNPEDSLDGSKPQIAIANT
                     ASDLTPCNKHLDEVAESVKAGIWEAGGVPYNLPVVSLGETQVKPTAMLWRNMAAMAME
                     EMFRANPIDGLVLLGGCDKTIPALLMAAASVDLPAVVVPGGPMLTGHFRGEALGCGTD
                     VWRLSEEARAGTITNGQFLASEQSMIRSKGHCNTMGTASTMAVVAEALGMTIPGFAGT
                     PAPDARLLSLSHETGRLIVDMVAADRKPSDIMTRDSFLNAIIAVAAVGGSTNSVVHLL
                     AIAGRLGIDLTLDDFDAAGAGVPVMANLQPSGTYLMDDLYRAGGVLAVLAQVGELLHP
                     EPITVTGSPLVDYLADRPVYDDTVIMPRDAPLMANAGIAVLRGNLAPRGALVKPAAAT
                     AELLQHTGPAVVFDSIEDMRARIDEPDLEVTADSVLVLRGCGPKGYPGMPEVGNMPLP
                     RKLLEAGVRDMVRISDARMSGTAYGTVILHVSPEAAAGGPLGVVRTGDLITVDLENRT
                     LSMAVPDDELAARQESAATRAAYAAPTRGWAKLYVDHVMQADTGADLDFLVGGSGDRV
                     SRESH"
     gene            11473..>11697
                     /locus_tag="PLW15_04090"
     CDS             11473..>11697
                     /locus_tag="PLW15_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33616.1"
                     /translation="MIEYPAQAATPPTYELAEGVIWDDRAGLVRWVDLWEGRVLAGTL
                     TDDTLTVTETIVLGQAAAAVGLAHDGGLVVA"
BASE COUNT         1918 a         4020 c         3925 g         1834 t
ORIGIN      
        1 ggagcgctcc agttgctcag aagaatgtct caacgagatc gatgacctca ggatcggatg
       61 catccgcgct atcgatcacg ggcaccagcc gccacttgtc catcgctgta cacggatgcg
      121 agaggtcgag tcgaaggaca tcccccacgc tcacgtcggc cgtcggggcg agcggaagaa
      181 acccgtgctg atcattcatc ttcgatagcg ggccgtgtac ggatcgcggc atgtcgaggt
      241 cgtaggggaa gtcgcgcttt cccccgtcca gcagtgcgag ctctggctcg ggccgggaca
      301 gcacacggga ccagccgtgt atcgctgatc gcagcccggg cagaggagtg atgccccggt
      361 agaagccggc atcgtgcaat tgatatgccc cggatcgcac gatcacggtg gccgcaccca
      421 cgagaggcgt cagcgcctcg gcgacgaggt cgaagaacgc gctaccaccg gcggtcacga
      481 tgggttgctg tcgccagtga atggctgcgc tcagttccac gaggtcggag aggtactgct
      541 gcacagccgc aaggtccttc gctgaccgtg acccgcccat cgcaccctcg tagccgccga
      601 cgccgaccaa cctgagttcg ggcgcggcct ctgcggcggc ggcaacggcg agcgcttcgc
      661 gaatgctgcg cgcaccggtg cgcactccgc ccagttccac gacgacgtcg acgggacgcg
      721 cggcgcgacg gaggccagct cgcattgcat caacggcctc gatgctgtcc acccagcaga
      781 ggaactcgaa gtcaggatcg gcgagttcgg cgcccaacca acccagtgct acgggatcca
      841 cgaactcgtt cgccagtatc actcgcttga tacccactga acgcgccacc tgcacctgcc
      901 acggcgtggc gagagtgatc ccccacgcgc cggcgtcgag cacctgctgc cacagcttgg
      961 gagccatagt cgtcttgccg tggggggcga gttcgaatcc gcgctcgctc atccaccctg
     1021 ccatcagtgc gacgttcgac gtggcagcgg cggagtcgat cgacagcacg ggcgtggaga
     1081 agtgcgagag gcgaggggag gtgctcagaa actcctcctg gctgagcccc cagctcgccg
     1141 ccggaaatga tttggcccag ggtccaagat tcgcgttcat tccgctcacg atagtctgtg
     1201 gcataatttc gaatagtagt actgcgttca aacagatgga atattgcgtg aacccgcctc
     1261 gcacaggaca ggaccatggc tgaattcgac tccctcgtcg ccatcgtgac gggcggtgca
     1321 tccggaatcg gggctgccgt cgcgcgccga ctcgccgagg ggggcgccac cgtggccgtc
     1381 ttcgatctga agcctgccga agccgacggg cacttcgccg tcgccgtcga cgtgtcggac
     1441 gatgcgtcgg tggtcagcgc ggtggcggcg gtcgtcgaga cattcgggcg aatcgacatc
     1501 gtcgtcaaca acgcgggcgt cggggctcag ggcgacgtca ctgcgaacga cgacagcgag
     1561 tggcaccggg tgtgggacat caatgccgtc ggcatggccc gcgtcactcg cgcagcactg
     1621 ccccaccttc gtgtctcgcc gagcgcggcg gtggtgaacg tggcctcgat cgtcgcgacc
     1681 gctggagtgc cgcagcgagt gctctactcg gcgacaaagg gggccgtgct gtcgatgaca
     1741 cgggccatgg ccgctgacca tgtgcgggaa ggcatccgct tcaacgcagt caaccccgga
     1801 accgctgaca ccccctggat caatgccctg ctggccaccg ccgagcatcc cgcggctgaa
     1861 cgtgcggccc tcgaagcgcg tcagccccac gggcgacttg ttgctcccga ggagatcgcc
     1921 gacgctgttg cctacctggc gagcccacgc tccggttcga cgaccggaac atccattgcc
     1981 gtcgacgggg gaatgcagga actgcgcctg cggccggcat gacgtcatcc tctacgaccc
     2041 actagcacca caagccatca gaaggagcac gtcatgagac ttgtccgcat cggcgccgcc
     2101 ggttcagaga gccccggagt gctcgtcgac gacgacacgg tcgtcgacct gtccgatgtc
     2161 gtgggggact acgacgagaa cttcttcgcc tccggagccc tggcgaacct cggtgacgtc
     2221 gtcgccgacc gcgtgcgcgc cggacgcacc gagcccgtcg cgggtcgacg attcggcgcc
     2281 ccgatcgccc ggccgcacca gattctctgc atcggactca actacagcga ccacgccgcc
     2341 gagaccggcc aggctgtacc gaccgaaccc atcctgttca ccaagtcgcc gaacaccctg
     2401 atcgggcccg acgacgacgt gctcatcccg cgcgggtcca ccaagaccga ctgggaagtc
     2461 gagctgggca tcgtcatcgg ccggcacagc tcgtaccttg ccgatgaggc ggaggcctct
     2521 gcatccatcg ccggctacgt tctcgtcaac gacgtgagcg aacgcgcgtg gcagatggag
     2581 cgcaacggac agtggtcgaa gggcaagtcg gcgcccacat tcaaccccgc cggaccctac
     2641 ctcgtcactc ctgaggaagt gggtgatgtt ctcaacctca ggatgacact cgacgtcgat
     2701 ggcaggcggc ggcaggacgg gtcgaccgcg acgatgatct tcagtccggc gttcatcgtg
     2761 cactacctca gccagttcat ggctctcgag cccggcgacc tcatcaacac cggcactccc
     2821 cccggtgtgg gcatgggtca gaagccgccc acctacctgc gcggcggcga ggtcatgact
     2881 ctcagcatca cgcgactggg cacgcagacc cagcgcgtca tcgctgcctc ggcgagctga
     2941 tgtcgggcgc cctcagggag ggctagcgac cgtcgcgctt cgcctgacgc ttcgcgccct
     3001 gcgcgcgact cgaggcgtcg cgcttcacgc cgccggtcgt cttgctgtgc gagcggacgc
     3061 gtccggcacc caccgtgcca tcctgggtgc ccgccgattc cgggccggtc gacttgccgg
     3121 cccgggcccg cgcaagccgc tcatccttga gggcctctgc ggcgtcgtgc gccactcgag
     3181 ccagttcgcc actgacgatg gacagcgcct cctcgaacac gtacgcgcgc gcccgtgact
     3241 tgtcggactt ggatgcggca gtaccgcgcg cgccgacctc ggtgacaccg gatgctgcgc
     3301 cccgtcggta gttcttcgag tgcttgttgc gcgcccgccg cacgcgcttg tggagttcca
     3361 tgagaccgtc ttcgtcgagc agggcaaggc gctcgggctc aagctcgcgg atgaggtcgc
     3421 gctcggtgct cttcagcacc cacaagtact gttccatgag gcaatcctgc ccacagacct
     3481 cggatgcggt ctagcccaaa cggtagacgc gtgccgcggt cccaccgagg atcgcggcgc
     3541 gaacctcggc gggttgcgag tcgaggatgc cggtgaccgc atcccatgtg cgcgcgtagc
     3601 cgccggcgag ctcggagatc ggccagtcgc ccccgtacag gaggcggtcc gcgccgaaga
     3661 ggtcgagcgc gtcctcgacg aaggggcgca cggcatccac ggtccaggag tcgagcgggc
     3721 cgaccgaggc gtacagacct gagaccttcg cgaccgtgcg agggttggcg gcgcactcgg
     3781 cgagcaaggc gcgccactcg acgtgcgctg cctcgtcacg gccgatgggc ggcttgccca
     3841 ggtgatcgat cacgatggtg agcgacggat gccgttcacc gagcgtcgcg atgtcggcga
     3901 gcgccgcgta atccgccgtg gggaagtcga gcggcacacc ggcggcggcc agcagctcga
     3961 ggctgtcacc cacggcgggc tgcagcaccc actcgcgttc gcgctcgtgc accaggttgc
     4021 gcacgccgac cacgatcgcc tcgaagcgct gaacgtcgag tgccacctgc tcaggccggt
     4081 cgagcggagt ccaggccacc accccgagga ccgcgggctg ccgggcggca accgccagca
     4141 tcacgtcggt gtcctcggcg ttgtctgacg cctgcacgag caccgctccg ccgatgcccc
     4201 gctcggcgag cgccggcgcg atctcgtcga acgagatggt gcggttcacg gatcccaacc
     4261 cgtcgtcgag ccaggggtag ttcgctcggg acgggtccca gacgtgcagg tgcgagtcaa
     4321 tgatcatggc gggctccccg ttcatgccag cgacgggatg aatccggcgg cctcgaggtc
     4381 gctccaaagg gcatccggaa tcggtgccga gtcccggtcg agtccacctt gcatctgctc
     4441 tccgttgcgc gcgcccagaa cggcagacac gaccgccgga tgacgcagcg gaaactgcac
     4501 ggcggcatcc ggaagtgtca cgccgtgagc gccgcagagc tcggcgatag cgcgggcgcg
     4561 cgcgagcagc tcggcggggg cggcctggta ctcgtagtgc gcgtcatccg gaacgacggc
     4621 ccgcccgagc agccccgagt tgtagacgcc cgcggcgacg acgccgacgc cgcgctcgag
     4681 ggcgatcgga agcaggtcgt cgagcgcgcc ctgatccaga agggtgaagc gcccggccag
     4741 catcaccagg tcgacgtcgc actcgcgcac gaaccgggcg agcatcgccg actggttcat
     4801 gccggcgccg atcgcccgca cgacgccctg gtcgcgcagt tcgatgaggg tcgcgatgcc
     4861 ctcggtggat gcctgctccc agtggtgatc gggatcgtgc aggtacagca catccacccg
     4921 atccatcccg aggcgggtca ggctctgctc gagcgagcgc aggatgccgt cgcggctgaa
     4981 gtcccattcg cgacggtact cggcgggcac gtggaatccg tcatcgtcga gctgatgcgc
     5041 gttctccggt gttggcacga gggcgcgacc gaccttggtg gagacgacga actcgtcgcg
     5101 cggtcggccg gcgagcgcgg caccgagtcg gcgttcggcc agtcccagcc cgtagtgcgg
     5161 tgcggtgtcg aagtagcgaa cgccgcccgc ccacgcgaca tcgacggcgt cgaccgtctc
     5221 ggcgtcggtg gtctcgcggt agaggttgcc gaaggtggag gtgccgaggc cgatctcact
     5281 cagcgcgggc gctctgccca gtgtggtgct gcgaatatca cgacctcctc gtcacgtcgg
     5341 tttcatcgta caatctgatt gatgctacgt cgaacgtgtg atgtatgctg tttgaagcga
     5401 ctgaatgccg caactgtcta aacgtcaagg gcgatgatga agatcaccgg gtaccgctgc
     5461 cttcgcagct tccacgattg gggacgtccc gtcggtgatg tcaacggatt catcgagtcc
     5521 ggtgtcaccg aggtgccgat cgtcattctc gaaaccgacg agggcgtcac tggagtcgcg
     5581 accggctcgc accatgacct cgagcgactc ttcccggcca tcgagggcca ggaccctcgc
     5641 gccgtctccg ccctgtacga ccggatgctc gcccgcgtct tcaagaccgg tcacggtggc
     5701 gccacctaca gtggcatcgg aactctcgac accgccctct gggacatcaa ggccaagctc
     5761 gccggcgaac cgctgtggcg cctgctcggc ggcggcgacc gcttcgtcgc gggctacgcc
     5821 tcgggactgg acatcgcact cgacgacgac cagctcgcca gcttctaccg cacgatggcc
     5881 gaacggggct tcaccgccgg caaactgaag ggcggccggg tggtcgccga cgacatccgt
     5941 cggctgcaca tcatcgccga cgagttgcgc ggcaacgccg accaccccgc cctcatgctc
     6001 gatgccaacg aatcgtggaa tctcaagcag gccgtgcgtc acgtgaccgc cgtcgaggcg
     6061 gtgcacgacc tcacctggat cgaggagccg ctgcggcgct gggatgccgc ggggcacgcc
     6121 cgcctctccc gctcgatcaa agctgcggtc gcgaccggcg agaagctgac cggactcgac
     6181 cagtaccttc cgctcttcga ggccggagcg gtcgacgtcg ctcaggtggg aagcccggcc
     6241 ggtgttaccc acttcctgcg ggtcgcctac gccgcccaca gccgtgacct tcccatcagt
     6301 ccggtgggcc tcacagccaa cgcatcggtc gcccacgctg ccgcagcggt gcccaaccac
     6361 ctctccgccg aggtgcagga cctgggcggc cctttcggta tcgacatcga catggagtac
     6421 gccgacggcg gcatcgttct tggggatcgg cccggagccg ggctcacgat cgacgaagac
     6481 gcgatcgctg cccgcggaac cgccggtggc gcgtggctcg tgcagggtgg accccacgtt
     6541 cgccccaagc gcgctggcct gcgcatggtc gcagaggacc cgtcgtccct gcccggctga
     6601 gcggggaccg ggccttacac tgggacgcat gaccgaccac gctgctgacg ttcccgtgcg
     6661 gaactcggtt ctcggaggtt cggcgactcg cactccgccc gcccggctgg gcgtcgcggt
     6721 tgtgtccgac ctggtggccg cgatcgtgac cggcgagctc gccccgggcg atgcgcttcc
     6781 gcccgagagc accctcagcc agcgcttcgg cgtgagtcgc accgtcatcc gcgagtcggt
     6841 caagcgggtc gaggagaagg gtctcgtcac ggtcgcccag ggccgcggaa cgacagtcaa
     6901 cgcgcccacc gcctggaacg tgctcgaccc gatcgtgctg tcggccctcg tcgatcacga
     6961 tgacaccctg ggcgtgctcg acgagctctc gatcgtgcgc ggaagcctcg agtcatcgat
     7021 ggcggctgcc gccgccgctc gtcagaaccc gcagcgcagc gccgacctca ctcgcgcgtt
     7081 cgaggagacc gagcgcacga tgaacgacat cgaggcctac gccgatgcgg acgcccgctt
     7141 ccactacctg gtgatggagc agtcgggcaa ccggttggct gcgaacatca cgcagatcct
     7201 gttccagcgc gcccgagtga gccgacggtt caccggcacc gcgagccccg aggcggtgca
     7261 gctcaccctc gaagagcacc gagcgattct ggatgcgatc accgccggtg actcagaggc
     7321 cgcgtcgcgt gccatgcacg accacatcgc gcgagcctgg gagcggcgcc gacccaccga
     7381 ccgccgcccg accgactaga ccaccggggt gcagtccggc gcccgggctc agtcctggtg
     7441 ccagatctcg tccagctcct gccagcgcgc gccctcgggg gcgtcatcgt cgaagccgaa
     7501 ctggcaggga tccgtgcgag accaccactg ctgcgacacc gggtcggccg ccatgcgggc
     7561 ctggtcggcc tcgtagtcgt cgccgacgta ctcgtagtag ccgaacagca cgtcgccgcg
     7621 cacgaagatc gtgaagttgc ggatgccgca ctccgtgatc atcgcctcga cccccggcca
     7681 caccgccgaa tgcagctcga ggtactcggc ccgcttgtcg ggatggaggc ggcacaccat
     7741 tccgaaccgc cgggtcactc cttggtaccg ccggcggtca tccccgagac gaggaagcgc
     7801 tgggagaaca ggaagatcag cagcaccggg gcgacgctca ccaccgtggc cagggctagc
     7861 gtcggcaggt cgatcgtggt ggatgcggcg ttcgtcgggt tgaactgggg cacgttcgac
     7921 agcagctcgg ccagacccac ctgcacgggc gccttacggc ccggcaccat cacgtagggc
     7981 aggaagtagt tgttccagtt gcccacgaag ctgaagaagc tcacgctgtg cgagaacgca
     8041 gaggtcgtcg ctgtgtgctc gaggagttcc gactcagccg cccggttcgc aacggaacac
     8101 ggcatcccgc gccactacgg ggacttcgcc gccctttgcg cggatccgga ggtggacgcg
     8161 atctacctgg gcacgccgca cggcacgcac ttcgcctacg ccagtgaggc gatcaaggcg
     8221 ggcaagaacg tgctcagcga gaagccgctc acgatgacgg ccgacgaggc gcgcgaactc
     8281 cagcgcctgt ccctggagca cggagtgctg gtcatggaag ccatgtggat gaagttcagc
     8341 ccgagcatcc gcaaggccca ggagctcgtc gcggccggcg ccatcggaac tccgatgatc
     8401 gtgcaggcgg gactcggcta cccggtgccg acgaacggac cgaagcgttt ctgggtgccc
     8461 gaactcggcg gcggtgcgct gtacgacatg ggcgtctaca cgatcgcgct cgcacaactc
     8521 ttcctcggca tcccggagtc gatcagctgc gtcggccaca cccgtgccga tggcgtcgac
     8581 tacttcgagg gcatcacgct gacgttcgcg aacggcgcga tcgcccagct gacgacatcc
     8641 atcacctact cgattccccc tcgcggctac ctcggcggca cgaccggcag catcgacttc
     8701 gccgagccgc tgttctcgcc gcccgcgttc cgacacatgg cggccgaccc gccccggccg
     8761 cccgtgatcg aggagtacac cttcgagaag gagggcgccg ggtacgtgcc catgttccgg
     8821 gccgccggtg aggcgatcct ggctggcgag gtggaacatg cgatccatcc catgtcggcg
     8881 acgatcgccg tgctggagac catggagaca gtgcgcgccc agttgctcgc gcaacgcgac
     8941 cacaacctcc cctggaccgg accggtcggt cccgccgagt aggccgcgtc agtacgtggc
     9001 gcgaccccca ctgatgtcgt agaccgcgcc ggtggagaag ctgacctcgt cggagcacag
     9061 ccagctcacg agcgcggcaa cctcctcggc cctgccgacc ctgcccatcg gaatgagcga
     9121 ggtgatgtgg gcaagcacct caggggaggt cgtcgcattc atgggcgtct cgatcacggc
     9181 gggtgcgatc gcgttggcga tgactccggt cttggccagc tccttgccga gcgacttcgt
     9241 gagcccgatc acggcggcct tgctcgcgga gtaggccgac aggttggggt tgccgtcctt
     9301 gcctgccatg ctcgccatgc tgacgatgcg accccacccc cgctcgatca tcccgggaat
     9361 cacggccttg gtcaccgcga agagccctac gacgttgacg tcgaacgtgc gcttccagtc
     9421 gtcgacgtcg acctcccaca gcggcttgtt ggggcccact atgccggcgc tgttgacgag
     9481 gatgtcgatg ggtccggccg ccgagacggc ctgctgcacc gcgaccgtgt cggtgatgtc
     9541 gacccggatg tccgcctcgg gcgagacgtc cagggtgagt acctcgatgc ggtcggtacg
     9601 cagtcgcgtc acgcacgcgg cgccgaggcc gctgcatccg cccgtcacga tcgcccgccg
     9661 gagctggccc tcagtgggat tcacggctca cccgatctcc gctgccgccc acgaggaagt
     9721 cgaggtcggc accggtgtcg gcctgcatca cgtgatcgac gtagagcttg gcccagccgc
     9781 gcgtgggagc ggcgtaggcg gcccgcgtcg ccgccgactc ctggcgggcg gcgagctcgt
     9841 cgtcgggcac agccatcgac agggtgcggt tctcgagatc gaccgtgatc aggtcgccgg
     9901 tgcgcaccac cccgagcggt ccgcccgcgg cggcctcggg ggagacgtgg aggatcaccg
     9961 ttccgtaggc ggtgccgctc atgcgggcgt cgctgatgcg caccatgtcg cgcaccccgg
    10021 cctcgagcag cttgcgcggc agcggcatgt tgccgacctc cggcatgccg gggtagccct
    10081 tcgggccgca gccccgcagc acgagtacgg agtcggccgt gacctccagg tcgggctcat
    10141 cgatgcgggc tcgcatgtct tcgatcgaat cgaagacgac ggcgggaccg gtgtgctgca
    10201 gcagctcggc ggtggcggcg gcgggcttca cgagagcgcc gcgcggggcg aggttgccgc
    10261 gcagcaccgc gatgccagcg ttggccatga gcggagcatc acggggcatg atcacggtgt
    10321 cgtcgtacac gggtcggtcg gcgaggtagt cgacgagggg cgatcccgtg acggtgatcg
    10381 gttcgggatg cagcagctcc cccacctgcg cgagcaccgc cagcaccccg ccggcccggt
    10441 acaggtcgtc catcaggtac gtgccgctcg gctgcagatt ggccatcacg ggaacacccg
    10501 cgcccgcggc atcgaagtcg tcgagcgtga ggtcgatgcc gaggcgaccg gcgatggcca
    10561 gcaggtgcac gaccgagttg gtcgagccgc cgacggccgc gaccgcgatg atcgcgttga
    10621 ggaacgagtc gcgggtcatg atgtcgctgg gtttgcgatc ggccgcgacc atgtcgacga
    10681 tcaggcgtcc ggtctcgtgt gagagcgaga gcaagcgcgc gtcgggggcg ggcgttcccg
    10741 cgaagccggg gatggtcatc ccgagtgcct cggcgacgac ggccatggtc gaggccgtgc
    10801 ccatggtgtt gcagtgcccc ttggagcgga tcatcgactg ctcggatgcc aggaactgcc
    10861 cattggtgat ggtgccggcc ctcgcctcct cgctgagacg ccacacgtcg gtgccgcagc
    10921 ccagtgcctc gccacggaag tggccggtga gcatcggccc gccgggcacg acgacggccg
    10981 gcaggtcgac ggatgccgcg gccatcagca gcgcgggaat cgtcttgtca cagccgccca
    11041 gcagcaccag cccatcgatc gggttggcgc ggaacatctc ctccatggcc atcgccgcca
    11101 tgttgcgcca cagcatcgcg gtgggcttga cctgggtctc gccgagcgag accaccggca
    11161 ggttgtaggg caccccgccg gcctcccaga tgccggcctt gactgactcg gcgacctcgt
    11221 cgaggtgctt gttgcagggg gtcagatcag acgcggtgtt cgcgatggcg atctggggtt
    11281 tcgagccgtc gagggagtcc tcgggattgc cgcggcgcat ccaggcccga tggatgtagg
    11341 cattgcgatc ggatccggcg taccactcgg cgcttctcag ggtcatcctt gaccttttcc
    11401 attagtttga atactgagct aacatgctga attatcgcat acgcgtcctg acggcatcga
    11461 ggcacgggag ccatgatcga gtaccccgca caagcagcga ctccgcccac ctacgagctc
    11521 gccgagggcg tcatctggga cgaccgggcg ggcctcgtgc gctgggtcga tctctgggag
    11581 gggcgagtgc tcgctggtac cctcacggat gacacgctca ccgtgaccga gacgatcgtg
    11641 ctcggtcagg cggccgccgc cgtcgggctc gcccacgacg ggggtctggt ggtcgcc
//
LOCUS       DAOSOU010000047        11655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5495_length_11655_cov_6.277155, whole genome shotgun sequence.
ACCESSION   DAOSOU010000047 DAOSOU010000000
VERSION     DAOSOU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_11655_cov_6.277155"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLW15_04095"
     CDS             complement(<1..286)
                     /locus_tag="PLW15_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT33617.1"
                     /translation="MIRFAAPEVSAADEAAVLRALRSGWLSTGDECALLEEELAAFCG
                     ARHAVALSSCTAALDIATASLRLRPGARVGVPTWTFAATAQPAAQLGAIPV"
     gene            complement(319..807)
                     /locus_tag="PLW15_04100"
     CDS             complement(319..807)
                     /locus_tag="PLW15_04100"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOT33618.1"
                     /translation="MPSFDISSEVDEQEMRNAVDQASRELANRFDFKDTNSSIELGPK
                     EITMRSVSQDRLNAVRQVLEEKLAKRKISMKALDYGTVEDAAGGTVRQVANLVAGINR
                     EKATELNKFIKALGLKGIQSQTQGEEVRVIAKKRDDLQAVIQACKEHDFGIPLQFGNF
                     RD"
     gene            922..1004
                     /locus_tag="PLW15_04105"
     tRNA            922..1004
                     /locus_tag="PLW15_04105"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:956..958,aa:Tyr,seq:gta)
     gene            1088..1579
                     /locus_tag="PLW15_04110"
     CDS             1088..1579
                     /locus_tag="PLW15_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOT33619.1"
                     /translation="MTLDPTSLPPSALRVSGILESLGHSGEVTMLADSARTAAEAAAA
                     LGCSVAEIAKSIVFRRRSDDAAVVVITSGDNRVDGTKVAALAGEVGKADADFVREATG
                     YAIGGVSPVGHPEATVLLVDRDLFRFETIWAAAGHPHAVFPLTPTQLVDWLGVEAEDV
                     RVD"
     gene            1635..2771
                     /locus_tag="PLW15_04115"
     CDS             1635..2771
                     /locus_tag="PLW15_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33620.1"
                     /translation="MNASELCDELERIYDGGDYPALTVGRPWSSHNPTLVGELARPSA
                     LRWYLVRELERLAEAGADLSVSPSRPRIALDDPAMFERSDESNWDLKRKKLFLFPPER
                     MELSLNRLSHYTGTEAEDFQRYVLFTNYHMHVEAFLELYPDCIKPSRANVQMPAFHHV
                     LDGHTGVTLVNIGVGPSNAKTITDHVAVLRPDLMMMIGHCGGLRNRQEIGDFVLATTY
                     LRADQVLDDVLPPHVPIVSNFQLNQLLYEELERRDIPFRMGTVFTTSNRNWEFNQRSP
                     LAEISASRAVATDMESATVAANGFRYRIPSSTLLVVSDKPLHGQPKLEGPAREFYESS
                     KRQHVEVALAAVAHVRSIFPNGLPSADLRSSDEPLMGGPADGDD"
     gene            2923..4209
                     /locus_tag="PLW15_04120"
     CDS             2923..4209
                     /locus_tag="PLW15_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT33621.1"
                     /translation="MQVLMAVIGLVMVGLLGLAGPAGASVRQDEATGEAIAGTFRNND
                     DGGKPVAGVEVVVRDDAGKEIGTATSDDEGKYELELPGPGAYTAELQTDSLPKGVNLT
                     DAKRTKLSFSVNPSQRKPLIFPFGKDTRVTVSTLDKIPGTLFDGARLGLIIAMTAIGL
                     SLIYGTTQFTNFAHGEMVTFGAIITWYLNQEAGLPVLWSALIAFVLAGAGGYALDTGL
                     WGPLRHKRTGPFSLMVVSFGLSIALIGTFQFFFGSRTKPYSEYVIQEPLFTIGNQVIP
                     PRAVVTMVLSVVVLGAVGLFLQRARFGKAMRAVADNGPLASASGINTDRVVKLVWIGG
                     SALAGLGGILYSLDQGVNVQQGQSILLLMFAGITLGGLGTSFGAAAGCFVLGVAIQVS
                     TLWIPTSLKNVGALVILIVILMIKPQGVFGRKERVG"
     gene            4209..5219
                     /locus_tag="PLW15_04125"
     CDS             4209..5219
                     /locus_tag="PLW15_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT33622.1"
                     /translation="MDWSQILTDTFSASIGVLAIVYVLAAQGLNIHYGYTGLFNFGQA
                     GFMAVGAYGLAIVTAPESDGGFGLSLWLGVVVAVLAAIVLALLMGIPTLRLRGDYLAI
                     VTIAVSEIIRLVLRSEWSDQNLSTGGNSGINGFARPFYDANPFVNSKRYELGPFQYSG
                     RELWVMLVGWGVVLFFAVLTFLLMRSPWGRVLRSIRENEDAVRSLGKNVQGYKMQALV
                     LGGIMGAFGGVILAIFQNLALPDDFVTRTTFLAYAALILGGAARVGGPIVGGIIWYTL
                     FSFMNNLVTQMKAADVFPSGVKPNAGQLPFIFLGLALMALVIFRPQGIFGDRKEVELD
                     AR"
     gene            5209..6150
                     /locus_tag="PLW15_04130"
     CDS             5209..6150
                     /locus_tag="PLW15_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33623.1"
                     /translation="MPADAPTASAGVTGPAGLVGLEPVVGVAKPDPIVVADGVVRRFG
                     GVTAVNVDHVEFQRGAITALIGPNGAGKTTFFNLLTCFDQPQEGDWSFNGESLKGIAP
                     HVVARRGLIRTFQLTKVLTKLTVIENMRLGATGQKGEKFLASLFRPGWKAQEDANTAR
                     ADELLVRFKMDHMREQFAGQLSGGQRKLLEMARALMTEPELIMLDEPMAGVNPALKQS
                     LLEHVKSLRAEGRSVIFVEHDMDMVFDISDWVVVMAEGRVIAEGTPEQVGQNQAVIDA
                     YLGSHHDAELDSIVHGEVPDHEVPVESDPNTDEGSPT"
     gene            6147..6893
                     /locus_tag="PLW15_04135"
     CDS             6147..6893
                     /locus_tag="PLW15_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33624.1"
                     /translation="MTVTDSPTPLLIADNLVAGYVPGVDILNGCSMELFHQEMVGIIG
                     PNGAGKSTFLKSLFGLIPIRSGDITLHGESIAGLKAHELVTKGVGFVPQTENVFQSMT
                     VRENMEMGAFLNPKAVATRFAEVCDLFPRLGERANQTAGSLSGGERQMVAMGRALMMS
                     PAVLLLDEPSAGLSPAFQDDVFLNCRKINQVGVSIVIVEQNAARCLQVCDRAFVLDHG
                     TNAYSGTGKELLNDPKVIELYLGTLAKSRK"
     gene            complement(7112..8491)
                     /locus_tag="PLW15_04140"
     CDS             complement(7112..8491)
                     /locus_tag="PLW15_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33625.1"
                     /translation="MQSKQLLARSVALLMSLSLVAVGCGDDKADDTSGDSDSTATTTT
                     AAEAASDGVLAGMKGTTPLVDLGDDFKNRMKEIDPALVDFNYGAESYDATIIIALAAA
                     EAKTDGAAYAEKINGITRGGEKCTDYKSCLEIINAGGDPDYDGASGPLEFSGNGEPTQ
                     ASYGILQFGEEKNSDDGTACDKTKECLDDALTKFVKATAPESADVPQVPVTATREGDG
                     ELTIGTLLPITGSLAFLGPPEFAGVKLAIKEINENGGYNGKDVKYIEGDSGDTENKVA
                     PATVATLLSQNADAIIGAASSSVSLSVVDTIVTAGVVMFSPANTSKKLSTYDDKGLYF
                     RDAPSDILQGQVLADTMIEDGKSNVYILALNDDYGTGLAEDLKAGLEGGGATVIDTKI
                     YDPKAADYSAEVSAAKDSGADAIALIGFDESSKILAKMAEQGIKLSIVYGVDGNMGNA
                     LAENFEAGK"
     gene            complement(8714..9205)
                     /locus_tag="PLW15_04145"
     CDS             complement(8714..9205)
                     /locus_tag="PLW15_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOT33626.1"
                     /translation="MIRVATTDDAEAIRSIYNLEVETSKVTFDIVPRSLTEQRRWILD
                     RSGAHAAIVAERAGIVVGFGSLSPYKERAAYSTSVEDSVYVHRDHHGTGVGRLLLTEL
                     VDRATAHGFHALFARIVAGHAASIGLHTSLGFEVVGNEREVGRKFGTWLDVTVMELIL
                     SDR"
     gene            complement(9232..10560)
                     /locus_tag="PLW15_04150"
     CDS             complement(9232..10560)
                     /locus_tag="PLW15_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33627.1"
                     /translation="MRLERLVIEADQNTFALDLHPRLTVIGGVGRLEREGLVNELVGA
                     LAGGRQGVHLELQADNGSRFAIFRPSGARHRIVEIDSRLDVTDQFADEDGMVNLLART
                     GLDARRAKASMRISSADLLTTSERDQLLQRLAHVNQNELWVAAETLRSAQRRLEDEAA
                     AVGSNAEDAEIIEQIEARHEEHERRRAAAERIRKATFVGSGLCAIGVVPAMLKVGLLA
                     AAPLIALALAGVLVSIVSWRRVLASARSEELALTEFGAQSYLGFHIQRVNGLLSSDQA
                     RRKLMQAAEEHREALRRWAVIAGDVELDWVLRNRDQIDAAARIRSEVFGGERNAEAGQ
                     AERAAGLAHTVVSRLAGLRTIGVGGESFVALFDEPFSDLEPAIVPPLLELLIRSSEHQ
                     QIVLLTEDPTIASWARLEAMTGNMSLVEPSPEPSTSSEVALWDPSVVHLR"
     gene            10745..10817
                     /locus_tag="PLW15_04155"
     tRNA            10745..10817
                     /locus_tag="PLW15_04155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10778..10780,aa:Thr,seq:ggt)
     gene            10946..11022
                     /locus_tag="PLW15_04160"
     tRNA            10946..11022
                     /locus_tag="PLW15_04160"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10980..10982,aa:Met,seq:cat)
     gene            11052..>11655
                     /locus_tag="PLW15_04165"
     CDS             11052..>11655
                     /locus_tag="PLW15_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT33628.1"
                     /translation="MAKEKFERNKPHVNVGTMGHIDHGKTTLTAAITKTLFDRVPGNS
                     FTAFENIDKAPEERERGITINVSHVEYETESRHYAHVDMPGHADYIKNMITGAAQVDG
                     AILVVSAADGPMPQTREHVLLARQVGVPAIVVALNKVDMVDDEELLELVELEVRELLS
                     SYEFPGDDIPVMPVSALKALEGDSAAQDQVMALMDAVDSYI"
BASE COUNT         1847 a         3725 c         3925 g         2158 t
ORIGIN      
        1 gcaccggaat cgcgccgagc tgtgccgcgg gttgggcagt ggcggcgaac gtccaagtcg
       61 gaaccccgac ccgtgcccca ggccggaggc ggaggctggc ggtggcgatg tcgagtgctg
      121 cggtgcacga cgacaacgcg actgcgtgcc tcgccccgca gaatgcagcc aattcctcct
      181 cgagcagcgc gcattcgtcg ccggtcgaga gccatccgct ccgcagggcg cgaagcaccg
      241 ccgcctcgtc ggccgcactc acctcaggcg cggcgaatcg gatcacgggg tctccaggtg
      301 acgtcaggac ggatcccgtc agtcccggaa gttgccgaac tggaggggga tcccgaagtc
      361 gtgctccttg cacgcctgga tcaccgcttg caggtcgtcg cgcttcttcg cgatcacgcg
      421 gacctcttcg ccttgggtct gtgactggat gcccttgagg ccgagcgcct tgatgaactt
      481 gttgagctcg gtggccttct cgcggttgat gcccgcgacg aggttcgcga cctgccggac
      541 ggtgccaccc gcggcgtcct cgacggtgcc gtagtcgagc gccttcatcg agatcttgcg
      601 cttggcgagc ttctcctcga gcacctggcg gacggcgttg aggcggtcct ggctcaccga
      661 acgcatggtg atctccttgg gaccgagctc gatggaggag ttggtgtcct tgaagtcgaa
      721 ccggttggcg agctcgcgcg aggcctggtc gacggcattg cgcatctcct gctcgtccac
      781 ctcggaactg atgtcgaagc tgggcacggg tactcctcgt ggtggtgatc gtcggtcggc
      841 cgtgacctcg gccgatctcg gcgcaccacg ctatcgactt ctccacccgc ccaacagggc
      901 gctaacctgt ccgacgttcc agggtcggtg cccgagtggc caaagggaac tggctgtaaa
      961 ccagtcggcg taagcctacg gaggttcaaa tccttctcgg cccacagtga gcacgcgaac
     1021 accccgcacc tcggtgcggg gtgttccggt tttcgtgtca cccgttgcgc ggcgagactg
     1081 cacggccatg acgctcgatc ccacctcgct cccgccgtcg gccctccggg tgtccggcat
     1141 cctcgaatcg ctcggtcaca gcggcgaggt cacgatgctc gccgattcgg cacgtactgc
     1201 agccgaggcg gctgcggcgc tgggttgctc ggtcgcggag atcgccaagt cgatcgtgtt
     1261 ccggcggcgg agcgacgacg ctgcggtggt cgtgatcacc agcggcgaca accgggtgga
     1321 cggcacgaag gtcgccgcgc tcgccggaga ggtcgggaag gccgacgccg acttcgtgcg
     1381 ggaggcgacg ggttatgcga tcggcggcgt gagcccggtg ggccaccccg aggcgaccgt
     1441 gctcctggtc gaccgcgatc tgttccggtt cgagacgatc tgggccgctg ccggccaccc
     1501 ccatgctgtc ttcccgctga cgccgaccca gttggtggac tggctcggcg tcgaggccga
     1561 ggacgtccgc gtggactgag actgcccaac cggaagggcg gcgcggcgcg agaggcccct
     1621 gatacgttcg gtcgatgaac gccagtgaac tgtgcgacga gctcgagcgc atctatgacg
     1681 ggggcgatta tcccgccctc accgtcgggc ggccgtggtc cagccacaac ccgacccttg
     1741 tgggtgaatt ggcccggccg tccgcgctgc ggtggtatct ggtccgggaa ctcgaacgac
     1801 tggctgaagc cggggccgac ctgtcggtgt cgccatcgcg cccgcggatc gccctcgacg
     1861 acccggcgat gttcgagcga tccgacgaat cgaactggga cctcaagcgc aagaagctgt
     1921 tcctgttccc acccgagcgc atggagctgt ccctcaatcg gctctcgcac tacacgggca
     1981 ccgaggcgga ggacttccag cgctacgtgc tcttcaccaa ctaccacatg catgtcgagg
     2041 cgttcctcga gctctacccg gattgcatca agccgagtcg agccaacgtg cagatgccgg
     2101 cgttccacca tgtgctcgac ggccacaccg gggtcacgct cgtgaacatc ggggtgggcc
     2161 cgtcgaacgc caagaccatc accgaccacg tcgccgtgct ccgccccgat ctcatgatga
     2221 tgatcgggca ctgcggggga ttgcggaacc gtcaggagat cggcgacttc gttctggcca
     2281 cgacctacct gcgtgccgac caggtgctcg acgacgtgct gccgccccat gtgccgatcg
     2341 tctcgaactt ccagttgaac cagctgttgt acgaggaact ggaacgtcgg gacatcccgt
     2401 tccgcatggg cacggtgttc accacgtcga atcgcaactg ggagttcaac cagcggtcgc
     2461 cactcgcgga gatctcggcc tcgcgggccg tcgcgaccga catggaatcg gcgacggtcg
     2521 cggccaacgg gttccggtat cggattccgt cgtccaccct gctggtggtg agcgacaagc
     2581 cgttgcacgg gcaaccgaag ctcgagggcc cggctcggga gttctacgag tcgtcgaagc
     2641 gtcagcacgt cgaagtagcg ctcgctgcgg tcgcccacgt gcggtcgatc ttcccgaacg
     2701 gtctaccgag tgccgatctc cgatcgagcg acgaacccct catgggaggc cccgccgatg
     2761 gcgacgactg aggtgacggg ggttgctggg gagttgcctc agcgtggaca acttcgtaac
     2821 gcatcggaaa cagccagggg ttatagtccg caaatcgtgc cgaccgatgc tctcgttcct
     2881 gccacgtcca ccatcgttcg gcctcggtct cagacacacc gcgtgcaggt gctgatggcc
     2941 gtcatcggtc tcgtgatggt ggggctgttg ggcctcgccg gcccggccgg ggcatcggtc
     3001 cgccaggacg aagcgaccgg cgaggcgatc gccggtacct tccgaaacaa cgacgacggc
     3061 ggcaagcccg tggccggcgt cgaggtggtg gtgcgcgatg acgccggcaa ggagatcggc
     3121 acggcgacgt ccgacgacga gggcaagtac gagctcgagt tgcccggccc cggcgcctac
     3181 accgccgagc tccagaccga ctcgctcccg aagggcgtga acctgaccga cgccaagcgg
     3241 accaagctct cgttctccgt caacccgtcg cagcgcaagc cactcatctt cccgttcggc
     3301 aaggacaccc gtgtcacggt gtcgacgctc gacaagattc ccggcacgct gttcgacggc
     3361 gcccgcctcg gtctcatcat cgccatgacg gccatcgggc tgtcgttgat ctacggcacg
     3421 acacagttca cgaacttcgc gcacggcgag atggtgacgt tcggggcgat catcacgtgg
     3481 tacctgaacc aggaggccgg gctgcccgtg ctgtggtcgg cgctgatcgc gttcgttctc
     3541 gccggagccg gcgggtacgc gctcgatacc ggcctgtggg gtccgctgcg ccacaagcga
     3601 accggcccgt tctccctgat ggtcgtgtcc ttcggcctgt cgatcgcact catcggcacg
     3661 ttccagttct tcttcgggag ccgtaccaag ccctacagcg agtacgtcat ccaagagccg
     3721 ctcttcacga tcggcaacca ggtcatccct ccccgggcag tggtcaccat ggtgctgtca
     3781 gtggtggtac tcggagcagt gggcctcttc ttgcagcggg cccggttcgg caaggcgatg
     3841 cgggcggtcg ccgacaacgg cccgttggcc tcggcctcgg gcatcaacac cgatcgggtc
     3901 gtcaaactgg tctggatcgg gggcagcgcc ctcgccggcc tcggcggcat cctctactcg
     3961 ctcgaccagg gcgtgaacgt ccagcagggc cagagcatcc tgctcctgat gttcgctggc
     4021 atcacgctcg gcgggctcgg caccagcttc ggcgccgccg cgggttgttt cgtcctcggt
     4081 gtcgcgatcc aggtctcgac actgtggatc ccgacgtcgc tcaagaacgt cggtgccctc
     4141 gtcatcctga tcgtcatcct catgatcaag ccccaagggg tgttcgggcg gaaggagagg
     4201 gtcggctgat ggactggtct cagatcttga cggacacgtt ctccgcgagc atcggtgtgc
     4261 ttgccatcgt gtacgtgctc gcggcgcagg gcctcaacat ccactacgga tacaccggcc
     4321 tgttcaactt cggccaggcg ggcttcatgg cggtcggcgc ctacggcctg gcgatcgtca
     4381 cagctcccga gtccgacgga ggcttcggtc tctcgctgtg gctgggagtc gtcgtggcgg
     4441 tgctcgcagc gatcgtgctc gcgttgctca tgggcatccc gacgctgcgg ctgcgggggg
     4501 actacctcgc gatcgtcacc attgcggtgt ccgagatcat ccggctcgtc ctgcgctcgg
     4561 aatggtccga tcagaatctc agcacgggtg gcaactccgg gatcaacggc ttcgcccgac
     4621 cgttctacga cgccaatccg ttcgtgaact ccaagcgcta cgagttgggg ccgttccagt
     4681 acagcggccg tgagctgtgg gtgatgctcg tcggttgggg cgttgtcctc ttcttcgctg
     4741 tgctgacctt tctcctgatg cgcagcccgt ggggccgggt gcttcgttcg attcgtgaga
     4801 acgaagacgc ggtgcggtcg ctcggcaaga acgtccaggg ctacaagatg caggccctgg
     4861 tgctcggcgg gatcatgggt gcgttcgggg gcgtcatcct ggcgatcttc cagaaccttg
     4921 cgctgcccga cgacttcgtg acccgcacca cgttcttggc ctacgccgcg ctcatcttgg
     4981 gtggcgcggc gcgagtgggc ggaccgatcg tgggcggcat catctggtac acgttgttct
     5041 ccttcatgaa caacctggtc acacagatga aggcggccga cgtgttcccg tcgggggtga
     5101 agccgaacgc cggacagttg cccttcatct tcctcggtct ggcgctcatg gcgctcgtca
     5161 tcttccgccc ccagggcatc ttcggtgacc gcaaggaggt cgagctcgat gcccgctgac
     5221 gcccccaccg cttccgccgg ggtgaccggg cccgccggac tcgtgggcct cgagcccgtg
     5281 gtgggtgtcg ccaagcccga ccccatcgtc gtcgccgacg gtgtggtgcg acgtttcggc
     5341 ggcgtcacgg cggtcaacgt cgaccatgtc gagttccagc gcggcgcgat cacggcgctc
     5401 atcggtccga acggcgccgg caagaccacg ttcttcaatc tgctgacctg cttcgaccag
     5461 ccgcaggagg gcgattggtc gttcaacggc gagtcgctca agggcatcgc gccccacgtc
     5521 gtcgcgcgtc gtgggctcat ccgcacgttc cagctcacga aggtgctcac caagctgacg
     5581 gtcatcgaga acatgcgcct cggcgcgacc ggtcagaagg gcgagaagtt cctggcctcc
     5641 ctgttccggc ccgggtggaa ggcccaggaa gacgcgaaca ccgcccgagc cgacgaactt
     5701 ctggtccggt tcaagatgga ccacatgcgc gagcagttcg ccggacagct gtcgggcggc
     5761 cagcgcaagt tgctcgagat ggctcgtgcg ttgatgaccg agcccgagct gatcatgctg
     5821 gacgagccga tggccggtgt gaacccggcg ctcaagcagt cgttgctcga gcacgtgaag
     5881 tcgctccgtg ccgaagggcg ttcggtcatc ttcgtcgaac acgacatgga catggtgttc
     5941 gacatcagcg actgggttgt cgtgatggcg gagggccggg tcatcgcgga gggcacgccc
     6001 gagcaggtgg gacagaacca ggcggtcatc gacgcctatc tcggcagcca ccacgatgcc
     6061 gagctcgact cgatcgttca cggcgaggtg cccgaccacg aggtcccggt cgagtccgac
     6121 cccaacaccg acgagggttc cccgacgtga ccgtgaccga ttctcccact ccactcctca
     6181 tcgccgacaa cctcgtcgcc ggctacgttc ccggcgtcga catcctcaac ggctgctcca
     6241 tggaactgtt ccaccaggag atggtgggca tcatcgggcc caatggcgcc ggcaagtcga
     6301 cgttcctcaa atcgctgttc ggtctgatcc cgatccggtc cggcgacatc acgttgcacg
     6361 gtgagtcgat cgccggtctg aaggcgcacg agctggtcac caagggtgtc ggtttcgtcc
     6421 cgcagaccga gaacgtgttc cagtcgatga cggtgcgcga gaacatggag atgggggcgt
     6481 tcctgaaccc caaggccgtg gcgacgcgct tcgccgaggt gtgtgacctg tttccccgcc
     6541 tcggtgagcg ggccaatcag acagctggct cgttgtcggg tggcgaacgt cagatggtcg
     6601 ccatgggccg tgcgctcatg atgagcccgg ctgtactcct gctcgacgaa ccatcggcgg
     6661 gattgtcgcc ggcgttccag gacgacgtct tcctcaactg ccggaagatc aaccaggtgg
     6721 gtgtgtccat cgtgatcgtc gagcagaacg cggcgcggtg cctgcaggtg tgcgaccggg
     6781 ccttcgtgct cgaccatggc accaacgcct actccggcac gggcaaggag ctcctcaacg
     6841 accccaaggt catcgagctc tacctgggca ccctggccaa gtcccgcaag tagccggggc
     6901 gccaaacgcg tcgcgagctt cgcgcgttcg cgattgcgct gcgacgcgtt tcgccggagc
     6961 gttcgggacg cgaaggaggc cggaccttgc ggcccggcct cctgtccgtt cactcggaac
     7021 gtaaccagtt cccagctact tgccggcttc gaagttctcg gccggtgtag ctctgtggcg
     7081 tcgacgcctg agcgtcgacg gcacaggttc gttacttgcc ggcttcgaag ttctcggcga
     7141 gggcgttacc catgttgccg tcgacgccgt acacgatgct gagcttgatg ccctgctccg
     7201 ccatcttggc gaggatcttg gacgactcgt cgaagccgat cagggcgatg gcgtcggcgc
     7261 cactgtcctt cgcggccgag acctcggcgc tgtagtcagc cgccttcggg tcgtagatct
     7321 tcgtgtcgat gaccgtggca ccgccaccct cgagacctgc cttgaggtct tcggcgaggc
     7381 cggttccgta gtcgtcgttg agcgccagga tgtagacgtt ggacttgccg tcctcgatca
     7441 tcgtgtcggc gagcacctgg ccctgaagga tgtcggacgg ggcgtcacgg aagtagaggc
     7501 ccttgtcgtc gtaggtcgac agcttcttcg aggtgttggc cggcgagaac atcacgacac
     7561 cagcggtgac gatcgtgtcg acgacggaca gcgagaccga ggaggacgcg gcgccgatga
     7621 tggcgtcggc gttctggctc agcagcgtgg cgacggtggc cggggccacc ttgttctcgg
     7681 tgtcgcccga gtcgccttcg atgtacttga cgtccttgcc gttgtagccg ccgttctcgt
     7741 tgatctcctt gatcgcaagc ttgacgccgg cgaactcggg cgggccgagg aaggcgagcg
     7801 agcccgtgat ggggagcagc gtgccgatgg tcagctcgcc gtcaccttcg cgggttgcgg
     7861 tgaccgggac ctgcgggacg tcggcgctct cgggggcggt cgccttgacg aacttcgtca
     7921 gtgcgtcgtc gaggcactcc ttggtcttgt cacaggcggt gccgtcatcg gagttcttct
     7981 cctcgccgaa ctgcaggatg ccgtaggagg cttgcgtcgg ttcgccgttg ccggagaact
     8041 cgaggggacc cgaggctccg tcgtagtcgg ggtcgccgcc ggcgttgatg atctcgaggc
     8101 acgacttgta gtcggtgcac ttctcgccac cgcgggtgat tccgttgatc ttctcggcgt
     8161 acgcggcgcc gtcggtcttc gcctcggcag ctgccagggc gatgatgatc gtggcgtcgt
     8221 aggactcggc gccgtagttg aagtcgacca gcgcggggtc gatttccttc atgcggttct
     8281 tgaagtcgtc gccgaggtcg acgagcggtg tggtgccctt catgcctgcg aggacaccgt
     8341 cgctcgctgc ctccgcggcg gtggtcgtcg tggccgtgga atcggagtca ccactcgtgt
     8401 catctgcctt gtcgtcgccg catccgacgg ctacgaggct gagggacatg aggagcgcaa
     8461 cgcttcgagc caacagttgc ttgctctgca ttcgtcaccc tttcggttgt gtgagttgct
     8521 gccagcccgg gaataccggg tggagtgcgg accaaaacct agtgaacttc gccccgcgag
     8581 gggtgcccga accgaattga cacacgatcg caacgaggcg tttcgcagca ttccgttgtt
     8641 cgccaaccga ggtaccgaaa cagaggcagc tcccgacgga gccgcgagcg acccggtgtc
     8701 ggggtgcccg aggtcatcga tcggacagga tcagctccat cacggtgacg tcgagccacg
     8761 tgccgaactt ccgcccgacc tcccgttcgt tgcccacgac ctcgaagccg agcgaggtgt
     8821 ggaggccgat tgaagccgcg tggccggcga cgatccgcgc gaacaatgcg tggaagccgt
     8881 gagcggtagc acggtcgacc agctcggtga gaaggagtcg cccgaccccg gtgccgtggt
     8941 ggtcgcggtg gacgtacacc gagtcctcga cgctcgtcga gtaggcggcg cgttccttgt
     9001 agggcgagag cgagccgaag ccgacgacga tgccggcgcg ctcggcgacg atggcggcgt
     9061 gcgcgccgga ccggtcgaga atccagcgcc gttgctcagt gaggctccgg ggcacgatgt
     9121 cgaaggtcac cttcgacgtc tcgacctcca ggttgtagat cgatcggatc gcctcggcgt
     9181 catcggtcgt cgccacacgg atcacggcga cgactgtaac ggtgctggga gctaccgcag
     9241 atggactacc gacggatccc agagcgcgac ctccgacgag gtcgacggct ccggtgaggg
     9301 ctcgacgagc gacatgttcc ctgtcattgc ctcgagccgg gcccatgacg caatcgtggg
     9361 gtcttcggtg aggagcacga tctgctggtg ctccgacgac cggatcagca gttcgagcaa
     9421 cggcgggacg atggcgggct cgaggtcgga gaacggctcg tcgaagagtg cgacgaagct
     9481 ctctccgccg actccgatcg tccgaagtcc ggcgagacgg ctgacgacgg tatgggcgag
     9541 gccggcggcg cgttcggcct gaccggcctc ggcgttgcgt tcgccgccga acacttcgct
     9601 gcggatacgg gccgcggcgt cgatctggtc gcggttgcgg aggacccagt cgagttcgac
     9661 gtccccggcg atgaccgccc aacggcgcag ggcctcgcgg tgttcctcgg ccgcctgcat
     9721 gagcttgcgg cgcgcctggt ccgatgacag gaggccgttc acccgttgga tgtggaagcc
     9781 gaggtagctc tgcgctccga actcggtgag cgccagctcc tccgaccgag cggaggcgag
     9841 gacccgtcgc catgacacga tactgacgag caccccggcg agcgccaggg cgatgagagg
     9901 agcggcggcc aggaggccga ccttcaacat cgccggcacc acgccgatgg cgcacaggcc
     9961 cgaaccgacg aacgtcgcct tgcggatgcg ttccgccgcg gcgcgacgtc gttcgtgctc
    10021 ctcgtggcgg gcctcgatct gttcgatgat ctcggcgtcc tcggcgttgg agccgacggc
    10081 ggctgcctcg tcctcgagcc ggcgttgggc tgagcgcagc gtctctgcag ctacccacag
    10141 ctcgttctgg ttcacgtggg ccaggcgttg aagcagctga tcgcgttcgc tggtcgtcag
    10201 caggtcggcc gagctgatgc gcatcgacgc cttggcgcgg cgggcgtcga gtccagttcg
    10261 ggcgagcagg ttcaccatcc cgtcctcgtc agcgaactga tcggtgacgt cgagccggga
    10321 gtcgatctcg acgatccggt ggcgagcgcc actcggtcgg aagattgcga accgggagcc
    10381 gttgtcggcc tggagctcga ggtggacacc ttgtcggccg cccgcgagcg ccccgacgag
    10441 ctcgttgacc agcccctcgc gttcgagccg ccccacgcca ccgatgacgg tcaggcgtgg
    10501 gtgcaggtcg agggcgaagg tgttctgatc tgcttcgatg acgagtcgct caagtcgcac
    10561 tcgcggttct tcggcggatc ccgacgcgaa acttgagcgg tttcactcat ctcgccgtgg
    10621 accgctggcc cctcggcgcg tgggccggtc gttcagtgcc aacccaaggc gcggattggt
    10681 tggagggccg cgtgggccgc ggctagagtt ccctccggtc atcgtcgcct tgcgccggtg
    10741 ccctgccccc ttagctcagt cggcagagcg cttccatggt aaggaagagg tcgtcagttc
    10801 aattctgaca gggggctcgg tactgtcccg accccttccg ggtcggaccc agtgcagctg
    10861 atcccgtcgg gatcgaacca ggtcccaccc ggatcccgag cggacccgga cccagcagtg
    10921 ccggctcgct cgtgagccgt tccccggtgg cgtagctcag ttggtgagag cactcggctc
    10981 ataatcgagg ggtcgtcagt tcaagtctga ccgccaccac caccccacaa cagagttgca
    11041 aggagttggc catggccaag gagaagttcg agcggaacaa gcctcacgtg aatgtgggga
    11101 cgatgggcca tatcgatcat ggcaagacga cgttgacggc ggcgatcacg aagacgttgt
    11161 tcgatcgggt gccggggaac tcgttcacgg cgttcgagaa catcgacaag gctcctgagg
    11221 agcgtgagcg tggcatcacg atcaacgtgt cgcatgtgga gtatgagacg gagagccgtc
    11281 actacgcgca tgtcgatatg ccgggtcacg cggattacat caagaacatg atcacgggtg
    11341 cggcccaggt tgatggtgcg atcttggtgg tgtcggctgc tgatggtccg atgccgcaga
    11401 cgcgtgagca tgtgctcttg gcccgtcagg tgggtgtgcc ggcgatcgtg gttgcgttga
    11461 acaaggtcga catggttgat gatgaggagc ttttggagct ggtcgagctc gaggttcgtg
    11521 agttgttgag ctcttacgag ttcccgggtg atgacattcc ggtgatgccg gtgtcggcgc
    11581 tcaaggccct cgagggtgat agtgcggcgc aggatcaggt gatggcgttg atggatgctg
    11641 tcgattcgta cattc
//
LOCUS       DAOSOU010000048        11488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5609_length_11488_cov_6.429983, whole genome shotgun sequence.
ACCESSION   DAOSOU010000048 DAOSOU010000000
VERSION     DAOSOU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5609_length_11488_cov_6.429983"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PLW15_04170"
     CDS             <1..1144
                     /locus_tag="PLW15_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HOT33629.1"
                     /translation="FQNQLAMPTNLHTHGLHVSPDGISDNVFLRIDPGASADYRYEIP
                     ANHPAGVYWYHPHHHGMAADQVFGGLYGAIIVEDPTPIDVTSERVLVISDMTFNSAGQ
                     IAAASQADRMNGREGATVLVNGQVAPAMTARPGDRERWRIINACSSRYLDLTLDGQQL
                     QLLGNDSGRFASPQGVTRLALVPGNRADVIVTMAAGSSVLRATPVDRGSAGMGGNTRS
                     TAVVDLATLDVTGVSTPSTAVEVASVTPRDLRGARVTGSRTLTLAMGGGGMGGGGMAF
                     TIDGATFDPATVNQRVALGSVEEWTIVNTSTMDHPFHLHVWPMQVLSVGGNSTSEPTW
                     QDVVNVPARSSSTVRIAFEDFGGTAVYHCHILDHEDNGMMGIISVA"
     gene            complement(1209..1847)
                     /locus_tag="PLW15_04175"
     CDS             complement(1209..1847)
                     /locus_tag="PLW15_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33630.1"
                     /translation="MRIVAPAALLVLPLAGCTAATAPAPATDAEVEEVLTSQFDARWD
                     ALGLASDIPKPDATTVDYTTRASWAYVQVACLQEQGVPAREVSGDFSIDPDTTHTRDE
                     SLIIQWTCQREFPVDPRLVGYLSIEQALHMYDYFTGRLAPCLELQGLAVPEPPARESY
                     VGALRAGIAWNPYYAADGTPLVATPADLAALDMRCPPLPDDPYGWYRPYDVG"
     gene            1947..2474
                     /locus_tag="PLW15_04180"
     CDS             1947..2474
                     /locus_tag="PLW15_04180"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOT33631.1"
                     /translation="MSDPFQNLQEKTGRTMPEWMVVVEASGREKHSDIMNWLKSEHGV
                     SHGFANGIALQFRSRDTVATDDQLVDAQFEGKKAALRPVYDRLIAVASELGDDVEVVP
                     KKTSVSLRRSKQFAVIEAASATRLQVGLQLRDHPTSDRLLAWGGMCSHKVNVSELADV
                     DDELVGWLREAYERN"
     gene            2519..2860
                     /locus_tag="PLW15_04185"
     CDS             2519..2860
                     /locus_tag="PLW15_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOT33632.1"
                     /translation="MTKYLISFPAAAMNLSPDEFVAAGEDSRAVIREAKAIGAYVFGG
                     GIDADVAPVMISGDGTVTAGTYPQTREFDGGFCVLELPSREDAEKWAAKIAAACRCAQ
                     ELREFMYDPES"
     gene            complement(2874..4055)
                     /locus_tag="PLW15_04190"
     CDS             complement(2874..4055)
                     /locus_tag="PLW15_04190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT33633.1"
                     /translation="MPRRRVALAFTAVLATATLLAGCSSPAPQPSATTTGDAAVTLTD
                     ETRATLQKLFDDGVAASGMPGAAAYVRIGDKEWTAVAGVSDLATQEPFDPADHVRIAS
                     NTKPFVATVVLQLEDEGALSLDDVLETYVPGIPNGDSITIRDLLQMSSGIWDLTSDDA
                     LMAQWADDLTMPWTVDESIALMRENPSRFEPGSKVVYTDSNYILLGRIAELVTGTSLA
                     ELVQSKILDALGLKETRFPTPDQADLPVPAAQGYRPPGEQLGSLDELISVGDINPEVA
                     WGAGNMSSTLGDLAIWADALADGTLLSPELQAERIAARQFDGQTIEFGYGLGIIRLGD
                     FLGHDGAIVGHSSAVFRLPADDATFVIVGNASSNFTTPTMDIFLSMVKELYPDQLRPV
                     E"
     gene            4209..4490
                     /locus_tag="PLW15_04195"
     CDS             4209..4490
                     /locus_tag="PLW15_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOT33634.1"
                     /translation="MQTLPISKVKDRLNELVDSASQTHERVTITKNGSPAAILIGMDE
                     WEALQETLFWLSQNDSRETIAQARAELANGEGLTEEQIRREFGVPKRSA"
     gene            4487..4753
                     /locus_tag="PLW15_04200"
     CDS             4487..4753
                     /locus_tag="PLW15_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT33635.1"
                     /translation="MSYEVVFAPTARREMSAVPERVTPAIIEFVYGDLASNPRRVGKA
                     LERELAGSFSARRGSYRILYDIDDEANRVLILRVAHRADVYRAS"
     gene            complement(4875..4948)
                     /locus_tag="PLW15_04205"
     tRNA            complement(4875..4948)
                     /locus_tag="PLW15_04205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4912..4914),aa:Thr,seq:tgt)
     gene            4998..5990
                     /locus_tag="PLW15_04210"
     CDS             4998..5990
                     /locus_tag="PLW15_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT33636.1"
                     /translation="MSSAAPSHPHPSRFVLIVAVGIAVISGALTALQTRVNSQLSLEL
                     GDGILASVISFASGLVILTIAVLVAPRGRDGLARLTTSVRERRTPWWYLIGGLFGAVF
                     VVGQGLTAAVLGVALFTIAAVATQTITGAVIDRVGLGDLPKRPVTALRVVASLLALAA
                     VTFSGLAELRVDVPIGLLILPLLAGVGIGFQQAITGQVRHVSQSALTATFINFLTGTT
                     VLILAAIVHTIVVGWVASFPSNPLLYTGGVLGVLFIGGAAVVVRTIGVLLLGLGTIAG
                     QLLGALVLDLVIPISGRGLPTTTVIGTLLTLVAVSLAVLSARRSHVATPPLTEP"
     gene            complement(5977..6531)
                     /locus_tag="PLW15_04215"
     CDS             complement(5977..6531)
                     /locus_tag="PLW15_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT33637.1"
                     /translation="MSSAAIDAVRRRQSHAVVTDAAPTHAELLPLVQAAASAADHGAL
                     HPWRLIELRGDARVRLGEAFVAESKLMGSDAQRLAEKPLRASLLIAVVARHEPSFKVA
                     EWEQDAAASGIAHLLSLLLAEAGWGVMWRTGGHTRSTAVRAVHQLADNEKLLGWLYVG
                     GVPEDGASKPPRTPIDPEDYLTVL"
     gene            complement(6528..6929)
                     /gene="msrB"
                     /locus_tag="PLW15_04220"
     CDS             complement(6528..6929)
                     /gene="msrB"
                     /locus_tag="PLW15_04220"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOT33638.1"
                     /translation="MDYQVTKSDDEWKAELTPEEYAVLRQAATERPWTGELLDENREG
                     VYKCKACGQELFRSTAKFDSHCGWPSFYIPSEQDAVELLRDSTLGMERVEVRCANCGS
                     HLGHVFDDAPQTPTGDRFCMNSVSLSFEAAE"
     gene            6986..7450
                     /locus_tag="PLW15_04225"
     CDS             6986..7450
                     /locus_tag="PLW15_04225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33639.1"
                     /translation="MPDSSVTVKPWAELTTAELYSFLKLRTDVFLVEQKVDEEELDNR
                     DLEPTTVHYFITDDAGAAAYLRTLHDDPPQYRDARRAIGRVVVRSDRRGEGLAQQLMT
                     MALADLAGEAVVLHAQQYIAPLYESHGFESFGEVYIEAGIPHISMYRPAVTA"
     gene            7447..8181
                     /locus_tag="PLW15_04230"
     CDS             7447..8181
                     /locus_tag="PLW15_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HOT33640.1"
                     /translation="MNVAALAIAAALAVGALAAAPAAAASSAAAAPDCTVTDATMTWG
                     FKESFRSYISGTIANGEWTVADGTTYETPDFGWANGTGQISDGVGQIAFTGSIEFTGH
                     GGILDTTVTNPRLVLDGSSRATLLLDVSGTTQQGADVDEKAVPFASVDISGAEFGDGT
                     VTITAAPTVLTEAGAAAFGTYEAGETLDPITAQLSADPACTIAPVGGPGLAGAIWLGI
                     GSVLAIVVITVELVLWRRRSAQEPGA"
     gene            complement(8163..9260)
                     /locus_tag="PLW15_04235"
     CDS             complement(8163..9260)
                     /locus_tag="PLW15_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33641.1"
                     /translation="MRTRTSVTAAVFALIAATLAGCAAAPTGTVDSAVTADPSAPLTE
                     LTFLTDPHSYVGQSTAILSTDAIPTPDTAPEQSLPATVSSHDLDGDVPVTVTSTDRIV
                     AMDLSGSIAATVYGLGLGDSMVGRDISTTFSQAADLPVITSSGHTVNSESILALRPTL
                     VITDGSIGPIDVVLQLREAGIPVVFVETTPSIDGVGELARQVSAALGAPAAGEVLAQS
                     LVSGIEAKVSEIASVVPSGKPLRMMFLYLRGTSGIYYLFGKESGADVIISALGGVDVA
                     AEIGLDGERPMTDEAVVAANPDLILVMTDGLASVDGVSGLLDSKPAIALTTAGQNRRF
                     VDMADGEILSFGPRTADVLDALARAIYAPGS"
     gene            9427..9744
                     /locus_tag="PLW15_04240"
     CDS             9427..9744
                     /locus_tag="PLW15_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HOT33642.1"
                     /translation="MPEPADRHASGLSVRDARILDFERQWWRHAGAKEEAIRSEFSLS
                     AARYYQLLNAVIDSPAAVRYDPMLIGRLQRARDARTSARAARAFRTSDRGGDSLHTEE
                     SND"
     gene            9748..10329
                     /locus_tag="PLW15_04245"
     CDS             9748..10329
                     /locus_tag="PLW15_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOT33643.1"
                     /translation="MASYPKDRFDQLPEDLQRIGAHRGPAKKGRGWIGFAWALLATGV
                     LIFGGLFAIGKVLGIDLGISIFAPAATPTPTPTPTPTMDPILDPTTIDQVARGIKIDV
                     LNGTPIVDLENTVGDKLAAAGWRMGAFTVASDKTQEATFVYYSDPLNEDVARGIVVAM
                     GIGEIRKVTPETFPGASITVVLGSDYPVPTAAP"
     gene            10527..10730
                     /locus_tag="PLW15_04250"
     CDS             10527..10730
                     /locus_tag="PLW15_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT33644.1"
                     /translation="MANGTVKWFNAEKGYGFITVDGGGQDVFVHYSAIDMNGYKVLEE
                     GQAVIFEVGTGAKGPQAESVKLA"
     gene            10898..>11488
                     /locus_tag="PLW15_04255"
     CDS             10898..>11488
                     /locus_tag="PLW15_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOT33645.1"
                     /translation="MAKIIAFNEEARRGLERGLNILADAVKVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELDDPYEKIGAELVKEVAKKTDDVAGDGTTTSVVLAQALVREG
                     LRNVAAGADPISLKKGIEKAVAAVIEALVAGAKEIETKEEIAATASISAGDSEIGALI
                     AEAIDKVGKEGVVTVEESNTFGTELELTEGMRFDKGF"
BASE COUNT         1898 a         3842 c         3837 g         1911 t
ORIGIN      
        1 cttccagaac caactggcga tgcccaccaa cctgcacacg cacggccttc acgtgtcgcc
       61 ggacggcatc agcgacaacg tgtttcttcg catcgacccg ggggcatcag ccgactaccg
      121 ctacgagatc ccggcgaatc atcccgctgg cgtctactgg taccacccgc accaccacgg
      181 tatggccgcg gatcaggtct ttggcgggct gtacggggcg atcatcgtcg aggatcccac
      241 gcccatcgac gtcacctctg aacgagtact cgtgatctct gacatgacct tcaactcggc
      301 gggacagatc gccgcggcat cccaggccga tcggatgaac ggtcgcgagg gtgcgaccgt
      361 gctcgtgaac ggccaggtcg cgcctgccat gactgcccgg cccggcgatc gggagcggtg
      421 gcgcatcatc aacgcctgtt cgtcgcgcta cctcgatctg acactggatg gtcaacagct
      481 ccagttgctc ggcaacgact ccggccgctt cgcctccccg cagggcgtca cccgccttgc
      541 gttggtgccc ggcaatcgag ccgacgtcat cgtgacgatg gccgccggta gctcagtctt
      601 gcgggcgacg ccggtcgatc gtggctcggc aggcatgggt ggcaacaccc ggtcgacggc
      661 cgtggtcgac ctagcgacgc tggatgtcac cggtgtgagc actccgtcga cggcggtcga
      721 agtcgcatcc gtcactccac gagacctgcg tggcgcccgg gtaacaggca gtcgcaccct
      781 cactctcgcg atgggcggcg gcggcatggg cggcggcggg atggcgttca ctatcgacgg
      841 ggcgaccttc gaccccgcca cagtgaatca gcgggtcgct cttggctcgg tggaggagtg
      901 gacgatcgtc aacaccagca cgatggatca tccgttccac ctccacgtat ggccgatgca
      961 ggtgctctcg gtaggcggga attcgacatc cgagcccacg tggcaggacg tcgtgaacgt
     1021 cccggcgcga agcagttcga cggtcaggat cgcgttcgag gacttcgggg gcaccgcggt
     1081 gtaccactgc cacatcctcg atcacgagga caacggcatg atgggcatca tctccgtcgc
     1141 ctgagctgag gctgatccgc gagatggagc gacctgcgcg ccgcgcgatg gctgaagagc
     1201 cgcgaccgct agcccacgtc gtacggccgg taccagccgt acgggtcatc cggcagcggt
     1261 gggcaccgca tgtcgagggc agccaggtcg gcgggggtcg ccacgagggg agtgccgtcg
     1321 gcggcgtagt acgggttcca cgcgatgccg gcccgcagcg cacccacgta ggactcgcgg
     1381 gcgggtggct ccggcacggc cagcccctgc agttcgaggc acggcgcgag tcgacccgtg
     1441 aagtagtcgt acatgtgcag tgcctgctct atcgagaggt acccgaccag gcgggggtcg
     1501 accgggaact cccgttggca ggtccactgg atgatgaggc tctcgtcgcg cgtgtgcgtg
     1561 gtgtccgggt cgatggagaa atccccgctg acctcccgcg ccggcacgcc ctgctcctgc
     1621 aggcacgcca cctgcacgta cgcccacgat gcgcgcgtcg tgtagtcgac tgtcgtcgca
     1681 tccggcttcg ggatgtcgct ggccagcccc aacgcgtccc acctcgcgtc gaactgactc
     1741 gtgagtacct cctcgacctc ggcgtcagtc gccggagccg gggcagtcgc ggcggtgcac
     1801 ccggcgagcg gcagcaccag gagggcggcg ggagccacga tgcgcatacg cccagtgttg
     1861 caccgggcgc tcgacgcgtg caccccgttc aggccgtcgg cgggcgcatc ttgtcgccgg
     1921 ccgccggtcg cggcagagta tccgccatga gtgatccgtt tcagaacctt caggagaaga
     1981 ccggacgcac gatgcccgag tggatggtcg tggtcgaggc atccggtcgc gagaagcaca
     2041 gcgacatcat gaactggctc aagtccgaac acggcgtcag tcatggcttc gcgaacggga
     2101 ttgcgttgca gttccgatct cgggacacgg ttgcgaccga cgaccaactg gtggacgccc
     2161 agttcgaggg caagaaggcg gcgctgcgcc ctgtatacga ccggctcatc gcggtggcca
     2221 gcgagctcgg cgatgacgtg gaggtcgtgc ccaagaagac ctcggtctcc ctgcgccgca
     2281 gcaagcagtt cgcggttatc gaggccgcga gcgcaacccg gctccaggtc gggcttcagc
     2341 tgcgcgacca tccgacctca gatcggcttc tcgcgtgggg cggcatgtgc tcgcacaagg
     2401 tcaacgtgtc tgagctggcc gacgtcgacg acgagctggt gggctggctc cgcgaggcct
     2461 acgagcgcaa ctgacgacac ccctcgacgg cagccgaggt catgtgtacg gtgttcacat
     2521 gaccaagtac cttatctcct tcccagccgc tgccatgaac ctctcgccgg acgaattcgt
     2581 cgccgctgga gaggactccc gcgccgtcat ccgcgaggcc aaggccatcg gtgcctatgt
     2641 cttcggtggg ggcatcgatg cggatgtcgc gcccgtcatg atcagcggag atggaacggt
     2701 cacggccggc acctacccgc agacccgcga gttcgatggc ggtttctgcg tgctcgagtt
     2761 gccctcgcgg gaggacgccg agaagtgggc cgcgaagatc gccgccgcct gccgctgcgc
     2821 gcaggagctg cgcgagttca tgtacgaccc ggagtcgtag gtctgacgaa gccctattcg
     2881 accgggcgca gttggtcggg gtagagctcc ttcaccatcg acaggaagat gtccatggtt
     2941 ggcgtagtga agttggatga cgcgttgccg acgatcacga aggtcgcgtc atccgcgggc
     3001 aggcggaaca cggccgaact gtgaccgacg atcgcgccgt cgtgaccgag gaagtcgccg
     3061 aggcggatga tcccaagccc gtagccgaac tcgatcgtct gcccatcgaa ctggcgggca
     3121 gcgatgcgtt ccgcctgcag ctcggggctg agcagcgtgc cgtcggcgag cgcatccgcc
     3181 cagatcgcga ggtcgcccag ggtcgacgac atgttgccgg cgccccacgc gacctcgggg
     3241 ttgatgtcgc ccacggagat gagttcgtca agtgagccga gctgctctcc cggcgggcga
     3301 tagccctggg cggcgggcac gggcaggtct gcctggtcgg gcgtggggaa gcgggtttcc
     3361 ttcagcccca gcgcgtcgag gatcttcgac tgaacgagct cggcgagcga ggtgcccgtg
     3421 acgagctccg cgatccggcc cagcaggatg tagttcgagt cggtgtaaac caccttcgag
     3481 ccgggttcga agcgcgacgg gttctctcgc atgagcgcga tcgactcgtc gaccgtccac
     3541 ggcatggtga ggtcgtcggc ccactgcgcc atgagcgcgt cgtcgctggt gagatcccag
     3601 atacccgaac tcatctgcag caggtcacgg atcgtgatgc tgtcgccgtt cgggatgccc
     3661 ggaacgtacg tctcgagcac gtcgtcaagt gacagcgcgc cctcgtcctc gagctgaagc
     3721 acgaccgtgg cgacgaaggg cttggtgttg ctcgcgatgc gaacgtggtc cgccgggtcg
     3781 aagggctcct gcgtcgccag gtcgctgacg cccgcgacag cagtccactc cttgtcgccg
     3841 atgcgcacgt acgcggcggc cccgggcatc ccggatgctg cgacgccgtc gtcgaagagc
     3901 ttctgcagtg tcgctcgtgt ctcgtcggta agcgtgaccg cggcgtcgcc ggtggtcgtg
     3961 gcggacggct gcggagcagg gctgctgcac ccggcgagca gggtggcagt tgcgagaacg
     4021 gcggtgaagg cgagggcgac gcggcggcgg ggcatcgaga gctccttgac gatcaggggt
     4081 cgctcgatgt tatcggcaga cggacttgag ggcacaggtc tggcgcctgg ctgtgctcaa
     4141 gcagcgaaga aagagggccc tcggtcttgc ttcttcgata ggcctgaatt caggcgtatc
     4201 atgacggcat gcaaacgctg ccgatatcga aggtcaaaga ccgtctcaac gaactcgtcg
     4261 actcggcctc gcagacgcac gagcgggtca ccatcacgaa gaatggcagc cctgccgcca
     4321 ttctgatcgg catggacgag tgggaggccc tgcaggagac tctcttctgg ctaagtcaga
     4381 atgacagtcg cgaaaccatt gcccaggctc gtgcggagct ggcgaacggt gagggtctga
     4441 ccgaagaaca gattcggcgt gagttcggcg ttcccaagcg ctcggcatga gctacgaggt
     4501 cgtcttcgcg ccgaccgccc gacgtgagat gtctgcggtg cctgagcggg tcaccccggc
     4561 catcatcgaa ttcgtctacg gggatctcgc ctcgaaccca cggcgtgttg gcaaggccct
     4621 cgaacgggag ctcgcgggat cgttcagcgc ccgtcgcggc agctatcgga ttctgtacga
     4681 catcgatgac gaggcgaatc gagtgctgat cctcagggtg gcccatcggg cggatgtcta
     4741 ccgcgcatcg tgacgttcgt cgcgctcgat agccgcgatc gtcgcgtttg ccgggcggct
     4801 ttcgaaccgc gagtgtggcg ggcaccggcc atgtgggctc cggccaaaat cgcgcattgc
     4861 gcgatttctg gcggagcccc ctgtcggatt cgaaccgacg acccccgctt tacaagagcg
     4921 gtgctctggc caactgagct aagggggcgt gaacgcccgc gcgaacactg cgcgcgaaca
     4981 tcgtccatag tagctaagtg agttctgccg cgccgtcgca tccgcacccc tcccgcttcg
     5041 tgctcatcgt cgcggtgggc atcgccgtga tcagcggcgc actcacggca cttcagacgc
     5101 gggtgaacag ccaactctcg ctcgagctgg gtgacggcat cctcgcgtcg gtcatctcct
     5161 tcgccagcgg gctggtgatc ctcacgatcg ccgttctggt cgcccctagg ggtcgggatg
     5221 ggctcgcccg cctcaccacg tcggtgcgcg agcggcgcac accctggtgg tacctcatcg
     5281 gcggactctt cggcgccgtc ttcgtcgtcg gtcagggcct cacggccgcc gtgctcggcg
     5341 tcgcactgtt cacgatcgcg gccgtcgcca cccagacgat cactggcgcg gtcatcgatc
     5401 gggtcgggct gggcgatctg ccgaagcgcc cggtaaccgc gctgcgtgtc gtggcatcgc
     5461 tgctggcact cgccgccgtc accttctccg ggctggccga actgcgcgtc gacgtgccga
     5521 tcggcctgct catcctgccg ctactcgccg gcgttggcat cgggttccag caggccatca
     5581 ccggccaggt gcggcacgtg tcgcaatcgg cgctgacggc gacgttcatc aacttcctca
     5641 ccggcaccac cgtgctgatc cttgcggcga tcgtgcacac gatcgtggtc gggtgggttg
     5701 cgtcgttccc cagcaacccg cttctctaca ccggcggggt gctcggggtg ctcttcatcg
     5761 ggggtgcggc ggtcgtcgtc cgcaccatcg gagtactgct tctgggcctg ggtacgatcg
     5821 ccggtcaact gctcggggcg ctcgtgctcg atctggtcat cccgatctcg gggcgcggtc
     5881 tgccgacaac gaccgtgatc ggcacgctgc tgactctcgt ggccgtatcg ctcgcggtgc
     5941 tgtccgcacg gcgctcacac gtggcaacgc cgccgctcac agaaccgtga ggtagtcctc
     6001 cgggtcgatc ggcgtgcggg gaggtttgct cgcgccatcc tcgggaacac cgcccacgta
     6061 caaccagccg agcagcttct cgttgtcggc cagctgatgc acggctcgca ccgcggtcga
     6121 gcgggtgtgc ccgccggtgc gccacatcac gccccagccg gcctcggcca gcagcaggct
     6181 gagcaggtgc gcgattccgg atgccgcggc atcctgctcc cactcggcca ccttgaacga
     6241 cggctcgtgc cgtgccacca ccgctatcag caggctcgcc cgcagcggct tctccgcgag
     6301 tctctgcgcg tcggatccca tcagcttgct ctccgcgacg aacgcctcac cgaggcgcac
     6361 gcgggcgtcg ccgcgcagct cgatgagccg ccacgggtgc agcgcaccgt ggtcggccgc
     6421 cgaggccgcg gcctgcacga gcggcagcag ctcggcgtgg gtgggcgcgg catccgtcac
     6481 cacagcgtgg gactgccgac gccgcaccgc gtcgatcgcg gctgagctca ctcggcggct
     6541 tcgaagctca gcgacaccga gttcatgcag aaccggtcgc cggtgggggt ctgcggtgcg
     6601 tcatcgaaga cgtgaccgag gtgcgagccg cagttggcgc agcgcacctc gacgcgctcc
     6661 atgccgaggg tcgagtcgcg cagcagctcg acggcgtcct gctcgctcgg aatgtagaag
     6721 ctcggccagc cgcagtgcga gtcgaacttc gcggtgctgc ggaacagctc ctgcccgcac
     6781 gccttgcact tgtagacgcc ctcgcggttc tcgtcgagca gctcgcccgt ccacgggcgt
     6841 tcggtggcgg cctgccgcag caccgcgtac tcctccgggg tcagctcggc cttccactcg
     6901 tcgtcgctct tggtgacctg gtagtccatg atgttgctcc tgcgctgggg aagagaagtc
     6961 gtcgttcaag cttaaacttt cagacgtgcc cgattcttcc gtgaccgtca agccgtgggc
     7021 cgagttgacc accgccgaac tctattcgtt cctcaagctt cgcaccgacg tgttcctcgt
     7081 cgagcagaag gtcgacgagg aagagctcga caaccgcgac ctcgaaccga ccacggtgca
     7141 ctacttcatc acggatgacg cgggcgcagc cgcctacctc cgaaccctgc acgacgatcc
     7201 gccccagtac cgcgacgcgc gccgggccat cggccgggtc gtggtgcggt ccgaccgccg
     7261 cggtgagggc ctcgcccagc agctcatgac catggccctc gcggatctcg cgggcgaggc
     7321 ggtcgtgctg cacgcgcagc agtacatcgc acccctctac gaaagccacg gattcgagag
     7381 cttcggcgag gtgtacatcg aggcgggcat cccgcacatc tccatgtacc gcccggcggt
     7441 gacggcgtga acgtcgccgc tctggccatc gcggcggcac tcgcggtcgg cgccctcgcg
     7501 gccgcacctg ccgcagccgc atcatcagca gcggccgcgc ccgactgcac tgtcaccgac
     7561 gcgaccatga cgtggggctt caaggagtcg ttccgcagct acatctccgg cacgatcgcc
     7621 aacggggagt ggacggtcgc cgacggcacc acctacgaga cccccgactt cggctgggcc
     7681 aacggcaccg gccagatctc cgacggtgtc gggcagatcg ccttcacggg gtcgatcgag
     7741 ttcaccggcc acggcggaat ccttgacacc acggtcacca acccgcggct cgtgctcgac
     7801 ggctcgtcac gggcgaccct gctgctggat gtctccggca cgacccagca gggtgccgac
     7861 gtcgacgaga aggccgttcc cttcgcgtcg gtggacatct cgggcgccga gttcggcgac
     7921 ggcaccgtca cgatcaccgc ggccccgacc gtgctcaccg aggcgggtgc ggccgcgttc
     7981 ggcacctacg aggcaggcga gacgctcgat ccgatcaccg cgcaactgag cgccgacccc
     8041 gcgtgcacca tcgcaccggt gggtgggccc ggtcttgcgg gtgcgatctg gttgggcatc
     8101 gggtcggtgc tggcgatcgt cgtgatcacc gtcgaactcg tgctctggcg tcgccgcagc
     8161 gctcaggagc cgggcgcgta gatcgcccgg gcgagcgcgt cgagcacgtc ggcggtgcgc
     8221 ggtccgaagc tcaggatctc gccgtcggcc atgtccacga accggcggtt ctggcccgcg
     8281 gtcgtgagcg cgatggcggg cttggagtcg agaagcccgc tgacgccgtc gacgctggcc
     8341 aggccatccg tcatcacgag gatcaggtcg gggttcgcgg ccacgacggc ttcatcggtc
     8401 atggggcgct cgccgtcgag accgatctcg gccgcgacat ccacgccacc gagagcggag
     8461 atgatcacgt cggcgccgga ctccttgccg aacaggtagt agatgcccga ggtgccgcgc
     8521 agatagagga acatcatgcg cagcggctta ccggatggca cgaccgaggc gatctcgctg
     8581 accttggcct cgatgccgct caccaggctc tgcgcaagca cctcgcccgc cgccggagcg
     8641 ccgagcgccg cggacacctg tcgcgccagc tcgcccaccc cgtcgatcga cggcgtcgtc
     8701 tcaacgaaga cgacggggat gcccgcctcc cgcagctgca gcacgacatc gatcgggccg
     8761 atgctgccgt cggtgatcac gagagtgggt cgcagagcga gaatcgactc gctgttgacc
     8821 gtgtgcccgc tcgaggtgat caccggcagg tcggcggcct ggctgaacgt cgtggagatg
     8881 tcgcgtccga ccatgctgtc gcccagcccg agaccgtaga cggtcgcggc gatcgagccc
     8941 gacaggtcca tggcgacgat ccggtcggtg gatgtcacgg tcaccggcac atcgccgtcg
     9001 aggtcgtgcg agctcaccgt cgcgggcaag gactgctcgg gcgcggtgtc gggagtgggg
     9061 atcgcgtcgg tgctcaggat cgccgtcgac tgccccacat acgagtgcgg gtcggtgagg
     9121 aaggtgagtt ccgtcagcgg tgcgctcggg tcagccgtca ccgcggagtc cacggtgccg
     9181 gtcggcgcag cagcgcagcc cgcgagggtc gctgcgatca gcgcgaagac ggcggcagtc
     9241 actgaggtgc gggtgcgcat gaggccaatc aggtcaagcg agggtagtaa ggcaatccta
     9301 agctctggcg tgtgggtgca tgctgagaaa gggcttcgcc cgcgttccct caccggatgc
     9361 tcacaccctc cgccctagga ttgccagcga caggaggcga cccgcgtgag tcaacccgag
     9421 agcagcgtgc ccgagcctgc agaccggcac gcgtcgggcc tgtcggtgcg ggatgcccgc
     9481 atcctcgact ttgagcgcca gtggtggcgt cacgcgggcg cgaaggagga ggcgatccgc
     9541 tccgagttct cactcagcgc ggcccgctat tatcagttgc tgaatgctgt gatcgattcg
     9601 cccgctgccg tgcgatacga cccgatgctg atcggacggc tgcagagggc acgggatgct
     9661 cgcacgtcgg cccgcgcagc acgcgcattc aggacctccg accggggcgg cgacagcctc
     9721 cacacagaag aatcgaacga ctgagccatg gcgagttacc ccaaagaccg gttcgaccag
     9781 ttgcccgaag accttcagcg catcggagcc caccgcggcc cggcgaagaa ggggcgcggc
     9841 tggatcggat tcgcttgggc cctcctggca accggcgtgc tcatcttcgg cggcctgttc
     9901 gccatcggca aggttctcgg catcgacctc ggcatcagca tcttcgcgcc ggcagcgacg
     9961 ccgaccccca cccccactcc gaccccgacg atggacccga tcctcgaccc cacgacgatc
    10021 gatcaggtcg cccgaggcat caagatcgac gtgctcaacg gcacacccat cgtcgatctc
    10081 gagaacaccg tcggagacaa gctcgcggct gctggctgga gaatgggcgc tttcaccgtc
    10141 gcatccgaca agacgcagga ggcgacgttc gtctactaca gcgaccccct caatgaggat
    10201 gtcgcccgag gcatcgtcgt ggcgatgggc atcggcgaga tccgcaaggt caccccggag
    10261 acgttccccg gcgcaagcat cacggtcgtg ctcggctccg actacccggt gcccaccgcg
    10321 gctccctgac gatccccagc ggccccacac gagcggtcga tcggcgcgaa atcgcgtgtt
    10381 gaatctcgat cgtgttacgc gcgtgtttaa tctcccgcat gtgcccattt cgtgaccaaa
    10441 ccgacctctt gtggggttga acgctccgct accatctggg attggcagcg ggctatcggc
    10501 tgtcgcgaca cagcagggag ttgcacatgg cgaacggaac cgtgaagtgg ttcaacgctg
    10561 aaaagggcta cggattcatc accgtggacg gcgggggaca ggacgtattc gttcactact
    10621 ccgccatcga catgaacggc tacaaggtgc tcgaagaggg ccaggcggtc atcttcgagg
    10681 tcggaaccgg tgcgaagggc ccccaggcgg agtcggtgaa gctggcctga ggccgcacgc
    10741 ccccgcacca cgccgcagtt cgtgcggcgt gcctctcagc tatgccctcc tttcttgcac
    10801 tcgcccccag cgagtgctag tttttccttt ggcactctca tgttcagagt gctaaagaat
    10861 tcgtctgcac gacgtccggg agggacgaga aacacacatg gcaaagatca ttgctttcaa
    10921 cgaagaggcc cgtcgtggcc ttgagcgtgg cctgaacatc ctggccgacg cggtcaaggt
    10981 gacgcttggc ccccgcggtc gcaacgtcgt tctcgagaag aagtggggcg cccccaccat
    11041 cacgaacgac ggtgtctcca tcgccaagga gatcgagctc gacgacccgt acgagaagat
    11101 cggcgccgag ctggtcaagg aggtcgccaa gaagactgac gacgtcgccg gtgacggtac
    11161 gaccacctcc gtggtgctcg cccaggccct cgtgcgcgaa ggcctccgca acgtcgccgc
    11221 aggcgccgac cccatcagcc tgaagaaggg catcgagaag gcggttgccg ccgtcatcga
    11281 ggcgcttgtc gccggtgcca aggagatcga gaccaaggaa gagatcgcgg ccaccgcatc
    11341 catctctgct ggtgactccg agatcggcgc cctgatcgcc gaggcgatcg acaaggtcgg
    11401 caaggagggt gttgtcaccg tcgaggagtc gaacaccttc ggcaccgagc tcgagctcac
    11461 cgagggcatg cgcttcgaca agggcttc
//
LOCUS       DAOSOU010000049        11233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5768_length_11233_cov_6.498121, whole genome shotgun sequence.
ACCESSION   DAOSOU010000049 DAOSOU010000000
VERSION     DAOSOU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5768_length_11233_cov_6.498121"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLW15_04260"
     CDS             <1..596
                     /locus_tag="PLW15_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT33646.1"
                     /translation="PEFRRGVTETADALGALGHTVTEVSFPYPANPLPLLARWFAGTE
                     TDAALLEARKLEPRIRTHARLGRLARRLGWLRQGPVDRIRAQVTAFFDDYDVLLTPTL
                     AQPGLEAVAWGQRGWYQNLMANIRYAPYPSLFNLLGWPAASIPAGWHATAEVPLAVQI
                     VVAPRADGAGEAAILGLAAQIERVRPWPRTVGVARLP"
     gene            666..1577
                     /locus_tag="PLW15_04265"
     CDS             666..1577
                     /locus_tag="PLW15_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT33647.1"
                     /translation="MSTAPARLHPLVLTAIIVTILAWASAFIVIRGTGPHFTGGALAL
                     GRLAVGAVLLGIVVLAGRRWVRPTAREWLLIVIYGVAWFGAYNVALNIAEHTLDAGTT
                     AMIVGVGPILIALGAGLFLGEGIPKWLAIGAAVAFVGVVLIGVGTGAAFGDGVGVFWA
                     IVSAVTYAIGVLAQKPAIKRLPNAQVTWLGATIGMVACLPFAGELITGLAEAPLPAIL
                     GVVYLGAVPTALAFSTWAYALSRMPAGQLGVTTYVVPPLVIIFGFLIFGEVPAVLAIA
                     GGVVCLVGVALSRRRARIPVSAETMAE"
     gene            1705..2244
                     /gene="rpsP"
                     /locus_tag="PLW15_04270"
     CDS             1705..2244
                     /gene="rpsP"
                     /locus_tag="PLW15_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT33648.1"
                     /translation="MAVKIRLKRLGKIRAPYYRIVVADSRSKRDGRSIEEIGKYHPTE
                     EPSVIEINSERAQYWLGVGAQPSPQVEALLKLTGDWGIFKGEKGAKSTVKFKEAKVEF
                     QADEKKKPVLKPKSEKPVAAPVEEAAAEPAAEVEETVVSDGAVEEAPAPEAADEAPAA
                     GDVVETPADEAAEAPADEA"
     gene            2248..2484
                     /locus_tag="PLW15_04275"
     CDS             2248..2484
                     /locus_tag="PLW15_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOT33649.1"
                     /translation="MLAPALEHLVKGIVDHPDQVQVVAKNSQRGEVLEVRVHPEDLGR
                     VIGRAGRTAKALRTLITALADGRRVRVDVVDTDF"
     gene            2488..3096
                     /gene="rimM"
                     /locus_tag="PLW15_04280"
     CDS             2488..3096
                     /gene="rimM"
                     /locus_tag="PLW15_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074994.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT33650.1"
                     /translation="MSAQKAPQPANTQLRVGRLTKAHGLKGAIKVELYTDAPERRFVP
                     GAVFALQVPVESEWHGKTIELSELKWYNSHPVAFFKNVSDRSAAESLIKAILWIDQDL
                     DEPSDEEDAWFDHQLVGLAVLRDGVQVGTVKQIDHLPAQDLLTIATSAGDVLVPFVKA
                     IVTSVDIEAGTMTVTPPLGLFEDLADDEATVEPEAGEPAPGE"
     gene            complement(3232..3738)
                     /locus_tag="PLW15_04285"
     CDS             complement(3232..3738)
                     /locus_tag="PLW15_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33651.1"
                     /translation="MLVRQVLLCCDRETAIVIIDSALNQRKLSLSALQEIVATVPSRF
                     SSLLDEVDAGSESGLETLCRLRLRGLGARIRSQVSIPGVGRVDLIIGERLVIEADGRE
                     WHEGRSAFHADRIRDLALLRLGYVVIRVSYPLIVTEWMLVELTVRALIGRREHLWSAT
                     HRREGLAR"
     gene            4299..4994
                     /gene="trmD"
                     /locus_tag="PLW15_04290"
     CDS             4299..4994
                     /gene="trmD"
                     /locus_tag="PLW15_04290"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT33652.1"
                     /translation="MRIDIVTIFPEFFGVLDVSLLGKARERGLIEVGVHDLRDFTHDK
                     HRTVDDTPYGGGAGMVMRPEPWGEALDAILADTTTVIFPSPAGERFTQATARELSLES
                     HLVFACGRYEGIDQRVVEYAASRAKVREISLGDYVLNGGEVAAMAMIEAVGRLVPGVI
                     GNPESLDEESHEGGLLEYPSYTKPAEWRGLAVPPILTSGNHGAIAAWRHEQQLERTAR
                     VRPDLLPDPEVTP"
     gene            4991..5440
                     /locus_tag="PLW15_04295"
     CDS             4991..5440
                     /locus_tag="PLW15_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33653.1"
                     /translation="MKYWYWSQPAVAVVLLAFTVYTLVSFFAFDASGLFSIQFAVNGA
                     FIFPGLLISLGLSQVLLSRRTPRSVTLPERILLGFEYLLIAVLIATSFEPDSLTVGLV
                     LWPLVIVLAVVITIAVVVTTARLAKPPAPPEPPTTDAEIDDLLGDKA"
     gene            complement(5437..6201)
                     /gene="map"
                     /locus_tag="PLW15_04300"
     CDS             complement(5437..6201)
                     /gene="map"
                     /locus_tag="PLW15_04300"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT33654.1"
                     /translation="MIELRTPAEIDRLRIAGEFVGSVLTATAAASAVGVNLLELDALA
                     HDMIRARGAESCYIDYHPSFGGSPFGKVICTSVNDAALHGLPHDYRLQDGDVLSLDFA
                     ASVDGWVSDSAVTIIVGTPRPEDQRLIEVTSRALDAGIAAATVGNRIGDISAAIGDVA
                     HAAGLKVNLDFGGHGVGTIMHGDPHIANDGRAGRGLPLRAGLVFAIEPWFMLGTKKLY
                     TDEDGWTLRSADGSRAAHFEHTVAVTDDGPVVLSAR"
     gene            complement(6198..6836)
                     /locus_tag="PLW15_04305"
     CDS             complement(6198..6836)
                     /locus_tag="PLW15_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT33655.1"
                     /translation="MNTKADFPVPTLAVRTEPVQQRSTERITSLLDAAAHLIDQNGID
                     GLTTSDVATRSGSSVGVVYRYFPNIQSLLRALAMRNLERFIDRIKNVGEGAEWPIAAD
                     AIIDGYIDLARTEPGFRAVRFGEVIDERFIHQPKEGTTTSIAHMVDNLLVSRYGFAAS
                     DGLSFDIEVLVELGDALLHRAFLYEASGDDRFIAKLRSLVRDYLTPYEVSAR"
     gene            7065..7415
                     /gene="rplS"
                     /locus_tag="PLW15_04310"
     CDS             7065..7415
                     /gene="rplS"
                     /locus_tag="PLW15_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT33656.1"
                     /translation="MHILDSVDAASLKDNIPDFRAGDNVKVHVNIIEGTRSRIQVFQG
                     VVIGRSGEGVRETFTVRKVSFQVGVERTFPVHSPIIDHIEIVTRGDVRRAKLYYLRGL
                     RGKKAKIKEKRDFS"
     gene            7491..8225
                     /gene="lepB"
                     /locus_tag="PLW15_04315"
     CDS             7491..8225
                     /gene="lepB"
                     /locus_tag="PLW15_04315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT33657.1"
                     /translation="MTTDSGTVPASDTSKPVRKGRGWKIFVRDILIIFVAALLISFLI
                     KTFLIRSFYIPSSSMEQTLHIDDRIIVNQLTPNLMPLEHGDVVVFKDPGGWLTPQPDP
                     QVNWFVGAVDAVLAFVGLSAPDSNDHLIKRVIGMPGDEVICCNDFGQLTVNGVPLDEP
                     YTTLPDGVTKATRDDFDVTVPEGSLWVMGDNRYNSADSAFHRNDPTGGFVPIENVVGR
                     ALLISWPVSRWTWLDNYPVTFAGVDG"
     gene            8229..8876
                     /locus_tag="PLW15_04320"
     CDS             8229..8876
                     /locus_tag="PLW15_04320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT33658.1"
                     /translation="MAVADPTLAVERALHRDGARFVIGCDEVGRGAIAGPVAVGLSLV
                     AAGVGEHPAGLRDSKLLSEKRREELSPLATDWSLHSAVGLATAEEVDTVGIMAALGLA
                     GLRALTQLHEQGARIHESVVLLDGAHDWLTPALSKPPRVQTQVKADRDCASVAAASVI
                     AKVHRDRIMRELDAVFPGYGWAGNKGYGSTEHFAAIDALGPSDVHRRTWLHPKAV"
     gene            8957..9280
                     /locus_tag="PLW15_04325"
     CDS             8957..9280
                     /locus_tag="PLW15_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HOT33659.1"
                     /translation="MDEDEFEDYDREVELALYKEYRDVVGQFRFVVETERRFYLANEV
                     ELVRQDTEHDFYFELTMNDVWVWDVYRSDRFVKSVRVLTFKDVNVEELSSKELELPKE
                     LALDE"
     gene            complement(9281..10153)
                     /locus_tag="PLW15_04330"
     CDS             complement(9281..10153)
                     /locus_tag="PLW15_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOT33660.1"
                     /translation="MSLDLAQLQALAAAVDTGTFDAAAGRLHVTPSAISQRIRALENS
                     VGSILLTRSKPVRVTEAGATYLALARKIDALTLDALAAQGSNQPLSLPIAVNGDSLAT
                     WVLPALAPLAGDIRFTFHREDQDHSTALLRDGTVMAAITTEATPVQGCTARPLGTMRY
                     RLLASPDFAARWFASGADATTIARAPVVVFDAKDDLQHRYVRDRWDTTIERHPHYVPS
                     SNDFVAAVAQGFGWATVPDLQSAPLLARGALVELDPGHHLDVALHWQQWALSTSALDR
                     TAAAIAEAARAALT"
     gene            10223..10846
                     /locus_tag="PLW15_04335"
     CDS             10223..10846
                     /locus_tag="PLW15_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HOT33661.1"
                     /translation="MPHAFAAALAGLGLGLSLIVAIGAQNAFVLRQGLRREHVLIVVV
                     VCAASDAILITAGVAGLGVLIQQAGWILVVVRIAGAAFLTWYGIGAARRAIKPKVLEA
                     DEGGDRMTRAAAISTVLALTWLNPHVYLDTVVLLGSIGGTYGDARWWFALGATIGSIV
                     WFTALGFGSRALRPLFAKPVAWRVLDALIAVVMFAIAISLVVGLFTE"
     gene            complement(10860..>11233)
                     /locus_tag="PLW15_04340"
     CDS             complement(10860..>11233)
                     /locus_tag="PLW15_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33662.1"
                     /translation="RSGAGVLAWLAVVAILALFTLALTWLAVIAGLSAKSVDGASAFS
                     YPLIFLPFISSAFVPTDSMPGPVRWFAENQPVTSIVNSIRHLLMGQSVGIDLWIALAW
                     CGGILVVAFILAMVVYRRRIS"
BASE COUNT         1798 a         3754 c         3816 g         1865 t
ORIGIN      
        1 accccgagtt ccgtaggggc gtcaccgaga ccgccgacgc gctgggggcg ctcggccaca
       61 cggtcaccga ggtgtccttc ccctacccgg ccaacccgct gccgctactc gcgcggtggt
      121 tcgcgggcac cgagacggat gccgcactgc tcgaggcgcg caaactcgag ccgcgcatcc
      181 gcacgcacgc ccggctcggg cgactcgcgc gcaggctcgg ctggctcagg caggggccgg
      241 tggatcgcat ccgcgcccag gtcaccgcct tcttcgacga ctacgacgtg ctgctcacgc
      301 cgacgctggc ccagcccggg ctcgaggccg tggcgtgggg tcagcgcggg tggtaccaga
      361 acctcatggc caacatccgg tacgcgccgt acccgtcgct gttcaatctg ctcggctggc
      421 ccgccgcgag catccccgcg ggatggcacg cgaccgcaga ggttcccctc gcggtgcaga
      481 ttgtggtcgc accccgggcc gacggagcgg gcgaggcggc gatcctcggg ctggccgctc
      541 agatcgaacg cgtgcgcccc tggccgcgca ccgttggggt cgcccgtctg ccatagttcg
      601 gcgggtttcg gtcatcgcgc cgcacgaccg gtcgggatgg cggtggctcg cagtagcgtg
      661 aacccatgag caccgccccc gcacgtctgc atccgcttgt tctgacggcg atcatcgtca
      721 cgatcctcgc gtgggccagc gccttcatcg tcatccgggg caccgggccg cacttcacgg
      781 gcggagcgtt ggccctcggc cgcctcgcag tgggtgcggt tctgctgggg atcgtcgtgc
      841 tggccgggcg ccgctgggtg cgcccgaccg cgcgcgagtg gctgctcatc gtcatctacg
      901 gcgtggcgtg gttcggcgcc tacaacgtgg cactcaacat cgcggagcac accctcgacg
      961 ccggcaccac ggccatgatc gtcggggtcg ggcctattct gatcgcgctc ggtgcagggc
     1021 tgttcctcgg ggagggcatc ccgaagtggc tggcgatcgg cgctgccgtg gccttcgtgg
     1081 gcgtggtgct catcggggtg ggcaccggcg cggccttcgg cgacggagtc ggggtgttct
     1141 gggcgatcgt gtcggcggtg acctacgcga tcggagttct cgcgcagaag cccgcgatca
     1201 agcgcctgcc caacgctcag gtcacctggc tgggtgcgac gatcggcatg gtcgcctgcc
     1261 tgccgttcgc cggtgagctg atcaccgggc tcgcggaggc cccgctgccc gcgatcctcg
     1321 gcgttgtcta tctgggcgcc gtgcccaccg ccttggcgtt cagcacgtgg gcctacgccc
     1381 tgtcccggat gcccgccggc cagctcggcg tcaccaccta cgtcgtgccc ccgctcgtca
     1441 tcatcttcgg cttcctcatc ttcggcgagg tgcccgccgt gctggcgatt gccggcggtg
     1501 tcgtgtgtct cgtcggcgtc gcgctgtccc gccgcagagc gcggattccc gtcagcgccg
     1561 aaaccatggc agaatagcca gtcgagtcct tcccgtccga ccctctaatc agacgcgagt
     1621 ggaatccatc agagctttcg tgccgagtgt gcccccacgc ccacggcttg cggttcgcca
     1681 ccaacacatg tactcaggag aattgtggct gtaaagattc gtttgaagcg tctcggcaag
     1741 atccgtgcgc cctactaccg catcgtcgtc gccgactcgc gcagcaagcg cgatggtcgc
     1801 tcgatcgagg agatcggcaa gtaccacccg accgaggagc cctcggtcat cgagatcaac
     1861 tcggagcgcg cgcagtactg gctcggcgtg ggcgcacagc cctccccgca ggtcgaggcg
     1921 ctgctcaagc tcacgggcga ctggggcatc ttcaagggcg agaagggtgc caagagcacc
     1981 gtcaagttca aggaagccaa ggtcgagttc caggctgacg agaagaagaa gcccgtgctc
     2041 aagcccaaga gcgagaagcc cgttgccgcc ccggtcgagg aggcagccgc cgagcccgcc
     2101 gccgaggtcg aggagaccgt cgtgagcgac ggcgccgtcg aagaggctcc cgctcccgag
     2161 gctgccgacg aggctcccgc cgccggtgac gtcgtcgaga cgcccgccga cgaggccgcc
     2221 gaggctcccg cggacgaggc ctaggtcttg ctcgctcctg ctctcgaaca cctggtcaag
     2281 gggatcgtcg atcatcctga ccaggttcag gtcgtggcga agaattcaca gcgcggcgag
     2341 gttctcgagg tgcgcgtgca ccccgaggac ctcggtcgcg tcatcggtcg tgccggtcgc
     2401 acggcgaagg cgcttcgcac gctgatcacg gctctcgccg acggtcgccg cgtgcgtgtc
     2461 gacgtcgtcg acaccgactt ctaggctgtg tccgcccaga aagcccccca gccggccaac
     2521 acgcagttgc gggtcggcag gctcaccaag gcacacgggc tgaagggcgc catcaaggtc
     2581 gagctgtaca cggatgcccc ggagcgacgt ttcgttccgg gcgccgtgtt cgcgctccag
     2641 gtgccggtgg agtccgagtg gcacggcaag accatcgagc tgtccgagct caagtggtac
     2701 aacagccatc cggtcgcgtt cttcaagaac gtgtcggatc gcagcgcagc cgaatcgctg
     2761 atcaaggcga tcctctggat cgaccaggat ctcgatgagc cctccgacga ggaggatgcc
     2821 tggttcgacc accagctcgt gggtcttgcc gtactgcgtg acggagtcca ggtgggcacc
     2881 gtcaagcaga tcgaccacct gcctgcccag gacctgctga cgatcgccac gagcgccggt
     2941 gacgtgctgg ttccgttcgt gaaggcgatc gtgacatccg tcgacatcga ggcgggaacg
     3001 atgacggtga ccccaccgct gggcctcttc gaggacctcg ccgacgacga ggcgacggtc
     3061 gagcccgagg cgggcgagcc cgcccccggc gagtagctcg cacccggccc gctgctgcac
     3121 gcacgtcgca cgcgcgttgc tgcacgcacg ccggcccgcg cgttgcggag gatggcgcgc
     3181 cgtcagactt ccgcgtgcgg cctccgttcg gcgcgccatc ctccgcaacg gtcaccgggc
     3241 gagtccctcg cgccggtgcg tcgcgctcca caggtgctcc cgccgaccaa tcagcgcgcg
     3301 gactgtgagc tccacgagca tccattcggt cacgatgagc gggtagctca ccctgatcac
     3361 gacgtagccg aggcgcagta gcgccagatc acggatgcga tcggcgtgaa aagcgctcct
     3421 gccctcgtgc cactcccgac cgtcggcctc gatgacgagc cgctccccga tgatgagatc
     3481 gacccgaccc acgcccggga tgctcacctg cgatcggatt cgggcaccga gacctcggag
     3541 gcgaagccga cacagagtct ccagccccga ttcgctaccc gcatccacct cgtcgagcaa
     3601 ggacgagaac ctgctcggca cggtagcgac gatctcctgc agcgcgctca agctcagctt
     3661 gcgctggttg agcgcggagt cgatgatgac gatcgctgtt tcgcggtcgc agcacagaag
     3721 cacctgtcgc acgagcactt tgacagattc aaccgtcgcg ggctcgtcgc gccagttggc
     3781 cgagacccaa tggaccgtgc acgtagcgga atcgctgaat cggcggccgg catgccgggg
     3841 gagcgcgata tgcaactcct ctgtggtctc cggcacccat aggccgtggt gtcgcgccgc
     3901 ggagaggcaa gccagccgac caccgagccg cacggcgcgc accacatcgg cgggcgcatc
     3961 cgtcaccgtg acccagccct tgcgtagtcg ccgcacatca ccacgggcaa ccgccgaccg
     4021 tatctgtgcg gccgtgtagc ccatccgtag aagcactgtg acacgcgcca ctccgccacg
     4081 gcggcggacc tcgttggtga ctgagcccat ggcatcagga tgcgcgtcag gggcatcgaa
     4141 ggatcaccgg ccgtacgtcc cgtggaaacc gcgggcaagc gccagggtgt ggaggatggc
     4201 acgccgtagc ggggactcgg caggtagcac ctacggcgtg tcgtcctccg caacggtgcg
     4261 agggccttcc ccgcgtgcca cccgggtagg gtcgactcgt gcgcatcgac atcgtcacga
     4321 tctttcccga gttcttcggg gtgctcgacg tgtccctgct cggcaaggcg cgcgagcgcg
     4381 ggctgatcga ggtgggagtg cacgacctgc gcgacttcac ccacgacaag catcgaacgg
     4441 tggatgacac gccctacggc ggcggcgccg gcatggtcat gcggccggag ccgtggggcg
     4501 aggccctcga cgcgattctg gcggacacca ccacggtgat cttcccgtcg ccggcggggg
     4561 agcgcttcac ccaggcgacg gcgcgcgagc tgagtctcga gtcgcacctc gtgttcgcct
     4621 gcgggcggta cgagggcatc gaccagcggg tggtcgagta cgcggcatcc cgggcgaagg
     4681 tgcgtgagat cagcttgggc gactacgtgc tcaacggcgg tgaggtcgcc gccatggcga
     4741 tgatcgaagc cgtcgggcgc ctcgttccgg gcgtgatcgg caatccggag agcctcgacg
     4801 aggagagcca cgagggcggt ctgttggagt atccgagcta caccaagccc gccgagtggc
     4861 gcggcctcgc ggtgccgccg attctgacga gtggcaacca cggcgcgatc gcggcctggc
     4921 ggcacgagca acagctcgag cgcactgcgc gggtgcgccc cgatctgctc cccgacccgg
     4981 aggtcacccc gtgaagtatt ggtactggtc gcagcccgcc gtcgcggtgg tgctgctcgc
     5041 attcaccgtc tacaccttgg tgagcttctt cgcgtttgac gcgtcgggcc tgttctcgat
     5101 ccagttcgcg gtgaatggag ccttcatctt cccgggcctg ctgatctcgc tcggcctgag
     5161 tcaggtgctg ctgtctcgcc gcacaccgcg ttcggtgacg ctcccggagc gcatcctgct
     5221 ggggttcgag tacctgctga tcgcagtgct catcgcgacg tcgttcgagc cggactcgct
     5281 gaccgtcggg ttggtgctct ggccgctggt gattgtgctc gcggtcgtga tcacgatcgc
     5341 tgtcgtcgtc acgacggcac gtctggcgaa accaccggcc ccgccggaac ccccgacgac
     5401 ggacgccgag atcgacgacc tgctgggcga caaggcctag cgagccgaga gcactaccgg
     5461 gccgtcgtcg gttacggcga cggtgtgctc gaagtgcgct gcacgtgacc cgtcggcgct
     5521 gcgcagagtc cagccgtcct catcggtgta cagcttcttc gtgccgagca tgaaccacgg
     5581 ctcgatggcg aagacgagtc cggcgcgaag gggaagcccg cgaccagccc gcccgtcgtt
     5641 ggcgatgtgc gggtcgccgt gcatgatggt gcccacgcca tgcccgccga agtcgaggtt
     5701 caccttgagt ccggccgcgt gcgcgacatc gccgatggcg gccgagatgt cgccgatccg
     5761 gttgccgacg gtcgccgcag cgatgcccgc gtcgagtgcg cgcgaggtga cctcgatcag
     5821 gcgctggtcc tcggggcgcg gggtgcccac gatgatcgtc accgccgagt cgctcaccca
     5881 gccgtcgacg gatgcggcga agtcgagcga gagcacgtcg ccgtcctgca gacggtagtc
     5941 atgcgggagg ccgtgcaggg ccgcgtcgtt gacggaggtg cagatcacct tgccgaacgg
     6001 cgagccgccg aacgaggggt ggtagtcgat gtagcagctc tcggcaccgc gggctcggat
     6061 catgtcgtgg gcgagagcat cgagctccag gaggttgacg cccactgcgg acgccgcggc
     6121 ggttgccgtg agcacgctgc cgacgaattc gccggcgatg cgcagtcgat cgatctccgc
     6181 gggtgtccgc agctcgatca ccgggcggag acctcgtagg gcgtgaggta gtcacgcacc
     6241 agcgagcgga gcttggcgat gaaacggtca tcgccggatg cctcgtacag gaacgcacgg
     6301 tgcagcagcg cgtcacccag ctcgacgagc acctcgatgt cgaaggacag accgtcggat
     6361 gcggcgaagc cgtagcggga gaccagaagg ttgtcgacca tgtgggcgat cgacgtcgtc
     6421 gtgccctcct tcggctggtg gatgaagcgc tcgtcgatga cttcgccgaa gcgcaccgca
     6481 cggaaacccg gctcggtacg tgcgaggtcg atgtagccgt cgatgatggc gtcggcggcg
     6541 atgggccact cggcgccctc tccgacattc ttgatgcggt cgatgaagcg ttccaggttg
     6601 cgcatcgcaa gggcgcgcag cagcgactgg atgttcggga agtagcggta gacgacgccg
     6661 accgacgatc cggagcgggt cgcgacgtcg ctggtggtga ggccgtcgat gccgttctgg
     6721 tcgatcaggt gagccgcggc atccagcagg ctggtgatgc gctcggtgct gcgctgctgc
     6781 acgggctcgg ttcggacggc gagagtgggg acaggaaagt ccgccttggt gttcattgtg
     6841 gtgaaagctc ctcgttggga atgactgtct tcgacgatac cccgagcgcc tctcagtaag
     6901 ctgagtggca cggcgagccg gttatatgcg agaattgccc ttcgtgccag tcaccggatc
     6961 tgccacaggg gttccggggt tctgactaac ggcgcacatc cattcttcct gaagtacatc
     7021 aacagcgcgt tcgacctgtg agcgggcgca gagagcgaat caccatgcat atcctcgaca
     7081 gcgtcgacgc agcatccctc aaggacaaca tccccgactt ccgcgccggc gacaacgtca
     7141 aggtgcacgt gaacatcatc gaaggcacgc gctcgcgtat ccaggtcttc cagggtgtcg
     7201 tcatcggccg ctcgggtgag ggcgtgcgcg agaccttcac cgtgcgcaag gtgagcttcc
     7261 aggtcggcgt cgagcgcacg ttccccgtgc actccccgat catcgatcac atcgagatcg
     7321 tcacccgcgg tgacgttcgt cgcgccaagc tctactacct gcgcggtctg cgcggcaaga
     7381 aggcgaagat caaggagaag cgcgacttct cgtaacgagg tttcgcccag cgcgtttcat
     7441 cgaatcctga gctcccagac gcatatgctt gctgggagct caggggttta atgactacgg
     7501 attcaggcac agtgccagcc agcgacacat ccaagcccgt ccgcaaggga cgcggctgga
     7561 agatcttcgt gcgcgacatc ctcatcattt tcgtcgcagc cctgctgatc tcgttcctca
     7621 tcaagacgtt cctgatccgc tcgttctaca tcccgtcgtc gtcgatggag cagaccctgc
     7681 acatcgacga ccgcatcatc gtgaaccagc tcacccccaa cctgatgccg ctcgagcacg
     7741 gcgacgtcgt ggtgttcaaa gacccgggcg gctggctgac gccgcagccc gacccccagg
     7801 tcaattggtt cgtcggggct gtggatgccg tgctcgcctt cgtgggcctg agcgcccccg
     7861 acagcaacga tcacctcatc aagcgtgtca tcgggatgcc cggcgacgag gtcatctgct
     7921 gcaacgactt cggtcagctc accgtcaacg gcgtgccgct cgacgagccg tacacgaccc
     7981 tgcccgacgg cgtcaccaag gcgacccgcg acgacttcga tgtcacggtg cccgagggat
     8041 cgctctgggt gatgggcgac aaccgctaca actccgccga ctcggcgttc caccgcaacg
     8101 acccgaccgg cggtttcgtg ccgatcgaga acgtcgtcgg tcgggcgctg ctgatcagct
     8161 ggccggtcag tcgctggacc tggctcgaca actatcccgt cacattcgcg ggcgtggacg
     8221 gctagttcgt ggctgtcgcc gaccccaccc tcgccgtcga gcgtgccctg caccgtgatg
     8281 gcgcccgttt cgtgatcgga tgcgacgagg tcggccgcgg agcgatcgcc ggcccggtcg
     8341 cggtggggct gtcgctcgtg gccgccggag tcggcgagca ccccgccgga ctgcgggact
     8401 cgaagctgct cagtgagaag cgccgcgagg agctctcccc gctagcgacg gactggtcgc
     8461 tgcactcagc cgtgggtctc gccacagccg aggaggtcga cacggtcggc atcatggccg
     8521 cgttgggtct ggccggtctg cgcgcgctga ctcagctgca cgagcagggc gcccggatcc
     8581 acgagagtgt ggtgctgctc gacggagccc acgattggct gacgcccgcg ctgtccaagc
     8641 ccccgagggt gcagacccag gtgaaggccg accgcgattg cgcttcggtg gctgcggcat
     8701 ccgtcatcgc caaggtgcac cgcgaccgca tcatgcgcga gctggatgcc gtgttccccg
     8761 gctacggctg ggccggcaac aagggctacg gctcgactga gcacttcgcg gcgatcgacg
     8821 ccctcggccc gtccgacgtg catcggcgca cctggctgca cccgaaggct gtttgagtag
     8881 gtccgtaggg ccgtatcgaa accaacgaga actggtggtc gagcgtgtcg agacccgaac
     8941 tagactctta accagcatgg atgaggacga gttcgaagac tacgaccgcg aggtcgagct
     9001 ggcgctgtac aaggagtacc gcgatgtggt gggccagttc cgcttcgtcg tggagaccga
     9061 gcgccgcttc tacctggcca acgaggtcga actcgtgcgc caggacaccg agcacgactt
     9121 ctacttcgag ctcacgatga acgatgtctg ggtgtgggac gtctaccgtt ccgaccgctt
     9181 cgtgaagagc gtgcgcgtgc tgacgttcaa ggacgtcaac gtggaggagc tctcctccaa
     9241 ggagctcgag cttcccaagg aactcgcgct cgacgagtag ctaggtcagg gcagcccgag
     9301 ctgcttctgc gattgccgcc gccgtgcggt ccaacgccga ggtgctgagc gcccactgct
     9361 gccagtgcag cgccacgtcg agatgatgtc cggggtcgag ctccacgagc gcgccgcggg
     9421 ccagcagcgg tgccgactgc aggtcgggca cggtcgccca gccgaagccc tgcgccacgg
     9481 ccgcaacgaa gtcattcgag ctgggcacgt agtgcggatg acgctcgatc gtcgtgtccc
     9541 accggtcgcg cacatagcga tgctgaaggt cgtctttggc gtcgaacacc accaccggcg
     9601 cacgggcgat ggtcgtggca tccgccccgc ttgcgaacca gcgcgcggcg aagtccggcg
     9661 aggccagcag acggtagcgc atggtgccga gcgggcgcgc cgtgcagccc tgcacgggcg
     9721 tggcctcggt cgtgatggcc gccatcaccg tgccgtcgcg caggagggcg gtcgagtggt
     9781 cctgatcctc gcggtggaag gtgaagcgga tgtcgccggc cagcggcgcg agggcgggca
     9841 gtacccaggt ggccagtgag tcgccgttga cggcgatcgg caacgacagt ggctggttcg
     9901 atccttgggc cgccagtgcg tcgagggtga gcgcgtcgat cttgcgggcg agcgcgagat
     9961 aggtcgcgcc ggcctcggtg acccgcaccg gcttcgaccg tgtcagaagg atgctgccga
    10021 ccgagttctc gagcgcccgg atgcgctggc tgatcgccga cggggtgacg tggagtcgtc
    10081 cggcggccgc gtcgaaggtt cccgtgtcga cggcggcggc cagtgcctga agttgggcga
    10141 gatcgaggga cacctcagct attctaatgt tccgtaagaa tcattcgctg cactgatgga
    10201 gcgtgtgccc tagcgtgaac gggtgcccca cgctttcgcc gccgctctcg ccggcctcgg
    10261 actcggtctc tcgctcatcg tcgcgatcgg cgcgcagaac gcgttcgtgc tgcggcaagg
    10321 ccttcgccgc gagcacgtgc tcatcgtcgt ggtcgtgtgc gcggcatccg atgcgattct
    10381 catcacggcg ggggtcgccg gcctcggcgt gctcatccag caggccggtt ggatcctcgt
    10441 ggtcgttcgc atcgcgggtg cggccttcct cacctggtac ggaatcggtg ccgcccgccg
    10501 ggcgatcaag cccaaggtgc tcgaggccga cgagggcggc gaccggatga cgcgagccgc
    10561 cgccatctcg acggtgctcg cgctcacctg gctcaacccg cacgtgtacc tcgacaccgt
    10621 cgtgctcctg ggatcgatcg gcggaaccta cggcgacgcc cgctggtggt tcgcgctcgg
    10681 cgcgactatc ggcagcatcg tctggttcac cgccctcggc ttcggcagcc gcgccctgcg
    10741 cccgctgttc gcgaagccgg tcgcctggcg cgtgctcgat gcgctcatcg cggtggtgat
    10801 gttcgccatc gcgataagcc tcgtcgtcgg gctgttcacc gagtaggtcg gacagacggt
    10861 cagctgatcc ggcggcggta gacgaccatc gcgaggatga atgcgaccac caggattccg
    10921 ccgcaccatg ccagcgcgat ccacaggtcg atgcccaccg actgccccat cagcaggtgt
    10981 ctgatcgagt tgacgatgga cgtcaccggt tggttctcgg cgaaccaccg caccggaccc
    11041 ggcatgctgt ccgtgggcac gaatgccgag ctgatgaagg gcaggaagat cagggggtag
    11101 gagaacgcgc tggcgccatc caccgacttg gcggagagcc cggcgatcac cgcgagccac
    11161 gtcagggcga gggtgaagag ggccaggatc gcgaccactg cgagccacgc cagcacacca
    11221 gcccccgacc gga
//
LOCUS       DAOSOU010000050        10981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5949_length_10981_cov_6.708860, whole genome shotgun sequence.
ACCESSION   DAOSOU010000050 DAOSOU010000000
VERSION     DAOSOU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5949_length_10981_cov_6.708860"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1412
                     /locus_tag="PLW15_04345"
     CDS             3..1412
                     /locus_tag="PLW15_04345"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT33663.1"
                     /translation="MAEISTSSITGTTSSITGSTTLIDPSTGQAFETVHQTSREQVDD
                     AIASAVVAQRAWAALAPAARAQALRDFATVVGTHVEELAALEVVNSGHPISQARWEAG
                     HVRDVLNYYAAAPERMIGKQIPVAGGLDVTFLEPLGVVGVIVPWNFPMTIASWGFAPA
                     LAAGNAVVLKPAEWTPLTAIRLGELALEAGLPDGLFQVLPGKGSVVGNRFVTHEQVRK
                     IVFTGSTEVGKQVMAGCADQVKAVTLELGGKSANVVFADSDLEKAAATAPYAVFENAG
                     QDCCARSRILVERSVYDRFMELLEPAVKGVVVGAPGDPATEMGPLVSKAHFDAVSAYV
                     PDDAAVAFRGSKPDGPGYWFAPTVLTPSSRTDPVVTEEIFGPVVAVLPFDDEADAIAL
                     ANSSIYGLSGSIWTRDVGRAIRVARGVESGNLSVNSHSSVRYSTPFGGFKQSGLGREL
                     GPDAASAFTETKNVFISTD"
     gene            1499..2287
                     /locus_tag="PLW15_04350"
     CDS             1499..2287
                     /locus_tag="PLW15_04350"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HOT33664.1"
                     /translation="MTITKIDLTQRLKGKVAVITGGASGIGFATARRFAAEGATVVIG
                     DFNAETGEKAAAEVGGLFVQVDVTDEEQVNHLFDTAAEKYGSVDIAFNNAGISPPDDD
                     SIETTELPAWNRVQDVNLKSVYLCSRAALRHMVKQQSGSIINTASFVAVMGSATSQIS
                     YTASKGGVLAMTKELGVQFARQGIRVNALCPGPVNTPLLQELFAKDPERAQRRLVHIP
                     KGRFAEPEELAAAVAFLASDDASFITASTFLVDGGISSAYVTPL"
     gene            2290..3024
                     /locus_tag="PLW15_04355"
     CDS             2290..3024
                     /locus_tag="PLW15_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT33665.1"
                     /translation="MATPGEDATYEGLADEVLLRPVRSGNSFEDTVARLLQTIRLGVV
                     GPGEPLPPERDLAARFSVSRDTVRDAIKSLADAGYLVSRRGRYGGTFVSEPLPTSRAD
                     TPPPAALDLNDVLGLREILEVGAARAAAARELSGVQRDLLWTRLRETAAASVTDYRRL
                     DSRLHLTIAEVVGTPSLVSLLADNRTRVNELLDGIPLLAKNIEHSNQQHEDIVVAILT
                     GNPERAALAMGEHLEGSAALLRGFLS"
     gene            complement(3026..3463)
                     /locus_tag="PLW15_04360"
     CDS             complement(3026..3463)
                     /locus_tag="PLW15_04360"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOT33666.1"
                     /translation="MSVTFDTVLTGGGTASGIVVPEEIMAQLGPAKRYPVVVTVGDYS
                     YRSSVSWYKGAFMISVSSAVKAEAGLTGGDAITVTLEHDTEPRVLELPAPVADALEAA
                     DALEAFRALSYSKQRAVVEPWVAAKSDATRDKNLEKILAAARG"
     gene            complement(3470..3748)
                     /locus_tag="PLW15_04365"
     CDS             complement(3470..3748)
                     /locus_tag="PLW15_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33667.1"
                     /translation="MNFDGPGPFAPFAHFAMMIVGGLLWLFMLLVVIALIFLLVRFLL
                     VATKAAQIYVAKNSPAVSSEAPNTASAAPATTTPPAKAAPRPRTPKAP"
     gene            complement(3745..3939)
                     /locus_tag="PLW15_04370"
     CDS             complement(3745..3939)
                     /locus_tag="PLW15_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33668.1"
                     /translation="MRRFLFNTSILSALFGVFGVVQSTRRGPRDWRLILMWISWGLTV
                     AIAVGTVVKDAQTNELEEGP"
     gene            4008..4673
                     /locus_tag="PLW15_04375"
     CDS             4008..4673
                     /locus_tag="PLW15_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOT33669.1"
                     /translation="MADVVNGFTPRAFAFFRELAANNTREFWLAHKAEFEAVVRDPME
                     SLLAALPEPYDGFRPFRMNRDVRFSKDKSPYKTAHAAVLDDRGAVLYLQVGAEGMFAA
                     GGGYLLAPDQLERFRAAVDAPAPGLELERIIDELGRDGIDVGPGGAEPLKTAPRGYDK
                     EHPRIGLLRQKGIAAGIRLDPEDCRNGDELLATVVDFFDAVQPLTSWLRDHVGPSTAP
                     RGR"
     gene            complement(4684..5529)
                     /locus_tag="PLW15_04380"
     CDS             complement(4684..5529)
                     /locus_tag="PLW15_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOT33670.1"
                     /translation="MRRSTVSSFSACIATANRRKDYPVISAMFDKERSHRLLEALGAP
                     LPRDFRVIGELSRLTPDHLVEPTVLKPARSYNNRGVVALVPLGGHRWFEQITATELTF
                     AELTARLDAEREKAGFADRWIGEELLRAADGSDRPVDDVKISMFGDYMACSFVRSTRP
                     RGFQWFDDVWEKVNVGIHTEKLDPTLNAPENAEQLLGIARAIGAALPLPYLRVDLYPT
                     DRGPVVGELTPLPGWYFNFNDEWDERMGRLYEERERYLFSRGMDWSRVTPPDIHTILA
                     DLGLT"
     gene            5627..6181
                     /locus_tag="PLW15_04385"
     CDS             5627..6181
                     /locus_tag="PLW15_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT33671.1"
                     /translation="MSSQEDLRTIALAEFATAGYAGTSLQRIAELAGLSKSSVLYHYA
                     SKEALLEAAIGPAIDRMIAILEPLVGLELDDDARTTFLEAFVDFLLDHRLEVHMFINQ
                     GPSLVDVPVIDRANALVFRLAEFFSANSASLEEKMRFGIALGGAAYMLCTVHSLDLPV
                     EPREETRVALLTIMGELLAPVRIR"
     gene            6257..>7167
                     /locus_tag="PLW15_04390"
     CDS             6257..>7167
                     /locus_tag="PLW15_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT33672.1"
                     /translation="MATLLYRLGRFSYRHAWRVIGVWVLVLIAVLGAGIGLGGKTQES
                     FAIPGTESQQALDRLEAVFPSVAGASATAVVVAPAGASIGSEKATIEELVTAIEKVPG
                     IDSAMSPFDQYAGKAISDDGTMAIIRVQFDGPSTDVTDATIADLKATAEVGTAEGLQV
                     EFGGQVFQETSVAITITEVFGVIFAGVVLVITFGSLLAAGMPLLSALLGVGIVMGAIM
                     TLSAFTTVSSSAPLLALMIGLAVGIDYALFILSRHRTQLAKGEDPEESAAMAVGTAGS
                     AVVFAGVTVIIALLGLLVVGIPFLSVMG"
     assembly_gap    7168..7267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7268..9140
                     /locus_tag="PLW15_04395"
     CDS             <7268..9140
                     /locus_tag="PLW15_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT33673.1"
                     /translation="GEVSTSSTTERPHRTMGLRWVTGVMKRPVLATLGVITILGTLAI
                     PAMSLDLNLPDGGSQPVDSTQRKAYDLVSEGFGPGFNGPLIVAVDITQTVTIMEDLDS
                     IGSQLRGLADVAYVSQGFPDEGLDTAIIQVTPSSAPDSLETKQLVQTIRDLAPSIAAK
                     YDTPITVTGTTAIGIDISNRLTSALVPFGLIVVGLSIVLLMMVFRSVLVPIKAALGFL
                     LSVVASFGVVVAVFQWGWLGDIIGVENPGPILSFMPIILMAVLFGLAMDYEVFLVSGM
                     REEFVKTGDARYAVKHGFANGARVVTAAALIMFFVFFAFVPEGSGMIKPIALGLAVGI
                     AFDAFLVRMTLVPALMTLFGKAAWWMPRWLGRLLPNVDIEGEQLREHKHAVDWARGEG
                     ELALSAEYLVAGSREHEVGPLSISVPRGAIVVASGDPLDRRLVAATLSGRLDPVSGRA
                     QVAGHPLPSEAADVRSLVALADIGGSQRSETSVTVGELLVERLEMTLPWYRLYRTRRA
                     ARRWLARINDVLGSAASTVRAESTLVELPQLERAVALAAVALAERTPVVMLDQLDPFA
                     NPEDEAAFIAAINALAPATTTLVVGTPLPGRATDVASNRPRIDIDLYSLSASEGFAQ"
     gene            9137..10807
                     /locus_tag="PLW15_04400"
     CDS             9137..10807
                     /locus_tag="PLW15_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HOT33674.1"
                     /translation="MTTSWLRRVGIAAVVLIPLAFAGLFVGAVGQGKSAIDSIPVAIV
                     NQDTLQKVTNADGSVTNVFAGRQLVTELTGSEGFDWTITNAADAEAKLASGEVYAVLT
                     VPSNFSDSILSLSSADPKKADISIRTDDTHSYLTGSVAQVVGQTMTDTFGKAITAQYI
                     GGIYSSLGDLGTSLTAAADGATSLANGATSLSSGLTQYTDGVGSLASGLSQLEDGAAQ
                     LSSGAATYTGTVSQISAGLTALAPAMSTADPALQAQFNALVAGLSQTAAGGSSLTTGV
                     AGVQSGISQSASGAWQLANSSDQLTSGASQLASGATDLATGLQSGADQLPSSNADTAA
                     ASAEVAADPVGLTVSTDNAVSQVGQIVATFFVPLGLWVGALAVFLVMRPVTRRALTST
                     ARNGRLVASAIGKASLITGAQAVLLVALLHGALGVAWSLLPATLGFSLLIAIAFTAFH
                     YLLTIGLGRAGLIVSLFLLAVQITSTGGIYPIELLSTPFQVVSPFLPLTYAVQGMQGI
                     LAGGNPGGVIAAAAVLAAFGIGSTVLALFAIRRTRRAGAIGLLPAVAS"
BASE COUNT         1683 a         3823 c         3525 g         1850 t
ORIGIN      
        1 ccatggctga gatctcgaca agctcgatca ccggaacgac aagctcgatc accgggtcga
       61 cgaccctcat cgacccctcg accggtcagg cattcgagac cgtgcaccag acctcacgcg
      121 agcaggtgga tgacgcgatc gcgagcgccg tcgttgcgca gcgcgcgtgg gcggctctcg
      181 ctccggcggc tcgcgcccag gccctccgcg acttcgcgac ggtcgtgggc actcacgttg
      241 aagagctcgc ggccctcgag gtggtcaact ccgggcatcc gatcagtcag gcccgttggg
      301 aggcggggca cgtgcgcgac gtgctcaact actacgcggc ggcgccggag cggatgatcg
      361 gcaagcagat ccccgtggcc ggcggcctcg acgtgacctt cctcgaaccg ctcggcgtcg
      421 tgggcgtgat cgtgccgtgg aacttcccca tgacgatcgc gagctggggc ttcgcgcctg
      481 cactcgccgc gggcaacgcc gtcgtgctga agccggccga gtggacgccg ctgaccgcga
      541 tccgcctcgg tgagctcgcg ctcgaggccg ggctgcccga cgggctgttc caggtgctgc
      601 cgggcaaggg ctcggtcgtc ggcaaccgct tcgtcaccca cgagcaggtg cgcaagatcg
      661 tcttcaccgg ctcgaccgag gtcggcaagc aggtcatggc cgggtgcgcc gaccaggtga
      721 aggccgtcac cctcgagctc ggcggcaaga gcgccaacgt ggtgttcgcc gactccgacc
      781 tggagaaggc cgcggccacg gcgccgtacg cggtgttcga gaacgccggt caggactgct
      841 gcgcgcgcag ccgcatcctc gtcgagcgca gcgtctatga ccggttcatg gagttgctcg
      901 agcccgcggt caagggcgtg gtcgtcggtg cccccggtga cccggccacc gagatgggcc
      961 cgctcgtctc gaaggcccac ttcgacgcgg tcagcgccta cgttccggat gacgcggccg
     1021 tcgccttccg tggcagcaag cccgacggcc ccggctactg gttcgcgccc acggtgctca
     1081 cgccgagttc gcgcaccgac ccggtggtca ccgaggagat cttcggcccg gtcgtcgcgg
     1141 tgctcccgtt cgacgacgag gccgacgcca tcgccctggc caactcgagc atctacgggc
     1201 tcagcggctc gatctggacg cgcgatgtgg gtcgtgccat ccgggtcgcg cgcggggtcg
     1261 agtccggcaa cctctccgtg aactcgcact ccagcgtgcg gtactccacc ccgttcggcg
     1321 ggttcaagca gtcgggactg ggccgcgagc tcggtccgga tgcggcatcc gccttcaccg
     1381 aaaccaagaa cgtcttcatc tcaaccgact aagcggccgg tcgagtagcg cgcagcgcgt
     1441 atcgagaccc ggtttcgata cgcggcttcg ccgctactca accagcagaa aggcacacat
     1501 gaccatcacc aagatcgacc tcacccagcg gctgaagggc aaggtcgccg tcatcacggg
     1561 cggcgcgagc ggcatcggct ttgccacggc gcgacggttc gccgccgagg gcgcgacagt
     1621 cgtgatcggc gacttcaacg ccgagaccgg cgagaaggcg gccgcggagg tcggtgggct
     1681 gttcgtgcag gtcgacgtga ccgacgagga acaggtcaac cacctcttcg acaccgccgc
     1741 ggagaagtac ggctccgtcg atatcgcgtt caacaacgcc ggcatctccc cgcccgacga
     1801 cgactccatc gagaccacgg agctgcccgc ctggaaccgg gtgcaggacg tcaacctcaa
     1861 gagcgtctac ctgtgctccc gcgcggcact gcgccacatg gtcaagcagc agtcgggctc
     1921 catcatcaac acggcctcgt tcgtcgcggt catgggctcg gcgaccagcc agatctccta
     1981 cacggcgtcg aagggtggag tgctcgcgat gacgaaggag cttggcgtgc agttcgcgcg
     2041 gcagggcatc cgtgtcaacg cactgtgccc cggcccggtc aacaccccgc tgctgcagga
     2101 actcttcgcc aaggacccgg agcgggctca gcgtcggctg gtgcacatcc ccaaggggcg
     2161 attcgccgag cccgaggaac tggccgccgc tgtggcgttc ctcgccagcg acgacgccag
     2221 tttcatcacc gcatcgacct tcctcgtcga cgggggtatc agctcggcct acgtgacgcc
     2281 gctgtaatca tggcgacccc gggggaggac gcgacctacg agggcctcgc cgacgaggta
     2341 ctgctcaggc cggtgcgcag cggcaactcg ttcgaggaca ccgtggcacg cctgctccag
     2401 accatccgcc tcggcgtcgt cggcccgggc gagccgcttc cgcccgagcg cgaccttgcc
     2461 gcccgcttct ccgtgagccg cgacactgtg cgcgacgcca tcaagagcct ggcggatgcc
     2521 ggctacctgg tatcccgccg gggcaggtac ggtggcacct tcgtcagcga gccgctgccc
     2581 acgtcgcggg cggacacccc accccccgcc gcgctcgacc tcaacgatgt gctcggcctg
     2641 cgcgagatcc tcgaggtggg ggcggccagg gcggcagcgg cacgcgagct cagcggcgtt
     2701 cagcgcgatc tgctgtggac tcggctgagg gagaccgccg cggcatccgt caccgactac
     2761 cgtcgcctgg actcccggct gcacctgacc atcgctgagg tggtgggcac accgtcgctc
     2821 gtctcgctgc tcgccgacaa ccgcacccgg gtgaacgagc tgctcgacgg catcccgctg
     2881 ctcgcgaaga acatcgagca ctcgaaccag cagcacgagg acatcgtcgt ggcgatcctc
     2941 acgggcaacc ccgaacgagc cgcgctggcg atgggcgagc acctggaggg ctcggcggca
     3001 ctgctgcgcg ggttcttgag ctaactcagc cgcgtgccgc ggcgaggatc ttctcgaggt
     3061 tcttgtcgcg cgtggcatcc gacttcgcgg ccacccacgg ctcgaccacc gcgcgctgct
     3121 tcgagtagga gagagcccgg aacgcctcga gggcgtcagc ggcttcgagt gcgtcagcga
     3181 ccggagcggg cagttcgagc acgcgaggct cggtgtcgtg ctcgagcgtg acggtgatcg
     3241 cgtcgccgcc ggtgagtccc gcctcggcct tgacggctga gctcacgctg atcatgaagg
     3301 cgcccttgta ccagctcacg ctgctgcggt agctgtagtc cccgacggtg acgacaaccg
     3361 ggtagcgctt ggccgggccg agttgggcca tgatctcctc gggcacgacg atgccggagg
     3421 ctgttccccc gcccgtgagg acggtgtcga acgtcacgct catggcgact cagggcgcct
     3481 tgggcgtgcg tggcctcggt gccgccttgg ctggcggcgt ggtcgtggcc ggcgcagcgc
     3541 ttgcggtgtt cggtgcctcg ctcgacaccg cggggctgtt cttcgcgacg tagatctggg
     3601 cggccttggt ggccaccagg aggaaccgca ccagcaggaa gatcagggcg atgaccacca
     3661 acagcatgaa cagccacagc agcccgccga cgatcatcat cgcgaagtgc gcgaaaggtg
     3721 cgaacggtcc cggtccgtca aagttcacgg tccctcctcc agttcgttgg tctgggcatc
     3781 tttgaccacg gtgcccacgg cgatcgccac ggtgagaccc cagctgatcc acatcagaat
     3841 caggcgccag tcgcgcgggc cacgccgcgt cgactgaacg acgccgaaca ctccgaacag
     3901 agcactgagg atgctcgtgt tgaacaagaa cctgcgcacg tgtgcctccc agaccgtcgt
     3961 cgtccacgct acccgcagtt gtcgcgatcg cgcagcccat cagtacagtg gctgatgtgg
     4021 tcaacggatt caccccgcga gcgttcgcat tcttccggga gctggcggcg aacaacaccc
     4081 gcgagttctg gctggcgcac aaggccgagt tcgaggcggt cgttcgcgac cccatggagt
     4141 cgttgctcgc ggcgcttccg gaaccctacg acggattccg ccccttccgc atgaaccgcg
     4201 atgtgcgctt ctccaaggac aagtcgccct acaagacagc gcacgccgcg gtgctcgacg
     4261 accggggggc ggtgctctac ctccaggtgg gcgccgaggg gatgttcgct gccggaggtg
     4321 gctacctgct ggcccccgat cagctggagc gcttccgcgc ggcagtggat gcccccgcgc
     4381 ccggtctcga actcgagcgc atcatcgacg agctcggccg cgatggaatc gacgtcggtc
     4441 ccggcggtgc tgagcccctc aagaccgcgc cccggggcta cgacaaggag catccgcgca
     4501 tcgggctgct gcgccagaag ggcatcgccg cgggcatccg gctcgacccg gaggactgcc
     4561 gcaacggcga cgagctcctg gccacggtgg tcgatttctt cgacgctgtg cagccgctca
     4621 cgtcgtggtt gcgcgaccac gttggaccat ccacggcccc gcgcgggcgc taggggtcgc
     4681 ggcctaggtg agtccgaggt cggcgaggat cgtgtggatg tcgggaggag tcacccggct
     4741 ccagtccatc ccgcggctga agaggtagcg ctcgcgctcc tcgtacagtc gacccatccg
     4801 ctcatcccac tcgtcattga aattgaagta ccaccccggc agcggggtca gttccccgac
     4861 gactgggccc cgatcggtcg ggtagaggtc gacgcgaagg tacggcagcg gtagggcggc
     4921 accgatggcg cgggcgatgc cgaggagttg ctcggcattc tcgggtgcgt tcagtgttgg
     4981 atcgagcttc tcggtgtgga tgccgacgtt gaccttctcc cagacgtcat cgaaccactg
     5041 aaatccccgg ggtctggtgc tcctcacgaa cgagcaggcc atgtagtcgc cgaacatcga
     5101 gatcttcacg tcgtcgacgg gccggtctga cccgtctgcg gctcgcaaca gctcttcgcc
     5161 gatccaacgg tcggcgaatc cagccttctc ccgttcggcg tccaacctcg cggtgagttc
     5221 cgcgaaggtc aactcggtgg ccgtgatctg ctcgaaccag cggtggccgc cgagcgggac
     5281 cagcgcaaca acgcctcgat tgttgtacga cctcgccggc ttgagcactg tcggttcgac
     5341 aaggtgatcg ggggtgaggc gggagagttc accgatcact cggaagtcgc gggggagtgg
     5401 ggcaccgagc gcctcgagaa gccgatgact gcgttccttg tcgaacatcg cggagatgac
     5461 ggggtagtcc ttgcggcggt tggccgtggc gatgcacgcg ctgaacgaac tcaccgtgga
     5521 tctcctcatg gaagatgacc ataacgtgtg aggacgttac tgagagttca cccacaactg
     5581 accgatcgga cagtttcctg accgctcggt cagttatcgt ggaagcatgt caagccaaga
     5641 ggacctccgc accattgcgc tcgcggaatt cgcgaccgcc gggtacgcgg gcacctcgct
     5701 gcagcgcatc gctgagcttg ccggactctc caagtcgagc gtgctctacc actacgcctc
     5761 caaggaggcg ctcctcgagg cggccatcgg cccggcgatc gaccgcatga tcgcgatact
     5821 cgagcccctc gtcggactcg agctcgatga cgacgcccgc acgaccttcc tcgaggcctt
     5881 cgtcgacttc ctgctcgacc accgactcga agtgcacatg ttcatcaacc agggtccgtc
     5941 gctcgtcgac gtgcccgtga tcgaccgcgc caacgcgctc gtcttccgcc ttgccgagtt
     6001 cttctcggcc aactccgcga gcctcgagga gaagatgcgc ttcggcatcg ccctcggcgg
     6061 cgccgcctac atgctgtgca ccgtgcactc gctcgacctt ccggtcgagc cccgtgaaga
     6121 gaccagggtt gctctcctca cgatcatggg ggagctcctc gcccccgttc gtatccgcta
     6181 gatctcgact gtctcgactg tctcgacaag ctcgacaacc gacaaattcg atcaccaaca
     6241 ccaccctggg gtaaccgtgg caacacttct ctaccgactc ggccgcttct cgtaccgtca
     6301 cgcctggcgc gtgatcgggg tgtgggtgct cgtgctcata gcggtgctcg gcgcgggcat
     6361 cggtctcggc ggcaagacgc aggagtcgtt cgccattccc ggcaccgagt cgcagcaggc
     6421 gctggaccga ctcgaggccg tgttcccctc ggtcgccggc gcgtcggcga ccgccgtcgt
     6481 ggtggctcct gccggggcat ccatcggctc ggagaaggcg accatcgaag agctcgtgac
     6541 cgccatcgag aaggtgcccg gcatcgactc ggcgatgagc ccgttcgacc agtacgcggg
     6601 caaggccatc tccgacgacg gcacgatggc catcatccgg gtgcagttcg acggtccgtc
     6661 gaccgacgtc acggatgcca cgatcgccga cctcaaggcg acggccgaag tcggcaccgc
     6721 tgagggcctc caagtcgagt tcggcggtca ggtcttccag gagaccagcg tcgccatcac
     6781 catcaccgag gtcttcggtg tcatcttcgc cggcgtggtg ctcgtgatca ccttcggatc
     6841 actgctcgcc gcgggcatgc ccctgctctc cgcgctgctg ggcgtgggca tcgtcatggg
     6901 tgcgatcatg accctctccg cgttcacgac cgtatccagc tcggcaccgc tgctcgccct
     6961 catgatcggt ctggcggtcg gcatcgacta tgccctgttc atcctctcgc ggcatcgaac
     7021 tcagctcgcc aagggcgagg accccgagga atccgcggcg atggccgtgg gcactgcggg
     7081 aagcgccgtc gtcttcgccg gcgtcacggt catcatcgcg ctgctcggac tgctcgtggt
     7141 gggcatcccc ttcctctccg tgatgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnncgg cgaggtctcg acgagctcga ccaccgagcg cccgcaccgc accatggggc
     7321 ttcgctgggt caccggcgtc atgaagcggc ccgtactggc caccctcggc gtgatcacga
     7381 tcctcggaac tctcgccatc ccggcgatga gcctcgacct caacctgccc gacggtggtt
     7441 cacagcccgt cgactccacc cagcgcaagg cctacgacct cgtgagcgaa ggcttcgggc
     7501 ccggtttcaa cggtccgctg atcgtcgcgg tcgacatcac ccagacggtc acgatcatgg
     7561 aggacctcga ttcgatcgga tcccagctgc gtggcctggc cgatgtcgcc tacgtgtcgc
     7621 agggcttccc cgacgagggt ctcgacacgg cgatcatcca ggtcaccccg tcatcggccc
     7681 ctgactcgct cgagaccaag cagctcgtgc agaccatccg cgacctcgcg ccctcgatcg
     7741 cggcgaagta cgacaccccg atcaccgtga ccggcacgac ggccatcggc atcgacatct
     7801 ccaaccgcct caccagcgcg ctggtgccat tcggtctcat cgtcgtgggg ctctcgatcg
     7861 tgctcctcat gatggtgttc cgatctgttc tcgtgccgat caaggcggcg ctcgggttcc
     7921 tgctgtccgt cgtggcatcg ttcggtgtcg tcgtcgccgt cttccagtgg ggatggctcg
     7981 gcgacatcat cggggtcgag aaccccggac cgattctcag cttcatgccg atcatcctca
     8041 tggcagtgct cttcggtctc gccatggact acgaggtgtt cctcgtctcc ggtatgcgcg
     8101 aggagttcgt caagaccggc gacgctcgct acgcggtcaa gcacggcttc gccaacggcg
     8161 cccgtgtcgt cacggcggcc gcgctcatca tgttcttcgt cttcttcgcg ttcgttcccg
     8221 agggcagcgg catgatcaag cccatcgccc tgggactcgc cgtcggtatc gccttcgacg
     8281 ccttcctcgt gcgcatgacc ctggtgcccg cgctcatgac cctgttcggc aaggcggcct
     8341 ggtggatgcc ccgctggctc ggtcgtctgc tgccgaacgt cgacatcgag ggtgaacagt
     8401 tgcgcgagca caagcacgcc gtcgactggg cacgcggcga gggtgagctg gcgctcagcg
     8461 ccgagtatct cgtcgcagga tcccgagagc acgaggtggg accgctgtcg atcagtgttc
     8521 cgcgcggtgc gatagtcgtc gccagcggtg acccgctcga ccgtcgactc gtggccgcca
     8581 ctctctcggg acgcctcgac ccggtgtcgg gccgggcaca ggtggccggg catccgctgc
     8641 cgtctgaggc cgccgacgtg cgctcgctcg tcgcgctcgc cgacatcggc gggtcgcagc
     8701 gctcggagac ctccgtcacg gtcggagaac tgctcgtcga gcggctcgag atgacgctgc
     8761 cgtggtaccg cctgtaccgc acgcgccgtg ctgcgcgccg ctggctcgcg cgcatcaacg
     8821 atgtgctcgg gtccgccgca tcgacggtgc gcgccgagag cacgctcgtc gagctgcccc
     8881 agctcgaacg cgccgtcgcc ctcgccgccg tcgccctggc cgagcgcacc cccgtggtga
     8941 tgctcgacca gctcgatccc ttcgccaacc ccgaggacga ggcggccttc atcgccgcga
     9001 tcaacgccct cgctcccgcc acgaccactc tggtcgtcgg cacgccgttg cccggtcggg
     9061 cgactgatgt cgcgagcaat cgcccgcgca tcgacatcga tctgtactcg ctcagtgcct
     9121 cggaaggatt cgcacagtga ccaccagttg gctccgacgg gtaggaatcg ccgccgtcgt
     9181 cctcatcccc ctcgcctttg cgggtctctt cgtgggtgcc gtcgggcagg gcaagtccgc
     9241 catcgactcg attccggtgg cgatcgtcaa ccaggacacc ctccagaagg tgaccaacgc
     9301 cgacggatcg gtgaccaacg tcttcgctgg gcgtcagctc gtcaccgagc tgaccggcag
     9361 cgagggcttc gactggacca tcaccaacgc ggccgacgct gaggcgaagc tcgcctcggg
     9421 cgaggtgtac gccgtgctca ccgttccgtc gaacttctcg gactcgatcc tctcgttgtc
     9481 gagcgccgac ccgaagaagg ccgacatctc gatccgcacg gatgacacgc acagctacct
     9541 caccggctcg gtcgcacagg tcgtcggcca gaccatgacc gacaccttcg gcaaggccat
     9601 caccgcccag tacatcggcg gcatctactc gagcctcggc gatctcggca cgtcgctcac
     9661 cgcggccgcc gacggtgcga cgtcgctcgc caacggggcg acctccttgt cttcggggct
     9721 gacccagtac acggacggcg tcggttcgct tgcctccggt ctcagccagc tcgaggatgg
     9781 tgcggctcag ctgagctcgg gtgccgcgac ctacaccggc acggtgtccc agatctccgc
     9841 cggactcact gccctcgcgc ccgcaatgtc gacggccgat cccgctctgc aggcacagtt
     9901 caacgcgctc gtcgccggtc tctcgcagac ggcggccggc ggctcgtcgc tcaccacggg
     9961 agtcgccggg gtccagtccg gcatctcgca gagcgcgagc ggagcgtggc agcttgcgaa
    10021 tagctcggac cagctcacca gcggtgcgag ccagctcgcc tccggggcca ccgacctggc
    10081 gaccggcctg cagtcgggcg cagaccagct gccctcgtcg aacgccgaca cagcggccgc
    10141 ctcggccgag gtcgccgccg atccggtcgg gctgacggtc tcaaccgaca acgcggtgtc
    10201 tcaggtgggc cagatcgtcg cgaccttctt tgtgccgctc ggcctgtggg tgggagcact
    10261 cgcggtgttc cttgtcatgc gtccggtcac tcggcgcgcg ctcacctcga cggcccgcaa
    10321 cggtcggctc gtggcatccg ccatcggcaa ggccagcttg atcaccgggg cccaggccgt
    10381 gcttctggtc gccttgctgc acggtgcgct gggcgtcgcg tggagcctgc tgcctgccac
    10441 tctcggcttc tcgctgctga tcgcgatcgc gttcaccgcg ttccactacc tgctgaccat
    10501 cgggctcggc cgggcagggc tgatcgtgtc gctgttcctg ctggcggtgc agatcacgtc
    10561 caccggcggc atctacccga tcgagctgct gtcgacgccg ttccaggtcg tgagcccgtt
    10621 ccttccgctc acctacgcgg tgcagggaat gcagggcatt ctcgccgggg gcaacccggg
    10681 cggcgtgatc gcagcggcgg ccgttctggc ggccttcggg atcggctcga cagtgctcgc
    10741 cctgttcgcc atccggcgca cgcggcgagc gggggcgatc ggactgctgc ccgcggtcgc
    10801 ctcgtagctt cccgggacgc gcgccttcgc ggggtctggc ctcaggtcac gcgggcaact
    10861 cggcgcgccc gaggttgcga cgaatcagat atcttcagca ccctcccgtt aagcaggatc
    10921 accgagaaag actcgcggat cctgcataac gggaccgtct gcctcttgat gcgagaagat
    10981 c
//
LOCUS       DAOSOU010000051        10924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_5989_length_10924_cov_6.335633, whole genome shotgun sequence.
ACCESSION   DAOSOU010000051 DAOSOU010000000
VERSION     DAOSOU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5989_length_10924_cov_6.335633"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLW15_04405"
     CDS             complement(<1..699)
                     /locus_tag="PLW15_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33675.1"
                     /translation="MSAIISTRRLVGDHLRSGWGASLLVAVLVMLTVFAVAVAPRAFS
                     AVATAELRHQLAAAPEEQLNLRGDGQIGLPGLGEGATAEALLGPTDEAITRIPSLLPR
                     PLRDGVGEPTWLIRTVGIAGSNPDFPYAFLAIRLAIDLTWMTHITFVSGQQPAPWQAD
                     SGAPIEIALSATTAAAIGAETGDILDTGVGPCLVSGIYEPTQPDDSYWRHAFDLGSPI
                     EKRQSGQRPTIQASA"
     gene            complement(696..3761)
                     /locus_tag="PLW15_04410"
     CDS             complement(696..3761)
                     /locus_tag="PLW15_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33676.1"
                     /translation="MSLSRLRGSAPILATIVAVVALVSAFATATVIVLDERTTGGVTT
                     ELVQRSGSFVALRATFDRASDAEQQDAAVRAAVDRTFAGTGVHFTVIRALESKAVYST
                     VSSDAVETSGSATVSTFSDLASHAVFETGSVAHDEFEVAVPADDAELLGLRVGDELII
                     SDLPFTVSGTWRAVDSLDPRWYGDARVASGGGDPLGPFVIDESAWARIGSVPLAIWTI
                     VPESIDSITATNLTAVTSAWAGVMQSWRGTVPEYESMDVEQRLSRTLDELAGRIAGLR
                     AIEPVAFTLMVGSALVGLSQIVQLLVVTRERESALFWARGQSPRAVAARITAEVGISA
                     VVGAVVGVGAVAGVAVALNQAGTLSGLLPLGVVVPTLVIVGATAIAAVFAARSASAIA
                     NPSRGGRTTGRTGRVAVPGVVVLVTIGAALAVWQLRLYGSPIVGAADGGRIIDPVAVA
                     APAAALVAIVLISLAAFPSIVRLIARRAARGGLRPFLAARTLARNTRRVVAPLVVVAL
                     AVGSATIAATFSETWSRSFAQASQLYAGADVHVSSPLVPISPTQVEAVARTDGVTAVA
                     PLDVQILSVGTLRGSTLAATPEAVRELATTASGTFSPADAAAAIQMDPVGPQVPEGTT
                     QLTLRVEALGFVAPPTLVAWIDDPLGRLHSVSFGDPTSEAEGVLVYTADPGRIAGTIA
                     SIDVGMPRQSVEDRPSFRLLELTAASRSTQESLALDQFWVLDTLSIHFDAPKPLTDAP
                     GFVLDRQLTLLRMTASLDGGATDEVRPRLLVSAALASGLNLEIGDTVGFTIRKGQERL
                     VGIVAGVVDSIPGARSSNAVLMDLSVNNHFQLRSDLVPGTTTDLWVAAEHPDRVRADI
                     GASLPADTRIDTRTDPFGAQVLGAASVALWAAALCCVLIAIVAVASASRSRLRWGREE
                     IGSLRAIGVGARDQSSIILRELGVVLLVATVTGVIAGAVVSVATVPQLVRAAVDRAYL
                     SGEIALAIEWTGFIALVGVLAAGTGVVLIGLSRRVRRLASTALPGQGDQ"
     gene            complement(3842..4930)
                     /locus_tag="PLW15_04415"
     CDS             complement(3842..4930)
                     /locus_tag="PLW15_04415"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HOT33677.1"
                     /translation="MKHVVLALGLGIAGVLVFVQPAWAAASVAPPIPVGVAPNEVTFS
                     PDGTVAYSANSGSNDISVIDVASGTTVGLPIPVDAQPVSIAFVPNGSAAYVASNGGTV
                     SVIDTASALVTDTIAIAGASFIEITPDGATAYVASFGTNTVVPLDVATGVAGTPIPVG
                     SGPTGLAISPDGSRLYVANTWDGTVSAIDIATNTVITQIAVGPRPFQLTVSPDNSEVW
                     VAVGDINALAVIDAATLETVATIAVGQNPRSVTISPDGTTALAESYFDNAVTVVDVAT
                     RVVTSVISIGLNPVYVQFSPDGSRAYVAVAGDWTIGVILFDPALYPQPAAPALARTGE
                     NLGGLVPIAGGLLALGIVLRNRRKHSTP"
     gene            5016..>5814
                     /locus_tag="PLW15_04420"
     CDS             5016..>5814
                     /locus_tag="PLW15_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT33678.1"
                     /translation="MLEPLADLDRHATERPDSPALCSPRRQFSFAQLRTAVLAVAARL
                     DRAGVASGTLVGIDLPTALEWIVDLALLRLGARSVSLRGVTNPGPTELDALITDPGRR
                     AAPAALTIEADDLWLDSAIARGDDPPPVADFGGDDAIFRLILTSGTTGGVPKAAAYSL
                     GALEYRSAEQHVHWTDGRPELTLIGLSTTGGFHAAIASLRLGVPYLAVDRIDAETMRF
                     AAEQKIEVLCGSPSQVARAIHAARDADIELPSLTEVRLAGAGPTAGLL"
     assembly_gap    5815..5914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5915..6257
                     /locus_tag="PLW15_04425"
     CDS             <5915..6257
                     /locus_tag="PLW15_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33679.1"
                     /translation="TDGELVLGGRDTEIFNLGGVKIDPATVDEPALAFPGVIDAAAFA
                     VERVPGLPEVGLAVVTQVGCDLRELDRQLRARLPLGHPTAFWSVAEIPRTRLGKPMRA
                     MLSEQFARLPR"
     gene            complement(6254..7603)
                     /locus_tag="PLW15_04430"
     CDS             complement(6254..7603)
                     /locus_tag="PLW15_04430"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOT33680.1"
                     /translation="MPEAGTSFTRSRAVRVASGLASFGVVVGFSLVAASPASAATDAD
                     CTPLNTVDATTGTSTDIQTLLTASTPVICLSGTFTLTAGLTYDYDVTLHGLPSATLDG
                     DGSYGILTDTGTHTLIVENLRFTNGNAFDGGAINGYGVLVNNSSFDNNSATSFGGAIA
                     AYGTEINNSVFEDNTAAFGGAVAAGFVGVSASTFTQNSADASGGAIYGYGGGIGAVAV
                     DSSTFEANTAQFVGGAIASYGSLAVDNSTFVGNSTEDEFGQGGAIGAESGTVFQSTFL
                     DNSSGSGSAASIYKSSDTELTLRGNIFAGSVDEHLFADGTGQFADAGGNLFTTSEATE
                     SSLSGVQPSTLFDLTTLAIFNGATLADNGGPTFTVALYAGSPAINAVPADADSLPVDQ
                     RGVARPDVSDAGAYEFVAPVLAATGSVPSGILGGAAALLLGAGALAVGLARRAVRSR"
     gene            7618..9084
                     /locus_tag="PLW15_04435"
     CDS             7618..9084
                     /locus_tag="PLW15_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT33681.1"
                     /translation="MTTSHFRKDFMLAPLADLERTAAQMPEAPALVSPARRYTFAELK
                     RVVDAAAIRLRAEGVTPRSTVAVDLPSLNEWIIDLALMRLAARSVSLRGVPESSGLRA
                     DVLITESGRRATPAAREAVVDERWLVEAVSGASGTAPLVEYPRPDSIVRLMMTSGTTG
                     TPRAAAYSAAALEYRTEGLHRYWSDGRAEVNFMALSTTGGFHTAIANLRHGQAHRAVD
                     RIDADTMRFVAAEGVQVLCGSPQQMAHAVGVLDEARITLPQLEEVRMAGATPGDALLR
                     RIAEVLAVPVRSVYGSTEGGGVTMRMLAAGDDLANVGPALQGSELEVVDENGAPLPLG
                     VEGTVRYRSPGQTSGYLVGDTVEPFPGGWFAPGDLGMLEPDGSLVLAGRSAEILNIAG
                     QKVDPAVVDGLAAQFPGVRDAAAFGFERPTGLAELGLAVVADAECDLRALDRDLRARL
                     PGAHPTTFWRVSEIPRNRMGKAERGVLAEAFGRTGSVR"
     gene            complement(9081..9497)
                     /locus_tag="PLW15_04440"
     CDS             complement(9081..9497)
                     /locus_tag="PLW15_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOT33682.1"
                     /translation="MSPTESFSAEERAAMKERAKELKAQAKAQDLLKDLLAKIAEMPE
                     HDRVIAEAIHRIVTTTAPELTPKTWYGQPAYAKGDQIVCFFQSAAKFGVRYATLGFNE
                     AATLDDGSMWPVAFAIKDLTPADETLIAQLVTKAVG"
     gene            9629..10102
                     /locus_tag="PLW15_04445"
     CDS             9629..10102
                     /locus_tag="PLW15_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT33683.1"
                     /translation="MTDNPTSTVDETTFSVRRTITIAAPIESVWAAVTEAQHISKWFG
                     EAALNGSGVGALGTLSWDDYGTIPIRVEAMDAPTLVSYRWGNDDASGVLADQIDDDHS
                     TVFTFTLEPIETGTRLTVVETGFDTTSDPIANLESHREGWDSELDELVALLEAGL"
     gene            10099..10413
                     /locus_tag="PLW15_04450"
     CDS             10099..10413
                     /locus_tag="PLW15_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT33684.1"
                     /translation="MSEVLVPVFAALGDDTRWSILAALGQGDASASGLASRLPVSRQA
                     IAKHLTILEEVGLVEPLRVGREVRYRVVGAQLEATARRLDELGFAWDRRLAAIKEIAE
                     RL"
     gene            10413..>10924
                     /locus_tag="PLW15_04455"
     CDS             10413..>10924
                     /locus_tag="PLW15_04455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT33685.1"
                     /translation="MTTSHVIKPLTPETFPAWLALAQKHNGVWGGCYCSYFHSDTAQT
                     VKSEYDRATFKERLVAEGVAHAALVFDGDTAIAWCEYGSPLELPGIYHRKQYDAGETK
                     PAPWRITCFFVDRDHRRSGVAREALDGALELIAQAGGGEVVSFPNELAPGKRTSSSFL
                     HNGTRAMFEKA"
BASE COUNT         1738 a         3691 c         3783 g         1612 t
ORIGIN      
        1 ggccgacgcc tgaatcgtgg gccgttgccc ggattggcgt ttctcgatcg gcgagccgag
       61 gtcgaaggcg tggcgccagt agctgtcgtc gggctgggtg ggttcgtaga tgccggagac
      121 gaggcacggc ccgaccccgg tgtcgaggat gtcgcccgtc tcggccccga tcgctgccgc
      181 ggtggtcgcc gacagggcga tctcgatggg cgcaccgctg tcggcctgcc acggtgcggg
      241 ctgctggccg gagacgaacg tgatgtgcgt catccaggtc aggtctattg cgaggcggat
      301 ggcgaggaac gcgtacggga agtcggggtt cgaccccgcg attcccaccg tgcggatgag
      361 ccaggtcggc tcgccgacac cgtcacgcag cgggcgcggc agcagcgacg ggatgcgggt
      421 gatcgcttcg tcggtgggac cgagtagcgc ctcagccgtc gcgccctcgc cgaggccggg
      481 aagcccgatc tgtccgtcgc cccgcaggtt cagctgctcc tcgggggccg cggcgagttg
      541 gtgccgcagt tcagcggtcg ccaccgccga gaatgcgcgc ggcgcgaccg caaccgcgaa
      601 tacggtgagc atcacgagca ccgctaccag cagggatgct ccccagcccg aacgaagatg
      661 gtcgccgacg agacgcctcg tgctgatgat ggcgctcatt gatcgccctg ccctggcaga
      721 gcagtcgacg ccagtctgcg cacccgccga ctgaggccga tcaggaccac accggtgccc
      781 gcggccagca cgccgaccag agcgatgaaa ccggtccact cgatcgcgag ggcgatctct
      841 ccggagagat aggcgcggtc gacggccgcc cgaaccagct gcggaaccgt cgccaccgac
      901 acgaccgcgc cagcgatgac gccggtcacc gtggccacca ggagaacaac acccagctcc
      961 cgcaggatga tcgatgactg gtcgcgcgca cccacgccga tcgccctcag cgagccgatc
     1021 tcctcccgac cccagcgcag ccgagagcgc gatgccgagg cgacggccac gatggcaatc
     1081 agcacacagc acagcgcggc ggcccacagc gcgaccgacg cggcacccag cacctgagcg
     1141 ccgaacgggt cagtgcgagt gtcgatgcgg gtgtcggcgg gcagcgaggc tccgatgtcg
     1201 gcacgcaccc ggtcgggatg ctccgccgcc acccacaggt cggtcgtcgt tcccggcacc
     1261 agatcggatc ggagctggaa gtggttgttc accgacagat ccatcagcac ggcattgctc
     1321 gagcgagcgc ccgggatcga gtcaacgacc cccgcgacga tgcccacgag tcgttcctgt
     1381 cccttgcgga tggtgaagcc cacggtgtcg ccgatctcga gattcaggcc gctcgcgagg
     1441 gccgccgata ccagcaggcg cggacgcacc tcgtccgtcg ccccgccatc aagcgaggcc
     1501 gtcatgcgca gcagggtcag ctggcgatcc agcacgaaac cgggtgcgtc agtgagtggc
     1561 ttcggcgcat cgaagtggat gctgagggta tcgagcaccc agaactgatc gagggccaga
     1621 ctctcctggg tggaccggct tgcggcggtc agctcgagca gccggaaact cgggcggtcc
     1681 tctaccgact gccgcggcat cccgacgtcg atcgaggcga tggtgcccgc gatcctgccc
     1741 ggatcagccg tgtagaccaa gaccccttcg gcctcggatg tcggatcgcc gaacgacacg
     1801 gagtgcaggc gtccgagcgg gtcgtcgatc cacgccacga gggtgggcgg tgcgacgaat
     1861 ccgagtgcct cgacccgcag ggtgagctgc gtcgtgccct ccggcacctg ggggccgacc
     1921 gggtccatct ggatggccgc cgcggcatcc gcgggactga aggttccgct ggccgtcgtg
     1981 gcgagctcgc gcaccgcctc cggcgtggcg gcaagcgtgc tccccctgag cgtaccgacc
     2041 gacaggatct gcacgtcgag tggggcgaca gcggtgacgc cgtcggtgcg tgcgaccgcc
     2101 tccacctgag tgggcgagat gggcacgagg ggcgaggaga cgtgcacatc cgcgccggcg
     2161 tagagctgcg acgcctgggc gaacgacctc gaccaagtct ccgagaacgt cgcggcgatg
     2221 gttgccgatc ccaccgcgag cgccacgacg accagcgggg caacgacgcg acgcgtgttg
     2281 cgggcgaggg tgcgtgctgc gaggaacggg cgcagcccgc cgcgcgcggc acggcgggca
     2341 atcagccgca cgatcgacgg gaacgccgca agcgagatca gcacgatggc gaccagtgcg
     2401 gccgctggcg cggcgacggc aaccggatcg atgatccgac ccccgtcggc ggcgccaacg
     2461 atcggggatc cgtagaggcg cagttgccac accgcgagcg cggccccgat cgtgacgagc
     2521 acgacgacgc ccggaacggc gacacggccg gtgcgacctg tcgtgcgtcc gccgcgtgac
     2581 gggttcgcga tggccgacgc cgatcgggct gcgaacactg ccgcgattgc ggtggctccc
     2641 acgatgacca gcgtcggcac gacgacgccg agcggcagca gccctgacag tgtgcccgcc
     2701 tggttgagcg cgaccgcgac ccccgcgacc gcaccgacgc ccaccactgc cccgaccacg
     2761 gcgctgatgc cgacctccgc ggtgatgcgg gcggccacgg cccgtggcga ctgaccgcgc
     2821 gcccagaaca gggcggactc gcgctcccgg gtgacaacca gcagttgcac gatctgcgag
     2881 aggcccacca gcgcgctgcc caccatcaac gtgaacgcga caggctcgat tgctcgcaga
     2941 cccgcgatgc gaccggcgag ctcgtcgagc gtgcgactca gtcgctgctc cacgtccatg
     3001 gactcgtact cgggcacggt gccgcgccac gactgcatga ctccggccca cgcggatgtc
     3061 accgcggtga ggttggtggc ggtgatgctg tcgatcgact ccggcacgat cgtccagatc
     3121 gcaagcggaa cggagccgat gcgggcccac gctgactcat cgatcacgaa ggggccgagt
     3181 ggatcaccgc cgccggaggc gacccgggcg tcgccgtacc agcgcgggtc gagcgagtcc
     3241 actgcccgcc aggttcccga taccgtgaat ggcaggtcgc tgatgatcag ttcgtcaccc
     3301 acccgcaggc cgagcagttc ggcatcgtcg gccggaactg ctacctcgaa ctcatcgtgg
     3361 gcgaccgacc ccgtttcgaa caccgcgtgg ctcgcgaggt cggagaaggt ggacaccgtc
     3421 gccgaacccg aggtctcgac cgcgtcactc gagacggtgc tgtagacggc cttcgactcg
     3481 agcgctcgga tgacggtgaa gtgcacgccc gttccggcga acgtgcggtc gacggccgct
     3541 cgcaccgcgg catcctgctg ctcggcatcg ctggcacggt cgaaggtcgc acgcagagcg
     3601 acgaaggagc ccgagcgctg caccaactcg gtggtcacgc ccccagtcgt gcgttcgtcg
     3661 aggacgatca cggtcgccgt ggcgaaggcc gagacgagag cgaccacggc gacgatggtc
     3721 gccaggatgg gagcgctccc gcgaagtctc gacagtgaca cgggacttcc cccttccaga
     3781 gcgcccgcac cggtgtggga ctgtgaggaa gtctacggag cgcgctactc ggccccaact
     3841 ctcatggagt cgaatgcttc ctgcggttgc gcagcacaat cccgagggca agcaatccac
     3901 ccgcgatcgg caccagccct cccagattct ctcccgttct ggccagcgcg ggcgctgcgg
     3961 gctgcgggta cagcgcgggg tcgaacagaa tgacaccgat cgtccagtcg ccggcgacgg
     4021 cgacgtaggc gcgcgagccg tcggggctga actgcacata gaccgggttg aggccgatcg
     4081 agatcaccga cgtgaccacg cgggttgcga catccaccac cgtcacggcg ttgtcgaagt
     4141 acgactcggc gagggcggtc gtgccgtcgg ggctgatggt caccgagcgc ggattctgac
     4201 cgaccgcaat cgtcgccacc gtctccagcg tggccgcatc gatcaccgca agggcgttga
     4261 tgtcgccgac ggccacccag acctccgagt tgtccgggct cacggtgagt tggaatggcc
     4321 ggggacccac ggcgatctgc gtgatcaccg tgttcgtggc gatgtcgatg gcggagacgg
     4381 tgccgtccca cgtgttggcg acgtagaggc gagacccgtc ggggctgatt gccaggccgg
     4441 tgggaccgga gcccaccggg atcggcgtgc ccgcgacacc ggtcgcgacg tcgagtggca
     4501 cgaccgtgtt ggtcccgaag ctcgctacgt acgctgtggc gccgtcgggc gtgatctcga
     4561 tgaaggatgc gccggcgatc gcgatcgtgt ccgtcaccaa cgcgctagcg gtgtcgatga
     4621 cactgacggt gccgccattg ctcgccacat aggcggcgct gccgttgggc acaaaggcga
     4681 tcgacacggg ctgcgcatca acgggtatcg gaaggccaac ggtagtgccg ctggcgacgt
     4741 cgatcaccga gatgtcgttg ctgccgctgt tcgcggaata ggcaactgtt ccatccgggc
     4801 tgaacgtcac ctcgttgggt gcgacgccca cggggatcgg cggtgcgacg gatgccgcgg
     4861 cccacgctgg ctgcacgaag acgagaacgc cggcgatacc gagacccagc gcgagaacca
     4921 cgtgcttcac attgcctccg tcacggggtt cacccccgcg gcgagactag cagttgggcc
     4981 cgccgcacga gggttgcggc ggatactctg aatccgtgct cgaacccctc gccgacctcg
     5041 accgccacgc gaccgagcgt cccgactctc ccgcgctgtg cagtccgcgc cgacagttct
     5101 ccttcgcgca gttgcgaacc gcagtgctgg cggtcgcggc gcgcctcgac cgggcgggtg
     5161 tggcatccgg aaccctcgtc ggtatcgacc tgccgaccgc gctcgaatgg atcgtcgacc
     5221 tcgccctgct gcgcctcggc gcgcgctcgg tgtcgctgcg tggcgtcacc aacccgggac
     5281 ccaccgagct ggatgcgctg atcaccgacc cgggacgccg cgccgcgccc gccgccctca
     5341 cgatcgaggc cgatgacctg tggctcgact ccgccatcgc ccgcggtgac gacccacccc
     5401 cggtcgcgga cttcggcggg gacgacgcga tcttccggct catcctcacg agcggcacga
     5461 cgggtggcgt gccgaaggcc gccgcctact cgctcggcgc gctggagtac cgcagcgccg
     5521 aacagcacgt gcactggacg gacggacgcc ccgaactcac cctcatcggg ctgtcgacga
     5581 cgggcggatt ccacgccgcc atcgcgtctc tgcgactggg agttccctac ctcgccgtgg
     5641 accggatcga tgccgagacc atgcgcttcg ccgccgagca gaagatcgag gtgctctgcg
     5701 ggtcgcccag ccaggtggcg cgagccatcc acgcggcgcg cgatgcggac atcgagctgc
     5761 cctccctcac cgaggttcgg ctggccgggg ccgggccgac tgcagggctg ctcgnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaccga cggagagctc gtgctcggcg
     5941 gccgggacac cgagatcttc aacctcggcg gggtcaagat cgaccccgcg acagtcgacg
     6001 agcccgcgct cgcgttcccc ggcgtgatcg atgcggccgc gttcgcggtt gagcgcgtcc
     6061 cgggtctacc ggaggtcggc ctcgccgtgg tgacccaggt ggggtgcgac ctgagggagc
     6121 tcgaccgtca gcttcgcgcg cggctgccgc tggggcatcc gaccgccttc tggagcgtgg
     6181 cggagattcc gcgcacgcgc ctgggcaagc ccatgcgggc gatgctcagc gagcagttcg
     6241 ctcggctgcc ccgctagcgg ctgcggaccg cgcggcgcgc caggccgacg gcgagcgctc
     6301 cggcgccgag cagcagggcc gctgcgccgc cgaggatacc ggacggaaca gagccggtcg
     6361 cggcgagcac aggggcgacg aactcgtagg ctccggcgtc actgacgtcg gggcgtgcaa
     6421 ccccacgctg gtcgaccggc agcgaatccg catcggcggg aacagcgttg atggccgggc
     6481 tgccggcgta cagcgcaaca gtgaaggtgg gtccgccgtt gtcggcgagg gttgcaccat
     6541 tgaagatggc caacgtcgtg aggtcgaaca gggtgctcgg ctgcacaccc gaaagcgatg
     6601 actcggtcgc ctcggatgtg gtgaacaggt tgccgccagc gtcggcgaac tggcccgttc
     6661 cgtcggcgaa gaggtgctcg tcgacggacc ccgcaaagat gttgccgcgc agtgtgagtt
     6721 cagtgtccga cgacttgtaa atcgacgcgg cgctaccgga tccgctgctg ttgtcgagga
     6781 aagtcgattg gaagacggtg ccgctctcgg ccccaatggc gccgccctga ccgaactcgt
     6841 cttcggttga gttgcccacg aaggtggagt tgtccacggc gaggcttccg taggaggcta
     6901 ttgcgccgcc aacgaactgc gccgtgttgg cctcaaacgt cgacgagtcg acagccactg
     6961 caccgatgcc gccaccgtag ccgtagatcg caccaccact ggcatcggca ctgttctgag
     7021 tgaacgttga cgcggacacg cccacgaatc cggccgcgac cgcgccgccg aaagccgcgg
     7081 tgttgtcctc gaacaccgag ttgttgatct ccgtgccgta ggcggcgatg gcaccgccga
     7141 atgaggtggc actgttgttg tcgaaactgg agttgttgac gagcacgccg tagccattga
     7201 tggcaccgcc atcgaaggcg ttgccattgg tgaagcgcag gttctccacg atcagggtgt
     7261 gggtgccggt gtcggtgagg attccgtaag acccgtctcc atccagagtc gcgctcggca
     7321 gtccgtggag ggtcacgtcg tagtcatacg tgaggccggc cgtgagcgtg aacgttccgc
     7381 tgaggcagat caccggggtg cttgcggtga gcagggtctg gatgtcagtc gaggtgccgg
     7441 tcgtcgcatc cacggtgttg agcggcgtgc agtcggcgtc ggttgccgcg gacgccgggc
     7501 tggctgcgac gagcgagaac ccgacgacga cgccgaagga ggcgaggcct gacgcgacgc
     7561 gcacggcgcg cgagcgcgtg aatgaggtac ctgcttcggg catggcgaca gtctagtgtg
     7621 acaacgagtc acttcaggaa ggacttcatg ctcgccccgc tggccgatct cgaacgcacc
     7681 gctgcccaga tgcctgaagc gcctgccctg gtctcgcccg cccggcgtta caccttcgca
     7741 gaactgaaac gagtcgtgga tgccgcggcg atccgcctcc gcgccgaggg cgtgacgccg
     7801 cgcagcaccg tcgccgtcga cctcccctcc ctgaacgagt ggatcatcga cctcgcgctc
     7861 atgaggctgg ccgcccggtc ggtgtcactg cgcggggtcc ccgagtcgag tgggctgcgc
     7921 gccgatgtcc tgatcacgga gtcgggccgg cgggctacgc cggcggctcg cgaggcggtg
     7981 gtcgacgaac ggtggctggt cgaggcggtg agcggggcat ccggtacggc ccctctggtg
     8041 gaatacccgc gacccgactc gattgtgcgg ctgatgatga ccagtggaac gaccgggact
     8101 ccacgagccg cggcctactc ggccgccgcg ctcgagtacc gcaccgaggg actgcaccgg
     8161 tactggtccg acggccgggc cgaggtcaac ttcatggcgc tgtcgacgac gggagggttc
     8221 cacaccgcga tcgcgaacct gcggcacggc caggctcacc gcgcggtcga tcgcatcgat
     8281 gccgacacga tgcgcttcgt ggccgccgaa ggtgtgcagg tgctgtgcgg ctcgccccag
     8341 cagatggcgc acgcggtcgg cgtgctggac gaggcccgca tcacgctgcc gcagctggag
     8401 gaggtgcgta tggctggggc gacccccggc gacgcgctcc tgcgccgcat cgccgaggtg
     8461 ctggcggtgc cggtgcgcag cgtctacggg tcgaccgagg gaggcggggt cactatgcgt
     8521 atgctcgccg ccggagacga tctcgcgaac gtcggaccag cccttcaggg atccgagctc
     8581 gaggtcgtcg acgagaacgg cgcaccccta cccctcgggg tcgagggcac tgtgcgctac
     8641 cgaagccccg gccagacctc cggctacctg gtgggtgaca ccgtcgagcc gttccccggc
     8701 gggtggttcg cgccgggcga cctcgggatg ctcgaacccg atggctcgct ggtgctcgcg
     8761 ggccgctcgg ccgagattct caacatcgcg ggtcagaagg tggaccccgc ggttgtcgac
     8821 gggctggccg cgcaattccc cggggttcgg gatgccgcgg ccttcggttt cgagcgcccc
     8881 acggggctcg ccgaactcgg cctcgccgtg gttgcggacg cggagtgcga cctgcgcgcc
     8941 ctcgaccgcg acctgcgtgc ccggctgccc ggcgcccacc cgacgacgtt ctggcgggtc
     9001 tccgagatcc cccgcaatcg catgggcaag gccgaacgag gcgtcctggc ggaggccttc
     9061 gggcgaaccg gctcggtgcg ctagccgacg gccttcgtca cgagctgggc gatcagcgtc
     9121 tcgtcggcag gcgtcaggtc cttgatggcg aacgcgaccg gccacatgct gccgtcgtcg
     9181 agcgtggcgg cctcgttgaa accgagggtg gcgtaccgca caccgaactt ggcggcgctc
     9241 tggaagaagc agacgatctg gtcgcccttg gcgtaggcgg gctggccgta ccaggtcttg
     9301 ggcgtgagct cgggggcggt ggtcgtcacg atccggtgga tcgcctccgc gatgacgcgg
     9361 tcgtgctcgg gcatctcagc gatcttggca agcaagtcct tgagcaggtc ctgcgccttg
     9421 gcctgggcct tgagttcctt ggcgcgctcc ttcatggcgg cgcgttcctc tgcgctgaat
     9481 gactcggtgg gtgacataag cgactcctct gtgcggtggc gggaacatcc ccagcctaag
     9541 gagggtccct cgaatgcgct tctccgatcg tgctcactac tgcaactcat gggttgcatt
     9601 aggcctggcg accgagcatc atggagtcat gaccgacaac cccacatcca ccgtcgacga
     9661 gaccacgttc agcgtgcgcc gcaccatcac catcgccgcc cccattgagt cggtctgggc
     9721 cgccgtcacc gaggcacagc acatctcgaa gtggttcggc gaggcggcac tcaacggcag
     9781 cggcgtcggc gcactcggca ccctgagctg ggacgactac ggcacgatcc ccatccgcgt
     9841 cgaagcgatg gatgccccca cgctcgtgtc ctatcgctgg ggcaacgacg acgcgagcgg
     9901 tgtgctcgcc gaccagatcg acgacgacca ctcgactgtc ttcaccttca cgctcgagcc
     9961 gatcgagacg ggcacacgcc tcacggtcgt cgagaccgga ttcgacacca cgtccgatcc
    10021 gatcgccaac ctggagagcc accgcgaggg ctgggactcc gaactcgacg agctggtcgc
    10081 cctgctggag gccggcctgt gagcgaggtg ctggtgccgg tgttcgccgc cctcggggat
    10141 gacacgcgct ggagcatcct cgccgccctc ggtcagggcg acgcctcggc ctcggggctc
    10201 gcgtcgcggc tgccagtctc gcggcaggcc atcgccaagc acctcacgat cctcgaagag
    10261 gtgggactcg tcgaaccgct ccgtgtcggc cgggaggtgc gctaccgcgt cgtcggcgcc
    10321 cagctcgagg cgacggcgcg gcgtctcgac gagctcggct tcgcgtggga tcgccgactg
    10381 gcggcgatca aggagatcgc ggagcggctg tgatgacaac atcgcacgtg atcaagcccc
    10441 tcacgcccga gaccttcccg gcctggctgg ccctcgcgca gaaacacaac ggcgtctggg
    10501 gcggatgcta ctgctcctac ttccactcag acaccgcgca gacggtcaag agcgagtacg
    10561 accgcgcgac cttcaaggag cggctggtcg ctgagggcgt cgcgcacgcg gcgctcgtgt
    10621 tcgatggtga caccgcgatc gcgtggtgcg agtacggcag cccactcgag cttccgggca
    10681 tctaccaccg caagcagtac gacgcgggcg agaccaagcc ggcgccgtgg cgcatcacct
    10741 gcttcttcgt cgaccgcgac caccggcggt cgggcgtcgc ccgagaggct ctggatggcg
    10801 cgctcgagct gatcgcacaa gccggcggcg gcgaggtggt gtcgtttccc aacgagctcg
    10861 cgccgggcaa gcgcacgtcg tcgtcgttcc tccacaacgg cacgagggcc atgttcgaga
    10921 aggc
//
LOCUS       DAOSOU010000052        10848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6050_length_10848_cov_7.052997, whole genome shotgun sequence.
ACCESSION   DAOSOU010000052 DAOSOU010000000
VERSION     DAOSOU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_10848_cov_7.052997"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLW15_04460"
     CDS             complement(<1..988)
                     /locus_tag="PLW15_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOT33686.1"
                     /translation="MTDDTAPAAELTEAEVSEQKAVRLEKRQRLIDSGAGAYPVGVGV
                     TTTIPALRAKYPELEADATTGDIVGIAGRVVHLRNTGKLCFASLQSGDGQRIQAMISL
                     AEVGEESLERWKDLVDLGDHVFVSGEVISSRRGELSVLARDWAIAAKAILPLPNLHSE
                     LSDEQRVRSRYLDLIVREQARFTVRARAAVNASLRSTFSAHEYLEVETPMLQVQHGGA
                     SARPFITHSNAFDTELYLRIAPELFLKRAVVGGIDRVYEINRNFRNEGADSTHSPEFA
                     MLEAYEAYGDYNQMADLTQELVQNAARAVSGGLVVTWADGTEYDLGGQWDRIS"
     gene            complement(1048..1242)
                     /locus_tag="PLW15_04465"
     CDS             complement(1048..1242)
                     /locus_tag="PLW15_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33687.1"
                     /translation="MIELVEIPDGLDELDQIPTVLTSSSRSPTVSTSSTTDPATLADQ
                     EIAEKRRSLDAAPRRVAQIS"
     gene            complement(1245..2093)
                     /gene="panC"
                     /locus_tag="PLW15_04470"
     CDS             complement(1245..2093)
                     /gene="panC"
                     /locus_tag="PLW15_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOT33688.1"
                     /translation="MPVTVAATVVELRSLIGGRRVALVPTMGALHDGHLALAARAVEL
                     VSQGVVDVVAASIFVNPLQFGPNEDLEKYPRTFDDDVAKLDAAGIDFVFAPTVEEMYP
                     NGPLETKVTAGTVGSMFEGRSRPGHFDGVLTVVNKLLNIAGASAVLFGQKDAQQAFLV
                     QRMVRDLSMPVRVEIVETVREESGLALSSRNRFLDAKEHRAARVLSTALEAAASSADR
                     GIDAVIAAAQSVLMGENLVELDYLAVVNPATFLPVDDDYRGPARVLIAARVGSTRLID
                     NERIRL"
     gene            complement(2117..3166)
                     /locus_tag="PLW15_04475"
     CDS             complement(2117..3166)
                     /locus_tag="PLW15_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33689.1"
                     /translation="MSLFEGIIARTVPTSRLKVNVLERAGQATDGPAIVLVHGNVSSS
                     LFWQEVMLQLPADLPVYAIDLRGFGDSETAPVDATRGLRDFSDDVAATIDELGLGAVH
                     LVGWSMGGGVAMQYALDHPVLSLTLQAPVSPYGFGGTRLDGTRLTDDDAGVGGGGANP
                     AFVASITEQDAGADNPMASRAVFRSAYVAAGYTSDHEDTWVESMLTTKTGDANYPGTA
                     AASENWPGFAAGDRGVLNTMAPKYFNVSGIVDLAVKPPVLWIRGDSDAIVADATFFDI
                     NYLGQAGVIPGWPGEDVAPAQPMISQTRAVFEKYAANGGSYTELVFENCGHSPHLEYP
                     DKFVTALVEHVRAAG"
     gene            3249..>4116
                     /locus_tag="PLW15_04480"
     CDS             3249..>4116
                     /locus_tag="PLW15_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT33690.1"
                     /translation="MNFPANRDFDAPVVGGLLHGGIWGADASPPVLAIHGITASHLAW
                     ATVARALPEHRLIAPDLRGRGASATLPGPWGMARHADDVVAALDHLGIEKAQVVGHSM
                     GAFVAAVVARRHPDRVSSLLLVDGGLPLELPPGVRLDDLERTLGPAAERLGMTFESPE
                     AYRAFWRSHPAFAEHAPAELDAYVDYDLASVAGELKSRTSADAMRYDSRELYGGDVVV
                     DALEALGGAILLTAPRGLQNEKPGLYPPEVLARWQQRVPELTILEVEHVNHYTIVMGE
                     RGAATVAGVIRSQ"
     assembly_gap    4117..4216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4217..4911)
                     /locus_tag="PLW15_04485"
     CDS             complement(<4217..4911)
                     /locus_tag="PLW15_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOT33691.1"
                     /translation="MSTPSGRLGVGIVGAGRVGPVLGAALAGAGHAIVGISAVSEASR
                     DRAEAMLPGVPVLEVPDLIERSELVILAVPESELQSLVAGLAATGAWQPGQLVLHTAP
                     GVGTSVLAPAMAAGAIPLAINPAMVFTGTSIDLVRLRESFCAVTAPTPVQPIGQALVV
                     EMGAEPILVAEADRPAWAEAVSTATSFSSAIVGQAVGLLGGIGIESPGSVLGPLVRSS
                     VETALQNAVDPGLG"
     gene            complement(4908..6479)
                     /locus_tag="PLW15_04490"
     CDS             complement(4908..6479)
                     /locus_tag="PLW15_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT33692.1"
                     /translation="MTPAPVTPALGAAGRLADGEWHRLHPATPLLRGGFALVAILGVI
                     LVNLRDVWIDLIIGGPDGGPPDPLVWILEHDYALPVLAAIAAGLILFILGFYVSWRMH
                     TFRITDELVEVRSGILFRTNRRGRLDRIQGINIARPFLARLVGAAKLEINVAGQDANV
                     QLAYLGSAAADELRREILALASGVRAEASAARGGSGLDTLDQRRSTSGFVEQRVTELL
                     APELDPDAAAPESVVQMHLGRLLGSLLLSGTTVFLLVGAAVVATIITLTGEFFILFAV
                     VPGLIGVASFYFTRFTRSLRYSIAATPDGVRVGFGLLSISNETLPPGRIHAVSVSQPL
                     LWRASGWWEIRINRAANSSARGAAGQANTTLLPVGNLTDVRRVLELVLVDPALIELVE
                     RGMVSKGGDDGFVNSPRRAAVVRWFSWRRNGFALAPSAVLLRRGAIWRELVIVPQART
                     QSITLNQGPLARRLRLAALTVQTVTGPVSARLGAIDAESAVGLFGDVADAAIRSATND
                     TSHRWRESRPGSATT"
     gene            complement(6509..6991)
                     /locus_tag="PLW15_04495"
     CDS             complement(6509..6991)
                     /locus_tag="PLW15_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT33693.1"
                     /translation="MSRLELDVTEWRRVSPKYVWVDLVGMLIAAIIVVGATSLPAVFT
                     HNVYLFTIPASLAIIFIIALALTPRRVRAIGYQLRSDDLLFRKGLMFQRFVSVPYGRM
                     QLVDINRGPLDRALGLSELKFVTAAAATGVVIPGLPEADAETLRDELVALAESRRAGL
                     "
     gene            complement(6988..7470)
                     /locus_tag="PLW15_04500"
     CDS             complement(6988..7470)
                     /locus_tag="PLW15_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HOT33694.1"
                     /translation="MSRTKPLHLVLLAAFGAVAAWLLETVLAASGRAVLLPPVTLAIA
                     LVLIGAIVVIMALPVRRVSRRVEGAKVDPFYATRVVMLAKASSLSGALLGGAGLGIAF
                     YLLGRAVLPGVGSITMAFACAAGAILLLVAGLIAEHMCTIPPDDDPKNDGRPDPRGIS
                     "
     gene            complement(7467..7973)
                     /gene="folK"
                     /locus_tag="PLW15_04505"
     CDS             complement(7467..7973)
                     /gene="folK"
                     /locus_tag="PLW15_04505"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOT33695.1"
                     /translation="MEFPAVIALGSNLGDREATLRAAVADIAAIDGVVLLRASSLYET
                     PALKPEGIDTDAPAYLNAVVAVRSALDPHALLAALHGIENAHGRVRDVRWGDRTLDLD
                     LVSFGGQSMDSADLTLPHPRAHERAFVLAPWLEVEPAAMVPGRGSVRALLDATGESAS
                     VYPAEPLL"
     gene            complement(7994..8392)
                     /gene="folB"
                     /locus_tag="PLW15_04510"
     CDS             complement(7994..8392)
                     /gene="folB"
                     /locus_tag="PLW15_04510"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOT33696.1"
                     /translation="MNPLDEITLTGLRATAFHGVLDHERRDGQVFVIDVTVMLPLGDA
                     ATTDDLARTIHYGELAEEVVAAVESNPVDLIETVAERIATVVLAHERAMFTRVTVHKP
                     HAPISVPFDDVSVTITRGRPERLTPTGFSL"
     gene            complement(8389..9189)
                     /gene="folP"
                     /locus_tag="PLW15_04515"
     CDS             complement(8389..9189)
                     /gene="folP"
                     /locus_tag="PLW15_04515"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT33697.1"
                     /translation="MSVQLLGILNVTPDSFSDGGLYAEQSAAVERGIAMRDEGATLVD
                     VGGESTRPGAERIDPEEEQRRVIPVVRELVAAGVSVSVDTMNAETALAAAEAGASVIN
                     DVSGGLADPGMYRAVAETGLTYIAMHWRGHSTDMDALATYDDVVIDVREELKARLSEM
                     IVSGVDLRRVILDPGLGFAKRPEHNWQLLGRLDELASLGHPLLVGASRKRFLSPWGAE
                     PAERDEATAIVSALAANAGVWGVRVHDVASTRAALEITEAWQNGRTDS"
     gene            complement(9186..9749)
                     /gene="folE"
                     /locus_tag="PLW15_04520"
     CDS             complement(9186..9749)
                     /gene="folE"
                     /locus_tag="PLW15_04520"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOT33698.1"
                     /translation="MAIDSGRIEAAVAEILAAIGEDPSRPGLVDTPRRVAEAYADFFS
                     GVGADPLAVLSDALEGESGELVIVRDIQFRSICEHHLLPFLGVAHVAYAPGARIVGLG
                     RLAELVTLLASRPQLQERLTDEIADALTTALAPEGVVVVLDAVHGCVSARGPRQATSS
                     TVTVASRGALAEPTARAGVLSLIGGAS"
     gene            complement(9757..>10848)
                     /locus_tag="PLW15_04525"
     CDS             complement(9757..>10848)
                     /locus_tag="PLW15_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT33699.1"
                     /translation="IAATNRPDVLDPALLRPGRFDRQIGVDSPDLQGRRQILEVHAKG
                     KPMADGVDLEVVARKTPGFTGADLANVLNEAALLTARSNAQLIDNRALDEAIDRVMAG
                     PQRRTRLMNDKEKLITAYHEGGHALAAASMRNTDPVTKVTILPRGRALGYTMVLPLED
                     KYSVTRNELLDQLTYAMGGRVAEEIVFHDPTTGASNDIEKATAIARRMVTEYGMSTKV
                     GSVKLGQAAGEPFLGRDMGYTRDFSDTMAETIDEEVRKLIEQAHDEAWQVLNANRDVL
                     DKLATELLTKETLDHVQLAKIFEGVEKLPERPVWLSSDSRPLSDRPPVKVPLKAPIDA
                     GLSEGGIESEPTKPKRTTRPRKTPGIATA"
BASE COUNT         1636 a         3687 c         3831 g         1594 t
ORIGIN      
        1 tcgagatgcg gtcccactgc ccgccgaggt cgtactcggt gccgtcggcc caggtgacga
       61 cgagtccgcc ggacacggcg cgggccgcgt tctgcacgag ctcctgggtg aggtcggcca
      121 tctggttgta gtcgccgtac gcctcgtagg cctcgagcat cgcgaactcg ggcgagtgcg
      181 tggagtcggc accctcgttg cggaagttgc ggttgatctc gtacacccgg tcgatgccgc
      241 cgaccaccgc gcgcttgagg aacagctcgg gcgcgatgcg caggtacagc tcggtgtcga
      301 aggcgttgct gtgggtgatg aagggcctcg cggatgcccc gccgtgctgc acctgcagca
      361 tcggggtctc gacctcgagg tactcgtgcg cggagaacgt cgagcgtagg ctcgcgttca
      421 ccgcggccct agcgcgcacc gtgaagcgcg cctgctcgcg aacgatcaag tcgaggtagc
      481 ggctgcgcac gcgctgctca tcgctgagct cggagtgcag gttcggcagc ggcaggatcg
      541 ccttcgcggc gatcgcccag tcgcgcgcga gcaccgacag ctcgccacgg cgactcgaga
      601 tgacctcacc cgagacgaac acgtggtcgc cgaggtcgac gaggtccttc cagcgctcga
      661 gggactcctc gcccacctcg gcgagcgaga tcatggcctg gatgcgctgt ccgtcacccg
      721 actgcaggct cgcgaaacac agcttgcccg tgttgcgcag gtgcaccacg cggccggcga
      781 tgccgacgat gtcgccggtg gtggcatccg cttcgagctc ggggtacttc gcccgcaggg
      841 cgggaattgt cgtggtgacg cccacgccga ccgggtacgc gccggcgccc gaatcgatga
      901 gccgctgacg cttctccagc cgcacggcct tctgctcgga gacttcagcc tcggtcagtt
      961 cggcggcggg tgcggtgtcg tcggtcacga gggtcaatcg taccggggcg acgtcggagc
     1021 tcttccccct ccgccctggt tcgccgatca ggagatttgc gcgacacgcc gcggtgcggc
     1081 atccagcgac cggcgtttct ccgcgatctc ctgatcggcg agcgtagcgg ggtcggtggt
     1141 cgagcttgtc gagaccgtcg gggatctcga cgagcttgtc aagaccgtcg ggatctggtc
     1201 gagctcgtcg agaccgtcgg ggatctcgac gagctcgatc accgctagag gcggatgcgc
     1261 tcgttgtcga tgaggcgggt cgagcccacg cgcgcggcga tcagcacgcg ggcggggccg
     1321 cggtagtcgt catcgacggg caggaacgtg gccgggttga ccacggcgag gtagtcgagc
     1381 tccacgaggt tctcccccat gagcaccgac tgtgctgcgg cgatcacggc atcgatgccc
     1441 cgatcggcgg atgacgctgc cgcctccaac gccgtcgaca gcacccgcgc tgcccggtgc
     1501 tccttcgcgt cgaggaaccg gttgcggctg gatagcgcga gccccgactc ctcccgcacg
     1561 gtctcgacga tctccacccg cacgggcatg ctgaggtcgc gaaccatgcg ctgcacgagg
     1621 aacgcctgct gggcatcctt ctggccgaac agcacggcgg aggcgccggc gatgttcagc
     1681 agcttgttga cgacggtgag cacgccgtcg aagtgcccgg gccgcgagcg cccctcgaac
     1741 atcgacccga cggtgccggc cgtgaccttg gtctcgagcg ggccgttcgg gtacatctcc
     1801 tcgacggtcg gagcgaagac gaagtcgatt cccgcggcgt cgagtttcgc gacgtcgtca
     1861 tcgaacgtgc gcggatactt ctcgaggtct tcgttcggcc cgaactgcag ggggttcacg
     1921 aagatggatg ccgcgacaac atccacgacg ccctggctca cgagctcgac ggctcgcgcg
     1981 gccagcgcca gatgcccgtc gtgaagcgcg cccatggtgg ggaccagcgc aacccgccgc
     2041 ccaccgatca ggcttcgaag ctcgacgacg gtggctgcta cggtgacggg cacctgtcga
     2101 tagtaccggg ggtcgatcag cccgccgcgc gcacgtgctc cacgagtgcg gtcacgaact
     2161 tgtcggggta ctcgaggtgc ggcgagtggc cgcagttctc gaagacgagc tcggtgtagc
     2221 tgccgccgtt ggccgcatac ttctcgaaca ccgcgcgggt ctgggagatc atcggctgcg
     2281 ctggggcgac atcctcgccc ggccagccgg ggatgactcc ggcctgaccg aggtagttga
     2341 tgtcgaagaa ggtggcgtcg gcgacgatcg cgtcggagtc gccgcggatc cacagcacgg
     2401 gcggcttgac ggcgaggtcg acgattcccg agacgttgaa gtacttcggt gccatggtgt
     2461 tgagcacgcc ccggtcgccc gcggcgaagc ccggccagtt ctcggatgcc gcggcggtgc
     2521 cggggtagtt cgcgtcgccg gtcttcgtgg tgagcatgga ctcgacccag gtgtcctcgt
     2581 ggtcgctcgt gtacccggcg gcaacgtagg cgctgcggaa cacggcgcgc gaggccatcg
     2641 ggttgtcggc gcccgcatcc tgttcggtga tgctggccac gaacgcgggg ttggcgccac
     2701 cgccgccgac acccgcgtca tcgtcggtca agcgggtgcc gtcgaggcgg gttccaccga
     2761 agccgtaggg cgagaccggc gcctgcaggg tgaggctgag cacggggtgg tcgagcgcgt
     2821 actgcatcgc cacgccgccg cccatcgacc aaccgacgag gtgcacggca ccgaggccga
     2881 gctcgtcgat ggtcgcggcg acgtcgtcgc tgaagtcgcg aagcccgcgg gtcgcgtcga
     2941 cgggggccgt ctcgctgtca ccgaagccgc gcaggtcgat cgcgtacacc ggcaggtcgg
     3001 cgggcagctg cagcatgacc tcctgccaga acagcgacga cgagacattg ccgtgcacga
     3061 gcacgatcgc gggaccgtcg gtcgcctgtc cggcccgctc gagcacgttg accttcagcc
     3121 gtgatgtcgg cacagtgcgt gcgatgattc cttcgaagag cgacattgct ggttcttcct
     3181 ttcacgacgg tgtgacggtg atactgtaac tgtaacctga atcgtctctc aggttggagg
     3241 agatcttcat gaatttcccc gcgaatcgtg acttcgacgc accggtcgtc ggaggtctcc
     3301 tccacggcgg catctggggc gcggatgcct cgccccccgt tctcgcgatc cacggcatca
     3361 cggcctccca cctcgcctgg gccaccgtcg cccgcgcgct gcccgaacat cgcctgatcg
     3421 cgccggacct ccgtggacgc ggcgcaagcg cgaccctccc ggggccgtgg ggcatggccc
     3481 ggcacgccga cgacgtcgtc gccgcgctcg accacctcgg tattgagaag gcacaggtcg
     3541 tggggcactc aatgggggcg ttcgtcgccg cggtcgtggc ccgcaggcat ccggatcgcg
     3601 tctccagcct gctgctcgtg gatggcggcc tgccgctcga gctgccgccg ggggtgcggc
     3661 tcgacgacct cgagcgcacg ctcggcccgg cggcggagcg cctcgggatg accttcgaga
     3721 gccccgaggc gtaccgcgcc ttctggcgca gccacccggc gttcgccgag cacgcccccg
     3781 ccgaactcga cgcctacgtc gactacgacc tggccagcgt cgccggcgag ctgaagtcgc
     3841 gcacctcggc cgacgcgatg cgctacgact cccgcgaact gtacggcggc gacgtcgtcg
     3901 tcgatgcgct ggaggcgctc ggtggggcga tcctgctcac cgcgccgcgc ggactccaga
     3961 acgagaagcc cgggctctac ccgccggagg tgctcgcccg ctggcagcaa cgagtgccgg
     4021 agttgacgat cctcgaagtc gagcacgtca accactacac gatcgtgatg ggtgagcgcg
     4081 gggccgcaac ggtggccggc gtcatccgct cgcagtnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnccga gccccgggtc gacggcgttc tgcagcgccg tctcgacgga
     4261 tgaccgcacc aaaggtccca gcaccgagcc gggtgactcg atgccgatgc cgccgagcag
     4321 ccccaccgcc tggccgacga tcgcgcttga gaagctcgtc gccgtggaca ccgcctcggc
     4381 ccacgccggt cggtcggcct cggcgacgag gatcggctcc gcgcccatct cgacgacgag
     4441 ggcctggccg atgggctgca ccggcgtcgg cgcggtgacc gcgcagaagc tctcgcgcag
     4501 gcgcacgagg tcgatgctcg ttccggtgaa caccatggcc gggttgatcg cgagcgggat
     4561 agcaccagcc gccatcgcag gtgccagtac cgacgttccg accccgggcg cggtatgcag
     4621 caccagctgg cccggttgcc acgctcccgt cgcggcgagg ccggcgacca gcgactggag
     4681 ctccgactcc ggcaccgcga gaatcaccag ttcgctgcgt tcgatcaggt cgggcacctc
     4741 gagcacggga acccccggca gcatcgcctc ggcccgatcc cgactcgcct cagagaccgc
     4801 gctgatgccg acgatcgcgt gccccgcgcc agcgagcgcg gcaccgagca cggggccgac
     4861 gcgaccggca cccacgatcc ccaccccgag gcggcccgag ggggtgctca cgtggtggcc
     4921 gagccggggc gggactcgcg ccagcggtga ctcgtgtcgt tggtggcgct ccggatggcg
     4981 gcatccgcta cgtcaccgaa caggcccacc gcgctctcgg catcgatggc accgagccga
     5041 gccgagacgg ggcctgtgac ggtctgcacc gtgagcgcgg ccagacgcag tcgacgggcc
     5101 agcggtccct gattcagggt gatgctctgg gtgcgggcct gcggaacgat gacgagctcg
     5161 cgccagatcg cgccgcggcg cagcagcacc gcagacggcg ccagcgcgaa gccgttgcgc
     5221 cgccacgaga accagcgcac cactgcggcg cgacggggcg agttcacgaa gccgtcgtcg
     5281 ccgcccttcg acaccatgcc gcgctccacg agctcgatca gcgcggggtc gacgagcacg
     5341 agttcgagca cccggcgcac gtcggtgagg ttgccgaccg gcagcagcgt ggtgttcgcc
     5401 tgcccggcgg caccgcgcgc cgagctgttg gcggcgcgat tgatgcggat ctcccaccag
     5461 cccgacgctc gccagagcag gggctggctc accgagacgg cgtggatgcg ccccggcggc
     5521 agggtctcgt tgctgatcga cagcaggccg aagcccaccc gcacgccgtc gggcgtcgcc
     5581 gcgatgctgt accgcagcga ccgcgtgaac cgggtgaagt agaagctcgc aacaccgatc
     5641 agacccggca cgacggcgaa caggatgaag aactcgccgg tcagcgtgat gatcgtcgcg
     5701 accacggccg cgcccaccag caggaacacg gtcgttccgc tgagcaggag cgagcccagc
     5761 aggcgaccga ggtgcatctg cacgacggat tcgggagccg cggcatccgg atcgagctcg
     5821 ggcgcgagca gctcggtgac ccgctgctcg acgaatccgg atgtcgaccg ccgctggtcg
     5881 agcgtgtcga gacccgaccc gcctctagca gcggatgcct cggcccgcac ccccgacgcg
     5941 agcgcgagta tctcccgccg aagttcgtca gcggccgccg aaccgagata ggcgagttgc
     6001 acgttggcgt cctgcccggc cacgttgatc tccagcttcg cggctcccac gagcctcgcc
     6061 aggaacgggc gggcgatgtt gatgccctgg atgcggtcga gccgcccccg acggttggtg
     6121 cggaacagga tgccgctgcg cacctccacg agttcgtcgg tgatgcggaa cgtgtgcatg
     6181 cgccacgaca cgtagaaacc gaggatgaag aggatgagac cggcggcgat cgccgcgagc
     6241 acgggcagtg cgtagtcgtg ttcgaggatc cagacgagag ggtcgggcgg gccgccgtcg
     6301 gggccgccga tgatgaggtc aatccagacg tcgcgcaggt tcacgaggat cacgccgagg
     6361 atcgccacga gcgcgaagcc gccgcgcagc agcggggtcg cggggtgcag tcggtgccac
     6421 tcgccgtcgg cgagccgccc ggcagcgccg agcgccgggg tcacaggcgc cggcgtcacg
     6481 ggtggcgggg tcgcgccagc cgcctcgctc atagtccggc ccggcggctc tcggccagcg
     6541 ccacgagctc gtcgcgaagc gtctcggcgt cggcctcggg cagccccggg atcaccactc
     6601 ctgtcgcggc cgcagcggtg acgaacttga gttcgctgag tcccagcgcc cggtcgagcg
     6661 gaccgcggtt gatgtcgacc agctgcatgc gcccgtacgg caccgacacg aagcgctgga
     6721 acatcagccc cttgcggaag agcaggtcgt cggagcgcag ctggtagccg atcgctcgca
     6781 cccggcgggg cgtgagcgcg agggcgatga tgaagatgat ggcgagcgac gcggggatcg
     6841 tgaacaggta cacgttgtgg gtgaagacgg cgggcaggct cgtcgcgccc accacgatga
     6901 tggcggcgat gagcatgccg acgaggtcga cccacacgta cttgggcgag acccgtcgcc
     6961 actcggtgac gtcgagttcg agtcggctca cgagattcct ctggggtcgg gacgaccgtc
     7021 gttcttcggg tcgtcgtccg gcgggatggt gcacatgtgc tcggcgatca ggcccgccac
     7081 gagcagcagg atcgctccgg cggcgcaggc gaacgccatc gtgatggagc ctaccccggg
     7141 cagcaccgcg cgcccgagga ggtagaaggc gatgcccaga cccgcgccgc cgagcagcgc
     7201 cccgctgagg ctcgacgcct tggcgagcat cacgaccctc gtcgcgtaga acgggtcgac
     7261 cttcgcgccc tccacgcgcc gcgagacgcg ccgcaccggc agcgccatga tcacgacgat
     7321 cgcgccgatg agcacgagcg cgatcgcgag ggtcaccggg ggcagcagaa ccgcccgacc
     7381 ggatgccgcg agcacagtct cgagcaacca tgcggccacc gctccgaacg cagccaacag
     7441 caccaggtgc aacggcttgg tgcggctcac agcagcggct ccgcgggata gaccgacgcc
     7501 gactcaccgg tggcgtcaag gagcgcgcgc acgctaccgc gaccgggcac catcgcggcg
     7561 ggttcgacct cgagccaggg tgcgagcacg aacgcgcgtt cgtgcgcccg ggggtggggc
     7621 agcgtgaggt cggcgctgtc catgctctgg ccgccgaagc tcacgaggtc gaggtcgagg
     7681 gtgcgatccc cccagcgcac gtcgcgcacc cggccgtggg cgttctcgat gccgtggagc
     7741 gcggcgagca gcgcgtgggg gtcgagggcc gagcgcacgg cgacgaccgc gttgaggtag
     7801 gccggggcat ccgtgtcgat gccctcgggc ttgagagcgg gggtctcgta gaggctcgat
     7861 gctcgcagca gcacgacgcc gtcgatcgcc gcgatgtcgg cgacggcggc gcgcagggtg
     7921 gcctcgcggt cgccgagatt gctgccgaga gcgatgaccg cgggaaactc cacgcgcaga
     7981 cgttgggctc gaatcacagc gagaatcccg tgggggtgag ccgctcgggg cgtccgcggg
     8041 tgatcgtgac ggatacgtcg tcgaacggca cggagatcgg cgcgtggggc ttgtgcactg
     8101 tcaccctggt gaacatggcg cgctcgtgtg cgagcacgac cgtggcgatg cgttcggcga
     8161 cggtctcgat gaggtcgacc ggattgctct cgacggccgc cacgacctcc tcggcgagtt
     8221 cgccgtagtg gatggtgcgc gcgaggtcgt cggtcgtcgc ggcatccccg agcggcagca
     8281 tcaccgtcac gtcgatcacg aagacctgcc cgtcgcggcg ctcatggtcg agcacaccgt
     8341 ggaacgcggt cgcacgcagc ccggtgaggg tgatctcgtc gagggggttc atgagtcggt
     8401 tcgtccgttc tgccaggcct cggtgatctc gagcgccgcc ctggtgctcg cgacgtcgtg
     8461 cacgcgcact ccccacacgc cggcgttcgc cgcgagggcg ctcacgatcg cggtggcctc
     8521 gtcacgctcc gcgggctcgg caccccacgg cgacaggaat cgcttgcgcg aggcgcccac
     8581 gagcagagga tgcccgaggc tggccaactc gtcgagccga cccagcagct gccagttgtg
     8641 ctcggggcgc ttcgcgaagc ccaggcccgg gtcgaggatc acgcggcgca ggtcgacgcc
     8701 cgacacgatc atctcgctca gccgcgcctt gagctcctcg cgcacatcga tgacgacgtc
     8761 atcgtaggtg gcgagtgcat ccatgtcggt ggaatggcca cgccagtgca tggcgatgta
     8821 ggtgagcccg gtctcggcga cggcgcggta cattccgggg tcggccaggc cgcccgagac
     8881 gtcgttgatg acggatgccc cagcctccgc cgcggcgagc gccgtctccg cgttcatggt
     8941 gtcgacgctc acgctcacgc ccgccgccac gagctcgcgc accacgggga tcacccggcg
     9001 ctgctcctcc tcggggtcga tgcgctcggc gccgggccgg gtggactcgc cgccgacatc
     9061 gacgagggtc gcaccctcgt cacgcatcgc gatgccgcgc tcgaccgcgg ccgactgctc
     9121 cgcgtagaga ccgccgtcgc tgaacgaatc gggcgtgacg ttgaggatgc cgagcagctg
     9181 cacgctcatg aggcaccccc gatcagcgag agcacgccgg cccgagcggt cggctccgcg
     9241 agggcgcccc gggatgcgac ggtcaccgtc gaactcgtgg cctgcctcgg tccgcgtgcc
     9301 gagacacacc cgtgcaccgc gtcgagcacc acgacaacac cctcaggggc gagggcggtg
     9361 gtcagggcat ccgcgatctc gtcggtcagg cgttcctgga gctgggggcg cgaggccagc
     9421 agtgtcacca gctcggccag ccgaccgagg ccgacgatgc gcgcacccgg cgcgtaggcg
     9481 acgtgcgcaa caccgaggaa cggcagaagg tgatgctcgc agatcgaacg gaactggatg
     9541 tcacgcacga tcacgagctc gcccgactcc ccctcgagcg catcggagag aaccgccagc
     9601 gggtcggccc cgacccccga gaagaagtcg gcgtacgcct ccgcgacccg acgcggggta
     9661 tcgacgaggc ccggtcggct cggatcctca ccgattgcgg cgaggatctc ggccacggcc
     9721 gcctcgatgc gcccggagtc gatggccatc ggcacctcag gcggtggcga tgccgggagt
     9781 cttgcgcgga cgggtggtgc gcttcggctt cgtcggctcc gactcgatgc caccctcgga
     9841 caggccggcg tcgatcggtg ccttgagcgg caccttcacg ggcgggcggt ccgacagggg
     9901 acggctgtcg ctcgacagcc acaccggacg ctcgggcagc ttctcgacgc cctcgaagat
     9961 cttcgccaac tgcacgtggt cgagggtctc cttggtgagc agctcggtcg ccagcttgtc
    10021 gagcacgtcg cggttggcgt tgagcacctg ccacgcctcg tcgtgggcct gctcgatgag
    10081 cttgcgcacc tcctcgtcga tggtctcggc catcgtgtcg gagaagtcgc gggtgtaacc
    10141 catgtcgcgg ccgaggaacg gctcgccggc cgcctggccg agcttgaccg acccgacctt
    10201 cgtgctcatg ccgtactcgg tgaccatgcg gcgagcgatc gccgtggcct tctcgatgtc
    10261 attggatgcc ccggtcgtcg ggtcgtggaa cacgatctcc tcggcgactc gcccacccat
    10321 cgcgtaggtc agctggtcga gcagctcgtt gcgggtgacc gagtacttgt cctcgagcgg
    10381 cagcaccatc gtgtagccga gggcgcgacc gcgcggcagg atcgtgacct tggtcaccgg
    10441 gtcggtgttg cgcatgctcg ccgcggcgag ggcgtggcca ccctcgtggt acgccgtgat
    10501 gagcttctcc ttgtcgttca tcagacgcgt gcgacgctgc gggccggcca tgacgcggtc
    10561 gatggcctcg tcgagcgcac ggttgtcgat gagctgcgcg ttgctgcggg cggtgagcag
    10621 cgcggcctcg ttgagcacgt tggcgaggtc ggcacccgtg aagccgggcg tcttgcgggc
    10681 gaccacttcg aggtcgacgc catccgccat cggcttgccc ttggcgtgca cctcgaggat
    10741 ctgccgacga ccctggaggt cgggggagtc cacgccgatc tggcggtcga agcgacccgg
    10801 gcgcagcagc gcggggtcga gcacgtcggg gcggttcgtc gcggcgat
//
LOCUS       DAOSOU010000053        10513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6318_length_10513_cov_5.853031, whole genome shotgun sequence.
ACCESSION   DAOSOU010000053 DAOSOU010000000
VERSION     DAOSOU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6318_length_10513_cov_5.853031"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /gene="sucB"
                     /locus_tag="PLW15_04530"
     CDS             <1..1109
                     /gene="sucB"
                     /locus_tag="PLW15_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HOT33700.1"
                     /translation="EPVAEAPAPVVEAPAPAPVVEAQAPAPVVEAPAPAPVVEAPAPV
                     AEAPAPAPAPAPAAPAAAPSAPSADAAGYVTPLVRKLAHESGVDLSAVTGTGVGGRIR
                     KQDVLAATSASVSTAAPVAAPAKALEVSPLRGTTVAMSRLRKVIAERAVISMQSSAQL
                     TSVVEVDVTLVAHYRDQVKAAFQQKTGVKLSFLPFFALAAAEALTAYPIINATIEGDS
                     IVYPAQENISIAVDTERGLLTPVIRDAAKLDLAGFAAQIADLAERTRDNKLKPDELAG
                     GTFTLTNTGSRGALFDTPIVFLPQSAILGTGIVTKKPVVVSDAGGDAIAIRSMVYLAL
                     SYDHRIVDGADAARFLVAVKDRLEGGDFAAKLGI"
     gene            complement(1119..2462)
                     /locus_tag="PLW15_04535"
     CDS             complement(1119..2462)
                     /locus_tag="PLW15_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT33701.1"
                     /translation="MTGDTLGGYRLVRKLGEGPRAEVHLAYPRSDSDDARAVAIKVFR
                     PTVGDTAAITEIEALSRAAGDHVVTLIDLAGGPDGAPALILERVAGGSLGRLLRDRAS
                     LRQGEAITILVPLARTLRRLHDNGVVHGGLRLEAVAFDGTGAPVLSCFGSASLIEPRM
                     PPARLEQEQGVIRDLDDFARVARAVLDRLPLPVSLPDPGQDWLERVEQVLFDLGAPEA
                     VQLRPDGPAELAPPARILTAPPVPPEPEVGGRGGLLSLVLPDWLDGAGLAVSVHRWAS
                     RARRAVLAVRARAWIALGAVAGALVLAAVLIPSSSPDATAGPAPHPSLSPTPTASNAG
                     GDDVVAGDDPLAALPELLALRERCIRDLSVLCLDDVDQPGSAALAEDQLLVRSLQQGS
                     EQPDTLRVSASDLTLEERLGDSALVSLGDVTDSEPASLLMMKSEAGWRIRDYLER"
     gene            2573..3217
                     /gene="lipB"
                     /locus_tag="PLW15_04540"
     CDS             2573..3217
                     /gene="lipB"
                     /locus_tag="PLW15_04540"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOT33702.1"
                     /translation="MIDFVVAGLSANSVPYIEALELQRATHRAVVSGTRPDTVILLEH
                     PSVYTAGKRTEDFERPTDGTPVIDVDRGGKITWHGPGQLVGYPIVRLAEPIDVVGYVR
                     RIEGVLIEVLAELGLDAGRVAGRSGVWMRYDEPVGAEKIAAIGIRVAEGVTMHGFSLN
                     CDNSFDAYSKIVACGILDAGITSVSRELGRDVSPEDVLPLVQKHLESALTGANA"
     gene            3214..4221
                     /gene="lipA"
                     /locus_tag="PLW15_04545"
     CDS             3214..4221
                     /gene="lipA"
                     /locus_tag="PLW15_04545"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOT33703.1"
                     /translation="MSTSHHPEANQVEGRKLLRLEVRNAETPIERKPEWIKTVAKMGP
                     EYRQLQELVKGESLHTVCQEAGCPNIFECWEDREATFLIGGSQCTRRCDFCQIDTGKP
                     ADYDKDEPRRVAESVTKMNLRYATVTGVARDDLPDEGAWLHAETVRQIHAQNPGTGVE
                     ILATDFSGNPDLLGEVFSSRPEVFAHNVETVPRIFKRIRPAFRYERSLDVLTQARAAG
                     LITKSNLILGMGEEPAEVLQALQDLHDAGTDIITITQYLRPTPRHLPVARWVKPQEFV
                     EFSEAAEEIGFLGVLAGPLVRSSYRAGRLWARSMVAKGRDIPEHLTHLADANLGFAQA
                     V"
     gene            4264..4977
                     /locus_tag="PLW15_04550"
     CDS             4264..4977
                     /locus_tag="PLW15_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HOT33704.1"
                     /translation="MARTTTTAPKAPKEPGRLKQMYQVFQMTRRYDKLAIWYLLLAFL
                     LPVLAGIILAIALGGDSILAVVLYVIAGVLGGVLLFLIVLGRRAEKAAYSQIEGQPGA
                     VGAVLKSSLRRGWTASEIPVSVSPKTQDAVYRAVGRGGVALIGEGPRSRTQRMLEDER
                     KSIVRILPNVPVSFIYVGPDAESVPLYKLPTKLRQLKPVLRKPEILAVSNRLNSLGKN
                     GLPIPKGIDPMRVRSQRPR"
     gene            complement(4980..5384)
                     /locus_tag="PLW15_04555"
     CDS             complement(4980..5384)
                     /locus_tag="PLW15_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT33705.1"
                     /translation="MPDPSSAASDWPGRRLGLPASGPRSVGRLGRRIAALVIDWGTAV
                     LVSVAFFQYQAWATLAIFAGVQVLFLLTINASPGHLLLGMRVVPIAGGYLGLWRPFAR
                     TLLLCLVIPALIWDLDQRGMHDRLVGTVLVRR"
     gene            5521..6945
                     /gene="glnA"
                     /locus_tag="PLW15_04560"
     CDS             5521..6945
                     /gene="glnA"
                     /locus_tag="PLW15_04560"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOT33706.1"
                     /translation="MFSDSSEVLKFIKDTDVKFLDIRFTDLPGVQQHFNIPASTVDEE
                     FFSVGQLFDGSSIRGFANIHESDMQLIPDVTTAYVDPFRTERTLIMVFDIYNPRNGEI
                     YAKDPRQVAKKAEKYLASTGIADTAFFAPEAEFYIFDDVRYSVTQNQSFYSVDSEEGA
                     WNTGRVEEGGNLANKTPFKGGYFPVSPVDKQADLRDDISLKLIDAGLILERAHHEVGT
                     GGQAEINYRFDTMVHAADDILKFKYIVKNTALEWGKVATFMPKPLFGDNGSGMHTHQS
                     LWNDGKPLFYDEKGYGGLSDIARWYIGGLLKHAPAVLAFTNPTLNSYRRLVPGFEAPV
                     NLVYSAGNRSASIRIPITGTNPKAKRIEFRAPDASGNPYLAFAAQLMAGLDGIKNKIE
                     PHEPVDKDLYELPPEEAKLIPQVPGSLAEALDALEADHEFLTEGNVFTQDLIDTWINY
                     KRTAEITPMAQRPHPFEYELYFSV"
     gene            complement(7095..10043)
                     /locus_tag="PLW15_04565"
     CDS             complement(7095..10043)
                     /locus_tag="PLW15_04565"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HOT33707.1"
                     /translation="MSRPPTTLTELARLGFTELDETSRRLDLLGAPELVAHFARAADP
                     DQALRYLLGLRESAPVELAALIADPDAAARLIRVLGASSGLGDFLARHPRELQALATP
                     ITAPPTEYGADLLAAVDGLKGDAAWVALRVRYRRHLVQLAAWDLEHADPIAAVSIVAA
                     ALADLSGAALDASLAVARRDVKFPAEEVAATRLAIIGMGKAGARELNYVSDVDVIFVG
                     EAGGLDGESTLADDRAVTIATQLAMQTMRGIHDLATEPGLWEVDANLRPEGKDGALVR
                     TLESHVAYYERWAKSWEFQALLKARPLAGDLELGRQYVEAVSPKVWSSASRENFVDSV
                     QRMRERVTDNIPADELDVQLKLGPGGLRDVEFTIQLLQLVHGQSDPNVRQIATLPALI
                     ALAEHGYIGRVEAAEFSGDYRFLRLLEHRLQLSRLRRTHLMPTDPDALRILARASGIA
                     ANADDLVEQWQRTKQAVRSLHERLFYRPLLSAVAALPEDGLTLSSEQAEARLAAIGFR
                     DPRGALSHIAALTGGVSRRATIQRNLLPVMLQWFADGADPDYGLLAFRRLSEDLGEAY
                     WFLRMLRDSSAAAQRLTQVLSGSKFVGAIFERIPEAAAWLENDDELRPRPYAVLLEES
                     VAAVARHEGDVDAAALALRTARRREILRLALGAILGVVTIEDLGKGLSDITTVILSGA
                     LSLAHHYGDGIEFGIIAMGRYGGAELGFGSDADVMYVYRAAGASDEEAQQRAEAIVRT
                     ISRLTEDMRVPLDLDLGLRPEGRNGAIVRSLESYRAYYERWSLTWEAQALLRARIVVG
                     DRSVLAAWEELVNDVRYPETIAEQDIREIKRIKARVEAERLPQAADPARHLKLGRGSL
                     SDVEWFVQLLQLEHGAKVEGLRTTSTLGALAAAEREGLVTTADAARLHNAWMLASRAR
                     SGMTLWTAKTSDVLPTDRMALEGVARLLEYPPGSATVFEEDYLRTTRLARQVFEKRFY
                     GARLGSATTG"
     gene            complement(10062..>10513)
                     /locus_tag="PLW15_04570"
     CDS             complement(10062..>10513)
                     /locus_tag="PLW15_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOT33708.1"
                     /translation="LWGGDEAPSFVTWGHNNRSALVRVPLYKPNKGQSARVEYRAIDS
                     AANPYLTFSLLLAAGLKGIEEGYELPPEAEDNVWELSDAERRALGYNQLPASLDHAVS
                     LMEDSELVAETLGEQVFSYVLLNKRREWSQYRAQVTPYELESNLEIL"
BASE COUNT         1776 a         3710 c         3429 g         1598 t
ORIGIN      
        1 cggagcccgt ggctgaggca cccgcaccag tcgtcgaggc gcccgcaccc gcacccgtcg
       61 ttgaggccca ggcacccgca ccagtcgtcg aggcacctgc gccggcaccc gttgtcgaag
      121 cgccggcacc cgtcgcagag gctccggcac ccgcacccgc tcccgccccc gctgcgcccg
      181 ccgccgcccc gagcgctccc tcggctgacg cagcaggcta cgtcacaccg ctcgtgcgca
      241 agctcgcaca cgagagcggc gtcgacctgt ccgccgtgac cggaaccgga gtgggtggac
      301 gcatccgcaa gcaggacgtc ctggccgcga catccgcgtc cgtctcgacc gcagcgcccg
      361 tcgccgcacc cgcgaaggct ctcgaggtct cgccgctgcg cggcacgacc gtcgcgatgt
      421 cgcgcctgcg caaggtcatc gccgagcgcg ccgtcatctc gatgcagagc tcagcgcagc
      481 tgacgtccgt cgtcgaggtg gatgtcacgc tcgtggcgca ctaccgcgat caggtcaagg
      541 ctgcgttcca gcagaagacc ggcgtcaagc tgagcttcct gccgttcttc gctctcgcag
      601 cggccgaagc actgacggcg taccccatca tcaacgcgac gatcgagggt gactcgatcg
      661 tctacccggc gcaggagaac atctccatcg ccgtcgatac cgagcgcggt cttctgactc
      721 ccgtgatccg tgatgctgcg aagctcgacc tcgctggatt cgctgcgcag atcgccgacc
      781 tcgccgagcg cacgcgcgac aacaagctga agcccgacga gctggccggg ggcacgttca
      841 ccctgaccaa caccggatcg cgcggcgccc tcttcgacac gcccatcgtg ttcctgccgc
      901 agtcggcgat tctcggcacc ggaatcgtca cgaagaagcc ggtcgtggtg agcgacgcgg
      961 gcggcgacgc catcgccatc cgctccatgg tctatctggc tctgtcctac gatcaccgca
     1021 tcgtcgacgg cgccgacgcc gcccggttcc tggtggctgt caaggatcgc ctcgagggcg
     1081 gcgacttcgc cgccaagctg ggcatctgag cacgcgttct aacgctccag gtagtccctg
     1141 atccgccaac cggcctcgct cttcatcatg aggagcgagg ccggttcgct gtccgttacg
     1201 tcacccagcg acaccagcgc tgagtcgccg agtcgctcct ccagggtcag atccgatgcc
     1261 gagacacgca gggtgtcggg ctgctccgac ccctgctgca gtgacctgac gagcagctgg
     1321 tcctcagcca gcgccgcgga acccggctgg tcgacatcat ccaggcacag caccgacaga
     1381 tcacggatgc accgctcccg cagcgcgaga agctcgggca gtgcggccag cgggtcgtcc
     1441 cccgccacga cgtcgtcgcc cccagcgttg ctcgccgtcg gggtcgggct gaggctcggg
     1501 tgcggagcgg gtcctgcagt cgcatcgggc gacgatgagg gaatcaggac cgcagccagc
     1561 accaaggccc ctgccaccgc cccaagtgcg atccaggcgc gtgcgcgaac cgcgagcacc
     1621 gcgcggcgag cccggctcgc ccaccgatgc accgacaccg ccaggccggc accgtcgagc
     1681 cagtcgggta gcaccaggct caacaggcca cccctccctc cgacctccgg ctcaggcggc
     1741 accgggggcg cggtcaggat tcgggcgggc ggcgcgagtt ccgccgggcc atcgggccga
     1801 agctgcaccg cctcgggcgc cccgagatcg aagagcacct gctcgacccg ctccagccag
     1861 tcctggccgg gatccggcag actgaccggc agtggaagcc ggtcgagcac cgccctcgcg
     1921 acgcgagcga agtcatccag atcgcggatg acgccctgct cctgctccag tcgggccggc
     1981 ggcatccgtg gctcgatcag gctggcagac ccgaagcacg agagcaccgg tgcaccggtt
     2041 ccatcgaacg cgactgcctc cagcctgaga cctccatgca caacgccgtt gtcgtggagg
     2101 cgtctcaggg ttctcgcgag gggaacgagg atcgtgatcg cctctccctg acgcagggac
     2161 gcccggtccc gcaggagcct gccgaggctg ccgccggcca cacgctcaag tatcagcgcg
     2221 ggggcgccat caggtccgcc ggccaggtcg atcagcgtga ccacatgatc gcccgcagcc
     2281 ctcgacagcg cctcgatctc ggtgatggcg gccgtatcgc ccacagtcgg ccggaacacc
     2341 ttgatggcga cggctcgagc atcatcgctg tcgctgcgcg ggtaggcgag gtgaacttcg
     2401 gcacgcggcc cctcgccgag tttgcgaacg agtcgatagc cgccgagggt gtcgccagtc
     2461 atgccaccac cgtgcctcgg attgcggtcg accctgacgc acccgcgact tccgtcgcga
     2521 aacagtgcct ggaggggatg gggaggaccg gaacgcgcat agactcggat gcgtgatcga
     2581 ctttgtcgtc gcggggctaa gcgccaactc cgtgccgtac atcgaagctc ttgagcttca
     2641 acgcgcaacc catcgtgccg tcgtgagcgg aacccgcccc gacaccgtca tcctcctcga
     2701 gcatccgtcg gtctacaccg ccggtaagcg cacggaggac ttcgaacgcc ccaccgacgg
     2761 cacgcccgtc atcgatgtcg accggggtgg caagatcacc tggcacggcc cggggcagct
     2821 ggtcggctac ccgatcgtgc gtctcgccga gcccatcgac gtggtggggt acgtgcgtcg
     2881 catcgagggc gtgctcatcg aggtactcgc cgagctcggc ctcgatgccg ggcgcgtagc
     2941 ggggcgctcg ggcgtctgga tgcgctacga cgaaccggtg ggcgcggaga agatcgcggc
     3001 catcggcatc cgggtcgccg agggagtgac gatgcatggc ttctcactca actgcgacaa
     3061 cagcttcgac gcctactcca agatcgtcgc gtgcggcatt ctcgacgccg gaatcacgtc
     3121 agtctcgcgc gagttgggcc gagacgtctc ccccgaggat gtgctgcccc tcgtgcagaa
     3181 gcacctcgaa tccgccctca caggagccaa cgcatgagca cgagccacca ccccgaagcc
     3241 aatcaggtgg agggccgcaa gctgctgcgc ctcgaggtgc gcaatgccga gaccccgatc
     3301 gagcgcaagc ccgagtggat caagaccgtc gcgaagatgg ggccggagta ccgacagctg
     3361 caggagctcg tcaagggcga gagcctgcac acggtgtgcc aggaagcggg atgcccgaac
     3421 atcttcgagt gctgggagga ccgtgaggcg accttcctca tcggtggcag ccagtgcacg
     3481 cggcggtgcg acttctgcca gatcgacacc ggcaagcccg ccgactacga caaggacgag
     3541 ccacgtcggg ttgccgagtc cgtcaccaag atgaacctgc gctatgccac cgtcaccggc
     3601 gtcgcgcgcg acgaccttcc cgatgagggt gcgtggctgc acgccgagac cgtgcgccag
     3661 atccacgcgc agaaccccgg caccggcgtc gagattctcg cgaccgactt ctcgggaaac
     3721 cccgacctgc tgggcgaggt gttctcgtcg cgccccgaag tcttcgccca caacgtcgag
     3781 accgtgccgc gcatcttcaa gcgcatccgc ccggcgttcc gctacgagcg ttcacttgac
     3841 gtgctcaccc aggcccgggc ggccggcctc atcaccaagt ccaacctcat cctcggcatg
     3901 ggcgaggagc ccgccgaggt gctgcaggca ctgcaggacc tgcacgatgc cggcaccgac
     3961 atcatcacga tcacgcagta ccttcgcccg accccgcgcc acctgccggt cgcccgctgg
     4021 gtcaagccgc aggagttcgt cgagttctcg gaggccgccg aggagatcgg attcctcgga
     4081 gtgctcgcgg ggccgctcgt gcgctcgagc taccgtgctg ggcgcctgtg ggcgcgctcg
     4141 atggtggcga agggccgcga catcccggag cacctgactc acctcgcaga cgcgaacctc
     4201 ggcttcgcgc aagcggtctg agccgagcgc tgagccgcgt cgccgaaccc gtatcctaga
     4261 gacatggcac gaaccaccac gacggcgccg aaggctccca aggagcccgg tcgactgaag
     4321 cagatgtacc aggtcttcca gatgacccgg cgctacgaca agctcgcgat ctggtacctg
     4381 ctgctggcct tcctgctgcc ggtgctcgcg ggaatcatcc tcgcgatcgc tctgggtggc
     4441 gacagcattc tcgccgtcgt cctgtacgtg attgccggcg tgctcggtgg cgtactgctg
     4501 ttcctcatcg tgctcggccg tcgcgccgag aaggccgcgt actcgcagat cgagggccag
     4561 ccgggcgcag tgggtgcggt gctgaagagt tcgcttcgtc gcggctggac ggcgagcgag
     4621 atacccgtct cggtcagccc caagactcag gatgccgtgt accgcgccgt cgggcgtggc
     4681 ggtgtcgccc tcatcggcga gggcccccgc agccgcacgc agcgcatgct cgaggatgag
     4741 cgcaagagca tcgtgcgcat cctgccgaac gtgccggtgt cgttcatcta cgtgggaccg
     4801 gacgccgagt cggtgccgct gtacaagctg cccacgaaat tgcgccagtt gaagccggtg
     4861 ctgcgcaagc cggagatcct cgcggtttcc aaccgtctca actcgctggg caagaacggc
     4921 ctgccgattc ccaagggcat cgacccgatg cgagtgcgtt cgcagcgtcc gcgctaaggt
     4981 cagcgcctga cgagcaccgt gccgaccagt cgatcatgca tgccgcgctg gtcgagatcc
     5041 cagatgagtg cgggaatcac caggcacaac agcagtgtgc gcgcgaacgg tcgccagagt
     5101 cccaggtagc cgccggcgat cggaaccacg cgcatcccga gcagaagatg cccgggactc
     5161 gcattgatcg tgagcaggaa gagcacttgc acgccggcga agatcgccag ggttgcccac
     5221 gcctgatact ggaagaacgc cactgacacc agcacagcgg tcccccagtc gatcacgagg
     5281 gccgcgatcc ggcggcccaa ccggcccact gagcgcggtc cggaggcggg aagaccgagg
     5341 cgccgccccg gccagtcgga cgcggctgac gaggggtcgg gcatcccccg attctacgcg
     5401 tggggtgcgc ccgtaacatg cgcgaaacaa ttgcgtgatg ctggcgaaac cgcctaccca
     5461 taccctcatg agtggcggcg tctgctgccg atcagcacca agctcattgg agttcctcac
     5521 atgttcagtg attcttccga agtgctcaag ttcatcaagg acaccgacgt caagttcctt
     5581 gatattcgct tcaccgacct ccccggtgtt cagcagcatt tcaacattcc ggcgtccact
     5641 gtcgacgagg agttcttctc cgtcggtcag ctcttcgacg gctcgtcgat tcgcggtttc
     5701 gcgaacatcc acgagtcaga catgcagctg attccggatg tcacgaccgc ttacgttgac
     5761 ccgttccgca ccgagcgcac gctcatcatg gtcttcgaca tctacaaccc gcgcaacggc
     5821 gagatctacg ccaaggaccc gcgccaggtc gccaagaagg ccgagaagta cctcgcgtcg
     5881 accggcatcg cagacacggc attcttcgct cccgaggccg agttctacat cttcgacgat
     5941 gtgcgctact cggtgaccca gaaccagagc ttctactcgg tcgactccga ggagggcgcc
     6001 tggaacacgg gtcgcgtcga ggagggcggc aacctcgcca acaagacgcc tttcaagggt
     6061 ggctacttcc ccgtgagccc cgtcgacaag caggccgacc tgcgcgatga catcagcctc
     6121 aagctgatcg acgccggcct catcctcgag cgcgcccacc acgaggtcgg cacgggcgga
     6181 caggcggaga tcaactaccg cttcgacacg atggtgcatg cggccgacga catcctgaag
     6241 ttcaagtaca tcgtcaagaa caccgccctc gagtggggca aggtcgcgac cttcatgccg
     6301 aagccgctgt tcggcgacaa cggctcgggc atgcacaccc accagtcgct gtggaacgac
     6361 ggcaagccgc tgttctacga cgagaagggc tacggcggcc tgagcgacat cgcgcgctgg
     6421 tacatcggcg gcctgctcaa gcacgctccc gcggtgctcg cgttcaccaa cccgacgctc
     6481 aactcctacc gtcgcctcgt gcccggtttc gaggcgcccg tcaacctggt gtactcggcc
     6541 ggtaaccgct cggcgtcgat ccgcatcccg atcacgggta ccaacccgaa ggcgaagcgc
     6601 atcgagttcc gtgcaccgga tgcctcgggc aacccgtacc tcgcattcgc tgcccagctc
     6661 atggctggcc tcgatggcat caagaacaag atcgagccgc acgagcccgt ggacaaggac
     6721 ctgtacgagc tgccccccga ggaggcaaag ctcatccccc aggtgcccgg ttcgctggcc
     6781 gaggcgctcg acgcgctcga ggccgaccac gagttcctca ccgaaggcaa cgtcttcacg
     6841 caggacctca tcgacacctg gatcaactac aagcgcaccg cggagatcac tccgatggcg
     6901 cagcgtccgc acccgttcga gtacgagctg tacttctcgg tgtagtcgct acacagcaca
     6961 cggcggccgt cccttcgggg gcggccgctt tgttgtgcgg agggaggatt gcgctcccgc
     7021 gcggtcctaa tggacaaccg cggtcgttgt ggcgggcccc agcgtccata acacccgcgc
     7081 aagagggcgt gaggtcaccc cgtcgtggct gaccccagcc gcgccccgta gaaccgcttc
     7141 tcgaacacct ggcgcgcgag gcgcgtcgtg cgcaggtagt cctcctcgaa caccgtcgcc
     7201 gatcctggcg ggtactcgag caagcgcgcg acgccttcga gcgccatgcg gtcggtcggg
     7261 agcacatcgg aggtcttggc ggtccacagc gtcatgccgg accgggcccg ggaggcgagc
     7321 atccacgcat tgtgaagacg cgctgcgtcc gcggtcgtca cgaggccctc gcgctcggcg
     7381 gccgccagcg cgcccagagt cgacgtcgtg cgcagaccct ccaccttcgc accgtgctcg
     7441 agctgcagca gctgcacgaa ccactcgaca tcgctgagcg aaccacggcc gagcttcagg
     7501 tgtcgagcag gatcggcggc ctgcggcaga cgctccgcct ccacccgcgc cttgatgcgc
     7561 ttgatctcgc ggatgtcctg ctcggcgatc gtctcggggt agcgcacgtc gttgacgagt
     7621 tcctcccacg ccgcgaggac cgagcgatcg ccgaccacaa tgcgggcacg cagcagtgcc
     7681 tgggcctccc acgtgagcga ccagcgctcg tagtaggccc ggtagctctc cagtgagcgc
     7741 acgatggcgc cgttgcgccc ctccgggcgc agccctaggt cgaggtccag cggcacgcgc
     7801 atgtcctcgg tgaggcggct gatcgtgcgc acgatcgcct cggcccgctg ctgcgcctcc
     7861 tcatcggatg cccccgccgc ccgatagacg tacatcacgt cggcatccga gccgaagccg
     7921 agctccgctc ccccgtagcg tcccatggcg atgatgccga actcgatccc gtcgccgtag
     7981 tggtgagcga gggacagcgc gccgctgagg atgacggtgg tgatgtcgga gagccccttg
     8041 ccgaggtcct cgatggtcac gacgccgaga atcgcgccga gggccagccg cagtatctcg
     8101 cggcggcggg cggtgcgcag ggcgagagcc gcggcatcca cgtcaccctc gtggcgggcg
     8161 acggcggcga ccgactcctc aagcagtacc gcgtacgggc gcgggcgcag ctcgtcgtcg
     8221 ttctcgagcc acgcggcggc ctcggggatg cgctcgaaga tggcacccac gaacttggag
     8281 cccgacagca cctgcgtcaa ccgctgggcg gcggcgctgg agtcgcgcag catgcgcagg
     8341 aaccagtacg cctcgccgag gtcctcactg agcctgcgga acgcgagcag tccgtagtcg
     8401 gggtctgcgc catcggcgaa ccactgcagc atcacgggca ggaggttgcg ctggatcgtc
     8461 gccctgcgcg acacgccccc cgtgagcgcc gcgatgtggc tgagagcacc acgcgggtcg
     8521 cggaatccga tggccgccag tcgcgcctct gcctgttcgc tcgacagcgt caggccgtcc
     8581 tccggaaggg cggcgaccgc tgacagcagc gggcggtaga acagacgctc gtgcaagctg
     8641 cgtacggcct gcttggtgcg ctgccactgc tcgacgaggt cgtcggcgtt ggcggcgatt
     8701 cccgacgccc gcgcgaggat tcgcagggca tccggatcgg tgggcatgag gtgcgtgcgc
     8761 cgcagcctgc tgagctgaag acgatgctcg agcaggcgca ggaaccgata gtcaccggag
     8821 aactcggcgg cctccacccg accgatgtag ccgtgctcgg cgagggcgat cagtgcgggc
     8881 agcgtggcga tctggcgcac gttggggtcg ctctgtccgt gcaccagctg cagcagctgg
     8941 atcgtgaact cgacgtcgcg caggccgccg gggccgagct tgagctgcac gtccagctcg
     9001 tcggcgggga tattgtcggt gacccgctcg cgcatccgct gcaccgagtc gacgaagttc
     9061 tcccgcgacg cgctcgacca caccttcggc gacaccgcct ccacgtactg ccgaccgagt
     9121 tcgagatccc cagcgagcgg gcgtgccttc agcagcgcct ggaactccca actcttggcc
     9181 caccgctcgt agtacgcgac gtgcgactcg agggtgcgca cgagcgcccc gtccttgccc
     9241 tccggtcgca ggttcgcgtc gacctcccac aggccgggct cggtggccag atcgtggatg
     9301 ccgcgcatcg tctgcatcgc cagttgcgtc gcgatcgtca cggccctgtc gtccgcgaga
     9361 gtggactcac cgtcgagccc acccgcctcg cccacgaaga tgacgtcaac gtctgacacg
     9421 tagttgagtt cgcgcgctcc ggccttgccc attccgatga tggccagccg ggtcgcggcg
     9481 acctcctcgg cgggaaactt cacatcgcgg cgcgccacgg cgagggaggc gtcgagggcg
     9541 gcaccggaca gatcggccag agccgcggca acgatcgaga cggcggcgat cgggtcggcg
     9601 tgctccaggt cccacgcagc gagctgcacg aggtgcctgc ggtaccgcac ccgcagggca
     9661 acccacgcgg cgtcaccctt caggccgtcg actgcggcga ggaggtcggc tccgtactcg
     9721 gtggggggag ccgtgatcgg cgtcgccagt gcctgcaact cgcgcgggtg gcgcgcgagg
     9781 aagtcaccca ggccggagga ggccccgagc acacggatga ggcgggccgc ggcatccggg
     9841 tcggcgatca gggccgcaag ctcgaccggt gcgctctcgc gcagccccag aaggtagcgc
     9901 agcgcctgat ctgggtctgc ggcccgcgcg aagtgcgcaa ccagctcggg cgccccgagc
     9961 aggtccaggc gccgcgacgt ctcgtcgagc tcggtgaagc cgagtcgagc gagttcggtc
    10021 agcgtcgtcg gtggccggga catggcgggt gaatccacga gctagagaat ctcgaggttg
    10081 ctctccagct cgtacggggt gacctgcgca cggtactggc tccactcgcg gcgcttgttg
    10141 agcagcacgt agctgaagac ctgctccccc agcgtctccg cgacgagctc ggagtcctcc
    10201 atgagcgaca ctgcgtggtc aagactcgcg ggaagctggt tgtaccccag cgcgcgacgc
    10261 tcggcgtcgg agagctccca gacgttgtcc tcggcctcgg gaggcagctc gtagccctcc
    10321 tcgatgccct tgaggccggc cgcgagcaga agcgagaacg tcaggtaggg gttggccgcg
    10381 gagtcgatcg ctcggtactc aacgcgcgca ctctgaccct tgttgggctt gtacagcggt
    10441 acccgcacga gggccgaccg gttgttgtgg ccccaggtca cgaagctcgg tgcctcgtcc
    10501 ccaccccaga ggc
//
LOCUS       DAOSOU010000054        10426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6392_length_10426_cov_5.753447, whole genome shotgun sequence.
ACCESSION   DAOSOU010000054 DAOSOU010000000
VERSION     DAOSOU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_10426_cov_5.753447"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..399
                     /locus_tag="PLW15_04575"
     CDS             73..399
                     /locus_tag="PLW15_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33709.1"
                     /translation="MFGYACTYWTLTVDRHGVLLRGALTWPVIRIPLDQIESARVIDV
                     DPLSDFGGWGLRWAPRTTGIIVRRGEALEVARTGRRTLVVTLDRAAPGAELLNALVLR
                     QRGGVR"
     gene            625..3969
                     /gene="ileS"
                     /locus_tag="PLW15_04580"
     CDS             625..3969
                     /gene="ileS"
                     /locus_tag="PLW15_04580"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT33710.1"
                     /translation="MTYPRHTPDESVPPSPTFPSIEEQVLAYWREDDTFRASVDQREG
                     APEWVFYDGPPFANGLPHYGHLLTGYAKDLFPRFQTMRGKQVHRRFGWDTHGLPAELE
                     AERQLGITDKSQIEEMGIAAFNQAARDSVLKYTKEWEEYVTRQARWVDFENDYKTLDV
                     TFMESVIWAFKRLYDKGLAYEGYRVLPYCWRDQTPLSNHELRMDDDVYKMRQDQSVTV
                     TFPLDGPKAESLGLTAVKALAWTTTPWTLPTNLALAVGPDIEYAVVPSGPLGAGISTG
                     SITDAEGVAEFLLAADTVGAYAKDLGYATPDEALEAITRRLPGRDLEGVRYARLWDYY
                     ADTDEWGTGNAWQILVADYVATGEGTGIVHQAPAYGEDDQIACAAAGIPVILSVDEGG
                     KFLPIIEDVAGLQVFDANKPLTQKLRADGRLLRVASYEHSYPHCWRCRNPLIYKAVSS
                     WFVRVPEFRDRMEQLNQEINWVPENVKDGQFGKWIAGARDWSISRNRYWGSPIPVWKS
                     DNPEYPRVDAYGSLAELEADFGRLPLNAAGEPDLHRPYIDDLTRPNPDDPTGKSTMRR
                     IPDVLDVWFDSGSMPFAQVHYPFENEDWFATHNPADFIVEYIGQTRGWFYLLHVLSTA
                     LFDRPAFKNVISHGIVLGSDGQKMSKSLRNYPDVNEVFDRDGADAMRWFLMSSSVIRG
                     GNLVVTEEGIRGGVREFLLPLWSTYYFFTLYSNSRSGGLSSAERVSKPRENWRTDSTN
                     VLDRYLLAKTGQLIRDVTADLEALDSPLAAAKLRDFADVLTNWYVRRSRDRFWAGDDT
                     DAFDTLYTVLETVTRVAAPLIPLVGEEIWRGLTGGRSVHLEDWPDAASFPVDDTLVSA
                     MDRVREIASAGLALRKARGLRVRLPLARLTIVSNETAGLAPFGDILSEELNVKAVEFE
                     TLDTGSLDQHGIARRLTVNARALGPRLGKQVQQVIQAAKAGDWTVTDDVVTVGGTRLE
                     PGEFDLELEAADVASAIAFLSGDGFVILDTATTPELEAEGLARDVIRAIQDTRKAAGL
                     EVSDRIELAVTGSDAGDIDALTLFAETIAGETLAVAWVFELSADPALTATLGSAAGQR
                     AELAAGQYSNRGSLVIDVVKSGAVSV"
     gene            4085..5461
                     /locus_tag="PLW15_04585"
     CDS             4085..5461
                     /locus_tag="PLW15_04585"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT33711.1"
                     /translation="MDDDSEWDLEDSEYQDDADRVYEELLARVGEAAPQPRLEPTRRV
                     TELLGDPQRSYPIIHVTGTNGKTSTSRIAESILRAYGLRTGLMTSPHLVRVNERIVID
                     GQPISNRALAANWADIRPYIDMVDAELIANSEETLTYFEALTVLAFAAFADAPIDVAV
                     IEVGMGGEWDSTNVGDGQVAVFTPIALDHTQRLGSTVAEIARTKAGIIKPVASVVSAV
                     QSSDALAELERAVELTESSLEVEGDGFALESSAVAVGGQVITVRGLAETYSDLFLPMF
                     GDHQGHNAALAIAAVESFLGAGTQPLVGDVLAEGLATATSPGRLQVVGVDPTVIVDAA
                     HNPHGAAALAAALSTYFTFDEIVVVLGVLQDKDAAGIVAALAPVAARFNVTQSQSERA
                     VPHDQLAELVVEHAGQDSTYQFETFEQAVADARAWAAESDRRAVLVTGSITLVGEAIA
                     LAGSERWK"
     gene            5467..5880
                     /locus_tag="PLW15_04590"
     CDS             5467..5880
                     /locus_tag="PLW15_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HOT33712.1"
                     /translation="MSSPQGVSKPSKPPRRERTASESLLSIALALEAVLVFFVALTAF
                     GLKALEPLPAFIGGAALIVVLVLAARLVRYPWGVWLGWVLQVVLLATGILLPIMYVVA
                     AAFIGIWVYCFVKGRQLDSAKAAYLAANPQQPKES"
     gene            5881..6297
                     /gene="ndk"
                     /locus_tag="PLW15_04595"
     CDS             5881..6297
                     /gene="ndk"
                     /locus_tag="PLW15_04595"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT33713.1"
                     /translation="MSVEETLVLVKPDGVARNLTGEILRRIEAKGYQLVDIKLVQADL
                     DLLAAHYEEHQGKPFYEPLVEFMQSGPVVAIRVAGNRVIEGFRSLAGTTDPTTAAPGT
                     IRGDLGRDWGLKVQQNLVHGSDSPESAARELALWFG"
     gene            complement(6294..6893)
                     /locus_tag="PLW15_04600"
     CDS             complement(6294..6893)
                     /locus_tag="PLW15_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOT33714.1"
                     /translation="MTDAAAPRRSLSLAIVLIVTGAVGWYAALALTLDKLAVLANPEA
                     DLDCNFSVIVQCGKNLGSWQGAILGFPNPIIGLGGFVAPIAVGVALLAGATFARWFWI
                     AFNVGVAGAFAFCLWLAYQSIFNLGTLCPWCMLVWSMVIPMFWTLTLFNAREGRFGSG
                     LVRLGDRLYGWVPVITLAGYLIIAIVAQLRLDLLSMLAV"
     gene            7251..9698
                     /locus_tag="PLW15_04605"
     CDS             7251..9698
                     /locus_tag="PLW15_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT33715.1"
                     /translation="MVNENIEDSPKKKSRLFGRKAADAAAPATPPLEPEASEPSASDQ
                     AALAKAPARKRTAPKSAADHVNTAASDALVAEVATADVVAPAPARSRSKKAAPADGPA
                     EASTDSPADAAVDAPAKKSPPRPTTSLLFQAPDLPPLPPMPRSGAPLPELDEPGTVRR
                     RSRRRPGEDLRPGDEAPNTVVRVRQPRPEPVLSNEPTRVKGSTRLEAKKQRRRDGRDA
                     GRRRPVVTESEFLARRESVDRVMVVRSKFDRIQIAVLEDGVLVEHYVAKAQDASLIGN
                     VYLGRVQNVLPSMEAAFVDIGRGRNAVLYSGEVDWDAAALDSDGKTQARRIELALKPG
                     DRVLVQVTKDPVGHKGARLTSQVSLPGRYLVYVPNGSMSGISRKLPDTERARLKKILK
                     EVLPENVGVIVRTAAEGATEEQLTLDVNRLTNQWAEISRLVETQQAPALLHSEPDLLI
                     KIVRDVFNEDFGKLVIEGSDARQTIEGYLRSVAPDLLDRIQTYEGSADSFDEYRVSEQ
                     IEKALDRKVWLPSGGSLVIDRTEAMTVVDVNTGKFVGSGGNLEETVTKNNLEAAEEIV
                     RQLRLRDIGGIIVVDFIDMVLESNRDLVLRRLVECLSRDRTKHQVAEVTSLGLVQMTR
                     KKLGLGLLETFSETCEVCAGRGIIVHHDPVTKHRQQTQTAQPEQSGGRRNRRGGGNAG
                     GGAAVASTVKNGVGSNGTHAITEDVRNALARVASVTVHTTEIAIIPGNAPAEPAEAST
                     PVVETPETAPVTDSILDIPVVKQPRQQRKVSTQDAEQILGSVLEALPEPKQPGQGRSR
                     VSRRAGSAGQVVSAPSE"
     gene            complement(9753..10016)
                     /locus_tag="PLW15_04610"
     CDS             complement(9753..10016)
                     /locus_tag="PLW15_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HOT33716.1"
                     /translation="MTVLIDQPIWPAHNTLWAHIVSDTSLEELHAFAEAAGIPRRGFD
                     LDHYDVPAHKWHELVALGAQPVGVREFVQRLEASGLRVAQRDR"
     gene            10039..>10426
                     /locus_tag="PLW15_04615"
     CDS             10039..>10426
                     /locus_tag="PLW15_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33717.1"
                     /translation="MEISVKRMLRTRGALVAAITALAVGLTLVAGHLLLTVIAMEPFD
                     RAGYVLLTSVLPFTIGYFLALWVVAPITEQLGIGHVITRAVLATGIGATLWFVVLGLT
                     GVVTAVSATSDVLGIAASPGLIGAQLG"
BASE COUNT         1763 a         3619 c         3426 g         1618 t
ORIGIN      
        1 tccctgattc gagaggcata cgaatgatca ccacgtcacg ccgtcgagag caagtgatct
       61 gcgctcctgc tcgtgttcgg ctacgcctgc acgtactgga cgttgaccgt cgaccggcac
      121 ggggtgcttc tgcgtggcgc tctcacctgg ccggtcatcc ggattccgct cgaccagatc
      181 gagtcggctc gggtgatcga cgtcgatccg ctgtcggact tcgggggatg ggggcttcgc
      241 tgggcgccac gcaccacggg catcatcgtt cgtcgcggtg aggccctgga ggtggcccga
      301 acgggtcggc gcaccctcgt cgtcacgctc gatcgcgcgg cgccgggagc cgaactgctc
      361 aatgccctgg tcctccgtca gcgcggaggc gtgcggtagg attgtgagta taggcgccga
      421 tccggccatc accggggagc cttcggaaga gccgctctcg ggcggggtag aaccgaacgg
      481 gtcagcccgt tatagctctt gagtggcccg gaaccggtgg tcgaacacgt cgagaccatc
      541 gcccgagcaa gcggggtggt accgcggcta gtgcctgtgt caggccgatg tcgtcctcgt
      601 gcagagtcca gcacaggagc cacgatgact tacccccgcc acacccccga cgagtcggtg
      661 ccgccgagcc ccacgttccc gtcgatcgag gagcaggtgc tcgcctactg gcgcgaggac
      721 gacaccttcc gggcgtcggt cgatcagcgt gagggcgcgc ccgagtgggt gttctacgac
      781 ggtccgccgt tcgcgaacgg tctgccgcac tacggtcacc tccttaccgg ctacgcgaag
      841 gacctgttcc cgcgcttcca gaccatgcgc ggcaagcagg tgcaccgtcg cttcggctgg
      901 gacacccacg ggctgcccgc cgagctcgag gcggagcgtc agctcggcat caccgacaag
      961 agccagatcg aggagatggg catcgcggcc ttcaaccagg ccgcccgcga ctcggtgctg
     1021 aagtacacca aggagtggga ggagtacgtc acccgtcagg cccgctgggt cgacttcgag
     1081 aacgactaca agacgctcga cgtgaccttc atggagagcg tcatctgggc gttcaagcgc
     1141 ctctacgaca agggcctcgc ctacgagggc taccgcgtgc tgccgtactg ctggcgcgac
     1201 cagacgccgc tgtcgaacca cgaactgcgc atggacgacg acgtctacaa gatgcgtcag
     1261 gaccagtcgg tcacggtcac cttcccgctc gatgggccca aggcggagag cctcggcctc
     1321 acggccgtca aggctctcgc ctggacgacc accccgtgga ccctgcccac caacctcgcg
     1381 ctcgccgtcg gccccgacat cgagtacgcc gtcgtgccga gcgggccgct gggcgccggg
     1441 atctcgacgg gctcgatcac cgatgccgag ggcgtcgcgg agttcctcct cgccgccgac
     1501 accgtcggcg cctacgcgaa ggacctcggc tacgccacgc ccgacgaggc gctcgaagcg
     1561 atcacgcgcc gcctcccggg tcgcgacctc gagggtgtgc gctacgcccg cctctgggac
     1621 tactacgccg acaccgacga gtggggcacg ggcaatgcct ggcagattct cgtcgccgac
     1681 tacgtggcca cgggggaggg cacgggcatc gtgcaccagg cgcccgccta cggtgaggac
     1741 gaccagatcg cgtgtgctgc cgcgggcatc cccgtgatcc tgtcggtcga tgagggcggc
     1801 aagttcctgc ccatcatcga ggatgtcgcg ggcctgcagg tcttcgacgc caacaagccg
     1861 ctcacccaga agctgcgcgc cgacggtcgc ctgctgcggg tggccagcta cgagcactcc
     1921 tacccgcact gctggcggtg ccgcaatccg ctcatctaca aggcggtgtc gagctggttc
     1981 gtgcgcgtac ccgagttccg cgaccgcatg gagcagctga accaggagat caactgggtt
     2041 ccggagaacg tcaaagacgg ccagttcggc aagtggatcg ccggcgcccg cgactggtcg
     2101 atctcccgca accgctactg gggcagcccc atcccggtat ggaagagcga caacccggag
     2161 tacccgcgcg tcgacgccta cggctcgctg gccgagctgg aggccgactt cggcaggctg
     2221 ccgctgaacg cggcaggcga gcccgacctg caccgcccct acatcgacga cctgacccgg
     2281 cccaaccccg atgaccccac cgggaagtcg acgatgcgcc gcatccccga cgtgctcgac
     2341 gtgtggttcg attccggctc catgccgttc gcgcaggtgc actacccgtt cgagaacgag
     2401 gactggttcg ccacccacaa ccccgccgac ttcatcgtcg agtacatcgg ccagacccgc
     2461 gggtggttct acctgctgca cgtgctgtcg accgcactct tcgatcgccc cgccttcaag
     2521 aacgtcatca gccacggcat tgtgctcggc agcgacggcc agaagatgtc caagtcgctg
     2581 cgcaattacc ctgacgtcaa cgaggtcttc gaccgcgacg gcgcggatgc gatgcgctgg
     2641 ttcctcatgt cgagctcggt catccggggc ggcaacctcg tggtgaccga ggagggcatc
     2701 cgcgggggcg tgcgcgagtt cctgctgccg ctgtggagca cgtactactt cttcacgctg
     2761 tattcgaaca gtcggtcagg cgggttgagt agcgcggagc gcgtatcgaa accgcgcgaa
     2821 aactggcgca ccgactccac caacgtgctc gaccgctacc tgctggccaa gaccggccag
     2881 ctgatccggg atgtcacggc cgacctcgag gcgctcgaca gccctctcgc cgcagccaag
     2941 ctgcgcgact tcgccgacgt gctcaccaac tggtacgtgc gtcgcagccg cgaccgcttc
     3001 tgggcgggcg acgacaccga cgcgttcgac accctctaca cggtgctgga aaccgtgacc
     3061 cgggttgccg cgccgctcat cccgctcgtc ggcgaggaga tctggcgtgg gctcacgggc
     3121 gggcgcagcg tgcacctgga ggactggccg gacgcggcat ccttccccgt cgacgacacc
     3181 ctcgtctcgg ccatggaccg cgtgcgcgag atcgcgtcgg ccggactggc tctgcgcaag
     3241 gctcgcggtc tgcgcgtgcg cctgcccctc gcgcgactga ccatcgtctc gaacgagaca
     3301 gccggtctcg cgccgttcgg cgacatcctg agcgaggagc tcaacgtcaa ggcagtcgag
     3361 ttcgagactc tcgataccgg aagcctcgat cagcacggca tcgctcgccg tctcaccgtc
     3421 aacgcgcgcg ccctcggccc tcgcctgggc aagcaggtgc agcaggtcat tcaggcggcc
     3481 aaggccggcg actggacggt gactgatgac gtcgtcacgg tcgggggcac caggctcgag
     3541 ccgggggagt tcgacctcga actcgaggcg gccgacgtgg cgagcgccat tgcgttcctc
     3601 agcggcgacg gtttcgtcat cctcgatacc gcgacgaccc ccgaactcga ggcggaggga
     3661 ctcgcgcgcg acgtgatccg cgcgatccag gacacgcgca aggccgcggg actcgaggtc
     3721 agcgaccgca tcgagctcgc cgtcaccgga tccgacgctg gcgatatcga cgcgctcacc
     3781 ctgttcgccg agaccatcgc cggcgagacg ctcgccgtcg cgtgggtctt cgagctctcg
     3841 gccgaccccg cgctcaccgc cacgctcggt tcggccgccg gccagcgagc cgaactcgcg
     3901 gccgggcagt actcgaaccg tggttcactg gtaatcgatg ttgtgaagtc gggagcggtg
     3961 agtgtctgag tcaaaccagt ccgatcggga tgatgagttc gcggatgacg ccgacctcgg
     4021 cgacgacgac tcgaacccgc tcgccgatct cagcccgttt gcctcgggtg acgagggcac
     4081 gaacgtggac gacgactccg agtgggacct cgaggactcg gagtaccagg acgacgccga
     4141 ccgcgtctat gaggagcttc tggcccgcgt gggcgaggcc gcaccacagc cgcggctgga
     4201 gccgactcgc cgcgtgaccg aactgctggg cgacccgcag cgcagttacc cgatcattca
     4261 cgtgaccggc accaacggca agacgtcgac gagccgaatt gccgagagca ttctgcgcgc
     4321 ctacgggctg cgcaccgggc tcatgaccag tccccacctg gtgcgcgtca acgaacgcat
     4381 cgtgatcgat ggccaaccga tctccaaccg ggcgctcgcg gccaactggg cggacatccg
     4441 cccgtatatc gacatggtcg acgccgagct gatcgccaac tccgaggaga ccctcaccta
     4501 cttcgaggcc ctcaccgtgc tggccttcgc cgccttcgct gacgcgccga tcgatgtcgc
     4561 cgtcatcgag gtgggcatgg gcggcgagtg ggattcgacg aacgtgggcg acgggcaggt
     4621 tgccgtcttc accccgatcg ccctcgatca cacccagcgg ctcgggtcta cggtggccga
     4681 gatcgctcgc accaaggcgg ggatcatcaa gcccgtggca tccgtcgtct cggcggtgca
     4741 gtcctctgac gccctcgccg aactggagcg cgcggtcgag ctcaccgagt cgagcctcga
     4801 ggtggagggc gacgggttcg cgctcgagtc ctcggccgtc gcggtcggtg ggcaggtcat
     4861 cacggtccgc ggtctcgccg agacctacag cgacctgttc ctgcctatgt tcggcgatca
     4921 ccagggtcac aacgcggcgc tcgcgatcgc ggccgtcgag tcgttcctcg gggcgggcac
     4981 ccagcctctc gtgggcgatg tgctcgccga gggtcttgcg accgcgacgt cacccggacg
     5041 cctccaggtc gtcggcgtcg accccacggt gatcgtggat gccgcgcaca atccccacgg
     5101 cgccgcagcc ctcgccgcgg ctcttagcac ctacttcacg ttcgacgaga tcgtcgtcgt
     5161 gctgggcgtt ctgcaggaca aggatgccgc gggaatcgtc gcggcgctcg cccccgtggc
     5221 cgcccgcttc aacgtcaccc agtcgcagtc cgagcgcgct gtgccgcacg accagctggc
     5281 cgaactcgtt gtcgagcacg cgggtcagga ctcgacctac caattcgaga ccttcgagca
     5341 ggcggtggcg gatgcccggg cctgggccgc ggagagcgat cgccgcgcgg tgctcgtcac
     5401 cgggtcgatc acccttgtgg gagaagccat cgcgctcgcg ggttcggagc ggtggaagtg
     5461 agcgggttga gtagcccgca gggcgtatcg aaaccgtcga aacctccccg tcgcgagcgc
     5521 accgcgagcg agtccctcct ctcgatcgcc ctcgctctcg aggccgttct cgtcttcttc
     5581 gtcgcgctca ccgcgttcgg gttgaaggct ctcgagccgc tgccggcgtt catcggcggg
     5641 gccgccctga tcgtggtgct cgtgctcgcc gcgcgactgg tgcgctaccc gtggggcgtg
     5701 tggctgggat gggtgctgca ggtggtgctt ctagccaccg gcatccttct gcccatcatg
     5761 tacgtcgtcg ccgccgcctt catcggcatc tgggtctact gcttcgtcaa gggccgccaa
     5821 ctcgatagtg ccaaggccgc gtacctcgcg gcgaatcccc agcaacccaa ggagtcgtaa
     5881 gtgtcagtcg aagaaaccct cgttctcgtc aagccggatg gcgtcgctcg caacctcacc
     5941 ggtgagatcc tgcgccgcat cgaggcgaag ggctaccagc tcgtcgacat caagctggtg
     6001 caggccgacc tcgacctgct cgccgcccac tacgaggagc accagggcaa gccgttctac
     6061 gagcccctcg tcgagttcat gcagagcggt cccgttgtag ccatccgcgt cgccggcaat
     6121 cgggtcatcg agggcttccg ttcgctggca ggcacgaccg acccgacgac ggcagcgccg
     6181 ggaaccatcc gtggcgacct gggccgtgac tggggtctca aggtgcagca gaacctggtg
     6241 cacggcagcg actcacccga gtcggccgcg cgtgaactcg cactctggtt cggctagacg
     6301 gcgagcatcg acagcaggtc gaggcgcagc tgcgccacga tcgcgatgat gaggtagccg
     6361 gcgagggtga tcacggggac ccatccgtag agccggtcgc cgagccgcac gagccctgag
     6421 ccgaagcgac cctctcgagc attgaagagg gtgagggtcc agaacatcgg gatcaccatc
     6481 gaccagacga gcatgcacca cgggcacagg gttcccaggt tgaagatcga ctggtaggcg
     6541 agccagaggc agaaggcgaa cgccccggcg acgccgacgt tgaaggcgat ccagaaccaa
     6601 cgggcgaagg tggccccggc gagcagtgcc actccgacgg cgatcggagc gacgaatccg
     6661 ccgagcccga tgatggggtt ggggaagccg aggatcgcac cctgccacga cccgaggttc
     6721 ttgccgcact gcacgatgac gctgaaattg cagtcgaggt cggcctcggg attggcgagc
     6781 acggcgagct tgtcgagtgt cagcgcgagg gccgcatacc accccaccgc gcccgtgacg
     6841 atgagcacaa tggcgagcga cagggatctg cgcggggcgg cggcgtcagt catgttcgga
     6901 ttattgcacg ggccaaatca cgactaactg acaatgcgtg cgataatttg aggtagtcag
     6961 tcgagccgcg ctcgatctgg cgccaaaaca gctttaccgg ggcggttccc gggccaggaa
     7021 agcaacccca gaaccacggt tgcgcgagtc ggatagccag ttccgagtag tagtggcaga
     7081 ccaagtgaca acaagtgtgg tgagagcacg actgcaccgc gagcccagag cccggtgcag
     7141 gacctgaaga attagtagcg gtccctcgca atcagttgcg gcggatagcg cacgagagag
     7201 tacccggtgg gtggcgcggc cccctacccg ggaatgggag tacaccagca atggtgaatg
     7261 agaacatcga agatagcccg aagaagaagt cgcgactgtt tggacgcaag gcagcggatg
     7321 ccgcggcacc cgccaccccg ccgctcgagc ctgaggcatc cgaaccctcc gcgtccgacc
     7381 aggcagcgct ggcgaaggcc ccggcccgca agcgcacagc gccgaagagt gcggccgatc
     7441 atgtgaacac cgccgcaagc gatgcgttgg tcgcggaggt ggcgaccgcc gatgttgtcg
     7501 ctccggcgcc ggcccgcagc cgctccaaga aggcggcccc cgccgatggt ccggccgagg
     7561 catccactga ctcaccggcc gacgcagcgg tagacgcgcc ggccaagaag tcgccgccgc
     7621 gcccgaccac gtcgctgctc ttccaggcac ccgacctgcc tcctcttccg ccgatgccgc
     7681 gctcgggtgc accgctgccc gaactcgacg agcccggcac cgtgcgccgt cgttcgcgtc
     7741 gccgcccggg ggaggacctg cgccccggcg acgaggcgcc caacaccgtc gtgcgcgtgc
     7801 gccagccgcg tccggagccc gtgctcagca atgagcccac ccgcgtcaag ggctcaacgc
     7861 gcctcgaggc gaagaagcag cgtcgccgcg atggtcgcga cgccggccgt cgccgtcccg
     7921 tcgtcaccga gagcgagttc ctcgcccgcc gcgagtccgt cgaccgcgtc atggtggtgc
     7981 gcagcaagtt cgaccgcatc cagatcgccg tgctcgaaga tggcgtgctc gtcgagcact
     8041 acgtcgccaa ggcgcaggac gccagcctca tcggcaacgt ctacctcggt cgcgtgcaga
     8101 acgtgctgcc gtcgatggag gccgcattcg tcgacatcgg ccgcggtcgc aacgccgtgc
     8161 tctactccgg tgaggtcgac tgggatgccg cggctctcga ttccgacggc aagacccagg
     8221 cccgccgcat cgagctcgcg ctcaagccgg gcgaccgcgt gctcgtgcag gtcaccaagg
     8281 acccggtcgg acacaagggt gcccgcctca cctcgcaggt ctcgctgccc ggccgttacc
     8341 tcgtctacgt gcccaacggc tccatgtccg gcatcagccg caagctgccc gacaccgagc
     8401 gcgcccgtct gaagaagatc ctcaaggagg ttctgcccga gaacgtcggt gtcatcgtgc
     8461 gcaccgccgc cgagggtgca accgaggagc agctgacgct cgacgtcaac cgcctcacca
     8521 accagtgggc cgagatcagc cgtctcgtgg agacccagca ggcacccgcg ctcctgcact
     8581 ccgagcccga cctgctcatc aagatcgtgc gcgacgtctt caacgaggac ttcggcaagc
     8641 tcgtcattga aggctcggat gcgcgacaga cgatcgaggg ctacctccgt tccgtcgctc
     8701 ccgacctgct cgatcgcatc cagacctacg agggttcggc cgactcgttc gacgagtacc
     8761 gggtatccga gcagatcgag aaggcgctcg accgcaaggt ctggctgccc agtggtggtt
     8821 cgctcgtgat cgaccgcacc gaggccatga ccgtcgtcga cgtcaacacg ggcaagttcg
     8881 tcggctcggg cggcaacctc gaggagacgg tcaccaagaa caacctcgaa gccgccgagg
     8941 agatcgtgcg ccagctgcgt ctgcgcgaca tcggcggcat catcgtcgtc gacttcatcg
     9001 acatggtgct cgagtcgaat cgtgacctcg tgctgcgccg cctggtcgag tgcctgtcgc
     9061 gcgaccgcac caagcaccag gtcgccgagg tcacctcgct cggcctcgtg cagatgacgc
     9121 gcaagaagct gggcctcggc ctgctcgaga cgttcagcga gacctgcgag gtctgcgccg
     9181 gccgcggcat catcgtgcac cacgaccccg tcaccaagca ccgtcagcag actcagacgg
     9241 cgcagcccga gcagagcggc gggcgccgca atcgtcgggg cggcggcaac gccggcggtg
     9301 gggcggccgt ggcatccact gtcaagaacg gcgtgggcag caacggaact cacgcgatca
     9361 ccgaagacgt gcgcaacgcg ctcgcccggg tcgcatcggt gacggtgcac accaccgaga
     9421 tcgccatcat cccgggtaac gctcccgcgg agcccgccga ggccagcacc ccggtggttg
     9481 agactcccga aacggctccc gtgactgact cgattctcga catccccgtg gtgaagcagc
     9541 cgcgtcagca gcgcaaggtc tccactcagg acgcggagca gatcctcggg tcggtgctcg
     9601 aggcgctgcc ggagccgaag cagcccggac agggccgctc gcgcgtttcc cgacgtgcgg
     9661 gcagcgctgg gcaagtggtg agtgcgccct cggagtagcg ttcggccggt tgagtagccg
     9721 caccgcggcg tatcgaaacc gcgcgctccc actcaccgat cccgctgtgc cacccgcaaa
     9781 ccgctcgcct cgagacgctg cacgaattcg cgcacgccga ccggctgcgc gcccagcgcg
     9841 acgagctcgt gccacttgtg cgcgggaacg tcatagtggt ccaggtcgaa gccccgacgc
     9901 gggatgcccg ccgcctccgc gaacgcgtgc aactcctcca gcgacgtgtc actgacgatg
     9961 tgcgcccata gagtgttgtg tgcgggccag atcggctggt cgatgagcac ggtcatgatg
    10021 cgatcctagg caggcagcgt ggaaatctca gtgaagcgga tgctccgcac ccgaggcgcc
    10081 ctcgtggcgg ccatcacggc gctcgccgtg gggctcacgc tcgtcgccgg ccacctgctt
    10141 ctcaccgtga tcgccatgga gccgttcgat cgcgccggct acgtgctgct cacgagcgtt
    10201 ctgccgttca ccatcggcta tttcctggct ctctgggtgg tcgccccgat caccgagcaa
    10261 ctcggcatcg ggcacgtcat tactcgcgca gtcctcgcga caggcatcgg ggccacgctg
    10321 tggttcgtgg tgctggggct caccggcgtg gtgaccgctg tgtcggcgac ctccgatgtg
    10381 ctggggatcg cggcatcccc gggtctgatc ggtgcccagc tcggtt
//
LOCUS       DAOSOU010000055        10335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6460_length_10335_cov_5.347471, whole genome shotgun sequence.
ACCESSION   DAOSOU010000055 DAOSOU010000000
VERSION     DAOSOU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6460_length_10335_cov_5.347471"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLW15_04620"
     CDS             <1..509
                     /locus_tag="PLW15_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT33718.1"
                     /translation="DVVESGTSATLASALGLVDITPLAPSSDPARGIGRLGRLPVPMT
                     LGRLARTLGELLPATATGIRVAGDFDAPVTTVALCAGAGDSLLGHPAVLDADVYITSD
                     LRHHPASEARENARTAGGPALIDVSHWASEWLWLETAADELRAALPGVTVTVSDTRTD
                     PWDFTVTQ"
     gene            529..1269
                     /locus_tag="PLW15_04625"
     CDS             529..1269
                     /locus_tag="PLW15_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33719.1"
                     /translation="MALKASPDDQALLLDLQALDTKLAQLDHRARSLPELAALAALAT
                     EGDALRITKLEQQGLLENVQLELSRLESDVQVVETRIARDSERLQASSSVKDVAALEQ
                     ELSALRKRLDDLEEIELSVMEKLEEHQGRVDETSGRLAELLDQVAAIEATRDAALASI
                     GAERTTAAAGRRTIEAKVPADLLALYEKQRTRYGTGASLLRGGVSLASGVKLLEDELQ
                     KIRAAAPDDVLICPSSDAILVRTDESGL"
     gene            1269..1700
                     /locus_tag="PLW15_04630"
     CDS             1269..1700
                     /locus_tag="PLW15_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HOT33720.1"
                     /translation="MPRELVIEADGGSRGNPGQSGSGAVVIDPLTGEILAEVGLYGGI
                     ASNNVAEYKAMIAGVRRALAIDPDAELHIRMDSKLVVEQMSGRWKIKHPDMADLAAEA
                     RELLTGTPVRFEWIPRLQNSRADKLANLAMDMRADIGTRAE"
     gene            1715..2103
                     /gene="rnpB"
                     /locus_tag="PLW15_04635"
     ncRNA           1715..2103
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW15_04635"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2130..2717
                     /locus_tag="PLW15_04640"
     CDS             2130..2717
                     /locus_tag="PLW15_04640"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT33721.1"
                     /translation="MADENKRAGWYPDPDGGAGERWWNGASWSDSRRGAPAAAPVRAA
                     AASAGPASVAPHPVIPVPPPATVIYSAANPAPQALDPYAPGRTAVWTGAVLNAAPGRT
                     IDARVNRFAFYGFITGIISVFFNVLFVLAPLAIVFSIMGIARARQLKAQGAPATLMTM
                     ALIGLGTGAFSLLIGLIGLISVIAGVIGSMTFTAS"
     gene            complement(2714..3436)
                     /locus_tag="PLW15_04645"
     CDS             complement(2714..3436)
                     /locus_tag="PLW15_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT33722.1"
                     /translation="MRHTFETMGTVVSLEGVDDRTVEKVKSIFAAADSRFSLYRPESE
                     LARIASGEIAMMDSSVELRTAYADALAWSSLTSGAFSPNRPDGVVDLNGIVKAQAMQA
                     AGALIDAEGWREWSLNVGGDILIAGSTVQTTGIVDPFDRAKLLCAIELRPPRVAVATS
                     GSAERGDHIWLGGSTEPADYVQVTVVAGDIVTADVLATAIVAGGRSGLDEITERWDVD
                     VLAVDRTGGMVATPGIRESLAA"
     gene            complement(3436..3954)
                     /locus_tag="PLW15_04650"
     CDS             complement(3436..3954)
                     /locus_tag="PLW15_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT33723.1"
                     /translation="MRTRAVLGSIFASLGVLVIGWQAGNAAVMSATTATSTTTPTTTT
                     SGGNSGTSSSGTSNGSNTTTSTGTTTAAPVAPTTGATDGTYAGSTVNTRFGPVQVQVT
                     ISGGAITDVTALQLTNTDGRSVQISNYASPILAQEVLSSQSAQVSNVGGATYTSQAYL
                     QSLQSALDQAGF"
     gene            complement(3951..5372)
                     /locus_tag="PLW15_04655"
     CDS             complement(3951..5372)
                     /locus_tag="PLW15_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HOT33724.1"
                     /translation="MTTQAPAPTHQTTTRSRLPRINRRAQFARRARRADLLVTLLWAS
                     GAAAAALYLAYGGASQMTSVAGFITGLGILLGLVGTDFILVMLVLAARIPLLDRTIGH
                     DRAVATHRRLGKPALYLILAHGVLLLIGYGMSTGFNPIAEIGPMLALPDMPLAFIGTG
                     LLIAVVVTSLVAVRRKFSYEGWHLVHLLSYVAVAFALPHQLSVGGVLAEGTVQRVYWI
                     ALYVLAFGSILTFRFVEPIVASLRHRITVAGVSQIAPGVTSIHLKGSKLRSLGTAGGQ
                     FFIWRFWTPRTWWHSHPISLSAVPTDTTARITVRDLGAGSRRISTVPAGTRVSIEGPY
                     GLFTDAARTAPKMAIVAAGVGVTPVRALLEHATFAPGEATVLLRASSDDETYLWDEVR
                     AIAAAKGARLYTMVGRRSPVGPGWMPEEDARRGVTLRSIFPELGDSDLYLCGPTTWLD
                     LVEADARALGLPEHQIHAERFDW"
     gene            complement(5461..6777)
                     /locus_tag="PLW15_04660"
     CDS             complement(5461..6777)
                     /locus_tag="PLW15_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT33725.1"
                     /translation="MSIRARITIGSVLAAAVLLSVGLWLVREQVNSILTNTNAVLAEG
                     DLTSFETDILANPDEPVDDPGTGVLVFVRGPDGREQVDTLPRFLHGTIEHRDAADEQF
                     TIEHDGTTYVVVGRVVSTSSGDWGLWAARSEASSTLALASLDRLLAIGGLVLLACFGI
                     ASWLLASAALRPVRRMRERAETLGAESRDGRLPVGAARDELAALATTLNDFIQRVRAS
                     ASREKRMVSDAAHELRTPLAALRTQLELAHDDFGDADALAAQVTAAEASVGRLSSLAT
                     NLLELSRLESSESPRGSGSTSELVDELMGCIDRARMLGLAKQADVGFELRNTDRDKPY
                     GVDPQSFGRIAENLLANAVAAIPLRGSVTATLEQTPSDLILTVADTGPGMDEEFIPRA
                     FERFTRPDDSRTATTGGSGLGLALVRAIATAAGGTATLANSHPGLT"
     gene            complement(6789..7466)
                     /locus_tag="PLW15_04665"
     CDS             complement(6789..7466)
                     /locus_tag="PLW15_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33726.1"
                     /translation="MVRILVVEDDPTMGALVERGLREEGYEVTLVGNGMDALIAAAND
                     DFSAAAIDVMLPEMTGFEICRHLRQRGSTLPVLLLTARDAVEDRVFGLDSGADDYLTK
                     PFAFAEFSARIRALVRRDSATAKAVLRVGRLELDPMTVRAVIAGTPVALSTKEFALLS
                     LLVSRPGESLTRTEILQEVWGTTKHIDQTIVDQYVSYLRRKLEPTDSGVSIVTVRGVG
                     YQLREDA"
     gene            complement(7469..8197)
                     /locus_tag="PLW15_04670"
     CDS             complement(7469..8197)
                     /locus_tag="PLW15_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HOT33727.1"
                     /translation="MSIPDSPTGHTNEPHGDGIAHKLNWLRAGVLGANDGIVSVAAIV
                     VGVAGATTAVAPILTAGVAGLVGGAISMALGEYVSVSSQKDTQHALIAKERRELAEQP
                     DEELAELAGLFEKKGLTAATAQQVAVELTAHDALTAHLEIELGINERDVVSPWQAAGA
                     SALAFFVGALLPLLAILLPPENVRVPVTFAAVLIALALTGALSAWIGGSHWLRPTLRV
                     VIGGAIALVATFVIGTLLGTSGVV"
     gene            8354..8842
                     /locus_tag="PLW15_04675"
     CDS             8354..8842
                     /locus_tag="PLW15_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33728.1"
                     /translation="MTWEFNGLPIHILLIHFVVIVVPLAGLCTVLAAAWPAARRRLGI
                     VTPLIALAALIAVPITTEAGEVLEEQVAENAFSELHAQLGRDLLPWAIALFVVATVQW
                     TWFHYFSGTGRFANRITSKPARLAVTILLAVAVAVVVVGSVYTVFVIGESGAKAVWST
                     RV"
     gene            complement(8839..9513)
                     /locus_tag="PLW15_04680"
     CDS             complement(8839..9513)
                     /locus_tag="PLW15_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33729.1"
                     /translation="MKILIADDDPQMLRALRITLGARGYEVVTAADGRAALDAAIESH
                     PDVVVLDLGMPGLTGIEVIEAIRGWSQVPVLVVSGRSESWDKVEALDAGADDYVTKPF
                     SADELLARIRALSRRTPAPADEPTVSFGDVTVDLASHVVTRAGASVRLTPTEWRILEV
                     LLRHRDKLVTRETLLTEVWGPQYTTDTGYLRLYLSQLRKKLEPEPSAPRYLLTEAGMG
                     YRFTTA"
     gene            complement(9510..>10335)
                     /locus_tag="PLW15_04685"
     CDS             complement(9510..>10335)
                     /locus_tag="PLW15_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT33730.1"
                     /translation="RGRDLPASDRRILGAFLSQIEAALDRRELSREAESMRPVAEADR
                     LRTALLAAVGHDLRRPLAAATAAITSLRSTELTLSAADQDELLDAAEQSLDSLAGLVT
                     KLLDASRLQAGVLGVSLASVSVDDVVGGALDELGLAPGDVRLELERGIRPVIADAVLL
                     QRAVVNLLANALRFSPADAAPVVATSEFGDRVQLRVIDSGPGIPAERRDDVFVAFQRL
                     GDTDNTTGVGLGLALSKGFIEAMGGTLEAEDTPGGGLTMVADLPAVPNLLANAGEQ"
BASE COUNT         1687 a         3450 c         3592 g         1606 t
ORIGIN      
        1 ccgatgtcgt cgagagcgga acatcggcga ctctcgcctc cgccctcggc cttgtcgaca
       61 tcacgccgct cgcgcccagc tcggaccccg cccgcggcat tggccggctc ggacgactgc
      121 cagtgcccat gacgctgggg cgcctggcgc gcaccctcgg cgagctgctg ccggccaccg
      181 cgacgggcat ccgggtggct ggtgattttg atgcccccgt gaccacagtg gcgctgtgcg
      241 caggcgcagg cgactcactt ctgggccacc ccgccgttct cgacgccgat gtgtacatca
      301 cgagcgacct gcgtcaccac ccggcatcgg aggcacgcga gaacgcccgt accgctggcg
      361 gcccggcgct catcgacgtg tcgcactggg ccagcgagtg gttgtggctc gagaccgcgg
      421 ccgacgagct gcgggccgcg ctgccgggcg tgactgtcac cgtcagcgac accagaactg
      481 atccgtggga cttcacggtc acacagtagg actccaacag aggaaacgat ggcactgaaa
      541 gcatcacccg acgaccaggc actcctgctg gaccttcagg cgctcgatac caagctcgcc
      601 cagcttgatc accgcgcacg gagcctgccc gagctggctg cgctcgccgc cctcgcgacc
      661 gagggcgatg cgctgcgtat caccaaactg gagcagcagg gtctcctcga gaacgtgcag
      721 ctcgagttgt cgcggctcga gagcgacgtg caggtggtcg agacgaggat cgcgcgcgac
      781 agcgagcgcc ttcaggcaag ctcgtcggtc aaggatgtcg ccgccctcga gcaggagctc
      841 agcgcgctgc gcaagcgctt ggacgatctc gaggagatcg agctgagcgt catggagaag
      901 ctcgaagagc accagggccg ggtcgatgag accagtggac gcctcgcgga gctgctcgat
      961 caggtcgccg cgatcgaagc gaccagggat gccgcgctcg catccatcgg cgccgagcgc
     1021 accaccgcgg ccgcgggccg tcgaaccatc gaggccaagg taccggcgga cctcctcgcc
     1081 ctctacgaga agcagcgaac tcgatacgga accggtgcct cactgctgcg cggcggcgtc
     1141 tcgctcgcct cgggggtgaa gctgctcgaa gacgagctgc agaagatccg cgccgcggcg
     1201 cccgacgacg tgctgatctg cccgtcgagc gacgcgattc ttgtgcgcac tgacgagtcc
     1261 ggcctctgat gccacgcgaa ctcgtcatcg aggccgacgg cggctcccgc ggcaatcccg
     1321 gacagtcggg cagcggtgcg gttgtcatcg atccgctgac cggtgagatc ctcgccgagg
     1381 tgggactcta cggcgggatc gcgagcaaca acgttgccga gtacaaggcg atgatcgccg
     1441 gggtgcgccg cgccctcgcc atcgatcccg acgccgagct gcacattcgg atggactcca
     1501 agctcgtcgt cgagcagatg tcggggcggt ggaagatcaa gcaccccgac atggccgacc
     1561 tcgccgccga ggcacgggag cttctgacgg gcacgcccgt gcgcttcgaa tggattcccc
     1621 ggctgcagaa ttcgcgggcc gacaagctcg cgaacctcgc gatggacatg cgcgcggaca
     1681 tcggcacacg agccgagtag gcttgccact cgaatgggtc ggcaagacgg tcgcggctcg
     1741 ccttcgggtg ggtcgaggaa cgtccgggct ccgcagagca ggatggtggg taacacccac
     1801 ccggggcaac ccgcgagaac agtgcaacag aaagcagacc gccgccgggc agtcgtcgaa
     1861 agacgcgcgg cccggaggta agggtgaaac ggtggtgtaa gagaccacca gcggccgcgg
     1921 cgacgtggtc ggctaggtaa acctcgtccg gagcaaggtc agacagggaa cgttacaaag
     1981 gctgctcgcc cagttcccgg gtagaccgct agagggctgc ggcaacgtag tccccagata
     2041 gatggccgtc gatggtgctc tcgagcaccg ggacagaacc cggcgtatca gccggcccat
     2101 tcactccccg ccgtccctaa actgtctgca tggccgacga gaacaagcgg gcgggatggt
     2161 atccagaccc cgatggtggc gccggtgagc gctggtggaa cggcgcgagc tggagcgact
     2221 cgcgccgcgg ggcgcccgcc gctgcgcccg tgcgtgcggc cgcggcatcc gctggcccag
     2281 catcggtcgc cccgcacccg gtgatccccg tgccgccgcc cgccaccgtg atctactcgg
     2341 ccgcgaaccc ggctccgcag gcgctcgatc cgtacgcccc cggcagaaca gctgtgtgga
     2401 cgggcgccgt cctcaatgcc gcgccgggcc gcacgattga cgccagggtc aacaggttcg
     2461 cgttctacgg cttcatcacc ggcatcatct cggtgttctt caacgtgctg ttcgtgctgg
     2521 cgccgctcgc gatcgtgttc tcgatcatgg gtatcgcgag ggcgcgtcag ctgaaggccc
     2581 agggcgcgcc cgcgacgctg atgaccatgg ccctgatcgg attgggaacc ggcgccttct
     2641 cgcttctcat cggcctgatc ggtctgatca gcgtgatcgc gggcgtcatc ggctcgatga
     2701 ccttcaccgc cagctaggcg gccagcgact cccggatacc cggggtggca accatcccgc
     2761 cggtgcgatc cactgcgagc acatccacgt cccaccgctc ggtgatctcg tcgagaccgg
     2821 atcgtccgcc cgccacgatc gcggtcgcca gcacgtcggc cgtgacgatg tcgccagcga
     2881 cgaccgtgac ctgcacgtaa tcggctggct cagtagaccc gccaagccag atgtggtcgc
     2941 cgcgctcggc gctgcccgat gtcgcaacgg cgacgcgggg cgggcgcagt tcgatcgcgc
     3001 agagcagttt cgcgcggtcg aaggggtcga cgatccccgt ggtctgcacg gtcgatccgg
     3061 cgatcaggat gtcgccgccc acgttgaggc tccactcgcg ccacccctcg gcatcgatga
     3121 gcgcgccggc cgcctgcatg gcctgtgcct tcacgatgcc gttgaggtcg acgacgccgt
     3181 ccgggcggtt cgggctgaac gcgcccgagg tgagcgagct ccaggcgagg gcgtcggcgt
     3241 aggcggtgcg tagctcgacg ctcgagtcca tcatggcgat ctcacccgac gcgatccggg
     3301 ccagctccga ctcgggccgg tagaggctga atcgcgagtc cgcggcagcg aagatgctct
     3361 tgaccttctc gacggtgcgg tcgtcgacgc cctcgagcga cacgacagtg cccatcgtct
     3421 cgaaggtgtg acgcatcaga acccggcctg gtccagtgcg gactgaaggg actgcaggta
     3481 ggcctgcgag gtgtacgtcg cgccacccac gttcgacacc tgagccgact gcgacgagag
     3541 cacctcctgc gcgaggatcg gcgacgcgta gttgctgatc tgcacggagc ggccatcggt
     3601 gttggtcagc tgaagggcgg tgacgtcggt gatggcgccg ccggagatcg tgacctgcac
     3661 ctgcacgggc ccgaaacgcg tgttcacggt ggagcccgcg taggtaccgt cggtcgcgcc
     3721 ggttgtcggt gccaccgggg cggcggtcgt ggtgccggtg ctcgttgtcg tgttcgagcc
     3781 gttggaagtt cccgatgacg acgttccgga gtttccaccg ctggtcgtgg tggttggcgt
     3841 ggtcgtgctc gtggcggtcg ttgcgctcat gacggcggcg ttacccgcct gccatccgat
     3901 caccaacacc cccagcgaag cgaagatgct gcccagtact gcgcgtgttc tcaccagtcg
     3961 aacctttctg cgtggatctg gtgctcggga agtcccagag cgcgggcgtc cgcctccacc
     4021 aggtcgagcc aggtggtggg gccgcagaga tagagatcgg agtcgccgag ttcggggaag
     4081 atgcttcgga gagtgacgcc gcgacgggca tcctcttcgg gcatccagcc gggaccgacg
     4141 ggcgagcgcc gtccgaccat ggtgtacagc cgggcgccct ttgcggcggc aatggctcgc
     4201 acctcgtccc agaggtaggt ctcgtcgtcg ctgctcgccc tcagaagcac ggtcgcctcg
     4261 ccgggggcga aggtggcgtg ctcgagcagg gcgcgcacgg gggtgacgcc gaccccggcc
     4321 gcgacgatgg ccatcttcgg ggcggtgcgg gcggcatccg tgaacaggcc gtacgggcct
     4381 tcgatcgaca cccgagtgcc cgcaggaacc gtgctgatgc ggcggctgcc cgcgccgagg
     4441 tcgcgcaccg tgatccgcgc ggtggtgtcg gtgggaaccg ccgacagcga gatcgggtgc
     4501 gagtgccacc aggtgcgggg tgtccagaag cgccagatga agaactggcc gcccgccgtg
     4561 ccgagcgagc gaagcttgct gcccttcagg tggatggacg tgaccccggg cgcgatctga
     4621 ctgaccccgg cgaccgtgat tcggtggcgc aggctcgcga cgatcggctc gacgaagcgg
     4681 aaggtcagga tcgacccgaa ggcgagcacg tagagcgcga tccagtagac ccgctgcacg
     4741 gtgccctcgg cgaggacccc gccgacgctg agctggtgag ggagtgcgaa cgcgaccgca
     4801 acgtagctca gcaggtgcac caggtgccag ccctcgtagc tgaacttgcg gcgcaccgcg
     4861 acgagcgagg tgaccacaac ggctatgagc aggcccgtgc cgatgaacgc cagcggcatg
     4921 tcggggaggg cgagcatggg gccgatctcc gcgatcggat tgaagccggt gctcattccg
     4981 tagccgatga gcagaagcac cccgtgggcg agaatcagat acagggcggg cttgccgagc
     5041 ctgcggtggg tggcgaccgc tcggtcatga ccgatcgtgc ggtcgagcag cggaatgcgt
     5101 gcggcgagca ccagcatcac gaggatgaag tcggtaccca cgagtccgag cagaattccc
     5161 agtccggtga tgaagccggc gaccgacgtc atctggctcg cgccgccgta ggccagatac
     5221 agggcggccg cggccgcacc ggacgcccac agaagggtta ccagcagatc ggcgcgacgc
     5281 gcacgacgag cgaactgagc tcggcgattg atccgcggca gccgcgaacg agtcgtggtc
     5341 tggtgagtcg gagccggggc ctgggttgtc atgtctacac actgctcgcg tgagctatgc
     5401 ggatcctatg agcggcgggc ccccagcatg agagttctca cattgtcggc agggtcacgg
     5461 tcaggtcagg ccgggatgcg agttggccag ggtggcggtg ccgccggctg ccgtggcgat
     5521 cgcccgcacg agtgcaagtc cgaggccgct tccgccagta gtcgccgtgc gcgagtcgtc
     5581 aggtcgtgtg aagcgctcga acgctcgcgg aatgaactcc tcgtccattc ctgggcccgt
     5641 gtctgccacc gtcaggatca ggtcggaggg cgtctgctcc agtgtcgcgg tcaccgagcc
     5701 ccgcaacggg atcgcggcga cagcgttggc cagcaggttc tcggcgatgc gtccgaagga
     5761 ttgcgggtcg acgccgtacg gcttgtcgcg gtcggtgttg cgcaactcga aaccgacatc
     5821 ggcctgtttg gcgaggccca gcatccgggc gcgatcgatg cagcccatca gctcgtcgac
     5881 gagctcactg gtcgagcccg atcctcgcgg tgactcgctc gactcgaggc gggagagctc
     5941 gagcagattg gttgccagcg atgacagccg accgacggat gcctccgccg cagtcacctg
     6001 tgcggcgagc gcgtcggcgt ccccgaagtc gtcgtgcgca agttcgagct gcgtgcgcag
     6061 cgcggcgagc ggcgttcgca gctcgtgcgc cgcgtcggac accatgcgct tctcccggct
     6121 cgctgatgcc cgcacccgct ggatgaagtc gttgagggtc gtggcgaggg cggccagctc
     6181 gtcgcgcgcc gcgccgaccg gcagccggcc gtcgcgggac tctgcgccga gggtctccgc
     6241 tcgctcgcgc atgcggcgca ccggacgcag cgctgctgac gccagcagcc aggacgcgat
     6301 cccgaagcag gcgagaagta cgagcccgcc gatcgcgagc agtcgatcga gcgaggcgag
     6361 tgccagcgtg ctcgatgcct cgcttcgcgc cgcccacaga ccccagtcgc ccgaactggt
     6421 gctgacgacc cggccgacga cgacgtaggt cgtgccgtcg tgttcgatcg tgaactgttc
     6481 gtcggccgcg tcacggtgct cgatggtgcc gtgcaggaat cgcggcaggg tgtccacctg
     6541 ctcgcggccg tcggggcccc tcacgaacac gagaactccc gtgcccgggt catcgaccgg
     6601 ctcgtcgggg ttggcgagga tgtccgtctc gaacgaggtc agatcccctt cggcgagcac
     6661 ggcattcgtg ttcgtgagaa tgctgttgac ctgctcccgc accagccaca gcccgaccga
     6721 caggagtacg gcagcggcca ggacgcttcc gatcgtgatc cgggcgcgga tggacaatcg
     6781 cgagagcatc acgcgtcctc gcgcagctgg tagccgacac ctcgtacggt cacgatcgag
     6841 acgccggagt cggtgggctc cagcttgcgc ctcaggtagc tgacgtactg gtcgacgatg
     6901 gtctggtcga tgtgcttggt ggtgccccag acctcctgaa ggatctcggt gcgggtcagc
     6961 gactcgccag gtcgcgacac gagcaggctg agcagcgcga actccttcgt gctcagcgca
     7021 acgggcgtgc cggcgatcac cgctcgaacc gtcatgggat cgagttcgag ccgtcccacc
     7081 cgcagcacgg ccttggcggt ggccgagtct cgtcgcacca gcgcgcggat tcgcgcgctg
     7141 aactcggcga aggcgaacgg cttggtgagg tagtcgtcgg ccccggagtc cagaccgaac
     7201 actcggtcct ccacagcatc ccgagccgtg agcagaagaa ccggaagcgt gctcccccgt
     7261 tggcgcaggt ggcggcagat ctcgaatcct gtcatctcgg gcagcatcac atcgatcgcg
     7321 gcggcgctga agtcgtcgtt cgcggcagcg atcagcgcat ccatgccgtt gccgacgagg
     7381 gtgacctcgt agccctcctc gcgcaggccc cgttcgacga gggctcccat ggtcgggtca
     7441 tcctcgacga cgaggatccg gaccacggct acaccacgcc ggaggtaccg agaagggtgc
     7501 cgatgacgaa cgtcgcgacc agcgcgatgg caccaccgat gacgacgcgg agggtgggcc
     7561 gcagccagtg gctgccgccg atccacgcgc tcagcgcgcc ggtcagggcg agcgcgatga
     7621 ggacggccgc gaaagtcacg ggaacccgca cgttctccgg cggcagcagg atggcgagca
     7681 acggcagcag cgcgccgacg aagaatgcca gagcggaggc ccctgcggcc tgccacgggc
     7741 ttaccacatc tcgctcgttg atgccgagct cgatctccag gtgcgcggtg agtgcgtcgt
     7801 gcgcggtgag ctcaacggcg acctgctgag cggtcgcagc cgtgaggccc ttcttctcga
     7861 acagtccggc gagctcggcg agctcctcat caggctgctc ggcgagctcg cgacgctcct
     7921 tggcgatgag ggcgtgctgg gtgtccttct ggctcgatac cgacacgtac tcgccgagag
     7981 ccatcgagat ggcaccaccg accagcccgg cgacaccggc cgtgagaatc ggcgcgacag
     8041 cggtcgtcgc acccgcgacg ccgacaacga tcgccgcgac cgagacgatg ccgtcgttcg
     8101 caccgagcac cccggcacgc agccagttga gcttgtgggc tatcccatcg ccgtgcggtt
     8161 cgttggtgtg tccggtcggg gagtcaggaa tgctcatgcc gacagctaac acccagaccg
     8221 ggcatccatt ccagtcaggc aaggctaggc ttcgtgcgct gtggaagggg ctgctgcggg
     8281 gctcggagtg gatgcgcgca tccgcgggac tttcgtcccg cccgctgcag acgcgccgtg
     8341 ccaagctcgc gctatgacct gggaattcaa cggactaccg attcatattc tgctgatcca
     8401 cttcgtggtg atcgtggtgc cgctggcagg gctctgcacg gtgctggctg cggcctggcc
     8461 cgccgcgcgc cgtcgactcg gcatcgtcac gccgttgatc gctctcgctg ctctgatcgc
     8521 ggtgccgatc accaccgagg cgggggaagt gctcgaagaa caggtcgccg agaacgcatt
     8581 cagcgaactg cacgcccagc tggggcgtga cctgctgccg tgggcgatcg cactcttcgt
     8641 tgtcgccaca gtgcaatgga cctggttcca ctacttcagc ggcacgggcc gtttcgcgaa
     8701 ccgcatcacc tcgaagcctg cgcgcctcgc cgtgacgatc ctgctcgctg tggctgtcgc
     8761 ggttgtcgtc gtcggatccg tctacacggt attcgtcatc ggggagtccg gcgcgaaggc
     8821 ggtctggagc acgcgggtct aagcggtggt gaagcggtac cccatcccag cctcggtcag
     8881 aaggtaccgc ggtgcggagg gttcgggctc gagcttcttg cgcagctgcg agaggtagag
     8941 ccggaggtat ccggtgtcgg tcgtgtactg cggtccccag acctcggtca gcagggtctc
     9001 gcgcgtgacg agcttgtcgc ggtggcgaag cagcacctcg aggatgcgcc actcggtcgg
     9061 cgtaagccga acggatgcac cggcacgggt caccacgtgg gaggccaggt caacggtcac
     9121 atcgccgaac gacacggtgg gctcatccgc gggtgcggga gttcgacgcg acaatgcccg
     9181 gatgcgcgcg agcagctcat cggctgagaa cggcttggtc acgtagtcgt ctgccccggc
     9241 gtcgagcgcc tccaccttgt cccagctctc gctgcgaccg gacacgacga gcacgggcac
     9301 ctgactccag ccgcggatgg cctcgatcac ctcgatgccg gtgaggccgg gcatccccag
     9361 gtcgagaacg acgacgtcgg gatgcgactc gatcgcggcg tcgagagccg cgcgcccgtc
     9421 ggcggccgtg accacctcgt acccgcgcgc tccgagcgtg atccgcagtg ccctgagcat
     9481 ctggggatcg tcgtcggcga tcaggatctt cactgctcac ccgcgttcgc gagaagattg
     9541 ggcaccgcgg gcaggtcggc gaccatggtc agtccgcctc cgggggtgtc ctcggcctcg
     9601 agcgtgccgc ccatcgcctc gatgaagccc ttggacagtg ccagccccag gccgaccccc
     9661 gtggtgttgt cggtatcgcc caggcgctgg aatgccacga agacatcatc ccgccgttcc
     9721 gcgggaattc cgggacccga gtcgatgacc cgcagctgca cgcggtcacc gaactcgctg
     9781 gtcgcgacga caggggcggc gtcggccgga gagaagcgca gcgcgttggc cagcaggttg
     9841 acgacggcgc gctgcaggag cacggcgtcg gcgatcaccg gtcggatgcc gcgctccaac
     9901 tccaaccgca cgtcgccggg tgcgaggccc aactcatcga gcgcaccgcc caccacatcg
     9961 tcgacggaca ccgaagccag tgacaccccc agtacccccg cttgcaggcg actggcgtcg
    10021 agcagcttgg tcacgagacc cgcaagcgag tcgaggctct gctcggcggc atccagcagc
    10081 tcgtcctgat cggccgccga cagggtgagc tcggtcgagc gcagcgaggt gatcgctgcg
    10141 gtcgctgccg cgagggggcg ccgcaggtca tgcccgacgg cggccagcag cgcggtgcgc
    10201 aggcgatcgg cttctgcgac cggtctcatg ctctcggcct ctcggctgag ctcccgtcga
    10261 tcgagggccg cctcgatctg ggagaggaat gcgccgagta tgcgtcggtc cgatgccggc
    10321 aggtcacgcc cccga
//
LOCUS       DAOSOU010000056        10175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6592_length_10175_cov_6.528360, whole genome shotgun sequence.
ACCESSION   DAOSOU010000056 DAOSOU010000000
VERSION     DAOSOU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6592_length_10175_cov_6.528360"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLW15_04690"
     CDS             <1..227
                     /locus_tag="PLW15_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOT33731.1"
                     /translation="AINGRTYRIAGRIAMDQVVVDVGDTPVSVGDRAVLFGDPSTGAP
                     SADEWAAAAGTINYEIVTRIGPRVARRYRG"
     gene            224..703
                     /gene="tsaE"
                     /locus_tag="PLW15_04695"
     CDS             224..703
                     /gene="tsaE"
                     /locus_tag="PLW15_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT33732.1"
                     /translation="MRIETPEAMALLGARIAAELRAGDLVLLNGELGAGKTTLTRGIG
                     EALGVRGAVTSPTFVLARTHPRVEAGTVDDAPLVHVDAYRLGSAMELDDLDIDFPASI
                     VVVEWGADLTDGITDDWLQIDIVRPHGGDESAEVEPRDVTLTGHGERWGRWVDALGD"
     gene            687..1307
                     /gene="tsaB"
                     /locus_tag="PLW15_04700"
     CDS             687..1307
                     /gene="tsaB"
                     /locus_tag="PLW15_04700"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT33733.1"
                     /translation="MLLAIDTSAGTSVAIVDRDAGVIAEHSSPDTRGHAELIGTFIQA
                     CLDDWGGSVTTLSGVVSGMGPGPFTGLRVGIAAARAFAFGAGKIVVPVVSHDAVAFGA
                     EGPVLVVTDARRHELFWSTYSGSDASGLPVRTSGPNLAKAAELEHDVPGYDGYRLLDS
                     GLVSAASLGLIAELQYAHGRPFAADQPLYLRSPDVTLSAGPKRVTS"
     gene            1304..1789
                     /gene="rimI"
                     /locus_tag="PLW15_04705"
     CDS             1304..1789
                     /gene="rimI"
                     /locus_tag="PLW15_04705"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT33734.1"
                     /translation="MTAWQLRQATVDDLDAIMQLETALFESDAWSRSSMKAELADRHG
                     YYLVAFPVGQPEQVDGYAGLRAPRGEAQADIQTIGVAEHARRQGLGRVFMLRLIDEAR
                     ERGATEVFLEVRADNPGAQALYDSLGFERIAVRPKYYQPDGVDAIIMRLTIPAPQMGQ
                     A"
     gene            1786..2829
                     /gene="tsaD"
                     /locus_tag="PLW15_04710"
     CDS             1786..2829
                     /gene="tsaD"
                     /locus_tag="PLW15_04710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT33735.1"
                     /translation="MIAPLVLGIETSCDETGIGIVRGTTLLANVIASSMQEHARYGGV
                     VPEVAARAHLEALTPTLREALDRAGVALGDIDAVAVTSGPGLAGALMVGVGAAKALAL
                     ALDKPLYAVNHLVGHVGADVLDERPLDLPTIALLVSGGHTSLLLVRDLVSDVELLGET
                     IDDAAGEAFDKVARLLGLPYPGGPEIDRAAVGGDPTAIRFPRGLSLPKDMEKHRYDFS
                     FSGLKTAVARWVERERDAGHELPLADVAASFREAVVDVLLTKALAACRDLDVPRLLLG
                     GGVVANARVRELAAQRCAAAGVELRIPPLALCTDNGAMIAALGAQLVMAGRVPSGLGF
                     GADSTLPVTTIQA"
     gene            2926..3207
                     /locus_tag="PLW15_04715"
     CDS             2926..3207
                     /locus_tag="PLW15_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOT33736.1"
                     /translation="MTEPTPAPSYAPAAKWNVLSIVSLVTAIVGLGLVAVITGHISLS
                     QIKKTGEQGNILAIIGLILGYLEIVGVIIFGIVWIGIIAAAGTAGMYSN"
     gene            3289..3906
                     /locus_tag="PLW15_04720"
     CDS             3289..3906
                     /locus_tag="PLW15_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOT33737.1"
                     /translation="MADKPADNVPPVPVPPSSAVPPVEPAPKVRKTAAPKTTATAAAA
                     ATPAKAAAPKATPAPKATAAAKTPAAKTPAAPKAPAAAVVPPAPPVAVAPPAPPAPPA
                     AQTPPAYAPSAYTPAPAGPAQGLSITSMVLGIAGLFLGFLVSIAAVITGHMAQKRQPY
                     AKPFWLTGIITGYIGIALGLIVTIAFIAWIAFIASVAGAGGYYNY"
     gene            complement(3903..5078)
                     /locus_tag="PLW15_04725"
     CDS             complement(3903..5078)
                     /locus_tag="PLW15_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT33738.1"
                     /translation="MERDEVEQILSVEGLRLLDALPRYESTADVVRVVSDLRAAGHPA
                     GLVAAVLNQSKLRARAEAKFGPFAASMLFTEAGLEQATRLSVAAQHAGRFQRAGLNWI
                     ADLGCGVGADALAIAALELEVTAVERDEVTAAIAAYNLAPWSNARVEHADAESFDLAG
                     VDGVYLDPARRDGVKRLKNPADWSPSLDFAYGLAQRLPVGVKLGPGIDRDLIPNDAEA
                     QWVSVDHDVVEVGLWFGTLARQNVRRAALVIGQHGAAELTAESDSEDVPVGSLGQFLY
                     EPDGAVIRARLIGDLGRLIGGRMLDRTIAYLTTDEPAETPFAQGFRIVADFALDQRSL
                     KRELASRGIGTVEIKKRGVDIDPAAFRTALSPKGENSATLFLTRVGGKRRALLAERL"
     gene            5277..5573
                     /gene="groES"
                     /locus_tag="PLW15_04730"
     CDS             5277..5573
                     /gene="groES"
                     /locus_tag="PLW15_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT33739.1"
                     /translation="MSVSIKPLEDRIVIKQVEAETTTASGLVIPDTAKEKPQEGEVVA
                     VGPGRIDDNGNRIPLDIAVGDKVIYSKYGGTEVKYGGEDLLVLSARDVLAVVVR"
     gene            5654..6586
                     /gene="rarD"
                     /locus_tag="PLW15_04735"
     CDS             5654..6586
                     /gene="rarD"
                     /locus_tag="PLW15_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOT33740.1"
                     /translation="MPASVPSHERVSAPGIAFAIASYLLWGVLPLLFLALPPTGAPEI
                     VAWRIVLSLAFCVILLTVTGGWSRFMALVRDRVALGTLALAAVLVLINWSVYVYATLT
                     DHVIEASLGYFTNPIVTVLLGVLVLGERLRPMQWIAVGVSGIAVLVLAIGYGSFPWIA
                     LTLAFSFGLYGLVKRRVGPRADAIGGLTVETAVLTPLAVVALIVIASTTGLTMGAVSP
                     LHTVLLASVGVATAVPLLLFAAAARRIPLVYLGFAQYLAPALQLGIGVVLLHEPMPIE
                     RWVGFAIVWVALAILTVDMFRHRYSSQRTRKDEP"
     gene            6583..7842
                     /locus_tag="PLW15_04740"
     CDS             6583..7842
                     /locus_tag="PLW15_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33741.1"
                     /translation="MTRLLVARSVAIGAAMLLLAGCSAPAPAPTPSATVAAPAPTGDG
                     VLKIGTILPTSGTYAFIGAAQIAGVATAVKDINEAGGVNGVPVELLQRDSGDASSSKA
                     EDALKDLVGNAVDVVIGPSSSVQAQRLIPLAAQDKVTMISPAATFPGLSDLPGGRYFF
                     RTIPSYGQQGSALGPVISTGGPKKVAVVYVNDELGKAILPTLTDSLTATGSTIVASVS
                     VPLASPDTAKLVEQVTTAAPDVVVLSTAYSSFDLTKDLITKIIAAGYGGSKLWLTSQN
                     AGDYSQALPEKLLTGVNGIIEGVQPSDTFTERLKQTDSGLGQFRYAAEAYDATILAAL
                     AAIVGGDDSGSSVAATLVDVSRGGIKCTSFAECLEVLRTQTDIDYDGVSGSVNFTPSH
                     DVSPAFWGLYTFNSENKFVFSSGVIAQ"
     gene            8022..9281
                     /locus_tag="PLW15_04745"
     CDS             8022..9281
                     /locus_tag="PLW15_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33742.1"
                     /translation="MSVFVKATASRSRSLKTILGGIAIVGASALVLSGCAPAADTGSS
                     SGPREPLTLKIGTILPQSGTLAFLGPPEEAGVALAVKEINEADLGIQVEVEYRDSGDT
                     TTDTATVSVTDLLSLDVSAIVGAASSGVSKTVIDQIVAAGVVQFSPANTSIDFTDYAD
                     DGLYWRTAPSDVLQGEVLGNQIAEDGVQNLGIIELNDSYGTGLTKKITETFEAAGGKV
                     VASPLFNTGDTVFTTQISEVLAANPDAIALVTFDEAKIIVPALQGAGYTGPLYFVDGN
                     LADYSAVFAAGALDGDKGTLPGLDTAKLGDFTDRLLEVNPDLTDFSYAAESYDAVVLL
                     ALAALAANSTDGKAIASKLQEVSGGSGDGTKATDFASAAQIILDGGVVDYDGNSGPIT
                     FDENGDPTEATIGIFQYGPDNTYKRIN"
     gene            complement(9541..>10175)
                     /locus_tag="PLW15_04750"
     CDS             complement(9541..>10175)
                     /locus_tag="PLW15_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33743.1"
                     /translation="GELIGIIGPNGAGKSTLLKAIFGLVKVREGEVLLQGKDITGLKP
                     NKLVTAGVGFVPQTNNVFPTLSIRENLEMGLFQKPKGIKERLAFVVDIFPELEKRLPQ
                     RAGSLSGGERQMVAMSRALMMGPHVLLLDEPSAGLSPVRQDEAFLRVKEINKAGVTTI
                     MVEQNARRCLQICDRGYVLDQGKDAYTGTGRELLNDPKVISLYLGTLGEE"
BASE COUNT         1610 a         3594 c         3272 g         1699 t
ORIGIN      
        1 tcgcgatcaa cgggcgcacc tatcgcatcg ccggccgcat cgcgatggac caggtcgtcg
       61 tcgacgtcgg cgataccccc gtctcggtcg gtgaccgtgc cgtgctgttc ggtgaccctt
      121 cgaccggtgc gccctccgcc gacgagtggg cggccgccgc gggcaccatc aactacgaga
      181 tcgtgacaag gatcggtccg cgggtcgcac ggcggtaccg cggatgagga tcgagacccc
      241 cgaggcgatg gcgctgctgg gtgcccgtat cgctgccgag ctccgcgccg gcgatctggt
      301 gctgctcaac ggcgagctcg gcgcgggcaa gacgactctc acccgcggca tcggcgaggc
      361 cctcggcgtg cggggcgcgg tcacgagtcc gacgttcgtt ctggcgcgca cgcatccgcg
      421 ggtcgaggcc ggcaccgtcg acgacgcacc gctcgtgcac gtcgacgcct acagactggg
      481 cagcgcgatg gagctcgacg atctcgacat tgacttcccg gcatccatcg tcgtcgtgga
      541 gtggggcgcc gaccttaccg acggcatcac cgatgactgg cttcagatcg acatcgtgcg
      601 cccgcacggc ggtgacgagt ccgcggaggt cgagcctcgc gacgtcaccc tgaccggtca
      661 cggcgagcgg tgggggaggt gggtcgatgc tcttggcgat tgacacctcc gctggcacga
      721 gcgtggcgat cgtcgaccgc gacgccgggg tcatcgccga gcactcctcg cccgataccc
      781 gcggccacgc cgagctgatc ggcaccttca tccaggcctg cctcgacgac tggggcggca
      841 gcgtaaccac cttgtccggt gtcgtttcgg gcatgggtcc cgggccgttc accggcctgc
      901 gggtgggcat agcggccgcc cgggcattcg cgttcggcgc aggaaagatc gtggttcccg
      961 tcgtcagcca cgatgcggtc gcattcggcg ccgaggggcc tgtgctggtc gtcaccgacg
     1021 cccgccgcca cgagctcttc tggagcacct attcgggctc ggatgcctcc ggcctccccg
     1081 tgcgcacgtc cggccccaac ctggccaagg ccgccgagct cgagcacgac gttcccggct
     1141 acgacggcta tcgcctgctc gacagcggac tggtctccgc ggcatccctg ggcctgatcg
     1201 ccgagctgca gtacgcccac ggtcgcccct tcgcggccga tcagccgctg tacctccggt
     1261 cgcccgacgt gacgctgtcg gccggcccga agcgggtcac gtcgtgaccg cctggcagct
     1321 gcgacaggcg acggtcgacg acctcgacgc gatcatgcag ctcgagaccg cgctgttcga
     1381 gtctgacgcg tggtcccggt cgagcatgaa agcagagctt gccgacaggc acgggtacta
     1441 cctcgtggcg ttccccgtgg ggcagcccga gcaggtcgac ggctatgcgg ggctgcgcgc
     1501 gccgcgcggc gaggcccagg ccgacatcca gaccatcgga gtggccgagc acgcgcggcg
     1561 ccagggcctg ggtcgcgtgt tcatgctgcg cctcatcgat gaggctcgcg agcggggggc
     1621 cactgaggtc ttcctcgagg tgcgcgcgga caaccccggg gcccaggcgc tctacgactc
     1681 gctcggcttc gagcgcatcg cggtgcgtcc gaagtactac cagcccgacg gcgtggatgc
     1741 gatcatcatg cgtctgacga tcccggcgcc gcagatggga caggcatgat tgcgcccctg
     1801 gtgctcggca tcgagacgag ctgcgacgag accggcatcg ggatcgttcg cggcacgacc
     1861 ctgctcgcca acgtgatcgc atcgtcgatg caggagcacg cgcgatacgg cggagtcgtg
     1921 cccgaggtcg cggcccgcgc ccacctcgag gcgctcaccc cgaccctgcg cgaggccctc
     1981 gaccgggccg gcgtcgccct tggtgacatt gacgccgtcg ccgtcacgag cggtcccggg
     2041 ttggcgggtg ctctcatggt cggcgtcggc gcggccaagg cgctcgccct cgcgctcgac
     2101 aagccgctgt atgccgtcaa ccaccttgtg ggccacgtag gggccgacgt gctcgacgag
     2161 cggcccctcg acctacccac catcgccctg ctcgtctcgg gcggccacac ctcgctgctg
     2221 ctcgtgcgcg acctcgtctc cgacgtcgaa ctgctcggcg agacgatcga cgacgccgcg
     2281 ggagaggcgt tcgacaaggt cgctcggctg ctcggcctgc cctaccccgg cgggcccgag
     2341 atcgaccgtg ccgccgtcgg cggcgatccg acggcgatcc ggttcccgcg ggggctcagc
     2401 ctgcccaagg acatggagaa gcaccgctac gacttctcgt tctccggcct gaagaccgcc
     2461 gtcgcccgct gggtcgagcg cgaacgcgac gccggtcacg agctgcccct cgcggatgtc
     2521 gccgccagct tccgtgaggc cgtcgttgac gtgctgctca ccaaggccct cgctgcctgc
     2581 cgcgacctgg acgtgccccg cctgctgctc ggaggaggag tcgtcgcgaa tgcccgggtg
     2641 cgcgagctgg ccgcgcagcg gtgcgcggcc gcgggcgtcg agctgcgaat tccgccgctt
     2701 gcgctgtgca ccgacaacgg cgccatgatc gccgcgttgg gagcacagct cgtgatggcg
     2761 ggtcgggtgc catccgggct gggattcggt gccgactcga cgttgccggt cacgacgatt
     2821 caggcctagc gccgtgtggt aacacgccgg aacccgcgtt gacagggtgt gagccgactg
     2881 tcactatggc accgccgtca caacaatctt ctaaggagtt ccatcatgac cgaacccacc
     2941 cccgcaccga gctacgcgcc tgccgccaag tggaacgtgc tgtccatcgt ttcgctggtc
     3001 acggcgatcg tcggcctcgg cctcgtcgcc gtcatcaccg gacacatctc gctcagccag
     3061 atcaagaaga ccggcgagca gggcaacatc ctcgcgatca tcggcttgat cctcggttac
     3121 ctcgagatcg tcggtgtgat catcttcggc atcgtgtgga tcggcatcat cgccgccgcg
     3181 ggcacggcag ggatgtacag caactagcgt catcgccgaa ggggctcacg gcgaaagccg
     3241 cgggcccctt ccgtgcgtta aaatcgacct accgatcaag gagagaccat ggctgacaag
     3301 cccgccgaca acgtaccccc ggtacccgta cccccgagca gcgccgtgcc gcccgtcgag
     3361 cccgcgccga aggtgcgcaa gaccgcagcg cccaagacca ccgccacggc ggctgcggcc
     3421 gcgacaccgg ccaaggctgc cgctccgaag gcgaccccgg cgcccaaggc gactgctgcg
     3481 gccaagaccc ctgcggccaa gacccccgcc gcacccaagg cgcctgcggc cgcggtcgtg
     3541 cccccggctc cgccggtcgc ggtagcgccg cccgcgccgc ctgcaccgcc cgccgcgcag
     3601 acgccgcccg cgtatgcccc gtctgcctac acgcccgctc ccgcaggccc cgcacagggt
     3661 ctcagcatca cctcgatggt gctgggcatc gccgggctct tcctgggctt cctcgtctcg
     3721 atcgctgcag tgatcaccgg acacatggct cagaagcgcc agccctacgc caagccgttc
     3781 tggctcaccg gaatcatcac gggctacatc ggaatcgccc tgggactcat cgtcaccatc
     3841 gcgttcatcg cgtggatcgc cttcatcgcc tcggtcgccg gcgctggcgg gtactacaac
     3901 tactagagcc gttcggcgag cagcgcgcgc ctcttcccgc ccacccgggt gaggaagagc
     3961 gttgcgctgt tctcgccctt cggcgacagg gcggtacgga acgcggcggg atcgatgtcc
     4021 acgccgcgtt tcttgatctc caccgtgccg atgccccggg acgccagctc gcgcttcaac
     4081 gaacgctggt cgagcgcgaa gtccgcgacg atgcgaaacc cttgcgcgaa gggtgtctcg
     4141 gcaggctcgt cagtcgtcag gtacgcgatc gtgcggtcga gcatccgccc accgattagg
     4201 cggccgaggt ctccgatcag tcgggcccgg atgacggcac cgtcgggctc gtagaggaac
     4261 tgtccgaggc tgccgaccgg cacatcctcg ctgtcggatt ctgcggtgag ctccgcggcg
     4321 ccgtgctggc cgatcacgag cgctgctcgg cgcacgttct gtcgcgcgag ggtgccgaac
     4381 cacaggccga cctcaacgac gtcgtgatcg accgataccc actgcgcctc cgcgtcgttc
     4441 gggatcaggt cccgatcgat tccggggccg agcttgaccc cgacgggcag ccgctgggcc
     4501 agaccgtacg cgaagtcgag gctgggcgac cagtccgccg ggttcttcag gcgcttcacg
     4561 ccgtcgcggc gggccggatc gaggtacacc ccgtcgacgc cggccaggtc gaagctctcg
     4621 gcgtcggcat gctcgacccg cgcgttcgac cacggcgcca ggttgtaggc ggcgatcgcc
     4681 gcagtaacct cgtcgcgttc cacggcggtc acctcgagct cgagagcggc gatcgcgagg
     4741 gcatccgcac ccactccgca gccgaggtcc gcgatccagt tgagaccggc gcgctggaac
     4801 cggcccgcgt gctgcgccgc gacgctgagt ctggtggctt gctcgaggcc cgcctcggtg
     4861 aagagcatcg acgcggcgaa cggcccgaac ttggcctcag cccgcgctcg cagcttcgac
     4921 tggttgagca ccgcagcgac cagacccgcc ggatgcccgg ccgcacgcaa gtcgctgacg
     4981 acgcgcacga catccgccgt cgactcatac cggggtagtg cgtcgagcag ccgcagtcct
     5041 tccacggaca ggatctgctc cacctcgtcc cgttccatgg ccccacggta ccggggatcg
     5101 gggctgccgg tgatcgagcg tgtcgagatc tggccgtgcc ggtgatcgag ctcgtcgtga
     5161 tctggcactc acattgatcg agtgccaacc aacccgtata gttgcattgg cactctcaga
     5221 gcgagtgtgc caatccagaa gcgtcaagaa tctaaagact tagaaagagg tcaaccgtgt
     5281 cggtgtccat caagccgctc gaagatcgca ttgtcatcaa gcaggtcgag gccgagacga
     5341 ccacagcgtc gggtctggtc atccctgata ccgccaagga gaagccccag gagggcgagg
     5401 tcgtggccgt tggacccggc cgcatcgacg acaacggcaa tcgcatcccg ctcgacatcg
     5461 ccgtgggcga caaggtcatc tactccaagt acggcggcac cgaggtgaag tacggcggcg
     5521 aagacctgct cgtgctctcg gctcgcgacg tgctcgcggt ggttgtgcgc taacagcgtc
     5581 gactttcggt cagagggccc ggtggcactg ccatcgggcc ctctgctgtt cccggcggcc
     5641 ctaggctgaa acggtgcccg cctcagtccc gtcccacgag cgggtctctg cccccggtat
     5701 cgcctttgcg atcgcgtcct acctgctctg gggcgtccta cccctgctgt tcctcgccct
     5761 gccgcccacg ggggcacccg agatcgtcgc ctggcgcatc gtgctctcgc tggcgttctg
     5821 cgtaatcctg ctgaccgtca cgggcgggtg gtcgcgattc atggcgctcg tgcgcgaccg
     5881 cgtcgcgctg ggcacactcg ccctggccgc cgttctcgtg ctcatcaact ggtcggtgta
     5941 cgtctacgcc accctcaccg accacgtcat cgaggcgagt ctcggctact tcaccaaccc
     6001 catcgtcacg gtgctcctcg gcgtgctggt gctgggggag aggctgcggc cgatgcagtg
     6061 gatcgccgtc ggcgtcagtg gcatcgccgt tctcgtgctc gccatcggct acgggtcatt
     6121 tccctggatc gccctgaccc tcgccttcag cttcggcctc tacgggctgg tcaagaggcg
     6181 ggtgggtccc cgagccgacg ccatcggcgg cctcaccgtc gagaccgcgg tgctcacgcc
     6241 tctcgccgtt gtggcattga tcgtcatcgc atcgaccacc gggctcacca tgggggcggt
     6301 gtcccccctc cacaccgtgc tcctggccag cgtgggagtc gcaacagcgg tgcccctgct
     6361 gctgttcgcc gccgccgcac gcagaattcc gctggtgtac ctcgggttcg cgcagtatct
     6421 cgcacccgct ctgcagctcg ggatcggtgt cgtcctcctc cacgagccga tgccgatcga
     6481 gcgttgggtg ggattcgcga tcgtctgggt tgctctggcg atcctcacgg tcgacatgtt
     6541 ccgccacagg tattcttcgc agagaactcg gaaggacgag ccatgacccg actcctcgtc
     6601 gcccgcagcg tcgcgatcgg tgctgcgatg ctgctgttgg ccggatgctc cgcgcccgcc
     6661 cctgcgccga cccccagtgc cactgtggcg gcacccgcac ccacgggcga cggcgtactc
     6721 aagatcggaa cgatccttcc caccagcggc acctacgcct tcatcggggc ggcccagatt
     6781 gcgggggtcg cgacggcggt caaggacatc aacgaggcgg gaggcgtcaa cggcgtgccc
     6841 gtcgagctcc tgcagcgcga ctcgggcgac gcgtccagca gcaaggcgga ggacgcgctc
     6901 aaggacctgg tcggcaacgc tgtcgacgtc gtgatcggac cctcgtcgtc ggtacaggcg
     6961 cagcgactca tcccgttggc ggcacaggac aaggtgacca tgatctcgcc cgccgcgacc
     7021 ttcccggggc tctcggacct gccgggcggt cgctacttct tccgcacgat cccgagctac
     7081 ggacagcagg gtagcgccct gggtccggtg atctcgaccg ggggcccgaa gaaggtggcc
     7141 gtcgtgtacg tcaacgacga actcggcaag gcgatcctgc cgactctgac cgacagcctc
     7201 acggcgaccg gcagcacgat cgtggcatcg gtcagtgtgc ccctcgcgag ccccgacacg
     7261 gcgaagctgg tcgaacaggt cacgactgcg gcgccggatg tcgtcgttct gtcgaccgcc
     7321 tacagttcct tcgacctcac caaagacctg atcacgaaga tcatcgccgc gggatacgga
     7381 ggatcgaaac tctggctgac ctcccagaac gccggtgact actcccaggc cctccccgag
     7441 aagctgttga ccggcgtcaa cggcatcatc gagggggtcc agccgagcga cacattcacg
     7501 gaacgcctca agcagaccga ctccgggctc ggccagttcc gctacgccgc agaggcctac
     7561 gacgcgacaa ttctcgcggc gctcgccgcg atcgtcggcg gtgatgattc cggcagttcc
     7621 gtggcggcga cgctcgtcga tgtgtcgcgc ggcggcatca aatgcacgag ttttgccgaa
     7681 tgcctcgagg ttctgcgcac gcaaacggac atcgattacg acggtgtgtc cggctcggtc
     7741 aacttcacgc cgagtcacga tgtgtcgcca gcattctggg gcttgtacac gttcaacagc
     7801 gagaacaagt tcgttttttc tagcggagtc atcgcgcaat agggctcggg aatgctccga
     7861 cgagcaggaa gtaacgcagc gacacgaaag gtaacgatcg ggctgcgtta cagggctgta
     7921 acacgctcgt attgagaaag cccccaatcc cgcataacgt gacaggacag cactgcttcg
     7981 ccgtgccgtg tcccaacaca tttcaaaatg caaggagcaa catgagcgta ttcgtgaagg
     8041 ccacagcctc gcgctcgcgg tctctcaaga cgatcctcgg tggcatcgcc atcgttggtg
     8101 ctagcgcact cgtcctcagc ggctgcgcgc cggccgctga caccggatcg tcgtcgggtc
     8161 cccgtgagcc gctgaccctg aagatcggca ccatccttcc gcagagtgga accctcgcgt
     8221 tcctcggccc gcccgaggag gccggtgttg ctctcgctgt caaggagatc aacgaagccg
     8281 acctgggtat tcaggttgag gtcgagtacc gcgactccgg tgacaccacg accgacaccg
     8341 ccaccgtttc ggtgaccgac ctcctctcgc tggatgtctc ggccatcgtt ggtgcggcat
     8401 cctcgggtgt ctccaagacg gtcatcgacc agatcgtggc cgctggcgtc gtgcagttct
     8461 cgccggccaa cacctcgatc gacttcaccg actacgccga tgacggcctc tactggcgca
     8521 ccgctccctc ggacgtgctc cagggcgagg tgctcggcaa ccagatcgcc gaggacggcg
     8581 ttcagaacct cggcatcatc gagctgaacg actcctacgg caccggcctg accaagaaga
     8641 tcaccgagac cttcgaggct gccggtggca aggttgtcgc gtcgccgctg ttcaacacgg
     8701 gtgacaccgt cttcaccacg cagatctccg aggtcctggc ggctaacccc gacgcgatcg
     8761 ccctcgtcac cttcgacgag gcgaagatca tcgttcccgc gctgcagggc gccggctaca
     8821 ccggcccgct gtacttcgtt gacggaaacc tcgctgacta cagcgcggtc ttcgctgccg
     8881 gagcgctcga cggcgacaag ggcaccctgc ccggtctcga caccgccaag ctcggtgact
     8941 tcaccgaccg cctgctcgag gtcaaccccg acctgaccga cttctcctac gcagccgagt
     9001 cgtacgacgc agtcgtgctc ctcgcgctcg ccgctctggc ggcgaactcg accgatggca
     9061 aggccatcgc gtcgaagctt caggaggtct cgggtggttc gggtgatgga acgaaggcga
     9121 ccgacttcgc gtcggccgct cagatcatcc tcgacggtgg cgtagtggac tacgacggaa
     9181 actccggccc gatcacgttc gacgagaacg gtgacccgac cgaggcgacc atcggtatct
     9241 tccagtacgg tcccgacaac acctacaagc gcatcaacta gtttctagct gacacgcagg
     9301 aagggctccg accgtacggt cggggccctt tcgcgttgct ggcgcacccc gcgcgcgtcc
     9361 gtgcccgttc ccgccgccgg ccccgcatcc cgtagccacg agccgtgagg tgcgtcgatc
     9421 gccccttgta ggggccccag aagggcagat tggcgcatct cagcgcgggc cgtggggcat
     9481 tcgacacaca agaggggccg ggatgcagca tcccggcccc tcagtcgtgt gtgagagctg
     9541 ttactcctcg cccagtgtgc ccaggtagag cgagatgacc ttgggatcgt tcaggagctc
     9601 gcggcccgtg ccggtgtacg cgtccttgcc ctggtcgagc acgtagccgc ggtcgcagat
     9661 ctgcaggcag cgacgcgcgt tctgctcgac catgatggtc gtgacgcccg ccttgttgat
     9721 ctccttgacc cgcaggaacg cctcgtcctg gcgtaccggc gagaggcccg ccgatggctc
     9781 atcgagcagc agcacgtgcg gacccatcat gagcgcgcgg ctcatggcga ccatctggcg
     9841 ctcaccaccc gacagcgagc cggcccgctg gggcaggcgc ttctcgagct cggggaagat
     9901 gtcgaccacg aaggcgagcc gctccttgat gcccttcggc ttctggaaca ggcccatctc
     9961 gaggttctcg cgaatgctca gcgtggggaa cacgttgttg gtctgcggca cgaatccgac
    10021 gcctgcagtc accagcttgt tgggcttgag accggtgatg tccttgccct ggagcagaac
    10081 ctcgccctca cgcaccttca cgagcccgaa gatcgccttg agcagcgtcg acttgccggc
    10141 gccgttcggg ccgatgatgc cgatcagctc gccct
//
LOCUS       DAOSOU010000057        10155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6612_length_10155_cov_6.139703, whole genome shotgun sequence.
ACCESSION   DAOSOU010000057 DAOSOU010000000
VERSION     DAOSOU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6612_length_10155_cov_6.139703"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLW15_04755"
     CDS             <1..1082
                     /locus_tag="PLW15_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT33744.1"
                     /translation="FSDAEVMILDEPTNHLDADSVVWLREFLKNYSGGFIVISHDVEL
                     VGETVNKVFYLDANRQQIDIYNMGWKHYLRQREADEERRKKERVNVEKKATQLQLQAA
                     RFGAKASKAAAAHQMVARAEKMLSGLEEVRSVDRVAKLRFPEPAACGRTPLQAHNLSK
                     SYGSLEIFTAVDLAIDRGSKVVVLGLNGAGKTTLLRMLAGVDKPDTGQIEPGHGLRIG
                     YYAQEHETIDVKRSVLENMVSSSPNLTETEARRVLGSFLFTGDDAVKPAGVLSGGEKT
                     RLALAMIVVSGANVLLLDEPTNNLDPASREEILGALASYTGAVVLVSHDEGAVEALNP
                     ERVLILPDGVEDHWNKDYADLISLA"
     gene            complement(1134..1976)
                     /locus_tag="PLW15_04760"
     CDS             complement(1134..1976)
                     /locus_tag="PLW15_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HOT33745.1"
                     /translation="MTGWGFALSRRWLGYLALVVVFAIACTLLSLWQIARRDEARADI
                     TRVDTNWEAEPRPITELLPELDAFDPDLKWSPVSLTGRYLVDEQLLARGRPLDGNPGF
                     EVLVPFQLDDGTVFIVDRGWIPAGNEQDTPDSVPAAPQGVITVVARLKASEPELAGRS
                     APAGQVATINLPTIADLVGKPTYTGAYGILDSEDPAPATRPAAVVKPAPDEGPHLSYA
                     FQWVLFAVMAFIALGWAIRQEYRIRNADDPAERERAAERERKRAAKPRTDAEVEDELL
                     DANR"
     gene            complement(1973..2191)
                     /locus_tag="PLW15_04765"
     CDS             complement(1973..2191)
                     /locus_tag="PLW15_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33746.1"
                     /translation="MTERCSRAGCTSEALWAVNWRNPRIHGADRVKVWLACDEHVEFL
                     RDYLASRDFPVVVTDAAAPLERLPEVTA"
     gene            complement(2188..2472)
                     /locus_tag="PLW15_04770"
     CDS             complement(2188..2472)
                     /locus_tag="PLW15_04770"
                     /inference="COORDINATES: protein motif:HMM:NF022742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HOT33747.1"
                     /translation="MKQPQSITTLAESPDDDRRRRMIRYLIAMTIRVICIILCLFVQG
                     WWLLLPVAGAVILPYIAVVLANVGSGGEAGSVLRPGALVPVAKHGQGAGE"
     gene            2555..3265
                     /locus_tag="PLW15_04775"
     CDS             2555..3265
                     /locus_tag="PLW15_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HOT33748.1"
                     /translation="MTTPRTVLITGGNRGIGRAIAEEFVAQGHRVAVTARSGEGPAGS
                     LTVIADVTDADSIDRAFSEVEAALGPIEVVVANAGITRDTLLLRMSDEDFTDVIDTNL
                     SGAFRVVKRASKGMLKARFGRVILVSSVVGLLGSAGQINYSASKSGLIGIARSLTREL
                     GSRGITANVVAPGFIETDMTAELAPEQQEAYLKQIPAGRFASPEEVAKVISWLASDDA
                     GYISGAVIPVDGGLGMGH"
     gene            complement(3276..3914)
                     /gene="serB"
                     /locus_tag="PLW15_04780"
     CDS             complement(3276..3914)
                     /gene="serB"
                     /locus_tag="PLW15_04780"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOT33749.1"
                     /translation="MARFLVLLDVDSTLIENEVIELLAAEAGSLAEVESITFRAMNGE
                     LDFEESLRARVATLKGLPVSVFEIVARHVTVTAGVDELIATVRAASGRIAVVSGGFHE
                     VLDPIAERLGLDYWRANRLEVADGLLTGGLTGPIIDAQAKADTLREWAADSEVPLSRT
                     VAVGDGANDLPMMAIAGLAVGFDAKAPVRDLAGVLIDARDLSQVLPLMGLRG"
     gene            complement(3937..5178)
                     /locus_tag="PLW15_04785"
     CDS             complement(3937..5178)
                     /locus_tag="PLW15_04785"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT33750.1"
                     /translation="MAPQKVFGIVLAGGEGKRLMPLTADRAKPAVPFGGGYRLIDFAL
                     SNLINSGLRQIVVLTQYKSHSLDRHVSQTWRLSGLLDAYVASVPAQQRLGKRWFSGSA
                     DAILQSLNLVHDEKPDIVVVVGADHVYRMDFSQMVDAHIASGLGLTVAAIRQPIALAD
                     QFGVIEVEAKDPTRIAAFHEKPQKPVGLADSPGEVLASMGNYVFDADVLIDAVIRDGE
                     RSDSNHDMGGDIVPDFVSRGLAGVYDLNRNQVPGSTDRDRFYWRDVGTIESFYDAHQD
                     LISALPIFNLYNRDWPIFSQQLNSPPAKFVRDARGYLGTMIDSMVSLGSLLSGAHIER
                     SVLGPWTTVESGAMVVDSVVFERARIMPNAVVRKAILDKDVVVEAGATVGVDHDADRA
                     RGFTVTETGITVVGKGVRVTP"
     gene            5260..6441
                     /gene="glgA"
                     /locus_tag="PLW15_04790"
     CDS             5260..6441
                     /gene="glgA"
                     /locus_tag="PLW15_04790"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOT33751.1"
                     /translation="MRVDLISREYPPEVYGGAGVHVAELVRALRDRLDVVVRCFGMPR
                     DEADVYSYLVPPELTSANPSLATLGVDLQIAQDVAGADLVHSHTWYANAAGHLAGLLH
                     GIPHVVTAHSLEPLRPWKAEQLGGGYRVSSWIEKTAFEGAAAIIAVSEGMRRDILRSY
                     PSLDEAKVSVVHNGIDLSRWQSVDDPELVRGLGIDPDRPSVVFVGRITRQKGLPYLLR
                     AAALLPPEVQLVLCAGAPDTPEILAEVQAGVAALQAERSGVVWLDTLLSQHELSAVLT
                     QATTFVCPSVYEPLGIVNLEAMACSAPVVGTATGGIPEVVDDGVTGRLVPIEQLQDGT
                     GTPVDPDRFVADLAAALTEVVSDPAAAKRMGEAGRLRAERDFSWTTIAERTTEIYRGL
                     N"
     gene            6454..7236
                     /locus_tag="PLW15_04795"
     CDS             6454..7236
                     /locus_tag="PLW15_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33752.1"
                     /translation="MASVLQFSDVSVVRDGNAILDHVTWSVDAAQRWVILGPNGAGKT
                     TLLQLAAAQMHPTSGRVQVLDDTLGKTDVFELRPRIGFASTALARRVPRDEVVLNVVL
                     TAAYSVTGRWNESYDEIDERRAQRVLGEWKLGALEGRRFGDLSDGEQKRVQIARSVMT
                     DPELLLLDEPAASLDLGAREELLQLLGGYASAASAPAIIMVTHHVEEIPPGFTHALLL
                     ADGAITAAGAINEVITAENLSATFGLPLAVTNDNGRFTARAA"
     gene            7350..7616
                     /locus_tag="PLW15_04800"
     CDS             7350..7616
                     /locus_tag="PLW15_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOT33753.1"
                     /translation="MKTDIHPAYNAIVFRDLASGATFLTKSTATSDKTIELDGETYPV
                     IDVEISSESHPFYTGKQRIMDSAGRVEKFNSRYAGFGTKKDSSK"
     gene            complement(7687..8244)
                     /locus_tag="PLW15_04805"
     CDS             complement(7687..8244)
                     /locus_tag="PLW15_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT33754.1"
                     /translation="MVDLYGTGTLSLEALSTAPAEAFAVPSALATSAPPSAYASAPPP
                     AQPRKPTVLRVAAGIITDETGRTLLVRKVGSSAFMQAGGKIEPGESALDALSRELREE
                     IGLELDPDLTEYLGSFRAVAANEPDTVIRAEVFALVTTGDFAASGEIEELVWIEDPVS
                     HGLELAELTRDTILPLWVERRSALF"
     gene            complement(8323..9006)
                     /locus_tag="PLW15_04810"
                     /pseudo
     CDS             complement(8323..9006)
                     /locus_tag="PLW15_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202509.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(9046..>10155)
                     /locus_tag="PLW15_04815"
     CDS             complement(9046..>10155)
                     /locus_tag="PLW15_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT33755.1"
                     /translation="WMDLGVDGIRLDAIPYLFESEEGNGEGEPKTHEFLKEIRAMVDR
                     DYPGRVLIAEANQWPSEVVEFFGTEEEPECHMAFDFPVMPRIFYSLRSQNASELVRVL
                     SETHDVPSAAAWGVFLRNHDELTLEMVSEEYRQAMYGWYAYDPRMRSNVGIRRRLAPL
                     LDNSRAELELAHALLFSLEGSPFLYYGDEIGMGDNIWLPDRDASRTPMQWTPDRNAGF
                     SSADPGKLYLPVVQSLAYNYTSINVEAQLAQSRSLLNWVRNVIHVRKGHPTFGLGTLR
                     VLATNHESVLAFVREYPGSSTQFGDSAETVLCVFSFAHSPVSARIDLTGFEGATAFDL
                     FGGAEFPAVGADGTLSLTLGTQGFYWLHLGDAPRA"
BASE COUNT         1750 a         3363 c         3446 g         1596 t
ORIGIN      
        1 tgttctccga cgcagaggtg atgatcctcg acgagccgac caaccacctc gacgcggact
       61 cggtcgtgtg gttgcgcgag ttcctcaaga actactccgg tggattcatc gtgatcagcc
      121 atgatgtcga gctggtgggg gagaccgtca acaaggtctt ctacctcgac gcgaatcggc
      181 agcagatcga catctacaac atgggctgga agcactacct gcgccagagg gaagccgacg
      241 aggaacgccg caagaaggaa cgcgtcaacg tcgagaagaa ggccacccag ctgcaactgc
      301 aggcggcacg cttcggcgcc aaggccagca aggccgctgc tgcgcaccag atggtggcgc
      361 gcgccgagaa gatgctctcc ggactcgagg aggtgcgcag cgtcgatcgc gtggccaagc
      421 tgcgcttccc cgagccggcg gcctgcggtc gcacgccact gcaggctcac aacctctcga
      481 agagctatgg atcactggag atcttcaccg ccgtcgacct cgcgatcgat cgcggctcca
      541 aggtcgtcgt gctcgggctc aacggtgcag gcaagaccac gctgctgcgg atgctcgccg
      601 gcgtcgacaa gcccgacacc ggccagatcg agccggggca tggcctgcgc atcggttact
      661 acgcccagga gcacgagacc atcgacgtca agcgctcggt gctcgagaac atggtctcgt
      721 cgtcaccgaa cctcaccgag accgaggcgc gtcgggtgct cggctcgttc ctgttcaccg
      781 gggatgacgc ggtcaagccc gcgggtgtgc tgagcggcgg cgagaagacc cgtctggccc
      841 ttgccatgat cgtcgtgagc ggagccaacg tactgctgct cgacgagccc accaacaacc
      901 tcgacccggc cagccgcgag gagatcctcg gtgcgctggc gagctacacc ggcgccgtgg
      961 tgctcgtgag ccacgacgag ggggctgtcg aggcgctcaa ccccgagcga gtactgattc
     1021 tgcccgacgg cgtcgaggac cactggaaca aggattacgc ggacctgatc agcctggcgt
     1081 agacctcgac gggctcgatc accgggggcg ctcgatcagc gcgacgggct cgatcagcgg
     1141 ttggcgtcga gcaactcgtc ctcgacttcg gcgtcggttc gtggcttcgc tgccctcttg
     1201 cgctcgcgct cggccgcccg ctcccgctcg gccgggtcat ctgcgttgcg gatgcggtac
     1261 tcctgacgga tcgcccagcc gagagcgatg aatgccatga cggcgaacag cacccactgg
     1321 aaggcgtagg agaggtgcgg cccctcgtcg ggcgcgggct tgacgaccgc ggccggccgc
     1381 gtcgccggcg ccgggtcctc cgagtcgaga attccgtagg cacccgtgta ggtgggtttg
     1441 ccgacgaggt cggcgatcgt gggcagattg atcgtcgcaa cctgacccgc gggtgccgag
     1501 cgcccggcca gttcgggttc actcgccttg agacgcgcaa ccacagtgat gacaccctgc
     1561 ggtgccgccg gaacggagtc cggagtgtcc tgctcgttgc cggcgggaat ccagccgcgg
     1621 tcgacgatga agacggttcc gtcatcgagc tggaacggca cgagcacctc gaaacccggg
     1681 ttgccgtcga gcggccgacc gcgcgcgagc agctgctcgt cgaccagata gcggccagtg
     1741 agcgaaaccg ggctccattt caggtcgggg tcgaaggcgt cgagttcggg cagcaactcg
     1801 gtaatcgggc gcggctcggc ctcccagttc gtgtcgacgc gggtgatgtc ggcgcgagcc
     1861 tcgtcgcggc gcgcgatctg ccagagcgac aggagtgtgc acgcgatggc gaagaccacc
     1921 accagtgcga ggtatcccag ccaccggcgt gacagcgcaa agccccagcc ggtcacgcgg
     1981 tgacctcggg gaggcgctcg aggggcgccg cggcatccgt cacaaccacc gggaagtcac
     2041 ggctcgcgag atagtcgcgc aggaattcga cgtgctcgtc gcacgcgagc cagaccttga
     2101 cccgatcggc tccatggatg cgcggattgc gccagttgac cgcccagagg gcctcactgg
     2161 tgcaccccgc acgtgaacac cgttccgtca ctcgccagct ccctggccgt gcttcgccac
     2221 cggaacgagc gcgccggggc gcaggaccga acccgcctcg ccgcccgaac cgacgttcgc
     2281 gagaacgacg gcgatgtacg gcaggatcac ggcgcccgca acgggcagaa gaagccacca
     2341 gccctgcacg aacaggcaca ggatgatgca gatcactcgg atggtcatgg caatgaggta
     2401 gcggatcatc cggcgacgac gatcatcgtc gggcgactcg gcaagagtgg taatggactg
     2461 cggctgcttc atggctcacc tggctgtaga gggttgaatt cggctcctca ccctccagcc
     2521 tacgtcgctt gagcctgact ataggctggt gcgcatgaca acccccagga ccgtactgat
     2581 caccggtggc aatcgaggaa tcgggcgtgc aatcgccgag gagttcgtgg cccagggtca
     2641 ccgggtcgcg gtcaccgcgc gctccggcga gggcccagcg ggaagcctca cggtcatcgc
     2701 cgacgtcacc gacgccgaca gcatcgatcg cgccttctcc gaggtggagg ccgcactggg
     2761 gccgatcgag gtcgtggtcg ccaacgcggg catcacccgc gacaccctgc tgctgcgcat
     2821 gtccgacgag gacttcaccg acgtcatcga caccaacctc agcggggcgt tccgcgtcgt
     2881 caagcgggcg tcgaagggga tgctgaaggc ccggttcggc cgcgtcatcc tggtctccag
     2941 cgttgtcggc ctgctcggtt cggccggcca gatcaactac tccgcgtcga agagcgggct
     3001 gatcgggatc gcgcgctcgc tcacgcggga actcggaagc cgcggcatca ccgcgaatgt
     3061 cgtcgctccc ggattcatcg agaccgacat gacggccgag ctcgcgcccg agcagcagga
     3121 ggcctacctc aagcagatcc cggcgggtcg gttcgcgagc ccggaggagg tcgcgaaggt
     3181 gatctcctgg ctcgcgagcg acgatgccgg gtacatctcc ggtgcggtca ttcccgtcga
     3241 cggcggcctc gggatggggc actgagcgga agcgatcagc cccgcagtcc catcagcggc
     3301 agcacctgcg agaggtcgcg cgcgtcgatg agaacccccg cgaggtcgcg cacgggcgcc
     3361 ttcgcgtcga agccgacggc gaggccggcg atggccatca tgggcagatc gttggcgccg
     3421 tctccgaccg cgacggtgcg cgagagcggc acctcggagt ctgccgccca ctcgcgcagc
     3481 gtgtcagcct tcgcctgcgc gtcgatgatc gggccggtca agccgcccgt gagcagaccg
     3541 tcggcgacct cgagacggtt ggcgcgccag tagtccagac ccagccgctc cgcgatcgga
     3601 tcgagtacct cgtggaagcc gccggacacg acggcgatgc gccccgaggc cgcgcgaacg
     3661 gtcgcgatca gctcgtcgac accggccgtt accgttacgt gccgggccac aatctcgaac
     3721 accgatacgg ggagaccctt cagggttgca acccgagcgc gaaggctctc ctcgaagtcg
     3781 agctcgccat tcatcgcacg gaacgtgatc gactcgacct cggcgaggga gcccgcctcg
     3841 gcagcgagca actcgatcac ctcattctcg atgagcgtcg aatcgacatc gagcagcacc
     3901 aggaagcggg ccaacgcggt cctttcctga acatacctac ggggtgacgc gcacgccctt
     3961 gccaacaacg gtgatgccgg tctcggtgac tgtgaagccg cgggcgcgat cggcgtcgtg
     4021 atcgacgccc accgtcgcgc cggcctcgac cacgacatcc ttatcaagaa tagccttgcg
     4081 aacgaccgcg ttcggcatga tgcgggcgcg ctcgaacacg accgagtcga ccaccatggc
     4141 acccgactcg acggtcgtcc agggcccgag aacgctgcgc tcgatgtgag cgccggagag
     4201 caaggaaccg agcgacacca tcgagtcgat catggtgccg agatagcccc gggcatcgcg
     4261 cacgaacttc gccggcggcg agttgagctg ctggctgaag atcggccagt cgcggttgta
     4321 gaggttgaag atcggcagag ccgagatcag gtcctggtgg gcgtcgtaga acgactcgat
     4381 ggtccccacg tcacgccagt agaagcgatc gcggtccgtc gatcccggca cctgattgcg
     4441 attgaggtcg tagacccccg cgagaccgcg cgagacgaag tcgggaacga tatcgccgcc
     4501 catgtcgtgg ttggagtcgc tgcgctcccc gtcacggatg accgcgtcga tgagcacgtc
     4561 ggcgtcaaac acgtagttgc ccatggatgc cagaacctcg ccaggggagt ccgcgagccc
     4621 gacgggtttc tgcggcttct cgtggaaggc ggcgatccga gtcggatcct tcgcctccac
     4681 ctcgatgacc ccgaactggt cggcgagggc gatcggctgc cggatggcgg cgacggtcag
     4741 tcccaggccc gacgcgatgt gcgcatccac catctggctg aagtccatgc ggtagacgtg
     4801 gtcggcgccg acgaccacga cgatgtcggg cttctcgtcg tgcaccaggt tgaggctctg
     4861 gaggatggcg tcggcggagc cgctgaacca gcgctttccc aggcgctgct gggcgggcac
     4921 cgaggcgaca tacgcgtcga gcagcccgga cagccgccag gtctgcgaca cgtggcggtc
     4981 aaggctgtgg gacttgtact gagtgagcac cacgatctgg cgcagccccg aattgatgag
     5041 gttgctcagg gcaaaatcga tcaggcggta gccgccaccg aagggcacag cgggcttcgc
     5101 ccgatccgcc gtcagcggca tgagacgctt gccctcgccg ccagcgagca cgattccgaa
     5161 gaccttctgt ggtgccataa gccaacagta ggcgacatgg cgtgccgggg cgagagacac
     5221 cgggccgcgc gcacgatggc cgtgcagtaa cgtcatggca tgcgcgttga cctgatcagt
     5281 cgtgaatatc cgcccgaggt ctacgggggt gctggcgttc acgtcgccga gctcgtacgg
     5341 gcgcttcgtg accggctcga cgtcgtggtt cgctgcttcg ggatgcctcg ggacgaagcc
     5401 gatgtctact cctatctcgt gcctcccgag ctgacgagcg ccaacccctc ccttgccacc
     5461 ctgggtgtcg accttcagat cgcacaggat gtggccggtg ccgatctcgt gcactcgcac
     5521 acctggtacg ccaatgcggc cgggcatctg gctggcctcc tgcacgggat acctcacgtg
     5581 gtcaccgccc acagtctgga gccgttgcga ccgtggaagg ccgagcagct cggcggggga
     5641 taccgggtct cgagctggat cgagaagacc gcgttcgagg gcgcggcggc gatcatcgcg
     5701 gtgagcgagg ggatgcgccg cgacatcctg cgctcgtatc cgagcctcga cgaggccaag
     5761 gtctccgtcg tgcacaacgg catcgacctc agccgatggc agtcggtgga tgaccccgaa
     5821 ctcgtgcgcg gcctgggcat cgaccccgac cgtccgtcgg ttgtcttcgt gggcaggatc
     5881 acccgccaga agggtctgcc ctacctcctt cgcgcagcgg cactgctgcc tccggaggtt
     5941 caacttgtcc tgtgcgccgg ggctcccgac accccggaga tcctcgccga ggtgcaggcg
     6001 ggagtggccg cacttcaggc cgaacgctcc ggtgtcgtgt ggctggacac acttctgagc
     6061 cagcacgaac tgtcggcggt gctgacccag gccacgacgt tcgtctgtcc ctctgtctac
     6121 gagccgctgg gcatcgtcaa cctggaggcc atggcgtgct cagcgccggt cgtcggcacc
     6181 gcaacggggg gaatacccga ggtggtcgat gacggggtca ccggtcggct cgtgccgatc
     6241 gagcagctcc aggacggaac gggaactccg gtcgaccccg accgtttcgt cgctgacctc
     6301 gccgccgctc tcactgaggt cgtgagcgac cccgctgcgg cgaagcgcat gggcgaagcc
     6361 ggccgtctga gggcggaacg ggacttcagc tggacgacca tcgccgagcg cacgacggag
     6421 atctaccgcg gactcaatta ggctggacga accatggcca gtgtcctgca gttctccgac
     6481 gtttcagttg tccgtgatgg caacgccatc ctcgatcacg tcacctggtc ggttgatgcc
     6541 gcccagcggt gggtgatcct cggaccgaac ggcgccggca agacgacgct gctgcagctc
     6601 gcggccgcgc agatgcaccc caccagcgga cgcgtgcagg tgctcgacga cacgctcggc
     6661 aagaccgacg tgttcgagct gcgcccccgc atcggtttcg cctctaccgc gctggcgcgt
     6721 cgcgtgccgc gggacgaggt cgtgctcaat gtcgttctca cggccgccta ctcggtgacc
     6781 ggccgatgga acgagagtta cgacgagatc gatgagcgcc gcgcccagcg cgtgctgggg
     6841 gagtggaagc tcggcgcgct ggagggccgc cgattcggtg acctcagcga tggcgagcag
     6901 aagcgggtgc agatcgcccg atcggtcatg accgatccgg agctcctgct gctggacgag
     6961 cccgcggcaa gcctcgacct cggcgcacgc gaggagctgc tgcagcttct cggagggtac
     7021 gcgagcgcgg catcggctcc cgcgatcatc atggtgacgc accacgtcga ggagatcccg
     7081 cccggtttca cgcacgccct gctgctcgcc gacggcgcga tcacggctgc gggtgccatc
     7141 aacgaggtca tcaccgcgga gaacctcagc gcgacattcg ggctgcccct ggccgtcacc
     7201 aacgacaacg gacggttcac tgcccgcgca gcgtgacccc gtagccccga tctgataaga
     7261 tcgacagtcg gcccagtgca cgaacggact gcgtcgcccc ggactttaca gctcagcttc
     7321 acacctcacg ccaatcccaa ggaatcacca tgaagaccga catccacccc gcgtacaacg
     7381 ccatcgtgtt ccgcgacctc gcgtcgggtg ccacgttcct caccaagtcg actgcgacca
     7441 gcgacaagac gatcgagctc gacggcgaga cctacccggt catcgatgtc gagatctcgt
     7501 cggagtcgca cccgttctac acgggcaagc agcgcatcat ggactccgct ggccgcgtcg
     7561 agaagttcaa ctcgcgctac gccggtttcg gcaccaagaa ggactcctcg aagtagggtc
     7621 ctcacctcgg cttcaccgcg aagggcgatc gggaaaccgg tcgcccttcg tcgttgacgg
     7681 gttcggctag aacagcgccg aacggcgctc cacccacagc ggcaggatgg tgtccctggt
     7741 gagttccgcc agctcgagcc cgtgggacac cggatcctcg atccatacca gctcctcgat
     7801 ctccccggag gcggcaaagt cgcccgtggt gaccagggcg aaaacctcgg cgcggatgac
     7861 cgtgtccggc tcgttcgcgg cgacggcgcg gaaactcccg aggtactcgg tcaggtcggg
     7921 gtcgagttcg agtccgatct cctcacgcag ttcgcgggac agggcatcca gcgcgctctc
     7981 gcccggctcg atcttgccgc cggcctgcat gaacgcggag ctgccgacct tgcgcaccag
     8041 gagggtgcgg ccggtctcgt cggtgatgat tcctgcggca accctcagca cggtcggctt
     8101 gcgcggctgt gccggaggag gagcggatgc gtacgccgag ggcggcgcgg acgtcgcgag
     8161 cgcagaggga acggcaaacg cctctgccgg ggccgtcgac agcgcctcaa gcgagagcgt
     8221 acccgtgccg tacaggtcga ccaccggaac ggtcaggctc ggtcgcggtt ccggtgcggg
     8281 gatgggctgg gtgtcgatga gggatcgcgg gtgctcgctg ggtcgcagcg gccatgcggt
     8341 gtctgcgacg tacgcgccgt cgcccttcac cgatcggatg tactcctgga agctcgccgc
     8401 ctgctcggcg taccagacct cctggcggcc gtggaggtcg gcggtaggga cgcccagttc
     8461 ctcgggatag gcccggagga gggcctgcgc gacccggccg gcggcgatcg cgtcggaccc
     8521 ggcatcatgt gcgtcgtcca gtggtacccc gtagcgctct gcggtgacct ccagggtgcg
     8581 cttgcccttg cggtagcggt ccatcgcctt gtcgagcacg agcgggtcga tgacggggga
     8641 ggtgacttcc agcggtcgga gctggtggcg gcggcactct cggtcgagca gggtgaggtc
     8701 gtagggggcg ttgtagacga ccacgggcat cccgagcgag aacagcacgc gcagtgtctg
     8761 ggcgatctcg gcgaccacct gtactgcggg acgacccagt gcgcgggcac gctcggtggt
     8821 gatgccgtgc actgccgagg cgccctcggg aatctcgatg ccggggtcgg cgagctagtc
     8881 ccagcggtcg acgacctcgc cgtcgtcgtc gatcacggcg atgcacgcgg aaacaatgcg
     8941 actcgtgtcg acatcgacac ccgtcgtttc gaggtcgaag accccgagcc ggccccagtt
     9001 ggtcatgctc tcagggtagg tggcacagcc gacagacccg cgcgactacg ctcgcggcgc
     9061 gtcgccgagg tgcagccagt agaagccctg cgtgcccaga gtcagcgaca gtgttccgtc
     9121 tgctcccacg gcggggaatt cggcgccacc gaacaggtcg aaggcggtcg caccctcgaa
     9181 gccggtgagg tcgatccgtg ccgagaccgg gctgtgggcg aagctgaaca cgcagagcac
     9241 cgtctcggcc gagtcgccga actgcgtgct cgagcccggg tactcccgaa cgaaggcgag
     9301 caccgactcg tggttggtcg ccagcacccg cagggtgccg agaccgaacg tcgggtgccc
     9361 cttgcgcacg tggatgacgt tgcgcaccca gttgagcagc gaacgcgact gcgcgagctg
     9421 cgcctccacg ttgatggacg tgtagttgta ggcgagggac tgcacgacgg gcagatagag
     9481 cttgcccggg tcggcggacg agaagccggc gttgcggtcg ggagtccact gcatgggggt
     9541 gcggctggcg tcccggtcgg ggagccagat gttgtcgccc atgccgatct cgtcgccgta
     9601 gtacaggaac gggctgccct cgagggagaa caacagcgcg tgggcgagtt cgagttctgc
     9661 ccgcgaattg tcgagcaggg gagcaaggcg acgccggatg cccacgttgg agcgcatccg
     9721 tgggtcgtag gcgtaccagc cgtacatcgc ctgccggtac tcctcgctca ccatctccag
     9781 ggtcagctcg tcgtggttgc gcaggaagac accccacgcg gctgcgctcg gcacgtcatg
     9841 ggtctcggag agcacgcgca cgagctccga cgcgttctgc gatcggagcg agtagaagat
     9901 tcggggcatc accgggaagt cgaaggccat gtggcactcg ggctcctcct cggtgccgaa
     9961 gaactcgacc acctcgctgg gccactggtt ggcctcggcg atgagcaccc gtcccgggta
    10021 gtcccggtcg accatcgccc ggatctcctt gaggaactcg tgggtcttcg gctcgccctc
    10081 accgttgccc tcctcggact cgaagaggta cgggatcgcg tcgagacgga tgccgtcgac
    10141 tccgagatcc atcca
//
LOCUS       DAOSOU010000058        10150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6615_length_10150_cov_7.793561, whole genome shotgun sequence.
ACCESSION   DAOSOU010000058 DAOSOU010000000
VERSION     DAOSOU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6615_length_10150_cov_7.793561"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PLW15_04820"
     CDS             <1..1407
                     /locus_tag="PLW15_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HOT33756.1"
                     /translation="AEHLAQLEEWMRSYRPEELFDGDGRPVASLGVLRPSGIQRMSAL
                     PQANGGELLVPLVLPPIEEHEVDISKGHGVTAEATRVLGRWLADVMDANDNFRMFGPD
                     ETASNRLDAVLEVTSKQWEADIVATDQHLADEGKVIEILSENLVQGLLEGYLLTGRHG
                     MMNSYEAFIHVIDSMFNQHAKWLEACNEIAWRRPVASLTYLLSSHVWRQDHNGFSHQD
                     PGFLSVVMNKSAKVVRVYLPPDANTLLVTAEHCLGSRNYVNVIVAGKQPAPTFLSIEE
                     ARVHGARGLSIWDWAGTEVPGEAPDVVVASAGDVPTMEALAAVRLLADAIPELRIRFV
                     NVVDLMRLQPKEEHPHGSSAADFDAFFTTDKPVIFAFHGYPALIHQLAYRHTNHANIH
                     VRGFKEKGTTTTPFDMLMMNDLDRYRLAMDVIRRVPGLEERHASLVQHFADERARMRA
                     YAYENGEDSPEVTGWDWL"
     gene            complement(1531..4794)
                     /gene="cydC"
                     /locus_tag="PLW15_04825"
     CDS             complement(1531..4794)
                     /gene="cydC"
                     /locus_tag="PLW15_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HOT33757.1"
                     /translation="MRSSRALLPRRFVYLVGALSGAKALSLVMLAECIARGISAIAAG
                     DPGWSGVLGWAAAAALLRAGSAWATSVVATRAALDTKQRLRAQLADVLVSGTPRVGSS
                     AALATRGLDDLDEYFTSVLPAITSVAVVPLVVIARVVFADWLSAVILVLTVPLIPVFM
                     VLVGQYTKQKTDAAASALDRLSDHVVELARGLPVLVGLGRADDQAKALRGISDDYRKR
                     TLATLRTAFLSSLVLELIATISVAVVAVTIGVRILDGGMTLEVGLLVLILAPECYTPF
                     REFGAAYHASRAGVAAFERVTAIVDAPLAVRAVVPASTVSASHFTVTYPTGTVVAPVS
                     FSLPPVGITALLGASGTGKSTLLRALAGRLDAEAIVSGELAGIDADRIAWAPQHPRAV
                     ESTVRAELELYALGLPVDDLLARLGLAVVADSDPAELSPGELRRLAVARAALRVRSGA
                     TLLLLDEPTAHLDDHWAAVVQRLVAELAETCSVFVASHDTAVTSIATGAIRLGTPGPL
                     RERAEDGTVSEGRARQHVATERVEPREIARAWNEFVRPAMGRYVGSAVLGTLAAGFAI
                     ALTAVSAWLIVRASEQPAIMYLLVAIVGVRFFGLGRAGLRYAERLVTHDAAFTSATAL
                     RLRLWAALSARAGSSRALQRGGTALDYLVGATDEIRDLVPRVLVPGAVGVVVSIGAVI
                     TVGLILPTALAVIVATLMVALLMAPLIALAADRAAERDRQRMRSGVLRRFVAFLDAAD
                     DLRGNGVDGIVRAELAEADQRVSAFSRRVAASRGLGLATVLLACGLGTVGMLAVGSSA
                     VASGAIGVELVAVLALLPIALADSLAGFVAAMQLVPALVTALTRVARMREPATDPHRT
                     ATLDSPVTDLRLTRLAATWPGATSPAFEHVSADAKRGDWLVVSGESGTGKSTLLSVLL
                     GQLAPSDGWLEFNGRDIAELDPVQLRRHVSWCPQDSHLFDSTIRANLLIARSRDDAPS
                     ETELTEALTRAGLSQLIATLPDGLDTRIGSQGSHLSGGERQRLAVARTLLSRSEIVLL
                     DEPTAHLDEDTADQLMDDLRVAFADRIVVLVTHHHGDTRLGDQVLRLGSQQSAPVPA"
     gene            complement(4798..5826)
                     /gene="cydB"
                     /locus_tag="PLW15_04830"
     CDS             complement(4798..5826)
                     /gene="cydB"
                     /locus_tag="PLW15_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOT33758.1"
                     /translation="MDVLPTIWFVAIGALWIGYLVLEGFDLGVGMHMLFTGRSDAQRR
                     VMLNTIGPVWDGNEVWLLTAGAATFAAFPLWYASLFSTLYLPLVLVLLGLIFRAVSIE
                     YRGKGATERWTRFWDFSIGIGSLVVAFGIGAALALTTTGLPINENGDRVGGPFVWLNG
                     YAILGGLAVIGFCLVHGAAFLALKTDGPIREKTGKFLVRWSPVFLLPIVAWVLIIQFQ
                     HGTILSWALVVIAVVAVVIAWLSARAGREGRAFTGIAVFVAAGAGAIFAAVYPVVLPS
                     TLDPAFSLTVSNASSGDYTLMVMSIVTGIFLPVVIAYQAWTYWVFRKRISETHIPTKH
                     VVVAAVGK"
     gene            complement(5848..7410)
                     /locus_tag="PLW15_04835"
     CDS             complement(5848..7410)
                     /locus_tag="PLW15_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOT33759.1"
                     /translation="MDPVDIARWQFGITTVYHFLLVPLTLGLGPTVAIMQTMWVRTGN
                     ERYLRMTKFWGKLYLINFIMGVATGIVQEFQFGMAWSEYSRFVGDVFGAPLAMEGLLA
                     FFIESTFLGLWIFGWNRLPKRLHLATLWLAVLGSIVSAFFIIAANSWMQHPVGIEFID
                     GRPVLTDIFAVLSNNTAIAAYTHTIFGALGVGGAFLLGIAWYQLWQRRKDNIDTVDAA
                     GRVVVGENRTIPGRDKADHSVWITSLRWGAVVAIIGFAGVAITGDYQAKLMFEQQPMK
                     MAAAEAACADGTGFSVLAFGDLAAQDCSQVTTVIEIPGLLSFLANGDFTTEVKGVNTL
                     VPIYQDKYGTNLPDNPILGDRAGQAIDYVPLMAVTYWGFRLMIGFGALAAFAAVVALW
                     MTRKGTVPKSKWLMRLALLGILAPFAASAAGWVFTEMGRQPFVVAPNPTPGGIDGVFM
                     FTASAVSPGVEPWEMIVSLVALTLVYLALLVVEVGLLWKYARAGVAGVMPELDESHDD
                     TDKTDDVLAFAY"
     gene            7551..7988
                     /locus_tag="PLW15_04840"
     CDS             7551..7988
                     /locus_tag="PLW15_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT33760.1"
                     /translation="MSTHAMRAMLPPNAEGKSGAIALVSSDDCWRLLAKASLGRIAVQ
                     TTDGLDIFPINYVVTGEEIFFRSAPGSKLVDIAANPVVAFEVDGRHGGHRWSVVVKGT
                     AHRLDRDDMIESSGVLRLPTATSTEKFNYVRIIPTSISGRRIR"
     gene            8038..8433
                     /locus_tag="PLW15_04845"
     CDS             8038..8433
                     /locus_tag="PLW15_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33761.1"
                     /translation="MSDSEKSAFTIDLSGVETPAGKVGRVLIGALLLVVFGVLALACG
                     VLYFFGAFIPGVSDAWAGWLVDLAPFAGAIFVGLAIVGFVVMRKGRNSEQAVDTIVNV
                     LEATGLADVETDEGPSSTLPGKPLPPTVV"
     gene            8801..9067
                     /locus_tag="PLW15_04850"
     CDS             8801..9067
                     /locus_tag="PLW15_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33762.1"
                     /translation="MNTTLSTAPGRHDHPPQPVLQPAALPIRRVSLLDRAALTVGIAL
                     IKWGRRPLAIESRERRASRIEQQLARLERERTFERQARLTLPVR"
     gene            complement(9125..9832)
                     /locus_tag="PLW15_04855"
     CDS             complement(9125..9832)
                     /locus_tag="PLW15_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33763.1"
                     /translation="MNTLLDSALYAAQRTLGSLRGVIPSLRWTRKHLPIAATAAVFAL
                     VAVSSLAPPRWPTPAEDFQAYIDQHAQTLSVPSTVLAASVTRDDYSATPGIATLAAGG
                     TNYDWAKLVLLLAGFPMTESNVTVFTRWMRQENGPDDWWNRNNPLNNGWGSGGGGGLG
                     SYDNLVIAAENAAAALRTNSGYAGIVDAFASGAPTDVIESAIWASPWASGHYADGGHW
                     AYTPVPVVTAPTDAWGQ"
     gene            9983..>10150
                     /locus_tag="PLW15_04860"
     CDS             9983..>10150
                     /locus_tag="PLW15_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT33764.1"
                     /translation="MRASDRAYSALRSEILEWHLAPGTVLGEVEQAARLGVSRTPVRE
                     ALARLSADGLVE"
BASE COUNT         1752 a         3425 c         3509 g         1464 t
ORIGIN      
        1 gccgagcacc tcgcccagct cgaggagtgg atgcgcagct accgccccga ggagttgttc
       61 gacggcgacg gccgcccggt ggcgagcctc ggggtgctgc ggcccagcgg catccagcgc
      121 atgagcgcgc tgccgcaggc caacggcggg gaactgctgg ttcccctcgt gctgccgccg
      181 atcgaggagc acgaggtcga catctcgaaa ggccacgggg tcaccgccga ggcgacccgc
      241 gtgctcggcc gctggctggc cgacgtgatg gacgccaacg acaacttcag gatgttcggc
      301 cccgacgaga ccgcctccaa ccggctcgac gccgtgctcg aggtgacctc gaagcagtgg
      361 gaggccgaca tcgtggccac cgaccagcat ctcgccgacg agggcaaggt gatcgagatc
      421 ctcagcgaga acctcgtgca gggactgctc gagggctacc tgctcaccgg tcgccacggc
      481 atgatgaact cctacgaggc gttcatccac gtgatcgact cgatgttcaa ccagcacgcc
      541 aagtggctgg aggcgtgcaa cgagatcgcc tggcggcggc ccgtcgcatc cctcacctat
      601 ctgctcagtt cgcacgtctg gcgacaggac cacaacggct tctcgcacca ggaccccggc
      661 ttcctcagtg tcgtcatgaa caagagcgcc aaggtcgtgc gggtctacct gccaccggat
      721 gccaacactc tgctcgtaac cgccgagcac tgcctgggca gccgcaacta cgtgaacgtg
      781 atcgtggccg gcaagcagcc ggcgccgacg ttcctgtcga tcgaggaggc ccgggtgcac
      841 ggtgcgcgag gcctcagcat ctgggactgg gccggcaccg aggtgcccgg cgaggcaccc
      901 gatgtggtgg tcgcgagtgc tggcgatgtg cccacgatgg aggcgctcgc ggccgtgcgg
      961 ctgcttgccg acgcgatccc cgagctgcgc atccgcttcg tcaatgtcgt cgacctgatg
     1021 aggctgcagc cgaaggagga gcacccgcac ggctcatcgg cggccgactt cgacgcgttc
     1081 ttcaccaccg acaagcccgt gatcttcgcg ttccacggct acccggcgct gatccaccag
     1141 ctcgcctacc ggcacaccaa ccacgccaac atccacgtgc gcggcttcaa ggagaagggc
     1201 accaccacga cgcccttcga catgctcatg atgaacgacc tcgaccggta ccgcctggcc
     1261 atggatgtca tccgccgggt tcccgggctc gaggagcgtc acgcgagcct cgtgcagcac
     1321 ttcgccgacg aacgcgcccg catgcgcgcc tacgcctacg agaacggcga ggacagcccc
     1381 gaggtcaccg ggtgggactg gctgtagatc gcgaggcgcc cccgcagttg cggaggatga
     1441 cacgcgggcc gcggcctgcg ccggctcggc gccgcggcgc gccgtcctcc gtacgtgcgg
     1501 tggccgaagt gccgggcgcg cgcccggccg ctacgccgga acgggcgctg actgctgcga
     1561 cccgaggcgc agcacctggt cgccgaggcg cgtgtcgccg tggtggtgcg tgaccagcac
     1621 gacgatgcgg tcggcgaagg ccacccgcag gtcgtccatg agctggtcgg cggtgtcctc
     1681 gtcgaggtgg gccgtcggct cgtcgagcag cacgatctcc gagcgcgaca gcagtgtgcg
     1741 ggccacggcg aggcgctgac gctcgccacc ggagaggtgc gagccctgcg aaccgatgcg
     1801 cgtgtcgagc ccgtcgggca gggtggcgat gagctgggac agtccggccc gggtcagggc
     1861 ctcggtcagc tccgtctcgc tcggtgcgtc gtcgcgcgag cgggcgatca gcaggttggc
     1921 gcggatcgtc gaatcgaaca ggtgcgaatc ctgcgggcac cacgacacgt ggcgacgcaa
     1981 ctgcaccggg tcgagctccg cgatgtcgcg cccgttgaac tcgagccacc cgtcgctcgg
     2041 cgcaagctgg ccgagcagca cgctgagcag agtcgacttg ccggttccgg actcgcccga
     2101 gacgacgagc cagtccccgc gcttggcgtc cgcagacacg tgctcgaagg cgggcgaggt
     2161 cgcaccgggc caggtcgcgg cgagccgagt gaggcgcaga tcggtcaccg gactgtcgag
     2221 ggtcgccgtg cggtgcgggt ctgtcgcggg ttcgcgcatc cgggcgacac gagtgagcgc
     2281 ggtgaccaac gcgggaacca gctgcatcgc agccacgaac cccgcgagcg agtcggcgag
     2341 agcgatcggt agcagtgcga ggacggcgac gagctcgaca ccgatggctc cggatgccac
     2401 ggcgctcgat cccacggcga gcatgccgac cgtgcccagc ccgcacgcga gcaggacggt
     2461 tgcgagcccc agacctcggg atgccgcgac ccgccggctg aatgcgctga cccgctgatc
     2521 tgcctccgcg agttcggcgc gcacgatgcc gtcgacaccg ttgccacgca ggtcatcggc
     2581 cgcgtcgagg aacgcgacga accgccgcag cacgccggag cgcatccgct gccggtcgcg
     2641 ctcggcagca cggtcggcgg cgagcgcgat caggggcgcc atgagcaggg cgaccatgag
     2701 ggtcgccacg atcacggcga gcgcggtggg caggatgagt ccgacggtga tgacggctcc
     2761 gatggagacc acgacgccga ccgcgccggg cacgagcacc cgcggcacaa ggtcacggat
     2821 ctcgtcagtc gccccgacga ggtaatcgag cgctgtgccc ccgcgctgca gcgcgcggga
     2881 actgccggcg cgagcggaga gcgctgccca gagccgcagg cgcagagcag ttgcggaggt
     2941 gaaggctgcg tcgtgggtga ccaaccgctc cgcgtagcgc aggcccgcgc ggccgagtcc
     3001 gaagaaccgc acgcccacga tggcgaccag aaggtacatg atggcgggct gctcgctggc
     3061 acgcacgatc agccaggccg acacggcggt gagggcgatc gcgaagcctg cggccagagt
     3121 tcccagcacg gccgagccga cgtagcggcc catcgcgggg cgaacgaact cgttccaggc
     3181 ccgcgcgatc tcccggggtt cgacccgttc ggtcgcgaca tgctgacgcg cgcgcccctc
     3241 gctcaccgtg ccatcctcag cacgctcgcg cagcggtccc ggcgtaccca accggattgc
     3301 accggtcgcg atcgaggtca ccgccgtatc gtgcgacgcg acaaacacgc tgcaggtctc
     3361 ggccagctcg gcaacgaggc gttgcacgac ggcggcccag tggtcgtcaa ggtgtgcggt
     3421 cggctcatcg agcagcagaa gcgtcgcacc cgagcgcacc ctcagtgccg cgcgtgcgac
     3481 cgcgagccga cgcagctcgc cggggctcag ctcggcgggg tcggagtcgg cgaccacggc
     3541 caggccgagt cgagcgagca ggtcatcgac cggcaggccg agcgcgtaga gctcgagctc
     3601 cgcgcgcacc gtgctctcga ctgcccgggg atgctgcgga gcccacgcga tccgatcggc
     3661 gtcgatgccc gcgagctcgc ccgacacgat cgcctcggca tcgagccggc ccgcaagcgc
     3721 gcgcagcagt gtgctcttgc cggtgccgct cgccccgagc aacgcagtga tcccgaccgg
     3781 cggcagagag aaactcaccg gagcgacaac cgtgccggtg gggtaggtga cggtgaagtg
     3841 cgacgccgac accgtcgacg caggtaccac cgcgcgcacg gcgagcggcg cgtcgacaat
     3901 ggcggtgacc cgctcgaacg ctgcgacacc cgcccgggac gcgtggtacg cggccccgaa
     3961 ctcgcggaac ggcgtgtagc actcgggcgc aaggatcagc acgagcaggc cgacctcgag
     4021 agtcatgcca ccgtcgagaa tgcgcacgcc gatcgtcact gccaccacag cgaccgagat
     4081 ggtcgctatc aactcgagca cgagcgacga caggaacgcg gtgcgcaggg tcgcgagagt
     4141 gcgcttgcgg tagtcgtcgg agatgccgcg cagcgccttc gcctgatcgt cggcgcgccc
     4201 cagtcccacc agcacgggaa gcccgcgcgc caactcgacc acgtggtcgc tgaggcggtc
     4261 gagggcggat gccgcggcat ccgtcttctg cttcgtgtac tgccccacga gcaccatgaa
     4321 caccggaatg agcggcaccg tgagcacgag gatcacggcg ctcagccagt cggcgaacac
     4381 gacccgcgcg atcaccacga ggggcacgac ggccaccgag gtgatcgcgg gcagcacact
     4441 cgtgaagtac tcgtcgaggt catcgagccc gcgcgttgcc agagccgccg atgagccgac
     4501 ccggggagtg cccgagacca gcacatcggc cagctgcgcc cgcaggcgct gcttggtgtc
     4561 gagtgcggcc ctcgtggcga ccacggaggt cgcccacgcg gagcccgcgc gcagcagcgc
     4621 ggccgccgcc gcccagccca gcacgccgga ccacccgggg tcccccgccg cgatggcgga
     4681 gatcccccgg gcgatgcact cggcgagcat gaccagtgac agcgctttcg cgccgctcag
     4741 tgcaccgacc aggtacacga agcgacgcgg aagcagggct cgcgacgacc tcatcagcta
     4801 ctttccgacg gcagccacga ccacgtgctt ggtcgggatg tgcgtctcgc tgatgcgctt
     4861 gcggaagacc cagtacgtcc aggcctggta ggcgatcacc acgggaagga agatgcccgt
     4921 gacgatcgac atcaccatca gcgtgtagtc accgctcgaa gcgttggaga ccgtgaggct
     4981 gaacgcgggg tcgagcgtcg agggcagcac caccgggtag accgcagcga agatcgcgcc
     5041 agcaccggca gccacgaaga ccgcgatacc cgtgaatgca cggccctcac gacctgcgcg
     5101 ggcactgagc caggcgatga cgaccgccac aaccgcgatg accacgagcg cccagctcag
     5161 gatggtgccg tgctggaact ggatgatcag cacccaggcg acgatcggaa gcaggaacac
     5221 ggggctccag cgaacgagga acttccccgt cttctccctg atcgggccgt cggtcttgag
     5281 cgcgaggaac gcggcaccgt gcacgaggca gaagccgatc accgcgagtc caccgagaat
     5341 cgcgtagccg ttgagccaga cgaacgggcc gccgacacgg tcaccgttct cgttgatggg
     5401 caggccggtg gtcgtcagcg cgagtgccgc gccgattccg aaggccacca ccaacgagcc
     5461 gatgccgatc gagaagtccc agaacctcgt ccagcgctcg gtcgcgccct tgccgcggta
     5521 ctcgatcgac acggcccgga agatcaggcc gagcagcacg agcacgagag gcaggtacag
     5581 ggtggagaac agcgaggcgt accagagcgg gaaggcggcg aaggttgcag cgcccgcggt
     5641 gagcagccag acctcgttgc cgtcccagac cgggccgatg gtgttgagca tcacgcggcg
     5701 ctgggcgtcg ctacggcccg tgaacagcat gtgcatgccg acacccaggt cgaatccctc
     5761 gagcacgagg tatccgatcc acagggcgcc gatggcgacg aaccagatgg tgggcagaac
     5821 gtccatgatt gaaagtctcc tagcggctta gtaggcgaac gcgagcacgt cgtcggtctt
     5881 gtcggtgtcg tcgtggctct cgtcgagctc gggcatcaca cccgcgactc cggcccgggc
     5941 gtacttccag agcagaccca cttcgaccac cagcagagcc aggtagacga gggtgagggc
     6001 cacgagggag acgatcatct cccacggttc cactccgggc gagaccgctg aggcggtgaa
     6061 catgaacaca ccgtcgatgc cgccgggtgt cgggttgggg gccacgacga agggctggcg
     6121 gcccatctcc gtgaagaccc agccggccgc gctggccgcg aacggagcga ggatgcccag
     6181 cagggcgagg cgcatgagcc acttcgactt gggtaccgtt cccttgcggg tcatccacag
     6241 agcgacgacg gctgcgaagg cggcgagcgc cccgaacccg atcatcagcc ggaagcccca
     6301 gtaggtgacg gccatgagag gtacgtagtc gatcgcctgg ccggccctgt caccgaggat
     6361 cgggttgtcg ggaaggttcg ttccgtactt gtcctgatag atcggcacga gggtgttgac
     6421 acccttgacc tcggtcgtga agtcaccatt cgcgaggaac gagagcagcc cgggaatctc
     6481 gatgaccgtg gtcacctgcg agcaatcctg agcggcaagg tcaccgaagg cgagcaccga
     6541 gaatccggtg ccgtcggcgc aggcggcctc agcggcggcc atcttcatcg gctgctgctc
     6601 gaacatgagc ttggcctgat agtcacccgt gatggcgacc cccgcgaagc cgatgatcgc
     6661 gaccacggca ccccaccgca gcgacgtgat ccagacgctg tggtcggcct tgtcgcggcc
     6721 cgggatggtg cggttctcgc ccacgacgac gcggcccgca gcatctacgg tgtcgatgtt
     6781 gtccttgcga cgctgccaga gctggtacca ggcaataccg agcaggaacg ccccgccgac
     6841 tccgagggcg ccgaagatcg tgtgggtgta ggcggcgatc gcggtgttgt tggagagtac
     6901 cgcgaagatg tcggtgagta ctggacgccc atcgatgaac tcgatgccca ccggatgctg
     6961 catccacgag ttggcggcaa tgatgaagaa tgccgagacg atcgagccca gcacggcgag
     7021 ccagagggtc gcgaggtgca gccgcttcgg cagccggttc cagccgaaga tccacagacc
     7081 gaggaaggtc gactcgatga agaaggcgag cagtccctcc atggccagcg gggcaccgaa
     7141 gacgtcaccc acgaagcggg agtactcgct ccatgccatg ccgaactgga actcctgcac
     7201 gatgccggtg gcgacaccca tgatgaagtt gatcaggtag agcttgcccc agaacttggt
     7261 cattctcagg tagcgctcgt taccggtgcg cacccacatt gtctgcatga tggcgacggt
     7321 cgggccgagg cccagagtga gcggaacgag caggaagtgg tacacggttg tgatgccgaa
     7381 ttgccagcgg gcgatgtcga cgggatccaa ggcagggcct tcctcgtggg agttgattgg
     7441 tcaactccac gattccgctc cgcagacatc acacctaggg tcgaaggtcc cgtggcccgg
     7501 cgggaccttc tgccctatgg ccggtcgggg gttcggcgtt gactgaggtc atgtctaccc
     7561 acgccatgag ggcgatgctc ccgccgaatg cagagggcaa gagcggggcc atcgccctcg
     7621 tgagttctga cgactgctgg cgtctgctgg cgaaggcgag cctcggccgt atcgcggtgc
     7681 agacaaccga cggactcgac atcttcccca tcaactacgt ggtcaccggc gaggagatct
     7741 tcttccgcag cgcgccgggt tccaagctcg tcgacatcgc ggcgaacccg gtcgtcgcct
     7801 tcgaggtcga cggccgccac ggaggccacc gctggagcgt cgtcgtcaag ggcacggccc
     7861 atcgtctcga ccgtgacgac atgatcgaga gctcgggtgt tctgcggctg ccgaccgcga
     7921 ccagcaccga gaagttcaac tacgtgcgca tcatcccgac gtcgatcagc ggtcgccgca
     7981 tccgctagct gacggccctc tggccgtcgc cccatacgcg ccactatggt gtcgggcatg
     8041 agcgattctg agaagtccgc cttcacgatc gacctctccg gcgtggagac gcccgccggg
     8101 aaggtcggac gcgttctcat cggtgcactg ctgctggtcg tcttcggcgt gctcgcgctg
     8161 gcgtgcggcg tgctgtactt cttcggcgcc ttcatccctg gggtgtcgga tgcctgggcc
     8221 ggctggctcg tcgacctggc ccccttcgcc ggggcgatct tcgtggggct cgcgatcgtc
     8281 ggcttcgtcg tgatgcgcaa gggtcgcaac tccgagcagg ctgtcgacac catcgtgaac
     8341 gttctcgagg cgaccggtct ggccgacgtg gagaccgacg aaggcccctc ctcgaccctg
     8401 cccggcaagc cgctgccccc gaccgtggtg tgatcgtcgg tcgttaccat tccgttgtcg
     8461 ccccgggcgt gtcatgggtt gcggcagccg cgacatcgcc gctaccgtga gtcacgcgcc
     8521 acaggggcgc cgcacttcat gccgaaagga ggccgaccgt gttgattgca tttcgaggga
     8581 tgattcccac caccgccggc ttcgtcggcg tcctcgccga ctgatcgggt agctgttcac
     8641 gccgcccgtt gatcggtggc gacgctctcg aagctcgagt gactcccgca cgggcatccc
     8701 gtgtttcgcg gacactcatc tcgccacgca cggcggcgcg ctcactcagc gctgtcgccg
     8761 caccccgcca caagcgaatc ccgtgaggaa gaagatcacc atgaacacca cgctgtcgac
     8821 cgcacccggt cgtcacgacc atccacccca accggtgctg cagcctgcgg cgctgccgat
     8881 ccggcgggtc agcctgctcg accgggcggc gctcaccgtc ggtatcgcgc tgatcaagtg
     8941 gggccgccgg cccctcgcga tcgagtcgcg tgagcgacgt gccagccgta tcgagcagca
     9001 actcgcgcga ctggagcgcg agcgcacctt cgagcgtcag gctcgactca ccctgcccgt
     9061 tcggtgagca gggtaaccgg ccgaggccgt cgcggaatct gtgctccgcg gcggcctctt
     9121 gccgttactg gccccaggcg tcggtcggcg ccgtgacgac cggcaccggc gtgtacgccc
     9181 aatggccgcc gtcggcgtag tgaccggatg cccagggcga ggcccagatc gctgactcga
     9241 tgacgtcggt gggagcgccc gacgcgaacg cgtcgacgat gcctgcgtac ccgctgttgg
     9301 tgcgcagcgc agccgccgca ttctcggccg cgatcacgag gttgtcgtag ctgccgagtc
     9361 ccccgcctcc gccggaaccc cagccgttgt tcaacgggtt gttgcggttc caccagtcgt
     9421 cggggccgtt ctcctggcgc atccaccggg tgaagacggt cacgttgctc tcagtcatcg
     9481 ggaagccggc cagcagcagc acgagcttgg cccagtcgta gttggtgccg cccgccgcga
     9541 gggtggcgat gccgggggtg gccgagtagt cgtcgcgtgt gacggatgcc gcgagcaccg
     9601 tcgatggcac gctcagggtc tgcgcgtgct ggtcgatgta ggcctgaaag tcctccgcgg
     9661 gcgtcggcca gcggggcggg gcgagcgagg agacggcgac gagggcgaac actgccgccg
     9721 tcgccgcgat cggcaggtgc ttgcgggtcc agcgcaggga ggggatgacc ccgcgcagcg
     9781 agccgagggt tcgctgcgcc gcgtagaggg cggagtcgag aagtgtattc atagtgaaaa
     9841 ggggggtcgt ttcggaccgg cagttaccgt aacaggcggg gctgggtcgg ggctgggaat
     9901 cgccgctgta tacattacag ccgaatcggg gtagatttca ctcctaaagg ctcacgaatg
     9961 tatacagagg cggtgagggc acgtgcgggc tagcgatcgc gcgtacagcg ccctgcgctc
    10021 ggagatcctc gagtggcatc tcgcccctgg caccgttctc ggcgaggtgg agcaggccgc
    10081 gcggctcggc gtcagccgca ctcccgtgcg tgaggcgctc gcgcgactca gcgccgacgg
    10141 gctggtcgag
//
LOCUS       DAOSOU010000059        10140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6627_length_10140_cov_7.170253, whole genome shotgun sequence.
ACCESSION   DAOSOU010000059 DAOSOU010000000
VERSION     DAOSOU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6627_length_10140_cov_7.170253"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLW15_04865"
     CDS             <1..1263
                     /locus_tag="PLW15_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33765.1"
                     /translation="AYFRLVRNVVISVRNELYVDAAKVAGLSDARIIGRHVLVVVRAP
                     IIIEMAFVAGFALVIQAGLEFIGLGSPENPTWGGMLQDSFNALYISPLSILWPAAAII
                     LTVLAFVLISNAVRDTLGDPRNSAGRKRRAIQGDVESQAATSDAFLSVNNLSIAYPLS
                     AHETREVVKGVSLEVNRGEVLGLVGESGSGKTQTAFAILGLLPEEAIVASGRITLGGR
                     SLLGLSEKQLNALRGNDIAYVPQEPMTNLDPAFTVGQQFDFAMRKKLKLTKAQTKERA
                     IALLARVNINDPERVHGLYPHQLSGGMAQRVLIAIAVSCDPALLIADEPTTALDVTVQ
                     AEVLDLLRDLQRERSMAMILVTHNFGVVADICDRVAVMKDGRVVETGDTRSIIREPQD
                     PYTQLLLASVLDNAPMRAPLDAEKETAS"
     gene            1260..2129
                     /locus_tag="PLW15_04870"
     CDS             1260..2129
                     /locus_tag="PLW15_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33766.1"
                     /translation="MTGAHETLLSIRDVVIEYKRSGWRAQPNRVLHSVSLDIREGETV
                     GLVGESGSGKTTLGRGILGLVPIASGTVTYDGRDITHVSRRERRGLAKEIQVVFQDPY
                     SSLNPSLLVSDILSEPLRAAGVAGGEARARVKDLLDRVGLPTDASHRYPSEFSGGQRQ
                     RIAIARALALEPRLIVCDEPVSALDLSTQARVLDLLVEIQERTGVALLVISHDLSVVR
                     AVCHRVAVMYHGEIVEQGTTAQVIDDPQHDYTKRLLLAAPVSDPDEQQKRREARRLLN
                     ETALAADRESVGA"
     gene            2122..3327
                     /locus_tag="PLW15_04875"
     CDS             2122..3327
                     /locus_tag="PLW15_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT33767.1"
                     /translation="MPDRIDRLRVESEVPLVLRAGNDRLAVRGADHLEQTDGGLRVHR
                     LPERAIRRIHTEFFRSVEPQASGVRLAARSQASWIELTLRTVALTVEGDDPLSFWGRI
                     DLVIDGRQHGSVALAPGAVRTVDFLTNSSTVTPGEPLTVRFADLPARDKTIEMWLPHD
                     VEVTLIDVRADAPLAVPDDDRPVWVNYGSSISHGFNAAGPTGTWPAVAATSVDVDLVN
                     LGFSGSALLDSFVAEAIRDRPADIISLEIGTNVVNHDAFRVRTFVPAVHAFLDIIRQG
                     HPTSPLWVVSSVLCPIVEDRPGPTTMVGPPGERTAIAEGLPEDLVRGRLSLGLIRDLL
                     AAVVASRAAEDPNLHYLDGRLLYGETEHEAMPMVDGLHPDAPAQLHIGRRFAELVLEP
                     TVNTRERGD"
     gene            3329..4315
                     /locus_tag="PLW15_04880"
     CDS             3329..4315
                     /locus_tag="PLW15_04880"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HOT33768.1"
                     /translation="MSHSVRAVVSARPDQVAVEEISLAPLGADQVMIRTLHSGVSTGT
                     DKWVLQGKFTWGNAQYPLCPGYQRVGVIEELGADVTAFRVGQVVATTTSVGLEGVHPF
                     WGAHIERAASAQGSVYPADGVDPRAASLFVSAQVGYNAASRVTGAPGDGVIVVGDGII
                     GSSAALCARARGFRVLMVGRHDTRLAPLAELGIHTLNSRTGSLDAVASFDPVAAIDTI
                     HSLDSFATYFPALPIRSGQVVFSGHSPGGVTTWGDMEAMQKKELTAHFVSGWTPERLT
                     ATLQLMRSGDLPLERLVGSVARGTEAIVGLLTDVINGGLEPVAATIEWGDPS"
     gene            4312..5235
                     /locus_tag="PLW15_04885"
     CDS             4312..5235
                     /locus_tag="PLW15_04885"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT33769.1"
                     /translation="MKIVITGAAGGIGRRVTAALEQAHDLLLVDSVAPEEATVFDMKS
                     PTGRRLAPLTPNVPYVRADIGEPAALADTFRGADAVIHLAGWPTGEWDNAMQIMTTNV
                     LGTFSVYTAARDAGVRRVVNASSINAFGSFFWRVHPESPVRSRLPLSEDERRVPEDPY
                     SLSKGVTEEIGETFRRAWGIEAVNLRFAAVWSEEKYEELLTTGLPVTEAWADDLFQWV
                     HPNDVRDGIILAATVDQTTSEPIVLAAADTRAPEDTLELIRRFKPELERELREPLPGR
                     SSLLSITRARSFLGFEPRHSFAAAVQSDGQR"
     gene            5378..6379
                     /locus_tag="PLW15_04890"
     CDS             5378..6379
                     /locus_tag="PLW15_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT33770.1"
                     /translation="MTVSVRDVAAAAGVSVGTVSNVLNRPDIVAPATVERVQAAIDSL
                     GFIRNDAARQLRAGKSRTIGMVVLNVGNPFFTDVARGAEVRASAAGMSLLLGNSDEKY
                     DRELAYFDLFEEQRVHGLLVTPVGDDLSRLERISNNGTPVVLVDREADPNAFSSVSVD
                     DVSGGFLATRHLLDTGRRRLAFVGGPASMRQVADRLAGARRAVSEESGATLEHIPTDS
                     MTVEHGRAAGEWIRLRSRSDRPEAVFAGNDLVALGVLQGLMMHNDVRVPEDVALIGYD
                     DIDFASAAVVPLSSIRQPRMLIGHTGVDLLLRLADEGADHERVVFQPELVVRSSTAG"
     gene            complement(6396..7316)
                     /locus_tag="PLW15_04895"
     CDS             complement(6396..7316)
                     /locus_tag="PLW15_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33771.1"
                     /translation="MELSAVGWTTLLLGTPTDRWSHSGIGVLADGRLVISASGGGELL
                     LLDEHTGDIDGVWTGSAHAHGIEAIEGADGNELWIADPGPLGQSGQLTRIRVDGTMLA
                     RLTEPGTDGLAEQWKPTSTAVVTGATAHRGDLWIADGYGRSLVHRVSGTGDTRTIDGS
                     TTGMRFDCPHGIAIDYRGPEPLVAIADRGNQRVIFLTLDGEFVRAVTDDAMPTPSSLA
                     LRGDDLLVTDLRGALLRIDRTDRVHVIVADSHGSDREGWPNRVVDGEVVAPEFREGCL
                     NSPHGLAVGPTGAVYLTEWALGGRVIRLDL"
     gene            complement(7307..8506)
                     /locus_tag="PLW15_04900"
     CDS             complement(7307..8506)
                     /locus_tag="PLW15_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT33772.1"
                     /translation="MTTTPTGCPVHPRVLPTDGTPLEPSPVLGEWREEGPVTPLHYTD
                     GHDGWIVTRHELARRVLEDPRFSQQPQRIPGETIPGGPELPYLDDEALASKRVANLLF
                     LDGEAHSRVRRILMARFSVRAGRAQQPAIAAIVARQVAHLLTVGSPADLMAEFAAPIS
                     AAVHRLILGIPDARADEFARLFLREAPVQDRVLFARAVIEQKRSDLDEDVLSDLIRSD
                     LSAGEVDWVALVLMISGRDAAAYMMATSLLALLRHPDQLAALRDDPDLMGGAVEEFMR
                     YGTMFVALFARTATEDVELGDTRFAAGESVSVSAVAANRDPQRFENPDEFDITRDAFG
                     HLGFGHGIHGCLGQQIARIEIREAVGQLLAALPAVKLVSAEQLNPLPFAHPVATYSAG
                     SVIVEWS"
     gene            complement(8520..9698)
                     /locus_tag="PLW15_04905"
     CDS             complement(8520..9698)
                     /locus_tag="PLW15_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT33773.1"
                     /translation="MTDRAGAGTLILGAGVAGVTTALNLRDDGYEDPVTLVGDEDRAP
                     YSRPPLSKQLLSGRLNEDDIHLCRGDDLQLRDIRYLRGVSASTVDLQARRVLVGADWL
                     PFEHLVIATGVSARRPAGTYPAGSIHTVRTVEDSRSLRAQLDRPGPVAVIGAGVLGLE
                     LAAGIRQLGHDVAVLGRSRHVRLGRTGEHLASLVESVLRENGVDLRLGCEPVGVATTG
                     SVTGVMLADGSVVPATTVVAAVGSTPRVEALVGTDLDISDGVLCDEHGVAAPGVWAVG
                     DVARWYDPDAGLARRVEHQATAIEQALAVARSIATGEPSPPIVPFHWTELFSQRIVVH
                     GRLNADLPLTLLAGSLDDRRFVGATLHDGRPTGLVGWNMPREFRQERARFDDLAPAHR
                     "
     gene            complement(9695..9892)
                     /locus_tag="PLW15_04910"
     CDS             complement(9695..9892)
                     /locus_tag="PLW15_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT33774.1"
                     /translation="MRVNVDTAACIGAGQCALVAPDVFDQDDDGIVMLLNAAPSEPEH
                     AATRRAAALCPARVIVVDESE"
BASE COUNT         1566 a         3473 c         3446 g         1655 t
ORIGIN      
        1 gcgtacttcc ggctggtgcg caacgtggtc atctcggtgc gcaacgagct ctacgtcgac
       61 gcggccaagg tcgccgggct ctccgacgct cggatcatcg gacgtcacgt gctggtggtc
      121 gtccgggctc ccatcatcat cgagatggcg ttcgtggccg ggttcgccct ggtcatccag
      181 gccggcctgg agttcatcgg cctcggatca cccgagaacc cgacctgggg cgggatgctc
      241 caggactcct tcaatgccct gtacatctcg ccgctgtcca tcctctggcc ggccgccgcg
      301 atcatcctca ccgtgctcgc cttcgtcctg atctccaacg ccgtgcgcga caccctgggt
      361 gacccgcgca acagcgcggg acgcaagcgg cgcgcgatcc agggtgacgt ggaatcccag
      421 gccgcgacat ccgatgcctt cctcagcgtg aacaacctct cgatcgccta cccgctctcg
      481 gctcacgaga cgcgcgaggt cgtcaagggc gtcagcctcg aggtcaatcg cggtgaggtg
      541 ctcggactcg tcggcgagag cggctccggc aagacgcaga ccgcattcgc gatcctcggg
      601 ctcctgcccg aggaggcgat cgtcgcgtcc ggtcggatca ccctcggcgg gcgttcgctg
      661 ctcgggctct ccgagaagca gctcaacgcg ctccgcggca acgacatcgc ctacgttcct
      721 caggagccga tgacgaatct cgacccggcc ttcactgtgg gccagcagtt cgacttcgcg
      781 atgcgcaaga agctcaagct caccaaggcg cagaccaagg aacgcgcgat agccctgctg
      841 gcgcgggtca acatcaacga tcccgagcgc gtgcacggtc tctaccccca tcagctgtcg
      901 ggtgggatgg cgcagcgcgt actcatcgcg atcgcggtct cgtgcgaccc cgcgctgctg
      961 atcgccgacg agccgaccac cgcactcgac gtcaccgtgc aggctgaagt gctcgacctg
     1021 ctgcgagacc tccagcgcga gcgttccatg gcgatgatcc tggtcaccca caacttcggc
     1081 gtcgtcgccg acatctgcga ccgggtggcc gtgatgaagg acggtcgcgt ggtggagacc
     1141 ggggacaccc ggtcgatcat cagggagccg caggatccgt acacccagtt gctgctggca
     1201 tccgtgctcg acaacgcccc gatgcgagcg cccctcgatg ccgagaagga gaccgcctca
     1261 tgaccggcgc gcacgagaca ctgctgagca tccgcgacgt ggtgatcgag tacaagcgca
     1321 gcggctggcg cgcacagccg aaccgagtgc tgcactcggt gtcgctcgac atccgcgagg
     1381 gcgagactgt cggcctggtg ggggagtccg gttcgggcaa gaccacgctg ggtcggggca
     1441 tcctcggtct cgtgccgatc gcatccggaa ccgtcaccta cgacgggcgg gacatcacac
     1501 acgtgtcgcg gcgggaacgg cgcggcctgg ccaaggagat ccaggtcgtg ttccaggatc
     1561 cctactcgtc gctcaacccc tctctgctgg tgtccgacat cctctccgag ccactgcggg
     1621 cggcaggtgt cgccggtggg gaggcccggg cgcgcgtgaa ggatctgctc gaccgtgtcg
     1681 gcctgccgac ggacgcatcg caccgctacc ccagcgagtt ctcgggcggt cagcgccaac
     1741 gcatcgccat cgcgcgtgcg ctcgcgctcg aaccccggtt gatcgtctgc gacgagcccg
     1801 tgagcgccct cgacctctcc acgcaggccc gcgtgctgga tctgctggtc gagatccagg
     1861 agcgcaccgg ggtcgcgctg ctggtgatct cacacgacct ctccgtggtc agggcggtct
     1921 gtcaccgtgt ggcagtcatg taccacggcg agatcgtcga gcagggcacc acggcccaag
     1981 tcatcgacga cccgcagcac gactacacga agcgccttct gctcgcggca cccgtgtccg
     2041 acccggacga gcagcagaag cgccgggagg cccgccgact gctcaacgag accgcgctcg
     2101 ccgcggatcg tgagagtgtc ggtgcctgac cggatcgatc gcctgcgagt cgagagcgag
     2161 gttccgctgg ttctgcgcgc cgggaacgat cgactcgccg tgaggggtgc cgaccatctc
     2221 gagcagaccg acggaggtct gcgggtacac cggttgcccg agcgagcgat tcgacgcatc
     2281 cacaccgagt tcttccgatc ggtcgaacct caggcttcgg gggtgcgcct ggcggcgcgg
     2341 tcgcaggcgt cgtggatcga gctgactctt cggactgtcg cgctgaccgt ggagggcgac
     2401 gacccgctgt cattctgggg gcggatcgac ctggtgatcg acggtcgcca gcacgggtcg
     2461 gtcgcactcg caccgggtgc ggttcgcacc gtcgacttcc tgacgaacag ctcgacggtc
     2521 acacccggcg agcccctgac ggtgcggttc gccgatctgc ccgccaggga caagaccatc
     2581 gagatgtggt tgccccacga tgtcgaagtg acgctcatcg acgtgcgtgc ggatgcgccg
     2641 ctggcggtgc ccgacgacga ccggccggtc tgggtcaact acggcagttc gatcagtcac
     2701 ggattcaacg cggcaggacc gaccggtacc tggccggccg tcgcggcgac gtccgtcgat
     2761 gtcgacctgg tcaacctcgg cttcagcggc agcgcactgc tcgactcgtt cgtcgctgag
     2821 gccatccgcg accgtccggc cgacatcatc agcctcgaga tcggcacgaa cgtggtcaac
     2881 cacgacgcct tccgggtgcg cacgttcgtg cccgcggtgc acgcgttcct cgacatcatc
     2941 cggcaggggc atcccacctc gccgctgtgg gtggtctcgt cggtcctctg tcctatcgtg
     3001 gaggatcgcc cgggcccgac gacgatggtc ggaccgccgg gcgagcgaac cgccatcgcc
     3061 gagggactgc cggaggacct ggtgcgaggt cgcctgagtc tcgggttgat ccgcgacctg
     3121 ctcgccgctg tcgtcgcctc gcgggcggct gaggatccga acctgcacta cctcgacggc
     3181 cgcctgctct acggcgagac ggagcacgag gcgatgccca tggtcgatgg cctgcatccg
     3241 gatgcgccgg cgcagctgca catcggacgt cgcttcgccg agctggtgct cgagcccacc
     3301 gtgaacaccc gagaaagggg tgactgacat gtcccattcc gtgcgtgccg tcgtctcggc
     3361 ccggcccgac caggtcgctg tcgaggagat ctccctcgcg ccgctcggcg ccgaccaggt
     3421 gatgatccgg accctgcatt cgggggtgag caccggcacc gacaagtggg tgcttcaggg
     3481 caagttcacg tggggcaacg cccagtaccc gctgtgcccc ggctatcaga gggtcggggt
     3541 gatcgaggag ctcggcgccg acgtcacggc gttccgggtc gggcaggtcg ttgcgacgac
     3601 cacgagcgta ggtctcgagg gcgtgcatcc gttctggggg gcacacattg agcgcgccgc
     3661 ctcagcgcag gggtcggtct accctgcaga cggagtcgac ccccgggccg cctcgctctt
     3721 cgtgtcggca caggtcggct acaacgcggc gtcgcgagtg accggtgcgc cgggcgacgg
     3781 tgtcattgtc gtgggcgacg gcatcatcgg gtcgtcggcc gctctctgcg cgcgagcgcg
     3841 gggcttccgg gtgctcatgg tggggcgtca cgacactcgt cttgcaccgc tcgccgagct
     3901 gggcatccac actctcaaca gtcgaaccgg gtcgctcgac gcggtcgcgt ccttcgatcc
     3961 ggtcgcggcg atcgacacga tccacagcct cgactcgttc gccacctact tccctgccct
     4021 gccgatccgg tcgggtcagg tcgtcttcag cggtcacagc ccgggaggcg tcaccacctg
     4081 gggtgacatg gaggcgatgc agaagaagga gctcacggcg catttcgtgt ccggctggac
     4141 tcccgagcgc ctgaccgcga ccctgcagct gatgcgatca ggcgatctcc ccctggagcg
     4201 gctggtcggc tctgtagcgc gcggtacgga ggcgatcgta ggccttctca ccgacgtcat
     4261 caacggtggc ctcgaaccgg tcgctgcaac catcgagtgg ggggacccgt catgaagatc
     4321 gtcatcaccg gagcagcggg gggaatcggc cgtcgcgtca ccgccgccct cgagcaggcc
     4381 cacgacctcc ttctggtcga ctccgtggcg cccgaggagg ccacggtctt cgacatgaag
     4441 tcaccgaccg ggcggcggct cgcgccgctc actccgaatg tgccctacgt gcgcgccgac
     4501 atcggcgagc ccgcagcact ggccgacacc ttccgtggcg ccgacgctgt catccatctg
     4561 gccggctggc ccaccggtga gtgggacaac gcgatgcaga tcatgaccac gaacgtgctc
     4621 ggcacgttct cggtgtacac cgccgcgcgg gatgcggggg tgcggcgggt ggtcaacgcc
     4681 tcgagcatca acgccttcgg ctcgttcttc tggagggtgc accccgagag tccggtgcgc
     4741 agccgccttc cgttgtccga ggacgagcga cgggtccccg aggacccata cagcctgagc
     4801 aaaggtgtca ccgaggagat cggtgaaacg ttccggcggg cctgggggat cgaggccgtg
     4861 aacctgcgct tcgctgcggt gtggtcggag gagaagtacg aggagctcct gacgaccggc
     4921 ctgccggtca cggaggcgtg ggccgacgac ctcttccagt gggttcaccc caacgatgtc
     4981 cgtgacggca tcatcctcgc ggcaactgtt gaccagacta cgagtgagcc catcgtgctc
     5041 gcggccgccg atacgcgggc acccgaggat acgctcgagc tcatccgccg attcaagccg
     5101 gagttggagc gggagttgcg tgagccgctt cccggccgca gttcactgct gtccatcacc
     5161 cgcgcacgat ccttcctcgg gttcgagccc cggcattcgt tcgctgccgc cgtgcagtcc
     5221 gacggacagc gctagcccgt cagggcaggc gcccgatggc tgattgaggc atggtggttg
     5281 cgacgattac tcttgatcga cgaagcacga tcggctccgc gtcccgatgg tcgaggagat
     5341 cgccgctggc gtctgagcaa ctgatcagga ggtcgtcgtg accgtgagtg ttcgggatgt
     5401 cgccgctgcc gcgggtgtct cggtgggcac ggtctcgaat gtgctcaacc gtcccgacat
     5461 cgtcgcgccc gcgacggtcg agcgcgtgca ggcggccatc gactcgctgg gcttcatccg
     5521 caacgatgcg gcacgtcagt tgcgggcggg caagagccgg accatcggca tggtcgtgct
     5581 gaacgtcggc aaccccttct tcaccgacgt cgctcgcggt gcggaggtgc gcgcgagcgc
     5641 ggccggtatg agcctgctcc tcggcaacag cgacgagaag tacgaccgcg agctggccta
     5701 ctttgacctg ttcgaggagc agcgggtgca tgggctcctc gtcaccccgg tgggcgacga
     5761 cctgagccgt ctggagcgaa tcagcaacaa cggcacaccg gtggtgctcg tcgaccgtga
     5821 ggccgatccc aacgccttct cgtccgtgtc ggtggacgac gtcagcggtg gattcctcgc
     5881 gacccgccac ctgctggaca cgggccggcg tcggctcgcc ttcgtcggtg ggcctgcctc
     5941 gatgcgtcag gtcgcggatc gtctcgcggg cgcccgtcgt gcggtctccg aggagtccgg
     6001 agccacgctg gagcacatcc cgaccgattc gatgaccgtc gagcacgggc gcgcagcggg
     6061 cgaatggatt cgtctgcgca gtcggtccga tcggccggag gccgtcttcg ccggcaacga
     6121 tctggtcgcg ttgggggtgc tccagggcct catgatgcac aacgacgtgc gcgtaccgga
     6181 ggacgtggcc ctcatcggct acgacgacat cgacttcgcc agcgccgcgg tcgtgccact
     6241 gtcatccatc aggcaaccgc gaatgctgat cgggcacacc ggagtggacc tcctgctgcg
     6301 gctggccgat gagggcgccg accacgagcg ggtcgtcttc cagccggagc tcgttgtcag
     6361 gtcgtcgaca gccgggtgag ccggtcgtcg tccggtcaca gatccagccg gatgaccctg
     6421 cccccgagtg cccactcggt gaggtagacc gcacccgtcg ggcccaccgc caacccgtgc
     6481 gggctgttga ggcatccctc gcggaactcg ggtgccacca cctcgccgtc gacgacccgg
     6541 ttgggccagc cctcccggtc gctcccgtgc gagtccgcaa cgatcacgtg cacacgatcg
     6601 gtgcggtcga tccgcagcag cgctccccgc aggtcggtca ccagcaggtc gtccccccgc
     6661 agcgccagtg aactcggggt gggcatcgcg tcatcagtga ccgccctgac gaactctcca
     6721 tcgagggtga ggaagatcac tcgctggttg ccgcgatcgg cgatggccac gagcggctcg
     6781 gggccgcggt agtcgatcgc gatgccgtgc gggcagtcga agcgcatccc cgtggtcgag
     6841 ccgtcgatgg tgcgggtgtc gcccgtcccg ctcacccggt gcaccaggct gcgaccgtat
     6901 ccgtccgcta tccagaggtc gccgcggtgg gccgtagccc ccgtcacgac cgccgtcgag
     6961 gtgggcttcc actgctccgc aaggccgtcg gtccccggtt ccgtcaggcg cgcaagcatc
     7021 gtgccgtcga ccctgatcct ggtgagctgc ccggactgcc ccaaggggcc ggggtcagcg
     7081 atccacagtt cgtttccgtc cgcaccctcg atcgcttcga tcccgtgcgc gtgtgcggag
     7141 ccggtccaca cgccgtcgat gtccccggtg tgctcgtcga gcagcaggag ctcgcctccg
     7201 ccgctcgccg agatcaccag ccgcccgtcg gcgagaacac cgatgcccga atgggaccac
     7261 cgatccgtcg gagttcccag cagcagggtc gtccagccga cggcgctcag ctccactcca
     7321 cgatcacgga gccggcgctg taggtggcca ccgggtgggc gaacggcagg gggttcagtt
     7381 gctcggcact cacgagcttc accgcgggca gagcggcgag gagttgcccg accgcctcac
     7441 gtatctcgat gcgcgcgatc tgctggccga ggcatccgtg gatgccgtgc ccgaacccca
     7501 ggtgcccgaa cgcatcccgc gtgatgtcga actcgtcggg attctcgaac cgctgcggat
     7561 cgcggttcgc tgccaccgct gagaccgaaa ccgactcacc ggcggcgaag cgggtgtcgc
     7621 cgagctcgac atcctcggta gcagttcgcg cgaacagggc gacgaacatc gtcccgtatc
     7681 gcatgaattc ctcgacggct cctcccatga gatccgggtc gtcgcgcagg gcggcgagct
     7741 ggtcgggatg ccgcagcaga gcgagcaggc tggtggccat catgtaggcg gcggcgtcgc
     7801 gaccggagat catgagcacg agggcgaccc agtcgacctc tcccgcgctg aggtcggatc
     7861 ggatgagatc gctcagcacg tcttcatcga gatcggagcg cttctgctcg atgacggccc
     7921 ttgcgaacag cacacgatcc tgcacgggcg cctcgcgcag aaagaggcgg gcgaactcgt
     7981 cagcacgggc gtcggggata cccaggatca ggcgatgcac ggcggccgag atcggcgctg
     8041 cgaattcggc catgaggtcg gcgggcgatc ccacggtgag gaggtgagcc acctggcgag
     8101 cgacgatggc ggcgatcgcg ggttgctgtg cacgtccggc gcgcacagag aagcgtgcca
     8161 tgaggatccg ccgcacgcgg ctatgcgcct caccatccag gaagagcagg ttcgctactc
     8221 tcttcgaggc cagcgcctcg tcgtcaaggt agggcagttc ggggccgccg gggatggtct
     8281 cacccgggat gcgttggggc tgctggctga accgcggatc ctccagcacc cgccgggcca
     8341 gctcgtggcg cgtgacgatc cagccgtcgt gcccgtcggt gtagtgcaac ggcgtgactg
     8401 ggccttcctc gcgccactca ccgagcacgg gggacggctc gagtggtgtg ccgtcggtgg
     8461 gcagcactcg cgggtgcacc gggcatccgg tcggggtcgt ggtcatcggt ggggtcctct
     8521 catcggtggg caggggcgag gtcgtcgaag cgtgcccgct cctgccggaa ctcgcgcggc
     8581 atgttccagc cgaccagtcc ggtcgggcgg ccatcgtgca gggtggcgcc gacgaagcgc
     8641 cggtcgtcca gggagccggc cagcagtgtg aggggcaggt cggcgttcag tcggccgtgg
     8701 accacgatgc gctgggagaa cagttccgtc cagtggaagg gcactatcgg ggggctcggt
     8761 tcgccggttg cgatggagcg cgccaccgcg agcgcctgct cgatagcggt ggcttgatgc
     8821 tccacccggc gagcgaggcc tgcgtcgggg tcgtaccacc gcgcgacgtc gcccacggcc
     8881 cagacgccgg gcgctgcgac tccgtgctca tcgcacagca ctccatcgct gatgtccaaa
     8941 tcggtaccga ccagcgcttc gacgcggggt gtgctgccta cagccgcgac gaccgtggtc
     9001 gcaggcacca ccgagccgtc ggcgagcatc accccggtga ccgagccggt ggtcgcaacg
     9061 ccgaccggct cgcaacccag ccgcaggtcg accccgttct cccgcagcac gctctccacg
     9121 agcgaagcga gatgctcccc ggtgcgtccc agcctcacat ggcggctgcg tcccagcacg
     9181 gccacgtcat gcccgagctg ccggatgcca gcggccagct cgagcccgag aactccagcc
     9241 ccgatcacgg cgaccggtcc cggacggtcg agttgagctc gcagactccg cgaatcctcc
     9301 acggtccgca cggtgtggat gctgccggcc gggtacgtgc cagccggtcg acgtgcactg
     9361 actcccgtcg cgatcacgag gtgctcgaag ggcagccagt cggcgccgac aagaactctc
     9421 cgcgcctgga gatcgacggt cgacgcgctg accccgcgca ggtaccggat gtccctgagc
     9481 tggaggtcgt cgccccggca gaggtggatg tcgtcttcgt tcagtcgtcc ggacagcagc
     9541 tgcttcgaca gcggaggtct gctgtacggc gcccggtcct cgtcgccgac gagtgtgact
     9601 gggtcttcgt agccatcgtc ccggaggttc agcgccgtgg tgaccccggc aacaccagcg
     9661 cccagtatca gggttccggc accggcccgg tcggtcactc cgactcgtcg acgacgatca
     9721 cccgggccgg gcagagggcc gcagcgcgcc gggtggcggc atgctcgggt tcggagggcg
     9781 cagcgttcag gagcatcaca atgccgtcgt catcctggtc gaacacatcc ggggccacca
     9841 gggcgcactg acctgcgccg atgcaggctg cggtgtcgac gttgactctc acgagtttcc
     9901 ttccctgcgc tcgccgacgg cgacgatcac cccacaacgg tacgagtcag acgagggatg
     9961 gtgccatgcc ccgatcgagc aaatcttgga cgattcgcgc agcgatcctt ctctctttcc
    10021 gccgattgct tgcacgggta gcctcgtgac atggatagga cggcggtgaa cccggtcgac
    10081 cagacgcccg aagccggtgg tcctgttgtc atcggcgcca actggtaccg cttccgggtg
//
LOCUS       DAOSOU010000060        10096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6665_length_10096_cov_5.757295, whole genome shotgun sequence.
ACCESSION   DAOSOU010000060 DAOSOU010000000
VERSION     DAOSOU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6665_length_10096_cov_5.757295"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PLW15_04915"
     CDS             complement(<1..1244)
                     /locus_tag="PLW15_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT33775.1"
                     /translation="MRTRRSRARISFVVVTIFAIVAAFTVRLVDIQLVQASELNAQSL
                     SKRAQEQVLYGTRGSILDTNGAVLAASVERYDIEVSPRSALARTDAATSVPEALGKIA
                     ALTGQDPAALMAILTADPQSDFAYLTKGVKLDVFEAIRALDIPWVYYELRPSRTYPNG
                     AVAGNLVGFIGTDGPQAGIEIGEDSCLASSDGSASYEKGEDGVQLPGSLVTEVAPKDG
                     GTVRLTIDRDLQFYVQQRLMQVYSELGAEWATAVVMRVSDGHLMAVADAPSVDPNNVD
                     GVPNTALGSRAFSWPYEPGSTIKAVTAASLLDAGVVSQTSEVVAPGRLYLSNGDYIKD
                     AWAHDDTPYTLAGALVDSSNTAVSLFSDLLPADKRHDYLTAFGFNSETEVGFYGESEG
                     VVHPANEWDEVTNYAVQFGQGM"
     gene            complement(1252..1878)
                     /locus_tag="PLW15_04920"
     CDS             complement(1252..1878)
                     /locus_tag="PLW15_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33776.1"
                     /translation="MSSNLAYARPTVRPAHRPAEAPVRSVRPVPRRTEVAATRDQRRA
                     RPKAAYAIITVASLFLIFAAQLLLSIVVSQGAYQIESLQDQRKDLVRTQEALTENLEL
                     YGSSQNLAANAAHLGMVPSAYPLFLDLETGAVSSAPGSVDRVGCGGSCDLVANSLLTG
                     VPLVGTTPPASTAPTTASTSSTATASAAPAPTPVVPATVDTLPAPVTH"
     gene            complement(1875..2828)
                     /gene="rsmH"
                     /locus_tag="PLW15_04925"
     CDS             complement(1875..2828)
                     /gene="rsmH"
                     /locus_tag="PLW15_04925"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT33777.1"
                     /translation="MADNSIHTPVLLERTLELLGPALEHEGAVLVDATLGMAGHTEAF
                     LERYPTLTVVGIDRDPDALAIARERLAPYGARVHLVHAVYDGILSVLSELGIDRVSGV
                     LFDLGVSSLQLDRVERGFSYSKDAPLDMRMDSTGERTAAAILATYSESELRRIFYEYG
                     DEKLAPRFASRIVQSRDATPLVSSAQLVELIAAATPAAAQRAGHPAKRVFQALRIEVN
                     QELSVLERAIPDAIDALEVGGRIVVLAYQSLEDRIVKRVLQARSASTAPRGLPVELPE
                     HRPELKLLVRGAELADDDEKAANPRATPVRLRAAERLRYVA"
     gene            complement(2927..3358)
                     /gene="mraZ"
                     /locus_tag="PLW15_04930"
     CDS             complement(2927..3358)
                     /gene="mraZ"
                     /locus_tag="PLW15_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT33778.1"
                     /translation="MFLGTHTPKLDEKGRVILPAKFREELASGMVVTRGQERCLYVFS
                     QREFEDITQKIRQASITSKQNRDFIRLFLSGASQEIPDKQNRITLPTNLRTYAGLDRD
                     LTVIGAGSRAEIWDTEAWNAYYESAEADFVNTTEEVIPGLF"
     gene            complement(3591..3968)
                     /locus_tag="PLW15_04935"
     CDS             complement(3591..3968)
                     /locus_tag="PLW15_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HOT33779.1"
                     /translation="MPLSEQEQRLLEEMERSLYQNDADFVATVNPRRGKPNYTVLAIG
                     VLIAVVGVVALLGGVIIHQPIVGVLGFVVMFVGVLLAVAPPRRRGSAADAAGMPSGRK
                     SGSSRFMDTLNERWDQRQDGGRD"
     gene            4042..5163
                     /locus_tag="PLW15_04940"
     CDS             4042..5163
                     /locus_tag="PLW15_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT33780.1"
                     /translation="MAESTRLVDLIQLRIDEFLSEQSGQLATIAEELTELTDIARGFL
                     AGGKRFRALFCYWGWQSAAGVTSGFDPLPTDEASADLDAVVMAATALELFHAAALVHD
                     DIMDNSDTRRGAPAAHRLFERHHIADGWTGNPTAFGESAALLLGDLLLGWSDELFDRG
                     TSMLADRAAGVAGRAEFIRMRTEVTAGQYLDILEENSWATYPESTALARAHRVIVYKS
                     AKYSVEAPLTIGAALGGASLAQLAALRSFGLPLGVAYQLRDDLLGVFGDPEVTGKPAG
                     DDLREGKRTVLIALARTRLPASSRNLMDELLGDPGLDSQQVDMIRTALRDCGAIEEVE
                     KIIEHNVGLAVAALKDAPIAPSARAQLLQLADTVTRRSA"
     gene            complement(5170..5511)
                     /locus_tag="PLW15_04945"
     CDS             complement(5170..5511)
                     /locus_tag="PLW15_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HOT33781.1"
                     /translation="MTESPEVRWLTIPDLTEILGLKVSQVRRLIEDRALLATRADGVW
                     KVPDLFIVDGEPMHELRGTLVVLADAGFSDEEAMNWLLNEEESLGVSPVRALLAGRKA
                     EVRRVAQALGF"
     gene            5703..7118
                     /locus_tag="PLW15_04950"
     CDS             5703..7118
                     /locus_tag="PLW15_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT33782.1"
                     /translation="MTDTPADDADTVARAVFAGLTPRVAVAEAQRETGRIARGAFATV
                     PIVLVGSMAAMTLNLTTTAEPANARPALKPRAATSELGRTIREAIAAANTAIKSTTDS
                     IGITAASAPAMYTVAGGDTVSDIAGRFGLSTASVLALNGLGWSSLIFPGQQLKLTASG
                     PVPPVAAPEEAPEIGRYTIVAGDTISAIADRFGVSTLSILTANGLGWSSIIYPGQTIA
                     IPGVIAPGAFDQIADASAPDPAAPAQPSPAPPVAAPIVETEPPAVEPAPAPVSDPEPS
                     GGNYTVVSGDTLSSIAARFGIDTQSLLDANGLTWESTIYPGGVLVIPGVSSPASVGGE
                     TTGLNDEMRANAAVIIQVGYDLGVPEYGIVIALATAMQESSMRNLSWGDLDSVGLFQQ
                     RPSAGWGSVEQLTDPAYAARLFYGGPNNPNRGYTRGLLDISGWQSMSLTEAAQRVQIS
                     AYPDAYAKWEASARAWFAELG"
     gene            7181..9127
                     /gene="pknB"
                     /locus_tag="PLW15_04955"
     CDS             7181..9127
                     /gene="pknB"
                     /locus_tag="PLW15_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOT33783.1"
                     /translation="MSVNSTDPMIGRLLDGRYQVRSRIARGGMATVYVATDLRLERRV
                     AIKIMHGHLADDSQFKERFIQEARSAARLAHPNVVNVFDQGQDSDMAYLVMEYLPGIT
                     LRDLLQEHKVLTTEQTLDIMEAVLAGLAAAHKAGIVHRDLKPENVLLADDGRIKIGDF
                     GLARAASANTATGAALLGTIAYLSPELVTRGIADTRSDIYAVGIMMYEMLVGEQPFKG
                     EQPMQIAYQHANDSVPAPSAKNPKVPAELDELVLWATERDPEQRPKDARVMLDHLFET
                     HSALMTALPTTASTSTQRTMVMPSAAASADTTAETQVIGSRQRPVAALAPADSTAQLA
                     TAAAKRKRRGWVLFAIIAVIALLAGGTGWYFGAGPGSRVTIPDSIAGMSIADATAALE
                     KLGLTVDQTTGTIDSPTVPVDQVANTDPPFGTGVAPGSTVQLLLSTGPKQLPVPAFAG
                     MSEADATAAVKAAPFTLKDTLHQFDATVAEGTVIDALAGDGSSLTGVATYGELQPVTL
                     IVSAGALPDLAGLSVDDATAKLTAVGLELGTINQAEFSDTVPQGNVIRAQGVDGATVI
                     RTGDTVDLITSKGVEQVEVPDVTGMTVNEATDTLVAAGFVVADPGLNDGLRNVYRVTG
                     TNPSAGQVVNKGSTVSFTGFSIGG"
     gene            complement(9129..>10096)
                     /locus_tag="PLW15_04960"
     CDS             complement(9129..>10096)
                     /locus_tag="PLW15_04960"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOT33784.1"
                     /translation="RGDVTLPAYRGDIVNGYDFTPESRRADPARLIEGYHTSASTLNL
                     VRAFTQGGFADLRQVHSWNKGFASNPANHRYEKVANEIDRAIKFMEAAGADFDELRRV
                     EFYSSHEGLLMDYERPMTRIDSRTGTPYNTSAHFLWIGERTRDLDGAHVDYFSRVRNP
                     IGVKLGPSTTAETMKQLIDKLDPEREPGRLTFITRMGAGKVRDALPPLLEAIKGMDAT
                     PLWVTDPMHGNGLTTPNGYKTRRFDDVVDEVKGFFEAHRAAGTYPGGIHIELTGDDVT
                     ECLGGSEQIDEATLATRYESLCDPRLNHMQSLELAFLVAEELTAF"
BASE COUNT         1749 a         3380 c         3305 g         1662 t
ORIGIN      
        1 ctcatgccct ggccgaactg caccgcgtag ttggtgacct cgtcccactc gttggccggg
       61 tgaacgacgc cctctgactc gccgtagaag cccacctcgg tctccgagtt gaagccgaag
      121 gcggtgaggt agtcgtgacg cttgtcggcc ggaagcagat cggagaacag cgagacggcc
      181 gtgttcgagg agtcaaccag ggcccccgcg agcgtgtagg gggtgtcgtc gtgagcccag
      241 gcatccttga tgtagtcgcc attcgacagg tacagccgac ccggcgcgac gacctcgctg
      301 gtctgggaga ccacgccggc gtcgagcagg gacgcggccg tgacggcctt gatcgtggat
      361 ccgggctcat acggccagct gaaggcccgc gagccgagtg cggtgttcgg cacgccgtcg
      421 acgttgttgg ggtcgacgga tggcgcatcg gcgaccgcca tcaggtgccc gtcgctgacc
      481 cgcatcacca cggcggtggc ccactctgcg cccagctcgg agtagacctg catgaggcgc
      541 tgctgcacat agaactgaag gtcgcgatcg atcgtcagcc gaacggttcc gccgtccttc
      601 ggcgcgacct cggtcacgag actgccgggc agctgaactc cgtcctcgcc cttctcgtac
      661 gacgctgacc cgtcggagct ggccagacag ctgtcctcac cgatctcgat tccggcctgc
      721 ggcccatccg taccgatgaa tcccacgagg tttccggcca cggcaccgtt ggggtaggtg
      781 cggctggggc gcagttcgta gtagacccac gggatgtcca gggcacggat cgcctcgaac
      841 acatcgagct tgacgccctt cgtgagatag gcgaagtccg actgaggatc tgcggtgagt
      901 atcgccatga gtgcagcagg atcctgcccg gtgagcgcgg cgatcttgcc gagcgcctcg
      961 ggcaccgacg tcgcggcgtc cgtccgggcg agtgcgctgc gcggcgacac ttcgatgtcg
     1021 tagcgctcga cgctcgccgc aagcactgcc ccattggtgt cgagaattga cccacgggtg
     1081 ccgtagagca cctgttcctg cgccctcttc gacagggact gagcattgag ttcgctggcc
     1141 tgcacgagct ggatgtccac gagccgcacg gtgaacgccg caacgattgc gaagatggtc
     1201 accaccacga aggagatgcg agccctgctg cgcctggttc tcatgggcgg cctagtgcgt
     1261 caccggcgcg ggaagggtgt ccacagtcgc cggcacgacc ggcgtgggtg ccggtgcagc
     1321 tgaggcggtc gcggtgctgg acgtgctcgc tgtggtgggc gctgtcgaag ccggaggcgt
     1381 cgttcccacc aacggaactc cggtgagcag cgaattggcg acgaggtcgc agctgcctcc
     1441 gcagccgacg cggtcgaccg agccgggggc gctcgagacc gcccccgttt cgagatccag
     1501 gaagagcggg taggcgctcg gcaccatacc cagatgcgcg gcattcgccg caagattctg
     1561 gctggatccg tacagttcga ggttctctgt gagagcctcc tgcgtgcgca cgagatcctt
     1621 gcgctggtcc tgcagactct cgatctggta tgcgccctgc gagacgacga tgctcaacag
     1681 cagctgcgcc gcgaagatca ggaacaggct cgcgaccgtg atgatcgcat acgcggcctt
     1741 gggccgagcc cgccgctgat cgcgagtggc tgccacctcg gtgcgccgcg gcacgggacg
     1801 caccgaacgg accggtgcct ccgccggacg gtgagccggc cgcaccgtgg gacgtgcgta
     1861 ggcaaggttg ctgctcatgc cacgtacctc agtctttcgg cggctctgag gcgcacggga
     1921 gtggctcggg ggttcgctgc cttttcgtcg tcgtcggcca gttcggcgcc acgtaccagc
     1981 aacttcaact cgggccggtg ctccggcagc tccacgggca ggccgcgggg ggcggtcgaa
     2041 gccgaacgcg cctgcagcac gcgcttgacg atccggtcct ccagcgactg gtaggcgaga
     2101 accacaatgc gtccgccgac ctcgagagcg tcgatcgcat ccgggatggc gcgctcgaga
     2161 accgacagct cctggttcac ctcgatgcgc agggcctgga agacccgctt ggccggatgt
     2221 cccgcccgct gtgccgcggc gggcgttgcg gcagcgatca gctcgaccaa ctgtgccgac
     2281 gacaccaatg gggtggcgtc gcgtgactgc acgatgcggc tggcgaaccg cggcgcgagc
     2341 ttctcgtccc cgtactcgta gaagatcctg cgcagttccg actcgctgta ggtcgcgaga
     2401 atcgccgcgg cagtgcgctc acccgtggaa tccatgcgca tgtcgagcgg cgcatccttg
     2461 gaatacgaga aaccgcgctc gacccggtcc agctgcagcg acgacacccc gaggtcgaag
     2521 agcacaccgg agacgcggtc gatcccgagt tcggagagca ccgacaggat gccgtcgtag
     2581 accgcgtgca cgagatgaac ccgggcgccg tagggcgcga ggcgctcgcg ggcgatggcg
     2641 agcgcatccg ggtcacggtc gatgccgacg acggtcagcg tggggtatcg ctcgagaaat
     2701 gcctcggtgt gaccggccat gcccagggtt gcgtcgacga gaaccgcgcc ctcgtgctcg
     2761 agcgcgggtc cgaggagctc gagagtgcgc tcgagcagca cgggagtgtg gatggagttg
     2821 tctgccatgg cgatatgggc cgttcccggg agctgatccc catccggtac gacctgacat
     2881 cggggaagaa agtcagggcc tgccggctgg gagtcagttc cagggatcag aagagtcccg
     2941 ggatcacctc ctccgtggtg ttcacgaagt cggcttccgc gctctcgtag taggcgttcc
     3001 acgcctcggt gtcccagatc tcggcgcgac tgcccgcacc gatcacggtg agatcgcggt
     3061 cgaggcccgc gtacgtgcgc aggttggtgg gaagagtgat gcggttctgc ttgtcgggaa
     3121 tctcctgact ggcgccggag aggaacaggc ggatgaagtc acggttctgc ttgctggtga
     3181 tcgaggcctg acggatcttc tgcgtgatgt cttcgaactc gcgctggctg aagacgtaga
     3241 ggcagcgctc ctgcccgcga gtgaccacca tgccggatgc cagctcctcg cggaacttcg
     3301 ccggaaggat gacgcgcccc ttctcgtcca gcttgggcgt gtgggtgccg aggaacacga
     3361 cagcctcccc tcacgctccg gcccggattc aggtatcccc cactttactc cactttgcac
     3421 cacttaacaa ggacattgag tggatgccgc cgattactcg tctagcaatg ccagcaattt
     3481 cgcggaattt gccagtggag ggaagtggag caagattccc tgttgcgcgc cgaaatgggc
     3541 acaaaaaaag ggccagcccg aaggctggcc ctgaaagtgc gggtggcggt ctagtcgcga
     3601 ccaccgtctt ggcgctgatc ccagcgctcg ttgagcgtgt ccatgaagcg agatgacccg
     3661 ctcttgcggc cggacggcat accggcggca tccgcggcac tgccgcgccg gcggggtggg
     3721 gccacagcaa gaagcacacc gacgaacatg acgacgaatc cgagaacgcc cacgatcggc
     3781 tggtggatga tcaccccgcc cagaagtgcc acaacaccga caacggcgat caggacaccg
     3841 atcgccagca ccgtgtagtt gggcttacct cgacgtggat taacggtcgc gacgaaatcg
     3901 gcgtcgttct ggtagaggct gcgctccatc tcttccagga ggcgctgctc ttgctcggaa
     3961 agtggcatcc tcttcccttc cggtggacac tgactcgcgg aatcccatag tacgcctcag
     4021 cgcggtggct aggctagacg ggtggctgag agtacccggt tagttgacct gattcaactg
     4081 aggatcgacg agttcctctc ggagcaatcg ggtcagctcg cgaccattgc cgaagagctc
     4141 acggaactga ccgacatcgc gcggggcttt ctcgctggcg gtaagcgatt ccgcgccctg
     4201 ttctgctact ggggctggca atctgcggcc ggtgtgacca gcggattcga cccgctaccc
     4261 actgacgagg catccgccga tctcgacgcc gtggtgatgg ccgccaccgc cctcgagctc
     4321 ttccacgccg ccgcgctggt gcacgacgac atcatggaca attccgacac ccgtcgcggc
     4381 gcgcccgcag cccatcgcct gttcgagcga caccacatcg cggacggctg gaccggcaac
     4441 ccgaccgcct tcggcgagtc cgccgccctc ctgctcggcg acctgctgct cgggtggagc
     4501 gacgaactgt tcgaccgcgg tacgagcatg ctcgccgacc gagcggccgg agtcgccggt
     4561 cgtgccgagt tcattcgcat gcgcaccgaa gtgacggcgg gccagtacct cgacatcctc
     4621 gaagagaatt cgtgggcgac ctatcccgag tcgacggccc tggcccgggc acatcgcgtg
     4681 atcgtctaca agtcagccaa gtacagcgtg gaggcgccgc tcacgatcgg cgcggccctc
     4741 ggcggagcga gcctggcgca gctcgcggcc cttcgctcgt tcggcctacc cctcggagtt
     4801 gcgtaccagc tcagggacga tctgctcggc gtcttcggag atcccgaggt caccggcaag
     4861 cccgcgggcg acgaccttcg cgagggcaag cgcactgtgc tcattgcgct cgcccgcacc
     4921 cggctgcccg cctccagtcg caatctcatg gacgagttgc tcggtgatcc gggactcgat
     4981 tcgcagcagg tcgacatgat cagaaccgcc ctcagggatt gcggagccat cgaggaggtc
     5041 gagaagatca tcgagcacaa cgtggggctg gccgtggccg ccctgaagga tgcgccgatt
     5101 gcgccatccg caagggctca gctgcttcag ctcgccgaca ccgtcactcg acgctcggcg
     5161 tagcccaggt cagaatccga gagcctgagc gacccgccgt acctcagcct tgcggccggc
     5221 aaggagggca cgcacgggac tcactcccag gctctcctcc tcattgagca gccagttcat
     5281 ggcctcctcg tctgagaagc ccgcatcggc caggacaacc agggtgccgc ggagctcgtg
     5341 catgggctcg ccgtccacga tgaacaggtc cggcaccttc cacaccccat cggcacgagt
     5401 ggccagcagt gcgcggtcct cgatgagtcg gcgcacttga ctcaccttga gccccagaat
     5461 ctccgtgaga tcgggaatgg tcagccagcg cacttcgggt gattcggtca ctctccaaga
     5521 ctgccacgga cggtgttgag agagagggga tgctcgcccg aaccaaatgt ttcgaatatg
     5581 taaattcaaa tcacacaagc aacggtaatt gacactcatc ttcttctgtg acaatttggt
     5641 tccatggcca tcggtatcga tgaccgcgcc ttctcggggc cggggagcag gacggaacag
     5701 caatgaccga cacaccagca gacgatgcag acacagtcgc acgagccgtc ttcgccggac
     5761 tcaccccgcg agtcgccgta gcggaggcgc agcgcgagac cgggcgcatc gcgcgtggcg
     5821 cattcgcgac cgttcccatc gtgctcgtgg gatccatggc ggcaatgacg ttgaacctca
     5881 cgacgactgc cgagcccgcc aacgcccgac cggcgctgaa gcctcgcgct gccacatccg
     5941 aactcggccg cacgatccgc gaggcgatcg ccgctgccaa cacggcaatc aagtcgacga
     6001 ccgactcgat cggcatcact gccgcttcgg cacctgcgat gtacaccgtc gccgggggcg
     6061 acaccgtcag tgacatcgcg ggtcgtttcg gtctttccac cgcctcggtg ctggccctca
     6121 acggactggg ctggtcgtcg ctgatctttc ccgggcaaca gctcaagctc accgccagcg
     6181 gacccgttcc gccggttgcc gcacccgagg aggccccgga gatcggccgg tacaccatcg
     6241 tcgcgggcga cacgatcagc gccatcgccg accgcttcgg cgtctcgaca ctctcgatcc
     6301 tcacggccaa cggtctgggc tggtcgagca tcatctaccc cggccagacc atcgccattc
     6361 ccggtgtgat cgcgcccggc gctttcgacc agatcgcgga tgcctcggca cccgatcccg
     6421 ccgcgcccgc gcagccctcc ccagcgccgc ccgtagcggc cccgatcgtc gagactgagc
     6481 caccggcggt ggagccggcg cccgcgccgg tctccgaccc ggaaccgtcc ggcggcaact
     6541 acacagtggt ctcgggagac acgctgtcgt cgatcgcggc ccgattcggc atcgacaccc
     6601 agtcccttct ggacgcgaac ggcctcacct gggagagcac gatctacccg ggcggcgtgc
     6661 tggtgatccc cggtgtgagt tcccccgcga gtgtcggcgg tgagaccacc ggcctgaacg
     6721 acgagatgcg cgccaacgcg gctgtgatca tccaggtcgg atacgacctc ggggtgccgg
     6781 aatacggaat cgtcatcgcc ctcgccacgg cgatgcagga atccagcatg cgcaacctca
     6841 gttggggcga cctcgactcg gtcgggctgt tccagcagcg tccgagcgca ggctggggtt
     6901 cggtggagca gctcaccgat cccgcgtacg cggcacgcct cttctacggc ggaccgaaca
     6961 accccaaccg tggctacacc cgcggacttc tcgacatctc gggatggcag tcgatgtcac
     7021 tcaccgaggc cgctcagcgg gtgcagatct ccgcctaccc ggacgcgtac gccaagtggg
     7081 aggcctccgc gcgcgcctgg ttcgccgaac tgggttgatc ggtggctcaa gcgccgtgtc
     7141 tagcgggagt cttacagcgg ttcaacttta gactcggacg gtgagcgtca atagcaccga
     7201 cccgatgatc ggacgtctcc tcgacggccg atatcaggtc cgctcacgga tcgcgcgggg
     7261 tggcatggcc accgtgtacg tcgcgaccga cctgcgcctc gagcgccgcg tcgcgatcaa
     7321 gatcatgcat ggccacctgg ccgacgacag ccagttcaag gaacgcttca ttcaagaagc
     7381 ccgatccgca gcgcgactgg cgcatccgaa tgtggtcaac gtcttcgacc agggccagga
     7441 cagcgacatg gcctacctgg tgatggagta cctgccgggc atcacgttgc gcgaccttct
     7501 gcaggagcac aaggtgctca cgaccgagca gaccctcgac atcatggagg cggtgctcgc
     7561 cggtctcgcg gccgcgcaca aggcgggcat agttcaccgc gacctgaagc cggagaacgt
     7621 gctgctcgcc gacgacggac gcatcaagat cggcgacttc gggctggcgc gcgcggcatc
     7681 cgccaacacg gccaccgggg cggcactgct gggcacgatc gcctatctct cccccgaact
     7741 cgtgacccgc ggcattgccg atacccgcag cgacatctac gcggtcggca tcatgatgta
     7801 cgagatgctc gtcggcgagc agccgttcaa gggtgagcag cccatgcaga tcgcctacca
     7861 gcacgccaac gactccgttc ccgcgccgag cgcgaagaac cccaaggtgc ccgcggaact
     7921 cgacgaactc gtgctctggg cgaccgaacg cgaccctgag cagcgtccca aggacgctcg
     7981 ggtcatgctc gaccatctct tcgagaccca cagcgctctc atgacagcgc tgccgacgac
     8041 ggcatccacg tcgacccagc gcaccatggt gatgcccagc gccgcggcat ccgccgacac
     8101 gactgccgag acgcaggtca tcggctcgcg gcaacgaccg gtagcagccc tggcgcccgc
     8161 agatagcacg gcacagctgg caacggccgc ggccaagcgc aaacgccgcg gctgggtgct
     8221 cttcgcgatc atcgccgtca tcgctcttct ggcgggcggc accggatggt acttcggcgc
     8281 cggtcccgga tcccgcgtca ccatccccga ctccatcgcg ggaatgtcga tcgcggacgc
     8341 cactgccgcg ctcgagaagc tcggcctcac cgtcgaccag accacgggca cgattgacag
     8401 cccgacggtc ccggtggatc aggttgccaa caccgatccg ccgttcggca cgggggtggc
     8461 tccggggtcg accgtgcagc tcctgctctc caccgggccc aagcagctcc cggtgcccgc
     8521 cttcgcgggg atgagcgagg cggatgccac ggccgcggtc aaggcggccc cgttcaccct
     8581 caaggacacc ctccaccagt tcgatgcgac ggtggccgag ggaactgtca tcgatgccct
     8641 cgccggagat ggatcaagcc tcacgggggt ggcaacctac ggcgagctgc agcccgtgac
     8701 gctgatcgtc tccgccggtg ccctgcccga cctggccggc ctgtcggtgg atgacgccac
     8761 ggccaagctc acggctgtgg gactggagct cggcaccatc aaccaggcgg agttcagcga
     8821 caccgttccg cagggcaacg tgatccgcgc acagggggtg gacggggcca cggtcatccg
     8881 tacgggcgac accgtcgatc tgatcacgtc gaagggcgtc gagcaggtcg aggttccgga
     8941 tgtgaccgga atgaccgtca atgaggcaac cgacactctc gtcgctgccg gcttcgtcgt
     9001 tgccgaccct gggctcaacg acggcctgcg caacgtctac cgcgtcaccg gaaccaatcc
     9061 atccgccggc caggtcgtga acaaggggtc gacggtgagc ttcaccggct tctccatcgg
     9121 cggctgactc agaaggccgt gagctcctcg gccacgagga aggcgagttc gagcgactgc
     9181 atgtggttga gccgcgggtc acagagcgac tcgtagcgcg tcgcgagagt ggcctcgtcg
     9241 atctgctcgg atccgcccag gcactccgtg acgtcatcac cggtgagctc gatgtggatg
     9301 ccgccggggt acgtgcccgc cgcccggtgc gcctcgaaga agcccttgac ctcgtcgacg
     9361 acatcatcga accggcgagt cttgtagccg ttgggggtgg tcagtccgtt gccgtgcatc
     9421 gggtcggtga cccagagcgg cgtggcatcc atgcccttga tggcttccag aagcggcggg
     9481 agcgcgtcgc gcaccttgcc tgcacccatg cgcgtgatga acgtgagacg accgggctcg
     9541 cgctcggggt cgagcttgtc gatgagctgc ttcatcgtct ccgcggtcgt cgagggcccc
     9601 agcttgacgc cgatcgggtt gcgcacgcgg gagaagtagt cgacatgagc gccgtcgaga
     9661 tcgcgggtgc gctcaccgat ccagagaaag tgcgcgctcg tgttgtacgg cgtgcccgtg
     9721 cgggagtcga tacgggtcat cgggcgctcg tagtccatga gcaggccctc gtggctcgag
     9781 tagaactcga cgcggcgcag ctcatcgaag tccgcgccgg cagcctccat gaacttgatg
     9841 gcacggtcga tctcgttggc gaccttctcg tagcggtggt tcgccgggtt cgaggcgaac
     9901 cccttgttcc acgagtgcac ctggcgcagg tcggcgaagc caccctgcgt gaacgcgcgc
     9961 accagattca gggtcgacgc cgaggtgtgg tagccctcaa tcaggcgtgc gggatcagcg
    10021 cggcgggact cgggcgtgaa gtcgtagccg ttgacgatgt caccgcggta cgcgggaagc
    10081 gtgacgtcgc cgcggg
//
LOCUS       DAOSOU010000061         9870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6848_length_9870_cov_7.057259, whole genome shotgun sequence.
ACCESSION   DAOSOU010000061 DAOSOU010000000
VERSION     DAOSOU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6848_length_9870_cov_7.057259"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="PLW15_04965"
     CDS             complement(<1..1775)
                     /locus_tag="PLW15_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT33785.1"
                     /translation="MTPQRFSVPDVIYAVGMCVTVLVAFRSRTTVLGPFERYAFGGLL
                     VVGLVHRMSVLVENLSLTVGLEARVRARTSDLEKRERYFAALVRSSSDIVIVVDEART
                     IRSVSASLDDIYGWDHHRLVGRPLDSFGPFEALIAAVDAAAANSGRVHRVEWQLVDAS
                     GVTRSAESTVSNLLGDADIGCFVINTRDVTDRATLETQLRHQAFHDPLTGLPNRALFA
                     DRVGHALRRSGVSGARVGLAMVDLDGFKAINDSHGHHAGDRVLRSVADQLTSALEPGE
                     TVARLGGDEFAILVEDIDDTVDPASIAERLLECIHVVVDVEGMQYEVRASVGLAISTR
                     STSHPADAVQRLLRDADIAMYSAKRAGRGAARVFRPEMYADAQDTFELQSQLRGAVER
                     GEMVLFYQPTFELATGRISGFEALIRWQHPTKGLIGPSKFIPLAEESGVIVSLGDWAL
                     RTAVGQLAAWSREFGDGGSLSMAINVSVGQLRSPGLLDSLREVIGDAGIDPRRIVLEI
                     TESMLISDTDTVVGLLHDMADLGVRIAIDDFGTGYASLANLQNLPLDVLKIDRTFVSG
                     EFERDGAPLLSTILNIGSTLGLMTVG"
     gene            complement(2100..3086)
                     /locus_tag="PLW15_04970"
     CDS             complement(2100..3086)
                     /locus_tag="PLW15_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HOT33786.1"
                     /translation="MAHATKQHPNAQLTPKGGRRMADLVIEGGWTIEASADRFQADPK
                     TVRKWRDRFLAEGEQGLLDRSSRPRRSPNRTPLRIRQMVIRLRKKRRWGAEHIAYEVG
                     VAPSTVQHILREAGLARLDRGDRATAGPVRRYQRDRPGELIHVDVKKLSSIPDGGGWR
                     TRGRGYDNEGSVTRRGGYRFLHTALDDRTRIVYSEILDDERGITAVGFWHRAVAWFAS
                     IGIRCERVLSDNGSCYRSGLWHRACAETGPTVKKTRPRRPQTNGKVERYHRILLEEWA
                     YIRPWNSERERHAGYDGFVHFYNHHRAHGALGWSTPMDTLTSLRDNVPGHHS"
     gene            3469..4689
                     /locus_tag="PLW15_04975"
     CDS             3469..4689
                     /locus_tag="PLW15_04975"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOT33787.1"
                     /translation="MAPAVTEIDVFVGIDMAKQDHYAQAISRDGTELFKRPVLNDETT
                     IVRLIDDARAHGSRVAMVVDQPASGAQLLLSLAAAHDVPVAYVTGLQMRRAADLYAGS
                     AKTDPIDAWVLADFARRNADRLTWLQVTDDLLVELRILNGRDVDLATDATRTINRCRD
                     AMTAISPALERAIGSRLGHAGIRDALAKWSTPTALRAAGQAKIRTRIAKRSPRLAAKA
                     TDEIWAALNAQSVTLPGEEAWGRVIADLCSDLDRIIGRREQLATEIETVFMSHPLGMV
                     LVTLCGFGPRTGARTLAEIGDPQRFADGGRLAAYAGLAPVDRRSGRSINSASQSRAGN
                     HRLKNAMFLAAFVAAQHDPAAKAYYERKRAEGKKHNAAVICLARKRCDIILAMLKNAT
                     PYQQPAHENLPQAA"
     gene            4715..5029
                     /locus_tag="PLW15_04980"
     CDS             4715..5029
                     /locus_tag="PLW15_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HOT33788.1"
                     /translation="MFTTACADCHSNQTKILWFEHAAPVEWYVASHVKDGRHALNIDT
                     WHTYAGEGADESAKVIRKGSMPPSFYTYLGLHGDAKLTPAERQELHDGLAKTLAADPP
                     KQ"
     gene            5066..5302
                     /locus_tag="PLW15_04985"
     CDS             5066..5302
                     /locus_tag="PLW15_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33789.1"
                     /translation="MTAWWNAQSAERRRVFAVTGIAFALIAARGLAIARLVDDVFGRD
                     GSTRLDPKITDFLVDHRTPGLTALARAVTHLPIH"
     gene            complement(6057..6494)
                     /locus_tag="PLW15_04990"
     CDS             complement(6057..6494)
                     /locus_tag="PLW15_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33790.1"
                     /translation="MEFLRKKLGSVAVGGALLLGGAAGAYALTGNAGAQDTTTTSAAA
                     SSQDTTSAADDGTTTDPGGPRDGAMGPHTANGITETVLTGDDLAKATSAAEAAVSGGT
                     VERAETDAEGAAFEVHMTKADGSHVTVKLDADFKVTSTEDGMR"
     gene            7589..8092
                     /locus_tag="PLW15_04995"
     CDS             7589..8092
                     /locus_tag="PLW15_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33791.1"
                     /translation="MATTAALLTIGLVGLASPASAAPHMNYSSSGVFWSGGGQGATWA
                     HQYITWSQNDGGIHRVHFKLGGYSHTCQGAYCLTDLNLPANQIQLVTLETVMWNGTTW
                     AQYAVNWPNPINNGASTSVASYLVADGIQPFPAWYYTRSTAKAFLSGAWRSYTSGAEQ
                     PAVNINY"
     gene            8252..8590
                     /locus_tag="PLW15_05000"
     CDS             8252..8590
                     /locus_tag="PLW15_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33792.1"
                     /translation="MSAFLAKAQADMVDACNKVLAAAPNDGPPPTLKSIEQNGFVPVI
                     GPTCDAGYAVSSPANNAGSSAAAGLEIREKPDMNSAVIGYMIPGNPGWVSIAQITEAG
                     LTVDEILAAR"
     gene            9431..>9870
                     /locus_tag="PLW15_05005"
     CDS             9431..>9870
                     /locus_tag="PLW15_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT33793.1"
                     /translation="MMNQENYVNVNDLHKQGWTISEIAAETGWHRTTVSKYLKDGPPA
                     ARATEAPVMTEEWRSRIDTMLGAYPRLLSVSVHNKLRAEGFEGSYPTVVRAVRDLRGP
                     RFRAANLVSVPIHTAPGDEAQFDFCDVSGWAARFGWRGPLFVFG"
BASE COUNT         1709 a         3290 c         3193 g         1678 t
ORIGIN      
        1 cccacggtca tgagcccgag ggtgctgccg atgttcagga tggtgctcag cagcggtgcg
       61 ccgtcacgtt cgaactcgcc actcacgaac gtccggtcga tcttcagcac gtcgagcggc
      121 aggttctgca ggttggcgag cgacgcatag ccggtcccga agtcgtcgat ggcgatccgc
      181 accccgagat cggccatgtc gtggagcagc ccgacgaccg tgtcggtgtc gctgatcaac
      241 atgctctcgg tgatctcgag gacgatgcgt cgcggatcga tgccggcgtc gccgatcacc
      301 tcccgcaggc tgtcgagcag cccgggcgac cgcagctggc cgaccgacac gttgatcgcc
      361 atcgacaggg acccgccgtc gccgaactcc cgactccacg ccgcgagttg gccgacggcg
      421 gtgcgcagcg cccagtcgcc cagcgacacg atcacgccgc tctcctcggc gagcgggatg
      481 aacttcgacg gtccgatcag tcccttcgtc gggtgctgcc accggatgag cgcctcgaac
      541 cccgagatcc gccccgtcgc gagttcgaat gtcggctggt agaagagcac catctcgcca
      601 cgttcgaccg cgcctcgcag ttgggactgc agctcgaacg tgtcctgggc atcggcgtac
      661 atctcgggcc ggaacacgcg tgctgcaccg cggccggcac gcttggccga gtacatcgcg
      721 atgtcggcgt cgcgaaggag gcgctgcacg gcgtcggcgg ggtgcgacgt cgagcgcgtg
      781 ctgatggcga gcccgaccga cgcccgcacc tcgtactgca tgccttcgac gtccacgacg
      841 acgtggatgc actcgaggag tcgttccgcg atcgacgccg ggtcgaccgt gtcgtcgatg
      901 tcctcgacga ggatcgcgaa ctcgtcccca ccgaggcggg cgacggtctc gccgggttcg
      961 agagcgctgg tcaactgatc ggcgacggac cggagcaccc ggtcgccagc atggtggcca
     1021 tggctgtcgt tgatcgcctt gaagccgtcg aggtcgacca tggcgagtcc gacgcgggcg
     1081 cccgagacac ccgagcgacg caatgcgtga ccgacacgat cggcgaagag cgcccggttc
     1141 gggagcccgg tcagcggatc gtggaacgcc tggtggcgca gctgggtctc gagcgtcgcc
     1201 cgatcggtga cgtcgcgggt gttgatcacg aagcacccga tgtcggcgtc gccgagcagg
     1261 ttcgacacgg tggattcggc cgatcgggtg acgccggacg cgtcgacgag ttgccactcg
     1321 acccggtgca cccgtccgga gttcgccgcg gcggcgtcaa ccgccgcgat cagcgcctcg
     1381 aacggtccga acgaatcgag cggccgaccg acgagccggt ggtggtccca tccgtagatg
     1441 tcgtcgagcg aggcactgac cgagcggatc gtccgcgcct cgtcgaccac gatcacgatg
     1501 tcggaggacg agcggaccag cgccgcgaag tagcgctcgc gcttctcgag gtcgctcgtg
     1561 cgggcacgga cccgggcctc caggccaacc gtgagcgaca ggttctcgac gagcaccgac
     1621 atccggtgca cgagcccaac gacgagcaac ccaccgaagg cgtaccgctc gaacggtccg
     1681 agcaccgtcg tgcggctccg gaaggccacg agcaccgtga cgcacatccc cacggcgtag
     1741 atgacgtcgg ggaccgagaa gcgctgtggc gtcatcgtta cccacggtgc gttcgaatgg
     1801 catcaggccg tatcgctcag catctcaggt tcgcccagaa gcgtcgcgac agcggcctgg
     1861 cctcggaacc accgggacca gcgtcatgtt cctcgtcatc atcgtcggcc tcgtgggctg
     1921 ggtcaccgtc gaccagcggc gccggtcatc cgagctgagc gcggcatgac cggactgccg
     1981 aacgaaccag cgggatcgag ccctcgaccg aagcgacgac gcgtcgttca gagctcgtcg
     2041 tcgcgctcgt gctcgtcgct gtgggcatcc agttcgttca ttccctacgg tgtcgacaac
     2101 tagctgtgat gaccggggac gttgtcccga aggctggtga gggtgtccat cggggttgac
     2161 catccgagcg ctccgtgggc ccggtggtga ttgtagaagt ggacgaagcc gtcgtagccg
     2221 gcgtggcgct cgcgctctga gttccatggc cggatgtagg cccattcctc gagcaggatc
     2281 cggtggtagc gctcgacctt gccgttcgtt tggggccggc gagggcgggt cttcttcacc
     2341 gtgggcccgg tctcggcaca ggcccggtgc cacagccccg acctgtagca cgatccgttg
     2401 tcggagagga cccgttcaca cctgatcccg atcgacgcga accaggcgac ggcgcgatgc
     2461 cagaacccga ctgcggtgat gccccgttcg tcgtcgagga tctcggagta cacgatcctg
     2521 gtccggtcat cgagtgccgt gtgcaagaac cggtagccgc cacggcgggt gacgctgccc
     2581 tcgttgtcat agccacgacc gcgggttcgc cagccgccac catcggggat cgaggacaac
     2641 ttcttaacgt cgacatggat cagctcgccg ggccggtcgc gttgatagcg gcgcaccggc
     2701 cctgcggtgg cccggtctcc gcgatcgagg cgagcaaggc cggcctcgcg caagatgtgt
     2761 tgcaccgtcg acggcgcgac cccgacctcg tacgcaatgt gttcagcgcc ccaacgccgc
     2821 ttcttccgca accggatcac catctggcga atccgcagag gtgtccggtt cggtgaacgc
     2881 ctcggccgcg atgaccgatc cagcagacct tgctcgccct cggcgaggaa ccggtcgcgc
     2941 catttccgga cagtcttcgg gtcggcctgg aaccggtcag cgctcgcctc gatggtccag
     3001 ccgccctcga tgacgagatc ggccattcgg cggccccctt tcggcgtcaa ctgcgcgttc
     3061 gggtgctgct ttgtagcgtg agccatgagt cctccgggtg agctgtgagt tgtggaagac
     3121 ccacatcctc tcccggaggc tcaactcacg atcggttcac ccacaccatc gccccaggga
     3181 caccgtcccc ggtcaacaca actagcccgc gacgagccga aatggggcag agtgcccgac
     3241 accttacctt ccttgtaggc cgtgagcaag actctcggct tggattgctc gacagcacgg
     3301 gcagggcttt ctgcccctgt cacacagatg gcctgggggg tgttgccacc cgtgctgttg
     3361 ggcgcggttg acctctgaca gggacatgcc ctcgggctga ttcgtaaggt ggttgacggc
     3421 gacctcgccc gcgagctccc tgcgaaccaa ccagctccag gaggtgacat ggccccagcg
     3481 gtgaccgaga tcgacgtgtt cgtcgggatc gacatggcca agcaagacca ctacgcccaa
     3541 gcgatcagtc gtgatggcac cgaactgttc aaacgccccg tcctcaacga cgagaccacg
     3601 atcgtccgac tcatcgacga cgcccgcgcc cacggatcgc gtgtggcgat ggtggtggac
     3661 cagccggcgt cgggcgccca actgctgttg agtctcgccg ccgcccacga cgtcccggtc
     3721 gcgtatgtga ccggactgca gatgcgccgc gccgccgatc tctacgccgg ttccgcgaag
     3781 accgacccga tcgacgcgtg ggtcctcgcc gacttcgcac gccgcaacgc ggatcggctg
     3841 acgtggctcc aggtcactga cgacctgttg gtcgagctgc ggatcctcaa cggccgcgac
     3901 gtggacctgg cgaccgacgc gacacgcacg atcaaccgtt gccgcgacgc tatgacagcg
     3961 atctctcctg cgttggaacg ggcgatcggg tcgaggctcg gccacgccgg catccgtgac
     4021 gcgctggcga aatggtcgac cccgacggcg ttgcgcgccg ccgggcaagc caagatccgc
     4081 acccggatcg cgaagcggtc cccgaggctg gcagcgaaag ccaccgacga gatctgggcg
     4141 gcgctcaacg cccagagcgt cacgctgccg ggcgaggaag cgtgggggcg tgtgatcgct
     4201 gatctgtgct cggacctcga ccggatcatc ggacgtcgag aacagctcgc taccgagatc
     4261 gagacggtgt tcatgtcgca ccctctcggc atggtcctgg tgaccctgtg cgggttcggg
     4321 cccaggaccg gagcacgaac cctcgccgag atcggtgacc cgcaacgctt cgccgatggc
     4381 ggccgcctcg ccgcgtacgc cggcctcgca cccgtcgacc gacgctcggg gcgcagcatc
     4441 aactcggcat cgcaatcgcg ggccgggaac caccgtctca agaacgccat gttcctcgca
     4501 gcgttcgtcg ccgcacagca cgaccccgcc gccaaggcgt actacgaacg caaacgcgct
     4561 gagggaaaga agcacaacgc tgcggtcatc tgcctcgccc gtaaacgctg cgacatcatc
     4621 ctcgccatgc tcaagaacgc gaccccctac cagcaaccgg cccacgaaaa cctcccccaa
     4681 gccgcttgac aacaagacag ggacaccccc ccagctgttc acgaccgcgt gcgctgactg
     4741 ccacagcaat cagaccaaga tcctgtggtt tgagcacgca gcgccggtcg agtggtacgt
     4801 cgccagccac gtcaaggacg gacgacacgc cctcaacatc gacacctggc acacctacgc
     4861 cggagagggc gccgacgagt cagcgaaggt catccgcaag ggctcgatgc ctccgagctt
     4921 ctacacttac ctcggacttc acggggatgc gaagctcacg cctgcggagc ggcaggagtt
     4981 gcacgatggt ctcgccaaga cgctcgccgc cgaccctccc aagcagtagc gccgtgccgc
     5041 caccgcccgg tgacatcatc gaaccgtgac agcgtggtgg aacgctcaat ctgctgaacg
     5101 ccggcgggtg ttcgccgtga ccggaattgc gttcgcgctg atcgccgcga ggggtctggc
     5161 aatcgcgagg ctggtcgacg atgtcttcgg cagggacggt tccacgcgtc tcgacccgaa
     5221 gatcaccgac ttcctcgtcg accatcgaac ccccggactc acggcgctcg cccgcgcggt
     5281 gacccacctg ccgattcact gacggtgacc gtcgtcgccg ttctgtccat cgcgatgctg
     5341 ctcgctcggg gccgccgacg actggcggca ttcgtacccc tgtccatggt cgacgctgca
     5401 gcagcgacat cgctcggcaa gctcgtcgtc gcccgccccc ggaccacccg aagcgatctg
     5461 gctcacccga gcatcgagcg cagccttccc atcgggagac gccacacact ccgtggcctg
     5521 ctggtgcgcg ttggcgatcc gcgctgccat gaccacccgc cgacggccgt tccaagctgc
     5581 ctggatcgct ctcggcatca cgctcgccgc ctccatcggc acatcacgtc tgtacctcgg
     5641 cgttcatcgc gcgtccgacg tcgtctgggg ctgagcagtc gcgacagcat ggctgctcgc
     5701 gctcgtggtg accggttggt cccgaccacg tctcgctacc gagatcaacg cagcgcggac
     5761 gcggctcgct caaggcgtcg ccgccgccag atcaacgaac ccccggtggc cgactgaggc
     5821 gacgctggtc gcgacaacct cgccgcccgc tgacttcgaa ccagcagatt ccgcatccgt
     5881 cgcaccaaat ctgagcgccg cctgatcggc gcgcttcaca taatcatagc tcggcaatcg
     5941 ctgggcgacc gctccgcacg aaccgccggg atacttcgga tcggaggcgc ggggcggcac
     6001 cgggaccagc gcggtcccga tgccgttcgt ggggaattcg ggcagttcga ggaggtctac
     6061 ctcatgccgt cctcggtgct cgtgaccttg aagtcggcgt cgagcttgac cgtcacgtga
     6121 cttccgtccg ccttggtcat gtgcacctcg aaggccgcac cctcggcatc ggtctcggcc
     6181 cgctcgacgg tgccacccga gaccgcggcc tcagcagccg aggtggcctt cgcgaggtcg
     6241 tcgccggtca gcaccgtctc ggtgatgccg ttggcggtgt gcgggcccat tgcaccgtcc
     6301 cggggtccgc caggatcggt tgtcgtgccg tcgtcggcgg ccgacgtcgt gtcctggctc
     6361 gatgcggctg cactggtcgt tgtggtgtcc tgagcaccgg cgttgccggt cagcgcgtat
     6421 gcaccagccg ctccgccgag gaggagggcc ccgccgacgg ccacgctgcc gagtttcttt
     6481 cgaaggaatt ccatgtctgt ctccgtttca ttttccctaa tgggagtcgc ccggaggatc
     6541 cggactggag acatcatcgg aactcgacgt gaggaggctg ggagacgggc gtgagaagtc
     6601 tgtaagactg ctgccgcctc cagtgactgg caggtcgcgt gggttcggtt ccgtcacccc
     6661 agaagggaac gacctcattc ttggtggctg ctgctcggta tccggatgag gaacacggct
     6721 ccgcctgctt cgcggtcggc gacctccact gatccgccga tctcggtcgc caggtcgcgg
     6781 acgatggcaa ggcccaagcc ggccccaccg tctctgcatc cggcgtccag tcgtcggcca
     6841 gctctccgcc ccgatctgcc cttgcggacg gcccgaacgg gcgaagcggt tgggctgctc
     6901 cgtgtcggcc gggtcgggca ccgaaacgtt tctcgggagt ctgtgccact tcttgaccac
     6961 ggtcgtggag cgcgagtcgt gggcgaggag agcggatgaa gaatgcggcc aaggtagaga
     7021 ctgaccggcg catcgcctgg gcgccatcag ccaccgaaat gacgcgcgaa ggttcagaag
     7081 ccgcaagcga ctccgaacgg cgagaccccg cccaggttgt tggaggcggg gtctcagggc
     7141 agaaggcgcc cgctcagtcc ggaggtgccg ccaccatgtg caatgctacc gctcgtcggc
     7201 accaccacca ccattgacgt ggcgatgacc gccgttacgt gccggcttca tcggcatgag
     7261 ccgaccaacc gtcattcctt tccagagcta ggtacctgca ggtcaacgct cagatcgacg
     7321 ccgatcgcag agagtttggg ggccgtgcag gacgacggcc gttagatggt cagggatgac
     7381 caacgccatg gtcagggctc gcaacgacgc tcatcggagg ggatggctgc agggtcggtc
     7441 gacatcgtga ccgaacctgc agccatccct acgtagtgct attatgttgg atctgacgac
     7501 tgaaggagtg cgaatgcccc acctcgacta cctctggcga accaaagaac cgctactgcg
     7561 tcttcgccgg gcgcgaaccc gtcgcaggat ggccaccact gccgcgctgc tgacgatcgg
     7621 gctcgttggg ctcgcgtcgc cggcgtctgc cgccccgcac atgaactatt cgagttctgg
     7681 cgttttctgg tctgggggtg ggcagggcgc cacctgggcc catcagtaca tcacgtggag
     7741 tcagaacgac ggcggtattc atcgggtgca cttcaagctc ggcgggtatt cccacacgtg
     7801 tcagggagcg tattgcctga ctgacctcaa tctgcctgcc aaccagatac aactggttac
     7861 tcttgagacg gtgatgtgga acggtacgac gtgggcacag tacgcggtca actggccaaa
     7921 tccgatcaac aacggcgctt ccacttcagt ggcgtcatac ctcgtggcgg atggaatcca
     7981 gccgttccca gcttggtact acacgcggtc cactgcgaag gcgttcctaa gcggagcttg
     8041 gcgttcgtac acgagtggcg cagagcaacc tgcggtcaac atcaactact gaagggtgga
     8101 tacaatgaac gcatttcgga gaatctcgac cgtacttgtt gcatctgtcg cgctagcggg
     8161 atgcggggcc gaggccgacg tcgctgtgca aggcgcgccc agctcgcccc aagcagcctc
     8221 acccgccggg ctgagcaagc catcggacac tgtgtccgcg tttctagcga aggcacaagc
     8281 tgacatggtc gacgcatgca ataaagtcct tgcggcggcg cccaacgatg ggccgccccc
     8341 gacactcaag tctattgagc agaatggatt cgttcctgtc attgggccca cctgcgacgc
     8401 gggttacgct gtgagctctc cggccaacaa cgcaggctcg tcagcagcgg ccggactcga
     8461 aatccgtgag aagcctgaca tgaacagcgc ggtgattggc tacatgattc cgggtaaccc
     8521 tggttgggtg tcgatcgctc agatcacaga agcgggcctc acggtcgacg agatcctcgc
     8581 cgcacgctag cggcggaggt cagctcacag cctgatccgg caaatctgtg ccggatcagg
     8641 ctttcgcaaa gctcgacgca gttgtgaatc gtcgccgcgg tctatcggca tcctcgatgg
     8701 cctcgacctc gaacgttggc gtcgcactcg acttgcgcgc tcgagactta cgcgaaggcc
     8761 gctgcgggcg agaagcgttc ggtgcagaac gaccagcgac tctcaccatc ctgccggccg
     8821 gaactcaagc cccgttcacg cagacgctgc caccatggtc ctgatccggt ttcgagaggg
     8881 cggggtccga ccccctagct ggcaggcgta caggcggaga ctgaggctgg cgttgattac
     8941 cgaccatctt gctgacgtga gggaggtcgg atgtcgcgcg ctgctacgga ccggccatca
     9001 cgctggtcgc acgccttccg ttgtgcgtgt atcgataacg cgggtgggtt gcgaatccga
     9061 tcaatcgaga cgcgcaaccc cagcagccgg ggccgtaccg gcaccagctg atcaatgtcg
     9121 atgacggtcg cgacgagtcc ggtgcgcgtc ggatcccctt cccgcacccc agaagattca
     9181 cgcacaacct ttcgccagct tgttccgctt gcggaacaag ccagtctgtg ccacttcctg
     9241 accacggtcc cagagcgcgc gaatcggggg gggtggcgca ggctgtgagc gatcactgag
     9301 atcttacttt cacgatcatt gaaaaccgac atgggtgccg ggttcctggc tggcatccac
     9361 caccggttgg ggtggcccct ccagcgtgtc ttgcgaccaa gcacgacagc gactggagga
     9421 gccgctctcc atgatgaacc aggagaacta cgtgaatgtg aatgacctgc acaagcaggg
     9481 ttggacgatc agcgagatcg ccgccgagac gggctggcat cggaccacgg tcagcaagta
     9541 tctgaaggac gggccgccgg cggcgagggc gaccgaagcg ccggtgatga ctgaagagtg
     9601 gcgctcgagg atcgacacga tgctgggggc gtacccgcgg ttgttgtcgg tgtcggtgca
     9661 caacaagttg cgtgctgagg ggttcgaggg cagctatccg acggtcgtgc gggcggtgcg
     9721 cgatctgcgg ggccctcggt tccgggcggc gaacttggtg tcggtgccga tccacacggc
     9781 accgggtgac gaagcccagt tcgatttctg tgacgtgtcg ggctgggctg cccggttcgg
     9841 gtggcgtgga ccactgttcg tgttcgggat
//
LOCUS       DAOSOU010000062         9862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6852_length_9862_cov_5.867034, whole genome shotgun sequence.
ACCESSION   DAOSOU010000062 DAOSOU010000000
VERSION     DAOSOU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6852_length_9862_cov_5.867034"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1159)
                     /locus_tag="PLW15_05010"
                     /pseudo
     CDS             complement(114..1159)
                     /locus_tag="PLW15_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1159..2688)
                     /locus_tag="PLW15_05015"
     CDS             complement(1159..2688)
                     /locus_tag="PLW15_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33794.1"
                     /translation="MATFIVRRLIASIFIFLAATYLMYILTALSGDPLEDLRTSTAPN
                     KQQLIEARTALLHLDVPPYLRYFIWIAGVGKCFIGQCDLGVTVQNQPVTVLLAQAAGS
                     TLQLVTIASVIAIILGLVVGITTALRQYSGYDYGVTFLSFLFFSLPIFWVAVLLKQFV
                     AIGFNDFLSDPQIAPWIIPILAVISGLIWMSILPGKGRRKLITFAAAALASAAVIIYM
                     DLSDWFSYPHIGIVGVALTLVGAAFGVTTISTGLKNRRALYASLVVAGIGVAAWYPFI
                     YFTTGLPFWGWIGIGVATVLVALAVGWFMGGDDRRPVARTAAIVGFVAFSVLSLDRFM
                     QVWDDYANSGRIKGRPIATIGSGTPGLEGSLWVHGVDLFTHLLLPTLALILISFATYT
                     RYSRASLLEVMNQDYIRTARAKGLTERTVVVRHAFRNALIPIATIVAFDIGGIIGGAV
                     ITERVFGWTGMGALFQNGLDHVDPNPVMAFFLVTGSLAILFNLIADLVYAGLDPRIRV
                     S"
     gene            complement(2796..4607)
                     /locus_tag="PLW15_05020"
     CDS             complement(2796..4607)
                     /locus_tag="PLW15_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HOT33795.1"
                     /translation="MRIRRLAAAAATVAAGALILSGCAAPQSEIVEGSSLAVAWNQPF
                     YSYNGLTSFGNATANNNITYATLSSFNYYDNTPALVKDTSFGSYEKISDDPLVVKYTI
                     ADGVKWSDGTAVDASDLLLQWAALSRALDTPDFDAAEFTDPDTGEFTSDFPTDVVYFD
                     SGADPTTGLGLVSTLPEVSDDKKSITLTYDAPFVDWELVFSAPLPAHVVAKNALGIDD
                     NQKAKDAIYDAIENDDKTALASISSFWNSGFNFTEMPSNPDLVLSSGPYVITDFVADQ
                     YVTLTANKQYTGANQAKVEEITVRFITDPLAAVQALQNGEVQVISPQATVDVADALAA
                     LDVTVIGGYEGTYEHIDLQFDQSKSGNFNNPLIREAFMKVIPRQEIVDKLIVPLQPDA
                     EVRNSQVFLPGAEGYDDSVANNGSAAYADVDIEGAKALLAQAGVTNPEVCMLFSSTNP
                     RRANEFLLIQQSGALAGFNVTDCSSPDWGGLLGTPGAYDASLFGWQSTSLGVTNGPPP
                     TFTTGGINNLNFYSNPEMDKLTAALSVEFDTAKQIELQKEIDKLLWSDFYGVTIFQFP
                     SVTAFDPNAIKGIAPSTLAPTIFWNIWDWEPVTAATE"
     gene            4861..5262
                     /locus_tag="PLW15_05025"
     CDS             4861..5262
                     /locus_tag="PLW15_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HOT33796.1"
                     /translation="MTEEGLSKPSKGPLLVFDGDCAFCTSAVNRLKAMLPVFPPTSPW
                     QWLDLDEYGLTTDDVTRYAWVVTSRHQYAGHLAGSALLRMQPTFGWRFLGHLIATPPI
                     SWVASLVYDWVAANRHRLPGGTPACAMPRAE"
     gene            5295..6065
                     /locus_tag="PLW15_05030"
     CDS             5295..6065
                     /locus_tag="PLW15_05030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT33797.1"
                     /translation="MDPLPSRGRLATEVLIVLGLSLGASAAYSVVSIVNRMTRTEALG
                     DQSTSINNPLSERPTFDLIYQLMGIFFDLVPVALCAFLLWQVTRPHLGRLGIDATRPG
                     SDFLRGLVLALLIGIPGIGVYLGGRALGITVTVVPNALDSYWWTVPVLILSALRAGVT
                     EEVIVIGYLYARLTDLGWGKWSIILGSATLRGTYHLYQGFGSFIGNFAMGVLFGWLYT
                     RWGRVLPLVIAHTLIDTAIFVGYPWAAATFPALFGLPG"
     gene            complement(6115..>6945)
                     /locus_tag="PLW15_05035"
     CDS             complement(6115..>6945)
                     /locus_tag="PLW15_05035"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490821.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOT33798.1"
                     /translation="ARFGDFGGDVSHLNLHKTFCIPHGGGGPGVGPVAAKAHLAPFLP
                     SHPMQQRAFTEALEVKGVTQHTVISAAPYGSPSILPISWAYVRMMGAEGLKAATGAAV
                     LAANYVAVRLREHYPVLYSGDNGLVAHECILDLRPLKEATGIDNNDVAKRLIDYGFHA
                     PTMSFPVPGTLMIEPTESEDLTEIDRFIDAMIAIKEEADAVAAGVWPADDNPLVHAPH
                     TAQSVIEGEWTHAYSRELAVYPLRSLIRGKYWPPVRRVDNAYGDRNLVCACPPVEAFA
                     "
     assembly_gap    6946..7045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7046..9149)
                     /gene="gcvP"
                     /locus_tag="PLW15_05040"
     CDS             complement(<7046..9149)
                     /gene="gcvP"
                     /locus_tag="PLW15_05040"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOT33799.1"
                     /translation="MFQDRHIGTDAAAQVTMLAAVGYPTVDALVNAAVPPSIHVKPLE
                     RSSIPAAASERDALGELRRLAGKNRVRRSMIGLGYYDTITPAVIKRNVLENPSWYTAY
                     TPYQPEISQGRLEALINFQTMVSELTGLATANASMLDEGTAVVEAMLLARRASGSASN
                     VFLVDADALPQTKALLRTRAEAVGIELREARWSSNGEAAYRDPADGFDTPSATQPAFG
                     ALIQYPGASGRVWDPRELIAAVQAQGGVAVVAADLLALTLLASPGDLGADVAVGTSQR
                     FGVPMGFGGPHAGYMAVRAGLERQLPGRLVGVSQDADGHPAYRLSLQAREQHIRREKA
                     TSNICTAQVLLAVMASMYAVYHGPHGLKRIAQSVNIRARLFARRVGVHGDYFDTVTVP
                     VPGRAAAIVADAGERGILLHLVDEDTVSVSFDETSADGSLDAALDEIFGLDASTDGPV
                     DGQSWPDSLTREGEYLTHPVFNTHRSETAMMRYLKYLADKDYALDRGMIPLGSCTMKL
                     NAATEMEAVTWPEFAGLHPFAPESDVEGYLQLIGELQDWLAEVTGYDSVSLQPNAGSQ
                     GELAGLLAIRGYHRSRGDLDRTVCLIPSSAHGTNAASAVLAGMRVVVVACDDLGNVDL
                     DDLRLKVAEHASDLAALMITYPSTHGVYEHEVRAICEAVHEAGGQVYVDGANLNALLG
                     YARFGDFGGDVSHLNLHKT"
     gene            complement(9173..9544)
                     /gene="gcvH"
                     /locus_tag="PLW15_05045"
     CDS             complement(9173..9544)
                     /gene="gcvH"
                     /locus_tag="PLW15_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT33800.1"
                     /translation="MADPKTLQYTAEHEWVLVDGAVATVGITAYAAEKLGDIVFVDLP
                     KVGSSVAEGKVVGEIESTKSVGELFAPVNGTVVEANDAVVASPELVNSDPFGEGWLIK
                     VEYAELPALLSFDEYSKLTGE"
     gene            complement(9537..>9862)
                     /locus_tag="PLW15_05050"
     CDS             complement(9537..>9862)
                     /locus_tag="PLW15_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOT33801.1"
                     /translation="ALTKEGDFVGRAAIEAGPPADARVLVGLRGEGKRAGRADYLIFD
                     GDDEVGIITSGALSPTLGYPIAMAYVDVASSAAGTTLEIDVRGSRVAASVVPLPFYKK
                     EENHG"
BASE COUNT         1691 a         3080 c         3338 g         1653 t
ORIGIN      
        1 ctggcctcgg gtgccgagcc gggaagcgtg cgccgcgaca tccattgggc caccatcgct
       61 gtgctcggtg gactgctcgt ggcgagcgtc ctgggcgtac tgctctagcc gcatcacgcg
      121 ctcttctcgg tgacgaacgt cgcccgcgag agcgccttgt ccgtgggcat gcgtcgctgg
      181 cgcgggtcga aggcatcccg aagtccgtct ccgatgaagt tgatgcacaa ggcgatcgcg
      241 atgatgaaca gacccggcca ccagaacagc cacggcctcg tggagaaggc gacctggttc
      301 tcgctgatga tcttgccgag cgaggtgtcg ggcgcctgca cgccgtaacc gaggaagctc
      361 aggccggtct cgatgaggat cgccgccgcc atgagcagcg tcgagttgac gatgatcacg
      421 ccgatggcat tcggcaggat gtgccggaag atgatgcgac ggtcctcgcg cccgcgacac
      481 gtgcggcatc gacgaactcc cgctcgcgca ggctgaggaa cgcgccacgc acgagtcgcg
      541 cgagaccggt ccagagcacg agcccgagga tgatcgccag aacaggcgct cccgagattc
      601 cgaagcgacg acccagcacg gcgccgatca cgatcaccgg gatggtgatg atgatgtcgg
      661 tgaagcgcat gagcaccgag tcggtccagc cgcggtagta gccggagatc gcgccgatga
      721 ccgtaccgat gaaggttgcg atcgcgccga cgatgaccat gatcatgagc gactgctgag
      781 tgccccgcat gacgacggcg aagatatcgc gaccgatctc atcctgaccg aaggggtggt
      841 tgccgatcgc gaacgggaag aggctgaggg tcggacgacc accgttctcg atcgcgaccg
      901 cgatgttcca ggtgtacggc caccagccgc cggacttcca actgccgaag gcgacaccca
      961 cagacgtgaa ggcgagcacg atgatgaagg cgagcacgac cattgagatc aatgcgccgc
     1021 ggtgccggaa gaaccgcttg cgcacgatct gaccctggct gaggccggcg atctccttga
     1081 gctcgatcga gttctcggcg tcggtgacgc cgccctcgtg atgtcccggc tgttcgacgg
     1141 gagggggaat ggtgctcatc agctcacccg gattctcggg tcaagacctg cgtaaaccag
     1201 gtcggcgatc agattgaaga ggatggccag cgatcccgtg acgaggaaga aggccatcac
     1261 agggttcgga tcgacgtgat cgagaccgtt ctggaagagg gcgcccatgc ccgtccagcc
     1321 gaacactcgc tcggtgatga ccgcgccgcc gatgatgccg ccgatgtcga acgcgacgat
     1381 cgtcgcgata gggatgagcg cattgcggaa ggcgtggcgc acgacgacgg tgcgctcggt
     1441 caggcccttt gcccgagccg tgcggatgta gtcctggttc atcacctcga gcaggctcgc
     1501 gcgggagtag cgggtgtacg tcgcgaacga gatgaggatg agcgcgagag tcggcagcag
     1561 gaggtgggtg aagagatcca ctccgtgaac ccagagcgag ccctcgagcc ccggtgtgcc
     1621 cgacccgatc gttgcgatcg gccgaccctt gatgcggccg gagttggcgt agtcatccca
     1681 gacctgcatg aagcggtcga gggagagcac ggagaaggcg acgaagccta cgatcgcggc
     1741 agtacgtgca acgggtcgtc ggtcgtcccc acccatgaac caacccacgg cgagcgccac
     1801 cagcacggtg gccacaccga tgccgatcca gccccagaac ggcaggccgg tcgtgaagta
     1861 gatgaacggg taccaggccg cgaccccgat tccggcaaca accagcgacg cgtacaacgc
     1921 ccgccgattc ttgagcccgg tggagatagt ggtcacgccg aaggccgcac cgacgagggt
     1981 gagcgcgact ccgacgatcc cgatgtgggg gtagctgaac cagtcggaga gatccatgta
     2041 gatgatcacg gcggccgagg cgagagcggc ggcggcaaac gtgatgagtt tgcggcgccc
     2101 cttgcccggc aggatcgaca tccagatcaa cccggatatc accgccaata tcgggatgat
     2161 ccacggggct atctggggat ctgacaggaa gtcgttgaag ccgatggcca cgaactgctt
     2221 gagcagcacg gcgacccaga agatcggcag ggagaagaac aggaacgaca ggaaggtcac
     2281 tccgtagtcg taaccgctgt actggcgaag cgcggtggtg ataccgacga ccagtccgag
     2341 gatgatggcg atgaccgagg cgatcgtcac gagctggagc gtggatccgg cagcctgggc
     2401 cagaagcacg gtgacgggct ggttctgcac agtgacgccg aggtcgcact ggccgatgaa
     2461 gcacttgccg acgccggcga tccagatgaa gtagcgcagg tagggcggca catcgaggtg
     2521 cagcagggcc gtgcgggctt cgatgagctg ctgcttgttg ggggcagtgc tggtgcgcag
     2581 gtcctcgagc gggtcccctg acaacgccgt caggatgtac atgaggtacg tggctgcgag
     2641 aaagatgaag atcgaggcga tcaagcgcct cacgatgaat gttgccactg acgggcacct
     2701 acctaacggg cttccaaggg tgggcgctgg ggcctgttac ggccccagcg ccctgggttg
     2761 tacctggcta ctgccaagtc atgagcgtga gctcattact cggtggcagc ggtgacaggc
     2821 tcccaatccc agatgttcca gaagatggtc ggcgcgagcg tcgagggcgc gatgcccttg
     2881 atcgcgttcg ggtcgaacgc cgtcaccgac gggaactgga agatcgtgac tccatagaag
     2941 tcactccaca gaagcttgtc gatctccttc tggagctcga tctgcttcgc ggtgtcgaac
     3001 tccacactga gggcagcggt gagcttgtcc atctcagggt tgctgtagaa gttcaggttg
     3061 ttgatgccac ccgtggtgaa cgtggggggc gggccgttgg tgacgccgag gctcgtcgac
     3121 tgccatccga agagggacgc gtcgtacgca ccgggggtac cgagcaagcc accccagtca
     3181 gggctggagc agtcggtgac gttgaagcca gcgagagcac cggactgctg gatcagcagg
     3241 aactcgttcg cgcgacgggg gttcgtcgac gagaagagca tgcagacctc ggggttggtg
     3301 acgccggcct gagccagcag cgccttggca ccctcgatgt caacatcggc gtacgcggcc
     3361 gagccgttgt tcgcgaccga gtcgtcgtag ccctcggcac ccggcaggaa gacctgcgag
     3421 ttgcggacct cggcgtcggg ctgcagcgga acgatgagct tgtcaacgat ctcctggcgc
     3481 gggatgacct tcatgaacgc ctcacggatg agggggttgt tgaagtttcc gctcttcgac
     3541 tggtcgaact ggaggtcgat gtgctcgtag gtgccctcgt agccaccgat gacggtgacg
     3601 tcgagtgcgg cgagcgcgtc agccacgtca accgtcgcct ggggcgagat gacctggact
     3661 tcaccgttct gcagggcctg tactgcggcc agcgggtcgg tgatgaagcg cacggtgatc
     3721 tcttcgacct tggcctggtt ggcgcccgtg tactgcttgt tcgccgtgag ggtcacgtac
     3781 tggtcagcga cgaagtcggt gatcacgtac gggccgctcg acagaacgag gtcggggttc
     3841 gacggcatct cggtgaagtt gaaaccggag ttccagaagc tcgagatgga ggcgagggcg
     3901 gtcttgtcat cgttctcgat ggcgtcgtag atcgcgtcct tggccttctg gttgtcgtcg
     3961 atgcccagcg cgttcttcgc cacgacgtgc gcgggaagcg gagcgctgaa gacgagctcc
     4021 cagtcaacga acggtgcgtc gtaggtgagc gtgatcgact tcttgtcgtc gctcacctcg
     4081 ggcagcgtgc tgacgaggcc gagacccgtc gtcgggtcgg ccccggagtc gaagtacacg
     4141 acgtcggtcg ggaagtcgga ggtgaactcg ccggtgtcgg ggtcggtgaa ctcggccgcg
     4201 tcgaagtccg gcgtgtcaag cgcgcgcgag agcgcggccc actgcaggag gaggtcggac
     4261 gcgtcgacgg cggtgccatc ggaccacttg acgccgtcag cgatcgtgta cttgaccacc
     4321 agcgggtcgt ccgagatctt ctcgtacgaa ccgaacgagg tgtccttcac gagggcgggg
     4381 gtgttgtcgt agtagttgaa gctactcaga gtggcgtacg tgatgttgtt gttcgccgtg
     4441 gcgttaccga aagaggtaag tccgttgtac gagtagaacg gctggttcca tgccaccgcc
     4501 agcgatgaac cctcaacgat ctccgactgc ggcgctgcgc agcccgagag gatgagagca
     4561 ccggcggcga ctgtggcggc agctgccgcg agtcgtctga ttctcaattt tcctcctgtg
     4621 caagatgagc aaacggcacg acaattccta ggccgcaccg cgcttggtag ttcacaccct
     4681 aaacgggggt ataacactgg caaaactgta gggggaaacg ttacacagtg gtaacgctct
     4741 ttggatttcc ttgcgcccct cgggggaatc gtgcgcgaat ctgtcgccaa acgcagttat
     4801 cttgcagatc gccctgcggg acgatctgaa tgagcgtgat tcacggcgta gcatcgaact
     4861 gtgaccgaag agggattgtc gaaaccatcc aaggggccac tgctggtctt cgacggggac
     4921 tgcgccttct gcacgtcggc cgtgaaccgg ctcaaagcca tgcttccggt gttccccccg
     4981 acctcgccct ggcaatggct agacctcgat gagtacgggc tcacgaccga cgacgtgacg
     5041 cgctacgcgt gggtcgtgac atcccggcac cagtacgccg gccacctcgc cggttcggcc
     5101 cttctgcgta tgcaaccgac cttcggatgg cgcttcctcg gtcacctcat cgctactccc
     5161 ccgatctcat gggtcgcctc cctcgtctac gactgggtcg ccgccaaccg ccatcgcctc
     5221 ccggggggca cccccgcctg cgcgatgccg cgagccgagt gaggctcgcg ccaggtccct
     5281 accccgccgc cgggatggac ccgttaccga gtcggggtcg gctcgccacc gaggtactga
     5341 tcgtcctcgg gctctcgctc ggagcatccg ccgcctactc ggtcgtctcg atcgtcaacc
     5401 ggatgacgcg caccgaggcc ctcggagacc agagcacctc gatcaacaac ccgttgagcg
     5461 agcgccccac gttcgacctc atctaccagt tgatgggcat cttcttcgat ctggttccgg
     5521 tcgcgctgtg cgcgttcctg ctctggcagg tcacccgccc gcacctgggg cggctcggta
     5581 tcgatgccac ccgccccggc tcggacttcc tgcgcggtct ggtgctcgcc ctgctgatcg
     5641 gcatccccgg catcggcgtc tacctcggcg ggcgggcgct gggcatcacc gtcacggtcg
     5701 tgccgaacgc cctcgacagc tactggtgga cggttcccgt gctcatcctc tcggcgctgc
     5761 gcgctggcgt caccgaggag gtcatcgtca tcggctacct ctacgcacgc ctgaccgacc
     5821 tgggctgggg caagtggtcg atcatcctgg gttcggcgac cctgcgcggc acctaccacc
     5881 tctaccaggg cttcggctcg ttcatcggca acttcgcgat gggcgtgctg ttcggctggc
     5941 tgtacacgag gtggggtcgc gtgcttccgc tggtgatcgc ccacacgctc atcgacacgg
     6001 cgatcttcgt cggctacccc tgggcggccg cgaccttccc ggctctgttc ggcctgccgg
     6061 gctgaccggt gatcgagcgg tgtcgagatc tcgacacgct cgatcaccca agcatcaggc
     6121 gaacgcctcc accggtgggc acgcgcacac caggttgcgg tcaccgtagg cgttgtcgac
     6181 acgacgcacc ggcggccagt acttgccgcg gatcagtgag cgcagcgggt agaccgcgag
     6241 ctcgcgcgag taggcgtggg tccactcccc ctcgatgacc gattgagcgg tgtgcggggc
     6301 gtgcacgagc gggttgtcgt cggcgggcca gacgccggca gcgacagcat ccgcctcctc
     6361 cttgatggcg atcatcgcgt cgatgaagcg atcgatctcg gtgaggtcct cgctctcggt
     6421 gggctcgatc atgagcgtgc cgggaacggg gaacgacatc gtcggggcgt ggaatccgta
     6481 gtcgatgagc cgcttggcca cgtcgttgtt gtcgatgccc gtcgcctcct tgagcggcct
     6541 gaggtcgagg atgcactcgt gcgccacgag accgttgtcg cccgagtaga gcaccgggta
     6601 atgctcgcgc aaccgcaccg ccacgtagtt ggcggcgagc acggccgcac cggtcgccgc
     6661 cttcagcccc tcggctccca tcatgcgcac gtacgcccac gagatgggca ggatgctcgg
     6721 ggagccgtag ggcgctgccg agatcacggt gtgctgggtc acgcccttca cttcgagagc
     6781 ctcagtgaat gcgcgctgct gcatcgggtg gctcggcagg aacggcgcga ggtgcgcctt
     6841 cgccgcgacc ggtcccacgc ccgggccgcc accaccgtgc gggatgcaga acgtcttgtg
     6901 caggttgagg tgcgagacat ccccaccgaa gtcgccgaac cgggcnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnacgtc ttgtgcaggt tgaggtgcga gacatcccca
     7081 ccgaagtcgc cgaaccgggc gtacccgagc agggcgttga ggttggcgcc atcgacgtag
     7141 acctggccac cggcctcgtg cactgcctcg cagatcgccc gaacctcgtg ctcgtacacg
     7201 ccgtgcgtcg acgggtaggt gatcatgagt gccgcaaggt cgctggcatg ctcggcgacc
     7261 ttgagccgca ggtcgtcgag gtcgacgttg ccgaggtcgt cgcacgcgac gacgaccacg
     7321 cgcatgcccg caagcaccgc gctcgccgcg ttggtgccgt gggcactcga cgggatcagg
     7381 cagaccgtgc ggtcgaggtc gccccgcgaa cggtgatagc cgcggatggc gagcaacccg
     7441 gccagttcac cctggctgcc ggcgttgggc tgcagcgaca ccgagtcgta gcctgtgacc
     7501 tcggcgagcc agtcctgcag ctcgccgatc agctgaaggt atccctcgac gtcggactcg
     7561 ggtgcgaagg ggtgcaggcc cgcgaactcg ggccaggtga ccgcctccat ctcggtggcc
     7621 gcgttgagct tcatcgtgca cgaaccgagc ggaatcatgc cccgatcgag cgcgtagtcc
     7681 ttgtcggcga ggtacttcag gtagcgcatc atggccgtct cgctgcgatg cgtgttgaac
     7741 acgggatgcg tgaggtactc cccctcgcgc gtgagcgagt cgggccagga ctggccgtcg
     7801 acgggtccgt cggtcgaggc atccaacccg aagatctcat cgagcgcggc gtcgagcgag
     7861 ccgtcggcgc tggtctcgtc gaagctcacc gagacggtgt cctcatcgac gaggtgcagc
     7921 aggatgccgc gctcgcccgc atcggcaacg atcgccgccg cgcgcccggg gaccgggacg
     7981 gtcacggtgt cgaagtagtc accgtgcacg cccacgcgcc gggcgaacag tcgagcgcgg
     8041 atgttcacgg actgcgctat gcgcttgagt ccgtgcggtc cgtggtacac cgcgtacatc
     8101 gaggccatga cggccagcag cacctgcgct gtgcagatgt tggaggtcgc cttctcgcgg
     8161 cggatgtgct gctcgcgcgc ctgcaacgag agccggtagg cggggtggcc gtcggcatcc
     8221 tgcgagacgc cgacgaggcg accggggagc tggcgctcga ggccggcccg cacggccatg
     8281 tagccggcgt ggggaccgcc gaagcccatg ggcacaccga accgctgact ggtgccgacg
     8341 gcgacgtctg caccgaggtc gcccggcgag gcgagcaggg tgagtgcgag caggtcggcc
     8401 gcgacgaccg caacgccgcc ctgggcctga accgccgcga tgagctcgcg cgggtcccag
     8461 acgcgaccgg atgcgccggg gtactggatg agcgcgccga acgccggttg agtagccgaa
     8521 ggcgtatcga aaccgtcggc cgggtctcga tacgcggctt cgccgttact cgaccagcgg
     8581 gcctcgcgca actcgatacc caccgcctcg gcgcgggtgc gcagcagtgc cttcgtctgc
     8641 ggcagcgcgt cggcatcgac gaggaagacg ttcgaggccg agcccgaggc gcggcgcgcc
     8701 agaagcatcg cctccacgac ggcggtgccc tcgtcgagca tcgacgcgtt ggctgtggcc
     8761 agcccggtga gctcggagac catcgtctgg aagttgatga gcgcctcgag tcgcccctgg
     8821 ctgatctccg gctggtaggg cgtgtaggcg gtgtaccagc tcgggttctc gagcacgttg
     8881 cgcttgatca ccgcgggcgt gatggtgtcg tagtagccga gtccgatcat cgatcgccgc
     8941 acccgattct tgccagcgag tcgacgcagc tcgccgagtg catcacgttc ggaggcggcc
     9001 gcggggatgc tcgagcgctc cagcggcttc acgtggattg agggcggcac ggcggcattc
     9061 acgagtgcgt cgaccgtcgg atagccgacg gcggccagca tggtgacctg ggccgcggca
     9121 tccgtgccga tgtggcggtc ctggaacatc tcgacaggct cgatgaccgc cactactccc
     9181 ccgtcagctt cgagtactcg tcgaaggaga gcagggcggg cagctcggcg tactccacct
     9241 tgatgagcca gccctcgccg aacgggtcgc tgttgaccag ctcggggctg gcgaccacgg
     9301 cgtcattggc ctcgacgacg gtgccgttga ccggagcgaa cagctcaccg accgacttgg
     9361 tcgactcgat ctcgccgacg accttgccct cggcaacgct cgagccgacc ttcgggaggt
     9421 cgacgaacac gatgtcgccc agcttctcgg ccgcgtaggc ggtgataccg actgtggcga
     9481 cagcgccgtc gacgagcacc cactcgtgct cggccgtgta ctggagggtc ttcggatcag
     9541 ccatggttct cttccttctt gtagaacggc agtgggacaa cggatgccgc aacgcggctt
     9601 ccgcggacgt cgatctcgag cgtggtgccg gccgcggagc tcgcgacatc cacatacgcc
     9661 atcgcgatcg ggtagccgag ggtcggggac agcgccccgc tcgtgatgat gccgacctcg
     9721 tcgtcaccat cgaagatcag gtagtcggcg cgaccggcgc gtttgccctc gccacgcagc
     9781 ccgacgagca cgcgggcatc ggccggcggg cccgcctcga tggcggcgcg gccgacgaag
     9841 tcgccctcct tggtcagcgc aa
//
LOCUS       DAOSOU010000063         9713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_6989_length_9713_cov_6.560157, whole genome shotgun sequence.
ACCESSION   DAOSOU010000063 DAOSOU010000000
VERSION     DAOSOU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6989_length_9713_cov_6.560157"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLW15_05055"
     CDS             <1..426
                     /locus_tag="PLW15_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT33802.1"
                     /translation="NDDAIRSVGLLTRIIADAAAEGLIQRHQKPEEGAEVEPLAAWEA
                     ELLQASETPVSEAEKIEAVDGEISGTPDAAAVAEVIAAEVPVEENDADALVAEHEAAA
                     DATVVPEHHVESDEATEAKIEAEATADEKKPAAKKPAAK"
     gene            456..1283
                     /gene="tsf"
                     /locus_tag="PLW15_05060"
     CDS             456..1283
                     /gene="tsf"
                     /locus_tag="PLW15_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT33803.1"
                     /translation="MADFSLEDLKTLRERLGTGMVDSKNALVEAGGDLEKATEILRLK
                     GAKSNAKRSDRSTSEGLVAAVESATATTLIELACETDFVAKSDKFIALGNAVLQALAD
                     SGAETVDAALAAKAGNGTVESLISDEAAILGEKIELRRLARIPGNKFAVYLHRTSKDL
                     PPQVGVVVAFEGDDAETIRSVAQHISFADPQYLTRDEVPAETVENERRIVEEISRGEG
                     KPEAALPKIVEGRVAAFFKQVALLDQDYAKDNKVSVGKVLSDAGLKVTGFARFKVGA"
     gene            complement(1372..1833)
                     /locus_tag="PLW15_05065"
     CDS             complement(1372..1833)
                     /locus_tag="PLW15_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOT33804.1"
                     /translation="MTRFENGPGESPGFLLWHTTMRWQRSMAAALHPLGLTHVQFVLL
                     ASAWWMSAGAPPNQVRLAAQAGTDIKMTSEVIARLEAKGLVTREPDPADARAKIVTVT
                     DAGAELAQRAIAVVEDADAEFFGRADTAAVVTVLRTLGDAEQMPAPGPRGS"
     gene            complement(1830..2252)
                     /locus_tag="PLW15_05070"
     CDS             complement(1830..2252)
                     /locus_tag="PLW15_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HOT33805.1"
                     /translation="MWTTAVTADTDLPRAAVWAALEALHRGELSYPGADVFELHGPFA
                     VGTELDVTPEGQDTFRSVIIELVPGERYADRTEYEGLSLLFRHTLVDAPTGTRVTHEL
                     EIDGAGADEVGPELGPQISGDFGAALEALFREAARMTA"
     gene            complement(2469..2957)
                     /locus_tag="PLW15_05075"
     CDS             complement(2469..2957)
                     /locus_tag="PLW15_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOT33806.1"
                     /translation="MPLRTALQDTRLIAALLTTAEQEARALGDAEPGAEHLLLAALLV
                     NDPSAREALATLAAVDAAAVRSAIVAVHSDSLADLGVAAPQLDPALPPASGAYRSEVS
                     AQEVFQRARILSRRSRSGLRSAHVLRAAAERERGTVARLLASLALERDAVLAAAAAAV
                     PD"
     gene            complement(2983..3198)
                     /locus_tag="PLW15_05080"
     CDS             complement(2983..3198)
                     /locus_tag="PLW15_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HOT33807.1"
                     /translation="MSEIENIPTPDDPDDGFAAVVSLRRLAATIERASVDRALEAGWT
                     WADIGQALGISAQAAHKRLSPGRRTPS"
     gene            3310..4026
                     /gene="pyrH"
                     /locus_tag="PLW15_05085"
     CDS             3310..4026
                     /gene="pyrH"
                     /locus_tag="PLW15_05085"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT33808.1"
                     /translation="MSEDKKRRVLLKLSGEAFGGGSLGVNPDVVSSLAKEIAAASAEG
                     VEVAIVVGGGNFFRGAELSQRGMDRGRADYMGMLGTVMNALALQDFLEQAGAATRVQS
                     AIAMTQVAEPYIPRRAERHLEKGRVVIFGAGAGLPYFSTDTVAAQRALEIDADVVLVA
                     KNGVDGVYDADPRKNSDAAKLEHVTYQEALVQGLKVVDSTAFSLCMDNSMPMVVFGME
                     PAGNVTKAIRGDRIGTLVSN"
     gene            4062..4613
                     /gene="frr"
                     /locus_tag="PLW15_05090"
     CDS             4062..4613
                     /gene="frr"
                     /locus_tag="PLW15_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT33809.1"
                     /translation="MISDVLAEAKDKMGKAVEAAKDDFATVRTGRANPALFQKVLVDY
                     YGTPTPLAQLASMVNQEARTLLITPFDKSALKDIEKAIAGASHLGASVGNDGAVIRAT
                     LPELNEERRKEFVKLVRDKAEQARVAIRNIRRKAKDDLDSLKEVGEDEISRAEKELDA
                     TTKQHVDAIDDAVKRKEAELLEV"
     gene            4613..5488
                     /locus_tag="PLW15_05095"
     CDS             4613..5488
                     /locus_tag="PLW15_05095"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT33810.1"
                     /translation="MASDFEAQVRATNDKINARTGRPLIRAIAVGLILGGALLVSLVL
                     VKELYMVFAAALIGFTSYELASALRFAGRDVPRLPTVIVAVALVPASFYWLDAGHWYV
                     TLAGIAFLSIWRIVELARPSHRVAPIELLKDLAAGAFIMAYVAFMGAFTVLLTAQDGG
                     QWWTLAFLIVVVAVDTGAYATGVAFGKHPMAPTISPKKTWEGFAGSVAAAVIAGVLLS
                     IFMLHQPWWVGVILAVTIVATATSGDLAESLIKRDLGIKDISTWLPGHGGFLDRVDSA
                     LLSAAAAYALFLIFA"
     gene            5541..6092
                     /locus_tag="PLW15_05100"
     CDS             5541..6092
                     /locus_tag="PLW15_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT33811.1"
                     /translation="MSTTFPRARKSKPGYNVDQVEDFLEEARRAYTADRGDLGVVTAA
                     SIRRTAFALQKGGYSTSHVDAALERLEDAFAARERERAFATTGDAAWYAQARSSAQEI
                     IDRLARPEGQRFNRVGPFTTGYSPKDVDAFTNRLVGYFQAGKPMSVDHVRTVVFGSKK
                     RGYNEQQVDLLLDAVIDVMLAVR"
     gene            6150..6764
                     /locus_tag="PLW15_05105"
     CDS             6150..6764
                     /locus_tag="PLW15_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOT33812.1"
                     /translation="MGISTRAIELRAQLKSEGGDVSGRHRRAPWSLPAFATFVTFAFL
                     AVTLAPPPTGSTYAATSPQRSGETQSVDVAGDYSLSAERDAFSVSEIRVAPTAPAVGI
                     PDAGSAQAIALEMVLARGWDMTEFSCLVALWTRESNWNAYSHNSSSGAYGIPQALPGE
                     KMASAGDDWATNPATQITWGLGYITDRYGTPCGAWGHSEEKGWY"
     gene            6810..8546
                     /locus_tag="PLW15_05110"
     CDS             6810..8546
                     /locus_tag="PLW15_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT33813.1"
                     /translation="MELLQLGVYGVIGLVTIVAAGAFSARLGIAAPLVLIVVGVGFSY
                     IPGAPTSVPHELILLGLLPPILYAASITVPVVDFRRNFSTISALSVGLVIVTTAVTGY
                     LLYLIFPQLSLAAAIAIGAVISPTDAVAATALGKKFGLPPRLVTIMEGESLVNDATAL
                     VLLRSAIAATASAVSFWDVAGDFLFAVAVAAAIGLAVGFVTVWVRSKITDPLLDTAIS
                     FAVPFIAFIPTQFVGASGVLAVVVAGLYSGHHGARRFSAQARVAERLNWRTLQFVLEN
                     GVFLLMGVQISGLISSVIQDDLSAGLAVVLGVLMTAVVIVIRFLFVGPVLLLLRAELR
                     RAERAQTRFGEFLDQFRRRSDVSARGVRRQQRAEAIHERRGQDLAQLQAEGLGWRGGV
                     ILGWSGMRGVVTLAAAQSLPDTTPYRPQLVLIAFTVAVVTLLLQGGTLPWLIRLTGIR
                     GADAVADSRELAGLLDELAESGLAAIESPSLELPEGQVIDEHVLERVRSDTLLRAQSA
                     WERVERTPTSPVSPQRQYRALRREVLRAEREALLEARSAGRYPSRILIRAQAMLDLEE
                     TRLEQIDNPGGA"
     gene            8583..8885
                     /locus_tag="PLW15_05115"
     CDS             8583..8885
                     /locus_tag="PLW15_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33814.1"
                     /translation="MPRSNRPRGRRPDAEAVTEVDLSRALLGRMHTEPRRDGLWNVQP
                     LAASSSTKVYTCPGCSLEIAPGTAHIVAWRADGLMGEADDLAARRHWHTHCWKIRP"
     gene            8891..9601
                     /locus_tag="PLW15_05120"
     CDS             8891..9601
                     /locus_tag="PLW15_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33815.1"
                     /translation="MEIRGGIELPARRENVELETSDGLTLVGELSLPELAPPVATLVA
                     LHPLPTAGGFMDSHIIRKAAARLPALADIAVLRFNFRGVTSPRGTSEGEFGHGLDEQH
                     DLAAAMDLVQSRGLPHPWIVGWSFGTEVALKWGLEHPIEGAILLSPPLHRTTPAELGA
                     WAGSGKRLVAVIPELDDYLRPAEAAERFAAVPETEFVAVEGGKHLWVGEEQTYRVLTE
                     IVARLNPGVLPLPRTWEG"
BASE COUNT         1527 a         3293 c         3331 g         1562 t
ORIGIN      
        1 aacgacgacg cgatccgctc ggtgggcctg ctcacccgca tcatcgccga cgccgccgcc
       61 gaggggctga tccagcgtca ccagaagccc gaggagggcg ccgaggtcga gcccttggcc
      121 gcgtgggaag ccgagcttct ccaggcgtcg gagacccccg tctcggaggc cgagaagatc
      181 gaggccgtcg acggtgagat ctccggtacg ccggatgccg cggctgtcgc cgaggtcatc
      241 gccgccgagg ttcccgtcga ggagaacgac gccgacgcgc tcgtcgctga gcacgaggcc
      301 gccgccgacg cgaccgtcgt tcccgagcac cacgtcgagt ctgacgaggc gaccgaggcc
      361 aagatcgagg ccgaggccac tgccgacgag aagaagcccg cggccaagaa gcccgctgcc
      421 aagtagttca ctccaaccca gttaggaccc acaccgtggc agatttcagc ctcgaagacc
      481 tcaagaccct gcgcgagcgc ctcggcaccg gcatggtcga cagcaagaac gccctcgttg
      541 aggcgggtgg tgacctcgag aaggccaccg agatcctgcg tctcaagggt gcgaagtcca
      601 acgccaagcg cagcgaccgc tcgaccagcg agggccttgt cgccgctgtc gagtcggcga
      661 ccgcgaccac gctgatcgag ctcgcctgcg agaccgactt cgtggccaag agcgacaagt
      721 tcatcgcgct cggcaacgcc gtgctccagg ctctcgccga ctcgggcgcc gagactgtgg
      781 atgccgcgct cgccgccaag gccggcaacg gcacggtcga gtcgctcatc tccgacgagg
      841 ccgcgatcct cggcgagaag atcgaactgc gtcgcctcgc gcgcatcccc ggcaacaagt
      901 tcgccgtcta cctgcaccgc accagcaagg acctgcctcc gcaggtcggt gtcgtggttg
      961 cgttcgaggg tgacgacgcc gagactatcc gctcggttgc gcagcacatc tcgttcgccg
     1021 acccgcagta cctgacccgc gacgaggttc cggccgagac ggtcgagaac gagcgtcgca
     1081 tcgtcgagga gatcagccgt ggcgagggca agcccgaggc cgccctcccg aagatcgtcg
     1141 agggtcgcgt ggccgcgttc ttcaagcagg tcgccctgct cgaccaggac tacgccaagg
     1201 acaacaaggt ctccgtgggc aaggtcctgt cggatgccgg actgaaggtc acggggttcg
     1261 cccgcttcaa ggtcggcgcc taggctccca gcttcaccag caacgccccg caggtcgcga
     1321 gaccggcggg gcgttgtcgc gttcgggatg ccgcggctca ggttgcgcga gtcaactgcc
     1381 gcgcggtccg ggcgcgggca tctgctcggc atcgccgagc gttcgcagca ccgtgaccac
     1441 cgctgcggtg tccgcccggc cgaagaactc ggcgtcggcg tcttcgacga cggcgatcgc
     1501 gcgctgcgcg agttctgccc cggcatcggt gacggtgacg atcttggcgc gcgcatccgc
     1561 cggatcgggt tcacgggtga cgagcccctt cgcctccagc cgcgcgatca cctcggaggt
     1621 catcttgatg tcggtgcccg cctgggccgc cagccgcacc tggttcgggg gagccccggc
     1681 ggacatccac caggcgctcg cgagcagcac gaactgcacg tgggtgagcc cgagcgggtg
     1741 cagcgctgcc gccatggacc gctgccagcg catggtcgtg tgccacagca ggaagccggg
     1801 gctctcgccg gggccgttct cgaagcgggt cacgcggtca ttctcgcggc ctcgcggaac
     1861 agcgcctcga gcgccgcgcc gaagtcgccg ctgatctgcg ggccgagctc gggcccgacc
     1921 tcatcggctc ccgccccgtc gatctcgagt tcgtgggtga cacgggtgcc ggtgggggcg
     1981 tcaacgagcg tgtgacggaa cagcaggctg aggccctcgt actcggtacg gtcggcgtac
     2041 cgttcgcccg gtacgagctc gatgatgacc gagcggaagg tgtcctggcc ctcgggcgtc
     2101 acgtcgagct cggtgcccac ggcgaacggt ccgtgcagtt cgaacacgtc ggcacccgga
     2161 tagctcagct ctccgcgatg gagtgcctcc agggcggccc agacggctgc ccggggtagg
     2221 tcggtgtcgg cggtgacggc ggtggtccac atgggggttc ctttcggtaa ataatctacg
     2281 cacagactat ctgtggacag atgaaatgcg caaggccccg gccccaggcg tcgatcaatc
     2341 ccttgccgat cggccgcacc gcggattccg tggaatttca ggtgatgaca atcgccgtcc
     2401 agtggtccgg cgtgacccgc tgagcagcat catcacggcg gccgtcgccg tgccgaggat
     2461 cgcggccctc agtcgggcac cgccgcggct gcggcggcca gcacggcgtc ccgctccaag
     2521 gcgagggacg ccagaagccg ggcaacggtt ccgcgttcgc gctcggcggc ggcccgcagc
     2581 acgtgggcgc tgcgcaggcc gctgcgcgag cggcgcgaga ggatccgagc gcgctggaac
     2641 acctcctgcg ccgagacctc gctccggtag gcgccggagg cgggcggcag ggcggggtcg
     2701 agctgggggg cggcgacgcc gagatcggca agggagtccg aatgcacggc gacgatagca
     2761 gagcgcacgg ccgcggcgtc caccgccgcg agagtggcga gcgcttcgcg cgccgacggg
     2821 tcgttcacga gcagcgcggc gagaagcagg tgctcggcgc cgggctcggc gtcgccgagg
     2881 gcgcgcgctt cctgctcggc ggtggtgagg agggcggcga tcagtcgggt gtcctggagg
     2941 gcggtgcgga ggggcatggg ttcctttcgg gagtgtcggg tttcaggagg gggtgcggcg
     3001 cccgggcgat agccgcttat gggcggcctg ggctgaaatg ccgagcgctt ggccgatgtc
     3061 ggcccacgtc cagccagcct cgagtgcccg gtcgacgcta gcgcgctcga tagtcgcagc
     3121 gagtcggcgc aatgacacga cagcggcgaa gccgtcatcc ggatcgtcgg gcgtcggaat
     3181 gttctcaatc tcggacatgc atcaacaatg gttgacggct cgcgcggtgt caaccattgt
     3241 tgatgaatcg cggcgtcgca aaccgcgcgc accgaatacg ctggaggggc cgcacgagag
     3301 agggagcgca tgtccgagga caagaagaga agggtgctcc tgaagctctc cggtgaggca
     3361 ttcggcggcg gctcgttggg ggtcaacccc gatgtggtct cctcgctcgc gaaggagatc
     3421 gccgcggcat ccgctgaggg ggtcgaagtc gcgatcgtcg tcggcggcgg caacttcttc
     3481 cgaggcgccg agctcagcca gcgcggcatg gaccgcggcc gcgccgacta catgggcatg
     3541 ctcggcaccg tgatgaacgc gctcgcactc caggacttcc tcgagcaggc gggggccgcc
     3601 acacgcgtgc agtcggccat cgcgatgacc caggtcgccg agccgtacat cccccgtcgt
     3661 gcggagcggc acctcgagaa ggggcgcgtc gtgatcttcg gagccggtgc cggtctgccc
     3721 tacttctcca ccgacaccgt cgccgcccaa cgcgccctgg agatcgacgc tgatgtcgtg
     3781 ctcgtcgcca agaacggtgt cgacggtgtg tacgacgcag atccgcgcaa gaactccgac
     3841 gcagccaagc tcgagcacgt cacctaccag gaggcactgg ttcagggact caaggtcgtg
     3901 gactcgaccg ccttcagcct gtgcatggac aactcgatgc ccatggtggt cttcggaatg
     3961 gagccggcgg gcaatgtcac caaggcgatt cgcggcgacc ggatcggcac tctcgtttcc
     4021 aactagacct ctaaacttgg gttcgacaca agggagttgc cgtgatcagc gacgtattgg
     4081 ccgaagccaa ggacaagatg ggcaaggccg tcgaggccgc taaggacgat ttcgcgacgg
     4141 tgcgcaccgg acgagcgaac ccggccctgt tccagaaggt gctcgtcgac tactacggca
     4201 cgccgacccc gcttgcgcag cttgcctcca tggtcaatca ggaggcccgc acactgctca
     4261 tcaccccgtt cgacaagtcg gccctcaagg acatcgagaa ggccatcgcg ggtgcctcgc
     4321 acctcggtgc gagcgtcggc aacgacggcg ccgtcatccg cgcgacgctg cccgagctca
     4381 acgaggagcg ccgcaaggag ttcgtgaagc tggtgcgcga caaggcggag caggcgcgcg
     4441 tggcgatccg caacatccgt cgcaaggcga aggacgacct cgactccctc aaggaggtcg
     4501 gcgaggacga gatctcccgc gcggagaagg aactcgacgc caccaccaag cagcacgtcg
     4561 acgcgatcga cgacgctgtc aagcgcaagg aagccgagtt gctcgaggtc tgatggcgtc
     4621 cgacttcgag gcccaggttc gcgcgacgaa cgacaagatc aacgcgcgca ccggccgccc
     4681 cctcattcgc gcgatcgccg tcgggctcat tctcggcgga gccctgctgg tcagcctcgt
     4741 gctcgtcaaa gagctctaca tggtcttcgc ggcggccctc atcgggttca ccagctacga
     4801 attggccagt gcgctgcgct tcgccggccg ggatgtgccc cgcctgccca cggtcattgt
     4861 cgcggtcgcg ctcgtgccgg cctcgttcta ctggctggac gccggccact ggtacgtgac
     4921 cctcgcgggc atcgcgtttc tgtcgatctg gcgcatcgtg gagctggctc gcccgagcca
     4981 ccgggtcgcg ccgatcgagc ttctcaagga tctggcggcg ggcgccttca tcatggccta
     5041 cgtcgcgttc atgggcgctt tcaccgtgct tctcactgcg caggacggtg gccagtggtg
     5101 gacgctggcc ttcctgatcg ttgtggtcgc ggtcgacacc ggtgcgtacg cgacgggtgt
     5161 cgcattcggc aagcacccga tggcaccgac gatcagcccc aagaagacct gggaggggtt
     5221 cgcaggatcg gtcgcggctg ccgtgatcgc cggcgtgctg ctgtcgatct tcatgcttca
     5281 ccagccctgg tgggtgggcg tgatcctcgc ggtcaccatc gttgcgaccg cgacctcggg
     5341 cgatctcgcc gaatcgctga tcaagcgcga cctgggcatc aaggacatct cgacctggct
     5401 tcccggccac ggcggtttcc tcgaccgtgt cgactcggcg ctgctgtcgg ccgctgccgc
     5461 ctacgcgctg ttcctgatct tcgcgtgagc ccgcgctcac gctgacacgg ccctcaggca
     5521 acgtagggca agatagtcgg gtgagcacaa ctttcccgcg cgcccgcaag tcgaaacccg
     5581 gctacaacgt cgaccaggtc gaggacttcc tcgaggaggc ccgtcgcgcc tacacggccg
     5641 accggggcga tctcggtgtt gtgaccgccg cgagcatccg tcggaccgcc ttcgccctgc
     5701 agaagggtgg ctattcgacc agccatgtgg atgccgccct cgagcgtctc gaggacgcct
     5761 tcgcggcccg cgagcgcgag cgcgcattcg cgacgaccgg ggatgccgcg tggtacgcgc
     5821 aggcgcgcag ctcggcgcag gagatcatcg atcggcttgc ccgccccgag gggcagcggt
     5881 tcaaccgggt cggcccgttc acgacggggt acagccccaa ggacgtggat gcgttcacca
     5941 accgcctcgt gggctacttc caggctggca agccgatgag cgtcgaccac gttcgcacgg
     6001 tcgtcttcgg atcgaagaag cgcggctaca acgagcagca ggtggacctt ctgctcgacg
     6061 cggtcatcga cgtcatgctc gcggtgcgct gacccgaccg aacggcctcc cggggctctg
     6121 acaggcccat gggctaaaat catgcaactg tggggataag cacgcgggcg atcgaactcc
     6181 gtgcccagct caagagcgag ggcggagacg tttctgggcg ccatcggcgc gccccgtggt
     6241 cccttcctgc cttcgccacc ttcgtcacat tcgccttcct cgccgtgacc ctcgccccgc
     6301 ctcccaccgg ctcgacttac gcggcgacga gcccgcagcg cagcggtgag acccagtcgg
     6361 tcgacgtcgc tggtgactac tccctgtcgg ccgagcggga tgcgttcagt gtgagcgaga
     6421 tacgggtcgc gccaaccgcg ccagcggtgg gcattcccga tgcggggtcg gcgcaggcca
     6481 ttgccctgga gatggtgctc gcccgcggct gggacatgac cgagttcagc tgcctcgtcg
     6541 cactgtggac ccgtgagtcg aactggaacg cctactcgca caactcgtcg agcggtgcct
     6601 acggcatccc gcaggccctg cctggcgaga agatggcgtc ggccggcgac gactgggcca
     6661 ccaacccggc cacccagatc acctggggtc tcggctacat caccgaccgc tacggcaccc
     6721 cgtgcggtgc gtggggccac tcggaggaga agggctggta ctagccagcc gggtctgagc
     6781 gtggcatcga cggccactac gctggccgga tggagttgct gcagctcggt gtgtacgggg
     6841 tcatcgggct ggtcacgatc gttgccgccg gtgcgttcag tgctcgattg ggcattgcag
     6901 cccctctcgt actcatcgtc gtcggcgtcg gattcagcta catcccgggc gcacccacgt
     6961 cggtgccaca tgagctgatc ctgctcgggc tgctgccacc gattctgtac gcggcatcca
     7021 tcacggtccc cgtcgtcgac ttccgtcgca acttcagcac catctccgca ctcagcgtgg
     7081 ggctcgtcat cgtgaccacc gcagtgacgg gctacctgct ctacctgatc ttcccgcagc
     7141 tgtcactggc ggcggccatc gcgatcggcg cggtcatcag ccccaccgac gcggtcgcgg
     7201 caacggcgct gggcaagaag ttcggacttc cgccgcgact cgtgacgatc atggagggcg
     7261 agagtctggt caacgatgcg accgcgctgg ttctgctgcg gtcggcgatc gccgccacgg
     7321 catccgccgt ctcgttctgg gatgtcgctg gtgacttcct cttcgctgta gccgtggccg
     7381 cggcgatcgg gctcgccgtc ggattcgtca cggtctgggt gcgctcgaag atcaccgatc
     7441 cgcttctgga caccgccatc tcgttcgctg tgccgttcat cgcgttcatt cccactcagt
     7501 tcgtcggggc atccggggtg ctcgccgtcg tcgtcgcggg cctctactcg ggccaccacg
     7561 gggcacgccg gttctcggcg caggcgcgcg tcgccgagcg gctcaactgg cgcacgctgc
     7621 agttcgtgct cgagaacggc gtcttcctgc tcatgggagt gcagatctcg gggctgatct
     7681 cgagcgtcat ccaggacgat ctgagtgcgg ggctcgccgt cgtgctcggg gtgttgatga
     7741 ccgcggtcgt catcgtgatc cgcttcctct tcgtcggacc cgtgctcctg ctgctccgag
     7801 ccgagctgcg ccgggccgag cgggcccaga cccgcttcgg cgagttcctc gatcagttcc
     7861 ggcgcaggag cgatgtgagc gcccgaggag tcaggcggca acaacgagcg gaggccatcc
     7921 acgagcgtcg agggcaggat cttgcccagc tgcaggcgga gggcctgggc tggcggggcg
     7981 gtgtgattct cgggtggtcc gggatgcgcg gggtcgtcac gctcgcggcc gcccagtccc
     8041 tgccggacac gacaccctac cgaccccaac tggtgctcat cgcgttcacg gttgcggtcg
     8101 tgacgctact gctgcagggc ggtaccctgc cgtggctgat caggctgacg ggcatccgtg
     8161 gggcggatgc ggtcgccgac agtcgtgagc tcgccggact gctcgacgaa ctcgcggagt
     8221 ccggactggc cgcgattgag agccccagtc tcgagcttcc ggagggccag gtgatcgacg
     8281 agcacgtgtt ggagcgggtg cgcagcgata ccctcctgcg tgcccagtcg gcgtgggagc
     8341 gggtggaacg cacccccacg agtcccgtgt ccccccagcg ccagtatcgc gcgctgcgca
     8401 gggaggtgct ccgtgctgag cgagaggcgc tgctggaggc caggtcggcc ggccgatacc
     8461 cctcccggat cctcattcgc gcgcaggcga tgctcgacct cgaggagacc agactcgagc
     8521 agatcgacaa ccccggcggc gcctgacgcc gtccctccga ttcacgctcg tagactggtc
     8581 tcatgcctcg ttcgaatcgt ccccgcggcc gccgcccgga cgccgaggcg gtcaccgaag
     8641 tcgacctcag ccgagcatta ctggggcgga tgcacaccga gccgcgtcgc gacggcctgt
     8701 ggaatgttca gccgctcgcg gcatccagct caaccaaggt ctacacctgc cccgggtgca
     8761 gcctcgagat cgccccggga acggcccaca tcgtggcgtg gcgagcggac gggctgatgg
     8821 gcgaagccga cgacctcgcg gcccgccggc actggcacac gcactgctgg aagatccgtc
     8881 cgtgacggcg gtggagatcc gcggcggcat cgagttgccc gcccggcggg agaatgtcga
     8941 gctggagacc agcgacgggc tgaccctggt gggggagttg agcctgcccg aactcgcacc
     9001 gccggtcgca accctggtgg cgctgcatcc gctgcccacc gccggcggtt tcatggattc
     9061 gcacatcatc cgcaaggccg cggcccgact gcccgcactc gccgacatcg ccgtgctgag
     9121 gttcaacttc cgtggggtca catcgccgcg gggaacctca gagggcgagt tcggtcatgg
     9181 gctcgacgag caacacgacc tggccgcagc gatggacctc gtgcagtctc gtggccttcc
     9241 gcacccgtgg atcgtggggt ggtcgttcgg caccgaggtc gcgctcaagt ggggactcga
     9301 gcatccgatt gagggagcca tcctgctgtc gcctccgctg caccgcacca caccggccga
     9361 gctcggcgcg tgggccggta gcggcaagcg actcgtggct gtgattcccg agctcgatga
     9421 ctacctgcgc cccgccgagg ccgccgagcg gttcgccgcc gtgcccgaga ccgagttcgt
     9481 cgccgtcgag ggtggtaagc atctctgggt cggcgaggag cagacctacc gcgtgctcac
     9541 cgagatcgtg gcgcgactga accccggtgt gctgcccttg ccgcgcacct gggaaggctg
     9601 atcgagtctg cggtgatcga gtctgcggtg atcgagcccg tcgagatagg gcctgccgag
     9661 atcgagcctg tcgagatccg gccctagagc tcttcctgcc gaggcacgaa cac
//
LOCUS       DAOSOU010000064         9700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7004_length_9700_cov_5.206739, whole genome shotgun sequence.
ACCESSION   DAOSOU010000064 DAOSOU010000000
VERSION     DAOSOU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7004_length_9700_cov_5.206739"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PLW15_05125"
     CDS             complement(<1..962)
                     /locus_tag="PLW15_05125"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT33816.1"
                     /translation="MTSLDDLPIRDDLRGLEPYGAPQKHVRVQLNVNENTHAIPEAVA
                     TDIVMALAGAVMGVNRYPDREFTELRDSLAGYLGHGLTRDNIWAANGSNEVLQQTLQA
                     FGGPGRSVLGFPPTYSMHSIIASGTGTRWVTAERDDGYELSPQTVVAAIERKQPDIVF
                     LCSPNNPTGTPLGLDTIEAAYDATPGILLVDEAYAEFADGDTALTLLEGRPRLLISRT
                     MSKAFAFAGARLGYLAADPAVTDALRLVRLPYHLSALTQAAAIAALAHAPEMLAMVED
                     IKHQRDRMILALPALGYTPLPSAANFVLFGGVADPHAVFEALLER"
     gene            complement(1002..1385)
                     /locus_tag="PLW15_05130"
     CDS             complement(1002..1385)
                     /locus_tag="PLW15_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT33817.1"
                     /translation="MSTAVIAPGYGFTPARTAQSVPASAPKLRLTKRGRAVLTFLAAT
                     PLVIAALVFALNGGGATASLEGSSVGFEYVTVESGQTLWQLAESIAPAADPRDVIGEI
                     MKLNLLESADVYAGQELAIPAAYAH"
     assembly_gap    1634..1733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1734..2183
                     /gene="lexA"
                     /locus_tag="PLW15_05135"
     CDS             <1734..2183
                     /gene="lexA"
                     /locus_tag="PLW15_05135"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOT33818.1"
                     /translation="MEVLIDLPSLEGADATAESTSPAVGDAVMVPLVGRIAAGIPITA
                     DQQVDEVFPLPRQLTGKGELFMLKVVGESMIDAAICDGDWVVVRQQQTAENGDIVAAM
                     LDSEATVKVFRQRDGHTWLLPRNSNFEPILGDHAVVLGKVVTVLRAL"
     gene            2231..5851
                     /gene="metH"
                     /locus_tag="PLW15_05140"
     CDS             2231..5851
                     /gene="metH"
                     /locus_tag="PLW15_05140"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOT33819.1"
                     /translation="MASSVTSPSIADLSDIPRPARSDALMETLRQRVVIADGAMGTML
                     QDAGPTMEDFQQHEGCNEILNVSRPDIVETIHHAYLDTGIDAIETNTFGSNWSNLSDY
                     GIEDRIEELSRAGAEIARRAVETYEARDGRMRWVLGSIGPGTKLPSLGHTTYDNLKLT
                     FGEQAKGLILGGSDALMIETSQDLLQTKAAVNGCKLAMVETGIRLPIFVEVTVETTGT
                     MLMGSEIAAALTTLEPLGIDMIGLNCATGPDEMSEHLRHLSKFAHIPVMCMPNAGLPV
                     LGPNGASYPLTPDELATAHEQFVKEFGLGLVGGCCGTTPEHLKAVVDRIGGARALTRT
                     IEEDNGVASLYQHVPFTQDSSYLAIGERTNANGSLAFRAAMLEERWDDCVDIARSQVR
                     EGANLLDVCIDYVGRDGVADMREIVSRLASASTLPLVVDSTEPAVLQAGMELIGGRPV
                     VNSVNFEDGDGPQSRFGRIMPLVKEHGAAVIALTIDEEGQARTAEHKVRIASRLVDSL
                     VGEWGMRVDDIIVDALTFPIATGQEETRRDAIETIEAIRQINAKYPGIHTTLGVSNVS
                     FGLNPAARSVLNSVFLHEAVEAGLDSAIINAAKIVPLASVPDDRRKVALDLVWDKREY
                     DADGTVTYDPLSAMLDLFDGVDSAALKDQRAAELAALPVGQRLERRIIDGEAKGLEAD
                     LDLARADGMSALSIINDHLLAGMQVVGQRFGSGEMQLPFVLQSAEVMKAAVALLEPHM
                     EKSDAAGKGTMVIGTVRGDVHDIGKNLVDIILTNNGYDVINIGIKQPLSEFIRAAEEH
                     NADVIGMSGLLVKSTVVMKENLQELSARGLGTRWPILLGGAALTRTYVEDDLASIFDG
                     EVRYARDAFEGLALMEPLVAIARGAARDSVGLPPLKKRIHARTTIDVTEPENMPQRSD
                     VVTDNPIPAPPFWGTRIVRGIALADYAAFLDERATFMGQWGLKPSRAEDGASYEELVQ
                     TEGRPRLRYWLDRILAEGMIDASVDYGYFPAYSEGDDLVILHHGDDPTGILGTPGLLA
                     PDGGSGGPIGTERLRFSFPRQRRDRHLCLADYVRSRESGQVDVVALQLVTAGSAVDAF
                     TAQMFAENKYRDYLELNGLTMQLTEALAEYWHSRVRAELGFAAEDPTDIAGMFKLNYR
                     GARFSLGYPACPDMEDRRKVVELLKPERMGVELSEELQLHPEQSTDAFVFHHPEARYF
                     SV"
     gene            complement(5739..6671)
                     /locus_tag="PLW15_05145"
     CDS             complement(5739..6671)
                     /locus_tag="PLW15_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT33820.1"
                     /translation="MKYRVVAVVGARREVSSRVTLLIASLLALAGFLWPLIATAVPRD
                     AQAAVPVLALALVPVAVVALALTVDRQMHSATTVALLGMLTAVGAAVRIAGTGVGGLE
                     AVFIVLILAGRAFGARFGFLLGMLVISLSSVLWGGIGPWTPFQIFACGWVAAGAGLLP
                     RITARSQRRAARLEVLMLVGYGILASYAFGFIMNLWFWPFAVGAGSDISYSAGAPLGQ
                     NLASFGLYSLVTSTLTWDTVRAVTTVVGIVLVGPAALAALRRARIGGRRTRRASGAIP
                     RSTVPRGDGTRTRQSTARDEAEAPQTVPRPSARA"
     gene            complement(6668..8611)
                     /locus_tag="PLW15_05150"
     CDS             complement(6668..8611)
                     /locus_tag="PLW15_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33821.1"
                     /translation="MRLTRLAALRTAILVAVGFVVVRVLYRIVFGGADGPGMLLLDLP
                     RVRLSGPFEHITLLGPVTSGGVALIAWSAVPFALLVLAFGLLAVVFDLRALAARGAVR
                     GPVRTISRALVVAWGAVPALVGSVARVRTARRLRGQRGVASLLVPVLEQSVERAIALG
                     SAMEVRGFAATRRAEPECERPASVRSASLGYDGSWSLVDLDLDVSPGTLTVVTGATGS
                     GKSTLLDALSGLFQHVAGGEQQGIVEVAGVNRALVSPRETAGLVGVVAQSVRLSFVAA
                     TVREELGFALANRDVAAVIVTARVEETARRLGIQHLLTRSTVALSAGEACLVAIGAAL
                     IEHPVLLLVDEPLADLDDSARVMVIDLLDELAHQAGVAVVVAEHAIRGWGDRADVWLE
                     VSDGSVIRRTGAELALRDSARTQPRHADLGPAPVSAAPIAEIRDLTVTHGDEVAVAGA
                     SLHLCGGEVVALRGPNGAGKSSLLLATALPTQRGAVFVDGCDVHAVGRRERPSYVALV
                     PEQFDDLFFSTSVEAECRRADRRGNAGATAQLFAELLDADHDRASDLASRHPRDLSAG
                     ERLCLALAIQLTARPRVLLIDEPSRGLDAAARALVGRAIVAAATRGAAVLMATHDEDF
                     AATYATRTIGMAAGRIEPVAVTS"
     gene            complement(8611..9123)
                     /locus_tag="PLW15_05155"
     CDS             complement(8611..9123)
                     /locus_tag="PLW15_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33822.1"
                     /translation="MTRVSRTAPLLAAIVALALAGCAAPATAPGSTPEPQSTAAGDCS
                     QVAVVVDFGPLDAPAIADCVAAGAAADILSAAGVTTEGTVDYGDQVVCRVNGQPSADA
                     EVTIEGEAPFVETCQTLSAVAYWALWVKTSPDSEWEYAQEGVSTLELTDGQSVGLVYT
                     PGTETVPPQD"
     gene            complement(9120..>9700)
                     /locus_tag="PLW15_05160"
     CDS             complement(9120..>9700)
                     /locus_tag="PLW15_05160"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOT33823.1"
                     /translation="IHNVQAERSPFRLGEVPGFPAAKRLRSGQKVTIAVATFPNGHPR
                     SRSRSQDIDTLLAKQAAGANLAITQLFFHADEYLGFVDEARASGVTMRILPGIMPVLS
                     VTRLQRMLELSGEATPVDLLATLESAPDAAAASRLGIDACISLARDLLDGGADGIHLY
                     TFNSSAAPTAVLEGAGLIGHSTPLTASIEGTP"
BASE COUNT         1639 a         3312 c         3270 g         1379 t
ORIGIN      
        1 cgctcaagaa gagcctcgaa gaccgcgtgt ggatcggcca ctccaccgaa gagcacgaag
       61 ttggccgcgc tgggcagcgg cgtgtagccg agtgcgggca gggcgaggat catgcggtcg
      121 cgctggtgct tgatgtcctc caccatggcg agcatctccg gcgcgtgcgc gagggcggcg
      181 atcgcggccg cctgggtgag cgcggacagg tgatagggaa gccgtaccaa gcgcagggca
      241 tccgtcaccg cggggtcagc cgcgagatac cccagtcgcg cacccgcgaa ggcaaacgcc
      301 ttgctcatgg tgcgcgagat gagcaggcgg ggacgaccct cgagaagggt gagcgcggtg
      361 tcaccgtcgg cgaactccgc ataggcctcg tcgacgagca ggatgcccgg ggtcgcgtcg
      421 tacgcggcct cgatggtgtc cagcccgagt ggcgtgccgg tcgggttatt gggcgagcag
      481 aggaacacga tgtccggctg cttgcgttcg atcgccgcga caacggtctg cggcgagagc
      541 tcgtagccgt catcacgctc ggccgtgacc cagcgcgtgc cggtgccgct cgcgatgatc
      601 gagtgcatcg aataggtggg agggaagccg agcacgctac gaccgggtcc gccgaacgcc
      661 tgcagggtct gctgcagcac ctcgttggac ccgttggccg cccagatgtt gtcgcgagtg
      721 agtccgtgcc cgaggtagcc ggcaagggaa tcccgaagct cggtgaactc gcggtcggga
      781 taccgattga ctcccatgac cgcgccggcg agagccatga cgatgtcggt ggcaaccgcc
      841 tcgggaatcg cgtgagtgtt ctcattgacg ttgagctgca cccgaacgtg cttctgcggt
      901 gcgccgtagg gctcgagacc acgaaggtcg tcgcgaatcg gcaggtcgtc gaggcttgtc
      961 acccgtcaag cctaggacgt gggcgcaccc cgctcgcgcg actagtgagc gtaggcggcg
     1021 ggaatcgcga gctcctggcc cgcgtagacg tccgccgact cgaggaggtt gagcttcatg
     1081 atctcgccga tcacatcacg cgggtctgcc gccggggcga tgctctccgc gagctgccag
     1141 agcgtctgcc cggactcgac cgtcacatac tcgaagccga ccgaggatcc ctcgagcgac
     1201 gcggtcgctc ccccgccgtt gagggcgaag acgagtgccg cgatgacgag tggtgtggcc
     1261 gcgaggaacg tgagaaccgc gcggccacgc ttggtcaggc gcagcttcgg ggcggaggcg
     1321 ggcactgact gtgccgtgcg ggccggggtg aatccgtagc ccggtgcgat gactgctgtg
     1381 ctcatcggtc gtcctcttcc tgtcgatcgt gctgcctgtc gggggtttcg tatccggcgc
     1441 tcggccggga gagccggata cgaagtgtgg ggggaggtgc gtgtcgtacc tgtgttccga
     1501 acatacattc gattgcctcc cttttcaaga ggatcttcga acaagccacc gacattgcct
     1561 gcaacacact cgaacaggtg tttgttttgt gccttcggaa cggatacagt ttcgaatgag
     1621 ccaccgacat tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatggagg
     1741 tcctcatcga cctgccgtct ctcgagggcg cggatgccac ggccgagagc acctcgccgg
     1801 cggtgggtga cgccgtgatg gtgccgctcg tgggtcgcat cgcggcgggc atcccgatca
     1861 ccgccgacca gcaggtggac gaggtgttcc cgctcccccg ccagctgacc ggcaagggcg
     1921 agctgttcat gctcaaggtc gtgggcgagt cgatgatcga cgccgccatc tgcgacggcg
     1981 actgggtcgt cgtgcgccag cagcagaccg cggagaacgg cgacatcgtg gccgccatgc
     2041 tggacagcga ggccaccgtc aaggtgtttc gccagcgcga cggtcacacc tggttgctgc
     2101 cgcgcaactc caacttcgag ccgatcctcg gcgatcacgc cgtcgtgctc ggcaaggtcg
     2161 tcacggtact gcgcgcgctg tagggcgtca cacaggacag tgctccacag cggacagcta
     2221 ggctgatccg gtggcatcga gtgtgacttc cccgtcgatc gccgacctct cggacattcc
     2281 ccggccggcg cgctccgacg cgctgatgga gaccctcagg cagcgtgtcg tcatcgccga
     2341 cggtgcgatg ggcacgatgc tgcaggatgc cggtcccaca atggaggact tccagcagca
     2401 cgaggggtgc aacgagatcc tcaacgtgtc gcgccccgac atcgtcgaga cgatccacca
     2461 cgcctacctc gacacgggca tcgacgccat cgagacgaac acgttcggat cgaactggtc
     2521 gaacctctcc gactacggca tcgaggaccg catcgaggag ctctcccggg cgggcgccga
     2581 gatcgcacgg cgggccgttg agacctacga ggcccgagat ggccggatgc gctgggtgct
     2641 cggctcgatc ggccccggca ccaagctgcc cagcctcggc cacaccacct acgacaacct
     2701 caagctgacc ttcggcgagc aggccaaggg actcatcctc ggtggctccg acgcgctcat
     2761 gatcgagacc tcgcaggatc tgctgcagac gaaggcggcg gtcaacggct gcaagctcgc
     2821 gatggtcgag acgggcatcc ggctacccat cttcgtcgag gtgaccgtcg aaaccacggg
     2881 caccatgctc atgggtagcg agatcgcggc agccctgacg actctcgagc ccctcggcat
     2941 cgacatgatc ggcctgaact gcgcgaccgg tccggacgag atgagcgagc acctgcgcca
     3001 tctgtcgaag ttcgcccaca tcccggtgat gtgcatgccc aacgcgggcc tgcccgtgct
     3061 cggacccaac ggtgccagct acccgctcac ccccgacgag ctggcgacgg cgcacgagca
     3121 gttcgtgaag gagttcgggc tgggcctcgt gggcggatgc tgcggcacga cgcccgagca
     3181 cctcaaggcc gtcgtcgatc gcatcggcgg cgcacgcgcc ctcacgcgaa cgatcgagga
     3241 ggacaacggc gtcgcgagtc tgtaccagca cgtgccgttc acccaggact cgtcgtatct
     3301 cgcgatcggt gagcgcacca acgcgaacgg ctcgctcgcg ttccgtgcgg cgatgctcga
     3361 ggagcgctgg gatgactgcg tcgacatcgc ccgaagccag gtgcgcgagg gcgccaacct
     3421 gctcgacgtc tgcatcgact acgtgggccg cgacggagtc gccgacatgc gcgagatcgt
     3481 gtcgcggctc gccagcgcct ccaccctgcc cctcgtcgtc gactcgaccg aacctgccgt
     3541 gctgcaggcc ggaatggagc tcatcggcgg tcggccggtg gtcaactcgg tgaacttcga
     3601 ggatggcgac ggcccgcagt cgcggttcgg ccgcatcatg ccgctcgtca aggagcacgg
     3661 cgccgccgtg atcgccctca ccatcgacga ggagggccag gcccgcacgg ccgagcacaa
     3721 ggtgcgcatc gcgtcccggc tcgtcgactc cctcgtgggc gagtggggca tgcgggtcga
     3781 cgacatcatc gtggatgctc tgaccttccc catcgcgacg gggcaggagg agacccgacg
     3841 cgacgcgatc gagacgatcg aggcgatccg gcagatcaac gccaagtatc cgggcatcca
     3901 caccacgctc ggtgtctcga atgtctcgtt cggcctgaac cctgctgccc gatccgtgct
     3961 caactccgtc ttcctgcacg aggcggtcga ggccgggctg gactcggcca tcatcaatgc
     4021 ggcgaagatc gtgccgctgg catccgtgcc cgacgatcgg cgcaaggtcg cgctcgacct
     4081 ggtctgggac aagcgcgagt acgacgccga cggcaccgtc acctatgacc cgctgtccgc
     4141 catgctcgac ctgttcgacg gagtcgactc agcggcgctc aaggaccagc gtgccgccga
     4201 actggccgcg ctgccggtgg gacagcggct cgagcgccgc atcatcgacg gcgaggcgaa
     4261 gggcctcgag gcagacctcg acctcgcccg cgccgacggc atgagtgcgc tgtcgatcat
     4321 caacgaccac ctcctcgctg gcatgcaggt cgtcggccag cgcttcggca gcggcgagat
     4381 gcaactgccg ttcgtgctcc agtctgccga ggtgatgaag gcggccgtcg ccctcctcga
     4441 accccacatg gagaagtcgg atgccgcggg caagggcacc atggtcatcg gcacggtgcg
     4501 cggggatgtg cacgacatcg gcaagaacct cgtcgacatc atcctcacca acaacggcta
     4561 cgacgtcatc aacatcggca tcaagcagcc gctcagcgag ttcatccgcg ccgccgagga
     4621 gcacaacgcc gacgtgatcg gcatgagcgg cctgctcgtg aagtcgacgg tcgtcatgaa
     4681 ggagaacctc caggagctct cggcgcgcgg tctcggcacc cgctggccga ttctgctggg
     4741 cggtgcggcg ctgactcgca cgtacgtgga ggacgacctc gcgtcgatct tcgacggcga
     4801 ggtgcgctac gccagagacg ccttcgaggg cctcgccctc atggagccac tcgtggcgat
     4861 cgctcgcgga gccgcacgcg actcagtcgg cctcccgccg ctgaagaagc gcatccacgc
     4921 ccgcacgacc atcgacgtga ccgagcccga gaacatgccg cagcgatcgg atgtcgtcac
     4981 cgacaacccg atccccgccc cgccgttctg gggcacccgc atcgtcaggg gcatcgcgct
     5041 tgccgactac gcggccttcc tcgacgaacg cgccaccttc atgggtcagt ggggcctcaa
     5101 accgagccgc gccgaggacg gcgcgagcta cgaggaactc gtgcagaccg agggccggcc
     5161 gcgcctgcgc tactggctgg atcgcatcct cgccgagggc atgatcgacg cctcggtcga
     5221 ctacgggtac ttccccgcgt actcggaggg cgacgacctg gtcatcctgc accacggcga
     5281 cgacccgacc ggcatcctcg gcaccccggg actgctcgcc ccggatggcg gctcgggcgg
     5341 gccgatcggc accgaacgcc tgcgcttcag cttcccgcgc cagcggcgcg accgccacct
     5401 ctgtctggcc gactacgtgc gctcgcgtga gtcagggcag gtcgacgtgg tcgccctgca
     5461 actcgtgacg gccggctcgg ccgtcgacgc cttcaccgcc cagatgttcg ccgagaacaa
     5521 gtaccgcgac tacctggaac tcaacggact cacgatgcag ctcaccgagg cgctcgcgga
     5581 gtactggcat tcgcgcgtgc gcgccgaact cggcttcgcc gctgaggatc cgaccgacat
     5641 cgccgggatg ttcaagctca actaccgcgg agcgcgcttc tccctcggct acccggcctg
     5701 ccccgacatg gaggatcgcc gcaaggtggt ggagctgctc aagcccgagc ggatgggcgt
     5761 ggaactgtct gaggagcttc agcttcatcc cgagcagtcg actgacgcgt tcgtgttcca
     5821 tcaccccgag gcacggtact tctcggtata gccccggatg ctcgcctggt gcggcggccg
     5881 cctatgcgcg cccgccgcag cgccgccagc gccgcgggac caaccagaac gatgccgacc
     5941 accgtcgtca ccgcgcgcac ggtgtcccag gtcagcgtgg atgtcacgag ggagtacagg
     6001 ccgaacgagg cgagattctg gccgaggggc gcgccggcgg aatacgagat gtccgacccg
     6061 gcacccacgg cgaacggcca gaaccacagg ttcatgatga agccgaaggc atacgaggcg
     6121 aggatgccgt agcccaccag catgagcacc tccagccgcg ctgccctgcg ctgcgatcgc
     6181 gcggtgatgc gcggcagcag gcccgcgccg gcggcgaccc agccgcacgc gaagatctgg
     6241 aagggcgtcc acggtccgat gccgccccag agcaccgacg acagggagat caccagcatc
     6301 ccgagcagga agccgaaccg cgcaccgaag gcccggcccg cgaggatgag gacgatgaac
     6361 acggcctcga gcccgccgac accggtgccg gcgatccgca cggcggcacc gacggcggtg
     6421 agcatcccca gcagcgccac tgtggttgcg ctgtgcatct ggcggtcaac ggtcagcgcc
     6481 agcgcgacaa ccgcgacagg cactagggcc agcgcgagca ccggcacagc cgcctgggcg
     6541 tccctcggca ccgcggttgc gatgagcggc cagaggaacc cggcgagggc taacaagctg
     6601 gcgatgagga gggtcactcg cgagctgact tcgcgacgcg cgccgaccac ggcaacgact
     6661 cggtatttca cgaggtcacc gctacaggct cgatgcggcc ggccgccatc ccgatcgtgc
     6721 gcgtcgcgta ggtcgcggcg aagtcctcgt cgtgggtcgc catcagtacg gcggctcccc
     6781 gggtcgcggc cgcgactatc gcgcggccga cgagggcgcg agccgcggca tccagcccgc
     6841 ggctcggctc gtcgatcagg agcacgcgag ggcgagccgt caactggatc gcgagcgcga
     6901 ggcagagccg ctcccccgcc gacaggtcgc gggggtgccg cgacgccaga tcactcgcgc
     6961 gatcgtgatc ggcgtcgaga agttcggcga agagctgcgc tgttgcgccg gcgttgccgc
     7021 gtcggtcggc tcgacggcac tccgcctcga cggaggtgga gaagaacaga tcgtcgaact
     7081 gctcgggcac cagggcgaca tagctcgggc gttcgcgccg gcccacggcg tggacatcac
     7141 acccatcgac gaagacggcg ccgcgctggg tgggcagggc agtggcgagc agcagactgc
     7201 tcttgcccgc cccgttggga ccgcggagag cgaccacctc accaccgcac aggtgcaggc
     7261 tggcgccggc cacggcgacc tcgtcaccgt gggtgactgt caggtcccgg atctcggcga
     7321 tgggcgccgc gctgacgggc gcaggcccga ggtcggcgtg gcgcggctgc gtccgtgccg
     7381 aatcacgcag tgcgagctca gcaccggttc gtcggatgac gctgccgtcg ctcacctcga
     7441 gccagacatc cgctcggtcg ccccagcccc ggatggcatg ctccgcaacc acgacggcga
     7501 ctccggcctg gtgggcgagc tcatcgagaa ggtcgatgac catcacccgt gccgagtcat
     7561 ccagatcggc cagtggctca tcgacgagca gcagaaccgg atgctcgatg agagcggcac
     7621 cgatcgccac caaacacgcc tccccggcag acagggccac cgtcgagcgc gtgagcagat
     7681 gctggatgcc cagccgccgc gccgtctcct ccaccctcgc agtgacgatg acggcggcca
     7741 cgtcccggtt ggccagcgcg aagccgagtt cctcccgcac ggtcgccgcg acgaacgaca
     7801 gtcgaaccga ctgcgctaca acccccacga gccccgcggt ctcgcgcgga gacaccaggg
     7861 cgcggttcac accggccacc tcgacgatcc cctgctgctc tcccccggcc acgtgctgga
     7921 acagcccgct gagcgcgtcg agaagagtcg acttgcccga accggtggcg cccgtcacga
     7981 cggtgagggt gccgggcgag acgtcgaggt cgagatcgac gaggctccac gatccgtcgt
     8041 acccgagact ggcactgcgc accgacgccg gccgctcaca ctccggctcc gcccggcgtg
     8101 tggcggcaaa gccacgcacc tccatggccg atcccagagc gatggctcgc tccacgctct
     8161 gctccagcac gggcaccaga agggatgcca ctccccgctg accgcggagg cggcgggcag
     8221 tgcgcactcg cgccaccgag cccaccaacg ccggcaccgc accccacgcc acgactagcg
     8281 cgcgcgagat cgtgcgcacg ggcccgcgta ctgcgccacg cgcggccagg gcgcgcagat
     8341 cgaacaccac cgcgagcagg ccgaacgcga gcaccaggag ggcgaaggga acggcgctcc
     8401 aggcgatcag ggcgacaccg cccgaggtca ccgggccgag cagggtgatg tgctcgaagg
     8461 gacccgacag ccgcacccgg ggcaggtcga gcagcagcat ccccgggccg tcggcgccac
     8521 cgaacacgat gcggtacagc acccggacca cgacgaaccc gaccgcgacc aggatcgcgg
     8581 tccgcagagc ggcaagccgg gtcagacgca tcagtcctgc ggcggtacgg tctcggtgcc
     8641 gggcgtgtag accaggccga ccgactgtcc gtcggtcagt tcgagcgtgc tgacaccctc
     8701 ctgggcgtac tcccactcgc tgtcggggct ggtcttgacc cagagtgccc agtacgcgac
     8761 ggcactcagt gtctggcagg tctccacgaa cggtgcctcg ccctcgatgg tgacctccgc
     8821 gtccgctgag ggctggccat tcacccggca gaccacctgg tccccgtagt cgacagtgcc
     8881 ctcggtcgtc acgccggccg cgctgaggat gtcggcggcg gcccccgcgg cgacgcagtc
     8941 ggcgatcgcc ggcgcgtcca acggcccgaa gtcgaccacg acggcgacct ggctgcagtc
     9001 accggccgcc gtgctctgcg gctcgggagt ggagcccggg gccgttgcgg gcgccgcaca
     9061 cccggcaagg gcgagggcca cgatcgcggc gagcagaggg gcagtacggg agacgcgggt
     9121 cacggagttc cttcgatcga ggctgtcaga ggggtcgagt ggccgatgag gccggcacct
     9181 tcgagcacgg cggtcggagc cgccgagctg ttgaaggtgt acaggtggat gccgtcggca
     9241 cctccgtcga ggaggtcgcg ggcgaggctg atgcacgcat cgattccgag acgactcgcg
     9301 gccgcggcat ccggtgccga ctcgagggtg gcgagcagat cgacaggcgt cgcctcgccc
     9361 gacagctcaa gcattcgttg aagccgggtg acgctcaaca ccggcatgat cccgggcagg
     9421 atgcgcatgg tcaccccgga cgcgcgtgcc tcgtcgacga acccgaggta ttcgtcagcg
     9481 tggaagaaca gttgcgtgat cgccaggttg gcacccgcgg cctgcttggc gagcagggtg
     9541 tcgatgtcct gtgatcgcga tcgtgaccgt ggatgcccgt tggggaacgt cgcaacggcg
     9601 atcgtgacct tctgcccact gcgcagtcgc ttggcggcgg ggaatccggg cacctcgccg
     9661 agccggaacg ggctgcgctc ggcctgcaca ttgtggatca
//
LOCUS       DAOSOU010000065         9403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7265_length_9403_cov_6.661960, whole genome shotgun sequence.
ACCESSION   DAOSOU010000065 DAOSOU010000000
VERSION     DAOSOU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7265_length_9403_cov_6.661960"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLW15_05165"
     CDS             complement(<1..777)
                     /locus_tag="PLW15_05165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT33824.1"
                     /translation="MSTAQSSDRSATIPSRPEAAVQRRLPGLDGLRAIAVIVVIAFHF
                     VPAAAPGGYLGVDVFFVISGFLITTLLLRERSRTGRVSVPRFWLRRARRLLPALAVIV
                     LTCGAAAAVIGGDVLVHLGSQVLGAATFSSNWLYIVQGTSYFATTSPELFRNLWSLAV
                     EEQFYLLWPGLLILLLFVRWRWLRVVIVSLLAVSSAVAMALLFTPPGDATRVYYGTDT
                     HSFGLALGAAVAIAIEGRMPARWLSRTLALIGAAAAGALVA"
     gene            812..1690
                     /gene="truA"
                     /locus_tag="PLW15_05170"
     CDS             812..1690
                     /gene="truA"
                     /locus_tag="PLW15_05170"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT33825.1"
                     /translation="MSDEPSDEDRTRLRLDIAYDGSGFTGWAKQPGIRTVQGELETAL
                     ATVFSRHGEPPVLTVAGRTDAGVHATGQVAHLDLGPAQLSHLRIPHGKGPVEPTAVLA
                     KRLNGIAGLRSDVYVSRVSIAPPGFDARFSPIWRRYEYRIADARAERNPLLRGHTLWY
                     PAALDEQRMADAANELLGLHDWAAYCRPREGATTIRELQAFSWDRDDTGVLIARLQAD
                     AFCHSMVRALVGAAVSVGEGKLVGDRLTGIRDEAQRTSEFKVLPAHGLTLVEVGYPDD
                     AALAARAEQTRARRER"
     gene            1904..2350
                     /gene="rplM"
                     /locus_tag="PLW15_05175"
     CDS             1904..2350
                     /gene="rplM"
                     /locus_tag="PLW15_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT33826.1"
                     /translation="MTRTFSPTPADVQRDWVIIDATDIVLGRLASHAATLLRGKHKPT
                     FAPHMDMGDFVIIINAEKVALTGSKLAQKKAYRHSGYPGGLTATTYSEMLEKHPTRAV
                     EKAIRGMLPKNSIGRAQLTKLKVYAGAEHPHAAQQPKPYTLSQVAQ"
     gene            2388..2891
                     /gene="rpsI"
                     /locus_tag="PLW15_05180"
     CDS             2388..2891
                     /gene="rpsI"
                     /locus_tag="PLW15_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT33827.1"
                     /translation="MAKIEDSLTGDVVAEDVLESYTTETPADTTPKAPRAILNVPGAA
                     VGRRKEAIARVRIVPGTGKAVVNGRELAEYFPNKLHQQLINDPFKILDLLGAYDVTAK
                     ITGGGPSGQAGALRLAIARSLNEIDRENNRAALKKAGFLTRDARVIERKKAGLKKARK
                     ASQFSKR"
     gene            2921..4282
                     /gene="glmM"
                     /locus_tag="PLW15_05185"
     CDS             2921..4282
                     /gene="glmM"
                     /locus_tag="PLW15_05185"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT33828.1"
                     /translation="MPRLFGTDGVRGLANSDLTVELALGLAQSAAVVLGKGRVADGRR
                     ASGRRPIAVIGRDPRISGEFLASAVASGLASSGVDVFDAGVIPTPGLAFLVGDFKADL
                     GVMISASHNPAPDNGIKFFATGGTKLPDDVEDRIEAALHEPKLAPTGGNVGHIRRFSD
                     AEDRYLVHLLSTLPNRLDGVHVVIDCAHGAASGVSPQVFTDAGATVTVIGADPDGLNI
                     NDGVGSTHLDNLATAVLEHGADVGIAHDGDADRCLAVDAAGNVVDGDQIMAILAVSMS
                     ERGVLTDNTLVATVMSNLGLRRAMAEHGIRMVETAVGDRYVLEALGEQGLSLGGEQSG
                     HVIMTKYATTGDGILTGLHLVAEMARTGKSLAELASVMTVYPQVLVNVRGVDHHALRD
                     DVTIADAVTVAETALGDTGRVLLRPSGTEPMVRVMVEAEHQDTAQRIAQELADVVREQ
                     LAL"
     gene            complement(4310..5236)
                     /locus_tag="PLW15_05190"
     CDS             complement(4310..5236)
                     /locus_tag="PLW15_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOT33829.1"
                     /translation="MSDAATADSISVLDVERKVFRASIGLGIGLAASALVLGLVSGTR
                     VLVFDGAFGSIGILVSWVSLRVSAAVAQGPTKRFPFGLQALTPLIVVVQGVASAATIL
                     YAAFDAVIVITNGGKPVDAGLVAAYSAASTIASFIFVWWINRHARVSDLVGAEAIAWR
                     SGAIRGLVMTIGALAAVVLAALSFTVILDYIDPILVLVSCALVAPLPWRLLRYGVLEL
                     LEAAPEPTIAARIDEVITEVGGRFGLGESVVRSAKLGGRLYVEVTFLVGAGTWSVDDE
                     DSVRRAIIEGLRSTGLDLWATIEITADPELLD"
     gene            complement(5269..6216)
                     /gene="coaA"
                     /locus_tag="PLW15_05195"
     CDS             complement(5269..6216)
                     /gene="coaA"
                     /locus_tag="PLW15_05195"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HOT33830.1"
                     /translation="MSDSHQHNGQLSPFIELDRSDWAALAPTMTAPLTQTELVELRGL
                     GDTLDLQEVEEVYLPLSRLLNLYAEGAQQLHRATTTFLGASSARTPFVIGIAGSVAVG
                     KSTVSRLLRELMSRWEGTPRVDLVTTDGFLFPNAELERRGIMDRKGFPESYDRRSLLR
                     FVSAVKSGAREVHAPFYSHLAYDIVADAEITVSAPDVLIIEGLNVLQPPAPGRRLAVS
                     DLFDFSIYVDARTQDIAHWYEERFLSLQRGAFANPLSYFHRFASLSEAEARARASEIW
                     KAINEPNLVQNILPTRPRASLVLRKNSHHAVSSVLLRKT"
     gene            6271..8124
                     /gene="glmS"
                     /locus_tag="PLW15_05200"
     CDS             6271..8124
                     /gene="glmS"
                     /locus_tag="PLW15_05200"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOT33831.1"
                     /translation="MCGIVGYVGDNKSIEVLLGGLRRLEYRGYDSAGVAVIDSEGHIG
                     TRKRAGKLQVLVDDLEDRPLANGATGIGHTRWATHGGPTDRNAHPHLSADGKLALIHN
                     GIIENFAELKQELVESGATFTSETDTEVAALLVGREYESTQDLTEALRRVVARLHGAF
                     TLLVMHEDQPGVVVGARRNSPLVIGLGDGENFLGSDVAAFVEFTRRAVAIGQDQIVTI
                     RPDSVEVIDFGGAPVETEEFDVAWDASASEKGGWSSFMAKEISEEPEAVGNTILGRVH
                     DGLVKLAELDPIGDAALADLDRIVIIACGTAAYAGMLGKYAIEAWARVPVDVELSHEF
                     RYRDPILTERTLVISISQSGETMDTLVAVQHATAAGARVLSVCNTQGATIPRESQAVV
                     YTHAGPEVAVASTKAFVAQVTALYLIGLHLAGVRGTLTDDQIRENVVELQAIPDKIAE
                     VLSVSQAEIGQLAHWMTDTQSVLFLGRNVGYPVALEGALKLKELAYIHAEGFAAGELK
                     HGPIALIEPGQAVIVVVPSPRDPNSLHPKVISNIQEIRARGARVIAIAEKGDAAVLPF
                     ADEVIPIPLAGSLFEPLLAVVPLHIFGMELASAKGLDVDQPRNLAKSVTVE"
     gene            8128..8493
                     /locus_tag="PLW15_05205"
     CDS             8128..8493
                     /locus_tag="PLW15_05205"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT33832.1"
                     /translation="MIVGVGVDVVDLARFERALQRTPALISRLFAESEQLKDGQPMPL
                     RSLAGRFAAKEALIKALGDSTGVQWHDMRVVNDSLGNPSFELHNSTRAIADGRGITAI
                     HLSMAHDAGIAIAYVVAES"
     gene            8490..>9403
                     /gene="alr"
                     /locus_tag="PLW15_05210"
     CDS             8490..>9403
                     /gene="alr"
                     /locus_tag="PLW15_05210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT33833.1"
                     /translation="MTREALVDLDAIRSNLAVLSTATAATPAMVIVKANAYGHGAVPV
                     ARAVVEAGAAWLGTADLAEALELRAAGVTAPLLAWLHEPDVDFAPAIEGGVDIGVSYP
                     QQLERVAAASGRAFVQLKVDSGLGRNGADATQWLELVEAAAAHQRAGRLVVRGVWSHL
                     ANAGPEADAAQIATFTEALEAARAAGLEPELVHLAATAGALRVPASRFGMVRLGIGVY
                     GLSPDDTPVPGLTPAMTLSAPVVAVKHVPANHGVSYGFDYRTTAPTTLALVPVGYADG
                     LPRSASGGAPVAINGRTYRIAGRIAMDQV"
BASE COUNT         1592 a         3240 c         3162 g         1409 t
ORIGIN      
        1 ggcgacgagt gcgcccgcgg cggcggcgcc gatcagcgcg agggtgcgcg atagccagcg
       61 ggcgggcatc cggccctcga tggcgatggc gactgccgca ccgagagcca acccgaagct
      121 gtgggtgtcg gtgccgtagt agacgcgggt ggcatcaccc ggcggcgtga acagcagcgc
      181 catcgccacg gccgacgaga cggcgagcag cgacacgatg accactcgga gccagcgcca
      241 gcgaacgaac agcagcagga tcagcagccc gggccacaga aggtagaact gctcctcgac
      301 ggccagtgac cacaggttgc ggaacagttc ggggctggtg gtcgcgaagt agctcgtgcc
      361 ctgcacgatg tagagccagt tgctgctgaa ggtcgcggcc ccgagcacct gcgatccgag
      421 gtgcacgagc acatctccgc cgatgacggc ggcggccgcc ccgcaggtca gcacgatcac
      481 cgcgagggcg gggaggagcc gacgggcgcg gcgcagccag aaccggggga cggacacgcg
      541 accggtgcga gagcgctcgc ggagcagcag agtggtgatg aggaagccgc tgatgacgaa
      601 gaagacgtcc acgccgagat agccgccggg ggcggccgcc ggcacgaagt ggaaggcgat
      661 gaccacgatc acggcgatcg cccgcagccc gtcgagaccg gggagcctac gctgaaccgc
      721 cgcttccggg cgactcggaa tcgtggcgct gcgatcggaa ctctgtgcgg tgctcatggc
      781 tcatccgtca tcaagcctag acttggcagc cgtgagcgac gagccgagcg acgaggatcg
      841 cacccgcctg cgcctcgata tcgcctacga cgggagcggg ttcacgggct gggcgaagca
      901 gcccggcatc cgaaccgtgc agggcgagct cgagaccgcg ctcgccactg tcttcagtcg
      961 tcacggcgag cccccggtgc tcacggtcgc cggccgcacc gacgccgggg tgcacgccac
     1021 cggtcaggtc gcgcacctcg atctcggccc ggctcagctc tcccacctgc gaatcccgca
     1081 cggcaagggc ccggttgagc ccacggcggt gctggccaag cggctcaacg gcatcgcggg
     1141 gttgaggtcg gacgtctacg tgtcgcgcgt gagcatcgca cccccggggt tcgacgcgcg
     1201 gttctctccg atctggcgcc gctacgagta ccgcatcgcg gatgcccgtg ccgagcgcaa
     1261 cccgctgctg cgcggccaca cgctctggta cccggccgcg ctcgacgaac agcgcatggc
     1321 ggatgccgcc aacgagctgc tcgggctgca cgactgggca gcgtactgcc ggccacggga
     1381 gggtgcgacc acgatccggg agctccaggc cttcagctgg gatcgcgacg acacgggcgt
     1441 gctcatcgcg cgccttcagg ctgacgcgtt ctgccacagc atggtgcggg cgctggtcgg
     1501 cgctgctgtg tcggtcggtg agggcaagct ggtgggcgac cggctcaccg gcatccgcga
     1561 cgaagcccag cgcaccagcg aattcaaggt gttgccagcc cacgggctga cgctcgtcga
     1621 ggtcggctac ccggatgacg cggcgctcgc cgctcgcgcc gagcagaccc gcgctcgccg
     1681 ggagcggtga tcgagccccg ggagcggtga tcgagctccg ggatcggtga tcgagctcgt
     1741 cgagatccgt ttgacgggca tccacgggtc gactagtctt acgaggttgt gttcgccacg
     1801 agtgcgacgc acggttgagc cctccaccgt ttgtgttctg gatgacagaa ccggcaccgg
     1861 agcgggattc acgaactcct cgacaaaaga aagcagtact tctgtgacgc gtacattctc
     1921 gcccactccc gctgacgttc agcgtgactg ggtcatcatc gacgcgaccg acatcgttct
     1981 cggtcgtctg gcgagccacg ccgcaacgct cctgcgcggc aagcacaagc cgacctttgc
     2041 tcctcacatg gacatgggcg acttcgtcat catcatcaac gccgagaagg tggccctcac
     2101 cggctccaag ctcgcgcaga agaaggccta ccgccactcg ggttacccgg gcggcctgac
     2161 ggcgaccacc tactcggaga tgctggagaa gcaccccacc cgcgcggtcg agaaggccat
     2221 ccgcggcatg cttccgaaga actcgatcgg ccgggcccag ctgaccaagc tcaaggtcta
     2281 cgccggtgcc gagcacccgc acgctgcaca gcagccgaag ccctacaccc tctcccaggt
     2341 cgcccagtag ccccggctgc tgaagaaaca caaaaggact tactacattg gccaagatcg
     2401 aagattccct caccggcgac gtcgtcgctg aggacgtgct cgagagctac accaccgaga
     2461 ctcccgctga cacgacgccc aaggcgccgc gtgccatcct caacgttccc ggcgctgccg
     2521 tgggccgtcg caaggaggcg atcgcccgcg tgcgcatcgt ccccggaacc ggcaaggcgg
     2581 tcgtcaacgg ccgtgagctc gccgagtact tccccaacaa gctgcaccag cagctcatca
     2641 acgacccgtt caagatcctc gatctgctcg gtgcgtacga cgtgaccgcg aagatcaccg
     2701 gcggtggccc ctccggccag gccggcgcac tgcgcctcgc catcgctcgc tcgctcaacg
     2761 agatcgaccg cgagaacaac cgcgccgccc tcaagaaggc gggcttcctc actcgtgacg
     2821 cccgcgtcat cgagcgcaag aaggccggtc tcaagaaggc tcgcaaggcg tcgcagttct
     2881 cgaagcgcta gtcactactg ctctgtagcg gctacctcag atgccacgac ttttcggcac
     2941 ggacggagtt cgcggactcg cgaactccga cctgaccgtc gagcttgccc tgggcctcgc
     3001 ccagtccgca gccgttgtgc tcggcaaggg gcgagtcgcc gatggtcgtc gtgcctcggg
     3061 acggcgaccg atcgccgtca ttggtcgcga cccgcgcatc tcgggggagt ttctggcatc
     3121 cgctgttgcc tcggggctcg cgagctcagg cgttgacgtc ttcgacgccg gcgtcatccc
     3181 cacaccggga ctcgccttcc tcgtcggcga cttcaaggcc gacctcgggg tcatgatctc
     3241 ggcgagccac aacccggcgc ccgacaacgg catcaagttc ttcgcaacgg gtggcaccaa
     3301 gctccccgac gacgtcgaag accgcatcga ggcggcgctg cacgagccca agctcgcgcc
     3361 caccgggggt aacgtcggcc acatccggcg cttctccgac gccgaggacc gctacctcgt
     3421 gcacctgctc tcgaccctgc cgaatcgcct cgacggcgtt cacgtcgtca tcgactgcgc
     3481 gcacggagct gcttccggtg tctcgcccca ggtgttcacg gatgccggcg ccacggtcac
     3541 tgtgatcggc gccgaccccg acggtctcaa catcaacgac ggggttggat cgacccatct
     3601 cgacaacctc gcgaccgccg tgctcgagca cggtgccgac gtgggcatcg cccacgacgg
     3661 tgacgccgat cgctgccttg cggtcgacgc ggcaggcaat gtcgtcgatg gcgaccagat
     3721 catggcgatt ctcgcggtgt cgatgtccga gcgcggcgtg ctcaccgaca acacgctggt
     3781 ggcgaccgtg atgagcaacc tcgggctgcg ccgcgcgatg gcggagcacg gcatccgcat
     3841 ggtcgaaacg gcagtcggcg accgctacgt gctggaggcg ctcggcgagc agggtctgtc
     3901 cctgggcggg gagcagtcgg ggcacgtcat catgaccaag tacgcgacca cgggtgacgg
     3961 catcctgacc ggcctgcacc tcgtggcgga gatggcccgc acgggcaagt cgctggcgga
     4021 gctcgcgagc gtgatgaccg tgtaccccca ggtgctcgtg aacgttcggg gggtcgacca
     4081 ccacgccctc cgtgacgacg tgaccatcgc cgatgccgtc accgttgccg agaccgctct
     4141 cggggacacc ggtcgggtgc tgctgcgccc gtcgggaact gagcccatgg tgcgggtcat
     4201 ggtcgaggcc gagcatcagg acacggccca gcgcatcgcc caggagctcg ccgacgtcgt
     4261 acgcgagcaa ctcgccctct agccgttcgc gccgaggccg gcagtacacc tagtcgagca
     4321 gctccgggtc ggcggtgatc tcgatcgtcg cccacaggtc gaggccggtg gagcgcagac
     4381 cctcgatgat cgcgcggcgc acgctgtcct cgtcgtcgac gctccaggtg cccgccccga
     4441 cgaggaacgt cacctcgacg tagagccggc caccgagctt cgcggagcga acgacactct
     4501 cgcccagccc gaatcgcccg ccgacctccg tgatcacctc gtcgatcctc gcggcgatcg
     4561 tcggctcggg ggcggcctcg agaagctcga gcacgccgta gcgcagcagc cgccagggca
     4621 ggggcgcgac gagcgcgcag gagacgagca cgaggatggg gtcgatgtag tcgaggatca
     4681 ccgtgaatga cagtgccgcg agcaccacgg ctgccagtgc accgatagtc atgaccaggc
     4741 ctcggatggc ccccgaccgc cacgcgattg cctcggcacc gacgaggtcc gatacgcgcg
     4801 cgtggcggtt gatccaccag acgaagatga aactggcgat cgtggatgcc gcggagtagg
     4861 cggcgaccag cccggcatcc accggcttgc caccgttggt gatcacgatc acggcgtcga
     4921 acgccgcgta gagaatggtc gcggccgagg cgaccccctg cacgaccacg atgagcggag
     4981 tcagtgcctg aagtccgaag gggaagcgct tggtcgggcc ttgggccacg gccgccgaga
     5041 ctctgagcga cacccacgac acgaggattc cgatgctgcc gaaggcaccg tcgaacacga
     5101 gaacccgcgt tcccgacact aggccgagca cgagcgcaga ggccgccagg cctatcccca
     5161 gaccgatcga tgcgcggaac accttgcgct cgacatcgag taccgagatc gagtccgccg
     5221 tggccgcgtc gctcatgtcg acacgctagc agtggcccga ccgggtgact aagtcttgcg
     5281 cagcagcacg ctcgacaccg cgtggtgaga gttcttgcgc agcaccagcg acgctcgggg
     5341 gcgggtcggc aggatgttct gcaccaggtt gggctcgttg atcgccttcc agatctcgga
     5401 tgcccgtgct cgagcctcgg cctcgctcag gctcgcgaac cggtggaagt aggacagcgg
     5461 gttggcgaac gcgccgcgct ggaggctgag gaagcgttcc tcgtaccagt gcgcgatgtc
     5521 ctgggtgcgg gcatccacat agatactgaa atcgaacagg tcgctgaccg cgaggcgtcg
     5581 gccgggggcg gggggctgaa gcacgttcag cccctcgatg atcagcacgt cgggggcgct
     5641 gacggtgatc tcggcgtcgg caacgatgtc gtacgccaga tgcgagtaga agggggcgtg
     5701 cacctcgcgg gccccgctct tcacggcact gacgaagcgc agcagggatc tacggtcgta
     5761 gctctccggg aagcccttgc gatccatgat gccgcggcgt tccagttcgg cgttcgggaa
     5821 caggaagcca tcggtggtga cgaggtcgac gcgcggggtg ccctcccagc ggctcatcag
     5881 ttcccggagc agtcgcgaca ccgtcgactt gccgacagcg accgagccag cgatgccgat
     5941 gacgaagggt gtgcgtgccg aactggcacc gagaaaagtg gtggtcgcgc gatgaagctg
     6001 ctgggctccc tcggcataca gattgagcag acgactgagc gggaggtaga cctcctcgac
     6061 ctcctgaagg tcgagcgtgt caccgagccc ccgcagttcg acgagctcgg tctgcgtcag
     6121 cggagcggtc atggtgggag ccagcgcggc ccagtcgctg cgatccagct cgatgaacgg
     6181 cgacagttgg ccgttgtgct ggtggctgtc tgacaccgtc cgatcctagc gtcgaggtgt
     6241 caaccagatt ggccgcctaa gatcgactcc atgtgtggaa tcgtgggcta cgtcggagac
     6301 aacaagagca tcgaggtcct tctgggcgga ctccgacgac tcgagtaccg cggatacgac
     6361 tccgcgggcg tcgccgtgat cgactccgag ggccacatcg gcacccgcaa gcgcgccgga
     6421 aagctccagg tgctcgtcga tgacctcgag gatcgcccgc tcgccaacgg tgcgaccggt
     6481 atcggtcaca cccgctgggc gacgcacggt ggaccgaccg accgcaacgc gcatccgcac
     6541 ctcagcgccg acggcaagct tgccctgatc cacaacggca tcatcgagaa cttcgcggag
     6601 ctcaagcagg agctcgtcga gtccggcgcc accttcacga gcgagaccga caccgaggtg
     6661 gctgccctgc tcgtgggccg cgagtacgag tccacgcagg acctcaccga ggccctgcgt
     6721 cgtgtcgtcg cgcgcctgca cggcgccttc acgctgctgg tgatgcacga ggaccagccc
     6781 ggcgtcgtcg tcggcgctcg ccgcaactcc ccgctggtga tcggcctggg cgacggcgag
     6841 aacttcctcg gttcggatgt cgcggcattc gtcgagttca cccgacgcgc cgtggcgatc
     6901 ggccaggacc agatcgtcac tattcgaccc gactccgttg aggtcatcga cttcggcgga
     6961 gccccggttg agaccgagga gttcgacgtc gcatgggatg cctcggcgag cgagaagggc
     7021 ggctggtcga gcttcatggc gaaggagatc agcgaggagc ccgaggcggt cggcaacacg
     7081 atcctcggtc gcgtgcacga cggactggtg aagctcgccg aactcgatcc gatcggggat
     7141 gccgcgctcg ccgacctcga ccgcatcgtg atcatcgcgt gcggcactgc cgcctatgcg
     7201 gggatgctcg gcaagtacgc catcgaggcg tgggctcgcg tgcccgtcga cgtcgagctg
     7261 tcgcacgagt tccgctaccg cgacccgatc ctcaccgagc gcacgctcgt gatctcgatc
     7321 agccagtcgg gcgagaccat ggacaccctc gtggccgtgc agcacgcgac tgcggcgggt
     7381 gccagggtgc tgagcgtctg caacactcag ggcgccacca ttccgcgtga gtcacaggcg
     7441 gtggtgtaca cgcacgccgg gcccgaggtg gccgtggcat ccacgaaggc cttcgtcgct
     7501 caggtgacgg cgctctacct gatcggtctg cacctggcgg gtgttcgcgg cactctgacg
     7561 gacgaccaga tccgcgagaa cgtggtcgag ctccaagcga tccccgacaa gattgcagag
     7621 gtgctcagcg tctcgcaggc ggagatcggt cagctggccc actggatgac cgacacccag
     7681 tccgtgctgt tcctcggccg caacgtcggc tatcccgtcg cgctcgaggg tgcgctcaag
     7741 ctcaaggagc tcgcgtacat ccacgccgag ggcttcgccg ccggtgagct gaagcacgga
     7801 ccgatcgccc tgatcgagcc gggccaggcc gtcatcgtcg tggttcccag cccgcgcgac
     7861 ccgaactcgc tgcacccgaa ggtgatctcg aacatccagg agatccgcgc tcgcggtgcc
     7921 cgagtgatcg cgatcgccga gaagggtgac gccgccgtgc ttcccttcgc cgacgaggtc
     7981 atccccatcc cgctcgcggg cagcctgttc gagccgctgc tggcggtggt gccgttgcac
     8041 atcttcggca tggagctggc atcggccaag ggcctcgatg tcgatcagcc gcgcaacctc
     8101 gccaagtccg tcaccgtcga gtaacccatg atcgtcgggg tcggcgtcga tgtcgtggat
     8161 ctcgctcggt tcgagcgggc acttcagcgc accccggcac tgatctcgcg cctgttcgct
     8221 gagagcgaac aactcaagga cggccagccc atgccgctgc gctccctcgc ggggcggttc
     8281 gccgccaagg aggcgctcat caaagccctc ggcgattcca cgggcgtgca gtggcacgac
     8341 atgcgcgtcg tcaacgacag cctcggcaac ccgtcattcg aactccacaa ctccacgaga
     8401 gcgatcgcag acggccgggg aatcaccgcc atccacctct cgatggccca cgacgccgga
     8461 atcgcgatcg cctatgtggt ggccgagtca tgacccgcga agccctcgtc gacctcgacg
     8521 cgatccggtc gaatctcgcc gtgctgtcca cggcgactgc ggccactccc gccatggtga
     8581 tcgtgaaggc caacgcctac ggacacggtg cggtgccggt ggctcgcgcg gtggtcgagg
     8641 ccggcgctgc ctggctgggc acggccgacc tcgccgaggc gctcgagttg cgcgcggcgg
     8701 gtgtcaccgc gccgctcctc gcctggctcc acgagcccga cgtcgacttc gcgcccgcga
     8761 tcgagggcgg cgtcgacatc ggcgtgagct acccgcagca gctcgagcgg gtggctgcgg
     8821 catccggtcg ggcgttcgtg cagctgaagg tcgactcggg cctgggccgt aacggagcgg
     8881 atgccacgca gtggctcgaa ctcgtcgagg ccgctgccgc gcaccagcgc gccggccggc
     8941 tcgtcgttcg cggcgtgtgg agccaccttg ccaacgccgg ccccgaagcg gatgccgcac
     9001 agatcgccac cttcaccgaa gcccttgagg cggcgcgcgc cgccgggctc gagcccgaac
     9061 tcgtgcacct ggccgcgacg gcgggagcgc tgcgcgtgcc ggcgtcccgg ttcgggatgg
     9121 tgcgtctcgg gatcggcgtg tacgggctgt cgccggatga caccccggtg cccgggctca
     9181 cgcccgcgat gacgctctcg gcgccggttg tcgccgtgaa gcacgtcccg gcgaatcacg
     9241 gggtctcgta cggcttcgac taccgcacga cggcgccgac cacgctggcc ctcgttcccg
     9301 tcgggtacgc cgacgggctg ccgcgctccg cgtccggcgg ggctccggtc gcgatcaacg
     9361 ggcgcaccta tcgcatcgcc ggccgcatcg cgatggacca ggt
//
LOCUS       DAOSOU010000066         9345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7313_length_9345_cov_5.645856, whole genome shotgun sequence.
ACCESSION   DAOSOU010000066 DAOSOU010000000
VERSION     DAOSOU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7313_length_9345_cov_5.645856"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1553)
                     /locus_tag="PLW15_05215"
     CDS             complement(126..1553)
                     /locus_tag="PLW15_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT33834.1"
                     /translation="MTMTEPSLTTYNEADLQQKAKDHLWMHFSRQSVMESGAGVPIIV
                     KGEGHHIWDSTGRKYIDGLSGLFVVNAGHGRKRLADVAARQAEELAFFPIWSYAHPNA
                     IELADRLASYAPGDLNRVFFSTGGGEAVETAFKLAKYYWKLQGRPTKHKVISRSIAYH
                     GTPQGALAITGIPGMKEMFEPLVPGGFRVPNTNFYRSEEMGFVASPGADSATAEREFG
                     LWAANRIEEMILFEGPETVAAVFLEPVQNSGGCFPPPPGYFQRVREICDQYDVLLVSD
                     EVICAFGRIGHMFACDEFGYVPDMITCAKGMTSGYSPIGATIVSDRVYEPFKHGTTSF
                     YHGYTFGGHPVSSAVALANLDIFEEEKLNERVRENSPLFRSALERLNDLPIVGNVRGD
                     GYFFGIELVKDKATKETFNDAESERLLRGFLSKALFDAGLYCRADDRGDPVIQLAPPL
                     ILEQPQFDEIEQILRSVLTEGMKIL"
     gene            complement(1556..2038)
                     /locus_tag="PLW15_05220"
     CDS             complement(1556..2038)
                     /locus_tag="PLW15_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT33835.1"
                     /translation="MSRPPRPPGSKPIALDDVSKAIIEQLQGDGRRSYAEIGKAVGLS
                     EAAVRQRVQKLTDSGVMQVVAVTDPMQLGFYRQAMIGIRVSGDTTTVAEKLGRLPAVD
                     YVVLTAGSFDILAEVVCENDEDLIELLNKQIRGIEGVQSTETFVYLKLLKQFYNWGTR
                     "
     gene            2163..3596
                     /locus_tag="PLW15_05225"
     CDS             2163..3596
                     /locus_tag="PLW15_05225"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HOT33836.1"
                     /translation="MAEIRNFINGEFVSARGDSSFEVIDPATEESYATSPVSGKADVD
                     AAYSAAAAAFEEWGETTPAERQLALFRIADAMEARADEFADLESKDTGKPRASLVADE
                     IMLSVDQIRFFAGEARHLSGVATGEYLKDHTSSIRREPIGVIGQVTPWNYPLNMAVWK
                     FAPAIAAGNTTVLKPSDTTPSSTLLLAEVAAEFLPPGVLNVITGDRTTGGLMIDHKTP
                     QMVSITGSVRAGMEVAKAAASDLKRVHLELGGKAPVIVFDDADIEAAVEGIGIAGYFN
                     AGQDCTAATRVLVHKNVHDEFVAALAAWAKDNAKTGAPDREDILFGPVNNANQLERVS
                     GIIDALPSNAKVELGGARQGDKGYFYEATIVSGLTQTDDAIQNEIFGPVITVQSFTDE
                     KQALEWANGVQYGLSSSVWTKDHARAMRFAKHLDFGCVWINTHIPLVAEMPHGGFKHS
                     GYGKDLSSYGFEDYTRIKHVMSFIGNS"
     gene            3663..4202
                     /locus_tag="PLW15_05230"
     CDS             3663..4202
                     /locus_tag="PLW15_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT33837.1"
                     /translation="MTDDLDDTVIVPPRDLPSVAPSAEPDVEHTRIVPKPAPTRIDHA
                     TGLPIVDEPQQTEEIAPLRYYAVRVSSRAEPIVLDAPCYVGRNPSAPRVVTGAPPRLI
                     RVPSPQREVSASHVEVRQLGATVVVTDLRSTNGSIVMVPGSVPRKLRQGESVVVSPGT
                     LVDIGDDNILQILPMQRPA"
     gene            4221..5054
                     /locus_tag="PLW15_05235"
     CDS             4221..5054
                     /locus_tag="PLW15_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT33838.1"
                     /translation="MTEIGASTARLAVPIPGHPGDAFVIAWAAATDTGRRRAANEDSY
                     VAQSPMFAVADGMGGHSAGDLASAAVVRRLAEAAVGDFAAPRAIERALEAATDDISVI
                     SGESELGVGTTVTGALLTLQDDEPYFAIFNVGDSRVYSFERNELTQVTVDHSVVQELV
                     ESGTLTRDEAEHHPDSNIITRAVGFNAAPIPDFWMIRAHAGLRLLLCSDGLTKEVSTE
                     RLRLHLAAGLSVLETAGALIDAALAAGGRDNITAIVIDVLDAPESVPDDDTAPRPARS
                     H"
     gene            5196..6626
                     /locus_tag="PLW15_05240"
     CDS             5196..6626
                     /locus_tag="PLW15_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT33839.1"
                     /translation="MARRLPSQPPTLPGFSHIHILGSGGFADVFLYEQNMPRRQVAVK
                     VMLSEVVNDQVRQMFQAEANLMAQLSAHPSILTVYQASVSSDGRPYLVMELCSSALSQ
                     RYRVERLPVAEVLRIAVKIGSAIETAHRAGVLHRDIKPSNILLTAYGHPVLSDFGIAA
                     TLSESDDREAVGMSIPWSAPEVLMDETAGTVASEVWAFAATVYSLLAGRSPFEVPGDS
                     NKSTDLISRINRAKPQPMDRNDVPQSLERALWRAMSRKPENRQSSILELVRELQAVET
                     EIGVPQTPVEVVMDDWALATVADLEDRTRIRGGVVPRGTVSQRRRRRRGGETTGDYSA
                     VGTLIKEDAPLPRSSGTRPQDRGGRFQTIAWILVAAAVLAVVLGTVATLVLLRSASSD
                     IPMVTAIETQITDNTVEFTWSDPGLGATDTYQVSTSDGGAPVQQRGTSFVVDAEPGDR
                     VCITVAVNREGKTGSPSSEKCADVPQ"
     gene            6637..>9345
                     /locus_tag="PLW15_05245"
     CDS             6637..>9345
                     /locus_tag="PLW15_05245"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT33840.1"
                     /translation="MIRKWISAHASLVISVTSGVVIAALVGAVAIVSTGYPAQQLELG
                     DGSVWVANSQQQAIGRANTEVLELNTVVASTGNDIQVLQQGSTVLLVDRTENKIDIVD
                     AATSEISDSVALPPNQPEVLLAGTRVAVFEQGTGELWLLNSADLSTFDSQSDSTLSLG
                     TDAMVSMDPAGMLYAYSPSAGLVYRVNAASSDGVDSSQSVTVPGTASSRSITSVGGHW
                     AILDTQTDVLLLDGRTVDLSAELGSGTGAVLQWPSIDGERVLIGYSGGLIAVSLGSGA
                     VSSLVTDTAGSPARPLSLAGCDYAAWSNGQNWRRCGGESEGVTAVLASMPGTARLSFE
                     TNGARAVLNDRRSGATWAVQRAGELIDNWDELITVDDQTPQEQQNDEEIPPEVEKAQQ
                     PPVALDDEFGARPGRSTVLPVLLNDYDPNGDVLVVSELTPIDEAVGRIDLITERQQIQ
                     LTLSPGASGQLRFDYSITDGRGGTATASVVVTIRGEAENSAPQQVRTTKAVVQAGGRV
                     TSQVLSDWVDPDGDAFYLTSASTADPDSVSYKPGGSVVFTDAGTGGDLKVVTIVVSDG
                     SAEATGTLAVTVKAAGEVPIVAEPFAVLAYAGQEVTLSPLDHVRGGSGTLRLNSVPAK
                     TDVTITPSYETGTFRFTSSAVRTHYLEFVVTDGTQTVTGLVRVDVAAPPDANTKPITV
                     PKTVFVKTLRNERIDVAGTDVDPAGGVLLVTGITNLPANSGVRAEVLEQRLVRVSLEA
                     PLDHPVTFNYRISNGLAEAEGVITVIEIPTPSRVQAPIATDDSVTVRVGQAIDIPVLR
                     NDEHPDGLDLTLQPTLEQQLPANSGLLFASGNVLRYLAPERTGNFTASYRVTGPDGQA
                     ATAVVRINVREEDQATNNAPVPATVTARVLAGETVRVKIPLTGIDPD"
BASE COUNT         1599 a         3063 c         3118 g         1565 t
ORIGIN      
        1 ccgggaagcg acgcacccgg gtcctctcgg aggacccggg tacgggcagt gcgttcgagg
       61 tcatgcgagc cttccggcgt cggcgtacgg gtcaggacgg gtccgagacc gatccaggga
      121 tcgacctaga ggatcttcat gccctcggtg agcaccgagc gcaggatctg ctcgatctcg
      181 tcgaactgcg gctgctcgag gatgagcggc ggcgcgagct ggatgacggg gtcgccgcgg
      241 tcgtcggcgc ggcagtagag gcccgcgtcg aacagcgcct tggacaggaa gccgcgcagc
      301 aggcgttcgg actcggcgtc gttgaaggtc tccttggtgg ccttgtcctt gacgagctcg
      361 attccgaaga agtagccgtc gccgcgcacg ttacccacga tcggcagatc attcaggcgc
      421 tcgagcgccg atcggaacag cggcgagttc tcgcgaacgc gctcgttgag cttctcctcc
      481 tcgaagatgt cgaggttggc cagcgcgaca gcggatgaca cggggtgccc gccgaacgtg
      541 tagccgtggt agaacgacgt cgtgccgtgc ttgaagggct cgtacacgcg gtcgctcacg
      601 atcgtggcgc cgatggggga gtatccgctg gtcattccct tggcgcaggt gatcatgtcg
      661 ggcacgtagc cgaactcgtc gcaggcgaac atgtggccga tgcgtccgaa ggcgcagatg
      721 acctcgtcgc tgacgagcag cacgtcgtac tggtcgcaga tctcgcgcac ccgctggaag
      781 tatccggggg gcggggggaa gcatccgccc gagttctgca ccggttcgag gaagacggcg
      841 gccacggtct cggggccttc gaagaggatc atctcctcga tgcggttggc ggcccacagc
      901 ccgaactcgc gttcggcggt ggccgagtcg gcaccggggg aggcgacgaa gcccatctcc
      961 tcggagcggt agaagttggt gttgggcacg cggaagccgc cgggcaccag gggctcgaac
     1021 atctccttca tgcccgggat gcccgtgatg gcaagcgctc cctgcggcgt gccgtggtag
     1081 gcgatcgacc gggagatcac cttgtgcttg gtcgggcgtc cctgcagctt ccagtagtac
     1141 ttggcgagct tgaacgcggt ctcgaccgcc tcgccgccgc cggtggagaa gaagactcgg
     1201 ttgaggtcgc cgggcgcgta ggaggccagg cggtcggcga gctcgatcgc gttggggtgc
     1261 gcgtacgacc agatcgggaa gaaggcgagt tcctcggcct ggcgggccgc gacatccgcg
     1321 agtcgcttgc gaccgtggcc ggcgttgaca acgaacaggc cgctgaggcc gtcgatgtac
     1381 ttgcgaccgg tggagtccca gatgtggtgg ccctcgccct tgacgatgat gggcactccc
     1441 gcaccgctct ccatgacgga ctggcgggag aagtgcatcc agaggtggtc cttggccttc
     1501 tgctgaaggt ccgcctcgtt gtaggtggtc aggctcggtt cggtcattgt catctctatc
     1561 tcgttcccca gttgtagaac tgcttgagca gcttcaggta gacgaaggtc tccgtggact
     1621 gcacgccctc gatgccgcgg atctgcttgt tgagcagctc gatgagatcc tcgtcgttct
     1681 cgcacacgac ctccgcgagg atgtcgaagc ttccggcggt cagcacgacg tagtcgaccg
     1741 cgggcaggcg tccgagcttc tcggcgacgg tggtggtatc tccggacacg cggatcccga
     1801 tcatcgcctg gcggtagaag cccagctgca tcggatcggt gacggccacg acttgcatga
     1861 cgccggagtc ggtgagcttc tgcacgcgtt gacgaaccgc ggcctcgctg agacccacgg
     1921 ccttgccgat ctccgcgtag gagcgtcgtc catcgccctg cagctgttcg atgatcgcct
     1981 tcgagacgtc atcgagcgcg atcggcttcg atcccggagg gcgcggagga cggctcatgg
     2041 ctcgattctg tcagtgtgcg agggtagtca caagcgattc cgcacctcag ccttgaaatg
     2101 actgctgaaa tcgttggtgt caacggtgga tgccacggcc gacgcccggc taagttagtg
     2161 ccatggcaga gatccgtaac ttcatcaacg gcgaattcgt ctccgcgcgc ggcgactcga
     2221 gtttcgaggt catcgacccg gcaacagagg agtcgtacgc gacatcccct gtgtcgggca
     2281 aggccgacgt cgacgcggcc tactccgcgg ccgccgcagc gttcgaggag tggggcgaga
     2341 ccactcccgc ggagcgccag ctcgcactgt tccgcatcgc cgacgcgatg gaggctcgcg
     2401 cggacgagtt cgccgacctg gagtcgaagg acaccggcaa gcctcgcgcc tccctcgtcg
     2461 ccgacgagat catgctctcg gtcgaccaga tccggttctt cgctggcgag gcgcgccacc
     2521 tcagcggcgt cgccaccggc gaatacctga aggaccacac ctcctcgatc cgccgtgagc
     2581 cgatcggcgt catcgggcag gtcaccccct ggaactatcc gctcaacatg gctgtgtgga
     2641 agttcgcccc ggcaatcgcc gcaggcaaca ccacggtgct caagcccagc gacacgacac
     2701 cgtcgtcgac gctcctgctg gcagaggttg cggccgagtt cctgccgccg ggagtgctca
     2761 acgtcatcac cggtgaccgc acgaccggtg gtctcatgat cgaccacaag acgccgcaga
     2821 tggtgtcgat cacgggctcg gtgcgggcgg gcatggaggt cgcgaaggcc gcggcatccg
     2881 atctgaagcg cgtgcacctc gagctcggcg gcaaggcacc ggtcatcgtc ttcgacgacg
     2941 ccgacatcga ggccgccgtc gagggtatcg gcatcgccgg ctacttcaac gccgggcagg
     3001 actgcaccgc ggcgacccgc gtgctcgtgc acaagaacgt gcacgacgag ttcgtggcgg
     3061 cgctcgccgc ctgggccaag gacaatgcca agaccggcgc tcccgaccgc gaggacatcc
     3121 tgttcggtcc ggtcaacaac gccaaccagc tggagcgtgt ctccggcatc atcgacgcgc
     3181 tgccgtccaa cgcgaaggtc gagctcggcg gggcgcgtca gggcgacaag ggctacttct
     3241 acgaggccac gatcgtgtcg ggcctcacgc agacggatga cgcgatccag aacgagatct
     3301 tcgggcccgt gatcaccgtg cagtccttca ccgacgagaa gcaggccctc gagtgggcca
     3361 acggggtgca gtacgggctg tcatccagcg tctggaccaa ggatcacgcc cgcgcgatgc
     3421 gcttcgcgaa gcacctcgac ttcggttgcg tgtggatcaa cacccacatc ccgctggtcg
     3481 ccgagatgcc gcacggaggc ttcaagcact cgggctacgg caaggacctc tccagctacg
     3541 gcttcgagga ctacacccgc atcaagcacg tcatgagttt cattggaaac tcatgaccag
     3601 cagacaccgt atgagtttca ttggaaactc atgaccagca gacaccgtat gagtttcatc
     3661 gggtgaccga cgacctcgac gacacggtga tcgtgcctcc gcgcgatctg ccgtccgtcg
     3721 cgccgtcggc cgagcccgac gtcgagcaca cccgcatcgt gccgaagcct gcgccgacgc
     3781 ggatcgacca cgccacgggg cttccgatcg tcgacgagcc tcaacagacc gaggagattg
     3841 cgccgctgcg ctactacgcg gtgcgggtgt cgtcgcgcgc cgaaccgatt gtgctcgacg
     3901 cgccgtgcta cgtcggtcgc aatccttcgg cgcccagagt tgtcaccgga gcgccgccgc
     3961 gtctgattcg agtgccgtcg ccccagcggg aggtctcggc atcccacgtc gaggtgcgtc
     4021 agctgggtgc cacggtggtg gtcaccgacc tcagatccac gaacggatcg atcgtcatgg
     4081 tgccgggcag tgtgccacgc aagctgcgtc agggtgagtc ggtggtagtg tctccgggca
     4141 cactggtgga tattggtgac gacaacatcc tccagatcct cccgatgcaa cgcccggcct
     4201 agcagggcgg agaggcagtc gtgaccgaga tcggcgccag cacggcgcgc ttggccgtgc
     4261 ccatccccgg gcaccctggc gacgcgttcg tgatcgcatg ggcggcggcc accgacaccg
     4321 gtcgcaggcg tgccgcaaac gaggacagtt acgtcgcgca gtcgcccatg ttcgccgtgg
     4381 cagacgggat gggcggccac tcggcggggg acctggccag tgcggccgtc gtgcggcggc
     4441 tcgccgaggc cgcggtgggg gacttcgctg cccctcgcgc catcgagcgc gcgctggagg
     4501 cggcaaccga cgacatttcg gtgatctcgg gggagagcga gctcggcgtc ggcaccactg
     4561 tcacgggtgc cctcctgacc cttcaagatg acgagccgta tttcgcgatc ttcaatgtcg
     4621 gcgacagccg ggtctactcg ttcgaacgca acgagctcac ccaggtgacg gtcgatcact
     4681 cggtggttca ggagctggtg gagtcgggca ctctcacgcg cgacgaggcc gagcatcatc
     4741 cggacagcaa catcatcacg cgggccgtcg gattcaatgc ggcacccatt ccggacttct
     4801 ggatgatccg ggctcacgcc gggctgcgtc tgctgctgtg ctccgacggg ctcaccaagg
     4861 aggtcagcac cgaacgcctc cgactgcacc tggcggcggg gttgtcggtg cttgagaccg
     4921 ctggcgcact catcgatgca gcgctcgcag ccgggggccg cgacaacatc acggcgatcg
     4981 tcatcgacgt tctggatgct ccggagtcgg ttcccgacga cgacacggca ccgcgtccag
     5041 cccgctcgca ctgagttcac aggtaccgac cgcggcccct cgacggccca gaagtggggg
     5101 tcggtcgtgc gacccctttt aggggtacac ggcgaccatg cctgcggggt acctgcctac
     5161 aatggtcgga tacgtccaga ggcgaggggg tgttcgtggc gcgccgactg ccatcccaac
     5221 ccccgacgct gccgggattc tcccacattc acattctggg ctccggcgga ttcgcggacg
     5281 tcttcctgta cgagcagaac atgcctcgtc gtcaggtcgc cgtcaaggtc atgctcagcg
     5341 aggtcgtcaa cgaccaggtg cggcagatgt tccaggccga ggcgaacctg atggcccagt
     5401 tgagcgcgca tccctcgatc ctcacggtgt atcaggcgag cgtgtcatcc gacggcaggc
     5461 cctacctggt gatggaactg tgttcctcgg ctctgagtca gcgctaccgc gtcgaacggc
     5521 tgcccgtcgc cgaggtgctg cgcatcgccg tgaagatcgg tagcgcgatc gagaccgcgc
     5581 accgtgcggg ggtgctccat cgcgatatca agccgtccaa catcttgctt accgcctacg
     5641 gacaccccgt gttgtccgat ttcggcatcg ccgccaccct gagcgagtcc gacgaccgcg
     5701 aggccgtggg catgtcgatt ccgtggtcgg cgcccgaggt gctcatggac gagacggcgg
     5761 gcaccgtggc atccgaggtg tgggccttcg cggcgacggt ctactcgctg ctcgcggggc
     5821 gctcgccgtt cgaggtgccc ggcgactcca acaagtcgac cgatctcatc tcgcgcatca
     5881 accgtgcgaa gccgcagccg atggatcgca atgatgtgcc ccagagcctg gagcgcgccc
     5941 tctggcgggc catgtcccgc aagcccgaga accgccagtc cagcatcctg gagctggtga
     6001 gggaattgca ggcggtggag accgagatcg gtgtgccgca gacacccgtg gaggtcgtca
     6061 tggacgactg ggcactcgcg acggtcgccg accttgagga ccgcaccagg atccgtgggg
     6121 gcgtcgtccc acgggggacg gtctcacagc gccgtcgtcg gcgacgcggt ggggagacca
     6181 ccggtgacta cagcgcggtg ggaacactca tcaaggagga cgccccgttg ccccggagtt
     6241 cgggcacgcg gccccaggat cgcggtggcc gattccagac gatcgcgtgg atcttggttg
     6301 cggcggcggt actcgcggtc gtcctcggta cggttgcgac actggtgctg ttgcgatcgg
     6361 catcgtcgga catcccgatg gtgaccgcaa tcgagacgca gatcaccgac aacaccgtcg
     6421 agttcacgtg gagtgacccg ggccttggtg ccactgacac gtatcaggtg agcacgtcgg
     6481 acggaggcgc acccgtgcag cagcgcggca cgagcttcgt ggtcgacgcg gagcccggcg
     6541 accgggtctg catcacggtc gcagtgaacc gtgaaggtaa gaccggatcg cccagcagcg
     6601 agaagtgcgc tgacgtaccc caatgacggt gatcacgtga tcaggaagtg gatttccgcc
     6661 cacgcgtcgc tcgtcatcag cgtgaccagc ggtgtcgtca tcgccgccct ggtcggggcg
     6721 gttgccatcg tgtcgacggg ctaccccgcg cagcaactcg aactgggtga cggctcggtc
     6781 tgggtcgcca acagccagca gcaggcgatc ggtcgcgcca acaccgaggt gctcgagctc
     6841 aacaccgttg tcgcgtcgac aggcaacgac atccaggtgc tccagcaggg ctccacggtg
     6901 ctgctggtcg atcgcaccga gaacaagatc gacatcgtgg atgccgcgac ctctgagatc
     6961 agcgacagcg tcgcgctccc acccaaccag cccgaggtgc tcctggctgg cacgcgtgtt
     7021 gccgtcttcg agcagggcac cggcgaactc tggctgctga actcagccga cctgtccacc
     7081 ttcgactccc agtccgattc gacgttgagc ctgggcaccg acgccatggt gagcatggat
     7141 ccggcgggaa tgctctacgc ctattcgccg tcggccgggc tcgtctatcg agtgaacgcc
     7201 gcatcctccg acggtgttga cagttcgcag tcggtgacag ttcccggcac ggcgtccagc
     7261 cgcagtatca cctccgtcgg cggacactgg gcgattctcg acacccagac cgacgtgctc
     7321 cttctcgacg gtcgcaccgt cgacctctcg gccgagctgg gatccggcac gggtgcggtg
     7381 ctgcagtggc cgagcatcga cggtgagcgc gtgctcattg gctactcggg gggactcatc
     7441 gctgtctcgc tgggcagtgg tgctgtgtca tccctggtca cggacaccgc aggtagcccg
     7501 gctcgcccgc tgagccttgc aggatgcgac tatgccgcgt ggtcgaacgg gcagaactgg
     7561 cgcaggtgcg gtggtgaatc cgagggcgtg actgcggtgc tcgcctcgat gccgggtacc
     7621 gcccgactga gcttcgaaac gaatggtgcc cgggccgttc tcaacgatcg gcggtcgggt
     7681 gccacctggg cggtgcaacg cgccggggag ctcatcgaca actgggacga gctcatcacg
     7741 gtcgacgacc agacgcccca ggagcagcag aacgacgagg agatccctcc cgaggtggag
     7801 aaggctcagc agcctcccgt cgctctcgac gacgagttcg gcgcgcgtcc cggcaggtcg
     7861 acggtgcttc cggtactgct caatgactat gacccgaacg gcgacgttct ggtggtgtcc
     7921 gagctcacgc ccatcgatga ggctgtcggt cgcatcgacc tcatcaccga acggcagcag
     7981 atccaactca ccctctctcc cggcgcctcg ggacagctgc ggttcgacta ctccatcacc
     8041 gatgggcggg gcggaactgc aacggcgagc gtcgtcgtca ccattcgagg cgaagccgag
     8101 aactccgcgc cgcagcaggt gcgcacgacc aaggccgtcg tgcaggctgg cggtcgcgtc
     8161 acctcccagg tgctctcgga ttgggtcgat cccgacggcg acgcgttcta cctcacgagc
     8221 gccagtacgg ccgacccaga ctccgtcagc tacaagccgg gcggctcggt cgtcttcacc
     8281 gatgcgggca ccggcggcga cctcaaggtt gtgaccattg tcgtttcaga cggcagcgcc
     8341 gaggcaaccg gcacgctcgc cgtcaccgtg aaggccgcgg gcgaggtgcc catcgtggcc
     8401 gagcccttcg cggtgctcgc ctacgccgga caggaagtca ccttgagccc actcgaccac
     8461 gttcgtggtg gatcgggcac actgcggctg aacagcgtgc ccgccaagac ggatgtcacg
     8521 atcaccccga gctatgagac cggaaccttc cgcttcacga gctcggcggt gcgcacccac
     8581 tacctggaat tcgtcgtcac cgatggaacg cagaccgtca cagggctcgt gcgcgtggat
     8641 gtcgcggccc cgcccgacgc gaacacgaag cccatcacgg tgcccaagac cgtattcgtc
     8701 aagacactgc gcaacgaacg catcgatgtc gccggcactg acgtcgatcc ggcgggcggc
     8761 gtgctgctgg tcacgggaat caccaatctg cccgcgaact cgggagttcg agctgaggtg
     8821 ctcgaacagc gtctcgtgcg cgtgagcctc gaggcgccgc tggatcaccc ggtgaccttc
     8881 aactaccgaa tcagcaacgg cctcgccgag gccgagggcg tgatcacagt catcgagatc
     8941 ccgactccga gccgcgttca ggcgccgatc gccactgacg acagcgtgac ggtgcgcgtg
     9001 gggcaggcga tcgacatccc cgttctgcgc aacgacgagc atccggacgg cctggatctg
     9061 accctgcaac ccaccctcga acagcagctg cccgcgaact cgggtctgct cttcgcgtcg
     9121 ggcaacgtgc tgcggtacct tgctcccgaa cgcaccggca acttcacggc gagctaccgg
     9181 gtcaccggac ccgacggtca ggcggcgacg gccgtggtga ggatcaacgt tcgcgaggag
     9241 gaccaggcca ccaacaacgc gccggtgcct gcgacggtca ccgctcgggt tctcgcgggg
     9301 gagacggttc gggtgaagat tccgctgacg gggatcgatc cggat
//
LOCUS       DAOSOU010000067         9318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7344_length_9318_cov_5.258987, whole genome shotgun sequence.
ACCESSION   DAOSOU010000067 DAOSOU010000000
VERSION     DAOSOU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7344_length_9318_cov_5.258987"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLW15_05250"
     CDS             <1..325
                     /locus_tag="PLW15_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33841.1"
                     /translation="LIIGILAAIAIPVFLGQQDQAKNAAIQSDLGNAKIAMISYATAN
                     GGTYLGVSNQAATATALGTYGYTGSSVAVITGGASFCLQGTSAATGSPAYKITYNGGA
                     VAGTC"
     gene            434..868
                     /locus_tag="PLW15_05255"
     CDS             434..868
                     /locus_tag="PLW15_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT33842.1"
                     /translation="MFTPQAKSASANDEGFTLVEVVVSIIVLALIALALLPLLIQGIK
                     QSAQAAAIASAVQLANSQVDLARTQATTCTAVTATPTVAVSSSATYRGVPLVVTKTVG
                     ACPTPTPSATAPGTIPFTATVTRSDTGETLATVSTKILVTGG"
     gene            870..1460
                     /locus_tag="PLW15_05260"
     CDS             870..1460
                     /locus_tag="PLW15_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT33843.1"
                     /translation="MIPQSNDRQSGFTLVELMVGALLTVLVLTIVGGLVISSTRSEGI
                     VRSVAQATQAGQVVTASVQNGIRNSAISLNTGTPYRLTVPSGSDQLLVALVVSSGTSA
                     TASCSAWYYSASAGEVRYTSSSSAIAAPTAGQLATWTLLSKGVTPVSPSTTIFTANGT
                     YGLTLAFQEAAGSASPIVFQSSITSRTGMTGAIACY"
     gene            1451..3235
                     /locus_tag="PLW15_05265"
     CDS             1451..3235
                     /locus_tag="PLW15_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33844.1"
                     /translation="MLLTRIRSALTRDDRGAALAAAMSLMLLGALLSVLILSSVVATF
                     SATTGTRATVQSEASAQAGIDVALASVMSGNCVSSYTSTTVPKYSVAVSYTTSPTLST
                     PGLTWVSGCPTASAPFMKITSTGTAGSPAVGTTTFGNSEAVEAIYARPVSTAILQATG
                     PAVYAYSSQSFAGSGTLVSLDGTNDADVMIKTGDVACSGGAAAKGDFVVNEGNLTLSG
                     SCGVTGNVWASGSGKGVINLSGGVVVGGNAVGNGLIISSGSVGGSTWTTGATTINSAT
                     IGGGVTVTANNFVTGGSNKIGGNLWASGSAELTNGDDVKGNATAQTLKLSGGNVGSSA
                     SNEAWSRGDVTGVTWFTIKSHLTAKSVPSGMTAGGGITVVPAGPGAGGAAPATPTSPI
                     VPNWVNFTYQTSDWPGYTVATLGTTCTSTTLQTAINSFSGGKGVIDARACTAPIFMGG
                     STKVALSNDVAIISTYGFDLSYSKGGGGFSSTVPVDLFLITPDTTAESPATPTCPSGS
                     SILVGGGFTFSSTISTIIYTPCKAAIGSGINFYGQVFAGQAVVDGAAKLFYTTVGLPG
                     WNLDTGTTSSVTSSPNAWASVSTRNVTG"
     gene            3235..4059
                     /locus_tag="PLW15_05270"
     CDS             3235..4059
                     /locus_tag="PLW15_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOT33845.1"
                     /translation="MVVAVIGIFGALIGSFLNVVIYRVPLGRSVVAPASACGQCGHPI
                     RWYDNIPVISWLVLRGRCRDCGAHISVRYPLVELAGAVAFVFVALRFVPEISAATSVA
                     AMVAGILALVGYLYFVAISLALALIDLDTQRLPNVLVLPSYAVAAVTLGVSSIIGGSL
                     PALLPAAVGGVGLFLVYLALASAWRGGMGFGDVKLAGVIGIYLGWLGWDVLAVGALGA
                     FLLGGVFGVILLVSRSAGGKTAVPFGPWMLGGAWVGILGGHEIAAWYLGLFGLGVS"
     gene            4056..5099
                     /gene="pilM"
                     /locus_tag="PLW15_05275"
     CDS             4056..5099
                     /gene="pilM"
                     /locus_tag="PLW15_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOT33846.1"
                     /translation="MTARIVGLDIGSASVRAVEVKNPDKPSRMILKTQELPLPEGSVS
                     RGEVIDVSTVSMTIRRLWAMGGFGSKDVVLGMGGPRVLSRELTMPRAPIGQLRESLQF
                     HVQDLLPVPVAEAILDFYPVSEANGDNGPVINGLLVAAVKEAVNANIAAVTRAGLRVA
                     QVDLIPFALARALAPVGVARGLEAIVSMGANSTNVVITESGVPHFVRIIPSGGDDITR
                     ALSTRLKLALQAAELTKRAVGLDAANATPDQRPAVEIVYEIAGEQLTSIRNTLTFHLN
                     SHPGAQLTRIILAGGGSELRGVGRALAEMTGVQVVQADPLGGVPVGKQMRASREQVVA
                     LTTAIGLTLGVEE"
     gene            5096..>5417
                     /locus_tag="PLW15_05280"
     CDS             5096..>5417
                     /locus_tag="PLW15_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33847.1"
                     /translation="MSKRTERDAVVIGGEPRVNLLPPEVALRKKARSARRGLVALVII
                     VLLAVAGGYGFATVRALAVEAELAVSQARTAELLAEQLQYADVTAVSSNVQAVKDART
                     VAVST"
     assembly_gap    5418..5427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5428..5769
                     /locus_tag="PLW15_05285"
     CDS             <5428..5769
                     /locus_tag="PLW15_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33848.1"
                     /translation="LWNEYYTKIRDLIPVDTAISSFVVDGRAPWEPDNVPAGPLRQPR
                     VATITFVIASAGPFPVQPFVAQISKIPGFVDVTADVITKDGESYTTQFTYNLGADALS
                     GRFPAGEEASN"
     gene            5769..6416
                     /locus_tag="PLW15_05290"
     CDS             5769..6416
                     /locus_tag="PLW15_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33849.1"
                     /translation="MSNRLWIFGAVLASIAIVVLGWVVGISPKLAEGDAAATQRQNVD
                     SQNLAQEQANVLLKEQFSKIKTYQDELKKLQVSVPASAALDVFSDQIVTDAAANGLIL
                     TNLTSLEAAVYGGSVDPTAATNPDTVKPEESTATDPNAPAAGLNPSLTNRFFTIGVSL
                     KVTGGADQIFTFAKGLQGDKRLFLVTDIQFTTDGEPGATITGFIFVVADPPAAAQ"
     gene            6490..7233
                     /locus_tag="PLW15_05295"
     CDS             6490..7233
                     /locus_tag="PLW15_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33850.1"
                     /translation="MSDAPVSPEPKKPRTTKPEVIEDAVVIEEPATTAASSEPTKVEP
                     VVIDSPPVETPSPQVVYVQTPAPPKKKGNRGIGSVLAIASGLIFAAAFALVVAAIANL
                     SGIGRFTVTFLAQPSFYVPVLFYIIGAVLLVIIVNRAGWAAYVLGSIFVGLVVYFGTV
                     GVLLLGQGVILKTPSEAAALLALGLTNPLVIAAGLVAREVSMWTGALISRRGRRLKVR
                     NATAHETWEREVAQKKAEHERTSAAATAV"
     gene            7199..7624
                     /locus_tag="PLW15_05300"
     CDS             7199..7624
                     /locus_tag="PLW15_05300"
                     /inference="COORDINATES: protein motif:HMM:NF040330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6121 family protein"
                     /protein_id="HOT33851.1"
                     /translation="MSAPPQLPPPSNPALLPVLAAVVYVAAVVAGWGILSLLLDRDVI
                     DYPDAGPLLGPAMAVAGGVVTWLVLWRRPARVLAAVAIAGFGTYLAVILIGAIGYTVT
                     RADGTWMLLAGAHFALSPFVAAAAGLSALTVLVVRAVQP"
     gene            7877..8251
                     /gene="rpsL"
                     /locus_tag="PLW15_05305"
     CDS             7877..8251
                     /gene="rpsL"
                     /locus_tag="PLW15_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011096633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT33852.1"
                     /translation="MPTIQQLVRKGRTPKVVKTKAPALRSNPQQRGVCTRVYTTTPRK
                     PNSALRKVARVKLSNGTEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGAL
                     DTQAVKNRKQARSRYGAKMDKK"
     gene            8251..8721
                     /gene="rpsG"
                     /locus_tag="PLW15_05310"
     CDS             8251..8721
                     /gene="rpsG"
                     /locus_tag="PLW15_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOT33853.1"
                     /translation="MPRKGPAPKRPVVADPVYGAPIVSQLVNKILLDGKKGLAERIVY
                     GALEGVSAKNGQDAVVTLKKALDNVRPTIEVKSRRVGGSTYQVPVEVKAHRANTLALR
                     WLTSYAKGRREKTMTERLTNEILDASNGLGAAVKRREDTHKMAESNKAFAHYRW"
     gene            8832..>9318
                     /locus_tag="PLW15_05315"
     CDS             8832..>9318
                     /locus_tag="PLW15_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT33854.1"
                     /translation="MAQDVLTDLNKVRNIGIMAHIDAGKTTTTERILFYTGITHKIGE
                     VHDGAATMDWMAQEQERGITITSAATTCFWDKHQINIIDTPGHVDFTVEVERSLRVLD
                     GAVAVFDGKEGVEPQSETVWRQADKYDVPRICFVNKMDKLGADFYFTVDTIIKRLGAK
                     PL"
BASE COUNT         1596 a         3219 c         2884 g         1609 t
ORIGIN      
        1 cctgatcatc ggcatcctcg ccgcgatcgc catcccggtg ttcctcggcc agcaggacca
       61 ggccaagaac gccgcgatcc agtccgacct cggcaacgcg aagatcgcca tgatctccta
      121 cgcgactgcc aacggtggca cctacctcgg tgtcagcaac caggccgcga ccgcgaccgc
      181 actcggcacc tacggctaca ccggcagctc ggtcgccgtc atcaccggtg gcgccagctt
      241 ctgcctccag ggcacctcgg ccgcgaccgg cagcccggca tacaagatca cctacaacgg
      301 cggcgccgta gcaggtacct gctaagcacc acctagcacg acctgtgggg cgctgctccg
      361 gcggcgcccc acagtcatca ccacagcact gaagcatcac cagcacgaca cgatcagagg
      421 ggggctcgat cgcatgttta caccgcaggc gaagtcggcc tccgcgaacg acgagggctt
      481 cacactcgtc gaggtcgtcg tctctatcat cgttctcgca ctcatcgcgc tcgcactgct
      541 gccgctgctc atccagggga tcaagcagtc agcacaggcc gcggccatcg ccagtgccgt
      601 gcagctcgcc aacagtcagg tagacctggc ccgtacgcag gcgaccactt gcaccgctgt
      661 caccgccacg ccgacggtcg cggtgtcgtc ctctgccacg taccgaggtg tgccgctcgt
      721 cgtcaccaag accgtgggcg cctgccccac tccgacgccg agcgctaccg caccgggcac
      781 gatcccgttc accgccacgg tgacccgaag cgacaccggc gagactctcg cgacggtgtc
      841 caccaagatc ctcgtcacgg gcggctgacg tgatcccgca gtcgaacgat cggcaatccg
      901 gcttcaccct cgtcgagctc atggtgggtg ctctgctcac ggtgctcgtg ctcacgatcg
      961 tgggcggact tgtgatcagc tcgacccgtt cggagggaat cgtcaggtca gtggcgcaag
     1021 cgactcaggc gggtcaggtc gtcacagcct ccgtgcagaa cggcatccgc aactccgcga
     1081 tctcgctgaa caccgggacg ccgtaccgac tcaccgtccc gagcgggtcg gaccagctcc
     1141 tcgtggcgct cgtcgtctcc tcgggtactt cggcgaccgc gagctgttcg gcctggtact
     1201 actcggcgag cgcgggtgag gtgcgctaca cgtcgtcgtc gtcggccatc gctgcgccga
     1261 ccgcaggtca gctcgctacc tggacgctgc tttccaaggg cgtcacgccg gtatcgccga
     1321 gcaccacgat cttcacggcg aacggcacat atggactcac tctcgcgttc caggaggctg
     1381 ccggttcggc gtcgccgatc gtgttccaga gttcgatcac cagccgcact ggaatgacag
     1441 gagccatcgc atgctactga cccgaattcg ctccgccctc acgcgcgacg atcgaggtgc
     1501 cgcactcgcg gcggcgatgt cgctcatgct tctgggcgcg ctcctgtcgg tgctcatcct
     1561 ctccagcgtg gtggccacct tctccgcgac cacgggaacc cgcgcgaccg tgcagtccga
     1621 ggcatccgca caggccggaa tcgatgtcgc gcttgcctcc gtcatgtcag gcaactgcgt
     1681 gtcgagctac accagcacca ctgttcccaa gtactccgtg gccgtgagtt acaccacctc
     1741 accgactctc agcacgcccg ggttgacctg ggtcagcgga tgcccgaccg cgtcggcgcc
     1801 cttcatgaag atcacgtcga cgggaaccgc gggcagtccc gccgtaggca ccacgacatt
     1861 cggcaactcc gaggccgtcg aggcgatcta cgcccggccc gtcagtacgg cgatccttca
     1921 ggccactggg ccggccgtct acgcgtattc ctcacagtcg ttcgctggtt caggcactct
     1981 ggtgtcgctc gatggcacga acgacgctga tgtgatgatc aagaccggcg atgtcgcttg
     2041 ctcgggcggt gcggcagcca agggtgactt cgtcgtcaac gagggcaatc tcacgctcag
     2101 cggttcgtgc ggagtcaccg gcaacgtctg ggcctcgggt agcggaaagg gagtcatcaa
     2161 cctgtccggt ggtgtggtcg tcggcgggaa tgcggtggga aacggactca tcatcagttc
     2221 tggatctgtc ggtggttcga cctggaccac aggtgccaca accatcaaca gcgcgacaat
     2281 cggcgggggc gtcaccgtca cggcgaacaa ttttgtcacg ggcggcagca acaagatcgg
     2341 cggcaacctg tgggcgtccg gaagcgcaga gttgaccaac ggcgacgacg tcaagggcaa
     2401 tgcaaccgct cagacgctca agctcagcgg cggcaacgtc gggtcgtctg ccagcaacga
     2461 agcatggtcg cgcggcgatg tgacgggagt tacctggttc acaatcaagt cgcacctcac
     2521 ggcgaaatca gttccctcgg gcatgacagc ggggggcggg atcacagtcg tacccgcagg
     2581 cccgggcgct ggcggtgccg caccagcaac gcccacctcg ccgatcgtgc cgaactgggt
     2641 caacttcacc taccagacat cggattggcc ggggtatacg gtcgcgaccc tcggcacaac
     2701 ctgcacgtca acgacccttc agaccgcgat caacagcttc agtggcggca agggtgtcat
     2761 cgatgctcgg gcatgcaccg ccccgatctt catgggcggc agcactaaag tcgcgctgtc
     2821 gaacgacgtc gccatcatct cgacctacgg cttcgatctc agctacagca agggtggcgg
     2881 tggtttcagc tcgaccgtgc cagtcgacct cttcctcatc acgcccgaca ccacggcgga
     2941 gtcgccagca acaccgacgt gcccgagtgg atcgagcatc ctggtgggcg gtgggttcac
     3001 cttcagcagc accatctcga cgatcatcta cacgccgtgc aaggcggcga tcggatccgg
     3061 catcaacttc tacggccagg tcttcgcggg tcaggcggtc gtggacggcg ctgcgaaact
     3121 gttctacacg acggtcgggc tgcccggctg gaacctcgac accggaacca cctccagtgt
     3181 cacctcgtcg cccaatgcgt gggcgtccgt ttcgacccgg aatgtgacgg ggtgatcgtg
     3241 gtcgccgtca tcggaatctt cggtgccctc atcgggtcct tcctgaacgt cgtgatctac
     3301 cgcgtgccgc tcggccgttc ggtcgtggcg cccgccagcg cctgcggcca gtgcgggcat
     3361 cccatccgct ggtacgacaa catccccgtg atctcctggt tggtgctccg cggtcgttgc
     3421 cgcgactgtg gggcgcacat ctccgtgcgc tacccgctcg tcgagctcgc tggcgccgtc
     3481 gccttcgtct tcgtcgccct gcgcttcgtg ccggagatct cggccgcgac ctcggtggcg
     3541 gcgatggtcg ccggcatcct cgcgctcgtc ggctacctgt acttcgttgc catcagcctc
     3601 gcgctggcac tcatcgatct cgacactcag cggctgccca acgttctcgt gctgccgtcg
     3661 tacgctgtcg ccgccgtgac tctgggcgtt tcttcgatca ttggcggatc gttgcccgct
     3721 ctcctgccgg ctgctgtcgg cggtgttgga ctgttcctcg tctacctcgc tctggcgtcg
     3781 gcctggcgcg gcggtatggg gttcggtgac gtcaagctcg cgggcgtcat cggcatctat
     3841 ctgggatggc tcggctggga cgtgctcgcc gtgggcgcgc tcggggcgtt ccttcttggc
     3901 ggcgtcttcg gcgtgatcct cctcgtgtcg cgctcggccg ggggcaagac ggctgtaccg
     3961 ttcggaccgt ggatgctcgg gggagcctgg gtgggcatcc tcgggggcca tgagatcgcc
     4021 gcctggtatc tcggactgtt cggattggga gtgtcatgac cgcgcgtatc gtcggcctcg
     4081 acatcggaag cgccagcgtg cgcgcggtgg aggtgaagaa ccccgacaag cccagccgta
     4141 tgatcctgaa gacgcaggaa cttccgctgc ccgaggggtc ggtcagccgc ggcgaggtca
     4201 tcgacgtgtc gacggtctcc atgaccatcc gtcgcctctg ggcgatgggc ggcttcggct
     4261 cgaaggatgt tgtgctcggc atgggcggcc cccgagtgct ctcccgcgaa ctcaccatgc
     4321 cccgcgcgcc gatcggccag ctccgggagt ccctgcagtt tcacgtgcag gacctgcttc
     4381 cggtgcccgt ggccgaggcg attctcgact tctaccccgt gagcgaggcc aacggcgaca
     4441 atggcccggt catcaacggc ctccttgtcg ccgccgtcaa ggaggcagtc aacgcaaaca
     4501 tcgcggcggt cacccgcgcc ggcctgcgag ttgcgcaggt cgacctcatc cccttcgcgc
     4561 tggcgcgcgc gctcgcgccg gtaggcgtcg cccgcggcct tgaggcgatc gtgagtatgg
     4621 gcgcgaactc cacgaatgtc gtgatcaccg agagcggagt accgcacttc gtgcgcatca
     4681 tcccgtcggg cggcgacgac atcacccgcg ccctgtcaac gagactcaag cttgcgctgc
     4741 aggccgcgga actgaccaag cgcgcggtcg gcctcgatgc cgccaacgcg acccctgacc
     4801 agcggcccgc cgtcgagatc gtctatgaga tcgcgggcga gcagctcacc agcatccgca
     4861 acacgctcac ctttcacctg aactcgcacc ccggagcaca gctgacccgc atcattctcg
     4921 cgggcggagg ctcagaactc cgcggagtgg gccgtgctct ggccgagatg acgggcgtgc
     4981 aggttgtcca ggccgatcca ctcggtggtg ttccggtggg caagcagatg cgcgcgagtc
     5041 gcgagcaagt cgtggccctc accacagcga tcggcctcac cttgggggtg gaggaatgag
     5101 caagcgcacc gagcgggacg ccgtcgtaat cggtggcgag ccgcgcgtca atctcctccc
     5161 gccggaggtc gccctgcgca agaaggcgcg atccgctcga cgcggcctcg tcgccctcgt
     5221 gatcatcgtg ctgctcgccg tcgccggcgg ctacgggttc gcaaccgttc gcgctctagc
     5281 cgtggaggcc gagctcgccg tgtcccaggc gcgcaccgcc gagcttctgg ccgagcagct
     5341 ccagtacgcg gacgtcaccg ctgtgagcag caacgtgcag gccgtcaagg atgcccgcac
     5401 cgtcgcggtc tccaccgnnn nnnnnnnctc tggaacgagt actacacgaa gatccgcgac
     5461 ttgattccgg tggacactgc catcagcagt ttcgttgtcg atggccgcgc tccctgggag
     5521 cctgacaacg tacccgccgg accgttgcgc cagccgaggg tcgccaccat caccttcgtg
     5581 atcgcgtccg ctggcccgtt cccggtgcaa ccgttcgtcg cgcagatctc gaagatccct
     5641 ggttttgtgg atgtcaccgc tgacgtgatc accaaggatg gcgagtccta cacgacgcag
     5701 ttcacctaca acctgggagc cgacgcactc tcgggccgtt tccccgccgg tgaggaggcg
     5761 agcaactgat gtcgaaccga ctgtggattt tcggggcggt gctcgccagt atcgcgatag
     5821 tcgtgctcgg ctgggtcgtc ggcatctcac ccaagctcgc cgagggcgac gcggctgcga
     5881 cccagcgcca gaacgtggac agccagaact tggcgcagga gcaggcgaac gtgctcctca
     5941 aggagcagtt ctccaagatc aagacctacc aggacgagct caagaagctt caggtctcgg
     6001 tgcccgcgtc ggcagcactg gatgtgttct cggaccagat cgtcacggac gcggcggcca
     6061 atgggctgat actgacgaac ctcacgtcac ttgaggcggc cgtttacggc ggttccgtgg
     6121 atccaaccgc ggcgaccaat cccgacacgg tgaagccgga ggagtccacc gctaccgacc
     6181 ccaacgcgcc ggcggccggc cttaacccgt ctctgacgaa ccgattcttc acgatcggcg
     6241 tgtccctgaa ggtcaccggt ggcgccgacc agatcttcac cttcgcgaag ggcctgcagg
     6301 gtgacaagcg gctgtttctg gtcaccgaca tccaattcac taccgatggc gaaccgggtg
     6361 caaccatcac cgggttcatc ttcgtggtcg ccgacccccc tgccgccgct caatagcaac
     6421 ccctgtctcc cgtgcagccg cgctcactag actgagcgcg catatcccac gaggtaggag
     6481 gagtaggcaa tgagcgacgc accagtgtca cccgagccca agaagccccg caccaccaag
     6541 ccggaggtca tcgaggacgc tgtggtcatc gaggagcccg caaccacggc tgcttcgagc
     6601 gagcccacca aggtcgagcc cgtcgtcatc gacagcccgc ctgtcgagac cccctcgccc
     6661 caggtcgtct atgtgcagac tcccgccccg cccaagaaga agggcaaccg cggtatcggt
     6721 tcggttctcg ccatcgcatc cggcctcatc ttcgcggcgg ccttcgcgct ggtggtcgcg
     6781 gcaatcgcca acctcagcgg catcgggcgc ttcaccgtca ccttcctggc gcagcccagc
     6841 ttctacgttc ccgtgctgtt ctacatcatc ggcgcggtac tcctggtgat catcgtgaac
     6901 cgcgccggat gggccgccta cgtgctcggc agcatcttcg tgggtctggt ggtctacttc
     6961 ggcaccgtgg gcgtgctgct actcggccag ggcgtcatcc tgaagacccc gtccgaggcc
     7021 gcggcgctgc tcgctctggg gctcaccaac ccgctcgtca tcgcggccgg cctcgtggcg
     7081 cgtgaggtct cgatgtggac gggcgctctg atcagccgtc gcggacgccg cctcaaggtg
     7141 cgcaacgcca ccgcccacga gacgtgggag cgcgaagtcg cccagaagaa ggcggagcat
     7201 gagcgcacct ccgcagctgc caccgccgtc taatccggcc ttactgcccg tactcgccgc
     7261 tgtcgtctac gtcgcggccg tcgtagcagg ctgggggatt ctcagcctgc tgctcgaccg
     7321 cgacgtgatc gactacccgg atgcgggccc actgctcggc ccggccatgg ccgttgccgg
     7381 cggtgtcgtg acgtggctgg tgctctggcg acgcccggcg agggtgctcg cggcggtcgc
     7441 gatcgccgga ttcggaacct atctcgcggt catcctcatc ggcgcgatcg ggtacacggt
     7501 gacccgcgca gacggcacat ggatgctgct ggccggcgca cacttcgcgc tcagcccgtt
     7561 cgtggccgct gccgctgggc tctcggcact gaccgtgctg gtcgttcgag cggttcagcc
     7621 gtgagcgcaa tcatttgacc ggcgtgtccg gcaagactat tcttggcgag gtgtgcgctt
     7681 tgtcgtgcgc ttgaagcatg ccttcgagtc attgcacttc gcaccaccgg gttcgctcat
     7741 cgaacgaccc ccacggcata cccacaaatc tcggcgaaag ctcgacctgt gggtccagat
     7801 gaactcgacc ggcccgccag tcgaatgcta accatcaaga cgctgtcacc gtgcagcaat
     7861 tacaaggagt tacttagtgc ccaccattca gcagttggtg cgtaagggac gcacgccgaa
     7921 ggtcgtcaag accaaggcgc ccgccctccg ctccaacccg cagcagcgcg gcgtttgcac
     7981 gcgtgtctac acgacgaccc ccaggaagcc gaactcggct ctgcgcaagg tcgctcgcgt
     8041 caagctctcc aacggaaccg aggtcacggc ctacattccc ggcgagggtc acaaccttca
     8101 ggagcactcg atggtcctgg tccgcggcgg tcgcgtgaag gacctccccg gcgtgcgcta
     8161 caagatcatc cgtggcgccc tcgacaccca ggcggtcaag aaccgtaagc aagcgcgcag
     8221 ccgctacgga gcgaagatgg acaagaagta atgcctcgta aaggaccagc accgaagcgt
     8281 cccgttgtcg cggacccggt ctacggagct cccatcgtga gccagctcgt caacaagatc
     8341 ctgctcgatg gcaagaaggg cctcgccgag cgcatcgtct acggtgcact cgagggcgtc
     8401 tctgccaaga acggccagga tgctgtcgtc accctcaaga aggcgctcga caacgtgcgc
     8461 cccaccattg aggtcaagtc ccgccgcgtc ggtggctcga cgtaccaggt tccggtcgag
     8521 gtcaaggcgc accgcgccaa caccctcgcc ctgcgctggc tcacgagcta cgccaagggt
     8581 cgtcgcgaga agacgatgac cgagcgtctc accaacgaga tcctcgacgc ctcgaacggc
     8641 ctcggtgccg cggtcaagcg ccgcgaggac acccacaaga tggccgagtc gaacaaggcc
     8701 ttcgcgcact accgctggta gtccgcagcc ggtcgagtag cgcgtgagcg cgtatcgaga
     8761 ccaagtttcg atacgcgctg cgcgctactc aaccagccca gaatccaaca ccctctgaaa
     8821 gtaggaacac cgtggcacag gacgtgctca ccgacctgaa caaggtccgc aacatcggca
     8881 tcatggctca catcgacgcc ggcaagacga cgacgaccga gcgcatcctg ttctacacgg
     8941 gtatcaccca caagatcggt gaggtgcacg acggcgccgc gacgatggac tggatggcgc
     9001 aggagcagga gcgcggcatc acgatcacgt ccgccgcgac gacctgtttc tgggacaagc
     9061 accagatcaa catcatcgac acccccggcc acgtggactt caccgtcgag gtggagcgct
     9121 cgctccgcgt gctcgatggc gcggtcgccg tgttcgacgg caaggagggc gttgagcccc
     9181 agtccgagac cgtctggcgc caggccgaca agtacgacgt tcctcgcatc tgcttcgtca
     9241 acaagatgga caagctgggc gcggacttct acttcaccgt cgacacgatc atcaagcgcc
     9301 tcggcgccaa gccgctgg
//
LOCUS       DAOSOU010000068         9265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7396_length_9265_cov_6.331813, whole genome shotgun sequence.
ACCESSION   DAOSOU010000068 DAOSOU010000000
VERSION     DAOSOU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7396_length_9265_cov_6.331813"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLW15_05320"
     CDS             complement(<1..415)
                     /locus_tag="PLW15_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33855.1"
                     /translation="MDEKRLTDALQRLRAHAYARPPQPSRQLSDRLDLGMLADPPPVN
                     SPAQVAVVVAAPERRGRLRAVLSWVAGLGIVTKIVLGIGVAAIATTGVGAAGALPAPA
                     QQLFDSVLPGIAPHSSETADDPTDQPGDDTPGTTEP"
     gene            complement(456..1010)
                     /locus_tag="PLW15_05325"
     CDS             complement(456..1010)
                     /locus_tag="PLW15_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT33856.1"
                     /translation="MGGSGENVGGYAFDHVLIAAQQGAPWANTLLWAEYAPAVKAFLT
                     ARGSEEPDDLTSEVFLAVFDGLQAFSGDESQFRSFVFTIAYRRLVDEFRRRSRRGIHS
                     EWSPDSDGRAAASAEDEALTRVGDAAARMLLDGLPEDQRNVMVLRIVADLTVEQVAQV
                     LGKRPGAVKALQRRALDSLRRKLS"
     gene            1075..2463
                     /locus_tag="PLW15_05330"
     CDS             1075..2463
                     /locus_tag="PLW15_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT33857.1"
                     /translation="MPLEPDDYTAPLDRAAAHARQWLNTLPTRAIRPSATADDMLAAF
                     GGRLADHPTDAAQVVDELALRADPGLMAIGSGRFFGWVMGGTLPAALGADWLVSAWDQ
                     NSGMRYATPAAAAIEEAAGAWILDLLGLPAGCDVGFATGGTMANFVGLAAGRGDVLTR
                     AGWDLNSKGLIGAPPVHVLAGAEVHASVDLALRYLGLGLPTLVEADDEGRMRPEALAE
                     ALGLVSGPVIVVLQAGNLHSGAFDPMRELITIAHDHGAWVHVDGAFGLWAAASPQWAE
                     HLDGIELADSWATDAHKTLNVPYDCGVAIVARPEAARAALGVKTSYLIQDAGAPADPF
                     ELVPEMSRRGRGIPVWAALRQLGRSGTIAMVERLAATARALADGLDALPGVEVLNEVV
                     FTQVSLAFGSDERTRAVTQALIADGAVWMSGSKWHGRDILRISVSNWSTDDDDIATAI
                     DAVARALAAVPE"
     gene            complement(2464..3216)
                     /gene="yidC"
                     /locus_tag="PLW15_05335"
     CDS             complement(2464..3216)
                     /gene="yidC"
                     /locus_tag="PLW15_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOT33858.1"
                     /translation="MDIFAFPPIAALLEGAYWLVQFLSSLVHPLVGTAAAAVAIVLLT
                     VLVRAVLIPVSASQVKAEWGRRRIAPRLQALQKRYKKNPELLQRKTLELYRSENVSMF
                     GGFLPTLLLQAPVLSLVYALFLHSTIGGHPNDLLTERLGSVPLGSTLWQAGWPGVLVF
                     VVLFCAMGIVAWFSRRTALRLALPSEGQSTLLRALSWAPFLTIVFAAFVPLAATIYLA
                     TTTLWTLIERALLRRRHWGESGSAVGAVLTSS"
     gene            complement(3287..3580)
                     /locus_tag="PLW15_05340"
     CDS             complement(3287..3580)
                     /locus_tag="PLW15_05340"
                     /inference="COORDINATES: protein motif:HMM:NF039759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6412 domain-containing protein"
                     /protein_id="HOT33859.1"
                     /translation="MRLLTGRFVALTAMLLMAFALAQGATPAAVVASVAAVVLATAGA
                     IRLTSVIVGSRQVSVGEQAHARHLIQLGMPAPQHPDTAGRPRTRAPSMVVATT"
     gene            complement(3607..4035)
                     /locus_tag="PLW15_05345"
     CDS             complement(3607..4035)
                     /locus_tag="PLW15_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33860.1"
                     /translation="MIHDSAHNRRGRIGSIAAASVALIAVLTGCSPTGPITPVVTSAG
                     LDPTLPGYMQATGSVSGVSEDGGKCVFTFWADSGAATRLTGPGQATGLRTDCGPVKEA
                     VGFLVGGTYQVELTYEALSGETVKGARVPMVLPVPTNLNP"
     gene            4127..4483
                     /locus_tag="PLW15_05350"
     CDS             4127..4483
                     /locus_tag="PLW15_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT33861.1"
                     /translation="MPESLEREPLSSDLLRGHTDTIVLGVLLKVDRYGFEIYKTIRDA
                     TGGDYEIKEATLYATFRRLVKDGLVEAYWGDETQGGRRKYYRITDAGRAIYRRNLADW
                     TATQRIINSLLNLKGA"
     gene            4486..5364
                     /locus_tag="PLW15_05355"
     CDS             4486..5364
                     /locus_tag="PLW15_05355"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HOT33862.1"
                     /translation="MSTVTSIHRYLDEAFAEVPVTPDPLDLKEELRSNLSARVAELVS
                     GGADDATAATTAVRELGDIRDLLDGLAADGHPAGVPHPSQRVRLRPIFVLLTVLFSLV
                     LAKSVTWVVLGAFGIVPMWTAVAFAVAAALSVGVIVTLALRQETSQHYPMPTGRALAF
                     GAASLAMTLGVSLAAALVTQQPFWAVVLGVPPTVLSILAFVWLGVTQTNRTKPWALEL
                     QRQYVADDPFTQNPAVAARFGIYTVVIWTVAIAVFIVLTMTVGWLWSWLALVGGFAVF
                     FITLARMMLIARQSRV"
     gene            5408..5680
                     /locus_tag="PLW15_05360"
     CDS             5408..5680
                     /locus_tag="PLW15_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HOT33863.1"
                     /translation="MCRNIHTLHNFEPAANADEVNAAALQYVRKISGSTKPSKANQEA
                     FDRAVHEIAHITQHLLEDLVATTPPKDREVEAAKARERSAKRFATA"
     assembly_gap    5692..5791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5793..6812)
                     /locus_tag="PLW15_05365"
     CDS             complement(5793..6812)
                     /locus_tag="PLW15_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT33864.1"
                     /translation="MTTPRPWISRKRHWIPLALLAAGVSFAVVATLSPWPAALLIRAL
                     FEQSAAKTVAEMQPYVPASGLDAQLDVAYADGGADTTFDLFSPSGSTEPLPTVVWIHG
                     GAWISGSSRNVDPYVQILASKGYTTVALNYTVAPDATYPVALTQLNDALAFLVAHADE
                     YRIDPARIVIAGDSAGSQYTSQLATMITSPVYAEKVGLTPALSPDQLRAVILTCGIYD
                     VSGIPDAPGLGGWGFRVSLWAYLGTKDWSNTPGGELMSTLDDVTADFPTTWITGGNGD
                     PLTATQSMPLAEKLSSLGVDVTTVFYPDDHVPSLPHEYQFHLDDADAQAALTSMEAFL
                     GKVTG"
     gene            6840..7739
                     /locus_tag="PLW15_05370"
     CDS             6840..7739
                     /locus_tag="PLW15_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT33865.1"
                     /translation="MSPRHSLLALLVVVIWGANFVIIDAGLVDVPPLVFLALRFVAVC
                     IPAIFFIKPPTIGWRNILLIGSFLSLGQFALLYLALALGMPPGLASLILQTQVIFSIV
                     ISALFLRERPSRRQAIGVLIGMLGLVLVIVGHSASAPWLPLLVVLGSALSWAIGNVLS
                     RRSKAASGLGLVVWSGAVVPLPALALAVIVESPPVVWDALTHLSIAAILSTLYTAIAA
                     SLIGYGIWNTLLARYPTSAVVPFTLIVPVVGIVAAWLVQGEVPGALELIGGVVMLSGL
                     AAAVVQWRRRPTAVAAPPPYEES"
     gene            7759..8883
                     /locus_tag="PLW15_05375"
     CDS             7759..8883
                     /locus_tag="PLW15_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT33866.1"
                     /translation="MTRSEPTLRPSRSLAQSTGTPTAATRRIQVNALQIGLLGTLGVG
                     IGLFILGAISALGTVLAYIGVAFFIALALEPVIRWATGRGVPRWVASLAIVVLGLVIG
                     ALVALAVVPTIIAQVSNIIRDAPAFAASLPSQPWVQWVTQNLGSWIDTNALTQQLVAF
                     ISNPAQWVTIGGGILAIGSGLGEAVTAVIVVTVLTLYFTLTLPLVMTKVYQAVPRSRR
                     AGFMSVTDEILQSVGKYVAGQLFLALCNAVITFIVVTIVGGPVPLLLALVAFICALIP
                     VVGPVIGTSIVVLATLPITLWGALIAGAIMLVYMQVEAYILSPKVMAKAVAVPGALVI
                     VAAIGGASLGGILGALVAIPVVAAGILIFERVVRPRQEAR"
     gene            complement(8884..>9265)
                     /locus_tag="PLW15_05380"
     CDS             complement(8884..>9265)
                     /locus_tag="PLW15_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33867.1"
                     /translation="LWVSDGNLARRVYERAGFVATGERDVVRGELMEERMRRPLEPVE
                     PRETPGWRSAGIVFDGEPIDVDGINPWKHSWVDTGEIVWMRHPSYPTQLHDMPMYLIA
                     AEGRYITFAAGELSNTVWGFYLPV"
BASE COUNT         1440 a         3100 c         3090 g         1535 t
ORIGIN      
        1 tgggctcagt ggtgccgggg gtgtcgtcgc ccggctggtc ggtcgggtca tccgctgtct
       61 cgctcgaatg cggcgcaatg ccggggagta cggagtcgaa cagctgttgg gcgggcgcgg
      121 gcagtgcgcc ggcggcgccg accccggttg tcgcgatagc ggcgacaccg atgccgagca
      181 cgatcttggt gacgatgccg agtccggcca cccacgacag gacggcacgc agtcggcctc
      241 gacgctctgg cgcggccact acgaccgcga cctgcgctgg gctgttcacg ggcggcgggt
      301 cggcgagcat ccccaggtcg aggcgatccg atagctgacg cgacggctgc ggcggccgcg
      361 cgtacgcgtg cgcccgcaac cgctgcaggg cgtcggtcag ccgtttctcg tccatcactg
      421 atcgctatcg ctgaaccagc agaaaagggt acgggtcagg acaactttct tcgcaacgag
      481 tcgagcgccc gccgctggag tgctttcacc gcacccggtc gttttcccag aacctgagcc
      541 acctgctcga cggtgaggtc ggcgacgatg cgcagcacca tcacgttgcg ctgatcctcc
      601 ggcaagccat cgagcagcat tctggccgcg gcatccccca cccgggtgag agcctcgtcc
      661 tcggcactcg cggccgcgcg gccatcggag tcgggcgacc actcggagtg gatgcctcga
      721 cgcgagcggc gacgaaactc atcgaccagg cgtcggtagg cgatcgtgaa gacgaatgat
      781 cggaattggg actcgtcgcc ggagaacgcc tgcagcccgt cgaacaccgc gagaaacacc
      841 tcgctggtca ggtcgtccgg ctcctctgac ccgcgcgcgg tcaggaacgc cttcaccgcg
      901 ggcgcgtact cggcccacag aagggtgttg gcccatggcg caccctgctg ggctgcgatg
      961 agaacgtggt cgaacgcgta tccacccacg ttctccccag atccgcccac gcggcgatcg
     1021 tatcgggcca acgccggggg tgcggtcggc gaaccgccgg agtaccgtcg agccatgcca
     1081 ctggagccag acgactacac cgcgcccctc gaccgtgcgg ccgctcatgc caggcagtgg
     1141 ctgaatacgc tgccgacgcg cgcgattcga cccagtgcca ccgccgatga catgctcgcg
     1201 gcgttcggcg gcagactcgc cgaccacccc accgacgcgg cgcaggtcgt cgacgagctc
     1261 gcactccgcg cggatccggg tctcatggcg atcggatccg gacggttctt cggctgggtt
     1321 atgggcggca ccctgcccgc ggcgctcggc gccgactggc tcgtcagcgc ctgggaccag
     1381 aactccggca tgcgctacgc gacaccggcg gccgccgcga tcgaggaggc ggccggcgcg
     1441 tggatcctcg acctgctcgg cctgcccgcc ggatgcgacg tcggcttcgc gaccggaggc
     1501 acgatggcca acttcgtggg ccttgccgcc gggcgcggcg acgtgctcac gagagcaggc
     1561 tgggacctca acagcaaggg cttgatcggt gccccgccgg tgcacgtgct cgcgggcgcc
     1621 gaggtgcacg cgagcgtgga tctcgcgctg cgctacctcg ggctcgggct gcccacgctg
     1681 gtcgaggccg acgacgaggg ccggatgcgc cccgaggccc tcgccgaggc gctcggcctc
     1741 gtgagcggcc ccgtgatcgt cgtgctgcag gccggcaacc tccactccgg tgcgttcgac
     1801 ccgatgcgcg aactcatcac gatcgcccac gaccacggtg cctgggtgca cgtggatggc
     1861 gcattcggcc tgtgggcagc ggcgagcccg cagtgggccg agcatctcga cggcatcgag
     1921 ctggccgact cctgggcaac cgacgcccac aagactctca acgtgccgta cgactgcggg
     1981 gtcgcgatag tcgcgcgccc ggaggctgcg cgtgcggcct tgggcgtgaa gaccagctac
     2041 ctgatccagg atgcgggcgc gccggcggat cccttcgagc tggtgcccga gatgtcgcgg
     2101 cgtggtcgcg gcatcccagt gtgggcggcg ctgcggcaac tcggcagatc cggcaccatc
     2161 gccatggtcg agcgcctcgc ggcaaccgcc cgcgccctcg ccgacggcct cgacgccctt
     2221 ccgggagtcg aggtgctcaa cgaggtggtg ttcacgcagg tgtcgctcgc cttcggaagc
     2281 gacgagcgca cccgcgcggt gactcaggca ctgatcgcgg acggagccgt gtggatgtcg
     2341 ggctcgaagt ggcatggccg cgacatcctg cgcatctcgg tcagcaactg gtcgacggat
     2401 gacgacgaca tcgccaccgc gatagacgct gtcgcgcgtg cgctcgccgc tgtgcccgag
     2461 taattacgac gacgtcagca ccgccccgac ggcggagccg gactcccccc agtgccgtcg
     2521 ccgcagcagt gccctctcga tgagcgtcca gagggttgtc gtcgcgaggt agatcgtcgc
     2581 cgcgagcggc acgaaggcgg cgaacacgat ggtgaggaag ggcgcccaac tgagtgctcg
     2641 cagcagcgtc gactgaccct cgctcggcag agcgagccgt agcgccgttc gccgggagaa
     2701 ccacgcgacg atccccatcg cgcagaacag cacaacgaac acgagcactc cgggccaacc
     2761 cgcctgccac agcgtcgagc cgagcggcac cgaccccagc cgttcggtca gcagatcgtt
     2821 gggatgcccg ccgatcgtcg agtgcaggaa cagggcatac acgagcgaga gcacgggagc
     2881 ctgaagcagc agggtcggca ggaatccccc gaacatcgac acgttctcgg atcggtagag
     2941 ctcgagggtc ttgcgctgca gcagttccgg attcttcttg tatctcttct gcagggcctg
     3001 aagccgcggt gcgatacggc ggcgacccca ctcggctttc acctgggagg ccgacaccgg
     3061 aatgagcacc gcccgcacca gcacggtgag aagcacgatg gcgacagccg ccgccgcggt
     3121 gccgacaagc ggatgaacga gcgaggagag gaactggacg agccagtagg cgccctcgag
     3181 gagcgcggcg atagggggaa acgcaaagat gtccacggaa gaagtccttc gatctgaaac
     3241 ggatgggtgg tgggtaccac gcagcatccg ctcagtgcgg ttcggactac gtggtggcga
     3301 ccaccatcga aggggctcgg gtgcgggggc gacccgcggt gtcgggatgc tgcggcgcgg
     3361 gcatcccgag ctggatgagg tggcgcgcgt gggcctgctc gccgacggag acctggcgcg
     3421 acccgacgat caccgaggtc aggcggatcg cgcccgcggt ggcgagcacg actgcggcga
     3481 ccgaggcgac cacggcggcg ggagtggctc cctgcgccag ggcgaacgcc atgagcagca
     3541 tcgcggtgag cgcgacgaat ctgccggtga gcaacctcat ggctcgatgc tactcccggc
     3601 ggaaagctac gggttcaggt tggtcggcac cggaaggacc atcgggacgc gcgcgccctt
     3661 gaccgtctcg cccgagagcg cctcgtacgt gagttccacc tggtaggtgc cgccgacgag
     3721 gaagccgacg gcctccttca cgggaccaca gtcggtgcgc agtccggtcg cctgaccggg
     3781 gccagtgagt ctggtggctg cgccgctgtc tgcccagaag gtgaagacgc acttgccacc
     3841 gtcctccgag actcccgaga cgcttccggt cgcctgcatg tacccgggca gcgtggggtc
     3901 gagcccggca gacgtcacga ccggcgtgat cggcccggtg ggcgagcatc cggtcagcac
     3961 tgcgatgaga gcgacggatg ccgcggcgat actgccgatc cggcccctgc gattatgggc
     4021 gctgtcgtga atcatgctcg ctattgtgcc agtcgttggg gctggtcgcc aatcgggcag
     4081 catcaggttc ttgccgtgaa ctactgtgcc tgatacagta cttgacatgc ccgagtccct
     4141 cgaacgcgaa ccgctctcct ccgaccttct ccgggggcac accgacacga tcgtgctcgg
     4201 cgtgctgctg aaggtcgatc ggtacggttt cgagatctac aagaccatcc gtgatgccac
     4261 gggcggcgac tacgagatca aagaagcgac gctctacgca accttccgcc gcctcgtcaa
     4321 ggacggactc gtcgaggcct actggggcga cgagacccag ggcggccgcc gcaagtacta
     4381 ccgcatcacc gacgccggcc gggccatcta ccggcgcaat ctcgccgact ggaccgcaac
     4441 ccagcgcatc atcaactccc tcctgaacct gaaaggcgcg tgaccatgag caccgtcacc
     4501 tccatccacc gctacctcga cgaggccttc gccgaagttc ccgtcacccc agacccgctc
     4561 gacctcaagg aggagctgcg ctccaacctg tcggcccgcg tcgcagaact tgtgtccggc
     4621 ggcgcggatg acgcgaccgc agccaccacc gcggtgcgcg agctcggcga catccgtgac
     4681 ctgctcgacg gtctcgcggc cgatggacac cccgccggcg tgccgcaccc cagccagcgg
     4741 gtgcggctcc gtccgatctt cgtgctgctg acggtgctgt tctcgctggt tcttgcgaag
     4801 agcgtgacgt gggtggtgct cggcgcgttc ggcatcgttc cgatgtggac ggctgttgcc
     4861 ttcgccgttg ccgcagcgct ctcggtcgga gtcattgtga cgctcgcctt gcgccaggag
     4921 acctcgcagc actacccgat gcccaccggc cgggcgctcg ccttcggagc ggcatccctg
     4981 gccatgacac tgggtgtttc cctcgcagcc gcgctcgtca cccagcagcc gttctgggcg
     5041 gtcgtactcg gtgtgcctcc gacggtgttg tcgattctcg ccttcgtgtg gctcggagtc
     5101 acccagacca atcgcacgaa gccgtgggcg cttgagctgc agcgccagta cgtggctgat
     5161 gaccccttca cgcagaaccc tgcggttgcg gcgcgattcg gcatctacac ggtcgtcatt
     5221 tggacggttg cgattgctgt cttcatcgtg ctcaccatga ccgtcggctg gctctggtcg
     5281 tggctggcac tcgtcggcgg attcgcggtg ttcttcatca cgctcgctcg catgatgctc
     5341 atcgcccgtc agagtcgggt ctgacatagg ggtgtggatg tcgcggcgcg cagctagcct
     5401 cgatggcatg tgccggaaca tccacaccct tcacaacttc gaacccgccg cgaacgccga
     5461 cgaggtgaac gcggccgctc tgcagtacgt acgcaagatc agcggcagca cgaagccgtc
     5521 caaggccaac caggaagcgt tcgatcgtgc cgtgcacgag atcgcccaca tcacccagca
     5581 ccttctcgag gacctcgtgg ccaccactcc cccgaaggac cgcgaagtcg aggcggccaa
     5641 ggcgcgcgag cgctccgcga agcgattcgc gaccgcctga tcgctagccc gnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctagcccg tcaccttgcc caggaaggcc
     5821 tccatcgacg tcagcgcggc ctgggcgtcg gcgtcatcga ggtggaactg gtactcgtgc
     5881 ggcagtgatg gcacgtggtc gtcgggatag aagacggtcg tgacatccac gccgaggctt
     5941 gacagcttct cggcgagcgg catcgactgc gtggcggtga gcggatcgcc gttgccgccc
     6001 gtgatccagg tggtgggaaa gtccgccgtc acatcgtcga gcgtcgacat cagttcgcca
     6061 ccgggggtgt tggaccagtc cttggttccc aggtaggccc agagcgagac tcggaatccc
     6121 cagccgccca ggccgggcgc atcggggatg ccgctcacgt cgtagatgcc gcaggtgagg
     6181 atcaccgcgc gcaactgatc ggggctcagc gccggcgtca accccacctt ctcggcgtag
     6241 accggactcg tgatcatcgt cgccagctgg ctcgtgtact gggagcccgc cgagtcaccg
     6301 gcgatcacga tgcgggcggg atcgatgcgg tactcatcgg cgtgcgcgac caggaaggcg
     6361 agcgcatcat tgagctgggt gagcgccacg gggtaggtcg catcgggagc gaccgtgtag
     6421 ttcagggcca cggtcgtgta gcccttcgat gcgaggatct gcacgtaggg gtcaacgttg
     6481 cgactgctgc cggagatcca ggcaccgccg tgaatccaga cgaccgtggg cagcggctcg
     6541 gttgagcccg acggcgagaa caggtcgaag gtcgtgtctg cgccgccgtc ggcgtaggcg
     6601 acatccagct gggcatcaag accggatgcc ggaacatacg gctgcatctc ggcgaccgtc
     6661 ttggcggcgc tctgctcgaa cagcgcacgg atcagcaggg ccgcgggcca ggggctcagg
     6721 gtcgcgacca cggcgaagct gacacccgcc gcaagcaggg cgagcggaat ccagtggcgc
     6781 ttgcgcgaga tccaggggcg cggggtcgtc atgctgatga cgatagtgtg aggatgccca
     6841 tgagcccacg acattcactc ctcgcgcttc ttgttgtggt gatctggggt gccaacttcg
     6901 tgatcatcga tgccgggctg gtcgacgttc ccccgctggt gttcctggcc ctgcgattcg
     6961 tcgccgtgtg catcccggcc atcttcttca tcaagccgcc gacgatcggg tggcgcaaca
     7021 tcctgctgat cggctcgttc ctcagcctcg gccagttcgc cctgctctac ctcgccctcg
     7081 ccctcgggat gccgcccggg ttggcgtcac tcatcctgca gacccaggtg atcttctcca
     7141 tcgtgatctc ggcgttgttc ctgcgggagc gcccatcgcg acggcaagcc atcggtgtgc
     7201 tgatcgggat gctcggcctc gtgctcgtca tcgtcgggca cagtgcgtca gcaccctggc
     7261 tgccgctgct ggttgtgctc ggctcggcgc tgtcgtgggc gatcggcaac gtgctgtccc
     7321 gccgttcgaa ggcggcatcc ggactcgggc tggtcgtgtg gagcggtgcc gtcgtgcccc
     7381 tgccggcgct agccctggcg gtgatcgtgg agtcaccgcc agtggtctgg gatgccctca
     7441 cccacctctc gatcgcagcg atcctctcga ctctgtacac cgcaatcgcc gcatcgctga
     7501 tcggctacgg catctggaac accctgctcg cgcgctatcc gaccagcgcc gtcgtgcctt
     7561 tcacgctgat cgtgcccgtg gtcggaatcg tcgcggcgtg gctggtgcag ggcgaggtac
     7621 cgggcgcgct ggagttgatc ggcggtgtgg tgatgctttc tggtctcgca gcagcggttg
     7681 tgcaatggcg gaggcgcccg acagcggtcg ccgcgcctcc cccctacgag gagagttgat
     7741 cgctagtctc gcctcacgat gacacgatcc gaaccgaccc tcaggccatc gcgatccctc
     7801 gcgcagtcga ccggcacgcc cacggcggcg acccggcgca tccaggtcaa tgccctccag
     7861 atcggactgc tgggcaccct cggcgtgggc atcggactgt tcatcctcgg cgccatcagt
     7921 gcactcggca cggtgctcgc ctacatcggg gtggcgttct tcatcgcgct cgcgctggag
     7981 cccgtgatcc gctgggccac ggggcgaggg gtgccccgct gggtggcatc gctggcgatc
     8041 gtcgtgctcg gtctcgtgat cggcgcgctc gttgcgctgg cggtcgtgcc gaccatcatc
     8101 gcccaggtga gcaacatcat ccgggatgcc cccgccttcg ccgcgagcct gccgagtcaa
     8161 ccgtgggtgc agtgggtcac ccagaacctc ggcagttgga tcgacacgaa cgcgctcacc
     8221 cagcaattgg tcgccttcat cagcaacccc gcccagtggg tcaccatcgg cggcgggatc
     8281 ctggcgatcg gttcaggcct cggcgaggcc gtcaccgcgg tgatcgtcgt caccgtattg
     8341 accctgtact tcaccctcac tctgcccctg gtgatgacga aggtgtacca ggcggtgccg
     8401 cggtcgcgtc gtgccggctt catgtcggtc accgatgaga tcctccagtc ggtcggcaag
     8461 tatgtggccg ggcagctgtt cctcgctctg tgcaatgcgg tcatcacgtt catcgtggtg
     8521 acgatcgtgg ggggaccggt tcctctgctc ctcgcgctcg tcgcattcat ctgtgcgctc
     8581 atccccgtcg tcggccccgt cattgggacc tcgatcgtcg tgctcgccac tctgcccatc
     8641 actctctggg gtgcgctgat cgcaggagcg atcatgctcg tctacatgca ggtggaggca
     8701 tacatcctga gccccaaggt catggccaaa gccgtggcgg tgccgggggc gctcgtgatc
     8761 gttgccgcta tcgggggtgc atcgctgggc ggaatcctcg gcgcgctcgt cgcgattccg
     8821 gtcgtcgcgg cgggcatcct gatcttcgag cgcgtggtgc ggcccagaca ggaagcccgg
     8881 tagtcagacg gggaggtaga agccccacac ggtgtttgac agctcacccg cggcgaaggt
     8941 gatgtaacga ccctcggccg ctatcagata catcggcatg tcgtgcagct gcgtcgggta
     9001 cgacgggtgc cgcatccaga cgatctcgcc ggtgtcgacc caggagtgct tccacgggtt
     9061 gatcccgtcg acgtcgatgg gctcgccgtc gaacacgatt ccagcggagc gccagcccgg
     9121 agtttcgcgc ggctcgaccg gttcgagtgg gcggcgcatc cgctcctcca tgagctcgcc
     9181 gcgcaccacg tcgcgctcgc ccgttgcaac gaagcccgcg cgttcgtaga cgcgacgggc
     9241 gaggttgccg tcggagaccc acagc
//
LOCUS       DAOSOU010000069         9029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7673_length_9029_cov_7.309338, whole genome shotgun sequence.
ACCESSION   DAOSOU010000069 DAOSOU010000000
VERSION     DAOSOU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7673_length_9029_cov_7.309338"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1400)
                     /locus_tag="PLW15_05385"
     CDS             complement(138..1400)
                     /locus_tag="PLW15_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT33868.1"
                     /translation="MTTTTDLAPGQLPNEVAPPSRAQQVLRDIMGGSAVISVLAVVLA
                     LLAGAILIVLTDESVQQASGYFFSRPGDTFKAIWDAVAGAYSALFQGSIYNFRVDGFA
                     KGIKPFTETLTFATPLIAAGLGVAVGFRAGLFNIGGRGQMLVAAGAAGYVGFAWPLPF
                     GIHLIVAVVLGMLAGAIWGGIAGLLKARTGAHEVIVTIMLNYVGFYLISFLLHTPGAL
                     QAPGSSNPKSPAMLETAIFPKLLGPSYNLHLGFVFAIIATVFVWYLINRSSLGFKFRA
                     VGANPNAALVAGISVKNVYLYAMLISGALVGLAGVSQVLGTVTTGFTGGIDAGIGFDA
                     ITVALLGRSKPVGVFIAGVLFGALKAGGYSMQAAQGIPIDIVLVVQSLIVLFIAAPPL
                     VRAIFRLPVPGTTSKRAAKLAATKAVAE"
     gene            complement(1397..2917)
                     /locus_tag="PLW15_05390"
     CDS             complement(1397..2917)
                     /locus_tag="PLW15_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33869.1"
                     /translation="MKLELRGITKRFGSLVANDHIDLTVEPGEIHALLGENGAGKSTL
                     MNVLYGLYQADEGQILLDDEVQHFAGPGDAMNAGIGMVHQHFMLIPVFTVAENVMLGH
                     EETKFGGRLDLAGARQKVREISARFGFDVDPDALVEDLPVGVQQRVEIIKSLSRDAKV
                     LVFDEPTAVLTPQETDELMVIMRQLKESGTSIVFITHKLREVREVADRITVIRLGKVV
                     GEASPTATNEELASLMVGRAVDLMIHKGPAHPGATALVVENLSVIDQRNQLVVNNVSF
                     EVHEGEVLAIAGVQGNGQTELTEAIMGLQHRITGNMHLHGVSLRELSVRKTLDAGVGF
                     VPEDRTEDGLIAEFTISENLMLDRATQPPFVKAGTLQLKYLAEFATAKVAEFDVRTQS
                     INTPVGKLSGGNQQKVVLARELSRDLKLFVAAQPTRGLDVGSIEFVHTRIIQARDAGV
                     PVIVVSTELDEVVALADRIAVMYRGGIVGIVPGDTPRDVLGLMMAGETPAQAGAAA"
     gene            complement(3024..4163)
                     /locus_tag="PLW15_05395"
     CDS             complement(3024..4163)
                     /locus_tag="PLW15_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT33870.1"
                     /translation="MSRHFLAGSILEDTLRITSRGRVLSGIALIGTSALVLAGCAAAP
                     ETGGETAAPSDFVPCMVSDSGGFDDKSFNQLGYEGLTEAADSLGVTPITVESASETDY
                     APNIDSLVSQGCTLIFTVGFALSAATLTAAEANPDIQFALIDDAADADFNGTPDFDNT
                     KPIVFNTAQAAFLAGYAAASYSKSGIVATWGGMNFPTVTIFMDGFAQGVAYYNQAMGK
                     SVTVLGFDPASPDTATMTGGFEANDVAKSTAQNFIDQGADVLLPVGGPIYQSAVAAIA
                     DSGKDIALVGVDADLFVTDPSTADIILTSILKGIKQATFDVVTGAAGGDFSNAPYVGT
                     LENDGVGIAPFHNFESKVSPDLQGELDKIKAGIIDGSIPVASYLG"
     gene            complement(4298..5419)
                     /locus_tag="PLW15_05400"
     CDS             complement(4298..5419)
                     /locus_tag="PLW15_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOT33871.1"
                     /translation="MSRSAASVPGFYSVIPAGGIGSRLWPLSRADAPKFLHDLTGSGQ
                     TLLRDTWDRLAPLSGSDRIMVVTGRAHRAAVEAQLPDLTDPNVVLESEPKDSSAAIGL
                     AAAILVSREPDVIIGSFAADHVITDVRGFRRAVEEGVAAARAGYIATIGITPTEPAIG
                     FGYIHCAGPLAIDGAPHAVAVESFVEKPDLATAEKYVADGNHLWNGGMFIARADVLLA
                     QLGESNPRLLEGLTELAAAWDTPRRGAVVDKVWPALPKIAIDYTVAEPAAASGKLVVI
                     PGDFDWDDVGDFASIAKLHAGGRKSDLAILGENARVLADSSTGVVISQSGRLISLIGV
                     TDIVVVDTPDALLVTTTANAQRVKSVVDALKVSGRDDVL"
     gene            5508..6635
                     /locus_tag="PLW15_05405"
     CDS             5508..6635
                     /locus_tag="PLW15_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT33872.1"
                     /translation="MRVVVVSESFLPTTNGVTTSVRKVLDHLAAEGHEAIVIVPTAGA
                     PATYAGFRVHEVPAIAYRQFPVGLPHPMVQKLIADFKPDVVHAASPFLLGAQAIAAAG
                     RLGIASVAIFQTDVAGYARRNRLGAATRFAWRIVRWIHEGADLTLVPSSASMADLEGA
                     GVQRLARWGRGVDLDTYHPRNRTAPGVRRLRERLAPHGETIVGYVGRLAPEKQVERMS
                     ALRGLSGIRLALVGSGPSLGSIKRELSGMPVTYLGPLSGPDLAAAYASFDVFLHTGTE
                     ETFGQTVQEAHASGLPVVAPRAGGPIDLIDHGTTGYLYDPEDAATLRPYVEALTIDPE
                     LRSRMGEAGRRGVLGKTWHGVCSDLVGYYEQAIASRVVVAG"
     gene            complement(6660..7589)
                     /locus_tag="PLW15_05410"
     CDS             complement(6660..7589)
                     /locus_tag="PLW15_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33873.1"
                     /translation="MRPSLAVCALLVPVLVLGGGVAALASTTDDYEAHRVASAEYDHA
                     LADAADVRTSASLSLDHYIAIAAEAKVALATGADLQAVGSDLLDATATAALGPLRDAL
                     TAALPDPGPTLEARPAVVHDSDPAVLTDATAELTEWSGDLRDEWSGHDATIAEIDDAL
                     HRLRTGIMAVAGSVAARGAGVLDASGSASSETRAALSSAIARVGGHIEDGTDPSADLT
                     AYLGAAKAVQDSHAEAIAAAAAGSGSGGVDLASGYVPYMGYQEGSYEWAYWLCHGFPW
                     NLPPGMGPPGPIPADYYPCTVFNLDGSTWVFYY"
     gene            7825..8244
                     /gene="sdhC"
                     /locus_tag="PLW15_05415"
     CDS             7825..8244
                     /gene="sdhC"
                     /locus_tag="PLW15_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOT33874.1"
                     /translation="MASEPPATPVIRNPKRPAGTLYRGREGMWGWVLHRITGTAIYFF
                     LLVHILDTSLVRLSPEAYNAVINTYKTPIMGLGEIALVAAIGLHALNGLRIILIDFSS
                     WGTKHQRLMFWIVVGLWLVLLAGFVPRQLMHIFGGAA"
     gene            8241..8684
                     /locus_tag="PLW15_05420"
     CDS             8241..8684
                     /locus_tag="PLW15_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HOT33875.1"
                     /translation="MSVIDAPRSKAAPKRRGINWEKWGWIYMRASGVVLVVLIFGHLF
                     VNLVAGEGVKAIDFAFVAGKWADPFWVVWDTLLLWLALIHGGNGMRTLVNDYANNKTV
                     RRILLAGVFASTAILIILGTLVIYTFDPCPVGAAPELLPSFCPVP"
     gene            8684..>9029
                     /locus_tag="PLW15_05425"
     CDS             8684..>9029
                     /locus_tag="PLW15_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOT33876.1"
                     /translation="MADQIADGTVIDGVHYHQHEIVIVGAGGAGMRAAIEAGPHASTA
                     VISKLYPTRSHTGAAQGGMAAALANVEEDNWEWHTYDTVKGGDYLVDQDAAEILAKEA
                     IDAVIDLENMGLP"
BASE COUNT         1576 a         2905 c         3044 g         1504 t
ORIGIN      
        1 gaacacgatg aggagcacga aggcgagcac cgagaagatc ccgaacgcga cgggtgcctt
       61 ccagttgcgc acgaccgctg tctcgagagc gaccctcggg gtcgagggca cgggcacctg
      121 ggctgctgtt gtcgaggtca ttcggccacc gccttggtcg cagcgagctt cgcggcacgc
      181 ttcgatgtgg tgcccggtac gggcagtctg aagatcgcgc gcacgagagg cggcgcagcg
      241 atgaagagca cgatgagcga ctggacgacg agaacgatgt cgatcgggat gccctgtgcc
      301 gcctgcatcg agtacccgcc ggctttcagc gcaccgaaca gcaccccggc gatgaacact
      361 cccaccggct tcgaacgccc gagcagggcc accgtgatcg cgtcgaatcc gatgcccgcg
      421 tcgattccgc cggtgaagcc ggtggtcacc gtgccgagca cctgcgagac gcctgcgagt
      481 ccgacgagtg cgccggagat cagcattgcg tagaggtaga cgttcttgac gctgattccg
      541 gcgacgagag ccgcattggg gttggcccca accgcccgga acttgaatcc gaggctcgac
      601 ctgttgatga ggtaccacac gaacaccgtg gcgatgatcg cgaagacgaa gcccaggtgc
      661 aggttgtacg acgggccgag cagcttcggg aagatggccg tctccagcat cgcgggagac
      721 ttcgggttgc tcgaccccgg cgcctgaagc gctccggggg tgtggagcag gaacgagatc
      781 aggtagaacc cgacgtagtt gagcatgatc gtcacgatca cctcgtgcgc gccggtgcgg
      841 gccttgagca ggccggcgat tccaccccag atcgcgccgg cgagcatccc gagcaccact
      901 gcgacgatca ggtggatgcc gaacggcagc ggccaggcga agcccacgta gccagcagct
      961 ccggccgcga ccagcatctg tccacgccca ccgatgttga acagtccggc gcggaagccg
     1021 acggcaacac ccagaccggc cgcgatcagc ggggtcgcga aggtcagcgt ctcggtgaag
     1081 ggcttgatgc ccttggcgaa gccgtccacc cggaagttgt agatcgaccc ctggaacagt
     1141 gcgctgtagg caccggcgac ggcgtcccag atggccttga aggtgtcacc gggccgcgag
     1201 aagaagtagc ccgaggcctg ctgcaccgac tcgtcggtga gcacgatgag gatggcgccg
     1261 gcgagcaggg cgagcaccac ggccagaacc gagatgacgg cactgccacc catgatgtcg
     1321 cggagcacct gctgggcgcg cgacggcggc gccacctcgt tgggcaactg gccgggggcc
     1381 aggtcggttg tggtcgtcat gccgcagctc ctgcctgggc cggggtctcc ccggccatca
     1441 tgagtccaag tacgtcgcgc ggagtgtcgc cgggaacgat cccgacgatg cctccgcggt
     1501 acatcaccgc gatccggtcc gcgagcgcga cgacctcgtc gagttcggtg gagaccacga
     1561 tcaccggcac gccggcatcc ctggcctgga tgatcctggt gtgcacgaac tcgatggatc
     1621 cgacgtcgag tccgcgggtc ggctgggcgg cgacgaacag cttgagatcg cggctcagct
     1681 cgcgcgcgag cacgaccttc tgctggttgc cgcccgacaa cttgccgacc ggcgtgttga
     1741 tcgactgcgt gcgaacgtcg aactcggcga ccttggcggt cgcgaactcg gccaggtact
     1801 tcagctgcag agtgcccgcc ttgacgaacg gtggctgggt cgcgcggtcc agcatgaggt
     1861 tctcggagat ggtgaactcc gcgatgaggc cgtcctcagt gcgatcctcg ggaacgaacc
     1921 cgacgccagc atcgagcgtc ttgcgtaccg agagctcgcg cagcgagacg ccgtgcaggt
     1981 gcatgttgcc ggtgatccgg tgctgcagac ccatgatcgc ctcggtgagc tcggtctggc
     2041 cgttgccctg aaccccggcg atggcgagaa cctcgccctc gtgcacctcg aagctgacgt
     2101 tgttgacgac caactggttg cgctggtcga tgaccgacag gttctccacc acgagcgctg
     2161 tcgcccccgg atgcgccgga cccttgtgga tcatcaggtc gaccgcgcgg cccaccatca
     2221 gcgacgcgag ctcctcgttg gtcgccgtcg gagaggcttc gccgacgacc ttgcccaagc
     2281 ggatgaccgt gatgcggtcc gcgacctcgc gcacctcccg cagcttgtgg gtgatgaaca
     2341 cgatggaggt tcccgactcc ttgagctggc gcatgatgac catgagctcg tcggtctcct
     2401 ggggggtgag cactgcggtg ggctcgtcga acacgagcac cttcgcgtcg cgcgatagcg
     2461 acttgatgat ctcgacccgt tgctgcacgc cgacgggcag atcctcgacg agggcgtcgg
     2521 ggtcgacgtc gaacccgaac cgcgcggaga tctcgcggac cttctggcgc gcaccggcaa
     2581 ggtcgagacg gccaccgaac ttcgtctcct cgtggccgag catgacgttc tcggcgacgg
     2641 tgaatacggg gatcagcatg aagtgctggt gcaccattcc gatgcccgcg ttcatcgcgt
     2701 cgccggggcc ggcgaagtgc tggacctcgt cgtccagcag gatctggccc tcgtcggcct
     2761 ggtacagccc gtagagcaca ttcatcaggg tggacttgcc ggcgccgttc tcgcccagca
     2821 atgcgtggat ctcgcccggt tcgaccgtga ggtcaatgtg gtcgtttgcg accagggaac
     2881 cgaacctctt ggtgatcccg cgaagttcta gcttcatgtc gtccaatcta acgcggcggc
     2941 tctgccgatg gtgctgtcaa gttaagaagg tcggggagac cagctgaggc agctggcctc
     3001 cccgaccggg gtactacgag tgactagccg aggtagctgg ccaccgggat gctgccgtcg
     3061 atgatgccgg ccttgatctt gtcgagctca ccctgcaggt caggcgaaac cttcgactcg
     3121 aagttgtgga acggcgcgat accgactcca tcgttctcga gggttccgac gtacggggcg
     3181 ttggagaagt cgccaccggc cgcaccggtc accacgtcga acgtcgcctg cttgatgccc
     3241 ttgaggatcg aggtcaggat gatgtcggcg gtcgacgggt cggtcacgaa caggtcagcg
     3301 tcaacaccga cgagggcgat gtccttgccc gagtcagcga tggccgcgac ggcgctctgg
     3361 tagatcggtc caccgaccgg cagcagaaca tctgcaccct ggtcgatgaa gttctgagcg
     3421 gtgctcttgg cgacgtcgtt ggcctcgaag ccaccggtca tggtggcggt gtcggggctg
     3481 gccgggtcga aaccgaggac ggtgacggac ttgcccatcg cctggttgta gtaggcaaca
     3541 ccctgagcga agccatccat gaagatcgtg acggtgggga agttcatgcc accccaggtc
     3601 gcgacgattc cggacttgga gtagctcgca gcggcgtagc cggcgaggaa cgcggcctga
     3661 gccgtgttga acacgatcgg cttcgtgttg tcgaagtcgg gcgtgccgtt gaagtcggcg
     3721 tctgccgcgt catcgatcag cgcgaactgg atgtcggggt tggcctcagc cgccgtcagg
     3781 gtcgctgcgg agagcgcgaa gccaacggtg aagatgagcg tgcagccctg cgacacgagg
     3841 ctgtcgatgt tcggtgcgta gtcggtctcg gaagcggact cgacggtgat cggcgtgacg
     3901 ccgagcgagt cggctgcctc ggtgaggccc tcgtagccga gctggttgaa cgacttgtcg
     3961 tcgaaaccgc cggagtcaga gaccatgcag ggaacgaaat cgctgggagc ggcggtctcg
     4021 ccgccagttt cgggtgctgc tgcacaaccg gcgagaacga gcgcgctcgt gccgatcagt
     4081 gcgataccgc tgagtacgcg gccgcgagag gtgattctca aggtgtcctc caagatgctg
     4141 cccgccagga agtgacggga catagtgcaa tacacgttac cgaatgttac gaatcgcgca
     4201 tcagccaaac cgaggctgac gcacaaaggt tacaaactcg cgatgcttgg gaaatcagcg
     4261 ggaaatgttc agtacatttc ggggcggact gcacccgtta gagcacgtcg tcccggccgg
     4321 acaccttgag ggcatccacg actgacttga ctcgttgggc gttggccgtc gtggtcacga
     4381 gcagggcatc cggggtgtcg acgacgacga tatcggtcac tccgatgagc gagatgagcc
     4441 gaccactctg actgatcacg acgccggtgg acgagtcggc gagcacgcgc gcgttctccc
     4501 ccaggatcgc gaggtcgctc ttgcgaccgc cagcgtgcag cttggcgatc gaggcgaagt
     4561 cgccgacgtc atcccagtcg aagtcgcccg ggatgaccac gagtttgcct gacgccgcgg
     4621 cgggctcagc gacggtgtag tcgatcgcga tcttgggaag tgccggccac accttgtcga
     4681 cgaccgcgcc acgtcgaggc gtgtcccagg cggccgcgag ctccgtgagt ccctcgagca
     4741 gccgggggtt cgattcaccc aactgggcca acagcacgtc ggcgcgggcg atgaacattc
     4801 cgccgttcca gaggtgattg ccgtcggcga catacttctc ggctgtagcg aggtcgggct
     4861 tctcaacgaa tgactcgacg gcgacagcgt gcggggcgcc atcgatggcc aggggtccgg
     4921 cgcagtggat gtacccgaac ccgatcgccg gctccgtggg agtgatgccg atggtcgcga
     4981 tgtaaccagc cctggcggcc gccacgccct cctcgaccgc gcgacggaag cctctgacat
     5041 ccgtgatcac gtggtcggcc gcgaatgagc cgatgatgac gtcaggttcg cggctgacca
     5101 gaatcgctgc cgcgagcccg atcgccgcgg aggagtcctt cggctcgctc tcgagcacga
     5161 cgttgggatc ggtgaggtcg ggcagctgag cctctacggc ggcgcggtga gcgcgtccgg
     5221 tcacgaccat gatgcggtca ctccctgaca gcggtgcgag ccgatcccag gtgtcgcgca
     5281 acagagtctg ccctgagccg gtgaggtcgt gcaggaactt cggtgcgtcg gcacgcgaga
     5341 gcggccagag gcgtgatccg atgccacccg cggggatgac gctgtagaac cccggcacgc
     5401 tcgctgcgga tcgactcatg cctcaaacat agacttaagg ggacattcac ctgccggtca
     5461 cgtcgttctc ggcggtcccg tcacgtcacc ttggcatcgt gacagtcatg cgagttgtgg
     5521 ttgtcagtga gagtttcctc cccaccacca acggcgtcac caccagcgtg cggaaggtgc
     5581 tcgatcacct cgccgccgag ggacacgagg cgatagtcat cgttcccacc gctggcgcac
     5641 ccgcgaccta cgcgggattc cgagtgcacg aggtgccggc gatcgcgtac cggcaattcc
     5701 cggtcggcct gcctcacccg atggtgcaga agctgatcgc cgacttcaag cctgatgtgg
     5761 tgcacgcggc atccccgttc ctgctcgggg cacaggctat tgccgcagca ggtcgactcg
     5821 gcatcgcgag cgtcgccatc ttccagacgg atgtcgccgg ctacgcccgc aggaaccgac
     5881 tgggcgccgc gacccgcttc gcctggcgca tcgtgcgctg gatccacgag ggtgccgacc
     5941 tgactctcgt gccgtcgagc gcgtcaatgg ccgatctgga gggtgccgga gtgcagcgac
     6001 tcgcgcgctg gggccgcggc gtcgacctcg atacctacca cccgcgcaat cggactgctc
     6061 cgggagttcg tcggctgcgc gaacggctgg ctccgcacgg cgagaccatc gtggggtacg
     6121 tgggccggtt ggccccggag aagcaggtcg agcggatgag cgcgctgcgg gggctcagcg
     6181 gcatccgtct cgccttggtc ggcagcggac cgtcgctcgg ctcgatcaag cgagaactga
     6241 gcggcatgcc ggtgacctac ctcgggccac tatcgggccc ggacctggcc gcggcgtatg
     6301 ccagtttcga cgtgttcctg cacacgggga cggaggagac cttcggtcag accgtgcagg
     6361 aggctcatgc gtctgggctt ccggtcgtcg cccctcgagc gggcgggccg atcgatctca
     6421 ttgatcacgg tacgaccggc tacctctatg accccgagga tgccgcgacc ctgcgtccgt
     6481 acgtcgaagc cctcaccatc gaccccgagc tgcgctcacg catgggcgaa gccggccggc
     6541 gtggcgtact gggcaagacc tggcacggcg tgtgcagcga cctcgtcggg tactacgagc
     6601 aggccatcgc gtcgcgggtg gttgttgcgg gctgaggcgc gtctgccgac cccgtggagt
     6661 cagtagtaga acacccacgt cgatccgtca aggttgaaga cggtgcacgg gtagtagtcc
     6721 gccgggatcg gtccgggagg tcccattccc ggggggaggt tccagggaaa tccgtgacaa
     6781 agccaatagg cccattcgta ggatccctcc tggtacccca tgtagggaac gtagccactt
     6841 gctaggtcga caccgccact gccactcccg gccgcagccg cggcaatggc ctcagcgtgc
     6901 gagtcctgca ccgctttcgc agcgccgagg taggcggtga gatcggcgga cgggtcggtg
     6961 ccgtcctcga tgtgaccgcc gacgcgggcg atcgcggagg agagcgcagc gcgagtctcg
     7021 gacgaggctg agccggaggc atccagtacc ccggcgcccc tggcggcgac cgagcccgcg
     7081 acagccatga tcccggtgcg aagtcgatgg agggcatcat cgatctcggc gatcgtcgcg
     7141 tcatggcccg accactcgtc gcgcaggtca cccgaccatt cggtgagctc ggcggtcgcg
     7201 tcggtgagca cggccgggtc cgagtcgtgg acgactgccg gccgagcctc gagggtgggg
     7261 cccgggtcgg gtagcgccgc ggtcagggcg tcacgcaggg ggccgagcgc ggcggtggcc
     7321 gtggcatcca gcagatcgga accgacggcc tgcaggtcgg cgccggtcgc aagcgccacc
     7381 ttcgcctcag cagcgatcgc gatgtagtga tcgagcgaga gactggccga cgtgcgcacg
     7441 tcagcggcgt ccgcgagtgc gtgatcgtat tcggcggatg cgacgcggtg agcctcgtag
     7501 tcgtctgtag tcgacgccag cgccgccaca cctccgccca gtacgagcac cggcacgagc
     7561 agagcacaca ccgcgaggga tggccgcatc ggccctcctc ggtccgtaga tctgacgaat
     7621 acacagtatc gacgcgcgct caatcgggga accgtcgatt tatcttgaaa tcgagcgatc
     7681 tttcagcgtg actggacagt gcaccccgaa acacagtcat agactgaatg aggtccggcc
     7741 acggaccgtt cgtcggtcgc ctcgacgcga ccacttcagc caagcaagga ggggtcgctc
     7801 gtgccagaga agtcggcgag taccctggcg agcgaaccgc ccgccacacc cgtgatcaga
     7861 aacccgaaac ggccagcggg aacgctgtac cggggtcgcg aaggcatgtg gggctgggtg
     7921 ctgcaccgca tcacaggcac cgcgatctac ttcttcctcc tcgtgcacat tctcgacacc
     7981 tcgctggtgc ggctgagccc cgaggcctac aacgcggtga tcaacacgta caagacgccc
     8041 atcatgggcc tcggtgagat cgccctcgtg gcggcgatcg gcctgcacgc gctcaacgga
     8101 ctgcggatca tcctgatcga cttctccagc tggggcacca agcaccagcg cctcatgttc
     8161 tggatcgtcg tcggcctgtg gctcgtgctg ctggcaggct tcgttccccg ccagctgatg
     8221 cacatcttcg gaggtgccgc atgagtgtga tcgacgcacc ccgcagcaag gcagccccga
     8281 agcgccgcgg catcaactgg gagaagtggg gctggatcta catgcgcgcg agcggtgtcg
     8341 tgctcgtcgt gctgatcttc ggtcacctgt tcgtgaacct cgttgccggt gagggcgtca
     8401 aggccatcga cttcgccttc gtcgccggca agtgggccga tccgttctgg gtcgtctggg
     8461 acaccctgct gctctggctc gccctcatcc acggcggcaa cggcatgcgc accctcgtca
     8521 acgactacgc caacaacaag acagtgcgga ggatcctgct cgcgggagtc ttcgcatcca
     8581 ctgcaatcct catcatcctg ggaacccttg tgatctacac gttcgacccc tgtcccgtcg
     8641 gcgcagcgcc tgagctgctc ccgagcttct gcccggtgcc ctagtggccg accagatcgc
     8701 agacggcacc gtcatcgacg gtgttcacta ccaccagcac gagatcgtca tcgtcggcgc
     8761 gggcggtgcc gggatgcgcg cggcaatcga ggccggaccc cacgcctcca ccgcggtgat
     8821 ctccaagctc taccccaccc gctcccacac cggcgcggcg cagggcggca tggccgcagc
     8881 gctcgccaac gtggaggagg acaactggga gtggcacacc tacgacaccg tcaagggtgg
     8941 cgactacctc gtcgaccagg atgccgctga gatcctcgcg aaggaggcca tcgacgcggt
     9001 catcgacctc gagaacatgg gcctgccgt
//
LOCUS       DAOSOU010000070         8974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7740_length_8974_cov_6.162686, whole genome shotgun sequence.
ACCESSION   DAOSOU010000070 DAOSOU010000000
VERSION     DAOSOU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7740_length_8974_cov_6.162686"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..929
                     /locus_tag="PLW15_05430"
     CDS             36..929
                     /locus_tag="PLW15_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT33877.1"
                     /translation="MSRAAASVLWGVLGVLAVMVAWELYKFLGPADGFVVGAVDGETG
                     SGVMILPRTNDRAMPHLWEMGARLFQSTSGGSTPPLIVPVVTAALTTLGIAAVGWLIG
                     VAVGVILGVVMQRYRLAEWGLLPWIVVSQTVPLIAFAPLLASIGSQIDRGGFPWPQWL
                     SVAVIASYLAFFPVAIGVLRGLETPDRIHLDLMRSYASGYWSTLFRLRFPAAVPHLLP
                     ALRLAAASAVLGAVVAEVSIGMRGGIGRMLIQLAGQASSDPAAPWGPTFGSIALGLVA
                     AGSVALLGLGLKNYRRGEATA"
     gene            926..1738
                     /locus_tag="PLW15_05435"
     CDS             926..1738
                     /locus_tag="PLW15_05435"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT33878.1"
                     /translation="MSAQDQATADLAVRATGVGKVFATATGDVVALTDVELTVEAGEF
                     VSLIGPSGCGKSTLLRLIADLDDASSGQLSIFGKTARQARIDQDYGIAFQQAGLLPWR
                     TVAGNIELPLELHKVGRDARAARVAELAQLVGLSDFMDRYPDQLSGGMQQRVAIARAL
                     AEQPRLLLMDEPFGALDEMTREYLQSELARIAAETGAAVVFVTHSIPEAVFLSDRVVV
                     MSPRPGRITDILDVPFGNERTEALRESPEYFERVTAVREALHGTPVERANER"
     gene            1735..2517
                     /locus_tag="PLW15_05440"
     CDS             1735..2517
                     /locus_tag="PLW15_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT33879.1"
                     /translation="MSLAAQEREPARWVRVVAPVAVGLIMFALWVLIVDVLKIAPRAL
                     PSPFAVAGELVTHADIILEDMGITATNALIGLAAGAIVAIVAAGLAATFRPIDGMLAP
                     LVAAIAVVPIVAITPILNTMFGASSQFGRQSIAALAAFVPVFINVVRGLRQTRPVHRD
                     LLRASGASGSQTFRFLTLPTALPYLVTGLRVASSLAVIAALVAEYFGGPADGIGTAIA
                     GYAKSGKAALAFAYVAGGVAIGLAFFLVTLLLEWLVTRRRPT"
     gene            2564..3727
                     /locus_tag="PLW15_05445"
     CDS             2564..3727
                     /locus_tag="PLW15_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33880.1"
                     /translation="MRHSIRRGLTVASVATVAAIALAGCSTPAPSDTSSDFTPLTSIK
                     LQLQWLPQAQFAGYYVALDQGYFEEEGFDTVDIVPSGGDIVPQDALVAGDVDFAIAWV
                     PKVLGTLEATGAELTDIAQVYQKSGTLQVSFADKGVPTVADFEGKRIGSWGFGNEWEI
                     FAAMAAEGLDASTVSITTQDFSMNALLDGDVDAAQAMTYNEWAQILEVVNPATGKLYQ
                     PEDFDVVSYEDTDGAMLQDAIWADTARLADDPAYADAAVRFLKAVTKGWIFARDNPEE
                     AATITYDAAINAEAAFPVGPVHQLWQMNEVNKLIWTGSKFGIVDKAAWDKTVKGALSA
                     VNQDGVSLITAEPAASAYSNEYIEKALAALEAEGVDVSGAYTPIDVVLTEGGQ"
     gene            3799..5136
                     /locus_tag="PLW15_05450"
     CDS             3799..5136
                     /locus_tag="PLW15_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT33881.1"
                     /translation="MNDLNPSDGELAYELDRAHVFHSWSAQGALKPLVIASGAGCEVW
                     DFDGNKLLDFSSQLVNTNLGHQHPAVVAGIKAQADVLATVAPATANLARGEAAKRILA
                     KAGAHFGKVFFTNGGADANENAIRMARLTTGRDKVLSTYRSYHGNTGAAIVATGDWRR
                     MPNEYARGHGHFFGPFLYRSEFWADSPQQESERALTHLERVIQSEGSATIAAILLETV
                     PGTAGVLVPPPGYLEGVRRLCDKYGIVLIFDEVMCGFGRTGEWFAWQGLGEGGTAVVP
                     DLVTFAKGVNSGYVPAGGVLISESIANVFENQVFPGGLTYSGHPLAMASIVAAIDAME
                     SEGVIENAKMIGEQHLGPGLAELAAKHPLIGEVRGLGVFWALDLVNDPESREPVSGGV
                     VLELKKALMERGLLPFMADNRLHVVPPCIVTPEEVARALAIYDEAFTAVEAAL"
     assembly_gap    5153..5252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5417..5941
                     /locus_tag="PLW15_05455"
     CDS             5417..5941
                     /locus_tag="PLW15_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOT33882.1"
                     /translation="MPVFDLMHRPGEMREKSLDVVVPEGFGNAVIGVRTGSAMHIDTR
                     LESLHDGILVSGRVDATAEGECVRCLIDVALPIEVEFQELFAYSFDEAFDYTVHDDYV
                     DLEPVVRDAVVLSLPFQPVCQEDCLGLCPQCGVRLLDNPGHEHEAPVDPRWAALAGLD
                     GLNDDTESDKTEKR"
     gene            5944..6147
                     /gene="rpmF"
                     /locus_tag="PLW15_05460"
     CDS             5944..6147
                     /gene="rpmF"
                     /locus_tag="PLW15_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT33883.1"
                     /translation="MAVPKRKMSRASTRMRRAQWKADAPTLVKTVENGKTTYSLPHRA
                     KVVEDSTGTPLYMEYKGRKVADV"
     gene            6164..6862
                     /gene="rnc"
                     /locus_tag="PLW15_05465"
     CDS             6164..6862
                     /gene="rnc"
                     /locus_tag="PLW15_05465"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT33884.1"
                     /translation="MGDTVVPDDNSELSSVLGVSVAPELLQLALTHRSFAYENGGLAH
                     NERLEFLGDSILGQAVTVMLYTENPDLDEGDLAKRRASLVSSVALAEVARAIGLGKHV
                     RLGRGEEQTGGRDKSSILADTVEAIIGAAYLDRGGDEATALVLRLIRPLLDNPDRFGA
                     AMDPKTSLQELSTRLGRGMPSYEITDSGPDHSKRFQASVLLTGEVIATGVGSSKKQAE
                     MAAALDAWTLLSAK"
     gene            6869..7765
                     /gene="mutM"
                     /locus_tag="PLW15_05470"
     CDS             6869..7765
                     /gene="mutM"
                     /locus_tag="PLW15_05470"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOT33885.1"
                     /translation="MPELPEVEVVRAGLAPAVSGAGVASVTVYDERSLRRHDGPSEDF
                     IDRLTGRRMLVPERRGKFLWIPLAPVPEVLEGAGSSASPTEALVIHLGMSGQVLLRDQ
                     STDDALTRIRLDLDTGYRLNFVDQRIFGSMAIDTLVPTVDHPDQLVPSQVAHIARDLV
                     DPLFDDRRFLAALKRKNTTIKRALLDQTLVSGIGNIYADESLWGARIHYDQPTASLSR
                     PRAVALLAELRAVLAQALAEGGTSFDTQYLNVNGQSGYFAHSLKAYGQQGKPCPRCGT
                     PMVREQFMNRGSHFCPRCQRLR"
     gene            7820..>8974
                     /locus_tag="PLW15_05475"
     CDS             7820..>8974
                     /locus_tag="PLW15_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT33886.1"
                     /translation="MTSGTSNGTGAEALLGLDGLLTDDQRAWRDRARAVASVIAETID
                     DDAETQTFRREYVGLLAEAGLIGMHLSGYGCAGADAISYGLACYELEAVDTAWRTFVS
                     VQGSLAMSAIHHFGSEEQKQRWLPLMARGEALGCFALTEPDGGSDPGSMSTSARQADD
                     GTGWIIDGTKRWIGLASVADVAVVWARTPEGIRGFLVDTTDPGFSASDIPTKLALRAS
                     IQCDVVLESVRGELLPGTTGLSSALACLTEARYGIVWGVMGAARASLDEAIERATTRE
                     VFGAPIGSKQLVQQRLADAFVEYEKAMLLALHLGALKQAKTLTPEQVSVGKLNNVREA
                     IGIAHAAREILAGDGVTTEFSVMRHLANLEAVRTYEGTDDIHTLVIGRALT"
BASE COUNT         1399 a         3118 c         2921 g         1436 t
ORIGIN      
        1 atcccggcgc tccgagagca cgtcgtggcg aaggcatgag tcgcgcggcg gcttccgtgc
       61 tctggggggt gctcggtgtg ctcgccgtga tggtggcgtg ggagctgtac aagttcctcg
      121 ggccggctga cggcttcgtg gttggagcag tcgatggcga gaccggctcg ggagtgatga
      181 ttctcccgcg caccaacgac cgcgccatgc cccacctgtg ggagatgggc gcccggctgt
      241 tccagtcgac cagcggcggc agcactccgc cgctcatcgt tcctgtggtc accgcagcgc
      301 tcacgacgct cggaatcgcc gcggtcggct ggctgatcgg ggtcgcggtc ggcgtcatcc
      361 tgggtgtcgt catgcagcgt tatcgcctgg ccgagtgggg ccttctgccg tggatcgtgg
      421 tcagccagac cgtgccgctc atcgcgttcg ctccgctgct ggccagcatc ggttcacaga
      481 tcgaccgagg cggattcccc tggccgcagt ggctgtcggt ggccgtcatc gcctcctacc
      541 tcgcgttctt cccggtcgcc atcggcgtgc tgcgcgggct cgagacgccc gaccgcatcc
      601 acctcgacct catgcgcagc tacgcgagcg ggtactggtc gacgctgttc cggctgaggt
      661 tcccggccgc cgtgccgcac ctgctgcccg cgttgcggct cgccgccgca agcgccgtgc
      721 tcggggccgt cgtcgccgag gtgtccatcg gcatgcgcgg cggcatcggc cgcatgctca
      781 tccagctggc aggccaggcc tcgagcgacc cggccgcgcc gtggggaccg actttcggat
      841 cgatcgccct cggcctcgtc gccgccgggt ccgtcgccct gctgggcctc gggttgaaga
      901 actatcgcag aggggaggcg acagcatgag cgcacaggat caggccacag cagatctcgc
      961 cgtacgggcc accggagtcg gcaaggtctt cgcgaccgcc acgggggatg tcgtggcgct
     1021 gaccgacgtc gagctcactg tcgaggcggg cgagttcgtc tccctcatcg gaccgagcgg
     1081 atgcggcaag tcgaccctgc tgcgcctcat cgccgatctc gacgacgctt cgtcgggcca
     1141 gctcagcatc ttcggcaaga ccgcccgtca ggcccgcatc gatcaggact acggcatcgc
     1201 attccaacag gccggcctgc tgccctggcg caccgtcgcg ggcaacatcg agcttccgct
     1261 cgagctgcac aaggtgggcc gggatgcccg tgccgcccgt gtcgcggagc tcgcccagct
     1321 cgtggggctc tccgacttca tggaccggta tcccgatcag ctctccggcg gcatgcagca
     1381 gcgcgtcgcc atcgcccgcg cgctcgccga gcagccgcgc ctgctgctga tggacgagcc
     1441 gttcggcgcg ctggacgaga tgacgcgaga gtatctgcaa tccgagctcg cccgtatcgc
     1501 ggcggagacc ggcgccgccg tcgtgttcgt cacccactcg atccccgagg cggtgttcct
     1561 ctccgaccgc gtcgtcgtca tgagcccgcg gccgggacgc atcaccgaca tccttgacgt
     1621 gcccttcggc aacgagcgca ccgaagcact gcgcgaatcg ccggagtact tcgagcgggt
     1681 caccgccgtg cgcgaagccc tgcacggaac acccgtcgag agggcgaacg agcgatgagt
     1741 cttgctgcgc aggagcgcga accggcccgc tgggtgcggg tcgtcgcgcc cgtcgcggtc
     1801 ggcctgatca tgttcgcgct gtgggtgctc atcgtcgatg tgctgaagat cgcgccgcga
     1861 gcgctgccca gccccttcgc ggtcgcgggt gaactcgtca cgcacgctga catcatcctc
     1921 gaagacatgg gcatcaccgc gacgaacgca ctcatcggcc tcgccgccgg agcaatcgtc
     1981 gcgatcgtcg cggccgggct ggccgccaca ttccgcccca tcgacggcat gctcgccccg
     2041 ctcgtcgccg cgatcgcggt ggtgccgatc gttgcgatca cgccgatcct caacacgatg
     2101 ttcggggcgt cgagccagtt cggtcgtcag tcgatcgctg cccttgccgc gttcgtgccg
     2161 gtgttcatca acgtagtgcg cggacttcgt caaacccggc cggtgcatcg cgacctgcta
     2221 cgggcatccg gagcgtccgg gtcgcagacg ttccgctttc tcacgctgcc gacggcgctg
     2281 ccgtatctgg tcaccgggct gcgcgtcgcg agctcgctcg ccgtcatcgc cgccctggtg
     2341 gcggagtact tcggtggccc agccgatgga atcggcacag ctattgcggg gtacgcgaaa
     2401 tcgggcaaag cggccctggc gttcgcgtac gtggccggtg gcgtggccat cggactcgca
     2461 ttcttcctcg tcacgttgct gctcgagtgg ctcgtgacga ggcggagacc gacctagtcg
     2521 gaacctgcag cacccccgct ccatccctca cgaaaggaac agcatgagac acagcatcag
     2581 gcgcggcctt acggtcgctt cggtcgcaac ggtcgcggcg atcgccctcg cgggctgctc
     2641 gacacccgca ccgtcggaca cgtcgagcga tttcaccccg ctcacctcga tcaagctcca
     2701 gctccagtgg ttgccgcagg cgcagttcgc cggctattac gtggccctcg accagggcta
     2761 cttcgaggag gagggtttcg acaccgtcga catcgtcccg tcgggcggcg acatcgtgcc
     2821 gcaggacgcc ctcgttgccg gtgacgtcga cttcgcgatc gcgtgggttc cgaaggtgct
     2881 cggcaccctc gaggccaccg gcgccgagct caccgacatc gcgcaggtct accagaagtc
     2941 cggaacgttg caggtctcgt tcgccgacaa gggtgttccc accgttgccg acttcgaggg
     3001 caagcgcatc ggctcgtggg gcttcggcaa cgagtgggag atcttcgccg ccatggctgc
     3061 cgaggggctc gacgcctcca cggtctccat cacgacgcag gacttctcga tgaacgctct
     3121 gctcgatggt gacgtcgacg ccgcacaggc gatgacctac aacgagtggg cgcagatcct
     3181 cgaggtcgtc aaccccgcca ccggcaagct ctaccagccg gaggacttcg acgtggtctc
     3241 gtacgaggac accgacgggg ccatgctcca ggacgcgatc tgggccgaca ccgcccgcct
     3301 cgctgacgac cctgcctacg cggatgccgc ggtgcggttc ctcaaggccg tcaccaaggg
     3361 ctggatcttc gcgcgcgaca accccgagga ggctgcgacc atcacgtatg acgccgccat
     3421 caacgcggaa gccgcattcc cggtcggacc ggttcatcag ctctggcaga tgaacgaggt
     3481 caacaagctc atctggaccg gaagcaagtt cggtatcgtc gacaaggcgg cctgggacaa
     3541 gacggtcaag ggtgctctct ccgccgtcaa ccaggacggc gtcagcctca tcaccgcgga
     3601 gcctgccgcg tcggcgtact cgaacgagta catcgagaag gccctcgccg ctctcgaggc
     3661 cgaaggcgtg gacgtgagcg gggcgtacac cccgatcgat gtcgtcctca ccgagggtgg
     3721 ccagtaacaa ccagcactaa tccggggcgg gtggctggcc tcacgccggc cacccgcccc
     3781 accatgaaaa ggaacaccat gaatgacctg aacccgtccg acggcgagct cgcctacgag
     3841 ctggaccgcg cgcacgtctt ccactcctgg tcggcacagg gcgcactgaa gcctctcgtg
     3901 atcgcctccg gcgcgggctg cgaggtgtgg gacttcgacg ggaacaaact gctcgacttc
     3961 tccagccagc tcgtcaacac gaacctgggg caccagcatc cggctgtcgt cgcgggcatc
     4021 aaggcccagg ccgatgtgct tgccacggtc gcccccgcga ccgcgaacct tgctcgcggc
     4081 gaggccgcca agcgcatcct cgccaaggcg ggcgcgcact tcggcaaggt cttcttcacc
     4141 aacggcggtg ctgatgccaa cgagaacgcc atccggatgg cgcggctcac gaccggccgc
     4201 gacaaggtgc tctccaccta ccgcagctac cacggcaaca ccggtgcggc catcgtcgcc
     4261 accggtgact ggcgacggat gcccaacgag tacgcccgcg gacacggaca cttcttcggc
     4321 cccttcctct accgctccga gttctgggca gacagccccc agcaggagtc ggagcgcgcc
     4381 ctcacgcacc tcgagcgcgt gatccagtcg gaggggtcgg cgacgatcgc cgcgatcctg
     4441 ctcgagaccg tgcccggcac cgccggcgtg ctcgtgccgc cgcccggcta cctcgagggc
     4501 gtgcgccgcc tgtgcgacaa gtacggcatc gttctgatct tcgacgaggt catgtgcggc
     4561 ttcggtcgca cgggcgagtg gttcgcctgg cagggcctcg gcgagggcgg cacggccgtc
     4621 gtgcccgacc ttgtgacctt cgcgaagggc gtcaactccg gctacgttcc cgccggtggc
     4681 gtgctcatct cggagtcgat cgcgaacgtg ttcgagaacc aggtgttccc cggcggactc
     4741 acctactccg ggcatcccct cgccatggcc tcgatcgttg cggcgatcga cgcgatggag
     4801 tccgagggcg tcatcgagaa tgcgaagatg atcggcgagc agcacctcgg ccctggtctc
     4861 gccgagcttg cggcgaagca tccgctcatc ggggaggtgc gcggtctcgg cgtcttctgg
     4921 gcgctcgacc tcgtcaacga ccccgagtcg cgcgagcccg tctcgggtgg tgtcgtgctc
     4981 gaactcaaga aggcgctcat ggagcgcggg ctgctgccct tcatggcgga caaccgcctg
     5041 cacgtcgtgc ccccgtgcat cgtgacgccc gaggaagtcg cgcgggcgct ggcgatctac
     5101 gacgaggcgt tcacggcggt ggaagccgcg ctctgatcga ccgggccacc ggnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgatgctc ccgttgtgca ggaccagtga
     5281 gcgaatcacc ggtgtcctgc acaacaggag tgtccagcag gagtcacgcg ggtgccaagt
     5341 ggaccgacgg ccccggcgca ccgcaacgcg ggtagaatcg tccctcgtgt cccacctcat
     5401 ccagaccccg tacaccgtgc cggtgttcga cctcatgcac cgccccggcg agatgcgcga
     5461 gaagtcgctc gacgtcgtcg tgcccgaggg gttcggcaac gccgtgatcg gggtgcgcac
     5521 gggctccgca atgcacatcg acacccgttt ggagagcctc cacgacggca ttctcgtctc
     5581 cgggcgagtg gatgcgacgg ccgaagggga gtgcgttcgg tgcctgatcg acgtcgccct
     5641 gccgatcgaa gtcgaatttc aggagctttt cgcgtattct ttcgacgaag cttttgatta
     5701 cacggttcac gacgactacg tcgacttgga acccgtagtc agggatgcgg tggtcctgtc
     5761 actgccattc caaccggtct gtcaggaaga ttgcctcggc ctgtgcccgc agtgtggggt
     5821 gcggcttctg gacaaccccg gtcacgagca tgaggctccc gtcgatcccc gatgggctgc
     5881 gctcgccggg cttgacggcc tgaacgatga cactgagtct gacaagacgg agaagagata
     5941 accatggcag ttccgaagcg caagatgtcg cgggcatcca cccgcatgcg ccgcgcccag
     6001 tggaaggctg acgcccccac gctcgtcaag acggtcgaga acggcaagac cacgtacagc
     6061 ctcccgcacc gcgcgaaggt cgtcgaggac tccaccggca cgcccctgta catggagtac
     6121 aagggccgca aggtcgccga cgtctgagtt tgtggttcgc ccgatgggcg acacagtcgt
     6181 gccggatgac aactccgagc tctccagcgt gctcggggtt tccgttgcgc ccgaacttct
     6241 tcagctcgcg ctgacccacc ggtctttcgc gtacgagaac ggcgggctcg cgcacaacga
     6301 gcgactcgag ttcctcggtg actcgatcct cgggcaggca gtcaccgtca tgctctacac
     6361 cgagaacccc gacctcgacg agggcgatct ggccaagcgc cgcgccagcc tcgtctcgag
     6421 cgtcgccctg gccgaggttg cccgcgcgat cgggctcggc aagcacgtgc gactgggccg
     6481 cggcgaggag cagaccggcg gccgcgacaa gtcgtcgatc ctcgccgaca ccgtcgaggc
     6541 gatcatcggt gccgcctacc tcgatcgagg aggggacgag gcgacagcac tcgtgctgcg
     6601 cctcatccgt ccgctgctcg ataaccccga ccggttcggg gcagccatgg accccaagac
     6661 cagcctgcag gaactgtcca cccggctcgg gcggggcatg cccagctacg agatcacgga
     6721 cagcggcccg gatcattcca agcgcttcca ggcgagcgtg ctgctcacgg gcgaagtgat
     6781 cgcgaccggc gtgggctcga gcaagaagca ggccgagatg gccgccgcgc tcgacgcctg
     6841 gacgttgctc tcggcgaagt agtccaccgt gcccgagtta cccgaggtcg aggtggtgcg
     6901 cgccgggctc gcacccgcgg tgagcggggc gggcgtggca tccgtcaccg tctacgacga
     6961 acgctcgctg cgtcggcatg acggccccag cgaggacttc atcgaccgac tgactggccg
     7021 acggatgctc gttcccgagc gccggggcaa gttcctgtgg atcccgctcg cgccggtccc
     7081 tgaggttctc gaaggggccg gttcgagtgc ctcaccgacc gaagcgctcg tcatccacct
     7141 cggaatgagc ggccaggtgc tgctgcgtga ccagtcgacg gatgacgcgc tgacccgcat
     7201 ccgcctcgac ctcgacaccg gctaccgcct caacttcgtc gaccagcgca tcttcggatc
     7261 gatggccatc gacaccctgg tgccgaccgt ggatcacccc gatcagctcg tgccgtccca
     7321 ggtcgcccac atcgcccgcg acctcgtcga tcccctcttc gacgaccgcc ggttcctcgc
     7381 tgctctgaag cgcaagaaca cgaccatcaa acgcgccctg ctcgaccaga ccctggtgag
     7441 tggcatcggc aacatctacg ccgacgagtc gctctggggc gcgcgcatcc actacgacca
     7501 gcccacggcc tcgctgagcc gtccgcgcgc tgtcgccctg ctcgccgagc tgcgcgccgt
     7561 gctcgcgcag gcgctcgccg agggtggcac gagtttcgac acccagtacc tcaacgtcaa
     7621 cgggcagtcc ggctacttcg cgcattccct gaaggcctac ggccagcagg gcaagccgtg
     7681 tccgcgctgc ggcaccccga tggtgcgcga gcagttcatg aaccgcggat cgcacttctg
     7741 cccgcgctgc cagcggctgc gctgaccggg ctgcgctgac cgggctgcgc tgaccgggct
     7801 gcgcggctag gctcgcagca tgacttcagg cacgtccaac ggcaccggcg ctgaggcgct
     7861 gctcgggctc gacggcctgc tgacggacga ccagcgggcg tggcgcgatc gagcgcgagc
     7921 cgtggcatcc gtcatcgcgg agacgatcga cgacgacgcc gagacgcaga cgttccggcg
     7981 ggagtatgtg ggcctgctgg ccgaggcggg cctcatcggc atgcacctgt cgggctacgg
     8041 ctgtgctggc gccgacgcga tcagttacgg cctggcctgc tacgagctcg aggcggtcga
     8101 caccgcgtgg cgcaccttcg tgagcgtgca ggggtcgctc gcgatgtcgg cgatccacca
     8161 cttcggctcc gaggagcaga agcagcgctg gctcccgctg atggcccgcg gcgaggccct
     8221 cggatgcttc gccctcaccg aacccgacgg tggcagcgac cccggcagca tgtcgacctc
     8281 cgcacgccaa gccgacgacg gcaccggctg gatcatcgac ggcaccaagc gctggatcgg
     8341 cctcgcctcc gtcgccgacg tggcggtggt ctgggcgcgc actccggagg gcatccgcgg
     8401 attcctcgtc gacacgaccg atcccggctt cagcgccagc gacatcccca cgaagctcgc
     8461 gctgcgcgcg tccatccagt gcgacgtcgt gcttgagtcg gtgcgaggcg agctcctgcc
     8521 cggcaccacc gggctgagca gtgcgctggc ctgcctgacc gaggcgcgct acggcatcgt
     8581 ctggggcgtg atgggggcgg cacgggcatc cctcgatgaa gccatcgagc gggcgacgac
     8641 ccgcgaggtc ttcggcgctc cgatcggctc gaagcagctc gtgcagcagc gcctcgccga
     8701 cgcgttcgtc gaatacgaga aggccatgct gctcgctctg cacctcggcg cgctcaagca
     8761 ggcgaagacg ctcacgcccg agcaggtgag tgtgggcaag ctcaacaacg tgcgtgaggc
     8821 gatcggcatc gcccacgccg cgcgcgagat cctggccggc gacggcgtga cgaccgagtt
     8881 ctcggtgatg cgtcacctcg ccaacctcga ggcggtgcgc acctacgagg gaaccgacga
     8941 catccacacg ctcgtcatcg gtcgggcgct gacg
//
LOCUS       DAOSOU010000071         8925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7797_length_8925_cov_6.338444, whole genome shotgun sequence.
ACCESSION   DAOSOU010000071 DAOSOU010000000
VERSION     DAOSOU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7797_length_8925_cov_6.338444"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1707)
                     /locus_tag="PLW15_05480"
     CDS             complement(52..1707)
                     /locus_tag="PLW15_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT33887.1"
                     /translation="MRPSSGLTFGGRYQLSSRVAIGGMGEVWQATDLVIGRTVAIKIL
                     KDEYLGDPGFLERFRAEARHAALVNHEGIANVFDYGEEDGSAYLVMELVPGEALSAIL
                     ERERVLPTDKVLDIVAQTAMALQAAHAAGLVHRDIKPGNLLITPEGRVKITDFGIARI
                     ADQVPLTATGQVMGTVQYLSPEQASGHAASPTTDIYSLGIVAYEALAGQRPFTGESQV
                     AIAMAQINETPPELPVTIAEPVRNLVYSCIAKNPADRPASAAHLARAAQSLRRGDVAA
                     AAAAVPGILGTGSHAATVGPNYGQNATQATRVLPVNGALEASEPVEERRRNPWTWPLV
                     AIVSLLAVVLVGTIIALLASPPPNDNPSSPPASSSTASSPSPTPSAPNRVFLDDTLVI
                     GRTQDDVQAYIASLNFGLVLDPKQGIAAPTEEQVGLAYSVNPKGNVRKGETITVNFYT
                     EIPPPSAPKPAAVTAPAGPWLAGDDITVNWPNYSGCPSGHPLSGFNFQITNGTAASTN
                     PISSSATSLSIELGTAGTTTIAYTALCTDFESPVSDQTSVTVN"
     gene            complement(1704..3158)
                     /locus_tag="PLW15_05485"
     CDS             complement(1704..3158)
                     /locus_tag="PLW15_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT33888.1"
                     /translation="MNKELKRVSIVVLLMFLALFGSSTVIQVLTADSLRADGRNSRTL
                     YASYSAERGPILVDGQPIAESVPTDDEFKFQRTYTDGPLFAPATGYFTLNQGNTGIEG
                     SLNDYLSGTSNSQFLDQVNALLTGQNPKGAAVELTLDRDIQQAAWDALGDLQGAVIAI
                     NPKTGAILAMVSKPTFDPNALAVHDTDQVIAAYDQLLADPAGPLFNRTLAGNLNPPGS
                     TFKLVVAAAALDSGQYTADSQFPNPPQLQLPQSDSVIINSGGDDCGGAPTVSISDAIR
                     LSCNIPFAELGQAIGTRAILAQAMKFGFNSSVSVPLESTPSVYPRALDEPQTMLSAFG
                     QSSVRASPLQMAMVSMAIANGGTLMKPNLVESITAPNLTVLQSLEPSVYGQAMSRETA
                     AAITQMMVGSVSNGAASNARIDGVDVAGKTGTAENGEGDPYTLWFTGFAPANDPQVVV
                     AVVVENGAGLGQSSYGNAIAAPIAKKVLEAVLFR"
     gene            complement(3155..>4134)
                     /locus_tag="PLW15_05490"
     CDS             complement(3155..>4134)
                     /locus_tag="PLW15_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT33889.1"
                     /translation="RYIAMFSGILLLLLPMLPLVGATRNGARLWVELGPFSFQPGELG
                     KVALAIFFAGYLVSARDSLSMVGRRFLGMQFPRARDLGPILVVFAASMLVLVFQRDLG
                     TALLYFGLFLVMLYVATGRSSWIVLGLSMFIIGAVVASQTLSYVGGRVSTWLDPFNPE
                     LYDADGGSYQLVQGLFGLADGGLIGTGLGQGRPDITPLAESDYIISALGEELGMVGLF
                     AILALYLLFISRGFRIGFAGQDDFGRLLGVGLSFVIALQVFVVIGGVTRVIPLTGLTT
                     PFLAAGGSSLVANWIIAALLLRLSDSVRSQPRLVIDPLDAADNRLVVGQ"
     assembly_gap    4135..4234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4235..4728)
                     /locus_tag="PLW15_05495"
     CDS             complement(<4235..4728)
                     /locus_tag="PLW15_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HOT33890.1"
                     /translation="MPESPRSTFTSTIRLRLRVPARLRNLELVLLLFAIAIDVAAIAL
                     VQLGALGQLDLGVLALSGLLGLLLIGMHIALRVVAPDADPLIVPIITVLNGLGIAMIY
                     RIGLADEDSGWDNGGLRQMAWTAVAIAIAIGVLLVVRNHRVLQRYRYIAMFSGILLLL
                     LPMLP"
     gene            complement(4712..5956)
                     /locus_tag="PLW15_05500"
     CDS             complement(4712..5956)
                     /locus_tag="PLW15_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT33891.1"
                     /translation="MASTDRSAAVSHVGKVRSNNQDSGYAGQYLFLVADGMGGHAGGD
                     VASAIAVKRITETDRQYTSPNDAEFALQSALLAANSLLAETVFEHGELTGMGTTVSGL
                     LRVDNHVAIAHIGDSRIYLFRDGKLTQITNDHTFVQRLVDSGRITAEEAAVHPRRSVL
                     MRVLGDVDASPEIDTTVMETKAGDRWVICSDGLSSYVAEDKIATVLATVAGAKAASDR
                     LVKESLDQGAPDNVTVVLVDVDETGDSSHNPPVTVGSAASPLSFDAEAGRRPLRLPSL
                     LLHPLKATQTDDSHFEPESDEYLNALIIEDRKRARRRRTTWLVFIAVAIAAIIGAIVL
                     GYNFTQRHYFVGASEGRVAIFQGVQQDIGPIELSHVYETTTITLTNLPLYQRQQVEAT
                     INAEDLADARRIVDKLSDARVP"
     gene            complement(5960..6475)
                     /locus_tag="PLW15_05505"
     CDS             complement(5960..6475)
                     /locus_tag="PLW15_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT33892.1"
                     /translation="MSELTLLLLRLAFLAVLWGFVFSIVYALRSDLFGQKARRMPVPA
                     ASVAAPVAATLPAAAPPAPKPAVAHDPIGSVNATRLVITSGAKEGLEIDLPEEQLTIG
                     RSSESGLVIRDDYTSTHHARLLKWNEGWVIQDLDSTNGTYLDSVRVIVPTQVPLNTPV
                     KIGTTSFELRR"
     gene            complement(6472..7191)
                     /locus_tag="PLW15_05510"
     CDS             complement(6472..7191)
                     /locus_tag="PLW15_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HOT33893.1"
                     /translation="MGLLDSFEKGLERAVNGAFAKTFKSGLQPVEITSALRRELDTKA
                     AVVSRDRILVPNSFTVRLGSVDYQRMVALGPTLIDELTELVQRHAAAQHYSFAGGISI
                     ALAEDPSLSQGMIDVTSTNVKGAVAWTPVLDIGGKRYPITHSRTIIGRGSDADITVDD
                     TGISRKHVEILWDGTRAQVNDLGSTNGSLLNGTPVAKAPLAPDSVIDIGRTRIVFRVL
                     AQAAEVDQFADLRNDRPGGAR"
     gene            complement(7222..8412)
                     /locus_tag="PLW15_05515"
     CDS             complement(7222..8412)
                     /locus_tag="PLW15_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33894.1"
                     /translation="MGSTTRGVALNSTLIVVAQSTQAIAVGAIALFLPLIRSELDLSF
                     SQAGLLAAVATLSYAFMQIPAGLITDRVSPKLIFAIGILGANALAIAFALSTDFGWML
                     ATQAIGGIFRAFMFIPGMILIARHFTEARRATALGLFVAGGFSSNIVVNLAGPLLVDP
                     LGWRGVIILSSVLGLAVLVAFWFLGDDGPPREVPKSAPTRWVWSTAAWWLLGVIQFAR
                     LWIVSGFTFWLPAFLIEDRGFAIGAAGIVVAVSSAITAPANIGGGILSDRLKRPLLVI
                     AVSLGAMTLLLAVLGEVSSIPIMLAIIALLAVFIQLYFGPLFAVPARIFGQGVAGLSS
                     GFGNFCANLGGFASALLLGVLRDATGSFTVGFLALAGMALVGFAAVVALWRTGSARDH
                     RLTS"
     gene            8486..8572
                     /locus_tag="PLW15_05520"
     tRNA            8486..8572
                     /locus_tag="PLW15_05520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8520..8522,aa:Leu,seq:cag)
BASE COUNT         1512 a         2723 c         3171 g         1419 t
ORIGIN      
        1 gaacccgaca gtatctctca gagtgtaccc ggtgggttct gcgtcaccgt gtcagttgac
       61 tgtgactgac gtctggtcgg acacggggga ctcgaagtcg gtgcagaggg ccgtgtaagc
      121 gatagtcgtc gttcccgcgg ttcccagctc gatcgacagg ctggtcgccg acgatgagat
      181 cgggttggtg ctcgcagccg tgccgttggt gatctggaag ttgaatccgc tgagcgggtg
      241 tccgctcggg caaccgctgt agttcggcca gttcacggtg atgtcgtcgc cggcgagcca
      301 tggtccggca ggcgcggtga ccgcggccgg cttaggggcc gagggcggcg gaatctccgt
      361 gtagaagttg accgtgatcg tctcgccctt gcgcacgttg cccttggggt tgacgctgta
      421 ggccagaccg acctgctcct cggtgggggc agcgatgccc tgcttggggt cgagcacgag
      481 gccgaagttg aggctcgcaa tgtaggcctg cacgtcgtcc tgagtgcgcc cgatcaccag
      541 ggtgtcgtcg aggaacaccc ggttgggggc tgacggcgtt gggcttggtg acgacgcggt
      601 cgaggagctc gcgggcgggc tcgacgggtt gtcgttcggc ggagggcttg cgagcagcgc
      661 gatgatcgtg ccgacgagca ccacggcaag cagcgagacg atggccacga gcggccaggt
      721 ccacgggttg cgccgacgct cctcgacggg ctcggacgcc tcgagtgcgc cgttgaccgg
      781 gagcacccgc gttgcctggg tggcgttctg tccgtagttc gggccgacgg ttgcggcgtg
      841 cgagccggtg ccgaggatgc cgggcaccgc cgctgcggcg gcagcgacat ccccccggcg
      901 aagcgactgc gctgctcggg ccaggtgtgc ggccgaagcg ggtcggtcgg cggggttctt
      961 ggcgatgcag gagtagacca gattgcgcac cggctcggcg atggtgaccg gcagctcggg
     1021 gggcgtctcg ttgatctgcg ccatcgcgat ggccacctgc gactcgcccg tgaacggacg
     1081 ctgtcccgcg agagcctcgt aggcgacgat gcccagcgag tagatgtcag tggtggggga
     1141 tgcggcgtgc ccgctcgcct gctccggcga gaggtactgc acggttccca tgacctggcc
     1201 ggtcgccgtc agcggcacct ggtcggcgat gcgtgcgata ccgaagtcgg tgatcttcac
     1261 ccgaccctcg ggggtgatga gaaggttgcc gggcttgatg tcgcggtgga cgagcccggc
     1321 cgcgtgggct gcctgcaggg ccatggccgt ctgggcgacg atgtcgagga ccttgtcggt
     1381 gggcagaacc cgttcgcgct cgaggatcgc gctcagcgcc tcgcccggca cgagctccat
     1441 gacgaggtag gcgctgccat cctcctcgcc gtagtcgaag acgttggcga tgccttcgtg
     1501 attgaccagc gcggcatgcc gggcctcggc gcggaagcgc tcgaggaagc cggggtcgcc
     1561 gaggtactcg tccttgagga tcttgatggc gacggtgcgc ccgatcacga ggtcggtagc
     1621 ctgccagacc tcgcccattc ctccgatcgc gacgcgactg gagagttggt atctgccacc
     1681 gaaggtgagc ccgctgctgg gtctcatctg aatagcaccg cctctagtac tttcttcgca
     1741 atgggagctg cgatcgcgtt cccgtagctg gactgtccga gtccggcgcc gttctcgacg
     1801 accactgcaa cgactacctg tggatcgttt gccggggcaa acccggtgaa ccagagggta
     1861 tagggatcac cctcgccgtt ctcggctgta ccggtcttac ccgcgacatc gactccgtct
     1921 attctggcat tactcgccgc gccgttgctg actgagccga ccatcatctg ggttatcgcg
     1981 gcagcagtct cgcggctcat cgcctgcccg tagacgctcg gctcaagtga ctgcagcacc
     2041 gtcaggttgg gtgcggtgat cgactccacg agattcggct tcatcagagt gccgccgttg
     2101 gcgatcgcca tcgagaccat ggccatctgc agcggagacg cccgcacgct ggactggccg
     2161 aaggcggaga gcatcgtctg gggctcgtcg agtgctcgcg ggtacacgct gggagtcgac
     2221 tcgaggggaa cagacacgct cgagttgaag ccgaacttca tggcctgagc gaggatcgcg
     2281 cgcgtgccga tcgcctggcc gagttcggcg aaggggatgt tgcagctgag ccggatcgcg
     2341 tccgagatcg acacggttgg cgcgccaccg cagtcatccc cgccggagtt gatgatcacg
     2401 ctgtcgctct gcggcagctg cagctggggc ggattgggga attggctgtc ggccgtgtac
     2461 tggccgctgt cgagtgccgc ggctgcgacc acgagcttga aggtcgagcc cgggggattg
     2521 aggttaccgg ccagcgttcg gttgaacagc gggcctgccg gatcggcgag cagctggtcg
     2581 taggccgcga tcacctggtc ggtgtcgtgt accgcgagcg cgttggggtc gaacgtcggc
     2641 ttggagacca tcgcgagaat ggcgcccgtc ttggggttga tggcgatgac cgcgccctga
     2701 agatcgccga gcgcatccca ggccgcctgc tggatgtcgc gatccagggt cagctcaacc
     2761 gccgccccct tggggttctg tccggtgagc agcgcgttga cctgatcgag gaactgactg
     2821 ttggaggttc cactgaggta gtcgttgagg ctgccctcga tgccggtgtt gccctggttc
     2881 agcgtgaagt acccggtggc gggggcgaac agcgggccat cggtgtaggt gcgctggaac
     2941 ttgaattcgt cgtcggtcgg aaccgactcg gcgatcggct gcccgtcgac gaggatcggg
     3001 ccgcgctcgg ccgagtagct ggcatacagg gtgcgcgagt tgcgcccgtc ggcgcgcaga
     3061 ctgtcggcag tgagcacctg gatgaccgta ctcgagccga acagcgcgag gaacatcagc
     3121 agcaccacga tgctgacgcg cttgagttcc ttgttcactg gcccacgacc agacggttgt
     3181 ccgcggcatc cagcgggtcg atgaccagcc ggggctggct gcgcacgctg tcagacagtc
     3241 gcagcagaag cgcggcgatg atccagttcg cgaccaggga ggagccgccg gctgcgagga
     3301 acggggtggt gagtccggtc agcgggatga cgcgggtgac gccaccgatc acgacgaaga
     3361 cctgcagggc gatgacgaac gagaggccca caccaaggag gcgcccgaag tcgtcctgac
     3421 cggcgaaacc gatgcggaag ccgcgggaga tgaagagcag gtagagcgcg aggatggcga
     3481 acagccccac catgcccagc tcctcgccga gcgcggagat gatgtagtcg ctctcggcga
     3541 gcggcgtgat gtcgggccgg ccctgaccga gcccggtgcc gatcaggccg ccatccgcca
     3601 gaccgaacag cccctgcacg agctggtagc tgccgccgtc agcatcgtag agctcggggt
     3661 tgaagggatc gagccacgtg ctgacgcgtc cacccacgta actcagggtc tggctggcca
     3721 cgaccgcgcc gatgatgaac atgctcagac cgagcacgat ccaactggac cggccggtgg
     3781 cgacgtagag catcacgagg aagagtccga agtagagcag ggccgttccg aggtcgcgct
     3841 ggaagaccag tacgagcatc gaggcggcga agaccacgag gatcggaccg agatcacggg
     3901 cccgcgggaa ctgcatgccg aggaagcgcc gacccaccat ggagagcgag tcccgtgcag
     3961 agaccaggta gccagcgaag aagatcgcca gcgcgacctt gcccagctcg ccgggctgga
     4021 acgagaaggg accgagctcg acccagagcc tcgcgccgtt ccgagtcgcg cccaccagag
     4081 gaagcatggg aagcagcagg agcaggattc cgctgaacat cgcgatgtac cggtnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggaagc atgggaagca gcaggagcag
     4261 gattccgctg aacatcgcga tgtaccggta gcgctgcagc acccggtggt tgcgcacgac
     4321 gagcagcacc ccgatcgcga tcgcgatggc cacggccgtc caggccatct gccgcaggcc
     4381 gccgttgtcc cacccggagt cctcgtcggc gaggccgatg cggtagatca tcgcgatgcc
     4441 gaggccgttg agcacggtga tgatcggcac gatcagggga tcggcgtcgg gagcgacgac
     4501 ccgcagggcg atgtgcatcc cgatgagaag caggccgagc agtcccgaga gggcgagcac
     4561 accgaggtcg agctgcccca gggcgccgag ctgcacgagg gcgatcgccg ccacatcgat
     4621 ggcgatcgcg aagagcagga gaaccaattc gaggttgcgc agccgagccg gcacacgcag
     4681 tctgagccgg atcgttgagg tgaacgtcga cctaggggac tcgggcatcc gacaacttat
     4741 ccactattcg gcgggcgtcg gcgaggtcct ccgcgttgat cgtggcctcc acctgctgac
     4801 gctggtacag gggcaggttg gtgagggtga tggtggtcgt ctcgtagacg tgggagagct
     4861 cgatcggccc gatgtcctgt tgcacgccct ggaagatcgc gacccggccc tcgctcgcac
     4921 cgacgaagta gtggcgctgg gtgaagttgt agccgagcac gatcgccccg atgatggctg
     4981 cgatggccac cgcgatgaag acgagccagg tggtgcgtcg acgtcgggcg cgcttgcggt
     5041 cctcgatgat gagggcgttc aggtactcgt ccgactcggg ttcgaagtgg ctgtcgtcgg
     5101 tctgcgtcgc cttgagcgga tgcagcagca ggctcggcag gcgcagcgga cgccgaccgg
     5161 cctcggcgtc gaacgacagc ggggaggcgg cggagccgac ggtcaccggc gggttgtgcg
     5221 acgagtcgcc cgtctcatcg acgtcgacga ggacgacggt gacgttgtcg ggcgcgccct
     5281 ggtcgagact ctccttgacc aggcggtccg aggcggcctt ggcgccggcg accgtcgcaa
     5341 gcaccgtggc gatcttgtcc tcggcgacgt agctgctcag gccgtcggag cagatgaccc
     5401 agcgatcgcc ggccttggtc tccatgacgg tcgtatcgat ctcgggcgac gcatcgacgt
     5461 cgccgagcac ccgcatgaga accgagcgcc ggggatgcac ggccgcttct tctgctgtga
     5521 tgcggccgct gtcgaccagc cgctgcacga aggtgtggtc gttggtgatc tgggtgagtt
     5581 tgccgtcacg gaacaggtag atgcgggagt cgccgatgtg cgcgatcgcc acgtggttgt
     5641 cgacgcggag caggccgctc acggtcgtac ccatgccggt gagttcgccg tgctcgaaga
     5701 ccgtctcggc gagcagggag ttcgcggcaa gaagcgccga ctgcagcgcg aactcggcat
     5761 cgttgggtga ggtgtactgg cgatcggtct cggtgatgcg cttgaccgcg atcgccgaag
     5821 cgacgtcgcc gcccgcgtgg ccacccatgc cgtcggcgac gaggaacagg tactgtcccg
     5881 cgtagccgct gtcctggttg ttggagcgca ccttgccgac gtgcgagacg gccgcgctcc
     5941 tgtcagtact ggccatcacc taccgccgaa gttcgaagct ggtcgtaccg atcttcaccg
     6001 gtgtgttcag cggtacctgc gtgggaacga tcacgcggac cgagtccagg tacgtgccgt
     6061 tggtcgagtc taggtcctgg atgacccagc cctcgttcca cttcaggagc cgagcatgat
     6121 gcgtcgacgt gtagtcatcg cggatgacga gtcccgattc gctcgagcga ccgatggtga
     6181 gctgctcctc cggaaggtcg atctcgagac cctccttcgc gcccgacgtg atcaccagcc
     6241 gagtggcgtt gacggagccg atcgggtcgt gcgcgacagc gggtttcggt gccggtgggg
     6301 cggctgcggg cagggtggcc gcgaccggtg cggcgacgct ggcggcgggg acgggcatcc
     6361 gtctcgcctt ctgaccgaag aggtcggagc gcagggcgta gacgatagag aagacgaagc
     6421 cccacagcac ggcgaggaat gccagccgca gcaggagcag tgtcagttca ctcaccgtgc
     6481 accacccgga cggtcgttgc gcaggtcggc gaactggtcg acctcggcgg cctgggcgag
     6541 cacgcggaac acgatgcggg tgcggccgat gtcgatcacc gagtcggggg caagcggggc
     6601 cttggccacc ggggtgccgt tgagcagcga gccgttggtc gaacccaggt cgttgacctg
     6661 tgcgcgggtg ccgtcccaga ggatctcgac gtgcttgcgc gagatgccgg tgtcatccac
     6721 ggtgatgtcg gcatcgctgc cgcgcccgat gatcgtgcgg gagtgggtga tcgggtaccg
     6781 cttgccaccg atgtcgagca ccggggtcca cgcgacggca cccttcacgt tggtcgatgt
     6841 gacgtcgatc attccctggc tgagcgaagg gtcttcggcg agagcgatcg agatgccgcc
     6901 cgcgaatgag tagtgctggg cggcggcatg acgctgcacc agctcggtca gctcatcgat
     6961 gagcgtcgga ccgagggcga ccatgcgctg gtagtcgacc gagccgagcc gcacggtgaa
     7021 ggagttcggc accaggatgc ggtcgcgcga gacgaccgcc gccttcgtgt ccaactcgcg
     7081 acgcagggca ctggtgatct cgacgggctg cagaccactc ttgaaggtct tggcgaaagc
     7141 gccgttgacg gcgcgctcaa gacccttctc gaagctgtcc agaagaccca agactctcct
     7201 cgtgattgcc cgtactggtt atcacgatgt tagccggtgg tcccgggcgg aacctgtccg
     7261 ccacagggcg accactgccg cgaaacccac gagtgccatt cccgccagcg cgaggaagcc
     7321 cacggtgaac gaccccgtcg catcccgaag cacgccgagc agcagcgccg aggcgaaccc
     7381 gccgaggttc gcgcagaagt tgccgaagcc cgaactgagc cccgccaccc cctgaccgaa
     7441 gatgcgcgcg ggcaccgcga agagcggccc gaagtagagc tggatgaaca ccgcgagcag
     7501 ggcgatgatc gccagcatga tcgggatgct gctgacctcg ccgagcacgg cgagcagaag
     7561 cgtcatcgcc cccaacgaca cggcgatcac gagcagcggc ctcttcagcc ggtcggacag
     7621 gatgccacca ccgatgttgg ccggcgccgt gatcgccgac gacactgcga ctacgatgcc
     7681 cgcggcgccg atcgcgaatc cgcggtcctc gatcaggaat gcgggcagcc agaaggtgaa
     7741 gcccgagacg atccacagtc gggcgaactg gatgacaccc agcagccacc acgccgcggt
     7801 ggaccacacc caccgcgtcg gcgcggactt cggcacctcg cggggcgggc cgtcgtcgcc
     7861 gaggaaccag aacgccacga gcacggcgag ccccagcacc gacgacagga tgatcacccc
     7921 gcgccatccg agcgggtcga cgagcagagg ccccgcgaga ttcaccacga tgttcgacga
     7981 gaacccgcca gccacgaaga gtccgagagc ggtggcgcgc cgcgcctcgg tgaagtgtcg
     8041 tgcgatcagg atcattccgg ggatgaacat gaacgcgcgg aagatgccgc cgatggcctg
     8101 agtggcgagc atccagccga agtcggtgct gagcgcgaag gcgatggcga gagcgttggc
     8161 gccgaggatg ccgatggcga agatgagctt cgggctcacg cgatcggtga tgaggcccgc
     8221 gggtatctgc atgaacgcgt acgacagggt ggcgacggcg gcgagcaggc cggcctgcga
     8281 gaagctgagg tcgagttccg accggatcag cggcaggaac aaggcgattg cgccgaccgc
     8341 aatcgcctgc gtggactgtg cgaccacgat gagcgtgctg ttgagcgcga caccgcgcgt
     8401 ggtcgaaccc atgggactcc cgatgctagc gggcgttgct ctgtgataat ctcgctcagt
     8461 tgacgcgtga agcgtcggca ggtgagcgcg agtggcgaaa ttggcagacg cgcacggttc
     8521 aggtccgtgt gcccgtgagg gcatgggggt tcaagtcccc cctcgcgcac catgaagaag
     8581 gcgtcacccc cgggtggcgc cttcttcatt tccgggaggg gttaccgtga acccgccggg
     8641 gtccccgccg cgccggttcc ggcgcatcgc ccagtgtgag ttcgccggtt gagtagccgc
     8701 gcagcggcgt atcgaaaccg cagctacctg gcgatgtgac ggccgtgccg gtggggcgtc
     8761 aagtccccct cgtgcacagc gcgaactcag tgcgaggcgc gaacccgcgt gagcaggggc
     8821 cggacagtgc gccgcactcg cgagcgcgct cgcgtgacca agcttcggcg aggcttgggc
     8881 ggaaccttct cagtccacgc ggcggtgagt tcgaccagcc agcgg
//
LOCUS       DAOSOU010000072         8919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7807_length_8919_cov_6.412681, whole genome shotgun sequence.
ACCESSION   DAOSOU010000072 DAOSOU010000000
VERSION     DAOSOU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7807_length_8919_cov_6.412681"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLW15_05525"
     CDS             complement(<1..229)
                     /locus_tag="PLW15_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOT33895.1"
                     /translation="MDWQTPEDTVPSAFTTEANARSVLGKPPATGAWREGDPVGQRQF
                     ASIGPMTLERGGELPSVRVAYETYGTLNTDKS"
     gene            complement(342..1664)
                     /locus_tag="PLW15_05530"
                     /pseudo
     CDS             complement(342..1664)
                     /locus_tag="PLW15_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
     gene            1816..2376
                     /locus_tag="PLW15_05535"
     CDS             1816..2376
                     /locus_tag="PLW15_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT33896.1"
                     /translation="MSSIRIFILGALDARGEMHGHQLRLLAEEEHIDQWTDITVGALY
                     GAIKRLATEGLIEERRVEREGSFPERQVWGITAEGREALSVLRHRGLREIVHKPDPFD
                     LAMTRLDPNKLDDVEAILLSRLSSLRSELLDSQTHLQTIKQYLTVAEQIVMNHKAHRL
                     AADIAWHEELLGQLPEIIADELSRKD"
     gene            2378..3829
                     /locus_tag="PLW15_05540"
     CDS             2378..3829
                     /locus_tag="PLW15_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33897.1"
                     /translation="MSSAPAPAAAPPVARGAWLALAVVLGAAFMSLLDTTIVNVALPT
                     IRTSLDASESTLSWIISGYALAFGLALIPAGRLGDRYGHKWVFITGLALFTAASLACG
                     LAQNDLQLIIARVVQGLGGGIFFPAVSAFIQLLFPPMARGKAFAILGAVIGVSSALGP
                     LVGGLIIQALGDETGWRWIFAVNLPIGVIVLVAAIALLPSEAAGKATSNDLIGLVLLS
                     GALVALLVPLIQGQEEGWPLWTYLSIGGGVLLVVLFALWERNVASRGKDPLVPPRLFA
                     HAQFAAGTILALVFFAAFTSIFFTISLLWQAGLGHTALESGLVSVPFAIGNIIGASQS
                     DRLAARLGRAVLAIGVALLTIGLTALFLILLLVPTADLTNWTLLGPLLVAGLGSGLFI
                     APNVRFIVATVDRSEAGAASGVIGTMQRVGAAAGIAVIGSVFFGNIGPIAGPADLPAA
                     FGQAATPALAVSAALALVAFLLVFTLPKRVQLH"
     gene            complement(3855..4937)
                     /locus_tag="PLW15_05545"
     CDS             complement(3855..4937)
                     /locus_tag="PLW15_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT33898.1"
                     /translation="MTDSTPSPAVATPAAPKPRVPFWDNARFVCVTLVVIGHGIQRLT
                     ADSDPALSLYLFIFAFHMPAFAIISGYFSKSDPPNRVQMTKVITDILLPYLIMEAIWS
                     VVKFVLYGSVDFNPTTASWTLWFLLALAIFRLVLPYLALLRWPLAWAVLFSVAVGYWA
                     NVDSTFSLARAFGILPFFVLGWKLKQWGVMDRWLAMDRGSTWVRLGATIVLGGWLVVA
                     FVFVNQFRAINLTHWFFYDSSYADLDAAQWWGGFARLGFLVMATVLSAALFALIPRRE
                     TWITDFGQATMYVYLLHTFVLYPVRESGILGGENSSLLWLIGMVLASIALSVLLSTKL
                     VRRIFRPIIEPKPRWLFVQADGRPAA"
     gene            4971..>5200
                     /locus_tag="PLW15_05550"
     CDS             4971..>5200
                     /locus_tag="PLW15_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT33899.1"
                     /translation="MASKRAVVTGASSGIGAATVRLLRSHGWDVVGVARRADRLEALA
                     AETGADVFVADVTKQADVDALRDHLAALGPIHS"
     assembly_gap    5201..5300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5301..5471
                     /locus_tag="PLW15_05555"
     CDS             <5301..5471
                     /locus_tag="PLW15_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOT33900.1"
                     /translation="DAVYDNVPNPLYAEDVAEAIVHSIELPFHVDLDLVTIKPVAQAA
                     PHKVVRGELKPR"
     gene            complement(5479..6180)
                     /locus_tag="PLW15_05560"
     CDS             complement(5479..6180)
                     /locus_tag="PLW15_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOT33901.1"
                     /translation="MPDIAARFAELTTPHLADACVRAGVEVRCAPAQIQSLSAGMRLI
                     GRVLPARHVGSVDVFLEAIENAQPGDVLVADNRGRVDESCIGDLMAQEAASAGLAGIV
                     IWGLHRDTVDIAAIGIPLYSCGSIPTGPLSVDARPSAALDSAQIGDWTVDFSDLVAAD
                     DDGVLFLPADRIDELLTLAEGIRDTERRQADRIVDGVTLRQQVRFSDYLAARAANPAL
                     TFREHLRTVGGEIEV"
     gene            complement(6180..7058)
                     /locus_tag="PLW15_05565"
     CDS             complement(6180..7058)
                     /locus_tag="PLW15_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HOT33902.1"
                     /translation="MDEVQVGVVRRFNRTVTQRVGALNDSFLSRGRPLGHSRVLWEIG
                     DGAELRELRARLDLDSGYLSRILRSLETDGLATVSTDGDDRRVRRVALTPAGLRERAA
                     LDELSDAQASAMLDPLGDRQRVELAGAMATVSRLLEASAITVAIADPASPAVRTSMAA
                     YFDELARRFEAGFDPAVTLPTADEQFRLPAGLVLLATLHDRPVGCVGLKLHADGVGEV
                     KRLWVSSEVRGLGLARRMLGRIEAEGSSRGIHTLRLDTNRALTEAIALYRALGYREIE
                     AYNDEPYAHHWFEKRI"
     gene            complement(7113..8600)
                     /locus_tag="PLW15_05570"
     CDS             complement(7113..8600)
                     /locus_tag="PLW15_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT33903.1"
                     /translation="MSLVKEKAGAITFGLVGFLFLVELVSGILQGFYVPLIPDLVDHL
                     GIKDADFNWFEAAQLLLSAIVVPILAKCGDMFGHKRILLIATVLTAGSTWALAFTNSF
                     TTFLIAWALQGFYVVWLPLEVALIFDRGRVTGRGASQTRRAAGYLVVALEAGAIIGAL
                     GAGRIFKALGGDVQTTLMVPAVAVTLVFFAILFGVPESKPLPGRSLDLWGFVLLTIGL
                     LFITSGLTFLRINGPTAWWCYLLMVVGVLIFIPWSRYELKQKDPAIDLRVLKQPNMWP
                     VQLTAGLVGISLLGAQAPLATFAGTDPINGYGLGMAADEISYIIGAYLVSMIVGALLF
                     PRVSKWFSPRIALIIATFFVATGYLLFLVNHTSVAGVFVNMAIAGIGSGALVGALPSA
                     AAAAAPRGQTGIATALTNTTKTIGGSFASATFAIVLAAGAAGAVTATASSLLGYLTVF
                     TICGVGALVAAVLLFLVPKLAFADVEEALIEEATLAAEGTTRVAD"
     gene            8672..>8919
                     /locus_tag="PLW15_05575"
     CDS             8672..>8919
                     /locus_tag="PLW15_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOT33904.1"
                     /translation="MADDEREVLTWSDFGSASRDLALDVIRSGFEAEVVVAIARGGLL
                     LAGGIAYALGLKSCGAVNAEFYTGVGTRLDTPILLPPFL"
BASE COUNT         1447 a         2886 c         3056 g         1430 t
ORIGIN      
        1 tcgacttgtc ggtgttgagc gttccgtagg tctcgtaggc cacccggacc gacggcagct
       61 cgccgccgcg ttcgagtgtc atcggcccga tggaggcgaa ctgtcgctgc ccgaccgggt
      121 cgccttcgcg ccacgcgccg gtggcaggag gcttgccgag cactgagcgc gcgttcgcct
      181 cggtcgtgaa ggccgaggga acggtgtcct cgggagtctg ccagtccatg tcgagtcaag
      241 tatcccggaa acggcgaagc gcccccgcct ttgttacagc gggggcgctt cggtggccga
      301 gcttgtcgag accgatctcg acttcgctcg atcaccgcac gttacttggc tgcgttggcc
      361 gccgcgatcg tgcgagcggc cgcgaagccc gcttcgaggt cggccttgag gtctgccacg
      421 ttctcgatgc cgaccgagag ccgcacgagg cccggcgtga cgccggccgt gagctgctgc
      481 tccgggctca gctgcgagtg ggtggtggag gcggggtgga tgatcaggct gcgcacatcg
      541 ccgatgttgg cgaggtggct gaacagctcg acgttctcga cgagggcgcg acccgcgtcg
      601 actccgccct tgagctcgaa ggagacgacg gcaccgacgc ccttgggggc gtacttgttg
      661 gcggcggcgt accacgggct ggagggcagg cccgagtagt tgactgacgc gacatccggg
      721 tggttctcga gccactcggc gatctcctgg gtgttctgca cgtgacgctc gatgcgcagg
      781 ctcagggtct cgattccctg gatgatcgag aaggcgctgt cgggggacag tgcggcaccg
      841 gtgtcgcgca gcagctgaac gcggatcttg atcacgtagg cgatcgcatc cccgagcacg
      901 gtggtgtagc tcgcgccgtg gtagctgggg tcgggctcgg tgaggccggg gaacttctcg
      961 gcgttcttcg cccacgggaa cttgccgccg tcgacgatga cgcccgcgat gaccgttccg
     1021 tgaccaccga ggaacttggt ggccgaatgc acgacgatgt cggcgccgaa ctcgaacggg
     1081 cggatcaggt agggagtcgc gatcgtgttg tcgacgatga ggggcaggtt gttgctgtgg
     1141 gcgacgtcgg cgacgcgctc gatgtcgagg acgttgatct tggggttacc gatcgtctcc
     1201 gcgaagaaca gcttggtgtt cgggcgcacg gccgcggccc actcggcctc gtcgtcctgg
     1261 ttctcgacga aggtgacctc gatgccgagc ttggccagcg tgtacttgaa caggttgtag
     1321 gtaccgccgt agatcgacga cgacgacacg atgtggtcgc cggcgccggc gatgttgagc
     1381 accgcgtagg tgatcgccga tgagccggat gccacggcga gcgccgcggt tccgccctcg
     1441 agcgcggcga cccgctgctc gagcacgtcg gtggtcgggt tcatgaggcg ggtgtagatg
     1501 ttgccgaact ccgccagcgc gaagaggttc tgggcgtgcg ccgtgtcgtt gaacacgtac
     1561 gaggtggtct tgtagatcgg ggtggcgcga gcgttggtgg tcgggtcggg cttggcgccg
     1621 gagtggacct gcttggtttc gaacttccag tcggacatgg cggactcctt ggttgggctg
     1681 agtgcggaaa gacggtgacg ggcgggactt cacgcccact caagagtgag cataggaagc
     1741 cgctgggtgg gcggcaatcg cctgcgaaac atgacgtaac ttgacacgcg cgactactct
     1801 tgcgagagta ctgccgtgtc atccatccga atcttcatcc tcggcgcact ggacgcccgg
     1861 ggggagatgc acggtcatca gctccggctc ctggccgagg aggagcacat cgaccaatgg
     1921 accgacatca ctgtgggagc tctctacggg gcgatcaagc gcctcgcgac cgagggcctc
     1981 atcgaggaac gccgggtcga gcgcgaggga tccttccccg aacgtcaggt gtggggcatc
     2041 acggccgagg gccgcgaggc gctcagcgtc ctgcgtcatc ggggtcttcg cgagatcgtg
     2101 cacaaacccg atcctttcga cttggccatg acccgactgg atcccaacaa gctcgacgac
     2161 gtcgaggcga ttctgctcag tcgcctgtcg tcgctgcgat cagaactgct cgactcccag
     2221 acccacctcc agacgatcaa gcagtacctc accgttgccg agcagatcgt catgaatcac
     2281 aaggcgcatc ggcttgcggc cgacatcgcc tggcacgagg aactgctcgg ccagctgccc
     2341 gagatcatcg ccgacgaact gtccagaaag gactgacatg tcatccgccc ccgcccccgc
     2401 agcggcgccc ccggtcgccc gcggagcctg gcttgctctc gccgtcgtgc tcggggccgc
     2461 attcatgtcc ctgctcgaca ccacgatcgt gaacgtcgcg ttgccgacaa tccgcaccag
     2521 tttggatgcc tcggagtcga ccctctcgtg gatcatctcc ggctacgccc tcgccttcgg
     2581 gctcgcgctc atccccgcgg gccgcctggg ggaccgctac ggccacaagt gggtgttcat
     2641 caccggcctc gccctgttca ccgcggcaag cctcgcgtgc gggctcgccc agaacgatct
     2701 gcagctcatc atcgcgcgcg tcgtgcaggg tctcggcggc ggcatcttct tccctgcggt
     2761 gagcgcattc atccagctgc tcttcccgcc gatggctcgc ggcaaggcgt tcgccatcct
     2821 cggtgcggtc atcggtgtct cgtcggcgct cggtcccttg gtgggcggtc tgatcatcca
     2881 ggccctcggt gatgagaccg gctggcgctg gatctttgcg gtcaacctgc cgatcggcgt
     2941 catcgtgctg gtcgcggcca tcgcgctgct tccctccgaa gccgcgggca aggcgacctc
     3001 caacgatctc atcggcctcg tgctgctgtc gggcgcgctc gtcgcgctcc tggtcccgtt
     3061 gattcagggc caggaggagg gctggccgtt gtggacctac ctgtcgatcg gcggcggtgt
     3121 gctgctcgtg gtgctgttcg cgctctggga gcggaacgtg gcatcccgtg gaaaggaccc
     3181 gctggtgccg ccgcgcttgt tcgctcacgc gcagttcgcc gcgggcacga ttctcgccct
     3241 ggtgttcttc gccgccttca cgagcatctt cttcacgatc tcgctgctct ggcaggccgg
     3301 actcggccac accgcactgg agtcgggcct cgtgagcgtg cccttcgcga tcggcaacat
     3361 catcggcgcg agccagagtg accgtctcgc ggcacgactc ggccgggcag tgctggccat
     3421 cggggtggca ctgctcacga tcggcctcac cgcgctgttc ctgatcctgc tgctggttcc
     3481 caccgccgat ctgaccaact ggacactgct cggtcccctc ttggtcgcag gcctcggcag
     3541 cggcctgttc atcgccccca acgtgcgctt catcgtggcg acagtcgacc ggtccgaggc
     3601 gggcgccgcg agcggagtga tcggcacgat gcagcgcgtc ggcgcggctg cgggcatcgc
     3661 cgtgatcggc agcgtgttct tcggcaacat cgggccgatc gccggtcccg ctgacctacc
     3721 cgcggcgttc ggtcaggccg cgaccccggc gctcgccgtg agtgcggcgc tcgcgctggt
     3781 cgcgttcctg ctggtcttca ccctgcccaa gcgggtgcag ctgcactgag catgttctgc
     3841 agcgagcccg tcgttcaggc ggccggtcga ccgtccgcct gaacgaacag ccacctcggc
     3901 ttgggctcga tgatcgggcg gaagatgcgg cgcacgagct tcgtcgacag cagcaccgac
     3961 agtgcgatgc tcgcgagcac catgccgatg agccacagga gcgagctgtt ctcgccgccg
     4021 aggatgcccg actcgcgcac ggggtagagc acgaatgtgt gcagcaggta gacgtacatc
     4081 gtcgcctggc cgaagtcggt gatccaggtc tcccgacgcg ggatcagagc gaacagtgcc
     4141 gcgctcagca cggtggccat caccaggaat ccgagccgcg cgaatccgcc ccaccactgg
     4201 gcggcatcga ggtcggcgta cgacgagtcg tagaagaacc agtgggtgag gttgatggcc
     4261 ctgaactggt tgacgaagac gaaggcgacg acgagccacc ccccgagcac gatcgtggcg
     4321 ccgagtcgca cccaggtgct tccgcgatcc atggcgagcc aacggtccat cacgccccac
     4381 tgcttgagct tccagccgag cacgaagaac ggcaggatgc cgaacgcgcg cgccagcgag
     4441 aaggtgctgt cgacgttggc ccagtacccg accgccaccg agaacagcac ggcccaggcc
     4501 agcggccagc gcagcagggc gaggtagggc agcacgagcc ggaagatcgc cagcgcgagc
     4561 aggaaccaca gggtccagct cgccgtggtg gggttgaagt cgacggagcc gtagagcacg
     4621 aacttcacca ccgaccagat cgcctccatg atcaggtacg gcagcaggat gtcggtgatg
     4681 accttcgtca tctgcacccg gttcggcgga tcggacttcg agaagtagcc ggagatgatc
     4741 gcgaacgcgg gcatgtggaa cgcgaagatg aacaggtaga gggacagcgc ggggtctgag
     4801 tcggcggtga gccgctggat gccgtggccg atcacgacga gcgtgacgca gacgaagcgc
     4861 gcgttgtccc agaagggaac gcgcggcttg ggtgcggcgg gcgtggccac cgcgggggag
     4921 ggcgtgctgt cggtcatcgg caccacccta cccgcgctac tttggtgggt atggcatcca
     4981 agcgcgcagt ggtcaccggg gcgagttcgg gcatcggggc ggcgacggtg cgcctgctgc
     5041 gctcgcacgg ctgggatgtc gtcggcgtcg cgcggcgcgc cgaccgcctg gaggctctcg
     5101 cggccgagac cggtgccgac gtcttcgtcg ccgacgtcac gaagcaagcc gacgtggatg
     5161 ccctgcgcga ccacctcgcg gcgctgggcc cgatccactc nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn gacgccgtct acgacaacgt gcccaacccg ctgtacgccg
     5341 aggacgtggc cgaggcgatc gtgcactcga tcgagttgcc gttccacgtc gatctcgacc
     5401 tcgtcaccat caagccggtc gcccaggcgg caccgcacaa ggtggtgcgc ggcgaactga
     5461 aaccgcgcta gccagcgcct agacctcgat ctcgccgccc acggtgcgca ggtgctcacg
     5521 gaaggtcagc gcgggattcg cggcgcgggc ggccaggtag tcggagaaac gcacctgctg
     5581 ccgcaaggtc accccgtcga cgatgcggtc cgcctgccgt cgctcggtgt cccggatgcc
     5641 ctcggcgagg gtgaggagct cgtcgatacg gtccgccggc aggaacagca caccgtcgtc
     5701 gtcggccgcc acgaggtcac tgaagtcgac ggtccagtcg ccgatctggg cggagtcgag
     5761 cgcggcgctc gggcgggcat ccacgctcag tggcccggtg ggaatcgaac cgcagctgta
     5821 cagcgggatg ccgatggccg cgatgtcgac cgtgtcgcgg tggaggcccc agatcacgat
     5881 tcccgcgaga ccggcactcg cggcctcctg cgccatgagg tcgccgatgc agctctcgtc
     5941 gacgcggccc cggttgtcgg cgaccagcac gtctccgggc tgcgcgttct cgatcgcttc
     6001 gaggaacacg tcgacgctgc cgacgtgacg ggccggcagc acgcgaccga tgagtctcat
     6061 gcccgccgac agcgactgga tctgcgcggg cgcgcaccgc acttcgactc cggcccgcac
     6121 gcaggcatcg gcaaggtggg gagtggtcag ctcggcgaaa cgcgcggcga tatcgggcat
     6181 cagatcctct tctcgaacca gtggtgggcg tagggctcgt cgttgtaggc ctcaatctcg
     6241 cggtagccca gcgcgcggta gagcgcgatg gcctccgtga gagcacggtt ggtgtcgagc
     6301 ctgagggtgt ggatgccccg gctcgatccc tcggcttcga tgcggccgag catccgtcga
     6361 gccaacccga gccctcgaac ctcgctcgac acccacagcc gcttgacctc acccacaccg
     6421 tcggcgtgca acttgaggcc gacacagccg acaggtcgat cgtggagggt ggcgagcagc
     6481 accagccccg cgggcaaccg gaactgctcg tcggcggtcg gcagggtgac ggccgggtcg
     6541 aagcccgcct cgaacctgcg ggcgagttcg tcgaagtacg cggccatgct tgtgcggaca
     6601 gccgggcttg ccggatccgc aatggccacg gtgatcgccg atgcctcgag cagtcgactg
     6661 acagtcgcca tggccccggc gagttcgacg cgctgacggt cgcccagagg gtcgagcatt
     6721 gcgctcgcct gggcatcgga gagctcgtcg agggcggcgc gctcgcggag accggccggg
     6781 gtgagggcga cacggcgcac gcgcctgtcg tctccgtcgg tgctgaccgt ggcgagcccg
     6841 tcggtctcca acgagcgcag gatgcggctg aggtacccgg agtcgaggtc gagccgggcc
     6901 cgcagttcgc gcagctcggc accgtcaccg atctcccaca gcacgcggga gtggccgagg
     6961 gggcgtccgc gggagaggaa cgagtcattg agcgcgccga cgcgctgggt gaccgtgcgg
     7021 ttgaacctcc gcacgacgcc gacctgcacc tcgtccattt ctctgacttt agtcagagaa
     7081 agcgccagtc gccagtgggc gcctcacaat cgtcagtcgg cgacgcgcgt ggtcccctcc
     7141 gcggcgagtg tggcctcctc gatcagtgcc tcctccacgt cggcgaaggc gagcttcggc
     7201 acgaggaaga gcagcaccgc cgccacgagc gcgccgacgc cacagatcgt gaagaccgtg
     7261 aggtagccga gcaggctcga tgcggtcgcc gtgaccgcgc cggcggctcc ggcagcgagc
     7321 acgatcgcga aggtcgccga ggcgaaggat ccaccgatcg tcttggtcgt attcgtgagc
     7381 gcggtcgcga tgccggtctg cccgcgcggg gcggcagccg ccgcggccga cggcagggct
     7441 cccaccagcg caccggagcc gattcctgca atggccatgt tgacgaagac gccagcgacc
     7501 gaggtgtggt tgacgaggaa cagcagatag cccgtcgcca cgaagaaggt ggcgatgatg
     7561 agggcgatgc gaggactgaa ccatttcgac acccgcggga acagcagggc gcccacgatc
     7621 atcgacacga ggtacgcccc gatgatgtaa gagatctcgt cggccgccat gcccaggccg
     7681 tacccgttga tggggtcggt gccggcgaac gtggccagcg gtgcctgggc acccagcagg
     7741 ctgatgccga cgaggccggc ggtcagttgc accggccaca tgttcggctg cttcagcacg
     7801 cgcagatcga tcgcggggtc cttctgcttg agctcgtagc gactccacgg gatgaagatg
     7861 agcaccccca cgaccatcag caggtagcac caccaggcgg tcggcccatt gatgcgcagg
     7921 aaggtcagac cggacgtgat gaacagcagc ccgatggtca gaagaacgaa gccccacagg
     7981 tcgagcgagc ggcccggcag cggcttcgac tccggcaccc cgaacaggat cgcgaagaac
     8041 acgagggtca ccgcgacggc gggcaccatg agggtcgtct gcacgtcccc gccgagggcc
     8101 ttgaagatgc gccccgcgcc gagagcgccg atgatcgcac cggcctcgag ggcgaccacg
     8161 aggtatccgg cggcgcgcct cgtctgggat gcaccgcgac cggtgacgcg cccgcggtcg
     8221 aagatgaggg ccacctcgag cggcagccac acgacgtaga agccctgcag cgcccacgcg
     8281 atgaggaagg ttgtgaacga attggtgaac gccagcgccc aggtcgagcc ggcggtcagc
     8341 accgtcgcga tcagcaggat gcgcttgtgc ccgaacatgt caccgcactt ggcgagaatc
     8401 ggcaccacga tcgcgctcag gagcagttgc gctgcctcga accagttgaa gtcggcgtcc
     8461 ttgatgccca ggtgatcgac gaggtcgggg atgagcggaa catagaaacc ctgcaggatt
     8521 ccgctgacca gctcgacgag gaacaggaaa cccacgaggc cgaacgtgat agctccggcc
     8581 ttctctttca ccagtgacat gaacggatgc tacaaccgtc gcgctcccgc gggtgaacga
     8641 actcggctga ccggcgcgct agcgtagtgg catggccgac gacgaacgcg aagtgctcac
     8701 ctggagcgac ttcgggagcg catcgcgcga cctggcgttg gatgtgatcc gcagcggttt
     8761 cgaggccgaa gtcgtggttg ccatcgcccg gggtggactc ctgctcgcgg gcgggatcgc
     8821 ctacgcgctg gggctcaaga gttgcggtgc cgtcaacgcc gagttctaca cgggcgtggg
     8881 cacacgcctc gacaccccga tccttctgcc gcccttcct
//
LOCUS       DAOSOU010000073         8837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_7894_length_8837_cov_6.517308, whole genome shotgun sequence.
ACCESSION   DAOSOU010000073 DAOSOU010000000
VERSION     DAOSOU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7894_length_8837_cov_6.517308"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLW15_05580"
     CDS             complement(<1..356)
                     /locus_tag="PLW15_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOT33905.1"
                     /translation="MRKVFSLPDLGEGLTESEVVGWRVSVGDNVELNQIIADVETAKA
                     AVELPSPVAGVVAVLHAEAGQTVAVGAPLVTFETTADDSAASSGDDPDTARTDSPEAH
                     LVGYGARAETAVPRRRA"
     gene            complement(353..1366)
                     /locus_tag="PLW15_05585"
     CDS             complement(353..1366)
                     /locus_tag="PLW15_05585"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOT33906.1"
                     /translation="MSTLTLSKAIGAGLRRAMETDPKVVLLGEDIGKLGGVYRITDGL
                     QADFGERRVVDTPLAESAIIGAAVGLAYRGYRPVCEIQFDGFIYPAFDQIVSQVSRMH
                     YRTKGAVRMPITIRVPWGGGIGAAEHHSDSPEAYFTHTAGLRVVACSNPQNAYTMIRE
                     AIASDDPVLYFEPKRRYHLKGEVDLDAPLSSQSPGRSQVLIAGTDVTVVAYGALVQTA
                     LDAAAAAEQEGTSVEVIDLLSLSPIDYSPIAESVRRTGRLVVAHEAALTGGVGAEVCA
                     SITERCFEYLEAAPVRVTGHDIPYPPAKAEGHHIPDLDRLLDGIDCAMGRPNSRSGMG
                     AGS"
     gene            complement(1363..2424)
                     /gene="pdhA"
                     /locus_tag="PLW15_05590"
     CDS             complement(1363..2424)
                     /gene="pdhA"
                     /locus_tag="PLW15_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOT33907.1"
                     /translation="MQLLTPAGERVASPEYDPWVADVDDAQLRDLYRDMVVVRRIDTE
                     ATALQRQGELALWPPLLGQEAAQVASARALRSDDFVFTSYRENAVAYCRGAAPVELVS
                     VWRAVTATGWNPYSINMATPQVVIGAQTLHATGYALGIQADGADAVAVAYFGDGATSE
                     GDVSEALVFAAAYSAPVVFFCQNNHWAISEPVTLQTRGSLAARGLGFGIPSMRIDGND
                     VLAVTAATRIALDRARSGGGPTFIEAVTYRMGPHTTADDPTRYRPPAELEEWRGRDPI
                     ARLEAYLTSRDQLSDDFRAEVQAVADSTAAELRAGCIALPDPDPLSIFDHVYAEPHPA
                     LQQRREWYAHYLDGFEAAP"
     gene            2581..3048
                     /locus_tag="PLW15_05595"
     CDS             2581..3048
                     /locus_tag="PLW15_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT33908.1"
                     /translation="MAGYDQIDHNLLLALADDPRATVVALADRLGLARNTVQARMTRL
                     ERDAAFLSFERTINPVPLGYPIHAFIAVHLQQKILASVVHELAKIPEVVQAHGLSGPV
                     DLFVSVVCRDTADLFRVDDSILSIAGVERTETSLSMGELIPFRLRPLLERAPS"
     gene            complement(3045..3662)
                     /locus_tag="PLW15_05600"
     CDS             complement(3045..3662)
                     /locus_tag="PLW15_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT33909.1"
                     /translation="MAETWRATAKAHRRQQLLDAAAHLFAERGFNGVSIEDLGAAAGV
                     SGPALYRHFPSKEAMLGELLIGASRRLLEGCTRIIAEDAESQQALRDLVDFHVDFALS
                     ERDIIRIQDRELASLPPEVNREVRMLQRRYVQEWVALVARLSPTTPTAEIEVRVHAIF
                     GLLNSTPHNARLVDVDDARGVLAEMAITGMLGARTAVDTASLDTT"
     gene            3748..5355
                     /locus_tag="PLW15_05605"
     CDS             3748..5355
                     /locus_tag="PLW15_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT33910.1"
                     /translation="METLATGARPHSDDFVANDASQRALVSELRERLERVGRGGSEAS
                     RNRHSARGKLLPRDRIDYLLDRGSPFLEVGALAAYGMYDDDSPGAGIITGIGLVSGRL
                     CMIVCNDATVKGGSYYPMTVKKHLRAQEIASENLLPCIYLVDSGGAYLPLQDDVFPDR
                     EHFGRIFYNQSRMSARGIAQISAVLGSSTAGGAYMPAMSDETVIVRNQGTIFLGGPPL
                     VKAATGEVVSAEDLGGGVTHSRLSGVTDHLAENDEHALDIVRDIIAQLPPPAPRPYEL
                     SPVTEPTVNPAELYGVVPPELHVAYDVREVIARIVDGSQFHEFKAEYGQTLVTGFARI
                     HGHPVGIIANNGVLFSESALKGAHFIELCDQRKIPLVFLQNITGFMVGREYEAGGIAK
                     HGAKMVNAVASARVPKLTVVIGGSFGAGNYSMSGRSYSPRFLWMWPASRISVMGGPQA
                     AAVLSTIRRDQLDAKGDTWSAEDEVTFTQTLRDQYERQGNPYYSTARLWDDGIIDPAD
                     TRTVLGLSLEVCSRAPLADISYGVFRM"
     gene            5352..>6048
                     /locus_tag="PLW15_05610"
     CDS             5352..>6048
                     /locus_tag="PLW15_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOT33911.1"
                     /translation="MTVFDTVLIANRGEIACRIIATLRTLGIRSVAVYSDADAAARHV
                     SLADVAVRIGRASAADSYLNVERIVGAAVESGAQAIHPGYGFLAENAQFAAACAAAGI
                     VFIGPNIDALEIMGDKIRAKAQVTARGVPVVPGVARPGLTDHELTKAALEIGFPALIK
                     PSAGGGGKGMHVVWAPEELEDALAAARREAQSSFGDDTLFIERFLARPRHIEVQVLAD
                     GHGNVVHLGERECS"
     assembly_gap    6049..6148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6149..7519
                     /locus_tag="PLW15_05615"
     CDS             <6149..7519
                     /locus_tag="PLW15_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOT33912.1"
                     /translation="VLADGHGNVVHLGERECSLQRRHQKVIEEAPSPLIDETTRERIG
                     EAACESARSVGYLGAGTVEFIVSADAPDEFFFIEMNTRLQVEHPVTELVTGIDLVEWQ
                     LRIAAGEPLDFAQRDVELTGHAVEARVYAEDPTRGFLPTGGTVLELQEPTGAGIRVDS
                     ALVPGLAVSSDYDPMLAKVIAWAPDRASALQRLRAALRDTVVLGVGTNVEFLGLLLSA
                     PAVESGDLDTELIGRILPDLAFAEPAPDVFATAALIRHADGWRASEAGPWTTPNGWRM
                     GAAAPSVYRMRSVPGEVSATVRVWGSPSHLRVRVDDGDLRDRSVVLRGCQARVTIGEV
                     SATWVFAESGGDELYLSSQGVSWTLLETRGTRGESHDGDADPELRSPLPGAVVAISVT
                     DGQVVEAGAPIAIVEAMKMEHVLRAPVAGSVSLRVALGSQVAGDQIVATIVPVEEARV
                     LPEERP"
     gene            7516..7995
                     /locus_tag="PLW15_05620"
     CDS             7516..7995
                     /locus_tag="PLW15_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT33913.1"
                     /translation="MTEKRIVQRGLYYEEFELGVTYVHSPSRTVTEADNVLFTTLTMN
                     TQSLHLDAEWARTTEFGERLVNSMFTLSTLVGTSVAQLTQGTIVANLGIDNARFPHPM
                     RHGDTMTSESVVIAKRLSESRPGQGIVTLEHTARNQNGEVVAVATRNVLMLCAPEER"
     gene            7997..8794
                     /locus_tag="PLW15_05625"
     CDS             7997..8794
                     /locus_tag="PLW15_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOT33914.1"
                     /translation="MVLGPAILFCPGDRPDRFGKAAMAADAVILDLEDGVAPSDKQAA
                     RAAVAASDLDPDRTVVRVNAASTEEFARDLTAVASSGFRTIMLSKAERVADLEALAGY
                     SVIPLCETPIGVENAFALAEQTPVIALMWGAEDLVAALGGRSSRTADGRYRDVARYAR
                     ARVLVAAAAAGKQAYDAVHLDIADLAGLRDEAEDGAASGFVGTACIHPSQVETVREAY
                     RSSVEAVAWAERVLAAAAISAGVFAFEGRMVDEPVLKQARRILERRG"
BASE COUNT         1383 a         2893 c         3003 g         1458 t
ORIGIN      
        1 gcgcggcggc gcggaacggc ggtctcggcg cgggcgccgt accccacgag gtgggcttcg
       61 gggctgtcgg tgcgggcggt gtcagggtca tcacccgagg aggcagccga gtcatccgcc
      121 gtcgtctcga aggtgacgag gggcgcaccc accgcgacgg tctggccggc ttcggcatgc
      181 aacaccgcga cgacgcccgc gacgggcgac ggcagttcga cggcggcctt ggcggtctcg
      241 acgtcggcga tgatctggtt gagttcaacg ttgtcgccga ctgacacgcg ccatccgacg
      301 acctcggatt cggtgagtcc ctcgcccagg tcgggcaggc tgaagacctt cctcatgatc
      361 cggcccccat gccactgcgc gagttcggcc ggcccatcgc gcagtcgatg ccgtcgagga
      421 ggcggtcgag gtcggggatg tggtgaccct cggccttggc tggcggatag gggatgtcgt
      481 ggccggtgac ccgcacgggc gcagcctcga ggtactcgaa acagcgctcg gtgatgctcg
      541 cgcagacctc ggcgccgacg ccgccggtga gcgcggcctc gtgtgcgaca accagtcgtc
      601 cggtgcgccg aaccgactcc gcgatcggcg agtagtcgat cggcgagagt gagagcaggt
      661 cgatgacctc gacggaggtg ccctcctgct cggccgcagc cgcggcatcc agagcggtct
      721 gcaccaacgc cccgtacgcg acgacagtga cgtcggttcc ggcaatgagc acctgcgaac
      781 ggcccggtga ctggctggac aagggtgcgt ccaggtcgac ttcgcccttg aggtggtagc
      841 ggcgcttcgg ctcgaagtag agcacagggt catccgatgc gatggcctcg cgaatcatcg
      901 tgtacgcgtt ctgggggttc gagcaggcca cgactcgcaa gccggcggtg tgggtgaagt
      961 acgcctcggg cgagtcggag tggtgctcgg ccgcgccgat gccgccgccc cacggcaccc
     1021 ggatggtgat cggcatgcgc accgcgccct tggtgcggta gtgcatgcgc gagacctggc
     1081 tgacgatctg gtcgaaggcc gggtagatga acccatcgaa ctggatttcg cacacaggtc
     1141 gatagccgcg ataggcgagc cccaccgccg caccgatgat cgcggactcg gccagcggag
     1201 tgtcgacaac ccgacgctca ccaaagtcgg cctgcaggcc atcggtgatt cggtagacgc
     1261 cgccgagctt gccgatgtcc tcgccgagca gaaccacctt gggatcggtc tccattgcgc
     1321 gtcgaagccc cgctccgatg gccttgctca gggtcaacgt gctcacggcg cggcctcgaa
     1381 tccgtcgaga tagtgcgcgt accactcacg gcgttgttgg agcgcggggt gaggttcggc
     1441 gtagacgtgg tcgaagatac tcagtggatc ggggtcgggg agggcgatgc atccggcccg
     1501 cagctcagcc gccgtgctgt cggccacggc ctgcacctcg gcgcggaagt cgtcggagag
     1561 ttggtcacgg ctcgtcaagt aggcctcgag cctggcgatc ggatcgcgcc cgcgccattc
     1621 ctccagctcc gcgggcggtc ggtatcgcgt ggggtcgtcg gcagtcgtgt gcgggcccat
     1681 tcggtaggtg acggcctcga tgaaggtcgg cccgcccccg gatcgcgcgc gatcgagggc
     1741 gatccgcgtc gcggcggtga ccgcgagcac gtcgttgccg tcgattcgca tcgacgggat
     1801 gccgaacccg agaccgcggg cggccaggct tccccgggtc tgaagcgtga ccggctcgga
     1861 gatggcccag tggttgttct ggcagaagaa gacgacgggc gcgctgtatg cggccgcgaa
     1921 gacgagcgct tcgctgacat cgccttcgct cgtcgcgccg tctccgaagt aggcgaccgc
     1981 caccgcgtcg gcaccgtctg cctggatgcc caaggcgtaa ccggttgcgt gaagggtctg
     2041 ggctccgatg accacctgag gtgtcgccat gttgatcgag tacgggttcc accctgtcgc
     2101 cgtgaccgcg cgccacacgc tcacgagctc gacgggcgcc gcaccccggc agtaggcaac
     2161 ggcgttctcg cggtagctcg tgaacacgaa gtcgtcgctg cgcagcgcac gtgcggaggc
     2221 gacctgtgcc gcctcctggc cgagcagcgg gggccagagc gcaagctcgc cctgccgctg
     2281 aagcgccgtc gcctcggtgt cgatgcgacg aacgacgacc atgtctcggt agaggtcgcg
     2341 cagctgagcg tcgtcgacat cggccaccca cgggtcgtac tccggcgagg cgacgcgctc
     2401 accggcgggt gtcaggagtt gcacgggatc gggctcgtca gatgtgttca cgccgcgccc
     2461 ccccgtcatt ggtcgagtgc tcgcaacact aggttgcgtc gacactaagc tcaagagtgc
     2521 gcgctcatac tgagcattgt gctcactctg agcagatggt cggtgctact attgcgcact
     2581 atggcaggtt acgaccagat cgatcacaac cttctcctcg cgctcgccga cgatcctcgt
     2641 gcgactgttg tcgccctggc cgaccggttg gggctcgctc gcaacacggt tcaggcgcgg
     2701 atgacacggc tcgagcgcga cgctgcgttc ctgtcattcg agcgcaccat caaccccgtg
     2761 cctctgggat acccgatcca cgccttcatc gcggtgcacc tgcagcagaa gattctcgca
     2821 agcgtggttc acgagctcgc caagattccc gaggtcgtgc aggcccacgg gctcagcggc
     2881 ccggtcgatc tcttcgtgag cgtggtgtgc cgcgacacag ccgacctgtt ccgagtggat
     2941 gactcgatcc tgtcgatcgc gggcgtcgag cgcaccgaga catccctgtc gatgggggag
     3001 ctcatcccgt tccggctgcg gccgctgctc gagcgggcac cgagctaggt ggtgtcgagc
     3061 gaagcggtgt cgaccgccgt gcgcgcgccg agcatcccgg tgatcgccat ctcggcgagc
     3121 actccgcgcg cgtcatcgac gtcgacgagt cgcgcgttgt gaggcgttga attcagcagc
     3181 ccgaagatcg cgtgcactcg cacttcgatt tccgcggtgg gggtggtcgg gctgagccgg
     3241 gcgaccaggg cgacccattc ctgcacgtag cggcgctgga gcatcctcac ttctcgattc
     3301 acctcgggcg ggaggctggc gagctcgcga tcctggatgc ggatgatgtc gcgctcgctc
     3361 agcgcaaaat ccacgtggaa gtcgacaagg tctcgcaacg cctgctggct ttcggcatct
     3421 tcggcaatga tgcgcgtgca cccttccaga agacgccggc tcgcgccgat caggagctcg
     3481 ccgagcatcg cctccttgct cgggaagtgc cgatacaacg cggggccgct cacaccggcc
     3541 gctgccccga ggtcttcgat ggagaccccg ttgaatcctc gctcggcgaa cagatgtgct
     3601 gcagcgtcga gcagctgttg gcgacggtgc gccttggccg tcgcccgcca cgtctctgcc
     3661 atgcccccat ggtaccgatt ctggacaatt cagttaacga tcgctaacct aaactggatt
     3721 agcgcacact aactgagacg aggcgcgatg gagacgcttg cgacgggcgc ccgcccgcac
     3781 agcgacgatt tcgtcgcgaa cgacgcctct cagcgtgccc tggtcagtga gctccgggag
     3841 cggcttgagc gcgtcggacg cggaggctcc gaagcatccc gcaaccggca cagcgcgcgg
     3901 ggaaagctcc ttccccgtga ccggattgac tacctgctcg accgcggcag cccgtttctc
     3961 gaggtgggtg cgctcgcggc gtacggcatg tacgacgacg acagccccgg cgcgggcatc
     4021 atcaccggca tcgggctcgt gagcggtcgg ctgtgcatga tcgtgtgcaa cgacgccacc
     4081 gtgaagggcg gctcgtacta tccgatgacc gtcaagaagc acctgcgcgc gcaggagatc
     4141 gccagcgaga acctgctgcc ctgcatctac ctggtcgact ccggtggcgc ctacttgccc
     4201 ttgcaggacg acgtatttcc ggatcgggag cacttcggcc gcatcttcta caaccagtct
     4261 cggatgtcgg cccgcggcat cgcgcagatc tcggcggttc tcgggtcgag cacggccggc
     4321 ggagcctata tgcccgcaat gagtgacgag accgtgatcg ttcgcaacca gggaacgatc
     4381 ttcctcggcg gccctccgct cgtgaaggcg gcaaccggtg aggtcgtgtc cgccgaggac
     4441 ctcggtggtg gcgtcaccca ctcgcgcctg tctggcgtga ccgaccatct ggccgagaat
     4501 gacgagcacg ctctcgacat cgtgcgcgac atcatcgcgc agctgccgcc gcccgcgccg
     4561 cggccctacg agctcagccc cgtgaccgag ccgacggtca atcctgccga gctgtacgga
     4621 gttgtccctc ctgagctgca cgtggcctac gacgtgcgcg aggtgatcgc gcgcatcgtt
     4681 gacggcagcc agttccacga gttcaaagcc gagtacggcc agactctcgt caccgggttc
     4741 gcccgtatcc acgggcatcc ggtcgggatc atcgcgaaca atggcgtgct cttcagcgag
     4801 tccgccctca agggtgcaca cttcatcgag ctgtgcgatc agcgcaagat tccactggtc
     4861 ttcctgcaga acatcaccgg cttcatggtc ggtcgcgagt acgaagcggg cggtatcgcg
     4921 aagcacggcg cgaagatggt caacgccgtc gcctcggcgc gcgtgcccaa actcaccgtc
     4981 gtcatcggcg gctcgttcgg tgccggcaac tactcgatga gcggccggtc gtactccccg
     5041 cgattcctct ggatgtggcc ggcgagccgc atctcggtca tgggtggccc tcaggcggcg
     5101 gccgtgctgt cgaccatccg ccgcgaccag ttggacgcca agggcgacac gtggagcgcc
     5161 gaagacgagg tgaccttcac ccagaccctg cgcgatcagt acgagcgcca gggcaacccc
     5221 tactattcga ccgctcggct ctgggatgac ggcatcatcg acccggccga cacccgcacc
     5281 gttctcgggc tgtcgcttga ggtttgctcg cgggccccgc tggccgacat ctcctacggc
     5341 gtcttcagga tgtgaccgtg ttcgacaccg tactgattgc caaccgcggc gagatcgcct
     5401 gtcgcatcat cgcgaccctt cgcactctcg gcattcgttc cgtcgccgtc tacagtgacg
     5461 cggatgccgc ggctcgccac gtctctttgg ccgacgtagc cgtgcgcatc ggtcgcgcga
     5521 gcgcggccga cagttacctc aacgtcgagc ggatcgttgg tgccgccgtg gagagcggag
     5581 cgcaagccat ccatcccggg tacgggttcc tcgccgaaaa tgcgcagttc gcggccgcct
     5641 gtgcggccgc ggggatcgtc ttcatcggcc cgaacatcga cgccctcgag atcatgggcg
     5701 acaagatccg cgccaaggcc caggtgaccg cgcgcggggt gccggtcgtt ccgggagtcg
     5761 cacggccggg cctcaccgat cacgagctca cgaaggccgc gctcgagatc ggattccccg
     5821 cgctcatcaa gccgtcggcc ggcggcggcg gcaagggaat gcacgtcgtg tgggcccccg
     5881 aggaactcga ggatgcgctc gccgccgccc gccgcgaggc acagtcgtcg ttcggcgatg
     5941 acaccctctt catcgagcga ttcctcgccc gaccgcggca catcgaggtg caggttctcg
     6001 ccgatggcca cggcaacgtc gtacatctgg gcgagcgtga gtgctcgcnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tctcgccgat ggccacggca acgtcgtaca
     6181 tctgggcgag cgtgagtgct cgctgcagcg acggcaccag aaggtcatcg aagaagcccc
     6241 atcgccgctt atcgacgaga caacgagaga acgaatcggg gaggccgcgt gcgagagcgc
     6301 gcgcagcgtg ggctacctcg gcgcgggcac agtggaattc atcgtctcgg ccgacgctcc
     6361 cgatgagttc ttcttcatcg agatgaatac gcgcctgcag gtcgagcatc ccgtgaccga
     6421 gttggtgacg gggatcgacc tagtggagtg gcaactgcgc attgccgccg gcgagccgct
     6481 cgacttcgcg cagcgcgacg tcgagctgac cggccacgcg gttgaggccc gcgtctacgc
     6541 ggaggacccg acgcgagggt tcctccccac gggcggcacg gtgctcgagc tgcaggagcc
     6601 gactggcgcg ggcatccggg tcgacagcgc tctcgtcccg gggcttgcgg tctcgtccga
     6661 ctacgacccg atgctggcga aggtcatcgc gtgggcgccg gaccgggcat ccgctcttca
     6721 gagactgcgg gctgccctgc gcgacacggt agtgctcggc gtcggtacga acgtcgagtt
     6781 tctcgggctg ctgttgagcg cacccgcggt cgagagcggc gacctcgata ccgagctgat
     6841 cggccgcatc ctgcccgacc tggccttcgc cgagccggca ccggacgtgt tcgcgaccgc
     6901 ggccctgatt cgccacgctg acggctggcg ggccagcgag gccgggccgt ggacgacgcc
     6961 caacgggtgg cggatgggcg cagccgcacc cagcgtctac cggatgcgtt cggtgccggg
     7021 cgaggtgtcc gcgacggttc gcgtgtgggg atcgccgtcg cacctgcggg tgcgcgtcga
     7081 cgacggggat ctgcgggacc gctctgttgt gctgcgcggg tgccaggcgc gcgtgacgat
     7141 tggcgaggta tccgccacgt gggtcttcgc cgagtcgggc ggtgatgagc tctatctctc
     7201 gtcccagggt gtgagctgga cccttctcga gactcgtggc acgcggggtg agtcgcatga
     7261 tggcgacgcc gatccagagc tgcggtcgcc actgccgggc gccgtggtgg cgatctccgt
     7321 tacggatggg caggtggtcg aggcgggcgc accgatcgcg atcgtcgagg cgatgaagat
     7381 ggagcacgtg ttgcgcgcgc cggtcgccgg tagcgtatcc ctgcgggttg cgctcggctc
     7441 gcaggtcgcc ggcgaccaga ttgtggcaac gatcgtgccg gtggaagaag cgcgggtgct
     7501 gccggaggag aggccatgac ggagaaacgc atcgtgcagc gcggcctgta ctacgaggag
     7561 ttcgagctcg gcgtcaccta cgtgcacagc ccgtcgcgca ccgtgaccga agccgacaat
     7621 gtgctgttca ccaccctcac gatgaacact cagtcgctcc atctcgacgc ggaatgggct
     7681 cgcacaacgg agttcgggga gcgactcgtc aacagcatgt tcactctgtc gactctcgtc
     7741 ggaacgtccg tggcgcagct gacccagggc acgatcgtgg caaacctcgg catcgacaac
     7801 gcgcggttcc cccacccgat gcggcacggg gacacgatga cttccgagtc ggtggtgatc
     7861 gccaagcggc tgtcggagag ccgacccggc cagggaatcg tgaccctcga gcacaccgct
     7921 cgcaatcaga acggcgaggt cgtcgccgtg gcgacgagga acgtcttgat gctctgcgcg
     7981 cccgaggagc gctgaggtgg tgctcgggcc cgccatcctc ttctgcccag gggaccgccc
     8041 ggataggttc ggcaaggccg cgatggccgc ggatgccgtg atcctcgacc tcgaggatgg
     8101 tgttgcgccg agcgacaagc aggctgcacg ggcggcggtc gcggcatccg acctcgaccc
     8161 ggaccgcacg gtcgtgcgtg tgaatgcggc gagcaccgag gagttcgcgc gcgatctcac
     8221 agcagtcgcg tcgagcggtt tccgtacgat catgctctcg aaggcagagc gcgttgccga
     8281 ccttgaggcc ctcgcgggat acagcgtcat tcccctctgc gagacaccga tcggcgtcga
     8341 gaacgcgttc gcgctcgccg agcagacacc cgtgattgct ctcatgtggg gggctgaaga
     8401 cctcgtcgcc gcactcgggg gccgctcgag ccgaacagcc gacggacggt atcgggacgt
     8461 cgcccgctac gccagggcgc gggtgctcgt cgccgctgcc gctgccggta aacaggcgta
     8521 cgacgcagtc cacctcgata tcgccgacct ggccgggttg cgcgacgaag cggaggacgg
     8581 cgccgcgtcg ggtttcgtcg gcacggcgtg catccacccg tcccaggtcg agaccgtgcg
     8641 ggaggcttac cgctcgtccg tcgaggccgt cgcgtgggct gagcgcgtgc tcgcggcggc
     8701 ggcgatctcc gctggcgtgt tcgcgttcga gggacgcatg gttgacgagc ccgtgctcaa
     8761 gcaggcgcga cgcatccttg aacggcgggg ctaaggtgag ctaccgaccg cgcgggcaca
     8821 gcgcccacca gttcgaa
//
LOCUS       DAOSOU010000074         8737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8022_length_8737_cov_5.789219, whole genome shotgun sequence.
ACCESSION   DAOSOU010000074 DAOSOU010000000
VERSION     DAOSOU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8022_length_8737_cov_5.789219"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="PLW15_05630"
     CDS             complement(<1..1267)
                     /locus_tag="PLW15_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOT33915.1"
                     /translation="MRPRVHTASLGRWVDPADAWAALCEGADAAFWLDSGPNATTGTS
                     YLGVAARLSTQFDGPVLEWLRAQQYGVDTSEVGSGFALGWVGWLGYELRGETMGVAVG
                     RASRYPNAAWMYVDRCLAFDHERGEVRLLSLADIDDYRAHVLAALSSAEPRAVAEPRS
                     VVEPVETPAVTWAYSDEQYLDMIAACQAAIRDGDAYQLCLTTEARVDARPDPYAAYLA
                     LRASSPTHHGALLVAGDIAVLSATPEQFLSVSADGVIESKPIKGTRPRGLTGLADEEL
                     RFELESSEKERAENLMIVDLMRNDIGRVAAVGSVTVTSLLAVESYAHVHQLVSTVRGV
                     LADGLTGIDAIAACFPAGSMTGAPKRSATLILDSLEQRARGIYSGAFGYVGLDGAIDL
                     AMVIRSIVVDEAGSTVGTGGGITALSVPGE"
     gene            1279..2061
                     /locus_tag="PLW15_05635"
     CDS             1279..2061
                     /locus_tag="PLW15_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT33916.1"
                     /translation="MSVQSIFRWRDGALEPLEHCDMTLTVIEAADSWLVSDGLTLGLE
                     LHRTRFMTSIPRGRYLELDVDGFWDAAIAAIPREGDWFPRVELHSRLGGPRLVFRLRS
                     APERTRSAVLATWRGRDPRTVPRVKGPDLDSMLRIRTDVQKQGADEAVILTPDGYVVE
                     GAYSSLLWWRGDILCGPPAEFERVDSVTARSVLTLAAALGLDTHEEAVTPAELDGTEL
                     WALSALHGIRIATSWVDGPSLAEQPGRLATWRARLDALRRPI"
     gene            2071..2712
                     /locus_tag="PLW15_05640"
     CDS             2071..2712
                     /locus_tag="PLW15_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT33917.1"
                     /translation="MNDIILWLLDVVQSVDPVLRTALAGFGILLETSVLVGLVVPGDT
                     IVIIAATAVESPVQFVALVLAVISGALIGESIGFALGRFFGPRIRMSRLGRWLGERNW
                     VRAETFLKRRGGVAVFISRFLPVLHSLIPLTVGMSPMRYRTFIAWTLPATTIWTLAYV
                     SVGAFAAGTFRELLDRLHYAGYIFAGIIVVFAVVVWLVKRRLERAVDDGTPAE"
     gene            complement(2788..3696)
                     /locus_tag="PLW15_05645"
     CDS             complement(2788..3696)
                     /locus_tag="PLW15_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT33918.1"
                     /translation="MFTARNKGRLALGAVVVGAALALSGCATGDPLDSGSSSGASETI
                     VVGSQAYYSNEIIAEIYSQALENAGFTVERKFQIGQRDAYLPSLESGEVDLFPEYTGN
                     LLQFYSPDTTATTSDDVYAELQTALPDTLTVLDQAAATDQDSYNVTADFAAANNLKSL
                     ADLKNVTVPLTLGGNPELAERPYGPAGLLATYGVTVGFTATGDTTVEDLLAGTVNIAN
                     VYSADPRIQTDNLVTLADPEGLFLASNVVPLVNKDVADQIADVINAVQAKLTAEGLVA
                     LNVQSTVDQKSSAEIATQWLKDNGLI"
     gene            complement(3714..4448)
                     /locus_tag="PLW15_05650"
     CDS             complement(3714..4448)
                     /locus_tag="PLW15_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT33919.1"
                     /translation="MGLFLDALAWIFSPDRLTDNPSLPLLVLQHLFYTFVSVVIAALI
                     AVPLGYLIGHTGRGREIAVGISGAARALPSVGLILLLVLVIGVTQKPLAAFIAFVLLA
                     IPSVLAGAYSGLEQIDRRVIDAARAVGMTEWQILLRVEIPLGLPLLIGGLRAATLQVV
                     ATVTVAAYIGLGGLGLPIFAGLPLRRFDWMLGAALLIIVLALLLDGLFALLQHLVVPR
                     GVTAGRVLDVRSSPVGRRAVVGTPAD"
     gene            complement(4450..5127)
                     /locus_tag="PLW15_05655"
     CDS             complement(4450..5127)
                     /locus_tag="PLW15_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT33920.1"
                     /translation="MTWVLNNLPLIGRLTLEHIALAIPPIVIGFLLSIPLGFLAYRYR
                     LTRGVLLTVAGLLYTVPSLALFVVLPPLLGISYLSSLNLSIALTIYAVAIMSRSMADA
                     LTSVDPAIRQSATAVGFGSWRRFWTVEFPLAGPVILAGLRVTAVSTISLVTVGILIGV
                     ENLGYLFTNGFQRRIIPEILAGVVTVVVIALVVDYLLVLAGRLIMPWAPRRPGRVLRA
                     AQVAEGA"
     gene            complement(5124..5945)
                     /locus_tag="PLW15_05660"
     CDS             complement(5124..5945)
                     /locus_tag="PLW15_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33921.1"
                     /translation="MIEFRSVSKQFPNGARAVDDFSLVIPSRKTTVLVGSSGSGKTTL
                     LRMINRMVDPTSGVIEIDGESILTRDPVKLRRSIGYVMQNSGLLPHFKVIDNVATVPV
                     LNGVPRKQARAQALELLDTVGLDRELADRYPSQLSGGQQQRVGVARGLAANPNILLMD
                     EPFGAVDPIVRAELQQETIRLQRDLDKTVVFVTHDIDEAFLLGDQVVILEEGAKIAQV
                     GSPTEIMSNPASDFVADFIGVRQGKRALTIRETARGKILVDSEGRAAGVLSGEFS"
     gene            complement(6127..6774)
                     /locus_tag="PLW15_05665"
     CDS             complement(6127..6774)
                     /locus_tag="PLW15_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT33922.1"
                     /translation="MTAHDTMEHMPESLVALRNSPVQARSTARLTALLDAAAAVVDEI
                     GFERLTTAMVAERAGASIGTVYRYFPDRIALLQALAARNLERVLSRLADEVVDERHAD
                     WGSALEGAFDILVDAFRTEAGFRGLRVGDVIDLRPAPTERTFNSIVSNRLLDGMVARF
                     GVADTAETRFHFEAAMEVADALVARAFARDAQGDEAFIQAARDATSAMLASHFGD"
     gene            6854..7594
                     /locus_tag="PLW15_05670"
     CDS             6854..7594
                     /locus_tag="PLW15_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOT33923.1"
                     /translation="MCGRFVVAGASSDLVALFDVDLPADNLPAPSWNIAPTDPVSIVL
                     DSIPKAADDDIPVRRLEAARWGLVPGWAKDPKVGVTAFNARIETLGEKAHFKNAIRKR
                     RAIVPATGYYEWHTVGDIKTPHFIHLPDGELFTFAGLYEWWRNPAAADDAPDKWLLST
                     TIITRAATGELASIHDRMPLFLQPELIDEWLDPHTDEPRDVLEAAFAGGEELAERAEF
                     YAVGRAVGNVRNNSPELIEPAAEGPSAQ"
     gene            7611..8660
                     /locus_tag="PLW15_05675"
     CDS             7611..8660
                     /locus_tag="PLW15_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HOT33924.1"
                     /translation="MARPGAPVTPAGAPKQIMHRAARIEDAFHRWRERRGRRRGLTTT
                     IIPYAGYGAPGWARVLCRVVLTRGGDAPKQRAAKVRGWRSFFSIPVDDVEVTISIGSV
                     ERRVRPERGGIVDVRIPVDLRTGWRKVRLRTDDTEPVTTKVFIVDPDAPFGIVSDVDD
                     TVMVTALPRPFLAAWNTFVLDEHARTPVAGMAVLYERLTRANPGAPVIYLSTGAWNVA
                     PTLTRFMARNLYPRGALLLTDWGPTHDRWFRSGQDHKRSSLERLAQEFPGMKWLLIGD
                     DGQHDESIYGDFAAAHPDKVAGVAIRQLSPGEAVLAGGRSKAEHEAGSAVPWFYSPDG
                     AGLSKQLVAAGLLTL"
BASE COUNT         1499 a         2885 c         2966 g         1387 t
ORIGIN      
        1 cctcccccgg gacggaaagc gccgtgatgc ccccgccggt gcccacagtc gagcccgcct
       61 cgtcaacgac gatcgaccgg atgaccatgg cgaggtcgat cgccccgtcg agtcccacat
      121 agccgaacgc ccccgagtag atgccgcgcg ctctctgttc aagggagtcg aggatgaggg
      181 tcgcggatct cttgggcgcc cccgtcatgg agccggcagg gaagcacgcg gcaatggcgt
      241 cgatgcccgt gagtccgtcg gcgagcacac cgcgcaccgt gctcaccagc tggtgcacgt
      301 gggcgtagct ctccaccgcc agaaggctgg tgaccgtgac ggagccgacg gcggcgaccc
      361 gaccgatgtc gttgcgcatg aggtcgacga tcatgaggtt ctcggcgcgc tccttctcac
      421 tcgactccag ctcgaagcgc agctcctcat cggccaggcc cgtgagcccc cggggccggg
      481 tgcccttgat cggcttcgac tcgatcacgc cgtcggccga gacgctgagg aactgctccg
      541 gcgtggcgct gagcaccgcg atatccccgg caacgagcag tgccccgtgg tgggtggggc
      601 tggatgcccg gagcgcgagg taggccgcgt aggggtcggg acgggcatcc actcgcgcct
      661 cggtcgtcag gcacaactgg taggcgtcgc cgtcgcggat cgccgcctga caggccgcga
      721 tcatgtcgag atactgctca tcggagtagg cccaggtgac cgccggggtc tcgacaggct
      781 cgaccaccga acgcggctcg gccaccgcgc gcggctcggc cgacgagagc gctgcaagca
      841 catgcgcgcg gtagtcatcg atgtcggcga gggacaacaa ccgcacctcg ccgcgctcgt
      901 ggtcgaaggc gagacagcgg tccacgtaca tccaggcggc gttcgggtag cgggatgccc
      961 gcccgacggc aacgcccatg gtctcgccgc gcagctcgta cccgagccag cccacccacc
     1021 ccagagcgaa gccggagccg acctcagacg tgtcgacgcc gtactgctgc gcgcgcaacc
     1081 actccagcac cggcccatcg aactgcgtcg agaggcgggc agcaacgccg aggtagctgg
     1141 tgccggtggt cgcgttcggt ccgctgtcga gccagaaagc ggcatccgcg ccctcacaca
     1201 gggcggccca ggcgtcggca gggtcgaccc accgaccgag ggacgccgtg tgtacccgag
     1261 gacgcatacc ctaaccctat gagtgtgcag tcgatcttcc ggtggcgtga cggagcactc
     1321 gagccgctcg agcactgcga catgaccctc acggtgatcg aagccgccga ctcgtggctg
     1381 gtgagcgatg ggctcacgct cggtctcgag ctgcaccgca cgcggttcat gacgtcgatc
     1441 cctcgcggcc ggtatctcga actcgacgtc gatggcttct gggatgccgc aatcgccgcg
     1501 atcccgcgcg agggtgactg gtttccccgg gtcgagctgc actcgcgcct cggcggcccg
     1561 cggttggtgt tccggttgcg ctccgccccc gaacgcaccc gatcggccgt gctggcgacc
     1621 tggcgtggac gggatccgcg aacggtgccg cgagtgaagg gacccgacct ggactcgatg
     1681 ctgcgcatcc gtaccgacgt gcagaagcag ggcgccgacg aagcggtgat cctcacgccc
     1741 gacgggtacg tggtcgaagg tgcctacagc tcgctgctgt ggtggcgcgg agacatcctg
     1801 tgcggcccac cggccgagtt cgagcgggtc gacagcgtca cggcgcgcag cgtgctcacc
     1861 ctcgccgccg cgctcggcct ggacacgcac gaggaggccg tgactcccgc cgaactggac
     1921 ggcaccgaac tctgggcgct gagcgcgctt cacggcattc ggatcgctac gtcctgggtc
     1981 gatggcccgt cactcgccga gcaaccgggt cggctggcaa cttggcgtgc acgtctggat
     2041 gcgctgcgcc gcccgatcta gggtcgaagc atgaacgaca tcattctgtg gctgctcgat
     2101 gtcgtccagt ccgttgaccc cgtgctgcgc acggcactcg ccggcttcgg aatcctgctc
     2161 gagacctcag tgctcgtggg gctcgtcgtg cccggtgaca cgatcgtcat catcgccgca
     2221 acggcggttg agtcgccagt gcagttcgtc gccctcgtgc tcgcggtgat ctcaggagcc
     2281 ctcatcgggg agagcatcgg gttcgcgctc ggtcggttct tcggtccccg catccggatg
     2341 tcgcggctgg ggcgctggct cggtgaacgc aattgggtgc gcgccgagac gttcctcaag
     2401 cggcgcggag gtgttgccgt gttcatctcg cgattcctgc ccgtgctgca ctcgctcatc
     2461 ccgctcaccg tcggcatgag cccgatgcgg taccgcacgt tcatcgcgtg gacgctgccc
     2521 gcgaccacca tctggacgct cgcctatgtg tctgtggggg ccttcgccgc tggcaccttt
     2581 cgcgagctgc tcgatcgcct gcactatgcc ggctacatct tcgcgggaat catcgtcgtg
     2641 ttcgccgtcg tggtgtggct cgtcaagcgc cggctcgagc gcgccgtgga cgacggaacg
     2701 ccggctgagt agcagcaaga ctgctgccgc cactcaaccg gcgttggggt ttcgatacgg
     2761 cgcttcgcgc ctactcaacc tgccttacta gatgaggccg ttgtccttga gccactgggt
     2821 ggcgatctcg gcggaggact tctggtcgac ggtggactgc acgttcaacg cgacgagtcc
     2881 ttcggcggtc agcttggcct gcacggcgtt gatgacgtcg gcgatctggt cggcgacgtc
     2941 cttgttgacc aacggcacga cgttggaggc caggaacagg ccctccgggt cggcgagggt
     3001 gacgaggttg tcggtctgga tgcgcgggtc agcgctgtag acgttggcga tgttgacggt
     3061 gccggcgagg aggtcctcga cggtggtgtc accggtggcg gtgaagccga cggtcacccc
     3121 gtaggtggcc agaagacctg ccggaccata gggacgctcg gcgagctccg ggttgccgcc
     3181 cagagtcaac ggcacggtca cgttcttcag atcggccaga gacttcaggt tgttggcggc
     3241 cgcgaagtcg gccgtcacgt tgtaggagtc ctgatcggtt gccgccgcct ggtcgagcac
     3301 cgtcagggtg tcgggcagag cggtctgaag ttccgcgtac acatcgtcgg aggtggtggc
     3361 ggtggtgtcg gggctgtaga actgcagcag gttgccggtg tactcgggga acaggtccac
     3421 ctcaccggac tccaacgacg gcaagtatgc gtcacgctga ccgatctgga acttgcgctc
     3481 caccgtgaag cccgcgttct ccagagcctg cgagtagatc tccgcaatga tctcgttcga
     3541 gtagtacgcc tgcgaaccca ccacaatcgt ctccgacgca cccgaggacg aacccgagtc
     3601 caacggatca ccggtcgcgc accctgacag ggccaatgcc gccccgacca cgaccgcacc
     3661 cagtgcgagc cggcccttgt tcctagctgt gaacatctct atttccttct cattcagtcg
     3721 gcgggggtcc ccaccaccgc acgccgcccc actggcgacg aacggacatc cagaacgcga
     3781 cccgcggtca ctccgcgggg cacgacgagg tgctggagca gcgcgaagag gccgtccaga
     3841 agcagtgcga gcacgatgat cagaagtgcg gcaccgagca tccagtcgaa tcgacgcagg
     3901 ggaagacccg cgaagatcgg gagcccgagg ccgccgagcc cgatgtaggc cgcgaccgtc
     3961 acggtggcca cgacctgcag cgtcgccgcc cgcagcccgc cgatcagcag tggcaggccg
     4021 agcgggatct cgacgcgcag caggatctgc cactctgtca tgcccaccgc gcgggcggca
     4081 tcaatcacgc ggcggtcgat ctgctcgagg cccgagtagg cgccggcgag cacggaggga
     4141 atcgccagga gtacgaatgc gatgaatgca gccagcggtt tctgggtcac gccgatgacc
     4201 agcacgagca ggagtatgag cccgaccgac ggcagcgcgc gcgccgcccc cgagatgccg
     4261 accgcgatct cgcggccgcg accagtgtgc ccgatcagat acccgagtgg aacggcgatc
     4321 agtgcggcga tcaccaccga cacgaacgtg tagaagaggt gctgcaggac cagcaacggc
     4381 agtgacgggt tgtcggtgag gcgatccggg gagaagatcc aggcgagagc gtcgaggaat
     4441 agacccatgt cacgccccct cggcgacctg tgcggcgcgc agcacccggc cgggccgtcg
     4501 aggcgcccac ggcatgatga ggcgcccggc gagcacgagc aggtagtcga ccacgagcgc
     4561 gatgaccacc acggtcacca ctcccgcgag aatctcgggg atgatgcgcc tctggaaccc
     4621 gttcgtgaac agatacccga ggttctcgac accgatgagg atgccgaccg tgacgaggct
     4681 gatcgtgctg accgccgtga cccgaagtcc cgcgaggatc accggcccgg caagggggaa
     4741 ctcgacagtc cagaatcgac gccagctgcc gaacccgacg gcggtggccg attggcgaat
     4801 cgcggggtcg acggaggtga gggcatccgc catcgacctg gacatgatgg ccacggcata
     4861 gatcgtgaga gcgatcgaca ggttgaggga gctgagatag ctgatgccca gcagcggcgg
     4921 gagcacaacg aacagggcga gcgagggaac cgtgtagagc aggccggcca cggtgagcag
     4981 gacaccgcgg gtgagccggt agcgataggc gagaaatccc agcgggatcg acagcaggaa
     5041 tccgatcaca atgggcggaa tcgccagcgc tatgtgctcg agcgtgagac gcccgatgag
     5101 cggcaggttg ttcagcaccc aggtcacgag aactccccgg agagcacacc cgcggcccga
     5161 ccctcggaat cgacgaggat cttgccgcga gccgtctcgc ggatcgtgag cgcacgcttg
     5221 ccttggcgca ccccgatgaa atcggcgacg aagtcagacg cggggttgga catgatctcg
     5281 gtggggctgc ccacctgggc gatcttggca ccctcctcga gaatgacgac ctgatcaccc
     5341 agaaggaatg cctcgtcgat gtcgtgagtc acgaagacga ccgtcttgtc caggtcgcgc
     5401 tgcagccgga tggtctcctg ctgaagctcg gcgcgcacga tcgggtcgac ggcaccgaag
     5461 ggctcgtcca tgagaaggat gttggggttg gccgcgagcc cccgtgcgac accgacccgc
     5521 tgctgctggc cgcccgagag ctggctcggg tagcggtcag cgagctcgcg atcgaggccg
     5581 acggtgtcga gcagttcgag cgcctgcgct cgagcctgct tgcgcggcac tccgttgagc
     5641 acgggaaccg tggcgacgtt gtcgatcact ttgaagtgcg gcagcaggcc ggagttctgc
     5701 atgacgtagc caatactgcg tcgcagtttc acagggtcgc gggtgaggat gctctcaccg
     5761 tcgatctcga tgacgcccga cgtgggatcg accatgcggt tgatcatgcg cagcagagtc
     5821 gtcttgccgc tgcccgacga cccgaccagg accgtggtct tgcgggacgg aatgacgagg
     5881 ctgaagtcgt cgaccgcgcg agcaccattg gggaactgct tcgacaccga acggaactcg
     5941 atcatgggta atacggctca ttcctcgtgc ccgaacacga atcgacactt gccagtacgc
     6001 tgacgctgat ctttcgacct actcagggca caacagtatt cggagccacc ggcattccgg
     6061 attggcatta cgaagttctt acgaccgacc cagggtcgcg gggatgccta cctgcggtcg
     6121 gtccgctcag tcgccgaagt gtgaggcgag cattgcactg gttgcgtcgc gggccgcctg
     6181 gatgaatgct tcgtcgccct gagcgtcacg ggcgaaggca cgagcgacga gagcatcggc
     6241 gacctccatg gccgcctcga agtggaaacg ggtctcggcg gtgtctgcca ccccgaatcg
     6301 cgcaaccatc ccgtcgagca ggcggttgga cacgatcgag ttgaaggtgc gctcggtggg
     6361 tgcggggcgc aggtcgatga cgtcgccgac ccgcagtccc cggaacccgg cctcggtgcg
     6421 gaacgcatcg acgaggatgt cgaacgctcc ctcgagagcg ctgccccagt ccgcgtggcg
     6481 ctcgtcgacg acctcgtcgg cgagtcggct gagcacgcgc tcgaggttgc gcgcggcgag
     6541 ggcctgcaga agggcgatcc ggtcggggaa gtagcggtag acggtaccga tggacgcacc
     6601 agcgcgttcg gcaaccatgg ccgtggtgag cctctcgaag ccgatctcgt cgactacggc
     6661 tgccgcggca tccagcagcg cggtgaggcg ggcggtgctt ctcgcctgca ccggcgagtt
     6721 gcgcagggcc acgagtgatt cgggcatgtg ttccatggtg tcgtgcgcag tcacggccat
     6781 ctccttcagt gttcgaaaca tattgtagct cgcgacatga ggaagtgtcg gcggaaactg
     6841 tgagaatgtt ctcatgtgcg gtcgattcgt cgtagcaggc gcgagcagcg atctggtggc
     6901 cctgttcgac gtcgatcttc ccgccgataa cctgccggcg ccgtcgtgga acatcgcccc
     6961 gaccgatccc gtgtcgatcg tgctcgactc gattcccaag gccgcggacg acgacatccc
     7021 tgtacgtcga ctggaagccg cgcgctgggg tctcgtgccc ggatgggcga aggatccgaa
     7081 ggtcggggtg accgccttca acgcccgcat cgagacgctg ggcgagaagg cgcacttcaa
     7141 gaacgccatt cgcaagcgcc gggccatcgt gcccgcgacc gggtactacg agtggcacac
     7201 cgtgggagat atcaagactc cccatttcat tcatctgccc gacggcgagc tcttcacgtt
     7261 cgctggcctc tacgagtggt ggcgcaaccc cgctgcggca gacgacgccc cggacaagtg
     7321 gctgctgtcg accacgatca tcacccgcgc ggcgacgggt gagctcgcga gcatccacga
     7381 tcgcatgccc ctgttcctgc agcccgagct gatcgatgag tggctcgacc cgcacaccga
     7441 cgagccacgg gatgttctcg aggccgcctt cgcgggcggc gaggagctcg ccgagcgtgc
     7501 cgagttctat gccgtggggc gcgcagtggg caatgtgcgc aacaactcgc ccgaactcat
     7561 cgagcccgcc gcagaaggcc cgtcggcaca atagctgaca gactgggtca atggctcgac
     7621 ccggcgcacc cgtgaccccc gccggggcgc cgaagcagat catgcatcgc gcggcgcgca
     7681 tcgaggatgc gttccaccgc tggcgtgagc gccgcggccg ccgacgcgga ctcaccacca
     7741 cgatcatccc ctacgccgga tacggagctc ccggctgggc tcgcgtgctc tgccgcgtgg
     7801 tgctgacccg gggcggcgac gcacccaagc agcgggctgc gaaggtgcgg ggctggcgca
     7861 gcttcttcag cattcccgtt gacgatgtcg aggtgacgat ctcgatcggt tccgtcgagc
     7921 gccgggtgcg cccggaacgt ggcggcatcg tcgacgtgcg catccccgtc gacctgcgaa
     7981 cgggatggcg caaggtacgc ctgcgcaccg acgacaccga gcccgtgacg accaaggtct
     8041 tcatcgtcga ccccgatgcc ccgttcggca tcgtctccga cgtggatgac acggtcatgg
     8101 tcaccgcgct gccccgcccc ttcctcgccg cctggaacac cttcgtgctc gacgagcacg
     8161 cgcgcacccc cgtcgccgga atggcggtgc tctacgagcg gctcacccgg gcgaacccgg
     8221 gggcgccggt gatctacctc tccaccggag cgtggaatgt tgcgccgacc ctcaccagat
     8281 tcatggcgcg caacctctac ccccgcggcg cactgctcct gaccgactgg gggccgaccc
     8341 atgatcgctg gttccggagc ggccaggacc acaaacgatc gagcctcgag cgactggcgc
     8401 aggagttccc cggcatgaag tggctgctca tcggcgacga cggccagcat gacgagagca
     8461 tctacggcga cttcgcggcg gcgcatcccg acaaggtcgc tggcgtggcc atccgccagc
     8521 tgagcccggg agaggccgtg ctcgccggtg gccgcagcaa ggccgagcac gaggccgggt
     8581 cggcggttcc gtggttttac tcccccgacg gcgcggggct gtcgaagcaa ctcgtggcgg
     8641 cgggccttct cacgctctga gcgcacagct ccccgacgac acgggctgag aggatggacg
     8701 gatggcactg cgcatcgagg actacgcgat gatcggc
//
LOCUS       DAOSOU010000075         8634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8134_length_8634_cov_5.351323, whole genome shotgun sequence.
ACCESSION   DAOSOU010000075 DAOSOU010000000
VERSION     DAOSOU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8134_length_8634_cov_5.351323"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="PLW15_05680"
     CDS             complement(<1..1407)
                     /locus_tag="PLW15_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOT33925.1"
                     /translation="MSDTEKPTLLVIDGHSLAFRAFYALPLDSFTTRDGQHTNAIHGF
                     LSMLILLLQNEKPTHLAVAFDISRYSFRTREYPEYKGTRGETPQEFVGQVPLLEEALK
                     AMGVWTVTKEDYEADDILATLATRGAAEGYEVLVVSGDRDAIQMVKPGVTLLYPNARG
                     VSELKRYDRAAVVERYGIEPEQYPDIAALVGETSDNLIGIDKVGEKTAVKWITAYGSL
                     DGVLEHADEITGVVGEKLREQKDRAIRNRKLNHLVTDVDLPFGPADLLRKPIDPVAVR
                     EVFDRLQFKTLLERVLKIAAAEQGADFVADAAPVVSGVPVIRKLVDEELGKWLSDQSR
                     NGQNALGLRIASANGQVTGFGIGAADETAYVPWAPGRPDYVALESWLASDAPKVMTGS
                     KAQLKIASQSGLVVRGIAYDTILAGWLLKPGSKAETLESQTEYYLGETLQQSDPNQLV
                     PETEPASPATEAWYSLRLS"
     gene            1453..1890
                     /locus_tag="PLW15_05685"
     CDS             1453..1890
                     /locus_tag="PLW15_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOT33926.1"
                     /translation="MPNVTILPPGLDPELVERLTGTNGGELTTKMGVEYVSLSAEHSV
                     ARMPVEGNRQVVGILHGGAHVVLGESLGSISSAIHAGPGRVAMGIEINATHSRAISEG
                     WVTATCDALVLGRTLATHEIVIRDDEGRRLSTVRMTNILRDKN"
     assembly_gap    1928..2027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2038..2643)
                     /locus_tag="PLW15_05690"
     CDS             complement(2038..2643)
                     /locus_tag="PLW15_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT33927.1"
                     /translation="MTDQDAATTVPRRVVVAEDESLIRMDIVEILRDNGFEVVGEAAD
                     GETAVALATELRPDLVIMDVKMPQLDGISAAERLSKNHIAPVVLLTAFSQKELVERAS
                     EAGALAYVVKPFTPNDLLPAIEIALSRYTQIITLEAEVADLVERFETRKLVDRAKGLL
                     NEKMGLTEPEAFRWIQKASMDRRLTMHDVAQAIIEQLSAKK"
     gene            2683..2765
                     /locus_tag="PLW15_05695"
     tRNA            2683..2765
                     /locus_tag="PLW15_05695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2716..2718,aa:Leu,seq:caa)
     gene            complement(2818..4260)
                     /gene="pyk"
                     /locus_tag="PLW15_05700"
     CDS             complement(2818..4260)
                     /gene="pyk"
                     /locus_tag="PLW15_05700"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT33928.1"
                     /translation="MRRAKIVATLGPATSSYENIRAIIDAGVDVARMNLSHGSYAVHE
                     EVYANVRKAAEDSGRAVAVMVDLQGPKIRLGKFANGPHELAVGDIFKITTEDILGTKE
                     IVGTTFKGLPDDVAAGDFLLIDDGKVKVRVVGVDGPVVTTEVVVAGAVSNNKGINLPG
                     VAVNVPALSEKDEDDLRWGLKLGADLIALSFVRNAEDIVRVHEIMAEEGRKVPVIAKI
                     EKPQAVDHLESIIDAFDAIMVARGDLGVELPLEAVPIVQKRTVELARRMAKPVIVATQ
                     MLESMISSPVPTRAETSDVANAILDGADAVMLSGETSVGEYPVITVQTMARIVSSTED
                     HGLERIPPLGTKPRTQGGAITLAAAEVADFVDAKFVCVFTESGDAARRMSRLRFRVPM
                     KAFTPDPAIRRRMALIWGIESFVVERVNHTDAMYRQVDEVLLREGLAEIGDKVVVISG
                     SPPGISGSTNDMRVHIIGDAINAAAPAWQD"
     gene            complement(4326..5783)
                     /locus_tag="PLW15_05705"
     CDS             complement(4326..5783)
                     /locus_tag="PLW15_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOT33929.1"
                     /translation="MADPKGFLKVQERETPVRRPVSLRLMDWKEVYEQQDPAQLRRQA
                     GRCMDCGIPFCHQGCPLGNLIPEWNDLTWRGEGAQAIERLHATNNFPEFTGRLCPAPC
                     ESACVLGINQPAVTIKQVEVSIIDEAFAKGWVTPHPPERLTGKTVAVVGSGPAGLAAA
                     QQLTRAGHTVAVFERDDRIGGLLRYGIPDFKMEKKHLESRLAQMQAEGTRFRAGVEIG
                     VDITWDDLKSRYDAVIVATGATVPRDLPIPGRDLMGVHFAMEYLVQANKVGAGDVVAN
                     QITAEGKHVVVLGGGDTGADCIGTAHRQGAFSVTNLAIGIQPPSERPEHQPWPMAPTL
                     FEVSSAHEEGGERVYLASTVEFLANEAGEVRAIRVAETEYLDGRRVPKSGTEREIPAD
                     LVLLALGFTGPEARTLEDQLPVPFGEKGTIERDGQYETSESGVFVAGDAGRGQSLIVW
                     AIAEGRAAAAAVDKYLEGDTQLPFPVRPTDHAISI"
     gene            complement(5776..>8634)
                     /locus_tag="PLW15_05710"
     CDS             complement(5776..>8634)
                     /locus_tag="PLW15_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOT33930.1"
                     /translation="ELAKIQHIDPRAGSQLSTTIRGLYRVDKGPKAMEKRLAKMCEEV
                     DEAIANGAEFIVLSDRDSNKDLAPIPSLLMLATVHHHLIREENRMKVGLIVEAGDVRE
                     VHHVALLVGYGASAINPYLAMETAEELVRSGMIQGITPEKAVKNIIKALGKGVLKIMS
                     KMGISVVGSYAGAQAFEAVGLSQEFVDQYFTGTSSLLGGVGMEVIAAESAARHSSAYP
                     EDGATSVHERLATGGEYQWRREGPPHLFNPDTVFRLQHATRARRYDIFREYTKLVDDQ
                     AENLMTLRGLFRIRSGERRPVPIDEVESIESIVKRFNTGAMSYGSISQEAHETLAIAM
                     NQLGARSNTGEGGEDVARLLDPERRSAIKQVASGRFGVTSMYLTHATDIQIKMAQGAK
                     PGEGGQLPAGKVYPWIARTRHGTAGVGLISPPPHHDIYSIEDLKQLIFDLKRSNPDAR
                     VHVKLVSQSGIGAVAAGVTKAKADVVLVSGHDGGTGASPLNSLKHAGTPWELGLAETQ
                     QTLMLNGMRDRVVVQVDGQMKTGRDVIIGALLGAEEYGFATAPLVVSGCILMRVCHLD
                     TCPVGVATQNPELRARFSGKPEFVVNFFEFLAQEVREYLAELGFRTLEEAIGHHELLD
                     VDRAVQHWKADGLDLSPILVGPEFSDDEPRTNRVSQEHELEKHFDRQLIELSVDALDN
                     GTPVVIDLPIRNTERAVGTMLGHEVTLRHGENGLPTGTIEVRLTGTAGQSFGAFMPSG
                     ITLRLEGDSNDYVGKGLCGGEIVIRPHRASLFKAEKNVIAGNVIGYGATQGSMFIRGI
                     VGERFLVRNSGATAVVEGVGDHALEYMTGGLAVILGGTGRNLGAGMSGGTAYIYDLRT
                     ERVNPESLSSGELELLPLGSADVEILRDLLQRHRAETDSTVAAGMLDELDAAVARFTK
                     VLPRDYAAVLQTRETAINEGLDPDGDVVWSRIMEVTGG"
BASE COUNT         1392 a         2772 c         2843 g         1527 t
ORIGIN      
        1 cgataggcgc agcgagtacc aggcctcggt ggccgggctg gcgggctctg tctccggcac
       61 gagctggttt ggatccgact gctgaagcgt ctctccgagg tagtactcgg tctgcgactc
      121 gagagtctct gccttcgacc ccggcttgag aagccagccg gcgagaatcg tgtcgtaggc
      181 gatgccccga accacgagac cggactggct ggcgatcttc agttgcgcct tggagccggt
      241 catgaccttg ggtgcgtcgc tggcgagcca cgactcgagt gccacatagt ccggacgacc
      301 gggagcccag ggcacgtacg cggtctcgtc tgccgcaccg atgccgaagc cggtgacctg
      361 tccattcgcg ctggcgatcc gcagtccgag cgcattctgg ccattcctgg attggtcgct
      421 gagccacttg ccgagctcct catcgacgag cttgcggatc accggcacac ccgagacgac
      481 gggtgcagca tccgcgacga agtcggcacc ctgctcagcg gcggcgatct tgagcacccg
      541 ctccagcaga gtcttgaact ggagccggtc gaacacctcg cgcacagcga ccggatcgat
      601 gggcttgcgc agcagatcgg ccgggccgaa cggcagatcc acatcggtga cgaggtggtt
      661 cagcttgcga ttgcggatgg cacggtcctt ctgctcccgc agtttctccc ccacgacgcc
      721 cgtgatctca tcggcgtgct cgagaacccc atcaaggctg ccgtacgcgg tgatccactt
      781 cactgccgtc ttctcaccga ccttgtcgat gccgatgagg ttgtcggatg tctcccccac
      841 gagcgcggcg atgtcggggt actgctccgg ctcgatgccg tagcgctcga cgacggcggc
      901 gcggtcgtag cgcttgagct cggagactcc gcgcgcgttc gggtacagca gtgtcacgcc
      961 gggcttgacc atctggatgg cgtcccggtc gcccgatacg acgagaacct cgtaaccctc
     1021 ggctgctccg cgagtcgcga gcgttgcgag gatgtcgtcg gcctcgtagt cctccttcgt
     1081 gaccgtccag acgcccatgg ccttcagtgc ctcttcgaga aggggaacct ggccgacgaa
     1141 ctcctgcggc gtctcgccgc gcgtgccctt gtactccggg tactcgcggg tgcggaagga
     1201 gtatcgggag atgtcgaagg cgacggccag atgtgtcggc ttctcgttct gcaagagcag
     1261 gatgagcatc gacaggaagc cgtgaatcgc attggtgtgc tggccgtccc gcgtcgtgaa
     1321 gctgtccagc ggcagcgcat agaaggccct gaaggcgagc gagtggccgt cgatgaccag
     1381 aagggtaggc ttttcggtgt ccgacacgga gccagcctag ccgcggcatc cgacagacct
     1441 acgatcgatt gggtgccgaa cgtgaccatc cttcctcccg gactcgaccc cgaactcgtc
     1501 gaacgtctca ccggcaccaa cggtggcgaa ctgaccacca agatgggcgt ggagtacgtc
     1561 tcgctgtcgg ccgagcactc ggtcgcgcgg atgcccgtgg agggcaacag gcaggtggtc
     1621 ggcatcctgc acggcggcgc ccacgtggtg ctgggtgagt cgctcgggtc catctcgtcg
     1681 gcgatccatg ccgggccggg ccgcgtggct atgggaatcg agatcaacgc cacccacagt
     1741 cgggcgatct ccgagggctg ggtgacggcg acctgcgacg ccctcgttct cggccgaacg
     1801 ctcgctaccc acgagatcgt gatcagagac gacgagggcc ggcggctctc gacggtgcgg
     1861 atgaccaaca tcctccgaga caagaactga tgtcgatccc tgccgactgg atcgagcacc
     1921 ggcggggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga gtcggagcta
     2041 cttcttcgcg ctgagctgct cgatgatggc ttgggccacg tcgtgcatcg tcaggcggcg
     2101 gtccatcgac gccttctgga tccagcggaa cgcttcgggc tcagtgaggc ccatcttctc
     2161 gttgagcagt cccttggcgc gatcgaccag cttgcgcgtc tcgaaacgct ccaccaggtc
     2221 ggcgacctcg gcctccagcg tgatgatctg cgtgtaccgg ctgagagcga tctcgatggc
     2281 gggcagcagg tcgttggggg tgaagggctt gacgacgtac gcgagtgcgc cagcctcgct
     2341 cgcgcgctca acgagctcct tctgactgaa ggcggtcagg agcacgactg gcgcgatgtg
     2401 gttcttgctg aggcgctcgg cggcggagat tccgtccagc tgcggcatct tcacgtccat
     2461 gatgacgagg tcgggacgca gctcagtagc cagcgccaca gcagtctcac catcggcggc
     2521 ctcgcccacc acctcgaagc cgttgtcgcg gagaatctcc acgatgtcca tgcggatgag
     2581 ggactcgtcc tcagcaacga cgactcgtcg tgggaccgtc gtagcggcgt cctggtctgt
     2641 cacgtgaatg agcctacggt attctcaaga tcgttgttgt gggccggatt ggcggaatgg
     2701 cagacgcagg gcactcaaaa tgctccgccc ggaagggtgt gcgggttcaa gtcccgcatc
     2761 cggcacgacc aagaggggca tcccgtacgg gatgcccctc ttctcgtcgt tgccgactca
     2821 gtcctgccag gcaggagcgg ctgcgttgat cgcgtcaccg atgatgtgca cgcgcatgtc
     2881 gttcgttgag cccgagatcc cgggagggga tccggagatg acgacgacct tgtcgccgat
     2941 ctcggcgaga ccctcacgca ggagcacctc gtcgacctgg cggtacatcg cgtcggtgtg
     3001 attcacacgc tccacgacga acgactcgat tccccagatc agcgccatgc ggcgacggat
     3061 ggcggggtcc ggagtgaacg ccttcatggg aacgcggaag cgcagccgtg acatccgtcg
     3121 ggcggcgtct ccggactcgg tgaacacgca cacgaacttc gcgtcgacga agtcggcgac
     3181 ttctgccgca gcgagcgtga ttgcgccacc ctgggtgcgg ggcttggtgc ccaacggagg
     3241 gatgcgctcg aggccgtgat cctcggtcga cgacacgatc cgggccatcg tctgcaccgt
     3301 gatgaccggg tactccccca cgctggtctc gcccgagagc atgaccgcgt cagctccgtc
     3361 gagaatggcg ttggcgacat ccgaggtctc tgcccgcgtc gggacggggc tcgagatcat
     3421 cgactccagc atctgcgtcg cgacgatcac gggcttcgcc atgcgccggg ccagctcgac
     3481 cgtgcgcttc tgcacaatcg gcaccgcctc gagcggaagt tcgacgccca gatcgccgcg
     3541 agcgaccatg atcgcatcga acgcgtcgat gatcgactcg aggtgatcga cggcctgagg
     3601 cttctcgatc ttggcgatga cgggaacctt gcgtccctcc tcggccatga tctcgtgcac
     3661 ccgcacgatg tcctcggcgt tgcgaacgaa tgagagagcg atgagatcgg cacccagctt
     3721 gaggccccag cgcaggtcgt cctcgtcctt ctccgacagc gcggggacgt tgacggccac
     3781 gcccgggagg ttgattccct tgttgttgga gactgcaccg gcgacgacca cctctgtcgt
     3841 cacgaccggg ccatcgacgc cgaccacgcg caccttcacc ttgccgtcgt cgatgagcag
     3901 gaagtcacct gcggcgacgt catcgggcag gcccttgaag gtggttccga cgatctcctt
     3961 ggtgccgagg atgtcctcgg tcgtgatctt gaagatgtca ccgaccgcga gctcgtgcgg
     4021 tccgttcgcg aacttgccga ggcggatctt gggaccctgg aggtcgacca tgacggcgac
     4081 tgcgcgaccc gaatcctctg ccgccttacg cacgttggcg tagacctcct cgtgcacggc
     4141 gtaggagccg tggctgaggt tcatgcgggc aacatcgaca cctgcatcga tgattgccct
     4201 gatgttctcg tagctcgacg ttgcggggcc gagtgttgcg acgatcttgg ctcgtctcat
     4261 gacttccttc tgtggtggta ctgctgtggt ggtgggagaa gactcgatga tcgagtccgt
     4321 ggagatcaga tggagatggc gtgatccgtg ggccgcacag ggaacggcag ctgggtatca
     4381 ccctcaaggt acttgtccac ggccgcggcc gcagcacgac cttcggcgat ggcccagacg
     4441 atcagcgact gcccgcgacc ggcatcgccg gcgacgaaga cgcccgactc gctggtctcg
     4501 tactggccgt cgcgctcgat ggtgcccttc tcgccgaacg gaaccgggag ctggtcctcg
     4561 agagtgcgag cctcggggcc cgtgaagccg agggccagaa gcaccagatc cgcgggaatc
     4621 tcgcgctcgg tgcccgactt cggcacgcgc cgtccatcca ggtactcggt ctcggcgacg
     4681 cgaatagcgc gcacctcgcc ggcctcgttc gcaaggaact cgacggtcga cgcgagatac
     4741 acccgctcac cgccctcctc gtgcgccgag gagacctcga acagagtggg ggccatcggc
     4801 cacggctggt gctccggccg ctcgctcggc ggctggatgc cgatggcgag gttggtgacg
     4861 gagaaggcgc cctgtcggtg ggccgtgccg atgcagtcag ccccggtgtc gccgccgccc
     4921 agcacgacga cgtgcttgcc ctccgccgtg atctggttgg cgacgacatc gcctgccccg
     4981 accttgttgg cctgcacgag gtactccatc gcgaagtgca cgcccatgag gtctcggccg
     5041 gggatgggca ggtcgcgtgg aacggtcgcc ccagttgcca cgatgacggc gtcgtagcgc
     5101 gacttcaggt catcccaggt gatgtcgacg ccgatctcca cgccggcacg gaaccgggtg
     5161 ccctcggcct gcatctgggc gagcctggac tcgaggtgct tcttctccat cttgaagtcg
     5221 ggaattccgt accgaaggag gccgccgatg cggtcgtcgc gctcgaacac ggcgacggtg
     5281 tggccggcgc gagtcagctg ctgcgcggca gccagtccag cagggcccga gccgacgacc
     5341 gcgacggtct tgccagtgag acgctcgggc gggtgcggcg tgacccagcc cttggcgaag
     5401 gcctcatcga tgatcgagac ctcgacctgc ttgatcgtga cggcgggctg gttgatgccc
     5461 agcacgcagg cactctcgca cggcgcgggg cacagccgac ccgtgaactc cgggaagttg
     5521 ttggtggcgt gcaagcgctc aatggcctga gccccctcgc ctcgccaggt caggtcgttc
     5581 cactcgggga tcaggtttcc gagcgggcag ccctggtgac agaacgggat gccgcagtcc
     5641 atgcaccgac ccgcctggcg gcggagctga gctgggtcct gctgctcgta gacctctttc
     5701 cagtccatga gtcgaagcga gacggggcgg cgcaccggag tctcgcgttc ctgcaccttg
     5761 aggaagccct tggggtcagc cacctgtcac ctccatgatg cgggaccaca cgacgtcgcc
     5821 atcggggtcg agtccttcgt tgatcgccgt ctcacgcgtc tgcagcacgg cggcgtagtc
     5881 gcgcggcagc accttggtga agcgcgcgac cgcggcatcc agctcgtcga gcatgcccgc
     5941 ggcgacggtg gaatcggtct cggcccggtg ccgctgcaac aggtcgcgca ggatctcgac
     6001 gtcggcactg cccaaaggca gtagctcgag ttcacccgag gagagtgact cggggttcac
     6061 ccgttccgtc ctgagatcgt agatgtaggc ggtgccaccg gacatgccgg caccgaggtt
     6121 gcgcccagtg ccgccgagga tcacggccag accgccggtc atgtactcga gagcgtggtc
     6181 gcccacaccc tcgacgaccg cagtagcacc ggagttgcgc accaggaagc gctcgcccac
     6241 gatgccgcgg atgaacatgc tgccctgggt agcgccgtaa ccgatgacgt tgcccgcgat
     6301 cacgttcttc tctgccttga acaggctcgc gcgatgcggg cggatgacga tctcgccacc
     6361 gcagagcccc ttgcccacgt agtcgttgct gtcgccctcg agacgcagcg tgataccgga
     6421 cggcatgaac gcgccgaagg actgacccgc ggtgcccgtg agtcgcacct cgatggttcc
     6481 cgtgggcagg ccgttctcgc catgccggag ggtgacctcg tggccgagca ttgttccgac
     6541 cgcacgctcc gtgttgcgga tcggtaggtc gatgacgacc ggcgtgccgt tgtcgagcgc
     6601 atccacgctc agctcgatga gttgacggtc gaagtgcttc tcgagctcgt gctcctggct
     6661 gacacggttg gtgcgcggct cgtcatccga gaactcgggt cccacgagga tcggcgacag
     6721 gtcgagcccg tcagccttcc agtgctgaac ggcacggtcg acatcgagca gttcgtggtg
     6781 gccgatcgcc tcctcgaggg tgcggaagcc cagctcggca aggtactcac gcacctcctg
     6841 agcgaggaac tcgaagaagt tgaccacgaa ctcgggctta cccgagaacc gggcgcgcaa
     6901 ctcggggttc tgggtggcga cgccgaccgg acaggtatcg aggtggcaga cgcgcatcag
     6961 gatgcagccc gacaccacta ggggcgcggt cgcgaaaccg tactcctccg cgccgagcag
     7021 ggcaccgatg atcacgtcgc gaccagtctt catctgcccg tcgacctgca cgaccacgcg
     7081 gtcgcgcatg ccgttgagca tgagcgtctg ctgggtctcg gccaggccga gctcccacgg
     7141 tgtgccggcg tgcttgagcg agttcaacgg gctcgcgcca gtgccgccat cgtgtcccga
     7201 gacgagcacg acgtcggcct tcgccttggt cacacccgcg gccacggcgc cgatgcccga
     7261 ctggctgacc agcttgacgt gcacacgggc atccgggttg gaccgcttga gatcgaagat
     7321 gagctgtttg aggtcttcga tcgagtagat gtcgtggtgc ggcggcggcg agatcaggcc
     7381 gacgcctgcg gtgccgtgac gtgtgcgggc gatccacggg tagaccttgc cagccggcag
     7441 ctggccgcct tcgccgggct tggcgccctg cgccatcttg atctggatgt ccgtggcgtg
     7501 cgtcaggtac atgctcgtga caccgaagcg accggatgcc acctgcttga tcgcactgcg
     7561 ccgctcgggg tcgagcaacc gggccacatc ctcgccaccc tcgccggtgt tggatcgggc
     7621 gccgagctgg ttcatggcga tcgcgagtgt ctcgtgcgcc tcctggctga tcgagccgta
     7681 gctcatcgcg ccggtgttga agcgcttgac gatcgactcg atcgactcga cctcatcgat
     7741 cggaaccgga cgccgctccc ccgaccggat acggaacagg ccgcgcaggg tcatgaggtt
     7801 ctcggcttga tcgtcgacga gcttcgtgta ctcgcggaag atgtcgtacc gtcgcgcgcg
     7861 agtcgcgtgc tgcagccgga agacggtgtc ggggttgaag aggtgcggag ggccttcgcg
     7921 gcgccactgg tactcgccgc cggtggcgag gcgctcgtga acgctcgtcg cgccatcctc
     7981 gggataagcc gatgagtggc gcgcggcact ttcggccgcg atcacctcca tgcccacgcc
     8041 gcccaggagg ctcgaggtgc cggtgaagta ctggtccacg aactcctgcg acagcccgac
     8101 ggcctcgaag gcctgagcac ccgcgtatga gccgacaaca gagatgccca tcttggacat
     8161 gatcttcagc acgcccttgc cgagcgcctt gatgatgttc ttgacggcct tctccggggt
     8221 gatcccctgg atcatcccgc tgcgcacgag ctcctcggcg gtctccatcg cgaggtaggg
     8281 gttgatggcg gatgcgccgt agcccacgag caacgcgacg tggtgcacct cacgcacatc
     8341 gccggcctcg acgatgaggc ccaccttcat gcggttctcc tcgcgaatga ggtggtggtg
     8401 cacggtcgcg agcatgagca gcgacgggat cggtgcgagg tccttgttgg aatcgcggtc
     8461 agagagcacg atgaattcgg caccgttggc gatcgcctcg tcgacctcct cgcacatctt
     8521 cgcgagacgc ttctccatcg ccttcgggcc cttgtcgaca cgatagagac cgcgaatcgt
     8581 ggtcgagagc tggcttccgg cgcgcgggtc gatgtgctgg atcttggcga gctc
//
LOCUS       DAOSOU010000076         8598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8173_length_8598_cov_5.363924, whole genome shotgun sequence.
ACCESSION   DAOSOU010000076 DAOSOU010000000
VERSION     DAOSOU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8173_length_8598_cov_5.363924"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLW15_05715"
     CDS             complement(<1..737)
                     /locus_tag="PLW15_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOT33931.1"
                     /translation="MSTLAPISLAELTAAADLQTRVDRKYVLTRAQADAVLAGLAPDT
                     RVLEIDGTRAMAYESVYFDTRDLLSYRMAAHARRRRFKLRTRSYVDSATSFLEMKTRG
                     ARSTTVKDRIEYDPAHRNVLTEEGREYAEAALDDLGIPEPERLDLVPTLVTRYRRRTL
                     YLPASGSRVTVDTDLSWESDDGTMIVRPDLAIIETKSGSRISGVDRLLWSHGHRPDRI
                     SKFGTGLAALRPDLPANKWARVLRRHFG"
     gene            complement(734..1315)
                     /locus_tag="PLW15_05720"
     CDS             complement(734..1315)
                     /locus_tag="PLW15_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOT33932.1"
                     /translation="MDQFLMLALDLVAISVLTFGLYFPRHRRRDLVVAFLVVNVGVAA
                     VSAVLGSTDVGLGLGLGLFGVLSIIRLRSSEIAQHEVAYYFAALSLGLIAGLTTTLSP
                     LMIALMGLILLVLFVGDNPAMFRRYRQQTIVLDSAYTDESALRDALERMLGAKVHRVA
                     VTNLDLVNDSTTVDVRYQLTDSVPSHESLGVGR"
     gene            1435..2907
                     /locus_tag="PLW15_05725"
     CDS             1435..2907
                     /locus_tag="PLW15_05725"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT33933.1"
                     /translation="MTPKSPEAPVRHVDVAVLGAGFAGIGMAARLAGRGIRDFVVIER
                     ADDVGGVWRDNTYPGAACDIRSDVYSLSFAPNPDWSRSYGSQPEILEYLRSTAREQGL
                     YEHMLFRTELEGASWDAAANLWRLSTSTGELTARVLVSGHGPLIDPVWPTIPGLESFA
                     GPRFHTAQWDHSVDLAGKRVAVIGTGASSIQVVPEVAKVAGHLTVFQRSAPWIIPRND
                     KPTSERRRRAFRRFPALQRIARRLEFLRAEVNFLGFKYKPVGAAAAQQSLAFLRKQVK
                     DPALRAKLTPDYRIGCKRILVTSAYYPALTRPNVSLETSAITAVEGSTIVTADGNRHE
                     VDVIIGGTGFNATEPSVARLVRGTDGRTLSEHWASHTAALRGTAVPGFPNLFLLNGPN
                     TILSHNSMIYIIESQVDYILQALDAAAGGTIEATPEALRAYNESLQRTMRTSVYLTGG
                     CSSYYLDAEGRNTVSWPHSAMAFRKAVRRLDAREFAVTPG"
     gene            complement(2894..5632)
                     /locus_tag="PLW15_05730"
     CDS             complement(2894..5632)
                     /locus_tag="PLW15_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOT33934.1"
                     /translation="MSVPTASYLESVDDVVASFETTSTGLTTAQADEHRARFGSNVIV
                     EVTHESGLRRYLRQYKDWMIVLLLASAVVTGILGDFGTAGVLVVLVVLNTLIGFVQEY
                     RAEKTMEALERLVDPLSEVYRDGELTQVASTLLVVGDVVRLTEGSSVPADVRLIRSTA
                     FSTNDFALTGESDPTRKFVQAIPHEVPVADRHNMAFAGTTVATGEAFAVVIATGMGTE
                     LGRIARLSQSAPVTRSPLQLETTKIARYVTYGVAVVTAIVLVIAVQSDLAIKDALLFA
                     VGFACALIPQGLPAEVNTALAAAAGILAKQNALVKRLSAVETLGATHVICTDKTGTLT
                     KNQMTVTELTVGGATYTSTGTGYDPAGTIAPTARGDAAARLTAFLSVGVLASNARLVP
                     PATDEPAWRILGDPTEGALVVLARKGGIDPEAVAAANEEIGELPFDSTRKLMTSIRRG
                     ADGTITAYAKGAPESILERATRIVDGTRVRPITAADREALLAVHTERATRALRNLLFA
                     TRTLTAKDAATNDLHVIEKDLTVLGMVSMIDPIRTAVPAAMADVLAAGVKVNIVTGDF
                     SLTAEAIARQAGLIGDDGITVVTGAELAGMSDEVVLEHASRGGTVFSRVAPEDKVRIV
                     DLVKAAGLVVAVTGDGINDAPALRHASIGVAMGATGTDVAKQAAEIVLLDDSFATLVT
                     AIRQGRTIYRNISKGVLSCLTSNAAEFVANSASLALASLGGVPLALNVLQILAIDLLG
                     EIFPIAALGRDRAEGETMKDPPRDPLRRILNKRSILDIFWAGALMGGFAIVNYLLFYG
                     RAGIDPFTEAVPADVLAPATTMTYVTIMVCQLVSIVQRRSVHGFFTRYQFTNRTFWLA
                     IGVAVAIMLVIVYVPFVAGFFGTGAISLLDWGFVLLAAVVFLALRELQRVLGLSRV"
     gene            complement(5681..6010)
                     /locus_tag="PLW15_05735"
     CDS             complement(5681..6010)
                     /locus_tag="PLW15_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HOT33935.1"
                     /translation="MEIATDLLVEVHIPLTPTPDLDPEDQFDWIDTVLDFLEELEDSG
                     EVALYDDGESLDGIYVFSIHSATQAELLAIARRVADLPGVPTGVFAVVTDIEAENYGE
                     GERFDIS"
     gene            complement(6073..6684)
                     /locus_tag="PLW15_05740"
     CDS             complement(6073..6684)
                     /locus_tag="PLW15_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33936.1"
                     /translation="MRVFPQRWWYLGVGAVLGLMIGAGLAARITDSDTGSTLGGGGLG
                     AIAGILFGVAVITAALPHDARLFRLRRRFPDAVVLQALRDEDNAAVLHDVAKVPAATR
                     FSVVFDGTGASVWDSADAAEPLVAFPWAQIGAITVGGMVRPTRLGTRAFFRLVVALLV
                     RGETVALPFGVERVPAFPGGRTTLSDGELESLAQRLTEVRPTT"
     gene            complement(6733..7536)
                     /locus_tag="PLW15_05745"
     CDS             complement(6733..7536)
                     /locus_tag="PLW15_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33937.1"
                     /translation="MPERVEAWWARRQWSKGSAVPYAIGEYRHEWARWPVLVRQYHPD
                     LNSGVVLSQVPPAADVWLQWQCEVGHVFVATPSEVRSRAGVSRRRSSWCPDCFAGAAP
                     PKARPSRAVRLCAKSDAPAHPIGEPFVSACAPTAASAAEPRLVQLLSSRLAYSDGFNA
                     VRVPRAFFTHLEVWPDIILPELRVAIEYDTMGRNSTEHVGQREVLDKRKDRLLRSAGW
                     EVVRVRTGKLRPIGEHDVAASGVTGKLVDRLLDELRVIRGDLIVDCYLR"
     gene            complement(7567..8118)
                     /locus_tag="PLW15_05750"
     CDS             complement(7567..8118)
                     /locus_tag="PLW15_05750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT33938.1"
                     /translation="MTLLDTVTPPTSTAAERADRLTAAGAAWGARIAADTAAAQLTYK
                     VRGRGEGSVASLIKAGKHSFLVDEPAALAGDDVAASPVEFALGALISCQIVVYRLYAQ
                     ALGIQVDDISIDAEGDLDARELFGIEGGARAGFTHVRLAITITGPETQERYTELRDVV
                     DQHCPVLDLFVNATPVSATVTKG"
     gene            complement(8270..>8598)
                     /locus_tag="PLW15_05755"
     CDS             complement(8270..>8598)
                     /locus_tag="PLW15_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33939.1"
                     /translation="ETADDPTDQPGDDTPGTTPTAVPEDEDGAGDESTEGDGAGKPAD
                     TPGNGGEGTGKPEDTPGNGGDGTGKPEGTPGNGGDGTGKPADTPGNGGDGTGKPDNTP
                     GGNPNK"
BASE COUNT         1397 a         2870 c         2995 g         1336 t
ORIGIN      
        1 ccgaagtggc ggcgcagcac ccgtgcccac ttgttggcag gaaggtcggg gcgcaaggcg
       61 gcaaggcccg tgccgaactt ggagatgcgg tcggggcggt ggccgtgcga ccacagcagc
      121 cgatcgacac cggagatgcg cgatcccgac ttcgtttcga tgatcgcaag gtcgggccgc
      181 acgatcatgg tgccgtcgtc ggactcccag gagagatccg tgtcgacggt aacccgactg
      241 cccgacgcgg gcaggtagag cgtgcggcgc cggtagcggg tcaccagcgt cggcaccagg
      301 tcgagccgct caggctcggg gatgccaagg tcgtcgaggg cggcctccgc gtactcgcga
      361 ccctcctcgg tgagcacgtt gcggtgcgcg gggtcgtact cgatgcggtc cttcaccgtc
      421 gtcgagcgcg caccccgggt cttcatctcc agaaaggatg tcgccgagtc gacgtagctc
      481 cgcgtgcgca gcttgaaccg ccgacgccgt gcgtgtgctg ccatgcggta gctcagcagg
      541 tcgcgggtat cgaagtagac ggactcgtag gccatcgccc gcgtgccgtc gatctcgagc
      601 acgcgcgtgt cgggcgccag gccggccagc accgcgtcgg cctgtgcccg ggtgagcacg
      661 tacttgcggt cgacacgggt ctggaggtct gcggcggcgg tgagctcggc gagggagatc
      721 ggtgcgagtg tgctcatcgc cccactccca gcgactcgtg gctcggcacc gaatcggtga
      781 gctggtagcg cacgtcgacg gtggtggagt cgttcacgag gtcgaggttc gtgaccgcca
      841 cccggtgaac cttggccccg agcatccgct cgagcgcatc gcgcagtgcg gactcgtcgg
      901 tgtacgcgga gtcgagcacg atcgtctgct gccggtagcg acggaacatc gcggggttgt
      961 cgccgacgaa cagcacgaga aggatcagcc ccatcaacgc gatcatgagc gggctgaggg
     1021 tcgtggtgag tccggcgatc agacccagcg acagcgcggc gaagtagtac gccacctcgt
     1081 gctgggcgat ctccgaactg cgcagccgga tgatcgagag cacaccgaac aggccgagcc
     1141 cgagtcccag ccccacgtcg gtcgagccga gcacagccga gacggcagca acgcccacgt
     1201 tgacgacgag gaaggcgacc acgaggtcgc gacgccggtg acggggaaag tagaggccga
     1261 aggtgagcac tgagatggcc acgaggtcga gggcgagcat gaggaactga tccatgggat
     1321 gactcctggg cagatgaacg gcaggtgaac gagttacatt cagccgccgg tgcctatgtg
     1381 cttgctatga gacgcgtggg aagcagatac ggagtttgtg gttcactgat tgccatgacg
     1441 ccgaagtctc ccgaagcccc tgtccgccac gtcgatgttg ctgtgctggg ggcggggttc
     1501 gccggtatcg ggatggcggc gcggttggct ggccggggca tccgggactt cgtggtcatc
     1561 gagcgcgctg acgacgtcgg cggggtctgg cgtgacaaca cctacccggg tgccgcgtgc
     1621 gacatccgca gcgacgtcta ctcgctgtcg ttcgcgccca acccggactg gtcgcgcagc
     1681 tacggctccc aacccgagat tctcgagtac ctgcgctcga ccgcccgcga gcagggactc
     1741 tacgagcaca tgctgttccg caccgagctc gagggggcca gctgggatgc cgcggcgaac
     1801 ctctggcgcc tgtccacgag caccggcgag ctcaccgccc gcgtgctcgt ctcgggacac
     1861 ggtccgctca tcgacccggt ctggccgacg atccccggcc tggagtcgtt cgctggtccg
     1921 cgcttccaca cggcgcagtg ggaccactcg gtcgacctcg cgggcaagcg cgtcgccgtg
     1981 atcggcaccg gggcatcctc gatccaggtg gtgccggagg tggcgaaggt cgcggggcac
     2041 ctgacggtgt tccagcgctc tgcgccgtgg atcatccccc gcaacgacaa gccgacctcc
     2101 gagcgtcgac gccgcgcgtt ccggcgcttc cccgcgctgc agcgaatcgc ccgacggctc
     2161 gagttcctcc gggccgaggt gaacttcctc ggcttcaagt acaagccggt cggtgcggcc
     2221 gccgcccagc agtcgctcgc cttcctgcgc aagcaggtga aggatcccgc gctacgcgcc
     2281 aagctcacgc ccgactaccg catcggctgc aagcgcatcc tcgtgaccag cgcctactac
     2341 cctgcgctga cccgccccaa cgtgagcctt gagacgagcg ccatcacggc ggtcgagggc
     2401 tccactatcg tcacggccga cggcaaccgt cacgaggtcg atgtcatcat cggcggcacg
     2461 ggcttcaacg cgaccgagcc gtcggtcgct cgtctggtgc gcggcaccga cggccgcaca
     2521 ctctccgagc actgggcctc gcacacggcg gcactgcgcg gcaccgcggt cccgggcttc
     2581 cccaacctgt tcctgctcaa cggaccgaac acgatcctga gccacaacag catgatctac
     2641 atcatcgagt cgcaggtgga ctacatcctc caggcactgg atgccgcggc cggcggcacc
     2701 atcgaggcca cccccgaagc tctgcgcgcc tacaacgagt ccctccagcg cacgatgcgc
     2761 acgtcggtct acctgaccgg cggatgctcc agctactacc tcgacgccga gggccgcaac
     2821 acggtcagct ggccgcactc ggcgatggcg ttccgcaagg ccgtccgcag gctcgacgcg
     2881 cgcgagttcg cggtcacacc cggctgagcc ccagcacccg ctgaagttcg cgcagggcga
     2941 ggaacaccac ggcggccagc agcacgaatc cccagtcgag caggctgatc gcaccagtgc
     3001 cgaagaagcc agccacgaac ggcacgtaca cgatcaccag catgatcgcg acggcgacgc
     3061 cgatggccag ccagaatgtg cggttcgtga actggtagcg ggtgaagaat ccgtgcacgc
     3121 tccgccgctg cacgatgctc acgagctgac agaccatgat cgtgacgtag gtcatggtgg
     3181 tcgccggcgc gagcacgtcg gcgggcacgg cttcggtgaa cgggtcgatg ccggcacgtc
     3241 cgtagaagag caggtagttg acgatggcga acccgcccat gagcgcgccc gcccagaaga
     3301 tgtcgaggat cgaccgcttg ttcaggatgc gccgcagcgg gtcacgagga gggtccttca
     3361 tggtctcccc ctcggcccga tcccgcccga gcgctgcgat cgggaagatc tcgcccagca
     3421 ggtcgatcgc gaggatctgc agcacgttga gggcgagcgg cactcccccg agggatgcca
     3481 gggcgaggct ggccgagttg gccacgaatt cggccgcgtt cgaggtgagg cagctgagca
     3541 cacctttgct gatgttgcgg tagatcgtgc gaccctgacg gatcgcggtg acaagggtcg
     3601 cgaagctgtc gtcgagcagc acgatctcgg cggcctgctt ggcgacgtcg gtgccggtcg
     3661 cgcccatcgc gacgccgatg ctggcgtggc gcagtgctgg ggcgtcgttg atgccatcgc
     3721 ccgtgacggc cacgacgaga cccgcggcct tgacgaggtc gacgatgcgc accttgtcct
     3781 ccggggcgac gcggctgaag accgtgcccc cgcgggacgc gtgctccaga acgacctcgt
     3841 cggacatccc ggcgagctcg gcgcccgtga cgacagtgat gccgtcgtca ccgatgagtc
     3901 cggcctggcg ggctatggcc tccgcggtga gcgagaagtc gccggtcacg atgttgactt
     3961 tcacccccgc ggcgagcaca tcggccatcg ctgcgggcac cgccgtgcgg atcgggtcga
     4021 tcatcgagac catccccagc accgtgagat ccttctcgat gacgtgcaga tcgttggtgg
     4081 ctgcgtcctt ggccgtgagc gtgcgggtgg cgaaaagcaa gttgcgcagc gcgcgcgtcg
     4141 ctcgttcggt gtgcaccgcg agcagggcct cccggtcggc ggcggtgatg ggtcgcaccc
     4201 gggttccgtc gacgatgcgc gtggcccgct cgaggatgct ctcgggagcg cccttcgcgt
     4261 aggcggtgat cgtgccatcc gcgccgcgac gaatcgaggt catgagcttg cgggtggagt
     4321 cgaacggcaa ctcgccaatc tcctcattgg cggcggcgac agcctccggg tcgatgccgc
     4381 ccttgcgggc gagcaccacc agagcgccct ccgtggggtc gccgaggatg cgccacgcgg
     4441 gctcgtcggt cgcgggcggc acgagccgcg cgttgctggc gagcactccg acgctgagga
     4501 acgcggtgag gcgagccgcg gcatcccctc gtgcggtcgg ggcgatggtg cccgccgggt
     4561 catagccggt gcccgtggac gtgtaggtcg cacccccgac ggtcaactcg gtgacggtca
     4621 tctggttctt ggtgagcgtg cccgtcttgt cggtgcagat cacatgggtg gcccccaggg
     4681 tctcgacggc gctgaggcgc ttgaccaggg cgttctgttt ggcgaggatg cccgctgcgg
     4741 ccgcgagggc ggtgttcacc tcggcgggca gaccctgcgg gatcagagcg cacgcgaacc
     4801 cgacggcaaa gagcagtgca tccttgatcg cgaggtcgga ctgcacggcg atcacgagca
     4861 cgatcgccgt gaccacagcc acgccgtagg tcacgtagcg cgcgatcttg gtggtctcga
     4921 gctgcagcgg gctgcgggtc accggggcgg actggctgag tctggcgatg cgacccagct
     4981 cggtgcccat gcccgtcgcg atcacgacag cgaacgcctc gccggtcgcc acggtcgtgc
     5041 ctgcgaaggc catgttgtgc cggtcggcga ccggtacctc gtgcgggatg gcctgcacga
     5101 acttgcgggt cggatcgctc tcgccggtga gcgcgaagtc gttcgtcgag aacgccgttg
     5161 accggatcag gcgcacgtcg gcgggcaccg acgaaccctc ggtgagccgc accacatcgc
     5221 ccaccacgag gagggtggag gcgacctggg tcagttcccc gtcacggtag acctcgctga
     5281 gcgggtcgac gagccgctcg agtgcttcca tcgtcttctc cgcgcggtac tcctgcacga
     5341 agccgatgag cgtattgagc acgacgagca ccacaagcac gcctgcggtg ccgaaatccc
     5401 cgaggatgcc cgtgacgacc gcgctcgcca gcaggagcac gatcatccag tccttgtact
     5461 ggcgcaagta gcgccgcaga ccggattcgt gcgtgacctc cacgatcacg ttcgaaccga
     5521 aacgggctcg gtgctcgtcg gcctgtgcgg tcgtcagccc ggtcgaggtg gtctcgaagg
     5581 aggcgacgac atcgtccacc gattcgaggt acgacgcggt cggcacgctc atcgtgacga
     5641 ccggagcgaa cctgatcgtg gtgagctcat agtggtgagc ttacgagatg tcgaagcgct
     5701 cgccttcgcc gtagttctcg gcctcgatgt cggtgaccac ggcgaacaca ccggtgggca
     5761 cgccgggcag gtctgcgacc cgtcgggcga tcgccaggag ttcggcttgg gtcgcgctgt
     5821 ggatcgagaa cacgtagatg ccgtcgagcg actcgccgtc gtcgtacagg gccacctcgc
     5881 cggagtcctc gagttcttcg aggaagtcga gcaccgtgtc gatccagtcg aactgatcct
     5941 cggggtcgag gtcgggagtg ggcgtgaggg gaatgtgcac ctcgacgagc aggtcagtcg
     6001 cgatctccat tgcaccagtg tgctcgcggg cctcgagacc gaaggtgaat tcttggcgaa
     6061 ctgtgccggg gctcaggtgg tcgggcgaac ctcggtgagt cgctgagcga gcgactccaa
     6121 ctccccgtcg ctcagagtcg tgcgcccgcc ggggaatgcc gggacgcgct ccactccgaa
     6181 cggaagtgcg acagtctcac cccgcacgag gagagcgacg accagtcgga agaaggcacg
     6241 agtaccgagc cgggtgggtc gcaccatgcc gcccacggtg atggcgccaa tctgggccca
     6301 agggaaggcg acgagcggct ctgccgcatc cgccgaatcc cagacactgg ctcctgtgcc
     6361 gtcgaacacg acggagaatc gagtcgcagc gggcaccttc gccacgtcgt ggagaacggc
     6421 ggcgttgtcc tcgtccctca gcgcctgaag taccaccgcg tcgggaaacc gtcgccggag
     6481 ccggaacagg cgtgcgtcgt gaggaagagc cgcggtgatc accgcaaccc cgaagagaat
     6541 gccggcgatc gcgccgagac ctccgccccc cagagtactg ccggtgtcgg agtcggtgat
     6601 tcgcgccgcc agccccgcac cgatcatcag tccgagaacc gcgccgacac cgaggtacca
     6661 ccatcgctgc gggaacactc tcaccactga cctccactcg acgcgcacca ccgcgcgggc
     6721 gcgtgccaga cactaccgca gatagcagtc cacgatcagg tcgccgcgga tcacccgcag
     6781 ctcatcgagc aaccgatcga caagcttgcc cgtcaccccg gatgccgcga catcatgctc
     6841 cccgatgggc cgaagtttgc ccgtcctgac ccgcaccacc tcccagcccg ccgagcgcag
     6901 caaccggtct ttgcgtttgt cgagaacctc gcgctgaccc acatgctcgg tggaattgcg
     6961 acccatggtg tcgtactcga tcgcgaccct cagctcgggc aggatgatgt cgggccagac
     7021 ctccaggtgg gtgaagaacg cgcgcggtac gcgcacggcg ttgaaaccgt ccgagtaggc
     7081 caggcgtgag ctgagcagct ggaccaggcg cggctcggcc gcggacgcgg ctgtgggggc
     7141 gcacgcgctt acgaagggct cgccgatggg gtgggccggg gcatccgact tcgcgcacag
     7201 acgcacggcc ctcgaggggc gggctttcgg cggggccgcg cccgcgaagc agtcggggca
     7261 ccacgacgac cggcgccgcg agacgccagc acgcgagcgc acctccgacg gggtggcgac
     7321 gaacacgtgg ccgacctcgc actgccactg cagccagaca tccgctgccg ggggcacctg
     7381 agagagcacg actcccgagt tgaggtcggg atgatactgc cgcaccagca cgggccagcg
     7441 ggcccactcg tgccggtact ccccgatcgc gtagggcacg gcggaaccct tggaccactg
     7501 gcgtctcgcc caccacgcct cgacccgctc cggcatgcag tgatgctaga gcgggccgac
     7561 gacgttctag cccttggtga cggttgcgct caccggggtc gcgttgacga acaggtcgag
     7621 caccgggcag tgctggtcga ccacgtcgcg cagctcggtg tagcgctcct gggtctcggg
     7681 gccggtgatg gtgatggcca ggcgcacgtg ggtgaacccg gcacgcgcgc cgccctcgat
     7741 gccgaagagt tcgcgggcgt cgaggtcgcc ctcggcgtcg atggagatgt cgtcgacctg
     7801 gatgccgagt gcctgcgcgt agaggcggta gaccacgatc tggcaggaga tgagagcacc
     7861 gagcgcgaac tcgacggggc tcgccgccac gtcgtcgccg gcgagcgcag caggctcgtc
     7921 gaccaggaag ctgtgcttgc cggccttgat gagcgaggcg acgctaccct cgccacggcc
     7981 acggaccttg taggtgagct gtgcggcggc ggtgtcagcg gcgatgcggg caccccaagc
     8041 tgcgcctgcg gcggtgagtc ggtccgcgcg ctcagcggcg gtcgacgtgg gcggggtcac
     8101 ggtgtcgaga agtgtcatga gggcaccgta gggcggatgc cgcggcatcc gtccgatgtg
     8161 acgtcacgca ccgacaccga acgtaaccgg gcgtctcaca acgtcatgaa aggtgccgag
     8221 tggtccccca cttcccgtaa gcgagggacc actcgaactc gtggggcggc tacttgttgg
     8281 ggttgccgcc aggcgtgttg tccggtttgc cggtgccgtc gccaccgttg ccgggggtgt
     8341 cagccggctt gccggtgccg tcgccgccgt tgcccggcgt gccttcgggc ttgccggtgc
     8401 cgtcgcctcc attgccgggt gtgtcctccg gcttgccggt accctcaccg ccgttaccgg
     8461 gggtgtcggc cggcttgccg gcgccatcgc cctctgtcga ttcatcgccc gcaccgtcct
     8521 catcctcggg aacggcggtc ggggtggtgc cgggggtgtc gtcgcccggc tggtcggtcg
     8581 ggtcatccgc tgtctcgc
//
LOCUS       DAOSOU010000077         8510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8266_length_8510_cov_4.892135, whole genome shotgun sequence.
ACCESSION   DAOSOU010000077 DAOSOU010000000
VERSION     DAOSOU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8266_length_8510_cov_4.892135"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLW15_05760"
     CDS             <1..350
                     /locus_tag="PLW15_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT33940.1"
                     /translation="KDALGRRGEEIAARYLASRGLAIVERNWRCPQGEIDLIARDGGE
                     LVFVEVKTRSSVAYGHPLESITPVKLARLRRLAAAWCDAHPGGHDTIRIDAIAVIAPR
                     VGDVEVEHLERVF"
     gene            347..1882
                     /locus_tag="PLW15_05765"
     CDS             347..1882
                     /locus_tag="PLW15_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT33941.1"
                     /translation="MMLGRTYAVSLLGLTGEIVEIEADLSSNLPAFVLIGLPDAALGE
                     AKDRVRAAAINSGCGLPNRKITVNLSPAALPKHGSGFDLAIALAALAAAGTVSAESIG
                     GVLHLGELGLDGRLRPINGVLPAVLAASRAGFESVMVPTGNADEAALVPGVRVIPVAS
                     LREAAIWHGGEFEPEVVEPILRASADDPDDDEGDLGDVIGNPDAVEAMIVAAAGGHHV
                     FLLGPPGAGKTMLAARLPGLLPDLSARAALEVSSIRSLAGLSVGRSLVRRPPFEAPHH
                     TASAAALVGGGSGQIRPGAAARASDGVLFLDEAPEFSPSVLDALRQPLESGLITIHRA
                     NAVATFPGRFQLVMAANPCPCGQYGARDSECTCPPAARRRYVGRLSGPLLDRIDIQLR
                     VQRITAAQLRLAHETRGVSSAIARERVSVARAAAADRLADTPWQLNSQVPGAWLRGPG
                     VRLSPAVTASIDRALERGGITMRGYDRVLRLAWTIADLAGDTSPNAAQLGQALYLRRA
                     MSN"
     gene            1885..3096
                     /gene="dprA"
                     /locus_tag="PLW15_05770"
     CDS             1885..3096
                     /gene="dprA"
                     /locus_tag="PLW15_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT33942.1"
                     /translation="MFGLKEAEVAELIRAVAGRDLDRDAIEERFARATWSGIAEPGDG
                     VAGLVIDAIGAPRALAAVVERWSAERLLAELGANGAATEGDLQQALDRWMPRLNSGAA
                     LIALRQASRVGAALRIPGDELWPVGLDDLQIHAPLALWTRGTTTALRALTRSIALVGA
                     RAATGYGEHVTMESSAGLVDRGYAIVSGAAYGIDGMAHRAALASKGHTVAFLAGGVDR
                     FYPSGHDALLTRIVESGVVVSELPPGSPPTKWRFLQRNRLIAAASLATVVVEAGWRSG
                     SLNTAHHAGSLGRPVGAVPGPVTSAASSGCHRLMREGAAICVTTADEMAELAPLDALT
                     AASPRAGGFAEDTRLLDAMSTRAPRVASDIAARAGLSIAEVQALLGQLELTGAVGERE
                     RGWLKVERKTE"
     gene            3132..3755
                     /locus_tag="PLW15_05775"
     CDS             3132..3755
                     /locus_tag="PLW15_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOT33943.1"
                     /translation="MGALVRRPALISAALAPVFFIGGTIVAGALWPEYNPVTQTISEL
                     AAGDAPTRVFMTVAFMLTAVSHGVTGIFATGVGWPGRVALMAASVATFAVALFPLPTV
                     AGTSVEHRWSAIAGFILLAIWPVLGMRRSTGVPERYPWIIRPVGAILGTGLMAVFCFW
                     FLAVWASPGLGYIGVIERVAANVESVWPLVVVLGLWLHERPGRRLAE"
     gene            3752..4681
                     /locus_tag="PLW15_05780"
     CDS             3752..4681
                     /locus_tag="PLW15_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOT33944.1"
                     /translation="MIQLGQYPAPSHTIAHFSDTHLLAGGRPLYGKVNTIDHLAQALA
                     QLERSIAKPEAIVFTGDLADLGEPDAYVRLRELVEPAAARMNARVIWVMGNHDERGDY
                     SRLLFDEESDAPQDRVYDINGLRIISFDTTVPGYHHGEVTDDQLDWLADVLSSPAPHG
                     TLLAVHHPPIPTPLLEAMGMLELFDQHKLAAVLEGSDVRGILAGHLHYSTHSTFAGIP
                     VSVASATCYTLDLSAEDRLLSGVDFGQSVNIVHVYDKQLVHSIVPIGATTEVSGFPAG
                     AWAQIEAMTPEERIETFSKKSSTFNSAEVSSNG"
     gene            4819..>5288
                     /locus_tag="PLW15_05785"
     CDS             4819..>5288
                     /locus_tag="PLW15_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOT33945.1"
                     /translation="MDLTRAADDFASYLAADRGFSPHTVRSYRSDLSQLVDFATERGA
                     AESQDLDLELLRDWLWRSSQAGLAKSSLARRAAAARSFTAWLARTKASPADAATRLRA
                     PKSDHHLPRVLTREQIGGILDRVAERADTGDPVAIRDRAIIELLYASALRVSELT"
     assembly_gap    5289..5388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5522..7597
                     /locus_tag="PLW15_05790"
     CDS             5522..7597
                     /locus_tag="PLW15_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT33946.1"
                     /translation="MHRLTSFITGRRTSWIVLVTFLLAAGAVFAVGSGATSDTSPGIG
                     LPASAESARVAAAQEDLPSADSTSALLVFSRDGAKLTDADIEAVTAAASDLAGLSKDD
                     FVPPPTVSDDGTAALVVVPLEIIEDVAAQADRAEELRTTANADLPSGLTALLTGAEGF
                     AVDVAAVFKGADFTLLLTTVIVVAVLLLITYRSPWLWLVPLVVVGTADGLASIAATRV
                     AALAGITLDASVTGILSVLVFGAGTNYALLLIARYRDELRLHEDRRDAMARALRGAGP
                     AIIASGSTVTLALLTLVFAQLEGNRALGIACATGVVIAMLFALFVLPAALVLFGRGLF
                     WPYVPRFGSEGSMDRGVWHRLGVLVSKKPVVVAIVGAVILGSLALGTTQIKVGLSQTE
                     RFTSVPEAVVGQNIIADAFSAGSGSPAAVITNADYADKVASVAETVDGVDSATVGETN
                     GTITQVNVVLDAAAETPESFAAIEALRAAMHDIDGADAIVGGLDAQSLDVTRAQTADQ
                     DLVIPLILVLVFLVLVLLLRALVAPVLLLATVVASFFASLGASWLLFQSVFGFPAIDT
                     NVVLFSFLFLVALGVDYNIFLVTRAREETREFGTRTGMIRALSSTGGVITSAGILLAA
                     VFAVLGVLPLITLTQIGIIVCIGVLLDTLLVRTVIVPSLAFITGEKFWWPGRPMTVAA
                     EVRTTGSGS"
     gene            complement(7705..8124)
                     /locus_tag="PLW15_05795"
                     /pseudo
     CDS             complement(7705..8124)
                     /locus_tag="PLW15_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
BASE COUNT         1203 a         2973 c         2874 g         1360 t
ORIGIN      
        1 cgaaggacgc gctcgggcgg cggggtgagg agatcgcggc gcgctatctc gcctcccgcg
       61 ggttggcgat cgtcgagcgc aactggcggt gcccccaggg cgagatcgac ctcatcgcac
      121 gcgacggggg cgaactcgtg ttcgtggagg tcaagacacg gtcgagcgtg gcgtacggtc
      181 atccgctcga atcgatcacg cccgtcaagc tcgcgcgact gcggcggctc gcggcggcgt
      241 ggtgcgatgc gcatccgggc gggcacgaca cgatccgcat cgacgcgatc gcggtcatcg
      301 cgccacgcgt cggcgacgtc gaggtcgagc acctcgagcg ggtcttctga tgctggggcg
      361 cacctacgcc gtgtccctcc tgggtctcac cggcgagata gtggagatcg aggccgacct
      421 gtcgagcaac ctgccggcgt tcgtgctgat aggccttccg gatgcggcgc tcggcgaggc
      481 caaagatcgt gtgcgcgccg ccgcgatcaa ctccggctgc ggcctgccca atcgcaagat
      541 cacggtcaac ctctcgcccg ccgcgctgcc caagcacggc tcgggattcg acctcgcgat
      601 cgcgctcgcc gcgctcgcgg cggcgggcac cgtcagcgca gagtcgatcg ggggagtcct
      661 gcacctggga gagctcggtc tcgacgggcg gctccgaccg atcaacggtg tgcttcccgc
      721 agtgctcgcg gcctcgaggg cggggttcga gagcgtgatg gttccgaccg gcaatgcgga
      781 tgaagcggcg ctcgtgcccg gtgtgcgcgt catcccggtc gcatcgttgc gcgaggcagc
      841 gatctggcac ggcggggagt tcgagcccga ggtcgtcgag cccatcctcc gggcttcggc
      901 ggacgacccg gatgacgacg agggtgacct cggcgacgtg atcggcaacc ctgatgccgt
      961 cgaggcgatg atcgtcgcgg cggcaggtgg acaccacgtc ttcctgctcg ggccgcctgg
     1021 tgcaggcaag acgatgctcg ccgcgcgcct gcctgggctc ttgcccgacc tcagcgctcg
     1081 cgccgcgctg gaggtcagtt cgatcagatc cctcgcaggc ttgtcggttg gcagatcgct
     1141 cgttcgccgc ccgccgttcg aggcacccca ccacaccgcc agcgccgcag ccctggtcgg
     1201 tggtggcagc gggcagatcc gcccgggtgc ggcggcccga gcatccgacg gagtgctctt
     1261 cctcgatgag gcgcccgagt tcagcccgtc ggtactcgac gcgctgcgcc agccgctgga
     1321 gtctggactc atcacgatcc accgggcgaa tgccgtggcg accttccccg ggcggttcca
     1381 gctggtgatg gcggcgaacc cgtgcccgtg cgggcagtac ggggcacggg attccgagtg
     1441 cacgtgccca cccgctgcgc gccgacggta cgtcgggcga ctgtcgggtc cgttgctgga
     1501 tcgcatcgac atccagctgc gggttcagcg cattacggct gcgcaactgc gcctcgccca
     1561 cgagactcgt ggcgtatcgt cggcgatcgc gcgcgagcgg gtctcggtcg cgcgcgcggc
     1621 ggccgccgat cggctggcgg acacgccttg gcagttgaat tcgcaggtgc cgggggcgtg
     1681 gttgcggggg ccgggggtgc ggctgtcacc ggcggtgacg gcatccatcg atcgcgccct
     1741 cgagcgcggc ggaatcacca tgcgcggcta cgaccgcgtg ctgcggctgg cctggaccat
     1801 cgcggatctc gcgggcgaca ccagcccgaa cgccgcccaa ctggggcagg cgctgtatct
     1861 gaggagggcg atgtccaatt gagcatgttc ggattgaaag aagccgaggt cgccgaactg
     1921 atccgcgcgg tcgcgggtcg tgacctggac cgggacgcga tcgaggagcg gttcgcccgc
     1981 gccacctggt cgggtatcgc tgagcccggc gacggggttg cagggttggt gatcgatgcc
     2041 atcggcgcac cacgagcgct cgccgccgtt gtcgaacgat ggtcggccga acgactgctc
     2101 gccgagttgg gagcaaacgg ggcagcgacc gagggcgacc tgcagcaggc cctcgaccgc
     2161 tggatgccgc ggctcaactc cggcgcggcc ctcatcgccc tgcggcaggc ctcgcgggtg
     2221 ggagccgcat tgcgcatccc cggggacgaa ctctggcccg tcggcctcga cgacctgcag
     2281 atccacgcgc cgctcgccct ctggacgcgc ggtaccacga cggcgcttcg tgccctcacg
     2341 aggtcgatcg cccttgtcgg tgcgcgcgcc gcgaccggct acggggagca tgtcacgatg
     2401 gagagttcgg ccggtctcgt cgaccggggg tacgcgatcg tctctggcgc cgcctacggc
     2461 atcgacggga tggcccaccg ggccgcgctc gccagcaagg gccacaccgt cgcctttctg
     2521 gcgggagggg tcgatcgctt ctacccctcg ggtcacgacg ccctgctgac ccgaatcgtc
     2581 gagtcgggcg tcgtggtctc ggaactgccg cccggttcac cgccgaccaa gtggcggttc
     2641 ctccagcgca atcgcctcat cgccgccgca agtctcgcga cggtagtggt cgaggcgggc
     2701 tggcgctcgg gttcgctcaa caccgcgcac catgctggtt ccctcggaag gccggtcggg
     2761 gcggtgcccg ggcccgtaac cagtgcggcc tcatccggat gccaccgcct gatgcgggag
     2821 ggcgcagcga tctgcgtcac aacggctgac gagatggccg agctggcgcc gctcgacgcg
     2881 ctcacggcgg cgtcgcctcg ggccggcggc ttcgccgagg acacccgtct gctcgacgcg
     2941 atgagtacgc gcgcacccag ggttgcatcc gacatcgcgg cccgggcggg cctgtccatc
     3001 gccgaggtgc aggcgctcct gggtcagctg gagctgaccg gcgcggtggg ggagcgggaa
     3061 cgggggtggc tcaaagtcga gcgcaaaacc gagtgagcgg cgcgtggcat ccgtcgcgct
     3121 agccttgccg catgggtgca ctcgtccgtc gtccggcgct gatctcggcg gccctcgcgc
     3181 cggtgttctt catcggcgga accatcgtcg ccggcgccct gtggcccgag tacaacccgg
     3241 tcacccagac catcagtgag ctcgccgcgg gggatgcccc cacccgcgtg ttcatgaccg
     3301 ttgcgttcat gctcaccgcc gtcagtcacg gcgtgacggg catcttcgcg accggggtcg
     3361 gctggccggg ccgcgtcgcg ctcatggccg catccgtcgc caccttcgcg gtcgcgctgt
     3421 tcccactgcc gacggtggcg ggcacgagcg tcgagcaccg ctggagtgcg atcgcgggct
     3481 tcatcctgct ggccatctgg cctgtgctcg gcatgcgccg cagcaccggg gtgcccgagc
     3541 gctacccgtg gatcatccgg ccggtcggcg ccattctcgg caccgggctc atggcggtgt
     3601 tctgcttctg gttcctcgcg gtgtgggcca gccctggtct cggctacatc ggcgtgatcg
     3661 agcgggtcgc cgcgaacgtc gagagcgtct ggccgctcgt tgtcgtgctc gggctctggc
     3721 tgcacgagcg acccggccgt aggctggccg agtgatccaa ctcggccagt accctgcccc
     3781 gtcccacacg atcgctcact tcagcgacac ccacctgctg gccggcggcc gtccgctgta
     3841 cggcaaagtc aacacgatcg accacctcgc ccaggcgctc gcccagctgg agcgatccat
     3901 cgccaagcct gaggcgatcg tcttcaccgg cgacctcgcc gacctcggtg agccggacgc
     3961 ctacgtgcgc ctgcgcgaac tcgtcgaacc tgccgccgcc cgcatgaatg cccgggtgat
     4021 ctgggtgatg ggcaaccacg acgaacgtgg cgactactcc cgactgctct tcgatgagga
     4081 atcggatgcg ccgcaggacc gcgtctacga catcaacgga ctgcgcatca tctccttcga
     4141 cacgacggtg cccggctatc accacggcga ggtcaccgac gatcagctcg actggctcgc
     4201 cgatgtgctc tccagccccg cgccccacgg cacccttctg gccgtgcacc acccacccat
     4261 ccccacgccg cttctcgagg ccatgggcat gctcgagctg ttcgatcagc acaagctggc
     4321 ggcggtactt gaggggtccg acgtgcgcgg catactcgcg gggcacctgc actactcgac
     4381 gcactcgacc ttcgcgggca tcccggtgtc ggtcgcgtcg gccacctgct acacgctcga
     4441 tctgagcgcc gaggaccgtc tgctctcggg cgtcgacttc ggtcagtcgg tgaacatcgt
     4501 gcacgtctac gacaagcagc tcgtgcactc gatcgtgccc atcggcgcga cgaccgaagt
     4561 gtctggattc ccggcgggag cctgggcgca gatcgaggcg atgacccccg aggagcgcat
     4621 cgagacgttc tccaagaagt cgtcgacgtt caactcggcc gaggtgtcga gcaacggtta
     4681 gcggggccgt tagggcaggg gatggtgacg ccgatccggc agtggtgatg ccggagatga
     4741 ggcggggatg acccggcgga tcgcgagaca cgcgtcgccg tagcatccag cgccgccccc
     4801 acggggcacc atggtcacgt ggacctcacc cgcgccgccg acgacttcgc gagctacctc
     4861 gcggccgacc gcggattcag cccccacacg gtgcgctcgt accgcagcga cctgtcgcag
     4921 ctggtcgact tcgccaccga gcgcggggcc gccgagtcac aggatctcga cctcgaactg
     4981 ctgcgcgact ggctgtggcg ctcgtcgcag gcgggtctcg ccaagtcgag tctcgcgcgc
     5041 cgcgccgcgg ccgcccgcag cttcaccgcc tggctcgctc gcacgaaggc ctccccggcg
     5101 gatgccgcca cccgcctgag ggcgcccaag agcgaccacc acctgccgcg ggtgctcacg
     5161 cgcgagcaga tcggcggcat cctcgaccgg gtcgccgaac gcgccgacac cggtgatccc
     5221 gtcgcgatcc gggatcgcgc gatcatcgag ttgctctacg cctcggccct gcgcgtcagc
     5281 gaactgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac gtcgaggatg
     5401 tcccgcagca gcgctgtcgg cgcgcgggcc gtcatccgct gtccgggccg ctcggttgtc
     5461 atgactgcca ctttaccgct atgttcctag attagttagc tactagagaa gttagagagt
     5521 catgcaccgc ctcacctcct tcatcaccgg tcgtcgcacg tcatggatcg tgctcgtgac
     5581 attcctcctc gctgccgggg ctgtcttcgc cgtcggctcg ggtgcgacaa gcgacacgtc
     5641 accgggcatc ggtctgccag cgtccgccga gtcggcacga gtcgccgcgg cccaggaaga
     5701 cctgccgagc gcggattcga ccagcgccct tctcgtcttc agtcgcgacg gcgcgaagct
     5761 gaccgatgcc gacatcgagg cggtcaccgc agcggcatcc gacctggcgg ggctctcgaa
     5821 ggacgacttc gtgccgccgc ccacggtctc cgacgacggc actgcagccc tggtcgtcgt
     5881 gccgctggag atcatcgagg atgttgcggc gcaagccgac cgcgccgagg agcttcgcac
     5941 caccgccaac gcagaccttc cgagcgggct gaccgctctg ctcaccggtg ccgagggctt
     6001 cgccgtcgac gttgccgccg tcttcaaggg tgctgatttc acgctgttgc tgacgacagt
     6061 gatcgtcgtt gcggttctgc tgctcatcac ctaccgcagc ccgtggctct ggctggtgcc
     6121 gctggtcgtc gtgggaaccg ccgacggcct cgcgagcatc gccgcgaccc gggtcgccgc
     6181 gcttgctggc atcaccctgg atgcctcggt gaccggcatc ctgtcggtgc tcgtcttcgg
     6241 tgccggcacc aactacgccc tgttgctgat cgcccgctat cgggacgagt tgcgcctgca
     6301 cgaggatcgt cgcgacgcca tggcccgcgc cctccgcggc gccggccccg cgatcatcgc
     6361 gagcggcagc accgtcacgc tcgccctgct caccctcgtc ttcgcccagc tcgagggcaa
     6421 ccgagccctc ggcatcgcct gtgcgaccgg tgtcgtgatc gcgatgctct tcgccctctt
     6481 cgtgctgccg gccgcgctcg tgctgttcgg acgcggactg ttctggccct acgtgccgcg
     6541 cttcggcagc gagggcagca tggaccgggg cgtctggcac cgtctcggtg tgctcgtgtc
     6601 gaagaagccc gtggtcgtcg cgatcgtcgg tgccgtcatc ctcggctcgc tcgccctcgg
     6661 caccacgcag atcaaggtcg gcctctcgca gaccgagcgc ttcacctcgg tgcccgaggc
     6721 agtcgtcggc cagaacatca tcgccgacgc gttctccgcc ggcagtggct cgcccgccgc
     6781 ggtcatcacg aacgccgact acgccgacaa ggtcgcctcg gtcgccgaga ctgtcgacgg
     6841 tgtcgactct gccaccgtcg gcgagacgaa cggcacgatc acccaggtca acgtggttct
     6901 ggatgccgcg gccgagaccc ccgagtcctt cgcagccatc gaggcactcc gcgccgccat
     6961 gcacgacatc gacggtgccg acgcgatcgt cggcggactc gacgcgcaga gcctggacgt
     7021 gacgcgcgcc cagaccgccg accaggatct ggtcatcccg ctgatccttg tgctcgtgtt
     7081 cctcgtgctg gtgctgctcc tgcgggcgct cgtggctccg gtgctgctgc tcgcgacggt
     7141 ggtggcctcg ttcttcgcga gtctcggcgc gagctggctg ctgttccagt cggtcttcgg
     7201 cttccccgcg atcgacacca acgtggtgct gttcagcttc ctgttcctcg tagcgctcgg
     7261 cgtggattac aacatcttcc tcgtcacgcg cgcccgggag gagacgcgcg agttcggcac
     7321 ccgtaccggc atgattcgcg ccctgtcgtc gaccggtggc gtgatcacca gtgcgggcat
     7381 cctgctcgcc gccgtgttcg ccgtgctcgg agtgctgccg ctgatcacgc tcacacagat
     7441 cggaatcatt gtctgcatcg gtgtgctgct cgacaccctc ctggtgcgca cggtgatcgt
     7501 gccgtcgctc gcgttcatca ccggcgagaa gttctggtgg ccgggtcgcc cgatgaccgt
     7561 tgccgcggag gtgcgcacca ccggctccgg cagctgaccg gcgcgggacg gcggtagctc
     7621 agccgcgtcg gggagagcgc cgacccgtgt cggcagcaac accgacgcgg gaatgccgcc
     7681 gaaccatgcc agcggtgaca cgtagtcgcc gttgacgcga acgcccacgt gcacgcacac
     7741 ctctgcgcag tgcccgggca ggatgcgccc gatcacctgc ccgcgcacga ccgggtcacc
     7801 ctgggcgacg gtggcgacca ccggctcgta actcgagatg acaccctcgc cgtgacgtat
     7861 cgagagcacc ggacggtcga ccacggttcc cgcgaagtgc acgaccccgt cggccggggc
     7921 gtagagcgtg ccctccggag cacggatgtc gactccccgg tgcccggcgg aatacgggct
     7981 ggcgggggcc agatagaccc gcgcgatcga acgaggtggg tcgacgggcc agcgccatga
     8041 gccggcctcg ggctcagcgg tgagcccgag gagggacgac ggcgccagga gcagcgtggc
     8101 ggccaggacg agggcgcgga gcatccaccg atcatcggcg tagccacgcc tcgacctgcg
     8161 tagcgcgtgg tatcggggga gaaccggccg tgagtgccgc ccgaggggga gcggtggtac
     8221 tattttcgaa gcactccggg caaccggggt gactacgcgt gtctacagct cctttcggga
     8281 gcgccgctca gcagagcgag gcccacaccc catcagtccc cgggaaaccg gggtggacgt
     8341 gtgcgcggac accaggttcg cggccgaccg gtcgcggaga acaactgaga acaggcgcgc
     8401 cgctgtgaaa acacgcgtgt gccgagaagg agcacggcca tggccgtagt aacaatccgc
     8461 cagctgctcg acagcggcgt ccacttcgga caccagaccc gccgctggaa
//
LOCUS       DAOSOU010000078         8340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8491_length_8340_cov_6.620640, whole genome shotgun sequence.
ACCESSION   DAOSOU010000078 DAOSOU010000000
VERSION     DAOSOU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8491_length_8340_cov_6.620640"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..706)
                     /gene="phoU"
                     /locus_tag="PLW15_05800"
     CDS             complement(50..706)
                     /gene="phoU"
                     /locus_tag="PLW15_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT33947.1"
                     /translation="MREVFQAELREVQDRLVEIATLVSVSIENATRAFNESNVSLAET
                     VIADDEKIDVAALELDELAIQILARQQPVARDLRIVVSALRISASLERMGDMAEHIAQ
                     LARYRFPDKVVPKSLRSTFAQLGELDVEIAKKLVELLKTEDAKLAEEIRNDDDKIDAL
                     HLSVFDKVLGETWKGAAVDTVDATLASRYHERFADHAVSIAKKVQYLATGDWVTPSAG
                     "
     gene            871..2028
                     /locus_tag="PLW15_05805"
     CDS             871..2028
                     /locus_tag="PLW15_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT33948.1"
                     /translation="MDSAWLVPAALAVGAVLGAGIFAIVMVAHARGQRAVAVVSSAVP
                     DGVDQVIDALESAGVVLDPSNNVVKASPGALAFGLVFNQALVHPELVKLVDTVRRNGE
                     PITEELSLSRGPFGEAVIHLSVRVARLGARYILLLAEDRTESYRLEEVRRDFIANISH
                     ELKTPIGAVGLLAEALVSASKEPEQVKRFAKRLTKESERLARITQEIIELSRLQATDA
                     LTKADLVDIDHVVARAIDQNRVVAEAHRVHLVSGGDAGAEVYGDEELLVTALHNLVAN
                     AIQYSPDGSRVGVGVNQSDGIVEIAVTDQGVGIEEDEQDRVFERFFRTDPARSRNTGG
                     SGLGLSIVKHVVQNHGGDVRVWSQPGSGSTFTIRLPEASHASAASLGAAAS"
     gene            2025..>2686
                     /locus_tag="PLW15_05810"
     CDS             2025..>2686
                     /locus_tag="PLW15_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT33949.1"
                     /translation="MTRILIVEDESSLSEPLAYLLEREGYETSIAADGLQAVADFDRT
                     GADLVLLDLMLPGMSGTEVCREIRTRSSVPIIMLTAKDSEVDIVVGLELGADDYVTKP
                     YSTRELLARIRAVLRRRVDTDLDDNQVLESGTVRMDVERHTVSVDGKDVPMPLKEFEL
                     LELLLRNSGRVLTRGQLIDRVWGADYFGDTKTLDVHIKRIRSKIEKVPGEPTMLVTVR
                     GLG"
     assembly_gap    2687..2786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2787..3001)
                     /locus_tag="PLW15_05815"
     CDS             complement(<2787..3001)
                     /locus_tag="PLW15_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33950.1"
                     /translation="MIARTPFARAAASVILASALVFGTTGCTFITSQATLNQYDPSDG
                     ISGRVGDLQLRNVLAIEGEDGKAVSLLL"
     gene            3101..3583
                     /locus_tag="PLW15_05820"
     CDS             3101..3583
                     /locus_tag="PLW15_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOT33951.1"
                     /translation="MLFEVGETVVYPHHGAATISEVKTRIVKGEEKLYLKLRVTQGDL
                     TIEVPAENVDLVGVRDVIGKEGLDKVFEVLRAPFTEEPTNWSRRYKANLEKLASGDVI
                     KVSEVVRDLWRRDQDRGLSAGEKRMLAKARQILISELALAEKTDEEKASSVLDEVLAS
                     "
     gene            3625..4806
                     /gene="ispD"
                     /locus_tag="PLW15_05825"
     CDS             3625..4806
                     /gene="ispD"
                     /locus_tag="PLW15_05825"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT33952.1"
                     /translation="MAEPGSRQARPPMVAVIVVAAGSGTRLGAGLPKAFVDVAGRSLL
                     EHALAAVPEAAQLVIVAPESHLAESRALAPRAVVVAGGDTRQASVAAGLAGVEPGIRT
                     VLVHDAARAFTPAEVFERVIAAVASTGGGVIPVLPVIDTIKRVDGASVLETIDRSALV
                     QVQTPQGFPREALDAAYRAATAEHTDDASLFAAAGGVVTSVAGDPRSHKITTPADLRR
                     AELEVGVPDSEGVSTGSTTGFRTGVGIDVHAYDAAEPLWLGGLYWPDEVGLSGHSDGD
                     AMCHAICDALLSAAGLGDIGSRFGTSDPRFAGAHGDVFLAETVAVVTGAGYSISNVSV
                     QLVANTPRLGERRSEIEAHLTQVVGAPVSVAATTSDGLGFTGKGEGVACVATALVQRA
                     G"
     gene            5126..6532
                     /gene="cysS"
                     /locus_tag="PLW15_05830"
     CDS             5126..6532
                     /gene="cysS"
                     /locus_tag="PLW15_05830"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT33953.1"
                     /translation="MTVRLYDSKAQGLVDFVPLVAGKVGMYLCGPTVQSSPHIGHLRS
                     ALVYDLWRRWFNYRGYDVTLVRNVTDIDDKILAAAAGDEAWWAHAYRYELEFTSGYTA
                     LGIQPPTYEPRATASVTEMQAIIQRLIEAGHAYPATDGSGDVYFDTASWPSYGELTRQ
                     KPENMEAATDADPRAKRDPRDFALWKGHKTDEPADAAWPSPWGDGRPGWHIECSAMSR
                     RYLGPAFDIHGGGLDLRFPHHENELAQSTAAGDAFATYWVHNGLVNINGQKMSKSLGN
                     SIYAAEFLELARPLVVRYFLGSAHYRSTIDYHDGALVEAEAALERIEVFLDRSERRLT
                     GTTHGVLEVIPDAFATAMDDDLSVPQALGVLHETVRAGNLAYDAGDLGALASARGEVL
                     AMVEVLGINPGSPQWAGAASGPTEQALASLVDSLIADRNAARAAKDFATADRIRDELA
                     AAGIAVEDGPTGTQWSVQ"
     gene            6564..7625
                     /gene="rlmB"
                     /locus_tag="PLW15_05835"
     CDS             6564..7625
                     /gene="rlmB"
                     /locus_tag="PLW15_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT33954.1"
                     /translation="MKNPGNKPRAGAVRKGRKGPQVGSGGQGRQALEGRKPTPKAEDR
                     PYHPAGKRKAAAERYEAAGGKKKVGSAAEGAGSRQARSPGAARPTGAARPGTGGRAPK
                     SSDESEMVTGRNSVLEALRAKIPATTLYMAARIEFDDRVKEILQLATSRGIPILEVMR
                     PELDRLAGFDSVHQGVALKVPPYEYAHPMELLDKVISRGQVPLLVALDGITDPRNLGA
                     IIRSVAAFGGHGVIVPQRRSVGLTASAWKTSAGAAARTPVAMAANLNQTIKDYKQRGV
                     FVLGLDGGGDVSLPQLQLAKEPVLIVVGSEGKGLSRLVTENCDAVVSIPINSGTESLN
                     AGIAASVTLYQISTLRAAK"
     gene            complement(7641..>8340)
                     /locus_tag="PLW15_05840"
     CDS             complement(7641..>8340)
                     /locus_tag="PLW15_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT33955.1"
                     /translation="KTIVVIGGTSGIGLEIAKDVVARGDKVVLSGRDLARAQEVAASI
                     GENATGVAVDLSEPHTIREGLKDVGTVNGLVLVAIERDANTVRDYDLDRATRLVVLKL
                     VGYTETIHVLSDRLVPDGSTGIVLFGGRAKDIPYPGSTTVSSINGGVEGMTVTLALEL
                     APIRVNALHPGIIGDSPFWAGKPAEVLDGHRRRTPGGHLAEMVDVVDATQFLLNNRGI
                     SGTSIYIDRGVRVT"
BASE COUNT         1324 a         2799 c         2834 g         1283 t
ORIGIN      
        1 gctactcaac cagcgggttg ccggtttcga tacgccgctt cgcggctact caaccagcgg
       61 agggggtgac ccagtctccg gtcgccaggt actgcacctt cttggcgatc gagaccgcgt
      121 ggtctgcgaa acgctcgtgg tagcgcgagg cgagcgtggc atccacggtg tcgacggcgg
      181 cacccttcca ggtctcgccg agcaccttgt cgaacacgga gaggtgcagc gcgtcgatct
      241 tgtcgtcgtc gttgcggatc tcctccgcga gcttcgcgtc ttcggtcttc agcagttcga
      301 cgagcttctt ggcgatctcg acatccagct caccgagctg cgcgaacgtc gagcgcaggc
      361 tcttcggcac aaccttgtcg gggaagcggt agcgcgccag ctgagcgatg tgctcggcca
      421 tgtcgcccat gcgctccagc gacgcggaga tgcgcagcgc cgacaccacg atgcgcaggt
      481 cgcgggcgac cggctgctgg cgggccagga tctggatggc cagctcatcg agctcgaggg
      541 cggcgacgtc gatcttctcg tcgtcagcga tcaccgtctc ggccagagac acgttggact
      601 cgttgaaggc gcgagtcgcg ttctcgatcg ataccgatac cagagtcgca atctcgacta
      661 gccggtcctg cacttctcgc agttcggcct gaaaaacttc gcgcacgttg aaagtccctt
      721 cgctacgggc atgagactac ccgcacggcg ttgtcagact gcccgcgtca ggtgaacggc
      781 aggtgttgca cgcctgaaca tcacccaaag tgcctcggct ttcgccaacc agcgcaaggc
      841 cgcacggcag atgtaactaa tctagtcgct atggactccg cgtggctggt gcccgccgct
      901 ctcgccgttg gcgcggtgct cggcgcaggc atcttcgcga tcgtcatggt cgcccacgcc
      961 cgcggtcaga gggcggttgc ggtcgtcagc tccgctgttc cggatggcgt cgatcaggtg
     1021 atcgacgctc tcgagtccgc cggcgtcgtg ctcgacccct ccaacaacgt cgtgaaagcc
     1081 tcgccgggtg ccctggcgtt cggcctcgtc ttcaaccagg cgctggtgca tccggagctc
     1141 gtcaaactcg tcgacaccgt gcgccgcaac ggcgagccca tcaccgaaga actcagcctc
     1201 agccgcggcc cgttcggcga ggcggtcatc cacctgtcgg tgcgggtggc gcgcctgggc
     1261 gcgcggtaca tcctgctgct ggccgaggac cgcaccgagt cgtaccgcct cgaggaggtg
     1321 cgccgcgact tcatcgccaa catcagccac gagctgaaga ccccgatcgg cgccgtcggc
     1381 ctgctcgccg aggccctggt gagcgcatcc aaggagccgg agcaggtcaa gcgcttcgcc
     1441 aagcgcctca ccaaggagag tgagcgtctc gcgcgcatca cgcaggagat catcgaactc
     1501 tcccgccttc aggcgacgga tgccctcacc aaggccgacc tcgtcgacat cgaccacgtg
     1561 gtcgcccgag cgatcgacca gaaccgcgtc gtcgcggaag cccaccgcgt gcacctcgtc
     1621 tccggcggtg acgcgggtgc cgaggtgtac ggcgacgagg agctgctggt gaccgcgctg
     1681 cacaatctgg tggccaacgc gatccagtac tcaccggatg gctcgcgggt gggtgtcggc
     1741 gtcaaccaga gtgacggcat cgtggagatc gcggtgaccg accagggcgt gggcatcgag
     1801 gaggacgagc aggatcgcgt cttcgagcgg ttcttccgca ccgacccggc gcgcagtcgc
     1861 aacacgggcg gcagcgggct gggcctgagc atcgtcaagc acgtcgtgca gaaccatggc
     1921 ggcgacgtgc gggtctggtc gcagccgggc agcgggtcca ccttcaccat tcgacttccc
     1981 gaggcatccc atgcttcggc cgcatcactg ggagcagcag cgtcatgacc cgaatcctga
     2041 tcgtcgaaga cgagtcgtcg ctgagcgagc cgctcgccta cctgctggag cgcgaaggct
     2101 acgagacgtc gatcgcggcc gacggcctgc aggcggtcgc cgacttcgac cgcactggcg
     2161 ccgacctcgt gctgctcgac ctgatgctgc ccgggatgag cggcaccgag gtgtgccggg
     2221 agatccgcac ccgctcgtcg gtgccgatca tcatgctgac cgcgaaagac agcgaggtcg
     2281 acatcgtcgt gggactcgag ctgggtgccg acgactacgt gaccaagccg tactcgaccc
     2341 gggaactgct ggcgcgcatc cgtgccgtgc tccgtcgccg ggtcgacacc gacctcgacg
     2401 acaaccaggt gctcgagtcg ggcaccgtgc gcatggatgt ggagcgccac accgtgagcg
     2461 tcgacggcaa ggacgtgccg atgccgctca aggagttcga gctgctcgag ctgctgctgc
     2521 gcaactcggg ccgggtgctc acgcgcggcc agttgatcga tcgggtgtgg ggcgccgact
     2581 acttcggaga cacgaagacc ctcgacgtgc acatcaagcg gattcgctcg aagatcgaga
     2641 aggttccggg cgaaccgaca atgctggtta ccgtgcgcgg gctgggnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnagca gaaggctcac ggccttgccg tcttcgcctt
     2821 cgatcgcgag cacgttgcgc aactgcaggt cgccgacgcg gccgctgatg ccatcgctcg
     2881 ggtcgtactg gttgagtgtc gcctggctgg tgatgaacgt gcatccggtc gtaccgaaga
     2941 ccagtgctga tgccagaatc acggatgccg cagcgcgggc gaagggggtg cgtgctatca
     3001 cgcctcaagc ataccgaccg gggcatgcgg gcgctgagtt aggacagcct taccatgaaa
     3061 cccctgtggt aaactgggtg tcttcgaaag ggtcccccac atgttgtttg aggtcggcga
     3121 gactgtcgtt tatccccacc acggtgcagc aaccatctcc gaggtgaaga cgaggatcgt
     3181 caagggcgag gagaagctct acctcaagct gcgcgtcact cagggcgatc tgaccatcga
     3241 ggttcctgct gagaacgtcg acctggtcgg cgttcgcgac gtgatcggca aggagggcct
     3301 cgacaaggtc ttcgaggtgc tgcgcgcccc gttcaccgag gagcccacca actggagccg
     3361 ccgctacaag gccaacctcg agaagctcgc ctcaggcgac gtcatcaagg tctccgaggt
     3421 cgtgcgcgac ctgtggcgtc gcgaccagga ccgcggcctg tccgccggtg agaagcgcat
     3481 gctcgcgaag gcccgccaga ttctgatctc cgagctcgcg ctcgccgaga agactgacga
     3541 ggagaaggcc tcgagcgttc tcgacgaggt tctggcctcc taggcttccg gctggttgag
     3601 tagcgcgaag cgcgtatcga aaccatggct gaacccgggt ctcgacaggc tcgaccaccg
     3661 atggtcgcgg ttatcgtcgt cgccgccggc tccggcacgc gcctcggcgc tggcctgccc
     3721 aaagccttcg tggacgtcgc cgggcgctcg ctgctcgaac acgcgctcgc cgccgtgccc
     3781 gaggccgcac aactcgtgat cgttgcgccc gagtcgcacc tcgcggaatc ccgggccctc
     3841 gcgccccgcg ccgtggttgt tgctggtggc gacacccggc aggcgtcggt tgcggcgggg
     3901 ctcgctggtg ttgagccggg catccgcacc gtgctcgtgc acgacgcggc acgcgcgttc
     3961 acgccggcgg aggtgttcga gcgggtgatc gccgcggtcg cctcgacggg tggcggcgtc
     4021 atcccggtgc tgccggtgat cgacaccatc aagcgcgtcg acggtgcctc cgtgctcgag
     4081 acgatcgacc gctcagcact ggtgcaggtg cagacgccgc agggcttccc ccgcgaggct
     4141 ctggatgccg cgtaccgcgc ggcgaccgcg gaacacaccg acgacgcctc cctcttcgcc
     4201 gccgccgggg gagtggtcac ctctgtcgcg ggggaccccc gctcccacaa gatcacgacg
     4261 cccgccgacc tgcgccgggc cgaactggag gttggtgtgc cggactctga gggcgtctcg
     4321 acaggctcga cgaccgggtt ccgcaccggc gtcggaatcg acgtgcacgc gtacgacgcg
     4381 gccgagccgc tctggctggg tgggctgtac tggcccgacg aagtggggct ctccggccac
     4441 tccgacggcg acgcgatgtg ccacgcgatc tgcgacgcac tgctgtcggc ggccggcctc
     4501 ggcgacatcg gatcacggtt cggcacgagc gacccgcggt tcgcgggggc gcacggcgac
     4561 gtgttcctcg ccgagaccgt tgccgtggtg acgggtgccg gctactcgat ctccaacgtg
     4621 tcggtgcagt tggtggccaa cactccgcgg ctcggggagc gccgcagcga gatcgaagcg
     4681 cacctgactc aggtggtggg ggctcccgtg agcgtggcgg ccacgacatc cgacggcctc
     4741 ggtttcacgg gcaagggcga gggcgtcgcc tgcgtcgcga ccgcgctggt gcagcgcgcc
     4801 gggtaagcgc gccgggcaag tgtagtgcgg cgggccgctc ggagtcggcg cggtgctcgc
     4861 gcggtggtgc tcgcgcgaga gcttctcgcg cgctcggctc gcgccagtct ccgcgtcgcg
     4921 actcctgttg tgcaggacag gcgaggctcg ctcgtgtgtc ctgcacaaca gaagtgtgca
     4981 gcgggggagg cgcttgcgag cgggtcagcg gaagcgggtc agcacgagcg ggtgctcagg
     5041 cggggcgcgg gagctggagc gcgggccagc gagagcgcga gccgagcatc cgtcatcggc
     5101 cgcggcgccc cggtaaactt gcccagtgac agtccgcctc tatgactcga aggcccaagg
     5161 cctcgtcgac ttcgtgccgc tcgtggcagg caaggtcggc atgtacctct gcggacccac
     5221 cgtgcagtcg agcccccaca tcggccacct gcgctccgcg ctcgtctacg acctgtggcg
     5281 gcgctggttc aactaccgcg gctacgacgt gacgctcgtg cgcaacgtca ccgacatcga
     5341 cgacaagatc ctggcggccg ccgcgggaga cgaagcatgg tgggcgcacg cctaccggta
     5401 cgagctcgag ttcacgtccg gctacaccgc gctgggcatc cagcccccca cctacgagcc
     5461 ccgcgccacc gcgagcgtca ccgagatgca ggcgatcatc cagcgcctca tcgaggccgg
     5521 ccacgcctat ccagcgacgg atggctcggg cgacgtctac ttcgacacgg cgagctggcc
     5581 ttcctacggc gagctcactc gccagaagcc cgagaacatg gaggcggcga ccgacgccga
     5641 cccgcgcgcc aagcgcgacc cccgcgactt cgccctgtgg aagggccaca agacagacga
     5701 gccagcggat gccgcctggc cgagcccctg gggcgatggc aggccgggct ggcacatcga
     5761 gtgctccgcc atgtcacgcc gctacctggg acccgccttc gacatccacg gcggcggact
     5821 cgacctgcgc ttcccgcacc acgagaacga gctcgcccag tcgaccgccg cgggcgacgc
     5881 tttcgcgacc tactgggtgc acaacgggtt ggtcaacatc aacgggcaga agatgtcgaa
     5941 gtccctcggc aactcgatct atgccgccga gtttctcgaa ctcgcccgcc cgctcgtcgt
     6001 gcgctacttc ctcgggtccg cccactaccg ctcgacgatc gactaccacg acggcgccct
     6061 cgtcgaagcc gaggcagccc tcgagcgcat cgaggtcttc ctcgaccgct ccgaacgccg
     6121 gctgaccggc accacccacg gggtgctcga agtgatcccg gatgcgttcg ccaccgccat
     6181 ggacgatgac ctgtcggttc cgcaggccct cggtgtgctg cacgagacgg tgcgcgcggg
     6241 caacctcgcc tacgacgccg gtgacctggg cgcgctcgcg tccgcccgcg gcgaggtgct
     6301 ggcgatggtc gaggtgctgg ggatcaaccc ggggtcgccg cagtgggcgg gcgcggcatc
     6361 cggacccacc gagcaggcgc tcgccagcct tgtcgactcg ctcatcgccg accgcaatgc
     6421 ggcccgcgcc gcgaaggatt ttgccacggc cgaccggatc cgtgatgaac tggcggctgc
     6481 gggaatcgcg gtcgaggacg gcccgaccgg aacacagtgg agcgtgcagt gactgcgcgg
     6541 cactcgcaca aggagcgaaa ctgatgaaga acccaggcaa caagccccga gcgggcgccg
     6601 tgcgcaaggg ccgtaagggc ccacaggtcg gctccggcgg gcaggggcgc caggcgctcg
     6661 agggacgcaa gccgaccccc aaggccgagg accgcccgta ccaccccgcg ggcaagcgca
     6721 aggctgcggc cgagcggtat gaggcagcgg gcggcaagaa gaaggtcggc tcggctgctg
     6781 agggtgcggg atctcgacag gctcgatcac cgggggccgc tcgaccgacg ggggcggctc
     6841 ggcccggcac cggaggccgc gcgccgaagt cgagcgacga gtcggagatg gtgaccggtc
     6901 gcaactccgt gctcgaggcg cttcgcgcca agatcccggc gacgacgctc tacatggcgg
     6961 cccgcatcga gttcgacgac cgcgtcaagg agatcctgca gctggcgacg agccgcggca
     7021 tcccgattct cgaggtcatg cgccccgagc tcgaccgcct cgccggcttc gactcggtgc
     7081 atcagggcgt cgcgctgaag gttccgccgt acgagtacgc gcacccgatg gagctgctcg
     7141 acaaggtgat ctcccgcggc caggttccgc tgctggtcgc gctcgacggc atcaccgacc
     7201 cgcgcaacct gggtgcgatc atccgctccg tcgccgcgtt cggtgggcac ggtgtgatcg
     7261 tgccgcagcg gcgctcggtg ggtctgacgg cctcggcgtg gaagacctcg gcaggtgccg
     7321 cagcgcgcac cccggtcgcg atggccgcca acctcaacca gacgatcaag gactacaagc
     7381 agcgcggcgt cttcgtgctc ggcctcgacg gcggcgggga tgtctcgctg ccgcagcttc
     7441 agctcgcgaa ggagcccgtg ctcatcgtcg tgggcagcga gggcaagggc ctgtcgcgac
     7501 tggtgaccga gaactgcgac gccgtggtct cgatccccat caactcggga accgagtcgc
     7561 tcaacgcggg catcgcggcg agcgtgacgc tgtaccagat ttcgactctg cgcgccgcga
     7621 agtagcgccg cctgtgcggg tcaggtgacg cgcacgcccc ggtcgatgta gatgctcgtg
     7681 cccgagatgc ccctgttgtt cagcaggaac tgcgtggcat ccacgacgtc gaccatctcg
     7741 gcgagatgtc cacccggcgt gcgccgacgg tgaccgtcga gcacctcggc gggcttgccg
     7801 gcccagaacg ggctgtcgcc gatgatgccg gggtgcaggg cgttcacgcg gatcggcgcg
     7861 agctccaggg cgagggtcac ggtcatgccc tcgacaccac cgttgatgga tgacacggtc
     7921 gtcgagcccg ggtacgggat gtccttggcg cgcccgccga agagcacgat gccggtgctg
     7981 ccgtcgggca cgaggcggtc ggacagcacg tggatcgtct cggtgtagcc gacgagcttg
     8041 agcacgacga ggcgcgtggc ccggtcgagg tcgtagtcgc gcacggtgtt ggcgtcgcgc
     8101 tcgatcgcca cgagcacgag gccgttgacg gttccgacat ccttgagacc ctcgcggatg
     8161 gtgtgcggct cagacaggtc gacggcaacg ccggtggcgt tctcgccgat ggatgccgcg
     8221 acttcctggg cgcgagcgag gtcacgcccc gagagcacca ccttgtcgcc gcgcgcgacg
     8281 acatccttgg cgatctcgag gccgatgccg gacgttccgc cgatgacgac gatggtcttc
//
LOCUS       DAOSOU010000079         8221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8673_length_8221_cov_5.365663, whole genome shotgun sequence.
ACCESSION   DAOSOU010000079 DAOSOU010000000
VERSION     DAOSOU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8673_length_8221_cov_5.365663"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLW15_05845"
     CDS             <1..574
                     /locus_tag="PLW15_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33956.1"
                     /translation="LLPGGGLQQGAAYSVERSAMLVMTLLAGPSAAGSWCAVVGVPEF
                     GVEAASRFGIDLERLVLVPHPGDQWLAVTAAVADVMGVVVTRPPKQASDASVARLSAR
                     LRQRGATLLVLGTWPQSEAMLSLSDSEWFGVGEGHGHLAARQVTVTVTHRLSGRPRSA
                     RLWLPAADETVQRVSRAGGVASAAPLAATG"
     gene            571..2154
                     /locus_tag="PLW15_05850"
     CDS             571..2154
                     /locus_tag="PLW15_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOT33957.1"
                     /translation="MNRRPAAAVGTGTDPGAGLPRVVVLWCPDWPVTAAIRAAGLPAD
                     APVALIAKGVVFACSASARAEGIARGLRLREAQARLPHLTALDYDPALDNRAFEPLIA
                     AVEHLVPGVQLVRPGVCAVRIRGAARYYGGERAAALALTSQLDELGAGGARAGIGDGL
                     FTAEQAARRANPVHIVAEGGAAEFLAPLDVGLLEEGQPGIVTLLRRLGIRTLGEFAQL
                     GADDVRLRFGEQGARVHALSGGRDSLPLSPRTPPRDLDVIVDFEPALDRVDQVAFGVR
                     AAAERFIDDLTAVKLVCTAIRVELDSDSDERSERVWLHPRSFTAADVVDRVRWQLQGS
                     GVIDAALSSGITRVSVAPEAVDAIGNHETGLWGAGPDERVHHGLSRVQSMLGHGAVLV
                     PVLSGGRTLADRQQLVAWGDRPVAGRSAAEPWPGRLPDPLPGTVFVPRHPVHVLSKAG
                     EPVAMDARGTLTAPPRRFSAGRTELTITRWAGPWPLDERWWTSGARAAWRFQAVDSSG
                     CAWLLVLDSGGWWAEARYD"
     gene            2156..5548
                     /locus_tag="PLW15_05855"
     CDS             2156..5548
                     /locus_tag="PLW15_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOT33958.1"
                     /translation="MGWSNPPIPWSEFERNLSDLSRPGSAPPVGADGGDSPAWSYKRK
                     GYVPPADLALAGPAVVPYAELHAHSSFSFLDGASMPESLAEEAARLGLTALALTDHDG
                     LYGIVRMAEAAASIGTTATIFGAELSLGLTGPQNGVPDPEGSHLLVLARKEEGYHRLA
                     AAITRGQLRGGEKGRPVYDLEELAEQSGGHWLILTGCRKGTVRQALEQGHAGAELDRL
                     VALFGADNVVVELWDHGNPLDSTHNDALYELAAQKRLRVVATSNAHYATPEQHHLHSA
                     LAAVRARRSLDEMDGWLPAAGTAHLRSGAEMAVRFARYPGAVENTVVIADELSFELRK
                     AKPALPDQQVPPGHTPMSYLRELVWAGLPDRQIDPTPENLARIGKELDAIEAKHFAGY
                     FLIVWDIVREARSRGILCQGRGSAASSVVCYLLKITAVDPIRYKLPFERFISTMREEE
                     PDIDVDFESERREEIIQYVFDKYGRENAAQVANVIQYRPKFAVRDMAKAMGHSPGQQD
                     AWSKQVERWGATVSSADHDIPDSVVGLAMEVMKFPRHLGIHSGGMVLTQRPVGEVVPI
                     EHARMEKRTVVQWDKDDCAWMGLVKFDLLGLGMLSALQYSFDLVRDALGESHDLDTIP
                     KEEQGVYDQLCRADAIGVFQVESRAQMGLLPRLQPREFYHLVVEIALVRPGPIQGGAV
                     HPYVRRKLGQEEITYLHPKLVEPLERTLGIPIFQEQLMQIAVAVGGCTPDDADLLRRA
                     MGSKRGVEKIESLKAKLYEGMAGNGIVGADADNIYARIQAFAGFGFAESHALSFGLLV
                     YASAWMRLHYPAAFLAALLRAQPMGFYSPQSLVADARRHGVTVLRPDIQRSGVYATLE
                     PVAGVEPTGRPECLHREQPPIGPFDRSSPDTTAEHRRDGALAVRLGLDEVTGIGTAVA
                     KRIVAERERGGDYRDMNDLVRRVDLTTAQLETLSAAGAFDTFGLSRREALWNAGNAAQ
                     DRAQYLPGTIVAVQPPLFAMPTAVEELMSDLWATGVSTDSHPVQHLRESLTDRGVLSA
                     ADLVTAESGRRIEIGGVVTHRQRPATASGITFLNIEDETGLVNVICSVGVWDRYRRVV
                     REAPALIVRGILERSAEGVTNVLADRFEVLAMAPRTRSRDFR"
     gene            5705..5908
                     /locus_tag="PLW15_05860"
     CDS             5705..5908
                     /locus_tag="PLW15_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT33959.1"
                     /translation="MALGTVKWFNAEKGFGFIAPEDGSPDVFAHYSAIATGGYKSLDE
                     NQKVEFDITQGPKGPQAENIRPL"
     gene            complement(5980..7764)
                     /locus_tag="PLW15_05865"
     CDS             complement(5980..7764)
                     /locus_tag="PLW15_05865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT33960.1"
                     /translation="MSTPPPSTDFPTPTVGTFAAEHLSPSYPERAARGTADKLRAWQA
                     EALDAYFDREPKDFLAAATPGAGKTTFALRLAVELIARRVVDRVTVVAPTDHLKRQWA
                     DAAHKVGLRLNPAYANGDGYGGRRFHGMAVTYAQVAVRASLHREITESARTLVILDEV
                     HHGGDALSWGDAIREAFEPATRRLSLTGTPFRSDTAPIPFVSYLADEKGIRTSLTDYS
                     YGYGRALADGVVRPVIFLAYAGKMRWRTRMGDEMEARLGEGDTQDITAQAWRTALDAK
                     GEWMPAVLRAANTRLTEVRHSIPDAGGLVIATDQQAARAYAALLKDITGEPATVVLSD
                     EKASSARIEKFAAGESRWMVAVRMVSEGVDVPRLAVGVYATSASTPLYFAQAVGRFVR
                     ARRRGETASIFLPSVPSLMSLATQLELERDHALDREHDDTKLLEDELLERESQQEATS
                     DELTDEFTWEAIESDANFDKVLFDGDEFGFAALTGSDEELDFIGLPGILEPDQVRELL
                     KQRQARQSRRAASKPREEQPPPLYRTLKEQRSLLNSLVGMRSKLSGEPHGLIHAELRR
                     LCGGPPVAQATVTQLQARIDHLRRGMKR"
     gene            complement(7827..>8221)
                     /locus_tag="PLW15_05870"
     CDS             complement(7827..>8221)
                     /locus_tag="PLW15_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT33961.1"
                     /translation="MSSMVAVRLRKAGMRGRTVVLKLRYEDFTTITRSRTLPEPTDLG
                     RTIYDEVRSIYESLGNDRPVRLVGVRAEQLLDAADVQLGLWDETDGWREAEDVMDAAS
                     ARFGRGAVTPARLIGHAATERPRLGQRD"
BASE COUNT         1207 a         2805 c         2899 g         1310 t
ORIGIN      
        1 gctactgccc ggcggcggtc tccagcaggg tgccgcctac tcggtcgagc gttcggcgat
       61 gctcgtcatg accctgctgg ccgggccgtc ggctgcgggc tcgtggtgcg ctgtcgtggg
      121 ggtcccggag ttcggcgttg aagcggcatc ccgattcggt attgacctcg agcggctggt
      181 gctggtgcct caccccggcg atcagtggct cgccgtgacg gctgcggtag cggatgtgat
      241 gggtgtcgtc gtcacgcgtc cgccaaagca ggccagtgat gcgagtgtcg cccggctctc
      301 ggcccgcctg cgccagcgcg gtgcgacgct gctcgtgctc ggcacctggc cgcagagcga
      361 ggcgatgctg tcgctgtcag acagcgagtg gttcggggtg ggcgaagggc acgggcatct
      421 ggccgcgcgc caggtgacgg tcaccgtgac ccaccggctg agcgggcgcc ccaggagtgc
      481 gaggctgtgg cttcccgccg ccgacgagac ggtgcagcgg gtctcgcggg ccgggggcgt
      541 cgcatccgct gccccgctgg cggcgaccgg atgaacaggc gacccgccgc cgccgtcggc
      601 actggcaccg acccgggtgc gggtctgccg cgagtggtgg tgctgtggtg cccggactgg
      661 ccggtcaccg cggcgatccg tgcggcaggg ctgccggcgg atgccccggt cgccctgatc
      721 gcgaagggcg tcgtcttcgc gtgctcggcc tccgctcgcg ccgaagggat cgcgcgcggc
      781 ctgaggctgc gcgaagctca ggcccgcctg ccgcacctga ccgcgctcga ctacgatccc
      841 gcgctcgaca accgcgcctt cgaaccgctc atcgccgccg tggagcacct ggttccgggc
      901 gtgcaactgg tgcgccctgg cgtgtgcgcc gtgcgcatcc gcggtgccgc ccgctactac
      961 ggcggcgaac gggcggcggc gctcgcgctc acctcccaac tcgacgagct cggcgccggc
     1021 ggggcacgcg cgggtatcgg cgacggactc ttcactgccg agcaggcggc gcgccgggcc
     1081 aatcccgtgc acatcgttgc cgagggtggg gcggccgagt ttctcgctcc gctcgacgtg
     1141 gggttgctgg aggaggggca gccgggcatc gtcacgctgc tgcggaggct gggcatccgc
     1201 accctcgggg agttcgcgca actcggtgcc gacgatgtgc gcctgcggtt cggcgaacag
     1261 ggtgccaggg tgcacgcgct gtcgggcggc cgcgacagcc tgcctctgag cccgcgcact
     1321 cccccgcgcg atctcgacgt gatcgtcgac ttcgagccgg ccctcgaccg tgtcgaccag
     1381 gtcgccttcg gggtgcgtgc cgcggcggag cggttcatcg acgacctcac cgcggtcaag
     1441 ctggtctgca cggccatccg ggtggagctc gactccgact ccgatgagcg ctcggagcgg
     1501 gtctggctgc acccgcgatc cttcacagct gccgacgtcg tcgaccgagt tcgctggcag
     1561 ttgcagggtt caggcgtgat cgatgccgcc ctcagctccg gcatcacacg ggtcagtgtg
     1621 gctcccgagg ccgtcgacgc gatcggcaac cacgagaccg gcctgtgggg ggcgggtccc
     1681 gacgagcgcg tgcaccacgg gctctcccgg gtgcagagca tgctcggcca cggcgccgtg
     1741 ctcgtgcccg tgctgtcggg gggccgcacc ctcgccgacc gccagcagct ggtggcgtgg
     1801 ggcgaccgcc cggtggccgg ccgctccgcg gccgaaccgt ggccgggcag gctgcccgac
     1861 ccgctgccgg gcacggtgtt cgtgccgcgg catccggtgc acgtgctgtc gaaggcgggc
     1921 gagcccgtcg cgatggatgc ccggggcacc ctcacagcgc cgccccgccg tttctccgcc
     1981 ggtcgtaccg agctcacgat cacgcgctgg gcgggaccgt ggccgctcga cgaacgctgg
     2041 tggacctctg gcgcccgtgc cgcctggcgg ttccaggccg tcgactcgtc ggggtgcgcc
     2101 tggctgctcg tgctcgactc cggcggctgg tgggccgaag cgaggtacga ctgagatggg
     2161 ctggtccaac ccacccatcc cctggtcgga gttcgagcgc aacctctccg acctgtcccg
     2221 gccgggctct gctcccccgg tgggcgccga tggtggtgac agcccggcgt ggtcgtacaa
     2281 acgcaagggc tacgtgcctc ccgccgatct cgctctggcc ggccctgctg tggtgccgta
     2341 cgcggagctg cacgcccact ccagtttcag cttcctcgac ggggcgagca tgcccgagtc
     2401 gctcgccgag gaggcggcgc ggctggggct gaccgcactg gccctcaccg accacgacgg
     2461 tctgtacggc atcgtgcgca tggcggaggc tgccgcctcg atcggcacca cggccacgat
     2521 cttcggtgcc gagctctcgc tggggctcac cggtccgcag aacggcgtgc ccgatcccga
     2581 gggcagtcac ctgctcgtgc tcgcccgcaa ggaggagggg taccaccggc tggcggcggc
     2641 catcacgcgc gggcaactgc gcggtgggga gaagggtcgg cccgtgtacg acctcgagga
     2701 gctcgccgaa cagtcgggcg gtcactggct gatcctgacc ggatgccgca agggcaccgt
     2761 gcgccaggcg ctcgagcagg gccacgccgg agccgaactc gaccgcctcg tcgccctgtt
     2821 cggcgccgac aacgtcgtcg tcgagctgtg ggatcacggc aacccgctcg acagcaccca
     2881 caacgacgcg ttgtacgagc tcgcggcgca gaaacggctg cgcgtggtcg cgaccagcaa
     2941 cgcgcactac gccacccccg agcagcatca cctgcactca gccctcgcgg cggtacgcgc
     3001 ccggcgcagc ctcgacgaga tggacggctg gctacccgcc gccggcaccg cccacctgcg
     3061 cagcggcgcg gagatggccg tcagattcgc ccgatacccg ggtgccgtgg agaacacagt
     3121 ggtcatcgcc gatgaactga gtttcgagct gcgcaaggcc aagcccgccc tgcccgacca
     3181 gcaggtgccg cccggacaca ctcccatgag ctacctgcgc gagctggtct gggcgggcct
     3241 tcccgatcgg cagatcgatc cgacacccga gaatctggcc cgcatcggca aggagctgga
     3301 tgccatcgag gccaagcact tcgcggggta cttcctgatc gtgtgggaca tcgtgcgcga
     3361 ggccaggagc cgcggcatcc tgtgtcaggg tcgcggatcg gcggccagca gcgtcgtctg
     3421 ctacctgctc aagatcaccg ccgtcgatcc gatccgctac aaactgccgt tcgagcgctt
     3481 catctccacc atgcgcgagg aggagcccga catcgatgtc gacttcgagt cggagcgccg
     3541 cgaggagatc atccagtacg tcttcgacaa gtacggtcgg gagaacgcgg cgcaggtggc
     3601 caacgtcatc cagtaccggc ccaagttcgc ggtgcgcgac atggccaagg cgatggggca
     3661 cagccccggc cagcaggatg cgtggtcgaa gcaggtggag cgctggggtg cgactgtctc
     3721 cagcgccgac cacgacatcc cggactcggt ggtgggtctg gccatggagg tcatgaagtt
     3781 cccccggcac ctgggcatcc actcgggcgg catggtgctc acccagcggc cggtcggtga
     3841 ggtggttccg atcgagcacg cgcgcatgga gaagcgcaca gtcgttcagt gggacaagga
     3901 cgactgcgcc tggatgggcc tggtcaagtt cgacctgctc ggcctcggca tgctctcggc
     3961 gctgcagtac agcttcgatc tggtgcgcga cgcactgggc gagtcgcatg acctcgacac
     4021 gatccccaag gaggagcagg gcgtctacga ccagctgtgc cgggcggatg cgatcggggt
     4081 cttccaggtg gaatcccgcg cccagatggg actgctgccg aggcttcagc cgcgcgagtt
     4141 ctaccacctg gtcgtcgaga tcgcgctggt gcggccaggt cccatccagg gcggagcggt
     4201 gcatccgtat gtcaggcgca agctcgggca ggaggagatc acctacctgc accccaagct
     4261 cgtcgaaccc ctggagcgca cgctgggcat cccgatcttc caggagcagc tcatgcagat
     4321 cgccgtcgcc gtgggcggct gcaccccgga tgacgccgac ctgctgagac gcgcgatggg
     4381 ctccaagcgc ggggtggaga agatcgagag tctcaaggcc aagctctacg agggcatggc
     4441 cggcaacggc atcgtgggcg ctgacgccga caacatctac gcgcgcatcc aggccttcgc
     4501 gggcttcggc ttcgccgaga gccacgccct gagcttcggt ctgctggtct acgcgagtgc
     4561 gtggatgcga ctgcactacc cggccgcgtt cctcgccgcc ctgctgcgcg cgcagccgat
     4621 gggcttctac tcgccgcaat ccctggtggc ggatgcccgg cgccacggtg tcacggtgct
     4681 gcggcccgac atccagcgct ccggggtgta cgcgacgctc gagccggtgg caggggtcga
     4741 gccaaccggc cgaccggagt gcctgcaccg cgagcagccg ccgatcggtc cgttcgaccg
     4801 ttcctctccc gataccaccg ccgaacaccg ccgcgacggg gctctggcgg tgcgcctggg
     4861 gctcgacgag gtcaccggca tcggcacggc agtggccaag cggatcgtcg ccgagcgcga
     4921 gcgcggcggc gattaccggg acatgaacga cctcgtgcgc cgggtcgatc tgacgacagc
     4981 gcaactcgag accctctcgg ccgcgggggc cttcgacacc ttcggtctga gtcgccgcga
     5041 ggcgctctgg aacgcgggca acgccgcaca ggatcgtgcg cagtatctgc cgggaacgat
     5101 cgtcgccgtg cagccgccgc tgttcgccat gccgacagcc gtggaggagc tgatgagcga
     5161 cctgtgggcg accggcgtct ccacagacag tcatcccgtg cagcatctgc gggagagtct
     5221 gaccgaccgg ggcgtgctgt cggcggccga tctcgtgacc gccgagtcgg ggcgtcgcat
     5281 cgagatcggc ggggtggtca ctcaccgcca gcgacccgca accgccagcg gcatcacctt
     5341 cctcaacatc gaggacgaga cgggactcgt caacgtcatc tgcagcgtgg gggtctggga
     5401 tcgctatcgg agggtggtgc gtgaggcacc ggcgctcatc gtgcggggca tcctcgagcg
     5461 ctcggcggaa ggagtcacga acgtgctcgc cgatcgcttc gaggtgctcg cgatggctcc
     5521 gcgcacccgt tctcgcgatt tccggtaggt gcacgccaga tgacgattcg ttaccggttg
     5581 tgaaatacat ggcactatca gccgactact gctatagcct tggcacatcg tcggtgcact
     5641 cctcggaggt atcgatgtgg cctcgactgt cggggccaga agaacgctcc tggaggagtt
     5701 ttacatggca ttaggtaccg ttaagtggtt caacgcggaa aagggctttg gattcatcgc
     5761 ccctgaggat ggaagtcctg acgtgttcgc ccactactcg gccatcgcaa ccggtggcta
     5821 caagtcgctc gacgagaacc agaaggtcga gttcgacatc acccagggac cgaagggtcc
     5881 ccaggcggag aacatccgcc ccctctgatc ttgtaaatca gtaacgctgt gcaaacagca
     5941 cagaaacgcc cccggtccta taccgggggc gtttctctgt taacgcttca ttccacgacg
     6001 caggtggtcg atcctcgcct gcaactgcgt gacagtggcc tgcgcgacgg gcggtccgcc
     6061 gcacagccgc cgtagctcgg cgtggatgag accgtgaggc tctccgctga gcttggagcg
     6121 catgcccacg agcgagttga gcaggctgcg ctgctccttg agcgtgcggt acagcggcgg
     6181 gggctgctct tcgcgcggct tgctcgcggc acgacgtgac tggcgcgcct gccgctgctt
     6241 gagcaactcg cgcacttgat ccggctcgag gatgcccggc agcccgatga agtcgagctc
     6301 ctcatcgctt cccgtgagtg ccgcgaagcc gaactcgtcg ccgtcgaaca gcaccttgtc
     6361 gaagttggcg tcggactcga tggcctccca ggtgaactcg tcggtgagct catcggaggt
     6421 cgcctcctgc tggctctcgc gctccagcag ctcatcctcg agcagcttcg tgtcgtcgtg
     6481 ctcccggtcg agggcgtgat cgcgctccag ttcgagctgt gtcgccagtg acatcaggga
     6541 gggcacgctc ggcaggaaga tcgacgcggt ctccccccgg cggcgggcac gcacgaaccg
     6601 tccgacggcc tgagcgaagt acagcggagt cgacgccgac gtcgcataca cgccgaccgc
     6661 gagccggggc acgtcgaccc cctcggagac catcctcacg gccaccatcc agcggctctc
     6721 ccccgccgcg aacttctcga tgcgcgccga tgacgccttc tcgtcactga gcaccacagt
     6781 cgcgggttcg ccggtgatgt ccttcagcag ggcggcgtac gcgcgggctg cctgctggtc
     6841 ggtggcgatc acgaggccgc cggcatcagg gatggagtgc cgcacctcgg tgagacgggt
     6901 gttcgcggcg cgaagcacgg cgggcatcca ctcgcccttg gcatcgaggg cggtgcgcca
     6961 ggcctgagcg gtgatgtcct gagtgtcacc ctcgccgagc cgagcctcca tctcgtcgcc
     7021 catgcgcgtg cgccagcgca tctttcccgc gtaggcgaga aaaatcacgg gacgcactac
     7081 gccgtcggcc agcgcgcgcc cgtatccgta gctgtagtcg gtgagcgagg tgcggatgcc
     7141 cttctcgtcg gcgagatagc tcacgaaggg aatgggtgcc gtgtcgctgc gaaagggggt
     7201 tcccgtgagc gacaggcgcc gtgtcgcggg ctcgaaggcc tccctgatgg cgtcgcccca
     7261 gctgagcgcg tcgccgccgt ggtgcacctc gtcgaggatc acgagggtgc gcgctgactc
     7321 ggtgatctcc cggtgcaggg atgcccgaac cgccacctgc gcgtaggtga cggccatgcc
     7381 gtggaaccgc cgaccgccgt agccatcgcc gttcgcatag gcgggattga ggcgcaggcc
     7441 gaccttgtgc gccgcatccg cccactgccg cttgaggtgg tcggtgggcg cgaccacggt
     7501 gacccggtcg acgacccggc gggcgatgag ctcgacggcg agccgcagcg cgaacgtggt
     7561 cttgccggcg ccgggggtgg cggcggcgag gaaatccttg ggctcgcgat cgaagtaggc
     7621 gtcgagggcc tccgcctgcc aggcccgcag tttgtcggcg gtgcctcgag cagcgcgctc
     7681 ggggtatgac ggtgagaggt gctctgccgc gaaggttccg accgtggggg tcggaaagtc
     7741 cgtactgggc ggtggagtgc tcacttgatg gaacgttacc cgatgcgggt gacaccccgc
     7801 cccgcggtgg tcgggtctgt cgagactcag tcccgctgac cgagcctggg tcgctcggtg
     7861 gcagcgtggc cgatgaggcg agccggggtg acggctccgc gaccgaatcg cgcggacgcg
     7921 gcatccatca cgtcctcggc ctcgcgccag ccgtcggttt cgtcccacag gcccagctgc
     7981 acgtctgccg cgtcgagcag ttgttcggcg cggacgccga ccaggcgcac cggtcggtca
     8041 ttgccgagcg actcgtagat cgatcgcacc tcgtcgtaga tcgtgcggcc gaggtcggtg
     8101 ggttcgggca acgtgcgtga ccgggtgatc gtcgtgaagt cctcgtagcg cagcttgagc
     8161 accaccgtgc ggccgcgcat gcccgccttg cgcaggcgga cagccaccat gctcgacatc
     8221 c
//
LOCUS       DAOSOU010000080         8213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8687_length_8213_cov_7.383550, whole genome shotgun sequence.
ACCESSION   DAOSOU010000080 DAOSOU010000000
VERSION     DAOSOU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8687_length_8213_cov_7.383550"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLW15_05875"
     CDS             complement(<1..816)
                     /locus_tag="PLW15_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT33962.1"
                     /translation="MTARTRRGLFSALAIIVALAWVFPVYWMVNSAFLPNIILQSFIP
                     TFFPVKATLSNFTGALADGSFFSALGMSVAITLTVVVFCLLFAFLAAIAISRFRFRGR
                     RSFVIAVLIVQMLPAEGLFIAQYKMVASVGLLDSVLGVSVIYIAAVVPFTIWMLRGFV
                     AGIPADLEEAAMVDGLSRFGAFMRITFPLLAPGLVASGVYAFLQAWNEFTVALVLLPS
                     EAAQTLPLWLRGFVQASATRETDWGQVMAASTLVAVPVIIFFLIVQGRLTSGLV"
     gene            complement(813..1718)
                     /locus_tag="PLW15_05880"
     CDS             complement(813..1718)
                     /locus_tag="PLW15_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT33963.1"
                     /translation="MSTIAPRRGIRAPYVLIVPAVVILLAGMGYPLIWQFVTSMQEYG
                     LAQQFGKPAPFVGFDNFVTLATDPVLWTVVARSLAFCVLTAAVTLAVGTLLALLMTVV
                     TKTARLILQIALLLAWSMPVVAAMTVWIWLFDRRRGVVNYLLDMIPGVEMNRFDWLST
                     PLTFFAVASIIVIWMSVPFVAFSIYAGLTQVSDEVLEAAQLDGATGFQRFRHIIAPLI
                     RPVIAIVLLLQLIWDLRVFTQITMLQDAGSKSGDYDLLGTYIYKLGTSSQDFGMASAV
                     SILVLAITIALSWFYIRSLLKEDEA"
     gene            complement(1867..3156)
                     /locus_tag="PLW15_05885"
     CDS             complement(1867..3156)
                     /locus_tag="PLW15_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT33964.1"
                     /translation="MMKRKLGLVAAATAALVVLAGCSATPPADEGGKDITLWVMGGDT
                     PDALRDYLKTEYASATGGTLTIEQQDWSDALSKLTTALPDPENTPDVVEIGNTWSPTF
                     TSVGAFSDITDLVPELGGDKLLPSFVEIGKVDGANYALPYYFGSRYMFYRKDVWAAAG
                     LEVPTTLSEFSAAAAKLKTDTQSGFYIGGEDWRNGISWIFANGGDLAKKDGDTWKSTL
                     SDPKTIAGLEQLQALYADASLAPVTEADSTPWVNINNNAATGAPEAATIIAPGWAHWS
                     IGDLAPDPKDDTKTVATWNDDVFGTFVLPGVDGGAAPVFAGGSNIAISAASKNQSASR
                     ELMKIIFSADYQKMLGQNGLGPANLDYADSLGDDQFAKALVASAADSKLTPAAPGWAA
                     VEGSGLLEEFFGKVNGGGDIKALAAEYDTKIDALING"
     gene            complement(3225..4358)
                     /locus_tag="PLW15_05890"
     CDS             complement(3225..4358)
                     /locus_tag="PLW15_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOT33965.1"
                     /translation="MTTGFSTGRALRPTTKVLPEHARGHNRSLVLQTLYRAGQQSRAD
                     IARETGLTRVTVSDLVAELIVEGLIIETGQREDVRPGKPATLLDLNRGAFQIIGIDLS
                     EYATFRGAVLDLDGQILHRAELPLAGATGADATALVTALADTLVAAATLPVLGMGVGS
                     PGVVDLAGVVLSAPNLGWVDEPLQQAMSARFGVPVVVANDANVAALAEHSFGGADSDM
                     MLIKVGHGVGAGLLLDGTPLFGSRFAAGEIGHVVVGTDGGAECVCGKHGCLETWLAVP
                     RLEKAIAAAPDERTSILQQAGQRLGIALAPVVGALNLAEVVLSGPTSLLDGPLAQATI
                     DTLRNRTMAEFHGDLTLRMTTLGEDIVMRGAAVMVLSAQLGVS"
     gene            4547..5008
                     /locus_tag="PLW15_05895"
     CDS             4547..5008
                     /locus_tag="PLW15_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT33966.1"
                     /translation="MLAAPPSKVSIAVLAGLALTGALAGCSAAEDAGTDAGAGTSDSG
                     TSDSGTADTSAVYTDGTYTAEGSYQAPSGTESVDVTITLKGDVISSVEVVGNATDPQA
                     KQHQGEFIAGISDVVVGKDIDTIKVDKVGGSSLTSGGFNKAVEEIKADAAA"
     gene            5025..5858
                     /locus_tag="PLW15_05900"
     CDS             5025..5858
                     /locus_tag="PLW15_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT33967.1"
                     /translation="MGTGWQIDTAEPISTDLRAAIAARIETFDRAWSRFRDDSLVSRI
                     ARTPGSYRLPDEADELMGLYRGLYEATDAAVSPLVGRALEALGYDRAYSLRPSGPPAP
                     PPAWEDAIAWDGSTLTTVRPVLLDVGAAGKGLLVDIVTELLVGAGHTDLIVDGSGDIR
                     QVGPEPIRVGLEHPLDATKAIGIANVHNQAICSSATNRRAWGAGLHHVIDATTGLPTS
                     SVIATWAIAATAMLADGLSTALFFADPARLNERYDFTFARVFSTGRVEFSPHLDGELF
                     T"
     gene            5855..7381
                     /locus_tag="PLW15_05905"
     CDS             5855..7381
                     /locus_tag="PLW15_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOT33968.1"
                     /translation="MKQWLDRVTGIVTMYKLVIVALAGIGVVALVLSLFGIGTYSPLA
                     LLASAAVSIVATCSTSVLIARIMRVKPHLDSAMITALLIFFIMAPTLDPLGLVGIAIA
                     GLIANISKYLIAVRGRHVLNPAAVGVFVVALIAYTGFLGFGFAVWWLGSSTYLIPVVI
                     GAFLVLYRTQRLTIAAIFVALTTVGTVLYFLTVGETALSALQYVFLSSPVLFFAGFMF
                     SEPLTLPPRRWQQIVEAVVVALLFSVPFSFGPISRTPQLALLVGNLIGFAFGQRRGIR
                     LTYLGKTQLGPTTWELSFQPHRPVRFVPGQYMELTIPHRKADFRGSRRYFSISSAPAL
                     DAPITFAITTPSKSSSFKQALLDLEPGAAVTGTGVGGDFALPTDVTEPLLLVAGGIGI
                     TPFASQLAHATERGEKRDVVVLYSTSSPGPLPYGDLLARSGARVVLYAPEAPATLPPG
                     WEYAGAGRVTGERVASAVPDAAKRRAFVSGPPALVNDLRRALRSQGAKRVHSDYFSGY
                     "
     gene            complement(7308..>8213)
                     /locus_tag="PLW15_05910"
     CDS             complement(7308..>8213)
                     /locus_tag="PLW15_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT33969.1"
                     /translation="VASAERYSSHVLAQSLQDAASDRGIALQEAVDAHEVATHGIEAT
                     IGGQRVIVGKPAFVAERVSGLVRHEGASGEAAVYVGVGDAFAGVIVLRDEVRPESAET
                     LARLTRLGITNTMLITGDVEQTARPIADGLGITQMHAECLPEDKVNLVHDATPRPVIM
                     VGDGVNDAPVLAAADVGIALGARGSTAASESADVVILVDDLSRVADAVAIGHRTVNIA
                     LQSIWFGIAISVALMVVAAFGFIPAVLGAALQEVVDLITILGALRALSGGRGEARMAR
                     AISSPRSSPSAPASLPVNAARDGDR"
BASE COUNT         1349 a         2704 c         2838 g         1322 t
ORIGIN      
        1 gacgagaccc gaggtgagtc ggccctgcac gatgaggaag aagatgatca ccggcacggc
       61 gaccagcgtg gatgccgcca tcacctggcc ccagtcggtc tcgcgggtgg cgctcgcctg
      121 cacgaacccg cgcaaccaca gcggcagggt ctgcgccgcc tcggacggca gcagcacgag
      181 ggcgacggtg aactcgttcc acgcctgcag gaacgcgtag accccggatg cgacgaggcc
      241 aggagccaac agcgggaacg tgatgcgcat gaaggccccg aaccggctca agccgtcgac
      301 catcgcggcc tcctcgaggt cggcggggat gcctgcgacg aagccgcgga gcatccagat
      361 cgtgaagggc acgacggcgg cgatgtagat cacgctgacg ccgagcacgc tgtcgagcag
      421 cccgacgctc gcgaccatct tgtactgggc gatgaagagc ccctcggcgg ggagcatctg
      481 cacgatcagc acggcgatca cgaagctgcg ccgaccgcgg aaccgaaagc ggctgatcgc
      541 gatggcggcg aggaaggcga agagcaggca gaacacgacg accgtcagag tgatggccac
      601 gctcatgccg agggcgctga agaagctgcc gtcggcgagc gcgccggtga agttgctcag
      661 ggtcgccttc accgggaaga aggtggggat gaacgactgc aggatgatgt tgggcaggaa
      721 ggccgagttg accatccagt acacggggaa gacccaggcc agcgcgacga tgatggcgag
      781 cgccgagaac aggccgcggc gggtgcgggc ggtcatgcct cgtcctcctt gagcaggctg
      841 cgaatgtaga accagctcag cgcgatcgtg atggcgagca cgaggatcga gacggccgac
      901 gccatgccga agtcctgcga gctcgtgccg agcttgtaga tgtaggtgcc cagcaggtcg
      961 tagtcgcccg acttcgagcc ggcatcctgg agcatcgtga tctgggtgaa gacgcgcaga
     1021 tcccagatca gctgcagcag cagcacgatc gcgatgacgg ggcggatcag cggggcgatg
     1081 atgtgccgga accgctggaa gcccgtcgcg ccgtcgagct gggcggcctc gagcacctcg
     1141 tcggagacct gggtgagccc ggcgtagatg gagaaggcga cgaagggcac gctcatccag
     1201 atcacgatga tgctcgccac ggcgaagaag gtcaggggcg tcgagagcca gtcgaagcga
     1261 ttcatctcga caccggggat catgtcgagc agatagttga cgacaccgcg tcggcggtcg
     1321 aagagccaga tccagacggt catggcggcg acgaccggca tcgaccatgc cagcaggagg
     1381 gcgatctgca ggatgagcct ggccgtcttc gtgacgaccg tcatgagcag tgcgagcagg
     1441 gttcccaccg cgagcgtgac ggccgccgtc agcacgcaga acgcgagtga gcgggcgacg
     1501 acggtccaga gcaccgggtc ggtggccagg gtgacgaagt tgtcgaaccc gacgaacggg
     1561 gccggcttgc cgaactgctg ggcgagcccg tactcctgca tcgaggtgac gaactgccag
     1621 atcagcgggt agcccatgcc ggcgaggagg atcacgacgg cggggacgat gagcacgtac
     1681 ggggcacgga tgccgcggcg cggggcgatc gtggacatgg gggatcctcc ttccgggggt
     1741 gagtgagcgt gagggggtgg gtgacgctgg ttgagtagcg ggggcgcgag cccccgcgta
     1801 tcgaaacctc ggcctggcag cagggattcg atacggccct tcgggcctac tcaaccagcg
     1861 gtagtgctaa ccgttgatca gtgcgtcgat cttggtgtcg tactcagcgg cgagcgcctt
     1921 gatgtcgcca ccgccgttga ccttgccgaa gaactcctcg agcaggcccg aaccctcgac
     1981 tgcggcccag ccgggagcgg cgggggtcag cttcgagtcg gctgccgagg caaccagtgc
     2041 cttggcgaac tggtcgtcac cgagcgagtc ggcgtagtcc aggttggccg ggccgaggcc
     2101 gttctggccg agcatcttct ggtagtcggc gctgaagatg atcttcatca gctcacgcga
     2161 cgccgactgg ttcttgctgg cggccgagat ggcgatgttg gatccgccgg cgaagaccgg
     2221 agcggcacca ccatcgacgc cgggcagaac gaacgtgccg aacacgtcgt cgttccaggt
     2281 ggcgaccgtc ttggtgtcat ccttcgggtc gggagcgagg tcaccgatcg accagtgcgc
     2341 ccatccgggt gcgatgatcg tcgcggcctc gggcgcgccc gttgctgcgt tgttgttgat
     2401 gttcacccac ggggtgctgt ccgcctcggt gaccggggcg agcgaggcat ccgcgtagag
     2461 ggcctgaagc tgctcgagac ccgcgatcgt cttcgggtcg gagagcgtgg acttccacgt
     2521 gtcgccgtcc ttcttggcca ggtcgccgcc gttggcgaag atccacgaga tgccgttgcg
     2581 ccagtcctcg ccaccgatgt agaagccgga ctgggtgtcg gtcttgagct tcgccgccgc
     2641 tgcgctgaac tcgctcagcg tggtgggaac ctcaaggcca gccgcggccc agacgtcctt
     2701 gcggtagaac atgtagcgcg aaccgaagta gtagggcagt gcgtagttgg cgccgtccac
     2761 cttgccgatc tcgacgaacg agggcagcag cttgtcaccg ccgagctcgg gaacgagatc
     2821 ggtgatgtcg gagaaggcgc cgacgctcgt gaacgtgggc gaccacgtgt tgccgatctc
     2881 caccacgtcc ggagtattct cgggatcggg tagcgcggtg gtcagcttcg aaagggcatc
     2941 cgaccagtcc tgctgctcga tggtaagggt gcccccggtt gcacttgcgt actctgtctt
     3001 aaggtagtcg cgcagggcat ccggggtgtc acctcccatg acccagaggg tgatgtcctt
     3061 gccaccctcg tcagccgggg gtgtagcgga gcacccggcg agaacgacga gtgcggcggt
     3121 tgcggctgca accagtccga gctttcgctt catcatgtgt ctttcctttg ttgggggctg
     3181 cggggtgccc gggtgggaac tccgctggtg caggggtgaa agggtcagga gaccccgagt
     3241 tgcgcggaga gcaccatgac ggcagcgccg cgcatgacga tgtcctcccc gagcgtggtc
     3301 atccgtaacg tgagatcgcc gtggaattcg gccatcgtgc ggttgcggag cgtgtcgatg
     3361 gtcgcctgag ccagcgggcc atcaagtagt gaggtgggtc cgctcaggac cacttcggcg
     3421 aggttgagtg cgccgaccac gggagcaagc gcgatgccga gacgctgccc ggcctgctgc
     3481 aggatgctcg tgcgctcgtc aggcgcggcg gcgatcgcct tctcgagccg gggcacggcg
     3541 agccaggttt cgaggcagcc gtgcttgcca cacacgcact cggctccgcc gtcggtgccg
     3601 acgacgacgt ggccgatctc gcccgcggcg aacctgctgc cgaacagcgg ggttccgtcg
     3661 aggagcaggc cggcgccgac gccgtggccg accttgatga gcatcatgtc gctgtctgcg
     3721 ccgccgaagc tgtgctcggc gagggccgcg acgttggcgt cgttggcgac gacgacggga
     3781 acgccgaatc gcgccgacat cgcctgctgc aggggctcgt ccacccagcc gaggttgggg
     3841 gcgctcagca cgacgccagc gagatcgacg acaccgggtg atcccacccc catgccgagc
     3901 accggcaggg tggcggcggc cacgagagtg tcggcgagcg cggtgacgag ggccgttgca
     3961 tccgccccgg ttgccccggc gagcgggagt tctgcacggt gcaggatctg gccgtcaagg
     4021 tcgagcacgg cgccgcggaa ggtggcgtac tcgctgaggt cgatgccgat gatctggaag
     4081 gcgccacggt tgaggtcgag cagggtcgct ggcttgccgg ggcgcacgtc ctcgcgctgg
     4141 cccgtctcga tgatgaggcc ctcgacgatg agttcggcca cgaggtcgga gaccgtgacc
     4201 cgggtcagcc cggtctcgcg ggcgatgtca gcacgggact gctggccggc acggtacagc
     4261 gtctgcagca cgagcgagcg gttgtgtccg cgcgcgtgct ccggcagcac tttcgtcgtc
     4321 gggcgcaatg cgcgacctgt gctgaacccc gtcgtcatgt ttgttagtac accttacgaa
     4381 caaactaatc gcaagtcctt cgtccactgt ttaccgtgtt gttacattca agcgagtccc
     4441 tgcgttcaga cgtcgtgctg ggaattccct gcgatctccc acgatgcgtt cagcgcccgc
     4501 acgcaccatg cggcatgctg gtgacacgtc accgacagga gaaaccatgc tcgcagcacc
     4561 ccccagcaag gtatccatcg ccgtcctcgc ggggctggcc ctcacgggcg cactcgcggg
     4621 ctgttccgcg gcggaagacg cgggaaccga cgccggcgcc ggcacgagcg acagcggcac
     4681 ctctgacagc ggcaccgccg acaccagcgc ggtctacacc gacggcacct acaccgccga
     4741 gggcagctac caggcgccga gcggcacgga gagcgtcgac gtgacgatca cgctcaaggg
     4801 tgacgtgatc agctctgtcg aggtcgtggg aaatgccacc gacccccagg ccaagcagca
     4861 ccagggcgag ttcatcgcgg gcatctccga tgttgtcgtc ggcaaggaca tcgacacgat
     4921 caaggtcgac aaggtcggcg ggtcgtccct gaccagcggc ggattcaaca aggcagtcga
     4981 ggagatcaag gctgacgcgg ccgcatagcc tcactttcga ggccatcggc accggctggc
     5041 agatcgacac ggccgagccc atctcgactg accttcgggc agcgatcgcg gcgcgcatcg
     5101 agaccttcga tcgagcatgg tcgcggttcc gcgacgactc gctggtctcg cgcatcgcgc
     5161 gcacgcccgg cagctaccga ctccccgacg aggccgacga gctgatgggc ctctaccgcg
     5221 gcctgtacga ggctacggat gccgcggtca gccccctcgt cggccgcgcc ctcgaggcgc
     5281 tcggctacga ccgcgcctac tcactgcgcc cgagcggacc acccgcgccc ccgcccgcgt
     5341 gggaggacgc catcgcgtgg gacggctcga ctctcacgac cgtgagaccc gtgctgctcg
     5401 acgtcggtgc ggctggcaag ggtctgctcg tcgatatcgt gaccgagctg ctggtcggtg
     5461 ccgggcacac cgatctgatc gtcgacggca gcggtgacat ccggcaggtg ggccccgagc
     5521 cgatcagggt cgggctcgag catccgctgg atgcgacgaa ggcgatcggc atcgcgaacg
     5581 tgcacaacca ggcgatctgc tcgtcggcga ccaaccggcg cgcgtgggga gccgggctgc
     5641 accacgtgat cgacgcgacc acggggcttc ccacgagctc ggtcatcgcg acgtgggcga
     5701 tcgcagccac agccatgctc gccgacgggc tgtcgacggc actgttcttc gccgacccgg
     5761 cacgcctgaa tgagcgatac gatttcacct tcgcgagagt gttctcgacc ggtcgggtcg
     5821 agttctcgcc acacctcgac ggggagctgt tcacatgaag cagtggttgg atcgggtcac
     5881 aggcatcgtg acgatgtaca agctcgtgat cgtcgcactc gccggcatcg gagtcgtcgc
     5941 cctggtgctg tcgctgttcg gcatcggtac ctactcgccg ctcgccctgc tggcatccgc
     6001 ggccgtctcg atcgtcgcga cctgcagcac cagcgtgctg atcgccagga tcatgcgggt
     6061 caagccgcat ctggactcgg cgatgatcac agcgctcctg atcttcttca tcatggcgcc
     6121 gacgctcgat ccgctgggac tcgtcggcat cgccatcgcg ggcctgatag cgaacatctc
     6181 gaagtacctg atcgcggtgc gcggccgtca cgtgctcaac cccgctgccg tgggcgtctt
     6241 cgtggtggcg ctcatcgcgt acaccgggtt cctcggcttc ggattcgccg tgtggtggct
     6301 cggcagctcg acctacctga tccccgtggt gatcggagcc ttcctggtgc tctatcgcac
     6361 ccagcggctc acgatcgcgg ccatcttcgt cgcgctcacc acggtgggca ctgtgctcta
     6421 cttcctcacc gtcggcgaga ccgcactgtc ggcgctgcag tacgtgttcc tgtcatcacc
     6481 ggtgctgttc ttcgcgggct tcatgttcag cgagccgctc acgctgccgc cccgccgctg
     6541 gcagcagatc gtcgaagcgg tcgtggtcgc gctgctgttc agcgtgccgt tctcgttcgg
     6601 cccgatatcc cgcaccccgc agttggcact gctggtaggc aatctgatcg gcttcgcgtt
     6661 cggccagcgc cgcggcatcc ggctgacgta tctcggcaag acccagctcg ggcccacgac
     6721 gtgggagctg tcgttccagc cgcaccgacc ggtgcgattc gtgcccggcc agtacatgga
     6781 gctgacgatc ccccaccgca aggcggactt ccgcggcagt cgccggtatt tcagcatctc
     6841 gtcggcgccc gctctcgacg caccgatcac cttcgcgatc acgacaccgt cgaagtcgtc
     6901 gagtttcaag caggcgctgc tcgacctgga gcccggcgcc gccgtgacgg gcaccggagt
     6961 cggcggcgac ttcgccctac cgacggatgt caccgagcca ctgctgctcg tggccggcgg
     7021 catcggcatc accccgttcg ccagccagct cgcccacgcg accgagcgcg gggagaagcg
     7081 cgacgtcgtg gtgctgtact cgacgagctc ccccggcccg ctgccctacg gcgacctgct
     7141 cgccaggtcg ggcgctcgcg tcgtgctcta cgcgcccgaa gcgcccgcga ccctgccgcc
     7201 cgggtgggag tacgcggggg ctggtcgcgt caccggcgag cgtgtcgcct cagcggttcc
     7261 ggatgccgcg aagcgccggg cgttcgtctc cggtcccccg gcactcgtca acgatctccg
     7321 tcgcgcgctg cgttcacagg gagcgaagcg ggtgcactcg gactacttct cgggctactg
     7381 atcgcccggg ccatgcgagc ctctccccga ccgccgctga gcgcccgcag ggccccgaga
     7441 atcgtgatga ggtcgacgac ctcctggaga gccgcgccca gcacggcggg gatgaacccg
     7501 aacgccgcga caaccatgag cgccacgctg atcgcgatgc cgaaccagat gctctgaagc
     7561 gcgatgttca ccgtgcggtg accgatcgcc accgcgtcgg cgactcggct caggtcatcc
     7621 acgagaatca cgacgtcggc tgactcgctg gccgccgtcg atccgcgagc tccgagggcg
     7681 atgcccacgt cggccgccgc gagcaccggt gcgtcgttga caccgtcgcc gaccatgatc
     7741 acgggccggg gtgtggcgtc gtgaaccagg ttgaccttgt cttcggggag gcactcggcg
     7801 tgcatctggg tgatgccgag gccgtcggcg atgggtcgcg ccgtctgctc gacgtcgccg
     7861 gtgatgagca tcgtgttcgt gatgccgagg cgggtgagcc gggcgagcgt ctcggccgac
     7921 tcgggccgca cctcgtcgcg cagcacgatg accccggcga acgcgtcgcc caccccgaca
     7981 tacacggcgg cttcaccgct agcgccctcg tgccgcacca gcccgctgac ccgctcggcg
     8041 acgaaggccg gcttgcccac gatgacccgc tggccgccga tcgtcgcctc gattccgtgg
     8101 gtcgcgacct cgtgtgcgtc gacggcctcc tgcagcgcga ttccgcggtc gctcgcggca
     8161 tcctgcagcg actgcgcgag cacgtgcgac gagtaacgct cggccgacgc gac
//
LOCUS       DAOSOU010000081         8086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8878_length_8086_cov_6.907359, whole genome shotgun sequence.
ACCESSION   DAOSOU010000081 DAOSOU010000000
VERSION     DAOSOU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8878_length_8086_cov_6.907359"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PLW15_05915"
     CDS             complement(<1..970)
                     /locus_tag="PLW15_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HOT33970.1"
                     /translation="MHFGAVIQTHPPASRTVHLAKLAEQYGFEYVWTFDSHILWEEPY
                     VIYSAILAQTNRVIVGPMVTNPATRDWTVTASTFATLNEMYGNRTIIGIGRGDSAVRV
                     TNGKPTTLAELRESIHVIRELANSRSVEYNGAQLQLPWSKGSHVDVWVAAYGPLALKL
                     TGEVGDGFILQLADLDIAEWMIKSVRDAAEKAGRDPMAVKFCVAAPFYVGHDMEHMRN
                     QTRWFGGMVGNHVADIVAKYGEGSAVPKALTDYIKARQGYDYNEHGLAGNSHTDFVPD
                     EIVDRFCLLGTAEQHIEKLKALKELGVDQFAGYLQHDHKEETLRVYG"
     gene            complement(1070..2512)
                     /gene="hydA"
                     /locus_tag="PLW15_05920"
     CDS             complement(1070..2512)
                     /gene="hydA"
                     /locus_tag="PLW15_05920"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOT33971.1"
                     /translation="MSTTLITGGTVVTATGRSEADVLIDGETIVGVLNPGSVLLGSDL
                     ASSVDHVIDAKGKYVIPGGVDAHTHMELPFGGTAASDTFETGTRAAAWGGTTTIIDFA
                     VQTFGQKVMDGLAAWHAKAAGNCAIDYAFHQIIGEVNDDSLAAMKKLPDEGISSFKLF
                     MAYPGVFMSDDAQIFKAMQVSADTGMMTMMHAENGPVIDVIVKQLLAQGKTDPYYHGV
                     ARAWQMEEEATHRAIMLANLTGAPLYVVHVSAKQAVEQLAAARDIGQNVFGETCPQYL
                     YLSLEEQLGADSAEWGKFEGAKWVCSTPLRSKAEGHQHHMWQSLRTNDIQMVSTDHCP
                     FCMKGQKDLGLGDFSKIPNGIGSVEHRMDLLYQGVVDGQISLERWVEITSTTPARMFG
                     LYGKKGVIQPGADADIVIYDPQGHTSIGYGEGKTHHMNMDHSAWEGFEIDGHVDTVLS
                     RGKVVVDNNEYLGAKGDGKFVKRGLSQYLI"
     gene            complement(2605..3453)
                     /locus_tag="PLW15_05925"
     CDS             complement(2605..3453)
                     /locus_tag="PLW15_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT33972.1"
                     /translation="MTVVRAALTQTTWTGDKESMLAKHEQFARDAAAQGAQVICFQEL
                     FYGPYFGITEDKKYYAYAEPADGPIVQRFAKLAKELNLVMVLPIYEEDMPGVYYNTAV
                     MVDADGTILGKYRKHHIPHLDRFWEKFYFRPGNMGYPVFNTAVGPIGLYICYDRHFPE
                     GWRELGLNGAQIVFNPNATKPGLSNRLWEVEGPAAAVANGYFVLQPNRVGREDNEYGD
                     LAVDFYGTSQVIDPRGNFVGERGSGDSEELLIRDLDMDMIRQMRDDWQFYRDRRPESY
                     TSIPKP"
     gene            3561..4883
                     /locus_tag="PLW15_05930"
     CDS             3561..4883
                     /locus_tag="PLW15_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT33973.1"
                     /translation="MKSVALAIEEPTPRGIAASIARMITSGDLAPGDRLPTVRALAAD
                     LGVSPATVSHAWQTLSANGLIISRGRSGSVVRAVPKDWLPLRYRGMDGDLDARLDLSR
                     GTPDPELLPDLMASLARLSGRADTTSYLQKPDIPELHDLLRETWPAPVEALTVTNGAL
                     DAIDRVLARITRFGDRVIVGNPTFAPFFDLLDHYGLEALPVDLDDEGMIPEHFLAALG
                     HAPAVILLQNRAHNPSGISMTAARAEKLAWMLHRSRTAPNTIVVEDDHSGAISTAPLV
                     SLAAWMPDRVLHVRSYSKTHGPDLRIGALGGPRALVEQIISHRLLGPGWTSRMVQSIL
                     FDLLTNPEAVQRVSTARDVYAQRRATFSAAMAREGIMLPIGDGINAWVPVADERAAIV
                     SLAAAGIRVAPGSPFQLGGGAPHLRVTVGRISGDVDSIAAALAVAARA"
     gene            5073..6050
                     /locus_tag="PLW15_05935"
     CDS             5073..6050
                     /locus_tag="PLW15_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative F420-0 ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT33974.1"
                     /translation="MSRLHRAVPIVLAAVLALTGCATASAPTPSPTIDVAAPVTVDNC
                     GVTVTFDTAPERVVTIKSSTTELLLALGLGDRIVGTAFQDGPVPAEWADAAASIPSIS
                     DFVPNEEAVLQLSPDLVFGGWESNFASDGVGERAELESLGVNTYVAPSACRSIQPPKL
                     TWDDVFGDISEVASIFRVDASALVADQQARVDALLADKSGQGRTALWYSSGDDTPYVG
                     AGLGAPELVLETLGLTNIAADVDATWAPLNWEVVVDADPDVIVLVDATWNTAKDKIAM
                     LEANPATQNLSAVKAGRYLIVPFAATEAGVRSVEATESLRDQLAVIDGS"
     gene            6040..7131
                     /locus_tag="PLW15_05940"
     CDS             6040..7131
                     /locus_tag="PLW15_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOT33975.1"
                     /translation="MGRDVEGAGQAGSRQAGSRQARSPGGARSRVPLGLWAAGLGLLL
                     VASVTLAVTIGPADIRFDEVWASILSHLGIGTNPLPDLRDGIVWELRLPRILTAAAVG
                     AGLALSGAVMQAITRNPLADPYLLGLSSGASLGAVSVLLLGLAVLLPLAAFLGSMLAL
                     GLTLLLASSLGRITPTRTVLAGLAVSALASAITSLVIFWAVTGDSYREILGWLLGSLA
                     GARWPAVAISFGAILIAGVPIMLTGKLLDAFAFGDVSAASLGVNVSATRWGLLGASAL
                     LTGAMVSVSGSIGFVGLVLPHAVRLLVGSGHRALLPLSALVGGIFLVWADTLARTVFD
                     PRELPVGIVTALIGAPVFAVLLARRRGLT"
     gene            7128..7901
                     /locus_tag="PLW15_05945"
     CDS             7128..7901
                     /locus_tag="PLW15_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT33976.1"
                     /translation="MSGLEGSRITVSAGGALLIDGVSVDAPDGTLTALVGPNGAGKST
                     LLRALAAVQRPREGAVTFDGDDLLGMPRRQRARLAALVEQDAATDLAMPVTSVVELGR
                     LPHQSLWAVDPADTIVTGALDAVGMTDFAARDFGTLSGGERQRVMLARALAQQPRLLL
                     LDEPTNHLDIGAQLAVLGLLRKLAGDGMTVVAALHDLGLAATYADNVIVLRGGRVVAA
                     GATVDTLTPDLIREVYGVEAVVLHNPVTGRPVIALSPLG"
BASE COUNT         1145 a         2809 c         2740 g         1392 t
ORIGIN      
        1 cgccgtacac ccgcagcgtc tcctccttgt ggtcgtgctg gaggtaaccg gcgaactggt
       61 cgaccccgag ctccttgaga gccttgagct tctcgatgtg ctgctcagcc gtgcccagca
      121 ggcagaagcg gtcgacgatc tcgtcgggca cgaagtcggt gtggctgttg cccgcgagtc
      181 cgtgctcgtt gtagtcgtag ccttggcgag ccttgatgta gtcggtgagt gccttgggca
      241 ctgccgaacc ctcgccgtac ttggcgacga tgtcggcgac gtggttgccc accatcccgc
      301 cgaaccatcg ggtctggttg cgcatgtgct ccatgtcgtg accgacgtag aagggggctg
      361 cgacgcagaa cttcacggcc atggggtcgc gccccgcctt ctccgcggca tcccgaaccg
      421 acttgatcat ccactcggcg atgtcgaggt cggccaattg caggatgaac ccgtcgccga
      481 cctcgccggt cagcttgagc gctagcgggc cataggcggc cacccagacg tcgacgtggg
      541 agcccttgct ccacggaagc tggagctgcg cgccgttgta ctcgaccgag cgcgagttcg
      601 cgagctcccg gatcacgtgg atcgactcgc gcagctcggc gagcgtcgtc ggcttgccgt
      661 tggtgacgcg caccgccgag tcgccacggc cgatgccgat gatcgtgcgg ttgccgtaca
      721 tctcgttgag ggtcgcgaac gtggatgccg tcacggtcca gtcgcgggtc gccgggttgg
      781 tgaccatggg gcccacgatc acgcggttgg tctgggcgag gatcgcgctg tagatgacgt
      841 acggctcctc ccacaggatg tgggagtcga acgtccacac gtattcgaag ccgtactgct
      901 cggcgagctt ggcgaggtgc accgtgcgcg aggccggcgg gtgggtctgg atgactgctc
      961 cgaaatgcat gtactgctct ttctgttggt tgagtaggcc gggggccgta tcgaaaccgg
     1021 tggcggctgg tctcgataca cgcttcgcgc tactcgaccg gccggggtgc tagatgaggt
     1081 actgcgagag gccgcgcttg acgaacttgc cgtcgccctt cgcgcccagg tactcgttgt
     1141 tgtcgaccac gaccttgccg cgcgagagca cggtgtcgac gtgtccgtcg atctcgaagc
     1201 cctcccacgc cgagtggtcc atgttcatgt ggtgggtctt gccctcgccg tagccgatcg
     1261 acgtgtggcc ctgcgggtcg tagatgacga tgtcggcatc cgcccccggc tggatgacac
     1321 ccttcttgcc gtagagcccg aacatgcgcg ccggggtggt cgaggtgatc tccacccagc
     1381 gctcgagcga gatctggccg tcgacgacgc cctggtagag gaggtccatg cggtgctcga
     1441 ccgaaccgat gccgttaggg atcttcgaga agtcgccgag accgagatcc ttctgaccct
     1501 tcatgcagaa cgggcagtga tcggtcgaga ccatctggat gtcgttggtg cgcagcgact
     1561 gccacatgtg gtgctggtgc ccctcggcct tcgatcgcag gggggttgag cacacccact
     1621 tcgcgccctc gaactttccc cactccgcgc tgtcggcacc gagctgctcc tcgagcgaga
     1681 ggtacaggta ctgcgggcag gtctcgccga agacgttctg gccgatgtca cgggccgcgg
     1741 cgagctgctc gacggcctgc ttcgccgaga cgtgcacgac gtagagcggg gctcccgtga
     1801 gattggcgag catgatcgcg cggtgagtgg cctcctcctc catctgccag gcgcgggcga
     1861 cgccgtggta gtaggggtcg gtcttgccct gcgcgagcag ctgcttgacg atcacgtcga
     1921 tgacggggcc gttctcggcg tgcatcatcg tcatcatgcc ggtgtcggcc gagacctgca
     1981 tcgccttgaa gatctgcgcg tcatccgaca tgaacacacc cgggtaagcc atgaacagct
     2041 tgaagctgga gatgccctcg tcgggcagct tcttcatggc cgcgagcgag tcgtcgttga
     2101 cctcgccgat gatctggtgg aacgcgtagt cgatcgcgca gttgccggcg gccttggcgt
     2161 gccaggcggc gaggccgtcc atgaccttct ggccgaacgt ctgcaccgcg aagtcgatga
     2221 tcgtcgtcgt gccaccccag gccgcggcac gggtgccggt ctcaaaggtg tcggatgccg
     2281 cggtgccgcc gaacggcagc tccatgtggg tgtgcgcatc gacgcccccg gggatcacgt
     2341 acttgccctt cgcgtcaatg acgtgatcca ccgaggacgc gaggtctgag cccaggagga
     2401 cggacccagg attgaggaca ccgacgatcg tctcgccgtc gatgagaacg tcggcctcgg
     2461 agcggccggt cgcggtgact acggtgccac cggtgatgag tgttgtgctc aagggggact
     2521 ccttctcttc gctggttgag taggccgaag gccgtatcga aacctggtct cgatacgccc
     2581 ctgcggggct actcgaccgg ccgactacgg cttggggatc gacgtgtagg actccggacg
     2641 acggtcgcgg tagaactgcc agtcgtcgcg catctggcgg atcatgtcca tgtcgaggtc
     2701 gcggatgagc agctcctccg agtcaccgga accgcgctcc cccacgaagt tgccgcgcgg
     2761 gtcgatgacc tggctcgtgc cgtagaagtc gacggcgagg tcgccgtact cgttgtcctc
     2821 gcggccgacg cggttgggct ggagcacgaa gtagccgttc gcgaccgcgg cggcgggacc
     2881 ttcgacctcc cagagccggt tggagagccc cggcttggtg gcgttcgggt tgaagacgat
     2941 ctgggcgccg ttgaggccga gctcgcgcca tccctcgggg aagtgccggt cgtagcagat
     3001 gtacagaccg atgggtccga cggccgtgtt gaacacgggg tagcccatgt ttcccgggcg
     3061 gaagtagaac ttctcccaga agcgatcgag gtgcgggatg tggtgcttgc ggtacttgcc
     3121 gagaatggtg ccgtcagcat ccaccatcac ggccgtgttg tagtacacgc cgggcatgtc
     3181 ctcctcgtag atgggcagca ccatcacgag gttcagctcc ttggcgagct tggcgaagcg
     3241 ctgcacgatc gggccgtcgg ccggctccgc gtaggcgtag tacttcttgt cttcggtgat
     3301 gccgaagtag ggaccgtaga agagctcctg gaagcagatg acctgcgcgc cctgagccgc
     3361 cgcgtcgcgc gcgaactgct cgtgtttggc gagcatggac tccttgtcgc ccgtccacgt
     3421 cgtctgggtg agtgctgcgc gaaccacggt catctctgtg ctctcctcgc tggaaccttt
     3481 tgttattctg gggcctaaac atttcccgaa cgttacgttt cgtgacgttc ggcgtaaatc
     3541 tcgacactac ggcggttccc atgaagtcgg tagccctcgc gatcgaggaa cccacacccc
     3601 ggggcatcgc cgcgtcgatc gcgcgaatga tcacgagcgg cgacctcgcg ccgggggatc
     3661 gcctgccgac cgtgcgcgcg ctggcggccg acctcggtgt cagcccggcc accgtcagcc
     3721 acgcgtggca gacgctgtcg gccaacgggc tcatcatcag ccgggggcgc agtgggagcg
     3781 tcgtgcgcgc cgtgcccaag gactggctgc cgctgcgcta ccgcggcatg gacggtgacc
     3841 tcgacgcgcg gctcgacctc tcccgaggca cccccgaccc cgaactgctg cccgacctga
     3901 tggcttccct cgcgcggttg tccggacggg ccgacaccac cagctatctg cagaagcccg
     3961 acatcccgga gctgcacgac ctgctgcgcg agacctggcc ggcgccggtt gaggcgctca
     4021 cggtgaccaa cggcgccctc gatgcgatcg accgggtgct cgcgcgaatc acgcgcttcg
     4081 gcgatcgggt gatcgtcggc aaccccacct ttgcgccgtt cttcgacctg ctcgaccact
     4141 atgggctgga ggcgctgccg gtcgacctcg acgacgaggg gatgatcccc gagcacttcc
     4201 tcgcggcact cggtcacgca cccgcggtca tacttctgca gaaccgggct cacaacccca
     4261 gcggcatctc aatgacggcg gcccgtgccg agaagttggc ctggatgctg caccgcagcc
     4321 gcaccgcgcc caacaccatc gtcgtcgagg acgaccactc cggggcgatc tccacagccc
     4381 cgctggtgag cctggccgcg tggatgcccg accgggtgct gcacgtgcgc agctactcga
     4441 agacccacgg acccgacctg cggatcggcg cgctcggcgg cccccgcgcg ctcgtcgagc
     4501 agatcatctc gcaccgactg ctgggacccg gctggacctc ccgcatggtg cagtcgattc
     4561 tcttcgacct gctcaccaac ccggaagccg ttcagcgcgt gtcgaccgcg cgggacgtgt
     4621 acgcccagcg ccgcgcaacc ttcagcgccg cgatggcgcg cgagggcatc atgctgccga
     4681 tcggcgacgg catcaacgcc tgggtgccgg tggccgacga gcgcgcggcg atcgtgagtc
     4741 tcgcggcggc gggcatcaga gttgcgcccg gttcgccgtt ccagttgggc ggcggcgcac
     4801 cgcacctgcg cgtcacggtg ggacgcatct ccggcgacgt cgactcgatc gcggcggcgc
     4861 tcgcggtggc ggcgcgggcg tgacgcggcg cgccgcgcgg cggcgatagg cctgacccgc
     4921 gccgccgcgc ggcggcgata ggcttgacgc acttcgaccc gagcgccgcg tatggcgtgg
     4981 ttcgtcgctc ctgacttggg gcggccggtc atccgcagcc tctcgtgctg cgtatccgac
     5041 ccagcccatg tcccctgccc aggaagcccg tcatgtcacg tctgcaccgc gcagttccga
     5101 tcgttctcgc cgccgtcctc gccctcaccg gatgcgccac cgcgagcgcg ccgaccccct
     5161 ctccgaccat cgatgtcgcc gctccggtca ccgtcgacaa ctgcggggtg acggtgacgt
     5221 tcgacaccgc acccgaacgc gtggtgacta tcaagagctc caccacggag ctgctgctgg
     5281 cgctgggact cggcgaccgc atcgtgggca cggcgttcca ggacggcccg gtgcccgctg
     5341 agtgggcgga tgcagctgcg agcatcccgt cgatctccga cttcgtgccc aacgaggagg
     5401 ccgtgctgca gctgtccccc gacctcgtgt tcggcggctg ggagtcgaac ttcgcctccg
     5461 acggcgtcgg cgaacgtgcg gagctcgagt cgctgggcgt gaacacgtac gtcgcgccgt
     5521 cggcgtgccg cagcatccag cccccgaagc tgacctggga tgacgtgttc ggcgacatca
     5581 gcgaggtcgc gtcgatcttc cgcgtcgacg cctccgccct cgtcgccgac cagcaggccc
     5641 gagtggatgc cctgctcgcc gacaagtcgg gccagggtcg caccgccctc tggtactcct
     5701 ccggcgacga caccccctac gtgggagcgg gactcggcgc gcccgaactc gtgctcgaga
     5761 ccctcggact gaccaacatc gcggccgacg tcgacgccac gtgggccccg ctgaactggg
     5821 aggtcgtcgt tgacgccgac cccgacgtga tcgtgctggt ggatgccacc tggaacaccg
     5881 cgaaggacaa gatcgcgatg ctcgaagcca acccggcgac gcagaacctc agcgccgtca
     5941 aggcgggccg gtacctgatc gtgccgttcg ccgcgaccga ggcgggcgtg cgctcggtcg
     6001 aggcgaccga gtcgctgcgc gaccagttgg cggtgatcga tgggtcgtga tgtcgagggg
     6061 gcggggcagg cgggatctcg acaagcggga tctcgacaag ctcgatcacc gggcggcgct
     6121 cgatcacggg ttccgctggg gctgtgggcg gcggggctcg ggctgctgct cgtcgcctcg
     6181 gtgacgctcg ccgtcacaat cgggcccgcc gacatccgct tcgatgaggt gtgggcgtcg
     6241 atcctcagcc atctcggcat cggcacgaat ccgctgcccg acctgcgtga cggaatcgtc
     6301 tgggagttgc ggctgccgcg catcctcacc gcggcggcgg ttggcgcggg gctcgccctg
     6361 agcggtgcgg tcatgcaggc catcacccgc aacccgctcg ccgaccccta cctgctcggc
     6421 ctgtcgagcg gcgcgtcgct cggcgcggtg tcggtgctcc tgctcggact cgcggtgctc
     6481 ctgccgctcg ccgcgtttct cgggtcgatg ctcgccctcg gactgaccct gctgctcgcc
     6541 agttcgctcg ggcgcatcac cccgacccgc accgtgctcg ccgggctcgc cgtgtcggcg
     6601 ctcgcctccg cgatcacgag cctcgtgatc ttctgggcgg tgaccggcga ctcctaccgc
     6661 gagatcctcg gctggctgct cggctccctc gccggcgccc gctggcccgc cgtcgcgatc
     6721 tcgttcggcg ccatcctcat cgccggcgtg cccatcatgc tcaccggcaa gctgctcgac
     6781 gccttcgcgt tcggcgacgt gtcggcggcg tcgctggggg tcaacgtgag cgcgacgcgg
     6841 tgggggctgc tcggggcatc cgccctgctg accggcgcga tggtgtcggt atcggggtcg
     6901 atcggattcg tggggctcgt gctcccgcac gccgtgcgcc tgctcgtcgg cagcgggcac
     6961 cgagcgcttc tgccgctttc ggcgctggtc ggcggcatct tcctcgtctg ggcggacacc
     7021 ctcgcgcgca ccgtcttcga cccgcgcgaa ctaccggtcg gaatcgtcac cgccctcatc
     7081 ggcgcgcccg tgttcgccgt gctgctcgcc cggcgccggg ggctcacgtg agcgggctgg
     7141 aggggtcgcg catcacagta agcgccggcg gagcgctgct catcgacggc gtctccgtcg
     7201 atgcacccga cggcactctc accgcactcg tcgggcccaa cggcgccggc aagtccaccc
     7261 tgctgcgggc gctcgcggcc gtgcagcgac cccgcgaggg cgcagtgacc ttcgacggcg
     7321 acgacctgct cgggatgccg cggcgccagc gcgcccgcct cgccgccctc gtcgaacagg
     7381 atgccgccac cgacctcgcc atgcccgtca cctcggtcgt cgagctcggc cgcctccccc
     7441 accagtcact ctgggctgtg gacccggccg acaccatcgt gaccggcgcg ctcgacgcgg
     7501 tcggcatgac cgacttcgcc gcccgcgact tcggcaccct ctccggcggc gaacgccagc
     7561 gcgtcatgct cgctcgggcg ctcgcgcaac agccgcgcct gctgctgctc gacgaaccca
     7621 ccaaccacct cgacatcggc gcccaactcg ccgtgctcgg cctgctgcgc aagctcgccg
     7681 gcgacgggat gaccgtggtc gccgccctgc acgacctcgg cctcgccgcc acctacgccg
     7741 acaacgtgat cgtgctgcgg ggcggccgtg tcgtcgccgc cggagcgaca gtcgacacgc
     7801 tgacgcccga cctcatccgc gaggtgtacg gggtcgaggc ggtcgtgctg cacaacccgg
     7861 tcacggggcg acccgtgatt gcgttgtcgc cgctggggta gcgggacagc gcggccgggt
     7921 ttcgatacgc cgcttcgcgg ctactcaacc agcgggagct tcacaggcgc ggtttcgata
     7981 cgccgcttcg cggctactca accagcggcg gggggcgacg cggcgcgctg cggcaaccac
     8041 gccgccacca gcgagcgcgg cagcactgcc gccgcgagcc gtgcgc
//
LOCUS       DAOSOU010000082         8045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_8936_length_8045_cov_6.922153, whole genome shotgun sequence.
ACCESSION   DAOSOU010000082 DAOSOU010000000
VERSION     DAOSOU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8936_length_8045_cov_6.922153"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLW15_05950"
     CDS             complement(<1..377)
                     /locus_tag="PLW15_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOT33977.1"
                     /translation="MSTPEYRIEHDTMGEVRVPANALYGAQTQRAVENFPISGAGLER
                     AQIIALARIKRAAAIVNGEMGVIDQGISDAIASAAEKLIAGEHHEHFPVDTYQTGSGT
                     SSNMNMNEVLAHVATEILGSPVHP"
     gene            complement(426..1022)
                     /locus_tag="PLW15_05955"
     CDS             complement(426..1022)
                     /locus_tag="PLW15_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOT33978.1"
                     /translation="MLRGNERFVAGTPEHPRQDVERRQELAFAQEPHAALFGCSDSRL
                     AAEIIFDKGIGDLFVVRNAGQIISDSVIGSLEYAVGVLGVPLIVVLGHDSCGAVLAAI
                     AAEGEDAPVLPPHIAGLISKITPAVNRVIKASGTAEHAEIDSQEVGREHLRDTIAELL
                     ESSELISDAVAAGTLAVVGANYRLLEGRAVPDVIVGAV"
     gene            complement(1058..1687)
                     /locus_tag="PLW15_05960"
     CDS             complement(1058..1687)
                     /locus_tag="PLW15_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HOT33979.1"
                     /translation="MAGKQPAIVAELGRPETPEETAARKAENSRKRRANQTAFNLIVA
                     TIASLGIVLFLVVVVVRPNPEPAPGIDYVTIAAQSQPEASAELIVPVLPDTWSANAAN
                     FGARNEVPTWYVGFITPSVQFVAFNQGIGANPTWQATTLDNAAETGTVTIDGLTWTVY
                     DQRDKSDTGNFAYSMATVVGESTILLHGTAPVSEFELLASSIASEVPAQ"
     gene            complement(1690..1905)
                     /locus_tag="PLW15_05965"
     CDS             complement(1690..1905)
                     /locus_tag="PLW15_05965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT33980.1"
                     /translation="MGNEKIDVSDLSYEQARDELMQVVAELEQGSSSLEESLTLWERG
                     EALAARCEEWLKGAKARLDAARAAATD"
     gene            complement(1916..3124)
                     /gene="xseA"
                     /locus_tag="PLW15_05970"
     CDS             complement(1916..3124)
                     /gene="xseA"
                     /locus_tag="PLW15_05970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT33981.1"
                     /translation="MTTDDAPSTVDAPWPVALLSTKIKGWIERLGTAWVEGEITQWGV
                     SGGNVYGKLKDLEADATVSFTVWSSTRAKLPADLKQGDRVVALVKPNYWVKGGTLTMQ
                     VFEMRHVGLGDLLERLERLRQKLKAEGLFDHKKPLPFLPGCIGLVTGKDSDAEKDVLR
                     NAQLRWPAVKFRVVHAAVQGDRAAAEVTAAIQVLDADPEVEVIIVARGGGDFQNLLVF
                     SDEALVRAAAACVTPLVSAIGHEADRPLLDEVADLRASTPTDAAKRVVPDVAEELARV
                     QQALGSLRLRITQRLSAEVDRLETLRSRPVLASSSWIVDVRADDLSRYVQRSRDLVER
                     VLERSHTRVSELTAQLRALSPQRTLARGYAIAQLPDGRAVRSVSEAPAGTELRLTVAD
                     GRISATVDGG"
     gene            3245..4294
                     /locus_tag="PLW15_05975"
     CDS             3245..4294
                     /locus_tag="PLW15_05975"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOT33982.1"
                     /translation="MPAQRNRLKDTPVAGTKRVLLAAPRGYCAGVDRAVIAVEKALER
                     YGAPVYVRKQIVHNIHVVTTLEKQGAIFVDEVEEVPEGAHVVFSAHGVSPAVVQGAAD
                     RNLQAIDATCPLVTKVHREATRFARDDYEILLIGHHGHEEVEGTMGHAPERTTLVNSP
                     ADVPNIVVNNPDHLVWLSQTTLSVDETMETVRLLRERFPNLQDPPSDDICYATQNRQV
                     AIKKVAATADLVIVVGSANSSNTVRLVDVALESGAKAAYRVDYASEIRQEWLDGVATV
                     GVTSGASVPEVLVQEVLDDLADAGYTDVSQVVTAEEDLMFSLPKELRTDAAGNPDERG
                     LGGRIRQLDWQRGND"
     gene            4287..4778
                     /locus_tag="PLW15_05980"
     CDS             4287..4778
                     /locus_tag="PLW15_05980"
                     /inference="COORDINATES: protein motif:HMM:NF040366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6264 family protein"
                     /protein_id="HOT33983.1"
                     /translation="MTEPTPDPRPEPRWGQYSDAPPVVAAPETLPPPTVAAQRPPAPT
                     SDVVLTVLLLGIGLVDVIRQFSSFANFASVMRESYIQLGYGEFTSDALANSMGVAQNV
                     VRVLLILIAIVWSARRIRQHQRAFWVPLAAGGAAALVLLVGILALMLIDPALAIYLNT
                     LTG"
     gene            4791..6017
                     /locus_tag="PLW15_05985"
     CDS             4791..6017
                     /locus_tag="PLW15_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33984.1"
                     /translation="MRLAVLPRDDAARVITGATARSAWAVNAANLALTIPVLVEFLVS
                     RDLTAALWAPLAIVVVMLGLAVVAYLRPKVWVVVTFLVAGAIGAFAYQLALISAVPTI
                     ESDAVFLLNRPAVSLVLVAVGATSWFIALAWIFCGFLVSLLVSVAVAVVTGMPLRPGW
                     GPVLVFLMYVFVFVTLAAIQSRARSRVPNFELLEQETRRLQVQENLRARVTAAVHDTL
                     LNDLSIIMNSPDQLDQRVIDRVRADLHTLTSAEWLHESSEVIRDDQDSELRNQLMMIM
                     SDLQWRGLTVQITGSGTGIYRLAPDVAAALLDAVRACLENVLRHSQTTVAEVDLAYSD
                     DEVTVIVTDEGVGFDPHGVPADRLGIRHSVVDRVEAVGGSARVWSSPDSGTSIVMRMP
                     VLEVVRHHEESTHGES"
     gene            6004..7146
                     /locus_tag="PLW15_05990"
     CDS             6004..7146
                     /locus_tag="PLW15_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33985.1"
                     /translation="MARADRRYFALSAQQVDPLSWFTGPLVPLAVAGIMVVAATPAIV
                     LTWGLSSMPVLQIVALLACVASPLLVHLATRPIRRPIGWGIASVALALSATGVIVSAL
                     GYASSDFTIELWWAPSSLALAIVSLGPYLPGRVLIAVGGTATIVVTTIALLVVYPTSG
                     AWGPVGSAVIVSAPAVLSLASMVTFSYGVVSTMLPLLQSPSRLMVAGREVRDEAAEQI
                     ERVTLAQLTARAVPFLESLADSGRVTPASRALAGQIARRLRDDLVTQGSVSWLDSIAQ
                     ESRLVVVDPERLARRMNSPQRTALRGLLEAVLAAPGTDTGSLMVELRKAPDGATAVAV
                     SLDMALPEGRRIMHLAPYYLTLKTAVDDLEVGRHGISFRIAPRDAP"
     gene            complement(7143..>8045)
                     /gene="fbaA"
                     /locus_tag="PLW15_05995"
     CDS             complement(7143..>8045)
                     /gene="fbaA"
                     /locus_tag="PLW15_05995"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT33986.1"
                     /translation="EAGSGGIIQVTTGGADYFAGQSVKARASGALAFAAFVTEVAKNY
                     PITVALHTDHCPKDALDGFVMPLIAASEAEVKAGRNPIFQSHMWDGSAVPLAENLEIA
                     KTLLPRMKAINAILEVEIGVVGGEEDGVSHDINEHLYTTLDDAIATVEALGLGEQGRY
                     MAALTFGNVHGVYKPGNVKLKPELLGTIQAGLQAKYGTSSAHPLDLVFHGGSGSSPEE
                     IATAVANGVVKMNIDTDTQYAFSRSIADTVLRNYDGFLKVDGEVGNKKIYDPRAWGKL
                     AETAMAARVVEATTELGSAGHSGN"
BASE COUNT         1197 a         2709 c         2723 g         1416 t
ORIGIN      
        1 gggtgcaccg gcgagccaag gatctcggtg gccacgtggg cgagcacctc attcatgttc
       61 atgttcgacg acgtgccact gccggtctgg tacgtgtcga cggggaaatg ctcgtggtgc
      121 tcgccggcga tcagcttctc ggcggcggat gcgatcgcat ccgagatgcc ctggtcgatc
      181 acgcccatct cgccgttgac gatggccgct gcgcgcttga tgcgcgccag agcgatgatc
      241 tgcgcgcgct caaggcccgc acccgagatg gggaagttct ccaccgcgcg ctgcgtctgt
      301 gcgccgtaga gggcgttggc gggaacccgc acctcgccca tcgtgtcgtg ctcgatacgg
      361 tattcggggg tgctcacgcc agtgtcgtcc ttctgtgtgc ctgcggggga gagaaagtgg
      421 tggagtcaga ccgcgcccac gatcacgtcg ggaaccgcgc ggccttcaag caggcgatag
      481 ttcgcgccca ccacagccag cgtacccgcg gcaaccgcgt cgctgatgag ttcggatgat
      541 tcgagcagct cggcgatcgt gtcgcgcagg tgctcgcggc cgacctcctg cgagtcgatc
      601 tcggcgtgct ccgcggtgcc ggaagccttg atgactcgat tcaccgccgg agtgatcttc
      661 gagatgaggc ccgcgatgtg cggcggcagc accggcgcat cctcaccctc ggcggcgatc
      721 gcggcgagca ccgctccgca gctgtcgtga ccgagcacga cgatcagcgg cacgccgagc
      781 acgccgaccg cgtactcgag agaaccgatg acggaatccg agatgatctg gccggcgttg
      841 cgcacgacga acaggtcgcc gattcccttg tcgaagatga tctcggcggc cagacgggag
      901 tcgctgcatc cgaacagggc ggcgtgcggc tcctgggcga acgcgagctc ctgacgacgc
      961 tcgacgtcct gacgggggtg ctcgggggtg ccggcgacga agcgctcgtt gccgcgcagc
     1021 atctcgcgcc agacatccgc tggggttcga ttggtcgtca ctgtgcaggt acctccgaag
     1081 cgatggatga cgccagcaac tcgaactcgc tgaccggagc cgtgccgtgc agcagaatcg
     1141 tgctctcgcc gaccacggtc gccatggagt acgcgaagtt gccggtgtcg gacttgtcgc
     1201 gctggtcgta cacggtccag gtgaggccgt cgatggtgac ggtgcccgtc tcggcggcgt
     1261 tgtcgagcgt cgtcgcctgc caggtcgggt tcgcgccgat gccctggttg aaggcaacga
     1321 actgcacgct cggggtgatg aagccgacgt accaggtggg cacctcgttg cgagcgccga
     1381 agttggcagc gttggcgctc caggtgtcgg ggagcaccgg aacgatgagc tcggccgatg
     1441 cctcgggctg tgactgggca gcgatcgtga cgtagtcgat gccgggcgct ggctcggggt
     1501 tcggccgcac cacgacgacc accaggaaca gcacgattcc gagggacgcg atcgtggcga
     1561 cgatcaggtt gaacgcggtc tggttggcgc gccgcttgcg cgagttctcg gccttgcggg
     1621 ccgccgtctc ctcgggggtc tccggtcgtc cgagctcagc gacgatcgcg ggctgcttgc
     1681 ctgccacggt cagtcggtcg ctgcggctcg tgccgcgtcg agacgggcct tggcaccctt
     1741 gagccactcc tcgcaccggg ccgcgagagc ctccccgcgc tcccagagag tcagcgactc
     1801 ctcgaggctc gatgatccct gctcgagctc tgcaacgacc tgcatgagct cgtcgcgcgc
     1861 ctgctcgtag ctgaggtcgc tcacgtcgat cttctcgttg cccacggtca cgattctatc
     1921 cgccgtccac tgtcgccgag atgcggccgt cggcgactgt gagtcgaagc tcggttcccg
     1981 ctggcgcctc cgataccgac cgcacggctc ggccgtcggg cagctgggcg atcgcgtatc
     2041 cgcgggcgag ggtgcgctgc ggcgacagcg cccgcagctg cgcggtcagt tccgacaccc
     2101 gggtgtggct gcgctcgagc acccgctcga cgaggtcccg gctgcgctgc acgtagcgcg
     2161 acagatcgtc ggcgcgcaca tccacgatcc agctggatga cgcgagcacc ggacgagatc
     2221 gcagcgtctc gagtcgatcc acctccgccg acagccgctg ggttatccgc aggcgcaggg
     2281 atccgagcgc ctgctgcacg cgggcgagct cctccgccac gtccggcacc acccgtttcg
     2341 ccgcatccgt gggggtggat gcccgcaggt cggcgacctc gtcgagcagc ggtcgatcgg
     2401 cctcatgccc gatcgcggac accaagggag tcacgcaggc ggcagccgcc cgcacgagtg
     2461 cttcgtcgct gaacacgagg aggttctgga agtcgccgcc gccgcgggcg acgatgatga
     2521 cctcgacctc gggatcggcg tcgagcacct ggatcgccgc ggtcacctcg gcggccgccc
     2581 ggtcaccctg caccgcggcg tgaacgaccc ggaacttcac cgcgggccag cgcagttgcg
     2641 cgttgcgcag tacatccttc tccgcgtcgc tgtccttacc ggtcacgagt ccgatgcacc
     2701 cgggcaggaa cggcaggggc ttcttgtggt cgaacaggcc ctcggccttg agcttctggc
     2761 gaaggcgctc gaggcgttcg agcaggtcac cgagcccgac atggcgcatc tcgaagacct
     2821 gcatcgtgag cgtgccaccc ttgacccagt agttcggctt gacgagcgcg accacgcggt
     2881 cgccctgctt gagatcggcg ggcagtttcg cgcgggtcga ggaccacacc gtgaaggaga
     2941 ccgtggcatc cgcctcgagg tctttcagct tgccgtagac gttgccgccg gagactcccc
     3001 actgggtgat ctcgccctcg acccaggccg tgcccagccg ctcgatccag cccttgatct
     3061 tggtggagag cagggctacc ggccacggtg cgtcaacggt ggatggtgcg tcatccgtcg
     3121 tcatcgtcgc cctcccgaat cgctggtcag catggttcgg cccgtactgt tcggcccgcc
     3181 gcctagaatt ggcagtgtga gcaccatctc gaacgggagc gtcacggccc tcgaaacgcc
     3241 gcgcatgccc gcccaacgga acaggctaaa ggatacccct gtcgccggaa ccaagcgggt
     3301 gctcctcgct gcgccccggg gatactgcgc gggtgtggat cgtgccgtga tcgccgtgga
     3361 gaaagcactg gagcgctacg gtgccccggt gtatgtgcgc aagcagatcg tgcacaacat
     3421 ccacgtggtg accacgctgg agaagcaggg cgccatcttc gtcgacgagg tggaggaggt
     3481 ccccgaggga gctcacgtcg tcttcagcgc tcacggcgtc tcgcccgccg tggtgcaggg
     3541 tgctgccgac cgcaacctcc aggcgatcga cgcgacctgt ccgctcgtca ccaaggtgca
     3601 ccgcgaggcc accaggttcg cccgcgatga ctacgagatc cttctcatcg gccaccacgg
     3661 tcacgaggag gtcgagggca ccatgggtca cgcccccgag cgcacgaccc tcgtcaacag
     3721 ccccgccgat gttccgaaca tcgtcgtgaa taaccccgac cacctggtct ggctctcgca
     3781 gacgaccctg agcgtcgacg agaccatgga gactgtgcgg ctgctgcgcg agcgcttccc
     3841 gaacctgcag gacccgccga gcgacgacat ctgctacgcc acccagaacc gccaggtcgc
     3901 gatcaagaag gtcgcggcca ccgccgatct cgtgattgtc gtcggtagcg ccaacagctc
     3961 caacacggtg cggctcgtgg atgtcgcgct cgagagcggc gcgaaggccg cctaccgcgt
     4021 cgactacgcg agcgagatca ggcaggagtg gctcgacggc gtcgcgacgg tcggcgtcac
     4081 gagcggggca tccgtgccgg aggttctcgt gcaggaggta ctcgacgacc tcgccgacgc
     4141 gggttacacc gacgtgagcc aggtggtcac cgccgaggag gacctcatgt tctccctgcc
     4201 gaaggaactg cgcaccgacg ccgcgggcaa tcccgatgag cgcgggctcg gcgggcgcat
     4261 ccgtcagctg gactggcagc gcggcaatga ctgaaccgac gcccgatcct cgccccgaac
     4321 cgcgctgggg ccagtactct gacgccccgc cggtcgtggc cgcgcccgaa acgcttccgc
     4381 cgcccaccgt cgcggcgcag cgcccgcccg cgccgacctc cgatgtcgtg ctcacagtgc
     4441 tcctgctcgg gatcggactc gtcgacgtca tcaggcagtt cagttcgttc gcgaactttg
     4501 cgagcgtgat gcgcgagtcg tacatccagc tcggttacgg cgagttcacc tcggacgctc
     4561 tggcgaactc catgggagtc gcacagaacg tcgtgcgcgt tctgctcatc ctgatcgcga
     4621 tcgtgtggtc tgcccggcgt atccgccagc accagcgcgc cttctgggtg ccgctcgcgg
     4681 cgggcggtgc tgccgcactg gtgctactcg tgggcatcct cgccctgatg ctcatcgatc
     4741 ccgcgctcgc catctacctg aacaccctga ccggatagcg cgggcagctg gtgcgactag
     4801 ccgtccttcc tcgggatgac gcggcccggg tcatcaccgg ggccacggcg agaagcgcct
     4861 gggcggtcaa cgctgccaac ctggcgctga ctatccccgt gctcgtcgag ttcctcgtga
     4921 gccgggatct gacggcggcg ctgtgggcgc ccctcgcgat cgtcgttgtc atgctcgggc
     4981 tcgcggtggt cgcgtacctc aggccgaagg tctgggtagt cgtgacgttt ctcgtggccg
     5041 gcgccatcgg cgcattcgcc taccagctcg cactcatctc agcggttccg accatcgaaa
     5101 gcgacgccgt gttcctgctg aaccggccgg cggtctccct cgtactggtg gcggtcgggg
     5161 cgaccagctg gttcatcgcc ctggcctgga tcttctgcgg ctttctggtc tccttgctgg
     5221 tgagcgtggc ggtagccgtg gtcaccggga tgccgcttcg gcccgggtgg gggcccgtcc
     5281 tggtcttcct catgtacgtc ttcgtgttcg tcaccctcgc ggccattcag tcccgggcgc
     5341 gcagccgggt gcccaacttc gaactactcg aacaggagac gcgtcggctc caggtgcagg
     5401 agaacctccg ggctcgggtg acagctgccg tgcacgacac gctcctgaac gatctctcga
     5461 tcatcatgaa ctcacccgac caactcgacc agcgggtcat cgatcgcgtt cgcgcggatc
     5521 tgcatacgct cacgagtgcc gagtggttgc acgagtcgtc cgaggtgatc cgtgacgacc
     5581 aggattccga gctgcgtaac cagctcatga tgatcatgag cgacctccag tggcggggac
     5641 tcaccgtgca gatcacgggc agcggaaccg gaatctatcg cctcgcccct gatgtcgcgg
     5701 ccgcgctgct ggatgcggtc cgggcgtgcc tggagaacgt gctgcggcac tcacagacga
     5761 ccgtcgcgga ggtcgacttg gcctattccg acgatgaggt gacagtcatc gtcaccgatg
     5821 agggagtcgg attcgacccg cacggggtcc ccgccgaccg gctgggcatc cgtcactccg
     5881 tcgtcgaccg cgtcgaggcc gttggcgggt cggcgcgcgt gtggtcgtcc cctgattcgg
     5941 gcacgtcgat agtcatgcgg atgcccgtgc tcgaggtcgt gcgacaccat gaggagtcca
     6001 cccatggcga gagctgaccg acggtacttc gcgctctccg cgcaacaggt ggatccgctg
     6061 agctggttca ctgggccgct ggtgccgctc gcagtggcgg gcatcatggt ggtcgctgcc
     6121 actcccgcga tcgtgctgac gtgggggctg tcgagcatgc cggtgctcca gatcgtcgcg
     6181 ctgctcgcgt gcgtggcatc cccgctgctg gttcatctgg ccacccggcc gattcgtcga
     6241 ccgatcggat ggggtatcgc ctcggtcgcg ctcgcgctgt cggcgaccgg agtcattgtc
     6301 tcggccctcg gctacgcgag cagcgacttc accatagaac tgtggtgggc gccgagctcc
     6361 ttagcccttg ccatcgtcag cctcgggccg tacctgcccg gccgggtact gatcgctgtc
     6421 ggtggaactg cgacgattgt ggtcaccacc attgcgctcc tcgtcgtgta tcccacatcc
     6481 ggtgcgtggg ggccggtcgg atcggccgtg atcgtctcag cgccagcggt gctctccctc
     6541 gcgtcgatgg tgaccttctc ctacggggtt gtcagtacga tgctcccgct gctgcagagc
     6601 ccctcacggc tgatggtcgc cgggcgcgag gtgcgcgacg aggcggccga gcagatcgag
     6661 cgggtgaccc ttgcgcagct gaccgctcgt gcggtgccct tcctggaatc gctcgccgac
     6721 tcgggacgcg tgactcctgc gagccgcgct ctggcgggcc agatcgcgcg gcggctgcgc
     6781 gatgacctgg tcactcaggg gtcggtgagc tggcttgatt cgattgcgca ggaatcccgg
     6841 ctcgtcgtcg tcgacccgga gcgcctcgcg cgccgcatga acagtccgca gcgcaccgcg
     6901 ctgcgtgggc tgctggaggc ggtacttgcc gcccccggca ccgacaccgg gtcactgatg
     6961 gtcgagttgc gcaaggcgcc cgacggagcg acagccgtcg cggtgagcct cgacatggcg
     7021 ctgcccgagg gcaggcgcat catgcacctg gcgccgtact acctcacgct gaagacggcc
     7081 gtcgacgatc tcgaggtggg gcgacacgga atctcattcc ggatcgcccc tcgcgacgcg
     7141 ccctagttgc cggagtggcc ggccgagccg agttcggtcg tggcttcgac cacccgagcg
     7201 gccatggccg tctcggcgag cttgccccag gcgcgagggt cgtagatctt cttgttgccg
     7261 acctcgccgt cgaccttcag gaaaccgtcg tagttgcgca gaacggtgtc ggcgatcgag
     7321 cggctgaagg cgtactgggt gtcggtgtcg atgttcatct tcaccacgcc gttcgcgacc
     7381 gccgtggcga tctcctcggg gctcgagccg gagccaccgt ggaagaccag gtcgagaggg
     7441 tgggccgagg aggttccgta cttggcctgg agcccggcct ggatggtgcc cagcagctcc
     7501 ggcttgagct tgacgttgcc gggcttgtag acgccgtgca cgttgccgaa tgtgagggcc
     7561 gccatgtacc gaccctgctc gccgaggccg agggcctcga cggtggcgat cgcgtcatcc
     7621 agagtcgtgt agaggtgctc gttgatgtcg tggctgacgc cgtcctcctc gccaccgacg
     7681 acgccgatct cgacctcgag gatcgcgttg atcgccttca tgcgcggtag cagggtcttc
     7741 gcgatctcca ggttctcggc gagaggtacc gccgagccgt cccacatgtg ggactggaag
     7801 atcgggttgc gccccgcctt cacctcggcc tcggatgcgg cgatgagggg catgacgaag
     7861 ccgtcgagtg catccttcgg gcagtggtcg gtgtggaggg cgaccgtgat cgggtagttc
     7921 ttggcgacct cggtcacgaa cgcggcgaac gcgagagctc cggatgcgcg cgccttgacg
     7981 ctctgcccgg cgaagtagtc ggcgccaccg gtcgtcacct ggatgatgcc acccgaaccc
     8041 gcctc
//
LOCUS       DAOSOU010000083         7971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_9050_length_7971_cov_7.276529, whole genome shotgun sequence.
ACCESSION   DAOSOU010000083 DAOSOU010000000
VERSION     DAOSOU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9050_length_7971_cov_7.276529"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="PLW15_06000"
     CDS             <1..2278
                     /locus_tag="PLW15_06000"
                     /inference="COORDINATES: protein motif:HMM:NF004800.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOT33987.1"
                     /translation="DNLMRSATAHAERLLDGVIDFGDVMLSWRVAELACTISSILHHE
                     GASPLSVLPAIASFHRIRPLSMPEIEALWPLVVIRGAVLVASGSQQVRNDESNDYARE
                     GLVREESIFHSASPVPSPVMTRIIAQHLGLTSSELAPKPAASRLIAGDHSVALLDLGV
                     GSRILDEGAWVTDSSARRAVADAVDSLFARGNDLVIAPFGAIDASRSRPLSVDPASTW
                     RTGTDVWVATEVPLVAPWAGELTVAPSGLVLTGEDATLRITADEGMPGSEPTSVSLGA
                     PLGVLRPGTSYRLTVVTNDLESDVPAFAPPAYAAGWMAYLLDPGAILGIGDAAAPSTG
                     GSGELMERREAVLADVQEHYYAQPPRIERGWKHHLMGDDGRVYLDMVNNVTVLGHSHP
                     RVARAVADQWRTFNSNSRFHYGSIVALAEKLTALLPESLDTVFLVNSGSEAVELALRL
                     ARIHTKRDDLLAVRESYHGWTYLADAVSTSAADNPNALASRPEWVHTLDVPNTFRGRY
                     REDEAHRYAQDATAAVRELVASGRPPAAFICEPVYGSAGGVTLPEGYLEAVYSEVRRS
                     GGVTIADEVQVGLGRLGSWFWGFEQQDVVPDIVTIAKSLGDGHPLGAVVTSKAIAASY
                     AAEGYFFSSTGGSPVSSIVGATVLDVIAEEDLAGNARRVGGYLKGRLQELALRHSLIG
                     TVHGEGLYLGVEFVRDRVSLEPATAETSAICDRLRQLGVIVQPTGNFMNVLKIKPPLS
                     FDEDAADFFVDTLDRVLA"
     gene            2458..2910
                     /locus_tag="PLW15_06005"
     CDS             2458..2910
                     /locus_tag="PLW15_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOT33988.1"
                     /translation="MTHPASNTIWLTRGARDRLEAELAELSHGAAPSPAVEGRIRELL
                     ALLRRTEVGDKPDDGLVEAGMVVTVQFEGDRDATTFLLAHRGVTVTDSTIEIDIYSPE
                     SPLGSAIANKYPGDSFHYETPTGSHVRGTVVSATPFKESVPAAPAISN"
     gene            complement(2907..4223)
                     /gene="gdhA"
                     /locus_tag="PLW15_06010"
     CDS             complement(2907..4223)
                     /gene="gdhA"
                     /locus_tag="PLW15_06010"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOT33989.1"
                     /translation="MFDEVIARNPGEIEFHQAVREVLESLGPVVAKHPEYADSEVIRR
                     LCEPERQIIFRVPWTDDRARVQLNRGFRVEFNSALGPYKGGLRFHPSVYLGIVKFLGF
                     EQIFKNSLSGLPIGGAKGGSDFDPKGRSEAEVMRFCQSFMTELYRHLGEYTDVPAGDI
                     GVGGREIGYMFGQYKRITNRYESGTFTGKGLSWGGSQVRTEATGYGTAFFVKEMLKAS
                     AESFDGKRVVVSGSGNVAIYAIEKVHQFGARVVACSDSSGYVVDEAGIDLDLLKEIKI
                     ERRGRIDEYADRRASASFISDGSIWDVPCDVALPCATQNELDDENAIRLIKNGCRVVA
                     EGANMPTTPRAIHTLRDAGVRFGPGKAVNAGGVATSALEMQQNASRDSWTFDYTEERL
                     SEIMTSVHHRCLETADEYGAPNDYVVGANIAGFTRVADAMLALGVI"
     gene            complement(4558..4633)
                     /locus_tag="PLW15_06015"
     tRNA            complement(4558..4633)
                     /locus_tag="PLW15_06015"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4598..4600),aa:Phe,seq:gaa)
     gene            complement(4697..4770)
                     /locus_tag="PLW15_06020"
     tRNA            complement(4697..4770)
                     /locus_tag="PLW15_06020"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4734..4736),aa:Asp,seq:gtc)
     gene            complement(4791..4866)
                     /locus_tag="PLW15_06025"
     tRNA            complement(4791..4866)
                     /locus_tag="PLW15_06025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4830..4832),aa:Glu,seq:ttc)
     gene            5144..6367
                     /locus_tag="PLW15_06030"
     CDS             5144..6367
                     /locus_tag="PLW15_06030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT33990.1"
                     /translation="MTSRTAALPSGMRGRRLAAILAVLATFATGTIIASAPAYATDYP
                     TWSDVAAVRNDESATANKIAEIEGMLAGLQAEAERTQKDAEAKGVIWGIADAKFQEAA
                     YRADNLKKQADAAAATALASGQRAGQWAAQLARSGGGDITTDLFANTGDADNLLYGLG
                     MSSKISDQAYSIYERALLDRNTAQALTDQADVAKNELEALKLVAEKAFNEAQASADAA
                     GKALAEQQDNQARLQQQLIVLKEKRAATEADYLAGVRERIGSGASLDAGEISLSGWAR
                     PTAGRITDGYGPRVSPLPGASSFHQGTDIAASCGQGIYAASSGTVVYAGWNGTYGNFV
                     MIDHGGGITTGYAHIVSGGILVSYGQEVVVGQQIASVGSTGLSTGCHLHFEVRINGYS
                     TDSVPFMAGQGITIG"
     gene            6395..7597
                     /locus_tag="PLW15_06035"
     CDS             6395..7597
                     /locus_tag="PLW15_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOT33991.1"
                     /translation="MSRHFRPMGALATVVVGAVLVAVGIVGPVAAEDYPSWDDVQAAR
                     QNEADTQAAIDEIEGLLVGLETEAADLGRTAQVKAEAYNAANSALEDATAAADALARQ
                     SKDAAAKATESSQRAGQLIAQLARTGGGSLTLELLMSSNADDLLGSLGTLTKVSAQAG
                     EIYRQAVIDKNLAGSLAEQAKVAQTKRAELAATAKSAYTEAQSASDAALARLAEKQAA
                     SDQLYAQLASLKGTTSSVEQGYLEGLTAEQEGQNQPPPPDSGPAPNPPPPAPNGSAVA
                     GALSFAYAQLGDMYQFAGSGPDVWDCSGLTRGAYASVGVYIGTHSATNQYWTMAYAGR
                     LVPLSDMVAGDLIFYADNGDISNSAYHVTMYVGGGQMIEAPYDGVPVRVVNVRYYDIV
                     PYAGRPTP"
     gene            complement(7686..>7971)
                     /locus_tag="PLW15_06040"
     CDS             complement(7686..>7971)
                     /locus_tag="PLW15_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOT33992.1"
                     /translation="GVTISVRPVAVFNMTDDAGIDSKVICVQAKDPRWSHIQDLDDVP
                     EYTRKEIEHFFEHYKDLEPGKWVKVEGWEGAARAEEIVLAGFEKLKAEGH"
BASE COUNT         1373 a         2720 c         2511 g         1367 t
ORIGIN      
        1 cgacaacctg atgcggtccg cgacggcgca cgccgagaga ctcctcgacg gggtgatcga
       61 cttcggcgac gtgatgctct cctggcgggt ggccgagctc gcgtgcacga tctcgtcgat
      121 cctgcaccac gagggtgcct cgcccctgtc ggttctcccc gctatcgcgt cgttccatcg
      181 gatccgaccg ctcagcatgc ccgagatcga ggccctgtgg ccgctcgtcg tcattcgcgg
      241 cgccgtgctc gtggcgagcg gatcccagca ggtgcgcaat gacgagtcca atgactacgc
      301 ccgagagggc ctggtccgcg aggagtccat cttccacagc gcatccccgg tgccgtcccc
      361 agtgatgacc cggatcatcg cgcagcacct cgggctcacg tcctcggagt tggcgccgaa
      421 accggcggcc tcccgcctca tcgcgggcga ccattccgta gccctcctcg atctcggggt
      481 gggcagccgc atcctggacg aaggagcttg ggtcaccgac tcgagcgcac gacgcgcggt
      541 cgccgacgcg gtcgacagcc tcttcgcacg cgggaacgac ctggtgatcg cgccgttcgg
      601 cgccatcgat gccagccgca gtcgacccct gtcggtcgat ccggcgtcga cctggcgcac
      661 cgggaccgac gtgtgggtcg ccaccgaggt tcccctcgtg gcgccctggg ctggagagct
      721 gacggtcgcg ccctcgggcc tggtgctgac gggcgaagac gcaaccctcc gcatcaccgc
      781 ggacgagggg atgcccggct cggagccgac gtcggtatcc ctcggagccc cgctcggagt
      841 gctgcgcccg ggcacgtcgt atcgcctcac cgtcgtgacg aacgatctcg agtccgacgt
      901 gcccgccttc gcgccgccag cgtacgccgc cggctggatg gcgtatctcc tcgacccggg
      961 ggcgatcctg ggtatcgggg acgccgccgc gccatccaca gggggcagcg gtgagctcat
     1021 ggagcgtcgg gaagcggtgc ttgccgacgt tcaggagcac tactacgccc agccgcctcg
     1081 catcgagcgg ggatggaagc accacctcat gggcgacgac ggccgggtgt atctcgacat
     1141 ggtgaacaat gtcacggtgc tcgggcacag tcatccgagg gtggcgcgtg cggtcgcaga
     1201 ccaatggcgc accttcaact cgaactcccg gttccactac gggtcgatcg tcgcgctcgc
     1261 cgagaagctg accgccctgc ttccggagag tctcgacacc gtgttcctcg tcaactccgg
     1321 ttcggaagcg gtcgagctcg cgctgcgtct ggcgcgcatc cacaccaagc gtgacgacct
     1381 gctcgccgtg cgggagagct accacgggtg gacgtatctc gccgatgccg tctccacgtc
     1441 ggccgcggac aatccgaacg ccctcgcctc gcggcccgag tgggtgcaca ctctcgacgt
     1501 tccgaacacc ttccgggggc gctatcgcga ggacgaggcc caccgctatg cgcaggatgc
     1561 cacggccgcc gtcagagagc tcgtcgcgtc tgggcggcca ccggcggcct tcatctgcga
     1621 gcccgtttac ggcagcgcgg gcggtgtgac attgcccgag gggtatctgg aggccgtcta
     1681 ctccgaggtg cggcgatcgg gcggcgtgac gatcgccgac gaagtgcagg tcggtctcgg
     1741 tcggctcggc agctggttct ggggcttcga gcagcaggat gtcgtccccg acatcgtcac
     1801 catcgccaag tcgttgggcg atgggcaccc gctcggcgcc gtggtcacca gcaaggccat
     1861 cgccgcgagc tatgccgcgg agggctactt cttctcgtcg accggcggca gcccggtgtc
     1921 atccatcgtc ggtgcaacgg ttctcgacgt catcgccgag gaggacctcg ctggcaatgc
     1981 gcgccgcgtg ggcgggtacc tgaaggggcg cctgcaggag ctcgcgcttc ggcacagcct
     2041 catcggcacc gtgcacgggg agggcctgta cctcggcgtc gagttcgtgc gcgaccgcgt
     2101 ctcactcgag ccagccaccg cggagacctc ggcgatctgc gatcgactgc gtcagctcgg
     2161 cgtgatcgtc cagccgaccg gcaacttcat gaatgtgctc aagatcaagc caccgttgag
     2221 tttcgacgag gatgccgcgg acttcttcgt cgacactctc gatcgcgtcc tggcctgatc
     2281 ctcagcacgg ccaggtcggc ctgtggggcc tctaaccaga accgcggccg tggcccaagc
     2341 cacggagctc ggcattcttc gccagataac gctggcggaa ccaccggttc cgtcataggg
     2401 tgaaatttta cccttccgct tgctgctccg gcagctctga ttgacacgag ggccgtcatg
     2461 acccatccag cgtccaacac catctggctg acgcgcggtg cgcgcgatcg gttggaagcc
     2521 gaattggccg agctgtcgca tggcgccgct ccgagccctg ctgtcgaggg tcgaattcgc
     2581 gagcttcttg ccctgctccg ccgcaccgag gtgggggaca agcccgacga cggacttgtc
     2641 gaagccggca tggtcgtcac ggtgcagttc gagggcgaca gggacgccac tacctttctg
     2701 ctcgcgcatc gaggtgtgac cgtaaccgac tcgaccatag agatcgacat ctactccccc
     2761 gaatcacccc tcggttctgc gattgccaat aagtaccccg gtgattcctt tcactatgaa
     2821 acgcccaccg gatcgcacgt tcggggcacg gtcgtctccg caactccttt caaggaatcg
     2881 gtgcccgctg cacctgcgat ctcgaattag atgaccccca acgccagcat ggcgtccgcc
     2941 accctggtga agccagcgat attcgcccct accacgtagt cgttcggagc tccgtactcg
     3001 tcggcggttt cgaggcagcg atgatgcacc gacgtcatga tctctgacaa acgctcttcc
     3061 gtgtagtcga aggtccatga atcacgggac gcattctgct gcatctcaag agcgcttgtt
     3121 gcgaccccgc ccgcgttaac ggccttgccg ggtccaaatc gcacaccagc atcgcggagc
     3181 gtgtggatgg cacgtggggt cgttggcatg ttcgcgccct cggcgacgac gcggcatccg
     3241 ttcttgatta ggcgaatcgc attttcgtca tctagctcgt tctgcgtggc gcacggcaag
     3301 gcgacatcgc agggcacgtc ccagatcgaa ccgtcagaaa tgaaggatgc ggatgcgcgg
     3361 cgatccgcgt attcgtcaat ccggcctcgt cgttcaatct tgatctcctt gaggagatcg
     3421 aggtcgatcc cggcctcgtc gaccacgtat ccgcttgagt ccgagcacgc caccacacgg
     3481 gcaccgaatt gatgcacctt ctcaatggcg tatattgcga cgttgcccga tcccgatacc
     3541 accaccctct tgccgtcgaa tgactctgca ctggccttca gcatctcctt gacgaagaac
     3601 gcggtcccgt agccggtggc ctccgtgcga acctgcgagc caccccacga cagccctttg
     3661 ccggtgaagg ttccggattc gtatctattt gtgatgcgct tgtactgccc gaacatatag
     3721 ccgatctctc gcccaccgac accgatatcg cctgcgggca cgtcggtgta ctccccgaga
     3781 tgcctgtaga gctccgtcat gaatgactgg cagaaccgca tgacctccgc ttcgctgcgg
     3841 cccttgggat caaaatcgct cccgcccttc gctccaccga taggaagccc gctgagcgag
     3901 ttcttgaaga tctgctcgaa tcccaagaac ttgacgattc cgaggtacac cgatggatgg
     3961 aaccgcaaac cgcccttgta gggtccaagc gcggagttga actcgacacg gaacccacga
     4021 ttcagctgaa cgcgagcccg atcgtcagtc cagggcacgc gaaagataat ctggcgttcg
     4081 ggctcgcaca gccgacgaat cacttcgcta tctgcgtact cgggatgctt ggcgacgacc
     4141 gggccgaggc tctcaagtac ctcccttact gcctggtgaa actcgatctc gccggggttg
     4201 cgggcaatga cctcgtcgaa cacggggacg agatgatcat gcagttgcgg caagggacgt
     4261 tctccaatcg ggggtgctgt gatgccacgc aagctcgttg cgcacactcg acgttatcgc
     4321 tgttgagcca gcggtcagca cgcacccgat ccgttcgttg ccccgcgcgg ctgactcata
     4381 gtgctcgcgc gcgtgagact ccgccatcag catcaaagct gtccacagtg aggtcttacg
     4441 gccgttcact gcaccttggc tgttccatgc ctaccgcgaa gatggcgtgg ctgcgaagtc
     4501 gagaagcgga caacgaactt ggcgagaaag cccctcggac ccttactggg gtgtatgtgg
     4561 tggctccgac gggcgtcgat cccgtgacct cacgattttc agtcgtgcgc tctaccaact
     4621 gagctacaga gccctgcacc tccgcagagg tacaagataa ggcacggcaa tctggcgaga
     4681 gatatgccgt gccttgcgac cctgaccgga cttgaaccgg cgacctccgc cgtgacaggg
     4741 cggcgcgcta accaactgcg ctacagggcc tagctttttc gttctgtgtt tggtgacccc
     4801 aacgggattc gaacccgtgt taacgccgtg aaagggcgcc gtcctaggcc actaaacgat
     4861 ggggcctcga cgtcacagaa cgtctcagct accgagggtc aagcataagg ggcggcgggg
     4921 gaattgccaa acgggtgacg ggatgcgctt gtttgcgcaa cgggggcgaa tgtccgcggc
     4981 atccgggggt ctgaaaggta gtttcaagtc ggagttgaag cttgctgggt tttccagcga
     5041 cctgttgtta ctgttaacac tgttattagt gttatcaacg tgactcgggc acggtgacta
     5101 cacgattcgg ggagcaatga gaatcacgcg cattcatcgg gggatgacga gccgcacggc
     5161 ggcactgccc agcggtatgc ggggcagacg tctcgcggcg attctcgctg tgctcgcgac
     5221 attcgccacc ggcaccatca ttgccagcgc acccgcctac gccaccgact acccgacctg
     5281 gagcgacgtc gctgcggtgc gcaacgacga gtccgcgacg gccaacaaga tcgccgagat
     5341 cgagggcatg ctcgccggcc ttcaggccga agccgagcgc acccagaaag acgccgaagc
     5401 caagggtgtg atctggggca tcgccgacgc gaagttccag gaggccgcct accgcgccga
     5461 caacctgaag aagcaggcgg atgccgcagc cgcaaccgcc ctcgcatccg gtcagcgtgc
     5521 cggccagtgg gccgcccaac tcgcgcgctc cggcggcggg gacatcacca ccgacctctt
     5581 cgcgaacacc ggcgacgccg acaacctgct ctacgggctg ggcatgtcga gcaagatctc
     5641 cgaccaggcg tactccatct acgagcgtgc gctgctcgac cgcaacaccg cgcaggcgct
     5701 gaccgaccag gccgacgtcg ccaagaacga gctcgaggcg ctcaagctgg tcgcggagaa
     5761 ggcgttcaac gaagcgcagg cctccgccga cgccgcgggc aaggcactcg ccgagcagca
     5821 ggacaaccag gcgcgcctgc agcagcagct catcgtgctc aaggagaagc gtgccgcgac
     5881 cgaggccgac tacctggccg gggtgcgcga gcgcatcggc agcggcgcga gcctcgacgc
     5941 cggcgagatc agcctcagcg gctgggcccg tccgacggcc ggccgcatca ccgacggcta
     6001 cggcccacgg gtgtctccgc tgcccggcgc ctcctcgttc catcagggca ctgacatcgc
     6061 ggcctcgtgc ggccagggca tctacgcggc atccagcggc accgtcgtct acgccggctg
     6121 gaacggcacc tacggcaact tcgtgatgat cgaccacggc ggcggcatca ccaccggcta
     6181 cgcgcacatc gtcagcggcg gcatcctcgt ctcctatggc caggaggtcg tcgtcggcca
     6241 gcagatcgcc tctgtcggct cgaccggtct gtcgaccgga tgccacctgc acttcgaggt
     6301 gcgcatcaac ggctactcca ccgactctgt tccgttcatg gctggccagg gaatcactat
     6361 tggctgatac gcggtgtgag agttcggagc ggtagtgtcc aggcacttcc gccccatggg
     6421 ggcgctcgct accgtcgttg tcggcgcggt tctcgttgcc gtcggcatcg tgggccccgt
     6481 ggcggcggag gactacccct cgtgggacga cgtgcaggcc gcccgccaga acgaggccga
     6541 cacacaggcc gccatcgacg agatcgaggg gcttctcgtc ggtctcgaga ccgaggccgc
     6601 cgatctgggc cgcaccgcgc aggtcaaggc tgaggcctac aacgccgcca actccgcgct
     6661 cgaagacgcc accgcggcgg ccgacgcgct cgcgcgtcag tcgaaggatg ccgcggccaa
     6721 ggccaccgaa tccagccagc gtgccggcca gctcatcgcc caactcgcgc gcaccggagg
     6781 cggaagcctc accctggagc tgctcatgag ctcgaacgcc gacgacctgc tcggctcgct
     6841 cggcacgctc acgaaggtca gcgcgcaggc gggcgagatc taccgccagg ccgtcatcga
     6901 caagaatctc gccggctcac tcgccgagca ggccaaggtc gcgcagacca agcgcgccga
     6961 gttggcggcg accgcgaagt ccgcctacac cgaggcccag tccgcatcgg atgccgcgct
     7021 cgcccgcctc gcggagaagc aggccgcctc cgaccagctg tacgcgcagc tcgcctcact
     7081 caagggcacg acctcgtcgg tcgagcaggg ctacctcgag ggactgaccg cggagcagga
     7141 ggggcagaac cagccgcccc cgcccgactc aggtcctgcg cccaacccgc cgccgcccgc
     7201 cccgaacggc tcggccgtgg ccggtgcgct ctcgttcgcc tacgcgcagc tcggtgacat
     7261 gtaccagttc gcgggctccg gccccgacgt gtgggactgc tcgggactca cccgcggcgc
     7321 ctacgcctcg gtcggggtct acatcggcac ccacagcgcg accaaccagt actggacgat
     7381 ggcctacgcc ggccggcttg ttccgctcag cgacatggtt gcgggtgacc tgatcttcta
     7441 cgccgacaac ggtgacatca gcaacagcgc gtaccacgtg accatgtacg tgggcggcgg
     7501 gcagatgatc gaggcgccct acgacggcgt tccggtgcgc gtggtgaacg tgcgctacta
     7561 cgacatcgtg ccgtacgcgg gtcgcccgac gccctaaggc tggttgagta gcgcgaagcg
     7621 cgtatcgaaa ccctcggttg cgctggtttc gatacggcgc tgcgcgccta ctcaaccggt
     7681 cgggctcagt ggccttcggc cttcagcttc tcgaagcccg cgagcacgat ctcctcggcg
     7741 cgcgcggcgc cctcccagcc ctcgaccttg acccacttgc cgggctcgag gtccttgtag
     7801 tgctcgaaga agtgctcgat ctccttgcgg gtgtactcgg gcacgtcgtc gaggtcctgg
     7861 atgtgcgacc agcgcgggtc cttcgcctgc acgcagatca ccttggagtc gatgccggcg
     7921 tcatccgtca tgttgaagac ggcgaccggg cgaaccgaga tggtcacgcc g
//
LOCUS       DAOSOU010000084         7895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_9164_length_7895_cov_6.114796, whole genome shotgun sequence.
ACCESSION   DAOSOU010000084 DAOSOU010000000
VERSION     DAOSOU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9164_length_7895_cov_6.114796"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..216)
                     /locus_tag="PLW15_06045"
     tRNA            complement(146..216)
                     /locus_tag="PLW15_06045"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Gly,seq:tcc)
     gene            347..2218
                     /locus_tag="PLW15_06050"
     CDS             347..2218
                     /locus_tag="PLW15_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33993.1"
                     /translation="MNKPTFSERFRYWFDNWMSRGTLALMALLGIATVVLVLVVGVIV
                     AIFRQTPDDDPERSFGDILWGNLMRTLDPGTMGGDSGWGFRIFMLIITIGGLIIVASL
                     IGIISGAFDSKVEELRKGRSKVLEKDHTLILGWNSKVFPIVSEICIANESRRSAVIVV
                     LADKDKVEMEDELRAMVGKTGKTKIICRTGDPMNLTDLEIANPANARSIILLAGDDSP
                     DPDADVIKTALALTNNPRRKAEKYHIVGELEDAASLEAATLVGRDEAHWVLGDDLIGR
                     ITVQSCRQSGLSVVYSELLDFDGDEIYFTEQPSLYGKTYFDAQLAFVDSTVMGLVKAG
                     SVILNPAADTLYDSGDQLIVIAEDDSAIRLATPGVPDAAAVSTTKALKPKPERTLVLG
                     YNSGLTRMLEELNEYVATGSKVTVLADVDKPDFPKFDNLTIEFRRGDSTNRAILDSLK
                     VESFNHIIVLAYKGLDIQRADGKTLITLLQLRDIEDRAGVDLNIVSEMLDDRNRELAE
                     VTNADDFIVSDKLISLMLSQVSENKKLTQVFDDLFSSVGSEVYLLPVEQYVAPGTAVN
                     FYTVLEAARRRGETALGYRVAANAHKSDKGYGVTVNPVKTETVTFSAGDKIIVLAEG"
     gene            2249..2322
                     /locus_tag="PLW15_06055"
     tRNA            2249..2322
                     /locus_tag="PLW15_06055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2283..2285,aa:Pro,seq:tgg)
     gene            2362..3777
                     /gene="tig"
                     /locus_tag="PLW15_06060"
     CDS             2362..3777
                     /gene="tig"
                     /locus_tag="PLW15_06060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT33994.1"
                     /translation="MKTTVEKLSPTRVKLNISVTPEELKPSIAHAYEHIGSQVNIPGF
                     RKGKIPPQLIDQRVGREEVLNHAVSDGLDKFYRQAVTDEKIRVLGRPEADITTWPEVK
                     DFSGDLEISVEVDVRPEFTLPDYSKLTLTVDAAEVSADDVKDELDNLRSRFGTLVTVD
                     RPAKAGDFAQLDLVAKIGEEEVDSATSISYEVGSGELIEGIDEALDSLSAGESTTFES
                     TLLGGDHEGETALISVTLLAVKERELPEADDDFAQIASQFDTIKELKADIKEQVEKSK
                     VFGQGAQARDQIVDELLKKVEIPVPAKLVEDEVHRHLENENRLEDDVHRAEVVISTEK
                     TFRSQILLDSIVEAEEVKVSQDELTSYLIQGAAQYGMEPGEFIQVLDQNGQIPGMIAE
                     VARVKALAAVLAKAKVVDTKGKAVDVSAFTAAVTGPEGGDFVEASVGSGDSDSHDSHG
                     ATGSDKHGRKAGHEHYGHDHK"
     gene            complement(3836..4000)
                     /locus_tag="PLW15_06065"
     CDS             complement(3836..4000)
                     /locus_tag="PLW15_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT33995.1"
                     /translation="MADIDIRELVAYEPFCADCGESGPIETSAFLAFAWEQAHDCATA
                     AAARDLRAAS"
     gene            complement(4076..4618)
                     /locus_tag="PLW15_06070"
     CDS             complement(4076..4618)
                     /locus_tag="PLW15_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT33996.1"
                     /translation="MDVDVDVDVDVDAGAGVDASDWLRRVSAVWASAGEMADLDVVAA
                     IDALVAERQPDEASAVFEAASARDYAGLESEAEPLYRRAIELGLDDATLPRATIQLAS
                     TLRNLGKHDESVRILERLLAEHPDDEWTGPGAAFLALALASRGDERDAASVALAALAD
                     YLPVYSASVRSYAIELAARE"
     gene            4946..5545
                     /locus_tag="PLW15_06075"
     CDS             4946..5545
                     /locus_tag="PLW15_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT33997.1"
                     /translation="MAEPALVSSIFDQLLKDRIIWLGSEVRDSNANEICAKILLLAAE
                     DPKRDIFLYVNSPGGSITAGMAIYDTMKFVPNDIVTVGIGLAASMGQFLLSSGTKGKR
                     YITPNARVLLHQPSGGFGGTAADIQTQAGVILDMKKRMAELTAEQTGKSVEQILIDND
                     RDNWFTAQEALEYGFVDHLREFASDVVGGGGTVTPENAK"
     gene            5557..6225
                     /locus_tag="PLW15_06080"
     CDS             5557..6225
                     /locus_tag="PLW15_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT33998.1"
                     /translation="MNDFTFGAASGMARQTPESRYILPTFEERTAYGYKRQDPYAKLF
                     EDRIIFLGVQIDDASADDVMAQLLVLESQDPDRDIVMYINSPGGSFTAMTAIYDTMQY
                     IRPHIQTVVLGQAASAAAVITAGGTKGKRLALPNARILIHQPAGGSEGSSQASDIEIQ
                     AREIQRMREWLEETLALHSNRTAEQVHKDIDRDKILSAEEAVEYGLIDQVLSSRKNVP
                     ALVK"
     gene            complement(6323..6964)
                     /locus_tag="PLW15_06085"
     CDS             complement(6323..6964)
                     /locus_tag="PLW15_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HOT33999.1"
                     /translation="MPTIMSRTALALLATTLMVGGSVLGLAASAQAHNYVVASTPGAD
                     EVVTTLPSEFSVTTNAPLLTLGGSTDGFALQVVDSAGQYYGDGCIQVTGATISSGATL
                     GQPGDYTLIWQVVSEDGHPVSGEIAFSWQPADATQESAGSATPPDCGGTIPAPTEPSD
                     SSEPAPGETAVPISAPERPNANLSDVLWIGGAIVAVLGAGLVTFLVIGRRNHS"
     gene            complement(6968..7678)
                     /locus_tag="PLW15_06090"
     CDS             complement(6968..7678)
                     /locus_tag="PLW15_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HOT34000.1"
                     /translation="MKKSLFTSATALIAGAVIALAAPLAASAHVGIDPNQSDPGTYPL
                     ITFKVPTESATATTTKIELTLPQDTPFGFVSYTPVEGWTTELVKETLPEPVVTDDGEV
                     TEAVTKVIWTAEPGSELTAEQYGLFPLVLGPVPDTGSVVLAVDQTYSDGTVVSWSETG
                     ADAEFPAPVLYVNDVPESDHHAGGGADDEHAEIVVDGDTAAAASDDTLARVLGIGGLV
                     LGAVAVVIAVTSRRKKDA"
BASE COUNT         1465 a         2718 c         2512 g         1200 t
ORIGIN      
        1 tcaggggatg acgggaatcc cctactgagc cgccggccga tcgctgcgcg accgcccggt
       61 ctgctcagcg ggggcccatt cgattcactg caccgttatt catgcagaaa gcgcccatcc
      121 ggtggacggg cgctttctgc atgagagggg atgacgggaa tcgaacccgc gtagtcagtt
      181 tggaagactg aagctctacc attgagctac atccccacgg tcggcaagcc gagcgttcgg
      241 cagctagcct aacgcatcat ccgggcgccc gattgaggtg catacagagg gtgtcgctcg
      301 ccgctacgat tcggtagtca gtacgcccga accttagtgg ggacccatga acaagccgac
      361 cttctccgag cgcttccgct attggttcga caactggatg tcgcgaggaa ccctcgcgct
      421 catggccctg ctcggcatcg ccaccgtcgt cctggtgctc gtcgtcggcg tgatcgtggc
      481 catcttccgc cagactcccg acgatgaccc ggagcgcagc ttcggcgaca tcctgtgggg
      541 caacctcatg cgcacgctcg acccgggaac catgggcggc gacagcggct ggggcttccg
      601 catcttcatg ctcatcatca ccatcggcgg cctcatcatc gtcgccagcc tcatcggcat
      661 catctcgggc gcgttcgact cgaaggtcga ggagctgcgc aagggccgct cgaaggtgct
      721 cgagaaggac cacacgctca tcctcggctg gaactccaag gtgttcccga tcgtcagcga
      781 gatctgcatc gccaacgagt cgcgccgcag cgccgtgatc gtcgttctcg ccgacaagga
      841 caaggtcgag atggaggacg agcttcgcgc gatggtcggc aagaccggca agacgaagat
      901 catctgccgc accggcgacc cgatgaacct caccgacctc gagatcgcca accccgcgaa
      961 cgcgcgcagc atcatcctgc tggccggcga cgacagcccc gaccccgacg ccgacgtgat
     1021 caagaccgcg ctcgcgctca ccaacaaccc ccgccgcaag gcggagaagt accacatcgt
     1081 cggcgagctc gaagacgccg cgagcctgga ggcggcaacg ctggtgggcc gcgacgaggc
     1141 gcactgggtg ctgggcgacg acctgatcgg tcgcatcacc gtgcagagct gccgtcagag
     1201 cggcctgagc gtcgtctact ccgagctgct cgacttcgac ggcgacgaga tctacttcac
     1261 cgagcagccg agcctctacg gcaagacgta cttcgacgcg cagctggcct tcgttgactc
     1321 gactgtgatg ggcctcgtga aggcgggttc ggtcatcctg aacccggcag ccgacacgct
     1381 ctacgacagc ggcgaccagc tcatcgtcat cgccgaggac gacagcgcca tccgtctcgc
     1441 tacccccggt gttccggatg ccgcggccgt cagcaccacg aaggccctca agccgaagcc
     1501 cgaacgcacc ctcgtgctcg ggtacaactc ggggctcacg cgcatgctcg aggaactgaa
     1561 cgagtacgtg gctaccggat ccaaggtcac ggtgctcgcc gatgtggaca agcccgactt
     1621 cccgaagttc gacaacctga cgatcgagtt ccgtcgcggc gattcgacga accgcgccat
     1681 cctcgacagc ctgaaggtgg agtcgttcaa ccacatcatc gtgctcgctt acaagggtct
     1741 cgacatccag cgcgccgacg gcaagacgct catcacgctt cttcagctgc gcgacatcga
     1801 ggaccgcgcg ggcgtcgacc tcaacatcgt cagcgagatg ctggatgacc gcaaccggga
     1861 acttgccgag gtcaccaacg ccgacgactt catcgtcagc gacaagctga tcagcctgat
     1921 gctctcgcag gtgtcggaga acaagaagct cacccaggtc ttcgacgacc tgttctcgag
     1981 cgtcgggtct gaggtgtacc tcctgccggt cgagcagtac gtcgcgccgg gcaccgccgt
     2041 gaacttctac acggtgctcg aggccgctcg ccgccgcggc gagaccgccc tcggttaccg
     2101 cgtcgccgcc aacgcgcaca agagcgacaa gggctacggc gtcaccgtga acccggtcaa
     2161 gacggagacg gtcacgttct cggcaggcga caagatcatc gttctcgcag agggctagac
     2221 ttactcggtt gtcaattcgc cttagaagcg gggcgtagct cagcttggta gagcgctcgg
     2281 tttgggaccg agaggccgca ggttcgaatc ctgtcgcccc gacacgtacg accacgacac
     2341 acgatccagg agaaccacag cgtgaagacc accgtcgaga agctgagccc cacccgcgtc
     2401 aagctgaaca tctcggtcac ccccgaagag ttgaagccga gcatcgccca cgcctacgag
     2461 cacatcggct cgcaggtcaa catccccggc ttccgtaagg gcaagatccc gccgcagctc
     2521 atcgaccagc gcgtcggccg cgaagaggtt ctgaaccacg cggtcagcga tggcctcgac
     2581 aagttctacc gccaggcggt caccgacgag aagattcgcg tgctcggtcg ccccgaggcc
     2641 gacatcacca cctggcccga ggtcaaggac ttcagcggtg acctcgagat cagtgtcgag
     2701 gtggatgtcc gccccgagtt caccctgccg gactacagca agctgaccct cacggtcgat
     2761 gccgccgagg tctccgctga cgacgtcaag gacgagctcg acaacctgcg cagccgcttc
     2821 ggcactctcg tgacggtcga ccgcccggcc aaggccggcg acttcgccca gctcgacctg
     2881 gtcgcgaaga tcggcgaaga agaggtcgac tcggccacga gcatctccta cgaggtcggt
     2941 tcgggtgagc tcatcgaggg catcgatgag gccctcgaca gcctgagcgc tggcgagtcc
     3001 acgaccttcg agtcgactct gctcggtggc gaccacgagg gcgagaccgc gctcatcagc
     3061 gtgaccctgc tcgcggtcaa ggagcgcgag cttcccgagg ccgacgacga cttcgcccag
     3121 atcgccagcc agttcgacac catcaaggaa ctcaaggccg acatcaagga gcaggtcgag
     3181 aagtcgaagg tcttcggtca gggcgcccag gcgcgcgacc agatcgtcga cgagctgctc
     3241 aagaaggtcg agatccccgt tcccgccaag cttgtggaag acgaggtgca ccgtcacctg
     3301 gagaacgaga accgcctcga ggatgacgtg caccgcgccg aggtcgtcat ctccacggag
     3361 aagacgttcc gctcgcagat ccttctcgac tccatcgtgg aggccgagga ggtcaaggtc
     3421 agccaggacg agctgacgag ctacctcatc cagggtgccg cccagtacgg catggagccg
     3481 ggcgagttca tccaggtgct tgaccagaac ggccagatcc ccggcatgat cgccgaggtc
     3541 gcccgcgtca aggcgctcgc cgccgtgctc gccaaggcga aggtcgtcga caccaagggc
     3601 aaggcagtgg atgtctcggc cttcaccgca gccgtcaccg gccccgaggg cggcgacttc
     3661 gtcgaggcct cagtgggctc gggagacagc gactcccacg acagccacgg tgcgaccggc
     3721 agcgacaagc acggccgcaa ggccggccac gagcactacg gtcacgacca caagtagcgc
     3781 gtgacgacag aacggccccg gggtgtgatc cccggggccg ttgtcgtttc aggggtcagg
     3841 atgccgcgcg aaggtcgcgt gcggcggccg cggttgcgca gtcgtgggcc tgctcccacg
     3901 cgaacgcgag gaacgcgctc gtctcgatcg gaccgctctc gccgcagtca gcgcagaacg
     3961 gctcgtacgc gaccagttca cggatgtcga tgtctgccat gcctctatgc tcaccccgac
     4021 ctccgacatc gcgggggagg cacgcgggca agccgctcgc cggagtgaaa ccgagctact
     4081 cgcgcgccgc caactcgatc gcgtagctgc gcaccgacgc cgagtacacc ggcaggtagt
     4141 cggcgagggc cgcgagggcg acggatgccg catcccgctc gtctccccgg ctcgccagcg
     4201 cgagcgcaag gaacgcggcg cccggacccg tccactcgtc gtccggatgc tcggcgagca
     4261 gccgctccag gatcctcacg ctctcgtcgt gcttgccgag gttgcgcagg gtgctggcga
     4321 gctggatcgt cgcgcggggg agggtcgcgt catccagacc cagctcgatc gccctgcggt
     4381 agagcggctc agcctcggac tcgaggcccg catagtcgcg ggccgaggcc gcctcgaaga
     4441 ctgcggatgc ctcatccggc tgccgctcgg cgaccagggc atcgatcgcc gccaccacat
     4501 cgaggtcggc catctcgccg gccgatgccc acaccgcgct cacgcgccgc agccagtcgc
     4561 tcgcatccac acccgcaccc gcatccacat ccacatccac atccacatcc acatccacgc
     4621 cgacacccta gccgttcccc acctcgcgac ccgcacgcgg atcaggacaa cggcgcgaca
     4681 cgccgctagc tgcccgcagg ggagcggcgc gccgtgcatc gttcctgatc gacgaacagg
     4741 gggatgccgc gggccggagg tccatcgtgt ccacggcggc tgtgcgtggt ggttcgcgtc
     4801 gacagagcgc cgctctgccc acggcggctg tgcttggtgg ttcgccgcgg acagagcgcc
     4861 gctctgccca cggcgaacac gggcgatcgg ccgcgcgcgc tcggctagat tcgacacaag
     4921 caataactga aacggagcgc accccatggc cgaaccggca ctggtctcga gcattttcga
     4981 ccaactcctc aaggaccgca tcatctggct cgggtcagag gtgcgtgact ccaacgccaa
     5041 cgagatctgc gcgaagatcc tcctcctcgc cgccgaggac ccgaagcgcg acatcttcct
     5101 ctacgtgaac tcgcccggtg gctcgatcac cgcgggcatg gccatctacg acacgatgaa
     5161 gttcgttccg aacgacatcg tcaccgtggg catcggcctc gcggcatcca tgggccagtt
     5221 cctgctctcg tcgggcacga agggcaagcg ctacatcacc ccgaacgccc gcgtgctgct
     5281 gcaccagccg tccggtggtt tcggcggtac ggccgccgac atccagaccc aggcgggcgt
     5341 catcctcgat atgaagaagc ggatggccga gctcaccgcc gagcagaccg gcaagtcggt
     5401 cgagcagatc ctgatcgaca acgaccgcga caactggttc accgcccagg aggcgctcga
     5461 gtacggcttc gtggatcacc tccgcgagtt cgcatccgat gtcgtcggcg gcggcggaac
     5521 cgtcaccccc gagaacgcca agtaaggact gacctgatga acgacttcac tttcggcgcc
     5581 gcatcgggga tggctcgcca gacccccgag tcccgttaca tcctgccgac cttcgaggag
     5641 cgcacggcct acggctacaa gcgccaggac ccgtacgcga agctcttcga ggaccgcatc
     5701 atcttcctcg gtgtgcagat cgacgacgcg tcggccgacg acgtgatggc ccagctgctc
     5761 gtgctcgaga gccaggaccc cgaccgcgac atcgtcatgt acatcaactc gcccggtggc
     5821 tcgttcaccg cgatgacggc gatctacgac acgatgcagt acatccgtcc gcacatccag
     5881 acggtcgtgc tcggtcaggc cgcgtcggcc gccgcggtca tcaccgcggg aggcaccaag
     5941 ggcaagcgtc tcgcactgcc gaacgcgcgc atcctcatcc accagcccgc cggcggatcc
     6001 gagggctcga gtcaggcatc cgacatcgag atccaggccc gcgagatcca gcggatgcgc
     6061 gaatggcttg aggagacgct cgccctgcac tcgaaccgca cggccgagca ggtgcacaag
     6121 gacatcgacc gcgacaagat cctgagcgcc gaggaggccg tcgagtacgg actcatcgac
     6181 caggtgctct cgagccgcaa gaacgtgccg gcgctcgtca agtagcccga gcaccgcaga
     6241 accgagcacc gcagaacacc agcactgcag aactaccgaa gggccgccct cacgagaagg
     6301 cggcccttcg gcgtttccgc ttctagctgt ggttacggcg cccgatgacg aggaacgtca
     6361 ccaggccagc accgagcacg gcgacgatcg cgccgccgat ccagagcacg tcgctcaggt
     6421 tcgcgttcgg gcgctccgga gcggagatgg gcacggctgt ctccccgggc gcgggctccg
     6481 aggagtccga cggctcggtc ggcgccggga tcgtgccgcc gcagtcggga ggggtcgccg
     6541 atcccgctga ctcctgcgtg gcatccgctg gttgccagct gaaggcgatc tcgcccgaca
     6601 ccgggtggcc gtcttcggag acgacctgcc agatcagcgt gtagtcgccc ggttggccga
     6661 gagtcgcgcc cgacgagatg gtcgcgcccg tgacctggat gcagccgtcc ccgtagtact
     6721 ggcccgccga atcgacgacc tgcagcgcga acccgtcagt gcttcccccg agcgtcagca
     6781 gcggagcatt ggtcgtgacg gagaactcgc tcggcagcgt ggtgaccacc tcatctgcgc
     6841 cgggggtcga cgcgacgacg tagttgtggg cctgtgccga ggcggcgagc cccagcacag
     6901 acccgccgac catcagcgtc gtggcgagca gggccagagc cgtgcgactc atgatggtcg
     6961 gcatcgacta cgcgtccttc ttgcgacggg aggtcactgc gatcaccacg gcgaccgcac
     7021 ccaacacgag gccgccgatt cccagcacac gagcgagcgt gtcatcggat gccgcagccg
     7081 cagtatcccc gtcgacgacg atctcggcgt gctcgtcgtc cgccccaccg ccagcgtggt
     7141 ggtcggactc gggcacgtcg ttcacgtaga gcaccggcgc ggggaactca gcgtcggccc
     7201 cggtctcgct ccagctcacg acggtgccgt cgctgtacgt ctggtcgacg gcgagcacga
     7261 ccgatcctgt gtcgggcacg ggtccgagca cgagcgggaa cagtccgtac tgctccgcgg
     7321 tcagctccga tccgggctcg gccgtccaga tgaccttggt caccgcttcg gtgacctcac
     7381 cgtcgtcggt gaccacgggc tcggggaggg tctccttgac gagctcggtg gtccagccct
     7441 cgaccggggt gtagctgacg aagccgaacg gggtgtcctg cggaagggtc agctcgatct
     7501 tggtcgtggt tgcggtcgcc gactccgtgg gtaccttgaa ggtgatcagg ggataggtgc
     7561 cggggtccga ctggttggga tcgataccga cgtgggcgct cgccgccagc ggtgcggcta
     7621 gggcgatcac cgcaccggcg atgagcgcgg tagcggaggt gaacagggat ttcttcattc
     7681 gtgtagtcct tcaatggcgg tcaccgaacg gcgaccgaga gtggtggggc aagccccggg
     7741 gaatcgtgtg ggcgctatgc gcccgcaact cccagcggtg gaccgcgccg actcagcgaa
     7801 gaactcacga gaggaaccag gtatggcggg cgagcgtcga tcgccgtcag aaatacacgg
     7861 cttggcgtca caaccacggg cacgagcacc cgggt
//
LOCUS       DAOSOU010000085         7717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_9444_length_7717_cov_6.976116, whole genome shotgun sequence.
ACCESSION   DAOSOU010000085 DAOSOU010000000
VERSION     DAOSOU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_7717_cov_6.976116"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLW15_06095"
     CDS             complement(<1..937)
                     /locus_tag="PLW15_06095"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT34001.1"
                     /translation="MAERIDTLLILGAGGDLTSRLLLPGVASYLGSERGEKLTVIGVD
                     RGEVTPAQWRERVTTAFATENSRLAASVAKSSIYLQADATNPADLARVLAQAPGRIAV
                     YFALPPAVTLLAVKALDSLELPKGILFALEKPFGTDLATAKSLNRRLQKMLPEEQLFR
                     VDHFLGKSTVLNLLGLRFANRIFEPLFSAPNIERVEITFDEELALEGRAGYYDKAGAL
                     VDMIQSHLLLVLALVAMEPPASVDSEDLRGGMAQVLRATRPWRKNGTSSRRARYGAGT
                     IDGRRLPAYVDEKGVDPGHGTETLAELTLAIDNWRW"
     gene            1082..1732
                     /locus_tag="PLW15_06100"
     CDS             1082..1732
                     /locus_tag="PLW15_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34002.1"
                     /translation="MVMRGEREYAIKAWTNPAISITSTDHSIVMTPTGTASGDGLVFI
                     PGAKVDPYAYMFKLSGIVETAGVTVVITKPTLNLAFFDTRPLSTFTADAPGVDSWFVG
                     GHSLGGVRSCMLADDPDVAGLVLFGSYCANDLSATELEVLSIGGSEDGLSTPQKIADA
                     SSLLPATATFVQVEGLNHAGFGNYGVQSGDGVATITSAEARDEITAALVGFFDAPR"
     gene            complement(1729..2430)
                     /locus_tag="PLW15_06105"
     CDS             complement(1729..2430)
                     /locus_tag="PLW15_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34003.1"
                     /translation="MKAHLKGSHYVAAIIAGIVGNIFFSAGWSLLGVTLFFGLFAAIF
                     GGSIESIIGQFTDPAAISGFFSSAGGIAAGLTVGFSIAALVLVGLGVLFSGLILKGGK
                     VRKPWATTWLSVLISALLSVPLFFVWLAIANNTNGAPIALVVVLGTAVVGILIWLWMT
                     WAHRGYASEFAGASASATATATAKIAPADAPAEVEAAPVAEAPVAAAPVAKAPAAKTT
                     AAKPPAAKKTPPASS"
     gene            2502..2942
                     /locus_tag="PLW15_06110"
     CDS             2502..2942
                     /locus_tag="PLW15_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34004.1"
                     /translation="MNSWRDDVSAQAQTQLDSLLDSALSLAQRNLAKASDFDPFAVFL
                     SEDGRYILVAYDKESKGKPPSVETILVNLLDQLRQLERDAVCTAIVLNSRLEKERTDA
                     IEVRLEHKAGGSLVVLLPYKRALFGGTTEYGQLKGFSGKRLVWA"
     gene            3009..3497
                     /locus_tag="PLW15_06115"
     CDS             3009..3497
                     /locus_tag="PLW15_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HOT34005.1"
                     /translation="MVRVQPEPGQESVWDYPRPPRVESTRERVVVRLGGAVIVDTVAA
                     LRVLETSHPPAYYLPRAAFPDGALVPAAGTSFCEYKGLARYLTIRTPDATAERSAWFY
                     PTPSPGYEQLADYVSLYPAAMDSCTVDGETVTPQAGGFYGGWITSRVVGPFKGEPGTL
                     GW"
     gene            complement(3516..3944)
                     /locus_tag="PLW15_06120"
     CDS             complement(3516..3944)
                     /locus_tag="PLW15_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT34006.1"
                     /translation="MSRTDDLAAAITAFFRALESSRSRDALEVQLTPTEYRALARVVE
                     VGNKTPKRLAISMGMSTGAITAISDSLVARGLLERVANEADRRSVVLRPTPMGRELVG
                     RLFGNYSRVIGAAVEGLSDAEVQQLTVVLHNLTEGLNARS"
     gene            4110..4946
                     /locus_tag="PLW15_06125"
     CDS             4110..4946
                     /locus_tag="PLW15_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT34007.1"
                     /translation="MPTLAEIADELYGLLPAEFTAARNVWVRALKDETDTELAERVSV
                     LRKASPAAWGVNLLARHRSDELAQLADLGALMRQAQSDLDRDQLAELTSQRRRLVSAL
                     AREAAELAADAHHVLTSSALDEVAATLQAALGDPAAAAAVRSGRLVRPLVGDGVEPVD
                     LDDAVAAPEASMVSAVPRAARRAPVRDLAVVRAAKEAEKRADAADTEVTGIDRRIESV
                     ESARTRLQSRLEQLEQQVTEVEDELRESDRDLRALGRDRDRAARRAEDLRAEAVRLRA
                     ED"
     gene            complement(4952..6028)
                     /locus_tag="PLW15_06130"
     CDS             complement(4952..6028)
                     /locus_tag="PLW15_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT34008.1"
                     /translation="MGARTRIASVTRWARRLFPVRVWLHFLDRNGFLLAAGMSYQALF
                     AVFAAAYVGLSIAGFVVVGNEAVLDAAITLINTWVPGLLGADGIIDPEAFRATTTVLS
                     WTGLVALGSLIWTAIGWVTYSRMAVRTVFGLEKDKRNYVVMKARDLLVAAAFGVALLV
                     AAALSVASTELLDWIFSLLGWSTSSEVFRISVRLSGLVLVFAIDTLALAAMFRFLSRA
                     AIQWRRLWGGSLLGGAGLVALQLLGSLLAGGITRNPLLATFAVFVGLLLFFRLTSIVT
                     LVAAAWIAVGAHDRDESLRLVSPEQLDRERADAEHRALLLAAQVRVREARAALDAANW
                     FERPSARRHLRGAEEELEQLRDRT"
     gene            6072..6977
                     /locus_tag="PLW15_06135"
     CDS             6072..6977
                     /locus_tag="PLW15_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT34009.1"
                     /translation="MSATLPVVVPDLSGRLAVITGANSGLGFGLAQRISAAGADVILA
                     VRNRAKGDDAAARLGTGRVELVDLASLQSIRAFADARIAEGRPVDILINNAGVMAPPK
                     RGVTVDGFELQFGGNYLGAFALTGRLLPLLRKAEHPRVTMLSSIYARRGRLDFDDLQS
                     EKKYVPYTAYGQSKLADLMFTRELQKRSDDRGWGILSTAGHPGGTATNLQTTGPREGR
                     EYGHLGRRLADAFLQPVAQGILPALYAATSQDATPGAYYGPDGFLELRGGVKLAHVPA
                     RALRDDDSARLWDVSQSLTGVTFGA"
     gene            complement(6982..7632)
                     /locus_tag="PLW15_06140"
     CDS             complement(6982..7632)
                     /locus_tag="PLW15_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT34010.1"
                     /translation="MAELDGRVVLVVGASGGLGSRLVAELDAAGATVLSASRSTTASL
                     DLSDPQSIRDYVGGLTALDGVVIAAGVVAFGPADQVSDGVLAELFVANAVGPITLIRE
                     LTPLLAESAAAGRTPFIVTLSGIVAESPTAGLAAYSASKSALAAFVSAASREFRRTGI
                     RVLDARPGHTETELSRHPIEGTAPAFPPGLDPARVASRIVRAIVADEKDLPSSAFA"
BASE COUNT         1257 a         2679 c         2643 g         1138 t
ORIGIN      
        1 cccagcgcca gttgtcgatg gccagcgtca gttcggcgag agtctcggtg ccgtggccgg
       61 ggtcgacgcc cttctcgtcc acataggcgg gcagtcgccg gccgtcgatc gtgccggcac
      121 cgtatcgcgc acgccggctg gaggtgccgt tcttgcgcca cggccgggtc gccctcagca
      181 cctgagccat gccgccgcgc aggtcctccg aatcgacact ggccggcggc tccatcgcga
      241 ccagcgccag caccagcagc aggtggctct ggatcatgtc cacgagggcg cccgccttgt
      301 cgtagtagcc cgcgcggccc tcgagcgcca actcctcgtc gaacgtgatc tccacccgct
      361 cgatgttcgg cgcggagaac aacggctcaa agatgcggtt cgcgaagcgc aaccccagga
      421 ggttgagcac ggtcgacttg ccgaggaaat ggtcgacacg gaacagctgc tcctcgggca
      481 gcatcttctg cagcctccgg ttgagcgact tcgccgtggc caggtcggtt ccgaacggct
      541 tctccagcgc gaacaggatg cccttcggca gctcgaggga gtccagcgcc ttgaccgcga
      601 gcagggtgac cgcgggcggc agcgcgaagt acacggcgat gcggcccgga gcctgcgcca
      661 gcaccctggc cagatcggcg gggttcgtgg catccgcctg cagatagatc gatgacttgg
      721 cgacggatgc cgccagccga gagttctccg tggcgaacgc ggtggtcacg cgctcgcgcc
      781 actgggccgg ggtcacctcg ccgcggtcca ccccgatcac cgtcagtttc tcgccgcgct
      841 cgctgcccaa gtagctcgcg acgccgggca ggagcagccg ggaggtgagg tcgccgccgg
      901 cgccgaggat gagtagggtg tcgattcgtt cggccacggg ccgaccctac tccagaatgg
      961 tgccatgact gattcgacga tgccgggcgc gcgccggttc ctgcgcggcc tcggtcgcac
     1021 cgtgctggcg atcatcgtgg tgctcgtgct gctggtggtc ggcttcctgt tctacgcgaa
     1081 catggtgatg cgcggcgaac gcgagtacgc catcaaggcg tggaccaacc ccgctatctc
     1141 gatcacctcg accgaccact cgatcgtgat gaccccgacc ggcactgcct cgggcgacgg
     1201 tctcgtgttc atcccgggcg cgaaggtcga cccctacgcc tatatgttca agctcagtgg
     1261 gatcgtcgag acggccgggg tgacagtggt catcaccaag cccacgctca acctcgcgtt
     1321 cttcgacacc cgtccgctct cgacgttcac ggccgacgca cccggcgtcg acagctggtt
     1381 cgtcggcggg cactccctgg gtggggtgcg cagctgcatg ctcgccgacg acccggatgt
     1441 cgcgggcctc gtgctgttcg gcagctactg cgccaacgat ctgtccgcca ccgagctcga
     1501 ggtgttgtcg atcggcggca gcgaggacgg cctgagcact ccgcagaaga ttgcggatgc
     1561 gtcatccctg ttgccggcga cggccacctt cgtgcaggtg gagggcctca atcacgcggg
     1621 attcggcaac tacggcgtgc agtcgggtga cggagtcgcg acgatcacga gcgccgaggc
     1681 gcgcgacgag atcaccgcgg cgctcgtcgg attcttcgac gcgccccgct aggaactcgc
     1741 tggcggagtc ttcttcgcgg cgggcggctt cgctgccgtg gtcttcgccg cgggcgcctt
     1801 cgcgaccgga gcggccgcga cgggagcctc ggcgaccggc gccgcctcga cctcggctgg
     1861 cgcatccgcc ggcgcgatct tcgctgtagc cgtggccgta gccgacgcag aagcgccagc
     1921 gaactcgctc gcgtagccgc ggtgcgccca cgtcatccag agccagatca ggatgcccac
     1981 gaccgccgtg ccgagcacga ccaccagggc gatgggagcc ccattggtgt tgttggcgat
     2041 cgccagccag acgaagaaca gcggaaccga cagcagcgcc gagatgagca cggacagcca
     2101 ggtcgtcgcc cacggcttgc gcaccttgcc gcccttgagg atcagaccgc tgaagagcac
     2161 gccgaggccc accagcacca gagccgcgat cgagaagccg accgtcagtc cggcagcgat
     2221 gccgccggca ctggagaaga agccggagat cgcggcggga tcggtgaact gcccgatgat
     2281 cgactcgatc gacccgccga agatcgcggc gaacaggccg aagaacagag tgacgccgag
     2341 caggctccac ccggccgaga agaagatgtt gccgacgatg cccgcgatga tcgccgcgac
     2401 gtagtgactg cccttcaggt gtgctttcat gcaaacaccc tatggcctac cccccgtagt
     2461 gtcgccagcg gtgaacggcc ttgactcggt agcgtttgag catgaactcg tggagggacg
     2521 acgtcagcgc ccaggcgcag acccagctcg actccctcct cgactccgcg ctgtccttgg
     2581 cgcagcgcaa tctcgccaaa gcgagcgact tcgacccgtt cgcggtgttc ctcagcgaag
     2641 acggccggta catcctcgtc gcctatgaca aggagtccaa gggcaagccg ccgtcggtcg
     2701 agacgatcct ggtcaacctc ctcgaccagc tgcggcagct cgagcgcgat gccgtctgca
     2761 ccgccattgt gctgaacagc cgcctggaga aggagcgcac cgacgcgatc gaggtgcgcc
     2821 tcgagcacaa ggccggcgga tccctcgtcg tgctgctgcc ctacaagcgc gcactgttcg
     2881 gcggcacgac ggaatacggg cagctcaagg gcttcagcgg caagcggctc gtctgggcgt
     2941 agccttcgtc gaacccggcc gatccctccc gcacagccct cgggcgtagg ctcgacaggg
     3001 tggcgaagat cgtgcgcgtg caaccggaac ccgggcagga gtccgtgtgg gactacccgc
     3061 ggccgccgcg ggtggagtcc actcgcgagc gcgtcgtggt tcggctcggc ggcgcggtga
     3121 tcgtcgacac cgtggctgcg ctgcgggtgc tggagacgag tcatccgccc gcctactatc
     3181 tgccgcgcgc cgccttcccc gacggggctc tcgtgcccgc cgcgggcacc agcttctgcg
     3241 agtacaaggg tcttgccagg tacctgacga tccgcacacc ggatgccacg gccgagcgat
     3301 cggcttggtt ctacccgacc ccgtcccccg ggtacgagca gctcgccgac tacgtctccc
     3361 tgtacccggc ggccatggac tcgtgcacgg tggacgggga gaccgtcacc ccgcaggccg
     3421 gagggttcta cggcgggtgg atcacgtcgc gcgtcgtcgg ccccttcaag ggagaaccgg
     3481 gcacactcgg ctggtgaggc cgcgcggcga tcaggtcagc tcctggcgtt gaggccctcg
     3541 gtgaggttgt ggagcacgac cgtgagctgc tgcacctcag cgtcggacag gccctcgacc
     3601 gcagcgccga tcacacggct gtagttgccg aacagtcggc ccaccagctc acgacccatc
     3661 ggggtgggcc gcagcaccac gctgcgacgg tcggcctcgt tggcgacgcg ctcgagcaat
     3721 ccgcgagcca ccaggctgtc cgagatggcg gtgatggcgc ccgtcgacat ccccatcgag
     3781 atggcgagac gcttcggggt cttgttgccg acctccacca ccctggccag tgcccggtat
     3841 tccgtcggag tgagctgcac ctcgagcgca tcgcggctgc gcgaactctc cagcgcgcga
     3901 aagaacgcgg tgatcgctgc ggcgaggtca tcggtcctgc tcacggcccc ggcttcagtg
     3961 gtggtggtcg gctgcgtcac acaagccatt ctgccgtaga gccccgcccg cgcggatgcg
     4021 agacggaatc gagttcctcg tccgccggaa atgctgcccg cgcagcgagg gtactggcgg
     4081 cagcagactg atcgtgttcg gatggtcaca tgccgacact cgccgagatc gccgatgaac
     4141 tgtacgggct gctgcccgcc gagttcaccg ctgcgcgcaa cgtgtgggtg cgcgcactca
     4201 aagacgagac cgacaccgag ctcgccgagc gcgtgagcgt gctgcgcaag gcctcgcccg
     4261 cggcgtgggg tgtgaatctg ctggctcgac accgttctga cgaactcgcc cagctcgcag
     4321 atctcggcgc actgatgcgc caggcccagt ctgacctcga ccgcgaccag ctggccgaac
     4381 tgacctcgca gcgtcgccgc ctcgtgtcgg cactcgcccg cgaggcggcg gagcttgcgg
     4441 cggacgcgca ccacgtgctc accagctcgg cactcgacga ggtcgccgcg acgctgcagg
     4501 cagcgctcgg ggatccggcg gccgctgcgg cggtgcgcag tggccgactc gtgcggcccc
     4561 tggtgggcga cggtgttgag cccgttgatc tggatgacgc tgtggccgcg cccgaggcat
     4621 ccatggtgtc ggccgttccg agggccgccc ggcgcgcgcc cgtgcgagac ctcgccgtcg
     4681 tgcgtgcggc caaggaggcg gagaagcgcg cggatgccgc cgacaccgag gtgacgggca
     4741 tcgaccgccg catcgagtcg gtcgagtcgg cgcgcacacg actgcagtcc cggctggagc
     4801 agctggagca gcaggtcacc gaagtggagg acgagctgcg cgagtccgat cgcgacctgc
     4861 gggcgctcgg ccgggaccgc gatcgcgcgg cacggcgtgc cgaggacctg cgggccgagg
     4921 ccgtgcgcct gcgcgcggag gactgagcga cctaggttcg gtcgcgcaac tgctcgagct
     4981 cctcctcggc acctctcagg tgtcgcctcg ccgacgggcg ctcgaaccag ttcgccgcgt
     5041 cgagggcggc gcgagcctcc cgcacccgaa cctgcgcggc gagcagcagc gcgcggtgtt
     5101 cggcgtcggc tcgttcgcgg tcgagttgtt cggggcttac caggcgcagc gactcgtcgc
     5161 gatcgtgggc gccgacagcg atccacgctg ccgcgacgag cgtcacgatg ctggtcagac
     5221 ggaagaacag cagcagaccg acgaatacgg cgaaagtcgc cagaagcggg ttgcgggtga
     5281 tgccacccgc gaggagtgag ccaagcagtt ggagtgcgac gaggccagca ccaccgagga
     5341 gggaaccgcc ccagagtcgc cgccattgga tcgctgcgcg ggagaggaac cggaacatcg
     5401 cggcgagcgc cagggtgtcg atcgcgaaga cgagcacgag gccggacagc cgtacgctga
     5461 tgcggaacac ctcggagctg gtgctccacc cgagaagcga gaagatccag tccagcagct
     5521 cggtgctcgc cacggagagc gccgccgcga cgagcagggc gacgccgaag gcggcggcga
     5581 ccagcagatc ccgagccttc atgacgacgt agttgcgttt gtccttctcg agaccgaata
     5641 ccgtccgcac cgccatgcgc gaataggtca cccacccgat ggcggtccag atcaggctgc
     5701 cgagggcgac gaggccggtc cagctgagca ctgtcgtcgt cgcgcggaag gcctcgggat
     5761 cgatgattcc gtcggccccg agcaggccgg gcacccacgt gttgatgaga gtgatcgcgg
     5821 catccagcac cgcttcgttg ccgacgacca cgaagccggc gatcgacagt cccacgtagg
     5881 ccgcggcgaa caccgcgaag agggcctgat agctcatgcc cgcggcgagc aggaacccgt
     5941 tgcggtcgag gaagtgcagc cacacccgca ccgggaacag cctgcgtgcc caccgggtga
     6001 cgctcgcgat cctcgtcctc gctcccacgg cacgagacta tccgacgccg cccgcgtggt
     6061 tggatggtga catgtcagcc actctgcccg tcgtcgttcc cgatctcagc ggacgccttg
     6121 ccgtcatcac cggcgccaac agcggcctgg gcttcgggct cgcgcagcgc atcagcgcgg
     6181 cgggtgccga cgtgatcctc gccgtgcgca atcgcgccaa gggcgacgat gcggcggcac
     6241 gactcggcac cgggcgggtc gagctggtcg acctcgcgtc gcttcagagc atccgcgcct
     6301 tcgccgacgc tcgaatcgcc gaggggcggc cggtagacat cctgatcaac aacgccggag
     6361 tcatggcacc cccgaagcgt ggcgtgaccg tcgacggctt cgagctgcag ttcggcggca
     6421 actacctcgg cgcgttcgcg ctgaccgggc ggctgctgcc ccttctgcgt aaggccgagc
     6481 acccccgcgt gacgatgctg tcgagcatct acgccaggcg cggccgactc gacttcgacg
     6541 acctgcagtc ggagaagaag tacgttccgt acaccgccta cggccagtcc aaactcgccg
     6601 acctgatgtt cacgcgcgag ctgcagaagc gcagcgatga ccgtggctgg ggcatcctca
     6661 gcaccgctgg ccacccgggt ggaaccgcca ccaacctgca gaccacgggc ccgcgcgagg
     6721 gtcgcgagta cggtcacctc ggccgtcgtc tggccgatgc cttcctccag cccgtggcgc
     6781 agggcatact gcccgctctc tacgcggcga cgagccagga tgccaccccc ggcgcctact
     6841 acggaccgga cggcttcctc gagctgcgcg gcggcgtgaa gctcgcgcac gtacccgctc
     6901 gtgccctgcg cgacgatgat tcggcgcggc tctgggatgt ctcgcagtcg ctgacgggcg
     6961 tgacgttcgg cgcgtagcgc cttacgcgaa ggcgctgctc ggcaggtcct tctcgtccgc
     7021 gacgatcgcc cgcacgatgc ggctcgcgac cctcgcgggg tccaggcccg gcgggaacgc
     7081 gggcgcggtt ccctcaatcg gatgccgcga cagttcggtc tcggtgtggc cgggccgcgc
     7141 atccagcacg cggatccccg tgcgtctgaa ctcccgcgac gccgcggaca cgaacgcggc
     7201 gagcgccgac ttggacgccg agtaggcagc cagtcccgcg gtcggggact ccgcgacgat
     7261 gccgctgagc gtgacgatga acggcgtgcg gccggcggca gccgactccg cgagcagggg
     7321 cgtcagctca cgaatgagag tgatcggacc taccgcgttg gccacgaaca actcggcgag
     7381 cacgccgtcg gagacctggt cggccgggcc gaaggcaacg accccggcgg cgatgaccac
     7441 tccgtcaagt gcggtcagtc ccccgacata gtcgcggatg ctctgcgggt cgctcagatc
     7501 gagtgaagca gtggtgctcc gggatgcgct cagtacagtc gcacccgcgg cgtcgagttc
     7561 ggcgacgagc cgcgaaccaa gacctcccga tgcgccgaca acgagcacca cgcgtccatc
     7621 gagctcagcc atggggcaag cctaggacgc gcacgtcaga caccgaagtg gcggcgcagc
     7681 acccgtgccc acttgttggc aggaaggtcg gggcgca
//
LOCUS       DAOSOU010000086         7668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_9542_length_7668_cov_5.918429, whole genome shotgun sequence.
ACCESSION   DAOSOU010000086 DAOSOU010000000
VERSION     DAOSOU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9542_length_7668_cov_5.918429"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PLW15_06145"
     CDS             <1..849
                     /locus_tag="PLW15_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT34011.1"
                     /translation="PGSRFRSPTGRESTSEQLAASVDTVVRESLGTLPGGGELLGAGI
                     GSAGPITVATGLVSPLNLPSWRDYPLLEHVRALLPGSPVTLRMDGECITLAEHWVGAA
                     QGSQNLMGMVVSTGIGGGLILGGRAVAGPTGNAGHIGHVEVGGYDDPCACGGTGCVEA
                     IASGPKTTAWAQKQGWSGSTGEDLATSYSAGDPIAIAAVTRSGTAIGQAIASATALVD
                     LELVAIGGGFSHVSPDLFDLIRRTVDERAAFPFVTKVRVIPSALSGDGPLIGAAALIH
                     RAALVS"
     gene            complement(842..2080)
                     /locus_tag="PLW15_06150"
     CDS             complement(842..2080)
                     /locus_tag="PLW15_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34012.1"
                     /translation="MTVVATRPLWAGRSLALVGIVLLAFNLRTAVAAVSPIYAVISRD
                     IPLGNLGIGFLGMLPPLAFAASGIVAPAVARRLGLEATLAAACAAMVAGGILRASAGN
                     YATLVAGSVVLLAGMGFGNILLPPVIKRYFPDRIGLMTATYATLMAIGAAIPPLVAAP
                     IAEATSWRISLGTWGLVAVSAVVPWFFLWVRRRADDRASANDGLVEEAAPQLVGRMWR
                     SPVAWAITIAFAVPSFNVYAMFAWLPAIITDLTTLGATEAGGLLALFAIMGLPFALVV
                     PLIAVRVRNVGWMLALGVAFFVTGYLGMLLAPAQATWLWVLLIGSGPIVFPLTLTLIN
                     LRTRTHEASVALSGFVQSIGYGAAALGPLTLGFIHDVTGSWVAPLIFLIGLVLVALIP
                     AFVLARPRMVEDDLARPASN"
     gene            complement(2077..3027)
                     /locus_tag="PLW15_06155"
     CDS             complement(2077..3027)
                     /locus_tag="PLW15_06155"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOT34013.1"
                     /translation="MRIFTSLSDVPADFGPSVVTIGKFDGVHEGHRAMVHRLRTTAAA
                     RGLASVVVTFDRNPLSLLRPEVCPEPLVSTEQKLDLLAATDPHATLVLTFDRSLADET
                     PEEFVRTVLVEPLRAAVVLVGADFRFGAKGAGSVETLIELGATHGFEVLVVDDVTAVD
                     GRRVSSSWIRELLSEGRVAEAARLLGAPHTIRGTVVHGHQRGRQLGYPTANLSREVDG
                     FVPADGVYAAWLTARDIRYPAAVSIGNNPTFEGIVGRQIEAHAIDAAFDIYDEVVDVS
                     FVEYIRGMRKFPGPDELAVQMGADEDQIRTILGVPPKAHQ"
     gene            complement(3024..3440)
                     /locus_tag="PLW15_06160"
     CDS             complement(3024..3440)
                     /locus_tag="PLW15_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34014.1"
                     /translation="MAEKGSTRAIGVGRVLIAVYAVLALGATARSLFQIATKFDDAPV
                     AYLLSGLAGLVYILATVALLNRGRAWYLVAWVTISFELLGVLVIGLLSVIDPVLFPAD
                     TVWSYFGRGYLFIPLLLPIFGILWLVRTRPAVEAAR"
     gene            complement(3482..3850)
                     /locus_tag="PLW15_06165"
     CDS             complement(3482..3850)
                     /locus_tag="PLW15_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34015.1"
                     /translation="MIEWFTTVQLVVAVAAGLLAVVLGLAGRKPNDLVLASAALVELL
                     LIAQIIVAIVAPAVGNAPTGSGLEFWVYLVSAALLPPLAVFWALVERNRWSTVIVGVA
                     CFAVAVMLYRMGQIWFVQVA"
     gene            complement(3847..4779)
                     /gene="truB"
                     /locus_tag="PLW15_06170"
     CDS             complement(3847..4779)
                     /gene="truB"
                     /locus_tag="PLW15_06170"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT34016.1"
                     /translation="MKSAPANPASGILLVDKPQGITSHDVVARARKAVGTRKVGHAGT
                     LDPMATGLLILGVNSATRLLTYIVGLDKQYTATIRLGVATTTDDAEGEPLAEGPAAAL
                     SAVTDDAIIAGAAGFAGTILQRPSSVSAIKVDGRRAYALAREGAPVELAPRSVTVSAF
                     DVLAIRRSEWIDVDVRVDCSSGTYIRALARDLGDLLGVGGHLTALRRTRIGPFRVDAA
                     VGLDEPLAERMLAPAAVAASLHPRLDLTAQEAVDLAHGKRIALAGAGTRAAVAPDGRL
                     VGLVEAVDGKAVVLVNFPPDPTAPSRIETQGEQT"
     gene            4849..5844
                     /locus_tag="PLW15_06175"
     CDS             4849..5844
                     /locus_tag="PLW15_06175"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOT34017.1"
                     /translation="MRIGVIGAGAVGGAIAALLARAGHEVEVTARGAHLDAIRERGIH
                     LTGGWGEYTASVLANETLTRGPELAILATKAQDAVAAIRENVALLRGVPLVVVQNGLD
                     SLTNAHAATPRSDIVGALATYATSYLSPGEIAITTPGPTYLGVEGDNDLPARYAARIL
                     NEVMPTTVIPNFRGAQWTKLVINQVNALPAITGLSVQEVVANRRLRRVMTSSMRECVR
                     IGFASRVHYEKLQGLGPRLLRLFSILPLWAGQALPALMSARIGRTPNPGSTLQSIRRG
                     QASEIDYLNGSVVRAAEARGRRAPVNAALVALVHEVEASGEFFTADAVVARVSGR"
     gene            complement(5883..6539)
                     /locus_tag="PLW15_06180"
     CDS             complement(5883..6539)
                     /locus_tag="PLW15_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOT34018.1"
                     /translation="MAAFTPLKRDVLDSLADEFLHNYGKGRTLLAVDGVDGAGKTHFA
                     DALAERLGRNGHAVFRASIDGFHRPRAERYVKGPDSAEGFYADSFDYDLFRRVLVEPF
                     RLGGSAGFVTAAWDVARDTAVEMAWQTGPQDATLIVDGIFLNRPQLRGLWNYSIWLEV
                     PREIADARLATRDGAPAAQRYLGGQALYFAEADPRARATAIVDNSDFDHPRRVFADSC
                     "
     gene            6576..7442
                     /locus_tag="PLW15_06185"
     CDS             6576..7442
                     /locus_tag="PLW15_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT34019.1"
                     /translation="MNIAPAVNDWFVAHRRDLPWRREGFGAWGILVSEIMLQQTPVVR
                     VVPRLEQWLERWPTPAALAASPPAEAVRAWDRLGYPRRALNLHAAAVAITDRHGGVVP
                     SSVDDLLALPGIGDYTARAVAAFAYGERHPVVDTNVRRVIARAVLGQAVAGPPSTRRD
                     LAAMEALLPESWPDARLFNAGMMELGALVCTARSPRCDECPLAAACAWRAAGYPEYDG
                     PRAAVQKKFEGSDRQVRGLIMAELRASDIPVTALEISTVWPDATQRDRALAGLLADGL
                     VVAVDGGYELPR"
BASE COUNT         1293 a         2611 c         2657 g         1107 t
ORIGIN      
        1 cccggcagcc gttttcggtc accgacgggc cgggaatcga ccagtgagca gctcgccgca
       61 agcgtcgaca cggtcgtgcg cgagagtctg ggcacgctgc ccggcggagg cgagctcctc
      121 ggtgccggaa tcggctcggc cggacccatc accgtcgcga ccggtctcgt gtccccgctc
      181 aaccttccga gctggcggga ctatcccctg ctcgagcacg ttcgcgcact gcttcctggc
      241 agtcccgtga cccttcgcat ggacggcgag tgcatcaccc tcgccgagca ctgggtgggc
      301 gctgcacagg ggtctcagaa cctcatgggc atggtcgtct cgacgggtat cggcggcggc
      361 ctcatcctgg gagggcgcgc cgttgcggga cccaccggaa acgccgggca catcggacac
      421 gtcgaggtcg gcggctacga cgacccctgc gcgtgcggtg gaaccggatg tgtcgaggcg
      481 atcgcgtcag gcccgaagac gaccgcctgg gcgcagaagc aaggctggtc gggcagcacg
      541 ggggaagacc tcgccacaag ctacagtgcc ggcgacccca ttgcgatcgc tgcggtaaca
      601 cgatccggaa ctgccatcgg ccaggcgata gcctccgcca cagcgctcgt cgatctcgag
      661 ctggtggcga tcggtggtgg gttctcgcac gtatctcccg acctgttcga cctgattcgg
      721 cgaacggtcg acgagcgggc cgcgttcccg ttcgtcacca aggtcagggt gatcccctcg
      781 gccctgtcgg gagacggccc gctgatcggc gcggctgcgc tgatccaccg cgcagcactc
      841 gtcagttaga cgcgggccgt gccagatcgt cttcgaccat tcgaggccgc gccagcacga
      901 atgccggaat gagggcgacc agcacgagac cgatcaggaa gatcaagggc gcgacccacg
      961 agcctgtcac gtcatggatg aaccccaagg tcaacggacc caaggccgcc gctccgtagc
     1021 cgatgctctg cacgaatccg ctgagagcga cggatgcctc gtgcgtgcgg gtacgcaggt
     1081 tgatcagcgt gagcgtcagc gggaagacga tcgggccact gccgatgagc agcacccaga
     1141 gccacgtcgc ctgggcgggg gcgaggagca tcccgagata accggtcaca aagaacgcga
     1201 cgcccagtgc cagcatccac cccacattgc gcactctcac cgcgatcagc ggcacgacca
     1261 gggcgaacgg caggcccatg atcgcgaaca gcgctagcag accgcccgcc tcagtcgcgc
     1321 ccagagtcgt gagatcggtg atgatcgcgg gcaaccacgc gaacatcgcg tagacgttga
     1381 aactcggtac cgcgaacgcg atcgtgatcg cccaggccac cggcgagcgc cacatcctgc
     1441 cgaccagctg gggcgccgcc tcctccacca gaccgtcgtt cgccgatgcg cggtcgtcgg
     1501 ccctccgcct gacccagagg aagaaccagg gcacgacggc tgaaaccgcg acgagccccc
     1561 aggtccccag cgagatccgc cagctggtgg cctcggcgat cggggccgcc accagcggcg
     1621 gaatcgcagc cccgatcgcc atgagcgtgg cgtaggtggc cgtcatgagg cctatgcggt
     1681 cggggaaata cctcttgatc accggcggca gcaggatgtt cccaaaaccc atgcccgcga
     1741 gaagcaccac gcttccggcc accagggtgg cgtagtttcc ggcactcgcg cgcagaatgc
     1801 cgcccgccac catagccgcg caggccgcgg caagggtcgc ctcgagtccg agccgtcgtg
     1861 cgaccgccgg ggccacgatg cccgacgccg cgaatgcgag cggaggaagc atgcccagaa
     1921 agccgattcc caggtttccc agggggatgt cccgcgagat caccgcgtag atcggagaca
     1981 cggcggcaac ggcggtgcgc aggttgaagg cgagaagcac gatgcccacc aacgcaagcg
     2041 agcgccccgc ccagagcggg cgggtcgcga ctacggtcac tggtgagctt tcgggggcac
     2101 acccaggatc gttcgaatct gatcctcgtc agcacccatc tgcaccgcga gctcatcggg
     2161 cccggggaac ttgcgcatgc cgcggatgta ctcgacgaac gacacatcga cgacctcgtc
     2221 gtagatgtcg aaggccgcat cgatcgcgtg cgcctcgatc tgccgaccga caatgccctc
     2281 gaatgtcggg ttgttgccga tcgagacggc tgccgggtag cggatgtcgc gcgcagtgag
     2341 ccacgccgca tagaccccgt cggccggcac gaagccgtcg acctcacgcg agaggttggc
     2401 cgtcggatac cccagctggc gaccccgctg gtgcccgtgc acaaccgtgc ctctgatggt
     2461 gtgcggcgca ccgagcagcc gagcggcctc ggcaacgcgc ccctcgctca gcagctcgcg
     2521 gatccagctc gatgacaccc ggcgaccatc gactgccgtc acatcatcga cgaccagcac
     2581 ctcgaaaccg tgcgtcgcac cgagttcgat gagggtctcg acgcttccgg cacccttcgc
     2641 gccgaacctg aagtcggctc cgaccagcac taccgcggct ctgagcggct cgacgagcac
     2701 agtccgcacg aactcctcgg gcgtctcatc cgccaacgat cggtcgaagg tgagcacgag
     2761 ggtcgcgtgt gggtcggtcg ccgccaggag gtcgagcttc tgctcggtgc tgacgagcgg
     2821 ttcgggacac acctcgggcc gcagtagcga cagcgggttg cgatcgaagg tgacgacgac
     2881 cgacgcgagt ccgcgtgcgg ccgccgtggt gcgcaggcgg tgcaccatcg cccgatgtcc
     2941 ctcgtgcacg ccgtcgaact tgccgatcgt cacaacgctc ggaccgaagt cggcaggaac
     3001 atccgagagg ctggtgaaga tcctcatcgg gctgcctcga ctgcgggtcg ggtgcgcacg
     3061 agccagagaa tcccgaagat gggcagcagg agcgggatga acagatagcc gcgcccgaag
     3121 tacgaccaca cggtgtcggc cgggaagagc acggggtcga tcacgctgag cagcccgatc
     3181 accagcacac cgagcaactc gaagctgatg gtgacccagg caaccaggta ccaggcacgg
     3241 ccgcgattga ggagggccac cgtggcgagg atgtagacca ggccggccag cccggagagc
     3301 aggtaggcga ccggtgcatc atcgaacttc gtcgcgatct ggaaaagaga ccgtgcagtc
     3361 gcgccgaggg cgagcaccgc gtacacggca atcagcacac gaccgacgcc gatcgcgcgg
     3421 gtggaacctt tctcagccat cgggtcaact ttaggcgact cgacggcctg cacttccccg
     3481 ctcacgcgac ctggacgaac cagatctggc ccatacggta aagcatcacc gcgacggcga
     3541 aacaggcgac gccgacgatg acggtgctcc agcggttgcg ctccacgagc gcccagaaga
     3601 cagcgagcgg cggcagcagc gccgccgaga ccaggtagac ccagaactcc agaccgctgc
     3661 cggtcggcgc attgccgacg gcaggggcga cgatggccac gatgatctgc gcgatcagca
     3721 gaagctcgac cagtgccgcg gatgcgagca cgagatcatt gggtttgcga ccggccaggc
     3781 cgagcacgac ggccagaagc ccggcggcga cggcgaccac cagttgcacc gtcgtgaacc
     3841 actcgatcat gtctgctctc cctgggtttc gattctgctc ggcgctgttg ggtccggcgg
     3901 aaagttcacg aggacgacgg ccttgccgtc caccgcttcg acgaggccga cgaggcgacc
     3961 gtccggggca accgccgctc gcgttcccgc gccggcgagc gcgatccgct tgccgtgggc
     4021 gaggtcgacg gcctcctggg cggtcaagtc gagtcggggg tgaagggatg ccgcgacggc
     4081 ggccggggcg agcatcctct cggccaacgg ctcgtccagc ccgaccgcgg catccactcg
     4141 gaacgggcca atgcgtgtgc gccgcagggc cgtcaggtga ccgccgactc ccagcaggtc
     4201 ccccaggtcg cgggcgagcg cgcgaatgta cgtgcccgag ctgcaatcca cgcgaacatc
     4261 aacgtcgatc cactccgatc gacggatggc cagcacgtcg aaggcggaga ccgtcacgct
     4321 gcgcggggca agctcgaccg gcgcgccctc gcgagccagg gcataggcgc gacggccatc
     4381 caccttgatc gcgctgacgc tgctcgggcg ctgaaggatg gtgccggcga atcctgccgc
     4441 gcccgcgatg atcgcgtcgt cggtgacggc gctgagcgcc gcagcggggc cctccgcgag
     4501 cggctcgccc tcggcgtcat ccgtggtcgt cgcaacgccc agcctgatcg tggccgtgta
     4561 ctgcttgtcg agcccgacga tgtaggtcag caggcgcgtc gccgagttca cgccgaggat
     4621 cagcaggccg gtcgccatcg ggtcgagagt tcccgcgtgg ccgaccttgc gggtgccgac
     4681 agccttgcgg gcacgggcga ccacgtcgtg gctggtgatg ccctgcggtt tgtcgacgag
     4741 caggatgccg ctggcggggt tggccggtgc ggacttcact cttgcgatcc taacggggcc
     4801 cgggcgcgcg tcccgggcgg tcggggtatg cgcccctagt atctgagcat gcggatcggc
     4861 gtcatcggag cgggagcggt cggtggcgcc atcgcggcac tgctcgcgcg ggccggccac
     4921 gaggtcgagg tgacggcgcg cggcgcgcac ctcgacgcga tacgggagcg gggcatccac
     4981 ctcaccggcg gctggggcga gtacacggcc agcgttctcg ccaacgagac tctgacccgc
     5041 ggacccgagc tggccatcct ggcgacgaag gctcaggatg ccgtggccgc gatccgcgag
     5101 aacgtcgcgc ttctgagagg ggtccccctc gtcgtcgtgc agaacggact cgacagcctc
     5161 accaatgcgc acgccgccac cccgcgcagc gacatcgtgg gagccctcgc gacctacgcc
     5221 acctcgtacc tctcgcccgg cgagatcgcc atcaccacgc ccggtccgac ctatctcggc
     5281 gtcgaaggcg acaatgacct gcccgcccgg tatgcggcgc gcatcctcaa cgaggtgatg
     5341 ccgacgaccg tcatccccaa cttccgtggc gcccagtgga ccaagctcgt gatcaaccag
     5401 gtcaacgcac tgcccgcgat caccgggctc agcgtgcagg aggtggtggc caatcggcgc
     5461 ctgcgccggg tgatgacctc gagcatgcgg gagtgcgtgc gcatcggatt cgcgagcagg
     5521 gtgcactacg agaagctcca gggactcggc cccagactgc ttcgattgtt ctcgatcctg
     5581 ccgttgtggg ccggtcaggc actccccgca ctcatgagcg cacgaatcgg gcgcaccccc
     5641 aaccccgggt cgacactgca gagcatcagg cgcggacaag ccagcgagat cgactacctc
     5701 aacgggtctg tggtgcgggc ggccgaggcg cgcggtcggc gggcgccggt caacgccgcg
     5761 ctggttgcac tcgtgcacga ggtcgaggca tcgggcgagt tcttcaccgc ggatgcggtg
     5821 gtggcacggg tgagcgggcg gtagcgcgcg gtttcgatac gcgctgcgcg ctactcaacc
     5881 agctagcagc tgtctgcgaa gaccctccgg gggtggtcga agtcggagtt gtcgacgatg
     5941 gccgtggctc tggcccgtgg atccgcctcg gcgaagtaca gcgcctgccc gccgaggtag
     6001 cgttgcgcgg cgggggctcc gtcgcgggtg gcgagccggg catccgcgat ctctcggggc
     6061 acctcgagcc agatcgagta gttccacaga ccgcgaagtt gcggacggtt gaggaagatg
     6121 ccgtcgacaa tgagcgtggc atcctgcggt ccggtctgcc acgccatctc gaccgcggtg
     6181 tcgcgcgcga cgtcccaggc ggcggtcacg aaacccgcgc ttccgccgag gcggaacggc
     6241 tcgacgagca cgcggcggaa caggtcgtag tcgaacgagt cggcgtagaa gccctcggcc
     6301 gagtccgggc ccttcacgta gcgttcggcg cgcggtcggt ggaacccgtc gatggatgcc
     6361 cggaacacgg catgaccgtt gcgacccagt cgttcggcca gggcatccgc gaagtgggtc
     6421 ttgcccgcac cgtcgacacc gtcgaccgcc agcagggtgc gtcccttgcc gtagttgtgc
     6481 aggaactcgt cggcgagcga gtcgagcacg tcacgcttga gcggtgtgaa ggcggccatg
     6541 cgcccagtct agatttgccc gtctaggttt gactcgtgaa tatcgccccc gccgtcaacg
     6601 actggttcgt cgcccaccgg cgcgacctgc cgtggcgtcg ggaggggttc ggcgcgtggg
     6661 gcatcctcgt gagcgagatc atgctgcagc agacgccggt cgtgcgggtg gtgccgcggc
     6721 tcgagcagtg gctggagcgc tggccgactc cggccgcgct ggcggcctct cccccggccg
     6781 aggccgtgcg cgcgtgggat cgtctcggct atccgcgccg cgcgctcaac ctgcatgccg
     6841 cggcggtggc gatcacggat cggcacggtg gggtcgtacc ctcctcggtg gatgacctgc
     6901 tcgccctgcc cggaatcggc gactacaccg cccgggccgt ggccgccttc gcgtacggcg
     6961 agcggcatcc ggtcgtcgac acgaacgtgc ggcgcgtcat cgcccgggcg gtgctggggc
     7021 aggcggtggc cgggccgcca tccacccgac gagacctcgc cgcgatggag gcgctgctgc
     7081 ccgagtcgtg gccggatgcc cggctcttca acgccggaat gatggagctc ggcgccctcg
     7141 tctgcaccgc gcggtcaccc cgctgcgatg aatgccccct cgccgcggcg tgcgcgtggc
     7201 gggccgccgg gtatcccgag tacgacggac ctcgggcggc ggtgcagaag aagttcgagg
     7261 gcagcgaccg tcaggtgcgc ggcctcatca tggccgagct gcgtgccagc gacatcccgg
     7321 tgacggctct tgagatctcg accgtgtggc cggatgcgac gcagcgcgac cgcgctctcg
     7381 ccgggctgct tgccgacggg ctggtcgtcg cggtggatgg cgggtacgag ttgccgcggt
     7441 aggtaggact cgaacgtacg ttcgaaagaa tgtcggtggc atggtgtgaa gtggaaccat
     7501 gagattcgag gcagagcagt ccggcggggg cgccgcgctt gctacgactg cgctcgctcc
     7561 gaccgcgagc ctgcacggcg ccatggcgaa cacgctcggt gacctcatcg acggcatcgc
     7621 cgcgctcgac cgcatggagg cgtcgctcgc cgcgtacaag gccgagct
//
LOCUS       DAOSOU010000087         7549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_9749_length_7549_cov_5.499466, whole genome shotgun sequence.
ACCESSION   DAOSOU010000087 DAOSOU010000000
VERSION     DAOSOU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9749_length_7549_cov_5.499466"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="PLW15_06190"
     CDS             complement(<1..1871)
                     /locus_tag="PLW15_06190"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOT34020.1"
                     /translation="MFTKILVANRGEIAIRAMRAAFELGAKTVAVFPYEDRNSMHRLK
                     ADEAYQIGEKGHPVRAYLDVSEIIRVAKESGADAIYPGYGFLSENPDLAQAAADAGIT
                     FIGPPTRVLEMAGNKVTAKEHAIAAGVPVLKSTPASKDIEELLAGAAEIGFPIFAKAV
                     AGGGGRGMRRVDKAEDLREALEAAMREADSAFGDPTMFLEQAVVRPRHIEVQILADSQ
                     GETVHLFERDCSVQRRHQKVIEIAPAPNLDETTRQAMYRDAIAFAKSIGYVNAGTVEF
                     LLDTAGERKGQHVFIEMNPRIQVEHTVTEEVTDVDLVQSQMRIAFGQSLKELGLQQEN
                     LHLRGAALQCRITTEDPAAGFRPDTGKITTYRSPGGAGIRLDGGTVAAGAQVSPHFDS
                     MLVKMTCRGRDFAAAVVRARRGLAEFRLRGVTTNIPFLQAVLDDPDFIAGDLSTSFIE
                     ERPWLVRGRQSKDRGTKVLNWLADVTVNRPYGDGAGLISPALKLPVVDLDTPAPAGSR
                     QRLLELGPVGFAAALRQQTPLAVTETTFRDAHQSLLATRVRTKDLVAVLPHVARLTPE
                     LLSIEAWGGATYDVALRFLGEDPWERLGAMREALPNVALQMLLRGRNTVGYTPYPTEV
                     "
     gene            1993..2802
                     /locus_tag="PLW15_06195"
     CDS             1993..2802
                     /locus_tag="PLW15_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT34021.1"
                     /translation="MHVLSVSSLKGGVGKTTVTLGLASAAFAKGLRTLVVDLDPQSDV
                     STGMDIEVAGHLNVADVLASPKEKIVRAAIAPSGWTKGRPGKVDVLIGSPSAINFDGP
                     HPSIRDIWKLEEALANVEADYDLVLIDCAPSLNALTRTAWAASDRVTIVTEPGLFSVA
                     AADRALRAIEEIRRGLSPRLQPLGIIVNRLRSQSLEHQFRIKELRDMFGPLVLSPQLP
                     ERTSLQQAQGAAKPLHVWPGESAQEMARNFDQLLERVMRAARIGEFANDPV"
     gene            complement(3114..3662)
                     /locus_tag="PLW15_06200"
     CDS             complement(3114..3662)
                     /locus_tag="PLW15_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT34022.1"
                     /translation="MSELSRSEEPRYDDGLLFTDGLPDLDDAAGYRGAIAARAAGISY
                     RQLDYWARTGLVEPTVRGAAGSGSQRLYGFRDILVLKLVKRLLDTGISLQQIRVAVNQ
                     LRESGINDLAQTTLMSDGASVYLCTSSDEVIDLVSRGQGVFGIAVGKVLREVEHDLVE
                     LDSVPGEDPMDELARRRAVKAS"
     gene            complement(3788..4486)
                     /locus_tag="PLW15_06205"
     CDS             complement(3788..4486)
                     /locus_tag="PLW15_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT34023.1"
                     /translation="MARVASQARVPGGPALLSIGQVLAKLNPEFPDLTPSKLRFLEER
                     QLVAPARTDSGYRKFSPSDMDRLRFVLTMQRDHYLPLKVIRGYLAELDAGRTPELPGG
                     AVASPSMLSSERRFTREELIRESGANAMLLGDAISASLIMPADVFGEDSLAVLKALVE
                     LQRSGIEPRHLRGFRAAMERELGLIENALMPVSRRKDASSRARAAEMAREIAGQLEIV
                     RSSLIRSALTRLDS"
     gene            complement(4486..4986)
                     /locus_tag="PLW15_06210"
     CDS             complement(4486..4986)
                     /locus_tag="PLW15_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT34024.1"
                     /translation="MVDSGNLGHAADDVPPSGASAGGPNDDATVHYGDEFAAQLAALD
                     GGVSSEEVDAIAALPSGSALLVVRRGPNVGARFLLDADVTTVGRHPEADIFLDDVTVS
                     RRHAEFLRHGTAFQVKDLGSLNGTYFDGVRIDTALLSDGAEVQVGKFRLTFYASRYDL
                     AHLAGR"
     gene            complement(4998..5615)
                     /locus_tag="PLW15_06215"
     CDS             complement(4998..5615)
                     /locus_tag="PLW15_06215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT34025.1"
                     /translation="MGAIKGSEVSGRVLTVPNLLSFVRLAMVPVFLVLVLDGQDALAL
                     LVLVISSLTDFLDGYIARTFKQISRLGQLLDPAADRLFIFAALIGLAARGVIPWWLFA
                     AIVGRDILLLILGIVAANFGYGPLPVHHLGKVATACLFYALPILMLGQAFPDAAWLTN
                     PLGWAFALWGAFLYWWAGAIYLIETSRLLRIPRVEPATESDTLGS"
     gene            5660..6469
                     /locus_tag="PLW15_06220"
     CDS             5660..6469
                     /locus_tag="PLW15_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOT34026.1"
                     /translation="MTPLVLVLIVAGVVCAFVWITSLITKEHSWVDRLWSIVPVVYVW
                     IFAAFAGLADARLTIMAILVTLWGARLTFNFARKGGYSGTEDYRWPILRSRMKGWQFE
                     IFNLLFIVLYQNLILVLIALPALTAYEHQGGFGVPDVVVALLFLGLLIGETVADQQQW
                     VFQNAKKAGGADFRPRFLTTGLWRFSRHPNFFFEQAQWWALFLFGAIAAGSLLQWTVL
                     GAVLLTILFIGSTRFTEEITRSKYPEYADYQATTSPIVPWPPRRKESAVTA"
     gene            6490..6933
                     /locus_tag="PLW15_06225"
     CDS             6490..6933
                     /locus_tag="PLW15_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34027.1"
                     /translation="MSCIRFNTPAQLAQMRAMAPAMLTAEQAAVLHPSPVMTESKIAR
                     LRLLATDPNPKIRERVALDSHTPTDVFEALARDSDDGVRACVARNENTPCDVLRSLVT
                     DPSETVRGFLAVNFFVPEDAMARLAEDRSRTVRELVRWKTELASA"
     gene            7026..>7549
                     /locus_tag="PLW15_06230"
     CDS             7026..>7549
                     /locus_tag="PLW15_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34028.1"
                     /translation="MTAAVASVLLALTVLAPGQPVSAADVPTWDEIEAARSSESATAA
                     TIATLQAVLDDLQNQAAALGDEAVARRTEADVAQAALVTATTRANALGRRADDAAARS
                     TQSATRAAQIAAALYRSGSRDLTSALLLDGSSGDLLDRLGALDQVGGTVVDVLAGARF
                     DLNEATALADQAALA"
BASE COUNT         1261 a         2519 c         2554 g         1215 t
ORIGIN      
        1 acctcggtgg ggtacggggt gtagcccacg gtgttgcggc cgcgcagcag catctggaga
       61 gcgacgttgg gcagcgcctc gcgcatcgca cccagacgtt cccaggggtc ctcaccgagg
      121 aagcgcagag cgacgtcgta ggtcgcgcca ccccacgcct cgatcgacag cagctcgggc
      181 gtgaggcgcg ccacgtgcgg cagcacggcg acgagatcct tggtgcgaac gcgggtcgcc
      241 agaagcgact ggtgggcgtc gcggaaggtg gtctcggtga cggcaagcgg agtctgctgg
      301 cgcagggcgg ccgcgaagcc caccggcccg agttcgagga gccgctgacg cgagcccgcg
      361 ggagcgggag tgtcgaggtc caccaccgga agcttcaggg cggggctgat gaggccagca
      421 ccgtcgccgt acgggcggtt gaccgtgaca tccgcgagcc agttgagcac cttggtgccg
      481 cgatccttcg actggcgtcc gcgcaccagc caggggcgct cctcgatgaa ggaggtgctg
      541 aggtcgccag cgatgaagtc agggtcgtcg agcacggcct ggaggaacgg gatgttggtc
      601 gtcacgccgc gcaggcggaa ctcggcgaga ccgcggcgcg cgcgcacgac agccgccgcg
      661 aagtcgcggc cgcggcaggt catcttgacc agcatcgagt cgaagtgcgg agagacctgg
      721 gcgccggccg cgacggtgcc accgtcgagg cggatacccg cgccgcccgg cgagcggtag
      781 gtcgtgatct tgcccgtgtc cgggcggaat cccgctgccg ggtcctcggt cgtgatccgg
      841 cactgcaggg cggcaccgcg caggtgcagg ttctcctgct gcagtcccag ctccttcagc
      901 gactgcccga aggcgatgcg catctgcgac tgcacgaggt cgacgtcggt gacctcctcg
      961 gtgaccgtgt gctcgacctg gatgcgcggg ttcatctcga tgaagacgtg ctggcccttg
     1021 cgctcccccg ccgtgtcgag gaggaactcg acggtgccgg cgttgacgta gccgatcgac
     1081 ttggcgaagg cgatcgcatc ccggtacatc gcctgtcggg tggtctcgtc gaggttgggc
     1141 gccggcgcga tctcgatgac cttctggtga cggcgctgca ccgagcagtc gcgctcgaac
     1201 aggtgcacgg tctcgccctg cgagtcggcg aggatctgca cctcgatgtg ccggggtcgc
     1261 acgaccgcct gctcgaggaa catggtgggg tcgccgaacg cgctgtcggc ctcgcgcatc
     1321 gccgcttcaa gcgcctcgcg caggtcctcc gccttgtcga cgcgacgcat gccccggccg
     1381 ccaccgccgg cgacggcctt cgcgaagatg gggaagccga tctcggccgc ccccgcgaga
     1441 agctcctcga tgtccttcga ggcgggggtc gacttgagca cgggaacacc cgcggcaatg
     1501 gcgtgctcct tggccgtgac cttgttgccc gccatctcca gcacgcgggt cggcggcccg
     1561 atgaaggtga taccggcgtc ggccgcagcc tgggcgagat cggggttctc cgacaggaag
     1621 ccgtaccccg ggtagatcgc gtcggcgccc gactccttgg ccacacggat gatctccgag
     1681 acatcgaggt aggcccgaac cggatgcccc ttctcgccga tctggtacgc ctcgtcggcc
     1741 ttcagccgat gcatggagtt gcgatcctcg tacgggaaca ccgccaccgt cttggcaccc
     1801 agttcgaatg ctgcgcgcat cgctcgaatg gcgatctcac cgcggttggc tacgagaatc
     1861 ttcgtgaaca tccggtcctt tcgtacgtgc gtcaagccta gggcgcgcga acactggcaa
     1921 ttcacccagc agggcctgat cggaggtcag gatttaggtt ctttaagagt agtgaaggta
     1981 tcgtcgttac tcgtgcatgt actcagcgtc agctccctca aggggggcgt gggaaagacc
     2041 accgtgacgc tcgggctcgc ctcggctgca ttcgccaaag gcttgcggac tctcgtggtc
     2101 gatctcgacc cacaatccga cgtgtcgacg ggtatggaca tcgaggtcgc aggtcacctc
     2161 aatgtcgcgg atgttctcgc ctcccccaag gagaagatcg tgcgcgccgc gatcgccccg
     2221 agcggttgga ccaagggtcg tcccggcaag gtggacgtgc tcatcggatc gcccagcgcg
     2281 atcaatttcg acgggccgca ccccagcatc cgggacatct ggaaactcga ggaggctctc
     2341 gccaacgtcg aggcagacta cgacctcgtg ctcatcgact gtgcgccctc tctgaacgcg
     2401 ctcactcgca ccgcgtgggc cgcgagcgac cgggtgacga tcgtgaccga acccggcctc
     2461 ttctccgttg ctgccgccga ccgtgccctg cgcgcgatcg aggagatccg ccgcggactc
     2521 tcgccccggc ttcagccgct cggcataatc gtgaaccggc tgcggtcgca gtcgctcgag
     2581 caccagttcc gcatcaagga gttgcgcgac atgttcgggc cgctcgtgtt gagcccgcag
     2641 ctgccggagc gcacgtcgct gcagcaggcg cagggtgccg cgaagccgct gcacgtgtgg
     2701 cccggcgaga gtgcgcagga gatggcccgc aacttcgacc agcttctgga acgagtcatg
     2761 cgggcggcgc gcatcggcga attcgccaac gaccccgtct agcgacgctg cctacgggct
     2821 gcccagcgcg gctgcccagc gcggctccgg tcccgccact gctcccgtag ccgctgctcc
     2881 cttcaccttc tcgcggatca ggagatccgc gcaacacgcc gcccgtcgaa cttgtcgagc
     2941 ggcgcgtccg ggccgatctc ctgatccgcg aacgaggaag aaccgggagg aacggggaag
     3001 aacggggaag aaccgggaag agccggaaag agcagggaag cgggatcggc gagcagcgcg
     3061 gcgatgccgg ctagcggcga cggcgcggat gcccggcgcg aggcatcccc gggctaggag
     3121 gccttgacgg cccgacgacg agccagctcg tccatcgggt cttcgccggg aaccgagtcg
     3181 agctcaacca ggtcgtgctc gacctcgcgc agaaccttgc ccacggcgat gccgaagacg
     3241 ccctgaccgc ggctgacgag gtcgatgacc tcgtcgctcg aggtgcacag gtagacgctc
     3301 gcaccatcgc tcatgagcgt ggtctgggcc agatcgttga tgcccgactc gcgcagctgg
     3361 ttcacggcaa cgcggatctg ctgcagcgag attccggtgt cgagcagacg cttgacgagc
     3421 ttgagcacga ggatgtcgcg gaagccgtag aggcgctgcg accccgagcc ggcagctccg
     3481 cgcaccgtgg gctccacgag tccggtgcgg gcccagtagt ccagctggcg gtagctgatg
     3541 ccggccgcgc gggccgcgat cgcgccgcgg taaccggcag cgtcatccag atcgggaaga
     3601 ccgtcggtga agagcaggcc gtcatcgtag cggggttcct cgctacggct gagttcgctc
     3661 atggccttgc ccctctaacc ttcatgttga agttgacact tagtgttgcg tgcaacccca
     3721 cggtactcac gcccgagtgc cgatgcccgt catcggccgg gcgtgtcgcg acgtgagttc
     3781 gacggtacta cgaatcgagc cgtgtgagtg cggatcggat cagactgctg cgaacaattt
     3841 cgagttggcc ggcgatctcc cgggccatct ccgctgcacg ggcacggctc gaggcatcct
     3901 tgcgccgcga cacgggcatg agggcgttct caatgagtcc gagttcgcgc tccatcgccg
     3961 cacgaaaacc gcgcaggtgt cgcggttcga tgcccgaacg ctgcaactcg acgagtgcct
     4021 tgagcaccgc gagcgaatct tcaccgaaga catcggcggg catgatgagc gaagcgctga
     4081 tcgcatcccc caacagcatg gcgttggcac ccgattcgcg gataagctcc tcgcgggtga
     4141 agcgtcgttc cgacgacagc atcgacggcg acgcgacggc tccgcccggc agctcgggag
     4201 tacgcccggc gtcgagctca gcaaggtagc cgcgaatgac cttcagcggc aggtagtgat
     4261 cgcgctgcat ggtgagcacg aaccgcagcc gatccatgtc ggagggcgag aacttgcggt
     4321 agcccgagtc ggtgcgtgcg ggggcgacca gctgccgttc ctccaggaag cgcagcttcg
     4381 agggggtgag gtcgggaaac tccgggttca gtttcgcgag cacctgcccg atgctgagaa
     4441 gggcgggacc cccaggcacc cgtgcctggg aagcaacgcg cgccactacc ggccagccag
     4501 gtgcgcgaga tcgtagcgcg acgcgtagaa cgtcaggcgg aacttgccga cctgcacctc
     4561 ggcgccgtcg ctgagcagag cggtatcgat gcgaacaccg tcgaagtagg tgccgttgag
     4621 ggatccgaga tccttcacct ggaaagccgt gccgtgccgc aggaactcgg cgtgacgtcg
     4681 tgacacagtc acgtcgtcga ggaagatgtc agcttcggga tgccgcccca ccgtggtcac
     4741 atcggcgtcg agcaggaagc gcgcgcccac gttgggtccg cggcgcacca ccagcagcgc
     4801 cgaaccggag ggcagagcag caatcgcgtc aacctcttcg ctcgacactc ccccgtcgag
     4861 cgctgccagt tgcgcggcga actcatcgcc gtagtgcacc gtcgcgtcgt cgttgggccc
     4921 cccagccgac gcaccgctcg gaggcacatc atcggcggcg tgacccaaat tcccggaatc
     4981 aaccatcgcg atcctcccta gcttcccagc gtatctgatt cggtggccgg ctcgacccgg
     5041 ggaattctca gaagtcgact cgtttctatc agatagatcg cgcccgccca ccagtacagg
     5101 aaggcacccc agagcgcgaa ggcccagccg agcgggttgg tgagccacgc ggcatccggg
     5161 aacgcctgac ccagcatgag gatcggcaac gcgtagaaca ggcaggcggt ggcaactttg
     5221 ccgaggtggt gaaccggcag cgggccgtag ccgaagttgg cggccacgat gccgaggatc
     5281 agcagcagga tgtcgcgccc gacgatcgcc gcgaacagcc accacggaat cacgccacgg
     5341 gccgcgagcc cgatcagcgc cgcgaagatg aagagccgat cggccgcagg atcgagcagt
     5401 tgcccgaggc gactgatctg cttgaaggtg cgggcgatgt acccgtcgag gaagtcggtg
     5461 aggctggaga tcaccagcac gagcagcgcg agggcatcct gtccgtcgag cacgagcacc
     5521 aggaagaccg gaaccatggc cagccgtacg aagctgagca gattgggcac ggtgagcact
     5581 cggcccgaca cttcgctgcc cttgattgct cccacgagtg caaagtctag tgacgcgtca
     5641 gaagcaatag gatgcctcaa tgacccctct cgttctcgtc ctcatcgttg cgggcgtcgt
     5701 ctgcgccttc gtctggatca cctcgctcat caccaaggag cactcctggg tcgaccgtct
     5761 gtggtcgata gttcccgtcg tctacgtgtg gatcttcgcc gccttcgctg gcctcgcgga
     5821 tgcccggctc accatcatgg cgatcctcgt gactctctgg ggcgcgcgcc tgacgttcaa
     5881 cttcgcgcgc aagggcggat actccggcac cgaggattac cgctggccga tcctgcgctc
     5941 gcgcatgaag ggctggcagt tcgagatctt caacctgctc ttcatcgtgc tgtaccagaa
     6001 cctgatcctc gtgctgatcg ctctgcccgc gctcacggcc tacgagcacc agggcggctt
     6061 cggcgtgccc gacgtcgtcg tggcgctgct gttcctgggt ctgctgatcg gtgagacggt
     6121 cgccgaccag cagcagtggg tgttccagaa cgcgaagaag gcgggcgggg cggacttcag
     6181 gccgcggttc ctgacgacgg gcttgtggcg cttctcgcgt catccgaact tcttcttcga
     6241 gcaggcccag tggtgggcgc tgttcctgtt cggcgcgatt gcggccgggt cgctgctgca
     6301 gtggaccgtg ctgggcgcgg tgctgctcac gatcctgttc atcggctcga cgcgcttcac
     6361 cgaggagatc acgcgctcga agtaccccga gtacgcggac taccaggcga ccacctcgcc
     6421 gatcgtgccg tggccgccgc gccgcaagga atcggccgtc acggcctagc ctccgtttag
     6481 cgttgtcgca tgtcgtgcat ccgcttcaac accccggccc agctcgcgca gatgagggcc
     6541 atggcgcccg cgatgctgac tgcggagcag gccgcggtgc tgcatccgtc gccggtgatg
     6601 accgagagca agatcgcgcg actgcgcctg ttggccactg atccgaaccc gaagatccgt
     6661 gagcgggtcg ccctcgacag ccacaccccg accgacgtgt tcgaggcgct cgcccgggac
     6721 tcggacgacg gggtgcgtgc ctgcgtggcg cgcaacgaga acaccccgtg cgacgtgctg
     6781 cgctcgctcg tcaccgaccc gtcggagacc gtgcggggct ttctcgcagt gaacttcttc
     6841 gtgcccgagg atgccatggc gcgcctcgcc gaggaccgca gccgcacggt gcgggagctc
     6901 gtgcgctgga agaccgagct ggcctcggcc tagttgatcc cacgagtcac accagtcaca
     6961 ccattcactt tctcaacgga agtgcctagc cttgaggtat gggcgagcga cgaaggactg
     7021 tgacggtgac ggccgccgtc gccagtgtgc tgctcgcgct caccgtgctc gcaccgggcc
     7081 agcccgtttc tgccgctgac gtaccgacct gggacgagat cgaggcggcg cgcagttccg
     7141 aatccgcgac ggcggccacg atcgcgaccc tgcaggccgt gctcgacgat ctgcagaacc
     7201 aggcggccgc tctcggcgac gaggcggtcg cgcggcgcac cgaggcggac gtcgcgcagg
     7261 ccgccctcgt gaccgcgacc acgcgcgcga acgccctcgg ccgtcgagcg gatgacgcag
     7321 ccgcacgctc gacccagtcc gcgacccggg ccgcccagat cgccgcggcg ctctaccgct
     7381 ccggtagccg ggacctgacg tcggcgttgc tgctggacgg gtcatccgga gacctgctcg
     7441 accgtctggg ggcgctcgac caggtcggcg gcaccgtcgt cgatgtgctg gctggcgctc
     7501 gattcgatct gaacgaggcg accgcactcg ccgaccaggc ggcactcgc
//
LOCUS       DAOSOU010000088         7516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_9801_length_7516_cov_5.411339, whole genome shotgun sequence.
ACCESSION   DAOSOU010000088 DAOSOU010000000
VERSION     DAOSOU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9801_length_7516_cov_5.411339"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLW15_06235"
     CDS             <1..774
                     /locus_tag="PLW15_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT34029.1"
                     /translation="AIVAIIALVVVNSIKPAGPGPRNMASDGIQLSQGAIATETPGLK
                     PGEDPVPNVRDEASGVLDIQMYVDYLCPICGQFEATNGEYISSLLENGGTTVEIHPIA
                     ILDRLSQGTKYSTRAVNAAACVADMSPNQFYDFHNQLFANQPAENTTGLTDEEIIALT
                     VAAKVDNADKIASCIKDQSFKAWATAATARALNGPIPNSNVDKVTGTPTVIVNGLKYE
                     GAVNDLASFQAFVLQAAGSDFNNSSTSTPTPTPSPTPAG"
     gene            complement(835..1884)
                     /locus_tag="PLW15_06240"
     CDS             complement(835..1884)
                     /locus_tag="PLW15_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT34030.1"
                     /translation="MPLLQSLRNPGVQAGIASAVLFGAGTPLAKLLLGSVSPWMLAGL
                     LYVGSGIGLGLYRFVTRPERVRLKRAEVLPLVGAIGFGGILGPVLLMFGLSGMPATGA
                     SLLLNAEGLFTAVIAWVVFRENVDRRIALGMLAIVAGAVVLSIPTGADLGPVLPSLAI
                     LGACLCWGIDNNLTRKIALTDATWLAAVKGGVAGPVNLVIAFALGAQLPPAASVGAAM
                     LVGLGAYGVSLVLFIVAMRHLGAARAGAYFSVAPFFGAALAIALGASVTVPLIIAAAL
                     MALGVWLHLTERHVHEHTHLAVTHDHWHAHDEHHQHEHDAAVEPGIRHRHVHTHDALT
                     HTHEHYPDAHHRHDH"
     gene            complement(1920..2312)
                     /locus_tag="PLW15_06245"
     CDS             complement(1920..2312)
                     /locus_tag="PLW15_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34031.1"
                     /translation="MTDTGTKDDPWELVTAPGSSNYTMYIDGDELVCQVGSTTLKYQA
                     RAIEDLTAWLKEQGDWVQLGAADESKAAADGTVEAWGRDASNPVGGWYGLRKGYRGRF
                     GMYLPPLLEKLGLVELTHDARNNSVRAL"
     gene            2493..3488
                     /locus_tag="PLW15_06250"
     CDS             2493..3488
                     /locus_tag="PLW15_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene cyclase"
                     /protein_id="HOT34032.1"
                     /translation="MAIDPALLNWLLDSDPALRWRVERDLAGAPRETWAATRESIATE
                     GFGARLLELQDADGQWAGGSFFPKDFDFNGPEAAEGAGQPWTATTWTLNTLREWGLDA
                     SALEGTAELLDANSRWDYNDLPYWGGEVDCCINSYTLANGAWLGADVAGIAQWFLDHR
                     MDDGGWNCEWENGSTRSSFHSTLNTLIALLYYEEVTGGSDALREARRAGEEYLLQRRL
                     MRRLTTGEIVGPWVTRFAYPFRHYYGVLRAVDYFRSATLFDGTEPDERITDAVEAIRA
                     ARRPDGTWLQERRHPGRVWFEVDAPVGEPSKWLTYYGTRSLDWWDNGSVSVIPHV"
     gene            complement(3498..4304)
                     /locus_tag="PLW15_06255"
     CDS             complement(3498..4304)
                     /locus_tag="PLW15_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HOT34033.1"
                     /translation="MSLDLFFAEAYLALWLFFIYSFAGVIVEMIYCWTIEYKGVIESR
                     LGLLYLPFNLLYGTGGVIITLVLVPYFDNPLIIFGLGLVVGTVLEYVTSLVMEKVFHA
                     VYWDYSKEFLNIQGRVCLKYAIFWGLLSVFLLYVLDVINLVLILSIPVIPGVIALVVL
                     IVLTLASITLTLLAYRRVEQKNAYLEAKKAGQDATLPSPAWGRLVDRLVPDVVLVNTF
                     PRMSLITQYQELSGRKRKLIVWNPHIGKTTALRREAGERAERHAQSAAGA"
     gene            complement(4349..6580)
                     /locus_tag="PLW15_06260"
     CDS             complement(4349..6580)
                     /locus_tag="PLW15_06260"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT34034.1"
                     /translation="MTTVSDVDLDIGGMTCASCATRIERKLNRIDGVTATVNYATEKA
                     RVTADGVDAATLIAAVESAGYSASVPRPVADVAEASEAAGNTEVSALRQRLIVSAVLA
                     APVAALSMIPALQFTNWQWLALALASPVAIWGAWPFHRVAAINARHGAASMDTLISVG
                     VLAAFGWSLYALFFGTAGVAGMHMSFTFFGSPATGSSEIYLEVASAVTVFILAGRYIE
                     VRAKRDSGAALRALLEVGAKNATLRRGEVETLVPASTLVPGDQVVVRPGEKFPSDGLV
                     LDGASAVDASMLTGESIPVEVGIDSRVVGGTLNVGGRLLVEITRVGADTELARIARLV
                     EDAQTGKAQVQRLADRVAGIFVPVVLVLAALTLIAWLALGAAPEIAFTAAVATLIIAC
                     PCALGLATPTALLVGTGRGSQLGILIRGPQVLEMTRRVDTVVLDKTGTVTTGIMSVVH
                     ALPAAGVTEAELVRLAAAVEEGSEHPVARAIAAFAPEVPSPAAEFRAHAGLGVQGIVD
                     GRAVVAGKSSWLTESWSVMIAQNLADATARAESRGETAVMVAADGEALGVICIADTVK
                     PTSAAAVRRLRDLGLTPVLLTGDNEGAARAAAAQVGITDVRAGVSPAGKLDTITQLQA
                     AGHVVAMVGDGVNDSAALAAADLGIAMGGGTDAAIAASDITVVSGDLLVVGDAIRLAR
                     ATLRTIKGNLFWAFAYNIAAIPIAMLGLLNPLIAGAAMALSSVFVVTNSLRLRRFRAR
                     ETP"
     gene            complement(6577..6903)
                     /locus_tag="PLW15_06265"
     CDS             complement(6577..6903)
                     /locus_tag="PLW15_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOT34035.1"
                     /translation="MCTTDSLHNDLGLTDKNHACACGHGATTTDLAVDSAAIHEDYLV
                     TGMTCSHCVSSVTEEVSAIDGVDAVNISLNVGGASRVTVVSTKPVSPDAVRAAVTEAG
                     YALVAD"
BASE COUNT         1292 a         2505 c         2517 g         1202 t
ORIGIN      
        1 gccatcgtcg ccatcatcgc gctggtcgtc gtcaactcca tcaagccggc cggacccggg
       61 ccgcgcaaca tggccagcga cggaatccag ctgagccagg gcgccatcgc caccgagacg
      121 ccggggctga agcccggcga ggacccggtg ccaaacgtgc gggacgaggc atccggtgtt
      181 ctcgacatcc agatgtacgt ggactacctg tgccccatct gcggtcagtt cgaggcgacc
      241 aacggtgagt acatctcgag cctgctcgag aacggcggca ccacggtgga gatccacccc
      301 atcgcgattc tcgaccgact gtctcagggc accaagtact ccactcgcgc ggtgaatgcc
      361 gccgcgtgcg tggccgacat gtcgcccaac cagttctacg acttccacaa ccagctattc
      421 gccaaccagc ccgccgagaa tacgaccggt ctgaccgatg aggagatcat cgctctgacc
      481 gtggcggcca aggtggacaa cgctgacaag atcgcgagct gcatcaagga ccagtccttc
      541 aaggcttggg cgaccgctgc gaccgcacgt gcgctgaatg ggccgatccc gaactcgaac
      601 gtcgacaagg tcaccggcac gccgaccgtc atcgtgaacg gcctcaagta cgagggagcg
      661 gtcaacgacc tcgcctcgtt ccaggctttc gtgctgcagg cggccggctc ggacttcaac
      721 aacagctcga cctcgacccc gactccgact ccgtcgccca ccccggcggg ctaagacacc
      781 acacgacgaa gggggcggcc tgcgggtcgc cccctttgtg gtttctgcgg gtactcagtg
      841 atcgtggcgg tgatgcgcgt cggggtagtg ctcgtgtgtg tgggtcagtg catcgtgggt
      901 gtgcacatgc cggtgacgga tgcccggctc aaccgccgcg tcgtgctcgt gctggtggtg
      961 ctcatcgtgc gcgtgccagt ggtcgtgcgt gacggcgagg tgggtgtgct cgtgcacgtg
     1021 ccgctccgtg aggtgcagcc atactcccag ggccatgagg gcggcagcga tgatcagcgg
     1081 cacggtcact gacgcaccga gcgcaatggc gagggccgca ccgaagaatg gcgccaccga
     1141 gaagtaggct ccggcgcgag cggcgcccaa gtgtcgcata gcgacgatga agagcacgag
     1201 gctgactccg tatgctccga gcccgacgag catcgctgcc ccgacgctcg cggccggcgg
     1261 cagctgggct ccgagcgcga aggcgatgac gaggttgacg ggtcccgcca ccccgccctt
     1321 caccgcggcc agccaggtgg catcggtcag ggcaatcttg cgggtgaggt tgttgtcgat
     1381 tccccagcac aggcacgcgc ccagaatcgc gagcgagggc agcaccggtc cgagatccgc
     1441 tccggtcggg atgctcagca ccaccgctcc ggcgacgatg gcgagcatcc cgagggcgat
     1501 gcgccggtcg acattctcgc ggaagacgac ccacgcgatc acggccgtga agaggccctc
     1561 ggcattgagc agcagggatg ccccggtcgc aggcatcccg ctgagtccga acatgagcag
     1621 cacggggccg aggatcccgc cgaagccgat ggcgccgacg agaggcagga cttcggcgcg
     1681 cttcagccgc acccgttccg gccgggtcac gaatcgatac aggccgagcc cgatgcccga
     1741 gccgacgtag aggagccctg cgagcatcca cgggctcacc gagccgagaa gcagcttggc
     1801 caacggagtg ccagcgccga acaacactgc cgacgcgatg cccgcctgca ccccgggatt
     1861 gcggagactc tgaagaagcg gcacggtcta ttgcaccatg ccccctgccc ggtacgcccc
     1921 tacagagcgc gcaccgagtt gttgcgcgcg tcgtgagtga gttcgacgag cccgagcttc
     1981 tcgagcagcg ggggcaggta catcccgaac cgaccgcggt aacccttgcg caggccgtac
     2041 cacccgccga ccgggttcga cgcgtcgcgg ccccacgcct cgacggtgcc gtcggccgcc
     2101 gccttgctct cgtcggccgc gccgagctga acccagtcgc cctgctcctt gagccacgcg
     2161 gtcagatcct cgatcgcgcg ggcctggtac ttgagtgtcg tcgagccgac ctggcagacc
     2221 agttcatcgc cgtcgatgta catcgtgtag ttggagctac ctggggcggt gaccagctcc
     2281 caggggtcgt ccttggttcc tgtgtcagtc attgcttcct ctcgctgcca cgacgtgcgg
     2341 cagtaggcca gcccagcgcc gcgattgcgg tcgcgcccat tctgctcctg tgctcagcaa
     2401 ctggtccatc gcctgtgaac agaggtgatc ggagtaggga tgctcgacgc ccggctgtgg
     2461 cggccgacgg tgggcgtgga taccctgtcg ccatggcgat cgatcccgca ctgctgaact
     2521 ggctgctcga ctccgacccg gcgctgcgct ggcgcgttga gcgcgacctg gcgggcgctc
     2581 cacgcgagac ctgggcggcc acccgcgagt cgattgccac cgagggcttc ggtgcgagac
     2641 tgctcgagct tcaggatgcc gacggccagt gggcgggcgg atcgttcttc ccgaaggact
     2701 tcgacttcaa cggtcccgag gccgccgagg gtgccgggca gccgtggacg gcgaccacgt
     2761 ggacactgaa cacgctgcgc gagtggggac tcgacgcatc cgcactcgag ggcacggcag
     2821 agctgctcga cgccaacagt cgctgggact acaacgacct gccctactgg ggtggtgagg
     2881 tcgactgctg catcaactcg tacacgctcg ccaacggcgc ctggctcggc gccgacgtcg
     2941 caggcatcgc gcagtggttc cttgaccacc gcatggatga cggcggctgg aactgcgagt
     3001 gggagaacgg ctcgacccgc tcgtcgttcc actccacgct caacaccctc atcgcgctgc
     3061 tctactacga agaggtcacc ggcggcagcg acgcgctgcg tgaggcccgt cgcgcgggcg
     3121 aggaatacct gctgcagcgc cggctgatgc gcaggctcac gacgggcgag atcgtcgggc
     3181 catgggtgac gcggttcgcg tacccgttcc gccactacta cggggtgctg cgggcggtcg
     3241 actacttccg ctcggccacc ctgttcgacg gcaccgagcc cgacgagcgc atcaccgacg
     3301 ccgtcgaggc gatccgcgcc gcgcgccggc ccgacggcac ctggcttcag gagcgacgcc
     3361 acccgggtcg ggtctggttc gaggtggatg cgcctgtcgg cgagccctcg aagtggctca
     3421 cctactacgg cacgcgttca ctcgactggt gggacaacgg ctcggtcagc gtcatcccgc
     3481 acgtgtagcg gcccggctca ggcaccggcc gccgactgag cgtgccgttc cgcccgctcg
     3541 ccggcctctc ggcgcagcgc ggtggtcttg ccgatgtgcg ggttccatac gatgagtttg
     3601 cgcttgcggc cgctcagctc ctggtactgc gtgatgaggc tcatccgcgg aaacgtgttg
     3661 accagcacca cgtcgggcac gagccggtcg acgagtcggc cccacgcggg gctggggagc
     3721 gtggcatcct gcccggcctt cttcgcctcg aggtaggcgt tcttctgctc gacgcggcgg
     3781 taggccagca gggtgagcgt gatcgaggcc agcgtgagca ctatgagcac cacgagggcg
     3841 atgacgcccg ggatgaccgg gatgctgagg atcagcacga ggttgatcac gtcgagcacg
     3901 tagagcagga acaccgagag cagaccccag aagatcgcgt acttgaggca cacgcggccc
     3961 tggatgttga ggaactcctt gctgtagtcc cagtagacgg cgtggaagac cttctccatc
     4021 acgaggctcg tcacgtactc gagcacggtg ccgacgacca gaccgaggcc gaagatgatg
     4081 agcgggttgt cgaagtaggg caccagcacg agcgtgatga tcacgccgcc cgtgccgtag
     4141 agcaggttga acggcaggta cagcagcccc agccgggact cgatcacgcc cttgtactcg
     4201 atggtccagc agtagatcat ctcgacgatg acgccggcga agctgtagat gaagaacaac
     4261 cacagcgcca gatacgcctc ggcaaaaaac aggtccaacg acatcgatgc ctcctcgggg
     4321 cgcgctctcg ttggcagggt atagctacct acggtgtttc gcgcgcccgg aatcgccgca
     4381 ggcgcaggct gttcgtgacg acgaagaccg acgacagcgc catggcggcc ccggcgatca
     4441 atgggttgag aaggccgagc atcgcgatgg ggatcgcggc gatgttgtag gcgaatgccc
     4501 agaacagatt gcccttgatc gttcgcagag tcgccctggc caatcggatg gcatcgccca
     4561 ccacgagcag gtcaccgctg accacggtga tatcgctcgc agctattgcg gcgtcggtgc
     4621 cacctcccat ggcgatcccc aggtcggccg ccgcgagcgc ggccgagtcg ttgacgccgt
     4681 cgccgaccat cgccacgacg tgtccggccg cttggagttg tgtgatggtg tccagctttc
     4741 ccgcgggcga aactcctgcg cgcacgtcgg tgatcccgac ctgagcggca gcggcccgcg
     4801 cggctccctc gttgtcgccc gtgagaagta ccggagtcag cccgaggtcg cgcaggcgcc
     4861 tcacggccgc agcgctcgtg ggcttgaccg tgtcggcgat gcagatcacg cccagtgctt
     4921 cgccatccgc ggccaccatg accgcggtct cgccacggga ctctgctcgc gctgtcgcgt
     4981 cagcgagatt ctgggcaatc atgaccgacc agctctcggt gagccaggaa ctctttcccg
     5041 cgacaactgc gcggccgtcg acgatgccct gaactcccag tccggcatgg gcacggaatt
     5101 cagctgcggg cgagggtacc tcgggggcga aagccgcgat ggcacgagcg accggatgct
     5161 ccgagccctc ctcgaccgcg gctgcgagcc gcaccagctc ggcctccgtg accccggcag
     5221 caggcagcgc gtgcacgacg gacatgatgc cggtggtcac ggtgcccgtc ttgtcgagca
     5281 cgacggtgtc aacgcgtcgg gtcatctcga gcacctgcgg tccccggatg aggattccca
     5341 gttgcgatcc gcgacccgta cccacgagca gtgccgtcgg cgttgcgagc cccagcgcgc
     5401 acgggcaggc gatgatcagc gtggcgacgg cggccgtgaa tgcaatctcg ggggccgcgc
     5461 ccagggccag ccatgcgata agcgtgagag ccgcgagaac gagaacgacc ggcacgaaaa
     5521 ttccagcgac gcggtcggcg aggcgctgca cctgcgcttt gcccgtttgc gcgtcctcga
     5581 cgagtcgcgc aatgcgtgcg agctcggtgt cggcgccgac gcgcgtgatc tccacgagca
     5641 gccgtccacc aacgttcagt gtgcctccga ccactcggga gtcgatgccg acctcgacgg
     5701 gaatcgattc accggtgagc atgctggcat cgaccgccga agcaccgtcg agaacgagtc
     5761 catctgacgg gaacttctcg ccgggccgaa cgacgacctg atcgccagga accagcgttg
     5821 atgcgggaac gagcgtctcg acctcgccgc gacgcagggt ggcgttcttc gcccctactt
     5881 ccagcagtgc acgcagcgcc gcaccggagt cccgtttggc gcgcacctcg atgtatcggc
     5941 ccgcaagtat gaagacggtg actgcggatg ccacctcgag atagatctcg ctcgagcccg
     6001 tcgctggcga gccgaagaag gtgaagctca tgtgcatgcc cgcaaccccg gccgtgccga
     6061 agaacagcgc atagagcgac cagccgaatg cggcgagcac gccaacgctg atgagggtgt
     6121 ccatgctcgc ggcgccatgg cgcgcgttga tcgccgcgac tcgatggaac ggccatgcgc
     6181 cccagattgc gaccggtgag gcgagcgcca gggcgagcca ctgccagttg gtgaactgca
     6241 gtgcagggat catcgacagt gcggccactg gcgccgcgag cacggccgag acgatgaggc
     6301 gttgccgcag ggcgctcacc tcggtattgc ccgcagcctc agatgcctcc gcaacatcgg
     6361 ctacggggcg gggcacggac gccgagtagc cggcggattc gacggccgcg atgagggttg
     6421 cggcatccac accgtcggca gtgacccgcg ctttctcggt cgcatagttg acggtggcag
     6481 tcacaccgtc gattcggttg agcttgcgtt cgatgcgtgt ggcgcacgac gcgcaggtca
     6541 tccccccgat gtcgaggtca acgtcgctca cagtcgtcag tcagcgacga gcgcgtaacc
     6601 agcttcggtc actgccgcac gcacggcgtc ggggctcacg ggtttggtgc tgaccaccgt
     6661 cacccgggat gcgccgccca cgttgaggct gatgttcacg gcatcgacgc catcgatcgc
     6721 cgacacttct tcggtcacgc tgctgacgca gtggctgcac gtcattcccg tcaccagata
     6781 gtcctcgtgg attgcggccg agtcgaccgc gaggtcggtg gtcgtggctc catggccaca
     6841 ggcacaggcg tggttcttgt cggtcaggcc cagatcgttg tgaaggctgt cggtggtgca
     6901 catggtcgtt ctcttctttc gtgatgatcg gttcgtcgcc gttcgctatg tcaatggtac
     6961 cccctagggg tatatacacg gcgggacctt tggcccaccg cgcgcagggg ggagcgccca
     7021 tactgggcag aacacgcagc ggtcgacacc gtcgagggag acgtgtgatg cggggcgatg
     7081 cagtgaatcc ggggagggtc tctgatggag ccgatgagcc gacgtacggc actcacgttg
     7141 gggggaatcg gcgcagcggt cgtcgtcgcc ggcggtgcag gtctggtctg gatgcagacg
     7201 aaccagagtt cgccgaccac cactccgacg acacccggaa ccgggacccc tgccgcaccc
     7261 ggtgaccagc tcggcgagcc cgaggtgctt cgcagtgccg ggggagtctt ggatgtcacg
     7321 ctggtcgcgg ccgaatcggc ggtggcacct cggtgcgagc cctcaccttc aacggtggcg
     7381 tgcccggccc gacgctggtc gtcagaccgg gggatgtgct ctcggtcgcc ttccagaacc
     7441 aactggcgat gcccaccaac ctgcacacgc acggccttca cgtctcgccg gacggcatca
     7501 gcgacaacgt gttcat
//
LOCUS       DAOSOU010000089         7466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_9911_length_7466_cov_5.727162, whole genome shotgun sequence.
ACCESSION   DAOSOU010000089 DAOSOU010000000
VERSION     DAOSOU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9911_length_7466_cov_5.727162"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLW15_06270"
     CDS             complement(<1..221)
                     /locus_tag="PLW15_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT34036.1"
                     /translation="MSGHSKWATTKHQKAIKDQRRAKSFAKLIKNIEVAAKMGGADLS
                     GNPTLVDAIQKAKKTSVPNDNIDRAVKRGA"
     gene            263..655
                     /locus_tag="PLW15_06275"
     CDS             263..655
                     /locus_tag="PLW15_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34037.1"
                     /translation="MPAHWFRITYARNALLRSLAEAGLDLGALTPSSGLKAMLDFARD
                     FRPQHAELDELFCRWGPTEQRYEFAIVRRMRRHDHPEAQLSLVFEFAPTPSRVGSGTA
                     AVTTERDATSTDGYRAIVRAAVVGRRID"
     gene            complement(652..1257)
                     /locus_tag="PLW15_06280"
     CDS             complement(652..1257)
                     /locus_tag="PLW15_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34038.1"
                     /translation="MKLYSDFGPRRSRQIVADVTALALIGAWVWLGITVYSLIENLSV
                     YGVQMEDAGAGFRETMTQVGETLGGIPLIGGGIRTPFDGASEAGGALEAAGQSQQVAI
                     NQLATVLGIGIAALPILTILLLWLLPRLRFAQRASRAQKLVKSGAGVDLLALRALASQ
                     NISALAAVDPDAMAAWRRGDDQVMRKLAALELKSSGVRMAD"
     gene            complement(1285..1914)
                     /locus_tag="PLW15_06285"
     CDS             complement(1285..1914)
                     /locus_tag="PLW15_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOT34039.1"
                     /translation="MNMIHGMFRSILNEAPKLVRSVAQSDTEHAAAVAGHLSDLISGL
                     HNHHHGEDLLLWDELAARAPACAAHVDQMRAQHAAVSDDLDALEALIPAWRETADVAD
                     REAVAAAVETVRSALFAHLGQEEEQILPVASGVFSQPEWDKLGEHGRASVPRNRQLIQ
                     LGYILNSMSPEDARAWAKANLPVPIRLLYRLVGRRQFEAEQRAVRGAVV"
     gene            complement(2002..2595)
                     /gene="pdxT"
                     /locus_tag="PLW15_06290"
     CDS             complement(2002..2595)
                     /gene="pdxT"
                     /locus_tag="PLW15_06290"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOT34040.1"
                     /translation="MAGKPRVGVLALQGDFREHLAVLSDLGADAVPVKRPEELASVAG
                     LVIPGGESSVMDKLSRMFGLAEPLRAAVASGLPVYGTCAGLIMLSNTVLDSISGQESI
                     GGLDIVVRRNAFGSQLDSFETDLDIPVLGETPMHAVFIRAPVVESVAPGVTVLAALGD
                     GRVVAVEQGSLLGTSFHPEMTGDTRFHEYFLDKVRAA"
     gene            complement(2585..3472)
                     /gene="pdxS"
                     /locus_tag="PLW15_06295"
     CDS             complement(2585..3472)
                     /gene="pdxS"
                     /locus_tag="PLW15_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOT34041.1"
                     /translation="MTEELGTSRVKRGLAEMLKGGVIMDVVNADQAKIAEDAGAVAVM
                     ALERVPADIRSQGGVARMSDPDLIDGIISAVSIPVMAKARIGHFVEAQVLEALKVDYI
                     DESEVLSPADYVNHIDKWKFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGTGDVSE
                     ATKHIRTILGEIRALSSKSPDELYVAAKELQAPYDLVVEVAATGKLPVVLFTAGGVAT
                     PADAALMMQLGADGVFVGSGIFKSGNPAQRAAAIVKATTFFEDPQVIAEASRGLGEAM
                     VGINVSDLPAPHRLAERGW"
     gene            complement(3525..>3883)
                     /locus_tag="PLW15_06300"
     CDS             complement(3525..>3883)
                     /locus_tag="PLW15_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT34042.1"
                     /translation="AYVLLNLYPYNSGHLLVCPYRHVATYDEATAEEVAEIGSLTQTA
                     MRVLTETAQCHGFNIGMNQGRIAGAGVAEHLHQHIVPRWALDSNFFPIVAGTKAVPRL
                     LGEVREEIANAWPTSH"
     assembly_gap    3884..3983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3984..>5452)
                     /gene="thrS"
                     /locus_tag="PLW15_06305"
     CDS             complement(<3984..>5452)
                     /gene="thrS"
                     /locus_tag="PLW15_06305"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT34043.1"
                     /translation="ELTIYDNVDGKTGEVYWKDLCRGPHLPNTRMIGNGWALVRNAAA
                     YWRGSEKNKQLQRIYGTAWPTKDELRAYQERQEEALKRDHRKLGAELDLFSFPEEIGS
                     GLAVFHPKGGIIRAEIENYMRERLLANEYELVNTPHITKGHLYEISQHLHWYKDGMFP
                     PMHLDEERDADGHVTRQGQDYYLKPMNCPMHNLIYRARGRSYRELPLRLAEFGTVYRY
                     EKSGTLQGLTRVRGLTQDDAHIYVTPEQVKPELARQLEFVLETLRGYGLDDFYLELST
                     RDPDKSVGTDEQWAEATETLREVAIDSGLELVDDPGGAAFYGPKISVQARDAIGRTWQ
                     LSTVQLDFNQPSLFELEYTASDGTRQQPIMIHRALLGSIERFFAILLEHYAGAFPVWL
                     SPVQVVGIPVAEAHEEYLGGVIRQLKQAGVRAELDASSERMQKKIRTHTKDKVPFQLI
                     AGEEDSANGSVSFRFRDGTQENGVPIADAIARIRQAIDT"
     assembly_gap    5453..5552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5553..5854)
                     /gene="thrS"
                     /locus_tag="PLW15_06310"
     CDS             complement(<5553..5854)
                     /gene="thrS"
                     /locus_tag="PLW15_06310"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617471.1"
                     /note="catalyzes the formation of threonyl-tRNA(Thr) from
                     threonine and tRNA(Thr); catalyzes a two-step reaction,
                     first charging a threonine molecule by linking its
                     carboxyl group to the alpha-phosphate of ATP, followed by
                     transfer of the aminoacyl-adenylate to its tRNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT34044.1"
                     /translation="MRVNGELKDLAATAEDTDTVEPVTIDSADGLNILRHSAAHVMAQ
                     AVQSINPEAKLGIGPPVTDGFYYDFDVTEPFQPEDLKAIEKAMDRIIRQGQRFQRRV"
     gene            5970..6398
                     /locus_tag="PLW15_06315"
     CDS             5970..6398
                     /locus_tag="PLW15_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT34045.1"
                     /translation="MTEDVRREVAVAAGQHRAFELFTDHIGAWWPLEEHGVFPDSTVS
                     FEGDLLVERSGDQESVWGEVLAWDPDRAVTLTWHPGSAIEHATELNVTFVAVADETLV
                     TIIHTGWERMPDPDAAAAEYGSGWPGVLTRFRELVDEAGA"
     gene            complement(6403..6903)
                     /locus_tag="PLW15_06320"
     CDS             complement(6403..6903)
                     /locus_tag="PLW15_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34046.1"
                     /translation="MLIRSRLIIVSLSIAVALSGCAGPSGPDGGPTPSTSAAASEDTA
                     VLLDQFVAALCPAVTTDYAFNDAWLDETSSLEAIVTSAAAARDASATAVDAVEGLSTT
                     WPAEYGADLSLVSELYVTKGADYAAIAGAASLSELVDFEFADATEGNAAAQRIADALG
                     EPNITC"
     gene            complement(7019..7090)
                     /locus_tag="PLW15_06325"
     tRNA            complement(7019..7090)
                     /locus_tag="PLW15_06325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7056..7058),aa:Val,seq:gac)
     gene            complement(7112..7182)
                     /locus_tag="PLW15_06330"
     tRNA            complement(7112..7182)
                     /locus_tag="PLW15_06330"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7148..7150),aa:Cys,seq:gca)
     gene            complement(7238..7309)
                     /locus_tag="PLW15_06335"
     tRNA            complement(7238..7309)
                     /locus_tag="PLW15_06335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7275..7277),aa:Gly,seq:gcc)
BASE COUNT         1171 a         2384 c         2403 g         1308 t
ORIGIN      
        1 gcgccgcgct tgaccgcgcg gtcgatgttg tcgttgggga ccgaggtctt cttcgccttc
       61 tggatggcat cgaccagggt cgggttgccc gagaggtcgg cgccgcccat cttggctgcg
      121 acttcgatgt tcttgatgag tttggcgaac gacttggcac ggcgctgatc cttgatcgcc
      181 ttctggtgct tcgtggttgc ccatttggag tggcctgaca tgctgaacat tctagccagc
      241 ggcccccggg tagcattcga ccgtgcctgc tcactggttc cgcatcacct atgcacgcaa
      301 cgcgctgctg cgctcactgg ccgaggccgg gctcgatctc ggcgcgctca ccccgtcgag
      361 cggcctcaag gcgatgctcg acttcgctcg tgacttccgc ccccagcacg ccgagctcga
      421 cgagctgttc tgccggtggg gccccaccga gcagcggtac gagttcgcta tcgtgcgacg
      481 gatgcgccgc cacgaccacc ccgaggcgca gctgtcgctc gtgttcgagt tcgccccaac
      541 tccctctcgc gtcggcagcg gaacggcggc agtgaccact gagcgggacg cgacatccac
      601 cgacggctac cgcgcgattg tcagggcggc cgttgtaggt cgacgcatcg actagtcggc
      661 catccgtacg ccggaagact tcagctcgag tgccgcgagc ttgcgcatga cctggtcatc
      721 gccccggcgc caggcggcca tcgcgtcggg gtccacggca gcaagcgctg agatgttctg
      781 gctggcgagt gcgcgcagcg ccagcaggtc gactcccgcg cccgacttga cgagcttctg
      841 ggcacgggat gcccgttggg cgaaccgcag gcgcggcagc agccacagca gcaggatcgt
      901 gaggatcggc agcgcagcta tgccgatacc cagcaccgtg gcgagctggt tgatcgcgac
      961 ctgctggctc tgaccggccg cctcgagcgc tcccccagct tcgctcgccc cgtcgaacgg
     1021 cgtgcggatc ccgcccccga tgagcggaat gcctccgagc gtctccccca cctgggtcat
     1081 cgtctcgcgg aagccggcac cggcatcctc catctgcact ccgtagacgg agaggttctc
     1141 gatgagcgaa tagaccgtga tgccgagcca cacccaggcc ccgatgagcg cgagtgctgt
     1201 gacgtcagcg acgatctgac gagatctgcg gggcccgaag tccgaataca gtttcatggc
     1261 cccacgctag gccagcggga agtctcagac caccgcgccg cgcaccgcac gctgctcggc
     1321 ctcgaactgg cgtcggccga cgagtcggta cagcagtcga atcggcacgg gcagattcgc
     1381 cttcgcccac gcacgggcat cctcggggga catggagttg aggatgtacc cgagctggat
     1441 cagctggcga ttgcgcggca cggaagccct gccgtgctcg cccagcttgt cccattccgg
     1501 ttggctgaac acgccgctcg cgaccggcag gatctgctcc tcctcctggc ccagatgcgc
     1561 gaacagggcc gaacgcacgg tctccaccgc ggcggcaacc gcctcgcggt ctgcaacgtc
     1621 agccgtctcg cgccaggcgg gaatgagggc ttcgagcgca tccagatcgt cggagacggc
     1681 tgcgtgctgt gcccgcatct ggtcgacatg ggcggcgcag gcgggagccc gggccgccag
     1741 ttcgtcccac aggaggaggt cttcgccgtg gtgatggttg tggagcccgg aaatgaggtc
     1801 gctgaggtgg ccggccactg cggcggcgtg ctcggtgtct gactgcgcaa ccgaccgaac
     1861 gagcttcggc gcctcgttca ggatgctccg gaacatgcca tggatcatgt tcatgtccga
     1921 cgtgtcgcac ccgggcttgc cggtgctggg aagttgagtg accatcgatt cctcctccga
     1981 atgaggaatg ctagcagtgg ctcaggcggc ccgcaccttg tcgaggaagt actcgtgaaa
     2041 gcgcgtgtca cccgtcatct cggggtggaa cgacgtgccc agcaggctgc cctgttcgac
     2101 ggccacgacc ctaccgtccc ccagcgcggc gagtaccgtg actccggggg ccaccgactc
     2161 cacgacgggc gccctgatga acacggcatg catcggagtc tcgcccagta ccgggatgtc
     2221 gaggtccgtc tcgaaagagt cgagctggga cccgaacgcg ttgcggcgca cgacgatgtc
     2281 caagccgccg atactctcct gtccggagat cgagtcgagg acagtgttcg acagcatgat
     2341 cagccctgca cacgtgccat agacgggcag cccggatgcc acggcagccc gcaacggctc
     2401 ggcaagcccg aacatgcggc tcagcttgtc catcacgctg gactcacccc cggggatcac
     2461 gaggccagcg accgacgcga gctcctcggg tcgcttcacc ggcacggcgt cggccccgag
     2521 atcgctcagt accgcgaggt gctcccggaa gtcgccctgg agggcgagga caccgaccct
     2581 gggcttacca gccacgctcg gcgaggcggt gcggcgctgg gaggtcggag acgttgattc
     2641 cgaccatcgc ctcgccaagc ccgcgggagg cctccgcgat gacctgcggg tcctcgaaga
     2701 aggtcgtggc cttgacgatc gcggcggcgc gctgggccgg gttgcccgac ttgaagattc
     2761 ccgagccgac gaagacgccg tcagcaccga gctgcatcat gagcgcggca tccgccgggg
     2821 tcgcgacacc accagcggtg aacagcacca cggggagctt gcccgtcgcc gcgacctcga
     2881 cgacgaggtc gtagggcgcc tgcagctcct tggccgcaac atagagctcg tcggggctct
     2941 tggacgacag cgcacggatc tcgccgagaa tcgtgcggat gtgcttggtc gcctcagaga
     3001 cgtcgccggt accggcctca cccttcgacc ggatcatcgc tgcaccctcg gtgatgcgac
     3061 gcagcgcctc acccaggttc gtggcgccgc acacgaatgg cacggtgaac ttccacttgt
     3121 cgatgtggtt cacgtagtcg gcgggagaca gaacctcaga ctcgtcgatg tagtcgacct
     3181 tgagggcctc gagtacctgc gcctcgacga agtggccgat acgggccttg gccatcacgg
     3241 ggatcgacac ggcgctgatg atgccgtcga tgaggtcggg gtcgctcatg cgagccaccc
     3301 caccctgaga ccggatgtcg gccggtaccc gctcgagggc catcacggcc acggcgcccg
     3361 catcctcggc gatcttcgcc tggtcggcgt tgacgacgtc catgatgacg ccgcccttga
     3421 gcatctcggc gagtccacgc ttgacgcggc tggtgcccag ttcttctgtc atgatcctag
     3481 attctagttc ggtgtactgc cggtgatctc cggccaatcg attgtcagtg gcttgtcggc
     3541 cacgcgttcg cgatctcctc gcgaacctcc cccagaagcc gcggaaccgc tttggttcca
     3601 gcgacgatcg ggaagaagtt ggaatccagc gcccaccgcg gaacgatgtg ctgatgcagg
     3661 tgctccgcga cgcccgcccc cgcgatgcgc ccctggttca tgccgatgtt gaacccgtgg
     3721 cactgcgcag tctcggtcag cacgcgcatg gccgtctgag tcagactacc gatctcggcg
     3781 acctcctcgg cggtcgcttc gtcataggtc gcaacatgac gatacgggca caccagaagg
     3841 tgtccgctgt tgtagggata caggttgagc agcacgtagg cgtnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnttcgtgt cgatggcctg ccggatgcgc gcgatcgcat
     4021 cggcgatcgg cacgccgttc tcctgcgtgc cgtcgcggaa ccggaagctc accgagccgt
     4081 tggcgctgtc ctcttcgccc gcgatgagct ggaacggcac cttgtccttg gtgtgcgtac
     4141 gaatcttctt ctgcatgcgc tcactggagg catccagttc ggcgcgaaca ccagcctgct
     4201 tcagctgacg gatgacacca ccgagatact cctcatgcgc ttcagccacg gggatgccca
     4261 cgacctgcac tggtgacagc cagaccggga acgctcccgc gtagtgctcg agcaggatcg
     4321 cgaagaagcg ctcgatcgac ccgagcagcg ctcggtggat catgatcggc tgctggcggg
     4381 tgccgtcgga ggcggtgtac tcgagctcga acagcgaggg ctgattgaag tcgagctgca
     4441 ccgtcgagag ctgccaggtg cggccgatcg cgtcgcgagc ctgcaccgag atcttcggtc
     4501 cgtagaaggc cgctcctccc ggatcgtcga cgagttcgag acccgagtcg atggccacct
     4561 cgcgcagggt ctcggtggcc tcggcccact gctcgtccgt acccaccgat ttgtcggggt
     4621 cgcgcgtcga gagttccaag tagaagtcat cgagtccgta gccgcgcagg gtctcgagca
     4681 cgaactcgag ttggcgggcg agctccggct tgacctgctc gggcgtgacg tagatgtgcg
     4741 cgtcatcctg agtcaggcca cgcacgcggg tcagcccctg cagggttccg ctcttctcgt
     4801 agcggtagac ggttccgaat tcggcgagtc gaagcggaag ctcgcggtag ctgcgccctc
     4861 gcgcccggta gatcaggttg tgcatcgggc agttcatggg cttcaggtag tagtcctggc
     4921 cctggcgggt gacgtggccg tcggcgtccc gctcctcgtc aaggtgcatg ggcgggaaca
     4981 tgccgtcctt gtaccagtgc aggtgctggc tgatctcgta gaggtgaccc ttggtgatgt
     5041 gcggggtgtt caccagctcg tactcgttgg cgagcaggcg ctcgcgcatg tagttctcga
     5101 tctccgcgcg gatgatgccg cccttggggt ggaagaccgc gagtcccgag ccgatctcct
     5161 ccgggaacga gaacaggtcg agctcggcgc ccagcttgcg gtggtcgcgc ttgagcgcct
     5221 cttcctgtcg ctcctggtac gcccgcagct cgtccttggt gggccaggcc gtgccgtaga
     5281 tgcgctgcag ctgcttgttc ttctccgagc cgcgccagta ggcagccgcg tttcgcacca
     5341 gcgcccaccc gttgccgatc atgcgggtgt tgggcaggtg cgggccgcgg cagagatcct
     5401 tccagtagac ctcgccggtc ttgccgtcga cgttgtcgta gatcgtgagc tcnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacccgacg ctggaatcgc tggccctggc
     5581 ggatgatgcg gtccatggcc ttctcgatgg ccttgagatc ctcgggctgg aacggctccg
     5641 tcacatcgaa gtcgtagtag aagccgtcgg tcaccggcgg accgatcccg agcttcgcct
     5701 ccgggttgat cgactgcacg gcctgtgcca tcacgtgggc cgcggagtgg cggaggatgt
     5761 tcagtccgtc tgcggagtcg atcgtcaccg gctcaaccgt gtcggtgtcc tccgcggtgg
     5821 ccgcgaggtc cttgagctcg ccgttgacgc gcatggcgac gacggcacgg tcggtgaaga
     5881 gttcgaatcc ggtgttactc acccctcaag tttaggggcg gcaggacttg cccctcgccg
     5941 gtggcgacga ggcgcgtacc cttgcgggca tgactgaaga cgtgcgtcga gaggtagccg
     6001 ttgcggcggg ccagcaccgt gcattcgaac tgttcaccga tcacatcggc gcctggtggc
     6061 ctctcgagga gcacggagtg ttcccggact cgacggtgtc attcgagggc gacctgctcg
     6121 tcgagcgctc gggcgatcag gagtcggtgt ggggcgaggt tctcgcgtgg gatccggatc
     6181 gcgcggtcac cctcacgtgg catcccggat ctgccatcga gcacgcgaca gagctgaacg
     6241 ttaccttcgt tgccgtggct gacgagaccc tggtcacgat catccacacc gggtgggagc
     6301 gcatgcccga cccggatgcc gcggccgccg aatacgggtc cggatggccg ggcgtgctca
     6361 cgcgcttccg cgaactggtc gatgaggcgg gggcatgaac cctcagcagg tgatgttcgg
     6421 ctcccccaag gcgtcggcga tccgctgggc cgcagcattg ccctcggtcg cgtccgcgaa
     6481 ttcgaagtcg acgagttcgc tcagggacgc cgcgccggcg atggcggcgt agtcggcccc
     6541 cttcgtcacg tacaactcac tgacgaggga aaggtcggca ccgtactccg ctggccaggt
     6601 cgtcgacagc ccttccacgg cgtccaccgc ggtggcgctg gcgtctcgtg ctgcggccgc
     6661 tgacgtcacg atcgcctcaa gcgacgacgt ctcgtcgagc catgcatcgt tgaacgcgta
     6721 gtcggtagtt acggccgggc acagcgccgc aacgaactga tcgagaagta ccgccgtgtc
     6781 ctcggatgcc gcggcacttg tcgacggggt cggcccgccg tcgggcccgc tcgggccggc
     6841 gcatccggag agcgcaaccg caatggacag cgaaacaatg atcaggcgcg atcggatcaa
     6901 cacggcaata cctcccgggt gtcaggaaag ggctggccaa tcttctcaga tcggcgcggt
     6961 cctgtagccg ccagtcgact taaacaagaa ctcccccgat ttctcggggg agtcagtgtg
     7021 ggcggtactg ggatcgaacc agcgacctct tccgtgtcag ggaagcgcgc taccgctgcg
     7081 ccaaccgccc atggtttcgg atatgcatcc gaggtggcga cgggatttga acccgtgtac
     7141 acggctttgc aggccgttgc ctcgcctctc ggccacgcca ccgcgtgcgg gccgtgacag
     7201 agacctagaa ccgatggtca ctgacacttc ccactcgagc ggatgacgag attcgaactc
     7261 gcgaccctca ccttggcaag gtgatgcgct accactgcgc cacatccgca ttcgctcgca
     7321 ttgctgcgtg ctgtacgact gtaaccgatc agactgccga gggccaatcc tgttacgggc
     7381 gggtcgcggg ctgtggcaag attgtggtcg cgggcgattg gcgcagttgg tagcgcgctt
     7441 ccttcacacg gaagaggtca tcggtt
//
LOCUS       DAOSOU010000090         7313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10193_length_7313_cov_5.483191, whole genome shotgun sequence.
ACCESSION   DAOSOU010000090 DAOSOU010000000
VERSION     DAOSOU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10193_length_7313_cov_5.483191"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLW15_06340"
     CDS             complement(<1..1125)
                     /locus_tag="PLW15_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT34047.1"
                     /translation="MRSLRARATLVTIAVAVIAVIVTGLISLQLVRSSVNQDARDQLS
                     AQADILARMPRLATAGELAEKASLALGGTQVALVQPDGTAEGDAADYIDAAILRKLAA
                     GESVSTVRRGDPGMVLIEARPVAGGGAVVLALPLASIDRALGQATGRILVALGIGLAV
                     AIIGGWLLASWLSRPLTNAAAAARRLAAGERGVRMPDSPTSEMADVTDALGALDSALA
                     ASESRQREFLLSISHELRTPLTAVRGYAEAMADGLIGPDDVTSVGGTLVAETERLDQF
                     VGDLLELARLEADDFSITVSTVELEPLLVEVAAAWAGRAAVLGVGISAGSITGGGGTV
                     ETDPRRLRQVIDGLVENALRAGGSVQLRGSDGVVEVLDDGP"
     gene            complement(1122..1796)
                     /locus_tag="PLW15_06345"
     CDS             complement(1122..1796)
                     /locus_tag="PLW15_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT34048.1"
                     /translation="MSEGRGLVVIVEDEPAIADVERLYLTQAGFGVHIERNGLAGLAA
                     IRTLRPVAVVLDVGLPGMDGIELCRTLRAEDDWTPLIFVTARDDEVDRVLGLELGGDD
                     YLTKPFSPRELVARLRGLLRRQDGPGTPTPVTVGEVTLDSARRRVTSGGEEIALTTTE
                     FELLAKLMSSPGQVFSREQLLSSVWGHVDYGGGRTVDVHVAQLRAKLGESSPIRTVRG
                     IGYSAS"
     gene            1947..2453
                     /locus_tag="PLW15_06350"
     CDS             1947..2453
                     /locus_tag="PLW15_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34049.1"
                     /translation="MTIRTWAAAAASTAILATALVVAPSAAFAADGAAAASADCNFGQ
                     RVMNVWRQLPDELQADLKHLRELEPSARRNAAIEIRQKALAGDYGDETQARAEMMKDR
                     RMLAIETMPIELKHALLDLRNADPGDRRELAQQIADDALAGVYGPQAQHTVETIRASD
                     FWQECIAD"
     gene            complement(2450..3511)
                     /locus_tag="PLW15_06355"
     CDS             complement(2450..3511)
                     /locus_tag="PLW15_06355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT34050.1"
                     /translation="MTATIDELPIPESMDAPGGDDFARAVEVGNVVEAVTYGTPDLAY
                     EPAEELPYYRDPFHPQRMLVARLDGEIVGTARYETQSEDDADTAWIGVYVLPEFQRQG
                     IGRALAEAVEELSRAEGRTKVVVYHGVQAGAGEQLASPTGFGSVPADDRAVRLLLARG
                     YRLEQVERVSRLALPVEALEDRLAAAQLASGPDYALHYWYGPTPERWRADVAVLGNRM
                     STDAPSAGLEEPEETWTEERVIAADVRDAASPRTRTVAAVEHVPSGHLVGFTVLSVPQ
                     QAHRSAAQYATLVLREHRGHRLGMLLKVANLAHLERVSPGHPSVTTFNAEENRHMLSV
                     NEALGFVPIAYEGAWRRDL"
     gene            3647..>4569
                     /locus_tag="PLW15_06360"
     CDS             3647..>4569
                     /locus_tag="PLW15_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34051.1"
                     /translation="MYLKSLTLKGFKSFAQPTTFQFEQGVTCVVGPNGSGKSNVVDAL
                     AWVMGEQGAKTLRGGKMEDVIFAGTATKGPLGRAEVILTIDNADGALPIEYSEVTISR
                     TLFRNGGSEYAINGEQCRLLDVQELLSDSGLGREMHVIVGQGQLDAVLHASPEDRRGF
                     IEEAAGILKHRRRKEKTIRKLEAMQANLTRLSDLAGEIRRQLKPLGQQAEIAKEAASI
                     AAIVRDARARLLADEVVELRAAITDFSRSESERKTERIVLQEQLDQNKLREGRLEQAQ
                     VGDAVDVARRVAFGLESAQERLRGLYTLANQR"
     assembly_gap    4570..4669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4670..7090
                     /locus_tag="PLW15_06365"
     CDS             <4670..7090
                     /locus_tag="PLW15_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34052.1"
                     /translation="LAGLASRVDVARSTLAAARGELLRQQNALESAEQRRDSTRTEFV
                     ALEAEAATSTEGDGTLDTAYEAAEALVASVQAEIEALRDDLHAAERERDALAARSSAL
                     SLAVDQKDGSSALVGAKLGGIRGLVAEHVKIEPGFEAAIAAALGSLADAVLADSQGAA
                     FDALAHSAAQDFGRVEVVVADSAAPKQAAAPAGVRAATEVVTAPDGVLGLLAGVYIAD
                     DLDAARSAFAKIGDATFVTKQGDVLTRYVLRGGSGAKRSRLELVAERDTAAAKLATVT
                     TQIERTRFALAEKRGALETAKTDAAAALTALREYDAALAAQSEQLGRFRIQLEAAQAE
                     WERLSQGLELTAEKVREAEAAVERAVADHQEYESAPRPMLDVSARDAVLAEVETARAA
                     ELEQRIQLETIRERVRAERARAEALAAQLVAEREAAEEQARLTVIRQRQVAAATEVIA
                     ALPAVLDSVDRSVSQARVDLTAKEAERSAQNEELATLRREESSLRERLSAISENVHGL
                     EMQIYEKKLHLSTLLERAGEELGLVEDVLVAEYGPQVPVPDEGGLSSAQARIETEDDD
                     DQEGFDTPPAEATQPAGKPFVRVEQQARLAAAERKLAQLGRVNPLALEEFEALEQRHK
                     FLTEQLTDLTNTRKDLLTIIDEIDEKMQDIFGSAFEDTRAAFNQVFPILFPGGTGSIH
                     LTDPDNMLTTGIEVTVKPAGKKIERLSLLSGGERSLAAVALLIAIFKARPSPFYIMDE
                     VEAALDDANLGRLLTIFEDLRETSQLIVITHQKRTMEIADALYGVSMRADGISAVVGQ
                     RVGERSAS"
BASE COUNT         1157 a         2589 c         2484 g          983 t
ORIGIN      
        1 cggaccgtcg tcgagtacct cgacgacgcc gtcggatccg cgcaactgca ccgacccgcc
       61 cgcccgcagc gcgttctcca cgagtccgtc gatcacctgc cgcaaccgcc gcgggtcggt
      121 ctcgacggtg ccgccgcctc cggtgatcga gcctgccgag atccccaccc ccaacacagc
      181 cgcgcgcccc gcccacgcgg cagcgacctc cacgagcagc ggctcaagct cgaccgtcga
      241 cacggtgatg ctgaagtcgt cggcctcgag gcgcgcgagc tcgagcaggt cacccacgaa
      301 ctggtcgagc cgttcggtct cggccacgag ggtgccgccc accgatgtca cgtcgtcggg
      361 cccgatgagg ccgtcggcca tggcctcggc gtagccgcgc accgcggtca gcggcgtgcg
      421 cagctcgtgg ctgatcgaca gcagaaactc gcgctggcgg gactccgacg ccgcgagtgc
      481 cgagtccagg gcacccaggg cgtcggtgac gtcggccatt tcgctggtgg gggagtcggg
      541 catccgtacg ccgcgttcgc ccgcagccag tcgccgggcc gccgcggcgg cattcgtgag
      601 tggacgggag agccagctgg ccagcagcca gcccccgatg atcgccacgg caagaccgat
      661 gccgagtgcc acgaggatgc gcccggtcgc ctgccccagc gcccgatcga tgctcgccag
      721 gggcagggcg agcacgaccg ccccacctcc cgcaactggt cgcgcctcga tcagcaccat
      781 gccggggtca ccccgtcgca ccgtggacac cgactccccg gcggcgagct tgcgcaggat
      841 cgcggcgtcg atgtagtccg cggcgtcgcc ctcggcggtg ccgtccggct gcaccagtgc
      901 gacctgcgtg ccgccgaggg cgaggcttgc cttctcggcg agctcgccgg cggtggcgag
      961 ccgcggcatc cgcgcgagga tgtcggcctg agcggagagc tggtcgcggg catcctgatt
     1021 gaccgatgag cgcacgagct ggagcgagat caggcccgtg acgatgacag cgatcacggc
     1081 gaccgcgatc gtgacgagag tcgcacgggc gcgcagcgac ctcatgatgc ggagtatccg
     1141 atgccgcgca cggtgcggat ggggctcgat tctccgagct tggccctgag ttgggcaacg
     1201 tgcacgtcga ccgtgcgacc gccaccgtag tcgacgtggc cccagacgct cgacagcagc
     1261 tgctcgcgcg agaacacctg cccgggcgat gacatcagct tggcgagcag ctcgaattcg
     1321 gtggtggtga gcgcgatctc ctcgccgccg ctcgtgaccc ggcggcgagc gctgtcgagg
     1381 gtcacctcgc cgaccgtcac gggagtagga gtgcccggtc cgtcctggcg gcgcagcagt
     1441 ccccgcagcc gcgcgaccag ttcgcggggc gagaagggct tggtgaggta gtcgtctccg
     1501 ccgagttcga gcccgagcac ccggtcgacc tcgtcatcac gagccgtcac gaagatcagg
     1561 ggagtccagt cgtcttcggc gcgcagcgtg cggcagagct cgattccgtc cattccgggc
     1621 aggccgacgt cgagcacgac ggcgacggga cgcagggtgc ggatggcggc gaggccggcg
     1681 agcccgttcc gctcgatgtg cacgccgaat ccggcctggg tgagatacaa ccgctccaca
     1741 tcggcgatgg caggctcgtc ctccacgatc accacgagcc cgcgcccctc cgacatggct
     1801 ccattcaagt gcatgggccc accagcgtgg aggctccgcc gtgggacttt acattccccg
     1861 aacattcgcc tgacacccgt cgtacagcca ccggcgagag tcggtgctgt cgccacctcc
     1921 ggcggccaga gacagggagt cacgacatga ccattcgcac ctgggcagca gccgctgcct
     1981 ccaccgccat cctcgccacc gccctcgtgg tggcgccgtc agccgccttc gccgcggatg
     2041 gtgcggccgc ggcatccgcc gactgcaact tcgggcagcg ggtgatgaac gtctggcgcc
     2101 agctgcccga cgagctgcag gccgacctca agcacctgcg cgagctggag ccgagcgccc
     2161 gtcgcaacgc cgcgatcgag atccggcaga aggcgctcgc cggcgactac ggcgacgaga
     2221 ctcaggccag ggccgagatg atgaaagacc gccgcatgct cgccatcgag accatgccca
     2281 tcgagctcaa gcacgcactt ctcgacctgc gcaacgccga tccgggcgac cgtcgcgagc
     2341 tggcgcagca gatcgcggat gacgcgctcg ccggtgtcta cggaccccag gcccagcaca
     2401 ccgtcgagac gatccgcgcg agcgacttct ggcaggagtg catcgccgac tagaggtcgc
     2461 gccgccacgc cccctcgtag gcgatcggca cgaagccgag ggcttcgttc acgctgagca
     2521 tgtgccggtt ctcctccgcg ttgaaggtcg tgaccgacgg atgcccgggc gaaacccgct
     2581 cgaggtgtgc gaggttcgcg accttcagca gcatgcccag ccggtgtccg cggtgttcac
     2641 gcagcacgag cgtcgcgtac tgggcggcgg accggtgagc ctgctgcggc accgacagca
     2701 ccgtgaagcc gacgaggtgt cccgacggaa cgtgctcgac ggcggcgacc gtgcgggtgc
     2761 gggggctcgc ggcatcccgc acgtcggcgg cgatcacgcg ctcctcggtc caggtctcct
     2821 cgggttcctc gaggccggcg ctcggggcgt cggtcgacat gcggttgccg agcacggcga
     2881 cgtctgcccg ccagcgctcg ggggtgggac cgtaccagta gtgcagggcg tagtccggcc
     2941 cgctggccag ttgggcggcg gcgaggcgat cctcgagcgc ttccaccggc agcgcgagcc
     3001 gactcacccg ctcgacctgc tcgagccggt aaccccgggc caggagcagt cgcaccgccc
     3061 ggtcatcagc gggcaccgag ccgaatccgg tcggcgaggc caactgctca cccgcgccgg
     3121 cctgcacccc gtggtagacg accaccttgg tgcgaccttc ggctcgcgag agctcctcga
     3181 ccgcctcggc cagagcccgc ccgattccct ggcgttggaa ttcgggcagc acgtagacgc
     3241 cgatccacgc cgtgtcggcg tcgtcctccg actgcgtctc gtaccgcgcg gtgccgacga
     3301 tctcgccgtc gaggcgggcc acgagcatcc gctgggggtg gaaggggtcg cggtagtagg
     3361 gcaactcctc ggcgggctcg taggccagat ccggggtgcc gtaggtgacg gcctcgacga
     3421 cattgccgac ctcgacggcg cgcgcgaagt cgtcgccccc gggtgcgtcc atcgactcgg
     3481 ggatcggcag ttcgtcgatc gtggcggtca cgacagcatc ctctcagtag gcagccgacc
     3541 agactaagcc tcacgatcgc ccggcgcgag cgatcgagac ggaagcgtta cctccgacac
     3601 gtcgggtacc gtagtcccag ctagcgtgac cgaccgaagg gctgccgtgt atctgaagag
     3661 cctgaccctc aaggggttca agtccttcgc acagccgacg accttccagt tcgagcaggg
     3721 cgtcacctgc gtcgtggggc ccaacgggtc gggcaagtcc aatgtcgtgg atgccctcgc
     3781 ttgggtcatg ggcgagcagg gagccaagac cctccgcggc ggcaagatgg aggacgtcat
     3841 cttcgcgggc acggcgacga agggtccgct gggtcgcgcg gaggtcatcc tgaccatcga
     3901 caacgcggat ggcgcgctgc cgatcgagta ctccgaggtc accatcagcc gcacgttgtt
     3961 ccgcaacggc ggcagcgagt acgcgatcaa cggtgagcag tgccgccttc tcgacgtgca
     4021 ggagctgctg agcgactccg gcctcggccg cgagatgcac gtcatcgtgg gccagggcca
     4081 gctcgacgcg gtgctgcacg cgagccccga ggaccgccgc ggcttcatcg aggaggccgc
     4141 gggcatcctc aagcatcgtc gccgcaagga gaagacgatc cgcaagctgg aggcgatgca
     4201 ggccaacctc acgcgcctga gcgacctcgc cggcgagatc cgtcgccagc tgaagcccct
     4261 gggccagcag gccgagatcg ccaaggaggc ggcctcgatc gccgccatcg ttcgggatgc
     4321 ccgtgcacgg cttctggccg acgaggtcgt cgagctccgc gccgccatca ccgacttcag
     4381 ccgcagcgag tcggagcgca agacggagcg catagtgctg caggagcagc tcgaccagaa
     4441 caagctgcgc gagggacgcc tcgagcaggc tcaggtcggc gacgccgtcg acgttgcccg
     4501 tcgcgtcgca ttcgggctgg agagcgcgca ggagcgactg cgcggcctct acacgctcgc
     4561 caaccagcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcgccggtct
     4681 tgccagccga gtggatgtcg cccgctcaac actcgccgca gcccgcggcg aactgctgcg
     4741 ccagcagaac gcgctcgagt cggccgagca gcgtcgggac tccacccgca ccgagttcgt
     4801 ggccctcgag gccgaggcgg cgacctccac cgagggtgac ggcaccctcg acaccgccta
     4861 cgaggccgcc gaggcactcg tggcgagcgt tcaggccgag atcgaggccc tgcgcgacga
     4921 cctgcacgcc gccgaacgcg agcgcgacgc cctcgccgcg cgcagctccg cgctgtcgct
     4981 cgcggtcgac cagaaggacg gctcgagcgc gctcgtggga gccaagctcg gcggcatccg
     5041 cggtctcgtc gccgagcacg tcaagatcga gcccgggttc gaggcggcca tcgccgccgc
     5101 gctcggatcg ctcgccgatg cggtgctcgc cgacagccag ggcgcggcat tcgacgctct
     5161 cgcgcactcc gccgcccagg acttcggccg cgtcgaggtg gtcgtcgccg acagcgcggc
     5221 accgaagcag gccgccgcgc ccgcgggcgt gcgcgccgcg accgaggtcg tcaccgcacc
     5281 cgacggtgtg ctgggcctgc tcgccggcgt ctacatcgcc gacgatctcg atgccgcgcg
     5341 ctccgccttc gccaagatcg gcgacgccac cttcgtcacc aagcagggtg acgtgctcac
     5401 ccgctacgta ctgcgcggcg gctccggcgc caagcgctcg cgcctcgagt tggtcgccga
     5461 gcgcgacact gccgccgcca agctcgccac tgtcaccacc cagatcgagc gcacccgctt
     5521 cgccctcgcc gagaagcgcg gagcgctcga gacagccaag acggatgccg ccgccgccct
     5581 caccgccctg cgcgagtacg acgcggccct ggccgcccag tccgagcaac tcggacgctt
     5641 ccgcatccag ctcgaggccg cgcaggccga gtgggagcgc ctctcgcagg gcctcgaact
     5701 caccgccgag aaggtgcgcg aagccgaggc cgccgtcgag cgtgccgtcg ccgaccatca
     5761 ggaatacgag tccgcgccgc gcccgatgct ggatgtctcc gcccgcgacg ccgtgctggc
     5821 cgaggtcgag acggctcgcg ccgccgaact cgagcagcgc atccagctgg agacgatccg
     5881 ggagagagtg cgcgcggagc gcgcacgcgc cgaggctttg gccgcacagc tcgtggccga
     5941 gcgggaggcg gccgaggaac aggctcggct caccgtcatc cgccagcgtc aggtcgccgc
     6001 ggcgaccgag gtgatcgcgg cgctgcccgc ggtgctcgac tcggtggacc gttcggtgtc
     6061 gcaggcgcgg gtcgacctga ctgcgaagga ggcggagcgt tccgcccaga acgaggagct
     6121 cgcgacactg cggcgcgagg agagttcgct gcgcgagcgg ctgagcgcga tcagcgagaa
     6181 cgtgcacggt ctcgagatgc agatctacga gaagaagctg cacctgtcga cgctcctgga
     6241 gcgggccggc gaggagctgg ggctcgtgga ggacgtgctc gtcgccgagt acgggccgca
     6301 ggtgcccgtg cccgacgagg gcgggttgag tagcgcgcaa gcgcgtatcg aaaccgaaga
     6361 cgatgacgat caggaaggtt tcgatacgcc tcctgcggag gctactcaac cagcgggtaa
     6421 gccgttcgtg cgcgtcgagc agcaggcgcg cctcgcggcg gccgagcgca agctcgcgca
     6481 gctcggccgc gtcaacccgc tggccctgga ggagttcgag gcgctcgagc agcgccacaa
     6541 gttcctcacc gagcagctga ccgatctgac gaacacccgc aaggacctgc tcacgatcat
     6601 cgacgagatc gacgagaaga tgcaggacat cttcgggtcc gcgttcgagg acacccgggc
     6661 ggccttcaac caggtcttcc cgatcctgtt cccgggcggc accggcagca tccacctcac
     6721 cgatcccgac aacatgctca ccacgggcat cgaggtcacg gtgaagccgg cgggcaagaa
     6781 gatcgagcgc ctgtcgctgc tctccggtgg ggaacgctcg ctcgccgcgg tggcgctgct
     6841 catcgcgatc ttcaaggcgc gccccagccc gttctacatc atggacgagg tcgaggccgc
     6901 cctggatgac gcgaacctcg ggcgactgct gaccatcttc gaagacctgc gcgagacatc
     6961 ccagctcatc gtcatcaccc accagaagcg caccatggag atcgccgacg ccctctacgg
     7021 cgtcagcatg cgcgccgacg gcatcagcgc cgtcgtgggg cagcgggtcg gggagcggtc
     7081 ggcgagctag acgcagccga gcgctcgctg cgactctcgc gcgggagata tcccagcgcg
     7141 ctggcacgaa tgggtgggca cgcggtaccg tgcagttgtg atgcgcctga gtccgggact
     7201 actgctcgtt gctgttctcg ccctgtcggg ctgcggcacc gagccagcac cgaccgcgcc
     7261 ccccacgtcg tcgacaccca ccgtcgtgcc cacgcggacc gcgtcaccga ctc
//
LOCUS       DAOSOU010000091         7298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10217_length_7298_cov_5.725804, whole genome shotgun sequence.
ACCESSION   DAOSOU010000091 DAOSOU010000000
VERSION     DAOSOU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10217_length_7298_cov_5.725804"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PLW15_06370"
     CDS             complement(<1..1105)
                     /locus_tag="PLW15_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOT34053.1"
                     /translation="MASVGTNWSGFYEYTASTVHRPRTVDEVQELVARTPRIRALGSR
                     HSFSGMADSAELVSLDALSPDIRIDADTETVSFNAGLRYGDLGTALQAQGWALRNLAS
                     LPHISVAGAIATGTHGSGDRNGTLSRAVAGLELVTASGELVTIGPIDEAFDGAVVSLG
                     ALGIVTRVTLDIEPTFDVRQDVYEGLTWTRLIENFDAIMGRAYSVSVFTDWSGESVGR
                     VWLKSVIGSARPPGELYGARPAAIEKHPLAEMEPTNTTQQSGVPGPWIDRLPHFRMEF
                     TPSNGNELQSEYIVPRRNAVAAIEAIRALNAQITPLLHVSELRTMAADSLWLSGAYET
                     DAVGIHFTWRFEPDAVLAVLPIVEQALAPFGARP"
     gene            1175..2554
                     /locus_tag="PLW15_06375"
     CDS             1175..2554
                     /locus_tag="PLW15_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34054.1"
                     /translation="MTRQQRLVLVIAVLASFVAFLDSSVINVALPAMTGELGGGLTVQ
                     QWIVDAYLITLGSLILLAGSLSDVYGRIVVLRVGLIGFGVASVLIAVAGSAEFVIAMR
                     AVQGIAGALLVPSSLAIIMSSFRDAAQAKAIGQWTAWTSAAFLAGPFLGGVFVDYLSW
                     RLVFAINVIPIIVTLILLGMLGQKDIRRAGVHIDYPGAVLGIIGLGGPVFALIEQGNL
                     GWAHPAIYLPFGLGLLSFAAFIWWQSRAKNPMLPLELFRIRNFSAGNLSTALVYGALS
                     IGGFIVVIFLQEVAGFTATAAALALLPVSIGNVLLSSFFGGLSGKYGPRLFMTAGPII
                     AGAGYLLLLGVGQSVNYFLNVLPGILLFAVGLSMTVAPLTAAVLGSVSAAQSGIGSAV
                     NNAVARVAGLVAIAMLGVIIVGKLDVDGFHRVLIVTAALLILGGLVSFVGIRNPRKVA
                     QPSGEQPAA"
     gene            complement(2531..3361)
                     /locus_tag="PLW15_06380"
     CDS             complement(2531..3361)
                     /locus_tag="PLW15_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT34055.1"
                     /translation="MSGTLPELLRATHPGPSVAVTAVGVVLGVGVGLEPGRLVLLGAA
                     LLAHQFSVGWSNDWLDADRDRAVGRTDKPLVSGALTVPMVRTAAFVALAFAVLITIAL
                     GWGATAAHVVFIACGWGYNLGLKSTPLSLGAYAVGFAALPLIVTLALPVPALAAPWAM
                     GAAALLGAAAHFANALPDLDDDEATHVRGLPQLIGARWSGALIGVALLAAAALVLFGS
                     GSVDAVRAIGAAIVAVVAVVCSVLAASSRARRLLFPLILIAAVILVALLAFSGGRLLA
                     "
     gene            3403..>3659
                     /locus_tag="PLW15_06385"
     CDS             3403..>3659
                     /locus_tag="PLW15_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34056.1"
                     /translation="MANAGVRGILGVTAIGLAVVLMAGCTAPPPVLRLASGAIARPVD
                     PIVLYADARLSVMTLDEKIESMLMVHVPGLDVGALEAFASE"
     assembly_gap    3660..3759
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3760..4745
                     /locus_tag="PLW15_06390"
     CDS             <3760..4745
                     /locus_tag="PLW15_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT34057.1"
                     /translation="VHVPGLDVGALEAFASENRIGGLILMGDNVPDPAADLASMTSGM
                     SAEPGLPLLLGIDQEGGIVRRIDTDTAASAEELRFLDPDASRDAFATRGALLQSLGVS
                     INFGIVADVTGDPDSFIYERSLGSTAADAAARVAAAVEGERGTVLSTLKHFPGHGVSP
                     GDSHSSIPTTQISLDEWRVDHEPPFAAGIDAGAEVVMFGHLQFDAVDPQPASLSPMWH
                     DLLRTELGFEGLIITDDMSMLQNSGRDDLVDPYQNAVRAVAAGNTMLLYVGPVDVPGV
                     VAEVRDAVQAGTIDEALIDDAAWRILAARRVESGQTGPFVHCFEQCQSIIE"
     gene            complement(4747..5826)
                     /locus_tag="PLW15_06395"
     CDS             complement(4747..5826)
                     /locus_tag="PLW15_06395"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HOT34058.1"
                     /translation="MSSRTLRELTALRRASQLLGERTDRTPADVVRWMLAMQGQDFPG
                     VKWSVGLRSPGSTDASIEAALAAGEIVRSWPMRGTLHLVAPEDLGWMLAIGRPRTATT
                     TATRRANLGITDRELRLVADTAREHLSGGRTSRRDDLLATFESVGVPTTGQRGYHLLL
                     HLALHGVLVFGPVHGKHQTFALLDEWVTAPRVLDGDEALAEFAGRYFRSHGPATERDF
                     AWWANITLGDARKGIALADLDSREFDSVPHYFSPGLEPAPEVAQVLAGFDEYLLGYQN
                     RSGVLAAEFSNRIVPGNNGIFLPTLILDGQVVGTWRRTQRATRVDIELLPFTPIAQRG
                     RASLERAFDRFADFIEKPVVLRWAP"
     gene            complement(5859..6173)
                     /locus_tag="PLW15_06400"
     CDS             complement(5859..6173)
                     /locus_tag="PLW15_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34059.1"
                     /translation="MNAVIATAPPVASGLPAAVTIAVAVTAVVALVMLAVMLRRTLRP
                     TKLTVGISVVTALGVLIGALLVGGSLTQPSTAAATENSPLRGVADAPIELKLTGLQLP
                     TI"
     gene            complement(6336..>7298)
                     /locus_tag="PLW15_06405"
     CDS             complement(6336..>7298)
                     /locus_tag="PLW15_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOT34060.1"
                     /translation="SLTLEVLLDHGFLAEADAWRSWLLRSIAGDPSDVQIMYGLAGER
                     RLDEYELATLPGYEGSSPVRVGNGAFLQYQGDVFGEVMVALQAARRLGAAEDDFSWPL
                     QRALMTYVEENWMRPDNGIWEIRGPQRHFTHSRVMVWAAFDCAIRAVREFGLEGPADR
                     WQEIRDEIRDEIEQRGWDAERGTYTQYFGSEGVDASLLQLPQVGYLAPDDPRMVSTVE
                     AIERELLHDGLLLRYRTDTGVDGLPAGEHPFLACSFWLVEQYASSGRMDDARVLMDRL
                     VSFANDVGLLSEEYDVAGDRQVGNVPQALSHLALVRAADAIARH"
BASE COUNT         1159 a         2373 c         2456 g         1210 t
ORIGIN      
        1 gcggtcgcgc accgaacgga gcgagggctt gctcgacgat cggcagcacg gccagcacag
       61 cgtcgggctc gaagcgccag gtgaagtgga tgccgacggc atccgtctcg taggcgccgc
      121 tcagccacag ggagtccgcg gccatcgtgc gcagctccga cacgtgcagt aatggggtga
      181 tctgcgcgtt cagcgcgcga atcgcctcga tcgcggcgac ggcattgcgt cgcggcacga
      241 tgtactcgct ctgcagctcg ttgccgttgc tcggcgtgaa ctccatgcgg aagtgcggca
      301 gcctatcgat ccacgggcct ggaactccgc tctgctgggt cgtgttcgtc ggctccatct
      361 ctgccagagg gtgcttctcg atcgcggcgg gtcgtgcacc gtagagttct ccgggtggcc
      421 gagcagatcc gatgacgctc ttcaaccaca cccgccccac tgactctccc gaccagtcgg
      481 tgaacacgct gacgctgtag gccctgccca tgatggcgtc gaagttctcg atcaatcgcg
      541 tccaggtgag gccctcgtag acatcctgtc gcacgtcaaa ggtcggctcg atgtcgaggg
      601 tcacgcgggt gacgatcccg agagcgccca gtgagacgac ggctccgtcg aacgcctcgt
      661 cgatcgggcc gatcgtcacg agttcgcccg atgcggtcac gagttcgagc ccggccaccg
      721 cgcgggacag cgtgccgttg cggtcgcccg agccgtgggt tcccgtggca atggcgccgg
      781 cgacggagat gtgcggaagc gatgcgagat tgcgcagcgc ccagccctgt gcctgcagcg
      841 cggtgccgag atcgccgtag cgaagaccgg cgttgaacga cacagtctcg gtgtcggcgt
      901 cgatgcggat gtcggggctc agggcgtcga gcgagaccag ttcggccgag tcggccattc
      961 ccgagaagga gtggcggctg cccagcgcgc gaatcctcgg cgtgcgggcg accaactcct
     1021 gcacctcgtc gacagtgcga gggcgatgca cggtgctcgc ggtgtactcg tagaagcctg
     1081 accagttggt gccgacggat gccatggcac cattctgtcc gcataaacct ccggggtcgg
     1141 catagatccc gcgctgcggg tgttgactga gtccatgacc cgccagcagc gcctcgttct
     1201 cgtcatcgcc gtgctcgcct cgttcgtcgc gtttctcgac agttcggtca tcaatgtcgc
     1261 cctgcctgcg atgacggggg agctgggtgg tgggctcacc gtgcagcagt ggatcgtgga
     1321 cgcctacctc atcacgctcg ggtcgctcat cctgctcgcc gggtcactgt cagacgtcta
     1381 cggccggatc gtcgtgctca gggtcgggct gatcgggttc ggggtggcat ccgttctgat
     1441 cgcggtcgcg ggctccgccg aattcgtgat cgccatgcgc gcagttcagg gcatcgctgg
     1501 cgcgctcctg gtgccgagct cgctggccat catcatgtcg tcgttccggg atgccgccca
     1561 ggccaaggcg atcggccagt ggacggcctg gaccagcgcc gcgttcctcg ccggcccgtt
     1621 cctcggtggc gtcttcgtcg attacctgag ctggcgcctc gtcttcgcca tcaatgtgat
     1681 cccgatcatc gtcacgctca tcctgctcgg gatgctcggc cagaaggaca ttcgcagggc
     1741 cggggtgcac atcgactacc ccggagccgt gctgggtatc atcggactcg gcggaccggt
     1801 gttcgccctg atcgagcagg gcaacctcgg ctgggcgcat ccagccatct acctgccgtt
     1861 cgggctcggg ttgctcagct tcgccgcgtt catctggtgg cagtcacgcg cgaagaaccc
     1921 catgcttccg ctggagctgt tccgcattcg caacttcagc gccggcaacc tttcgaccgc
     1981 gctggtctat ggcgcattgt cgattggtgg cttcatcgtg gtgatcttcc ttcaggaggt
     2041 cgccgggttc accgccaccg ccgcggccct ggccctgctc ccggtatcga tcggcaacgt
     2101 gttgctgtcg tcgttcttcg ggggcctcag cggaaagtac gggccgcgac tgttcatgac
     2161 cgcaggcccg atcatcgcgg gcgcaggcta cctcctgttg ctgggggtgg ggcagagcgt
     2221 caactacttc ctgaacgtgc tccccggcat cctgctcttc gccgtcggcc tgtcgatgac
     2281 cgtcgcacct ctgacggccg cggtactcgg atcggtctcg gccgcccagt cggggatcgg
     2341 ctcagcggtg aacaatgctg ttgcccgcgt cgccggactc gtggccatcg cgatgctggg
     2401 cgtgatcatc gtcggcaagc tcgacgtcga cggattccac cgcgtgctca tcgtgacagc
     2461 tgcgctgctg atcttgggcg ggctcgtgtc gttcgtgggc atccggaacc ccagaaaggt
     2521 cgcgcagcct tcaggcgagc agcctgccgc ctgagaaggc cagaagcgct accaggatca
     2581 ctgcggcgat caggatcagc ggaaacagca ggcggcgcgc tcgcgaggat gccgcgagca
     2641 ccgagcacac gaccgccacg acggcgacga tggccgctcc gatcgcccgc acggcgtcga
     2701 ccgatccgct tccgaagaga acgagggctg ccgcggccag gagcgccacc ccgatcagtg
     2761 cgcccgacca ccgtgccccg atgagctgag gaagacctcg cacgtgagtc gcctcgtcgt
     2821 cgtcgaggtc gggaagcgcg ttggcgaagt gcgcggcggc accgagcagc gctgcggcac
     2881 ccatcgccca cggcgcggcc agtgctggca cagggagagc gagcgtgacg atgagcggca
     2941 gggccgcgaa gccgactgcg tatgcgccga gtgacagcgg tgtcgacttc agccccaggt
     3001 tgtaccccca gccacacgcg atgaacacga catgggcggc ggtagcgccc cagcccagag
     3061 caatagtgat cagcaccgcg aaggcgagcg ccacgaacgc ggccgtgcgc accatcggca
     3121 ccgtgagggc acccgagacg agtggcttgt ccgtgcgacc caccgcgcga tcccggtcgg
     3181 cgtcgagcca gtcgttcgac cagccgacgg agaactggtg agccaggagc gccgcaccga
     3241 gcagcacgag tcgccctggc tcgaggccga ccccgacgcc gagaaccacg cccacagcag
     3301 tcaccgcaac gctaggaccg ggatgggtgg ctctgagcag ttcgggcaga gttccgctca
     3361 tggccccatc gtaggtggca cggccggtgt tagcatcgcg gcatggccaa cgcgggcgtt
     3421 cgaggcatcc tcggtgtcac ggcgatcggg ctggccgtgg tactgatggc cggctgcacg
     3481 gcaccgccgc cggtgcttcg actcgcgtct ggtgcaatcg cccgtccagt tgatccgatc
     3541 gtgctgtatg ccgatgctcg actgagcgtc atgacgctcg acgagaagat cgagtccatg
     3601 ctcatggtgc acgtgccagg actcgatgtg ggggcgctgg aggccttcgc gtcggagaan
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggtgcacgtg ccaggactcg
     3781 atgtgggggc gctggaggcc ttcgcgtcgg agaatcgcat cggcggtctc atcctcatgg
     3841 gtgacaacgt tcctgacccg gccgccgacc tcgcgtcgat gacgagcgga atgagcgcgg
     3901 aacccggcct gccgctgctg ctcggtatcg accaggaggg aggcatcgtc aggcgcatcg
     3961 acaccgacac cgccgcctcg gcggaggagt tgcgcttcct ggatcctgat gcctcgcgcg
     4021 acgcattcgc cacccgtggt gctctgcttc agagcctggg cgtgtcgatc aacttcggaa
     4081 tcgttgctga cgtcacgggg gatcccgact cgttcatcta cgaacggtcg ctgggctcca
     4141 ccgcagcgga tgccgcggct cgagtcgccg cggcggtgga gggtgagcgc ggcaccgtgc
     4201 tgtccacgct caagcacttc cccggtcacg gagtctcgcc gggcgactcg cactcgagca
     4261 tcccgacgac ccagatctcg ctcgacgagt ggcgagtcga tcacgagccg ccgttcgcgg
     4321 cgggaatcga tgccggcgcc gaggtcgtca tgttcggcca cctccagttc gacgcagtcg
     4381 acccgcagcc cgcgagcctg tcgccgatgt ggcacgacct gctgcgcacc gagttggggt
     4441 tcgaggggct catcatcacc gacgacatgt cgatgctgca gaattcgggt cgggacgacc
     4501 tcgtcgatcc ctatcagaac gcggtgcggg ccgtcgcggc gggcaacacg atgctgctgt
     4561 acgtgggtcc ggtggacgta ccgggagtcg ttgccgaggt tcgtgacgcg gtgcaggcgg
     4621 gcacgatcga cgaggccctg atcgatgacg cagcgtggcg catcctggcg gctcgccgtg
     4681 tcgagtcagg ccagacgggg ccattcgtgc actgcttcga gcagtgtcag tcgatcatcg
     4741 agtgacctac ggcgcccacc gaagaaccac gggcttttcg atgaagtcgg cgaaccgatc
     4801 gaaggcgcgc tcgaggctgg cccggccccg ctgcgcgatc ggggtgaagg gcagcagctc
     4861 gatgtcgact cgagtggccc gctgggtgcg acgccaggtt ccgaccacct gaccgtcgag
     4921 gatcagggtg ggcaggaaga tgccgttgtt tcccggcacg atgcggttgc tgaactcggc
     4981 cgcgagcact ccgctgcgat tctggtagcc gagcaggtac tcgtcgaagc cggccagtac
     5041 ctgcgcgacc tctggcgcgg gctcaagccc cggcgagaag taatgcggga ccgagtcgaa
     5101 ctcgcggctg tcgaggtcgg cgagggcgat gccctttcgc gcgtcgccga gggtgatatt
     5161 ggcccaccag gcgaagtcgc gctcggtcgc cggaccgtga ctacggaagt agcggcccgc
     5221 gaactcggcc agcgcctcgt cgccgtcgag tacccgtggg gcggtgaccc actcgtcgag
     5281 cagcgcgaag gtctggtgct tgccgtggac gggcccgaat acgagcacac cgtgcagcgc
     5341 gaggtgaagg agcaggtggt atccgcgctg accggtggtc ggcactccaa ccgactcgaa
     5401 ggtggcgagg aggtcgtcgc gccgcgaagt gcgtccgccc gacaggtgct ctcgggctgt
     5461 gtcagcgacg agtcgaagct cgcgatcggt gattcccaga ttcgctcgtc gagttgcggt
     5521 cgtggttgcg gtgcgcggtc ggccgatcgc gagcatccac ccgagatcct ccggggcaac
     5581 gaggtgcagc gtgccgcgca tcggccagga gcgcacgatc tcgccggccg cgagtgcggc
     5641 ctcgatcgag gcatccgtgc tgcccgggga gcgcaggccc accgaccact tcactcccgg
     5701 gaagtcctgg ccctgcatgg cgagcatcca gcgcaccaca tcggcgggcg tccgatccgt
     5761 ccgctcgccg agcagctgtg atgcgcgccg cagcgccgtc aattcacgga gcgtgcgcga
     5821 cgacattgcg ccagtatgcc gggacccggc gacaggcgtc agatggtggg aagctgcaga
     5881 ccggtcaact tgagttcaat cggcgcgtcc gcgacaccgc gcagcgggga gttctcggtt
     5941 gccgctgccg ttgatggctg ggtaagcgag ccaccaacga gcagggcacc gatgagaacc
     6001 ccgagggccg taaccacgct gatgcccacg gtgagcttgg tgggccgcag ggtgcggcga
     6061 agcatcacgg cgagcatcac gagtgcaacg accgccgtca cagcgacagc aatcgtcacc
     6121 gcggcgggca ggccagaagc gaccgggggg gcggtcgcga ttacagcgtt cattgtgctg
     6181 tgttcctttc gggtaggaac cggatctgtc gggtacagat cccagtcgcc ccgagtcctc
     6241 ctttaggggg actcgatggc cagcgttgaa aaagtatcag gggcggggtg gttgggtcaa
     6301 gcggcggcac gcccccgttt gggggtcacg gcacgtcaat gccgggcgat cgcgtcggcg
     6361 gctcgcacga gagcgaggtg cgagagggcc tggggcacgt tgccgacctg tcggtctccg
     6421 gctacgtcgt actcctcgct gagcagaccg acgtcgttgg cgaacgagac gaggcgatcc
     6481 atcagcaccc gcgcgtcgtc cattcgcccc gagctcgcgt actgttcgac caaccagaac
     6541 gaacaagcca gaaagggatg ctcgcctgcg ggaagtccgt cgacaccggt atcggtgcgg
     6601 tagcgcagca gaaggccatc gtgaagcagt tcgcgctcga tggcctcgac cgtcgacacc
     6661 atgcgcgggt cgtcgggcgc gagatatccc acctgcggta gctgaagaag tgaggcgtca
     6721 actccctcac tgccgaagta ctgagtgtag gtgcccctct ccgcgtccca gccccgctgc
     6781 tcgatctcgt cgcggatctc gtcccggatc tcctgccagc ggtcggccgg tccctccagg
     6841 ccgaactccc tcacggcgcg gatcgcgcag tcgaaggcgg cccacaccat caccctcgag
     6901 tgcgtgaaat gtcgctgcgg ccctcggatc tcccagatgc cgttgtcggg ccgcatccag
     6961 ttctcctcga cgtaggtcat cagcgcgcgc tgcagcggcc acgagaagtc atcctcggcc
     7021 gcccccagtc gacgggccgc ctggagggca accatcacct cgccgaacac gtcgccctgg
     7081 tactggagga acgccccgtt gccaactcgc actggcgacg atccctcgta tccggggagg
     7141 gtcgcgagct cgtactcatc gagcctgcgc tctcccgcca ggccgtacat gatctgaaca
     7201 tcagacgggt cgcccgcgat cgagcggagc agccacgacc gccacgcatc cgcctcggcg
     7261 aggaagccgt ggtcgagcag cacctccagc gtgaggga
//
LOCUS       DAOSOU010000092         7255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10306_length_7255_cov_7.080278, whole genome shotgun sequence.
ACCESSION   DAOSOU010000092 DAOSOU010000000
VERSION     DAOSOU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10306_length_7255_cov_7.080278"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLW15_06410"
     CDS             complement(<1..649)
                     /locus_tag="PLW15_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOT34061.1"
                     /translation="MNRLRIKHMTGFHYGGDVTASYNEARMLPVSSDGQLVLFSNLEI
                     LPISSHHNYVDYWGSRVSSFEILTPHKELALTATSLVEVRPRPHADDAIGWDDLAIAV
                     ERATETVEQLKQTRRTAPPAEVVDLAREIAAKHTNSCDAALEISTAIGDAVQYMQGVT
                     GVHSTAAEAWAVRKGVCQDIAHLALGALRSVGIPARYVSGYLHPKPSAGIGETVAG"
     gene            complement(646..1575)
                     /locus_tag="PLW15_06415"
     CDS             complement(646..1575)
                     /locus_tag="PLW15_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOT34062.1"
                     /translation="MLSRIAESLFWIGRYIERSDGTARILDVHLQLLLEDPWIEEDLA
                     CRSLLSVMGSEAPDDAVLTRQDILSILAVDRSEPASIAYSLGAARENARRAREIVSTE
                     LWECLNTTRARMPRKVSSDKVHEFFGWVRERSALAVGIIESATSRDEAWQFFTLGRSI
                     ERADMTARLLATRSLTEASGPSWTTILRSCGAYEAYLRTYRGVPSARNAAEFLLLDRL
                     FPRSILFSVTRAEQCMRDIEPRADRVGVSDQAQRLLGQIRSELEYRPISEILVDLTMH
                     MDNVQLATSAASEAIRQRYFPTNAAPSWVGETS"
     gene            complement(1569..>1858)
                     /locus_tag="PLW15_06420"
     CDS             complement(1569..>1858)
                     /locus_tag="PLW15_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34063.1"
                     /translation="TWVVGQDPLMVTRHSIQGLVAGQAAATEAISIITPEMLKASQQD
                     HAPHDRPLGHGQQEQQQQGRLSSAQSARIETAEMGFDTLQAATQPAGGAEC"
     assembly_gap    1859..1958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1959..3442)
                     /locus_tag="PLW15_06425"
     CDS             complement(<1959..3442)
                     /locus_tag="PLW15_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOT34064.1"
                     /translation="MADLFDGYGATATTRPGTPPWDEMFASPGTARASYKEIHATLAQ
                     MTQAELRGRTDALASSYLAQGVTFDFAGEERPFPLDAVPRVIDQAEWVEVEAGIKQRV
                     RALEAFLADIYGPQNAVKDGVIPARLVTSSAHFHREAAGIEPANGVRIQVSGIDLIRD
                     EQGGWRVLEDNVRVPSGVSYVISNRRVMAQTLPELFVSMRVRPVGDYPHKLLQALRAS
                     APGGVEDPTVVVLTPGVYNSAYFEHTLLARLMGVELVEGRDLFCSGGRVWMRTTAGPT
                     RVDVIYRRVDDEFLDPLQFRADSMLGSPGLLLAARLGNVTIANAVGNGVADDKLVYTY
                     LPELTRYYLGEDPILKNVDTWRLEDPGALEEVLDRLHELVVKPVDGSGGKGLVIGPAA
                     SADELAELKGRLLRDPRGWIAQPVVQLSTIPTLVDDGMRPRHADLRPFAVNDGNDVWV
                     LPGGLTRVALPEGELVVNSSQGGGSKDTWVVGQDPLMVTRHSIQG"
     gene            3501..4139
                     /locus_tag="PLW15_06430"
     CDS             3501..4139
                     /locus_tag="PLW15_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34065.1"
                     /translation="MTETSPIILVLPGGGYSRLAPHEGEPVAEWLRGIGWRARVVEYP
                     VQTRHPGPLDVVRAEIAAERAAGVGIVGVLGFSAGGHLAGHAALTLAGGERPDFAVLC
                     YPVVTMLADTHRGSRDQLLGPRPSRRLKRATSLERLVTPDAPPMFIWTTGEDKSVAIA
                     DHSYTLATALARNAVPHDFHVFERGAHGLGFAEGIPAEAWRGLAERWLEYRR"
     gene            complement(4136..4573)
                     /locus_tag="PLW15_06435"
     CDS             complement(4136..4573)
                     /locus_tag="PLW15_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOT34066.1"
                     /translation="MPLTGEYEPSTSDWARKQAELYEASGGTEGVDLRGHRVIVLTSV
                     GAKTGKLRKTALMRVEHDGVYAVVASLGGAPKHPVWYFNITANPHVELQDGPDKNDYL
                     AREVTGDEKAVWWERAVEAWPDYATYQEKTDRQIPVFVLEPMR"
     gene            4755..6395
                     /locus_tag="PLW15_06440"
     CDS             4755..6395
                     /locus_tag="PLW15_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOT34067.1"
                     /translation="MPSVSAVIATTLSAHVHDVFGVMGNGNAYFLDALETTSTVFTPV
                     RHEAAAVAAADAYFRACGRLAAATATYGAGFTNLITPLAEAVRARIPLVVIVGSAPES
                     GMRDWDVDQTALASAVGAATITVDRMDAQGQTERAVALARSTRMPVVLAVPYDIGRAS
                     VADPDAAPTSWVADDAPTAPLDADEIAAAAAALAGAARPLILAGRGAWLAQAGEVLGE
                     LADCVGALTATSALGAGLFGDSPWNLGIAGGFGSHESAALMHEADVVLVVGARLNQFT
                     MRFGSLLDPAATVIQIDTDPESRHPVTDLLLVGDAVDVGRALCDRLAEHRAITPWRAT
                     AGEVPHGLSSMVGEVAVLEDARLDPRNVFQRLDRALPLDRVVVQDGGHFIGWAPGYLR
                     IPAPNRLIMVGTALQTIGLGFASGVGAAVAAPDSTIVLATGDGGGLMALSDLQSFIAA
                     TRRGVVIVVNDAGYGAEMHQYGSKGLSATPMLIDDVDFAAVGTALGAHGLTVRTLDDL
                     DALERWLADGSDGVWVVDCKVSQSIIAPYMREIMVTGM"
     gene            complement(6400..>7255)
                     /locus_tag="PLW15_06445"
     CDS             complement(6400..>7255)
                     /locus_tag="PLW15_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT34068.1"
                     /translation="PEAGGPVVIGANWYRFRVDQRIRVARIESVCFLWVVQGSGSVTT
                     GGRTHSLSTSTVLRLPWGHDVDYEPHGTTPLHIGTVHLVPWHSLAVAVEPRVAFRADD
                     PLLGVPWRRGPAEPGIAAELPSRSVPGRSIIGLASYCVERFLTGRTEEPALRALGGLI
                     AEESGLEPARETEPTATPVALQAMTEHILSRLDQPLTVAEVARIGDCSTATAERLFSR
                     YTGHSVVSWTRHRRMQEAAILLRTTGLRVTEVARRVGFSDPAYFSRVFSAVHGTPPSR
                     YTEGQLRP"
BASE COUNT         1034 a         2458 c         2506 g         1157 t
ORIGIN      
        1 cgccagcgac ggtctccccg atgccagcgc tcggcttcgg gtgcaggtaa cccgagacgt
       61 accgcgccgg aatgcccacg gagcgcagcg cgccgagcgc gaggtgcgcg atgtcctggc
      121 agacgccctt gcgcacggcc cacgcctcgg cggcggtcga gtgaacgccg gtgaccccct
      181 gcatgtactg cacggcgtcg ccgatcgcgg tggagatctc cagagcggca tcgcaggagt
      241 tcgtgtgctt ggcggcgatc tcgcgggcca gatcgacgac ctcggccggc ggcgccgtgc
      301 gccgggtctg cttgagctgc tcgacggtct cggtggcgcg ttcgacggcg atcgccaggt
      361 catcccagcc gatcgcgtca tccgcgtggg gccggggacg aacctcgacg aggctcgtcg
      421 ccgtgagggc gagctccttg tgcggggtga ggatctcgaa cgacgagacg cgggatcccc
      481 agtagtccac gtagttgtgg tgactcgaga tcgggaggat ctcgaggttg gagaacagca
      541 ccagttgccc gtcggagctg accggcagca tccgggcctc gttgtaggac gccgtgacgt
      601 cgccgccgta gtggaagccc gtcatgtgct tgatgcgcag ccggttcacg aggtctcccc
      661 cacccagctc ggcgccgcat tggtcgggaa gtagcgctgg cggatcgctt cggatgcggc
      721 gctcgtcgcc agctgcacgt tgtccatgtg catggtgagg tcgacgagta tctcggagat
      781 ggggcggtac tcgagctcgc tgcggatctg gccgagcagt cgctgcgcct ggtcggagac
      841 gccgacccgg tcggcgcgcg gctcgatgtc gcgcatgcac tgctcggccc gcgtgaccga
      901 gaacaggatg ctgcgcggga acagccgatc gagcagcagg aactccgcgg cgttgcgcgc
      961 gctcggcacc ccacggtagg tgcgcagata cgcctcgtac gccccgcacg aacgaaggat
     1021 ggtcgtccag ctgggcccgg atgcctcggt caggctgcga gtggcgagca ggcgcgccgt
     1081 catgtcggcg cgctctatcg agcgacccaa cgtgaagaac tgccatgcct cgtcccggct
     1141 cgtggccgac tcgatgatgc ccacggccag cgccgagcgc tcgcgcaccc agccgaagaa
     1201 ctcgtgcacc ttgtcgctgg agaccttgcg cggcatgcgc gcgcgggtcg tgttgaggca
     1261 ctcccacagc tcggtggaga cgatctcgcg ggcgcggcgg gcgttctcgc gggccgcccc
     1321 cagggagtag gcgatcgacg ccggctccga gcggtcgacg gcgaggatcg agaggatgtc
     1381 ctgtcgggtg agcaccgcgt catccggcgc ctcactgccc atcacgctca gcagggagcg
     1441 gcaggcgagg tcctcctcga tccacgggtc ctccagcagc agctggaggt gcacatcgag
     1501 gatgcgcgcg gtgccgtcgg agcgctcgat gtagcgcccg atccagaaca gtgattcggc
     1561 gatgcggctc agcattcggc accgcccgct ggttgagtag ccgcttgcag cgtatcgaaa
     1621 cccatttcgg cggtttcgat acgcgcgctc tgcgcgctac tcaaccggcc ctgctgctgc
     1681 tgctcctgct gcccgtgacc gagcgggcga tcgtgcggcg cgtggtcctg ctgcgaggcc
     1741 ttgagcatct cgggggtgat gatcgagatg gcttcggtcg cggcggcctg gccggcgacg
     1801 agtccctgga tcgagtggcg cgtgaccatc agcggatcct ggccgacgac ccaggtgtnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ctggatcgag tggcgcgtga
     1981 ccatcagcgg atcctggccg acgacccagg tgtccttcga gccgccaccc tgcgacgagt
     2041 tgaccacgag ctcgccctcg ggcagcgcca ctcgcgtgag tccgccgggc agcacccaga
     2101 cgtcgttgcc gtcgttgacc gcgaagggcc tgaggtcggc gtgcctcggg cgcatcccgt
     2161 cgtcgacgag ggtggggatc gtcgacagct gcaccaccgg ctgggcgatc cagccgcgcg
     2221 ggtcgcgcag cagtctgccc ttgagctcgg cgagttcgtc ggcggatgcc gcggggccga
     2281 tcacgaggcc cttgccgccg gagccgtcca ctggcttgac cacgagctcg tgcaggcggt
     2341 cgagcacctc ctcgagggcg cccggatcct cgagccgcca ggtgtcgacg ttcttgagga
     2401 tcggatcctc accgaggtag tagcgggtga gctcgggcag gtaggtgtac acgagcttgt
     2461 cgtcggcgac gccgttgccg acggcgttgg cgatcgtgac gttgccgagg cgcgcggcga
     2521 gcagcaggcc ggggctgccg agcatcgagt cggcccggaa ctgcagcggg tctaggaact
     2581 cgtcgtcgac gcggcggtag atcacgtcga cgcgcgtggg gcccgcggtg gtgcgcatcc
     2641 agacccgacc accggagcag aacaggtcgc ggccctcgac gagctcgact cccatgaggc
     2701 gggcgagcag ggtgtgctcg aagtaggcgg aattgtagac accgggggtc agcacgacga
     2761 cggtcgggtc ctcgacgcca ccgggggcgg atgcccgaag cgcctgcagc agcttgtgcg
     2821 ggtagtcccc caccggccgc acccgcatcg acacgaacag ctcggggagg gtctgggcca
     2881 tgacgcggcg gttggagatc acgtagctga cgccgctggg cacccgcacg ttgtcctcga
     2941 gcacgcgcca gccgccctgc tcgtcacgga tcaggtcgat gccggagacc tggatgcgca
     3001 caccgttggc cggctcgatg ccggccgcct cgcggtggaa gtgagccgaa ctggtgacca
     3061 gccgcgccgg gatgacgccg tccttgaccg cgttctgcgg accgtagatg tcggcgagga
     3121 aggcctcgag ggcgcgcacg cgttgcttga tgcccgcctc gacctcgacc cactcggcct
     3181 ggtcgatcac gcgcggcacg gcgtcgagcg ggaacggccg ctcctcgccc gcgaagtcga
     3241 aggtgacacc ctgggccaga tacgagctgg cgagggcatc ggtgcggccg cgcagctcgg
     3301 cctgcgtcat ctgagcgagg gtcgcgtgaa tctccttgta ggaggcgcgg gcggtgcccg
     3361 gcgacgcgaa catctcgtcc cacggaggcg tgcctggtct cgtggtggcg gtggcgccat
     3421 agccatcgaa caggtcagcc atggcatgag cctacggctg tcgtgttgcg gtggtgtttc
     3481 accgcgttag ggtttcgtct gtgaccgaga cctcaccgat catcctcgtg ctgccgggcg
     3541 gcggctattc ccggctcgca ccccacgagg gcgagcccgt ggccgagtgg ctccgcggca
     3601 tcggctggcg tgcccgcgtc gtcgagtatc cggtgcagac gcggcatccg gggcccctcg
     3661 atgtggtgcg agccgagatc gccgcggagc gcgccgcggg cgtcgggatc gtcggagtgc
     3721 tcggcttctc cgcgggcggg cacctcgcgg gtcatgccgc tctcacgctc gcgggcggcg
     3781 agcgccccga cttcgccgtg ctctgctacc ccgtggtgac gatgctcgcc gacacccacc
     3841 gcggctctcg cgatcagttg ctcggcccgc ggccgtcgcg gcgactcaag cgcgcgacat
     3901 ccctcgaacg tctggtgacc ccggatgcgc cgcccatgtt catctggacg accggcgaag
     3961 acaagtcggt cgccatcgcc gatcactcct acacgcttgc gacggcgctg gcccgcaacg
     4021 cggtgccgca cgacttccac gtcttcgagc gcggcgccca cggcctcggc ttcgccgagg
     4081 gcatccccgc cgaagcctgg cgcgggctcg ccgagcgctg gctcgagtac cgccgctagc
     4141 gcatcggctc cagcacgaac accgggatct gccggtcggt cttctcctga tacgtcgcgt
     4201 agtcgggcca cgcctcgacg gcgcgctccc accacacggc cttctcgtcg cccgtgacct
     4261 cgcgggccag gtagtcgttc ttgtcggggc cgtcctgcag ctcgacgtgc ggattcgcag
     4321 tgatgttgaa gtaccagacc ggatgcttcg gcgcaccgcc cagtgacgcg acgaccgcgt
     4381 acaccccatc gtgctccacg cgcatcagcg cggtcttgcg cagcttgccg gtcttcgcgc
     4441 cgacgctggt cagcacgatc acgcgatggc cgcgaaggtc gacgccttcg gtgccgccgg
     4501 atgcctcgta cagctccgcc tgcttgcgcg cccagtcgga cgtgctcggt tcgtattctc
     4561 ctgtgagtgg catgtagaca tacaaccccc tcgccgccga cggcattcca cgcgaccggc
     4621 ggggtgggct gattcgcgct aacgtaggag ctgtcagacg ccccgcgtcg ttgcccgaaa
     4681 aaggggcaat aagcatcgag ttcgctcgcg agacatatac ggacaccctt ccgtttcaga
     4741 tcttgagagc accgatgcct tctgtttctg cggtcattgc gacgaccctg tcagcccatg
     4801 tccacgacgt cttcggagtg atgggcaacg gcaacgccta cttcctcgac gctctggaga
     4861 ccacgtccac ggtcttcact cccgtgcgtc acgaggcggc cgccgtcgcc gccgccgacg
     4921 cctactttcg cgcctgcggg cggctggccg cggccaccgc gacctacggg gctggtttca
     4981 ccaacctcat caccccgctc gccgaggcgg tgcgcgcgcg catccccctc gtcgtgatcg
     5041 tcggctccgc cccggagtcg ggaatgcggg actgggacgt cgaccaaacc gccctcgcct
     5101 ccgcggtcgg tgccgccacg atcaccgtgg acaggatgga cgcgcagggc cagaccgaac
     5161 gggcagtcgc gctcgcccgg tcgacccgca tgcccgtggt ccttgctgtt ccctacgaca
     5221 tcggccgcgc cagcgtcgcc gatccggacg ctgcaccgac ctcctgggtt gcggatgacg
     5281 cccccacagc acccctcgac gccgacgaga tcgcggctgc cgccgccgcg ctcgccggtg
     5341 ccgcccggcc actgatcctc gccgggcggg gcgcttggct cgctcaggcg ggagaagtgc
     5401 tcggtgaact cgccgactgc gtgggcgccc tgactgcgac atccgcactc ggagcgggtc
     5461 tcttcggtga ctcgccctgg aacctcggca tcgcgggtgg cttcggcagt cacgagtcag
     5521 ccgccctcat gcacgaggcc gatgtggtgc tcgtcgtggg tgcccggctg aaccagttca
     5581 cgatgcggtt cggatcgttg ctcgatccgg cggccacggt catccagatc gataccgacc
     5641 ccgagtcacg ccacccggtg accgacctgc ttctcgttgg agacgccgtc gacgtcggtc
     5701 gagccctgtg cgatcggctg gcggagcatc gagcgatcac gccgtggcgg gccaccgcag
     5761 gtgaggtgcc gcacggcctg agctcgatgg tcggtgaggt cgcggtgctc gaggatgcgc
     5821 ggctcgaccc ccgcaatgtc ttccagcgtc tcgaccgcgc gctgccgctc gatcgggtgg
     5881 tggtgcagga cggcggacac ttcatcggct gggctccggg gtatctgcgc attccggcgc
     5941 cgaaccgctt gatcatggtc ggcacagcgc tccagacgat cggtctcggg ttcgccagcg
     6001 gtgtcggtgc ggccgtggcg gcacccgact ccaccatcgt tctggcgacg ggtgacggtg
     6061 gcgggctgat ggcgctctcc gacctccagt cgttcatcgc cgcgacccgg cggggtgtgg
     6121 tcatcgtggt gaatgacgct ggctacgggg cggagatgca ccaatacggt tcgaagggcc
     6181 tctccgcgac ccccatgctg atcgacgacg tcgacttcgc cgctgtcggc actgctctgg
     6241 gcgcccacgg actcaccgtg cgcaccctcg acgacctcga cgctcttgag cgatggctgg
     6301 ccgacggatc cgacggagtg tgggtcgtcg actgcaaggt ctcccagtcg atcatcgcgc
     6361 cctacatgcg cgagatcatg gtgaccggga tgtagcgaat caggggcgca gctgtccttc
     6421 cgtgtaccgg ctcggaggcg ttccgtgcac ggcggagaac acccgggaga agtacgcggg
     6481 gtcgctgaac cccactcgtc tggcgacctc ggtgacgcga agccccgttg tgcgcagcag
     6541 gatcgccgcc tcctgcatcc tgcgatggcg ggtccaggac acgacggagt ggccggtgta
     6601 gcgcgagaac agacgctcgg ccgttgccgt gctgcagtcg ccgatgcggg cgacctcggc
     6661 gacggtgagc ggctgatcca gtcggctgag gatgtgctcc gtcatcgcct gcagggcgac
     6721 cggcgtagcg gtgggctcgg tttcgcgcgc gggctcgagt ccgctctcct cggcgatcag
     6781 cccgcccagc gcacgcaggg ccggctcctc ggtgcggccg gtcaggaacc gttcgacgca
     6841 gtacgaggcg agcccgatga tgctccgacc cggcacggag cgactcggca gctcggccgc
     6901 gatcccgggc tctgccggcc cccggcgcca gggcacaccg agcagtgggt cgtcggcccg
     6961 gaacgccacc cgcggctcca cggcaaccgc caggctgtgc caggggacga ggtgaacagt
     7021 gccgatgtga agcggcgtcg taccgtgggg ctcgtagtcg acgtcgtgac cccacggcag
     7081 tcgcagaacg gtgctggtgg agagggagtg agtacgccca ccggtcgtca cgcttcccga
     7141 gccctgcacc acccagagga agcacacgct ctcgatgcgg gccacacgga tgcgctggtc
     7201 cacccggaag cggtaccagt tggcgccgat gacaacagga ccaccggctt cgggc
//
LOCUS       DAOSOU010000093         7198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10400_length_7198_cov_6.591208, whole genome shotgun sequence.
ACCESSION   DAOSOU010000093 DAOSOU010000000
VERSION     DAOSOU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10400_length_7198_cov_6.591208"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1562)
                     /locus_tag="PLW15_06450"
     CDS             complement(273..1562)
                     /locus_tag="PLW15_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT34069.1"
                     /translation="MSELQPQPEMRSRSELRVRGGSSAPGLARHGRLRKASPWGTIAK
                     FVAAALAVILVSGASVGAIAFNSIYSKRTVVALVGETEGPPPQIGAIEGGFNILIVGS
                     DTRGGQYGIGGDVADDSSVLNDVNILLHVSQDQTNAVAISFPRDMVVGIPECPWDDGS
                     GDTKGYSTEPINVALYYGGLPCVVLTVEELTGLPIQFAGMITFDGVVDMSDAIGGVDV
                     CITAPMYDPYTGIDLPTAGTWKLSGFNALAFLRSRHGVGDGSDLTRISSQQVYLSSLV
                     RTLKSQDTLGDVKKLYNLANAAVSSMTLSSSLADVSTMVSIALALKNIPLERVTFVQY
                     PGNTGGDGIYAGKVRPDTATGDEMMALIAADQPFVLAETTDGRGSTIDPNAPVATPDP
                     TAAPVDNSGLPVIEGVKGQTAADYTCSITQVSSGDED"
     gene            complement(1605..1689)
                     /locus_tag="PLW15_06455"
     tRNA            complement(1605..1689)
                     /locus_tag="PLW15_06455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Ser,seq:tga)
     gene            complement(1759..2559)
                     /locus_tag="PLW15_06460"
     CDS             complement(1759..2559)
                     /locus_tag="PLW15_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOT34070.1"
                     /translation="MTDRWLIALDVDGTVLREDGVMNDPVVQAVGRVRDAGHAVMLAT
                     GRSVAMTLPILERLGIEPEYVVCSNGAITLKRDPDAVMGYSRFHVETFDPSEVLTTIR
                     GNLDSANYAVEDEHGHYRYTGYFPDGTLGAVSEKVSFEELLITHATRVVVISPEHKIE
                     DFLSVVEKMGLHKVSYNVGWTAWLDIAPDGVNKATALERVRELLGVPRSRVMAVGDGR
                     NDIDMLEWASAEGRGVAMGQAPEEVRAVANEQTLSDLDDGVAVLLSAF"
     gene            complement(2556..3821)
                     /gene="serS"
                     /locus_tag="PLW15_06465"
     CDS             complement(2556..3821)
                     /gene="serS"
                     /locus_tag="PLW15_06465"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT34071.1"
                     /translation="MIDPQLLRDDPEAVRRSQESRGASVDIVDKAIAADGARRASIGE
                     FESLRAEQNAFGKTVAAAPKEQKQALVAEAQQLAARVKAAQQVAQDAETEFTAIAGSI
                     ANVVIDDVPAGGEDNFVTLREVGTKATFDFEPRDHAELGELLGAIDIARGVKVSGSRF
                     YFLKGVGARLELALMNLALDKALAEGFIPLITPTLVRPEIMAGTGFLGEHSDEIYYLP
                     ADDLYLTGTSEVALAGYHSDEILDLSAGPLRYAGWSTCYRREAGSAGKDNRGILRVHQ
                     FNKLEMFTYVLPEQAQEEHERLVGWQESMLQACGLSYRVIDVAAGDLGSSAARKFDIE
                     AWVPTQGTYRELTSTSNCTTYQARRLDIRYRTESGKTTPVATLNGTLATTRWLVAILE
                     THQQADGSVVVPDALRPYLGGLELFEPQS"
     gene            3861..4844
                     /locus_tag="PLW15_06470"
     CDS             3861..4844
                     /locus_tag="PLW15_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOT34072.1"
                     /translation="MTNPRDGKKNRAAVVYNPVKVELKKLRKSVTAATKSAGWAETVW
                     FETSEDDPGQGVTASALRRGADVVLAAGGDGTVRAVAEALRGTGVPLAVLPSGTGNLL
                     ARNLDLPLSNLDASVGAAFTGDAHPIDLGVAEITRPDGVTSEHAFLVMAGLGLDAKMI
                     KNTSTRLKKAVGWLAYIDGIARSLPELKPVRVHFRLDREIVRSMNVHTIIVGNCGVLP
                     GGFLLMPDAKPDDGILDVAALRPRGPFGWAKVWQKVAWENGVLRKSAIGRKIIDLSKD
                     VRDVTYFTTRDLTMTVDVPQEFQLDGDEFGVAASVHTWVDAGALTVMVPRG"
     gene            5231..5905
                     /locus_tag="PLW15_06475"
     CDS             5231..5905
                     /locus_tag="PLW15_06475"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOT34073.1"
                     /translation="MLWGAPLPPHVEREQAVHVSVPAPESAPHARGIRGHSTTRLPGD
                     VATRSGVAVTSPARTWCDLAAVLDLGDLVAMGDYLVSRRLPLVTIAEIRGRLALIGSG
                     RGIRMARRAVDLVRDASESRPESRLRVILAVAGLPEPTINHTLVDTETGKHLRPDFLF
                     AEAKVILEYQGDYHRSRSQWRKDMTRRSRLEAQGWYVMEINADDLGDPVELVARIRSV
                     LARRAR"
     gene            complement(6026..6955)
                     /gene="pheA"
                     /locus_tag="PLW15_06480"
     CDS             complement(6026..6955)
                     /gene="pheA"
                     /locus_tag="PLW15_06480"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOT34074.1"
                     /translation="MPDETYSYLGPAGTFTEAALKQVPEAQGKQWRSVNNVGEALDDV
                     ISGRSTAAMIAIENSIEGGVSVAQDALATIPGLRIIGEYLVPVNFVLVARPGTALADV
                     KVINAHPVAYGQTHRWLDEHLPEHGHIPATSNVAAAASLFETDQADAAVAPPGITEHH
                     DLTVLAEGIGDNPNAVTRFVLVSRTAQLPEPTGSDKTSIIAELPDDTAGRLLEMLEQF
                     ATRGVNMSLLESRPIGDALGRYRFVIDLDGHILDERVADALLGLKRFSPHVIFLGSYP
                     RADKQRITVVERYDNDAFVDARDWLRGLIAGER"
     gene            6990..>7198
                     /locus_tag="PLW15_06485"
     CDS             6990..>7198
                     /locus_tag="PLW15_06485"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192631.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HOT34075.1"
                     /translation="MNTRAGQPAQPSDLIDVHELIAAYFEKVPDVSIPTQRVVFGTSG
                     HRGSSLDTAFNDTHIAAITQAIVEYR"
BASE COUNT         1156 a         2388 c         2437 g         1217 t
ORIGIN      
        1 gacggtggga tttgaaccca cggaggagtt taaacccctc tccaccttag cagggtggtg
       61 cactaggccg aactatgcga cgtctccagt aacgtcagca atcatagctg gaacccacgg
      121 gatgtcagga atcgggcatc cgagcagccg gggctggttc gcctcttgtc ctccaaaagg
      181 tggacaccta agatcgactc acggtatacc cgatgccgac ccgtgtctgg ctggctcctg
      241 agcgacgact cggagccagc cagctccacg aactagtcct cgtcgccgct ggacacctgc
      301 gtgatagagc aggtgtagtc ggccgcagtc tgacccttca ccccttcaat caccggcagt
      361 ccactgttgt cgaccggcgc tgccgtcggg tcaggcgtcg cgaccggcgc gttcggatcg
      421 attgtcgagc cgcggccatc cgtcgtctca gcgagcacga acggctgatc agccgcgatg
      481 agggccatca tctcgtcgcc cgttgccgta tcggggcgta ccttgcccgc gtagatgccg
      541 tcgccgccgg tgttgcccgg gtactgcacg aacgtcaccc gctcgagagg gatgttcttg
      601 agcgccagag cgatcgacac catggtcgac acatccgcca ggctgctcga cagcgtcatg
      661 ctgctgaccg cggcgttcgc caggttgtag agcttcttca cgtcgccgag agtgtcctgg
      721 ctcttgagag tgcgcacgag cgaggagagg tacacctgct gcgagctgat gcgggtcagg
      781 tcgcttccgt cgccgacgcc gtgccgcgag cggaggaagg cgagagcgtt gaagcccgac
      841 agcttccacg tgccggccgt gggaaggtcg atgcccgtgt acgggtcgta catgggggcc
      901 gtgatgcaga cgtcgacgcc accgatggca tcggacatgt caactacacc gtcgaacgtg
      961 atcatgccgg cgaactggat aggcaggccg gtcagttcct ccacggtgag caccacgcag
     1021 ggcaggccac cgtagtagag cgccacgttg atgggctcgg tcgagtagcc cttggtgtca
     1081 ccggaaccgt catcccacgg gcactcgggg attccgacga ccatgtcgcg ggggaagctg
     1141 atggcgacgg cgttggtctg gtcctgcgag acgtgcagca ggatgttcac gtcgttgagc
     1201 accgaactgt cgtcggcgac atcgccaccg atgccgtact ggccaccacg ggtgtcgctg
     1261 ccgacgatca ggatgttgaa gccaccctcg atcgcaccga tctgcggcgg cgggccctcg
     1321 gtctcaccca ccagggcaac aaccgtgcgc ttcgagtaaa tgctgttgaa cgcgatagca
     1381 ccgacggatg ccccgctcac gaggatcacg gccagtgcgg ccgcgacgaa cttggcgatc
     1441 gttccccacg ggctcgcctt gcgcagtcga ccgtgccgag ccagaccggg cgcggaacta
     1501 ccgccgcgca cccgcagttc tgatcgggag cgcatctccg gttgcggttg caactcgctc
     1561 atggaagttc ctttcgtcac ccgacgggac gtgatctata gacgcggagg gcgtgggatt
     1621 cgaacccacg aatgctttca catcaccagt tttcaagact ggctccatcg gccgctcgga
     1681 cagccctccc gaaacccaag gtccgagtgt atccgatgcc cgacgccgga gcctgtagca
     1741 gggtaccgac ggcggctgtc agaaagccga gagcagcacg gccacgccgt cgtcgaggtc
     1801 gctgagggtc tgctcgttgg cgaccgctcg cacctcttcg ggcgcctgac ccatagcgac
     1861 tccccggccc tcggccgacg cccactcgag catgtcgatg tcgttgcgac cgtcaccgac
     1921 cgccatgacg cgggagcgcg gcacccccaa cagctcacgc acgcgctcga gcgccgtcgc
     1981 cttgttgacg ccgtcgggag cgatgtccag ccacgccgtc catcccacgt tgtagctgac
     2041 cttgtgcaga cccatcttct ccacaacgga gaggaagtcc tcgatcttgt gctcggggga
     2101 gatcacgacg acccgggtcg cgtgcgtgat gaggagctcc tcgaagctca ccttctcgct
     2161 cacggcgccg agggtgccat cggggaagta gccggtgtac cggtagtgcc cgtgctcgtc
     2221 ctcgacggcg tagttggccg agtcgaggtt gccccgaatg gtggtgagca cctcgctcgg
     2281 gtcgaaggtc tccacgtgga agcgcgagta ccccatcacg gcgtcgggat cgcgcttgag
     2341 cgtgatcgcg ccattcgagc agacgacgta ctcgggttcg atgccgagcc gctcaagtat
     2401 cggcagcgtc atcgccacgg agcggccggt ggcgagcatc acggcgtgac ccgcatcgcg
     2461 cacccggccg acagcctgca ccacgggatc gttcatcacg ccgtcctcgc ggagcaccgt
     2521 gccgtccaca tcgagcgcga tgagccagcg gtccgtcatg actggggctc gaacaactcg
     2581 aggccgccga ggtaggggcg cagggcatcc ggaaccacga ccgagccgtc ggcctgctgg
     2641 tgggtctcga ggatcgcgac cagccaacgg gtggtggcga gggttccgtt gagggtggcg
     2701 accggggtcg tcttgcccga ctcggtgcgg taccggatgt cgagtcgccg ggcctgatag
     2761 gtggtgcagt tgctcgtgct cgtgagctcg cggtaggtgc cctgggtcgg cacccacgcc
     2821 tcgatgtcga actttcgggc ggcgctcgaa ccgaggtcgc cggccgcgac gtcgatcacg
     2881 cggtagctca gcccgcaggc ctgcagcatc gactcctgcc agccgacgag gcgctcgtgc
     2941 tcctcctgcg cctgctcggg cagcacgtac gtgaacatct ccagcttgtt gaactggtgc
     3001 acccgcagga tgccccggtt gtccttgcca gcggagcccg cctccctgcg gtaacaggtc
     3061 gaccacccgg cgtagcgcag ggggcccgcg gacaggtcga ggatctcgtc ggagtggtat
     3121 ccggcgagcg ccacctcgct cgtgccagtg aggtagaggt cgtcggcggg taggtagtag
     3181 atctcgtctg agtgctcacc gaggaatccg gtgcccgcca tgatctcggg tcgcacgagc
     3241 gtcggggtga tgagcgggat gaacccctcg gccaacgcct tgtcgagggc gaggttcatg
     3301 agcgccagct cgaggcgggc gcccacgccc ttgaggaagt agaaccgact gcccgacacc
     3361 ttcacgccac gagcgatgtc gatcgcgccg agtagttcgc caagctcggc gtggtcgcgc
     3421 ggctcgaagt cgaaggtcgc cttggtgccg acttcacgca gggtgacgaa gttgtcctcg
     3481 cctccggcgg gcacgtcatc gatgacgaca ttggcgatcg agccggcgat cgcggtgaac
     3541 tcggtctcgg catcctgtgc cacctgctgg gcggccttca ctcgcgcggc cagctgctgc
     3601 gcctcggcga cgagggcctg cttctgctct ttcggggccg ccgcgacggt cttgccgaac
     3661 gcgttctgct cggctcgaag gctctcgaac tcgccgatcg atgcgcgtcg agcaccatcg
     3721 gcggcgatgg ccttgtcgac gatgtcgacg gatgccccgc gcgactcctg cgagcgcctc
     3781 actgcctcgg ggtcgtcacg gagcagctga gggtcgatca cccgtacagc ctacccagcg
     3841 gatgccgtac cgttggcaga gtgacgaacc ctcgggatgg gaagaagaac cgcgcggcgg
     3901 tcgtctacaa ccccgtgaag gttgagctca agaagcttcg caagtcggtc actgcggcca
     3961 ccaagtcggc gggctgggcc gagaccgtgt ggttcgagac cagcgaggat gacccgggcc
     4021 aaggagtgac ggcgagcgcc ctgcgacgcg gcgccgatgt ggttctcgcg gccggtggcg
     4081 acggcacagt gcgggccgtc gcggaggcgc tccggggcac cggcgttccc ttggccgtgc
     4141 tgccgtcggg aaccggcaac ctgctcgccc gcaacctcga cctgccgctg tcgaatctcg
     4201 atgcgagcgt cggtgccgcc ttcaccggtg acgcccaccc gatcgacctg ggcgttgccg
     4261 agatcacccg cccggacggc gtgaccagcg aacacgcatt cctcgtgatg gcggggctcg
     4321 ggctcgacgc caagatgatc aagaacacct cgacgcgcct caagaaggcc gtcggctggc
     4381 tcgcgtacat cgacggcata gcccggtcgc ttccggagct gaagcctgtg cgcgtgcact
     4441 tccgcctcga tcgcgagatc gtgcgctcga tgaacgtgca cacgatcatc gtgggcaact
     4501 gcggggtgct ccccggcgga ttccttctca tgcccgacgc caagcccgac gacggcatcc
     4561 tcgacgtcgc cgccctgcgc ccccgcggac cgttcggctg ggcgaaggtc tggcagaagg
     4621 tcgcgtggga gaacggggtg ctgcgcaagt cggcgatcgg gcgcaagatc atcgacctgt
     4681 ccaaggacgt acgcgacgtc acctacttca cgactcgcga cctgaccatg acggtggatg
     4741 tgccgcagga gttccagctc gacggcgacg agttcggggt cgcggcatcc gtgcacacct
     4801 gggtggacgc gggcgcgctc accgtgatgg tgccgcgggg ctagcgtcac ccctcacccc
     4861 cctcgccgcg cccccacccc cccttcgccg agttcgcctg gatgaccagt caggggcgtc
     4921 atccattgga catctaggcg atctcggcgc gagcattcgc tcggagctgt ggatgacggc
     4981 tgcgcgcgac cgcagggttc gtgaggctga aggcgtgaag cgcacaccgt tgccgcctgc
     5041 cctcgctgcg gcgccttttt ccgttgctgg ctcgggtctg tcgcgaaaca ggctccgggc
     5101 ggctgacctc gaccgctcga tctacgggat gcgcgtgaac gcgaaccaac ccctggatct
     5161 agttacgcgc tgtcgcacca tcacactcag aattcgggat gacgcgatcg tcagtcacgc
     5221 aaccgcggcg atgctctggg gcgcacccct gccgccccat gtggagcgcg agcaggcggt
     5281 gcatgtttcg gtgccggcac ccgagtcggc gccgcatgca cggggcatcc gcggtcactc
     5341 cacaacgagg cttccgggcg acgtggcgac ccgatcgggc gtcgcggtga cctcgccggc
     5401 gcgcacctgg tgcgacctgg cggcggtcct cgacctgggg gacctggtcg cgatgggcga
     5461 ctacctcgtg tcacgacggt tgccgctggt gacgatcgct gagattcgcg gccgcctcgc
     5521 gctcataggc tcgggtcgcg gcatccggat ggcccggcgt gcggtcgacc tggtgcgtga
     5581 tgcatccgag tcgcgacccg agtctcggct cagggtgatt ctggctgtcg cggggctgcc
     5641 cgagccgacc atcaaccaca cgctcgtcga caccgagacc ggcaagcacc tgcggcccga
     5701 cttcctgttc gccgaggcga aggtgatcct cgagtaccag ggtgactacc accgcagcag
     5761 gtcacagtgg cggaaggaca tgacgcgccg ctcacggctc gaggcgcagg gctggtacgt
     5821 gatggagatc aacgcagacg atctcggaga tccggtggaa ctcgtggctc gaatccgctc
     5881 cgtgctggcc cggcgggcac ggtgatctgc tccgagttcg cctagatggc gggttgggga
     5941 cgcggcacac cgtgcatcta ggcgaactcg gcgagaaggg gtggggtggt ggggtgtggc
     6001 gcgggtgggg ccaggggagt gcgcgctagc gctcgcccgc gatcagcccg cgcagccagt
     6061 cgcgcgcgtc gacgaacgcg tcgttgtcgt agcgctccac gaccgtgatg cgctgcttgt
     6121 cggcgcgcgg gtagctgccg aggaagatca cgtgcgggct gaagcgcttg agtccgagca
     6181 gggcgtccgc gacccgctcg tcgaggatgt gcccgtcgag gtcgatgacg aagcggtagc
     6241 gtccgagggc gtcgccgatg gggcgcgatt caagcaggct catgttgacg ccgcgggtgg
     6301 cgaactgctc gagcatctcg agcaggcgtc cggccgtgtc atccggaagc tcggcgatga
     6361 tcgaggtctt gtcggagccc gtgggttccg gcagctgcgc cgtccgcgac accagcacga
     6421 agcgggtgac cgcgttcggg ttgtcgccga taccctcagc gagcacggtg aggtcgtggt
     6481 gctcggtgat gccggggggt gcgaccgcag catccgcctg gtcggtctcg aacagggatg
     6541 cggcggccgc cacgttcgac gtcgccggga tgtgcccgtg ctccggcagg tgctcatcga
     6601 gccagcggtg ggtctgtccg taggcgaccg gatgcgcgtt gatgaccttc acgtcggcga
     6661 gcgcggtgcc gggccgggcc accagcacga agttgacggg cacgaggtac tcgccgatga
     6721 tgcgcaggcc cgggatcgtc gcgagcgcgt cctgtgcgac gctcacgccg ccctcgatcg
     6781 agttctcgat cgcgatcatc gccgccgtgc tgcgacccga gatgacgtcg tcgagcgcct
     6841 cgccgacatt gttcaccgag cgccactgct taccctgcgc ctccggcacc tgcttgagcg
     6901 cggcctcggt gaaagttccg gccggcccga ggtagctgta ggtctcgtca ggcatggtca
     6961 ccactgtact ggcgtgccac tatcgaccca tgaacactcg cgcgggacag ccggcccagc
     7021 cctccgacct catcgacgtg cacgaactca tcgctgccta cttcgagaag gtcccggatg
     7081 tctcgatccc cacccagcgc gtcgtgttcg gcaccagcgg ccaccgtggc tcctccctcg
     7141 acaccgcgtt caacgacacc cacatcgccg cgatcacgca ggcgatcgtg gagtaccg
//
LOCUS       DAOSOU010000094         7187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10420_length_7187_cov_8.395962, whole genome shotgun sequence.
ACCESSION   DAOSOU010000094 DAOSOU010000000
VERSION     DAOSOU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10420_length_7187_cov_8.395962"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLW15_06490"
     CDS             complement(<1..324)
                     /locus_tag="PLW15_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOT34076.1"
                     /translation="MSTVPLKPVTADFVLADVDTSSHPGVDKKSAEKELLSNHDTLAD
                     LQERLFAESKFGGTRSVLLVLQAMDTAGKGGIIRHVVGGIDPQGVHIHAFKAPTEEER
                     QHDFLW"
     gene            complement(335..2017)
                     /gene="menD"
                     /locus_tag="PLW15_06495"
     CDS             complement(335..2017)
                     /gene="menD"
                     /locus_tag="PLW15_06495"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOT34077.1"
                     /translation="MTPRPPESPATAFAVALLEEFHRLGVRDIVLGPGSRSQALALAA
                     AEFERLGMIRLRVRIDERVGGFLALGIAVETSRPVILITTSGTAVGNLHPAVLEAHHS
                     GVPLIVITADRPESLRGVGSNQTTEQPGIFSSAVRRSWDVAPPNGSAGETDAAAALAR
                     DALAAASGPVHLNLAFAEPLSSPFTLAERPLPEPVSVVELPVAEALEAAPSSASGTVG
                     LQLSAVPGTVVVAGTGAGPRAEEVARELGAPLLAEIASGAHFGPQLVVAYRELLNTAN
                     FGDRVQRVIVFGHPTLSREVPALIQRQGVETIVVRGASAEDYNPGRRVGTFADSVSVV
                     GEPDARSWSGRWVAASRALIEDIDVAPDITADLGTHAKAELAAVRAPVTRRALVEAVW
                     RATWPKDRLVLGASRLIREADKVLPGKRVPVHANRGLAGIDGTISTALGMALASQAEG
                     AEGTTRVLVGDLTFLHDVGALLFGVGEVRPRIQVIVGNDNGGTIFDGLEVAQSAGPGY
                     DRVLLTPQVVDIPKLAEAYGWDYLRVETRGALDQAMTASLRPVIVEVPLLRE"
     gene            complement(1998..2327)
                     /locus_tag="PLW15_06500"
     CDS             complement(1998..2327)
                     /locus_tag="PLW15_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HOT34078.1"
                     /translation="MGRLLIFFVAAFILDVFALIDLILIDRRRVRAFPKIVWGIIIVL
                     VPFIGALLWFFVGHEWGTRGGERRTIAPDDDPSFLKNLRRDEDQDERIRRLEQELADL
                     DDDPPTT"
     gene            2382..2621
                     /locus_tag="PLW15_06505"
     CDS             2382..2621
                     /locus_tag="PLW15_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HOT34079.1"
                     /translation="MKPWVLYSLVRVGVFAIALAVLLIVGVPWWLAAIVAAVIGLCVA
                     YIFFGKLRDAVALDIADRRSRTTKDADQIAEDAEL"
     gene            complement(2618..3610)
                     /locus_tag="PLW15_06510"
     CDS             complement(2618..3610)
                     /locus_tag="PLW15_06510"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOT34080.1"
                     /translation="MASQLKAEKARQQAAQKGKKKSGRPGLPPGVASVRKATLGDWIG
                     GMRLQTLPLAIAPVALGTATAYVLPHEETGDGWHWLRALACLAVAVFLQIGVNFANDY
                     SDGVRGTDKFRSGPKRLTASGAAKPRTVLTVALVFFALAAAAGLVIVWRTGYWWLIAV
                     GAVCILASWFYTGGKRPYGYAGLGELFVFVFFGIVATAGTTFVLAGTVTVESWLAGAA
                     AGFIACAVLMVNNIRDLAQDKLAGKRTLAVRLGDRPARIAYAVLLLLPFVILVFFTLF
                     YANAYLVYFALLAAVPAAGITLSAKTAPELVLALRLTALTALAYGLGLGWAIAF"
     assembly_gap    3653..3752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3753..4624)
                     /locus_tag="PLW15_06515"
     CDS             complement(<3753..4624)
                     /locus_tag="PLW15_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT34081.1"
                     /translation="MTEVFDALRDALAGDGPAILPRPSSVSSADDISTSLDDRTIEQR
                     VAVVVETSGSTARPKRVMLSADALLASAAASETALGTSGQWLLALPTHYIAGLAVLVR
                     SLAAGTDPVVLDPRGFTPEAFVAAAARLDHPTRFVSLVPAQFARLMASDAAVDALRGF
                     EGILVGGQSTPVALAAAAVDKGLRLTRTYGASETCGGCVYNGVPIGNTEIAIVDGRIE
                     LSGSVIAEGYLDDPRRTAFSFPEHDGRRWYRTDDTGAMHHGRLAVTGRIDDVIISGGI
                     KVSLAEVESVVRDQL"
     gene            complement(4724..5344)
                     /locus_tag="PLW15_06520"
     CDS             complement(4724..5344)
                     /locus_tag="PLW15_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34082.1"
                     /translation="MSVADWPHLFVAGDPGSPVIVALHGTGGTEHDGVALAREIAPGF
                     AVLAPRGRVSEQGMNRWFRRLAEGVFDVDDVEARADELAAFVAEAAAEYDLSGRMLVV
                     AGFSNGANMALATAIRHPQLLTRAIGFSGMYPFADRTPTDSLAGLEVLLLNGHDDPMA
                     PRASVDRLHTVLTGLGAESTREERAGGHGISAAELETAREWLAAHA"
     gene            complement(5341..6312)
                     /locus_tag="PLW15_06525"
     CDS             complement(5341..6312)
                     /locus_tag="PLW15_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HOT34083.1"
                     /translation="MTVMTQGLHHVTAIAGAPQRNIDFYITGLGLRLVKKTVNFDDPG
                     TYHLYYGDEAGRPGSLITFFPWRGIQQGRVGAGQSTSTAFSVPAGSLGWWTEHFAKIG
                     AESRITSESSSEERLLVSDPDGLQIELVASSVTDPRDPWDSASVPAEYAIRGQHSSVL
                     TVRHPEQTIALLTNDLGLTIVDESASRTRLAAGDGSAGTIVDVIGSSLAPDGLGAGGT
                     VHHIAFRVPDHATQAAWREELVGKGHQVTGILDRQYFTSIYFREPGGVLFEIATDTPG
                     FDIDEPLLELGRSLKLPPWLEPSREQIEHSVAQITLPEENNPALGAA"
     gene            complement(6376..6777)
                     /locus_tag="PLW15_06530"
     CDS             complement(6376..6777)
                     /locus_tag="PLW15_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34084.1"
                     /translation="MADPGEVERVPLKVVPIFIDEPVVSEPIETPDAPPPAPRRKTAL
                     LGATALAAAVIAGVLQGVAIAVATGGDYFAATVLGYLSIGLAVLAVVGGVVAIILDRG
                     RRLGIAAVALGVLANPFVLLILFQLVGTLTT"
     gene            6820..>7187
                     /locus_tag="PLW15_06535"
     CDS             6820..>7187
                     /locus_tag="PLW15_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylbenzoate synthase"
                     /protein_id="HOT34085.1"
                     /translation="MRPTVTDLLATAHVVSLPLTSRFRGIDVREAMLIEGPEGWTEFS
                     PFTEYGDAEASAWLAAAIDFGWNPAPPALRDTIRVNATMPAVTADDVPAVLARFPGCR
                     TVKIKVAEPGQSLADDTARVA"
BASE COUNT         1148 a         2436 c         2483 g         1020 t
ORIGIN      
        1 ccacaggaag tcgtgctggc gctcctcctc ggtgggcgcc ttgaacgcgt gaatgtgcac
       61 gccctgcgga tcgatgccgc cgaccacatg ccggatgatg ccgcccttgc ctgccgtgtc
      121 catcgcctgc agcacgagca gcacactgcg ggtgccgccg aacttgctct cggcgaacag
      181 acgctcctgc aggtcggcga gagtgtcgtg gttgctgagc aactccttct cggccgactt
      241 cttgtcgacg cccgggtgcg aggaggtgtc gacgtcagcc aggacgaaat cggcggtgac
      301 cggcttcagg ggaacagtgc tcatgcgccc caacctactc gcgcagcagc ggcacttcca
      361 cgatcaccgg acgaagggat gccgtcatcg cctgatccag ggcgccccgg gtctcgacgc
      421 gcaggtagtc ccacccgtag gcctcggcga gcttcgggat gtccaccacc tgcggggtga
      481 gcagcacgcg gtcgtagccg ggaccagccg actgcgccac ctcgagaccg tcgaagatgg
      541 ttccgccgtt gtcgttgccc acgatcacct ggatgcgcgg ccgcacctcg ccgacgccga
      601 acaacagcgc accgacatcg tgcaggaacg tgagatcgcc caccagcacg cgagtcgtgc
      661 cctcggcgcc ctccgcctgg ctcgcgagcg ccatgcccag agccgtcgag atcgtgccgt
      721 cgatacccgc cagaccgcgg ttcgcgtgca cgggaacgcg cttgccaggc agcaccttgt
      781 cggcctcgcg gatgagccgc gaagccccga gcaccagccg atccttcggc cacgtcgcgc
      841 gccagactgc ctcaaccagc gcccgtcggg tgacgggagc gcgcacggcg gcaagctcgg
      901 ccttggcgtg agttcccagg tcggccgtga tgtccggtgc cacgtcgatg tcctcgatga
      961 gggcccggga tgccgcgacc caccggcccg accaggaacg ggcatccggc tcacccacga
     1021 cgctcacgga atcggcgaac gtgcccaccc ggcgccccgg gttgtagtcc tccgcgctcg
     1081 cgccgcgcac gacgatggtc tcgaccccct ggcgctggat cagcgccgga acctcgcggc
     1141 tgagggtggg atgaccgaac acgatgacgc gctgcacccg gtcgccgaag ttggcggtgt
     1201 tcagcagctc gcggtaggcg acgacgagct gcggaccgaa gtgcgcaccg ctggcgatct
     1261 ccgcgagcag cggcgcgccg agctcgcggg cgacctcctc ggcgcggggg ccagcgcccg
     1321 tgccagcgac gactacggtg ccgggaaccg ctgacagctg aaggccgacg gtccctgagg
     1381 cactcgaagg ggccgcttcg agagcctcag cgaccggcag ctcgactacc gacacgggct
     1441 cgggcagcgg gcgttccgcc agcgtgaacg gcgaggagag cggctcggcg aacgcgaggt
     1501 tgagatgcac ggggccggat gccgctgcca gggcatcgcg cgcgagggca gccgcggcat
     1561 ccgtctcgcc cgcggagccg ttcggaggag cgacgtccca cgaacgacgc accgcggagc
     1621 tgaagattcc cggctgctcg gtcgtctggt tcgacccgac accgcgcaac gactcggggc
     1681 ggtcagcggt gatgacgatg agaggcacgc ccgagtgatg cgcttcgagc acggccgggt
     1741 gcaggttgcc gaccgccgtg ccggaggtgg tgatcaggat gaccggcctg ctcgtctcca
     1801 cggcgatgcc gagggcgagg aatccgccca cccgctcgtc gatgcgcacc cgcagtcgga
     1861 tcattcccag gcgttcgaac tcggcggcgg ccagggccag cgcctgcgag cgcgagcccg
     1921 gtccgagcac gatgtcgcgc acgccgagtc gatggaactc ctcgagcaac gccacagcga
     1981 acgccgtggc gggcgactca ggtggtcggg gggtcatcgt cgaggtcggc gagttcctgc
     2041 tccaggcggc ggatgcgctc gtcctggtcc tcatcgcgtc ggaggttctt cagaaaactg
     2101 gggtcgtcat cgggggcgat cgtgcggcgc tcgcccccac gagtgcccca ctcgtgaccg
     2161 acgaagaacc acagcagcgc gccaatgaac ggcaccagca cgatgatgat tccccagacg
     2221 atcttgggga acgcccgcac ccgacggcga tcgatgagga tcaggtcgat gagggcgaag
     2281 acgtcgagga tgaatgccgc aacgaagaag atcagcagcc gtcccatgtg ccaattctag
     2341 gcgcgcacgc tgagctccag cgcgctgcct acactggcag agtgaaacct tgggtgctct
     2401 acagtctcgt gcgggtcggc gtgttcgcga tcgcgctggc ggtgctgctg atcgtgggcg
     2461 tgccgtggtg gctggccgcc atcgtcgccg ccgtcatcgg gctgtgcgtg gcgtacatct
     2521 tcttcggcaa gctgcgcgat gccgtcgcac tcgacatcgc ggatcgccgg tcgcgcacca
     2581 ccaaggatgc cgaccagatc gccgaggacg cggagctcta gaaggcgatc gcccagccga
     2641 gtccgagacc gtaggcgagt gccgtcagcg ccgtgagacg cagcgcgagc acgagctccg
     2701 gtgcggtctt ggcgctcaag gtgatgcccg cggccggcac ggcggccagc agtgcgaagt
     2761 agaccaggta ggcgttggcg tagaagagcg tgaagaacac gaggatgacg aacggcagca
     2821 gcaacagcac cgcgtaggcg attcgggccg gccggtcgcc cagacgcacc gcgagggtgc
     2881 gcttgcccgc cagcttgtcc tgtgcgagat cgcggatgtt gttgaccatg agcaccgcgc
     2941 aggcgatgaa gcccgccgcc gcaccggcca gccagctctc gactgtcacc gttcccgcaa
     3001 gcacgaaggt ggtgccggcg gtcgccacga tgccgaagaa cacgaagacg aagagctcgc
     3061 ccaggcctgc atagccatac ggtcgcttgc cgccggtgta gaaccaggat gcgaggatgc
     3121 acaccgcacc gacggcgatc agccaccagt agccggtgcg ccagacgatc acgagccccg
     3181 cggccgcggc gagcgcgaag aagacgagcg cgacggtcag caccgtgcgc ggcttggccg
     3241 cgccagaggc cgtcaaccgc ttcgggccgg aacggaactt gtcggtgccg cgcactccgt
     3301 cggagtagtc gttggcgaag ttcacgccga tctgcaggaa caccgcgacg gccagacacg
     3361 cgagggctcg cagccagtgc cagccgtcgc cggtctcctc gtgcgggagc acgtacgcgg
     3421 tggcggtacc gagggcgacg ggcgcgatgg cgagcggcag tgtctgcagc cgcatcccgc
     3481 cgatccagtc gcccagcgtc gccttgcgca ccgacgcgac cccgggcggg agtccgggcc
     3541 gtcctgactt cttcttgccc ttctgggccg cctgctgccg ggccttctcg gccttcagct
     3601 gggatgccac gctgggagcg ggtacggact tcgggttgat gcgctgctgc cgnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagctgatc ccgcaccacc gactcgacct
     3781 cggccagcga gaccttgatg ccgccggaga tgatgacgtc gtcgatgcgg ccggtgaccg
     3841 cgaggcggcc gtggtgcatc gcgcccgtgt catccgtgcg gtaccaacgg cggccgtcgt
     3901 gctcgggaaa ggagaaggcc gtgcgccggg ggtcgtcgag gtagccctcg gcgatgacgc
     3961 tgcccgacag ttcgatgcga ccgtcgacga tcgcgatctc ggtgttgccg atgggtacgc
     4021 cgttgtagac gcagccgccg caggtctccg atgcgccgta ggtgcgggtc aggcgcaggc
     4081 ccttgtccac tgcggccgcc gccagggcga cgggagtgga ttgtccgccg acgagaatgc
     4141 cctcgaatcc gcgcagggcg tcgacagcgg catccgacgc catcaggcgg gcgaattggg
     4201 ccggcacgag cgagacgaac ctcgtggggt gatcgaggcg ggccgcggcg gcgacgaagg
     4261 cctccggggt gaacccgcgg ggatcgagca cgaccgggtc ggtaccggcc gcgagcgacc
     4321 gcaccagaac cgcgagcccg gcgatgtagt gggtgggcag cgcgagcagc cactgacccg
     4381 aggtgcccag tgcggtttcg gatgccgctg cgctcgccaa cagtgcatcg gcgctgagca
     4441 tcacacgctt gggtcgcgcg gtcgagccgc tcgtctcgac gacgacggcc acgcgctgct
     4501 cgatggtgcg gtcatcgagc gacgtcgaga tgtcgtccgc gctcgagacg ctcgacggcc
     4561 ggggcaggat ggccggtccg tccccggcga gcgcgtcccg gagtgcgtcg aacacttcgg
     4621 tcacgtctgc cgagtcgacg actcgcaatc cccgcatgga gcccccgctt cctgccgccc
     4681 tcggcgtccg tacaacggta cagccggggg cggcggtgtg gcttcaggcg tgggcggcga
     4741 gccactcccg ggcggtctcg agctctgctg ccgagatgcc gtgcccgccg gctcgctcct
     4801 cgcgagtcga ctcggccccg aggccggtga gcacggtgtg caggcggtcg acgctcgcgc
     4861 ggggcgccat cgggtcgtcg tggccgttga gcaacagcac ctcgaggcct gccagcgagt
     4921 cggtcggggt gcggtccgcg aacgggtaca tccccgagaa tccgatggcc ctcgtcagta
     4981 actgaggatg gcggatcgcg gtggcgagcg ccatgttcgc tccgttggag aagccggcca
     5041 ccacgagcat ccggcccgac aggtcgtact cggccgccgc ttcggccacg aaggcggcca
     5101 actcgtcggc gcgggcctcg acgtcgtcga catcgaacac cccctcggcg aggcgcctga
     5161 accaacgatt catgccctgc tcgctcaccc gacccctcgg cgccagcacg gcgaagcccg
     5221 gcgcgatctc ccgcgccagc gcgacgccgt catgctcggt gccgcccgtg ccgtggaggg
     5281 cgacgatcac cggagaaccg gggtcgcccg ccacgaacag gtgcggccag tcggccacgc
     5341 tcatgccgca cccagcgcgg ggttgttctc ctcgggcagg gtgatctgcg ccacggagtg
     5401 ctcgatctgc tcgcggctcg gctcgagcca cggcggcagc ttgagggagc gacccagctc
     5461 gagcagcggc tcgtcgatgt cgaatccggg ggtgtcggtt gcgatctcga acagcacgcc
     5521 acccggctcg cggaagtaga tcgaggtgaa gtactggcga tcgaggatgc ccgtcacctg
     5581 gtggccctta ccgacgagct cctcgcgcca cgcggcctgc gtcgcgtggt cgggcaccct
     5641 gaatgcgatg tggtgcacgg ttccgccagc cccgagccca tcgggcgcga gcgaggaccc
     5701 gatcacgtcg acgatcgtcc cagcggagcc gtcacccgcg gcgaggcggg tgcgactggc
     5761 cgactcgtcg acgatcgtga gccccaggtc gttggtgagc agcgcgatgg tctgctcggg
     5821 gtggcgcacc gtcagcaccg aggagtgctg tccgcggatc gcgtactcgg cgggcacgct
     5881 ggccgagtcc cacgggtcgc gggggtcggt gacggatgac gcgaccagct cgatctgcag
     5941 accgtccggg tcgctcacca gcagccgctc ctccgaactg gactcggacg tgatgcggga
     6001 ttcggcgccg atcttcgcga agtgctcggt ccaccagccg aggctgccgg cgggaacgga
     6061 gaaggcggtc gaggtcgact gccctgcccc gacgcgaccc tgctggatgc cgcgccacgg
     6121 gaagaacgtg atgagcgagc cggggcggcc cgcctcgtcg ccgtagtaca ggtggtaggt
     6181 gccggggtcg tcgaagttga cggtcttctt cacgagccgc agcccgaggc cggtgatgta
     6241 gaagtcgatg ttgcgctgcg gggcgcccgc gatcgcggtc acgtggtgca gaccctgtgt
     6301 catgacggtc atgatcaaag ttcctttccg gaattcgtta tccatgcaaa cgcatagaaa
     6361 cggaatctat tcccgtcagg tcgtcagggt gccaaccagt tggaagagga tcagcagaac
     6421 gaagggattc gccagcaccc ccagcgccac agcggcgata cccagcctcc gcccgcgatc
     6481 gaggatgatc gccaccacgc cgcccacgac cgcgagaacc gccaacccga tgctcaggta
     6541 gccgagcacg gttgcggcga agtaatcccc acccgtcgcc acggcgatcg ccacgccctg
     6601 cagaaccccc gcgatgacgg cggccgccag ggcggtagcg cccagcagtg cggtcttgcg
     6661 gcgcggggct gggggcgggg catccggtgt ctcgatgggc tcggacacca ccggctcatc
     6721 aatgaagatc ggcacgacct tgagcggaac tctctccact tctcccggat cggccacgag
     6781 aatcacccta accgacccgc accttgccag actggggcaa tgcgccccac cgtgaccgac
     6841 ctgctcgcca ccgctcacgt cgtgagcctg ccgctgacct cgcggttccg gggaattgat
     6901 gtgcgcgagg ccatgctcat cgaaggcccc gaggggtgga ccgagttctc gcccttcacc
     6961 gagtacggcg acgccgaggc atccgcctgg ctcgccgccg ccatcgactt cggctggaac
     7021 ccggcgcccc ctgcgttgcg cgacacgatc cgcgtgaacg cgaccatgcc cgcagtgacc
     7081 gccgacgacg tgccggccgt gctggctcgc tttcccgggt gccgcacggt gaagatcaag
     7141 gtggccgagc cgggccagag cctcgcggat gacacggcgc gcgtcgc
//
LOCUS       DAOSOU010000095         7175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10457_length_7175_cov_6.924017, whole genome shotgun sequence.
ACCESSION   DAOSOU010000095 DAOSOU010000000
VERSION     DAOSOU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10457_length_7175_cov_6.924017"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /gene="glmU"
                     /locus_tag="PLW15_06540"
     CDS             <1..276
                     /gene="glmU"
                     /locus_tag="PLW15_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381109.1"
                     /note="forms a homotrimer; catalyzes the acetylation of
                     glucosamine-1-phosphate and uridylation of
                     N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;
                     function in cell wall synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOT34086.1"
                     /translation="AGTITANYDGVTKNPTIVGDQARTGSHNVFVAPVTIGDGAYTGA
                     GTVVRKDVPPGALAVNVAPQRNIEGWVATNRAGTASAEAAAAASGND"
     gene            338..1315
                     /locus_tag="PLW15_06545"
     CDS             338..1315
                     /locus_tag="PLW15_06545"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOT34087.1"
                     /translation="MSEIKITGQKRLVIVTGRAHPQLAIDIAEELGADLVHTDARTFA
                     NGEIYARYDESVRGCDAFVIQSHTNPINEWLMEQLIMVDALKRASAKRITVVSPFYPY
                     ARQDKKGRGREPISARLVADLYKAAGADRIMSVDLHAAQIQGFFDGPVDHLFAMPVLL
                     EHFQKQLDPSTLTVVSPDMGRVRVADIWSDKLGAPLAIIHKRRDPLVPNQVSVHEIVG
                     QVDGRVCLIVDDLIDTGRTIVKAADALKAAGATKVVVAATHAVFSDPATEILQGASID
                     SVVVTDTLPVPEEKRFPTLTILPIAPLIARAIHEVFDDGSVTSMFDGAA"
     gene            complement(1316..2041)
                     /locus_tag="PLW15_06550"
     CDS             complement(1316..2041)
                     /locus_tag="PLW15_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HOT34088.1"
                     /translation="MDYRVAMRVLEIWRYPVKSLQGERLDSALVGVEGIEGDRQWAIF
                     DVATGFGLTGRREGALLHASARTVDGRVEVVLPDGSVAADDEALSTWIGRPVALRNTD
                     QAESRRFESPEDFENEEAGNWRAFTGALGSFRDDQNAAVSIASTGSLGDWPQRRFRPN
                     LVVSGSGEDDLVGSAIAIGEARLNVQARLGRCVMVTRPQPGIERDLDVLRTIHRERDH
                     TFAVGATIDNVGVITVGDPVEVV"
     gene            2062..3765
                     /locus_tag="PLW15_06555"
     CDS             2062..3765
                     /locus_tag="PLW15_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOT34089.1"
                     /translation="MSISTPRPWLKSYADNVPEEIELPGGSLYDLVAASVTEFGSAVA
                     LEFFGATTTYAEMGQQIDRAAEGLRLLGVQKGDPVALVLPNCPQHIVAFYAVLRLGAI
                     VVEHNPLYTPRELRHQFEDHGARVVIAWDKTVATIQDFPEDVAVETIISVDLTRAMPF
                     TTRALLRLPIAKARESRAALTTKVRGTIEWKKLLDSPPIDERIFRPDAADVALIQYTS
                     GTTGSPKGATLTHLNLTTNAAQARAWVPTVKRGEGCVVYAVLPMFHAYGLTLCLTFAM
                     SMGARLVLFPRFDPDLVLPVIKKRPATFLPAVPPIYERLTAAAREKGVSLKGIQIAIS
                     GAMPLSAAVVEPWEEQTGGYLVEGYGLSETSPVLMANPVGPTRRAGTVGLPLPNTELR
                     VVDPENPTVDRKPGEEGELIVRGPQVFSGYWKKPEETAAVFVPTSDDGADWFRTGDIV
                     TVDSDGFVRIVDRIKELIITGGFNVAPSEVEEALRAHPSIADVAVIGLPSEHSGEDVV
                     AAVVLAPGATLDEAAIREFARANLTAYKVPKRVVVVDELPKSLIGKVLRRKVRESLLD
                     A"
     gene            3853..4890
                     /locus_tag="PLW15_06560"
     CDS             3853..4890
                     /locus_tag="PLW15_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34090.1"
                     /translation="MSALSGAVILGGVLALSAALAPASPASAASTSPARVTPDGETLF
                     AISCDWSGDMSGLQVLALNGATGSGVAIGDPGSPTYFCAIASSWDRSAESCTIYTMAR
                     GADERPALLRTDLISGASTLVTRLTLNGQEEWFDSFTIDGSGAAWATVNGAIYSVDLT
                     TGVLTLASLPDAAGLFDLTWSSYDNEFYAHDVTTVYRVDVVTGSTTSLGPVTLPGGEQ
                     VMMQGMAIDSSGAFWFSRLAPANYESIEDWVRQLVSASPTLTQAEVVGDFRSASGRVG
                     VIAMLSLPEQADCGFELPTLPFREPTPTSPAALAATGASDPAPIVVLAIGTAVAGAAL
                     IGAARLRSRRR"
     gene            complement(4887..5264)
                     /locus_tag="PLW15_06565"
     CDS             complement(4887..5264)
                     /locus_tag="PLW15_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34091.1"
                     /translation="MHDDSQYDDEPELAGYQPHEKPLRSPHLMTVMRVVVVLGLVGLV
                     LPGILVTVGTANRTAQTTCAAYTGFFAPEAVSFSARFELVSAAGIGWNCYAVAFGGAE
                     TLVKAMGLIPGGARLPSAPVENS"
     gene            complement(5341..6099)
                     /locus_tag="PLW15_06570"
     CDS             complement(5341..6099)
                     /locus_tag="PLW15_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT34092.1"
                     /translation="MTSAFDLTGRLAIVTGARRGIGFAMAEALAQAGADIIGVSATLE
                     ASGSAIEQAVTSHGRRFEAIACDFADVDAVAALGERLAVRPVDILVNNAGTIERAPAA
                     EHPIELWDRVIQVNLSSQFVLSQAVGRSMLERGHGKIIFTASMLSFQGGVNVPGYTAA
                     KSGVAGLTKALANEWAGRGVNVNAIAPGYIATDNTQALQDDPARSRAILDRIPAGRWG
                     VGADLGGATVFLASAASDYVSGIVLPVDGGWLGR"
     gene            complement(6096..7118)
                     /locus_tag="PLW15_06575"
     CDS             complement(6096..7118)
                     /locus_tag="PLW15_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT34093.1"
                     /translation="MLTANYVGDNTISIREADAADPGPGEVQIAVAYTGLCGTDLHIV
                     HGTMDARVSTPLIFGHEMSGTIAAIGEGVADWTVGDAVTVMPLDWDGTCPACLAGNQH
                     ICQNLNFIGIDSPGSLQKLWNVSTDVLVRLPEGLRLDHAALIEPVAVAVHDVRRANLA
                     AGEKVVVIGGGPIGVLIATVAREFGGDVTVIELDPHRRGQISELGFTTIDPREVDQVA
                     WVTEWTQNAGADVVFEVSGAAAAVLGATDLAKVRGTIVVVAIHPTPREINLQRVFWRE
                     LSILGARVYQRQDFETAAELLERGAIPADLLITRIVPLSETQAAFADLEAGRAMKILV
                     DVAGQK"
BASE COUNT         1159 a         2507 c         2325 g         1184 t
ORIGIN      
        1 gccggcacca tcacagccaa ctacgacggt gtgaccaaga accccacgat cgtgggcgac
       61 caggcccgca ccggctcgca caacgtcttc gttgccccgg tgaccatcgg cgatggcgcc
      121 tacaccggcg ccggcacggt cgtgcgcaag gatgtcccgc ccggcgccct cgcggtgaac
      181 gtggcacccc agcgcaacat cgagggatgg gtggcgacga accgcgcggg cacggcgtcg
      241 gcggaggctg cggccgcggc atccggaaac gactgaacgc gcccacgtcg cgtcggtaga
      301 atcggtcgca gcgcgtactc aacagagagc agggacagtg tccgagatca agatcaccgg
      361 ccagaagaga ttggtgatcg tgaccggccg ggcacacccc cagctcgcca tcgacatcgc
      421 cgaggagttg ggcgccgatc tcgtgcacac cgacgcccgc accttcgcca acggtgagat
      481 ctacgcccgc tacgacgaga gcgtgcgcgg gtgcgatgcc ttcgtcatcc agagccacac
      541 gaacccgatc aacgagtggc tcatggagca gctcatcatg gtggatgccc tgaagcgagc
      601 ctcggctaag cgcatcacgg tcgtgtcgcc cttctacccc tacgcccgcc aggacaagaa
      661 gggtcgcggt cgcgagccga tcagcgcccg tctggtcgcc gacctgtaca aggcggccgg
      721 cgccgaccgc atcatgagcg tcgacctgca cgccgcccag atccagggct tcttcgacgg
      781 cccggtcgat cacctcttcg cgatgcccgt gctgctcgag cacttccaga agcagctcga
      841 cccgtcaacg ctcacggtcg tgagccccga catgggccgg gtgcgcgttg ccgacatctg
      901 gagcgacaag ctcggggcgc cactggcgat catccacaag cggcgcgacc cgctcgtgcc
      961 caaccaggtc tcggtgcacg agatcgtcgg ccaggtcgac ggtcgggtgt gcctcatcgt
     1021 cgacgacctg atcgacaccg gtcgcaccat cgtcaaggcg gccgacgcgc tcaaggcggc
     1081 gggagcaacc aaagttgtcg tcgcggccac ccacgcggtg ttctccgacc cggcaaccga
     1141 gatccttcag ggcgcgtcga tcgactccgt ggtcgtgact gacacgctgc cggttccgga
     1201 ggagaagcgc ttcccgacgc tcaccatcct gccgatcgct ccgctcatcg cccgcgccat
     1261 ccacgaggtc ttcgacgacg ggtcggtcac cagcatgttc gacggtgccg cctagctaga
     1321 cgacctccac cgggtcaccc acggtgatca cgccgacgtt gtcaatggtc gcgccgacgg
     1381 cgaacgtgtg atcgcgttcg cggtggatcg tgcgcagcac gtcgaggtcg cgctcgattc
     1441 cgggctgggg tcgagtcacc atgacgcagc gaccgagtcg ggcctgaaca ttcaggcgcg
     1501 cttcgccgat ggcgatggcc gagccgacta ggtcgtcctc acctgaaccg gagaccacca
     1561 gattgggtcg gaaccgtcgt tgcggccagt cgccgaggct gccggtcgag gcgatcgaga
     1621 cggccgcgtt ctggtcgtcg cggaacgacc ccagcgctcc cgtgaacgcg cgccagttgc
     1681 cggcctcctc gttctcgaag tcctccggac tctcgaaacg acgcgactct gcctggtcgg
     1741 tgttgcgcag ggccaccggg cggccgatcc acgtcgacag tgcttcgtcg tcggcggcga
     1801 cggacccgtc gggcagcacc acttcgaccc ggccgtcgac ggtgcgggct gacgcgtgaa
     1861 gcagtgctcc ctcgcggcgc ccggtgagac cgaaccccgt cgccacgtcg aagatcgccc
     1921 actggcggtc gccctcgatg ccctcgacac ccaccagcgc ggaatccagc cgctcgccct
     1981 gcagcgactt caccgggtat cgccagatct cgagcacgcg catggcgaca cgatagtcca
     2041 ccgcatccgt agagttgtgc cgtgagcatc agtaccccgc gtccctggtt gaagtcgtac
     2101 gccgacaacg tgccggagga gatcgagctg cccggcgggt cgctctacga cctggtcgcg
     2161 gcatccgtca cggagttcgg cagcgccgtc gctctggagt tcttcggtgc gacaacgacg
     2221 tatgccgaga tgggccagca gatcgacagg gccgcggagg ggctgcggct cctgggggtg
     2281 cagaagggtg acccggtggc cctcgtgctg ccgaactgcc cgcagcacat cgtcgccttc
     2341 tacgcggtgc tgcgcctcgg cgccatcgtg gtcgagcaca atccgctgta caccccgcga
     2401 gagctgcgac accagttcga ggaccacggt gccagggtcg tcatcgcctg ggacaagacg
     2461 gtcgcgacca tccaggactt ccccgaggat gtcgctgtcg agacgatcat ctccgtcgac
     2521 ctcacccgtg cgatgccctt caccactcgg gctctgctgc gcctgccgat cgccaaggcg
     2581 cgcgagtcgc gcgcggctct cacgacgaag gtgcgtggca ccatcgagtg gaagaagctg
     2641 ctggattccc cgccgatcga cgagcgcatc ttccgaccgg atgccgccga cgtcgccctc
     2701 atccagtaca cgagcggcac gacgggcagt cccaaggggg cgaccctcac gcacctcaac
     2761 ctgacgacca atgccgctca ggcacgcgca tgggtgccca cggtcaagcg cggcgagggc
     2821 tgcgtcgtct acgcagtgct gccgatgttc cacgcctacg ggctcacgct gtgcctgaca
     2881 ttcgcgatga gcatgggggc acgcctcgtg ctgttcccga gattcgatcc cgacctcgtg
     2941 ctgccggtga tcaagaagcg tccagcgaca ttcctgcctg ccgttccgcc gatctacgag
     3001 cggctgaccg ccgccgctcg cgagaagggc gtctcgctga agggcatcca gatcgccatc
     3061 tcgggcgcca tgccgttgtc ggctgcggtc gtcgagccgt gggaggagca gaccggcgga
     3121 tacctcgtgg agggctacgg gctcagcgag acgtcgcccg tcctgatggc caaccccgtc
     3181 ggtccgacac gtcgtgcggg aaccgtcggc ctgcccctgc ccaacaccga gctgcgcgtc
     3241 gtcgacccgg agaaccccac ggtcgatcgc aagccgggcg aggagggcga actgatcgtg
     3301 cgcggccccc aggtcttctc cggttactgg aagaagcccg aggagaccgc tgccgtgttc
     3361 gtgccgacct ccgacgacgg ggccgactgg ttccgcaccg gcgacatcgt caccgtcgac
     3421 agtgacgggt tcgtgcgcat cgtcgaccgc atcaaggagc tcatcatcac cggtggtttc
     3481 aatgtggcgc cgagcgaggt cgaggaggcg ctgcgggcgc atccctcgat cgccgacgtc
     3541 gccgtgatcg gcctcccgag cgagcacagc ggcgaggatg tcgtcgcggc cgtcgtgctc
     3601 gccccgggtg caaccctcga cgaggccgcc atccgcgaat tcgctcgcgc caacctcacc
     3661 gcctacaagg tgcccaagcg agtcgtcgtg gtggacgagc ttcccaagtc gctcatcggc
     3721 aaggtgctgc gccgcaaggt tcgggaatcg ctgctcgacg cgtaatgttc cgggtgcgcc
     3781 ggggtttcaa gggcaacgac ccttcctacc cgaggagcac ccgtgtcccc aactcgtcca
     3841 ctgcccggcc gcgtgtcagc actgtccggt gcggtcatcc ttggtggcgt tctcgccctg
     3901 agcgcggccc tcgcgcccgc gtcgcctgcc tctgcggcat ccacatcccc cgcccgtgtc
     3961 acgcccgatg gcgagacgct cttcgccatc tcctgcgatt ggtcgggaga catgagcggc
     4021 ctgcaggtac tggctctgaa cggtgcgacg ggcagcggcg tcgccatcgg cgacccggga
     4081 tcaccgacct acttctgtgc catagcgtcg tcgtgggatc gcagcgccga gtcctgcacg
     4141 atctacacga tggcccgtgg cgccgacgag cggccggccc tccttcgcac ggatctgatc
     4201 tcaggggcta gcactctggt gacgaggctc acgctcaacg gccaggagga gtggttcgac
     4261 tcgttcacca tcgacggcag tggtgccgca tgggccaccg tgaacggcgc catctactct
     4321 gtcgacctca ccaccggagt gctcacgctc gcgagcctcc ccgatgccgc cgggctgttc
     4381 gatctcacct ggagctcgta tgacaacgag ttctacgccc acgacgtgac aaccgtctac
     4441 cgggttgatg tcgtcaccgg aagcacgacc tcactcgggc cggtgacgct gccgggcggc
     4501 gaacaggtga tgatgcaggg catggccatc gattcgtccg gtgcgttctg gttctcccgg
     4561 ctggctcccg ccaactacga gtcgatcgaa gactgggttc ggcaactcgt cagtgcctca
     4621 cctaccttga cgcaggccga ggtcgtcggt gacttccgat cggcatcagg cagggtcggc
     4681 gtgatcgcga tgctgtcgct gcccgagcag gccgactgtg ggttcgagct cccgaccctt
     4741 cccttccgcg agcccacccc gacatcgcct gcggcgctcg cggcgaccgg ggcgagtgat
     4801 cccgcgccga ttgtcgttct tgcgatcggc accgcagtgg ccggtgctgc gttgattggt
     4861 gcagcgcgtc tgcgctcgcg tcgtcgctag gagttctcga ccggcgcgct gggcagccgc
     4921 gcaccgcccg ggataagccc catggccttc accagagttt cagcaccccc gaaggctacg
     4981 gcgtaacagt tccagccgat ccctgctgcg ctgacgagtt cgaagcgggc cgagaagctc
     5041 acggcctccg gcgcgaagaa gcccgtgtag gcagcgcacg ttgtctgagc ggtgcgattg
     5101 gcggtgccca ctgtcacgag gatgcccggc agaaccaatc ccacaagacc gagcacaacc
     5161 acgacacgca tcaccgtcat caggtgcgga ctgcgcagag gcttctcatg cggctggtat
     5221 cccgcgagtt ccggctcgtc gtcgtactga gagtcatcgt gcatggcgcg gcccattctg
     5281 ccacccccgc ccgagactcg tcccgatgct cccgacatct ccgttgtcag cccggtggtg
     5341 tcatcgcccg agccatcccc cgtccacggg cagcacgatg cccgagacat agtccgacgc
     5401 ggcagaggcg aggaataccg ttgcgccgcc gaggtctgca ccgacgcccc atcgcccggc
     5461 cgggatacgg tcgaggatcg cccgggagcg ggccgggtca tcctgaagcg cctgcgtgtt
     5521 gtcggtggcg atgtaacccg gggcgatcgc gttgacgttg accccgcgcc ccgcccactc
     5581 gttcgccaga gccttggtca atccggccac gcccgacttg gccgcggtgt agccgggcac
     5641 attcacaccg ccctgaaagc tcaacatcga ggccgtgaag atgatcttgc cgtggccgcg
     5701 ctcgagcatc gatcgtccca ccgcctggct cagcacgaac tggctcgaca ggttcacctg
     5761 gatcacgcga tcccacagct cgatcgggtg ttcggccgcc ggtgctcgct cgatggtgcc
     5821 cgcgttgttg acgaggatgt cgacgggccg taccgcgagt cgctcaccga gcgctgcaac
     5881 cgcgtcgacg tcggcgaagt cgcaggcgat ggcctcgaag cgcctgccgt gggaggtgac
     5941 ggcctgctca atggcactgc cggatgcctc gagagttgca ctcacgccga tgatgtcggc
     6001 gcccgcctgc gcgagcgcct cggccatcgc gaatccgatg ccgcgccgtg cgccggtgac
     6061 gatggccagc cgtccggtga ggtcgaacgc gctcgtcact tctggcccgc cacatccacg
     6121 aggatcttca tcgcgcgccc tgcttcgagg tcggcgaagg ccgcctgggt ttcgctcagc
     6181 ggcacgatcc tggtgatcag caggtcggcg gggatcgcgc cgcgctcgag cagctcggcc
     6241 gcggtctcga agtcctgacg ttggtagacc cgggcaccca ggatgctcag ctcgcgccag
     6301 aacacccgct gcaggttgat ctcccgcggg gtggggtgga tcgcgaccac cacgatggtg
     6361 ccccgcacct tggcgaggtc ggtcgctccg agcacggctg cggcggcgcc cgacacctcg
     6421 aacacgacat cggcccccgc gttctgggtc cactcggtga cccaggccac ctgatccacc
     6481 tcgcggggat cgatcgtcgt gaatccgagt tcgctgatct ggccgcgccg gtgcggatcg
     6541 agttcgatga ccgtgacgtc gcccccgaac tcccgggcca ccgtggcgat cagaacaccg
     6601 atcgggccgc cgccgatgac gacaaccttc tcgccggccg cgagattcgc ccgacgcacg
     6661 tcgtggaccg cgacggcgac cggctcgatg agggcggcgt gatcgagtcg caggccctcg
     6721 gggaggcgca cgagtacatc ggtggacacg ttccatagct tctggagaga gccgggcgag
     6781 tcgatcccga tgaagttcag gttctggcag atgtgctggt tgccggcgag acaggccggg
     6841 caggtgccat cccagtccag cggcatgaca gtcacggcgt cacccactgt ccagtcggcg
     6901 acgccctcac cgatcgctgc gatggtgccg ctcatctcgt ggccgaagat caggggagtg
     6961 ctcaccctcg catccatggt gccgtggacg atgtgcaggt ctgttccgca caggccggtg
     7021 taggcgacgg caatctgtac ctcgcccggc ccggggtcgg cggcgtcggc ttcgcgaata
     7081 ctgatggtgt tgtcgcccac gtagttggct gtcagcactg acgcgctcct attcgtaatt
     7141 cgttattcgt atgatgtcta gtatggtcgc atcgg
//
LOCUS       DAOSOU010000096         7122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10556_length_7122_cov_6.871515, whole genome shotgun sequence.
ACCESSION   DAOSOU010000096 DAOSOU010000000
VERSION     DAOSOU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10556_length_7122_cov_6.871515"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLW15_06580"
     CDS             complement(<1..349)
                     /locus_tag="PLW15_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT34094.1"
                     /translation="MSRTPRFARVAAALGAATIALTLAACTPAAETTSEFVTDGKLTI
                     GTGQPAYYPWVIDDAPESGEGFEAAIAYAVADQLGFAKDDVVWVRTTFDEAIAPGPKN
                     FDFNLQQYSITDER"
     gene            449..1819
                     /locus_tag="PLW15_06585"
     CDS             449..1819
                     /locus_tag="PLW15_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34095.1"
                     /translation="MTAIAPVLSRVASVARTVASSSMAVWAAFVLVHLWLGLLNLWAP
                     GLPLGDVTIVYKFWMDQALIAQYWVGIDTVWVYPIVALVPMLAAYAFGPEQYAATWLS
                     MIMVLNAVAFGFLTGWGRSRERVAVAWWWVAFLFLLGPIALGRIDSVTVPLAIIGVLL
                     IARSPVVATVVLTVATWIKVWPAALIAAAVIALRGRLRIVATALLASALIMIVALTLG
                     SGANVLSFVTQQTGRGLQIEAPVSTFWLWQAAAGVPGAFVYYDQQILTFQINGDGVDV
                     ASALMTPLLGLAILVIALLGVRATRSGVAAGDLLPSLSLALVTALIAFNKVGSPQFVS
                     WLAVPVVLGLATRAAGHGRSFRTPAIMVLVIAGLTQFIYPYLYTALLQVQVPMLIVLT
                     GRNAMLFVLLGWAIWSIATAPRLPLLDVDADGTHDDWLDSVWPLDRDLPGEEHSSVIM
                     PAALKE"
     gene            1823..2137
                     /locus_tag="PLW15_06590"
     CDS             1823..2137
                     /locus_tag="PLW15_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOT34096.1"
                     /translation="MLVAFSVAPSGGGDGSDSVHDAVAAAVRVVRESGLPNHTDSMFT
                     TIEGEWDEVFAVIKRATDEVGRFGTRVSLVLKADIRPGHTGELTGKVERLEGAIREAA
                     LD"
     gene            2213..3472
                     /locus_tag="PLW15_06595"
     CDS             2213..3472
                     /locus_tag="PLW15_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34097.1"
                     /translation="MTHSPARIRLALLALALGGFGIGLTEFVAMGLLPNLAADLLPGV
                     WAQSHEQANAQAGWIVSAYALGVVVGAPTIAAAAARFPRKQLLLACVAVFVFGTVASA
                     LLPTFGLVLVARFVAALPHGAYFGIAALVAASLMGPGKRGRGVALVLSGLTIANVIGV
                     PTVTWLGQVAGWRVAYLAVAAVFLLTFAAIALAVPLQPGDPAATIRRELKIFGRVQVW
                     LALLIGAVGFGGFFAVYTYVSPVVTTVTQLSPAVVPWALVAAGIGMTIGNFVGGRAAD
                     HNVMATLFICFGVFAVSLTGLALTAQTPVGLFVFLFLVGAAASALSPAIQTRLMDVAG
                     DGQSLAAAINHSSLNIGNSLGAYLGGLTIAAGLGYLSPTWVGLLLCVPGVALALVALV
                     LQRRSDRSEAARQIRLDEDAVLDRTLA"
     gene            complement(3410..4078)
                     /locus_tag="PLW15_06600"
     CDS             complement(3410..4078)
                     /locus_tag="PLW15_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT34098.1"
                     /translation="MTEMNRIRLAIVDDHRMLLGALTEWIRNAASDIDMVAAVPTWPE
                     LTIHPEFPVDVVLLDVDLKDNIPISLKINTLKTMGVKVVLMSTYSEPNLVREALAAGA
                     LGYLVKSEDAGMIVEAIRAASKGEPFVSAELDLALNAGEIGGAPKLSAQERRVMALYG
                     GGEPVKAVAFQLSISEETAKSYLKRIREKYRVAGFDVGTKVALRKRAIEDGILVESDL
                     PRGF"
     gene            complement(4119..5369)
                     /locus_tag="PLW15_06605"
     CDS             complement(4119..5369)
                     /locus_tag="PLW15_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34099.1"
                     /translation="MTSLPEENTLGAIVGAKSQRREADATRRVIRAATPRRSSLGTGF
                     IGIGAAVVVALRAIYGFVWFVSLWPTYPDPMPALLAWVILIATLVATFITARVIGDQL
                     PDWAFVLFLAGLAAAVALDLVAVWPLHDIGRNATAALTAGMALLVVITLRRTRDILIA
                     VSVLGVGLAVAMVLNTRPDPDDIAPQITALAFAVLPAVIGVVVVNGFRRLVQVELDRV
                     LVQSTVSAPRFAVGMMASEELARLDLAAEELLESVATGKTKLPLDPKTASVAASLATE
                     LRLHLIEGRRETWLYHAVTESDLLGKAVTLIDKGSLAGLLDPQQRDGLLSTAWLLVSD
                     TAKRTSDRTVQLQIGPVSSPAQEGTGHKILVPIVITTTGVARNRVDPATWEAIGRVGQ
                     YSDSTQNSSLRVDISCLVDNPADQ"
     gene            complement(5366..6529)
                     /locus_tag="PLW15_06610"
     CDS             complement(5366..6529)
                     /locus_tag="PLW15_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT34100.1"
                     /translation="MDLIAKERDRLLQRTARIYGLSFTIVSLICLSIPGSVNAVAYFI
                     ALPLYAIVAVSQFRLGVSRSLLWIGPGILAGIAAIVVIAIVTPGGPGAAGVSAAMQLA
                     AASLASFAIVLTTDTVRRMLLAGAFLVVGSLTALLLEPVHTPLRSVPLLVLGWTLATI
                     IGFWISAGVPQAARRIASIGRAHRAERQASETEAQRRQGARLLHDTVLATLTLLAHSG
                     VGVAPESLRQQAADDARLLRQLRLGATPTPQSSGVYNLEGVEDTVLGTTLESVKQRFG
                     RMGLEVSWHGTGQVLLPSDVLDAFLLALAECLENVRRHSGVTEAHVTITDDETTVRAM
                     ITDSGVGFTLDDVDSARLGFKESVVGRLKEVGGRARLFSAPGSGTTVVLEVPR"
     gene            complement(6808..>7122)
                     /locus_tag="PLW15_06615"
     CDS             complement(6808..>7122)
                     /locus_tag="PLW15_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34101.1"
                     /translation="ADGTLTIVESKATVLLGLVDEAGNAVYEHAEQIVVDAGSGPGVV
                     RLRLVPARCDPHAVAEDKRGTFFPLEVKTSTGRSGQLYVPVSDAVRASLYDFFGDYCG
                     LP"
BASE COUNT         1063 a         2373 c         2441 g         1245 t
ORIGIN      
        1 tgcgctcgtc ggtgatcgag tactgctgaa ggttgaagtc gaagttcttc gggccgggag
       61 caatggcctc gtcgaaggtg gtgcgcaccc acaccacgtc gtccttggcg aagccgagct
      121 ggtcagcgac cgcataggcg atggcggcct cgaagccctc tccggactcc ggcgcatcgt
      181 cgatgaccca cgggtagtac gcgggctggc cggtgccgat ggtcagcttg ccgtcggtca
      241 cgaactcgct cgtggtctcg gcggcgggcg tgcacgcggc gagggtgagg gcgatggtgg
      301 cggcaccgag ggcggcggcc acccgggcga aacggggagt gcgggacatg gtcgttctcc
      361 ttggctgcag tgaagggatt tccatcttgc cccaggttgg tcaaccgcac ggaacgggga
      421 cgaaatgtta ccgggctacg ctggtggcat gaccgcgatc gcgcccgtgc tgtcgcgcgt
      481 cgcatccgtc gcccgcactg tggcgtcgag ttcgatggcg gtctgggcgg ccttcgttct
      541 ggtgcacctc tggctcggac tgctgaacct gtgggcaccc ggtctgccgc tgggcgatgt
      601 gacgatcgtc tacaagttct ggatggatca ggcgctcatc gcccagtact gggtgggcat
      661 tgacaccgta tgggtgtacc cgatcgtcgc gctcgtgccg atgctcgccg cctatgcgtt
      721 cgggcccgag cagtacgcgg ccacgtggct gtcgatgatc atggtgctca acgccgtcgc
      781 cttcggcttt ctcaccggat ggggtcggtc gcgtgagcga gtcgccgtcg cctggtggtg
      841 ggtcgccttc ctcttcctgc tcggacccat cgccctcgga cgcatcgact ccgtgacagt
      901 tccgctcgcc atcatcggcg ttctgctgat agcgcgctcg ccggttgtcg caaccgtggt
      961 gctcaccgtc gcaacgtgga tcaaggtgtg gccagcggcc ctgatcgcgg ccgcggtcat
     1021 cgcgctgcgg ggacggctgc ggatcgtcgc caccgcactg ctcgcctccg cgctcatcat
     1081 gatcgtggca ctcacgctcg gcagcggtgc gaacgttctg agcttcgtca cccagcagac
     1141 cggacgcggt ctgcagatcg aggcgcccgt gagcacgttc tggctctggc aggccgccgc
     1201 cggggtgccg ggtgcgttcg tgtactacga ccagcagatt ctcaccttcc agatcaacgg
     1261 agacggtgtc gacgtggcat ccgctctcat gacaccgctg ctggggctcg cgatcctcgt
     1321 catcgcgctg ctgggggtgc gggccacccg tagtggcgtg gccgcgggtg acctgctgcc
     1381 ctcgctgagc cttgctctcg tgacggcgct gatcgccttc aacaaggtcg gctcgccgca
     1441 gttcgtgagc tggttggctg ttcccgtcgt gctcgggctg gccactcgag cggccgggca
     1501 cggtcgttcg ttccggactc cggcgatcat ggtgctcgtg atcgccgggc tcacgcagtt
     1561 catctatccg tacctctaca ccgcgctgct tcaggtgcag gtaccgatgc tgatcgtgct
     1621 gaccggtcgc aacgccatgc tgttcgtgct gctggggtgg gcgatctggt cgattgcgac
     1681 ggcaccgcgc ctgcccttgc tcgacgtcga cgccgacggc actcatgatg actggctcga
     1741 ctcggtctgg cccctcgacc gcgacctgcc gggcgaggaa cactcgtctg tgatcatgcc
     1801 cgccgcactg aaggagtagc ccatgctcgt cgcattctcg gtagcaccca gcggtggtgg
     1861 agacggctcc gactccgtgc acgacgcggt cgcagccgcc gtgcgcgtcg tccgcgagtc
     1921 ggggttgccc aatcacaccg actcgatgtt cacgacgatc gagggggagt gggacgaggt
     1981 cttcgccgtg atcaaacggg caaccgatga ggtgggacga ttcggcacgc gggtgtctct
     2041 cgtgctcaag gccgacatcc gccccggtca caccggcgaa ctcaccggca aggtcgagcg
     2101 cctggaaggc gccatccgcg aggccgcgct cgactgatcg gcactcggcc ggaccctaga
     2161 cttgacgggt ctcgacggtg agcgctcccg tcttctgcgt cgatcggttg aaatgactca
     2221 ctctcctgcc cgtattcgcc tcgctctgct tgcgctcgcg ctcggcggct tcggaatcgg
     2281 gctcacggag ttcgtggcca tggggctgct gcccaacctc gccgccgatc ttcttcccgg
     2341 ggtctgggct cagtcgcatg agcaggcgaa cgcgcaggcg ggatggatcg tctcggcgta
     2401 cgcgctcggc gtggtcgtgg gcgccccgac cattgcagcg gcggcggccc ggttccctcg
     2461 caagcagctg ctgctcgcct gcgttgccgt cttcgtgttc ggcaccgtgg cctccgccct
     2521 gctgccgacc ttcggtctcg tgctcgtggc tcgcttcgtg gcagcactgc cgcacggtgc
     2581 ctacttcggt atcgcggccc tggtggccgc gtcgctcatg gggccgggca agcgcgggcg
     2641 gggcgtggct ctcgtgctct cggggctcac catcgccaac gtcatcgggg tgccgacggt
     2701 gacctggctt ggccaggtcg cgggatggcg ggtcgcctac ctggccgtgg ccgccgtgtt
     2761 cctgctcacc tttgcagcga tcgcgctcgc cgtgccgctg cagccgggcg acccggcggc
     2821 gacgatccgc cgggagctga agatcttcgg tcgcgtgcag gtgtggctcg ccctgctcat
     2881 cggcgccgtc ggcttcggcg ggttcttcgc cgtgtacacc tatgtctccc cggtcgtcac
     2941 gaccgtgacc cagctctcac ccgctgtcgt gccctgggcg ctcgtcgcgg cgggcatcgg
     3001 aatgaccatc ggcaacttcg tcggtggccg ggccgccgat cacaacgtca tggcaacgct
     3061 gttcatctgc ttcggggtct tcgcggtgtc cctcaccggc ctggcgctca cggcccagac
     3121 tccggtcggc ctgttcgtct tcctgttcct cgtgggcgct gccgcatcgg cactgtcgcc
     3181 ggcgattcag accaggctca tggatgtcgc gggcgacggc caatcgctgg ctgcggcgat
     3241 caaccactcg tccctcaaca tcggcaacag cctcggtgcc tacctcggcg ggctcaccat
     3301 cgccgcaggt ctcggctacc tgtcgccgac ctgggtgggg ctcctgctgt gcgtgccggg
     3361 cgtcgccctc gcgctggttg ctctcgtgct gcagcgccgc agcgaccggt cagaagccgc
     3421 gcggcagatc cgactcgacg aggatgccgt cctcgatcgc acgcttgcgt agagcaacct
     3481 tcgtgccgac gtcgaagccg gcgacgcggt acttctcccg gatgcgcttg aggtagctct
     3541 tcgccgtctc ctcggagatc gacagctgga atgccaccgc cttgacgggc tcgccgccgc
     3601 cgtagagcgc catcacccgg cgctcctgcg cgctgagctt cggtgctccg ccgatctcgc
     3661 cggcgttgag ggccaggtcc aactcggcgg agacgaaggg ttcgcccttc gacgccgcac
     3721 ggatcgcctc gacgatcatg ccggcatcct cgctcttgac caggtagccg agcgcaccgg
     3781 cagccagggc ctcgcgcacc aggttcggct ccgagtacgt gctcatcagc acgaccttca
     3841 cgcccatcgt cttcagcgtg ttgatcttga gcgagatggg gatgttgtcc ttgaggtcga
     3901 cgtcgagcag caccacgtca accgggaact ccgggtggat cgtcagttcg ggccacgtgg
     3961 gaacggccgc gaccatgtcg atatcggatg ccgcgttcct gatccactcc gtcagcgcac
     4021 cgagcaacat cctgtggtcg tcaacgatgg cgagcctgat gcggttcatt tcggtcactg
     4081 ggttcccctt ggttcgatct tctggtgctg cggtggtgtt actggtctgc cgggttgtcg
     4141 acgaggcagg agatgtcgac ccgaaggctc gagttctgag ttgagtcact gtactgcccg
     4201 actcggccaa tggcttccca cgtcgccgga tcgacacgat ttcgtgccac gcccgttgtc
     4261 gtgatcacga tgggcacgag gatcttgtgg cccgtgccct cctgagcagg ggaggagacc
     4321 ggtccgatct gcagctgcac agtgcgatcg gaggtgcgct tggcggtgtc gctgaccagg
     4381 agccaggccg tcgacagcag gccgtcgcgc tgctggggat cgaggagccc tgcaagactt
     4441 cccttgtcga tgagcgtcac ggccttgccg agcaggtcgg actcggtgac cgcgtggtac
     4501 agccaggtct cccggcgtcc ttcgatgaga tgcagccgca gctcggtggc gagggatgcc
     4561 gcgaccgacg ccgtcttcgg atccagcgga agcttcgtct tgccggtcgc gaccgactcg
     4621 agcagctcct cggcggcgag gtcgagacgg gccagctcct ccgacgccat catgcccacg
     4681 gcgaaccggg gcgcggagac ggtgctctgc acgagcaccc ggtcgagttc gacctgcacc
     4741 agacgacgga agccgttgac gacgaccacg ccgatgaccg cgggcagcac agcgaacgcg
     4801 agggccgtga tctggggcgc gatgtcatcc ggatcggggc gagtgttcag caccatggcg
     4861 acagcgaggc cgacgcccag caccgatacc gcgatcagga tgtcgcgggt gcgtcgcagg
     4921 gtgatcacga cgagcagcgc catcccggcc gtcaacgctg ctgtggcgtt gcggccgatg
     4981 tcgtgaagcg gccagaccgc gacgaggtcg agagcgacgg cagcggcgag acccgcgagg
     5041 aacagcacga acgcccagtc gggcagctgg tcgccgatga cgcgcgcggt gatgaacgtc
     5101 gcgacgaggg tcgcgatcag gatgacccat gccagcaggg ccggcatcgg gtcggggtag
     5161 gtgggccaga ggctgacaaa ccacacgaag ccgtaaatcg cgcggagggc gacgaccacg
     5221 gccgccccga tgccgatgaa cccggttccg aggctcgagc ggcgcggggt ggccgcgcgg
     5281 atgacccggc gggtggcatc ggcctcgcgt cgctgtgact tggcgccgac gatggcgccg
     5341 agggtgttct cctcgggaag gctcgtcatc ggggcacctc cagaacgaca gtcgttcccg
     5401 agccgggcgc ggagaacagg cgagcacgcc cgccgacctc cttgaggcgt ccgaccactg
     5461 actccttgaa gcccagccgg gccgagtcga cgtcatcgag cgtgaaaccc accccggagt
     5521 cggtgatcat cgctcgaacc gtcgtctcgt cgtcggtgat ggtcacgtgc gcctcggtga
     5581 caccggagtg acgccgcacg ttctccaggc actcggccag cgcgagcagg aacgcatcga
     5641 gcacgtcact gggcagcaga acctgacccg tgccgtgcca actcacctcg agtcccatgc
     5701 gcccgaagcg ctgcttgacc gactcgagcg tggtgccgag caccgtgtcc tcgacgccct
     5761 cgaggttgta gacaccggat gactgcggcg tcggtgtcgc gccgagccga agctgccgca
     5821 gcagacgtgc atcgtcggcg gcctgctggc gcagcgactc gggcgcgacg ccgacaccgg
     5881 agtgtgcgag aagggtgagt gtggccagca ccgtgtcgtg cagcaggcgc gcgccctgtc
     5941 gccgctgcgc ctcggtctca ctcgcctggc gctcggcgcg gtgggcgcgt ccgatgctgg
     6001 cgattcgccg ggctgcctgg ggcacgcccg cgctgatcca gaatccgatg attgtcgcga
     6061 gagtccagcc cagcacgagc agcggcacag atcgaagggg tgtgtgcacg ggttcgagga
     6121 gcagagccgt aagggaaccc acgacgagga atgccccggc cagcagcatc cgtctgacgg
     6181 tgtccgtggt gagcacgatg gcgaacgagg cgagggatgc cgcggccagc tgcatcgcgg
     6241 ccgacacccc cgcggcaccg ggcccgccgg gcgtgacgat cgcgatcacg acgatcgcgg
     6301 cgatgcccgc gaggatcccc ggaccgatcc acagcagcga cctgctcacc cccagacgga
     6361 actggctgac ggccacgatc gcgtacagag ggagggcgat gaagtaggcg accgcgttga
     6421 cggagccggg gatgctcaga cagatgaggg agacaatggt gaagctcagg ccgtagatgc
     6481 gcgcggtgcg ctggagcagc cggtcccgct ccttggcgat gagatccatg tgaagtcatg
     6541 tttccacacc gtgccccaca attgggggtg gctcaccgca ccgcgcggcg gaattctgcg
     6601 gggtacagtc atagtcagag gggcatgcga ggcgactcgc agtcacacaa cgggaaccac
     6661 ccgctcgtgc ccagagcgat ggttccgagc acgtacaacc cgaatgcacg cgccgggtgg
     6721 agcacacagc tccaccaaac cggggggttg ccaggggccg cagcaccaac tgcggcccca
     6781 cccgcagtta acgggcctgt cgagggctca cggaaggccg cagtagtcgc cgaagaagtc
     6841 gtagaggctc gctcgcacgg catcgctcac gggcacgtac agttgccccg accggcccgt
     6901 gctggtcttc acctcgaggg ggaagaacgt tccgcgcttg tcctcggcca ccgcgtgcgg
     6961 atcgcatcgc gcaggcacga gtcgtaaccg cacgacgccc ggcccggagc ccgcgtcaac
     7021 gacgatctgc tccgcatgct cgtacacggc gttgccggct tcgtcgacaa gcccgaggag
     7081 cactgtcgcc ttcgactcga cgatggtgag ggtgccgtcg gc
//
LOCUS       DAOSOU010000097         7121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10558_length_7121_cov_5.789273, whole genome shotgun sequence.
ACCESSION   DAOSOU010000097 DAOSOU010000000
VERSION     DAOSOU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10558_length_7121_cov_5.789273"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PLW15_06620"
     CDS             complement(<1..157)
                     /locus_tag="PLW15_06620"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT34102.1"
                     /translation="MPVATPDQYAEMLDKAKSRGFAYPAINVSSSSTINAVLQGLTEA
                     GSDGIIQV"
     gene            288..1280
                     /gene="glpX"
                     /locus_tag="PLW15_06625"
     CDS             288..1280
                     /gene="glpX"
                     /locus_tag="PLW15_06625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOT34103.1"
                     /translation="MSTSETGTLFLHPDRNLALELVRATEAAAIRAVPFIGKGDKLGA
                     DGAAVDAMRKFLGTVDFDGLVVIGEGEKDNAPMLFNGEHVGTGRGPACDIAVDPIDGT
                     SLTAAGRQNAISMIAVSDRGTMLDASSVFYMNKIVTGAEGRGVIDIRQPIGENIRAFA
                     KAKGKPVSEIVVAVLDRPRHEGLIEEIRATGAGTRLMLDGDVAGGINAALYGTRIDMC
                     AGIGGSPEGVATACAIKALGGVIQTILAPKDDEERARGEAAGLLFDHVYDADSLVRSD
                     NTFFVATGVTDGGLVRGVRRLGPIIRTESIVLRSHSGTIRRVEADHLAAKWLDD"
     gene            complement(1373..2734)
                     /locus_tag="PLW15_06630"
     CDS             complement(1373..2734)
                     /locus_tag="PLW15_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT34104.1"
                     /translation="MDPILALIVGLAAGAVLGAAIGLLAGRLRGAGADPAVIEARHAT
                     ALAQLQADEQERRAELGSQLAAAQASLAGLREQLHMAQQQYGEAVERAQAEAQERAAE
                     GKVLQALSPVQETLRTMQQKVLDMEQQRSRQHGELSEQLRTATESEERLRATAESLAS
                     ALRNNATRGVWGETQLRNLVESAGLLNRVDFTVQTSISSESGARRPDMVLRLPGGKSI
                     AVDAKVPYNSYIDASAIPATATGEQEARRQSLLAQHAKQVRSHVDALSAKSYWTGLDS
                     SPEFTMAFIPNESLLSVALDQDPGLLDYAFSKRVLLASPVSFWATLKTVAFTWQQEVL
                     TEDAKRLFDLGKELFGRIATLGEHAEKLRRSLDSTVANYNQFASSLESRVLVTARKLD
                     QLDESKIIPAPGVVEERPKPLSAIEFELASLDELEGVARPELELPGMELPAVPAKGSK
                     KTA"
     gene            2819..3739
                     /locus_tag="PLW15_06635"
     CDS             2819..3739
                     /locus_tag="PLW15_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT34105.1"
                     /translation="MTASVPHPLDVSTDDLRYLLAVARSGRLVSAATLLGVDHTTVRR
                     RIDRLELALGVRLLDRGADGWELTVIGRDVAARAAGLEHVVEQVVDAASGGADGPRGT
                     VRIVAPEGFGTVFAAPCLVRVQAEHPGITVELVTSTSPLSLRGSGFDIAVTVGSATSS
                     RLTAEALAPYALRLYASRDYLAAHPPIVSLSDLERHPLVFYVDALLTVRELDLAPVLN
                     GMHVGFGSTNVFAQLEATRSGAGIGLLHAFMAESDPRLAPVLPDEVDFRLMFSLSVRK
                     DSLGVDAVRVVRDALFAEVRQRADELLPVR"
     gene            3835..5328
                     /locus_tag="PLW15_06640"
     CDS             3835..5328
                     /locus_tag="PLW15_06640"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOT34106.1"
                     /translation="MTEVLDHWINGAAAAGASTRTAPVYNPAEGTVSREVRLATVADV
                     DVAVQAAKAAFPAWADSSLAKRQTVLFAFRELLNARKSEVAAILTNEHGKVTSDALGE
                     VARGLEVVEFATSIPHLMKGEYSENVSTGVDVYSLHQPLGVVGIISPFNFPAMVPLWF
                     FPIAIAAGNTVVLKPSEKDPSAAIWMAKLLKEAGLPDGVFNVVHGDKESVDALLEHPD
                     VQAISFVGSTPIAKYIYETASHHGKRVQALGGAKNHMLVLPDADLDLVADSAVNAGFG
                     SAGERCMAISVVVAVEPVADELIAKIQERMSGLKVGDGTRGCDMGPLITKEHRDKVAG
                     YIDIATKDGATVVVDGRGIQADGGKDGFWLGPTLIDNVSTDSDVYKHEIFGPVLSVVR
                     VATYDEGLALINSSQYGNGTAIFTNDGGAARRFQREVTVGMVGINVPIPVPVGYFSFG
                     GWKDSLFGDTKAYGPDAIHFFTRQKAVTSRWLDPSHGGINLGFPQNG"
     gene            complement(5393..5977)
                     /locus_tag="PLW15_06645"
     CDS             complement(5393..5977)
                     /locus_tag="PLW15_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOT34107.1"
                     /translation="MPLDFTAIDFETANNSPASACSVGLVKVRGGRVVDSAYWLIRPP
                     FGHDAFLEWNVRIHGIMAADVTDALLWSEQLHDLQSFAENDVLVAHNAGFDMGVIAAA
                     SAASFVPTPTYDYLCSLQVARRTYHLDSYKLPSAAMAAGFEDFAHHNALADAEACAAI
                     IVHAADRHGASDIAELATAVGAQLKRVGALQPVE"
     gene            6074..>7121
                     /gene="ychF"
                     /locus_tag="PLW15_06650"
     CDS             6074..>7121
                     /gene="ychF"
                     /locus_tag="PLW15_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT34108.1"
                     /translation="MALTIAIVGLPNVGKSTLFNALTKNSVLAANYPFATIEPNVGVV
                     NLPDPRLKVLAGIFGSERILPAPVSFVDIAGIVKGASIGEGLGNKFLANIREADAIAQ
                     VIRGFTDADVVHVDGRVDAASDMETINTELILADLQTLTKAIERYEKEVKTKRAEPVV
                     LETALAAQKVLDSGKPLSASGLDLEPIRELGLLTAKPFIYVFNVDEAILTDAAKKAAL
                     AALVAPAHAVFLDAKLESELIDLDEADAAELLASTGQEESGLDQLARIGFDTLGLQTY
                     LTAGPKESRAWTIGKGWKAPQAAGVIHTDFEKGFIKAEVISFEDLVECGSVLEARHHG
                     KARLEGKDYVMKDGD"
BASE COUNT         1147 a         2374 c         2426 g         1174 t
ORIGIN      
        1 tcacctggat gatgccatcc gaacccgcct cggtgaggcc ctggagcacc gcattgatcg
       61 tcgacgagga tgacacgttg atcgcggggt acgcgaagcc gcggctcttg gccttgtcga
      121 gcatttctgc gtactggtct ggcgtggcga caggcatcga tggtctcctt ggaatgcggg
      181 tggtgtccga aacaaaagtc tagcgagggc gaacgctagg ctggacgggc gttggttcgc
      241 cgattgaact ccaccacagc gttcggctct ttcacgggat gttttccatg tcgacctctg
      301 aaaccggaac gctgttcctc caccccgatc gcaacctcgc cctcgagttg gtgcgagcga
      361 ctgaagccgc ggccatccgt gccgtgccgt tcatcggcaa gggcgacaag ctcggcgccg
      421 acggtgcggc cgtggatgcc atgcgcaagt tcctcggcac cgtcgacttc gacgggctcg
      481 tggtgatcgg cgagggcgag aaggacaatg cgccgatgct gttcaacggc gagcatgtgg
      541 gtaccggacg cggtccggcg tgcgatatcg ccgtcgaccc gatcgacgga acctcgctga
      601 ccgctgccgg acgtcagaac gcgatctcga tgatcgccgt atccgaccgg ggcaccatgc
      661 ttgacgcgtc gagcgtgttc tacatgaaca agatcgtcac cggcgcagaa ggccgggggg
      721 tcatcgacat tcgccagccc atcggcgaga acattcgcgc gttcgcgaag gcgaagggca
      781 agccggtgag cgagatcgtc gtcgccgtgc tggaccgtcc ccggcacgag ggactcattg
      841 aggagattcg cgccaccggg gcgggcactc gcctcatgct cgacggcgat gtggccggcg
      901 gaatcaacgc ggcgctctac ggcacgcgca tcgacatgtg cgcgggtatc ggcggcagcc
      961 ccgagggtgt ggcgacggcg tgcgcgatca aggccctggg cggcgtcatc cagacgatcc
     1021 tcgccccgaa ggacgacgag gagcgcgcgc ggggagaagc ggcgggcctg ctcttcgatc
     1081 acgtctacga cgctgacagc ctggtgcgca gtgacaacac gttcttcgtt gcgacgggcg
     1141 tcaccgatgg cgggctcgtt cgcggtgtgc gtcggctcgg gccgatcatc cgcaccgaga
     1201 gcattgtgct gcggtcgcac tcgggcacga tccgccgggt cgaggccgac cacctcgccg
     1261 cgaagtggtt ggacgactag ccggcctcac cggcctgccc tcgtcagtga catcgaggtg
     1321 gccgaaaagc tggatgcgaa ggggctggca cccagcattg tggctacctc gactaggccg
     1381 tcttcttgct gcccttggcg ggcacggccg gcagctccat gccgggaagc tccagctcgg
     1441 ggcgggcgac tccctcgagc tcatcgaggg aggctagctc gaactcgatg gcgctgaggg
     1501 gcttgggccg ttcttccact acgccggggg cgggaatgat cttggattcg tcgagctggt
     1561 cgagcttgcg ggcggtcacc agtacgcggg actccaggga gctcgcgaac tggttgtagt
     1621 tggccacggt cgagtcgagg gagcgacgca gcttctcggc gtgctccccg agggtggcga
     1681 tgcgaccgaa gagctccttg ccgaggtcga acagccgctt ggcgtcctcg gtgagcacct
     1741 cctgctgcca agtgaacgcg acggtcttca gagtcgccca gaagctcacg ggggaggcga
     1801 gcagcacccg cttgctgaag gcgtagtcga ggaggccggg atcctgatcc agcgccaccg
     1861 acaggagcga ctcattggga atgaaggcca tggtgaactc ggggctcgag tcgaggccgg
     1921 tccagtagct cttggccgac aacgcgtcga cgtgcgagcg cacctgcttc gcgtgctgcg
     1981 cgagcaggct ctggcgccgc gcctcctgct ctccggtggc agtcgcgggg atggcgctcg
     2041 cgtcgatgta ggagttgtag ggaaccttcg cgtcgaccgc gatcgacttg ccgccgggaa
     2101 gccgcagcac catgtcgggg cgacgcgcgc cggactcgga gctgatgctg gtctgcacgg
     2161 tgaagtcgac gcggttgagc aggccggccg attcgaccag gttgcgcaac tgggtctcgc
     2221 cccagacgcc acgggtcgca ttgttgcgca gggcggatgc gagcgattcc gcggtcgcac
     2281 gcagtcgctc ctccgactcg gtcgcggtgc gcaactgctc ggagagctcg ccgtgctggc
     2341 ggctgcgctg ctgctccatg tcgagcacct tctgctgcat ggtgcgcagc gtctcctgca
     2401 ccggagacag cgcctgaaga accttccctt cggccgcgcg ctcctgcgcc tcagcctggg
     2461 cacgctcgac ggcctccccg tactgctgct gcgccatgtg cagttgctcg cggagtcccg
     2521 cgaggctggc ctgcgcggca gcgagctgcg aaccgagctc ggcccgccgc tcctgttcgt
     2581 ctgcctgcag ctgtgcgagc gcggtcgcgt gccgtgcctc gatcacggcg ggatccgcac
     2641 ctgcgccgcg cagtcgcccg gcaagcagac cgatggcagc gccgagcacg gcgccggcgg
     2701 cgagtcccac gatgagagcg aggatggggt ccatgggccc agtatcaccc gggcgtcgga
     2761 cattacccgg cttcgaaccg cgaggcagtg caagccgggc cggtcatagg gtggtgtcat
     2821 gaccgcgtcg gttccgcatc cgctcgatgt gagcaccgac gacctgcgct atctgctcgc
     2881 cgtcgcacgg tcgggcaggc ttgtctccgc cgccacactg ctcggtgtcg atcacaccac
     2941 ggtgcgcaga cgcatcgacc ggttggagtt ggcgctgggt gtgcggctgc tcgaccgcgg
     3001 cgccgacggc tgggagctga ccgtgatcgg tcgggatgtc gcggcacgag ccgccgggct
     3061 cgagcacgtc gtcgagcagg tcgtcgacgc ggcatccggg ggagccgacg ggcctcgggg
     3121 cactgtgcgg atcgtcgcgc ccgagggctt cggaactgtc ttcgcggccc cctgtctggt
     3181 cagggttcag gcggagcatc ccggcatcac tgtcgaactg gtcacctcga cgagcccgct
     3241 gagcctgcgc ggctcgggct tcgacatcgc cgtcacggtc gggtccgcca cgagttcgcg
     3301 gctgaccgcc gaggccctcg cgccctacgc cttgaggctg tacgcctcgc gcgactacct
     3361 cgccgcgcac ccgccgatcg tgtcgctgag cgacctggag cggcatccgc tcgtcttcta
     3421 cgtggatgcc ctgctcaccg tgcgcgaact cgacctcgct cccgtgctca acggcatgca
     3481 cgtgggtttc gggtcgacca acgtgttcgc ccagctggag gccacccgca gtggggcggg
     3541 catcggcctg ctgcacgcct tcatggccga gtcggacccc aggctggcgc ccgtgctgcc
     3601 cgacgaggtc gacttccgtc tcatgttctc gctgtcggtt cgcaaggaca gcctcggagt
     3661 ggacgcggtg cgggtggtgc gtgatgcgct cttcgccgag gtgaggcagc gcgcggacga
     3721 gctgctgcct gtgcgatagt gcacattgcc tgtgcatgaa tagctcttga atgcagatca
     3781 ctcgggctcc agactgggac cacaccacac gaaccgcgac gaagagagcg tgccatgact
     3841 gaagtcctcg accactggat caacggagcg gccgcggccg gcgcctcgac tcgcaccgcg
     3901 cccgtgtaca accccgccga ggggaccgtc tcgcgcgagg tgcgcctggc gaccgttgcc
     3961 gatgtcgacg tcgccgtgca ggccgccaag gccgccttcc ccgcctgggc cgactcctcg
     4021 ctggccaagc gccagaccgt cctcttcgcc ttccgcgagc tgctcaacgc acgcaagtcc
     4081 gaggttgcgg cgatcctcac caacgagcac ggcaaggtca cgagcgacgc tctgggcgag
     4141 gtcgcccgcg ggctcgaggt cgtggagttc gcgacatcca tcccgcacct catgaagggc
     4201 gagtactcgg agaacgtgtc gaccggtgtc gatgtctact cgctgcacca gccgctcggg
     4261 gtcgtgggca tcatcagccc gttcaacttc ccggccatgg tgccgctctg gttcttcccc
     4321 atcgcgatcg ccgcgggcaa caccgtcgtg ctcaagccga gcgagaagga cccgtcggcc
     4381 gccatctgga tggcgaagct gctcaaggag gctggcctgc ccgacggcgt gttcaacgtc
     4441 gtgcacggcg acaaggagtc ggtggatgcg ctgctcgagc accccgacgt gcaggcgatc
     4501 tcgttcgtgg gctccactcc catcgccaag tacatctacg agacggcatc tcaccacggc
     4561 aagcgggtgc aggcgctcgg tggcgcgaag aaccacatgc tcgtgctgcc cgacgccgac
     4621 ctcgacctcg tcgctgactc ggccgtcaac gcgggcttcg gctcggccgg cgagcgctgc
     4681 atggcgatct ccgtcgtggt ggccgtcgag ccggtggccg acgagctcat cgccaagatc
     4741 caggagcgca tgtcgggcct gaaggtcggc gacggaaccc gcggctgcga catgggtccc
     4801 ctcatcacga aggagcaccg cgacaaggtc gcgggctaca tcgacatcgc cacgaaggat
     4861 ggagcgaccg tcgtggtcga cggccgtggc atccaggcgg atggtggcaa ggacggcttc
     4921 tggctcggcc cgacgctcat cgacaacgtc tccaccgact cggacgtcta caagcacgag
     4981 atcttcggac cggtgctgtc cgtcgtgcgc gtcgcgacct acgacgaggg cctcgcgctc
     5041 atcaactcca gccagtacgg caacggcacg gccatcttca ccaacgacgg gggagcggcc
     5101 cgccgcttcc agcgcgaggt caccgtgggc atggtcggca tcaacgtgcc gatcccggtg
     5161 ccggtgggct acttctcgtt cggtggttgg aaggactcgc tgttcggtga cacgaaggcc
     5221 tacgggcccg acgccatcca cttcttcacc cgccagaagg cggtcacgag ccgctggctc
     5281 gaccccagcc acggcggcat caacctgggc ttcccgcaga acggctagcc gctggttgag
     5341 taggcgcgca gcgcggtatc gaaacctgcg aggtctcgat acggccttcg gcctactcga
     5401 ccggctgcag ggcgccgacg cgcttgagct gtgccccaac cgcggtcgcg agttcggcga
     5461 tgtcggatgc tccgtgccgg tcggccgcgt gcacgatgat cgcggcgcac gcctcggcgt
     5521 cggccagcgc gttgtggtgc gcgaagtcct cgaagccggc ggccatcgcg gcggacggca
     5581 gcttgtagga gtcgaggtgg taggtgcgac gcgccacctg caggctgcag aggtagtcgt
     5641 acgtcggagt gggtacgaag gaggcggcgc tcgccgcagc gatgacgccc atgtcgaatc
     5701 ccgcgttgtg ggcgaccagt acgtcgttct cggcgaacga ctgcaggtcg tgcagctgct
     5761 cactccagag cagggcgtcc gtgacatccg ccgccatgat gccgtggatg cgcacgttcc
     5821 actcgaggaa cgcatcgtgg ccgaacggcg gcctgatcag ccagtaggcc gaatcgacaa
     5881 cgcgaccgcc gcgaaccttc accagaccga ccgagcacgc gctcgcgggg gagttgttgg
     5941 cggtctcgaa gtcgattgcg gtgaagtcca gcggcacatg tcgatcgtcg caccggcccc
     6001 cgacttaggc ggctgggcac gccgccgtcg ccgggatcga tagcctctgc ggccgccggt
     6061 acgcttgagc ctcgtggctc tcactatcgc gatcgtcgga ctgcccaatg tcggcaagtc
     6121 caccctgttc aatgcactga cgaagaattc cgtgctggcg gcgaactatc cgttcgcgac
     6181 catcgagccc aacgtcggag tggtgaacct tcccgacccg cgcctgaagg tgctcgcggg
     6241 aatcttcggc agcgagcgca tcctgcccgc gccggtctcg ttcgtcgaca tcgcgggcat
     6301 cgtgaagggc gcgagcatcg gggagggact gggcaacaag ttcctcgcaa acatccgcga
     6361 ggccgacgcg atcgcgcagg tgatccgcgg cttcaccgac gccgatgtcg tgcacgtcga
     6421 cgggcgggtg gatgcggcat ccgatatgga gaccatcaac accgagctga tcctcgccga
     6481 cctccagacg ctcacgaagg cgatcgagcg ctacgagaag gaggtcaaga ccaagcgcgc
     6541 cgaaccggtg gtgctggaga ccgccctcgc cgcgcagaag gtcctggact cgggcaagcc
     6601 gctgagcgca tccggtctcg atctcgagcc gattcgcgag ctcggactgc tcaccgccaa
     6661 gcccttcatc tacgtcttca atgtcgacga ggcgattctc acggatgccg ccaagaaggc
     6721 cgcgctcgca gccctcgtcg cccccgctca cgccgtgttc ctcgacgcca agctcgagtc
     6781 cgaactcatc gatctcgacg aggcggacgc ggcggagcta ctcgcctcca ccggtcagga
     6841 ggagagcggt cttgaccagc tcgcccgcat cggcttcgac acgctgggcc tccagaccta
     6901 cctcaccgca ggccccaagg agtcgcgcgc ctggacgatc ggcaagggct ggaaggcgcc
     6961 gcaggcggcg ggcgtcatcc acaccgactt cgagaagggc ttcatcaagg ccgaggtcat
     7021 ctcgttcgaa gacctcgtcg agtgcggctc ggtgctcgaa gcgcgtcacc acggcaaggc
     7081 gcgcctcgag ggcaaggact acgtcatgaa ggacggcgac g
//
LOCUS       DAOSOU010000098         7039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10739_length_7039_cov_5.559708, whole genome shotgun sequence.
ACCESSION   DAOSOU010000098 DAOSOU010000000
VERSION     DAOSOU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_7039_cov_5.559708"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLW15_06655"
     CDS             <1..621
                     /locus_tag="PLW15_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOT34109.1"
                     /translation="VRSEPWRRLGGVHSIRGARNLAVARELWVARDAYAREIDTAPGR
                     LVPDSALVAAARTLPETKRDLAALKEFTGRASRSQIDRWWAAIQAGMTTDDLPALRVP
                     GETVPPPRVWSDKNPEADKRLKRARAAVTEVGTTLSIPVENLLTPELLRRVAWTPPDP
                     IDAQTIGAALEESGARPWQIDATAQVIAKAFVEAAQSPEEPQDSPS"
     gene            695..1894
                     /locus_tag="PLW15_06660"
     CDS             695..1894
                     /locus_tag="PLW15_06660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT34110.1"
                     /translation="MAERAEVVFVDGVRTPFGRAGEKGMFWNTRADDLVVKAMIGLLE
                     RNPQIPQDRYDDVAIAATTQQGDQGLTLGRTAALLAGLPKSVPGYAIDRMCAGAMTSV
                     TTIAGAIAFGAYDIGIAGGVEHMGRHPMGFGADPNPRFLAEKLVSADALNMGNTAERI
                     HDRFPQLTKERADRFALASQQKAAAAYDNGKIQPDLIPVAIRSEAGWGLATRDEALRP
                     ETTMEGLAGLKTPFRPHGRVTAGNASGLNDGATASILASADAAKEFGLTPKMRMVSFA
                     FAGVEPEIMGIGPVPSTEKALRKAGLSIDDIGLFELNEAFAVQVLSLLDHFGIADDDP
                     RVNQWGGAIAMGHPLASSGVRLMIQLAAQFAERPDVRYGLTAMCIGLGQGGSVIWENP
                     HYTGKKK"
     gene            1897..4035
                     /locus_tag="PLW15_06665"
     CDS             1897..4035
                     /locus_tag="PLW15_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOT34111.1"
                     /translation="MAAVTPAERFAKLAALDPDEVITHSFVRDIPISGGKTLALITLD
                     NGKDHTRPNTLGPNTLLRFAEVLDEQAARAKKGEIHGVAVTGKPFIFAAGADLSKVND
                     IPDYETGKQMAQLGHWSLGKLAELGVPSFVFTNGLALGGGVEVGLNADYRTIDSSAAA
                     FALPEVFLGLIPGWGGAWLLPNLIGIENALEVIISNPLKNNRMLSAQQAFDLGIADAI
                     FPPANFLEDSITWADSVISGKTKVKRKNEPGKVERLAKWDIAVGIAEKTLASRIGRVA
                     HSPYKALELIKAAKNTDKKTGFAAEDDALADLVAGDQFRASIYAFNLVQKRAKRPAGA
                     PDKALARPVTKVGVIGAGLMARQFALLFVRRLQVPVVITDLDQTRVDEGVAYIHKEID
                     ELLGKGRISGDESNRLKALVSGTTDKSDFADCDWVIEAIFEELEAKQAVFAEVEKFIT
                     PEAILATNTSSLSVEQIGAKLKNPERVVGFHFFNPVAVMPLIEVVRTPHTTDETLATA
                     MATAAKLRKNAVITKDTPGFVVNRILAKVLGEAMHAVDTGTPFDVVEESTRPFGLPMT
                     PFELLELVGLKVGAHVLDTHHKAFPDRFFESENLHKLAELGHIFERDAKGKVKGIDKK
                     ALDIVKGGTTPMTAQQLQMRIEDGLADEIHRMLEDKVVEAAEDIDLCLILGAGYPFQM
                     GGATPYLDRSGASERAFGDTFHHPPIRGVK"
     gene            complement(4116..5792)
                     /locus_tag="PLW15_06670"
     CDS             complement(4116..5792)
                     /locus_tag="PLW15_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOT34112.1"
                     /translation="MTAERYTVSDYLLDRLAELGVDRIFGVPGDFTLALLDHVDDDTR
                     VEWVAMANELGAGYAADGYARLRGLGVVCTTFGVGELSAINAVAGAYAEHVPLLHLVG
                     SPSTLVQSAGRATHHSLGDGDFAHTLRMTAEVTVAQSMVPLVGAEAEIDRVLTAIVQS
                     SQPGYLSLPTDVGEAPASRPSSPLAIDPPDSDAASLSAFEAAARALLADAPRPLVLAD
                     ILVDRCHAESQLHELLGSADLDFACLLWGRRVIDESDERFLGTYIGAASEPAVQRAVE
                     SAARLITVGVHFTDLTSGFFTQHLDDAHRVDLLPHTATVAGQTFPGIEMRDGLAVLTR
                     ILGAGHRRHGGPGVAPAVEPVPTHEPLTQADLWHVVTDALRPGDLVFADQGTAFYGLG
                     AHRMPHDVLFIGQPLWASIGYTLPAVLGGALAAPERRPVLVIGDGAAQLSIAELGSII
                     RMRIPGIILVVNNDGYTVERAIHGPGRRYNDIARWRWADLPWAFGASPDAVRSVRVET
                     AAELSGALAAAEGEPGILTFIEAVVDPLDVPPLLTKVAEAAAAANARTSR"
     gene            complement(5789..>7039)
                     /locus_tag="PLW15_06675"
     CDS             complement(5789..>7039)
                     /locus_tag="PLW15_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOT34113.1"
                     /translation="FLSRFSNNEALYSNLDIKYLGPVHGHDLNALVEVLQQAKNYGAP
                     VIVHAITEKGRGYQPALLDTADQFHAVGQIDPETGEPIEVASKPSWTNVFADEIVQLA
                     DRNPRLVGITAAMLRPTGLHKFAEKYPDRVLDVGIAEQHAVTSAAGLAFGGLHPVVAL
                     YATFVNRAFDQVLMDVALHKAGVTFVLDRAGVTGPDGASHHGMWDLAILQVVPNIRIA
                     APRDATRLREELGEAVAVDDAPTVVRFSKGSVGIEYETIRRTDDGVDVLREAPHKDVL
                     IVTVGPMAKIGLQVAERLAAQGIGATVIDPRWVVPVPASVITFAAEHRLVITIEDGVR
                     VGGIGTRIRQDMRAAGVDTAVNELGLPDQFLEHGSREYLLEQVGLTPQAIARDVVAQV
                     LGSKIPVARPLPAELTASERVPEQ"
BASE COUNT         1210 a         2424 c         2347 g         1058 t
ORIGIN      
        1 gtgcgttcgg agccgtggcg acggctcggg ggtgttcact cgatccgggg tgcccgcaac
       61 cttgcggtgg cccgcgagtt gtgggtcgcc cgggatgcct acgcccgcga gatcgacacc
      121 gctccgggcc gtctggtacc cgactccgcc ctggtcgccg ccgcgcgcac gctgccggag
      181 acgaagcggg acctcgccgc gctcaaggag ttcacgggcc gggcgagccg gtcgcagatc
      241 gatcgctggt gggcggcgat ccaggcaggc atgaccaccg atgaccttcc cgcgctgcgc
      301 gtgcccgggg agacggttcc gccacccaga gtctggtcgg acaagaaccc cgaggccgac
      361 aagcgactca agcgtgcccg cgccgcggtc accgaggtgg ggacgacgct gtccatcccc
      421 gtcgagaatc tgctcacacc cgagctgctg cgacgcgtcg cgtggacgcc ccccgacccc
      481 atcgacgccc agacgatcgg cgccgccctc gaggaatccg gtgctcgccc ctggcagatt
      541 gacgcaacgg cacaggtgat tgcgaaagcc tttgtagagg cggcccaatc ccccgaggag
      601 cctcaggact ccccttcgta ggaacagtca aaggattcac ggccgttttt tgccgcgcct
      661 aggatcgaga acgaacctag aaaatggagg cactgtggcc gagagagctg aagtcgtgtt
      721 cgtcgatggc gtgcgaaccc cgttcggtcg ggccggcgag aaggggatgt tctggaacac
      781 ccgcgccgat gatctcgtcg tgaaggcgat gatcgggctc ctcgagcgca acccgcagat
      841 tcctcaggac cgctatgacg atgtcgccat cgctgcgacc acccagcagg gcgaccaggg
      901 actgaccctc ggccgcacgg cggcgctcct cgccggtctg cccaagtccg ttcccggtta
      961 cgcgatcgac cggatgtgcg cgggagccat gacctccgtg acgaccatcg cgggcgcaat
     1021 cgcgttcggc gcctacgaca tcggcatcgc cggtggagtc gagcacatgg gtcgccaccc
     1081 gatgggcttc ggagccgacc cgaacccccg cttcctcgcc gagaagctcg tgagcgccga
     1141 cgcgctgaac atgggcaaca cggccgagcg catccacgac cgcttcccgc aactcacgaa
     1201 ggagcgcgcc gaccgtttcg ccctggccag ccagcagaag gcagcggccg cgtacgacaa
     1261 cggcaagatc cagcccgacc tcattccggt cgcgatccgg agcgaagctg ggtgggggct
     1321 cgccacccgt gacgaggccc tgcgccccga gaccacgatg gaggggctcg caggcctgaa
     1381 gacgcccttc cgcccgcacg gtcgcgtcac cgcgggcaac gcgtccggcc tcaacgatgg
     1441 cgcgacggcg agcatcctcg ccagtgcgga tgccgccaag gagttcggcc tgaccccgaa
     1501 gatgcgcatg gtgagtttcg ctttcgccgg ggtggaaccc gagatcatgg gcatcggtcc
     1561 ggttccctcg accgagaagg ccctgcgcaa ggcgggcctg tcgatcgacg acatcggcct
     1621 gttcgaactc aacgaggcgt ttgccgtgca ggtgctctca ctactcgacc acttcggcat
     1681 cgccgacgac gacccccggg tgaaccagtg gggcggcgcg atcgcgatgg ggcaccccct
     1741 cgcgtcatcc ggtgtgcgac tcatgatcca gctcgcggcc cagttcgccg agcgtcccga
     1801 cgtgcgctac ggcctcacgg ccatgtgcat cggcctcggc cagggtggct cggtcatctg
     1861 ggagaaccct cactacaccg gaaagaagaa gtagtcatgg cggcagtcac tcccgcggaa
     1921 cgcttcgcga agctcgcggc tctcgacccg gacgaggtca tcacgcactc gttcgtgcgc
     1981 gatatcccga tctcgggcgg caagaccctc gccctcatca cgctcgacaa cggcaaggac
     2041 cacacccggc ccaacacgct gggcccgaac accctgctgc gtttcgccga ggttctcgac
     2101 gagcaggcgg cgcgcgcgaa gaagggcgag atccacggcg tcgccgtcac gggcaagccg
     2161 ttcatcttcg cggccggggc cgatctctcg aaggtcaacg acatcccgga ctatgaaacc
     2221 ggcaagcaga tggcccagct ggggcactgg tcgctgggca agctcgccga gctcggcgtg
     2281 ccctcgttcg tcttcacgaa cgggctcgcg ctcggcggtg gcgtggaggt cggcctcaac
     2341 gccgactacc gcacgatcga cagctccgcg gcggcgttcg cgctgcccga ggtgttcctc
     2401 ggactgatcc cgggttgggg cggcgcgtgg ctgctgccca acctgatcgg tatcgagaac
     2461 gccctcgagg tcatcatctc gaacccgctc aagaacaacc ggatgctcag cgcccagcag
     2521 gccttcgacc tcggcattgc cgacgcgatc ttcccgccag ccaacttcct cgaggattcg
     2581 atcacgtggg cagactccgt gatctcgggc aagaccaagg tcaagcgcaa gaacgagccg
     2641 ggcaaggtcg aacggctcgc gaagtgggat atcgccgtcg gcatcgcgga gaagaccctc
     2701 gcgagccgca tcggacgcgt cgcccactcc ccctacaagg ccctcgagct catcaaggcc
     2761 gccaagaaca ccgacaagaa gacgggcttc gccgccgagg atgacgccct ggccgacctc
     2821 gtggcgggtg accagttccg cgccagcatc tacgccttca acctggtgca gaagcgcgcc
     2881 aagcgtcctg ccggggctcc cgacaaggct ctcgcgcgcc cggtgaccaa ggtcggcgtg
     2941 atcggcgcgg gcctcatggc ccgccagttc gcgctgctct tcgtgcgccg cctgcaggtg
     3001 ccggtggtca tcaccgacct cgatcagacg cgcgtcgacg agggcgtcgc ctacatccac
     3061 aaggagatcg acgagctgct cggcaagggt cgtatctccg gcgacgaatc caaccgcctc
     3121 aaggcactcg tcagtggaac caccgacaag tccgacttcg ccgactgcga ctgggtcatc
     3181 gaggccatct tcgaggagct cgaggccaag caggccgtct tcgcggaggt ggagaagttc
     3241 atcacgcccg aggcgatcct cgcgaccaac acgtcgtcgc tgtcggtcga gcagatcggc
     3301 gcgaagttga agaaccccga gcgggtggtc ggattccact tcttcaaccc ggtcgccgtc
     3361 atgcctctca tcgaggtcgt gcgcaccccg cacaccaccg atgagaccct ggccacagcg
     3421 atggcgacgg cggcaaagct gcgcaagaac gcggtcatca cgaaggacac ccccgggttc
     3481 gtcgtcaacc gcatcctcgc caaggtgctg ggcgaggcca tgcacgccgt cgacaccggc
     3541 acgccgttcg acgtcgtcga ggagtcgacg cggcccttcg gtctgccgat gaccccgttc
     3601 gagctgctcg aacttgtggg tctcaaggtc ggcgcgcacg tgctcgacac ccaccacaag
     3661 gcgttccccg atcgcttctt cgagagcgag aacctgcaca agctcgccga gttgggtcac
     3721 atcttcgagc gcgacgccaa gggcaaggtg aagggcatcg acaagaaggc gctcgacatc
     3781 gtcaagggcg gcacgacccc catgaccgcc cagcagctcc agatgcggat cgaggatggc
     3841 ctggccgacg agatccaccg catgctggag gacaaggtcg tcgaggctgc agaggacatc
     3901 gacctctgcc tcatcctcgg cgcgggctac ccgttccaga tgggtggtgc aacgccgtac
     3961 ctcgatcgct cgggagcgtc cgagcgcgcc ttcggcgaca ccttccacca cccgcccatc
     4021 cgcggggtga agtaggacca ccgctggttg agtagcgtca gaggcgcgta tcgaaaccgc
     4081 acgagggatt tcgatacgcg cttcgcgcta ctcaatcagc gcgaggtacg agcgttcgcc
     4141 gctgctgcgg cttcggccac cttcgtgagc agcggtggca cgtcgagcgg gtcgacgacc
     4201 gcctcgatga aggtcaggat gcccggctca ccctcggccg ccgccagcgc acccgacagc
     4261 tcagccgccg tctccacccg caccgaccgc acggcgtcgg ggctcgcacc gaacgcccac
     4321 ggcaggtccg cccagcgcca gcgagcgatg tcgttgtagc ggcgaccggg gccgtggatg
     4381 gcacgctcga ccgtgtagcc gtcgttgttg accacgagga tgatgccggg gatgcgcatc
     4441 cggatgatcg aacccagctc ggcaatgctt aactgagccg ccccgtcacc gatcacgagc
     4501 accggccgcc gctccggcgc ggcgagtgcg ccgcccagta ccgcgggcag ggtgtagccg
     4561 atggatgccc agagcggctg cccgatgaac agcacgtcgt gaggcatccg gtgcgctccc
     4621 agcccgtaga acgcggttcc ctggtcggcg aagaccagat ccccgggccg cagcgcgtcg
     4681 gtcaccacgt gccagaggtc cgcctgcgtg agcggctcgt gggtgggcac tggctcgacc
     4741 gcgggagcaa ctccgggtcc gccgtgccgc cgatgccctg ccccgaggat ccgggtgagc
     4801 acggcgagac cgtctcgcat ctcgataccc gggaaggtct gccccgcgac ggtcgccgtg
     4861 tggggaagca gatccacccg gtgcgcgtca tccagatgct gggtgaagaa gccgctcgtc
     4921 agatcggtga agtgcacgcc gacagtgatg agtcgcgccg ctgactccac cgcgcgctgc
     4981 actgcgggct cgctcgctgc gccgatgtag gtgccgagaa accgttcgtc gctctcgtcg
     5041 atcacgcgcc gaccccacag gaggcaggcg aagtccagat cggccgaacc gagaagctcg
     5101 tgcagctgcg actcggcgtg gcagcggtcc acgaggatgt ccgcgagcac gagcggcctc
     5161 ggtgcgtcgg cgagcagtgc ccgtgctgcg gcctcgaagg cgctcaggga tgcggcatcc
     5221 gagtcgggag ggtcgatcgc gagcgggctg ctgggtcgcg aggcgggcgc ctcgccgacg
     5281 tccgtgggca gcgagaggta cccaggctga gaagactgca caatcgcggt gagtacacgg
     5341 tcgatctcgg cctcggcacc gaccaggggc accatggact gcgcgacggt cacctcggcc
     5401 gtcatgcgca gcgtgtgggc gaaatccccg tcgcccaggc tgtggtgagt ggcccgcccc
     5461 gccgactgca cgagcgtcga gggagagccc acgagatgca gcagtggcac gtgctccgcg
     5521 taggcgccgg cgaccgcgtt gatggcgctc agctccccca ctccgaaggt cgtgcagacc
     5581 acaccgagtc cgcgaaggcg ggcgtagccg tcggccgcat agcccgcacc cagctcgttg
     5641 gccatggcga cccattcgac gcgggtgtcg tcatcgacgt ggtcgagcag ggcgagcgtg
     5701 aaatcgccgg ggactccgaa gatgcggtcg acgccgagct cggcgagccg gtcgagcagg
     5761 tagtcgctga ccgtgtaccg ctcggcggtc attgctcggg aacgcgctcg gatgcggtga
     5821 gctcggccgg aagcggacgc gccaccggga tcttgctgcc cagcacctgg gccacgacgt
     5881 cgcgagcgat cgcctgcggt gtgagcccga cctgctcgag caggtactcc cggctaccgt
     5941 gctcgaggaa ctggtcaggc agaccgagct cgttgacggc cgtgtccacg cccgcggccc
     6001 tcatgtcctg ccggatgcgg gtgccgatgc cgcccacgcg aacgccgtcc tcgatcgtga
     6061 tgacgagccg gtgctcggcc gcgaaggtga tgacgctcgc cggcacgggc acgacccagc
     6121 gcgggtcgat gaccgtcgcg ccgatcccct gcgcggccaa gcgctcagca acctggaggc
     6181 cgatcttcgc catcgggccg acggtcacga tgaggacatc cttgtggggc gcctcgcgga
     6241 gaacatccac accgtcgtcg gtgcgacgga tggtctcgta ctcgatgccg acactgccct
     6301 tggagaagcg cacgacggtg ggcgcgtcat ccaccgcgac cgcttcgccc agttcttcac
     6361 gaagcctggt cgcatctcgt ggagcggcaa tgcggatgtt cggtacgacc tgcaggatcg
     6421 cgagatccca catgccgtga tgactcgccc cgtcagggcc ggtgacgccg gcgcgatcga
     6481 gcacgaacgt gacgccggcc ttgtgcagcg cgacatccat cagcacctgg tcgaaggcac
     6541 gattgacgaa cgtcgcgtac aacgccacca ccgggtgcag gccgccgaag gcgagacctg
     6601 cagcggatgt cacggcgtgc tgctccgcga tgccgacgtc gagcacgcgg tccgggtact
     6661 tctccgcgaa cttgtgcagg cccgtgggac gcagcatcgc agcggtgatc cccaccagcc
     6721 gcgggttgcg gtccgcgagc tgcacgatct cgtcggcgaa cacgttggtc cacgagggct
     6781 tgctggcgac ctcgatcggc tcccccgtct cgggatcgat ctggccgacc gcgtggaact
     6841 ggtcggccgt atcgagcagg gctggctggt agccgcggcc cttctcggtg atcgcatgca
     6901 cgatcaccgg cgcgccgtag ttcttcgctt gttggagaac ttcgacgagg gcgttcaggt
     6961 cgtgaccgtg aaccggaccg aggtacttga tgtcgaggtt ggagtacagg gcctcgttgt
     7021 tgctgaagcg cgacaggaa
//
LOCUS       DAOSOU010000099         6986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10852_length_6986_cov_5.747223, whole genome shotgun sequence.
ACCESSION   DAOSOU010000099 DAOSOU010000000
VERSION     DAOSOU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10852_length_6986_cov_5.747223"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLW15_06680"
     CDS             complement(<1..324)
                     /locus_tag="PLW15_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34114.1"
                     /translation="MTVELNRVTDLDTPTAAPFGHRPANFSGFVRAKDARLVDGDGTE
                     VLLRGVGLGNWMLPEGYMWGFGPGAESPREIEALFARHAPEAAASFWEEFRDAFIDEA
                     DIALIA"
     gene            complement(321..1178)
                     /locus_tag="PLW15_06685"
     CDS             complement(321..1178)
                     /locus_tag="PLW15_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT34115.1"
                     /translation="MSTETMAGRDRSAAARARGLTLLIGVALGVGALITAFPFFWMLA
                     TSVKPQREAIDFPPTMLPQQPTLEWYGQLFTELDFGRYLVNTVIVVLIGFVGLLLMAM
                     AGYAFAKFDFRGRRWMFFLVLATMMIPVQVTMIPTYLILNGMKLTNTLVGIALPTLVS
                     GFSIFLFRQFMSTIPTEMLEAARLDGAGEFRTFWQIVLPMSGPILAVQVVLTFISGWN
                     SFLWPLIIASDQKLYTLSVGLALLNQQLAVNPSLQMAAASVMVVPILVIFIIFQRYVM
                     QGFALSGLK"
     gene            complement(1168..2046)
                     /locus_tag="PLW15_06690"
     CDS             complement(1168..2046)
                     /locus_tag="PLW15_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT34116.1"
                     /translation="MRNRLVPYFFVTPAMLLLITFGILPIAVAAVISTTNMNISAFAS
                     WNNVDFVGLDNYAKLFADSDFWQALGNTGLFAVVGVPAIVILSLGTALLLNRSQNWFF
                     RSLRAFYFLPAITAIVAISLVWSYLYNTQFGLLNYLFSLVGVPPVQWLSDPWLAKFSV
                     ALVAVWRGTGLNIIIFLAALQGVPKEYLEAASLDGAGEWRKTVSITVPLLRFAIFFVS
                     VTTTIAWLQFFDEPFVLTDGGPLGATTSVSIFLYKEGFRLNQFGYASAGSIVLFVIIA
                     AITLVQLRLRRADDEY"
     gene            complement(2126..3355)
                     /locus_tag="PLW15_06695"
     CDS             complement(2126..3355)
                     /locus_tag="PLW15_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT34117.1"
                     /translation="MKRTAKTALAIAAVAGLTLGMSACSAPAEEASGPLTVWVMGDSG
                     TNFETLVADSGIEVDVVAIPWDAIDEKLTTAVASGNGPDILQIGLSKLATFAEAGALL
                     PLDSLIADHPDLAPDNFPAGVSGTSTAVNGEIVSIPWTSDTRVLFTRTDILAENGIDA
                     PPATWDELRADAKILAARGEGQYGYYIPQWDAPLPIEMTWSMGGEVIDADGNVDFDTP
                     EFQKAVDLYTGLYADGSVPTNSDFDQTQGFISGIAPMLVSGPYLANGVSSAAPELDGK
                     WQASPLPAGDGGSISLFAGSNLGIWHNSDQVDQSLALLDYISQPEQQLAWYKLTGELP
                     TVNAALSDATLNADPNVQVYTAQLATAKVLPIVANWDGVVGTGLLNALNSIVLTGADR
                     TTALDAFFAETKGLSIK"
     gene            complement(3424..4833)
                     /locus_tag="PLW15_06700"
     CDS             complement(3424..4833)
                     /locus_tag="PLW15_06700"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOT34118.1"
                     /translation="MKTAASRDARLTSLERTTVASDFPDDFRWGLATAAYQIEGAARE
                     GGRGPSIWDTFSHTPSLSHNGDTGDIACDHYHRWNADFDLLAELGVTHYRLSVSWSRL
                     QPAGHGPLNEEAVEFYRAQLRGLADRGIRPLVTLYHWDLPQPLEDEGGWPVRSTAVAF
                     GEFATEVVRRLGDLATDWVTLNEPWCSSFLGYGFGAHAPGRTDLRAAVAAAHHLNLAH
                     GLALRGIRSVRPEARVGITNIITDIVPLTGDARDAAAVERLDATSNRVFLDPAYLGEY
                     SAAVWDALGPLGLADVVLEGDLQLISAPTDFAGVNHYQRVVAWHDDDEPFTVAQRPAE
                     PATTSFGWSVIPESLTAVLVRVSREFTALPIYVTESGASFHDYVDPNGEVIDAERVSY
                     LDGYLAAAADAIRAGVDLRGYYAWSFLDNFEWAEGYSKRFGLVYVDYRTQERIPKLSA
                     HWYAQLISDHQAVATRATA"
     gene            4998..5723
                     /locus_tag="PLW15_06705"
     CDS             4998..5723
                     /locus_tag="PLW15_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOT34119.1"
                     /translation="MLPAALIPWLDPENLIAGFGAFALLGVCLIVFAETGLLFGFLFP
                     GDTLLIITGLLTFQGVIDIWILWVCLAISLAAFLGGEVGYLIGHKAGPRIFERKESGF
                     FSIENVRRTNGFFERFGGLAVIVARFVPIVRTFAPVVAGVGHMNYRKYSLYNAAGALL
                     WGSGLTFAGYLLGYIPPLSDFVTKYIDLILLGAVALAVIPTVYHYIQSSIKARKKRNA
                     QLMDDDTVQELVLHPEDFDKKPE"
     assembly_gap    5778..5877
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5878..6137)
                     /locus_tag="PLW15_06710"
     CDS             complement(<5878..6137)
                     /locus_tag="PLW15_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT34120.1"
                     /translation="MPDVPVDLPIEQRIAQAVAELGEPTVVLRALSLIAGNNEGEEFL
                     LVVGGEHARGILDGAPVLYWPELWGTRALLYVWNDSAVIAVTA"
     gene            complement(6148..6762)
                     /locus_tag="PLW15_06715"
     CDS             complement(6148..6762)
                     /locus_tag="PLW15_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT34121.1"
                     /translation="MPGPTPTAMEDYVKVIYAHTEWQSQPITTNALAARLGLAASSVT
                     EMVKKLTAQGLAHHEPYGAIELTERGRTLALTMVRRHRLIETWLVERFGYRWDEVHDE
                     AEVLEHAMSDRLLDAIDEELGRPARDPHGDPIPAADGTLVLPDAVVLAEAASGGRVVR
                     ISDRDALLLRHLAAEGIVLDAVVSAERRAQLGEEALASIWVSAP"
     gene            complement(6762..>6986)
                     /locus_tag="PLW15_06720"
     CDS             complement(6762..>6986)
                     /locus_tag="PLW15_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOT34122.1"
                     /translation="LAIGFDPTRALVLSQVVLSFGIPFALIPLVWLASSRKEMGEFVN
                     APWLRVAGWAAAALITVLNVLLIVLVIAGG"
BASE COUNT         1175 a         2208 c         2373 g         1130 t
ORIGIN      
        1 ggcgatgagc gcgatgtcgg cctcgtcgat gaacgcgtcg cggaactcct cccagaagga
       61 tgccgctgcc tcaggcgcgt gacgcgcgaa cagtgcctcg atctcgcgcg gtgactcggc
      121 tccgggcccg aagccccaca tgtacccctc gggcagcatc cagttgccga ggcccacacc
      181 gcgcagcagc acctcggtgc cgtcgccgtc gactagccgg gcatccttgg cgcgcacgaa
      241 gccggagaag ttcgccggac ggtgaccgaa cggtgcggcg gttggggtat cgaggtcggt
      301 cacgcggttg agctccacag tcatttgagt ccggacaggg cgaatccctg catcacatag
      361 cgctggaaga tgatgaagat cacgaggatc ggaaccacca tcaccgatgc cgcagccatc
      421 tgaagcgacg ggttgacggc cagctgctgg ttgagcagcg cgagccccac cgagagcgtg
      481 tagagcttct ggtcgctggc gatgatgagc ggccaaagga agctgttcca gccggagatg
      541 aaggtgagca cgacctgcac cgcgaggatc gggcccgaca tcggcagcac gatctgccag
      601 aaggtgcgga actcgcccgc gccatcgagg cgagccgcct cgagcatctc cgtggggatc
      661 gtcgacatga actgccggaa gaggaagatg ctgaacccgc tgaccagcgt gggaagggcg
      721 atacccacga gcgtgttcgt gagcttcatg ccgttgagga tcaggtaggt ggggatcatc
      781 gtcacctgca ccgggatcat catcgtggcg agcacgagga agaacatcca ccggcggccg
      841 cggaagtcga acttggcgaa cgcgtagccc gccatggcca tgagcagcag tcccacgaag
      901 ccgatgagca cgacgatcac cgtgttgacc aggtagcgac cgaagtccag ctcggtgaac
      961 agctggccgt accactcgag cgtgggctgc tgcggcagca tcgtgggtgg gaagtcgatg
     1021 gcttcacgct gcggcttgac ggatgtggcg agcatccaga agaacgggaa cgccgtgatg
     1081 agcgcaccga cgccgagcgc cacgccgatc aggagggtca agcctctggc gcgagcggcg
     1141 gcggagcggt cccgccctgc catggtctca gtactcatcg tcggccctcc tcagccgcag
     1201 ctgcacgagc gtgatggccg cgatgatgac gaacagcacg atggaccctg cgctggcgta
     1261 gccgaactgg ttgagccgga agccctcctt gtacaggaag atcgagaccg aggtcgtggc
     1321 cccgagcggg ccgccgtcgg tgagcacgaa gggttcgtcg aagaactgca gccaggcgat
     1381 ggtcgtcgtg accgagacga agaagatggc gaacctcagc agcggcaccg tgatcgacac
     1441 ggtcttgcgc cactcgccgg caccgtcgag ggatgccgcc tccaggtact ccttgggaac
     1501 accctgaagc gccgcaagga agatgatgat gttgagtccc gtgccacgcc agacggcgac
     1561 cagcgcgacc gagaacttgg ccagccacgg gtcggacagc cactgcaccg gcggcacacc
     1621 gaccagcgag aacaggtagt taagcaggcc gaactgcgtg ttgtagaggt agctccagac
     1681 cagcgagatc gccacgatgg cggtgatcgc cggcaggaag tagaacgcgc gcaggctgcg
     1741 gaagaaccag ttctgactgc gattgagcag cagcgcggtg cccagcgaga ggatgacgat
     1801 cgcgggcacg cccacgacgg cgaagaggcc ggtgttgccc agggcctgcc agaagtcgga
     1861 gtcggcgaaa agcttcgcgt agttgtcgag cccgacgaag tcgacgttgt tccagctggc
     1921 gaacgccgag atgttcatgt tggtggtgct gatgaccgcg gcgaccgcga tgggcagaat
     1981 gccgaaggtg atcagcagca gcatcgccgg ggtgacgaag aagtagggca cgagacggtt
     2041 gcgcatcgag gtaccttcct ttcttgtcga cggcgcggat gctgcgtgaa gggcggggcc
     2101 gcacggtgtg cggccccgcc gggtactact tgatggacag acccttggtc tccgcgaaga
     2161 acgcgtcgag cgcggtggtg cggtcggcac cggtgagcac gatcgagttc agggcgttga
     2221 gcaggcccgt gccgacgacg ccgtcccagt tcgccacgat cggcagcacc ttcgcggtgg
     2281 ccagctgagc cgtgtagacc tgcacgttcg ggtcggcgtt gagcgtggca tccgacagag
     2341 cggcattgac cgtgggcagc tcacccgtga gcttgtacca agcgagctgc tgctccggct
     2401 gggagatgta gtccagcagg gccagcgact ggtcgacctg gtcggagttg tgccagatgc
     2461 ccaggttcga gcctgcgaac agcgagatgg atcctccgtc gccggcgggc agcgggctcg
     2521 cctgccactt gccgtcgagc tcgggggcgg cgctggagac accgttggcc aggtaggggc
     2581 cgctcaccag catcggggcg atgcccgaga tgaagccctg cgtctggtcg aagtcgctgt
     2641 tggtcggcac gctgccatcg gcgtagagac cggtgtacag gtcgacggcc ttctggaact
     2701 cgggggtgtc gaagtcgacg ttgccgtcgg cgtcgatgac ctcaccgccc atgctccagg
     2761 tcatctcgat cggcagcggc gcatcccact gcgggatgta gtagccgtac tgtccttcgc
     2821 cgcgagcggc caggatcttc gcgtcggcgc gcagctcatc ccaggtggcg ggcggggcgt
     2881 cgatgccgtt ctcggcgagg atgtcggtgc gcgtgaacag cacgcgggtg tcgctggtcc
     2941 acgggatgct gacgatctcg ccgttgactg cggtggacgt tcccgagact cccgcgggga
     3001 agttgtccgg cgcgaggtcg gggtggtcgg cgatgagcga gtcgagcggc agcagcgcgc
     3061 ccgcctcagc gaaggtcgcc agcttggaga gaccgatctg caggatgtcc gggccgttgc
     3121 ccgaagcgac cgccgtggtc agcttctcgt cgatcgcgtc ccacgggatc gcgaccacgt
     3181 cgacctcgat gccgctgtcg gcgacgaggg tctcgaagtt ggtgcccgag tctcccatga
     3241 cccagacggt caatggtcct gacgcctcct cggcgggcgc cgagcaggcc gacattccca
     3301 gtgtgaggcc ggccaccgcg gcgatggcca gagcggtctt cgctgttctc ttcattgagg
     3361 ttccttttct cgtgtgattt gcaagggcgg tgccggtctt ccagacttgc accttttttg
     3421 gggtcaggct gtcgcgcggg tcgcgacggc ctgatggtct gagatgagct gcgcgtacca
     3481 gtgcgcgctc agcttgggaa tccgctcctg agtgcggtag tcgacgtaga cgagaccgaa
     3541 gcgcttgctg tacccctcgg cccactcgaa gttgtcgagg aacgaccacg cgtagtagcc
     3601 gcgcaggtcg accccggccc gaatggcatc cgccgccgcc gcgaggtacc cgtcgaggta
     3661 gctgaccctc tccgcgtcga tcacctcgcc attcgggtcg acgtagtcgt ggaagctcgc
     3721 gccgctctcg gtcacgtaga tcggaagcgc cgtgaactcg cgcgacaccc gcaccagcac
     3781 cgccgtcaac gactccggga tgacggacca cccgaacgac gtcgttgcgg gctcggcggg
     3841 gcgctgggca acggtgaagg gctcgtcgtc gtcgtgccag gccaccaccc gctggtagtg
     3901 gttgacaccg gcgaagtcgg tcggagcgga gatcagctgc agatcgccct cgagcaccac
     3961 atccgcgaga ccgagtggac ccagcgcgtc ccacactgcg gccgagtact cgccgaggta
     4021 ggcggggtcg aggaagacac ggttgctggt tgcgtcgagg cgttcgacgg ccgcggcatc
     4081 ccgggcatcg ccggtgagcg gcacgatgtc ggtgatgatg ttcgtgatgc ccacgcgcgc
     4141 ctcgggtcgc accgaccgga tgccacgcag agccagtccg tgggcgaggt tgaggtggtg
     4201 cgcggccgcg actgctgccc ggaggtcggt gcgacccggc gcgtgcgctc cgaagccgta
     4261 gccgaggaag gagctgcacc acggctcgtt gagggtgacc cagtcggttg ccagatcgcc
     4321 gagtcgacgc acgacctcgg tggcgaactc gccgaaggcg acggccgtgg agcgcaccgg
     4381 ccagccgccc tcatcttcga gaggctgcgg caggtcccag tgatagagcg tgaccagcgg
     4441 gcggatgcca cggtccgcca gcccgcgcag ctgcgcccgg tagaactcga cggcctcctc
     4501 gttgagggga ccgtgacccg ccggctggag gcgcgaccac gacactgaca gccggtagtg
     4561 ggtcacgccc aactcggcca gcagatcgaa gtcggcgttc cagcggtggt agtggtcgca
     4621 ggcgatgtca ccggtgtcgc cgttgtggct gaggctgggg gtgtggctga aggtgtccca
     4681 gatgctgggt ccgcgtccgc cctcgcgggc cgctccctcg atctggtagg cggcggtggc
     4741 cagcccccag cggaagtcat cgggaaagtc gctggcgacc gtggttcgtt cgagcgacgt
     4801 cagtcgggcg tcacgggatg cggcggtctt catcgatcgt ccttggtcgg gcattggtcg
     4861 gaggggaact gacttatttc aatcattatt ataactagtg accgaagctg tcaagtcgac
     4921 ggtgccgctc acgctcaggc gtctccggca gcgacctagt accctgtcaa gaacgcccgc
     4981 gacgactgga gttcgacttg ctgcctgccg ccctcatccc ctggctcgat cccgagaacc
     5041 tcatcgcggg cttcggtgcc ttcgcgctgc tgggcgtgtg cctcatcgtc ttcgccgaga
     5101 cggggctgct gttcggattc ctcttccccg gcgacacgct tctgatcatc acgggcctgc
     5161 tcaccttcca gggtgtcatc gacatctgga tcctctgggt ttgtctggcg atcagcctcg
     5221 ccgcgttcct cggcggtgaa gtcggctacc tgatcggcca taaggcgggc ccgagaatct
     5281 tcgagcgcaa ggagtccggc ttcttcagca tcgagaacgt caggcgcacc aacggcttct
     5341 tcgagcgctt cggcggcctg gccgtcatcg ttgcccgctt cgtgcctatc gtgcgcacgt
     5401 tcgctccggt cgtggcgggc gtcggccaca tgaactaccg caagtactcg ctctacaacg
     5461 cggccggcgc cctgctgtgg ggctcgggtc tcaccttcgc cggctacctg ctgggctaca
     5521 ttccgccgct gagcgacttc gtgaccaagt acatcgacct gatcctgctg ggcgccgtcg
     5581 ctctggccgt gatccccacg gtgtaccact acatccagtc gagcatcaag gcgcgcaaga
     5641 agcgcaacgc ccagctgatg gacgacgaca ccgttcagga gctcgtgctg caccccgagg
     5701 acttcgacaa gaagcccgag tagcctcggc cgttatcagc ccgggaagcg cgacctgatc
     5761 gcgtcgattc cctgctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     5881 gtcacggcga tgacggcgct gtcgttccag acgtagagca gggcgcgcgt gccccagagc
     5941 tcgggccagt agagcaccgg agccccgtcg aggatgccgc gcgcgtgctc gccgccgacc
     6001 acgagcagga actcctcgcc ctcgttgttg cccgcgatga gcgacagggc gcgcagcacc
     6061 acggtcggct cgccgagttc ggcaaccgcc tgggcgatgc gctgttcgat gggcaggtca
     6121 acgggtacgt ctggcacggg aacaacccta cggggcgctc acccagatcg aggccagtgc
     6181 ctcctcgccg agctgcgccc gacgctcggc cgacacgaca gcatcgagca cgattccctc
     6241 cgccgcaaga tgacgcagca gtagcgcgtc acggtcgctg atgcgcacca cccgtccacc
     6301 ggatgccgcc tccgcgagca ccaccgcgtc tggcagcacg agcgtgccgt ccgccgccgg
     6361 gatcgggtca ccgtgcgggt cgcgcgcggg acgcccgagc tcctcgtcga tggcatcgag
     6421 cagccggtcg ctcatcgcgt gctcgagcac ctcggcctcg tcgtgcacct cgtcccaccg
     6481 gtagccgaag cgctcgacca gccaggtctc gatgaggcgg tggcgacgca ccatggttag
     6541 cgcgagagtg cgtccccgct cggtgagctc gatcgcgccg tagggctcgt ggtgggcgag
     6601 tccctgggcg gtgagcttct tcaccatctc agtgactgat gacgcggcca agcccagccg
     6661 cgccgcgagc gcgttcgtgg tgatcggctg actctgccac tcggtgtgcg cgtagatgac
     6721 cttgacgtag tcctccatcg cggtcggtgt cgggccgggc atcagccgcc cgcgatcacg
     6781 agcacgatga gcaacacgtt gagcacggtg atgagcgcgg ccgcagccca gcctgcgacc
     6841 cgcagccagg gagcgttgac gaactcgccc atctccttgc gcgagctcgc gagccacacc
     6901 agcggaatca gcgcgaacgg gatgccgaaa ctcagcacca cctggctgag cacgagtgcg
     6961 cgggtcgggt cgaagccgat cgcgag
//
LOCUS       DAOSOU010000100         6967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10890_length_6967_cov_5.545862, whole genome shotgun sequence.
ACCESSION   DAOSOU010000100 DAOSOU010000000
VERSION     DAOSOU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10890_length_6967_cov_5.545862"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLW15_06725"
     CDS             <1..216
                     /locus_tag="PLW15_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34123.1"
                     /translation="RPVAEAKSMRRRNAARVFTAGLVIAAFVSIPIVNLATPLFAMAF
                     MVHMHKQLSGPRPELLPPEKSAALPPR"
     gene            complement(401..1537)
                     /locus_tag="PLW15_06730"
                     /pseudo
     CDS             complement(401..1537)
                     /locus_tag="PLW15_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(1571..2047)
                     /locus_tag="PLW15_06735"
     CDS             complement(1571..2047)
                     /locus_tag="PLW15_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT34124.1"
                     /translation="MTTTRRATAADLPFLEEVFVIAADWNPEAVKGAEFWHADPTFQK
                     YLAGFPRDTDLGFIAEREGQDAGAVWSRYFTAADPGYGFVAEDIPELSIGVVAGRRGE
                     GIGRALLNAMLAASPVSLSLSVEDGNPAQELYRKSGFVPVGRVGNATTMLYTRRSD"
     gene            complement(2068..3744)
                     /locus_tag="PLW15_06740"
     CDS             complement(2068..3744)
                     /locus_tag="PLW15_06740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT34125.1"
                     /translation="MPASVFTPPQLAPGTLRVTPIGGLGEIGRNMTTFEIDGKILVVD
                     CGVLFPEENQPGVDLILPDFSPIRDRLDDVLGIVLTHGHEDHIGAVPYLLKLKGDIPL
                     IGSTLTLALIEAKLKEHRITPYTLTVSEGQHETLGPFDLEFVAVNHSIPDALAVAIKT
                     RAGVVLHTGDFKMDQLPLDDRITDLRAFARLGEAGVDLFLPDSTNADVPGFTPSERDI
                     GPVLEAVIAKAPRRVIVASFSSHVHRVQQVLDAAVANKRKVVLMGRSMVRNMGIAAEL
                     GYLKVPDGVLIDAKKAVDLPDNQLVYMSTGSQGEPMAVLARMANLEHQIEVGKGDTVI
                     LASSLIPGNENAVYRVINGLMKLGATVVHKANARVHVSGHAAAGELLYCYNILKPKNV
                     LPVHGEQRHLVANANLAIQTGVPEENTIIAEDGTVIDLRDGVAKVVGQLDLGYVYVDG
                     SSVGEITDADLKDRRILAEEGFISIFVAVDPQTGKVIVGPEIQARGFAEDENVFDAVL
                     PQIIKALTDAAENGTRDPHAFAQVIRRTVGRWVNTQHRRRPMIVPVVIEA"
     gene            complement(3767..4744)
                     /gene="dapA"
                     /locus_tag="PLW15_06745"
     CDS             complement(3767..4744)
                     /gene="dapA"
                     /locus_tag="PLW15_06745"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT34126.1"
                     /translation="MSTLPNPFGQVLVALVTPFNSDGEVNWTDVEKHIDDVITNGADG
                     IVVTGTTGETSTLTDPEKVKLVEVAKKVAGNRAKVITGGGSNETAHAIELAKKSEKAG
                     ADGNMIVTPYYNKPTQAGILTHFRMIADATDLPVILYDIPGRTGVPIKYETILRASKH
                     PNIVAVKDAKGDLAEVSRVLNQTDLYYFCGDDALVLPSLAIGATGLIGVTANIAPMPY
                     RHIVDAVNAGDLKAATAAHKSLEPLVRATMTHVPGTVATKYILHGLGRITSPRVRLPL
                     VGPEEWEAAQIEDEIDLVQDIPGVSFDNFRPDRNAAAGGALPKVAGTTR"
     gene            complement(4809..5186)
                     /locus_tag="PLW15_06750"
     CDS             complement(4809..5186)
                     /locus_tag="PLW15_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOT34127.1"
                     /translation="MSDLWQLIWNIFVIFAFVAYLMILISIFADLFRDRALNGWYKAL
                     WFIFLIVFPYLAAFIYIIARGRGMAERSAELAQARTEAANSYIRQVAGTGPAADIASA
                     KALLDEGTITEAEYEKLKKKALA"
     gene            complement(5243..5959)
                     /locus_tag="PLW15_06755"
     CDS             complement(5243..5959)
                     /locus_tag="PLW15_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOT34128.1"
                     /translation="MIMVRSALAVSAIALVATLARCSSGDAAVRSAVDPAALVGTWAI
                     DSTFDSPEQPFVAFVDDGTWTASDGCNRVQGTYELGDLGALTTTSGPSTLMACDGAQL
                     PLAVTMADYVQVSGDVLTIASSADSTITTLVRSTDPTVGPQDKPVGQWIESDAADAPF
                     LTLAADGSVHGSDGCNILNGAWTLADDGSIAFGPFASTLKFCEGVDTWLLTAATATVQ
                     GGVMTVQDAAGEVLGQLTSR"
     gene            complement(6027..>6967)
                     /locus_tag="PLW15_06760"
     CDS             complement(6027..>6967)
                     /locus_tag="PLW15_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT34129.1"
                     /translation="VIAQVKGQHPLPQSALETIATAVSVRRDLPVHFTAQRRQIGDVG
                     VALMSMSGIRVAGLAAQRSNTVHLAFLLEGRVQMTPRESAPLVLEPGACFMITNWSSF
                     DVEAREGIRALHLLIPEAVLRARGVRVKSTRFRLEGSTTLKGPLLALALSTVDPEWAP
                     STSAQRATAKALEDLAIAYLAESDDHELDRADLRAQLRKRSVEEISLRHRDAALNPAA
                     LARHLGVSLRHLQRAYEGSGTTISDQILHHRTESAVTLLSSPAGRSLTVAEQARIAGF
                     SSAYELRSAVRSKLGMLPTELRAASQDAISVPESAE"
BASE COUNT         1142 a         2204 c         2413 g         1208 t
ORIGIN      
        1 cgccccgtgg cggaagccaa gtcgatgcgg cgccggaacg cggcgcgggt cttcaccgcc
       61 ggccttgtca tcgcggcgtt cgtctcgata ccgatcgtca atctcgccac gccccttttc
      121 gccatggcct tcatggtcca catgcacaag caattgtccg gcccgcgacc cgaattgctg
      181 ccgccggaga aatcggccgc gctgccgccg cgctgacgcg aattccgcca acgcgccttc
      241 cgatcgcatg gaatcatgcg accggaaaaa atccgcgcca agtgggattg cgcgaagctc
      301 caggcttgaa gcctgtccat gcttgatgaa atggctccct ctcgtcgggc ggacggccta
      361 gacgggcttg tcgggccagc ggcagagatc ggcgatcagg cattgaccgc atttcggcgc
      421 gcgcgcgacg cagaccgagg tcgcggtcga tctcggggtc gcgcccgtct gagtccaccc
      481 accgggtgcc gtcatccccg ggcgggagcg cgagtcgact cgcgtgccgc accttgtcgt
      541 ggttggagag cacccacgtc gatgacgcac ccacgccctc cgcgagcagg acgttgtcgg
      601 tgattaccgt gcgataggtc tcgacctgcc acgggctcac gaggaggtcg aagttgaatg
      661 cctgcccgag ggagtccggc cgggcgtaga gcatgcgccg ctcggctcgc acgtaggcct
      721 cggcgacagc gacgcgcggc gggtcgtact cgttgaagac ctcgcgccac gaccggtaga
      781 tgtcgtgcac ctcattgcga tcgaagagca catcgtcacc ggtcaacggg ttgtcgaagt
      841 cgatgaaggc ggccttggaa cgcagcggct cggtcaggtc cttggtgagg gcgtgggcga
      901 catcgatcct gaagccgtcg acgccgcgat cgctccagaa tcgcagggtc gtgaggaagt
      961 cctcacgcac ctcagggtga tcccagttga agtcgggctg ctcgggcgcg aagagatgca
     1021 ggtaccagtc accatcgggc agcctgtccc acgctgtcgg tccgaagtgc gaaggccagt
     1081 cgctcggggg cagctcaccg tgctcgccga gacccttgcg gaagatgtag cgctcgcgct
     1141 ccggcgatcc cgggcccgcg gcaagcgcag cctggaacca ctcgtgctgg tcgctcgagt
     1201 ggttcggcac gatgtccacg aacattcgga tgcccactgc atggagcgca gcgatgagct
     1261 cgtcgaagtg ctccagcgtg ccgagcttcg ggtcgacctc gcggtagtgg gcgacgtcat
     1321 agccgccgtc agcgagttcg gacggataga acgggctcag ccacaccgca tcgatgccga
     1381 ggctggccaa atacggcacc cgactcgtga tgcccttgag gtctccgagg ccatcgccgt
     1441 ccgcgtcggc gaagctgcgc ggatagatct ggtagatcac ggcctggcgc caccagtcag
     1501 gatccttcga gagtacgtcg ttgtctgcag ccacaatcgt tgatgctacg gcaagcgctt
     1561 gcagtacgct ctagtcgctg cgccgggtgt acagcatcgt ggtcgcgttt ccgaccctgc
     1621 ccaccggcac gaaaccgctc ttgcggtaga gctcctgggc cgggttgccg tcttcgacgc
     1681 tcaggctgag gctgaccggg gatgccgcga gcatcgcgtt cagcagcgcc cggccaatac
     1741 cctccccgcg ccgacccgcc acaacaccga tgctgagctc cgggatgtcc tcggcgacga
     1801 acccgtagcc ggggtctgcc gccgtgaagt agcgcgacca gacggcgccg gcatcctgcc
     1861 cttcgcgctc ggcgatgaag ccgaggtcgg tgtcgcgcgg aaagccggcg aggtatttct
     1921 ggaaggtggg gtcggcgtgc cagaactcgg cgcccttgac ggcctccggg ttccagtcag
     1981 ctgcgatcac gaagacctct tcgagaaagg ggaggtcggc cgcggtggcg cggcgcgtgg
     2041 tggtcatggc gcgagtggtc ggggccctta ggcctcgatc acgaccggca cgatcatcgg
     2101 gcggcggcgg tgctgggtgt tgacccagcg gccgacggtg cggcggatga cctgcgcgaa
     2161 cgcgtgcgga tcacgggtgc cgttttctgc ggcatccgtg agggccttga tgatctgcgg
     2221 aagcaccgcg tcgaagacgt tctcgtcctc ggcgaagccg cgcgcctgaa tctcgggccc
     2281 gacgatcacc ttgccggtct gcgggtcgac ggcgacgaag atcgagatga agccctcctc
     2341 ggcgaggatg cggcggtcct tgaggtcggc atccgtgatc tcgccgacgc tggagccatc
     2401 gacgtagacg tagccgaggt cgagctgacc gacaaccttc gcgacgccgt ccctcaggtc
     2461 gatgacggtg ccgtcctcgg cgatgatcgt gttctcctcg ggcaccccgg tctggatcgc
     2521 gaggttggcg ttggcgacga ggtggcgctg ctcgccgtgc accggaagca cgttcttcgg
     2581 cttcaggatg ttgtagcagt agagcaactc ccccgcggcc gcgtggcccg acacatgcac
     2641 cctggcattg gccttgtgca cgacagtcgc gcccagcttc atgaggccgt tgatcactcg
     2701 gtagaccgcg ttctcgttgc ccgggatgag actggatgcc aggatcaccg tgtcaccctt
     2761 gcccacctcg atctggtgct cgaggttggc catgcgcgcg agcaccgcca tgggctcgcc
     2821 ctggctgccg gtcgacatgt agacgagctg gttgtcggga agatcgacgg ccttcttcgc
     2881 gtcgatcagc acgccgtcgg gaaccttgag gtagcccagc tcggcggcga tgcccatgtt
     2941 gcgcaccatc gagcgcccca tcagcaccac cttgcgcttg ttggccaccg ccgcatcgag
     3001 cacctgctgc acgcgatgca cgtggctcga gaagctggct acgatcaccc gacggggtgc
     3061 cttggcgatg acggcctcca gcacgggacc gatgtcgcgc tcgctcgggg tgaaccccgg
     3121 gacatccgca ttcgtcgagt cgggcaggaa caggtcgacg ccggcctcgc ccaagcgcgc
     3181 gaacgcccgc aggtcggtga tgcggtcgtc gaggggcagc tggtccatct tgaagtcacc
     3241 tgtgtgcagc acgacgccag ccctcgtctt gatggcgacc gcgagcgcgt cgggaatcga
     3301 gtggttgacc gcgacgaact cgaggtcgaa cgggccgagc gtctcgtgct ggccctcgct
     3361 gaccgtgagc gtgtagggcg tgatgcggtg ctccttgagc ttcgcctcga tgagcgcgag
     3421 cgtcagggtc gagccgatga gcgggatgtc gcccttcaac ttgagcaggt acggcacggc
     3481 cccgatgtgg tcctcgtgac cgtgggtgag cacgattccc agcacgtcgt cgaggcggtc
     3541 gcggatcggc gagaagtccg gaaggatcag gtcgacgccc ggctgattct cctcggggaa
     3601 gagcaccccg cagtcgacga caaggatctt gccgtcgatc tcgaaggtcg tcatgttgcg
     3661 accgatctcg ccgagaccgc cgatcggcgt gacgcggaga gttccggggg cgagctgggg
     3721 aggggtgaac actgaggctg gcatcagtgc cgagcctacc cgttggctag cgggtggtgc
     3781 ccgcgacctt cggaagcgct cccccggcgg ccgcattgcg gtcgggccgg aagttgtcga
     3841 aactcacgcc ggggatgtcc tgcacgaggt cgatctcgtc ctcgatctga gcggcctccc
     3901 actcctcggg acccacgagc ggcaggcgca cgcgagggct ggtgatgcga ccgagcccat
     3961 gcaggatgta cttcgtcgcg accgtaccgg gaacgtgcgt catggtcgcg cgcacgagcg
     4021 gctcgagcga cttgtgggcc gccgtggcgg ccttgaggtc acccgcgttg acggcatcca
     4081 cgatgtggcg gtagggcatc ggagcgatgt tcgccgtcac gccgatgagc ccggtggcgc
     4141 cgatcgcgag gctcggcagc acaagggcgt cgtcgccgca gaagtagtac aggtcggtct
     4201 ggttgagcac gcgactcacc tccgccaggt cgcccttggc gtccttgacg gcaacgatgt
     4261 tcgggtgctt cgaggcgcgc aggatcgtct cgtacttgat cggcacgccg gtgcgcccgg
     4321 ggatgtcgta gaggatcacg ggcagatccg tggcgtccgc gatcatgcgg aagtgggtga
     4381 ggatgcccgc ctgcgtgggc ttgttgtagt acggggtgac gatcatgttg ccgtcggcgc
     4441 cggccttctc actcttcttg gcgagttcga tggcatgcgc ggtctcgttg gagccaccac
     4501 ccgtgatcac cttcgcgcgg ttgcctgcca ccttcttcgc gacctcgaca agcttcacct
     4561 tctccgggtc ggtgagagtg gatgtctcgc ccgtggtccc cgtgacgacg atgccgtccg
     4621 cgccgttggt gatgacgtcg tcgatgtgct tctcaacatc ggtccagttc acttcgccgt
     4681 cggagttgaa cggcgtgacg agtgcgacga gcacctggcc gaaagggttg ggaagggtag
     4741 acacgcgtta aacgatagtg ggtggccgca gcggagaatc cgcgcggcca cccaccgaaa
     4801 gatctcgctt aggcgagggc cttcttcttg agcttctcgt actctgcctc ggtgatcgtg
     4861 ccctcgtcga gcagtgcctt ggcgctggcg atgtcggccg ccggtccggt accggcgacc
     4921 tggcggatgt acgagttcgc ggcctcggtg cgggcctggg cgagctctgc ggagcgctcg
     4981 gccatgccgc gaccgcgagc gatgatgtag atgaatgcgg cgaggtaggg gaagacgatg
     5041 aggaagatga accacagcgc cttgtaccag ccgttgagtg cacgatcacg gaacaggtcc
     5101 gcgaagatcg agatgaggat catcaggtac gccacgaagg cgaagatgac gaagatgttc
     5161 cagatgagct gccagaggtc ggacattgtt cctttttcta ttcgtgatgg tgatttgctg
     5221 atcctgccgg tgggcaggag acctaccggg acgtgagctg gccgagtacc tcgccagcgg
     5281 catcctgaac cgtcatgacg ccgccctgca cggtggcggt agcggccgtg agcagccacg
     5341 tgtcgacgcc ctcgcagaac ttgagagtcg aggcgaacgg accgaaggcg atagagccgt
     5401 catcggcgag cgtccatgcc ccgttgagga tgttgcagcc gtcgctgccg tgcacggaac
     5461 catcggcggc gagcgtcagg aacggcgcat ccgccgcgtc ggactcgatc cactgcccga
     5521 ccggtttgtc ctgcgggccg accgtcgggt cggtcgagcg caccagggtc gtgatggtcg
     5581 agtcggccga gctggcgatc gtcagcacgt cacccgagac ctgcacgtag tcggccatgg
     5641 ttacagccaa cggaagctgg gcgccgtcgc aggccatcag cgtcgagggt cccgacgtcg
     5701 tggtgagggc gccgaggtcg ccgagctcgt aggtgccctg cactcggttg cagccgtcgg
     5761 atgccgtcca cgttccatcg tcgacgaagg cgacgaaggg ctgctccggg gaatcgaagg
     5821 tggagtcgat cgcccaggtg cccaccagcg cggccgggtc gaccgccgat cgcacagcgg
     5881 catcgcccga cgagcatctg gcgagggtcg cgacgagcgc gatcgcggaa actgccagcg
     5941 cggaacggac catgatcatt gacgtctcct cagactgtga atgccagtgt ctcaataggt
     6001 gaagggtgtc gctggcgcgt ggcgcgctac tcggctgact ctggcacgga tatcgcgtcc
     6061 tgactggccg cacggagctc ggtcggcagc atcccgagct tggatcgcac ggcggagcgc
     6121 aactcgtacg ccgacgagaa ccccgcaatt cgcgcctgct cggcaaccgt gagggatcgc
     6181 ccggcgggcg acgacaggag ggtgacggcc gactcagtgc gatggtgcag gatctgatcg
     6241 gagatcgtcg tgccactgcc ctcgtaggcg cgctggaggt gtcggagtga cacgccgagg
     6301 tggcgggcca acgcggcggg gttcagcgcg gcatcccggt gacgaagaga aatctcctcg
     6361 acggagcgct tgcgcagttg ggcacgaaga tcggcgcggt ccagttcgtg atcgtcgctc
     6421 tccgcgaggt aggcgatcgc cagatcctcc agggcctttg ccgtcgcgcg ttgcgcgctg
     6481 gtcgagggcg cccactccgg atcgacagtc gacaacgcga gcgccagtag tggccccttc
     6541 agggtcgtcg aaccctcgag acggaacctg gtggacttga cgcgcacccc gcgggcgcgc
     6601 agcacggcct cgggaatcag aaggtgcagc gctcggatgc cctcgcgtgc ttcaacgtcg
     6661 aacgacgacc agttggtgat catgaagcag gcgccgggct ccagcacaag cggggcgctc
     6721 tcgcgcggcg tcatctgcac tctgccctcg aggaggaacg ccaggtgcac cgtgttcgat
     6781 cgctgcgcgg ccaagcctgc gacgcggatt cccgacatcg acatcagggc cactccgacg
     6841 tcaccgatct ggcggcgttg ggctgtgaag tgcacgggca gatcgcggcg cacgctgacc
     6901 gccgtggcga tcgtctccag cgctgactgc ggcagcggat gctgtccctt gacctgggcg
     6961 atgaccc
//
LOCUS       DAOSOU010000101         6964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_10898_length_6964_cov_5.095817, whole genome shotgun sequence.
ACCESSION   DAOSOU010000101 DAOSOU010000000
VERSION     DAOSOU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10898_length_6964_cov_5.095817"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLW15_06765"
     CDS             complement(<1..790)
                     /locus_tag="PLW15_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT34130.1"
                     /translation="MTPSSAHELATAVELAASVRAGTTTAVDATRAALARIESDDVGA
                     FQLVRAERALAEAAAVDARADRSSLPLAGVPVAIKDNIPVAGEPLRDGSAATSDAPSA
                     ADHPVVTRLRDAGAVVVGLTRVPELCVFGATDSVYGISHNPWNRSRTPGGSSGGSAAA
                     VASGFVTIAHGNDGMGSIRIPAANCGLFGLKPGAGLVPQEIGVDAWLGMSENGPLATT
                     VADAALMLSVLAARPGLATITEPATPLRIAVATNEPSGLVRTDPE"
     gene            complement(823..1602)
                     /locus_tag="PLW15_06770"
     CDS             complement(823..1602)
                     /locus_tag="PLW15_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT34131.1"
                     /translation="MTSRPVRLGIQVQPQHALYPKVRDTVRALDDMGVDILFNWDHFF
                     PLSGDKDGLHFEAWTELGSWAELTTNVEFGTLVNCNSYRNPDLQADMARTLDHISGGR
                     FIFGTGSGWFERDYDEYGYEFGTAGSRLDALAEALPRIESRWQKLNPAPLRDIPILIG
                     GSGEQKTLKLVAKHADIWHSFVPPAELPRKIGILQEHCDTVGRDISEIEFSSEQRVKD
                     LATADELRELGVTLFTIGISGPDYPLDIVREWLVWRDQQNA"
     gene            complement(1710..3263)
                     /gene="ffh"
                     /locus_tag="PLW15_06775"
     CDS             complement(1710..3263)
                     /gene="ffh"
                     /locus_tag="PLW15_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT34132.1"
                     /translation="MAIFGNLTDRLADTFKNLRGKGKLSPADVDGTVREIRRALLDAD
                     VALEVVKDFTGRVRERALGDEVSKALNPAQQVVQIVNDELVAILGGEARRIQFAKQPP
                     TVIMLAGLQGAGKTTLAGKLAKWLKSEGHTPVLVAADLQRPNAVTQLQVVGEQAGVPV
                     FAPEPGNGKGDPVKVAKEALKHAKSKQYDVVIVDTAGRLGVDAELMKQASNIRKAIDP
                     DEVLFVIDAMIGQDAVTTARAFQEGVDFTGVVLSKLDGDARGGAALSVASVTGRPILF
                     ASTGEGLDDFEPFYPDRMASRILDLGDILTLIEQAQKTFDEEESRKVAEKFATDTFTL
                     EDFLGQMQQLKNMGSIKNMLGMLPGAKGMREQLDNFDEREITRTEAIIQSMTLQERRQ
                     PKVLNGSRRVRIARGSGTTVTEVNALVNRFEQAAKMMKTVAKGGTPQIPGMGPIPGQK
                     FGGSKQQPKKKGSRSGNPAKRADENAALAAGASAKPAAAAGSGFGLGSKPGGPSEEEL
                     ASLQKFLGR"
     gene            3309..3827
                     /locus_tag="PLW15_06780"
     CDS             3309..3827
                     /locus_tag="PLW15_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOT34133.1"
                     /translation="MPLIVPFGGSTPQVADTAWVAPNATLIGDVTLHERSSVFYGTVL
                     RADVASITLGERSNLQDNVTVHCDSGVPTTIGSGVSVGHAATLHGCTIEDDCLIGMGA
                     TVLNHAVVGTGSLVAAGTVVLEGTIVPPGSLVAGVPGKVRRELTDEERAGIRANGERY
                     VQYAVAHREATG"
     gene            3827..4471
                     /locus_tag="PLW15_06785"
     CDS             3827..4471
                     /locus_tag="PLW15_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34134.1"
                     /translation="MRGASLVGVLALTAVLAGCSAPAPVPGGSDSAPTAAAGAPSSSP
                     TATQSPTAADFAVPSTCDGLLPPELAASFASQGLTGSEVAVFGDVAVGDPIPVLDYDT
                     NVDSGIVMTDYLFCEWTGSDQTIDIFLGRVDATSRPLVVSEFWSFDRRIDRTAATVVM
                     TFDGMIPGVGQGPANYWALRTNSELKVDVSPGDEQARQLAQSIADQVAAHVMAD"
     gene            complement(4492..5193)
                     /locus_tag="PLW15_06790"
     CDS             complement(4492..5193)
                     /locus_tag="PLW15_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT34135.1"
                     /translation="MFSSAVTTYWKPESAPEISDDFTVVIDPTLSDSRSVSVLSVADG
                     RRLLTVTPARAAELALSPAATVSDEELARRLAAASLELNGPDHLFYLPVAQREVLRLE
                     PVGAQTRPLTDEDAALFERFTQEAPEDDLDDAFVELDHWLVFGTILDGRLVSAASMYP
                     WDGSRLADLGVITLPEFRGRGLGRTTVRAMSALAIDRGYEPQYRCQFDNVSSVALAAS
                     AGFALFGDWDVVATE"
     gene            complement(5203..5502)
                     /locus_tag="PLW15_06795"
     CDS             complement(5203..5502)
                     /locus_tag="PLW15_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34136.1"
                     /translation="MKTTRGVIPLIAAIVGVAAMSWAFVTGISAALSGGGSGALPYEI
                     VFIVAALVVLAALVLAIVNLVKGNSRALAVVTIIVALVPLVAVILIRIAADPGFQ"
     gene            complement(5508..6374)
                     /gene="ftsY"
                     /locus_tag="PLW15_06800"
     CDS             complement(5508..6374)
                     /gene="ftsY"
                     /locus_tag="PLW15_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT34137.1"
                     /translation="MASWSLSGALKGMFSRATIDEETWEHLEDALISADFGPDITDSI
                     VEELRASVAKFRTTDPADLKRMLRETLEERLARLDPTLHLSARPAVVLVVGVNGVGKT
                     TTIGKFAKFLTGYGRTVVVGAADTFRAAAVEQLATWAERGGAAIVKPQQQGQDPASVA
                     FQTVERAQREGIEIVLIDTAGRLQTKSGLMDELGKIRRVLEKQTEIAEVLLVLDATTG
                     QNGLAQAEAFIEHAGVTGLVLTKLDGSAKGGFVLAVQEKTGIPIKLVGQGEGIGDLTG
                     FTPHVFVQGLVG"
     gene            complement(6415..>6964)
                     /locus_tag="PLW15_06805"
     CDS             complement(6415..>6964)
                     /locus_tag="PLW15_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34138.1"
                     /translation="PGLLLVAVLTLSGCGTEPAPTAPLTSSTPTVVPTRTASPTPTPT
                     PTVKSGWSECPKIVQRLDATAPEGTRYYEVEPKKFAVQLVGTAVLAKACVVTADVGGE
                     LSTWAILPGDSELADSVARDLLGGGFTAKGGGAYTNTDTGYGVLVRFAGSGKSLDTTL
                     DLVDVFAPYNQPLVSLSTFGLS"
BASE COUNT         1076 a         2342 c         2425 g         1121 t
ORIGIN      
        1 actcggggtc cgtacgcacg aggcccgacg gctcgttggt cgcgaccgcg atccgcagcg
       61 gggttgcggg ctcggtgatg gtggcgaggc cggggcgcgc ggccagcacc gagagcatga
      121 gcgccgcatc cgccacggtc gtcgcgagcg ggccgttctc gctcatgccg agccaggcat
      181 caaccccgat ctcctgcggc acgaggccag cgccgggctt gagcccgaag aggccgcagt
      241 tggcggccgg aatacggatc gaacccatgc cgtcgttgcc gtgtgcgatc gtgacgaatc
      301 cggatgccac ggctgcggca cttccccccg acgagccgcc cggggtgcgg ctgcggttcc
      361 acgggttgtg gctgattccg tacaccgaat cggtggcacc gaacacgcag agctcgggca
      421 cccgggtgag tccgacgacc acggctcccg cgtcgcggag acgggtgacg acggggtggt
      481 ccgccgccga tggcgcgtca gaggtcgcgg ctgagccgtc gcgcagtggc tcccccgcga
      541 ccgggatgtt gtccttgatc gccaccggca cgccggccag cggcaggctg gagcgatcgg
      601 cacgggcatc caccgcggcg gcctcggcga gtgcccgctc agcgcgcacc agctggaacg
      661 ccccgacgtc gtcggactcg atgcgagcca gggcagcacg ggtcgcgtcg acagcggtgg
      721 tggtgccggc gcgcacggaa gcggcgagct cgacggcggt ggcgagttca tgggcagacg
      781 acggtgtcat ggcctcatcg taggccgcgg ccgagccccg cgtcaggcgt tctgctggtc
      841 gcgccagacg agccactcgc gcacgatatc gagcgggtag tcgggcccgg agataccgat
      901 cgtgaacagg gtgacgccca gctcgcgcag ctcgtctgcc gtggccagat ccttcacccg
      961 ctgctcgctc gagaactcga tctcgctgat gtcgcgaccg accgtgtcgc agtgctcctg
     1021 caggatgccg atcttgcgcg gcagctcggc cggcggcacg aagctgtgcc agatgtcggc
     1081 gtgcttggcg acgagcttga gcgtcttctg ctcgccgctg ccgccgatga ggatcgggat
     1141 gtcccgcagg ggcgcggggt tcagcttctg ccagcgggac tcgatgcggg gcagtgcttc
     1201 ggcgagcgca tccagtcggc ttccggcggt gccgaactcg tagccgtact cgtcgtagtc
     1261 acgctcgaac caccccgagc cggtgccgaa gatgaaccgg ccgcccgaga tgtggtcgag
     1321 ggtgcgggcc atgtcggcct ggaggtcggg gttgcggtag ctgttgcagt tgacgagggt
     1381 gccgaactcg acgttggtcg tgagttcggc ccacgagccg agctcggtcc acgcctcgaa
     1441 gtgcagcccg tctttgtctc cggagagcgg gaagaagtgg tcccagttga agaggatgtc
     1501 gacgcccatg tcatcgagcg cgcgaacggt gtcgcgaacc ttggggtaga gggcgtgctg
     1561 aggctgcacc tggataccca ggcggacggg acgtgaggtc atgcttccac gctaccccgc
     1621 gcgcatggtt cggacatttc ggggcgccgt ttcatccgcg cgggtccaca gtgtgcgcgc
     1681 gaggccgcga gatcgcgccg cgcgggcggc tagcgcccga ggaacttctg caggctcgcc
     1741 agttcttctt cgctcggacc gcccggcttc gaccccagac cgaagcccga gccggccgcg
     1801 gcagcgggct tggcggatgc cccggccgcg agcgccgcgt tctcgtccgc gcgcttggcg
     1861 gggttgcccg agcgggaacc cttcttcttc ggctgctgct tggagccacc gaacttctgg
     1921 ccggggatcg gacccatgcc ggggatctgc ggcgtgccgc ccttggccac ggtcttcatc
     1981 atcttcgccg cctgctcgaa gcggttcacg agcgcgttga cctcggtgac cgtggtgccc
     2041 gaaccacgcg cgatgcgcac ccggcgggac ccgttgagca ccttgggctg acgccgctcc
     2101 tgcaacgtca tcgactggat gatcgcctcg gtgcgggtga tctcgcgctc gtcgaagttg
     2161 tcgagctgct cgcgcatgcc cttcgcaccg ggcagcatgc cgagcatgtt cttgatcgag
     2221 cccatgttct tgagctgctg catctggccc aggaagtcct cgagcgtgaa ggtatcggtc
     2281 gcgaacttct cggcgacctt gcgcgactcc tcctcgtcga acgtcttctg cgcctgctcg
     2341 atgagggtga ggatgtcacc gagatcgagg atgcgactgg ccatgcggtc gggatagaac
     2401 ggctcgaagt cgtccagacc ctcgccggta ctcgcgaaca ggatcgggcg gcccgtgaca
     2461 ctcgccaccg acagcgcggc tccaccgcgg gcatccccat cgagcttgga cagaacgaca
     2521 cccgtgaagt cgacgccctc ctggaaggcg cgtgccgtgg tgaccgcgtc ttgaccgatc
     2581 atcgcgtcga tgacgaagag cacctcgtcg gggtcgatgg ccttgcggat gttgctggcc
     2641 tgcttcatga gctcggcatc gacgccgaga cggccggccg tgtcgacgat gaccacgtcg
     2701 tactgcttgg acttggcgtg cttcagcgcc tccttggcga ccttcaccgg gtcacccttg
     2761 ccgttgccgg gctcgggagc gaacacggga acgcccgcct gctcccccac cacctgcaac
     2821 tgcgtgacgg cgttgggacg ctggaggtcg gccgcgacca gcaccggcgt gtggccctcg
     2881 gacttcagcc acttcgcgag cttgcccgcg agcgtcgtct taccggcgcc ctggagaccc
     2941 gcgagcatga tcaccgtggg cggctgcttc gcgaactgga tgcgcctggc ctcgccaccg
     3001 agaatcgcca ccagctcgtc gttgacgatc tgcacgacct gctgcgccgg gttcagtgcc
     3061 ttggacacct cgtcgccgag cgcgcgctcc cgcacccgcc ccgtgaagtc cttgacgacc
     3121 tcgagggcga cgtcggcatc gagcagggcg cgacggatct cgcggacggt accgtcgaca
     3181 tccgcggggc tgagcttgcc cttgccgcgg aggttcttga acgtgtcggc gagacggtcg
     3241 gtgaggtttc cgaagatggc catggtgcag ccagtttatc cgggcgggag ccgagtaccc
     3301 tgattcgcat gcccctcatc gtgccgttcg gcggctccac gccccaggtt gccgacaccg
     3361 cctgggttgc acccaacgcg acgctgatcg gtgacgtgac gttgcacgag cgctcgagcg
     3421 tcttctacgg aaccgtgctg cgggccgacg tcgcatccat cactctcggc gagcgcagca
     3481 acctgcagga caacgtgacg gtgcactgcg acagcggcgt gcccacgact atcggctcgg
     3541 gcgtgagcgt cggccacgcg gccaccctgc acggctgcac catcgaggac gactgtctca
     3601 tcggcatggg tgcgactgtg ctcaaccacg cggtggtcgg caccggctcg ctggtggctg
     3661 ccggcaccgt cgtgctcgag ggcacgatcg tgccgcccgg gtcgctcgtc gcgggggtac
     3721 ccggcaaggt gcggcgcgag ctgacggacg aggagcgggc gggcatccgc gccaacggcg
     3781 aacggtacgt gcagtacgcg gtcgcgcacc gggaggcgac cggctgatgc gaggggcgtc
     3841 gctggtcggc gtactggcac tgacagcggt gctggcggga tgctcggcgc ccgcccccgt
     3901 gcccggaggc tccgactccg cccccaccgc ggctgccgga gcgccctcct cgtcacccac
     3961 ggccacgcag tcccccaccg cggctgactt cgcggtgccg tcgacctgcg atggcctgct
     4021 gccgccggag ctcgctgcat cgttcgcctc acagggcctc acggggagcg aggtcgcggt
     4081 gttcggcgac gtcgccgtcg gcgatccgat ccccgtgctc gactacgaca ccaacgtgga
     4141 cagcggcatc gtcatgacgg actacctgtt ctgcgagtgg accggttccg accagaccat
     4201 cgacatcttc ctgggccgcg tcgacgccac cagccgcccg ctcgtcgtca gcgagttctg
     4261 gtcgttcgac cgacgcatcg accgcaccgc tgccacggtg gtgatgacct tcgacgggat
     4321 gatccccggc gtcgggcagg gaccggcgaa ctactgggca ctgcgcacca actcggaact
     4381 caaggtcgac gtctcgccgg gagacgagca ggcacgccag ctggcgcagt cgatcgccga
     4441 tcaggtcgcc gcccacgtga tggccgactg aacctgcccg ggcgactgca gctactcggt
     4501 agcgacgacg tcccagtcac cgaagagcgc gaatcccgcg gatgccgcga gcgcaacgga
     4561 tgagacgttg tcgaactggc accggtactg cggctcgtag ccccggtcga tcgccagggc
     4621 gctcatcgcc cgcacggtcg tacgccccag accacgacct cggaactcgg ggagggtgat
     4681 gacaccgagg tcggccagtc gggagccgtc ccacgggtac atgctggccg ccgaaaccag
     4741 acggccgtcg aggatcgttc cgaacacgag ccagtggtcg agctcgacga aggcgtcatc
     4801 gaggtcgtct tcgggcgcct cctgagtgaa tcgctcgaac agcgcggcgt cttcatcggt
     4861 caggggccgg gtctgcgcac cgaccggctc gagtctgagc acctcccgct gggcgaccgg
     4921 gaggtagaag aggtgatccg gcccgttgag ttcgagactg gcggcggcca ggcgtcgggc
     4981 caactcctcg tcgctgacgg tcgctgccgg ggagagcgcg agctcggcgg cacgcgcggg
     5041 tgtgactgtc agcagcctcc tgccgtcggc gaccgacagc accgagaccg aacgggagtc
     5101 gctgagggtc ggatcgatga cgaccgtgaa gtcatcggag atctcgggtg ccgactccgg
     5161 cttccaatag gtcgtgacgg cggaggagaa cacgccgaaa ggttactgga agcccgggtc
     5221 ggctgcgatg cggatcagga tcacggccac caagggcacc agcgccacga tgatcgtcac
     5281 gacggcgagg gcgcgggaat tgcccttcac caggttgacg atcgcaagca ctaaggccgc
     5341 cagcaccacg agggccgcga cgatgaacac gatctcgtag ggcagcgcgc cgctgccccc
     5401 accggagagc gccgccgaaa tgccggtgac gaacgcccag gacatcgctg cgacaccgac
     5461 gatggccgcg atcagcggga tgactccgcg agtagttttc acgtcgctca gcctacgaga
     5521 ccctgaacga acacgtgagg ggtgaagccg gtgagatctc cgatgccctc gccctgaccg
     5581 acgagcttga tcgggatgcc cgtcttctcc tgcacggcga gcacgaaccc gcccttcgcc
     5641 gagccgtcga gcttggtgag cacgaggccc gtcacgcccg cgtgctcgat gaacgcctcc
     5701 gcctgcgcta ggccgttctg cccggtcgtc gcgtcgagca cgagcagcac ctcggcgatc
     5761 tctgtctgct tctccagcac ccggcggatc ttgccgagct cgtccatcag gccactcttc
     5821 gtctgcagcc gccccgcggt gtcgatgaga acgatttcga ttccctcgcg ctgcgcacgc
     5881 tcgacagtct ggaacgcgac ggaggccgga tcctgaccct gctgctgcgg tttgacgatc
     5941 gccgctcccc cgcgctcggc ccaggtggcg agttgctcga ccgcggcagc gcggaacgtg
     6001 tccgcggcgc cgacgacgac ggtgcggccg tagccggtga ggaacttcgc gaacttgccg
     6061 atcgtggtgg tcttgccgac accgttgacg ccgacgacga gcacgacggc gggtcgcgcc
     6121 gagagatgga gggtcgggtc gaggcgggct aggcgttcct cgagggtctc gcggagcatc
     6181 cgcttcagat cggcgggatc tgtcgtgcgg aacttggcga cgctcgcgcg cagctcctcg
     6241 acgatcgagt cggtgatgtc ggggccgaag tccgccgaga tcagcgcgtc ttcgaggtgc
     6301 tcccaggtct cctcatcgat cgtcgcgcgg gagaacatgc ccttgagcgc gccggaaagc
     6361 gaccacgatg ccatggctca agggtagggc accctcggcg ggttgcccgg gaggtcagga
     6421 caggccgaat gtggacagcg agaccagcgg ctggttgtac ggggcgaaga cgtcgacgag
     6481 gtcgagggtc gtgtccagac tcttgccgga gccggcgaac cgcaccagga cgccgtaacc
     6541 cgtgtcggtg ttcgtgtagg caccaccacc cttggcggtg aacccgccgc ccagaagatc
     6601 gcgggcgacg gaatcagcga gttcgctgtc gcccgggaga atcgcccacg tcgacagctc
     6661 gccgcccacg tcagcggtca ccacgcaggc cttggcaagc accgcggtgc ccaccagctg
     6721 cacagcgaac ttcttcggtt cgacctcgta gtaccgagtg ccttcgggcg cggtggcgtc
     6781 gaggcgttgc acgatcttcg ggcactcgct ccatccgctc ttcaccgtcg gagttggtgt
     6841 tggagtcggt gacgcggtcc gcgtgggcac gacggtgggt gtcgacgacg tgaggggcgc
     6901 ggtcggtgct ggctcggtgc cgcagcccga cagggtgaga acagcaacaa gtagtagtcc
     6961 cgga
//
LOCUS       DAOSOU010000102         6796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_11258_length_6796_cov_4.949859, whole genome shotgun sequence.
ACCESSION   DAOSOU010000102 DAOSOU010000000
VERSION     DAOSOU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11258_length_6796_cov_4.949859"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PLW15_06810"
     CDS             complement(<1..1418)
                     /locus_tag="PLW15_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOT34139.1"
                     /translation="MASEPLLERAPRPWNADAAYDAFEGWASGRGLPLYPAQDEALIE
                     LVSGANVILSTPTGTGKSLVAIAAHFVALAQGKRTFYTAPIKALVSEKFFALVDIFGA
                     ENVGMVTGDSSVNPDAPIICCTAEILANLALRHGAHAPVDQVVMDEFHFYADPDRGWA
                     WQVPLLTLPRVQFLLMSATLGPMKELADDLTRRTGRETSLITGVDRPVPLHYSYALTP
                     VHETVEDLLHTGQSPIYIVHFSQAAALERAQAMTSIKIVSREHRDEIAEAIGSFRFTT
                     VFGQTLSRLIRAGIGVHHAGMLPKYRRLVEQLAQRGLLRVICGTDTLGVGINVPIRTV
                     LLTALTKFDGTRMRQLSAREFHQIAGRAGRAGYDTAGTVVAQAPEHEAENARLVAKAG
                     DDIKKVRKIVRKKAPEGFVSWSQTSFERMIAAEPETLTSHMQVSHSMILNVIARGGDA
                     FQAMRDLIFDSHETWNNKLALAR"
     gene            1472..2296
                     /locus_tag="PLW15_06815"
     CDS             1472..2296
                     /locus_tag="PLW15_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34140.1"
                     /translation="MAMNISSLDVIPVDGGTVAYEIAGAGPLVVCVPGMGDLRSTWRH
                     LAPSLVAAGYRVALTDLRGHGDSSTDFAEYDDEATARDIIALVEHLGEPAVIIGNSMG
                     AGSAVIAAAARPELVRGLVLVGPFVRNPPSVGVAMRLAFRVLMARPWAAAVWKGYLPS
                     LYKGRAPEDQAEYLATVAAALKRPGYGAAFSRTTRTTHAPAEAALPAVSAPALVIMGE
                     LDPDFPDPAAEAAWIGEQLSATVVMVAEAGHYPQSQRPDVVVPAVESFLAGLGTNA"
     gene            2319..2852
                     /locus_tag="PLW15_06820"
     CDS             2319..2852
                     /locus_tag="PLW15_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HOT34141.1"
                     /translation="MVNEAERLADEVGLAQLSLAALAARLGVQPPSLYKHIAGQDALQ
                     RTIEARAKAELAEVMRDAAVGRSGADAVRSIAAEYRRWAKAHPGRYAATLAAPDPADA
                     DALAASVRAVDVLRGALASYRLVGDDEIDAIRTLRSGLHGFVALEAAGGFGLPLDVDR
                     SFERLVDALVATLPSGR"
     gene            2886..3254
                     /locus_tag="PLW15_06825"
     CDS             2886..3254
                     /locus_tag="PLW15_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34142.1"
                     /translation="MPHVTDLRPFIPALDFAASIDFYRGLGWSVDEVGDGLALVEASE
                     QHFYVQDFADRRIAENTMLHFTVEDAAAWFDLVSAQIAANPASGARVQAPKRQDYGAL
                     VTFVHDPAGVLLHFCQWDSA"
     gene            complement(3256..4185)
                     /locus_tag="PLW15_06830"
     CDS             complement(3256..4185)
                     /locus_tag="PLW15_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOT34143.1"
                     /translation="MARSILIDCDPGLDDALALLLAHGDPNLNLVGVTTVGGNVGLET
                     TTRNALQLREYLGFDKVPVAAGAAEPLTRQVRDAAHVHGAGGLGDVVLPEAVLEAHDD
                     HAVDFIIDTLRAAPGSIHLVATGPLTNIALALEREPAIARWVASFTIMGGSYTRGNAT
                     VAAEFNIWADPEAARIVFDADWRVTMIGLDLTLQAQANAAVISRLKELGKLADHLIVP
                     LATFYFNPNDEDWDGQAVHDVCAVAYVARPDLFTVRPARVDIETTGEFTTGMTVVDFD
                     PVVPNATVATHLDVPGFWAYVENVYARVAREHS"
     gene            4282..5682
                     /locus_tag="PLW15_06835"
     CDS             4282..5682
                     /locus_tag="PLW15_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT34144.1"
                     /translation="MAELDEEEPDFVTVRRATARMFKAVKKTRRLEKRAEIAESDRSV
                     IAATATGAPDRIDDETRGIPISTSTTAPTAGTLIKARACYICKQPYTLVDAFYHQLCP
                     DCAALSHAKRDARTDLTGRRALLTGGRAKIGMYIALRLLRDGAHTTITTRFPRDAVRR
                     FTSLPDSPEWIDRLRIVGIDLRDPAQVIGLADSVAAAGPLDILINNATQTVRRSPGAY
                     QPLVDAELAPLPDGPLPELVTFGHTNDQHPLALERSLAAHPILAAAADVSAAAQELAD
                     QAMGAGNSSLERLAAGTAIDAGGLLPDLDDVNSWTQAVHEVDPLEMLEVQLANTTAPF
                     ILISRLRPSMAAAAARRKYVVNVSAMEGVFGRGYKGPGHPHTNMAKAAVNMLTRTSAR
                     EMFETDGILMTSVDTGWITDERPHPTKVRLAEEGFHAPLDLVDGAARVYDPIVRGEAG
                     EDVFGVFLKDYAPSSW"
     gene            5679..5891
                     /locus_tag="PLW15_06840"
     CDS             5679..5891
                     /locus_tag="PLW15_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34145.1"
                     /translation="MSAALVNLEPGMRVVIRYRLDDGRATDALGILLSLTSSHAVVDT
                     KRGAESIELVRVIASKEVPPPPRPRP"
     gene            5925..>6796
                     /locus_tag="PLW15_06845"
     CDS             5925..>6796
                     /locus_tag="PLW15_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT34146.1"
                     /translation="MATLPESSRWKRKPVDHCDVVMKGGITSGVIYPRALVELARHYR
                     FRGMGGSSAGAIGAALGAAAEFGRSRGGFARLYELPDQLDLGSLFRPERSTRALLPIL
                     MAFTGFRPDGGQRTGFARALAIVGAIASGFPLAGILGLLPGVGLIIAGALADSWPGYL
                     LIPSGAVLAIIGWIVAVGIRLGWKLTRSVPANLFGICRGLDPTGGDDGLTNWLSTKLD
                     LIAGLTAEEGPLRFGHLWTGRRNFRAGSIGDIPAFERSIDLRMISTCLSRSRPYEMPL
                     KARTFLYDPAVWATL"
BASE COUNT         1056 a         2370 c         2293 g         1077 t
ORIGIN      
        1 cgcgcgaggg cgagcttgtt gttccacgtc tcgtggctgt cgaagatgag gtcgcgcatc
       61 gcctggaatg catcgccgcc gcgggcgatc acgttgagga tcatcgaatg gctgacctgc
      121 atgtgactgg tcagagtctc gggttcggcg gcgatcatcc gctcgaagct cgtctggctc
      181 caggagacga aaccctccgg agccttcttg cgcacgatct tgcgcacctt cttgatgtcg
      241 tcgccggcct tggcgaccag gcgcgcgttc tcggcttcgt gctcgggagc ctgggcgacg
      301 acagttcccg cggtgtcgta gcccgcacga ccggcacggc ccgcgatctg gtggaactcg
      361 cgggcgctga gctggcgcat ccgggtgccg tcgaacttgg tgagcgccgt gagcagcacc
      421 gtgcggatcg gcacgttgat gcccacgcca agggtatcgg ttccgcagat gacgcgcagc
      481 agaccgcgct gagcgagctg ctcgacgagc cggcggtact tgggcagcat gcccgcatgg
      541 tgcacaccga tgccggcgcg gatgaggcgg gacagggtct gaccgaacac cgtcgtgaag
      601 cggaacgagc cgatcgcctc agcgatctcg tcgcgatgct cccggctgac gatcttgatg
      661 ctcgtcatcg cctgcgcccg ctccagagcc gcggcctgcg agaagtgcac gatgtagatc
      721 ggcgactggc cggtgtgcag cagatcctcg acagtctcgt gcaccggcgt gagcgcgtag
      781 gagtagtgca gtggaacggg tcggtcgaca ccggtgatca ggctcgtctc acgcccggtg
      841 cggcgagtga ggtcgtcggc gagctccttc atcgggccga gcgtcgccga catgagaagg
      901 aactgcacac gcggcagcgt cagcaggggc acctgccacg cccagccgcg gtccgggtcg
      961 gcatagaagt ggaactcgtc catcacgacc tgatcgaccg gtgcgtgggc tccgtggcga
     1021 agggcgaggt tggcgaggat ctccgccgtg cagcagatga tcggggcatc cgggttgacg
     1081 ctcgaatctc ccgtgaccat tccgacgttc tcggcgccga agatgtcgac gagcgcgaag
     1141 aacttctcgc tcacgagggc cttgatcgga gcggtgtaga acgtgcgctt gccttgagcg
     1201 agggcgacga agtgcgccgc gatcgccacc agtgacttgc ccgtgccggt cggagtgctg
     1261 aggatcacat tcgcgcccga caccagctcg atcagcgcct cgtcctgcgc ggggtagagc
     1321 gggagcccgc gcccggatgc ccagccctcg aacgcgtcgt acgcggcatc cgcgttccag
     1381 gggcgcggag cgcgctcgag aagcggttcg gatgccatgg ctcaaccgtg ccacatcccc
     1441 cttgacaccg ttggctattc tcattagctt tatggctatg aacattagct cactggatgt
     1501 gatccccgtc gacggcggta ccgtcgccta cgagatagcg ggcgccggcc ctctcgtcgt
     1561 gtgcgtcccc ggcatgggcg acctgcgctc cacctggcgc cacctcgccc cctcactcgt
     1621 cgccgcgggc taccgcgtcg cgctcaccga cctcagggga cacggcgaca gcagcaccga
     1681 cttcgccgag tacgacgacg aagccaccgc ccgggacatc atcgccctcg tcgagcacct
     1741 cggcgagccc gccgtcatca tcggcaactc gatgggcgca ggctctgcgg tcatcgccgc
     1801 agcggcacga ccggagctgg tgcgcggact cgtgctcgtg gggccgttcg tgcgcaaccc
     1861 accgagcgtc ggcgtcgcca tgcgtctggc gttccgcgtg ctgatggcgc gtccgtgggc
     1921 cgccgcggtc tggaagggct acctgccgtc gctgtacaag ggccgcgcgc ccgaagacca
     1981 ggctgagtac ctggccacgg ttgccgccgc cctgaagcgc ccggggtacg gcgcggcctt
     2041 ctcgcgcacc acccgcacga ctcacgcccc agccgaggcc gcgctaccgg cagtctcggc
     2101 accggctctg gtcatcatgg gcgaactcga ccccgacttc cccgatcccg cggctgaggc
     2161 cgcgtggatc ggcgagcagc tgtccgccac cgtcgtgatg gttgcggagg ccggccacta
     2221 cccgcagtcg cagcggcccg atgttgtggt gccggcagtg gaatccttcc tggccggttt
     2281 gggcacgaat gcctagggcc ggcttgaccg cagagcgggt ggtgaacgag gcagagcgcc
     2341 tcgccgatga ggtggggctt gcgcagctca gcctggccgc cctcgccgct cgcctcggag
     2401 tgcagccccc cagcctgtac aagcacatcg cgggtcagga tgccctgcag cgcaccatcg
     2461 aggcgcgggc gaaggccgaa ctggccgagg tgatgcggga tgccgcagtc gggcgatccg
     2521 gggcagacgc ggtgcgatcc atcgccgccg agtaccgccg ctgggcgaag gcccacccgg
     2581 gacggtacgc ggcaacactt gctgccccgg accccgctga cgccgacgcg ctcgcggcga
     2641 gcgtcagggc tgtcgacgtg ctgaggggcg cactggcctc ttaccggctc gtcggtgacg
     2701 atgagatcga cgcgatccgc acgcttcgat ctggcctgca cggcttcgtc gccctcgagg
     2761 cggccggtgg attcggtctc cccctcgacg tcgaccgcag cttcgaacga ctcgtcgatg
     2821 ccctggtcgc gaccctcccg tcgggccgct agcaggtgtg tggcgtgcgg gaatagcctg
     2881 atgtgatgcc ccacgtgacc gaccttcgcc cgttcattcc cgcgctcgac ttcgccgcgt
     2941 cgatcgactt ctatcgtggg ctcgggtggt cggtcgacga agtgggcgac ggtcttgccc
     3001 tggtggaggc atccgagcag cacttctacg tgcaggactt cgctgaccgg cggatcgccg
     3061 agaacacgat gctccacttc accgtcgagg atgccgcggc ctggttcgac ctcgtcagcg
     3121 cgcagatcgc cgccaacccc gcctccggcg cgcgagtgca ggctccgaag cgccaggact
     3181 acggcgcact cgtcacgttc gtgcacgacc ccgccggcgt gctgctgcac ttctgccagt
     3241 gggacagcgc ctgacctaac tgtgctcgcg cgccacccgc gcgtacacgt tctcgacgta
     3301 cgcccagaac ccgggcacgt cgagatgagt ggcgacggtc gcgttcggca ccacggggtc
     3361 gaagtccacc acggtcatcc ccgtggtgaa ttcacccgtg gtctcgatgt cgacgcgcgc
     3421 gggtcgcacc gtgaacaggt cggggcgcgc cacgtacgcc acggcacaga cgtcgtgcac
     3481 cgcctgaccg tcccaatcct cgtcgttcgg gttgaagtag aaggtcgcaa ggggaacgat
     3541 gagatggtcg gcgagcttgc cgagctcctt gaggcgcgag atcacggcgg cgttggcctg
     3601 agcctgaagg gtgagatcga gaccgatcat cgtgacccgc cagtcggcgt cgaacacgat
     3661 gcgggcggcc tcagggtcgg cccagatgtt gaattccgcc gcgaccgtcg cattgccgcg
     3721 ggtgtacgaa ccgcccatga tcgtgaacga cgcgacccac cgcgcgatcg cgggctcgcg
     3781 ctcgagtgcg agagcgatat tggtcagggg tccggtcgcg accagatgga tgctgccggg
     3841 cgccgccctg agagtgtcga tgatgaagtc gacggcgtgg tcgtcgtggg cctccaacac
     3901 cgcctccggc agcacgacgt cgccgagtcc gcccgcccca tgcacgtggg cggcatcgcg
     3961 cacctgccgc gtcaggggct cggccgcacc cgcggcgacg ggaaccttgt caaacccgag
     4021 gtactcgcgc agctggagag cattgcgcgt ggtcgtctcc agaccgacgt tgccgccgac
     4081 cgtagtgacc cccacaaggt tcaggttcgg gtcgccgtgg gcgagcagaa gagcgagggc
     4141 gtcatccagc ccggggtcgc agtcgatcaa gatggagcgg gccatgactc gagcgtacgc
     4201 cgcgtggcag gcggcgtaga atctcgtgtg ccctccatcg accccgccga actcgcgacc
     4261 accctgaagg tgctcgagca gatggccgag ctcgatgagg aggagccgga cttcgtgacc
     4321 gtgcggcgcg ccacggcccg catgttcaag gcggtcaaga agacgcgccg actcgagaag
     4381 cgggcggaaa tcgccgagtc cgaccgcagc gtgatcgccg ccacggccac cggcgcaccg
     4441 gaccggatcg atgacgagac ccggggcatc ccgatctcga cgagcacgac ggcaccgacc
     4501 gcgggcaccc tgatcaaggc ccgcgcctgc tacatctgca agcagccgta cacactggtc
     4561 gatgccttct accaccagct ctgcccggac tgcgcggccc tcagccacgc gaagcgggac
     4621 gcccgcaccg acctcaccgg ccggcgtgcg ctgctcaccg gtggtcgcgc caagatcggc
     4681 atgtacatcg cgctgcgtct gctgcgcgac ggcgcccaca ccacgatcac gacgcgcttc
     4741 ccgcgggatg ccgtgcgccg cttcaccagc ctccccgact cgcccgagtg gatcgaccga
     4801 ctgcgcatcg tgggaatcga cctgcgcgac cccgcccagg tcatcggtct cgccgactcg
     4861 gtcgccgctg ccgggccgct cgacatcctc atcaacaatg cgacccagac ggtgcgccgc
     4921 tcgcccggcg cctatcagcc cctggtggat gccgaactcg cccccctgcc cgacggaccg
     4981 cttcccgagc tggtcacctt cggccacacg aacgatcaac acccgctcgc cctcgagcgc
     5041 agcctggccg cgcacccgat cctcgcggcg gcggccgatg tgagcgcggc cgcacaggag
     5101 ctcgccgacc aggcgatggg cgctggcaat tcgtccctcg aacgactcgc cgccggcacg
     5161 gcgatcgacg cgggcgggct gctgcccgac ctcgacgacg tcaactcgtg gacgcaggcc
     5221 gtgcacgagg tcgacccgct cgagatgctc gaggtgcagt tggccaacac gacggctccc
     5281 ttcatcctga tctcgcggct gcgcccgtcg atggccgcgg ccgccgcgcg gcgcaagtac
     5341 gtggtgaacg tgagcgccat ggagggggtg ttcgggcgcg ggtacaaggg gccggggcat
     5401 ccgcacacca acatggccaa ggccgccgtg aacatgctca cgcgcacgag cgcgcgggag
     5461 atgttcgaga ctgacggcat cctgatgacg agcgtcgaca ccggctggat caccgacgaa
     5521 cgaccgcacc ccaccaaggt gcggctggcc gaggagggct tccacgcacc gctcgacctg
     5581 gtggacggcg ccgcgcgcgt ctacgacccg atcgtgcgcg gcgaggcggg cgaggatgtc
     5641 ttcggcgtct tcctcaagga ttacgctccg agtagttggt gagcgcagcg ctggtgaacc
     5701 tcgaacccgg catgcgggtc gtgatccggt accgcctgga cgacggccgg gcaacggatg
     5761 cgctcggcat ccttctctcc ctgaccagca gtcatgccgt cgtcgacacc aagcgcggcg
     5821 cggagtcgat cgagctggtt cgggtcatcg catcgaaaga ggttccccct cccccacgac
     5881 cccgcccatg aggtaggaat gggggtagac agatcggatc ccccatggcg acacttccgg
     5941 agtcatcccg gtggaagcgc aagcccgtcg atcactgcga cgtggtgatg aagggtggga
     6001 tcacgtcggg ggtgatctac cctcgagcgc tcgttgagct ggcccgccat taccggttcc
     6061 gtggcatggg cggttcgtcc gcgggcgcca tcggtgcggc gctgggcgcc gccgctgaat
     6121 tcgggcggtc acgcggtggc ttcgcccggc tctacgagct gccggaccaa ctggacctgg
     6181 gctcgctctt ccggccggag cgctcgaccc gtgccctgct gcccatcctc atggccttca
     6241 caggattccg gcccgacggc ggtcagcgca ccgggttcgc ccgagcgctg gcgatcgtcg
     6301 gcgcgatagc cagcggtttc ccgctcgccg gcatcctggg tctgctgccc ggcgtggggc
     6361 tgatcatcgc cggcgctctg gccgactcgt ggcccgggta ccttctcatc ccctccgggg
     6421 cggtgctcgc catcatcggc tggatcgtcg ccgtgggcat tcgactgggc tggaagctca
     6481 cgcgcagcgt tcccgcgaac ctgttcggca tctgccgggg actggatccc accggtggcg
     6541 acgacggcct caccaattgg ctctcgacga agctcgatct gatcgcaggt ctcaccgccg
     6601 aggaggggcc actgcgcttc ggccacctgt ggaccggtcg acggaacttc cgcgccgggt
     6661 cgatcggcga catccccgca ttcgagcgca gcatcgacct gcgcatgatc agcacctgcc
     6721 tgagccggtc acggccctac gagatgccgc tcaaggcacg caccttcctg tacgaccctg
     6781 cggtctgggc gaccct
//
LOCUS       DAOSOU010000103         6780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_11299_length_6780_cov_5.341413, whole genome shotgun sequence.
ACCESSION   DAOSOU010000103 DAOSOU010000000
VERSION     DAOSOU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11299_length_6780_cov_5.341413"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..157
                     /locus_tag="PLW15_06850"
                     /pseudo
     CDS             44..157
                     /locus_tag="PLW15_06850"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            206..409
                     /locus_tag="PLW15_06855"
     CDS             206..409
                     /locus_tag="PLW15_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOT34147.1"
                     /translation="MTAQGKVNINTADASALETLPRVGPAMAARIIDWREVNGRFAAI
                     EDLMSVTGIGQKTFDGLKDLVTV"
     gene            406..2646
                     /locus_tag="PLW15_06860"
     CDS             406..2646
                     /locus_tag="PLW15_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOT34148.1"
                     /translation="MIADLRLAIPAAVAWIAAAVIIGIDPVIPTIALWAAAGIATAIA
                     LARRSLAVVALALAAAAFCSTSVVVQSTSRQPAELVAAAETGRQIDALATLTGRASGR
                     SYPVTLDVVNGTSVHVPVVVFGPATDAGIGTTVRITGTLTATDPGDDAAFLVFAERPP
                     VVESPPPWYLDWANGLRARFLAQARGLPGDGGDLLAGLAIGDTTDVGGTLDQAMKSSS
                     LSHLTAVSGANCAIVIGLVMVAGAAVGIPRWARVAASLAVLVGFVVLVTPEPSVLRAA
                     VMASLVLATLARGRPVRGIPVLALATICLLAFDPWLARSYGFVLSVLATAGLLLLAGP
                     LARVLERWLPRWLAIVIAVPFAAQLACQPVIILLSATLPTYGVVANVLAAPAAPIATV
                     VGLAACVLLVLIPPLGALLCQLAWLPSAWIAAVAQFFASAPAAQLPWPPGAVGALLLA
                     SGTVLAVFAVLGRRWAVVALAVAGAVYLGLAGGGRIAQVTGRPDWQIAACDIGQGDAV
                     FVRSRGELALVDTGPDPALMRRCMTELGIGRVDLLVLTHYDLDHVGGAGALVGKVDRV
                     FVGPSSDPEDDRLRADLVAGGAHVEQVSRGPTGLLGDLRWEVLWPPSRLGSIEPGNPA
                     SVTLEFQPVGECASGCLSSIFLGDLGEDAQNRLLAAGPVGTVDVVKVAHHGSRDQSER
                     LYERLHATVGVIGVGADNGYGHPTDDLLQILARAGTIPERTDLSGLILLAPGAEPGTV
                     RVWSER"
     gene            2657..3661
                     /gene="holA"
                     /locus_tag="PLW15_06865"
     CDS             2657..3661
                     /gene="holA"
                     /locus_tag="PLW15_06865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT34149.1"
                     /translation="MAAPKTVIPVLQWHSVRPAPVVLVSGPEEYLADRAIRSLRELLK
                     AEDPSLEIHDVEADQYGPGELLTLASPSLFNEPRLIRVVKVEKCTDAFITETLDYLSH
                     PADDTYLVLRHASGVRGKKLLDAIRAGTGGGIEISCLELKKDTEKTEFAAAEFAAAGR
                     RVTGGAIRALVSAFSDDLAELAAACQQLLADASEEITEVTVEKYYSGRMETNAFKVAD
                     AAIAGRRGEALVLLRHALSSGADPVPIVAAFAAKLRTMARISGSNAPAGQLASKFGLA
                     PWQVDRARRDLHGWTDEGLGRSLETLADTDEQIKGLGRDPVFALERMIGVIASRGGSA
                     "
     gene            complement(3708..3968)
                     /gene="rpsT"
                     /locus_tag="PLW15_06870"
     CDS             complement(3708..3968)
                     /gene="rpsT"
                     /locus_tag="PLW15_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT34150.1"
                     /translation="MANIKSQIKRIGTNKKAQERNKAVKSEVKTAVRATREAITAGDK
                     EKATASLALAGKKLDKAASKGVIHKNQAANRKSAIAKQVAAL"
     gene            complement(4054..4764)
                     /locus_tag="PLW15_06875"
     CDS             complement(4054..4764)
                     /locus_tag="PLW15_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT34151.1"
                     /translation="MPSSVVLVHGLRTSATMWRTQVAALEAAGHEAIAIDLPGHGARM
                     GERFTVAEAVEAIGDAVGAARHPVLLCGFSLGGYMSIHYAGLAERPIVGLLAASCGTR
                     PYRVVLDTWRAAARVIHRFPDRGLGLNNWAVRLAVRDPELTTDVIAGGVALEVMDDAL
                     VELRQLHPVTSLSRIAQPIWLVNGTLDHFRLEERRYLAAAPDARLIHVPGATHMVSLT
                     RPREFTRILLDAVDSTAA"
     gene            4790..5941
                     /locus_tag="PLW15_06880"
     CDS             4790..5941
                     /locus_tag="PLW15_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012244105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT34152.1"
                     /translation="MPSLAPHILGVPGSGIRRIHEIAITLDDVIMLAVGEPDVPVAPH
                     IAAAAKAAWDADETNYTANAGILPLRQAIVDKLARVNDLHVTTDQVWVTVGATQGLHQ
                     AMALTLGPGDEVLLPDPGYTTFTMNAHMLDAVPVPYPLHPESGFMPDVATLESLVTPR
                     TRVLILNSPSNPLGTIFPESVLTELLDFARRHDLWVISDEVYEYFTWGEPHVSTAALD
                     TDGRVLSVFSLSKTYAMTGIRIGYLVTPPGMVDTMRAVQEATISCASAPGQHAAIAAI
                     TGDQSHVAEAREHYRGNLAAATELLASRGIRYLNPGGAFYLWIDVSHASGGNVADWAE
                     NFLRVSGVAVAPGSAFGASGEGWIRVCLAAKRADVLEGLGRLPVPQSGG"
     gene            5982..6302
                     /locus_tag="PLW15_06885"
     CDS             5982..6302
                     /locus_tag="PLW15_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOT34153.1"
                     /translation="MTDTATLPTTPTSTTTVNAATDSQVLSILSLISALSGILFGLVF
                     PLSIVAIVLGILALSREPRGRTMAIWGIVVGAVPGALAVLGIVFAAAFLIPFGVFAAA
                     FGGF"
     gene            6402..>6780
                     /locus_tag="PLW15_06890"
     CDS             6402..>6780
                     /locus_tag="PLW15_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT34154.1"
                     /translation="MSPRATTALEPASTDPAFIRNFCIIAHIDHGKSTLADRMLGITG
                     VVSDRDMRAQYLDRMDIERERGITIKSQAVRMPWATGAGGPGETYALNMIDTPGHVDF
                     TYEVSRSLAACEGAVLLVDAAQGI"
BASE COUNT          961 a         2404 c         2285 g         1130 t
ORIGIN      
        1 ccggcggcag cgacagcgtc gatgacgcga tcgcccgggg ctctacggtg tgcgtgaggg
       61 cgatcgcgtc atcgacgctg tcgctgccgc cggcggattc accgactcgg ccgaccagac
      121 gcaggtgaat ctggcacggt ttctcgttga cggggagcag ctctacgtgc cgacggtggg
      181 ggagacgcct gcgtcggcac ccggggtgac ggcccagggc aaggtcaaca tcaataccgc
      241 cgatgcgtca gcgctcgaga cgttgccgcg ggtcggcccc gcgatggccg cccgcatcat
      301 cgactggcgt gaggttaacg gacgcttcgc ggcgatcgag gatctcatga gcgtcaccgg
      361 catcggtcag aagacgttcg acggcctcaa ggatctcgtg acagtgtgat cgccgacctg
      421 cgcctcgcca tcccagcagc tgtggcctgg atcgcggcag cggtgatcat cggcattgat
      481 ccggtcatcc cgacgatcgc gctctgggca gctgccggca tcgccaccgc catcgcgctc
      541 gcccgacgct cgctcgcggt cgtcgcactc gcgctggcgg ccgcggcgtt ctgttcgacg
      601 tccgtcgtgg tgcagtcgac gtcgcggcaa ccggccgagc tggtggcagc ggccgagacc
      661 ggtcgccaga tcgacgccct cgcgactctc accgggcgcg cgagcgggcg ctcctatccg
      721 gtcactctgg atgtcgtcaa cggcacttca gtgcacgttc cggtggtcgt gttcgggccc
      781 gcgaccgacg ccggtatcgg cacgacggtg cggatcaccg gaacgctcac cgcaaccgac
      841 cccggcgacg acgcggcctt cctggtattc gccgagcggc cgcctgtcgt cgagtcaccg
      901 ccgccgtggt acctcgactg ggcgaacggg ttgcgggctc ggttcctcgc gcaggctcga
      961 ggtcttccgg gtgacggggg agatctcctc gcggggcttg ccatcggcga taccaccgac
     1021 gtcggcggca cgctggatca ggcgatgaag tcgagctcgc tgagtcacct gacggcggtg
     1081 tccggcgcca actgcgcgat cgtcatcggg ctcgtgatgg tcgcgggggc tgcggtcggc
     1141 ataccgcgct gggcccgggt cgcggcatcc ctcgctgttc tggtggggtt cgtcgtgctc
     1201 gtcacgcccg agccgagtgt ccttcgggcg gccgtgatgg cgagcctcgt gctggcgacc
     1261 ttggcacggg ggcgtcccgt tcgcggcatc cccgtgctcg ccctcgcgac catctgcctg
     1321 ctcgcgttcg acccctggct cgctcgtagc tacgggttcg tgctgtcggt gctcgcaacg
     1381 gcggggctgc ttcttctcgc gggtccgctg gcgcgggtgc tcgagcgctg gctcccgcgc
     1441 tggctcgcga tcgtgatcgc ggttccgttc gccgcacaac tcgcctgcca accggtcatc
     1501 atcctgctgt ctgcaaccct tccgacctac ggcgtggttg ccaatgtgct cgccgctccg
     1561 gctgcgccga tcgcgaccgt ggtcgggctc gccgcctgcg tgctgctggt gctgattccg
     1621 ccgctcggtg cgttgctctg tcagttggcg tggctgccgt cggcatggat cgcggctgtc
     1681 gcccagttct tcgcgtcggc accggcggct cagctgccct ggccgccggg cgcagtcggt
     1741 gcactgctgc tcgcctcggg gacggtgctc gcggtcttcg ctgtgctggg ccggcggtgg
     1801 gcggtcgtcg ctctggccgt cgcgggcgcc gtctatctcg ggctcgccgg cggcggtcgc
     1861 atcgcgcagg tgaccggccg ccccgactgg cagatcgcgg cgtgcgacat cgggcagggc
     1921 gacgccgtct tcgtgcgcag ccgcggcgag ctcgcgctcg tcgacaccgg ccccgacccg
     1981 gcgctcatga ggcggtgcat gaccgagttg ggcatcgggc gcgtcgatct gctggtgctc
     2041 acgcactacg acctcgacca cgtcggcggc gccggcgctc tggtgggcaa ggtcgatcgg
     2101 gttttcgtcg gcccgagcag cgatcccgaa gacgaccggc tgcgggccga cctcgttgcg
     2161 ggcggtgctc acgtcgagca ggtgtcccgg gggcccacgg gactgctggg tgacctgcgg
     2221 tgggaggtcc tctggccgcc atcgcgtctg ggcagcatcg agcccggcaa tcccgcgagc
     2281 gtgacgctcg agttccagcc ggtgggcgag tgcgcctccg gatgcctcag ctccatcttc
     2341 ctcggagacc tcggcgaaga cgcccagaac cgtctcctcg ccgctggacc cgtcggcaca
     2401 gtcgacgtcg tgaaggtggc gcaccacggc tcgcgtgacc agagcgagcg gctctacgag
     2461 cgtctgcacg cgactgtcgg cgtgatcggg gtgggcgcag acaacggcta cgggcatccc
     2521 accgatgatc tcctgcagat tctcgcccgg gccggaacca tcccggagcg taccgacctc
     2581 agcgggctga tcctgctcgc gcccggcgcg gagcccggca cggtgcgtgt ctggagcgaa
     2641 cgttaggctg tgatccgtgg ccgcccccaa gaccgtgatt cccgtgctcc agtggcactc
     2701 ggtgcgacca gcgccggtcg tgctggtgtc gggtcccgag gagtacttgg ccgatcgcgc
     2761 catccgctcg ctgcgcgaac tgctcaaggc cgaagacccg agcctggaga tccacgacgt
     2821 cgaggccgac cagtacggcc cgggggagct tctcaccctc gccagcccgt cgctgttcaa
     2881 cgagccgcgc ctcatccgcg tcgtcaaggt ggagaagtgc accgacgcct tcatcaccga
     2941 gaccctcgac tacctctcgc accccgccga tgacacctac ctggtgctgc ggcacgcgag
     3001 cggggtgcgc ggcaagaagc tcctcgacgc cattcgcgcg ggtaccggcg gcggcatcga
     3061 gatctcctgt ctcgaactca agaaagacac cgagaagacc gagttcgcgg cggccgagtt
     3121 cgccgcggcc ggccgccggg tcaccggggg agccatccgt gccctcgtct cggcgttctc
     3181 cgacgatctg gccgaactcg ctgccgcgtg ccagcagttg ctcgccgacg cgagcgagga
     3241 gatcaccgaa gtcacggtcg agaagtacta ctccggccgc atggagacca acgcgttcaa
     3301 ggtcgcggat gccgcgatcg ccggccgccg cggagaagcc ctcgttctgc tgcgccacgc
     3361 cctctcctca ggtgcagacc cggttcccat cgtggccgcc ttcgccgcca agttgcgcac
     3421 gatggcgcgc atctccggtt ccaacgcgcc ggccggtcag ctcgcgtcga agttcggcct
     3481 cgcgccctgg caggtcgacc gcgcgcggcg agacctgcac ggctggactg acgaggggct
     3541 cggtcgatct ctggagaccc tcgccgacac cgacgagcag atcaagggct tgggccgcga
     3601 cccggtcttc gccctcgagc gcatgatcgg cgtcatcgcg agccgcggcg gcagcgctta
     3661 acgagaaatg ccccggccga ggccggggca tttctgaaaa cagctgacta cagcgcagca
     3721 acctgcttgg cgatggccga cttgcggttc gctgcctggt tcttgtggat gacacccttg
     3781 ctggcggcct tgtcgagctt cttgccggcc agggcgagcg acgcggtcgc cttctccttg
     3841 tcaccggcgg tgatcgcctc gcgggtcgcg cgaacggcgg tcttgacctc gctcttgacg
     3901 gccttgttgc gctcctgcgc cttcttgttg gtgccgatgc gcttgatctg cgacttgatg
     3961 tttgccacgt gggtgctttc tgttggtgtg aaaggagtta tctcgcgagc ctctggagcg
     4021 aaactacgcg gaagccaaag gtcaacatta ccatcaggcg gcggtcgagt ccaccgcgtc
     4081 gagcaggatg cgcgtgaact cccgcgggcg ggtcagggag accatgtggg tggcgcccgg
     4141 cacgtggatg agccgggcat ccggtgccgc agcgaggtag cggcgctcct cgagacggaa
     4201 gtggtcgagg gtcccgttga ccagccagat cggttgggcg atgcggctca gtgaggtcac
     4261 cggatgcagc tgccgcagtt caacgagcgc gtcatccatc acctccagcg ccaccccgcc
     4321 ggcgatcacg tcggtcgtga gttcgggatc gcgcaccgcc agccgcacag cccagttgtt
     4381 cagcccgagc ccgcggtcgg gaaaccgatg gatcacccgc gccgcggccc gccacgtgtc
     4441 gagcaccaca cggtagggcc gagtgccgca gctggccgcg agcagaccca cgatcggccg
     4501 ctcggccagc cccgcgtagt ggatcgacat gtacccgccg agcgagaacc cgcacagcag
     4561 caccggatgc cgcgccgctc ccaccgcgtc gccaatcgcc tcgacggcct ccgccaccgt
     4621 gaaccgctcg cccatgcgtg cgccgtgccc cggcaggtcg atagcgatgg cttcgtgccc
     4681 agcggcctcg agcgccgcga cctgtgtgcg ccacatggtc gcggaggtgc gcagtccgtg
     4741 cacgagcacc accgagctgg gcatgctccg atggtaatag cctgaagcca tgccctccct
     4801 ggccccgcac atcctcggcg ttcccggcag tggaatccgt cgcatccacg agatcgcgat
     4861 cacgctcgac gacgtgatca tgctcgcggt gggggagccc gacgtgccgg tcgcgccgca
     4921 catcgccgct gcggcgaagg cagcctggga tgccgacgag acgaactaca cggccaatgc
     4981 gggcatcctg ccgctgcgtc aggcgatcgt cgacaagctc gcgcgggtca acgatctgca
     5041 tgtgaccacc gaccaggtct gggtgacggt cggggcgacc caaggcctgc accaggccat
     5101 ggcgctgacc ctcgggccgg gcgacgaggt gctgctgccc gatcccggct acacgacctt
     5161 cacgatgaac gcccacatgc tcgacgccgt gccggtgccc taccccctgc acccggagtc
     5221 gggcttcatg ccggatgtcg ccaccctcga gtcgctcgtc acccctcgca ctcgcgtgct
     5281 catcctcaat tcgccctcga acccgctggg cacgatcttc cccgagtccg tgctcacgga
     5341 gctgctcgac ttcgcccgcc gccacgacct ctgggtgatc agcgacgagg tgtacgagta
     5401 cttcacctgg ggagaacccc acgtgagcac ggccgcgctc gacaccgacg gccgggtgct
     5461 cagcgtgttc tcgctctcga agacctacgc gatgacggga atccgcatcg gctacctcgt
     5521 caccccgccg ggcatggtcg acaccatgcg cgcggtgcag gaggcgacga tcagctgcgc
     5581 cagcgctccg ggccagcacg cggccatcgc cgcgatcacg ggggatcaga gccatgtggc
     5641 cgaggcgcgg gagcactacc ggggcaatct cgccgcggcc accgagctgt tggcgagccg
     5701 gggcatccgc tatctgaatc cgggcggcgc cttctacctg tggatcgacg tcagccacgc
     5761 gtcgggcggc aacgtcgccg actgggcgga gaacttcctg cgcgtcagcg gagtcgccgt
     5821 cgcgccgggc agcgcgttcg gtgcgtcggg ggagggctgg atccgggtgt gtctcgccgc
     5881 gaagcgcgcc gacgtgctcg agggcctcgg gaggctgccc gtgccgcaat ccgggggtta
     5941 actccatacc ggcaggcgcc tcagaacgcg aggctggatg catgactgat acagctacgc
     6001 tccccaccac cccgaccagc accaccaccg tgaatgcggc caccgacagc caggtgctca
     6061 gcatcctgag cctgatctcg gcgctgtccg gaatcctctt cggcctggtg ttccccctct
     6121 cgatcgtcgc gatcgtgctc ggaattctcg cgctctcgcg tgagccgcgc ggtcgcacca
     6181 tggccatctg gggcatcgtc gtcggcgccg tccccggtgc cctcgcggtg ctcggcatcg
     6241 tgttcgctgc tgcgttcctg atcccgttcg gcgtgttcgc cgcggccttc ggcggtttct
     6301 gatcgaagcg gatgcccgtg gcacagcacc acgggcatcc acccccgcgc gcgtctcgta
     6361 aactggtgcg cggccctcag ggccactacg agaggcgcac gttgagtccc agagccacca
     6421 cggcactaga acctgcctcg accgaccccg cgttcatccg caatttctgc atcatcgcgc
     6481 acatcgacca cggcaagtcg acgctcgccg accgcatgct cggcatcacc ggcgtcgtct
     6541 ccgatcgcga catgcgcgcc cagtacctcg accgcatgga catcgagcgc gagcgcggca
     6601 tcaccatcaa gagccaggcc gtgcgcatgc cgtgggccac gggtgctggt ggcccgggag
     6661 agacctacgc gctcaacatg atcgacacgc ccggtcacgt cgacttcacc tacgaagtat
     6721 cgcgctcgct cgccgcgtgc gagggcgccg tgctacttgt cgacgccgcg caggggatcg
//
LOCUS       DAOSOU010000104         6730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_11416_length_6730_cov_4.786966, whole genome shotgun sequence.
ACCESSION   DAOSOU010000104 DAOSOU010000000
VERSION     DAOSOU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11416_length_6730_cov_4.786966"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PLW15_06895"
     CDS             <1..1069
                     /locus_tag="PLW15_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOT34155.1"
                     /translation="EIGREINLGSPKQLQEVLFDQLGMPKTRSNKTGFSTDAASLADL
                     QELNPHPFLDLLLQHRDATKLMQIIASIDKAVDAGGRVHTTYEQAGSSTGRIASNDPN
                     LQNVPVKTEIGREIRSAFEAGEGFETLLTADYSQIEMRIMAHLSGDEGLISAFNEGED
                     LHRFVGARIFGVAPADVTPTMRTKVKAMSYGLAYGLSAFGLSKQLRIETSEAKELMAD
                     YFARFGAVREYLRNVVEQAKVDGYTTTIFGRRRPFGDLSSSNRVLRENAARQALNSPI
                     QGTAADILKRAMIEIDRDMRGMSSRMLLQVHDELVFEVAPGELDDLSSIVRTRMAGAA
                     ELRVPLDVQIGTGPNWDAAAH"
     gene            1145..1414
                     /locus_tag="PLW15_06900"
     CDS             1145..1414
                     /locus_tag="PLW15_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOT34156.1"
                     /translation="MTERTVTVASSVGLHARPASLFAQAAAKAGVLITLTSAAGKSVN
                     AASILGVLSLGISHGETVTLSADGDGADAALDTLVEVLSMDLDKQ"
     gene            1438..3117
                     /locus_tag="PLW15_06905"
     CDS             1438..3117
                     /locus_tag="PLW15_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOT34157.1"
                     /translation="MSTDDARTPTEIDQIAEDWVTTLAELDPAVATWIGIPGKLDEYE
                     DLSPEGHAKVISAARGVLAKLESAAVVDDVDWVTKTDLTNELTLDLEADAAGLWMRDV
                     NVIASPAQGIRDILDLMPTETEGNWADIAGRLSNLPKAVDGYIETLRLGIANGVTPAK
                     RQVREVIDQARKHGAGDGFFHNFTGAASLADDQPVPESLKADLAKGAVVSAAAYEKLA
                     AFLTDELLPAAQEQDGVGREIYALQSRRFLGAKIDLDETYEWGIEELARMAAEQEAIA
                     NEIKPGASVAEAIEILDGDPTRKLHGTKALQEWMQQLSDRAVSELSASHFDIPDPIKR
                     LECMIAPTQEGGIYYTSPSDDFSRPGRMWWSVPEGVTEFNTWRETTTVYHEGVPGHHL
                     QLGQAVYNRAKLNTYRRQLAGTSGHAEGWALYAERLMEELGYLDDPGDRLGMLDGQRM
                     RAARVVLDIGVHLGKPKLDGTGQWDWDYALDFMSKNVNMNAQFVRFEVNRYFGWPGQA
                     PSYKVGQRIWEQIRDAYKAAEGESFDIKEFHRKALDVGGVGLDTLRAALLK"
     gene            3114..3749
                     /locus_tag="PLW15_06910"
     CDS             3114..3749
                     /locus_tag="PLW15_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOT34158.1"
                     /translation="MSHLVEAPTIGRAWLEIAAHIMAQGSPATYDGRPILEVAQVTLE
                     VAHPDPDDELIARNADPERLAWMRANFLDRSLVAELGDARSYASRLRDYGGVGRDQVE
                     WVIARLRADPQSRSATITTFEPLIDTTYIPCVSMLDFWVPADSLELVVYAHSIDFGSK
                     GYGNLVQLAAIQQEVASALGLDVGSLTFVIKSAHVYDTEFDYMRGVLAHSS"
     gene            3827..4771
                     /locus_tag="PLW15_06915"
     CDS             3827..4771
                     /locus_tag="PLW15_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005504590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOT34159.1"
                     /translation="MAEKLTRKRAIVAAALSLALVLPAGAGAAWALDRFVIQHVEITD
                     VAAYEAENSTVATVDATSEAVTTATTYQSDTASISISTVVTGSGTDTVTYYVADVQLT
                     DATALRSAFAQNSFGENIVENPSAIAEDNGAVFAINGDYYGFRDSGIIIRNGVIYRDS
                     GARTGLAFYLDGHVEVYDETATTAQELLDSGVWNTLSFGPAIVENGETVDGIESVEVD
                     TNFGNHSIQGDQPRTAVGVIDDNHLVFVVVDGRSPGYSKGVTLTGLADIMLGLGATTA
                     YNLDGGGSSTMYFNGSLVNNPLGTGTERGTSDILYIGG"
     gene            4775..5845
                     /locus_tag="PLW15_06920"
     CDS             4775..5845
                     /locus_tag="PLW15_06920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34160.1"
                     /translation="MIVLIPAYEPGGTLSQLVRDLRASESEPHVVVVDDGSGAAYAEE
                     FDAARRAGASVISYSVNRGKGFALRTGIAHIVERFPGEVVVCADSDGQHRVDDILRVA
                     HRAEEAEGAIILGGRRFIGDVPVRSRLGNAVSRLAFSAVAGVRVHDTQTGLRGYPPHL
                     LGWMLSIAGDRFEYELNLLLDAAATGIPVIEVEVATVYLRNNASSHFRPVVDSVRVLR
                     PLMAFGVSSFGAFLLDLAALQVIFALTGSLLIAVVGARAVSGTVNFLVNRYLVFRSRA
                     TTVRSHALRYLALALAIVVAGYLMLTALSALGVGLVPAKIVTDVTLYVLSFQAQRLVI
                     FARRATAPVAKNRELVLSSRRR"
     gene            5964..>6730
                     /locus_tag="PLW15_06925"
     CDS             5964..>6730
                     /locus_tag="PLW15_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT34161.1"
                     /translation="MTIVTTDKAPKQVAVNDIGSAEDFLAAVEKTLKFFNDGDLIEGT
                     VVKIDRDEVLLDVGYKTEGVIPSRELSIKHDVDPTEVVKVGDLVEALVLQKEDKEGRL
                     ILSKKRAQYERAWGDVEKIKDADGIVTGSVIEVVKGGLIVDIGLRGFLPASLIELRRV
                     RDLTPYLGQEIEAKILELDKNRNNVVLSRRALLEQTQSESRTTFLNNLAKGQVRKGVV
                     SSIVNFGAFVDLGGVDGLVHVSELSWKHIEHASEVVEV"
BASE COUNT         1229 a         2231 c         2152 g         1118 t
ORIGIN      
        1 cgagatcggc cgcgagatca acctgggcag ccccaagcaa ctccaggagg tgctgttcga
       61 ccagttgggc atgcccaaga cccgatcgaa caagaccggg ttctcgacgg atgccgcgag
      121 cctcgccgac ctgcaggagc tcaacccgca ccccttcctc gacctcctgc tgcagcaccg
      181 cgacgccacc aagctgatgc agatcatcgc gtccatcgac aaggccgtgg atgccggcgg
      241 ccgggtgcac accacctacg agcaggcggg ttcgagcacc ggccgcatcg cctccaatga
      301 cccgaacctg cagaacgtgc cggtcaagac cgagatcggg cgcgagatcc ggtcggcctt
      361 cgaagccggc gagggcttcg aaaccctcct gaccgctgac tactcccaga tcgagatgcg
      421 catcatggcg cacctctcag gcgacgaggg gctgatctcg gccttcaacg agggcgagga
      481 cctgcatcgc ttcgttggtg cgcgcatttt cggagtggcg ccggcagatg tgacccccac
      541 catgcgcacg aaggtcaagg cgatgtcgta cggtctcgcc tacggactca gcgcgttcgg
      601 actcagcaag cagctgcgca tcgagaccag cgaggccaag gagctcatgg cggactactt
      661 cgctcgcttc ggtgccgtgc gggagtacct gcgcaacgtc gtcgagcagg cgaaggttga
      721 cggatacacg acgacgatct tcggtcgccg ccgtccgttc ggcgacctgt cgtcgtcgaa
      781 ccgggtcctg cgcgagaatg cggcgcggca ggcgctcaac tcgccgatcc agggcactgc
      841 ggcggacatc ctcaagcggg cgatgatcga gatcgatcgg gacatgcggg gcatgagctc
      901 ccgcatgctc ctccaggtcc acgacgagtt ggtcttcgag gtggcgccgg gggagctcga
      961 cgacctcagc tcgatcgtgc gcactcgaat ggcgggcgca gcggagttgc gggttccgct
     1021 cgacgttcag atcggcaccg gcccgaactg ggatgccgcg gcccactgag cccgccgctc
     1081 tgcacggcaa ggggcgccaa tcaccgtcac cattgagcag tagcgccaac cttcaaggag
     1141 agtcatgacc gagagaacag tcaccgtcgc atccagtgtc ggattgcacg cccgtccagc
     1201 atccctcttc gcccaggccg cggccaaggc gggcgttctc atcacactga cctccgccgc
     1261 aggcaagagc gtcaacgcgg ccagcatcct cggagtgctc tccctgggca tcagtcacgg
     1321 tgagacggtc accctcagcg ccgacggtga tggcgctgac gccgcgctcg acaccctcgt
     1381 cgaggtgttg tcgatggacc tagacaagca gtagacaccg tcgcatatgc tcgattcatg
     1441 agcaccgatg acgcgcgcac tcctaccgag atcgaccaga tcgccgagga ctgggtcacc
     1501 acactcgcag aactcgaccc cgcggtcgcc acgtggatcg gtattccggg caagctcgac
     1561 gagtacgagg atctgtcgcc ggagggccac gcgaaggtca tctcggctgc tcgcggagtg
     1621 ctcgccaagc tcgagagcgc ggcggtagtc gacgacgtcg actgggtcac caagacggat
     1681 ctgaccaatg aactgaccct cgatctcgaa gcggatgccg cgggcctgtg gatgcgcgac
     1741 gtcaacgtga tcgcctcccc ggcccagggc atccgggaca ttctcgacct gatgccgacg
     1801 gagaccgagg gcaactgggc ggatatcgcc ggccgtctct ccaatctgcc gaaggcagtg
     1861 gacggctaca tcgagacgct gcgcctcggc atcgccaacg gcgtgacgcc cgcgaagcgg
     1921 caggtgcgcg aggtgatcga tcaggctcgc aagcacggag cgggcgacgg tttcttccac
     1981 aacttcaccg gggctgcctc gctggccgac gatcagccgg ttcccgagtc cttgaaggct
     2041 gacctcgcga agggcgctgt ggtctctgcc gcggcatatg agaagctcgc ggccttcctc
     2101 accgacgaac tccttcccgc ggcgcaggaa caggacggcg tggggcgcga gatctacgcg
     2161 ctgcagtcac gacggttcct gggggcgaag atcgacctcg acgagaccta cgagtggggg
     2221 atcgaggagt tggcgcggat ggccgccgaa caggaggcca tcgccaacga gatcaagccc
     2281 ggggcatccg ttgccgaagc gatcgagatc ctcgacggtg acccgacgcg caagctgcac
     2341 ggcacgaagg cgcttcagga gtggatgcag cagctcagcg atcgcgctgt ctcggaactg
     2401 tcggcgagcc acttcgacat ccccgacccg atcaagcgac tcgagtgcat gatcgcgccc
     2461 acgcaggagg gtggcatcta ctacacgagc cccagcgatg acttctcgcg gccgggtcgc
     2521 atgtggtggt cggtgcccga gggtgtgacc gagttcaaca cctggcgcga gacgacgacc
     2581 gtctaccacg agggagttcc cggccaccac ctccagctcg gtcaggccgt ctacaaccgg
     2641 gccaagctca acacctaccg ccgccagctc gccggcacct cgggccacgc cgaggggtgg
     2701 gcactgtacg ccgagcggct catggaggaa ctcggctacc tcgacgatcc gggagaccgt
     2761 ctggggatgc tcgacggcca gcgcatgcgc gcggcccgcg tcgtgctcga catcggagtg
     2821 cacctcggca agccgaagct cgacggcact ggccagtggg actgggacta cgcgctcgac
     2881 ttcatgtcga agaacgtcaa catgaacgcc cagttcgtgc gattcgaggt caaccgctac
     2941 ttcggatggc ccggacaggc accgtcgtac aaggtggggc agcgcatctg ggagcagatc
     3001 cgcgatgcct acaaggctgc cgagggggag tccttcgaca tcaaggagtt ccaccgcaag
     3061 gctctcgacg tcggcggggt gggtctcgac actctcaggg cggccctgct gaagtgagtc
     3121 atctcgtcga ggcgccgacg atcggtcgcg cgtggctgga gatcgctgcg cacatcatgg
     3181 cgcagggcag ccctgccacc tatgacggtc gccccatcct cgaagtcgct caggtgactc
     3241 tcgaggtcgc ccatcccgac cccgacgacg agctgatcgc ccgcaatgcg gatcccgagc
     3301 ggttggcctg gatgcgtgcg aacttcctcg accgtagcct tgtggccgag ttgggcgacg
     3361 cccgcagcta cgcgagccgg ctccgcgact acggaggcgt cggacgtgac caggtggagt
     3421 gggtcatcgc gcgtctgcgc gccgacccgc agtcgcgatc agccaccatc acgaccttcg
     3481 aaccgctgat cgacaccacg tacattccgt gcgtgagcat gctcgacttc tgggtgccgg
     3541 ctgacagcct cgaactggtg gtctacgcgc acagcatcga cttcgggtcg aaggggtacg
     3601 gcaacctcgt gcaactcgcc gccatccagc aggaggtggc atcggcactc ggcctcgatg
     3661 tgggcagcct cacgttcgtc atcaagtcgg ctcacgtcta cgacaccgag ttcgactaca
     3721 tgcgcggcgt actggctcac tcttcatagc tcagccatag ggagcgcaca ggctccacgc
     3781 atgggcggct tgccgagcac atatcgtcgg cgcgtaccca tggagcatgg ctgagaaact
     3841 cacccgcaag cgcgccatag tcgccgctgc actctcactg gcactcgttc tcccggctgg
     3901 cgcgggggcc gcctgggcgc tcgaccgctt cgtcatccag cacgtcgaga tcacggatgt
     3961 ggcggcctac gaagccgaga actccaccgt cgccacggtc gacgcaacgt ccgaggcagt
     4021 gaccacggcg accacctatc agtccgatac cgcgagcatc tcgatctcga cggtggtgac
     4081 cggctccggc actgacacgg tcacctacta cgtcgccgac gtgcagctga cggatgccac
     4141 tgccttgcgc agcgcattcg cccagaacag cttcggcgag aacatcgtgg agaacccgtc
     4201 ggcgatagcc gaggacaacg gggccgtgtt cgccatcaac ggcgactact acgggttccg
     4261 cgacagcggg atcatcatcc gcaacggggt gatctatcgc gactccggtg cgaggaccgg
     4321 cctcgccttc tatctcgacg gccacgtcga ggtgtatgac gagaccgcca cgacggctca
     4381 ggagctgctc gactccgggg tgtggaacac tctctccttc gggccggcga tcgtcgagaa
     4441 cggcgagacg gtcgacggca tcgagtcggt cgaggtcgac accaacttcg gcaatcactc
     4501 gattcagggc gaccagccgc gcaccgcggt cggagtgatc gacgacaacc atctggtgtt
     4561 cgtggtggtc gacggtcgaa gccccggcta cagcaagggg gtgaccctca ccggcctcgc
     4621 agacatcatg ctcggcctcg gtgcgacgac cgcctacaac ctcgacggcg gcgggtcgtc
     4681 gacgatgtac ttcaacggtt cgctggtgaa caaccccctg ggaaccggca ccgagcgggg
     4741 cacctccgac atcctctaca tcggcggctg accgatgatc gtgctcatcc ccgcctacga
     4801 gcccggcgga acgctctcgc aactggtgcg tgatctgcgc gcatcagagt ccgagcccca
     4861 cgtggtagtg gtcgacgatg gcagcggcgc cgcctacgcg gaggagttcg acgcggctcg
     4921 gcgcgccggg gcatccgtga tctcctattc ggtgaaccga ggaaagggat tcgccctgcg
     4981 cacggggatt gcgcacatcg tggagcggtt ccccggggag gtggtggtct gcgccgacag
     5041 tgatggacaa caccgcgttg acgacattct gcgggtcgcc caccgcgccg aggaggcgga
     5101 gggcgcgatc atcctgggcg ggcgtcggtt catcggcgac gtgcccgtgc gcagcaggct
     5161 gggcaacgcc gtgtcgcggc tcgcgttcag tgcggtcgcc ggcgttcggg tgcacgacac
     5221 tcagacgggg ctgaggggat acccgccaca cctgctgggg tggatgctct ccatcgccgg
     5281 agaccggttc gagtacgaac tcaatctgct tctggatgcc gcggccacag gcatccccgt
     5341 gatcgaggtc gaggtggcca ccgtgtacct gcgcaacaac gcctcgtccc acttccgccc
     5401 cgtcgtcgac tcggtgcgcg tgctgcgacc gctgatggca ttcggtgtgt catcgttcgg
     5461 agcgttcctc ctcgatctcg ccgcgctgca ggtgatcttc gctctgacgg ggtcactgct
     5521 catcgccgtc gtgggggcca gggcggtgag cggcacggtc aatttcctcg tcaaccgcta
     5581 cctcgtcttc cgatcgcggg cgacgacggt ccggtcgcat gcgctgcgct atctggccct
     5641 ggccctggcg atcgtggtcg ccggatacct gatgctgacc gcactgtccg ccctgggagt
     5701 ggggctcgta ccagccaaga tcgtcaccga cgtgacgttg tacgtgctga gcttccaggc
     5761 gcagcggctg gtcatcttcg cgcgacgggc aactgcacct gtagcgaaga atcgcgaact
     5821 ggtactcagc tctcgcaggc gctaagctgg aatgtcgcat ttgtgacgtc tattcgcctg
     5881 cctctggcga gattcgaacc agaatcacca cccagcacca cccgccagtg gcccaaccct
     5941 gtccctatcg gagcaactac tacatgacaa tcgtaacgac cgacaaggcg cccaagcagg
     6001 tcgccgtcaa tgacatcgga tctgctgaag actttctcgc cgcggtcgaa aagacgctga
     6061 agttcttcaa cgacggagac ctcatcgagg gcaccgtcgt caagatcgac cgtgacgagg
     6121 ttctcctcga cgtcggttac aagaccgagg gtgtcatccc ctctcgcgaa ctttccatca
     6181 agcacgacgt tgaccccacc gaggtcgtca aggtcggcga cctggtcgag gctctcgttc
     6241 tccagaagga ggacaaggag ggtcgcctca tcctgtccaa gaagcgcgca cagtacgagc
     6301 gtgcatgggg cgatgtggag aagatcaagg acgccgatgg catcgtgacc ggttcggtca
     6361 tcgaggtcgt caagggtggt ctcatcgtcg acatcggcct gcgtggcttc ctgcccgcgt
     6421 cgctcatcga gctgcgccgc gttcgcgacc tcacgccgta cctcggccag gagatcgagg
     6481 cgaagatcct cgagctcgac aagaaccgca acaacgtcgt tctgtcgcgc cgcgccctcc
     6541 tcgagcagac ccagagcgag agccgcacga ccttcctcaa caacctcgcc aagggccagg
     6601 ttcgcaaggg tgtcgtgtcg tcgatcgtca acttcggtgc gttcgtcgac ctcggcggtg
     6661 tggacggtct cgttcacgtc tccgagctgt cgtggaagca catcgagcac gcctccgagg
     6721 tcgtcgaggt
//
LOCUS       DAOSOU010000105         6668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_11560_length_6668_cov_5.899743, whole genome shotgun sequence.
ACCESSION   DAOSOU010000105 DAOSOU010000000
VERSION     DAOSOU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11560_length_6668_cov_5.899743"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLW15_06930"
     CDS             <1..946
                     /locus_tag="PLW15_06930"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOT34162.1"
                     /translation="TLNAVCLSGTEAVAWAARLIDSGEVDIAVAVGQESMSLAPHVWS
                     ARAGQRYGSIELLDTVEHDGLRDAFERRSMGESTEDGNGELALGRDAQDAFAAASHAR
                     AAASADYLAEEIAPYTIAGKRGDTVVSVDDGVRADTTVEGLAGLRPAFTISTGSITAG
                     TITAGNSSQITDGAAALVLMSPAAAAARGITGLATVEAHALVAGPDVRLHAQPANAIL
                     AALAKTDDTVADLAVVEINEAFASVGVHSTELLGVDAAIVNRNGGAIALGHPIGASGA
                     RIVGHLARQLHDLGPGTLGAVGICGGGGQGSAIVLRGL"
     gene            complement(1010..1570)
                     /locus_tag="PLW15_06935"
     CDS             complement(1010..1570)
                     /locus_tag="PLW15_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOT34163.1"
                     /translation="MAVRGCTAREHDGSRCRAAVAEGAPISLCTQHLLVAHDWIVRDV
                     GVTDLLPSPCLACGSRTGIRYPTGWICAICEWRVGELPDGEAEPARVDVVYYLRWRDR
                     IKIGTSGNPRGRLAALPYEELLAFERGDRRLEQRRHAQFSGQRIPGSEWFRVNDELLA
                     HVAELRVDEPWDQYHRWVSRAVALRG"
     gene            1638..1919
                     /locus_tag="PLW15_06940"
     CDS             1638..1919
                     /locus_tag="PLW15_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34164.1"
                     /translation="MTNETATQSAETVDATPASTPKPPSIHTRAIITWLAIFPLVSIG
                     MLVLGPITDHWFPVLRALVLTALVVPLAVYVVVPQLMRTHGTISRRRRR"
     gene            complement(1897..2556)
                     /locus_tag="PLW15_06945"
                     /pseudo
     CDS             complement(1897..2556)
                     /locus_tag="PLW15_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
     gene            complement(2612..3583)
                     /locus_tag="PLW15_06950"
     CDS             complement(2612..3583)
                     /locus_tag="PLW15_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOT34165.1"
                     /translation="MRAAVYRRFGGPEVVRVEDVAIPTPGPDEVLIRVHASTVSAADH
                     RTRSGEIPKGLRLLAGFSIGFLRPRRPVLGMDAAGIVEAVGSRVTRFRPGDEVIAMLG
                     ARFGGHAEYALISQNGGIAAKPANLDFADAVSVVFGGITALAFLNRVCTGPGNRVLVN
                     GASGAVGSAAVQLAKHRGAHVTAVTSAAHRDLVLGLGADEVIDYAVSDFAANGELYDV
                     IVDCVGNAPFERAAASVRPGGALLLVVTDRKGLFGAARQSRASGKVVSAASVPYTPES
                     MAALSRLAGSGVIRPVIDRNYPLADIVDAHRYVDAGHKAGSVILTLA"
     gene            complement(3580..4251)
                     /locus_tag="PLW15_06955"
     CDS             complement(3580..4251)
                     /locus_tag="PLW15_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HOT34166.1"
                     /translation="MTTTIARPTRQASRAGWLVPVGLILLTLIPIVAGAARVTELTGA
                     PAITADNARFVTSPIPVIVHIVGATLFSILGAFQFVPGLRRRNRWHRLAGRVLVPAGA
                     AVALSGIWMAVISEHAPADGVALSALRVIFASLMLAFIALGVRSIIRRDFVSHGAWMT
                     RAYALAVAAGTQAIVLIPQTIIFGKYEETSRAVFMGAAWVINLAIAELVIRRRAARRT
                     VRSAS"
     gene            complement(4248..4514)
                     /locus_tag="PLW15_06960"
     CDS             complement(4248..4514)
                     /locus_tag="PLW15_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34167.1"
                     /translation="MTAPRTTGPADNDRYEIRIRGQLDAQWSDWFEGFALTVSDDQTI
                     LTGRISDQAALHGLLRRIGNLGLTLVSVTQLTATPLNQNEGEEP"
     gene            complement(4519..5163)
                     /locus_tag="PLW15_06965"
     CDS             complement(4519..5163)
                     /locus_tag="PLW15_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34168.1"
                     /translation="MRITPTALTKVAALAAMLAGLIYIVIQFIHPADVVESLTTPMWT
                     IVHVLSFAEAVLVMIGLAGIYLRQVREFGVLGLIAYAMFGFFFILQAAFNFAEAFIAP
                     LIAVDAPQLAVDIVGLFGRYPAVNDLGPLAALPQVGAVLYVGGALLFGVSIIRARVLS
                     RGAGLLLIVAAVITPVAGALLPHALERMAAIPMGLGIIWLGLSLWMDQRKTTAN"
     gene            complement(5275..>6668)
                     /locus_tag="PLW15_06970"
     CDS             complement(5275..>6668)
                     /locus_tag="PLW15_06970"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT34169.1"
                     /translation="RAARLIEAAIPGVRKSRQDAQLLRWLAQLPEETVGRMPVLSVFS
                     AWAHILRGELDGVEPWLVTAERLLASGDHDSGDGEELRSLPVTMSLYRAAVAQASGDL
                     AGVQTNAQRASDLAAPDDHLGRGAAAGMLGLAAAARGDLEAGVRAFGESATNLRLAGN
                     LTDALSTATVVADMLLPLGRLPEAIRAYEDALRIATDEGLADGRGEPPVADLHSGLAE
                     LLIEAHELESARDHLATAEALGDHAFSPEHRFRWFVSMARLREAEGDPDAALDLLASA
                     QRHYRRGFFPDARPIGAMRSRILIEQGRLEEAAAAIGELGLTGDDEPAYLSEYGQITL
                     ARLLLSQGRTGEATELLGRLLAAAEAGGRAARVTEIRELLGGASSAGRVGTAERVGTA
                     GRVGSATPELLSERELDVLRLLATELSGPEIARQLYVSLNTMRTHTKHIFDKLEVNSR
                     PAAVRRGRELNLL"
BASE COUNT         1041 a         2216 c         2461 g          950 t
ORIGIN      
        1 gaccctcaac gccgtgtgcc tttccggcac cgaggctgtc gcttgggcgg cccgcctgat
       61 cgactccggc gaggtcgaca tcgccgtcgc ggtcggccag gagtcgatgt cgctggcccc
      121 gcacgtgtgg agcgcccgcg ccggccagcg ctacgggtcg atcgagctgc tggacacggt
      181 cgagcacgat ggccttcggg acgcgttcga gcggcggtcc atgggggagt cgacggagga
      241 cgggaacggg gagttggctc tggggcggga cgcgcaggat gcgttcgcgg cggcgtcaca
      301 tgctagagcc gcggcatccg ccgactatct ggccgaggag atcgcgccgt acacaatcgc
      361 cggcaagcgc ggcgacaccg tcgtgagcgt tgatgacggc gtgcgcgccg acacgaccgt
      421 ggagggactg gccgggttgc gtccagcgtt cacgatctcg acgggctcga tcaccgcggg
      481 caccatcacc gccggcaact cgtcgcagat caccgacggg gcggcggcac tcgtgctcat
      541 gagtcccgcg gcagcggccg cgcgcgggat caccggcctc gcgaccgtgg aggcccacgc
      601 gctcgtcgcg ggccccgacg tgcgcctgca cgcccagccg gccaacgcga tcctcgcggc
      661 gctcgccaag accgacgaca ccgtcgccga cctcgcggtc gtggagatca acgaggcctt
      721 cgcgtctgtc ggcgtgcact cgaccgagct gctgggggtg gatgccgcga tcgtcaaccg
      781 caacggcggc gcgatcgccc tcggccaccc gatcggcgca tccggtgcac gtatcgtcgg
      841 gcaccttgcc cggcaactgc acgacctcgg ccccggaacg ctcggcgcgg tgggcatctg
      901 cggcggtggc ggccagggct cggcgatcgt gttgcgcggc ctctaggcct aggcaggttg
      961 agtagcggcg aagccgcgta tcgaaaccgg tgatcgagcc ggtcgagatc tacccccgca
     1021 gcgccaccgc gcggctcacc cagcggtggt actggtccca cggctcatcc acccgcagct
     1081 cggcgacgtg ggcgagcagc tcgtcgttca cccggaacca ctcgctgccc gggatgcgct
     1141 gccccgagaa ctgcgcgtgg cgtcgttgct cgagccgccg gtcgccgcgc tcgaaggcca
     1201 gcagctcctc gtagggcagg gccgcgagcc gaccgcgggg gttgccgctc gtgccgatct
     1261 tgatgcggtc gcgccagcgc aggtagtaga cgacgtcgac gcgggcaggc tcggcctcgc
     1321 cgtcgggcag ctcgccgacc cgccactcgc agatggcgca gatccagccg gtggggtagc
     1381 ggatgcccgt gcgcgacccg cacgccaggc acggcgacgg cagcaggtcg gtcaccccga
     1441 cgtcgcgcac gatccagtcg tgggcgacga gcaggtgttg ggtgcacagc gagatcgggg
     1501 cgccctcggc gacggccgcg cggcaccggc tgccgtcgtg ttcgcgagcc gtgcatccgc
     1561 gaactgccat gccagccacg gtacgcgccg ccgccgacgc gggactattc tgcgaggtcg
     1621 gagcgttgtg gtttctcgtg accaacgaga ccgcgaccca gtctgccgag accgtggatg
     1681 ccacccccgc atccaccccg aagcctccgt cgatccacac ccgcgcgatc atcacgtggc
     1741 tcgcgatctt cccgctggtc tcgatcggca tgctggtgct cggcccgatc acggatcact
     1801 ggttcccggt gctgcgggcg ctggtactga cggcgctcgt tgttccgctc gcggtgtacg
     1861 tggtggtgcc tcagctcatg cgcacgcacg gcaccatcag tcgtcggcgt cggcgctgac
     1921 cggctcgccc gaccccgcat caagccggaa cggcgggtac tcgtcgcgca tgagcgcgac
     1981 gtagacgatc acccgcagca tccaccggtt cagtcccatg atgaagtcga agaggctgcg
     2041 cgggtaggtg ccggtgatca gcacgaacac tcccgcgatc acgacgagca ggttgaggat
     2101 cgagtacccg gaggcgatgc tgcccggagt cgccgcgctc cacgggtaca ccagcaggtc
     2161 ggcgacgatg agcccgacga tgaccaggtg cggcacggcg agcagccact tcaccagcac
     2221 gagtccgcgc gagagccgct cggggtactc gacctcgatc tcggcggggt agtcggcgcg
     2281 accgagcgtg aacggcgggt accggtcggt gccgagcgcg gcatacacgt agaagcccac
     2341 ccgccagtgc cagcgcagca cgcccgtggt gaagttccac agcggtcgcg ggtaccgacc
     2401 ggtgaacagg atggtgattc cggctatgag ggttacaact atcgccgcgg cccacagcac
     2461 ggccagagcg acgaagtgcg ggatcgcgag gaacatcttc accagccaga gccagcggga
     2521 ggggtgctcg tcgagctcgc cggtgacgcg cacgggcgag tgggggaggg tggtcatggt
     2581 ggttcctgtc tgttgggtgg gtgggaagtg ttcaggcgag cgtcaggatg acgctgcccg
     2641 ccttgtggcc ggcgtcgacg tagcggtgcg cgtcgacgat gtcggccagg ggatagttgc
     2701 ggtcgatcac cgggcggatc acacccgaac ccgcaaggcg cgagagtgcg gccatcgatt
     2761 cgggggtgta gggcaccgaa gcggccgaga cgaccttgcc gctcgcgcga ctctggcgcg
     2821 cagcaccgaa gagtcccttg cggtcggtga cgaccagcag cagggctcca cccggtcgaa
     2881 cggatgccgc cgcccgctcg aacggcgcat taccgacgca gtccacaatc acgtcgtaga
     2941 gctcaccgtt cgcggcgaag tcggacaccg cgtagtcgat gacctcgtcg gcgccgaggc
     3001 cgagcacgag gtcgcggtgg gcggcgctgg tgacggccgt gacgtgggcg ccgcggtgtt
     3061 tggcgagctg cacggcggcg ctgccgacgg cgccggaggc tccgttgacc agcacccggt
     3121 tgcccggacc cgtgcacact cggttgagga acgcgagggc ggtgatgccg ccgaagacca
     3181 cgctgaccgc gtcggcgaaa tcgaggttcg cgggcttcgc agcgatgcca ccgttctggg
     3241 agatcagcgc gtactcggca tgaccgccga agcgggctcc aagcatcgcg atgacctcgt
     3301 ctccggggcg gaagcgggtg acacgcgagc cgacggcctc gacgatgccc gcggcatcca
     3361 tgccgagcac gggtcgccgg ggtcggagga agccgatgga gaagccggcg agcaggcgaa
     3421 gtcccttcgg gatctcgccg ctgcgggtgc ggtggtcggc agcgctgacg gtgctcgcgt
     3481 gcacccggat gagcacctcg tcggggccgg gggtcgggat ggcgacatcc tcgacccgca
     3541 cgacctcggg gccgccgaag cgacggtaga cggcggccct catgatgcgc tccgcactgt
     3601 gcgacgagcg gcgcggcgac ggatgacgag ctcggcgatg gccaggttga tgacccacgc
     3661 ggcgcccatg aacacggccc gcgaagtctc ctcgtacttg ccgaagatga tcgtctgcgg
     3721 gatgagcacg atggcctggg tgccggcagc gacggcgagg gcgtaggccc gcgtcatcca
     3781 ggcaccgtgg ctgacgaagt cgcgacggat gatcgatcgc accccgagcg cgatgaatgc
     3841 cagcatcagg ctcgcgaaga tcacacggag tgcgctgagc gcaacgccgt cggccggggc
     3901 gtgctcggag atcacggcca tccagatgcc ggacagggcg acggccgccc cggcgggaac
     3961 gagcactcgg cccgcgaggc ggtgccagcg gttgcggcga cgcagccccg gcacgaactg
     4021 gaacgcgccc aggatgctga acagggtcgc gcccacgatg tgcacgatga cggggatcgg
     4081 tgaggtgacg aagcgggcgt tgtctgccgt gattgcggga gcgcccgtca gctcggtcac
     4141 gcgggcggcg ccggcgacga tggggatgag cgtcagcagg atgaggccga cgggcacgag
     4201 ccagccggct cgcgatgcct gccgggtcgg gcgggcgatg gtcgtggtca tggttcttct
     4261 ccttcgtttt ggttgagtgg tgtagcggtg agttgtgtga cggagaccaa ggtcaggccc
     4321 agatttccga tgcgtctgag tagtccgtgg agggcagcct gatcgctgat gcggccggtg
     4381 aggatggtct ggtcgtcgct gacggtcagc gcgaatccct cgaaccagtc gctccattgg
     4441 gcgtcaagct gccccctgat gcggatctcg taccggtcgt tgtcggccgg tccggtggtg
     4501 cgtggtgcgg tcatcggact agttggcggt ggtcttccgc tggtccatcc agagcgacag
     4561 gccgagccag atgatgccga gacccatggg gatcgcggcc atgcgctcga gcgcgtgggg
     4621 caggagggcg ccggcgacgg gcgtgatcac ggcagcgacg atcagcagga ggcccgcgcc
     4681 gcgcgagagc acgcgagccc ggatgatcga cacaccgaac agcagcgcgc caccgacata
     4741 gagcacggca ccgacctgcg gcagcgcggc gagcgggccg aggtcgttga ctgcggggta
     4801 gcggccgaag aggccgacga tgtcgacggc gagctgcggg gcgtcgacgg cgatcagcgg
     4861 tgcgatgaag gcctccgcga agttgaacgc ggcctggagg atgaagaaga acccgaacat
     4921 cgcgtaggcg atgaggccga gcacgccgaa ttcgcgcacc tggcgcaggt agatcccggc
     4981 gaggccgatc atcacgagca ccgcctcggc gaagctcagc acgtgcacga tcgtccacat
     5041 cggggtcgtg agcgactcga cgacatcggc ggggtggatg aactggatga cgatgtagat
     5101 caggcccgcg agcatggccg cgagtgcggc gaccttggtg agggcggtgg gggtgatgcg
     5161 catgactgga ctccttcgac aggtccagac ggggtgccgg gacgtgccga atctagggag
     5221 cgacgtggtg accgggcatc accacatgtg gtgacccggg tggtgatcgt gcttctagag
     5281 caggttgagc tcgcgaccgc gacgcaccgc ggccggtcgg ctgttgacct cgagcttgtc
     5341 gaagatgtgc ttggtgtggg tgcgcatggt gttgagcgag acgtacagct ggcgcgcgat
     5401 ctcggggcca gacagctccg ttgccagcag cctcagtacg tcgagctcgc gttcgctgag
     5461 cagctccggt gtcgcacttc cgacccggcc cgcggttccg acccgctctg cggtgccgac
     5521 ccggcccgcg gatgacgcgc cccccagcaa ctcgcggatc tcagtaaccc gtgccgcacg
     5581 ccccccggcc tccgccgcgg cgagcagccg cccgagcagc tcggtcgcct cgccggtgcg
     5641 accctgcgac agcaggagcc tcgccagcgt gatctggccg tactcgctga ggtaggccgg
     5701 ctcgtcgtcg cccgtcagcc ccagctcgcc gatcgcggcg gccgcctcct cgaggcggcc
     5761 ctgctcgatc aggatgcgtg agcgcatcgc accgatcggt cgggcatcgg ggaagaaccc
     5821 gcgccggtag tgacgctgcg ccgacgcgag caggtcgagc gcggcatccg ggtcgccttc
     5881 ggcttcgcgc agccgggcca tggagacgaa ccagcggaac cggtgctcgg gggagaaggc
     5941 gtggtcgccc agagcctctg ccgtggccag gtgatcgcgg gcggactcca gctcgtgagc
     6001 ttcgatgagc agctcggcga gacccgagtg caggtcggca accggaggct cgccgcggcc
     6061 gtcggcgagc ccctcgtcgg tggcgattcg cagcgcgtcc tcgtaggcac ggatagcctc
     6121 cggcagacgc cccagaggta ggagcatgtc ggcgacgacc gtggcggtgc tcagcgcgtc
     6181 ggtgaggttg cccgccagtc gcaggttcgt ggccgactcc ccgaaggccc gcacccccgc
     6241 ctcgaggtcg ccgcgcgccg cggcggcgag ccccagcatg ccggcggccg caccccgccc
     6301 gaggtggtcg tcgggtgcgg ccagatcgct ggcgcgctgg gcgttggtct gcacgcccgc
     6361 gaggtcacca ctggcctgcg cgacagctgc ccggtagagc gacatggtca ccggcagcga
     6421 tcgaagctcc tcaccgtcgc cagagtcgtg gtcgccgctg gcgagcagcc gctctgcggt
     6481 gacgagccat ggttcgacgc cgtcgagctc gccgcgaaga atgtgcgccc acgcggagaa
     6541 gacgctcagc acgggcatgc gcccgacggt ctcctcgggc agctgggcga gccagcgcag
     6601 cagctgggca tcctgccggc tcttgcgcac cccgggaatc gccgcctcga tcagacgggc
     6661 cgcccgcg
//
LOCUS       DAOSOU010000106         6639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_11633_length_6639_cov_7.403554, whole genome shotgun sequence.
ACCESSION   DAOSOU010000106 DAOSOU010000000
VERSION     DAOSOU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11633_length_6639_cov_7.403554"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLW15_06975"
     CDS             complement(<1..644)
                     /locus_tag="PLW15_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOT34170.1"
                     /translation="MSKRIEVNDLNVYYGKFKAVEDVTLRIEPRTVTAFIGPSGCGKS
                     TFLRTLNRMHEVIPGAWVDGEVLIDGNNLYGPGVDPVLVRRQVGMVFQRPNPFPTMSI
                     RDNVLAGVKLNNTRMSKSDGDALVEKSLQGANLWNEVKDRLHLPGSGLSGGQQQRLCI
                     ARAIAVSPEVILMDEPCSALDPISTLAIEDLIEELKTEYTIVIVTHNMQQASRVS"
     gene            complement(669..1751)
                     /gene="pstA"
                     /locus_tag="PLW15_06980"
     CDS             complement(669..1751)
                     /gene="pstA"
                     /locus_tag="PLW15_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT34171.1"
                     /translation="MTTTTASTGIVNSLTTGRLPKWAPWALLAGSWLAMCAVFALVAA
                     ANGSEFNIVLSIFLGTVLFDVLIFVVAYLVEGVRQAKNRLVTSLVLTAFIIALLPLIS
                     LLFTVVVSGAARFDPLFFTSSMRNVVGEGGGALHAIMGTLLITGMATLISVPVGLLTA
                     IYLVEYGRGPLARAITFFVDVMTGIPSIVAGLFAFAFFALVFSDPGIRFGFGGSVALS
                     LLMIPVVVRSSEEMLKIVPNELREASYALGVPKWLTILKIVIPTSIAGITTGVTLAIA
                     RVIGETAPLLIISGFTASMNYDLFSDRMMSLPVFVYTQYANPGSVVQPYLDRAWAGAL
                     TLILIVMALNLIARLIARIFSPKLGR"
     gene            complement(1751..2689)
                     /gene="pstC"
                     /locus_tag="PLW15_06985"
     CDS             complement(1751..2689)
                     /gene="pstC"
                     /locus_tag="PLW15_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT34172.1"
                     /translation="MTTGTPSLRAKARPADRIFAGSTIVAGSLILAALAAVAVFLVAQ
                     SIPALVADPAELKGSPSSFWAYVGPLAFGTVWAALLALIMALPLSLGIALFISHYAPR
                     RVAQGLGYVIDLLAAVPSVVFGLWGIKVLAPAVTPLYGWLVDNLGWIPLFAPPVSGTG
                     RTILTVAIVLAVMIIPIITAITREVFLQTPVLHEEAALALGATRWEMIQMAVLPFGRP
                     GIISAAMLGLGRALGETMVVAMVLSPAALITIVLTGSQNPSTIAGNIALNFPEAHGVG
                     VNALIATGLILFVITLVINSIARFVINRRKAFSGAN"
     gene            complement(2827..3918)
                     /locus_tag="PLW15_06990"
     CDS             complement(2827..3918)
                     /locus_tag="PLW15_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOT34173.1"
                     /translation="MNFKRLGTIGAIAVAGTILLSSCAANEGGTTEPTTGPALSGTID
                     GAGASSQGSAQEAWIAAFQTANPDLTINYDPSGSGAGREAFIAGGVAFAGTDSSLKQE
                     EIDGGFAGCTPDTGYIQVPAYISPIAVIFNVEGVDKLNLDAATLAKIFKGDITTWDDA
                     AIVALNKDAKLPSAPITAVHRSDDSGTTKNFADYLFQNAPDVWTEKPADPFPYATGEA
                     AQGTSGVVDAVTNGTNTIGYADASKAGDLGTAAIQVGTEFVNYSAEAAAKVVDESPAG
                     DTNNGTTDLSIKINRTTTDATHYPLVLVSYIVTCTEFVDAEVAPLVKGYITYITSQDG
                     QAEAAASAGSAPLSAALSDKVATALEAIK"
     gene            complement(4073..>4684)
                     /locus_tag="PLW15_06995"
     CDS             complement(4073..>4684)
                     /locus_tag="PLW15_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT34174.1"
                     /translation="SLEWLPIDEVHDRLSYPHDREVIDRFAELLMTGRARTFAIIAVR
                     HGKAVPPGTWDGPDSSRPLMQRGADQATQIAAAIAAYGPTKIISSTAVRCRSTIEPLS
                     QLTALPVKTTAGISQDAFEHGQATVEKIVGKRLRKQKTVVLCSHGPVLPQIVGEVAAQ
                     TKSAVTADMRRSVSLGTGDFTVLHVSCEHPLSGLVAVETHSPD"
     assembly_gap    4685..4784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4789..>6639)
                     /locus_tag="PLW15_07000"
     CDS             complement(4789..>6639)
                     /locus_tag="PLW15_07000"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOT34175.1"
                     /translation="AEVSAKAHELQERHARAFRESLKPQLDDAGIHVETWADLGEADR
                     LSLDEIFSSQIFPVLMPLAVDPAHPFPYISGLSLNLSIRVRNPKSGKEEFARLKVPTN
                     LPRFIQLPDDASGRLRYIPLEDLISNHLGDLFPGMEIIAHHEFRVTRNEDVEVEEDES
                     ENLIQALEKQILNRRFGPPIRLEITDDMDAVTLGLLVRELDITEQEVYRLPAPLDLGG
                     LFQLNRIDRPELKYPRHVPTTSVNLLPVEPNAAPDIFASIARQDVLVHHPYESFSTSV
                     QAFLEQAALDPNVLAIKQTLYRTSGDSPIVEALIAAAEAGKAVLALVEIKARFDETAN
                     ISWARKLEKAGVHVVYGLVGLKTHCKLALVVRQEGGVLRHYSHIGTGNYNPKTSRIYE
                     DLGLFTADDQVGKDLTRLFNELSGYAIEKKFKRLLVAPLHLRNGLLKRINAEAANARE
                     GLPSGIRIKLNSIVDEAIIDALYRASNAGVPVELVVRGICGLKPGQPGLSENIKVRSI
                     LGRYLEHARIFSFHNNGDPQVFIGSADMMHRNLDRRVEALVRLTSPDHLRELDDLFDL
                     AMSDDTSSWWLEPDGTWTRHNRAQDGAPLQDMQNVLMKQISQRKRGGVLR"
BASE COUNT         1121 a         1941 c         2368 g         1109 t
ORIGIN      
        1 gagacacggg atgcctgctg catgttgtgc gtgacgatca cgatcgtgta ctcggtcttg
       61 agctcctcga tcaggtcctc gatagcgagg gtggagatcg ggtcgagtgc cgagcacggc
      121 tcgtccatga ggatgacctc gggcgagacc gcgatcgcgc gagcgatgca gaggcgctgc
      181 tgctggccac cggagaggcc cgagccgggc aggtgcaggc ggtccttgac ctcgttccag
      241 aggttggcgc cctgcaggct cttctcgacg agagcgtcgc catccgactt cgacatgcgg
      301 gtgttgttga gcttgacccc ggcgagcacg ttgtcgcgga tcgacatcgt ggggaacggg
      361 ttcgggcgct ggaacaccat gcccacctgg cgacgcacga gcaccgggtc gacgccgggg
      421 ccgtagaggt tgttgccgtc gatcagcacc tcaccgtcca cccaggcgcc ggggatgacc
      481 tcgtgcatgc ggttgagcgt gcgcaggaac gtcgacttgc cacagccgga ggggccgatg
      541 aaggccgtca cggtgcgggg ttcgattctg agcgtgacat cctcgacggc cttgaacttg
      601 ccgtagtaga cgttcaggtc gttgacctcg atgcgcttgg acacgggttt ccttatctgt
      661 gctgaggcct agcggccgag cttgggggag aagatgcggg caatgagtcg cgcgatgagg
      721 ttgagcgcca tcacgatcag gatcagggtg agtgcgcccg cccaagcgcg gtcgaggtag
      781 ggctgcacga ccgagccggg gttcgcgtac tgcgtgtaca cgaacaccgg aaggctcatc
      841 atgcggtcgc tgaacaggtc gtagttcatg ctcgcggtga atccggagat gatgagcagg
      901 ggcgcggtct cgccgatgac gcgggcgatg gccagcgtga caccggtcgt gatgccggcg
      961 atcgacgtgg ggatgacgat cttgaggatc gtcagccact tcggcacacc cagcgcgtac
     1021 gaggcctcgc gcagctcgtt gggcacgatc ttgagcatct cctcgctcga gcgcaccacc
     1081 acggggatca tcagcagcga cagcgctacc gagccaccga agccgaaccg gatgcccggg
     1141 tctgagaaca ccagggcgaa gaaggcgaaa gcgaacagac cggccacgat cgacgggatg
     1201 cccgtcatga catccacgaa gaaggtgatc gcccgtgcga gcggcccgcg accgtactcg
     1261 accaggtaga tcgcggtgag cagaccgacg ggcacggaga tgagcgtcgc catgccggtg
     1321 atcagcagcg tgcccatgat cgcgtgcagc gcaccgccac cttcgccgac cacgttgcgc
     1381 atcgagctgg tgaagaacag cgggtcgaag cgggcggctc cgctgaccac gaccgtgaag
     1441 agcagcgaga tcagcggcag cagggcgatg atgaacgccg tgagcacgag cgaggtgacg
     1501 aggcggttct tggcctgacg aacgccctcg accaggtagg cgacgacgaa gatcagcacg
     1561 tcgaagagca cggtgccgag gaagatcgac agcacgatgt tgaactcgct gccgttcgcg
     1621 gcggcgacga gagcgaacac ggcgcacatg gcgagccaac tgccggccag gagcgcccac
     1681 ggcgcccact tgggaagtct gccggtggtc agggagttca cgatgcccgt gctggcggtc
     1741 gtcgtggtca tcagttggct cccgagaatg ccttgcggcg gttgatcacg aagcgcgcga
     1801 tcgagttgat cacgagcgtg atgacgaaca ggatgagtcc ggtcgcgatg agggcgttga
     1861 cgccgacacc gtgagcctcg gggaagttca gggcgatgtt gccggcgatg gtcgaggggt
     1921 tctgcgaacc cgtgagcacg atcgtgatga gggccgcagg cgagagcacc atggcgacca
     1981 ccatggtctc gccgagggcg cgacccagac cgagcatggc ggccgagatg atgccggggc
     2041 gaccgaacgg cagcaccgcc atctggatca tctcccagcg ggtggcgccg agcgcgagcg
     2101 ccgcctcctc gtggagcacg ggggtctgca ggaagacctc gcgggtgatg gcggtgatga
     2161 tcgggatgat catgacggcg agcacgatcg cgacggtgag gatggtgcga cccgttccgg
     2221 agaccggagg ggcgaacagc gggatccagc ccaggttgtc gaccagccag ccgtagagcg
     2281 gggtgacggc gggggccaga accttgatgc cccagaggcc gaagacgacc gagggcacgg
     2341 cggccagcag gtcgatgacg tagccgaggc cctgggcgac gcgccgcggg gcgtagtgcg
     2401 agatgaagag ggcgatgccg agcgagagcg gcagtgccat gatcagggcg agcagtgccg
     2461 cccagacggt tccgaaggcg agcggcccga cgtaggccca gaagctcgac ggtgagccct
     2521 tgagctcagc ggggtcggcg accagtgcgg ggatgctctg cgcgaccagg aagacggcga
     2581 ctgcggcgag ggccgcgagg atgaggctgc cagcgacgat ggtgctgccg gcgaagattc
     2641 ggtcggccgg gcgggctttc gctcgcagcg acggggttcc tgtcgtcatt cctgtcttcc
     2701 tgcgcgacct gggtggtgtg gatcttgggg ggaggattcg gggagggccg gtgctctaca
     2761 gtctgtcggg ctgcgaccct gcgtgcaatg cacgccagcc gcagcccgac agttactgca
     2821 caggtgctac ttgatcgcct cgagagcggt cgcaaccttg tccgacaggg cggccgagag
     2881 cggcgccgag ccggcggaag cggcagcctc agcctggcca tcctggctgg tgatgtaggt
     2941 gatgtaaccc ttgaccagcg gggcaacctc agcgtcaacg aactcggtgc aggtgacgat
     3001 gtagctcacc agaacgagcg ggtagtgggt cgcgtcggtc gtggtgcggt tgatcttgat
     3061 cgacaggtcc gtggtgccgt tgttcgtgtc accggcgggg gactcgtcga caaccttggc
     3121 cgcagcctct gccgagtagt tcacgaactc ggtgccgacc tggatggccg ccgttccgag
     3181 gtcgccagcc ttggaggcgt cagcgtaacc gatggtgttg gtgccgttgg tcactgcgtc
     3241 gacaacaccc gaggtgccct gagcggcctc accggtcgcg taggggaacg ggtcagcggg
     3301 cttctcggtc cacacgtcgg gagcgttctg gaacaggtag tcagcgaagt tcttcgtggt
     3361 acccgagtcg tccgagcggt gcacggcggt gatgggggcc gaaggaagct tggcgtcctt
     3421 gttcagagca acgatggctg cgtcatccca cgtggtgatg tcgcccttga agatcttggc
     3481 gagcgtcgcg gcgtcgaggt tgagcttgtc aacaccctcc acgttgaaga tcacggcgat
     3541 cggggagatg taggccggaa cctggatgta accggtgtcg ggggtgcaac ccgcgaagcc
     3601 accgtcgatc tcctcctgct tgagcgagga gtcggtgccc gcgaacgcga cgccgccggc
     3661 gatgaacgcc tcacggccag cgccggaacc cgaggggtcg tagttgatgg tcaggtcggg
     3721 gttggcggtc tggaacgctg cgatccaggc ctcctgcgcc gatccctgcg acgacgcgcc
     3781 agcgccatcg atcgtgccgg agagagccgg acccgtggtc ggctcggtgg ttccaccctc
     3841 gtttgctgca caagaggaga ggaggatggt gcctgcaact gcaatggcac cgatggtgcc
     3901 aaggcgcttg aagttcacgt ttaacccttc ggaagtgtga ttgttactgc gggccagtgc
     3961 gacccacctg acgaacctaa gcactggggt taaccacgct cggcccgcaa ggtaaacgga
     4021 aggtgaacga cgccgtaacg actggcgcac ctgcgtgtgt tcgccgcgtg tttcagtcgg
     4081 ggctgtgggt ttcgacggca accaggcccg agagcggatg ctcgcacgaa acatgcagca
     4141 ccgtgaagtc gcccgtgccg agcgaaacgg accggcgcat gtcggcggtg accgccgact
     4201 tcgtctgcgc cgccacctcg ccgacgatct gcggcagaac cggaccgtgg ctgcagagca
     4261 ccacggtctt ctgcttgcgc agtcgcttgc ccacgatctt ctcgaccgtg gcctggccgt
     4321 gttcgaacgc gtcctggctg atgccggccg tcgtcttgac cggcagcgct gtcagctgcg
     4381 acaggggttc gatggtcgag cggcatcgca cggcggtgct cgagatgatc ttcgtgggtc
     4441 cgtacgcggc gatggctgcg gcgatctgcg tggcctgatc tgccccgcgc tgcatgagcg
     4501 gtcgcgacga atcggggccg tcccacgttc ccggcggcac cgccttgccg tggcgcacgg
     4561 cgatgatggc aaaggtgcgg gcccggcccg tcatgagcag ttctgcgaag cggtcgatga
     4621 cttcgcggtc gtgcgggtag ctgagccgat cgtgaacctc gtcgatcggc agccactcga
     4681 gggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcactc accgcagcac
     4801 tccgccgcgc ttcctctggg agatctgctt catgagtaca ttctgcatgt cctgaagggg
     4861 agcgccgtcc tgcgcgcggt tgtggcgagt ccaggtgccg tcgggttcga gccaccagct
     4921 ggacgtgtca tccgacatcg ccagatcgaa gaggtcgtcg agctcacgca ggtggtcggg
     4981 gctggtgagg cgcaccagtg cctcgacgcg acggtcgagg ttgcggtgca tcatgtcggc
     5041 gctgccgatg aacacctgcg ggtcgccgtt gttgtggaac gagaagatgc gcgcgtgctc
     5101 caggtagcgc ccgagaatcg agcgcacctt gatgttctcg ctcagcccgg gctggccggg
     5161 cttcaggccg cagatgccgc gcacgacaag ctcaacggga acgcccgcgt tgctcgcgcg
     5221 atagagcgcg tcgatgatgg cctcgtcgac gatcgagttc agtttgatgc ggatgcccga
     5281 cggaagaccc tcacgcgcgt tggcggcctc cgcgttgatg cgcttgagca gaccgttgcg
     5341 caagtggagc ggggcgacga gcaaccgctt gaacttcttc tcgatcgcgt agccggagag
     5401 ctcattgaag agacgtgtca ggtctttgcc gacctggtcg tcggcggtga acaggccgag
     5461 gtcctcgtag atgcggctcg tcttcgggtt gtagttgccg gtgccgatgt ggctgtagtg
     5521 gcgcagcacg ccaccctcct ggcgcacgac gagcgcgagc ttgcagtggg tcttgaggcc
     5581 gaccagaccg tagacgacgt gaacgccggc cttctccagc ttgcgcgccc acgagatgtt
     5641 cgccgtctcg tcgaagcgcg ccttgatctc gacgagtgcg agcaccgcct tgccggcctc
     5701 ggccgcggcg atgagcgcct cgacgatggg gctgtcgccg gaggtgcggt acagcgtctg
     5761 cttgatggcc agcacgttgg ggtcgagggc ggcctgctcg aggaacgcct gcacgctcgt
     5821 ggagaacgac tcgtaggggt ggtgcacgag cacgtcctgg cgggcgatcg acgcgaagat
     5881 gtccggagcg gcgttcggct cgaccggcag caggttgacg ctcgtcgtcg gcacgtgccg
     5941 cgggtacttg agctcgggtc ggtcgatgcg gttcagctgg aacaggccgc ccaggtccag
     6001 gggtgcggga agccggtaca cctcctgctc cgtgatgtcg agctcacgca cgagcaggcc
     6061 gagggtgacg gcatccatgt catcggtgat ctcgagacgg atcggcggac cgaaccgacg
     6121 gttgaggatc tgcttctcga gagcctggat gaggttctcg ctctcgtcct cctcgacctc
     6181 gacgtcttcg ttgcgggtga cgcggaactc gtggtgggcg atgatctcca tgcccgggaa
     6241 caggtcgccc aggtggttgg agatgaggtc ttcgaggggg atgtagcgca ggcgacccga
     6301 ggcgtcatcc ggcaactgga tgaagcgggg caggttcgtg ggaaccttca gccgcgcgaa
     6361 ctcctccttg cccgacttcg ggttgcgcac ccggatcgac aggttgaggc tgagcccgga
     6421 gatatagggg aatggatgcg ccgggtcgac ggcgagcggc atgagcaccg gaaagatctg
     6481 gctggagaag atctcgtcga gcgacagccg gtcggcctcg ccgaggtcgg cccaggtctc
     6541 cacgtggatg cccgcgtcgt cgagctgcgg cttgaggctc tcgcggaacg ctcgcgcgtg
     6601 ccgctcctgc agctcgtgcg ccttcgcgct cacctcggc
//
LOCUS       DAOSOU010000107         6473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12031_length_6473_cov_6.718604, whole genome shotgun sequence.
ACCESSION   DAOSOU010000107 DAOSOU010000000
VERSION     DAOSOU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12031_length_6473_cov_6.718604"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PLW15_07005"
     CDS             complement(<1..1232)
                     /locus_tag="PLW15_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT34176.1"
                     /translation="MAAARTASTKKSPKNGRSASRLSFAKITDTLTVPDLLALQTESF
                     DWLVGNDIWKQRVAEGVAAGRTDLPSRSGLDEIFEEISPIEDLGETMQLSFTDPELEE
                     PKYTIDECKERGKTFAAPLYVNAEFMNHLTGEIKTQTVFMGDFPLMTEKGTFIINGTE
                     RVVVSQLVRSPGVYFDRTPEKLSDKDIYSARIIPSRGAWLEFEIDKRDQVGVRIDRKR
                     KQSVTVFLKALGLTSEQILEEFKGFPSIELTLEKDSILTKEEALKDIYRKLRPGEQVA
                     AEAARALLDNFYFNTKRYDLAKVGRYKINRKLGLDTDIKQSVLTIEDIIATIKYLVAL
                     HDTSSPYVTVHDNSLATIKGIRDGKKVDVRLDVDDIDHFGNRRIRAVGELIQNQVRTG
                     LSRMERVVRERMTTQDIEA"
     gene            complement(1474..2475)
                     /gene="speB"
                     /locus_tag="PLW15_07010"
     CDS             complement(1474..2475)
                     /gene="speB"
                     /locus_tag="PLW15_07010"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOT34177.1"
                     /translation="MTQPRRHVPPGLRAQLDAPSYQGPSTFGMRPLLTEPEQLDEWQP
                     DVAVVGAPWDDSTTNRPGARFGPRALRASAYDPGTYHLDLGIEIFDHLEVVDYGDAIT
                     SHGMWELSRAAIHERVAEVATRGIVPFIIGGDHSITWPAATAVAEHHGFGTVTMIHFD
                     AHADTANILEGNLASHGTPMRRLIESGAIPGDRFIQVGLRGYWPGEEDQKWMRDNGLR
                     HFMMQEFWERGIPAVLEDLVLAAKERGDKTYISIDIDVLDPGFAPATGTPEPGGFAPI
                     DLLRIIRRLVLETDVVAFDVMEVAPAYDHADLTVNVAHRLIWEAFAALAVKKRAAAT"
     gene            complement(2493..2876)
                     /locus_tag="PLW15_07015"
     CDS             complement(2493..2876)
                     /locus_tag="PLW15_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HOT34178.1"
                     /translation="MIANRKDSSVSLYEQVGGEAAIGALVDSFYHRMLADPEVNEWFR
                     GIDLNRLKEHQRAFLAVGLGGPEAYDGRSMRTAHTGLAITQDAYTTAVAHLTEALIQL
                     GVDEPIVRQVIKRIETMRAAIVEVR"
     gene            2934..3818
                     /locus_tag="PLW15_07020"
     CDS             2934..3818
                     /locus_tag="PLW15_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOT34179.1"
                     /translation="MWQGRGVPDYPSRTLSVSGLYAEITPDRWVPGSFTLVVDGTPQS
                     HVNLDDPSQLFFEYIQRMGHVIDQLRMPGEPITAVHLGAGALTLPRYVEATRPGSRQQ
                     VIELEPDLVDLVRTELPWSTRASIRVRHGDAREVLEKLPRGLHGTVDLLVVDVFGGAR
                     TPAHVTSLEFYTSAVALLAPGGILLANVADGPGLAFARGQAATLQAAIGHVAALAETQ
                     ILKGRRFGNIVLVGSASDLPFDWMPRLLAAGPHPSKVVAGPELREFIAGAPVVTDATA
                     IPSPPPSKNIFQVRPGQS"
     gene            3830..4939
                     /locus_tag="PLW15_07025"
     CDS             3830..4939
                     /locus_tag="PLW15_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOT34180.1"
                     /translation="MRFLPAAAALMLLAGCSATPVAVSPSPTTVAPPAVSPTAAAAPP
                     VAPGGSPTVIASGLQTPWSIVRLRSGSTLISERDPGVIVELTATGTVREVGAVADAVH
                     EGEGGLLGLAYVRARGWLFAYVTTATDNRIVRYALEGDPGSYSLGESTVILSGLAKAG
                     NHNGGRIKIGPDGLLYATVGDAGNPDLAQDLTSNNGKILRMELDGSVPTDNPIADSLI
                     YSLGHRNPQGLAWDADGQLWAAEFGQDTWDEFNRIVPGGNYGWPIVEGTAGDPAFMDP
                     AYQWATDDASPSGLAFVNGTFLLAALRGQRLWAVYPGETTTASEYYTGTYGRLRDVVP
                     GPDGTAWVLTSNTDGRGSPVSGDDRLLQIDLVPLG"
     gene            4949..5395
                     /locus_tag="PLW15_07030"
     CDS             4949..5395
                     /locus_tag="PLW15_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HOT34181.1"
                     /translation="MAELDRVRTWAHALIDLHLDPSWSFAFDNARTRAGLCNYTAKRI
                     SVSRHMALRSDDDEVHQVLLHEVAHALTGKDVGHGPQWRAVAADLGYAGGRTFDGPIA
                     KDLAPWLGTCPAGHEIHRHRRPTRPMSCGRCSRRFDAAHLIRWVRQ"
     gene            complement(5420..5830)
                     /locus_tag="PLW15_07035"
     CDS             complement(5420..5830)
                     /locus_tag="PLW15_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT34182.1"
                     /translation="MSDTTGLTKDAGWELGVRTTVAAPIDVVWEFLLGAGLPVWLGEI
                     DALPEEKGAAYSTADGVSGTIRSRTDGLRVRLSWRPSDWPHDSTLQLTVKSAATGTTI
                     GFHHEHLADREERRMMLGHWKNVAAALDAHFAAG"
     gene            5852..6454
                     /locus_tag="PLW15_07040"
     CDS             5852..6454
                     /locus_tag="PLW15_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HOT34183.1"
                     /translation="MAASTAAPPTGQWFRSSDGTQWWFDSGSLSLDFAYTGAMSDNPA
                     WEQLHAPADLATWLEQRFPAADGIVSEGDLRDALQLRGAVARAAASASRGEAPAPEDV
                     DIINLFAATPDIAPALEGGTRQAGRSRVRTGQALSAMAREAIGLLAAADEGRYRECAA
                     DDCQLVFHDESRSNNRRWCSMLRCGNRTKVRNHRARKATA"
BASE COUNT         1057 a         2141 c         2153 g         1122 t
ORIGIN      
        1 gcctcgatgt cctgcgtggt catgcgctcg cgaacgacgc gctccatgcg ggacaggccg
       61 gtgcgaacct ggttctggat gagctcgccg accgcgcgga tgcgacggtt gccgaagtgg
      121 tcgatgtcgt ccacgtcgag acgcacgtcg accttcttgc catcgcggat gcccttgatg
      181 gtggcgaggc tgttgtcgtg caccgtcacg tagggcgacg aggtgtcgtg cagcgcgacg
      241 aggtacttga tcgtcgcgat gatgtcctcg atggtgagca ccgactgctt gatgtcggtg
      301 tcgagaccca gcttgcggtt gatcttgtag cgacccacct tcgcgaggtc gtagcgcttc
      361 gtgttgaagt agaagttgtc gaggagcgcg cgggcggcct cggcggcaac ctgctctccc
      421 ggacgcagct tgcggtagat gtccttgagg gcctcctcct tggtgaggat ggagtccttc
      481 tcgagggtga gctcgatcga cgggaagccc ttgaactcct cgaggatctg ctcgctcgtc
      541 aggcccaggg ccttgaggaa cacggtgacg gactgcttgc gcttgcggtc gatgcgcacg
      601 ccgacctggt cacgcttgtc gatctcgaac tcgagccagg caccgcggct cgggatgatg
      661 cgtgccgagt agatgtcctt gtcggacagc ttctccgggg tgcggtcgaa gtagacaccg
      721 ggcgaacgca cgagctggga cacgacgaca cgctcggtgc cgttgatgat gaacgtgccc
      781 ttctcggtca tgagcgggaa gtcgcccatg aagaccgtct gggtcttgat ctcaccggtg
      841 aggtggttca tgaactcggc gttcacgtag agcggagcgg cgaaggtctt gccgcgctcc
      901 ttgcactcgt cgatcgtgta cttgggctcc tcgagctcag gatcggtgaa cgagagctgc
      961 atggtctcgc ccagatcctc gatcggggag atctcctcga agatctcatc gagaccgctg
     1021 cgcgacggaa ggtcggtgcg ccctgcggcg acaccctccg cgacacgctg cttccagatg
     1081 tcgttgccga cgagccagtc gaagctctcg gtctgcagtg caagcagatc gggaaccgtc
     1141 agggtgtcgg tgatcttggc gaaagaaagc cgtgaagctg aacggccgtt cttgggggac
     1201 tttttggtgg acgcagtgcg cgcagcagcc aaggaaataa cctccgtgga ccccgtcggg
     1261 tccttatgcc ggtgaaccgg ctgtcggtaa tgaaatgctc accaggaccc aaggaagatc
     1321 tgccccgccg ctatactcgg ggcgcgccag ctgtccacaa tatgacggca tgggttgtct
     1381 aggagcgcaa agaacaatca taggactgtt tgcgctgcgt gcgcaacttg tttcttgacc
     1441 ttgctgcgag attgggctat aagtgcccgt gcatcatgtc gccgcggccc gcttcttgac
     1501 ggcgagggca gcgaatgctt cccagatcaa gcggtgggcc acgttcacgg tgaggtctgc
     1561 gtggtcatac gccggggcga cttccatcac atcgaacgcc accacatcgg tctcgagaac
     1621 cagacgacgg atgattcgca ggaggtcgat gggcgcgaag ccgcccggct ccggagtgcc
     1681 cgtcgctggc gcgaatccgg gatcgagcac atcgatgtcg atcgagatgt aggtcttgtc
     1741 gccccgctcc ttggcggcga gaacaagatc ttccagcacc gccgggatgc cgcgctccca
     1801 gaactcctgc atcatgaagt gccgcagccc gttgtcgcgc atccacttct ggtcttcctc
     1861 gcccggccag tagcctcgca gcccgacctg gataaaccgg tcccccggga tcgcaccgga
     1921 ttctatgagt cgccgcatcg gcgttccatg actggcgagg ttgccctcga ggatgtttgc
     1981 ggtgtctgcg tgggcgtcga agtggatcat tgtcacggtg ccgaagccat ggtgttccgc
     2041 gacggcggtg gcagccggcc aggtgatcga gtggtcgcct ccgataatga atggcacgat
     2101 gccgcgagtg gcgacttcgg ctacccgctc atgaatcgcc gcgcgcgaca gttcccacat
     2161 tccgtggctc gtgatggcat ccccgtaatc gaccacctcg aggtggtcga agatctcgat
     2221 gcccaggtct aggtgatagg taccggggtc atacgcgctg gcgcgcagcg cgcgcgggcc
     2281 aaaccgagcg ccgggtcgat tggtcgtcga gtcatcccac ggggctccca cgacggcgac
     2341 atcgggctgc cactcgtcaa gctgctcggg ctcggtgagc agcgggcgca tcccgaatgt
     2401 cgacgggccc tgatacgacg gggcatcgag ttgggcgcgc agcccgggag gaacgtgtcg
     2461 gcgcggctgg gtcatgctcg atagtagacc gctcagcgca cctcgactat ggccgcgcgc
     2521 atcgtctcga tgcgcttgat gacctgacgc acgatcggct catccacgcc cagctgaatc
     2581 agggcctcgg tgaggtgggc gacggccgtc gtgtaggcat cctgcgtgat ggcgagaccg
     2641 gtgtgcgcgg tgcgcatgct gcgaccgtcg taggcctccg gcccaccgag accgaccgcg
     2701 aggaacgcgc gctggtgctc tttgaggcgg ttcaggtcga tgccgcggaa ccactcgttg
     2761 acctcggggt cagcgagcat ccgatggtag aaggaatcga cgagggcgcc gatcgccgcc
     2821 tcaccaccta cctgctcgta caacgacacc gaggagtcct tccgattcgc gatcatggcg
     2881 agtgtactga tcggtctagt attgacaaaa tctccacttc ggggggtcga gagatgtggc
     2941 agggtcgagg tgtgcccgac tacccctctc gaacgctctc agtgagcgga ctgtacgcgg
     3001 agatcacccc tgaccgttgg gtgcccgggt cgttcaccct cgtcgtcgac ggcacgccac
     3061 agtcacacgt caacctcgac gaccccagcc agctgttctt cgagtacatc cagcgcatgg
     3121 gtcacgtcat cgaccagctc cggatgcccg gcgagccgat cacggccgtg cacctcggtg
     3181 cgggcgccct gaccctgccg cgctacgtcg aggccaccag acccggatcg cgccagcagg
     3241 tgatcgagct cgagcccgac ctcgtggatc tcgtgcgcac cgaacttccg tggtcgaccc
     3301 gggcgtcgat ccgggtgcgg cacggcgacg cccgagaggt gctcgagaaa ctgccccgcg
     3361 gcctgcacgg caccgtcgac ctgctcgtgg tcgacgtgtt cggcggcgcc cgcacccctg
     3421 cgcatgtgac gagcctcgag ttctacacga gcgccgtcgc gctgctcgcg cccggcggca
     3481 tcctgctggc caatgtggcc gacggcccag ggctcgcctt cgcacgcggc caggccgcga
     3541 cgctgcaggc cgccatcggg catgtcgccg ctctcgccga gacccagata ctcaagggtc
     3601 ggcgcttcgg caacatcgtg ctcgtcgggt cggcgagcga cctgcccttc gactggatgc
     3661 cgcggctgct ggccgccgga ccgcatccgt caaaggtcgt ggccggcccc gaactgcgcg
     3721 agttcatcgc gggcgccccc gtggtcaccg acgcaacggc gattcccagc ccaccgccat
     3781 ccaagaacat ctttcaggtg cgtcccggcc agtcgtgatg agatggagca tgcgcttcct
     3841 gcccgctgcc gccgcgctca tgcttctcgc ggggtgctca gcgacgccgg tcgcggtgtc
     3901 gcccagtccg accactgtcg cgccgcccgc cgtgagtcct accgcggccg ctgcaccgcc
     3961 tgtcgcgccc ggcggtagcc ccaccgtgat cgcatcggga ctccagacgc cctggtcgat
     4021 cgtgcgtctg cgcagcggtt cgacgctgat cagcgaacgc gatcccggcg tgatcgtcga
     4081 gctgaccgcc accggaaccg tgcgggaggt cggcgcggtt gccgacgccg tgcacgaggg
     4141 cgagggcggg ctgctgggac tggcctatgt tcgcgcgcgc ggatggttgt tcgcctacgt
     4201 cacgaccgcg accgacaacc ggatcgtgcg ctacgcgctg gagggagatc cgggcagcta
     4261 ctcgctgggg gagagcaccg tcatcctcag tggtctcgcc aaggcgggca atcacaacgg
     4321 cggccgtatc aagatcggac cggacggcct gctctacgcg acggtgggcg atgcgggcaa
     4381 ccccgacctc gctcaggacc tgaccagcaa caacggaaag atcctgcgca tggagctcga
     4441 cggatcggtt cccaccgaca atccgatcgc ggactccctc atctactcgc tcggccaccg
     4501 caacccgcag gggttggcgt gggacgccga cggccagctc tgggccgcgg aattcggaca
     4561 ggacacctgg gacgagttca accgcatcgt gcccggcggc aattacggct ggccgatcgt
     4621 cgaaggcacg gcgggtgacc ccgccttcat ggatccggcc tatcagtggg cgacggatga
     4681 cgcgagcccg agcggcctcg cattcgtcaa cggcaccttc ctgctcgccg ccctgcgcgg
     4741 gcagcggctc tgggctgtct accccggcga gacgacgacc gcgagcgagt actacaccgg
     4801 aacatacggc aggctgcgcg acgtggtccc cggaccggac ggcacggcgt gggtgctcac
     4861 cagcaacacg gacggtcggg gcagtccggt ttcgggagac gaccgcctgc tgcagatcga
     4921 tctcgtaccc ctcggttaac ctcaagaggt ggctgaactt gatcgggtgc gcacctgggc
     4981 gcacgccctc atcgatcttc atctcgaccc gagctggagc ttcgccttcg acaatgcgcg
     5041 cactcgagca gggctctgca actacaccgc gaagcgcatc agcgtgtcac ggcacatggc
     5101 gctgcgcagc gacgacgacg aggtgcacca ggtgctgctg cacgaggtgg cgcacgcgct
     5161 caccggcaag gatgtcggtc acggcccgca gtggcgggcg gttgccgccg acctgggata
     5221 cgccggtggt cgcaccttcg acgggcccat cgcgaaggac ctcgccccct ggctcggcac
     5281 ctgccccgcc ggccacgaga tccatcggca ccggcgtccg acccgaccga tgtcgtgcgg
     5341 caggtgttcg cgacgcttcg acgccgcgca cctgattcgg tgggtgcggc agtagatcgt
     5401 tatgcggatt ggccgctgat caaccagcgg cgaagtgcgc gtcgagtgcg gccgcgacgt
     5461 tcttccaatg accgagcatc atgcgtcgtt cttcgcggtc ggccaggtgc tcgtggtgga
     5521 atccgatggt cgttccggtc gctgcggact tgactgtcag ctgcagggtc gaatcgtgcg
     5581 gccagtcgct cggccgccag ctcaggcgca cccggagtcc gtcggttcga ctgcggatcg
     5641 tgccgctcac gccgtcggct gtggagtagg cagcgccctt ctcctccggt agcgcgtcga
     5701 tctcgccgag ccagacgggc aggccggcgc ccagcagaaa ctcccagacc acgtcgatcg
     5761 gtgccgccac cgtcgtgcgc acccccagtt cccagccggc gtccttggtc agtccagtgg
     5821 tatcgctcat gtaaccgact atattggtta cgtggcagca tcgacagcag caccgccgac
     5881 cggccagtgg ttccggtcct ccgacggcac ccagtggtgg ttcgactccg gctcgctcag
     5941 cctcgacttc gcctacacgg gtgccatgag tgacaacccg gcgtgggagc agctgcacgc
     6001 tccggcggat ctcgcgacgt ggctcgagca gcgatttccg gccgccgacg ggatcgtgag
     6061 cgagggcgac ctcagggacg cactgcaact gcgcggcgcg gtggctcgcg cggcggcatc
     6121 cgcgagtcgg ggcgaggcgc cggcacccga ggacgtcgac atcatcaacc tgttcgcagc
     6181 cacacccgac atcgcgcccg ccctcgaggg cggcacccgt caggctggac gttcgcgggt
     6241 gcgaaccggt caggccctgt cggcgatggc ccgcgaggcg atcggccttc tcgccgcggc
     6301 cgacgagggt cgctaccggg agtgcgccgc ggatgactgc cagctcgtgt tccacgacga
     6361 gtcccggtcg aacaaccgcc gctggtgctc gatgctgcgc tgcggcaacc gaacgaaggt
     6421 gcgcaaccac cgcgcgagga aggcgacggc atgaccatca cccacatcaa ccc
//
LOCUS       DAOSOU010000108         6467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12053_length_6467_cov_5.823924, whole genome shotgun sequence.
ACCESSION   DAOSOU010000108 DAOSOU010000000
VERSION     DAOSOU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12053_length_6467_cov_5.823924"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..199)
                     /locus_tag="PLW15_07045"
     CDS             complement(50..199)
                     /locus_tag="PLW15_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34184.1"
                     /translation="MATRSGRWRNKGDERMSLGEHLRELRKRLVISAIAILVAVVAGY
                     FLAPT"
     gene            complement(205..477)
                     /gene="tatA"
                     /locus_tag="PLW15_07050"
     CDS             complement(205..477)
                     /gene="tatA"
                     /locus_tag="PLW15_07050"
                     /inference="COORDINATES: protein motif:HMM:NF001854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HOT34185.1"
                     /translation="MFGNAFGWPHLIVILAVVLLLFGAPKLPGLARSIGQSMRIFRSE
                     VKTMKDDDAAPVEDEEPEAPKASTAKTTTARSTAPKAAPKATKPKP"
     gene            complement(507..875)
                     /locus_tag="PLW15_07055"
     CDS             complement(507..875)
                     /locus_tag="PLW15_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34186.1"
                     /translation="MIEPVEIIPWWAWLLIWTGLVLGLLVMLGLFAWWLFRKFLTLTD
                     DLADLAERSALLEVDDPVLVKPEIAVLAEVRDIRDREAARRAHRAELRRQRHERRMAR
                     ARRITGPAAAQREWPADWVS"
     gene            complement(872..1846)
                     /locus_tag="PLW15_07060"
     CDS             complement(872..1846)
                     /locus_tag="PLW15_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT34187.1"
                     /translation="MAERAQPQKAQDKLAFLLSLVPYLMDHDRVSVAEAAAHFGVPEE
                     QIRDAVRLISVSGIPGETASYQHGDLFDISWDDFEFNDEIVLTNLVAIDDSPRFSARE
                     AAALIAGLQYLSALPEHADRAAIASLQSKLSRGASAVPSQVAVEASETDDTLRLIREA
                     VEHGVQLKFDYLNSRGEHENRHVDPLRVESVDADWYLRGWCHLREAVRTFRLDRISDP
                     IVTDEPITHGATDVTLPDTLFEGSADDLLVTIDVAAGAEALLADYVPDGATSTIVDGR
                     VRTTVRVSHYHGLKRLIASMPGVATVVAPPEARAVVAEWAAAGADRYR"
     gene            complement(1839..2834)
                     /locus_tag="PLW15_07065"
     CDS             complement(1839..2834)
                     /locus_tag="PLW15_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT34188.1"
                     /translation="MPPASSAARIPVEERLFSLVLALLATDTGLTKNEILSTVQGYRQ
                     KFAHSGDNSNLERQFERDKDDIRDLGVPLETLESPGQAGNNQNLRYRIPRGEYELPAD
                     ISFSPEETTLLNLAAMVWREGSLSGESRRAIIKLRSLGVVTTEPVLGYAPRVRVRDAA
                     FEPLSSALEKHVIVQFSYLKPGEGAPRLRTVAPLALVQHQGRWHLYAQEPESGTTKTF
                     LLRRIVSPVTITTKTFPPPAADQSERALAELEEVWRSHTATVQVEPGSDAATRLGKRR
                     GTVAAASGDLELHYSDANILADELAGFGPEVLVLSPPELRDAVRDRLMRTAADHG"
     gene            complement(2883..3839)
                     /locus_tag="PLW15_07070"
     CDS             complement(2883..3839)
                     /locus_tag="PLW15_07070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT34189.1"
                     /translation="MRKLPAILATAGLLVSLSACTGAPFSSACTPAFPSGANSALVTA
                     DGAVGTAPKTTFPTPLVAKSTEVTELVKGDGAIVAPGDVVDFQVTALLGSTGEVLTAS
                     SYDKTKPVRRTVGGSDALGQMLQCASVGSRVASAASFESIFPDVDPSTAGVKATDTIV
                     LVLDAQRVFPGRATGADQLAPAGFPSVVLAPNGQPGFTFGSSTPREDLAWAALKQGNG
                     AVVKKGDVVVAQVTGVVWGASTVFTSTWDDGNPVNVTAASTADDPNGVPPGWAKALIG
                     QKVGSQVIVSIPPSEGYASGSAPTGVSDGDTIVLVFDILGIL"
     gene            complement(3889..4890)
                     /locus_tag="PLW15_07075"
     CDS             complement(3889..4890)
                     /locus_tag="PLW15_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOT34190.1"
                     /translation="MSDAIRRQSGPFRAGDRVQLTGPKGRLNTVTLEVGGEFHTHHGV
                     LSHDLIIGAPDASVVTSSNGIEYLALRPLLTDFVMSMPRGAAIIYPKDAAQILGQADI
                     FPGATVVEAGVGSGALSLWLLRAIGPAGRLVSFERRQEFADVAQGNVGAFLGAQPENW
                     SVVVGDLQDALPATLEAGSVDRIVLDMLAPWECLDVCATALTPGGVLICYIATVTQLS
                     RVAEAIRATGEFTDPEPSETLVRTWHVEGLAVRPDHRMIGHTGFLLTARRLAPGTVVP
                     ALKRRASKSDYTDEDVEIWTPGALGDRSASDKALRKAAREAQAAARAASQEATPARE"
     gene            complement(4887..5558)
                     /locus_tag="PLW15_07080"
     CDS             complement(4887..5558)
                     /locus_tag="PLW15_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT34191.1"
                     /translation="MTHSHPAAVLWDMDGTLVNTEPYWIAAETELIHDFGGDWSHEEA
                     LQLVGSGLWESARIIQAKGVTLTEDEIIDRLTDRVLEQLVEFGIPWRPGARELLTEIR
                     EAGVATALVTMSIGRMAHHVADRLGFHGFDAVVSGDDVVASKPHPEPYLKGAEALGVD
                     PRYCVALEDSVPGIASARAAGTTVIGIPFIVPIEEGSAHVLWPTLEGRTLADLGRVLA
                     TSAAM"
     gene            5625..>6467
                     /locus_tag="PLW15_07085"
     CDS             5625..>6467
                     /locus_tag="PLW15_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOT34192.1"
                     /translation="MAGSKFAGERLLVVAFEGWNDAGEAASGAVRTLKEALDLYPIAE
                     VDPEDYFDYQFNRPSVAADEDGNRQIVWPAVTIYGPTQPNSGRQSEVYLLLGTEPSRG
                     WKTFTREILDTVDENGITSIIFLGAMLADVPHSRPISVFTSSENAAVRSELAIERSGY
                     EGPVGILSVLADAAEKIGITTMSIWASVPHYVHNAPSPKATLAIIDKLEEIIGIDIPR
                     GELESEAAAWESGIDALAGDDDDMASYIEQLEQARDTVDSPEASGEAIAQEFERYLRR
                     GGDKP"
BASE COUNT         1074 a         2124 c         2240 g         1029 t
ORIGIN      
        1 gcgttgcgct gctcggcgat ctggttgatc ggcactcgca gggagtcgat cacgtagggg
       61 cgaggaagta acccgccacg actgccacga gaatggcgat cgccgagatg acgagacgct
      121 tgcgcaattc acggaggtgt tcgccgagcg acatgcgctc gtcgcccttg ttgcgccacc
      181 gcccggagcg ggtggccacg cgaactaggg cttcggcttg gtggccttgg gcgcggcctt
      241 gggggccgtg ctcctggccg tcgtcgtctt cgctgtgctg gccttcggag cctcgggctc
      301 ctcgtcctcg acaggcgccg cgtcatcgtc cttcatggtc ttgacctcgc tacggaagat
      361 gcgcatcgac tgcccgatgc tgcgcgcgag tcccggaagc ttcggcgctc cgaacaagag
      421 gagcacgact gccagaatga cgatcaggtg gggccagccg aatgcattgc cgaacatggt
      481 caaactccct cggggatcgt gtcagttcat gatacccagt cggcgggcca ctcgcgctga
      541 gcggcagccg ggccggtgat gcggcgggca cgtgccatcc gtcgctcatg tcgttggcgt
      601 cgcagctcgg cgcgatgcgc ccgccgagcc gcctctcggt cacggatgtc gcgcacctcg
      661 gcaagcacgg cgatctcggg cttcacgagc accgggtcgt ccacctcgag cagcgcgctg
      721 cgctcggcca ggtcggccag gtcatcggtc agggtgagga acttgcggaa caaccaccac
      781 gcgaacaggc cgagcatgac gagcagaccg agcacgaggc cggtccagat cagcagccag
      841 gcccaccagg ggatgatctc gacaggctcg atcaccggta ccggtccgcc ccagcagccg
      901 cccactcggc gacgacggcg cgggcctccg gcggcgcgac cactgtcgcg acccccggca
      961 tgctcgcgat cagccgcttg aggccgtggt agtgcgagac gcgcacggtc gtgcgcactc
     1021 tcccgtcgac gatcgtcgag gtcgcgccgt cgggcacgta gtcggccagc agggcctcgg
     1081 caccggccgc cacatcgatg gtcaccagga ggtcgtcggc cgagccctcg aacagggtgt
     1141 ccggcagggt gacatccgtc gccccgtggg ttatcggctc gtcggtgaca atcggatccg
     1201 agatgcggtc gaggcggaat gtgcgcacgg cctcgcgcag gtggcaccag ccgcgcaggt
     1261 accagtccgc gtcgaccgat tcgacgcgca gcgggtccac gtgacggttc tcgtgctcgc
     1321 cgcgcgagtt gaggtagtcg aacttgagct gcaccccgtg ctcgacggcc tcgcggatca
     1381 ggcgcagcgt gtcatccgtc tcgctggctt cgaccgcgac ctggctggga accgccgacg
     1441 cgccgcggga cagcttggac tgcaacgagg cgatcgcggc gcggtcggcg tgctcgggca
     1501 acgccgagag gtactgcagg ccggcgatca gcgctgcggc ctcccgggcc gagaaccgcg
     1561 gcgaatcatc gatggcaacg aggttcgtga gcacgatctc gtcgttgaac tcgaagtcgt
     1621 cccacgagat gtcaaacagg tcgccgtgct ggtacgacgc ggtctcgccg gggatgcccg
     1681 acacgctgat gaggcgcacc gcgtcgcgga tctgctcctc gggcacccca aaatgggctg
     1741 cggcctcggc gacgctcaca cggtcgtgat ccatcaggta cggcacgagg gagagcagga
     1801 acgcgagctt gtcctgcgcc ttctggggct gcgcgcgctc agccatggtc tgccgccgtt
     1861 cgcatcagcc ggtcgcgcac ggcgtcccgc agctcgggcg gggatagcac aagcacctcg
     1921 ggtccgaatc cggcgagctc gtcggcgagg atgttggcgt cggagtagtg cagttcgaga
     1981 tccccggatg ccgcggcgac agtaccccgt cgcttgccga ggcgagtggc ggcatccgat
     2041 cccggctcga cctgaacggt agcggtgtgg ctcctccaga cctcctcgag ctccgcgagg
     2101 gcacgctcgg actggtcggc tgcgggcggc gggaaggtct tcgtggtgat cgtcacgggg
     2161 ctgacgatgc gccgcagcag gaacgtcttc gtcgtgcctg attcgggttc ctgagcatag
     2221 agatgccatc gaccctgatg ctgcaccagc gcgagcgggg cgacggtgcg aagacgcggt
     2281 gcgccctccc ccggcttgag gtaggagaac tgcacgatga cgtgcttctc gagggccgag
     2341 ctcaatggct cgaacgcggc atcccgcacc cgcacccgcg gtgcgtagcc cagcacgggc
     2401 tctgtggtga cgacgccgag cgaacggagc ttgatgatcg cgcgccggga ctcccccgaa
     2461 agcgatccct cccgccacac catggccgcg aggttgagca gcgtggtctc ctccggcgag
     2521 aagctgatgt cagccgggag ttcgtactcg cctctcggga tgcggtagcg aagattctgg
     2581 ttattgcccg cctgaccggg actttccagg gtctccagcg gcactccgag atcgcggata
     2641 tcgtccttgt cgcgctcgaa ctgacgctcg aggttcgagt tgtcgcccga gtgcgcgaac
     2701 ttctgccggt agccctgcac cgtggagagg atctcgttct tggtgagccc ggtatcggtc
     2761 gccaagagcg ccaggacgag actgaagagt ctctcctcga cgggaatcct tgcggcggag
     2821 ctggctgggg gcacgagaga aatcctaatg ttcgtgacac ccggccgtgt ctggtgctga
     2881 ctctagagga tccccagaat atcgaacacg agcacgatcg tgtcgccgtc gctgacgccc
     2941 gtcggagcgc tgcccgacgc atagccctca ctcggcggga tcgagacgat gacctgcgaa
     3001 ccgaccttct ggccgatcag ggccttcgcc catcccgggg gaaccccgtt gggatcatcg
     3061 gctgtgctcg ccgcggtgac gttgacggga tttccgtcgt cccacgtcga cgtgaagacc
     3121 gtgcttgcgc cccacacgac gccggtgacc tgcgcaacga cgacgtcgcc cttcttcacc
     3181 actgcgccgt tgccctgctt gagggctgcc caggccaggt cctcacgggg ggtggagctg
     3241 ccgaacgtga agccgggctg gccgttgggg gccagcacga ccgacgggaa gccggcgggg
     3301 gccagctggt cggcaccggt ggcgcgcccc gggaagacgc gctgcgcgtc gagcacgagc
     3361 acgatcgtgt cggtggcctt cactccggcg gtgctcggat ccacatcggg gaagatgctc
     3421 tcgaagctcg cggcagaagc cacacgggag cccacagatg cgcactgcag catctggccg
     3481 agggcgtcgc taccgccaac ggtgcgcctg acgggcttgg tcttgtcgta actcgaggcc
     3541 gtgagcacct cgccagtcga tcccagcagc gcggtgacct ggaagtcaac gacatcgccg
     3601 ggggcgacga ttgcgccgtc acccttgacg agctcggtca cctcggtcga cttcgcgacg
     3661 agcggtgtgg ggaaggtcgt cttcggggcg gtaccgacag cgccatccgc cgtcacgaga
     3721 gccgagttgg caccgctggg gaaggccgga gtgcacgcgg aactgaacgg cgctccggtg
     3781 caggcggaca gggagaccag caggcctgcg gtcgcgagaa tagccgggag cttgcgcacg
     3841 aacactcgtt tcttgagggt gtcttgaggg gtggataccg cgaatactct actcgcgggc
     3901 aggcgtggct tcctgcgaag cagcccgggc tgcggcctgt gcctcgcggg cggccttgcg
     3961 cagcgccttg tcactcgcac ttcggtcgcc gagtgctccg ggcgtccaga tctcgacgtc
     4021 ctcatcggtg tagtcggact tggatgcgcg ccgtttgaga gcgggcacga cagtgcccgg
     4081 cgcaagccgc cgtgcagtca gcaggaaccc cgtgtgaccg atcatgcggt ggtcggggcg
     4141 caccgccagc ccctcgacgt gccaggtgcg cacgagcgtc tcgctcggct cgggatcggt
     4201 gaactcaccg gtcgcccgaa tagcctcggc gacccgcgag agctgagtga ccgtggcgat
     4261 gtagcagatg agcactccgc ccggggtcag agcggtcgcg cagacgtcca ggcattccca
     4321 gggcgcgagc atgtcgagca cgatgcggtc gaccgagccg gcctcgaggg tggccgggag
     4381 cgcatcctgc aggtcaccga cgacgaccga ccagttctcg ggctgggctc ccaggaaggc
     4441 acccacgttg ccctgtgcca cgtcagcgaa ctcctgccgg cgctcgaatg acacgagcct
     4501 gcccgccggg ccgatggccc gcaggagcca cagcgagagt gctcccgagc ccaccccggc
     4561 ttcgacgacg gttgcgccgg ggaagatgtc ggcctggccg agtatctgag cggcatcctt
     4621 cgggtagatg atggcggctc cgcggggcat cgacatgacg aagtcggtga ggagggggcg
     4681 gagggcgagg tactcgatgc cgttgctcga ggtgacgacc gaggcatccg gagcaccgat
     4741 gatgaggtcg tggctgagaa cgccgtgatg tgtgtggaac tcgccgccca cctcaagcgt
     4801 cacggtgttg agccggccct tggggccggt cagctgcacg cggtcgccgg ctcggaacgg
     4861 cccgctctgg cggcgaatcg cgtcgctcac atcgccgccg aggtcgcgag aaccctgccc
     4921 aggtcggcca gggtgcgtcc ttcgagggtc ggccacagca cgtgcgcaga accctcctcg
     4981 atgggaacga tgaaggggat gccgatgacg gtcgtgcccg ctgcgcgagc agatgcgatg
     5041 ccgggcaccg agtcctcgag tgccacgcag tagcgggggt cgacgcccag cgcctcagcg
     5101 cccttgagat acggctcggg atgcggcttg ctcgcgacca cgtcgtctcc ggacaccacg
     5161 gcgtcgaagc cgtggaagcc gagtcgatcg gcgacatgat gggccatccg gccgatcgac
     5221 atcgtgacga gggcggtcgc gacaccagcc tcccgaatct cggtcagcag ctcgcgtgct
     5281 cccgggcgcc acgggattcc gaactcgacg agttgctcga gtacccggtc ggtgagacgg
     5341 tcgatgatct cgtcctcggt gagggtcaca cccttggcct ggatgatccg agcggactcc
     5401 cagaggcccg agccgaccag ttgcagcgct tcctcatgac tccagtcacc cccgaagtcg
     5461 tggatgagct ccgtctcggc ggcgatccag tagggctcgg tgttgacgag tgttccgtcc
     5521 atatcccaga ggacggcggc ggggtggctg tgggtcacaa cccacaatcg tagttctatc
     5581 cttgtcacag ggccgccgac ggcggagccc cggcagggag agacgtggca ggcagcaaat
     5641 tcgcaggcga acggctactc gtcgtcgcct tcgagggctg gaacgacgct ggcgaggccg
     5701 cgagcggcgc ggtacgcact ctcaaggagg ctctggacct ctaccccatt gcggaggtgg
     5761 accccgagga ctacttcgac taccagttca acagaccgtc ggtagcggcg gacgaggacg
     5821 gcaatcggca gatcgtctgg cccgccgtca ccatctacgg accgacccag cccaactcgg
     5881 ggcgccagtc cgaggtgtat ctgctactcg gcaccgagcc gtcgcgcggc tggaagacct
     5941 tcacccggga aatactcgac acggtcgacg agaacggcat cacctcgatc atcttcctcg
     6001 gagcgatgct tgccgacgtg ccgcactcac ggccgatctc ggtgttcacg agcagcgaga
     6061 acgccgcagt gcgctccgaa ctcgccatcg aacgcagcgg ctacgagggt ccggtgggaa
     6121 ttctctccgt gctggcggat gccgcggaga agatcggcat cacgacgatg tcgatctggg
     6181 cctctgtgcc ccactacgtt cacaatgctc cctcgcccaa ggcgacgctc gcgatcatcg
     6241 acaagctcga ggagatcatc ggcatcgaca tcccccgcgg ggagctcgag agcgaggccg
     6301 cggcctggga gagcggcatc gacgcactcg ccggcgacga tgacgacatg gccagctaca
     6361 tcgagcagct cgagcaggcg cgtgacactg tcgactcccc cgaggccagc ggagaggcga
     6421 tcgcgcagga gttcgagcga tacctccgtc gtggcggcga caagccg
//
LOCUS       DAOSOU010000109         6441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12129_length_6441_cov_5.469151, whole genome shotgun sequence.
ACCESSION   DAOSOU010000109 DAOSOU010000000
VERSION     DAOSOU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12129_length_6441_cov_5.469151"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PLW15_07090"
     CDS             <1..1260
                     /locus_tag="PLW15_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT34193.1"
                     /translation="AGVDISGFVYLGLVAVLLLMSATQLAEGLSALRRNPLPPPSATP
                     APPATAIVAAYLPNEAATILETLRSVLSQKYDGGLQVILAYNSPVSLPVEDELRALAA
                     QEPRLELLRVAYSRSKADNVNAALAIVTGEFVGIFDADHHPMVGSFERAWRWFADGAD
                     VVQGHCVVRNGDESGIARLVAVEFEQIYAVSHPGRQRLHHFGIFGGSNGYWRTQLLRE
                     TRLRSQFLTEDIDSSIRAVRMGARIVNDPGLISRELAPVNFRALWKQRMRWAQGWFEV
                     SLRHGHSAISPSGLSLRQRYGLWMLLGWRELFPWIASLMWPTLAFFIWRDNGIDLANA
                     PQVFWLTTAFTLTTGPILIAFAYKLGAPEIREHTSWWWQYVFVSVIAYSELKNIIVRV
                     AQIRHILRQDEWVVTPRGHVDRTSTRT"
     gene            complement(1257..1769)
                     /locus_tag="PLW15_07095"
     CDS             complement(1257..1769)
                     /locus_tag="PLW15_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOT34194.1"
                     /translation="MTEVQPYTVERSYDGFEVRRYPDHQLVEVDVDGSFFEAGNRGFR
                     PLIGFISGNNEARQQIAMTAPVIQSPRPAGGQTVSFVMPETMDAASVPTPTNSAVRSR
                     TVAGGLVAARRFSGGSSTPRYEDNAADLLAALVLEGLTPDGDVYFARYDPPWKPGFLK
                     RNEALVRLAD"
     gene            complement(1787..2419)
                     /locus_tag="PLW15_07100"
     CDS             complement(1787..2419)
                     /locus_tag="PLW15_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT34195.1"
                     /translation="MTDIAMFPLGSVLFPHMPLLLKVFEDRYLIMLSQILQDEPSEFG
                     VALIERGQEVGGGEQRFPVATVAKITQMDATEGFVVLVAEGGRRVEVDQWLDDDPYPR
                     ASVTEMAELVWTDDLQPLRDRAEESVRRIITLASEFADQTWPADITLADDPVAAAWQL
                     AAIAPFGPLDQVTLLRAASLQQLLENIIEFAVGAELTLRAPWADDEEFPD"
     gene            2465..2812
                     /locus_tag="PLW15_07105"
     CDS             2465..2812
                     /locus_tag="PLW15_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34196.1"
                     /translation="MATRAKSRRWLPIAGIAAASLIVVGAFAGPSLFASAGQTTAAEV
                     TRSAPQFAKGSAQAPAKQPASAATSGTVTPETPAGGSSSVFVPLPCPDGSTPVDSADA
                     ALPCETPSGYPGG"
     gene            2881..3651
                     /locus_tag="PLW15_07110"
     CDS             2881..3651
                     /locus_tag="PLW15_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34197.1"
                     /translation="MAKSDKKVVRVESTPASKAEEARESNWKPTPEAKSKAIGFRVIA
                     GVLWLLAIGTEAFAIFWVLRQNPVNLILLIVLLVVIGGLAVGGSLLWKQANRLDPASR
                     TDKFRFFVQNQLGAIITIIAFLPLIIMIFLNKDMDGKQKGIAGAIGIVLLLVAGYFGT
                     SINPPSTEQYTAETEQVVEITGQNLVFWTKSGNVFHLCQDASAVNLDSADNTIYSGTV
                     AEAHAAGKPRLTLQVEQEKKQCGFDTAAEVTPEPTPTP"
     assembly_gap    3796..3895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3896..4659)
                     /locus_tag="PLW15_07115"
     CDS             complement(<3896..4659)
                     /locus_tag="PLW15_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34198.1"
                     /translation="MDDMTALRTALAVTGLSIALLAIAAPANAAGPSLPDGDTLIAAS
                     CLGGTDATIFDVNSTTGAATALGSGNAGYECAYQASWDASSKTLYGTIWDADSVLVKW
                     DTLTGALVEVGPVLDGVTPVDVDTLVIGLDGAAYVLDFNELYRVDLATGAATDLGALA
                     ALTTNAYGAAVDPTTGILYLLAEDGDLFTLDPVAVTATFVASWSFSAEAENVYGLAID
                     SAGIAWVVEYPGDTVYSALYSTPLATFGVAPVLSGD"
     gene            complement(4735..5103)
                     /locus_tag="PLW15_07120"
     CDS             complement(4735..5103)
                     /locus_tag="PLW15_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOT34199.1"
                     /translation="MTVEFLTLEDLLALAADLGVPAVRDLGLLDAAAKRPQTSLYGRD
                     AYTGIHEKAAVLLESLTRNHALIEGNKRLGWLATYVFYALNGVQLEAPDDAAYDLVIA
                     VSTGALAFTESAHRLEGWCR"
     gene            complement(5100..5303)
                     /locus_tag="PLW15_07125"
     CDS             complement(5100..5303)
                     /locus_tag="PLW15_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOT34200.1"
                     /translation="MAMTLRLTDEQERALALLADAQGISKQEATTRAITEAAARHVRT
                     ERVSELSALGRERYAALLDRLAQ"
     gene            complement(5452..6330)
                     /locus_tag="PLW15_07130"
     CDS             complement(5452..6330)
                     /locus_tag="PLW15_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34201.1"
                     /translation="MTEASSTPAPTRRRGLPWWAWALIALGVVVILIVPVMTVVGFFA
                     YARHSVSETSPDQPFISGPRQQPAAKTPLACPEACFTIDAATLMAVSADDVSQLSIKD
                     ELYAVGEIGQSTVSEVAPRVGEQWLSVDGDEECSFVPVNAPYIPVGPDSSSEDPISWV
                     QTWETGDEMTDISARVFATTDAASAFMSDIHQRVAACPWQDLNVPAAGGLDTSLLQIT
                     AQAAIDVPGEVAAVGWVREGDPGPRWRAYVWELQRGNLVVQMRVLTDGRIVEEQVATY
                     AEFIAERLGRLQAGTP"
BASE COUNT         1081 a         2132 c         2092 g         1036 t
ORIGIN      
        1 gcgggcgtgg atatctcggg cttcgtgtat ctcggtctgg tggccgtact gctgctcatg
       61 tcggcaactc agctcgctga gggtctcagc gccctgagga ggaatcccct gccaccgccc
      121 agtgcaacgc ccgcgccgcc agcgacggcc atcgttgccg cctatctgcc gaacgaggcc
      181 gccacgattc tggagaccct gcgctccgtg ctgtcgcaga agtacgacgg cggccttcag
      241 gtgatcctcg cctacaactc gcccgtgagc cttccggtgg aggacgagct ccgggccctc
      301 gcggcccagg aaccgcgcct cgaactgcta cgggtcgcct acagccgatc gaaggccgac
      361 aacgtcaacg cggcactggc catcgtgacc ggcgagttcg tgggaatctt cgacgccgac
      421 caccacccga tggtcggatc cttcgagcgc gcgtggcgct ggttcgccga cggcgccgac
      481 gtcgtgcagg ggcactgcgt cgtgcgtaac ggtgacgagt cgggtatcgc ccgccttgtc
      541 gcggtcgagt tcgagcagat ctacgccgtc agccacccgg ggcgccagcg actgcaccac
      601 ttcggcatct tcggcggctc gaacggatac tggcgcaccc agcttctgcg cgaaacccgc
      661 cttcgctcgc agttcctgac cgaggacatc gactcgtcca tccgcgcggt gcggatgggg
      721 gcacgcatcg tcaacgaccc gggcctgatc agtcgcgagc tcgcgccggt gaacttccgc
      781 gcgctgtgga agcagcggat gcgatgggcc cagggatggt tcgaggtgag cctgcgccac
      841 ggccactccg ccatctcgcc cagcggcctg agcctgcgcc agcgctacgg cctgtggatg
      901 ctgctgggct ggcgcgaact cttcccctgg atcgcctcgc tgatgtggcc gaccctcgcg
      961 ttcttcatct ggcgcgacaa cggaatcgat ctggccaatg ctccgcaggt gttctggctc
     1021 acgacggcgt tcacgctgac gacaggcccg atcctgatcg ccttcgccta caagctcggc
     1081 gctccggaga tccgcgagca cacgtcatgg tggtggcagt acgtcttcgt gtcggtgatc
     1141 gcgtactccg agctgaagaa catcatcgtg cgcgtggcgc agatccggca catcctccgc
     1201 caggacgagt gggtcgtgac cccccgcggt cacgtcgatc gaacgtctac ccgcacctag
     1261 tcggcgagcc tgaccagcgc ctcgttgcgc ttgagaaatc ccggcttcca cggcggatcg
     1321 tagcgcgcga agtacacgtc gccgtcgggc gtgagaccct cgagaacgag cgccgcgagg
     1381 aggtcagccg cgttgtcctc gtagcgcggc gtcgacgagc cgccgctgaa cctgcgcgcc
     1441 gcgacgagac cgccagccac ggtgcgtgag cgcacggcgg agttcgtcgg agtgggcact
     1501 gaggcggcat ccatcgtctc gggcatcacg aagctgaccg tctgaccgcc agcgggccgc
     1561 gggctctgga tgaccggggc cgtcatggcg atctgctgcc gggcctcgtt gttgccgctg
     1621 atgaacccga tgaggggacg gaagccccgg ttgccggcct cgaagaacga gccatcgacg
     1681 tcgacctcga cgagttggtg gtctggatag cgacgaacct cgaacccgtc gtagctgcgc
     1741 tcgacggtgt acggctgcac ttcggtcatg gccgcagtgt acgtcgtcag tccgggaatt
     1801 cctcgtcgtc ggcccacggc gctcgcaggg tgagctccgc ccccacggcg aactcgatga
     1861 tgttctccag aagctgctgg aggctcgccg ctctgagcag cgtcacctgg tcaagcggac
     1921 cgaaaggggc gatggctgcg agctgccagg cggccgcgac ggggtcgtcg gcgagggtga
     1981 tgtctgctgg ccaggtctgg tcggcgaact cgctcgccag cgtgatgatg cggcgcaccg
     2041 actcctcggc gcgatcccgc aggggctgga ggtcgtcagt ccagacgagc tcggccatct
     2101 cggtgaccga tgcccgcggg taggggtcgt cgtccagcca ctggtcgacc tccacccgac
     2161 gcccgccctc cgcgaccagc acgacgaagc cctcggtcgc atccatctgg gtgatcttgg
     2221 cgacggtggc gacggggaag cgctgctcgc cgccgccgac ctcctggccg cgctcgatga
     2281 gtgcaacccc gaattcggat ggctcgtcct gcaggatctg cgacagcatg atgagatagc
     2341 ggtcctcgaa gaccttcagc agcagcggca tgtgcgggaa cagcaccgag ccgagcggga
     2401 acatcgcgat gtcggtcacg gtgtccagta ttgtcgcagg gtgggcagga gacgaggcag
     2461 gcacgtggcg acacgggcca agtcccgcag atggcttccg atcgcgggga tcgccgccgc
     2521 atccctgatc gtcgtaggcg cttttgccgg cccgtcactg ttcgcctccg caggccagac
     2581 gaccgctgcc gaggtgaccc gcagcgcgcc gcagttcgcc aagggctccg cgcaggcgcc
     2641 tgcgaaacag ccggcgagcg cggcgacatc gggcacggtc acccccgaga ctccggctgg
     2701 tggctccagc tcggtcttcg tgccgctgcc gtgtcctgac ggctcgaccc cggtcgattc
     2761 ggccgatgcg gcgctgccgt gcgagacgcc ctcgggatat cccggcggct gatcccgtcg
     2821 ccgcgtcacg agagcccgcc agcgtcgata tgctcgtggc aacaagtctt ggagggcatc
     2881 atggcgaaga gtgacaagaa ggtcgtgcgg gtcgaatcca cgccggcgag caaggccgaa
     2941 gaggcgcgcg agtcgaactg gaagccaacc cctgaagcaa agagcaaggc gatcggattc
     3001 cgcgtgatcg ccggcgtgct ctggctgctc gcgatcggca ccgaggcctt cgccatcttc
     3061 tgggtgctgc gccagaatcc ggtcaacctg atcctgctga tcgtgcttct cgtcgtgatc
     3121 ggcggtctgg cggtcggcgg atccctgctg tggaagcagg ccaaccgcct cgaccccgcc
     3181 tcacgcaccg acaagttccg gttcttcgtt cagaaccagc tgggcgccat catcacgatc
     3241 atcgccttcc tgccgctgat catcatgatc ttcctcaaca aggacatgga cggaaagcag
     3301 aagggcatcg ccggcgcgat cggcatcgtg cttctgctgg tggcgggata cttcggcacc
     3361 tcgatcaacc cgccgtcgac cgagcagtac accgctgaga ccgagcaggt cgtcgagatc
     3421 acgggccaga acctcgtgtt ctggaccaag tcgggcaacg tattccacct ctgccaggat
     3481 gcctcggccg tcaacctcga ctccgctgac aacaccattt actccggaac agtcgccgag
     3541 gcccacgccg cgggcaagcc gcgcctcacg ctgcaggtcg agcaggagaa gaagcagtgc
     3601 ggtttcgaca ctgcggccga ggtgaccccg gagccgaccc cgacgccgta acgcccggtc
     3661 gcgcgatcag ccgaacgcgc cgaggatcag cccagcgacg gtgagcgaca ccacgtcgcc
     3721 gcccacttcg atgatccaca cacgcagacc ctgtccaccg aagagccggt gtgagaggga
     3781 gcttgctgcg gcaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgtc
     3901 acccgagagc acgggcgcga cgccgaaggt agccagcggc gtgctgtaca gggcggagta
     3961 gaccgtgtct ccggggtact ccaccaccca ggcgatacct gcgctgtcga tggcgagacc
     4021 atagacgttc tccgcctcgg cggagaacga ccagctggcg acgaacgtcg cggtcacggc
     4081 cacggggtcg agggtgaaga ggtcaccgtc ctcagcgagc aggtacagga tgcccgtggt
     4141 ggggtcaact gcggctccgt aggcgttggt cgtaagggcg gccagggccc cgaggtcggt
     4201 cgccgcaccg gtagccaggt cgacccgata cagctcgttg aagtcgagga cataggccgc
     4261 cccatccaga ccgatcacca aggtgtcgac gtcaaccggg gtgacgccgt cgagtaccgg
     4321 ccccacttcg accagggcgc ccgtgagggt gtcccacttg accagcaccg agtcggcatc
     4381 ccagatcgtg ccgtacagcg tcttggacga cgcatcccac gacgcctggt aagcgcattc
     4441 gtagcccgcg ttgccggagc cgagcgcggt ggcagcacca gtggtcgaat tcacgtcgaa
     4501 gatcgtcgca tcggtgccgc cgaggcacga agcggcgatc agcgtgtcgc cgtcaggcag
     4561 gctggggccg gccgcgttgg ctggggccgc gatggcgagg agtgcgatcg aaaggccggt
     4621 gacggcgagg gcggtgcgca gggcggtcat gtcgtccatt cgggcaggga tcagagactt
     4681 tccacaccat acagcatcct ctcaggttgc tgcaacagtc cgctcggggt cccgtcaacg
     4741 gcaccagccc tcgagccggt gcgccgattc ggtgaaggcc agcgcacccg tcgacaccgc
     4801 gatgaccaga tcgtacgcgg catcgtcagg agcctcgagc tgaacgccgt tgagcgcgta
     4861 gaacacgtag gtcgcgagcc aacccagacg cttgttgccc tcgatgagcg catggttgcg
     4921 ggtgagggac tcgaggagca cagccgcctt ctcatggatg ccggtgtagg cgtcgcgccc
     4981 gtacagactg gtctgggggc gcttcgccgc ggcatccaga aggcccagat ctcgcaccgc
     5041 cggaacgccg agatcagcgg cgagcgcgag gaggtcttcg agagtgagga actccaccgt
     5101 cactgagcga gacggtcgag cagtgccgca taccgctcgc ggccgagcgc ggacagctcg
     5161 gacacgcgct ccgtgcgcac gtgccgggcg gcggcctccg tgatcgcgcg agtggtcgcc
     5221 tcctgcttgc tgattccctg agcgtccgcg aggagagcga gggcgcgttc ctgctcgtcg
     5281 gtcagcctca gagtcattgc catggtggcg atggtatcac tgtggtatca gctgttggac
     5341 ctgaactcga ccacgtcgcc gtccacatct tcgggaggcc accatgtgag gccgccgccg
     5401 cgcagggcgg gcatgaaacc tgggttcggc cctgaaggga tcgagagcgg gtcagggcgt
     5461 tccagcttga aggcgaccca atcgctccgc gatgaactcg gcatacgtcg ccacctgttc
     5521 ctcaacgatg cgcccgtcgg tgagcactct catctgaacg acgagattgc cgcgctgcag
     5581 ctcccagacg tacgcgcgcc agcggggccc gggatctccc tcgcgcaccc acccaaccgc
     5641 agcaacctct ccgggcacgt cgatcgcagc ctgcgccgtg atctgcaaca ggctcgtgtc
     5701 gaggccgccc gcagcgggta cattcaggtc ctgccacggg cacgcggcaa ctcgctggtg
     5761 gatgtcactc atgaacgcac tagcggcgtc ggttgtcgcg aagacacggg ccgagatgtc
     5821 agtcatctcg tcccctgtct cccacgtctg aacccagctg atcggatctt cagacgaact
     5881 gtccggtccc acggggatgt aaggtgcatt caccggtacg aacgaacatt cctcgtcgcc
     5941 gtccactgag agccactgtt cccccacgcg cggtgccacc tcagatacgg tgctctggcc
     6001 gatctccccc acggcgtaca gctcgtcctt gatcgacagc tgggacacgt cgtcggccga
     6061 gaccgccatg agggtcgccg cgtcaatggt gaagcatgct tcagggcatg ccagtggcgt
     6121 cttggccgcc ggctgttgcc gagggccgct gatgaacggt tggtccggac tcgtctcgct
     6181 cacgctatgc cgcgcgtacg cgaagaagcc cacaaccgtc atgaccggga cgatgaggat
     6241 gacgaccacc ccgagcgcga tcaacgccca cgcccaccag ggcaagccac gtcgtctggt
     6301 tggcgcggga gtcgagcttg cctcagtcat gtcactatct tgcttcaccg gaagcgggcc
     6361 tgggcggccc accaataacg atgtcgtaaa gggtctggcc cgcgtctcta gccgccccct
     6421 cggcgcaagt cgggccgaca g
//
LOCUS       DAOSOU010000110         6429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12164_length_6429_cov_7.203640, whole genome shotgun sequence.
ACCESSION   DAOSOU010000110 DAOSOU010000000
VERSION     DAOSOU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12164_length_6429_cov_7.203640"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLW15_07135"
     CDS             complement(<1..448)
                     /locus_tag="PLW15_07135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HOT34202.1"
                     /translation="MPLRIQDVSADAILLAGGARAILLQVANPGVGVGVAEHSDFANR
                     PLDRLRGTLTYLYVTVYGTPDEAKAVARRVGGAHKPVPGANDVELQLWVAATLYETAM
                     RVRELVYGPLDDSDAESLLADYAVVGTALGVPRALWPADRAAFDAYW"
     gene            634..1797
                     /gene="sucC"
                     /locus_tag="PLW15_07140"
     CDS             634..1797
                     /gene="sucC"
                     /locus_tag="PLW15_07140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOT34203.1"
                     /translation="MDLYEYQARDLFESYGVPVLAGIIADTPAEAKAAAEKIGGTVVV
                     KAQVKAGGRGKAGGVKVVHNPDDAATAAEAILGLVIKGLTVKRVMVAQGARIAQEFYF
                     SILLDRANRSYLSLCSVEGGMEIEELAVERPDALAKVEVNPILGIDLEKAKEIALAGG
                     FPADLVEKVAPVIVKLYEVYTGEDATLVEVNPLVLTEDGQVLALDGKVTLDENAGFRH
                     PNHEALEDKDAADPLEAKAKANDLNYVKLDGEVGIIGNGAGLVMSTLDVVSYAGERHG
                     GVKPANFLDIGGGASAEVMANGLDVILGDAQVKSVFVNVFGGITACDAVANGIVQALE
                     ILGDSATKPLVVRLDGNKVEEGRAILRAANNPLVTLADTMDDGADKAAELASR"
     gene            1841..2728
                     /gene="sucD"
                     /locus_tag="PLW15_07145"
     CDS             1841..2728
                     /gene="sucD"
                     /locus_tag="PLW15_07145"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOT34204.1"
                     /translation="MSIFLNKSNKVIVQGITGGEGTKHTALMLKAGTQVVGGVNARKA
                     GTTVEHEGGVILPVFGTVKEAMEATGADTSIVFVPPAFAKDAVVEAIEAEIPLVVVIT
                     EGIPVQDTAEFWALAKAMGTTQIIGPNCPGIITPGESLVGITPATITGKGPIGLVSKS
                     GTLTYQMMYELRDLGFSTAIGIGGDPIVGTTHIDALRAFQDDPETKAIVMIGEIGGDA
                     EERAADFIREYVTKPVVGYVAGFTAPEGKTMGHAGAIVSAGAGTAQGKKEALEAAGVK
                     VGKTPSETAALMREVLAAG"
     gene            2738..4279
                     /locus_tag="PLW15_07150"
     CDS             2738..4279
                     /locus_tag="PLW15_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34205.1"
                     /translation="MTSRRLPLVAAIAALSLAALSGCQFLPSIVGPTETGPTGPAPTD
                     VAGFYSQTVTWTDCSEDLECATVHAPTNWNSPSDGAIELALVKHKATGTKKGSLLLNP
                     GGPGGSGWDYVYYSGASSATAAVSEAYDIVGWDPRGVGKSTPVTCYTDPKQTDEVLYG
                     TFDSPYGTQGWIDELTVEEEKFAAACQQNTGELLANLDTASNAKDMDMIRALVGDEKL
                     NYLGYSYGTYFGAVYAELFPDKVGRLVLDGALDPLLSDFESLKVQMAGFDSAFRAYMQ
                     FCLQGSDCPFSGAVDTALGQARTVLETVDAQGLVNSDGRVLDSATLGTAIALNLYSED
                     YWPDMTQMFAELSTGDPTLAFVNADYYNSRNADGSYSSNSFEVYTAATCVDGDFADDP
                     ATTLDRLAEIDAAAPILGKFFAYDDFAVLDTACSNWPVPRADLPMSFEAKGAPPILVI
                     GTTNDPATPYAWAQALASQLSSGVMITHKGEGHTVYNQGVTCIDDTVDAFFLDGTVPA
                     SDPMC"
     gene            complement(4305..4676)
                     /locus_tag="PLW15_07155"
     CDS             complement(4305..4676)
                     /locus_tag="PLW15_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT34206.1"
                     /translation="MLQDLYTVLPAQDLERARGFYHDKLGMEPEGTLDGMLMYRVGPA
                     GRFALYETSNAGTAQNTQMCLISDDLAADMARLRDAGLAFEEYDFPGMKTVDGVLDSG
                     ESRTAWFRDSEGNFVCLSQLA"
     gene            4905..5399
                     /locus_tag="PLW15_07160"
     CDS             4905..5399
                     /locus_tag="PLW15_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34207.1"
                     /translation="MGFRQWMHDHFVTPDAVYGLILYSALIGGVSDSDSNSVEVLIIS
                     AVSLLIFWGAHVFASTIATHGVKDGREITLGQATLRAFSHSSGMLYAAILPSLPLIVG
                     AFGLVSTDDAVSYSLLLAMIVLGVLGYQAFAQRGAKMGRRIWGGIGTAFFGFLMIVLN
                     VAVH"
     gene            5427..>6429
                     /locus_tag="PLW15_07165"
     CDS             5427..>6429
                     /locus_tag="PLW15_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HOT34208.1"
                     /translation="MNRRLTALFAAFEALLVVAIGVGIPLAPLTVLWGAQFGFGVDWG
                     AFWRASVDIWLIGHGVDVTVTLDPTTAAALGYPGAGDPFTITIAALGLGMLTLMLAVR
                     AGRRVAETRYRALGQIVALATFALLSFGVTFSSLHPLARPSLVQGTLLPTLVFAIGFV
                     IGVRLTRRHEDDTSTGRIRDWIAGWSPRARAIVATGLRAGATAAAGVVLAASLATALA
                     IAISYAKIITLYESLHTEVVGGFTLTLGQLAFIPNVVIWTASWLVGPGFAIGTGSNVS
                     ALGTQLGPIPAIPLLGALPTGSYAFGFVGLLVPVIAGFLAGALVGPGVRRRLGAEASG
                     "
BASE COUNT         1031 a         2248 c         2101 g         1049 t
ORIGIN      
        1 accagtaggc gtcgaaggcc gcgcggtcgg cgggccacag cgcgcgcgga acgcccagcg
       61 cggtgcccac gacggcgtag tcggcgagca gcgactcggc gtcggaatcg tcgagtggtc
      121 cgtagacgag ttcgcgcacc cgcatcgccg tctcgtacag cgtggcggcg acccagagct
      181 gcagttcgac gtcgttcgcg ccgggcacgg gcttgtgcgc gcccccgact cgccgggcga
      241 ccgccttcgc ctcgtcgggc gtgccgtaca cggttacgta caggtaggtc agggtgccgc
      301 gcaaccggtc gagcggacgg ttcgcgaagt cgctgtgctc ggcaacgccg acgccgacgc
      361 ccgggttcgc gacctgcagc aggatggccc gcgcgccgcc ggcgagcagt atcgcgtcag
      421 cggagacatc ctggatgcga agcggcaccc gcccattctg cgccccctcg cgaaagatgc
      481 gtcgttctta agcccgcccg aagaccgctc gcgaacgggg cgtccagatg gagcgagtag
      541 agttctccgt ggtcaaagtg tcttgatgtc gagctattcg acgtcgaccg cggaccgctc
      601 atcaccaccc ttcaacgcga ttgcgagagc cacgtggatc tgtacgagta ccaggccagg
      661 gatctgttcg agtcctacgg agtgccggta ctggccggca tcatcgccga caccccggcc
      721 gaggcgaagg ccgcagccga gaagatcggc ggcaccgtcg tcgtcaaggc tcaggtcaag
      781 gccggcgggc gcggcaaggc cggcggcgtc aaggtcgtgc acaacccgga tgacgcggcc
      841 accgccgctg aggcgatcct cggcctcgtc atcaagggcc tgaccgtcaa gcgcgtgatg
      901 gtcgcccagg gtgcccgcat cgcccaggag ttctacttct cgatcctgct cgaccgggcc
      961 aaccgctcct acctctcgct gtgcagcgtc gagggcggca tggagatcga ggagctcgcc
     1021 gtcgagcgtc ccgacgcgct cgcgaaggtc gaggtgaacc cgatcctcgg catcgatctc
     1081 gagaaggcga aagaaatcgc gctcgccggt ggcttccccg ccgacctggt cgagaaggtc
     1141 gctcccgtaa tcgtgaagct ctacgaggtc tacacgggtg aggacgcgac actggtggag
     1201 gtcaacccgc tcgtgctcac cgaggacggc caggtgctcg ccctcgacgg caaggtcacg
     1261 ctcgatgaga acgccggctt ccgccacccg aaccacgagg ccctcgagga caaggatgct
     1321 gcagacccgc tcgaggcgaa ggcgaaggcc aacgacctca actacgtgaa gctcgacggc
     1381 gaggtcggca tcatcggcaa cggtgccggg ctcgtgatgt ccaccctcga cgtggtgagc
     1441 tacgcgggtg agcggcacgg cggcgtgaag ccggccaact tcctcgacat cgggggcggc
     1501 gcatccgccg aggtcatggc caacggcctc gacgtgattc tcggcgacgc tcaggtcaag
     1561 agcgtgttcg tcaacgtctt cggtggcatc accgcctgcg acgcggtcgc caacggaatc
     1621 gtgcaggcgc tcgagatcct gggcgactcg gccaccaagc ccctggtggt gcgtctcgat
     1681 ggcaacaagg tcgaggaggg ccgcgccatc ctgcgcgccg ccaacaaccc gcttgtcacc
     1741 ctcgcagaca ccatggatga cggcgccgac aaggccgccg agctagcttc ccgctgatcg
     1801 agtagcgcag cgtatcgaga tcaaggatta ggaactaaac atgtcgatct tcctcaacaa
     1861 gtccaacaag gtcatcgtgc agggcatcac cggcggcgag ggcaccaagc acaccgccct
     1921 catgctgaag gccggcacgc aggtggtcgg cggcgtgaac gcacgcaagg ccggtaccac
     1981 cgtcgagcac gagggcggcg tcatcctgcc cgtgttcggc accgtcaagg aggcgatgga
     2041 ggccactggc gccgacacgt cgatcgtctt cgttccgccg gccttcgcca aggacgccgt
     2101 cgtcgaggcg atcgaggccg agatcccgct cgtcgtcgtg atcaccgagg gcatcccggt
     2161 gcaggacacc gccgagttct gggcgctcgc caaggccatg ggcaccacgc agatcatcgg
     2221 ccccaactgc cccggtatca tcacgcccgg cgagtcgctc gtcggtatca ccccggcgac
     2281 gatcacgggc aagggcccca tcggcctcgt ctccaagtcg ggcaccctga cctaccagat
     2341 gatgtacgaa ctgcgggacc tcggcttctc gactgcaatc ggcatcggcg gcgacccgat
     2401 cgtgggcacc acccacatcg acgcgctgcg cgccttccag gatgaccccg agaccaaggc
     2461 gatcgtgatg atcggcgaga tcgggggcga cgccgaggag cgcgcggcag acttcatccg
     2521 cgagtacgtg accaagccgg tcgtcggcta cgttgccggc ttcacggcac ccgagggcaa
     2581 gaccatgggt cacgccggtg cgatcgtctc ggccggcgcc ggtacggcgc agggcaagaa
     2641 ggaggccctc gaggccgcgg gcgtcaaggt gggcaagacg cccagcgaga cggctgcgct
     2701 catgcgcgag gtgctcgccg ctgggtagtc tggcggcatg acatctcgtc gcctgccact
     2761 cgtcgcggcc atcgccgctc tcagcctcgc ggccctgtcg ggatgccagt tcctcccgag
     2821 catcgtcggc ccgaccgaga ccggcccgac cggtcccgcg ccgaccgatg tcgcggggtt
     2881 ctactcgcag accgtcacct ggacggactg cagcgaggat ctcgagtgcg cgacggtgca
     2941 cgcccccacc aactggaact cgccctccga cggcgccatt gagctcgcac tcgtcaagca
     3001 caaggcgacc ggcacgaaga agggttcgct cctgctgaac ccgggcggcc cgggcggctc
     3061 gggctgggac tacgtgtact acagcggggc gtcgtcggcg accgcggcgg tctccgaagc
     3121 ctacgacatc gtcggatggg accctcgcgg cgtcggcaag tcgactccgg tcacctgcta
     3181 caccgacccg aagcagaccg acgaggtgct ctacggaacc tttgacagcc cctacggcac
     3241 gcagggctgg atcgacgagc tgaccgtcga ggaggagaag ttcgcggcag cctgccagca
     3301 gaacactgga gagcttcttg ccaacctcga cacggcgagc aacgccaagg acatggacat
     3361 gatccgggcg ctcgtcggtg acgagaagct caactacctg ggctactcct acggcaccta
     3421 cttcggcgcc gtctacgccg agctgttccc cgacaaggtc ggccgcctgg tgctcgacgg
     3481 tgccctcgac ccgctgctca gcgacttcga gtcgctcaag gtgcagatgg ccggcttcga
     3541 cagcgcgttc cgggcgtaca tgcagttctg cctgcagggc agcgactgcc cgttctccgg
     3601 cgccgtcgac acggcgctcg gtcaggcccg cacggtgctc gagactgtgg atgcccaagg
     3661 cctggtcaac agcgacggcc gggttctcga ctccgcgacc ctcggcaccg ccatcgccct
     3721 gaacctctac tccgaggact actggccgga catgacgcag atgttcgccg agctgagcac
     3781 gggcgacccc accctcgcct tcgtgaacgc ggactactac aactcccgca acgccgacgg
     3841 cagctactcc agcaactcgt tcgaggtcta caccgcggcc acgtgcgtcg acggcgactt
     3901 cgccgacgac cctgccacca ccctcgatcg tctcgccgag atcgacgcgg ccgcgcccat
     3961 cctcggcaag ttcttcgcct acgacgactt cgccgtgctc gacaccgcgt gctcgaactg
     4021 gccggtgccc cgcgccgacc tgccgatgag cttcgaggcg aagggcgcgc ccccgatcct
     4081 cgtgatcggc accaccaacg acccggcgac cccgtacgcc tgggcgcagg ccctggcatc
     4141 ccagctgtcg agcggcgtca tgatcacgca caagggcgag ggccacaccg tctacaacca
     4201 gggcgtgacc tgcatcgacg acaccgtcga cgcgttcttc ctcgacggta cggtgccggc
     4261 ctccgacccg atgtgctgag ggaggcgcgc agctgctgag cccctcaggc cagttggctg
     4321 agacagacga agttgccttc gctgtcgcgg aaccatgccg ttcgactctc accgctgtcg
     4381 agcactccgt cgacggtctt catgccggga aagtcgtact cctcgaacgc cagcccggca
     4441 tcgcgcaacc tggccatgtc ggcggcaaga tcgtccgaga tcaggcacat ctgggtgttc
     4501 tgggcggtgc ccgcattgga tgtctcgtag agagcgaaac gacccgccgg gcctactcgg
     4561 tacatgagca tcccgtcgag tgtgccctcg ggctccatcc ctagcttgtc gtggtagaag
     4621 ccgcgcgctc tttcgaggtc ctgggcgggc agcacggtat acaggtcttg caacatcgtc
     4681 cgactccttg gggccgatgg tgtggtccga gtgtgcggga ggggaattcg gcgtcattga
     4741 atcgacgcgg attcctctcc acggtcgtcg gcggtgccga cgcgagggta agtggccccg
     4801 actatacgcc gctggcacgc acccggctag tggtcgcccg caggctcagc gcggccccgg
     4861 cgagctttcc cgatgcctcc cgacccgccc ggtacggtag ccccatgggt ttcaggcagt
     4921 ggatgcacga tcacttcgtg accccggatg ccgtgtacgg cctcatcctg tactccgcgc
     4981 tcatcggcgg ggtgtcggac tcggacagca actcggtcga ggttctgatc atctcggcgg
     5041 tgtcgctgct gatcttctgg ggcgctcacg tgttcgcgag cactatcgcc acccacggcg
     5101 tcaaggacgg cagggagatc actctcgggc aggcgacgct gcgcgcgttc agccactcct
     5161 cgggcatgct ctacgcggcc atcttgccgt cgctgcccct gatcgtcggc gcgttcggcc
     5221 ttgtgtccac ggatgacgcg gtgagctact cgctgctact cgccatgatc gtgctgggtg
     5281 ttctcggcta ccaggccttc gcccagcgcg gagcgaagat gggccggcgc atctggggtg
     5341 gcatcggcac ggcgttcttc ggcttcctca tgatcgtgct caatgtcgct gtgcactgaa
     5401 cgcccagtga tcgaccccgg tagccgatga atcgccgact caccgccctg ttcgccgcgt
     5461 tcgaggcgct gctcgtggtg gccatcggcg tcggtatccc actcgcaccg ctgacggtgc
     5521 tgtggggagc ccagttcggg ttcggtgtcg actggggtgc attctggcgg gcatccgtcg
     5581 acatctggct catcggtcac ggcgtcgacg tgaccgtcac gctcgacccg accaccgcgg
     5641 cggcgctcgg ctaccccggc gccggtgacc cgttcacgat cacgatcgcc gctctggggc
     5701 tcgggatgct caccctcatg ctcgccgtgc gggccggtcg acgggtcgcc gagacccgct
     5761 accgggccct cggccagatc gtggccctgg ccacattcgc gctgctgtcg ttcggcgtga
     5821 cgttctcgag tctgcacccg ctcgcgcgcc cctcgctggt gcagggcacc ctgctgccga
     5881 cgctggtgtt cgcgatcggc ttcgtgatcg gtgttcggct gacccgccgg cacgaggacg
     5941 acacctcgac cggtcggatc cgggactgga tcgcgggctg gtcaccgcgt gcccgggcca
     6001 tcgtcgccac tggtcttcgc gcgggcgcaa ccgcggcggc cggggtcgtg cttgccgcgt
     6061 cgctcgccac ggcccttgcc atagcgatct cctacgcgaa gatcatcacg ctctacgaga
     6121 gtctgcacac cgaggtcgtc ggcggcttta cgttgaccct cggccagctc gcgttcatcc
     6181 ccaacgtcgt catctggacg gcgagctggc tggtcggccc cggtttcgcg atcggcaccg
     6241 ggtcgaatgt cagcgcgctc ggcacccagc tcggtcccat cccggcgatc ccgcttctcg
     6301 gcgccctacc gaccggcagc tacgccttcg gattcgtggg gctcctcgtg cccgtgatcg
     6361 cgggcttcct cgcgggggcg ctcgtcggtc ccggcgtgcg tcgacggctg ggcgccgaag
     6421 ccagcggca
//
LOCUS       DAOSOU010000111         6391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12254_length_6391_cov_7.312342, whole genome shotgun sequence.
ACCESSION   DAOSOU010000111 DAOSOU010000000
VERSION     DAOSOU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12254_length_6391_cov_7.312342"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLW15_07170"
     CDS             complement(<1..336)
                     /locus_tag="PLW15_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT34209.1"
                     /translation="MTDALSRHTDYGQTPLNESDVLSDPIAQARLWLDQAAESDVYEP
                     NAMVLSTIDPDGLPSSRTVLLRALDERGLEFFTSYTSAKGRALLAHPEVSIVFPWYLI
                     HRQIIVRGVA"
     gene            complement(333..1589)
                     /locus_tag="PLW15_07175"
     CDS             complement(333..1589)
                     /locus_tag="PLW15_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34210.1"
                     /translation="MDVLAGLATGIVTLVVVGGGLVVVGFVVLFRRRGDRGIRSSNPS
                     GIDALNRKAGTLLVKLDDAVRDADDEIGYALAQFGPEKSRPYGDAVANARAKVTEAFR
                     LKQALDDAVPDSDRQRREWTLQIIALCEQAEASLADQATRFTELRSREVNAAGTLDDL
                     RRRIETTTGRLATSRALLADLAKTYAPSTLAAVTANPAEAEKALKAASTGADAAAPAI
                     SQTGVNDVSTQLAEASQAAHRADQLLDAVDRTARDLAAASTALADLRTRSTADVAEAK
                     ALKAPDAETGEAILDAVGVVEKALAETTKDPVADLDRIGDAVAQLDLALASARNQAQR
                     LEHARTAYLGTVVSATNQIATAREFIAGHGGSVEARTRLAEAERQFTIAQAAADPVEA
                     LDTIRRSVTLAQDADALARYDAMGAR"
     gene            1728..>2161
                     /locus_tag="PLW15_07180"
     CDS             1728..>2161
                     /locus_tag="PLW15_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34211.1"
                     /translation="MPETGPLDTPMQVTAPHTLSFGEAGLVPGNAGEPAWAAWRAELA
                     ALGGRSPLLHFVDSPRTRIELSTTHPGGLAQFITGKSTLLSSLIRDEIALRSAKIAAS
                     AVEAKGLELSTARGIDSVHLGIGIADWTFGGEEFSAPVLLRPL"
     assembly_gap    2162..2261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2262..5156
                     /locus_tag="PLW15_07185"
     CDS             <2262..5156
                     /locus_tag="PLW15_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34212.1"
                     /translation="LDADAEQENVIAQIAAGNSLVVKTLPGTGGTQTIVNAIGSLVTQ
                     NKRVLVVSARRASLNSIAQRLTDVGLPGLAVAPRSLRRDIIRSIGRSEKARQPNVGEV
                     DDALLRLRKVLLDYRGALTRVDPQLGVSVLDCVTELSRLALLPSPPSTTARLSRSAVE
                     RLTADRTRATEIMVAAAQLGEFKYGPGDSPWYGAQFANGDAALRAHEIAKRLAADELP
                     RLLPMAQQLVAGTHMRPFTTIAELGIYLRLLVDLRDTLDKFLPMVFDRSISELIAATA
                     PRKDFPDMSNGNRRRLKKLALEYVRPGVRVGDLNAALIRIQHQRVLWHRFVAEGAIPE
                     VPVGIGDVQVAFQQVSQDLAALDEPLGLTGRDSQLDSVPIESLVAKIEALAAESDVLH
                     NLQDRTALMTALRDLDLDPLIDDLARRHVPEEQVAAELELSWWKSALESLLEADRALL
                     NANTSVLDRLEADFRLVDEAHAAGSAQLLAWQLAENWKIGLVDWPDEANLLKRLLSRD
                     SVTAFDLQTTAPHLSRTVAPVWLASPYEIDRIIDTMPFDTVILVDAGATTVAESAGAI
                     RRAKQVVAFGDPVTQTPAAFAIAVSTGSTTGVSTGGVSTSSTTAADDIHSQSALARLG
                     ALLPTLTLTRSYRPGGEDLAELVNRRFYGGRIQSLPWAGSFLGHGSLALDYVADGHGM
                     PDPDSGAVESPDAEVDRVVDLVLDHASKRPHESLMVITASEKHAVRVMQAVLGAVGSR
                     PELVDFVIGDRPEPFAVTTIDQAVAQSRDRVIFSIGYGRTPHGRVLSDFGALGEPGGE
                     RLLAVAMTRARRSMVIVTSFEPEDLDDGRMKHGAVALAEILGEVGARQIEQPVPDDSE
                     PMLVDLAQRLEARGLNVALGHRGKLGLVASRGGMCLTIETDAVLNRGTLRESLRLRPE
                     LLRRLGWHYLRVHAFDLFSDPDAVAARVATMLGADAAPITEPITIVASNA"
     gene            complement(5559..5975)
                     /gene="mscL"
                     /locus_tag="PLW15_07190"
     CDS             complement(5559..5975)
                     /gene="mscL"
                     /locus_tag="PLW15_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOT34213.1"
                     /translation="MLKGFKEFILRGNVIDLAVAVVIGAAFTGIVTAIVGSVLNPALG
                     ALFNAEDLAKALPVNIPTVSGGTATMYFGAVIAAIIQFLLVAVVVYFGLIVPINYVKR
                     VSFRRRAEPDATAEPAPPTELELLAEIRDLLASRKD"
     gene            complement(5985..6323)
                     /locus_tag="PLW15_07195"
     CDS             complement(5985..6323)
                     /locus_tag="PLW15_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT34214.1"
                     /translation="MPTYSYRCTECSNAFDIHQSFTDDSLTVCPTCGGVLRKVFSPVG
                     VTFSGSGFYRTDSRAPAKSEGSGSSSSASSASASDSGSSSTPAPAAAKTEKKSSTPAS
                     SGSASGGTSS"
BASE COUNT          948 a         2172 c         2159 g         1012 t
ORIGIN      
        1 cgcaaccccg cgcacgatga tctggcggtg gatgagatac caggggaaca cgatcgagac
       61 ctcgggatgc gccagcagcg cccgtccctt ggccgacgtg tagctcgtga agaactccag
      121 gccgcgctca tcgagcgccc gcagcagcac agtgcggcta gagggaagtc cgtcgggatc
      181 gatcgtcgac agcaccatcg cgttcggctc gtagacgtcc gactcggcgg cctggtcgag
      241 ccacagccgg gcctgcgcga tcggatcgct cagaacatcg gactcgttca gcggcgtctg
      301 cccgtagtcg gtgtgcctgc tcagtgcgtc ggtcatcgtg ctcccatggc gtcgtatcgt
      361 gcgagtgcat cggcgtcctg cgccaaggtc accgatctgc ggatcgtgtc gagggcctcg
      421 accggatcgg cggcggcctg tgcgatcgtg aactggcgct ccgcctccgc aagccgggtg
      481 cgtgcctcga ccgatccgcc gtggcccgca atgaattccc gcgcggtggc gatctggttc
      541 gtcgcggaga cgacggtgcc gaggtaggcg gtgcgggcgt gttccaggcg ttgcgcctgg
      601 ttgcgcgcgc tcgcgagggc gaggtcgagt tgtgcgacgg catcgccgat gcgatcgagg
      661 tcggcaaccg ggtccttcgt cgtctcggcg agcgccttct cgacgacgcc gacagcatcg
      721 aggatcgcct cgccggtctc ggcgtcgggt gccttcaggg ccttggcctc cgcgacgtca
      781 gccgtgctgc gggtgcgcag atcggcgagc gccgtgctcg cggcagcaag atcgcgagcg
      841 gtgcggtcca cggcgtcgag aagctggtcg gcccggtggg ccgcctggga tgcctcggcc
      901 agttgggttg agacgtcatt gaccccggtc tgcgagatgg cgggggccgc ggcatccgcg
      961 ccggtcgagg cggccttgag cgccttctcg gcctcggcgg gattcgccgt gactgccgcc
     1021 agggtgctgg gggcgtaggt cttcgcgaga tcggcgagca gggcccggga cgtggcgagg
     1081 cgaccggtcg tcgtctcgat gcggcggcgc aggtcgtcga gcgtgcccgc ggcgttgacc
     1141 tcccgcgagc gcagttcggt gaagcgggtg gcctggtcgg ccagggatgc ctcggcctgc
     1201 tcgcacagcg cgatgatctg cagggtccac tcccgccgct gccggtcgga atcgggcacg
     1261 gcgtcgtcga gcgcctgctt gagccggaag gcctcggtca ccttggcgcg cgcgttcgcg
     1321 acggcatcgc cataggggcg ggacttctcg gggccgaact gcgccagcgc atagccgatc
     1381 tcgtcgtcgg catcgcgcac ggcgtcgtcg agcttcacga gcagcgttcc cgccttgcgg
     1441 ttcagtgcgt cgatgccgct ggggttcgag ctgcggatgc cgcgatctcc ccgacgccgg
     1501 aacagcacga cgaaaccgac cacaaccagc ccgccgccga ccaccacgag tgtcacgatg
     1561 cccgtagcga gccctgcgag cacgtccatg ggcctcattc tacgactggc ccggttcgcg
     1621 aaactcggcc cgttctcccg agtttccgca ggtcacgcac ttaaggttgc ggtgtgtggc
     1681 gtgccgatcg aacctcccgc gacttcagtg tcgacccgga gctcgtcatg cccgaaaccg
     1741 gcccgctgga caccccgatg caggtgacgg cgccccacac gctcagcttc ggcgaggcgg
     1801 ggctggttcc cggcaacgcg ggtgagccgg cctgggcggc ctggcgcgcg gaactcgcgg
     1861 ccctcggcgg tcgctcgcca ctgctgcact tcgtcgactc cccgcgtacg cgcatcgaac
     1921 tgtccacgac gcatcccggc ggtctcgccc agttcatcac cggcaagtcg acactgctgt
     1981 cgagtctgat ccgcgacgag atcgcgctgc gcagtgcaaa gatcgcagca tcggcggtcg
     2041 aggccaaggg tctcgagctc tcgacggcgc gcggcatcga cagcgtgcac ctcggcatcg
     2101 gtatcgccga ctggactttc gggggcgagg agttcagcgc ccccgtgctg ctgcgtcccc
     2161 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctcgacgct gacgccgagc
     2281 aggagaacgt gatcgcccag atcgcggcgg gcaactcgct ggtcgtcaag accctgccgg
     2341 gaaccggcgg cacccagacg atcgtcaacg ccatcggaag tctcgtcacg cagaacaagc
     2401 gcgttctcgt ggtcagtgcc cggcgggcat ccctgaactc gatcgcgcag cggctgaccg
     2461 acgtgggcct tccgggactc gcggtcgcgc cgcggtcgct gcgtcgcgac atcatccgct
     2521 cgatcggtcg cagcgagaag gctcgccagc ccaatgtcgg cgaggtggat gacgccctcc
     2581 tgcgcctgcg caaggtgctg ctggactacc gcggtgctct cacccgcgtc gacccgcagc
     2641 tcggggtctc ggtgctggac tgtgtcaccg aactgagcag gcttgcgttg ctgcccagcc
     2701 cgccgtcgac gacggcccga ctgtcgaggt ccgccgtcga gcggctcacc gccgaccgca
     2761 cgcgcgcgac cgagatcatg gtcgctgccg cccagcttgg tgagttcaag tacggccccg
     2821 gcgactcgcc ctggtacggg gcccagttcg ccaacgggga tgccgcgttg cgcgcgcacg
     2881 agatcgccaa gcgcctggcg gccgacgaac tgccgcgcct gctgcccatg gcccaacaac
     2941 tcgttgcggg cacccacatg cgtccgttca cgacgatcgc ggagctggga atctacctgc
     3001 gccttctcgt cgacctgcgc gacactctcg acaagttcct gccgatggtc ttcgaccgtt
     3061 cgatcagcga gctgatcgcg gcaaccgcgc cgcgcaagga tttccccgac atgtcgaacg
     3121 gcaaccggcg tcggctcaag aagctggccc tggagtacgt gcgtccgggt gtgcgggtgg
     3181 gcgacctcaa tgccgccctc atccgcatcc agcaccagcg cgtgctctgg catcgattcg
     3241 tggccgaggg tgcgattccc gaggtgcccg ttggcatcgg cgacgtgcag gtcgcgttcc
     3301 agcaggtctc ccaggatctt gccgccctcg acgaaccgct cgggctcacc ggacgggact
     3361 ctcaactcga ctccgtgccg atcgagtcgc tggtcgccaa gatcgaggct cttgccgccg
     3421 aatcggatgt gctgcacaac ctgcaggatc gcacggccct gatgacggct ctgcgtgacc
     3481 tggatctcga cccgctcatc gacgacctcg cccgccgcca tgtgccggag gagcaggttg
     3541 ccgccgagct cgagctgtcg tggtggaagt cggctctgga gtcgctcctc gaagccgacc
     3601 gcgctctgct caacgcgaac acctcggtgc tcgatcgtct ggaggccgac ttccgtctcg
     3661 tcgacgaggc gcacgccgcg ggcagcgccc agcttctggc atggcagctc gccgagaact
     3721 ggaagatcgg tctcgtcgac tggcccgacg aggcgaacct cctcaagcgc ctgctctccc
     3781 gcgactccgt caccgcgttc gaccttcaga ccacggcgcc gcacctgtcg cgcaccgtcg
     3841 cgccggtctg gctcgcatct ccctacgaga tcgaccgcat catcgacacg atgccgttcg
     3901 acacggtgat tctcgtcgac gccggcgcaa ccaccgtcgc cgagagtgct ggcgcgattc
     3961 gccgagccaa gcaggtcgtc gcgttcggtg acccggtcac gcagacacct gcagcgtttg
     4021 cgattgcagt ctcgacaggc tcgaccaccg gggtctcgac gggcggggtc tcgacaagct
     4081 cgaccaccgc ggccgacgac atccattcgc agtcggcact tgcccggctc ggagcactcc
     4141 tgcccacgct gaccctcacg cgcagctacc ggcccggcgg cgaggacctc gcagagctcg
     4201 tcaaccgccg gttctatggt gggcgcatcc agtcgctgcc ctgggccggc agcttcctcg
     4261 gtcacggcag cctcgccctc gactacgtcg cggacgggca cggcatgccc gaccccgatt
     4321 ccggcgcggt cgagagcccg gatgccgagg tcgaccgcgt cgtcgatctc gtactggacc
     4381 acgcctcgaa gcgcccccac gagtcgctca tggtcatcac cgcgagcgag aagcacgcgg
     4441 tacgggtgat gcaggcggtg ctcggtgccg tcggatcgcg gcccgaactc gtcgacttcg
     4501 tcatcggtga ccggcccgag cccttcgccg tgacgaccat cgatcaggcc gtcgcgcaga
     4561 gccgggaccg ggtgatcttc tcgatcggct acgggcgcac cccacacggc cgggtgctgt
     4621 ccgacttcgg cgccctcggt gagcccgggg gcgagcggct gctcgcggtc gccatgacgc
     4681 gcgctcggcg ttcgatggtg atcgtgacat ccttcgagcc ggaagacctc gacgacggtc
     4741 gcatgaagca cggcgcggtc gcgctcgctg agattctcgg ggaggtcggt gctcgccaga
     4801 tcgagcagcc ggtgcccgac gacagcgagc ccatgctggt cgatttggcc cagcgcctcg
     4861 aggcccgagg acttaacgtc gcgctcggtc accgcggaaa gctgggtctc gtcgcgtccc
     4921 gcggcggcat gtgcctgacc atcgaaacgg atgcggtgct caaccgcggc acgctgcgcg
     4981 aatcgctgcg cctgcgtccc gagctgttgc gccgtctcgg atggcactac ctgcgggtgc
     5041 acgcattcga tctgttcagc gatcccgatg ccgtcgccgc cagggtggcg acgatgctgg
     5101 gggcggacgc cgcgccgatc accgagccga tcaccatcgt cgcgtcgaat gcctgaccac
     5161 cgggccgagg atcacgacca catcgcgcca tcgccgcggc gcacgcgggg cgcacgccgc
     5221 gtgaccaccc cggcgcccga gggatcggat ccctctccgg caccagagcc ctcccggcac
     5281 cacgtcgacg agaacgacga gcagctcaag cgcgacaagc cgccgcactg gggctagcgg
     5341 gagtttctga catcccttag gccctgccgt ctcctcgcag cccgtctcgg cgcgcatctg
     5401 gaaggccggt cgtggcgact ccccgaactc aggacattag gcagggtcgc gaacaaatgt
     5461 cctgagttcg gggagcgtgc cggctaacag cgcccggtga gccgtcaggc cggtgagccg
     5521 tcgggcgggt ccgccgccgc actgggggta gcgggcgatc agtccttgcg gctcgcgagc
     5581 aggtcgcgga tctcggcgag cagctccagc tcggtgggcg gtgcgggctc ggccgtggca
     5641 tccggttcgg cgcgacgacg gaacgacacg cgcttgacgt agttgatcgg cacgatgagg
     5701 ccgaagtaga cgaccaccgc aaccagcagg aactggatga tcgccgcgat caccgcaccg
     5761 aagtacatgg tggcagtgcc gcccgacacg gtcgggatgt tcacgggcag cgccttggcg
     5821 aggtcctcgg cgttgaacag cgcgccgagc gcggggttca gaacgctccc gacgatcgcg
     5881 gtgacgatgc ccgtgaatgc ggcaccgatc actacggcga cggcgagatc gatgacattg
     5941 ccgcggagga tgaactcctt gaagcccttt agcacgacgt gctcctaact gctggtgccc
     6001 cctgaggcgg atcccgagga ggcgggggtg gacgacttct tctcggtctt ggccgcggcc
     6061 ggcgcgggag tcgagctcga gccggagtcg ctcgctgagg cactcgaggc gctcgacgag
     6121 ctgcccgacc cctcgctctt cgcgggtgcg cgggagtcgg tgcggtagaa gccagacccg
     6181 gagaacgtca cgcccaccgg cgagaagacc ttgcgcagca cgccgccgca ggtcgggcac
     6241 acggtgaggg agtcgtcggt gaacgactgg tggatgtcga aggcgttgct gcattcggtg
     6301 cagcggtacg agtaggtggg cacggtgttc cttggtgtgg agagctagaa ggcgacgatg
     6361 ccagaagggg tgacgacccc gtcgacgcgc t
//
LOCUS       DAOSOU010000112         6370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12311_length_6370_cov_7.087569, whole genome shotgun sequence.
ACCESSION   DAOSOU010000112 DAOSOU010000000
VERSION     DAOSOU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12311_length_6370_cov_7.087569"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLW15_07200"
     CDS             <1..507
                     /locus_tag="PLW15_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOT34215.1"
                     /translation="SRYCESDSLTPTARSEFAGLSGHDLLSAVTLWVWERLRYVPGSS
                     LPTDGAEQTLLARRGVCRDYAHLVIALLRALDVPARMVAVYAPGLSPMDFHAVAEAWV
                     DGAWWTVDATRLAPRQSMLRISTGRDAADIAFLTNHWADLSLTRMEVLAIVDELPSDD
                     GTQLVQLG"
     gene            complement(508..1089)
                     /locus_tag="PLW15_07205"
     CDS             complement(508..1089)
                     /locus_tag="PLW15_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HOT34216.1"
                     /translation="MRTRRTLVAILPLSLLLLTACVPTGSATPSGSATPSASTSASAT
                     PSATPTASAAPSTPVTIGCDSLISLQEMYDFNPNFSAKADYSPAAGSLGAEALERKGI
                     ACAWVNQTSGELIEISVANPGDTVLTAKKNELVTTSNPVPTYEVEGYFIVDNGVGTAQ
                     AFDDPYWIVATSSLFFEPGDVAPLMAAAIANLG"
     gene            1222..2880
                     /gene="argS"
                     /locus_tag="PLW15_07210"
     CDS             1222..2880
                     /gene="argS"
                     /locus_tag="PLW15_07210"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT34217.1"
                     /translation="MNPADLSAALLTVVTGIVTRRGGTAELTVDDVALERPRNRDHGD
                     WASNIAMKLAKSLGANPRELAQEIADAAAELEGVASVDVAGPGFINITLDAAAAGALA
                     KTIVEHADAYGLGDLYRGVTINLEFVSANPTGPIHIGGVRWAAVGDSLARIFQAQGGI
                     VTREYYFNDHGAQIDRFARSLLASWLGEPAPEDGYGGDYIADIAARVLTLYPGDLFHI
                     PRDEQQEVFRAVGVELMFGDIKASLHEFGVKFDVYFHENELHESGAVNHAIDRLRELG
                     AIYEADGATWFRSTDYGDDKDRVVIKSDGEFGYMSGDLAYYLNKRERGFERNLIMLGA
                     DHHGYVKRLMAMTAAFGDTPYVNLEILIGQLVNLVRNGEPVRMSKRAGTVVTLEDLVE
                     AVGVDAGRYALVRSSIDSQVDIDLDLWGKKTNDNPVFYVQYAHARTHSVARNASGSGV
                     DRSAFDASLLDHETESILLGILAEYPRLLRQAAELREPHRVARYAEELAGAYHRWYDS
                     CRVTPVGDEPVGDVHRTRLWLNDAVGQVLRNGLGLLGVSAPERM"
     gene            2883..3761
                     /locus_tag="PLW15_07215"
     CDS             2883..3761
                     /locus_tag="PLW15_07215"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HOT34218.1"
                     /translation="MAEPIEPVVEPVVEPVETKTAVLPATPAATPPAQPRKRRRWIGW
                     VIALVVLVVLLVVGFFVADAYARDYAKGYVRDQIIQVLKLDPATKVDVDLGGGSVILQ
                     ALRGSIDEVTVDVAELSFGDITGSAVITAAGVPLDGAQPVDKLGIVATVSETNVRKLA
                     SFMSGIELKTIELGDGVITASTEFNVLGFFVIPVSVDLVPSAVDGGISFDPKTITLAG
                     EDISVADLRASPEFAGIAGQFLQSQDFCVASSLPKALTITDVDVVGSNLVIAINGDGT
                     ALGGPGLSELGVCKAK"
     gene            complement(3762..5132)
                     /locus_tag="PLW15_07220"
     CDS             complement(3762..5132)
                     /locus_tag="PLW15_07220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT34219.1"
                     /translation="MSDTAATLQFLGAADTVTGSRYLVTAQQRRILVDCGLFQGYKVL
                     RERNRAQFPIDPSSIDSVVVSHAHLDHSGYLPALVRDGFAGPIHVTHGTAELLGLLLL
                     DSAHLLEEEAKFAARHGFSRHEHPQALYTVEDAERALERIVPVDFDHTVELGPELTAT
                     LVPAGHILGAAQVRIAHAGRTVHFSGDLGRTDDPLMRAPRALEPTDIVVCESTYGDRA
                     HPAADPADELASVISRVASRGGVVIIPAFAVGRTQELLLHLARLRREGRIPEVPVYLN
                     SPMAKDATSLYRSHREENRISDGDFEDMYNLATIVTSVDDSKLLNLRGGPMIIISASG
                     MLTGGRVLHHVVAYGDDPANAIVLSGFQAGGTRGAALAAGADHLRIFGKDVPIRAEVV
                     QLQTMSAHADADGVLAWLGAVPTPPEMIYVTHGEPASADTLRARIRHELGWNARVPEQ
                     SETVQI"
     gene            5291..>6370
                     /locus_tag="PLW15_07225"
     CDS             5291..>6370
                     /locus_tag="PLW15_07225"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT34220.1"
                     /translation="MSSNPLAPAWLVEPADANALPAAVWSHNARRSAGGELEIAGVSA
                     SALVAEFGTPVYVVDEADARSRAGGIRESFDREFARVGSSAKVYYAGKAFLCTEVARW
                     VTEAGLNIDVASGGEMAVALAAGVDPARLGLHGNNKSLSEIDRAVGAGVGAIVIDSVV
                     EIERVATAASRHGRTQPVRLRINSGVHASTHEYLATAREDQKFGIPLADAAAAVALIR
                     AQPSLTFLGLHSHIGSQIFESDGFAEAAHRLFALHAELLKGGPVPELNLGGGFGIAYT
                     SVDDPLDIDEIARRFADIVSQGAAELGIPVPVVAIEPGRAVIGPSTTTLYTVGTIKDV
                     LVSVVEPVETCSEAHAEISTSSIAGT"
BASE COUNT          996 a         2185 c         2140 g         1049 t
ORIGIN      
        1 agccgctact gcgagtccga ctcgctcact cccacggccc gcagcgagtt cgcgggcctc
       61 agcggccacg acctgctctc cgcggtcacg ctctgggtct gggagcggct gcgctacgtg
      121 ccaggctcga gcctgcccac cgatggcgcc gagcagaccc tgctcgcccg ccgaggcgtc
      181 tgccgcgact acgcgcacct cgtcatcgcc ctgctccggg ctctcgacgt tcccgcccgc
      241 atggtcgccg tctacgcgcc gggcctgtcg ccgatggact tccacgcggt ggccgaggcc
      301 tgggtcgatg gcgcctggtg gaccgtggat gccacgcgcc tggcaccccg ccagagcatg
      361 ctgcgcatct caacgggccg tgacgcggcg gacatcgcct tcctcacgaa ccactgggcc
      421 gacctgtccc tgactcggat ggaggtgctc gcgatcgtcg acgagctgcc gtccgacgac
      481 ggcactcagc tcgtgcagct cggctagcta gccgaggttg gcgatggccg cggccatgag
      541 cggcgcgaca tccccgggct cgaagaacag cgacgaggtc gcgacgatcc agtaggggtc
      601 atcgaacgcc tgggcggtgc ctaccccgtt gtccacgatg aagtagccct cgacctcgta
      661 ggtgggcacg gggttgctgg tggtcaccag ctcgttcttc ttcgccgtca acacggtgtc
      721 gccggggttg gccacggaga tctcgatcag ctcgccactg gtctggttga cccaggcgca
      781 ggcgatgccc ttgcgctcga gggcttcggc gccgagcgac cccgcggcgg gggagtagtc
      841 ggccttcgcg ctgaagttgg ggttgaagtc gtacatctcc tgcagtgaga tgagcgagtc
      901 gcatccgatg gtcacgggcg tcgagggagc cgcgctcgcc gtgggagtcg cactcggcgt
      961 cgcgctggca cttgtcgacg ccgacggcgt ggcactcccg ctgggcgtgg ccgacccggt
     1021 cggcacgcac gccgtgagca gcagcaggct gagggggagg atggccacaa gggttcgacg
     1081 agtgcgcatg gtcctatcct caccacggtt gcgtgtgagt gtgctgccaa agcgcgcgcg
     1141 tcggcatttc gggcatccaa ctcgatcacc ccactggccg gtcggattcg gccggaggcg
     1201 ccccggctaa gcttgtgcct cgtgaatccc gccgatctct ccgccgccct gctcaccgtc
     1261 gtcacgggca tcgtgacgcg acgcggcggc acggcggagc tcacggtcga cgacgtcgcc
     1321 ctcgagcgcc cgcgcaatcg cgaccacggg gactgggcct ccaacatcgc catgaagctc
     1381 gccaagtcgc tcggtgcgaa cccccgggag ctcgcgcagg agatcgccga cgcggccgcc
     1441 gagcttgagg gcgtggcatc cgtcgatgtg gcgggccccg gcttcatcaa catcactctg
     1501 gatgccgccg cagccggcgc cctggccaag accatcgtcg agcacgccga cgcctacggg
     1561 ctcggcgatc tctaccgcgg cgtcaccatc aacctcgagt tcgtgtcggc gaacccgacc
     1621 gggccgatcc acatcggtgg cgttcgctgg gccgcggtgg gcgacagcct ggcgcgcatc
     1681 tttcaggcgc agggcggcat cgtcacgcgc gagtactact tcaatgacca cggcgcgcag
     1741 atcgaccggt tcgcccgcag cctgctcgcg agttggctcg gcgagcccgc cccggaggac
     1801 ggctacggcg gcgactacat cgccgacatc gcggcgcgcg tgctcacgct ctacccgggc
     1861 gacctgttcc acattccgcg cgacgagcag caggaggtgt tccgggcggt cggcgtcgag
     1921 ctgatgttcg gcgacatcaa ggcgagcctg cacgagttcg gcgtgaagtt cgacgtctac
     1981 ttccacgaga acgagctgca cgagtcgggc gccgtgaacc acgccatcga ccggctgcgc
     2041 gagctcggcg cgatctacga ggccgacggc gcaacgtggt tccggtcgac cgactacggc
     2101 gacgacaagg accgcgtcgt catcaagagc gacggtgagt tcggctacat gtccggcgac
     2161 ctcgcctact acctcaacaa gcgcgagcgc ggcttcgagc gcaacctgat catgctcggc
     2221 gccgaccacc acggctacgt caagcgcctc atggcgatga cggccgcgtt cggcgacacc
     2281 ccgtacgtga acctcgagat cctcatcggc cagctcgtga acctggtgcg caatggcgaa
     2341 cccgtgcgca tgtccaagcg cgcgggcacc gtcgtcactc tcgaggacct cgtcgaggcc
     2401 gtcggcgttg acgccggtcg ctacgcgctt gtgcgcagct cgatcgactc gcaggtcgac
     2461 atcgacctcg acctgtgggg caagaagacc aacgacaacc ccgtgttcta cgtgcagtac
     2521 gcgcacgccc gcacgcacag cgtcgcgcgc aacgcttccg gctccggggt ggaccgcagt
     2581 gcgttcgacg cgagcctgct cgaccacgag accgagagca tcctgctggg catcctcgcc
     2641 gagtacccgc ggctgctgcg ccaggccgcc gagctgcgcg agccgcaccg cgtcgcccgc
     2701 tacgccgagg aactcgccgg cgcctaccac cgctggtacg acagctgtcg ggtcaccccg
     2761 gtcggtgacg agccggtcgg cgacgtgcac cgcacccgcc tctggctgaa cgacgccgtc
     2821 ggacaggtgc tgcgcaacgg tctcggactg ctgggcgtca gcgccccgga gaggatgtag
     2881 caatggccga gcccatcgaa ccggtggtcg agcccgtggt cgagcctgtc gagaccaaga
     2941 ccgcggtgct cccagctaca cctgccgcaa cccctcccgc acagccccgc aagcgccgcc
     3001 gctggatcgg ctgggtcatc gccctcgtcg tacttgtcgt gctgctcgtc gtgggcttct
     3061 tcgtggccga cgcctacgcg agggactacg ccaagggcta cgtgcgcgac cagatcatcc
     3121 aggtgctgaa actcgacccg gccacgaagg tcgatgtcga cctcggtggc ggctccgtca
     3181 tcctccaggc cctgcgcggt tccatcgacg aggtcacggt ggatgtcgcc gagctctcct
     3241 tcggcgacat cacgggttct gccgtgatca ccgccgcagg ggtgcccctc gatggtgcgc
     3301 agccggtcga caagctcggg atcgtcgcca cagtctccga gaccaacgtg cgcaagctcg
     3361 cgagcttcat gagcggcatc gaactcaaga ccatcgaact gggcgacggg gtcatcaccg
     3421 cgagcaccga gttcaacgtt ctcggcttct tcgtgattcc ggtgtcggtc gacctcgtgc
     3481 cctcggccgt cgatggcggc atcagcttcg acccgaagac gatcacgctc gcgggcgagg
     3541 acatctcggt ggccgacctg cgcgcgagtc cggagttcgc ggggattgcg gggcagttcc
     3601 ttcagtctca ggacttctgc gtcgcatcca gcctgccgaa ggctctgacg atcaccgatg
     3661 tcgacgtggt cggctccaac ctggtcatcg cgatcaacgg cgacggcacc gcgctgggcg
     3721 gtccggggct gtccgaactc ggggtctgca aggcgaagta gtcagatctg caccgtctcc
     3781 gactgctcgg gcacgcgcgc gttccaaccc agttcgtgac gaatccgtgc acgcagggtg
     3841 tcggcagacg caggctcacc gtgggtcacg tagatcatct cggggggtgt cgggaccgct
     3901 cccaaccagg cgagtacgcc gtccgcgtcg gcatgcgccg acatcgtctg cagttgcacg
     3961 acctcggccc tgatcgggac atccttgccg aagatgcgga ggtggtcggc accggcggcc
     4021 agcgcggctc ctcgagtccc acccgcctgg aacccgctca gcacgatggc gttcgcggga
     4081 tcgtcaccgt acgcgaccac gtggtggagc actcggccgc cggtgagcat cccgctcgcg
     4141 gagatgatga tcatcggccc accgcggagg ttgaggagct ttgagtcatc cacgctggtc
     4201 acgatcgtcg cgaggttgta catgtcctcg aagtcgccgt cggagatgcg gttctcctcc
     4261 cggtgcgatc ggtagaggct ggtcgcgtcc ttcgccatgg ggctgttgag gtacaccgga
     4321 acctcaggaa ttcgaccctc cctgcgcagc cgggcgaggt ggagcagcaa ttcctgggtt
     4381 ctaccgaccg cgaaggcggg gatgatcacg acgccgccgc gggaggcgac acgggagatc
     4441 acagaggcca actcatccgc cggatctgcc gccgggtgcg ctcggtcgcc gtaggtcgat
     4501 tcgcacacca cgatgtcggt cggctcgaga gcgcgtggcg cgcgcatgag cggatcgtcc
     4561 gtgcgtccga gatctccgga gaagtgaacg gttctgcccg cgtgcgctat ccgcacctgt
     4621 gccgcgccga gtatgtgtcc agctggtacc agggtcgcgg tgagctcagg ccccaactcc
     4681 acggtgtgat cgaagtcgac cgggacgatc ctctcgagcg cacgctcggc atcctcaacc
     4741 gtgtacagag cctgcggatg ctcgtggcgg gagaaaccgt gtcgggcggc gaacttcgcc
     4801 tcctcctcca acaggtgcgc gctgtcgaga agcagcagac cgagcaactc ggccgtgccg
     4861 tgcgtgacgt ggatcggacc ggcaaatccg tcccgcacga gtgcgggcag gtagccggag
     4921 tgatcgaggt gtgcgtggga gacgaccacc gagtcgatgg aggagggatc gatcgggaac
     4981 tgcgcccgat tgcgttcccg aagcaccttg tagccctgga agaggccgca gtccacgagg
     5041 atccgtctct gctgggccgt cacgaggtag cgcgatccgg tgaccgtgtc cgccgcaccg
     5101 aggaactgca gtgttgccgc cgtgtctgac atggtgcctc ctgtcgcggc gtggcgtcgc
     5161 cgttgtccca gtgtgcgccg aggagtggtc tcgcgacagt gacctttggc ctaccgctag
     5221 tatttactcg gcttcaggga ccaatccggg ttcgctcgcc tcacctagaa acaacctcgt
     5281 gaggtctgcc gtgtcatcca accctctcgc acccgcgtgg ctcgtcgagc ccgccgatgc
     5341 caacgcgctg cctgctgccg tgtggtcgca caacgcgcgg cgctccgcgg gcggcgaact
     5401 ggagatcgcg ggcgtgtccg cctcagcgct ggtggccgag ttcggtacgc ccgtgtacgt
     5461 ggtcgacgag gccgacgccc gcagtcgtgc cggcggcatc cgcgaatcgt tcgaccgcga
     5521 gttcgcgcga gtgggcagca gcgccaaggt ctactacgcg ggcaaggcgt tcctgtgcac
     5581 cgaggtcgcg cgctgggtca ccgaggcggg cctcaacatc gatgtcgcga gtggcggcga
     5641 gatggcggtc gcgctcgcgg caggcgtcga cccggcgcgg ctggggctgc acggcaacaa
     5701 caagagtctt tccgagatcg accgtgccgt gggtgcgggc gtcggtgcca tcgtgatcga
     5761 cagcgtcgtc gagatcgagc gcgtcgccac agcagcctca cggcacggtc gaacccagcc
     5821 ggtgcgcctg cgcatcaaca gcggggttca cgcatcaacc cacgaatacc tggcgacggc
     5881 ccgcgaggac cagaagttcg gtattcccct cgcggatgcc gcggcagcag tagccctcat
     5941 ccgcgcccag ccgtcgctga ccttcctcgg tctgcactcg cacatcggtt cgcagatctt
     6001 cgagtcggac ggcttcgcgg aggccgcgca ccgcctgttc gctctgcatg ccgagctgct
     6061 gaagggcggg ccggttcccg agctgaacct cggcggaggg ttcggcatcg cctacacgag
     6121 cgtcgacgat ccgctcgaca tcgatgagat cgcccggcgc ttcgccgaca tcgtctccca
     6181 gggtgctgcg gagctcggca tccccgttcc ggtcgtggcg atcgagccgg gtcgtgcggt
     6241 gatcggcccg tcgacgacga cgctctacac ggtgggcacg atcaaggatg tgctcgtgtc
     6301 ggtggtcgag cctgtcgaga cctgcagcga ggctcatgcc gagatctcga cgagctcgat
     6361 cgcaggcacc
//
LOCUS       DAOSOU010000113         6318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12441_length_6318_cov_5.356538, whole genome shotgun sequence.
ACCESSION   DAOSOU010000113 DAOSOU010000000
VERSION     DAOSOU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12441_length_6318_cov_5.356538"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="PLW15_07230"
     CDS             <1..166
                     /locus_tag="PLW15_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT34221.1"
                     /translation="GLAEIPKPADASDALALAICHAWRGQALVAVGAPLTPAQRAWHQ
                     AESAARRVRS"
     gene            163..777
                     /gene="ruvA"
                     /locus_tag="PLW15_07235"
     CDS             163..777
                     /gene="ruvA"
                     /locus_tag="PLW15_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT34222.1"
                     /translation="MISSVRGTVLAAVGTTVVIEVGGVGLNVQVTPQHALSLRVGSEA
                     TVRTALIVREDDLSLYGFSEADELTVFDLLRGVTGVGPKSAMGVLAAMSPGEIAQAVA
                     VEDDAAFRKVSGIGPKTAKLIVVSLAGKLLVVPTARASATSRSSTVSENVAIALTGLG
                     WPERVAAQAVEDALLSASDSDRESVPALLRLALAALGPQAVNSQ"
     gene            774..1817
                     /gene="ruvB"
                     /locus_tag="PLW15_07240"
     CDS             774..1817
                     /gene="ruvB"
                     /locus_tag="PLW15_07240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT34223.1"
                     /translation="MTEPESIIRPTPESEAELAFEGALRPRSLAEFIGQTKVRGQLQL
                     LLQAAAMQNRTPDHILLAGPPGLGKTTLAMIVAHESGRPLRMSSGPAIQHAGDLAAVL
                     SSLVPGEVLFIDEIHRMARSAEEMLYLAMEDFRIDIMVGKGAGATSIPLDLSPFTLVG
                     ATTRSGLLPNPLRDRFGFTAHLEFYDDAELEQVLERAARLLELDIDKAALAEIAGRCR
                     GTPRIANRLLRRVRDYALVKGSGAGIDAVHAALELYDVDELGLDRLDRAVMHTVLSRF
                     DGGPVGLNTLAVSVGEESETIESVVEPFLVRIGLLTRTPRGRVASRAAWKHFGMTPPR
                     AEHATLGLFGDDL"
     gene            1884..2333
                     /locus_tag="PLW15_07245"
     CDS             1884..2333
                     /locus_tag="PLW15_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT34224.1"
                     /translation="MDPLTIGMLVILAVLVFFMFRNSRKRKAQQEELRSQIVPGAEIM
                     TNFGLFGTLKSMDDLTKVAEVEVAPGTVVRVHSQTIAKVVTADDIDPNAPRSVEEAMA
                     IANREAEERDRVKLNEDSAIPLAEPAYGERVTDEAKKPRRTTKKAGE"
     gene            2440..4149
                     /gene="secD"
                     /locus_tag="PLW15_07250"
     CDS             2440..4149
                     /gene="secD"
                     /locus_tag="PLW15_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOT34225.1"
                     /translation="MARSTPVKKAWRSLTWLGVIIVGLIAINAAGVIFGGSSWAPKLG
                     LDLEGGTQIRLAPKLESGETVSQEQLNQAVSIIRQRIDAAGVSEAEISTEGSQNIVVS
                     IPGVPDEETLRRIEASAKLEFRPVIFTDVAATSAADGSTPTPVPTEGLSTTPTASPTD
                     ASDPSWVTEALYAEYQNFDCASLDQANTNVAPADEPLITCETDGSYKYILGPVEVTGD
                     TISDATSGLIMNSQGVSTGQWGVFITFNDEGTQKFAKVTERLYGFPQTDPRNRFAIVL
                     DGRVISAPTTQGIITDGKPQISGSFTQQSAKTLADQLKFGALPIGFTVQSSEVISATL
                     GSTQLFSGLIAGLIGLVLVVIYSLFQYRLLGLVTVASLVIAAVLTYLVIILLSWRYGY
                     RLSLAGVAGLIVAIGFTADSFIVYFERIRDELRDGKSLVSSVESGWKRALRTIYAAKA
                     VNLLSAVVLFVLAVGNVQGFALTLGITTVIDVLVVILFTHPILQLMATTRFFSSGHPL
                     SGLDPTALGAVYRGRAQFRAPVTTAKKDGASREAARRQTIAERKAAELSSGSSTKTDG
                     GES"
     gene            4149..5138
                     /gene="secF"
                     /locus_tag="PLW15_07255"
     CDS             4149..5138
                     /gene="secF"
                     /locus_tag="PLW15_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOT34226.1"
                     /translation="MASFAKFGNDLYTGERSFNFVGRRRIWYTIAFVMIGLSILLPIL
                     RGGFVFGIEFSGGSEFQISGVAHVDQQVASDAVTSVVADATPRVSIVGTDSVRVQTEQ
                     LTTDQSTAVRDSLAAAYDVPADQVTSSFIGPSWGADITGAAVRALIVFVALAAILMAI
                     YFRTWKMSLAAIVSLFHDMIITVGVYGAFGFEITPAAVIGFLTILGYSLYDTVVVFDK
                     IRENTSEGSGRTFAESVNLAVNQTLVRSINTGVVAALPVGAILFIGTTLLGAGTLRDI
                     SLALFVGILVGTYSTIFIGAPFYSQLRENEPEIAKSTKKVVANTSRSTTGAVR"
     gene            5143..5481
                     /locus_tag="PLW15_07260"
     CDS             5143..5481
                     /locus_tag="PLW15_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT34227.1"
                     /translation="MTSAAGAGATEIEVEDAIALVADGGYLLDVREQNEWDAGHAPGA
                     HLLPMSTIQERVAEVPDDRTVYVVCHSGARSERVTAYLRSLDLEAINVAGGMMAWRAA
                     GGEVVQSSAT"
     gene            5504..>6318
                     /locus_tag="PLW15_07265"
     CDS             5504..>6318
                     /locus_tag="PLW15_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT34228.1"
                     /translation="MTETTQSAASLRTLLPRIFSRAQPSGAVDRLIKTVRTHHPKADI
                     ALIERAYTAAEKAHSGQKRKSGEPYITHPIAVAQILADLGIGAKTLAAALLHDTVEDT
                     AYTLEMLRKDFGEEIAMLVDGVTKLDKVKYGDSAQAETVRKMIVAMSKDIRVLVIKLA
                     DRLHNARTWGFVEEASATRKAQETLEIYAPLAHRLGIQAIKLELEDLSFAVLQPKLYV
                     EIESLVSKRTPQREQFVQQVIDAVNDDLKAAKIKGSVAGRPKQYYSIYQKMIVR"
BASE COUNT         1085 a         2113 c         2004 g         1116 t
ORIGIN      
        1 gggtctcgcc gagattccca agccggccga tgcgtccgac gccctggctc tcgccatctg
       61 ccacgcgtgg cgcgggcagg cactggtggc tgtcggcgct ccgctgactc cggcccaacg
      121 ggcgtggcac caggcggagt ccgctgccag gagggtgcgc tcatgatctc gtcggttcgg
      181 gggaccgttc tcgcggccgt cggaacgacc gtcgtgatcg aggtcggtgg cgtcggtctg
      241 aacgtacagg tcactccgca gcacgcactg tcgcttcgtg tcggtagtga ggcgacggtg
      301 cgcacggcgc tgatcgtgcg ggaggacgac ctctcgctct acggtttctc ggaggccgac
      361 gaactcaccg tcttcgatct gctccgtggg gtgacgggtg tcggcccgaa gagcgcgatg
      421 ggcgtcctcg ccgccatgag ccctggcgag atcgcgcagg ccgtcgctgt ggaggacgat
      481 gccgcgttca ggaaggtgtc cggcatcggg cccaagacgg ccaagctgat cgtcgtgtcg
      541 ctggcgggca agctgctcgt ggtgcccacg gcacgggcat ccgccacgtc ccgctccagc
      601 accgtcagcg agaacgtggc cattgctctg accggactcg gctggccaga acgtgtcgct
      661 gcgcaagctg tcgaggatgc cctgctcagc gcctctgact ccgatcgtga atcggtgccg
      721 gcgctgctca ggctcgccct agcagcactc gggccgcagg cggtgaactc gcagtgaccg
      781 aaccggaatc gatcatccgc ccaacccccg agtccgaggc cgagctggcc ttcgagggtg
      841 ccctgcggcc gcgctcgctc gccgagttca tcggtcagac caaggttcgc ggtcagctcc
      901 agcttctgct gcaggctgcg gcgatgcaga atcgcacccc cgatcacatc cttctggctg
      961 gcccacccgg gctcggcaag accaccttgg cgatgatcgt cgcgcacgag agcggtcgac
     1021 cgctgcgcat gtcgagcggt ccggccatcc agcacgctgg cgatctggcc gccgtgctct
     1081 cgtcactggt gcccggcgag gttctcttca tcgacgagat tcaccgcatg gcgcgctcgg
     1141 ccgaggagat gctctacctc gcgatggagg acttccgcat cgacatcatg gtcggtaagg
     1201 gcgcgggggc aacgtcgatt ccactcgacc tctcgccgtt cactctcgtg ggggcgacga
     1261 cgcggtccgg ccttctgccc aacccgctgc gcgacaggtt cggattcact gcccacctcg
     1321 agttctatga cgacgcggag ctggagcagg tactcgagcg tgcagcacgg ctgctcgagc
     1381 tcgacatcga caaggcggca ctcgccgaga tcgccgggcg ctgcaggggc accccgcgta
     1441 tcgccaaccg gctgctgcgc cgagtgcgcg actacgcgct cgtcaagggg tccggtgccg
     1501 gtatcgacgc cgttcacgcg gccctcgagc tgtacgacgt ggatgagctc gggctcgatc
     1561 gcctggaccg ggcagtgatg cacaccgtgc tcagtcgttt cgacggcgga ccggtgggac
     1621 tcaatacgct cgctgtctcg gtgggcgagg aatcggagac gatcgaatcg gtggtcgaac
     1681 cgttcctggt gcgcatcggc cttctgacgc gcactccccg cgggcgggtc gccagccgag
     1741 cagcatggaa gcacttcggg atgacgccgc cccgagctga gcacgccacc ctggggctct
     1801 tcggggatga cctataattc tcaaggaccg ctcagcgagc ttcactaggc ttgccggtca
     1861 gactccctct cggaaggtaa cccatggatc cgttgactat tggaatgctc gtcattcttg
     1921 cggtgcttgt tttcttcatg ttccgcaaca gccgcaagcg caaggctcag caggaggagc
     1981 tgcgttcgca gatcgttccc ggtgccgaga tcatgaccaa cttcggcctg ttcggaaccc
     2041 tcaagtcgat ggatgacctg accaaggtcg ccgaggtcga ggtcgccccc ggcaccgtcg
     2101 tgcgcgtgca cagccagacc atcgccaagg tcgtcaccgc cgacgacatc gacccgaacg
     2161 cccctcgctc cgtcgaggag gccatggcca tcgccaaccg cgaggccgag gagcgcgacc
     2221 gcgtcaagct caacgaggac agcgccatcc cgcttgctga gcccgcctat ggcgagcgcg
     2281 tgaccgacga ggcgaagaag ccgcgtcgca cgacgaaaaa ggccggcgaa taaccactac
     2341 ccttgggtgc tagccaccgc gccctcaccg gccgtggctg gcactttcgg gtgcccgcgc
     2401 acccgacgca aacccacaga gaaagctgag taatagccgg tggccagatc tacccccgtg
     2461 aagaaggcgt ggcgttcgct cacgtggctc ggcgtcatca tcgtgggcct catcgcgatc
     2521 aacgccgccg gcgtgatctt cgggggcagt tcgtgggccc ccaagctcgg tcttgacctc
     2581 gagggcggca cccagatccg tctcgcgccg aagctcgaga gcggcgagac cgtcagccag
     2641 gagcagctca atcaggctgt gtcgatcatc cggcagcgca tcgacgccgc gggtgtgtcc
     2701 gaggcggaga tcagtacgga gggctcgcag aacatcgtcg tctcgatccc cggtgtgcct
     2761 gacgaggaga cgctgcgccg cattgaggcc tcggccaagc tggaattccg acccgtgatc
     2821 ttcacggatg tcgcagccac gagtgccgcc gacggctcga cgcccacccc ggtgccgacc
     2881 gaggggctct cgaccacacc caccgccagc cccacggacg cctcggaccc gagctgggtc
     2941 accgaggcgc tgtatgccga gtaccagaac ttcgactgcg cgagcctcga tcaggcgaat
     3001 accaatgtcg ctcccgccga tgaacccctc atcacttgtg agactgacgg tagctacaag
     3061 tacatcctcg gcccggttga ggtcaccggt gacacgatct ccgacgcgac gagcggcctg
     3121 atcatgaaca gccaaggtgt cagcaccggc cagtggggcg tgttcatcac gttcaacgac
     3181 gagggaaccc agaagttcgc caaggtgacc gagcgtctct acggcttccc gcagaccgac
     3241 ccgcgcaacc gattcgcaat cgtcctggat ggccgggtga tctctgcgcc gaccacgcag
     3301 ggcatcatca ccgacggcaa gccgcagatc agcggctcgt tcacgcagca gagcgcgaag
     3361 accctcgccg accagctcaa gttcggcgcc ctgccgatcg gcttcacggt gcagagctcg
     3421 gaagtgatct cggcgacgct cggatccact cagctgttca gcgggctcat cgccggcctc
     3481 atcggcctcg tgctcgtcgt gatctactcg ctgttccaat accgattgct gggtctcgtc
     3541 accgtcgcat ccctcgtgat cgctgcagtg ctgacctatc tcgtcatcat ccttctgtcg
     3601 tggcgctacg gctaccgcct gtcgctggcc ggtgtcgcag gcctcatcgt cgcgatcgga
     3661 ttcaccgcgg actccttcat cgtgtacttc gaacgaatac gtgacgagct gcgtgacgga
     3721 aagagtctcg tctcctccgt ggagtccggg tggaagcgtg cgctgcgcac catctacgcg
     3781 gccaaggcgg tcaacctgct gtcggcggtc gtgctcttcg tgctcgccgt cggcaacgtc
     3841 cagggcttcg ctctgacgct gggtatcacc accgtgatcg acgtgctcgt cgtgatcctc
     3901 ttcacccacc cgatactgca actcatggct acgacgcggt tcttctcgag cgggcatccg
     3961 ctcagcggac tcgaccccac cgctctgggt gccgtctatc gtgggcgcgc gcagttccgt
     4021 gccccggtca ctacggcgaa gaaggatggc gccagccgcg aggcagcgcg ccgccagacc
     4081 atcgccgagc gcaaggccgc agagctcagc tccggatcca gtacgaagac cgacggaggg
     4141 gagtcctgat ggccagtttc gccaagttcg gcaatgacct gtacacgggc gagcgctcgt
     4201 tcaacttcgt cggccgccgt cgcatctggt acacgatcgc tttcgtgatg atcggcctct
     4261 cgatcctgct tccgatcctg cggggcggat tcgtcttcgg tatcgagttc agcggcggtt
     4321 ctgagttcca gatctccggc gtcgcccacg tcgatcagca ggtcgcgtcc gacgccgtca
     4381 cgagtgtggt ggcggatgcc acgccgcgtg tctcgatcgt cggcaccgat tctgtgcggg
     4441 tgcagaccga gcagctcacg accgaccagt cgaccgccgt tcgcgattcg ctggccgcgg
     4501 catacgacgt tcccgccgac caggtgacat cgtcattcat cggcccatcg tggggtgccg
     4561 acatcaccgg cgcagcagtc cgggcgctca tcgtcttcgt cgccctggcc gcaatcctga
     4621 tggcgatcta cttccgcacg tggaagatgt cgctggcagc gatcgtgtcc ctgttccacg
     4681 acatgatcat caccgtcggt gtctacggcg ccttcggctt cgagatcacc ccggcggcgg
     4741 tgatcggttt cctcacgatt ctcggctatt cgctctacga caccgtcgtg gtgttcgaca
     4801 agattcgcga gaacacgagt gagggatccg gtcgcacatt cgccgagtcg gtcaacctcg
     4861 ccgtcaacca gactctggtg cgatccatca acacgggtgt tgttgcggca cttccggtcg
     4921 gggcgattct cttcatcgga acgaccctgc tgggcgcggg aacgctgcgt gacatctcgc
     4981 tcgcgttgtt cgtgggaatc ctggtgggca cgtactcgac gatcttcatc ggtgcgccgt
     5041 tctactccca gctccgtgag aacgagcctg agatcgccaa gtcgaccaag aaggtcgtgg
     5101 cgaacacgtc acgctccacc acgggtgcgg ttcgctgatc tcatgacatc cgctgccggc
     5161 gcaggggcga ccgaaatcga ggtcgaggac gcaattgcgc tcgtggcgga cggcggctac
     5221 ctgctcgacg ttcgggagca gaatgaatgg gacgcggggc atgcacctgg cgcccacctg
     5281 ctgccgatgt cgacaatcca ggagcgggtc gctgaggtcc ccgacgatcg aaccgtctac
     5341 gtggtctgcc acagcggggc tcgctccgaa cgggtgaccg cgtacctgcg ttctctggat
     5401 ctcgaagcca tcaacgtcgc gggcgggatg atggcctggc gggccgccgg tggcgaggta
     5461 gtgcaatcct ccgcgaccta gaatcagtgg tggaggtgcg cgcatgacgg agaccacgca
     5521 gagcgccgcc tcgctgcgca ccctgctgcc tcgcatcttc tcgcgcgccc agccttcggg
     5581 tgcggtcgac cggctcatca agacggtgcg cacgcatcat ccgaaagcgg atatcgccct
     5641 gatcgagcgg gcctacaccg ctgcggagaa ggcgcattcc gggcagaaac gcaagagtgg
     5701 cgagccctac atcacccacc ccatcgcggt ggcccagatc ctcgccgacc tggggatcgg
     5761 agccaagaca cttgcggcgg cgctgctgca cgacaccgtc gaggacaccg cctacaccct
     5821 cgaaatgctc cgcaaggact tcggcgaaga gatcgccatg ctcgtggatg gcgtgacgaa
     5881 gctcgacaag gtcaagtacg gtgacagcgc ccaggccgag accgtacgca agatgatcgt
     5941 ggcaatgtcc aaggacatcc gggtgctggt gatcaagctc gccgaccgcc tgcacaacgc
     6001 gcgcacctgg gggttcgtgg aggaggcatc cgcgactcgc aaggcgcagg agactctgga
     6061 gatctacgca ccgctcgcgc accgcctcgg tattcaggcg atcaagctcg agcttgagga
     6121 cctgtcgttc gcggtgctcc agcccaagct ctacgtcgag atcgagagtc tggtctccaa
     6181 gcgcacgcct caacgcgagc agttcgtgca gcaggtcatc gatgccgtga acgacgatct
     6241 caaggcggcg aagatcaagg gcagcgtcgc cggccggccg aagcagtact actcgatcta
     6301 ccagaagatg atcgtgcg
//
LOCUS       DAOSOU010000114         6311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12454_length_6311_cov_4.844469, whole genome shotgun sequence.
ACCESSION   DAOSOU010000114 DAOSOU010000000
VERSION     DAOSOU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12454_length_6311_cov_4.844469"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLW15_07270"
     CDS             <1..793
                     /locus_tag="PLW15_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT34229.1"
                     /translation="LNKELGLDKPLLVQYVTYLGHLLTGDLGNSIKYSAPVTEIIAAP
                     LGNTVMLVVLAVIIGGVIGLVFGILSGNWPGSVLDRIVSGLIAVAVSTPGFVIAIFLS
                     LFVGLSLGWFPSIGYTAPTDDFGDFLYHAFLPSLALSFGFIAIIGRVTRTSVLSVRRE
                     DWVRTARGMGLSGGRVFRRHVFRNAVNPVVTVAGLQFGALLGATVLIEQVFNWPGIGS
                     TLLNAINGRDYPLVQGIVIVFAVVFIVTSLLVDLLYPLLDPRVER"
     gene            799..1713
                     /locus_tag="PLW15_07275"
     CDS             799..1713
                     /locus_tag="PLW15_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT34230.1"
                     /translation="MTRTFRTLWQKSSPGLFITTCVVFLAVCFIFIYPAVTSVDPLAQ
                     DIPHAFAPVGTAGHLLGTDNLGRDTFVRLVVGLRTELIVCAAGTLIAAIIGTLIGILA
                     AYFRGVTDLALMRVVDIFLAFPHLVFAMLIVSLYGAGDLTVVAVCAIAFFPSFARLTY
                     GEVLSLRQALYVESAELFGGSHWQIIRGPLLRGVGPLLLAQAFLTLAFAVGLESGLSF
                     LGLGITPPRPSLGLMIASGQQYFTQDASQLLIPSITLVVLLVVLGYVADWVRDLLDPR
                     GVTRRRRLRRGDAVTRTEVISLREEVGA"
     gene            1710..3437
                     /locus_tag="PLW15_07280"
     CDS             1710..3437
                     /locus_tag="PLW15_07280"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT34231.1"
                     /translation="MNALDETLLSVTDLTIAVAGTEVVHGVSFTVRPGEVVALVGESG
                     AGKSMTAMAIPNLLPAGSERGGSIRFGGTELLELSESGMRRYRGGEIACVYQEPMTAL
                     NPLHTVGDFLTESIDAHGEGQGRRREPQELLDLVGLGSFDDLLQRYPHQISGGQRQRV
                     MAAGAVAWDPKLLIADEPTTALDVTTQRNLLAMFRNLVKREQMAMIFVTHDMGVVADI
                     ADSVVVMRNGRVVEAGDVFSIFASPQHEYTRSLLSAARALHGSEPDAEAVPAQAPPVV
                     VPPPAVAPAPAVAPAVALEVRDVVVEYRSTGRRRRSSGRKAAVQGASLSVARGQTLGI
                     VGESGSGKSTIARAILGLAPVTGGSVSLAGTEMVGLRGRRRRQAMARLGVVFQDPTSS
                     LDPSLPLWRIVTEPLWRSGRVRDARSLRARAGELLGDVGLDPTWIDRRRHELSGGQRQ
                     RVAIARAVSHEPDVLIADEPTSALDVTVQVTVLELLSRLQRTHNFACIFISHDLYVVS
                     TISDRVVVMKDGRIVEDGSTDEVIHDPENEYTRTLLAAIPVPDPVVQRARRSALDEET
                     DHTAGGHER"
     gene            3434..5035
                     /locus_tag="PLW15_07285"
     CDS             3434..5035
                     /locus_tag="PLW15_07285"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOT34232.1"
                     /translation="MKAQYRVAGSYGAKQVSTGSRGMVVSSEPIAVEAGAAVLREGGN
                     ALDAALAVAATQLVTEPHMTSITGGMSLVYRHAATGAAAYLSGNVNAPLAGLPDFTGA
                     DLTRARGVPVPGWWPSFLAAHERFGSVALARLLEPAIDVAREGFAVSPYLFGEIYHAR
                     ENLGRNPQSREMFFRAGSLIGPGQALVQERVARTLERLRDEGMSYYLGDFARAFCASS
                     DSDGGVITEADFEAYEPEWAAPVRGTYRGFEVVGSAPPDDGGLQLVEAFNILENIDLA
                     ALGPASRSPETLTALIAAHNAVYYAAPREKRFRDSERIMDILLSKQYGRQRFALLDTL
                     ASPITAAPPTPGTIHLSVVDADHNVATLTHSHMASPWVNGLYAEGFQLAGGGSFFQRV
                     MPQPGERATVYLAPSMVLKDGRPVIASGSPSVSLVACVLQNLVNLMDFGMSIEQSVAE
                     PRFGARPHDPARGWVPGTTLESGYADGVLDAVLRWAGGNRLWTREIGPWNSMTGNFDG
                     ITIDPVTGEMRSCGDPRRMGVAIAV"
     gene            5469..5928
                     /locus_tag="PLW15_07290"
                     /pseudo
     CDS             5469..5928
                     /locus_tag="PLW15_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029622100.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6124..>6311)
                     /locus_tag="PLW15_07295"
     CDS             complement(6124..>6311)
                     /locus_tag="PLW15_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOT34233.1"
                     /translation="LNVIAMVRMVHVPDTRGYVNRRHTEGKTDREIRRILKRYLARSI
                     YRHLNAASNSKSGVDEI"
BASE COUNT          916 a         2146 c         2110 g         1139 t
ORIGIN      
        1 cctgaacaag gagctgggcc tggacaagcc gctgctggta cagtacgtga cctacctcgg
       61 acacctgctc accggcgatc tcggcaactc gatcaagtac tcggcccccg tcaccgagat
      121 catcgccgct ccgctcggca acaccgtgat gctcgtggtg ctcgccgtga tcatcggcgg
      181 ggtcatcggg ctggtcttcg gcatactgtc gggcaactgg ccgggcagcg tcctggaccg
      241 gatcgtctcc ggtctcatcg ccgtcgccgt ctccaccccc ggattcgtga tcgcgatctt
      301 cctctcgctg ttcgtcggac tgagcctggg atggttcccg tccatcggct atacggcccc
      361 cacggacgac ttcggagact ttctctatca cgcgttcctt ccgagcctgg cgctgtcctt
      421 cggcttcatc gcgatcatcg ggcgcgtcac gaggacctcc gtgctgtcgg tgcggcgcga
      481 ggactgggtt cgcactgccc gcggcatggg gctctccggg gggcgcgtct tccgccgtca
      541 cgtgttccgc aacgccgtca accccgtggt cactgtggcg ggcctgcagt tcggcgcgct
      601 tctgggcgcc accgtgctca tcgagcaggt gttcaactgg ccggggatcg gcagcactct
      661 gctcaacgcg atcaacggcc gcgactatcc cctggtgcag ggcatcgtga tcgtcttcgc
      721 ggtcgtcttc atcgtgacga gcctgctcgt cgatcttctc tatcccctcc tcgacccccg
      781 agtggagcgt tgatcctcat gacacgcacg ttccggactc tgtggcagaa gtccagccca
      841 gggctgttca tcacgacctg cgtggtgttc ctggcggtct gcttcatctt catctacccg
      901 gcggtgacca gtgtcgaccc cctggcgcag gacatcccgc acgcgttcgc gccggtggga
      961 accgcaggcc atctgctggg aacggacaat ctcggacgcg ataccttcgt acgcctcgtc
     1021 gtgggcctgc gcaccgagct gatcgtgtgc gcggcgggca cactcatcgc cgcgatcatc
     1081 ggaaccctga tcggcatcct cgcggcgtac ttccgcggcg tcaccgacct cgccctgatg
     1141 cgcgtcgtgg acatcttcct cgccttcccg cacctcgtgt tcgccatgct catcgtgtcg
     1201 ctgtatggcg ccggtgacct caccgtggtg gcggtctgcg cgatcgcctt cttcccgagc
     1261 ttcgcgcgcc tcacctacgg cgaggtgctg tcgctgcgcc aggcgctgta cgtcgaatcg
     1321 gccgagctgt tcggcggcag ccactggcag atcatccggg gtccgctgct gcgcggcgtc
     1381 ggaccgctgc tgctggcgca ggcattcctg acgctggcgt tcgccgtcgg cctcgaatcg
     1441 ggcctgagct tcctcggcct gggcatcacg cccccgcgtc cgtcgctggg gctcatgatc
     1501 gcctccggtc agcagtactt cacgcaggat gcgtcgcagt tgctgatccc gagcatcacg
     1561 ctcgtcgtgc tgctggtcgt gctgggctac gtcgccgact gggtacggga tctgctggat
     1621 ccgcgcggcg tgacccgacg gcgtcgcctg cgccgggggg atgccgtgac gcggaccgag
     1681 gtcatctcgt tgcgagagga ggtcggcgca tgaacgcgct cgatgagacc ctgctgagcg
     1741 tgacggatct gacgatcgcg gtcgcgggga ccgaagtggt ccacggcgtc tcgttcaccg
     1801 tccgcccggg cgaggtcgtc gcgctggtgg gcgagtccgg cgccgggaag tcgatgaccg
     1861 ccatggcgat cccgaacctc ctgccggccg gctccgagag aggcggatcg atccggttcg
     1921 gtggcacgga gcttctggag ctgtccgagt cgggaatgcg ccgctatcgc ggaggcgaga
     1981 tcgcctgcgt gtatcaggag ccgatgacgg cgctgaatcc gctgcacacc gtcggcgact
     2041 tcctgacgga gtcgatcgac gcgcacggcg agggtcaggg gcggcgccgc gagccgcagg
     2101 agctgctgga tctcgtgggc ctcggctcgt tcgacgacct tctgcagcgc tatccgcatc
     2161 agatctccgg cggccagcga cagcgggtga tggccgccgg agcggtggcc tgggatccga
     2221 agctgctgat cgcggatgag cccaccacgg cgctggacgt cacgacgcag cgcaacctgc
     2281 tcgcaatgtt ccgcaacctc gtcaagcgcg agcagatggc gatgatcttc gtgacgcacg
     2341 acatgggcgt cgtcgccgac atcgcggact ccgtggtcgt catgcgcaat ggccgggtcg
     2401 tggaagccgg tgacgtcttc tcgatcttcg cgtcgccgca gcacgagtac acccggtcgc
     2461 tgctctcggc ggcccgggcg ctgcacggtt ccgagccgga cgcggaggcc gtgcccgcgc
     2521 aggccccgcc tgtggtcgtg cccccgcccg cggtggcgcc cgcgcccgcg gtggcgcccg
     2581 cggtcgcgct cgaggtccgc gatgtggtcg tcgagtaccg cagcaccggg cgccgccgtc
     2641 gatcgagcgg ccgcaaggcc gccgtgcagg gcgccagtct gtcggtggct cgtggccaga
     2701 cgctggggat cgtcggggaa tccggatccg gcaagtcgac gatcgctcgg gcgatcctcg
     2761 ggctcgcgcc cgtcacgggt ggctcggtgt ccctcgccgg caccgagatg gtcggactgc
     2821 gcggccgacg gcgacgccag gcgatggcgc ggctcggggt cgtgttccag gaccccacct
     2881 cgtcgctgga tcccagtctg ccgctgtggc ggatcgtgac cgagccgctg tggcgcagcg
     2941 gcagagtgcg tgatgcgcgg tcgctgcgcg cccgggccgg cgagctgctc ggcgacgtcg
     3001 ggctggatcc gacgtggatc gaccggcgcc ggcacgagct gtcgggcggt cagcgccagc
     3061 gcgtcgcgat cgcccgcgcg gtctcgcatg agccggacgt gctcatcgcc gacgagccca
     3121 cttctgcgct ggacgttacg gtgcaggtga ccgtgctcga gctgctgtcc cggctgcagc
     3181 gcacgcacaa cttcgcctgc atcttcatca gccacgacct gtacgtggtg tcgacgatct
     3241 cggatcgcgt cgtcgtgatg aaggacggcc ggatcgtcga agacggatcg acggacgagg
     3301 tgatccacga ccccgagaac gagtacacca gaactcttct cgccgccatt cccgtgccgg
     3361 atcccgtcgt ccagcgcgct cgccgcagcg cactggacga ggagaccgac cacaccgcag
     3421 gaggacacga acgatgaagg cgcagtaccg agtcgcagga tcctatggcg ccaagcaggt
     3481 ctcgaccggg tcgcggggga tggtggtcag ctcggagccg atcgccgtag aagccggcgc
     3541 tgcagtgctg cgcgagggcg ggaacgcgct ggatgcggcc ctcgccgtcg ccgccaccca
     3601 gcttgtcacc gaaccgcaca tgacctcgat caccggcggc atgtcactgg tctaccggca
     3661 tgccgcgacc ggggcggcgg cgtacctgtc ggggaacgtg aatgcgccgc tggcgggatt
     3721 gcccgacttc acgggcgcgg acctgacccg cgcgagaggt gtgccggtcc ccggctggtg
     3781 gccgtccttc ctggccgccc acgagcggtt cggatccgtc gccctggccc ggctcctcga
     3841 gccggcgatc gatgtcgcgc gcgagggctt cgccgtctcg ccgtatctgt tcggcgagat
     3901 ctatcacgcc agggagaacc tcggacgcaa tccccagtcg cgtgagatgt tcttccgggc
     3961 gggctccctg atcggtccag ggcaggcgct cgtgcaggaa cgtgtcgcgc ggaccctgga
     4021 acgcctgcgc gacgagggga tgtcgtacta tctcggagac ttcgcccgtg cgttctgcgc
     4081 gtccagcgac tcggacggcg gcgtcatcac cgaggcggac ttcgaagcct acgagccgga
     4141 gtgggctgcc ccggtgcggg gcacctaccg aggattcgag gtggtcggat ccgccccgcc
     4201 ggatgatgga ggactgcagc ttgtcgaggc gttcaacatc ctggagaaca tcgatcttgc
     4261 cgccctcggt cctgcgagcc ggtcgcccga gacgctcacc gcgctgatcg cggctcacaa
     4321 cgccgtctac tatgccgcgc cgcgggagaa gcggttccgc gacagcgagc ggatcatgga
     4381 catccttctg tcgaagcagt acggacgcca gcgcttcgcg ttgctggaca cgctcgcgtc
     4441 tccgatcact gcggccccgc cgacccccgg aacgatccat ctctcggtcg tggacgcaga
     4501 tcacaacgtc gcgactctga cccattcgca tatggcgtcg ccgtgggtca acggcctcta
     4561 cgccgaggga ttccagctcg ccggtggcgg atcgttcttc cagcgcgtca tgccacagcc
     4621 gggggagcgc gccaccgtgt atctcgcgcc gagcatggtg ctcaaggacg gcaggcctgt
     4681 catcgcatcc ggatcgccct ctgtgtcgct cgttgcgtgc gtgctgcaga atctggtgaa
     4741 cctgatggat ttcggcatga gcatcgagca gagcgtcgcc gagccccgat tcggcgctcg
     4801 cccgcacgac cccgctcgtg gatgggtgcc cggcacgacg cttgagtcgg gatacgccga
     4861 cggtgtgctc gatgcggtgc tccgctgggc cgggggcaac cgtctctgga cacgcgagat
     4921 cggcccgtgg aactcgatga cgggaaactt cgacggcatc acgatcgacc cggtgaccgg
     4981 cgagatgcgc tcctgcggag atcctcggcg gatgggggtg gcgatcgccg tctgagcgtc
     5041 gtcgttgccg agcgcgccgt gtgggtcggc gtgctcggct ggtgtcgtcc tcgcgcgacc
     5101 atcactgcgg agtgcgatgc gattgagcgc cgtcctcctg ggggacgatg acgctcccgg
     5161 tcggtctcgc caacctggca tctaagctgt ccacgaacta cccgatggtg ttcgcgggta
     5221 tgacgctggc gtcggtgccg atgatcgtat tcttcttcgc ggcgcagtgc ttcttcgttc
     5281 gcatcttcga ctgcgacggg gtgctggtcg acagtgagac cgtcagcgcc ggggtcagtc
     5341 agcgggtgct ggccgacctc ggcgtcacgg tgccgctcga ggagatcctc gaccgcttcg
     5401 ccggcgcatc cccggaagta tttgccgcag ctctcgtgtc cccacgattg gccgagcagc
     5461 tggccgcgct gcgccggcaa cgtacgagat cgccgtcgag gtcgaggtcg aggtcgagta
     5521 gatcgtcgac gcgtaccctc ttcagccggt ctgacgagca tgcccggagt cggcgtcagg
     5581 gccgcagccc ggctcctcac cgaagtcacc accaagatgt tccccacctc cgggcacctc
     5641 accgcctacg ccggcctcgc acctattacg aggcgatccg ggacctcgat ccgcggagag
     5701 tatccgtcaa ggcgcgggaa caagatcttg aaacggacca tgttcctctc cgcattcgcc
     5761 gcactccgcg accccgaatc gcgcgcttgc ttcgatcgaa agatcgccca gggcaaacgt
     5821 cacaaccaag ccctcatcgc cctcgcaaga cgtcgatccg acgtgctcta cgcgatgctc
     5881 cgcgacggca ccctccacca atcgcaagtc gaaccgatcg cggcataggc cacgatccgc
     5941 tggcgagccc cggcaactgc ctatcgatcc gctacgaccc agccgaatcg ctcacagaag
     6001 tcctttcggc acttggggca gacccacgtg tattcgtcat ccgccgtcac tcaagcctcg
     6061 ttgagggcag catggccttt gacgtcgtcc gagatctctg cccagcggaa atcacagaag
     6121 cttctatatc tcgtcaaccc ccgatttgga atttgaggcg gcgttgaggt gtcggtagat
     6181 gctgcgggcg aggtagcgct tgaggatgcg tcggatctcg cggtcggtct tgccttcggt
     6241 gtgtcgccgg ttgacgtagc cgcgggtgtc ggggacgtgg accattcgga ccatagcgat
     6301 cacgttcagc g
//
LOCUS       DAOSOU010000115         6304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12474_length_6304_cov_7.337654, whole genome shotgun sequence.
ACCESSION   DAOSOU010000115 DAOSOU010000000
VERSION     DAOSOU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12474_length_6304_cov_7.337654"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..321)
                     /locus_tag="PLW15_07300"
     CDS             complement(25..321)
                     /locus_tag="PLW15_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34234.1"
                     /translation="MELLFVTVIGAGIGLIVRYVLPSRGTYGVLLVPAISAAATAITW
                     VALLWAGQTFDGTWIWVASLAAAVAAGIAAAVTLPRQRVDADARLFAQLSGGKA"
     gene            complement(338..562)
                     /locus_tag="PLW15_07305"
     CDS             complement(338..562)
                     /locus_tag="PLW15_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HOT34235.1"
                     /translation="MDIRIGIANSPREINFESSQSSAEIEKIVATALDTDAKFVSLKD
                     DKGKLYIVPTAALAYLEVGSEESRRIGFVA"
     gene            643..3729
                     /locus_tag="PLW15_07310"
     CDS             643..3729
                     /locus_tag="PLW15_07310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT34236.1"
                     /translation="MMGFARGRSAAPQVSMDPSQRVVIDLPDDASAAVLGAPGTGKTT
                     TLIEVVADRVLARGWGADAVLALTTSRATATRLRDAIALRLGVPTTGPMARSVNSLAF
                     EIVRDAARTAGALPPRLVSGAEQDVELASLIEGHLEEGSGPRWPEHLGPEVRRLRTFR
                     TELRELMTRATEYDVDVAQLRRLGGAHGHPEWVAAAELIEEYTQVLSYRSETRFDQAE
                     LTRYAVAAVERGELGERASALRLVVVDDLQEATETTLALLRSLAARGIAVIAFGDPDV
                     AANAFRGGEPDALGRLATVLALPDLTTLTLSTSHRQGAALRAFTSSVTARIGTAAAGR
                     QRAAIGAGADAAVPVALVQAPSPAREWSTVARALREAHLHRGVPWSQMVVVVRSGAQL
                     APVVRALAMAEVPARTTAAGTALREDFAARSLLRLVSVGVGREPLTPAVATELLLGPF
                     GGLDALALRRLRIALRAEEVAGGGNRPGDELVAEALAAPGRLASIDHRVARAAERLAE
                     TIARLGASDGSIEELLWIAWDRSGLARSWHDQALGAGITAAEANRNLDGIVALFTSAK
                     RFVELRPDKGPSLYLDEVLDAEVPEDTLSPRAFDDAVLVTTPAGTVGLEFDTVVVGGL
                     QDGAWPNLRLRGSLLAPQDLVRIVTGVESTAVDERKQVLGDELRLFALAVSRARTRVI
                     LAAVANDDEASSVFLGLAPAGTETLDPTATPLSLRAQVGKLRRMLVAGQRAGEAAASL
                     AALAVESVPGADPDDWHGLRQPSSTGPLFEGEQVPVSPSAIEKLLDSPLDWFLEKIAG
                     SDSGVIANVGTIVHWAMETAPEPTQEALWAAVEQRWSELLFEAPWLAERQRRIAMGFT
                     TALAEYLQDFQREGKTLVGAESRFTLEVDNAKLSGSIDRVEISPTGEVVIVDLKTGTP
                     ITTQSTIDEHPQLAAYQLAYTEGILDEPLAGHDGHRQGGAKLLFVKQGIGSKLYREAH
                     QAPLDTDQLEAFRERVRAAASLIAAAEFAGEAELPIYGRGDPSRLRLQRVRAVSSD"
     gene            3722..>6304
                     /locus_tag="PLW15_07315"
     CDS             3722..>6304
                     /locus_tag="PLW15_07315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOT34237.1"
                     /translation="MTEPTINADQIADALSRPRPTAQQRAVIESPLAPALVVAGAGSG
                     KTETMAGRVLWLLANRMVDPSQILGLTFTRKAAGELALRIRERIADLARVGLLGEGYD
                     PFDAPTVATYNSYANTLYRDNAAALGRESDGAVLGEAAAWQLARTTVIRTKDDRLADI
                     GKNLDPVTKATLTISRSLAENVAVPVEVQEFARRFARIVDLPPGGRGAYAQVDGWAET
                     VGALDVLVDLAAEYGDAKVRRGLVEYSDQVALALEILRRQPRVAEIERDRFRVVLLDE
                     YQDTSVVQARLLAQLFAGHPVMAVGDPNQSIYGWRGASASNLDDFATAFGATDTRFAL
                     STSWRNGRRILDVANTIVAPFVAGAKVQVDRLDASPTASDLPVDIEFLETVADEADAA
                     ARWLKARLDVRGTPPSAAMLFRTRKTQGFFLEAMRRHGVKFHVLGIGGLLAEPEVADL
                     VCALTVMNDPSAGLELVRLLAGSRWRVGVQDLWALHRLASWLRDRDYAQQRYDDELAQ
                     ALKASVAEGEGGSIVDALDFLSTAREGHTALASFTPEGLDRLRDAGRLIARLRSRGGL
                     DLPDLVAFVLQELQLDIEVSANDYRPLGASTFEAFFDAVGNYLALEEVATLGGFLSWL
                     REAEQREDLSPRPEDPEPGTVQVLTIHGAKGLEWDHVVVPRLVDDELPGRPVEGGYLG
                     WLAFGQLPWSFRGDAAELPVFAWESQTTRKDLVDEQKAFGGRVREHLLAEERRLAYVA
                     VTRARHGLLLSGSFWATQAKPRVPSVYLTELGLDLPEGPASDENPMGDDLERIIWPMD
                     PLGRRRAAVESAAAAVRDTDPAIVGPWTDDVELLLEERRRRLGAPEPVEVPTRVPASR
                     FKDYV"
BASE COUNT          874 a         2243 c         2223 g          964 t
ORIGIN      
        1 gcgcccagtc gcgcgatccg tgggttaggc cttaccgcct gagagctggg cgaacaggcg
       61 ggcatccgcg tcgacgcgct ggcggggcag agtgacggcg gcggcgattc ccgcggcgac
      121 ggctgcagcg aggctggcca cccagatcca ggttccgtcg aacgtctggc cggcccacag
      181 cagggccacc caggtgatgg cggtagccgc cgcgctgatc gcgggaacga gcagcacgcc
      241 gtaggtgccg cggctcggca gcacgtagcg cacgatgagg ccgatgccgg cgccgatcac
      301 ggtgacgaag agcagttcca tgcgtcgatc ctctcggtca ggcgacgaag ccgatgcggc
      361 gggactcctc ggagcccacc tccaggtacg caagcgcggc cgtgggaacg atgtagagct
      421 tgcccttgtc gtccttcagg ctcacgaact tggcatccgt gtcgagagcg gtggcgacga
      481 tcttctcgat ctccgcggag ctctgcgacg actcgaagtt gatctcacgg gggctgttgg
      541 caatgccgat acgaatgtcc acaggcttag ggtacgacag aactcgggag gcgattcctt
      601 gcgttctctg tcagcgcacg ccgctaccgt tgacccgtga cgatgatggg attcgcccgc
      661 ggccgctccg ccgctccgca ggtctcgatg gacccgtcac agcgcgtcgt catcgacctc
      721 ccggatgacg catccgccgc cgttctcggc gcccccggca ccggcaagac caccacgctc
      781 atcgaggtcg tcgcggaccg ggtgctcgcc aggggctggg gtgccgatgc ggttctcgcg
      841 ctcaccacgt cccgcgccac ggccacccgc ctgcgcgacg cgatcgccct gcgcctcgga
      901 gtgcccacga cggggccgat ggcgcggtcg gtcaactcgc ttgccttcga gatcgttcgc
      961 gatgccgccc gcaccgctgg ggcgttgccg ccgcggctgg tcagcggcgc cgagcaggat
     1021 gtcgagctcg cctccctcat cgagggtcac ctcgaggagg ggtccggccc gcgctggccc
     1081 gagcacctcg gtcccgaggt gcggaggctg cgcaccttcc gcaccgagtt gcgtgagctg
     1141 atgacccggg ccacggagta cgacgtggat gtcgcgcaac tgcgccgcct gggcggcgcg
     1201 cacgggcatc cggagtgggt ggctgccgcg gagctcatcg aggagtacac gcaggtgctc
     1261 tcctaccgct ccgagacgcg cttcgatcag gccgagctca cccgctacgc ggtcgcggcg
     1321 gtcgagcgcg gcgaactggg ggagcgggcc tcggccctgc gcctggtggt cgtcgacgac
     1381 ctgcaggagg cgaccgagac caccctcgcg ctgctgcgtt cgctggccgc ccggggcatc
     1441 gcggtgatcg ccttcggtga tccggatgtc gcagccaacg ccttccgcgg cggcgagccc
     1501 gacgccctcg gccgcctggc caccgtcttg gccctgccgg atctcaccac gctcacgctc
     1561 tcgacgagtc accgtcaggg tgccgccctg cgggccttca cgtcgtccgt cacggcccgc
     1621 atcggcacgg ccgccgcggg caggcagcgc gccgcgatcg gcgcgggcgc cgatgccgcc
     1681 gtgccggtcg ccctcgtgca ggcgccgagt cccgcgcgcg agtggtcgac ggtggcccgc
     1741 gcccttcgtg aggcgcacct gcaccgcggc gtgccgtggt cgcagatggt tgtcgtggtg
     1801 cgcagcggcg cgcagctggc gccggtggtg cgagctctgg cgatggccga ggttccggct
     1861 cgcacgaccg ctgcgggtac ggcgctgcgc gaggatttcg cggcccgcag tctgctgcgg
     1921 ctggtctcgg tcggtgtggg gcgagagcca ctcacgccgg ccgtcgcgac cgagttactt
     1981 ctcggcccgt tcggtgggct tgacgcgctg gccctgcgcc gcctgcgcat agcgctccgc
     2041 gccgaggagg tcgctggagg cggcaaccgg cccggagacg agctcgtcgc cgaggcactg
     2101 gccgctcccg gcaggctcgc ctcgatcgac catcgggtcg cgcgggccgc cgagcgcctt
     2161 gccgagacca tcgcccgtct cggggcgagc gacggctcca tcgaggagct gctgtggatc
     2221 gcgtgggatc gcagcggtct cgcccgtagc tggcacgacc aggcgctcgg tgccggcatc
     2281 acggcagccg aggccaaccg caacctcgac ggcatcgtgg cgctgttcac ctccgcgaag
     2341 cgtttcgtgg agctccggcc cgacaagggc ccgtcgctct acctcgacga ggtgctcgac
     2401 gcggaggtgc cggaggacac cctgtcgccc cgggctttcg acgacgccgt gctcgtcacc
     2461 acacccgccg gaactgtcgg cctggagttc gacaccgtcg tcgtcggtgg ccttcaggat
     2521 ggcgcatggc ccaacctgcg gctgcgcggc tcgctcctgg ctccgcagga tctcgtgcgc
     2581 atcgtcaccg gggtggaatc gaccgcggtc gacgagcgca agcaggtgct cggcgacgaa
     2641 ctgagactct tcgccctcgc cgtctcccgg gctcgcaccc gggtgatcct cgccgccgtc
     2701 gccaatgacg acgaggcgtc gagcgtgttc ctcggcttgg ccccggcggg caccgagacg
     2761 ctcgatccga ccgccacccc gctgtcgctg cgcgcgcagg tgggcaagct gcgccggatg
     2821 ctcgtggccg ggcagcgcgc gggggaggcc gcggcatccc tcgccgcgct cgcggtcgag
     2881 tcggtgcccg gcgccgaccc ggacgactgg cacggtctca ggcagcccag ctccaccggg
     2941 ccgctgttcg agggcgagca ggtgccggtg tccccgtcgg cgatcgagaa gctgctcgac
     3001 agcccgctcg actggttcct cgagaagatc gcgggctccg actcgggcgt gatcgccaac
     3061 gtcggcacca tcgtgcactg ggcgatggag acggcccccg agcccaccca ggaggcgctg
     3121 tgggcggcgg tggagcagcg ctggagcgag ttgctgttcg aggcgccctg gctcgccgag
     3181 cgccagcgac gcatcgcgat gggtttcacg acggcgctcg ccgagtacct gcaggacttc
     3241 cagcgcgagg gcaagaccct cgtcggcgcc gagagccggt tcaccctcga ggtcgataac
     3301 gcgaagctca gcgggtcgat cgaccgcgtc gagatctcac cgacgggcga ggtcgtcatc
     3361 gttgacctga agaccggcac gccgatcacc acccagtcga ccatcgatga gcatccgcag
     3421 ctcgcggcat accagctcgc ctacaccgaa ggcatcctcg acgagccgct cgctggccac
     3481 gacgggcacc gacagggcgg agccaagctg ctcttcgtca agcagggcat cggctcgaag
     3541 ctctaccgcg aggcccatca ggctccgctc gacaccgatc agctggaggc cttccgcgag
     3601 cgggtgcgtg cggcggcgtc gctgatcgcg gcggccgagt tcgcgggtga ggcggagctt
     3661 ccgatctacg gtcggggaga cccttcgcgg ttgaggctgc agcgggtgcg ggcggtgtcc
     3721 agtgactgag ccgacgatca acgccgacca gatcgcggat gccctgtcgc gcccacgacc
     3781 gaccgcccag cagcgcgccg tcatcgagtc acctctcgcc ccagcgctcg tcgtggcggg
     3841 cgccggcagc ggcaagaccg agacgatggc ggggcgggtg ctgtggctgc tcgccaaccg
     3901 catggtcgac ccgagccaga ttctcggtct caccttcacg cgcaaggcgg cgggcgaact
     3961 cgccctgcgc atccgcgagc gtatcgccga cctcgcgcgg gtgggcctgc tcggcgaggg
     4021 ctacgacccg ttcgacgctc ccaccgtggc cacctacaac tcctacgcga acaccctcta
     4081 ccgcgacaat gccgcagccc tcggccggga gagcgacggt gccgttctcg gcgaggcggc
     4141 tgcttggcag ttggcgcgca cgaccgtgat cagaaccaag gatgaccggc tcgccgacat
     4201 cggcaagaac ctcgaccccg tcaccaaggc cacgctcacg atctcgcgct ccctcgcgga
     4261 gaacgtcgcg gtgcccgtcg aggtgcagga gttcgctcgg cgtttcgcgc gaatcgtcga
     4321 cctgcccccg ggcggtcgcg gtgcctacgc ccaggtcgac gggtgggccg agaccgtcgg
     4381 cgccctcgat gtgcttgtcg acctggccgc cgagtacggc gacgcgaagg tgcggcgcgg
     4441 gctggtcgag tactccgacc aggtggctct cgccctcgag attctgcgcc gccagccgcg
     4501 agtcgccgag atcgagcggg accggttccg tgtcgtgctg ctcgacgagt accaggacac
     4561 ctcggtcgtg caggcccggt tgctcgccca actgttcgcc gggcatccgg tgatggcggt
     4621 cggcgacccc aaccagtcga tctacggctg gcgcggggcg agcgcctcga acctcgacga
     4681 cttcgcgacc gcgttcgggg cgaccgacac gcgcttcgcg ctgtcgacga gctggcgtaa
     4741 cggcaggcgc atcctcgacg tcgccaacac gatcgtggcg ccgttcgtcg ccggcgcgaa
     4801 ggtgcaggtc gatcgtctcg acgcgagccc gaccgcgagc gacctgccgg tcgacatcga
     4861 gttcctcgag acggtcgccg acgaggcgga tgccgcggcc cgctggctca aggccaggct
     4921 cgacgtgcgc ggcacgccac cctccgcggc gatgctcttc cgcactcgca agacccaggg
     4981 cttcttcctc gaggcgatgc ggcggcacgg cgtgaagttc cacgtgctcg gcatcggagg
     5041 acttctcgcc gagcccgagg tcgccgacct ggtgtgcgct ctcaccgtga tgaacgatcc
     5101 gtcggcgggc ctcgagctgg ttcgcctgct cgcgggttcg cgctggcgag tcggggtgca
     5161 ggatctctgg gcactgcacc gtctcgcgtc atggctgcgg gatcgcgact atgcgcagca
     5221 gcgctacgac gacgaactcg cgcaggcact gaaggcctcg gtcgcggagg gcgagggcgg
     5281 ttcgatcgtg gatgctctcg acttcctgtc gaccgcgcgc gagggccaca ccgcgctcgc
     5341 tagcttcacg cccgaaggac tcgatcggct gcgtgacgca ggcaggctca tcgcgcgact
     5401 gcgctcgcgg ggtggcctcg atctgcccga cctcgtcgcc ttcgtgcttc aggagctcca
     5461 gctcgacatc gaggtctcgg cgaacgacta ccggccgctc ggagcatcca ccttcgaggc
     5521 gttcttcgat gcggtcggca actacctcgc gctcgaagag gtagcgacgc tcggcggctt
     5581 cctgtcctgg ttgcgcgagg ccgagcagcg ggaggatctc tccccgcgcc ccgaggatcc
     5641 ggagcccggg actgtgcagg tgctgacgat tcacggcgcc aagggtctcg agtgggatca
     5701 cgtggtcgtg ccgcgcctcg tcgacgacga gcttcccggt cggcccgtcg agggcggcta
     5761 cctcggctgg ctcgccttcg gtcaactgcc gtggtccttc cgcggggatg ccgccgagtt
     5821 gcccgtcttc gcctgggagt cccagaccac ccgcaaggat ctggtcgacg agcagaaggc
     5881 gttcggcggt cgggtgcgcg agcacctgct ggccgaggag cggcggctcg cctacgtcgc
     5941 ggtcacccga gcccggcacg ggctgctgct gagcggttcg ttctgggcga cccaggccaa
     6001 gccacgggtg ccgagcgtct acctcaccga gctgggcctc gacctgcccg aggggcctgc
     6061 gtccgatgag aacccgatgg gcgacgatct cgagcgcatc atctggccga tggacccact
     6121 cggtaggcgt cgtgcagcgg tcgagagcgc cgcagccgcc gtgcgcgaca cggatccggc
     6181 catcgtcgga ccgtggactg acgacgtcga gctgcttctc gaggagcgtc gccgtcggct
     6241 cggtgccccc gaacccgtcg aggttcccac ccgcgtgccg gcttcccgat tcaaggacta
     6301 cgtg
//
LOCUS       DAOSOU010000116         6147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12874_length_6147_cov_6.338148, whole genome shotgun sequence.
ACCESSION   DAOSOU010000116 DAOSOU010000000
VERSION     DAOSOU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12874_length_6147_cov_6.338148"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /gene="glgB"
                     /locus_tag="PLW15_07320"
     CDS             <1..1767
                     /gene="glgB"
                     /locus_tag="PLW15_07320"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOT34238.1"
                     /translation="VAGTSFSVWAPHARAVRVVGDFNGWNGVGHAMRRLDDNGVWEIF
                     LPALEPGSAYKFELLTQAGEWVTRADPMARYTEVPPATASKVGQSLFAWSDGDWMTRR
                     ANTDPHNSPMSVYELHVGSWRPGLDYRSLADELIDYVRQLGFTHVEFMPLAEHPYGPS
                     WGYQVTGYYAPTSRFGHGDDLKYLIDRLHGAGIGVIMDWVPGHFPKDEWALGKFDGQA
                     LYEHSDPRRGEQMDWGTLIFDFGQSQVRNFLVANALYWLEEFHIDGLRVDAVASMLYL
                     DYSRKDGEWLPNVNGGRENLEAISFLQEVNATAYKRNPGIVMIAEESTSWPGVTKPTA
                     SSGLGFGLKWNMGWMHDSLQYMQEDPMYRSYHHNDITFSFFYAFSENFLLPISHDEVV
                     HGKGSLLHKMPGDQWQKLANVRAYLSFMWAHPGKQLLFMGSEFGQPSEWSEERGLDWW
                     ILDQPLHLGLQKLVSELNRVYSGTAALWEQDNSPAGFEWIDGGNSAQNVVAFVRWSTS
                     GVPLVGVFNFSGNPVGPYRLGMPFGGVWNEIINTDAVEFGGSGVGNFGAVTALDEPWG
                     GRPASVELTLPPLAGLWLAPRG"
     gene            complement(1854..2285)
                     /locus_tag="PLW15_07325"
     CDS             complement(1854..2285)
                     /locus_tag="PLW15_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34239.1"
                     /translation="MTEQQLHRPTAGQLDVWSRVAGLGIVIGLLIGLVPPLAFGLTGP
                     GAAIILGILVVGPAIVSTVIGFRAVGVAKLEKASGYSTMFDFAGYELRDPRTLEVLRA
                     SDVRPTGTIRRSLFRSMLTVKPGTLLAKRLEEDERADRDER"
     gene            complement(2311..3222)
                     /locus_tag="PLW15_07330"
     CDS             complement(2311..3222)
                     /locus_tag="PLW15_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT34240.1"
                     /translation="MSNAPLNGANLRGAVDLSSLVRPPAPPAGGAPASAGGVVQQVTD
                     ATFTAVLDLSASVPVIVEFFGAGIQPTLGPVVRQYGGRLALATVDGTSNPQLVQAFQV
                     QEVPTVAAVIGGRPLQLFSGMMPEAELRTVLDQVLALAAQNGVTGTIPTDGDDEADPA
                     EPVEEPLPPLHQEAYDAITSGDFAAAIKAYETAIAQNPRDQLAVAGLAQVSLLARLEP
                     VTADAVRSAAAANPTDVDAQLAVADLDVSGGHLDDAFARLLDLFPTLDAAGKNAVRTR
                     LLEFFEIAGADDPRVIVARRRLTGLLY"
     gene            complement(3222..>3452)
                     /locus_tag="PLW15_07335"
     CDS             complement(3222..>3452)
                     /locus_tag="PLW15_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34241.1"
                     /translation="IVAVNLNEITTVQPVEAGAAVNPTGQVKALSGLAISTKGIRATY
                     GDQLLFYVPAAGSDAKIVLLGYSVGLVKAGEI"
     assembly_gap    3453..3552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3553..3938)
                     /locus_tag="PLW15_07340"
     CDS             complement(<3553..3938)
                     /locus_tag="PLW15_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34242.1"
                     /translation="MDSQLSPPAATVRQIEKIVARISSVAKKADADRNGELLATRFDG
                     AALELRLANYTIRGADPSIGALPAIPEGPVKLTLPQQTDTWPRTVFAVIQDEKDDTIP
                     PVALFLVQEDPRSDYKVSYAITLEPSA"
     gene            complement(3996..4754)
                     /locus_tag="PLW15_07345"
     CDS             complement(3996..4754)
                     /locus_tag="PLW15_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34243.1"
                     /translation="MRFVLAIVSFVVAAVMIVAGIAQRTIFAEPDKVALSTVVTTESP
                     VTVIDGDTLNAFEGSQTLSVQGPGTVFAAYGRTTDVLAWIGDASYTTVSLDKETGELT
                     STLTRGTETEVPDPNGSDLWLDDFVKDNDLSMTVKVPSNVSIIIASDGTEPAPVDIAL
                     SWPLDNSTPWAGPLIVGGAIVLLLGLGFLLWAINHMRSSRGPRRKMPKVPRQPIYKPT
                     KKAAVDKPASGRRRGMIAVPIVLVGALALSGCSP"
     gene            4997..>6147
                     /locus_tag="PLW15_07350"
     CDS             4997..>6147
                     /locus_tag="PLW15_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34244.1"
                     /translation="MAADDTDFGTEMRGYKKDEVDRAIQQLRGELIKANTDRAESAKE
                     VKRLLAVNEDLQAELDETGSPTYAGLGTKLENMLRVAEEQSTRLIAQADIDAEKLRAS
                     ALAETQELRSEAASAAQKLTDDANSRAAKLVADATQEANELLTRARADAQLTLQDAQR
                     EAAALRAAVATEAAEARSTAAREAAALRAAAEHEAAELKVTATREAAEASEAAAQLAR
                     DSELGRANYENENARLRAELARDIEQGRTDLERELETGRAQLEAEITAARETLANETA
                     AAQEKLANDTAAAQEKLANDTEASRAKLQADTAAAWGALNAETASTRAALEAETAAAR
                     AELEAETTAARNDLAAQTAAAQAALNADIADKRAALNAEIAEKRAALEN"
BASE COUNT         1068 a         2039 c         2041 g          899 t
ORIGIN      
        1 gttgcgggca cgagtttctc ggtgtgggcc ccacacgccc gcgcggtgcg ggtcgtcggc
       61 gacttcaacg gctggaacgg cgtcggccac gcgatgcgcc gcctcgacga caacggggtc
      121 tgggagatct tcctgcccgc gctcgaaccc ggcagcgcct acaagttcga actgctgacc
      181 caggccggcg agtgggtcac gcgagccgac ccgatggcgc gctacaccga ggtgcctccg
      241 gcgacggcat ccaaggtggg acagtccctg ttcgcctgga gcgacggcga ctggatgacg
      301 cggcgcgcca acaccgaccc gcacaactcg cccatgagcg tctacgagct tcacgtgggc
      361 tcgtggcgtc ccggcctcga ctaccggtct ctcgccgacg agctcatcga ctacgtacgt
      421 cagctgggat tcacgcacgt cgagttcatg ccgctcgccg agcatccgta cggtccgtcg
      481 tggggctacc aggtcaccgg ctactacgcg ccgaccagca ggttcggcca cggcgacgac
      541 ctcaagtacc tcatcgaccg gctgcacggc gccggcatcg gcgtgatcat ggattgggtg
      601 ccggggcact tccccaaaga cgagtgggca ctcggcaagt tcgacggcca ggcgctctat
      661 gagcacagcg acccgcgtcg cggggagcag atggactggg gcacgctcat cttcgacttc
      721 ggccagtcgc aggtgcgcaa cttcctggtc gccaacgcgc tgtactggct cgaggagttc
      781 cacatcgacg ggctgcgcgt cgacgccgtc gcgagcatgc tctacctcga ctactcccgc
      841 aaagacggcg agtggctacc caacgtcaac ggcgggcgcg agaacctgga ggcgatcagc
      901 ttcctgcagg aggtcaacgc gaccgcctac aagcgcaatc ccggcatcgt gatgatcgcc
      961 gaggagtcga cgagctggcc gggcgtgacc aagccgaccg cgtcatccgg tctcggtttc
     1021 ggtctgaaat ggaacatggg gtggatgcac gactccctgc agtacatgca ggaagacccg
     1081 atgtaccgct cgtatcacca caacgacatc acgttctcgt tcttctacgc gttcagcgag
     1141 aacttcctgc tgccgatcag ccatgacgag gtcgtgcacg gcaaggggtc gctgctgcac
     1201 aagatgcccg gcgaccagtg gcagaagctc gccaacgtgc gcgcgtacct gtcgttcatg
     1261 tgggcgcacc ccggcaagca gttgctgttc atgggcagcg agttcggtca gcccagcgag
     1321 tggagcgagg agcgcggact cgactggtgg attctcgacc agccgctgca cctcgggctg
     1381 cagaagctgg tgagcgagct caaccgggtc tactcgggca ccgcagccct ctgggaacag
     1441 gacaactcgc cggccggatt cgagtggatc gatggcggca actcggcaca gaacgtggtc
     1501 gccttcgtgc gctggtcgac gtcgggcgtg ccgctggtgg gcgtgttcaa cttctccggc
     1561 aaccccgtcg ggccgtatcg gctcggcatg cccttcggcg gcgtctggaa cgagatcatc
     1621 aacaccgacg ccgtcgagtt cggcgggtcg ggtgtcggca acttcggtgc cgtgacggca
     1681 ctggatgagc cgtggggcgg gcgtcccgca tcggtcgagt tgacgctgcc tccgctggcc
     1741 ggcctgtggc tggcaccgcg gggctagagc ttcggtgatc gagcttcggt gatcgagctt
     1801 gtcgagatcg tctcgcgcgc cggatctcga caggctcgat caccgctcac cgttcaccgc
     1861 tcgtcgcggt cagccctctc gtcctcctcg agccgcttcg cgagcagggt gcccggcttg
     1921 accgtgagca tcgaacggaa cagcgaccga cgaatcgttc ccgtgggccg cacatcgctt
     1981 gcccgcagca cctcgagcgt gcgcgggtcg cgcagctcgt agcccgcgaa gtcgaacatc
     2041 gtcgaatagc ccgacgcctt ctccagcttc gcgacgccga ccgcccggaa gccgatcacc
     2101 gtcgacacga tcgcgggacc gaccacgagg atcccgagga tgatcgccgc gcccggaccc
     2161 gtcaacccga aggccagggg cggcaccagc ccgatgagga gtccgatgac gatgcccagg
     2221 cccgcaaccc ggctccagac atcgagttga ccggcagtcg gtcggtgcag ctgctgctcg
     2281 gtcatcgttg actcctcgtg gtagccgcga tcagtacagc agaccggtga ggcggcgacg
     2341 cgccacgatg acccggggat cgtccgcacc ggcgatctcg aagaactcga gcagacgggt
     2401 gcgcaccgca ttcttgcccg ccgcgtccag cgtggggaac aggtcgagca ggcgcgcgaa
     2461 ggcgtcgtcg aggtgtccgc cgctgacgtc gaggtcggcc acggcgagtt gagcgtcgac
     2521 atcggtggga ttcgctgctg cggctgagcg cacggcatcc gcggtcaccg gctcgagacg
     2581 ggccagcagg gagacctggg cgaggccggc caccgcgagc tggtcgcggg gattctgcgc
     2641 tatcgctgtc tcgtaggcct tgatcgctgc cgcgaagtcg cctgacgtga tggcgtcgta
     2701 cgcctcctgg tgcaggggcg gcagcggctc ctccaccggt tcggccgggt cggcctcgtc
     2761 gtcgccgtcg gtcgggatgg tccctgtgac gccgttctgc gcggcaagtg cgagcacctg
     2821 gtcgagcaca gtgcgcaact ccgcttcggg catcatgccg ctgaacaact gcagcggacg
     2881 acccccgatc acagcggcaa cggtcggtac ctcctgcacc tggaacgcct gcaccaactg
     2941 ggggttgctc gtgccatcga ctgtcgcgag cgcgagccga ccgccgtact ggcgcaccac
     3001 gggacccagg gtgggttgga tgcccgcccc gaagaactcc acgatgacgg gcaccgacgc
     3061 cgacaggtcg agcactgccg tgaacgtcgc atccgtgacc tgctgaacaa cgccgccagc
     3121 ggacgccggc gctccccctg ccggcggtgc cggcggtcgc acgagcgaac tcaggtcgac
     3181 agcaccacga aggttcgcac cattcagggg tgcgttgctc attagatctc cccggccttg
     3241 acgagcccga cgctgtagcc gagcagaaca atcttggcat cgcttccggc cgcgggcacg
     3301 tagaacagca gttgatcgcc gtaggtcgcc cggatgccct tggtgctgat cgcgaggccg
     3361 gagagcgcct tcacctgacc ggtcggattg accgcggcgc cggcctcgac cggctgcacc
     3421 gtcgtgatct cgttgaggtt gaccgcgacg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nngctgacgg ttccagggtg atggcgtagc tgaccttgta gtcggagcgc
     3601 ggatcctcct gaacgaggaa cagggcaacc ggcggaatcg tgtcgtcctt ctcatcctga
     3661 atgacagcga acaccgttcg cggccaggtg tcggtctgct gcggaagggt gagcttcacc
     3721 gggccctcgg ggatcgcggg caacgcgccg atcgacgggt cggcaccacg aatcgtgtag
     3781 ttggcgaggc gaagctcgag agcggcgcca tcgaagcggg tggcaagcaa ctcgccattc
     3841 cggtcggcat ctgccttctt ggcgacggaa ctgatgcgcg cgacgatctt ctcgatctgg
     3901 cgcaccgtgg ccgccggcgg gctgagctgg gagtccacgg gggcctccga cgccgacgcc
     3961 gagggcgcgg ccgccttctg gtcggtcggc cagaactagg gcgagcatcc gctcagcgcg
     4021 agggcaccga cgagaacgat gggcacggcg atcatgcccc ggcgacgccc gctcgcgggc
     4081 ttgtcgactg ccgccttctt cgtgggcttg tagatcggct ggcggggcac cttgggcatc
     4141 ttgcgacgcg gcccgcgcga cgagcgcatg tggttgatcg cccagagcag gaagccgaga
     4201 ccgagaagca gcacgatcgc tccgccgacg atcagcggtc ccgcccacgg ggtcgagttg
     4261 tcgagtggcc acgacagggc gatgtcgacg ggcgcaggtt ccgtaccgtc ggaggcgatg
     4321 atgatcgaca cgttcgacgg aaccttgacc gtcatggaga ggtcgttgtc tttgacgaag
     4381 tcgtcgagcc acaggtcgga accattcggg tccggcacct cggtctcggt tccccgggtc
     4441 agcgtcgacg tcagctcacc ggtctccttg tcgaggctga cggtggtgta gctggcgtcg
     4501 ccgatccacg ccagcacatc ggtcgtgcga ccgtatgcgg cgaacaccgt gccgggcccc
     4561 tgcaccgaga gagtctgact gccctcgaac gcgttgaggg tgtcaccgtc gatcacggtc
     4621 accggagact cggtcgtgac cactgtgctg agcgcgacct tgtcgggctc ggcgaagatc
     4681 gtgcgctggg cgatgcctgc gacgatcatg accgcggcca cgacaaaact gactattgcg
     4741 agaacaaacc gcacgaacta ctcctttcgt gttgcccggc cggctcgtta cgagctggca
     4801 ccctccgccg ctgggtagtc ggtcgctcgc actagggacg agaggagggg atccccttca
     4861 ctagcgggga atggcaacaa caaagacgtt caagaatagc cgacgggcct gcgtcgggcc
     4921 taaccacagc actgggaatg cactgggaat gaccacgcta aacttctgac agtcccccac
     4981 cgcgcaagga gaaatcatgg ctgccgacga caccgatttc gggactgaga tgcgcgggta
     5041 caagaaggac gaggtcgacc gcgccatcca gcaactgcgt ggcgagctca tcaaggccaa
     5101 caccgatcgc gccgagtcgg ccaaggaagt caagcgcctg ctggccgtca acgaagacct
     5161 gcaggccgag ctcgacgaga ccggcagccc cacctatgcc ggactgggaa ccaagctcga
     5221 gaacatgctg cgcgtggccg aggagcagtc cacccgcctc atcgcgcagg ccgacatcga
     5281 cgccgagaag ctgcgcgcga gcgccctggc cgagacccag gagttgcgat ccgaggctgc
     5341 cagcgccgcc cagaagctga cggatgacgc gaactcccgc gccgcgaagc tcgtcgccga
     5401 tgccacgcag gaggccaacg agctgctcac ccgcgcccgc gctgacgcgc agctcaccct
     5461 gcaggatgct cagcgtgagg ctgccgcgct ccgcgctgcc gtggccaccg aggccgccga
     5521 ggcccgctcc accgccgctc gtgaggccgc agcactccgc gcggcggccg agcacgaggc
     5581 agccgaactc aaggtcacgg ccacccgtga ggcggcggag gcgagcgagg ccgcggcgca
     5641 gctggcccgg gactccgagc tgggtcgggc caactacgag aacgagaacg cgcgactgcg
     5701 cgccgagctc gcccgcgaca tcgagcaggg cagaaccgac ctggagcgcg agctcgaaac
     5761 cggtcgcgcc cagctggaag ccgagatcac cgccgcgcgc gagactctcg ccaacgagac
     5821 cgcggccgcg caggagaagc tcgccaacga cactgcggca gcgcaggaga agctcgccaa
     5881 cgacacggag gcatcccgcg cgaagcttca ggccgacacg gccgcagcct ggggcgcact
     5941 caacgctgag acggcatcga cccgcgcagc cctcgaggcc gagactgcgg ccgcgcgagc
     6001 cgaactggag gcggagacga ccgccgcgcg caacgacctg gcggcgcaga ccgccgccgc
     6061 acaggcggcg ctcaacgccg acatcgccga caagcgcgca gcactcaacg cggagatcgc
     6121 ggaaaagcgc gcagccctcg agaacga
//
LOCUS       DAOSOU010000117         6138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12905_length_6138_cov_6.183627, whole genome shotgun sequence.
ACCESSION   DAOSOU010000117 DAOSOU010000000
VERSION     DAOSOU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12905_length_6138_cov_6.183627"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2300
                     /gene="glgP"
                     /locus_tag="PLW15_07355"
     CDS             <1..2300
                     /gene="glgP"
                     /locus_tag="PLW15_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOT34245.1"
                     /translation="LHAYLTEPRWYQTLTDAPASIGYFSPEFGIAAALPQYSGGLGIL
                     AGDHLKSASDLGVPIIGVGLFYRAGYFRQAISREGWQLESYPVLDPDGLPLTVLRHPD
                     GTAAQVALALPDGRALYARVWQVAVGRIRLLLLDTDIPENADELRSVTDRLYGGGGEH
                     RLLQELLLGIGGVRAIKAYTELTSTPFPEVFHTNEGHAGFLGLERISDLIGAGLSFAT
                     ALQVVRAGTVFTTHTPVPAGIDRFEVDMITKYFSTDLLPGVAASDVIALGEENYEGGD
                     ATKFNMAVMGLRLAQRANGVSKLHGEVSREMFGALWPGFEPEDVPITSVTNGVHAPTW
                     TDPMLAALAAEKLGTGDTTGCDWNSKSVTDADLWAVRGRMREQLVTDARRRVSAAWQE
                     QNPGVGAPAWYSELLDPTVLTIGFARRVPTYKRLTLMLHDEARLRSLLLHPERPIQIV
                     IAGKSHPADDEGKRLIQRLVEFASEPEVRKRIVFLPNYDIGMAKLLYPGTDVWLNNPL
                     RPLEACGTSGMKAALNGSLNLSILDGWWAEFYDEENGWAIPSADSAGDGAERDKLEAD
                     SMYDLIEHQIAPRFYDRGKNGVPTRWVESIRHTLATLSPALSADRMVKEYVERLYMPA
                     AAAEKVVSADGFRPARELADWKARVTAAWPKVHVSHVESGGVDAVPQVGDDLRLRATV
                     DIDGLAPTDVAAEVVYGRARDSDRIDDVVHQGLELASGEERPVVFAGTVPLARAGSFG
                     YNVRVVPSHPLLASAAEMGLIAVAE"
     gene            3126..3386
                     /locus_tag="PLW15_07360"
     CDS             3126..3386
                     /locus_tag="PLW15_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOT34246.1"
                     /translation="MQVVAAPGIRVDLLIDGWLVIELDGSRFHSGPAEFEADRARDAV
                     LATWAYRTLHFSYRQVMDDWDFVYSTILAVRSQGAPAGRRPV"
     gene            complement(3510..4004)
                     /gene="ybaK"
                     /locus_tag="PLW15_07365"
     CDS             complement(3510..4004)
                     /gene="ybaK"
                     /locus_tag="PLW15_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOT34247.1"
                     /translation="MPKSHRPDAPGTPATVALAAAGIRFTAHSYDHDPATTNFGLEAA
                     SALELDPEQVFKTLLADVDGALVVGIVPVTGKLDLKALAAAVGGKKAEMADPKLAERR
                     TGYVVGGISPIGQKNAHPTVLDETAELYDTIFVSGGRRGLDIEIAPADLVRATGAVIA
                     AIAR"
     gene            complement(4019..6060)
                     /gene="glgX"
                     /locus_tag="PLW15_07370"
                     /pseudo
     CDS             complement(4019..6060)
                     /gene="glgX"
                     /locus_tag="PLW15_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
BASE COUNT          893 a         2028 c         2171 g         1046 t
ORIGIN      
        1 acctccacgc ctacctgacc gagccgcgct ggtaccagac cttgacggat gcgccggcct
       61 ccatcggcta cttctcgccc gagttcggaa tcgccgccgc cctgcctcag tactccggcg
      121 gcttagggat cctcgcggga gaccacctca agagcgcgag cgacctcggc gttcccatca
      181 tcggtgtcgg cctgttctac cgggccggct acttccgcca ggcgatctcg cgcgagggct
      241 ggcagctgga gagctacccg gtgctcgacc ccgacgggct gccgctcacc gttctgcgtc
      301 atcccgacgg caccgcggcg caggtcgccc tcgcgctgcc cgacggccgc gccctctacg
      361 cgcgcgtgtg gcaggttgct gtgggtcgca tccggctgct gcttctggac acggacatcc
      421 cggagaacgc cgacgagctg cgcagcgtga ccgaccgcct ctacggtggc ggcggcgagc
      481 accgactgtt gcaggaactg ctgctcggca tcggcggcgt tcgggcgatc aaggcgtaca
      541 ccgagctcac gagcacgccc ttcccggagg tgttccacac caacgagggt cacgcgggat
      601 tcctcgggct ggagcgcatc agcgacctca tcggggcggg gctgtcgttc gcgaccgcgc
      661 tccaggttgt gcgcgccggc accgtcttca ccacgcacac gcccgtgccg gccggaatcg
      721 accggttcga ggtcgacatg atcacgaagt acttctcgac cgacctgctg ccgggggttg
      781 cggcatccga cgtcatcgca ctcggcgagg agaactacga gggcggcgac gcgaccaagt
      841 tcaacatggc cgtcatgggc ctgcgactcg cgcagcgcgc caacggtgtc tcgaagctgc
      901 acggcgaggt gtcgcgcgag atgttcggtg cgctctggcc ggggttcgag ccggaggacg
      961 tgccgatcac gtcggtgacc aacggggtgc acgcgccgac ctggaccgac ccgatgctcg
     1021 cggcgctcgc cgccgagaag ctcggcaccg gcgacaccac cggctgcgac tggaactcga
     1081 agtcggtgac cgacgccgac ctctgggcgg tacgcggtcg catgcgcgag caactggtga
     1141 cggatgcccg ccgccgggtc agcgcggcgt ggcaggaaca gaaccccggc gtcggcgccc
     1201 ccgcctggta ctcggagctg ctggacccga cggtgctgac gatcggcttc gctcgacgcg
     1261 ttcccaccta caagcggctg accctcatgc tccacgacga ggcgcggctg cggtcgctgc
     1321 tgctgcaccc cgagcggccg atccagatcg tgatcgccgg caagtcgcat ccggccgacg
     1381 acgagggtaa gcggctcatt cagcggctcg tcgagttcgc cagcgagccg gaggtgcgca
     1441 agcgcatcgt cttcctgccc aactacgaca tcggcatggc gaagctgctc tacccgggca
     1501 ccgacgtctg gctgaacaac ccgctgcgtc cgctcgaggc gtgcggaacc tcgggcatga
     1561 aggccgcgct caacggctcg ctcaacctgt cgatcctcga cggctggtgg gcggagttct
     1621 acgacgagga gaacggttgg gccatcccgt cggccgactc ggctggtgac ggcgccgagc
     1681 gcgacaagct cgaggccgac agcatgtacg acctcatcga gcaccagatc gcaccgcggt
     1741 tctacgaccg cggaaagaac ggcgtgccca cccgctgggt ggagtcgatc cggcacacgt
     1801 tggcgaccct gtcgccggcg ctctccgccg accgcatggt caaggagtac gtcgagcggt
     1861 tgtacatgcc ggcggccgcg gccgagaagg tcgtgagcgc cgacgggttc cgcccggccc
     1921 gcgagctggc cgactggaag gcgcgcgtga ccgccgcctg gccgaaggtg cacgtgtcgc
     1981 acgtcgagtc cgggggagtg gatgccgtgc ctcaggtcgg cgacgacctg cggctgcgcg
     2041 cgacagtcga catcgacggg ctcgccccca ccgacgtcgc cgctgaggtc gtgtacggtc
     2101 gcgctcgcga cagcgaccgc atcgacgacg tcgtgcatca gggtctcgag ttggccagtg
     2161 gcgaggagcg tccggtggtg ttcgcgggca cggtcccgtt ggcgcgagcc gggagcttcg
     2221 gctacaacgt gcgggttgtg ccgtcgcatc cgctgctcgc cagcgccgcg gagatgggcc
     2281 tgatcgccgt cgctgagtag cccgcagcgt ctggcttcct gttcgccgat caggagatct
     2341 tggcgacacg ccgagtgggg ttggtgagtc gcggcgcgtc gcgaagatct cctgatccgc
     2401 gaggtaacga ctgatcccta acagggtccc cgggacgcca cggcactctg aaacgggatg
     2461 ccccgccccg accagcccgt ccgcggagcc atgctgtgcc gatgcccggt ctcgcagcgg
     2521 tattcctcag cggtcgggga tgtgcgcgtc gctccgagct ggtggaggcc gggttcacac
     2581 cgcacgcgat ctcctccatg gtgcgatcgg gtgagatcga gcgcgtgcgg atcggctggt
     2641 acgttccgcg aggtgctgac ccgatgctgc gccaggcggt gcgtgtgggc ggccgcttgg
     2701 cctgcgtgtc ggctgcaggt cacatcggat tgtgggtgcc cgagggaagc gccttgcacg
     2761 tggccgtggc gcgcaatgca tccaggtttc gatcaccgtc tgcccatccc ctgccactct
     2821 cacagagcgc agccagtgcg ggggtcgatc tccattggct ttccaccccg ggcacgcggt
     2881 gcgaaggggt cagctctctg cgggagtgcc tcgcggaggt cgtagactgc cagcccgtcg
     2941 agctggcggt cgcggttgtc gactcggcgc tgaatctggc ggccatcacg actgctggtc
     3001 tgcacgagtt gctgtcctcc actacccgtg gtcgtgccgt tctcgggctc gttgatggcc
     3061 gtgctgagag tggcatcgag tcgattgcac gggtgcgctt gcggcgggcg ggtatccggg
     3121 cgcacgtgca agtggtagcg gccccgggca tccgcgtcga ccttctgatc gacggctggc
     3181 tcgtgattga gctcgacggc agccgatttc actcgggccc agccgagttc gaagctgatc
     3241 gtgcccggga tgccgtgctc gccacgtggg cgtaccgcac gctccacttc tcctaccgtc
     3301 aggtgatgga cgactgggac ttcgtctact cgacgatctt ggccgttcgg tctcagggtg
     3361 cgcccgcggg tcgccggccg gtgtgatccc cgtgcggggg ttcgcggatc aggatgccta
     3421 cgcgacacgc cgggctcagt tgcgtcgccc acggcgtgtc gcggcgcgca tcctgatccg
     3481 cgaagatggg cggcaggggc cgccgcgcct cagcgtgcga tcgcggcgat caccgcgccg
     3541 gtcgcgcgca ccaggtcggc gggggcgatc tcgatgtcga gtccgcggcg cccgccactg
     3601 acgaagatcg tgtcgtacag ctcggcggtc tcgtcgagca cggtcggatg cgcgttcttc
     3661 tgcccgatcg gactgatgcc gcccaccacg tagcccgtgc gccgctcggc gagcttcggg
     3721 tcagccatct ccgccttctt gccgccgacc gccgccgcga gcgccttgag gtcgagcttg
     3781 ccggtgaccg gcacgatgcc cacgacgagt gcgccgtcga cgtcggcgag cagggtcttg
     3841 aacacctgct ccgggtcgag ctcgagcgcg ctcgcggcct caagtccgaa gttggtggtg
     3901 gccgggtcgt gatcgtagct gtgagccgtg aaccggatgc ccgccgcagc cagcgccacc
     3961 gtggcaggtg tgccgggggc atcgggacgg tgggatttcg gcatggtcat tcagtgagtc
     4021 agtgcgccac gaacagctgc atcgacgcac ccgacacggt cagctccgag ccgggcaggt
     4081 agtcgacgag cgggtcgaca tgctcctcct gggcggaatc ccacaacagc gagtagccgg
     4141 cgacgccctc gtgagcgggc agcgtcaccg tcacgtcatc ttcgaggccg tgcacgatga
     4201 gcaggatgcg gttgaagtcc tcgaactcgg gcgtcgacgc cgcgaggtac tgcagggtgc
     4261 gctccgcggg ggagtcccag tcctcctcgc tcatcgactc gccctgcttg ttgtaccagt
     4321 ccatctgggt ggcgctcgga acggtctcgc cccacttgcc gtagcgcacc ggacgcaacg
     4381 cggggttctc acgccggaac tggatgagca tgcgggtgac ggcaagcagg tcctcctgcc
     4441 actcctcgtg ctgccagggc agccaggtga gttccgagtc gtggcagtag gcgttgttgt
     4501 ttccgcgctg ggtgcgcccg aattcgtcgc ccgcggtcag catcgggatg cccgccgaca
     4561 gcaacagcgt gccgagcagg ttgcgcatgg ccttgcgtcg gagtcgacga tgtccttctt
     4621 ggtggtcgga ccctcgacac cgtgattgaa ggagtggttg ttgtcggtgc cgtctcggtt
     4681 gttctcgccg ttgccgaggt tgtgcttgcc gttgtaggcg gtgaggtcgg ccatggtgaa
     4741 gccgtcgtga gcggtcacga agttgaggct cgccatcggt ccgcgctcct gagagaacac
     4801 gtgggccgag ccggccagtc gccgcgccag cgagccgatg ccgttgggcg cggagccgtt
     4861 cgcgcgggcc gcaccgatgt cggtgagcca gaagtcgcgc atgcggtcgc ggtagccgtc
     4921 gttccactcg ctccagccgt cggggaactt gccgacctgc cagccgccca tgcccacgtc
     4981 ccacggctcg gcgatcatcg tgacggactc gagttgaggg tcgtcgagga tcgccagcag
     5041 aagcggatgc tcgggctgga actcgttcgc ggcatcccga cccagcgtcg cggcgaggtc
     5101 gaagcggaag ccgtccacct gcaggtcgtt cgcccagtac ctgagcgagt cgaggatgag
     5161 ccgctgggcg gcgggcgtcg cggtattcac ggagttgccg catccggtca catcgacgta
     5221 ggcaccgtcg ggggtctggc ggtagtagtt cttgttgtcg atgccgcgca ggctcgtggt
     5281 gggaccgccg aggccctcct ccgccgtgtg gttgtagacc acatcgagga tcacctcgag
     5341 cccggcctcg tgcagcagct tgaccatgcc cttgaactcg cgcagcacgg caccggtgcc
     5401 gccgaactgg gcctcgcgcg tcgcataggc ggcgtgcggg gtgaagaagt tgagggtgtt
     5461 gtagccccag tagttggtga gcccctgttt gatgaggcgc tgctcgctca cgaattggtg
     5521 gatcggcagg agctgcaccg tggtgacgcc gagatccttg aggtagccga tcgtggaggc
     5581 atggccgagg cccgcgtacg ttccgcgcaa ctcgtcgggc aggtcggggt tgagcttgga
     5641 gatgcccttc gcgtgagcct cgtagatcac ggcgtgatcc atgccgatgc gcggcttctt
     5701 cgaggtgccc cagtcgaagg tgtcgggctc ctgcacgtag ctgcgccact cgccgctgga
     5761 ggtgcgggcg agcccccgcg cgtaggggtc gagcaggtgc aggctcgggt cgaacgcgtg
     5821 cccggcagtc gacggaccgt tggcccggat cgagtaccgc gtgcccgggg cgagcagctt
     5881 cgaggtcacg gtccagacgt caccgtcggt cttggccagc ggcagggtgg tggcgaccca
     5941 gctcgcatcc ttgtcgtcga agatgcacag atccatcgcc gtcgcgctgg ccgaccagac
     6001 gcgcagcacg ccgccgtcgg gtgtgaggcg cacaccgagg tcggtgaggg aatcgggcat
     6061 gcgttctaga gtaatgaagg ctcggagagc ccgatgacca cggggaggca cgcgcgtgaa
     6121 cgtctatctc gaccacgc
//
LOCUS       DAOSOU010000118         6118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_12973_length_6118_cov_5.885370, whole genome shotgun sequence.
ACCESSION   DAOSOU010000118 DAOSOU010000000
VERSION     DAOSOU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12973_length_6118_cov_5.885370"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="zapE"
                     /locus_tag="PLW15_07375"
     CDS             <1..724
                     /gene="zapE"
                     /locus_tag="PLW15_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOT34248.1"
                     /translation="LLAALWHVAPGRKYFGTFIEYTALVGALGYAPTVKLLTGASLLC
                     IDEFELDDPGDTRLMSRLLGELVATGSRIAATSNTPPNALGEGRFAAQDFLREIDSLA
                     AKFTTIRIDGLDYRRRDTEAHAHSVDDVELGLAAFSGAVTLDSFDAALAHLATVHPSR
                     YVKLVDGLDAIGLTDVHVLHNQTDALRLVAFVDRLYDAQVRIVASGVPLDSIFPDDML
                     SGGYRKKYLRSVSRMIALTDPR"
     gene            complement(708..1868)
                     /locus_tag="PLW15_07380"
     CDS             complement(708..1868)
                     /locus_tag="PLW15_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOT34249.1"
                     /translation="MSLLRSERFSAILLVAAAAVGLLLANTAAAPAAKAVESFHFGIP
                     ALGLDLSVGHWIKDGLLAIFFFIAAIELRHELRHGELDSPRKAIVPTVAAIGGVVVPA
                     LIFLVIVRDPELSQGWPIPTATDIAFALGVLAIVGKGLPPTFRALLLALAVIDDLIAI
                     LIIAAFFTKDLAPIPLLIALPVVLLFGWLSYRVTKRRRAWVIPLMILLGIAAWVLVYL
                     SGIHPTIAGVALGLIMADATGGRSRHAIEPWSNVVILPLFAIVAALVPIPSTNLAGLS
                     PAFWAVIIALPFGKLVGITGGAAIATALARRSTGSTAHLPLPDIIAVAGLGGIGFTVS
                     LLMNELAFEGQGSVADEVTLAVIVASVIAALVGGVLTSLRSRHYARAATADR"
     gene            2080..3351
                     /locus_tag="PLW15_07385"
     CDS             2080..3351
                     /locus_tag="PLW15_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT34250.1"
                     /translation="MDQGNTAFLLLASALVLLMTPGLAFFYGGLVKAKSVISMMMMSF
                     GAIGLIAVLWAIYGYAIAFPTIDGLAPSPWMFDSSNFLLGAALEDAAAPAAGYPALAF
                     VAFQATFAIITVALISGAIADRAKFGSWMIFAGIWATVVYFPVASWVFNFGLADGAFT
                     YGGWLTRGLQDAFGTGLIDFAGGTAVHINAGAAALALALVLGRRVGFAKGAHVPHNPP
                     FVLLGAGLLWFGWFGFNAGSELAADGTAAIAFVNTLIAPAAALLGWLVVERIKDGKPT
                     SIGAASGAVAGLVAITPACGSLTPGWAIVLGALAGIICAVAIDLKFKLGFDDSLDVVG
                     VHLVGGLVGTLFLGFFANGTGLIYSGTFTQLLVQAIAALSVMIYSFVLAFIIGFVIEK
                     TIGFRIKNEDEIAGVDLVVHGEEGYVLADVK"
     gene            3446..4489
                     /locus_tag="PLW15_07390"
     CDS             3446..4489
                     /locus_tag="PLW15_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34251.1"
                     /translation="MGQGWGLAALSVIAIAVMIAAIMVVYSRMLPTGAPIGVSLACVA
                     VGTVTWLAVTIILRPALPAELVAPIAALGGLAALLATIVPRAFGAGAPGSVAFAAIWS
                     LLVFVPVAWLSFSGLGPFGVRPIDQGGSLAINVAAGAAAVGVLVSGGAGAPRLRPSAV
                     PRGFATGGVILLTLGWIGWLVGAELAIDDLTPGILINGAFAAGGGVVGWLVVQRIRHQ
                     STTLSAVAAGIVSGLVGVSAGAPMYGAPAAFVVGIIASASACLFTLRRVGDTRRQQWF
                     IVGSHLIAGAVGLVALGLFANDLGFLFTGQYVFVRDQVICCILATGWSMGVAALLWVV
                     LRRRGDRTPRSAS"
     gene            4486..5211
                     /locus_tag="PLW15_07395"
     CDS             4486..5211
                     /locus_tag="PLW15_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HOT34252.1"
                     /translation="MTAPEPIAVQPPALQGRVVASQRWTRLTFLHWRVESSRVAPLLP
                     AGVRPDEHDGSSWVGLIAFHLGDATIAGSPPVPYFGDFAEVNVRLYGVDASGRRGVVF
                     ASLEASRLAAVLAARAAFSIPYFWSATSLIETGGAIEYGARRHVGDGATRLVARPGAE
                     LVIGDATADFLTARWGLFTRRLGRTVFLPNTHEAWRLQRATLLELDDTLVERAGLPHV
                     TDRMPDSVLYAEGVTARFGRALR"
     gene            complement(5180..6001)
                     /locus_tag="PLW15_07400"
     CDS             complement(5180..6001)
                     /locus_tag="PLW15_07400"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOT34253.1"
                     /translation="MLREFATGVSLLGRGLGTWRTAPGLMLLGMVPALIVGAVILAGL
                     IALGVNIEAIAVAVTPFASEWPEPWYTAVHVVAGLAFLVVAILLVVYTFTAVTLIVGD
                     PFYQRIWRHTEGRFGTVPDEGASGFWRELGRGISSGLRMLVPAVLIGVMLFVLGFIPV
                     VGQVVVPVLGAFFGGWYLTVELTGLAFDSRGRSLAERRRALRGRRAMTLGFGIATWLV
                     FLIPGGAVIMMPAAVGGATLLARRVLGESTDQRTRTDQQTCTDPRATSGLDRTAP"
     gene            complement(6043..6118)
                     /locus_tag="PLW15_07405"
     tRNA            complement(6043..6118)
                     /locus_tag="PLW15_07405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6083..6085),aa:Val,seq:cac)
BASE COUNT          983 a         1993 c         2041 g         1101 t
ORIGIN      
        1 tctgctcgcg gcactgtggc acgttgctcc cgggcgcaag tacttcggca ccttcatcga
       61 gtacaccgcg ctcgtgggcg ccctcggtta tgccccgacc gtcaagctcc tcaccggcgc
      121 ctcgctcctg tgcattgacg agttcgagct ggatgacccc ggcgacaccc gcctgatgtc
      181 gcgtctgctg ggtgagctgg tcgcgaccgg ctcgcgcatt gccgcgacct ccaacacccc
      241 gcccaatgcg ctgggagagg gacggttcgc ggctcaggac ttcctgcgcg agatcgattc
      301 actcgcggcg aagttcacca ccatccgcat cgacgggctc gattaccggc gacgcgacac
      361 cgaggcgcac gcccacagcg tggatgacgt cgagctcggt ctcgccgcgt tcagtggagc
      421 cgtcacgctt gactcattcg acgctgcgct cgcccatctg gcgaccgtgc acccgtcgcg
      481 ctacgtgaag ctcgtggacg ggctcgatgc gatcggactg acggatgtgc acgtgctgca
      541 caaccagacc gacgcactgc gcctcgtggc tttcgtcgac cgactgtatg acgcgcaggt
      601 acgcatcgtc gccagcggcg ttccgctgga ttcgatcttc cccgatgaca tgctctccgg
      661 cggctatcgc aagaagtacc tgcggtctgt ctcccggatg atcgccctca ccgatccgcg
      721 gtagctgcgc gggcgtagtg ccgacttcgg agtgacgtca gcacgccgcc caccagcgca
      781 gcgatcaccg aggcgacaat cacggccagc gtcacctcat cggccacgga accctgtccc
      841 tcgaaggcga gctcgttcat cagcagcgaa acggtgaagc cgattccgcc caggcccgcg
      901 acagcgatga tgtccggcag cggcaggtgc gcggtggagc cggtgctgcg ccgcgccagt
      961 gccgtcgcga tcgcggcacc gccggtgatg ccgaccaact tgccgaaggg tagcgcgatg
     1021 atgaccgccc agaacgcggg actgagaccg gccagattcg tcgaggggat cggtaccagt
     1081 gccgcgacaa tggcgaacaa cgggaggatg acgacgttgg accagggttc gatggcgtgc
     1141 cggcttcggc cgcctgtggc atccgccatg atcaggccca gtgcaacccc cgcgatagtc
     1201 gggtggatgc ccgataggta cacgagcacc cacgcggcga tgccgaggag gatcatgagc
     1261 gggatgaccc atgcccgacg ccgcttggtc accctgtagc tcagccaacc gaacagcagc
     1321 acgacgggca gggcgatcag cagtgggatg ggtgcgaggt ccttcgtgaa gaaggcggcg
     1381 atgatgagga tcgcgatcag gtcgtcgatc accgcgaggg caagcaggag cgcccggaac
     1441 gtcgggggaa ggccctttcc gacgatcgcg agaacgccga gcgcgaaggc gatgtcggtc
     1501 gccgtcggga tcggccagcc ctgactcagt tccggatcgc gaacgatcac gaggaagatc
     1561 agggcgggaa ccaccacccc gccgatggct gcaaccgtgg gcactatggc cttgcgaggc
     1621 gagtccagct caccgtggcg cagttcgtgc ctcagctcga tcgccgcgat gaagaagaag
     1681 atcgcgagca gtccgtcttt gatccagtga ccgaccgaca gatcgagccc cagggccgga
     1741 atgccgaagt ggaacgactc gacggccttg gccgctggtg ccgccgcggt gttggccagc
     1801 agcagtccga ctgctgcggc agcgaccaaa aggatggccg agaatcgctc ggagcgcagc
     1861 aatgacatga agtcgagtgt agaagaaggc cccgcaggcg accggtggca cgacgaaaca
     1921 cgatgtttac gtggctgtgc ccggtgtaac agaaccgaaa cacgaccggc actcagatcg
     1981 aaacctcccc tcgacaggat gtgaccgtac ccgaggcgtg atcctccccg tgcggaccca
     2041 acggtgcatc cttcgcagaa ccaaagagag gtacacacaa tggatcaggg caacactgcc
     2101 ttccttctcc tggcatcagc gctcgtgctg ctgatgacgc cgggactggc attcttctac
     2161 ggcggcctcg tcaaggccaa gagcgtcatc agcatgatga tgatgagctt cggcgcaatc
     2221 ggcctcatcg cggtgctgtg ggcgatctat ggatacgcga tcgcgttccc gacgatcgac
     2281 ggcctcgcgc cgtcgccgtg gatgttcgac agcagcaact tcctcctcgg cgcggcgctg
     2341 gaggatgctg ccgctcccgc ggccggttac ccggccctcg cattcgtcgc gttccaagcg
     2401 accttcgcga tcatcactgt tgcgctgatc tcgggtgcta tcgctgaccg tgcgaagttc
     2461 ggatcgtgga tgatcttcgc cggaatctgg gcgacggtcg tctacttccc ggtcgcgagc
     2521 tgggtgttca acttcggtct cgccgatggc gccttcacct acggtggatg gctgactcgt
     2581 ggtctgcagg acgcgttcgg cacgggcctg atcgacttcg ccggtggcac cgcagtgcac
     2641 atcaacgctg gtgccgccgc tctcgccctc gccctcgttc tcggacgtcg tgtcggcttc
     2701 gccaagggcg cccacgttcc tcacaacccg cccttcgttc tgctgggtgc cggtcttctg
     2761 tggttcggtt ggtttggttt caacgccggt tcggaactgg cggctgacgg aaccgcggca
     2821 atcgccttcg tgaacaccct catcgccccc gctgccgctc tgctcggctg gctcgtggtc
     2881 gagagaatca aggacggcaa gcccacttcg atcggtgcag cgtcgggtgc cgttgcaggt
     2941 ctggttgcga tcacccccgc ctgtggatcg ctcacgcccg ggtgggccat cgtgctcggc
     3001 gctcttgccg gaatcatctg cgcggtcgcc atcgacctga agttcaagct cggtttcgac
     3061 gactcgctcg acgtcgtggg tgtccacctc gtcggtggcc tcgtgggaac gctgttcctg
     3121 ggcttcttcg ccaacggcac tggcctgatc tacagcggca ccttcaccca gttgctggtg
     3181 caggcgatcg ccgcgctcag cgtgatgatc tattcgttcg tgcttgcttt catcatcgga
     3241 ttcgtgatcg agaagaccat cggattccgc atcaagaacg aggacgagat cgccggtgtg
     3301 gacctggtcg tgcacggtga agagggctac gttctggctg acgtcaagta gtcattcgac
     3361 acactcaagc aaggggcgtc gctcgcagga gcggcgcccc ttactctgtt tgtcgagagc
     3421 caagctctcg gcgaaagggg aacacgtggg gcaagggtgg ggcctcgccg cgctgtcggt
     3481 gattgccatc gcggtgatga tcgcggcgat catggtcgtg tacagccgga tgctgcccac
     3541 gggcgctccg atcggtgtct cactggcgtg cgtcgcagtg ggcacggtga cctggctcgc
     3601 ggtgacgatc atcctgcgtc ccgcgcttcc cgcggagctt gtcgcgccga ttgccgctct
     3661 cggtggtctg gctgcgctgc ttgccacgat cgtgccgaga gcgttcggtg ctggtgcgcc
     3721 ggggtcggtt gcgttcgccg ctatctggag tctgctggtg ttcgttcccg tggcttggct
     3781 gtcgttctcc ggactcggac cgttcggtgt gcgaccgatc gatcagggcg ggtcgctcgc
     3841 gatcaatgtc gcggccggcg cagctgcggt tggcgtgctc gtgtccgggg gagcgggagc
     3901 tccccgactt cgaccgtcgg cggtgcctcg cggatttgcg accggaggtg tgatcctgct
     3961 gactctcggg tggatcgggt ggttggtggg tgcggagctc gctatcgacg acctcactcc
     4021 gggcatcctg atcaatgggg ccttcgcggc cggtggggga gtcgtgggct ggttggtggt
     4081 gcagcgcatt cgtcaccagt cgaccaccct gagcgccgtg gccgccggta tcgtcagcgg
     4141 tctggttggc gtcagcgccg gcgccccgat gtatggtgca cccgccgcgt tcgtcgtggg
     4201 gatcatcgcg tcggcttcgg cctgcctctt cacgctgcgg cgggtgggcg acactcgacg
     4261 ccagcagtgg ttcatcgtgg gctcgcacct gatcgctggt gcggtcgggc tagtggcgct
     4321 cggcttgttc gccaacgatc tgggcttcct gttcacgggc cagtacgtat tcgtgcggga
     4381 tcaggtgatc tgctgcatcc tcgcgacggg atggtcaatg ggcgtcgcgg ctctgctctg
     4441 ggttgtgctg cgtcggcggg gcgatcgcac gccgaggtcg gcatcgtgac cgctcccgag
     4501 ccgattgccg tgcagccgcc cgcccttcag ggtcgggtgg tcgcgagcca acggtggaca
     4561 cgactgacct ttctgcactg gcgggtcgag tcatcccgag tggcaccgct gctacccgcc
     4621 ggcgtgcgac cggacgagca cgacgggtcg agctgggtgg gcctgatcgc ctttcatctc
     4681 ggggatgcga cgatcgccgg cagcccgccc gtgccctact tcggcgactt cgccgaggtc
     4741 aacgtgaggt tgtacggtgt cgacgcgagc ggccgacgcg gagtcgtgtt cgcctccctc
     4801 gaggcgtcac gcctggccgc ggtcctggct gctcgtgcgg ctttctcgat cccctacttc
     4861 tggtcggcga cctcgctcat tgagacgggc ggcgcgatcg aatatggcgc gcgccgtcac
     4921 gtgggcgacg gagccactcg actggtcgcg cgaccgggtg ctgagctcgt catcggggat
     4981 gcgacggccg acttcctcac cgcccggtgg gggctcttca ccaggcggct cgggcgcacc
     5041 gtgttcctac cgaacactca cgaggcatgg cgcctgcaac gcgcgacact gctcgagctc
     5101 gatgacaccc tggtcgaacg ggcggggctt ccccatgtca ccgatcggat gcccgactcg
     5161 gtgctctacg ccgaaggggt cacggcgcgg ttcggtcgag ccctgaggta gcccgcgggt
     5221 cagtgcaagt ttgctggtca gtgcgagttc gctgatcagt ggactcgccg agcacacgcc
     5281 tcgcgagaag ggtcgcgccc ccgactgcgg ccggcatcat gatcaccgct ccgccgggaa
     5341 tcaggaacac cagccacgtc gcgatgccga agccgagcgt catggctctg cgaccgcgca
     5401 gcgcacgccg gcgttccgca agcgaacggc ctcgggaatc gaatgcgagt ccggtcagct
     5461 ccaccgtgag gtaccagccg ccgaagaatg cgcccagcac gggaacgacg acctgcccca
     5521 ccacgggaat gaacccgagc acgaagagca tcacgccgat gagtacggcg ggcacgagca
     5581 tccgcagacc cgaggagatg ccgcgaccca gctcgcgcca gaagcccgat gcgccctcgt
     5641 cgggtacggt gccgaacctg ccctccgtgt gccgccagat gcgctggtag aacgggtccc
     5701 cgacgatgag ggtgaccgcc gtgaacgtgt acacgaccag aaggatggcg accaccagga
     5761 acgcgaggcc tgcaaccaca tgcacggccg tgtaccacgg ctcgggccat tcgctcgcga
     5821 acggtgtgac cgcgacagcg atggcctcga tattgacgcc cagcgcgatc aggcccgcga
     5881 ggatgaccgc gcccacgatc agcgcgggca ccattcccag cagcatgaga ccgggcgcgg
     5941 tgcgccaagt gccgagtccg cggccgagta gggagacgcc ggtggcgaat tcgcgcaaca
     6001 cgctcggtct gccgtctcgc atcatgatcc cctcatgacg agtggtgggc gataccggac
     6061 tcgaaccgat gacctcttcc gtgtgaagga agcgcgctac caactgcgcc aatcgccc
//
LOCUS       DAOSOU010000119         6068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13136_length_6068_cov_5.327125, whole genome shotgun sequence.
ACCESSION   DAOSOU010000119 DAOSOU010000000
VERSION     DAOSOU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13136_length_6068_cov_5.327125"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLW15_07410"
     CDS             <1..460
                     /locus_tag="PLW15_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT34254.1"
                     /translation="LTLAIDNWRWAGVPFVLRSGKALGEARQEIVIVFRDVPHLPTGL
                     RGIAGPSALRISLKPATLDLDLVINGEGDPFTLDRTVLHTDLGAGELSAYGEVLSSLI
                     DADPTLSVRGDVAEECWRIVSPVLAAWRKDAVPLETYAAGSDGPNSWGRR"
     gene            complement(483..1271)
                     /locus_tag="PLW15_07415"
     CDS             complement(483..1271)
                     /locus_tag="PLW15_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34255.1"
                     /translation="MTEFVTSKDGTRIAYEKVGSGPALVLVDGAMCYRDFGPCRSAAT
                     AFSDRYTVYFYDRRGRGESGDSPEYAPEREYEDLAAVIDATGEEAYVLGYSSGGALVL
                     QAAASGVRMRKLASYEAPYVGIAEKKGVKPDFLAELNARLDRGDRGGAVGYFMVDMVG
                     GPAFLPLMMRLMPKVFTSLKAVAHTLPYDATIMHGFEVPTATLGRITTPALVMGGSKA
                     KPNMVAAVDGVASAIPGSIKRTLAGQTHQVKDDAIKPELVGFFA"
     gene            complement(1268..1735)
                     /locus_tag="PLW15_07420"
     CDS             complement(1268..1735)
                     /locus_tag="PLW15_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOT34256.1"
                     /translation="MTDRELPAVTDRDVYITRSFAAPIDVVWKFWTDPDLLVQWFGPH
                     MVTVPRESVEVDAREGGVWKLTMEDEYGVHPLTATILIAEPPTYLEMSLAADTGFGEL
                     DNVIVRVQLHDHGDTTRLTMHQGPFPDGFVEPTTQGWNESFDKLTTIFEGAQA"
     gene            complement(1728..2084)
                     /locus_tag="PLW15_07425"
     CDS             complement(1728..2084)
                     /locus_tag="PLW15_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT34257.1"
                     /translation="MPVDELSAVFAALADPTRRALLTRLEAGDASVAELGSPFAMSQP
                     AISQHLKVLENAGLISRTRVGTARLSHLEPAALKEASEYMERYKRFWNSAFDTLDHAL
                     AAYQEQAQAEKETPDD"
     gene            2128..2673
                     /locus_tag="PLW15_07430"
     CDS             2128..2673
                     /locus_tag="PLW15_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT34258.1"
                     /translation="MPAAAVKRRASHLATRVLRIAVMTRSSSAQATAVAQTTPKRAWE
                     ISGPLDPTKYYPKFGPLPAVVGVRDQTGPWDTVGQSRTLVLSDGSSVVETITDADSPT
                     FFAYELSNFTKLFGKLVAGARAEWRFERIDTGTSIRWTYTFHAKRGRGWIVAAIVRLF
                     WARYMTRVLPPIARAVESAAG"
     gene            complement(2678..3169)
                     /locus_tag="PLW15_07435"
     CDS             complement(2678..3169)
                     /locus_tag="PLW15_07435"
                     /inference="COORDINATES: protein motif:HMM:NF027885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FBP domain-containing protein"
                     /protein_id="HOT34259.1"
                     /translation="MEPLTPAEIRASFVNASQGEAERVPMPGLHDVIWEEREYLGWRD
                     QQSHQRGYLVHWLDDRPVGIVLRASEFSLQPGIAAMCALCRITRASDQVTMFSAARAG
                     QAGRDGNTIGTYICDDLACSHLIRILPPSSALQSQAQLLEQRIDGLLARVQGFTADVM
                     KTA"
     gene            complement(3278..3925)
                     /locus_tag="PLW15_07440"
     CDS             complement(3278..3925)
                     /locus_tag="PLW15_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT34260.1"
                     /translation="MTVDTAAPPRESYHHGDLRHALVLAGVELAREGGPEAVVLREAT
                     RRVGVSPNAAYRHFADREALLAAVCDAAQAELAAAIDESYWAVREPDAAATARAHLRA
                     VGTRYVAFALDNPGLFRTAFIASSDLERATSPERAGPSGLTPFQLVTAALDEFVASGI
                     MPASRRPNAEFLAWSSVHGLAMLLIDGPLRALPRGAAEAIAQQVIDMAEAGFTAP"
     gene            4002..>4756
                     /locus_tag="PLW15_07445"
     CDS             4002..>4756
                     /locus_tag="PLW15_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34261.1"
                     /translation="MTPTQPTQPTRRTLPRALTPFANGQYRILAVALTMSLFGSGVWL
                     VALVWQIIALGGSPIELSLVATGSAVGMILAVLFGGVLADRIPQKRILLAVEGAKVLV
                     VAGVAALALMGTLEVWHLVIAATILGVGEGFFYPAYSALLPSVLAADDLLAANGVEGV
                     LRPTVMQALGPAIASAAIAVASPALAFVIVAAAQVFAVAGLFFLHTTPVRRELGEPTH
                     PLKAFFGDIAGGFTYMFKTPWLLATLLYACLFVL"
     assembly_gap    4757..4856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4926..5309
                     /locus_tag="PLW15_07450"
     CDS             4926..5309
                     /locus_tag="PLW15_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT34262.1"
                     /translation="MFETSPAFSGFSVDDIDAALAFYGTTLGLKASLNHMGMIDLHLA
                     TGGHVIVYPKTNHEPATFTVLNFPVDEIDDAVDALVAAGVTFQRYEGIGQDEKGIARG
                     KAANQGPDIAWFTDPAGNILSVLSD"
     gene            5345..>6068
                     /locus_tag="PLW15_07455"
     CDS             5345..>6068
                     /locus_tag="PLW15_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT34263.1"
                     /translation="MDPALIARLAADLTAADFTVDGLTELWGEDAAAALHRGQRVPAS
                     RALTDSPLSTLARALVLGYPVDPAALDRALPTLTAAGAVTLGLVDAAGSPLLDLRPYS
                     VVDARGVAAWWIASDLGELALGHAIPEHHVLGVGGASTTLSGLMLAEPVELALDLGTG
                     CGIQALHAARHAKRVIATDISERALELARFNAALNGFANIEFRLGSLFEPVAGERFGH
                     VVSNPPFVITPRIDGVPEYEYRD"
BASE COUNT          879 a         2058 c         2046 g          985 t
ORIGIN      
        1 gctgacgttg gccatcgaca actggcgctg ggcgggtgtg ccgttcgtgc tgcggtcggg
       61 caaggcactg ggggaggcgc gacaggagat cgtcatcgtg ttccgcgatg tgcctcacct
      121 gcccaccggt ctgcgcggca tcgccggtcc gtcggcgctg cgcatctcgc tcaagccggc
      181 caccctcgac ctcgacctcg tgatcaacgg cgagggcgac ccgttcaccc tcgaccgcac
      241 ggtgctgcac accgacctcg gcgcggggga actcagcgcc tacggcgaag ttctgtcgag
      301 cctgatcgat gccgacccga cgctgtcggt gcgcggcgac gtcgctgagg agtgctggcg
      361 catcgtcagt cccgtgctcg cggcgtggcg caaagacgcg gtgccgctgg agacctacgc
      421 ggccggttcc gacggtccca actcgtgggg ccgccgttag cctgcgcggc tagggggagg
      481 cgctaggcga agaatcccac gagctcgggt ttgatcgcgt catccttcac ctggtgggtc
      541 tggcccgcga gagtgcgctt gatcgaaccg gggatggcgc tggccacccc gtcgaccgcg
      601 gcgaccatgt tcggcttggc cttgctgccg cccatcacga gcgccggggt ggtgatgcgg
      661 cccagcgtcg cggtgggcac ctcgaagccg tgcatgatcg tggcgtcgta gggcagggtg
      721 tgggcgaccg ccttgagcga ggtgaacacc ttcggcatca gccgcatcat gagcggcagg
      781 aacgccgggc cacccaccat gtcgaccatg aagtagccga ccgccccgcc ccggtcgcct
      841 cggtcgagcc gggcgttgag ctccgcgagg aagtcgggct tcacgccctt cttctcggcg
      901 attcccacgt agggcgcctc atagctcgcc agcttgcgca tgcgaacacc ggatgccgcc
      961 gcctgaagca cgagcgcgcc gcccgacgag taccccagca cgtaggcctc ctcaccggtc
     1021 gcgtcgatga ccgccgcgag gtcttcgtac tcgcgctcgg gcgcgtactc gggactatcg
     1081 ccgctctcgc cccggccgcg ccgatcgtag aagtacacgg tgtaccggtc ggagaaggcg
     1141 gtcgccgcgc tgcggcacgg gccgaagtcg cggtagcaca tcgcgccatc gacgagcacg
     1201 agcgcggggc ccgagcccac cttctcgtac gcgatgcggg tgccgtcctt cgacgtgacg
     1261 aattcggtca tgcctgtgct ccttcgaaga tggtggtgag cttgtcgaac gattcgttcc
     1321 agccctgggt ggtgggttcg acgaagccgt cgggaaacgg tccctggtgc atcgtcaggc
     1381 gggtggtgtc gccgtggtcg tgcagctgca cccgcacgat cacgttgtcg agctcgccga
     1441 atccggtgtc ggcggcgagc gacatctcga ggtaggtggg cggctcggcg atcaggatgg
     1501 tcgccgtgag cgggtgcaca ccgtactcat cctccatcgt gagcttccag acgccgccct
     1561 cgcgcgcatc cacctcgacg ctctcgcggg gcacggtcac catgtgcggt ccgaaccact
     1621 gcacgagcag gtcggggtcg gtccagaact tccagaccac gtcgatcggc gcggcgaagc
     1681 tgcgggtgat gtacacgtcg cggtcggtga cggccggcag ttctcggtca gtcatcggga
     1741 gtctccttct ctgcctgtgc ctgttcctgg taggcggcga gggcgtggtc gagggtgtcg
     1801 aatgccgagt tccagaagcg cttgtaccgt tccatgtact cactcgcctc cttgagggcc
     1861 gcgggctcca gatgactgag gcgcgcagtt cccacccgcg tgcgcgagat caggccggcg
     1921 ttctcgagca ccttgaggtg ctgcgagatg gctggctgac tcatggcgaa ggggctcccc
     1981 agttcggcga cgctcgcgtc ccccgcctcg aggcgcgtga gcagcgccct gcgggtgggg
     2041 tcggcgagtg cggcgaacac cgcgctcagc tcgtctacgg gcatccataa ccaatctatt
     2101 tcataagcaa ttgcctatag aatagggatg cccgcggccg ctgtcaagcg tcgcgcctcc
     2161 catctcgcaa cccgcgtgct acgaatagca gttatgacac gaagctcgag cgcccaggcg
     2221 accgcggtag cccagaccac accgaagcgc gcctgggaga tctccggccc gctcgacccc
     2281 acgaagtact acccgaagtt cggacccctt cccgcggtcg tcggcgtgcg ggatcagacc
     2341 ggcccgtggg acaccgtcgg tcagagtcgc acgctcgtgc tttccgatgg cagctcggtg
     2401 gtcgagacga tcaccgacgc cgactcgccc accttcttcg cctacgagct gagcaacttc
     2461 accaagctgt tcgggaagct ggtcgccggg gctcgcgccg agtggcggtt cgaacgcatc
     2521 gacaccggca cgagcatccg gtggacctac acgttccacg ccaagcgcgg ccgcggctgg
     2581 atcgtcgcgg caatcgtgcg cctgttctgg gcgcgctaca tgacccgcgt gctgccgccg
     2641 atcgcgcggg cagtcgagtc agcggcgggc tgagttccta tgccgtcttc atcacgtcgg
     2701 cggtgaagcc ctgcacgcgc gcgagcagcc cgtcgatgcg ctgctcgagc agctgcgcct
     2761 gactctgcag ggctgacgac ggcggcagga tgcggatgag gtgcgagcac gcgaggtcat
     2821 cgcagatgta ggtgccgatc gtgttgccat cgcgacccgc ctggcctgcg cgggcagcgg
     2881 agaacatcgt cacctggtcg ctcgcccggg tgatgcggca cagcgcgcac atcgccgcga
     2941 tgcccggctg aagcgagaat tcggaggccc gcagcacgat gccgacaggc cgatcgtcga
     3001 gccagtgcac caggtagccg cgctggtggg actgctggtc gcgccagccg aggtactcgc
     3061 gttcctccca gatcacgtcg tgcaatccgg gcatgggaac gcgctcagcc tcaccctgtg
     3121 acgcattcac gaagctcgcg cggatctccg ccggggtgag gggctccatc cctccagcgt
     3181 acatacccgc gccgttgcgg aggatgacac gcgacgtgac cgacagtgtt gtgcaccgcg
     3241 tcggcgagtc atcctccgcg actgtggtgc ggacgggtta cggcgcggtg aagcccgcct
     3301 ccgccatgtc gatgacctgc tgcgcaatcg cctccgccgc cccccgcggc agcgcgcgca
     3361 gcggtccgtc gatcagcagc atggcgagcc cgtgcacgct cgaccaggcg aggaactccg
     3421 cgttcggtcg ccgcgacgcc ggcatgatcc cggatgccac gaactcatcg agcgccgccg
     3481 tgacgagctg gaacggggtg aggccgctgg gcccggcccg ctcggggctg gtcgcgcgct
     3541 ccaggtcgga cgacgcgatg aacgccgtgc ggaacagtcc cggattgtcg agcgcgaagg
     3601 cgacgtagcg ggtgccgacg gcgcgcaggt gcgcgcgggc ggtcgccgcg gcatccggct
     3661 cgcgcaccgc ccaatagctc tcgtcgatcg cggccgccaa ctcggcttga gctgcgtcgc
     3721 acacggcggc gagaagtgct tcgcggtcgg cgaagtgacg gtaggcggcg ttgggtgaga
     3781 cgccgaccct gcgggtggct tcgcggagga ccaccgcctc ggggccgccc tcccgcgcga
     3841 gctccacgcc ggccagcacg agagcgtgtc ggaggtcgcc gtgatgatag ctctcacgcg
     3901 gcggtgcggc cgtgtccacg gtcatagcgc ctgtccttcc ggtggacgat gccctcatcc
     3961 tatgacagtg tttacaccgt aaacatgatt gtgagcaggg gatgacgcca acgcagccaa
     4021 cgcagccgac gcgccgcact ctgccgcggg cgctcacgcc gttcgccaac ggccagtacc
     4081 gcatcctcgc tgtcgcgctc accatgtcgc tgttcggctc gggcgtctgg ctggtggcgc
     4141 tggtctggca gatcatcgcc ttgggcggct cgccgatcga actttcgttg gtagccaccg
     4201 gcagcgctgt cggcatgatc ctcgcggtgc tgttcggggg agtgctggcc gatcgcatcc
     4261 cgcagaagcg catcctgctc gccgtcgagg gcgcaaaagt gctggtcgtg gccggtgttg
     4321 ccgccctcgc gctgatgggc accctcgaag tctggcacct cgtcatcgcg gccacgatcc
     4381 tgggggtcgg cgaggggttc ttctaccccg cctactcggc gctgctgccg tccgtgcttg
     4441 cggccgacga cctgctggcc gcgaacgggg tcgaaggggt gctgcggccg acggtcatgc
     4501 aggcgctggg accggcgatt gcgagcgcgg cgatcgccgt ggcatccccg gccctggcat
     4561 tcgtcatcgt ggcggccgcc caggtgttcg cggtggcggg gctgttcttc ctgcacacga
     4621 cacccgtgcg gcgcgagctg ggggagccga cgcatccgct caaggccttc ttcggcgaca
     4681 tcgccggcgg cttcacctac atgttcaaga cgccgtggct gctggccacc ctgctctacg
     4741 cgtgcctgtt cgtgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg
     4861 acccctcgag ccaggagatc ctcggcccgg gaacgcccat tcagggctga cagaaggagc
     4921 acaccatgtt cgaaacgtca ccggccttca gcggattctc cgtcgacgac atcgacgccg
     4981 cgctcgcgtt ctacggcacc accctggggc tgaaggccag cctcaaccac atggggatga
     5041 tcgatctcca cctcgcgacc ggcggtcacg tcatcgtcta cccgaagacc aaccacgagc
     5101 cggcgacgtt cacggtgctc aacttcccgg tcgacgagat cgatgacgcg gtcgacgctc
     5161 tggtggccgc cggcgtgacc ttccagcggt acgagggaat cggccaggac gagaagggca
     5221 tcgcgcgcgg caaggcggcg aaccagggac cggacatcgc ctggttcacc gacccggcag
     5281 gcaacatcct gagtgtgctc tcggactgag tgatcggccg ctcgcggact gattgactgg
     5341 gggcgtggat cccgcgctca tcgcccgtct cgccgccgac ctgaccgcgg cggacttcac
     5401 cgtcgacggc ctcaccgagc tgtggggcga ggatgccgcg gcagccctac accgtggcca
     5461 gcgggtaccg gcgtcccgcg cgctcaccga ctcgccgctc tcgactctcg cccgcgcgct
     5521 ggtgctcgga taccccgtcg acccggcagc cctcgaccgc gcgctgccca ccctcactgc
     5581 cgccggcgcg gtcacgctcg gactcgtgga tgctgcgggc tcccccctgc tcgacctgcg
     5641 gccgtactcc gtggtcgacg cccgcggcgt cgccgcgtgg tggatcgcca gtgacctggg
     5701 cgagttggcg ctcggccacg cgatacccga gcatcacgtg ttgggggtgg gtggggcatc
     5761 cacgactctg agcgggctga tgctcgccga gccggtcgag ctcgcgctcg acctgggcac
     5821 cggctgcggc atccaggcgc tgcatgcggc ccggcacgcg aagcgcgtga tcgccacgga
     5881 catctccgag cgcgcgctgg agctcgcccg gttcaacgct gcgctcaacg ggttcgccaa
     5941 catcgagttc cggctcggca gtctgttcga gccggtcgcg ggggagcgct tcggacacgt
     6001 ggtgagcaac ccgccgttcg tgatcacgcc gcgcattgac ggggtgcccg agtacgagta
     6061 ccgcgacg
//
LOCUS       DAOSOU010000120         6054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13182_length_6054_cov_5.197533, whole genome shotgun sequence.
ACCESSION   DAOSOU010000120 DAOSOU010000000
VERSION     DAOSOU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13182_length_6054_cov_5.197533"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLW15_07460"
     CDS             <1..999
                     /locus_tag="PLW15_07460"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT34264.1"
                     /translation="RGIIVAQYSEPENLSILQAAHLVGRGSAALPAALVRLAVRHNLR
                     ILAYPVDASISEPYTLQYLSSAGADTTDLELLGDLFGADPQPGALKGFGHRDQLLMAR
                     LEAASSKANASLLAEGYQRKPRGVAAGILIPLVEFLLIFVGMGLLMLMAFVFLNVSPL
                     VVIAMVFAFFAVFITLPLAWRSRTVTDKGADARDYVKGMKVYLDLAEKDRLQMLQSAT
                     GADRVDVGDNLQLVKLYEKLLPWAVLWGVETSWMKELAVHVDALDEKPDWFVGTNGFE
                     VSAFTSTLGGFRTTLTPPPSTSSSGWRGSSGGSFSGGSHGGGFSGGGGGGGGGGGR"
     gene            complement(1033..1530)
                     /locus_tag="PLW15_07465"
     CDS             complement(1033..1530)
                     /locus_tag="PLW15_07465"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOT34265.1"
                     /translation="MTTTLADIPLTTITGEATSLGAFAGKVLLIVNVASRCGLAPQYE
                     KLEALQKKYESRGFTVLGFPSNQFLQELGKNEAIAEYCSATWGVTFPMFDKVKVNGKG
                     AHPLYQELVKAPYDGSSKDKGGKIKWNFEKFVITPSGDVHRFLPNMEPDDPAIVSVIE
                     SALPA"
     gene            1584..2495
                     /locus_tag="PLW15_07470"
     CDS             1584..2495
                     /locus_tag="PLW15_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT34266.1"
                     /translation="MSESPLSLSPYEVLGVAASVSDDELRKAFRKALRETHPDTGGDP
                     KRFTAVQLAWERIGSPEKRAAYDAGRSTRGDHPTFTAQPARPRQDTRPKPRSYGHPGG
                     WRRERFLSQMREWVGRGVTLDDPYDPALVRTAPREIRHTLADALAEEATARTLSTLGI
                     GYTVWHDVATGAPEDKIDHIVLGPTGLVAMLSEDFGGPVRVRKNELIGEAVAGERPVH
                     ELAIRAKVISRQLRVRFSALIIVLPDDALDEPIVSLGSVRGAAAAAVRQSVLAGVLRN
                     GLPGAQPIGGNELFDVRTRLVGGIRFV"
     gene            complement(2498..2653)
                     /locus_tag="PLW15_07475"
     CDS             complement(2498..2653)
                     /locus_tag="PLW15_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34267.1"
                     /translation="MSDSTGIAGPGDAFDDETQPHHPKHRRDDVPVDAEPLLSEFGEN
                     PDTSATG"
     gene            2730..3992
                     /locus_tag="PLW15_07480"
     CDS             2730..3992
                     /locus_tag="PLW15_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOT34268.1"
                     /translation="MSIRPALARRLGPTDATLIGLGSMIGAGVFAAFTPAAQAAGSGL
                     LVGLAVAALVAFANATSSAQLAAVYPTSGGSYVYGRERLGHWWGFFAGWSFVIGKTAS
                     CAAMALTFAAYAAPAGWERPVAIVAVVALATVNLFGVTRTALLTRVIVVVVLVVLAVV
                     VAAGASTGLPALAVTGSLVAGGWYGILQSAGLLFFAFAGYARIATMGEEVRDPARTIP
                     LAITLALSATVLIYAVIGVVVLGVLGPDGVAGTPTPLSATVGAAGWAWATPAVRVGAA
                     AASLGALLALVAGIGRTTLAMARERDLPGWLAAVHPRFSVPYRAEIALALVVIVLVGT
                     VDLRGAIGFSSFGVLLYYVIANASAITQPREQRRYPRALQIFGIAGCLALVITLPPLS
                     IVVGVAVLLAGFAYRLLSRRAMAPNHPQ"
     gene            4034..4732
                     /locus_tag="PLW15_07485"
     CDS             4034..4732
                     /locus_tag="PLW15_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT34269.1"
                     /translation="MRVISYNLRKHRAVGELAELAKTPDLDALCLQEVFAAELPAEIG
                     NLHLADATKSNRLGLAVYYRKDRYEQVASQSFELKKSLHDLIFSPTPERLVGSRLVDR
                     GTGHELVLASFHAAPLTALNSLRRTQIKAAHELLQQLGDGAPIIMVGDYNYPLFKDNL
                     SERVKKTGYDLSLSDRRTYTAYKFFRGHFDFVTSQGFTIEGVETLARGSSDHIPILVT
                     ASYGEPAYKRAGQE"
     gene            4793..5251
                     /locus_tag="PLW15_07490"
     CDS             4793..5251
                     /locus_tag="PLW15_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34270.1"
                     /translation="MTIASRLRAALGWIVIAVLAGVAVALGTILIAGVSVADEAGSSI
                     GVPAVKVTGEPHPVSATAESSAAPGLEPSEVGAAGSDTADGTSPDTQVVAAPEPISVT
                     LDDHGGASGSDGGGSESGSSGGSGSSNGSGSSGGSGGSGSNSGSSGGGSK"
     gene            5367..5825
                     /locus_tag="PLW15_07495"
     CDS             5367..5825
                     /locus_tag="PLW15_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34271.1"
                     /translation="MSAFSTRGAITAGAVAVAIVLSIGGVAVAQASIDSSPFDSSPSP
                     SPTLTDEPATHDLGDDNGVDDPATHDVGDDNGVDDPATHDVGDDDGVDSDDIGDDNGS
                     DSDDVSDDNDSDDSDNSGTGSDSSGHGSDGSESTDNSGHGSENSGSGSDH"
     gene            complement(5859..>6054)
                     /locus_tag="PLW15_07500"
     CDS             complement(5859..>6054)
                     /locus_tag="PLW15_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT34272.1"
                     /translation="EFALAEELMRNADRVLSREQLLSRVWGYDFDPGSNVVDVYVGYL
                     RAKLGAAHIETVRGMGYRLR"
BASE COUNT          965 a         2030 c         1992 g         1067 t
ORIGIN      
        1 cggggcatca tcgtggcgca gtacagcgag cccgagaacc tgtcgatcct gcaggccgcc
       61 cacctcgtcg gccggggctc ggcggcgttg ccggcggcgc tcgttcgact ggccgtgcgc
      121 cacaacctgc gcatcctggc ctacccggtg gacgcgtcga tcagtgagcc ctacacactg
      181 cagtacctgt cgagcgccgg agccgacacc accgacctcg aactgctggg cgacctgttc
      241 ggagccgatc cgcagccggg cgcgctcaaa ggcttcggtc accgcgacca actcctgatg
      301 gcgcggctcg aggctgcgtc atccaaggcc aacgcatcat tgctggccga gggctaccaa
      361 cgcaagccga ggggcgtcgc ggcgggcatc ctgattcccc tggtcgagtt cctcttgatc
      421 ttcgtgggga tgggcctgct catgctgatg gccttcgtct tcctcaacgt cagtccgctg
      481 gttgtcatcg ccatggtctt cgcgttcttc gccgtcttca tcacgctgcc gctggcgtgg
      541 cgctcccgca cggtcaccga caagggtgcg gatgcccgtg actacgtcaa gggcatgaag
      601 gtgtacctcg atctcgccga gaaggaccgc ctgcagatgc tgcagagcgc gacgggagcc
      661 gacagggtgg atgtgggcga caacctgcaa ctcgtgaagc tctacgagaa gctgctgccc
      721 tgggcagtgc tgtggggtgt ggagacgagc tggatgaagg aactcgcagt gcacgtggat
      781 gccctggacg agaagccgga ctggttcgtc ggcacgaacg gcttcgaggt ctcagccttc
      841 acgagcacac tcggcggatt ccgcaccact ctgacaccgc cgccatccac gagttcatcg
      901 ggttggcgcg gcagttcggg cggaagcttc tccggtggtt cgcacggggg agggttctcc
      961 ggtggaggtg gcggcggggg aggaggcggc ggccgttagg tccgctggcc ggtggtgcac
     1021 gggctccgcg gctcaggcgg gcagtgccga ctcgatcacc gagacgatgg ccgggtcatc
     1081 cggctccatg ttcggcagga accggtgcac gtcacccgac ggggtgatca cgaacttctc
     1141 gaagttccac ttgatcttgc cgcccttgtc cttggagctg ccgtcgtagg gtgccttcac
     1201 gagctcctgg tagagcgggt gtgcgccctt gccgttgacc ttgaccttgt cgaacatcgg
     1261 gaaggtgaca ccccaggtcg ctgagcagta ctcggcgatc gcctcgttct tgccgagctc
     1321 ctggaggaac tggttactgg ggaagccgag caccgtgaac ccgcgcgact cgtacttctt
     1381 ctgcagcgcc tcgagcttct cgtactgcgg agcaaggccg caccgggaag cgacattgac
     1441 gatgaggagc accttgcccg caaaggcacc gaggctcgtg gcctcgcccg tgatggtggt
     1501 cagtgggatg tcggcgagag tagtagtcac gtcaccaatc tacgcccagc ttgctggatg
     1561 ccagccgccg gtatcctgag tcggtgtcgg agagcccctt gtcgctgagt ccctacgagg
     1621 tgctcggggt tgcggcatcc gtgagcgatg acgaacttcg caaggcattc cgcaaggcgt
     1681 tgcgggaaac gcaccccgac accggcggcg acccgaagcg gttcaccgct gtgcaactcg
     1741 cctgggagcg catcggcagc ccggagaagc gcgcggccta tgacgcgggg cgcagcactc
     1801 gtggcgacca cccgacgttc accgcacagc ccgcacgacc gaggcaggac acccggccca
     1861 agccgcgcag ctacgggcat cccggcggat ggcgccgcga gcgctttctc tctcagatgc
     1921 gcgagtgggt gggtcgcggt gtcacactcg atgatccgta cgacccggcg ctggtgcgca
     1981 ccgcgccgcg cgagatccgg cacactctgg ccgacgcgct tgccgaggag gcgaccgcac
     2041 gcaccctgag cacgctgggc atcgggtaca ccgtgtggca cgatgtggcg accggcgcac
     2101 ccgaggacaa gatcgatcac atcgtgctcg gccccaccgg cctcgtcgca atgctctccg
     2161 aggacttcgg cgggcctgtg cgggtgcgca agaacgaact catcggggag gcagtcgccg
     2221 gggagcggcc ggtacacgaa ctggctatcc gcgcgaaggt gatctcccgc caattgcggg
     2281 tcagattcag tgctctcatc atcgtgctac cggatgacgc cctcgacgaa ccgatcgtct
     2341 ctctgggcag cgtccgcggg gccgcggcgg cggctgtgcg tcagtcggta ctggcgggcg
     2401 ttctgcgcaa cggacttccg ggtgcccaac cgatcggggg caacgagttg ttcgacgtgc
     2461 gaacccgact cgtgggcggc atccgcttcg tctgagttca gccggtcgcc gaggtgtccg
     2521 ggttctcgcc gaattcgctc agcagcggct cggcgtccac gggcacatcg tcgcgccggt
     2581 gcttcgggtg gtgcggctgc gtctcgtcgt cgaacgcgtc gccgggcccc gcaatcccag
     2641 tgctgtcact cattctgacc tccccagtca agtgaattgc tcagattact ccaccgatgc
     2701 cgttcacccg caagcggtag ggtgcgcctg tgtccatcag gcccgctctc gccagacgcc
     2761 tcgggccgac ggatgccacg ctcatcggtc tcggctccat gatcggtgcc ggggtcttcg
     2821 ccgccttcac tcccgccgcg caggcggccg gatcgggact gctcgttggc ctcgcggtgg
     2881 cagccctcgt cgccttcgcg aatgcaacct cgtctgccca gctcgctgcc gtgtatccca
     2941 cgtccggcgg cagctacgtc tacggccgtg agcgcttggg gcactggtgg ggcttcttcg
     3001 ccgggtggag cttcgtcatc ggaaagaccg catcgtgtgc ggcgatggcg ctcaccttcg
     3061 ccgcctacgc tgcgcctgcg ggctgggagc gaccggtggc gatcgtcgcg gtcgtcgcgc
     3121 tggcgaccgt gaacctcttc ggcgtcactc gcaccgcgct gctgacccgc gtgatcgtgg
     3181 tggtcgtgct cgtcgtgctc gcggtggtgg ttgcggctgg ggcatccacc gggctgccgg
     3241 cactggccgt gacgggcagc ctcgtcgccg gcggttggta cggaatcctg caatcggcag
     3301 gactgctgtt cttcgccttc gcgggctacg cccggatcgc gaccatgggg gaggaggtgc
     3361 gagaccccgc tcgcacgatc ccgctcgcga tcacgcttgc gctgagtgcg acagtgctca
     3421 tctacgccgt gatcggggtg gtggtactcg gagtgctggg gcccgacggt gtagccggaa
     3481 cgccgacacc gctgtcggca acagtgggcg ccgcggggtg ggcctgggcg acgcccgcgg
     3541 tgcgggtggg tgccgcggcc gcctcgctcg gcgcgctcct cgccctcgtc gctggcattg
     3601 gtcgcaccac gctcgcaatg gcacgggagc gcgatcttcc cgggtggctt gcggccgtgc
     3661 atccgcgatt ctctgtgcca taccgggcgg agatcgcgct cgcccttgtc gtgatcgtgc
     3721 tcgtgggcac tgtcgacctg cgcggcgcga tcggtttctc gtcgttcggg gtgctgctgt
     3781 actacgtgat cgccaatgcg tcggcgatca cccagccccg cgagcagcgt cgctatcctc
     3841 gtgcactcca gatcttcggc atcgccggat gcctcgcact cgtgatcacc cttccaccgc
     3901 tgtcgatcgt ggtcggcgtc gccgttctgc tcgcaggttt cgcgtatcgg ctcctatccc
     3961 gtcgtgccat ggcaccgaac cacccgcagt aggctggaca cttcgccaag gaagaggaac
     4021 gcgaaacccc gtgattcgag tcatcagtta caacctgcgc aagcaccgcg ccgtcggcga
     4081 acttgccgaa ctcgcgaaga cgccggatct cgacgctctc tgtctccagg aggtgttcgc
     4141 ggcggagctg cccgcggaga tcggtaacct gcacttggcc gacgcgacca agagcaaccg
     4201 gttgggcctc gcggtctact atcgcaagga ccgctacgag caggtggcga gccagtcgtt
     4261 cgagctgaag aagtcgctgc acgatctgat cttctccccg accccggagc gactcgtcgg
     4321 ctcgcgactc gtcgaccggg ggaccggcca cgagctggtt ctcgcctcct tccacgcggc
     4381 accgctcacc gcactcaatt cgctgcgccg cacccagatc aaggcggctc acgaactgct
     4441 gcagcaactc ggcgacggcg caccgatcat catggtcggc gactacaact acccgctctt
     4501 caaagacaat ctcagcgagc gggtcaagaa gaccggctac gacctctccc tgagcgaccg
     4561 gcgcacctat accgcgtaca agttcttccg cgggcacttc gatttcgtca cctcgcaggg
     4621 tttcacgatc gaaggggtgg agactctcgc gcgaggctcg agcgatcaca tcccgattct
     4681 cgtcaccgcg tcgtacggcg aaccggcata caaacgggct ggccaggagt gagctcctcc
     4741 gcctaagagg attctcatcc cttgtcgcgc aacgtcgcga cacaatgggg tcatgacaat
     4801 cgcgtcccgt ctgcgcgccg ccctcgggtg gattgtcatc gctgtgcttg cgggcgtcgc
     4861 ggtcgcactc ggcaccattc tcatcgctgg agtgagcgtg gcggatgagg cggggtcatc
     4921 cataggggtg ccggccgtca aggtcaccgg cgaaccgcat ccagtgtcgg caacggccga
     4981 atcttccgcc gcccccgggt tggagcccag tgaggtcggt gctgcgggct cggatacggc
     5041 agacgggacc agcccggaca cccaggtggt tgccgcaccc gagccgatct cggtgactct
     5101 ggatgatcac ggcggagcca gtggcagtga cggcggtggg tccgagtccg ggtcgtcggg
     5161 cggctcgggt agctcaaacg gatcggggtc gtcgggcggc tcgggcggat cgggatcgaa
     5221 ctccggttcc agcggcggcg gaagtaagta agaactgtct catccagccc tcgtagctgc
     5281 cttactttcg accgtcagtc tgtgatcacg gcacaacgcc gcacaccgcc gccgggggat
     5341 ccggcaccag tgaagggttt ccgcacatgt cagcattcag cacccgaggc gctatcaccg
     5401 caggcgcggt ggcggtcgcc atcgttctgt ccatcggcgg agtcgccgtc gcccaggcat
     5461 ccatcgattc cagcccgttc gattccagcc cgtcgccctc gccgactctc accgacgaac
     5521 ccgcaaccca cgacctgggt gacgataacg gcgtcgacga cccggccacg catgacgtcg
     5581 gtgacgacaa tggcgttgac gatcccgcga cccacgatgt tggcgacgac gacggtgtcg
     5641 actcggacga catcggtgac gacaacgggt cagactcaga cgacgtgagc gacgacaacg
     5701 actccgacga ctccgacaac agtggcaccg gcagcgacag cagtggccat ggctcggacg
     5761 gcagcgagag cactgacaac agcggtcacg gctccgagaa cagcggctcg ggaagcgatc
     5821 actgactcct cccgaagcac cccgaatggg ctatcgactc accgcagtcg gtagcccatt
     5881 ccgcgtaccg tctcaatgtg cgcagccccc aacttcgccc gcaggtaacc gacgtacacg
     5941 tcgacgacgt tggagcccgg gtcgaagtcg tacccccaga ccctgctgag caactgctca
     6001 cggctgagca cccgatcggc gttgcgcatg agctcctccg ccagcgcgaa ttct
//
LOCUS       DAOSOU010000121         6043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13221_length_6043_cov_5.932031, whole genome shotgun sequence.
ACCESSION   DAOSOU010000121 DAOSOU010000000
VERSION     DAOSOU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13221_length_6043_cov_5.932031"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLW15_07505"
     CDS             complement(<1..396)
                     /locus_tag="PLW15_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34273.1"
                     /translation="MTETLHFDDVSAVALETDDEIVARVRELVRNAIRHQVWLMFLNA
                     ERRQLPVIMPTDVPPAPDEDDPSRIGAFIRELAAELEASTVVVTYERVGGDQLTDADR
                     VWLRALHEACENSRVAFSGPHLCHRRGVRA"
     gene            complement(483..1031)
                     /locus_tag="PLW15_07510"
     CDS             complement(483..1031)
                     /locus_tag="PLW15_07510"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HOT34274.1"
                     /translation="MTFQAYLDAIKAKTGLDPADFKRLASEKGILDAKASVVLAWLKE
                     DFELGSGHGMAIFGTFKDRPEASERVDKQFAGPKAHWRPVVDQLIATLAEHGPVGTAP
                     TDTYISLLNGKAKFAIVAMTADRLDVGIKLRDAATMATSPRVEPSGSWNSMVTHRVRV
                     TDPAQVDAELIGWLRAAYDAAG"
     gene            complement(1085..1696)
                     /locus_tag="PLW15_07515"
     CDS             complement(1085..1696)
                     /locus_tag="PLW15_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DarT ssDNA thymidine ADP-ribosyltransferase
                     family protein"
                     /protein_id="HOT34275.1"
                     /translation="MNLLDQRVFHVTHLRNLEGIVGAGGLVAGESPEVDFSSPLAREL
                     RSSATVGEGGAQPVSAYVPWYLTPDAEVWNELRRGAAEPRWSAAARAAASVDLVVLVS
                     TVKALGDLADGDIVVADGDAAGTYTRFAGGADPALRMLARLHNTDHALAAEVLVKDSV
                     PFEAVQLIGVANEPQRDRVRALLDAAGVRTKVAVYPPWFAAPA"
     gene            1919..2542
                     /locus_tag="PLW15_07520"
     CDS             1919..2542
                     /locus_tag="PLW15_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOT34276.1"
                     /translation="MTDSETVPAPIADLTKGIWWWVLIRGILAIAFGIIALINPGSAI
                     LAFAIVFGAYALVDGIFSAVHAVRVRNQLKAWGWLLAQGILSAIAGLIALIIPGLVGI
                     FGGLVLLWTIVIWNITHGVAGIRSAAGAPSGTAKTVGIIAGVLSILFGILLGVLILVT
                     PGAALLGLVWLIGIYAIVFGIMLVVAAIQSRAGSGAKAGAKGTPAAA"
     gene            complement(2539..3678)
                     /locus_tag="PLW15_07525"
     CDS             complement(2539..3678)
                     /locus_tag="PLW15_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT34277.1"
                     /translation="MPTDRPTAHAAGSDVDRVLLMGNGIAVSYGVLSHDLGLAGHLAR
                     RLSALTGRGTTVDVRAAPDLTPADALPILASPEFAKYDAVVSTLGGLEAVLFGSADVW
                     RHSVRELIETLARSAPNTSIHFVGIPLSSNMVRMPRLYGLAVEAHTNRLNTITEQVCA
                     EYPSARFIPFEPVQARVLDQIGRDLYKRWADPIAESVAHKLDGRVHRDTIPTDEDARL
                     AALVEMGILDSPRDEELDQIVATARDLFGASGAGVNLIDSDRQWVMSSAGRSHGTICR
                     GDSVCTTTIGAADMFVVEDTLLNPDYRDKEWAHGDDAVRFYAGYPLEAPDGHRIGALC
                     VVDDKPRTFEATDRALLRDLAMRVQALLWEKRSTLAAPTQGEQHR"
     gene            complement(3830..4204)
                     /locus_tag="PLW15_07530"
     CDS             complement(3830..4204)
                     /locus_tag="PLW15_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5997 family protein"
                     /protein_id="HOT34278.1"
                     /translation="MKPETAAKKLGIYLQAAPAEFREGLVTRTQFLELQANPPEWLVE
                     LRRTGPHPKQVVAQKLGISVSGLTRAGAPTSMTTAEIKQLLEQKPEWLVVERATQAAV
                     HEENARIKFERRAKSEHRQGGV"
     gene            4260..4919
                     /locus_tag="PLW15_07535"
     CDS             4260..4919
                     /locus_tag="PLW15_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HOT34279.1"
                     /translation="MSEPFVVAFVVGVTPAKWARVWGERMPRHPLTLLPTGDALAALD
                     DGTAQVALTRLPVDAERFSVIPLYTEQAVVVAPKDHAIEALDALTLADLADETVLVGE
                     WAPTIELVAANVGVTVLPQSVARALSRRDVIARPVTDAPTTQVALVWPIDAPSDLIDE
                     FIGIVRGRTANSSRGQVAVPEQKPAAKPAAKPGAKSGAKPAARAGQKPRRGAPPRPRK
                     R"
     gene            complement(4931..5287)
                     /locus_tag="PLW15_07540"
     CDS             complement(4931..5287)
                     /locus_tag="PLW15_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HOT34280.1"
                     /translation="MTKNWTPLAITWFVLAIAGLVGTWTFNALAIVQLRDFVGDWVNS
                     GPAVSSLTVDLLVVAVAGCILIVVESRRLGMRGAWLYIVLSGLTAFAFTFPLFLAMRQ
                     RRLTAIAADAQQLPRT"
     gene            5348..5749
                     /locus_tag="PLW15_07545"
     CDS             5348..5749
                     /locus_tag="PLW15_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34281.1"
                     /translation="MTDATADPTPPAPPVTASGRPPRRSATPGVWALAVTLFPLLYTG
                     VGLILLAFGQGANGIGGFAEAAYVMFFLGFLVVPACLIVGLVLGIIALAFSRPLGKVL
                     GGIALVLIVAGVVALTLLLMGSNSPLFWTDF"
     gene            5837..>6043
                     /locus_tag="PLW15_07550"
     CDS             5837..>6043
                     /locus_tag="PLW15_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34282.1"
                     /translation="MSSALAVAPAHEAVTTAETAKTADTVQVADTVQALQERIRSMQA
                     TKLDSRLLPTHPAIAELLPGGGLQQ"
BASE COUNT          921 a         2051 c         2029 g         1042 t
ORIGIN      
        1 ggcccgaacc ccgcgccgat ggcacaggtg tggtccgctg aacgcgaccc tcgagttctc
       61 gcaggcctcg tgcagagcgc gcagccacac gcggtcggcg tcggtcagct ggtcgccgcc
      121 gacacgttca tacgtgacca cgacggtgct cgcctcgagt tctgcggcca gctcacgaat
      181 gaacgccccg atgcgcgacg ggtcgtcctc atccggcgct ggcggcacat ccgtcggcat
      241 gatcaccggc agttgccgcc gctcggcgtt gaggaacatc agccagacct ggtggcggat
      301 cgcattgcgc acaagctcgc gaacgcgtgc cacgatctcg tcgtcggtct cgagcgccac
      361 ggcgctcacg tcatcgaagt gaagtgtctc ggtcatgccc cgagtgtgcg gtcacggagg
      421 ccgcacccgg ggtcacgaga agggatctgt ggactggcgt ccctgtgcac tttctgcgtg
      481 tgtcagccgg ccgcgtcgta cgccgcgcgc aaccagccga tcagctcggc atcgacctgc
      541 gccggatccg tcacccgcac gcgatgggtg accatcgagt tccaggagcc gctcggctcg
      601 acgcggggag aggtggccat ggtcgcggca tcccgcagct tgatgccgac atcgagccgg
      661 tcggcggtca tcgcgacaat cgcgaacttg gccttgccgt tgagcaggct gatgtatgtg
      721 tcggtgggcg ccgtgccgac gggtccgtgc tcggcgagag ttgcgatcag ttggtcgacg
      781 accggtcgcc agtgggcctt cggcccggcg aactgcttgt cgacacgctc ggatgcctcg
      841 gggcggtcct tgaacgttcc gaagatcgcc attccgtggc ccgagccgag ctcgaagtcc
      901 tccttcagcc aggcgaggac gacgctggcc ttcgcatcca ggatgccctt ctcgctggcc
      961 agccgcttga agtcggcggg gtcgagtccc gtcttggctt tgatcgcgtc gaggtaggcc
     1021 tgaaaagtca tggggtcgag tgtaggccgt tgatgaatcg gccgagatga tgtctcgctg
     1081 ctggtcaggc cggcgctgcg aaccacggcg ggtacaccgc caccttggta cgcacgcctg
     1141 ccgcgtcaag gagggcgcgc acacgatccc gctgcggctc attggcgacg ccgatcaact
     1201 gcaccgcctc gaacggcacc gagtccttca cgagcacctc ggcagccaga gcgtgatcgg
     1261 tgttgtgcag gcgggcgagc atccgcaggg cggggtcggc gccaccggcg aaccgggtgt
     1321 acgtgccagc cgcatcacca tcggcgacca cgatgtcgcc gtcggccaga tcgccgagcg
     1381 ccttcacagt gctcacgagc accaccaggt cgacggatgc cgcggcacgc gccgcagccg
     1441 accaccgggg ttcggcggct ccccggcgca gctcgttcca gacctcggcg tcgggcgtca
     1501 ggtaccacgg cacatacgcg gacacgggtt gcgcgccgcc ctcccccacc gtcgcggatg
     1561 accgcagctc gcgggccagc ggcgagctga agtcgacctc gggcgactcg ccggcgacca
     1621 gcccgccggc gccgacgatg ccctcgaggt tgcgcaggtg agtcacgtgg aagacgcgct
     1681 gatcgagcag gttcacggat ggtgcgctcg ccgctgatcg gggtgcggcc gatcgtatgg
     1741 gagcgacacg cggcgcggca ctgcgagcag cccggacagg gctgacccgg gatgcccggg
     1801 gcggcaccac cggcgtcttg gggtagcaga tgtcgcacat acccgcttcg aatccgtgga
     1861 tgcattctgc cgaagtcaca tgtccacgtt acttcgcctc gctcgctata ttgttcgcat
     1921 gaccgactca gagaccgttc ccgcccccat cgccgacctc accaagggca tctggtggtg
     1981 ggtgctcatc cgcggcatcc tggctatcgc tttcggaatc atcgcgctca tcaacccggg
     2041 atcggcgatc ctggccttcg ccatcgtctt cggcgcctac gcgctcgtcg atggcatctt
     2101 ctccgccgtg catgccgtgc gtgtgcgtaa ccagctcaag gcgtggggct ggcttctcgc
     2161 gcagggcatc ctgtccgcca tcgccggact catcgctctg atcatccccg ggctcgtggg
     2221 catcttcggt ggcttggttc tgctgtggac catcgtcatc tggaacatca cccacggggt
     2281 cgcgggcatc cgttccgccg ccggagctcc ctcgggcacc gcgaagactg tcggtatcat
     2341 cgccggggtg ttgtcgatcc tcttcggcat cctgctgggc gttctgattc tggtcacgcc
     2401 cggcgcggcc ctgctcggtc tggtctggct gatcggaatc tacgcgatcg tgttcggcat
     2461 catgctcgtc gtcgcggcga tccagtcccg cgccggttcc ggtgcgaagg ctggcgcgaa
     2521 gggcacgccg gccgcggcct agcggtgctg ctcgccctgg gtgggcgccg ccaaggtgct
     2581 gcgcttctcc cacagcaggg cttgcactcg catcgccaga tcccggagca gggcacgatc
     2641 ggtggcctcg aacgtacgcg gcttgtcatc gaccacgcac agggcgccga tgcggtgccc
     2701 gtctggtgcc tcgaggggat agcccgcgta gaaccgcacc gcatcgtcgc cgtgggccca
     2761 ctccttgtcg cggtagtcgg gattgaggag cgtgtcctcg acgacgaaca tgtccgcggc
     2821 gccgatcgtg gtggtgcaga ccgagtcccc ccggcagatg gtgccgtggc tgcgcccggc
     2881 cgacgacatc acccactggc gatcgctgtc gatgaggttg acgcctgccc cggatgcccc
     2941 gaacaggtcg cgagcggtgg cgacgatctg gtcgagttcc tcgtcccgtg gcgagtcgag
     3001 gatgcccatc tccacgagtg cggcgagccg agcatcctcg tcggtgggaa ttgtgtcgcg
     3061 atgaacccgg ccgtctagtt tgtgcgccac gctctctgcg atcgggtcag cccagcgctt
     3121 gtagagatcg cgaccgatct ggtcgagcac gcgggcctgt acgggctcga aggggatgaa
     3181 ccgagccgac ggatactcgg cgcacacctg ctcggtgatg gtattgagac gattcgtgtg
     3241 agcctcgaca gcgagaccgt agagccgcgg catccgcacc atgttgctgg acagcgggat
     3301 gccgacgaaa tgaatgctgg tgttcggcgc cgaccgcgcc aaggtctcga tgagttcgcg
     3361 caccgagtgg cgccagacat cggcggaccc gaacaacacc gcctcgagac ctccgagagt
     3421 ggagaccacg gcgtcgtact tggcgaactc gggcgacgcc agaatgggca gcgcatccgc
     3481 gggtgtgaga tcgggggccg cgcgcacatc caccgtggtg ccgcgaccgg tgagagcgga
     3541 cagacgcctc gcgaggtgac cggcgaggcc cagatcatgc gacagcacgc cgtaactgac
     3601 cgcgatgccg tttcccatca gcagcacccg atcgacgtcg gaacctgccg cgtgggccgt
     3661 ggggcgatcg gttggcacgg gaagagcgcg ccacgactca gacgatgaca tcacccaggc
     3721 gcgtgcgaag gctcgcatca gggcgtggct gagtcggtcg accatggcac tccccccgag
     3781 ctagaaactt tccccggtta cgacattacg tcgcgaaagc gcttgcggat cagaccccgc
     3841 cctgacgatg ttcgcttttc gcacgtcgct cgaacttgat gcgcgcgttc tcctcgtgca
     3901 cggccgcctg cgtcgctcgt tccaccacga gccactccgg cttctgctcg agcaactgct
     3961 tgatctccgc cgtcgtcatg ctggtgggcg cgccagcccg ggtgaggccg gacaccgaga
     4021 tgccgagctt ctgggccacg acctgcttgg ggtggggtcc ggtgcgccgc agttcaacca
     4081 gccactcggg cgggttggcc tgcaactcga gaaactgggt gcgcgtcacg agcccctccc
     4141 ggaattctgc cggggctgct tgcaggtaga tgccgagctt cttcgcggcg gtctcgggct
     4201 tcatggtctg atctggcacc ccccaaggct accggtgacg gtggcgctag cctgaagcca
     4261 tgtccgagcc cttcgtcgtt gccttcgtcg tgggggtcac gccagccaag tgggcgcgag
     4321 tctggggcga gcggatgccg cggcatccgc tcacgctgct gccgacgggt gatgctctcg
     4381 ccgccctcga cgacggaacg gcgcaggtgg ccctcactcg cctgccggtt gacgccgagc
     4441 gcttcagcgt gatcccgctc tacacggagc aggcggtcgt ggtcgcgccc aaggatcacg
     4501 cgatcgaggc gctcgacgcg ctcaccctcg ccgatctcgc cgacgagacg gtgctcgtgg
     4561 gcgagtgggc gccgaccatc gagctggtcg cagccaacgt cggggtgacg gtgcttccgc
     4621 agtctgtggc tcgggctctc agccggcgcg acgtgatcgc ccgaccggtg acggatgccc
     4681 cgaccaccca ggtcgctctg gtgtggccga tcgacgcacc gtccgatctc atcgacgagt
     4741 tcatcggcat cgtgcgtggt cgcaccgcga acagctcacg cggacaggtt gccgtgccgg
     4801 agcagaagcc cgccgcgaag ccggcggcca agccgggcgc caagtccggc gcgaaacccg
     4861 cggcacgagc gggccagaaa cctcggcggg gcgctccccc tcggccccgc aagcgatagc
     4921 ccggcggcgg tcaggtgcgg ggcagctgct gtgcgtcggc cgcgatggcc gtgaggcggc
     4981 gttgccgcat ggccaggaac agcggaaacg tgaacgcgaa cgcggtcagc ccgctcagca
     5041 cgatgtagag ccaggcgccc cgcatgccga gccgacgtga ctcgacgacg atcaggatgc
     5101 atccggcgac ggccacgaca agcaggtcga cagtgagcga cgacaccgcg ggtccgctgt
     5161 tcacccagtc ccccacgaag tcgcgcaact gcactattgc cagcgcgttg aaggtccagg
     5221 tgcccacgag tcccgcgatg gcgagcacga accaggtgat cgcaaggggt gtccagttct
     5281 tggtcatgct ccattgtgcg ctgcactggt gctgttgcgc gtgcccggtc gccgctaccg
     5341 tggtcgcatg acggatgcca cggccgaccc caccccgccc gctcccccgg tgactgcctc
     5401 gggtcggccc cctcgccgca gtgccacccc cggagtgtgg gcactggctg tcacgctgtt
     5461 tcctctgctc tacacggggg tgggtctgat tcttctggcg ttcggccagg gtgccaacgg
     5521 gatcggcggc ttcgccgagg ccgcctatgt gatgttcttc ctcgggttcc tcgtcgtgcc
     5581 tgcctgcctg atcgtcggtc tcgtgctggg catcatcgca ctcgcgttca gtcgcccgct
     5641 gggcaaggtg ctcggcggca tcgcactcgt gctcatcgtc gcgggtgtcg ttgcgctcac
     5701 gctgttgctg atgggctcga acagcccgct gttctggacc gacttctagg gttcgtgttc
     5761 ccatcgtcgg cgcttgacac gccgacgaat gtcaccggcg attgacacaa tcgaacatat
     5821 gttcgaccat ggacagatgt cctccgccct cgcagtagcg cccgcgcacg aggctgtcac
     5881 gacggcagag accgcgaaga cagccgatac cgtgcaggta gcagacaccg tgcaggcgct
     5941 tcaggagcgc atccggtcca tgcaggcgac caagctcgat tcccgactgc tgcccacgca
     6001 tcccgcgatt gctgagctgc tgcccggcgg ggggctccag cag
//
LOCUS       DAOSOU010000122         6041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13230_length_6041_cov_5.634146, whole genome shotgun sequence.
ACCESSION   DAOSOU010000122 DAOSOU010000000
VERSION     DAOSOU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13230_length_6041_cov_5.634146"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PLW15_07555"
     CDS             <1..1172
                     /locus_tag="PLW15_07555"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191392.1"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOT34283.1"
                     /translation="GQLATLLAASRAGADAVDVASAPMAGTTSQPSASALIAALAHTD
                     RDTGISLQAVSDLEPYWEAVRHVYKPFESGLPGPTGRVYKHEIPGGQLSNLRQQAIAL
                     GLGDQFEKVEDLYAAANQMLGRPPKVTPSSKVVGDLALALAAANADPADFEQNPDKYD
                     IPDSVIGFMAGELGELPGGWPEPFRSKVLKGRTVKIGVEPLGADDVTALEGDSASRRS
                     ALNRLLFAAPTKIFEASREQYGDLSVLDTVDYLYGLQQGVEHVVHIGTGVSLFVGLEA
                     IGEADDKGMRTVMSTLNGQLRPVFVRDNSITVDTKSAEKADATKPGHVAAPFSGVVTL
                     QVAEGDTVEVGQSVASIEAMKMEAAITATITGTVKRLAIPATQQVDAGDLLLVIE"
     gene            1241..>2008
                     /locus_tag="PLW15_07560"
     CDS             1241..>2008
                     /locus_tag="PLW15_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34284.1"
                     /translation="MAARSKKSDTDVLESDSGAIVVPEESVENLTFTVALPVTVHETP
                     ADEAAVAIDDVPVSPDAVDATGPTTTTITVVANDVAVHPENLVEEPHIPALPEPDQVY
                     SRRGRQRGEVSATPEPAAMLTAERLIEQGRTKRKPAPEGFLQKLLYDATLHLVNVGDS
                     MRVRQRKALDARIGKPLEGEARFIPVLTRKGGVGKTTISTLLGMAMADVREDRVIAID
                     ANPDRGTLAERVTKTTRATVRDVVTRASSIQAFSEFQT"
     assembly_gap    2009..2108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2109..2351
                     /locus_tag="PLW15_07565"
     CDS             <2109..2351
                     /locus_tag="PLW15_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOT34285.1"
                     /translation="LVKNAVVALNTSTQGTNLVKLEEIEAHFRSRVREIVRVPYDPAL
                     ATGSTIHFDELRPLTREAARELAALVVDGLPEHRDA"
     gene            2356..2847
                     /gene="def"
                     /locus_tag="PLW15_07570"
     CDS             2356..2847
                     /gene="def"
                     /locus_tag="PLW15_07570"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT34286.1"
                     /translation="MAERAIRLFGDPVLRSVCDPIRPGDARAVALVDDLLETVRLPGR
                     AGLAANQIGVALRAFSYNVDGEVGYVINPELVEVSGEPDFVEEGCLSVPGFYFPRNRY
                     PFARVRGVDLDGNPVELSGEGLMAQALQHEVDHLDGHLYIEGLAPETKKEAMRLIRMA
                     PWY"
     gene            complement(2860..4686)
                     /locus_tag="PLW15_07575"
     CDS             complement(2860..4686)
                     /locus_tag="PLW15_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT34287.1"
                     /translation="MKQFDVPALVEADPNANATDLLVARVAATPDRALFAIPTADGGW
                     RDVTAAQFQAQVIALAKGLVASGVEPGDKIGLMAKTRYEWTLIDFATWYAGAVLVPIY
                     ETSAPSQILWNLTDSGATGIITENADHFARFDEIRADVPAVRNVWQIDLGDLDKLAAS
                     GTAVKDEEIERRRSIAKGSDLATLIYTSGTTGKPKGCILTHSNFVELTRNAQKAIPEV
                     VNPDSSTLLFITLAHIFARFISVLAVHGGVKVGHQPDTKLLLPSMASFQPTFLLAVPR
                     VFEKVYNSSEQKAEAGGKGKIFRRAADVAIAHSKAVDAGHVPLGLKLQFALFDRLVLS
                     KIRAALGGRVKYAVSGSAPLGLRLGHFYRSLGLTILEGYGLTETTAPVSVNVPSNFKI
                     GKVGPPLPGVSVRIADDGEIQAKGVCVFDGYWNNPAATKEVFDGEWFKTGDIGQIDED
                     GYLEITGRKKEIIVTAGGKNVAPAALEDPIRANPIIGQVIVVGDQKPYVSALITLDEE
                     MLPAWLKNNGLDPHMPQEVAATNPAVLAEIQRAVDAANAKVSRAESIRKFRVLDADLT
                     EASGHLTPKLSIKRNVIMQDFATEVESMYAEQPATEGISLNH"
     gene            4784..5731
                     /locus_tag="PLW15_07580"
     CDS             4784..5731
                     /locus_tag="PLW15_07580"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HOT34288.1"
                     /translation="MDAIGIDIGGTKIAGAVVSEDGAILAEERVATEAGNSAAIIDAV
                     VAMVETLSQGRQIEAAGVAAPGFIDVDQSTVYYTPNISWRNEPLRDHLRARLPLDITI
                     DNDANAAGWAEYRFGAGRTVSDMTMLTIGTGVGGAIVSRGSLFRGGFGAGAELGHLRI
                     VPDGLPCGCGAHGCIEQYGSGRALLRMANEIADVGGIGLALADARREHGQLDGSIVGA
                     LIADGDPGAVHALEQLGNWLGQACASLSAVLDPELFVFGGGVAVAGELLLAPVREAYR
                     QHVPAKGYHPEPNFVIAELVNNAGVVGAADLARIHSRAR"
     gene            5777..>6041
                     /locus_tag="PLW15_07585"
     CDS             5777..>6041
                     /locus_tag="PLW15_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT34289.1"
                     /translation="MFYWFMKNLVAGPLVKTVFRPWVTGADNIPKTGAVILASNHLSV
                     VDSIFLPLVIERRISFLAKSDYFMGRGVKGWAIKNFLKGTGMLP"
BASE COUNT          973 a         1951 c         2010 g         1007 t
ORIGIN      
        1 gaggccagct ggcgacgctc ctggcggcct cgcgcgccgg tgctgacgcg gtggatgtcg
       61 cgagcgcacc gatggcgggc accacgagcc agccgtcggc atccgccctc atcgcggccc
      121 tggcgcacac cgaccgcgat accggcatct ccctgcaggc cgtctccgat ctggagccct
      181 actgggaggc cgtgcgtcac gtctacaagc cgttcgagtc cggtctgcca ggaccgacgg
      241 gccgggtgta caagcacgag atcccgggcg gccagctctc caatctgcgc caacaggcga
      301 tcgccctcgg actgggcgac cagttcgaga aggtcgagga cctctacgct gcggccaacc
      361 agatgctggg tcgaccgccg aaggtcaccc cttcctccaa ggtcgtgggc gacctggccc
      421 tggcccttgc ggcggccaac gccgatcccg cggacttcga gcagaacccc gacaagtacg
      481 acatccccga ctcggtcatc ggcttcatgg ccggcgagtt gggcgagctt ccgggtgggt
      541 ggcctgagcc gttccgcagc aaggttctca agggtcgcac cgtgaagatc ggcgtcgagc
      601 cgctcggtgc cgacgatgtg accgccctcg agggtgactc ggcgtcgcgg cgctcggcac
      661 tcaaccgtct gctcttcgcg gcccccacga agatcttcga ggcgagccgg gagcagtacg
      721 gagacctctc ggtgctcgac accgtcgact atctgtacgg cctccagcag ggcgtcgagc
      781 acgtcgtgca catcggcacc ggggtgagcc tgttcgtcgg tctcgaggcg atcggcgagg
      841 ccgacgacaa aggcatgcgc acggtcatgt cgaccctcaa cggtcaactg cgtcccgtgt
      901 tcgtgcgcga caactccatc accgtcgaca ccaagtcggc cgagaaggcg gatgccacca
      961 agcccggcca tgtcgccgcg ccgttctccg gcgtggtcac cctgcaggtc gccgagggtg
     1021 acaccgtcga ggtgggacag agcgtcgcat ccatcgaggc gatgaagatg gaagcggcga
     1081 tcacggcgac gatcacggga accgtcaagc gtctggccat ccctgcaacc cagcaggtcg
     1141 acgccgggga cctgctgctc gtcatcgaat agtccacacg cgaacgcggc tgagtgcgag
     1201 tcacgataaa ctggagtgtt tgcacgaaag gaccgtggcc gtggctgcca gaagcaagaa
     1261 gagtgatacc gacgttctcg agtcggattc cggcgcgatc gtcgtgcccg aggagtccgt
     1321 cgagaacctg actttcaccg ttgcgctacc cgtgacggtg cacgagactc cggctgacga
     1381 agcggccgtc gcgatcgacg atgtgccagt gagccccgat gctgtggatg ccacgggccc
     1441 gaccacgacc accatcaccg tcgtggcaaa cgacgtcgcc gtgcatcccg agaacctcgt
     1501 cgaggagccg cacatccccg cgctgcccga accggaccag gtctacagcc gccgcggtcg
     1561 ccagcgtgga gaggtctcgg caaccccgga acccgccgcg atgctcaccg cggagcgcct
     1621 catcgagcag gggcgcacga agcgcaagcc ggctcccgag ggattccttc agaagctcct
     1681 gtacgacgcc acactgcacc tcgtgaacgt gggcgattcg atgcgcgtgc gccagcgcaa
     1741 ggcgctcgat gcccgcatcg gcaagccgtt ggagggcgag gcgcgcttca tcccggtgct
     1801 cactcgcaag ggtggcgtcg gcaagaccac catctccacg ctgctcggca tggcgatggc
     1861 cgacgtgcgc gaggaccggg tgatcgccat cgatgcgaac cccgaccgcg gcaccctggc
     1921 cgagcgcgtg accaagacca cccgtgcgac cgtgcgcgac gtggtcacga gggcgagctc
     1981 gattcaggcg ttcagcgagt tccagaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnct ggtgaagaac gccgtcgtcg ccctgaacac ctcgacgcag ggcaccaacc
     2161 tggtcaagct ggaggagatc gaggcccact tccgttcgcg ggttcgcgag atcgtgcgcg
     2221 ttccgtacga ccccgccctc gccacgggct cgacgatcca cttcgacgag ctccgaccgc
     2281 tgacccggga ggcggcgcgg gaactcgccg cgctggtcgt cgacggcctg cccgagcacc
     2341 gggacgcctg actcgatggc agaacgagcc atccgactgt tcggcgaccc cgtgctgcgg
     2401 tctgtctgcg atccgattcg gcccggggat gcccgtgcag ttgccctcgt ggatgacctc
     2461 ctcgagaccg tacgactgcc gggccgtgcc ggcctcgccg ccaaccagat cggtgtggcc
     2521 cttcgcgcgt tcagctacaa cgtcgacggg gaggtgggct atgtgatcaa ccccgagctc
     2581 gtcgaggtgt cgggggagcc cgacttcgtc gaagaggggt gcctgtcggt gcccggcttc
     2641 tacttcccgc gcaaccgcta cccgttcgcg cgggtgcggg gcgtcgatct cgacggcaat
     2701 cctgtggaac tcagcggcga gggcttgatg gcgcaggctc ttcagcacga ggtcgaccat
     2761 cttgacggac acctctacat cgagggactc gcccccgaga cgaagaagga ggccatgcgg
     2821 ttgatccgca tggccccctg gtactagcag tcacccgcct cagtggttga gggagattcc
     2881 ctcggtcgct ggctgttccg cgtacatgct ctccacctcg gtggcgaagt cctgcatgat
     2941 gacgttgcgc ttgatgctca acttcggggt gaggtgtccg ctcgcctcgg tgaggtcggc
     3001 atcgagcaca cggaacttgc ggatggattc ggcccgcgag accttcgcgt tcgcggcatc
     3061 caccgcccgc tggatctcgg cgagcacggc cgggttggtc gctgcgacct cctgaggcat
     3121 gtgcgggtcg aggccgttgt tcttcagcca ggccggcagc atctcctcgt cgagggtgat
     3181 gagggccgac acgtagggct tctggtcgcc gacgacgatc acctgtccga tgatcgggtt
     3241 cgcgcggatg ggatcctcga gggcggcggg ggcgacgttc ttgccaccgg ccgtgacgat
     3301 gatctccttc ttgcggcccg tgatctccag gtagccgtcc tcgtcgatct gtccgatgtc
     3361 gccggtcttg aaccactcgc cgtcgaagac ctccttggtc gcggcggggt tgttccagta
     3421 cccgtcgaac acgcagacgc ccttggcctg gatctcgccg tcgtcagcga tgcgcactga
     3481 cacaccgggc aggggaggac cgaccttgcc gatcttgaag ttcgacggca cgttcaccga
     3541 caccggtgcg gtggtttcgg tcaggccgta gccctccagg atcgtgagtc ccaggctgcg
     3601 gtagaagtgg cccaggcgta gtccgagggg cgccgagccc gacaccgcgt acttgacgcg
     3661 accgcccagc gccgcgcgaa tcttgctcag cacgagacgg tcgaacaggg cgaactgcag
     3721 cttgagcccc agcgggacgt gtccggcgtc gacggccttc gagtgagcga tcgcgacgtc
     3781 ggccgcgcgg cggaagatct tgcccttgcc gccggcttcg gccttctgct cggacgagtt
     3841 gtagaccttc tcgaacacgc gcggcacggc caacaggaac gtcggctgga acgacgccat
     3901 ggacggcagg agcagtttcg tgtcgggctg gtgtcccacc ttgacgccgc catgtactgc
     3961 aagcacggag atgaaccggg cgaagatgtg cgcgagggtg atgaacagca gcgtgctcga
     4021 gtccgggttc acgacctcgg ggatcgcctt ctgcgcgtta cgggtgagct cgacgaagtt
     4081 cgagtgggtg aggatgcagc ccttcggctt gccggtcgtg cccgacgtgt agatgagagt
     4141 tgcgaggtcg ctgcccttcg cgatcgagcg gcggcgctcg atctcctcgt ccttgacggc
     4201 ggtgcccgag gcggcgagct tgtccaggtc gccgaggtcg atctgccaga cattgcgcac
     4261 ggccggaacg tcggcgcgga tctcgtcgaa tcgagcgaag tggtcggcgt tctcggtgat
     4321 gatcccggtc gcgcccgagt cggtgaggtt ccagaggatc tgggaggggg ccgacgtctc
     4381 gtagatcggc accagcacgg cgccggcgta ccaggtggcg aagtcgatca gcgtccattc
     4441 gtagcgagtc ttcgccatca gaccgatctt gtcgccgggc tccacaccgg aggcgacgag
     4501 acccttggcg agggcgatca cctgcgcctg gaactgggct gccgtcacat cccgccagcc
     4561 gccgtcggct gtggggatcg cgaagagcgc acgatcgggg gtggcagcga ccctggcaac
     4621 gagcaggtcg gtggcgttgg cattcggatc cgcctcaacg agggcgggta cgtcgaactg
     4681 tttcacggca actcctttgg taccggtcgg ggtcattcgt tctactctaa ccgggcaggc
     4741 tgagggaaac cacgctgtcg cgccgccggt acactcgatc accgtggatg ccattgggat
     4801 tgatatcggg gggaccaaga tcgccggcgc ggtggtttcc gaggatggcg cgatcctcgc
     4861 ggaggagcga gtagcgaccg aggcgggcaa ttctgcggcc atcatcgatg ccgtcgtggc
     4921 catggtcgag acgttgtcgc aggggcgtca gatcgaggcg gcgggagtcg ccgcacccgg
     4981 attcatcgac gtcgatcagt ccaccgtgta ctacacgccc aacatcagct ggcgaaacga
     5041 accgcttcgg gatcacctgc gcgctcgtct gccgctcgac atcaccatcg acaacgacgc
     5101 caacgccgcc ggctgggcgg agtaccggtt cggtgcgggg cgcaccgtca gcgacatgac
     5161 gatgctcacg atcggcactg gcgtgggcgg ggcgatcgtc agccgcggct ccctgttccg
     5221 gggtggattc ggcgccggtg ccgagctcgg ccatctgcgc atcgttcccg acggtctgcc
     5281 ctgcggatgc ggcgcgcacg gatgcatcga acagtacggt tcgggtcgtg ccctgctgcg
     5341 catggcgaac gagatcgctg acgtcggcgg gataggcctc gccctggcag acgcccggcg
     5401 tgagcatgga cagctcgatg gttcgatcgt cggcgcactc atcgccgacg gcgaccccgg
     5461 ggccgtgcac gcgctcgagc aactcggcaa ttggctcggc caggcctgcg cctcgctgag
     5521 cgcggtgctc gatcccgagc tcttcgtgtt cggaggtggc gttgctgtgg cgggggagtt
     5581 gctcctggcg ccggtgcgcg aggcgtaccg tcagcacgtg cccgccaagg ggtatcaccc
     5641 cgagccgaac ttcgtcatcg ccgagctcgt caacaatgcc ggagtcgtcg gtgccgccga
     5701 tctggcccgc attcactcgc gtgcgcgcta gagtcggcgc gctagagtgg aagctcccgg
     5761 ccggaggtag gtagcgatgt tctactggtt catgaagaac ctggtcgccg gcccattggt
     5821 caagaccgtg ttccggccgt gggtgaccgg cgccgacaac atcccgaaga ccggtgccgt
     5881 cattctcgcg agcaaccacc tctcggttgt cgactcgatc ttcctcccgc tggtgatcga
     5941 gcgacgcatc tccttcctcg ccaagagtga ctacttcatg ggccgcgggg tgaagggctg
     6001 ggcgatcaag aacttcctca agggaacggg catgctgccc a
//
LOCUS       DAOSOU010000123         6029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13266_length_6029_cov_5.480750, whole genome shotgun sequence.
ACCESSION   DAOSOU010000123 DAOSOU010000000
VERSION     DAOSOU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13266_length_6029_cov_5.480750"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLW15_07590"
     CDS             <1..881
                     /locus_tag="PLW15_07590"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT34290.1"
                     /translation="HRLKSRSAREECVVSASAVLVTGASTGIGRATALALDARGITVF
                     AGVRKPADGDALVAAGRHIVPLILDVTVAEQIAVAARAIAESGIPLVGLVNNAGIAAA
                     APLEFIAVDDFRHQLEVNVVGQLAVTQSVLPLLRASKGRIVNITSIGGLISGPMLGPY
                     HASKFALEAMTDTLRIELAPWGIGVAAIEPGQIATPIWATASAKADTMIDGMPPEAAE
                     FYAKGIAGARASAKRAAAEGLPPERVADAIIHALTAARPKTRYLVGSDAKLIGRVVRH
                     LPDRLRDRMMVRRRSH"
     gene            complement(912..1166)
                     /locus_tag="PLW15_07595"
     CDS             complement(912..1166)
                     /locus_tag="PLW15_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HOT34291.1"
                     /translation="MTHLDGNALAGPLSELFRDDMTTALGRCAGCGDVSVLAEALVYD
                     AAPGWAVRCHACGDVLMTFVRTLDRLRIDMRGLTALEIPA"
     gene            complement(1166..1888)
                     /locus_tag="PLW15_07600"
     CDS             complement(1166..1888)
                     /locus_tag="PLW15_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT34292.1"
                     /translation="MTFRDWLVGTVVSTTMQTPTAKSIVLDVPGWKGSTAGQHVDVRL
                     TAEDGYQAVRAYSLASTGPSDRVELAVDLIEDGEVSPYLVGELREGDQLEFRGPLGRW
                     FVWKPGDTQPVQLVAGGSGVVPLLAMARAHAASASEAKFRLLYSVRGPAEVFYADELA
                     SLAGPNFEVTFVYTRETPPGWPHPAGRVSRDILGSATIPAAENPAVFVCGPTGFVESV
                     ADWLVDLGHNALAVKTERFGGN"
     gene            complement(1896..2489)
                     /locus_tag="PLW15_07605"
     CDS             complement(1896..2489)
                     /locus_tag="PLW15_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT34293.1"
                     /translation="MAIFSRGFSGRPRESNPDLPPGQFLTEDFPVLSAGPTPRLDTAD
                     WKFEIKTETGQTLSWNWDELNALGLEDVHTDIHCVTRWSKLGTDWRGVSLDKLFEDAE
                     TSFDFVMVHSYGGYTTNLPLEDLMNGQAWIATEFGGEPLEPDHGGPARLLVPHLYFWK
                     SAKWVRGITMQRTDSPGFWEQNGYHIYGDPWREERYW"
     gene            2593..3690
                     /gene="moaA"
                     /locus_tag="PLW15_07610"
     CDS             2593..3690
                     /gene="moaA"
                     /locus_tag="PLW15_07610"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOT34294.1"
                     /translation="MMTLSLGLPGIRRDPGVPPSTDGRPDVSGLIDRFGREAHDLRVS
                     ITEKCSLRCTYCMPSEGLPAIARDQLLTAQEIARLVGIAVRDLGVRDIRFTGGEPLMR
                     ADLEEIVGLSAGEISTSAITGVSLSMTTNGIGLDKRVDGLVAAGLNRINISLDTVDRD
                     HFARLTRRDRLPAVFAGIDAARRAGITPVKINAVLMRDTLAGAADLLEWALGNGARLR
                     FIEQMPLDADETWVRDNMVDAAELLDVLGSRFTLSSPHRDDPSAPAEEWLVEGYQRDG
                     GPATVGIIASVTRSFCEACDRTRLTAEGTVRSCLFGDDETDLRALLRGGADDAAIADR
                     WRAAQWQKQQGHGISRPDFVRPLRSMGAIGG"
     gene            3683..3928
                     /locus_tag="PLW15_07615"
     CDS             3683..3928
                     /locus_tag="PLW15_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOT34295.1"
                     /translation="MAEGVLVRYFAAAADAAGCEEELLPAVSTVRELKSVLVERYGDA
                     MARVLASGSFLVDGVVSRDDSRALGARVDVLPPFAGG"
     gene            complement(4140..4586)
                     /locus_tag="PLW15_07620"
     CDS             complement(4140..4586)
                     /locus_tag="PLW15_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HOT34296.1"
                     /translation="MPRKAAPSQGTMNSMNRIHRALITLSGGRLGWRMAGMPMLELTT
                     TGRKSGELRSTMLSSPLQPHGSYVVVASRGGDDRHPAWYLNLVENPEVQVVFQGGARE
                     VRHARVATEIERARLWPIITDDHKNYAGYQEKTDREIPLVFLDPIR"
     gene            complement(4632..5132)
                     /locus_tag="PLW15_07625"
     CDS             complement(4632..5132)
                     /locus_tag="PLW15_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOT34297.1"
                     /translation="MSEADGPDLDTARSPVAKPVEILAASVIEPVEILAAISDTPLDA
                     AALEHFVLTPANGALVTFRGVVRDHDHGLAVSALDYQAHPDAEGFLVDCCRQVSEATG
                     LRVAAAHRVGALVIGDVALVASVAAPHRAEAFAACEQLVDLIKLTVPIWKRQHLADGA
                     TEWVGL"
     gene            complement(5125..>6029)
                     /gene="moaCB"
                     /locus_tag="PLW15_07630"
     CDS             complement(5125..>6029)
                     /gene="moaCB"
                     /locus_tag="PLW15_07630"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HOT34298.1"
                     /translation="RARMVDVGDKAVTRRSATARGRFETTQEVLALVRADGLPKADVL
                     ATARIAGIQGAKRTSELIPLCHPIALTSVSVDFTLNDDWIEIVATAKTTGKTGVEMEA
                     LTAVAIAGLTLHDMTKAVDPQSVLGEIQLIEKTGGRTRPEVVPGTAAVVVASTRAAAG
                     TRDDTTGPVIATWLAERGYSASVTVVADADVASALDPIIAAGPAVVITTGGTGMSPTD
                     RTPEATAALLDRELPGIPEAIRARGLAHTPKAALSRGVAGTVGGTVIVNLPGSTGGVK
                     DGLAVLDELLEHLVAQVAGGGDHE"
BASE COUNT          867 a         2029 c         2188 g          945 t
ORIGIN      
        1 gccatcgcct caagtcgcgg tcggcgcgcg aggagtgcgt cgtgagcgcc tcagcagttc
       61 tcgtgaccgg ggcatccacc ggcatcggtc gcgccaccgc cctggctctg gatgcccgtg
      121 gcatcaccgt cttcgcgggg gtgcgcaagc cggccgacgg tgacgcgctg gttgcggccg
      181 ggcggcacat cgtgccgctc atcctcgacg tcacggtcgc cgagcagatc gcggtcgcag
      241 cgcgggcgat agccgagtcg ggcatccccc tggtcggact cgtcaacaac gccggcatcg
      301 ccgccgccgc gccgctcgag ttcatcgcgg ttgacgactt ccgccaccag ctcgaggtca
      361 acgtcgtggg ccagctcgcc gtcacgcagt cggtgcttcc gctgctgcgg gcgtcgaaag
      421 ggcgcatcgt caacatcacg tcgatcggcg ggctgatctc ggggccgatg ctcggtccgt
      481 accacgcgtc gaagttcgcg ctcgaggcga tgaccgacac cctgcgcatc gagctggcac
      541 cctggggcat cggggtagcg gcgatcgagc ccggacagat cgccacaccg atctgggcca
      601 ccgcatcggc caaggcggac acgatgatcg acggcatgcc gcccgaggcg gcggagttct
      661 acgccaaggg gatcgccgga gcccgcgcct cggcaaagcg cgccgccgcc gaaggtctgc
      721 cgcccgagcg ggtcgccgac gcgatcatcc acgcgctcac cgcagcgcgc ccgaagaccc
      781 gctacctggt cggctcggac gccaagctca tagggcgggt cgtgcgtcac ctgcccgatc
      841 gcctccgcga ccggatgatg gtgcgccggc gttcacactg agggaggcgc ccgcggtgcc
      901 gggcgcaggc gtcaggcggg gatctccagc gccgtaaggc cccgcatgtc gatgcgcagg
      961 cggtcgagcg tgcgaacgaa ggtcatgagc acgtcgccgc aggcgtgaca tcgcaccgcc
     1021 cagccggggg ctgcgtcgta gaccagcgcc tccgcgagca cgctcacgtc gccgcagccc
     1081 gcgcagcgcc cgagggccgt ggtcatgtcg tcgcggaaca gttccgacag aggcccggcg
     1141 agggcgttgc catcgagatg ggtcatcagt tgcctccgaa tcgctcggtc ttcacggcga
     1201 gggcgttgtg cccgagatcg accagccaat cggcgacgct ctcgacgaag ccggtcgggc
     1261 cgcagacgaa gacggcgggg ttctcggcgg cagggatggt ggccgacccg aggatgtcgc
     1321 gactgacccg accggcgggg tggggccatc cgggcggggt ctctcgggtg tagacgaagg
     1381 tgacctcgaa gttcgggcca gcgagcgagg cgagctcgtc ggcgtagaac acctcggcgg
     1441 ggccgcgcac cgagtacagc agccggaact tcgcttcaga ggcggatgcc gcatgcgcac
     1501 gcgccatcgc cagtaggggc accacgcccg aacctcccgc gacaagctgc acgggctggg
     1561 tgtcgcccgg cttccagacg aaccagcgac cgagcggccc gcggaattcg agctggtcgc
     1621 cctcccgcag ctcgccgacg aggtaggggg agacctcgcc gtcttcgatg aggtcgacgg
     1681 ccagttcgac tcggtcgctc gggccggtgg aagccagcga gtaggcgcgc acggcctggt
     1741 aaccgtcctc ggcggtcagc cgcacgtcga cgtgctgacc ggcagtgctg cctttccagc
     1801 cgggcacgtc gagcacgatg ctcttggcgg tcggggtctg catggtcgtg gacacgacgg
     1861 tgcccacgag ccagtcacgg aaggtcaccg gtgtgctacc agtaccgttc ctcgcgccac
     1921 gggtcaccgt agatgtggta cccgttctgc tcccagaagc cggggctgtc ggtgcgctgc
     1981 atggtgatgc cgcgcaccca cttggcgctc ttccagaagt agaggtgcgg tacgagcagc
     2041 cgcgcgggac cgccgtggtc gggttcgagg ggctccccgc cgaactcggt cgcgatccag
     2101 gcctgaccgt tcatcaggtc ttcgagcggg aggttggtcg tgtagccgcc gtaggagtgc
     2161 accatcacga agtcgaagct cgtctcggca tcctcgaaga gcttgtcgag cgagaccccg
     2221 cgccagtcgg tgccgagctt cgaccagcgg gtgacgcaat ggatgtcggt gtgcacgtct
     2281 tcgaggccga gggcgttgag ctcgtcccag ttccacgaga gcgtctgccc ggtctcggtc
     2341 ttgatctcga acttccagtc cgccgtgtcg aggcgcgggg tgggtccggc ggagagcacc
     2401 gggaagtcct cggtgaggaa ctggcccggc gggaggtccg ggttcgactc gcgggggcgg
     2461 ccggagaagc cgcgggagaa gatggccata gctcagcgta taaggccggt tgagtaggcc
     2521 ataggccgta tcgaaacctc cgcggtgcgg tttcgatacg cgcttcgcgc tactcaaccc
     2581 gcttagggtt ggatcatgac gctctcgctc gggttgcccg gcatccgccg cgatcccggc
     2641 gtgccgccgt cgaccgacgg gcgaccggat gtctcgggcc tcatcgatcg cttcggccgc
     2701 gaggcccacg acctgcgggt ctccatcacg gagaagtgct ccctgcgctg cacgtactgc
     2761 atgccttcgg agggtctgcc cgcgattgcc cgggaccagc tgctgacggc gcaggagatc
     2821 gcgcgcctgg tcggcatcgc cgtgcgcgac ctgggggtgc gcgacatccg cttcaccggg
     2881 ggtgagccgc tcatgcgcgc agacctcgag gagatcgtgg ggttgagcgc gggggagatc
     2941 tcgacaagcg cgatcaccgg ggtttcgctc agcatgacga ctaacggcat cggcctcgac
     3001 aagcgggtcg acggtctggt cgccgcggga ctgaaccgca tcaacatctc gctcgatacc
     3061 gtcgatcgcg accacttcgc gcggctcacc cgccgcgatc gtctgccggc ggtgttcgcg
     3121 ggcatcgacg ccgcccggcg agccggcatc accccggtga agatcaacgc cgtgctcatg
     3181 cgcgacaccc tcgctggcgc cgccgacctg ctcgagtggg ctctcggcaa cggcgcgcgg
     3241 ttgcgcttca tcgagcagat gcccctcgac gccgacgaga cgtgggtgcg cgacaacatg
     3301 gtcgacgctg ccgagctgct cgacgtgctc ggctcgcgct tcacgctgtc gtcgccgcac
     3361 cgcgacgacc cgtcggcgcc cgcggaggag tggttggtcg agggctacca acgcgatggc
     3421 ggccctgcga cagtgggcat catcgcctcg gtgacccgca gcttctgcga ggcctgcgac
     3481 cgcacccgcc tgaccgccga gggaacggtg cgctcgtgtc tgttcggcga cgacgagacc
     3541 gacctccgcg ccctgctccg tggcggtgcg gatgacgcgg cgatcgccga ccggtggcga
     3601 gccgcccagt ggcagaagca gcagggccac ggcatctcgc ggccggattt cgtgcgaccg
     3661 ctgcgctcga tgggggcgat cggtggctga aggtgtgctc gtgcggtact tcgcggcggc
     3721 cgcggacgcc gcggggtgcg aggaggagct tctgcccgcg gtgtccaccg tgcgggagtt
     3781 gaagtcggtg ctcgtggagc ggtacggcga cgccatggcg cgggtgctgg catccggatc
     3841 gttcctcgtc gacggggtcg tcagtcgtga cgattcgcgc gcgctcggcg cccgggtcga
     3901 cgtgctgccg ccgttcgcgg gcggctagcc ccggcgcttt cgacgcgact gtgtggtgcg
     3961 ggatgcttct gttatgcagg aggcctgagg catccgcatg tctcctgcac aaccggggac
     4021 ctgagccgag cgcgcgtttc ctgcagaaca gaaggtcgca gcgggccagc gtcgcgctgc
     4081 ggtctgggcc cggttgaggt ggtgggccgc ctcattgcga gcgcgagtga aggctcggtc
     4141 tagcggatcg ggtcgaggaa gacgagcggg atctcccggt cggtcttctc ctggtagccg
     4201 gcgtagttct tgtgatcgtc ggtgatgatc ggccacagcc gggcgcgctc gatctcggtc
     4261 gcgacacgtg cgtgccgcac ctcgcgcgcg ccgccctgga acacgacctg cacctcgggg
     4321 ttctcgacga ggttgaggta ccacgcgggg tggcggtcgt ccccgccgcg ggaggcgacg
     4381 accacgtagc tgccgtgggg ctgcaacggg gagctcagca tcgtcgaccg gagctcgccc
     4441 gacttgcggc ctgtcgtggt cagctcgagc atgggcatcc cggccatgcg ccaccccagt
     4501 cgaccgcccg agagcgtgat cagcgcgcgg tggatccggt tcatcgagtt catcgtgccc
     4561 tgtgagggcg ctgcttttct tggcacctca caacgctacc cgcgctgcgg cgtcgctcag
     4621 accacccggg cctagagccc gacccactcc gttgccccgt cggcgaggtg ctggcgcttc
     4681 cagatcggca ccgtcagctt gatgagatcg accagctgct cgcacgccgc gaacgcctcg
     4741 gcgcgatgcg gtgcggcgac ggatgccacg agcgcaacat ccccgatgac cagggcaccg
     4801 acgcgatgcg ccgcagccac tcgcagcccg gtcgcctcgg acacctggcg acagcagtcg
     4861 acgaggaagc cctcggcgtc ggggtgggcc tgatagtcga gcgccgacac cgcgagtccg
     4921 tggtcgtggt cacgcacaac tccgcgaaag gtgacgagag cgccgttggc gggggtcagg
     4981 acgaagtgct cgagggccgc ggcatccagc ggggtgtcgg agatcgcggc gaggatctcg
     5041 acgggctcga tcaccgatgc cgcgaggatc tcgacgggct ttgccaccgg tgatcgagct
     5101 gtgtcgagat cgggcccgtc tgcctcactc atggtcgccg ccacccgcga cctgggcgac
     5161 gaggtgctcg agcagctcat cgagcacggc gaggccgtcc ttcacgccgc cggtcgagcc
     5221 gggcaggttg acgatgacgg tgccaccgac agtgccagcg acaccacggc tgagcgcggc
     5281 cttcggggtg tgggcgagcc cgcgggcgcg gatcgcctcg gggatccccg gtagctcgcg
     5341 gtcgagcagg gccgcggtcg cctccggcgt gcggtccgtg gggctcatgc cggtgcctcc
     5401 ggtggtgatc acgacggccg ggcctgccgc gatgatcgga tcgagggcgg atgcgacatc
     5461 cgcgtcggcg acgacggtga cactcgcgct gtagccgcgc tccgccagcc aggtggcaat
     5521 caccgggccg gtcgtgtcgt cgcgagtgcc ggccgccgcg cgggtggagg cgacgaccac
     5581 ggccgcggtg ccgggcacga cctccggtcg ggtgcgacct ccggtcttct cgatgagctg
     5641 gatctcgccg agtaccgact gcgggtcgac ggctttggtc atgtcgtgca gggtgaggcc
     5701 agcgatggcg actgcggtga gcgcctccat ctcgacgccg gtcttgccgg tcgtcttcgc
     5761 ggtcgcgacg atctcgatcc agtcgtcgtt gagagtgaag tcgacgctca ccgacgtgag
     5821 cgcgatcggg tggcacagcg ggatcagctc gctcgtgcgc ttggcgccct gaatgccggc
     5881 gatgcgtgcg gtcgccagca cgtcggcctt cggcaggcca tcggcacgca cgagcgcgag
     5941 cacctcctgc gtggtctcga agcgtccgcg cgctgtggcg ctgcgacgcg tgacggcctt
     6001 gtcgccgacg tcgaccatgc gtgcgcgcc
//
LOCUS       DAOSOU010000124         5924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13579_length_5924_cov_5.292384, whole genome shotgun sequence.
ACCESSION   DAOSOU010000124 DAOSOU010000000
VERSION     DAOSOU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13579_length_5924_cov_5.292384"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLW15_07635"
     CDS             complement(<1..578)
                     /locus_tag="PLW15_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOT34299.1"
                     /translation="MPGENLTRIEAQERAALVSTKSYEIELDLTTGDEVFRSTTRVLF
                     SATPGASTFIDALTRTVHSVTLNGTEIEHGDNVRIPLPGLAAENELVVVADFEYTNTG
                     EGLHRFVDPVDGEVYLYSQFEVPDSRRVFAVFEQPDLKATFQFTVTAPARWEVVSNSP
                     TPAPVPASTSSTTEAATWSFAPTPILSSYVTAL"
     gene            complement(677..1312)
                     /locus_tag="PLW15_07640"
     CDS             complement(677..1312)
                     /locus_tag="PLW15_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34300.1"
                     /translation="MNSFPSVMPILSAGRHRNPRQGACFMEFASYLAGERWSDHPACT
                     HPLLASLARDVNDLTSNSARSSLLHLIPRVVGLTSNDPLVTATIAMRAGAAAYPIASL
                     ERQRAIAAGMLNVLATIDASALRLIAEGAFDQAPDARRWAQRFLDRGSLRIDFGIRAA
                     AAMVNTSTIGIALACVPDPDARLANLLEQAIADTEFLVRGSTRQTADLVTA"
     gene            1412..1993
                     /locus_tag="PLW15_07645"
     CDS             1412..1993
                     /locus_tag="PLW15_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34301.1"
                     /translation="MNQQELFLLADATLRSVIDQLTPEQLASPAPTAWSRTDGATLRD
                     ILASHAKDEAWVPDVVAGRTIEEVGDRWEGDLLGDDPIGSYDRLNDAATAALAHPLHL
                     EHVVHLSYGDFPMSTYFEHTTFYRTFQAWDIAKLVGLDATLPPELVDGVWELVEVQVE
                     GLRAIHVFGPEIPVPAGADAQTRLLCVTGFWHE"
     gene            2014..2502
                     /locus_tag="PLW15_07650"
     CDS             2014..2502
                     /locus_tag="PLW15_07650"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HOT34302.1"
                     /translation="MRIHIATDHAGLEFSEDLQSHLRAAGHEVIDHGPTSYDPLDDYP
                     SFCINAAIGVVTDQAAGIECLGIVFGGSGNGEQIAANKVKGARAALVWSEATALLARQ
                     HNDANLISIGARQHTVEEAKHFIDVFIAEPFSGEERHARRIAQLAEFESTGLIAGHEI
                     GV"
     gene            2502..3473
                     /locus_tag="PLW15_07655"
     CDS             2502..3473
                     /locus_tag="PLW15_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOT34303.1"
                     /translation="MPEGHSVHRIARQFALHFVGRRVAASSPQGRFAAGAAQLDGHVM
                     TEAMAVGKQMFLEFDNGLWLRVHLGIYGAWDFSGDITADATIASSNGRIGQTNQRGTV
                     VGEHEDSLSSIGAPRRTRLRMAEQERLETPADTFPPEPIGAVRVRLLTDATCADLRGP
                     TACDVLTPDEVQAVIAKLGPDPLVDTGRAAEDRFTATVLKKPTPICLLLMDQSVVSGI
                     GNVYRAEILFRARLNPHIPGRDLPEATVRALWKDWVKLLKIGVKNGQMMTMDGLSKSA
                     YDGAMASRADRHWVYHRTGLPCRVCGTSILMEEIGGRKLYWCPVCQA"
     gene            complement(3492..3893)
                     /locus_tag="PLW15_07660"
     CDS             complement(3492..3893)
                     /locus_tag="PLW15_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT34304.1"
                     /translation="MRYLLDTNIVIFALRTRAEALRARFAAATDQMAVSTITVAELAY
                     GVERSSRPDQNRGAVEEFLALLEVMPLDASAAEHAGRIRAELAITGTPIGAYDALIAG
                     QARSRGLVVVTNNTREFARVTGLLLEDWGRE"
     gene            complement(3896..4129)
                     /gene="vapB"
                     /locus_tag="PLW15_07665"
     CDS             complement(3896..4129)
                     /gene="vapB"
                     /locus_tag="PLW15_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOT34305.1"
                     /translation="MTRTTIFKNNRTQSVRLAKEVAFPESVREVDVVVVGSSRLVSPR
                     GRTWEHWFAHGPTVTEDFLVDRDEPLPEDRGTL"
     gene            4181..4801
                     /locus_tag="PLW15_07670"
     CDS             4181..4801
                     /locus_tag="PLW15_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOT34306.1"
                     /translation="MRHTPGYLLTDPDEVKRLIRENPWATIVSHTAAGLVASHYPVIL
                     EEDAEGISIVSHVGRPDEKLHELGEHEVLVIIQGPHGYISPGWYDEGDFVPTWNHVTA
                     HLYGTPELLSDEENFTVLGRLVDTFEDRMPEPVHLDVDEDAARRIARGTVGLRLRVTR
                     FDARLKLSQNKSPEVVDRIITELQAGEQYSSPGLAREMRRVHHDPS"
     gene            4788..>5924
                     /locus_tag="PLW15_07675"
     CDS             4788..>5924
                     /locus_tag="PLW15_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT34307.1"
                     /translation="MILRNGRLGGDTVDLRLVDGVIVEIGTIDEPGLDLGGRWVIPGL
                     WDQHVHFSQWAMSRQRVDVSSATSAAEAAALAAAAPRPPAGLVLVGVGYRDGLWPDEP
                     SLEVLDAAIPDVPVVLISGDLHSCWLNSAAATRFGVTGPLLREEDCFAVVAALDDVPT
                     EVLDGWVADAGRAAAARGVVGVVDLEMTWNIDNWLRRMAAGFDTLRVRSGIYPQFLDR
                     AIAAGYRTGTELGPLLHVGPFKIITDGSLNTRTAFCVDPYPGMGDYRGLLTVQPHDLV
                     EWLARAEAFTPAVHAIGDAANHLALDAFESLGVTGRIEHAQLVSDDDFARFAELGVIA
                     SVQPEHTMDDRDVAERYWAGRTGRGYALASLHDAGATLLLGSDAP"
BASE COUNT          961 a         2013 c         1998 g          952 t
ORIGIN      
        1 agggcggtca cgtagctcga aaggatcggg gtcggtgcga agctccacgt ggcggcttcg
       61 gtggtcgagc tggtcgaggc cggtaccggc gccggcgtcg gcgagttgct cacgacctcc
      121 caccgcgcgg gagcggtcac cgtgaactgg aaggtggcct tgaggtcggg ctgctcgaac
      181 accgcgaaga cgcgacggga gtcgggaacc tcgaactggg agtacaggta gacctcgccg
      241 tcgaccgggt cgacgaaacg gtgcagcccc tcgccggtgt tcgtgtactc gaagtcggcg
      301 acgacgacga gctcgttctc ggccgcgagg ccgggcaggg ggatgcgcac gttgtcgccg
      361 tgctcgatct cggtgccgtt cagggtgacc gagtgcacgg tgcgcgtgag cgcgtcaatg
      421 aaggttgagg ctccgggggt cgctgagaag agcacgcgag ttgtcgagcg gaacacctcg
      481 tcaccggtgg tgaggtccaa ctcgatctcg taggacttcg tgctgacgag cgcagcgcgc
      541 tcctgggcct cgatgcgggt gaggttctct cctggcacgt gcttgctccc tgttcgttcg
      601 ttgcgcgcgg ctcgtggccg catcgaacaa gcttaggaga cagccgctgg aagcaggggg
      661 tggggcgcgg tgaggatcac gcagtgacga ggtccgccgt ctgccgcgtg ctgccccgca
      721 ccaggaactc ggtgtcggca atggcctgtt cgaggaggtt cgcgagccgg gcatccgggt
      781 cggggacaca ggccaacgcg atgccgatcg tcgaggtatt gaccatggcg gcggccgcgc
      841 ggatgccgaa gtcgatgcgc aggctgcccc ggtcgaggaa gcgctgagcc cagcgcctgg
      901 cgtcgggagc ctgatcgaac gcgccctcgg caatcagtcg tagcgcggag gcgtcgatgg
      961 tcgccagcac gttgagcatt ccggcggcga tcgcccgctg ccgctcgaga ctcgcgatcg
     1021 ggtaggccgc ggcaccggcg cgcatggcga tcgtcgccgt gaccagcgga tcgttcgacg
     1081 tgagccccac cacgcgcggt atgaggtgaa gcagcgacga ccgtgccgag ttgctcgtca
     1141 ggtcgttgac gtcgcgggcg agagaggcga gcagcggatg ggtgcaggcc gggtgatcac
     1201 tccaccgttc gcccgcaaga tacgacgcga actccatgaa acacgcaccc tggcgagggt
     1261 tgcggtgtcg acccgccgac aggatcggca tgactgaagg aaacgagttc atggtgccct
     1321 cctcacatca ggtgtctgtg caagtctgct cctcaccggc accaaagacc agactctgag
     1381 gggcgtcaga ggccgggtgt accgtggccg catgaaccag caggaactct tccttctcgc
     1441 ggatgccacg ctccgctcgg tcatcgacca gctcacgccc gagcagctcg cctcgcccgc
     1501 ccccaccgct tggagtcgca ccgatggtgc gaccctgcgc gatatcctcg cctcgcacgc
     1561 caaagacgaa gcgtgggtgc ccgacgtcgt cgcgggtcgc accatcgagg aggtcggcga
     1621 ccgctgggag ggtgaccttc tgggtgacga cccgatcggc agctacgacc gactcaacga
     1681 cgcggccact gccgcgctcg ctcacccgct ccacctggag cacgtggttc acctcagcta
     1741 tggcgacttc ccgatgtcga cttacttcga gcacacgacc ttctaccgca ccttccaggc
     1801 gtgggacatc gcgaagctcg tcggcctcga cgccacgctg ccgcccgaac tcgtcgacgg
     1861 ggtgtgggag ctcgtggagg tgcaggtcga ggggttgcgc gcgatccacg tgttcggacc
     1921 cgagatcccg gttccggccg gcgccgacgc ccagacccgg ctgctgtgcg tgacgggatt
     1981 ctggcacgag taggcgctcc taaactggtc agcatgcgca tccacatcgc gaccgaccac
     2041 gccggcctcg agttcagtga ggacctccag tcgcacctgc gggctgccgg tcacgaggtc
     2101 atcgaccacg gaccgacgag ctacgacccg ctcgacgact acccgagctt ctgcatcaat
     2161 gcggccatcg gcgtcgtgac cgaccaggcc gcgggcatcg agtgcctcgg catcgtgttc
     2221 ggcggatcgg gcaacggtga gcagatcgcc gccaacaagg tgaagggtgc gcgtgctgcg
     2281 ctcgtgtgga gcgaggcgac cgcgcttctc gcccgccagc acaacgacgc caacctcatc
     2341 tcgatcggtg cgcgccagca cacggtcgag gaggccaagc atttcatcga cgtcttcatc
     2401 gccgaaccgt tctccggtga ggagcgccac gcgcgtcgca tcgcccagct cgccgagttc
     2461 gagtccacgg gcctgatcgc gggtcacgag atcggggtct agtgcccgag ggtcactccg
     2521 tccatcggat cgcgcgccag ttcgcgctgc acttcgtggg gcgtcgggtc gcggcatcct
     2581 caccgcaggg gcgtttcgcg gccggcgccg cccagctcga cggtcacgtc atgaccgagg
     2641 cgatggccgt gggcaagcag atgttcctcg agttcgataa cgggctgtgg ctcagggtgc
     2701 acctgggcat ctacggcgcg tgggactttt ccggcgatat cacagcggat gccacgatcg
     2761 cctcgtcgaa cggacgcatc ggccagacca accagcgcgg caccgtcgtc ggggagcacg
     2821 aggattcgct ctccagcatc ggcgctccgc gccgaacccg actgcggatg gccgaacagg
     2881 agcgcctcga gacgcccgca gacacgtttc ccccggagcc gatcggcgcg gtgcgcgttc
     2941 gcctgctgac ggatgccacc tgcgccgacc tgcgcggacc caccgcgtgc gacgtgctca
     3001 cccccgacga ggtgcaggcc gtcatcgcca agctcggacc cgaccccctc gtcgacacgg
     3061 gacgagccgc ggaggaccgc ttcaccgcga ccgtgctgaa gaagcccacg ccgatctgcc
     3121 tgctgctcat ggaccagtcg gtggtcagtg gcatcggcaa cgtctatcgt gccgagatac
     3181 tgttccgggc gcggctgaac ccgcacatcc ccggtcgtga cctgcccgag gccaccgtgc
     3241 gtgcgctgtg gaaggactgg gtgaagcttc tgaagatcgg cgtgaagaac ggccagatga
     3301 tgacgatgga tggcctcagc aagtccgcgt acgacggcgc catggccagc cgcgccgacc
     3361 ggcattgggt gtaccaccgc accggtctgc cctgccgggt ctgcggcacc tcgatcctca
     3421 tggaggagat cggtggtcgc aagctctact ggtgccccgt ctgtcaggcc tgaggtagtt
     3481 gccggtagct ctcactcccg cccccagtcc tcgaggagca agcccgtgac ccgcgcgaac
     3541 tcccgagtgt tgttcgtgac gaccacgagt ccccgtgatc gggcctgccc cgcgatcagg
     3601 gcgtcgtacg cgccgatcgg tgtgcccgtg atcgcgagct cggcacggat gcgcccggcg
     3661 tgttcggcgg ccgaggcatc cagcggcatc acctcgagca gggcgaggaa ctcctccacc
     3721 gcgccccgat tctgatccgg tcgtgaagag cgctcgaccc cgtaggcgag ttcggcaacc
     3781 gtgatcgtcg agacggccat ctggtcggtg gcggctgcga accgcgcccg gagagcctcg
     3841 gctcgcgtgc ggagggcgaa gatgacgatg ttcgtgtcga ggaggtagcg cacgctcaca
     3901 gtgtgccgcg atcctccggc aggggctcgt ccctgtccac gaggaagtcc tcggtcaccg
     3961 tcggtccgtg agcgaaccag tgctcccacg ttctgcccct ggggctgacg aggcgacttg
     4021 agcccacgac cacgacgtcc acttctcgta cgctctcggg aaaggcgacc tccttcgcca
     4081 gccgcacgga ctgcgtgcga ttgttcttga agatcgtcgt gcgtgtcatg agggaagtat
     4141 ataccgggtg tatatacgac cacgatggca gcatgggaac atgcgacaca cgcccggcta
     4201 tctgctgacc gatcccgacg aggtcaagcg cctcatcagg gagaacccgt gggcgacgat
     4261 cgtctcccat acggccgccg gcctggtcgc atcccactac ccggtgatcc tcgaagagga
     4321 cgccgagggc atctcgatcg tcagccacgt cggccggccg gatgagaagc ttcacgagct
     4381 aggcgagcac gaggtgctcg tgatcatcca gggcccgcac ggctacatct cgcccggctg
     4441 gtacgacgaa ggggacttcg ttccgacctg gaaccacgtg accgctcacc tctacggcac
     4501 ccccgagctg ctcagcgacg aggagaactt cacagttctg gggcgactcg tcgacacatt
     4561 cgaagaccgg atgcccgagc ccgtgcacct cgacgtcgat gaggacgccg cccgccggat
     4621 cgcccgcggc acagtcggcc tgcggctgag agtcacgcgc ttcgacgctc gcctcaagct
     4681 gagccagaac aagagccccg aggtggtcga ccgcatcatc actgagcttc aggcagggga
     4741 gcagtactcg agccccggac tcgcccgcga gatgcgtcgg gtgcaccatg atccttcgta
     4801 acggtcgcct gggcggcgac accgtcgacc tgcgcctcgt cgatggtgtg atcgtggaga
     4861 tcggaacgat cgacgaaccc gggctcgacc tcggggggcg ctgggtcatc ccgggcctct
     4921 gggatcagca cgtgcacttc tcgcagtggg cgatgagtcg ccagcgcgtg gatgtctcgt
     4981 ccgcaacctc agcagccgag gcggcagccc tcgccgcagc cgctccgcgc ccgcccgccg
     5041 ggctggtact cgtgggcgtc ggataccgcg atggactctg gcccgatgag ccgagtctcg
     5101 aggtgctgga tgccgcgatc cccgacgtcc ccgtcgtgct catctccggc gacctgcact
     5161 cctgctggct caactcggcg gccgcgaccc gcttcggcgt caccgggccg ctgcttcgcg
     5221 aggaggactg cttcgccgtc gtggcagcgc tcgacgacgt gcccaccgag gtgctcgacg
     5281 ggtgggtggc tgacgccggc cgagcggctg cggcgcgcgg tgtcgtcggc gtcgtcgacc
     5341 tcgagatgac ctggaacatc gacaactggc tgcgccgcat ggccgcaggc ttcgacaccc
     5401 tacgggtgcg cagcggcatc tacccgcagt ttctcgaccg ggccatcgcc gccggctacc
     5461 gcacgggcac cgagctcggt ccgctgctgc acgtgggccc gttcaagatc atcaccgacg
     5521 gctcgctcaa cacccgcacc gccttctgcg tcgacccgta tcccggcatg ggcgactacc
     5581 gcgggctgct cacggtgcag ccccacgatc tggtcgagtg gctggctcgg gccgaggcgt
     5641 tcacgcccgc cgtgcacgcg atcggggacg cggccaacca cctcgccctc gatgcgttcg
     5701 aaagcctggg tgtcaccggc cgaatcgagc acgcgcagct cgtctccgac gacgacttcg
     5761 cgcgcttcgc cgaactcggc gtcatcgcca gcgttcaacc cgagcacacc atggatgacc
     5821 gcgatgtcgc agagcgctac tgggctgggc gcaccgggag gggctacgcg ctggcctcgc
     5881 tccacgatgc gggtgcgacg ctgctgctcg gctcggacgc tccg
//
LOCUS       DAOSOU010000125         5911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13620_length_5911_cov_7.531762, whole genome shotgun sequence.
ACCESSION   DAOSOU010000125 DAOSOU010000000
VERSION     DAOSOU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13620_length_5911_cov_7.531762"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLW15_07680"
     CDS             <1..525
                     /locus_tag="PLW15_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOT34308.1"
                     /translation="THDQTEALTMGDRIAVLKDGVLQQVGTPRDLYEAPQNVFVAGFI
                     GSPAMNLFDADVVEGGLKFGTAVAKVERATLGETKAKKVTIGIRPEDVKVAKTGEGLP
                     VDVDLVEELGADGYLYGHTEVDGNRVDIVARVDGRSHPNAGDKVFITPEPNHIHVFDT
                     ESGLRLSKKAVAAK"
     gene            637..1920
                     /locus_tag="PLW15_07685"
     CDS             637..1920
                     /locus_tag="PLW15_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOT34309.1"
                     /translation="MSGSLNITAATVDPALLDLPWNLPLEIWPEDSIAALPKGISRHI
                     VRFANLSGYVVAIKETSAELAKREYEMLRTLQRMDIPCVEPVAVITNRTSETGEILES
                     VLVTRHLRFSLPYRALYSSGLRPETATRLVDALAVLLVRLHIIGFFWGDVSLSNTLFR
                     RDAGAFAAYLVDAETGQLYDGLSNGQRENDLEIGRVNIAGELLDLQAGGRLDESIDPV
                     EISNGIVAKYRTLWKELTATEEFDQSERWRINSRVERLNDLGFDIEELAIKTDETGTQ
                     VRIQPKVVDAGHHSRRLLRLTGLDAQENQARRLLNDLDSYRAAFGKEDIDEEVVAHEW
                     TARVFEPVIRAIPRELRGKLEPAEIFHQLLDHRWYLSQNEGRDVPLAEAVSSYIDTVL
                     RHRRDEATMINPPTGTFTAPISLVDADDDDWRSKV"
     gene            1926..4172
                     /locus_tag="PLW15_07690"
     CDS             1926..4172
                     /locus_tag="PLW15_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT34310.1"
                     /translation="MGALESGEPGVLGRIAASLGRRPWLGLVVWAVLVGGAAAAALGG
                     ITGETLFERLVSGAPTATSESSRADDKLTSTASSAKSQTVTLLVHGVDLTDPAVTAIG
                     PDLVSLLGSDTVTIVDPLSIPTAPDGSRLPAVAPLFAEDGRGVLLTVKVSGVGDADPD
                     AVEVDRVVSVLKDTRDTLRSDLPDATVEVGSTQLLVDSIVAVSENDLQRGEGVALPIA
                     LLVMLVVFGGFIAAGVPLIGAIAAIIGALGALFGISHLMDIDTTVINVITAVGLGLSI
                     DYGLLMVSRFREEYRSALAGHPGADRRTLKLGAIARTADTAGRTVLYSGTTFAIASLG
                     LLVFEPRLVRAIGVGALSVTAIALASALTLVPALLGIAGDRLVRPGALTRLPLVGRAI
                     TRFGDVAPDEGVFSRLTRRVQRHPALITVLCALALLALASPVLSLRLANTSTDALPAS
                     SVQKVFVTTLSTEFPDAASPRIALVTDTEADATTWAAQVESLPRVTSVGTPTELGEGW
                     RTIVRVDPVDGIDVVREIRADRPDFPASVTGTDARTLDFSQSLLDRAPWAILIVALGT
                     ILLLFLMTGSLIVPLKALVASTLSLGASFGVLVWGFQWGNFAGLMVFDPTEVHGVDVL
                     VLVLTLAFGFGLAMDYEMFILSRIKELVDAGVEGREAIALGLQRSGRIISSAGLIIVV
                     VFAGFATGDLMVIKQLGVALAVAVVLDATLVRMLLVPAFMTWQERIMWWAPRWMKRIH
                     SRFGLREG"
     gene            complement(4190..4669)
                     /locus_tag="PLW15_07695"
     CDS             complement(4190..4669)
                     /locus_tag="PLW15_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT34311.1"
                     /translation="MAESMRSLDATDRALLRALSANARVTGSELAAMAGIAQSTVALR
                     LRRLQHDGYLKGSHADIDLAALGVTLQALIAVKLVKHVRTEVDAFRQAAPTWPGVLAL
                     FHMGGADDYLLHVASRSAEDLRQFVLTYLAGHPAVAHTETNLIFEHVVGHGLGDLLA"
     gene            4761..>5911
                     /locus_tag="PLW15_07700"
     CDS             4761..>5911
                     /locus_tag="PLW15_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOT34312.1"
                     /translation="MMRNDAHLETRAVHSGMAGLRESGGHVPPIDLSTTFPLASVESG
                     GDAYESLATGGDLLPGQTPVYQRLWQPGVARFEEALAALESAEAAVAFSTGMAALTAC
                     LLATVTAGKPHIVALRPLYGGTDHVLATGLLGTTVTWCDAASVSESIRDDTGFVILES
                     PANPTLELTDIRQVVADARTVPVLVDNTFATPVLQRPLELGATLVLHSGTKYLGGHGD
                     VMGGVVATSHEWTVRLRQVRALTGGLLHPLAAYLLQRGLRTLPVRVRAQQETAGELAR
                     RLVGHPAIARVLYPGLPGQDPAGLIGTQTAGAGALIALDLADGYAAASRFAESCELIT
                     HAVSLGGIDSLVEHPASLTHRPVAADAKPGAGVVRLSIGLEHVDDLYAD"
BASE COUNT          915 a         2026 c         1964 g         1006 t
ORIGIN      
        1 acgcacgacc agaccgaggc gctcaccatg ggtgaccgca tcgccgtgct caaggatggc
       61 gtgctccagc aggtgggtac cccccgcgac ctctacgagg ctccgcagaa cgtcttcgtc
      121 gcaggcttca tcggaagccc cgcgatgaac ctgttcgacg ctgacgtcgt cgagggcggc
      181 ctcaagttcg gcaccgcggt cgccaaggtc gagcgcgcca cgctcggtga gacgaaggcc
      241 aagaaggtca cgatcggaat ccgtcccgaa gacgtcaagg tcgccaagac cggcgagggc
      301 ctccccgtcg acgtcgacct ggtcgaggag ctcggcgccg acggctacct ctacggacac
      361 accgaggtcg acggcaaccg cgtcgacatc gtcgcccgcg tcgatggtcg ttcgcacccg
      421 aacgccggtg acaaggtctt catcaccccg gagccgaacc acatccacgt gttcgacacc
      481 gagtccggcc tgcgcctgag caagaaggca gtcgccgcga agtagttcag atccaccgcc
      541 ggtcgagtag ccgcgagagc ggcgtatcga gaccctccca cgggatctcg atacgcgctc
      601 cgcgctactc gatcggcagt taaaggggca caccacatga gcggttcact caacatcacg
      661 gcggccacgg tcgaccccgc cctgctcgac cttccgtgga acctgcctct ggagatctgg
      721 cccgaggatt cgatcgcggc actgcccaag ggcatctcgc gccacatcgt gcggttcgcg
      781 aacctcagcg gctacgtcgt ggcgatcaag gagacatccg ccgaactggc caagcgtgag
      841 tacgagatgc tgcgcacgct gcagcgcatg gacatcccgt gcgtcgaacc ggtcgcggtg
      901 atcaccaacc gcacgtcgga gacgggcgag attctcgagt cggtgctcgt cacccgtcac
      961 ctacggttct cgcttcccta ccgcgcgctc tactcgtcgg gcctccgtcc cgagaccgcg
     1021 acccgtctgg tcgatgccct cgccgtgctg ctcgtgcgtc tgcacatcat cgggttcttc
     1081 tggggtgacg tgtcgctgtc gaacaccctc ttccgacggg atgccggcgc cttcgccgcc
     1141 tacctcgtcg atgccgagac cgggcagctg tacgacggcc tctccaacgg tcagcgcgag
     1201 aacgacctcg agatcggtcg tgtcaacatc gcgggcgagt tgctcgacct gcaggccggt
     1261 ggtcgcctcg acgaatccat cgaccccgtc gagatcagca acggcattgt cgcgaagtac
     1321 cgcacgctgt ggaaggaact gacggcgacg gaggagttcg accagtccga gcggtggcgc
     1381 atcaacagtc gcgtcgagcg tctcaatgac ctcggcttcg acatcgagga gttggcgatc
     1441 aagacggatg agacgggcac ccaggtgcgc atccaaccga aggtcgtcga cgccggccac
     1501 cactcccgcc gcctgctgcg tctgaccgga ctcgacgccc aggagaacca ggcccgccgc
     1561 ctcctcaacg atctggactc ctaccgggcc gccttcggca aggaggacat cgatgaggag
     1621 gtcgtcgctc acgagtggac ggccagggtc ttcgagccgg tcatcagggc gatcccgcgg
     1681 gagcttcgcg gcaagctgga gcccgcggag atcttccacc aactgctcga tcaccgctgg
     1741 tacctctcgc agaacgaggg acgagacgtc cccctcgccg aggcggtctc gagctacatc
     1801 gacacggtgc tgaggcaccg gcgcgacgag gcgacgatga tcaaccctcc gacgggcacc
     1861 ttcacagcgc cgatctccct ggtggacgct gacgacgacg actggcgcag caaggtctag
     1921 gcgcggtggg ggcgctggag tcgggcgagc ccggcgtgct ggggaggatc gcggcatccc
     1981 tcggtcgtcg accgtggctg gggctggtgg tgtgggccgt gctcgtcggc ggtgctgcgg
     2041 cagccgcgct gggcggcatc accggtgaga ccctcttcga gcgactcgtg agtggtgcac
     2101 ccacggcgac gagcgagagc tcgcgggccg atgacaaact gacgagcacg gcctccagcg
     2161 cgaagtccca gacggtgact ctgctggtgc acggggtcga cctgacggat cccgccgtca
     2221 cagcgatcgg gcccgatctc gtgagcttgc tcggcagtga cacggtcacc atcgttgatc
     2281 cgctgtcgat ccccaccgct cccgacggga gtcgcctgcc ggcggtggct ccgctcttcg
     2341 ccgaggatgg ccgtggggtg ctgctcaccg tgaaggtatc cggggttggc gacgccgacc
     2401 cggatgctgt cgaggtcgac cgcgtcgtct cggtgctgaa ggacactcga gacaccctgc
     2461 gctccgacct ccccgacgcg acagtcgagg tgggctccac tcagctgctg gtcgactcga
     2521 tcgtcgccgt gtcggagaac gacctccagc gcggtgaggg cgtcgcgctg ccgatcgccc
     2581 tgctcgtgat gctcgtcgtc ttcggcgggt tcatcgccgc cggtgtgccg ttgatcgggg
     2641 ccatagcggc gatcatcggc gcgctcggtg ccctgttcgg catcagccat ctcatggaca
     2701 tcgacacgac ggtcatcaac gtgatcacgg cggtcgggct cggcctgtcg atcgactacg
     2761 gcctgctgat ggtgagccgc ttccgcgagg agtaccgctc tgccctggct gggcatccgg
     2821 gggcagaccg gcgcacgctc aagctcggcg ctatcgcccg caccgctgac accgctgggc
     2881 gcaccgtgct ctactccggc accaccttcg ccatcgcgag cctcgggctg ctcgtgttcg
     2941 agccgcgcct ggtgcgcgcg atcggggtgg gtgcgctcag cgtcaccgcc atcgcgctgg
     3001 cgtcggcgct cacgctcgtc cccgcgctgc tcggaatcgc cggcgatcgg ctggtgcgtc
     3061 cgggggcgct cacgcggctt ccgctggtgg gcagggcaat cacccggttc ggggatgtcg
     3121 cgcccgatga aggggtgttc tctcggctca cacgtcgagt gcagaggcat ccggccctca
     3181 tcacagtgct gtgcgctctc gcgctgctgg cgctcgcgag cccggtgctg agcctgcggc
     3241 tggcgaacac gagcaccgac gcgctgcccg cctcgtcggt gcagaaggtc ttcgtgacga
     3301 cgctgagcac cgagttcccg gatgccgcga gcccccgcat cgcgctcgtc accgacaccg
     3361 aggccgacgc caccacctgg gccgcccagg tcgagtccct gccgcgggtc accagcgtcg
     3421 gaaccccgac cgagctcggc gagggttggc gcacgatcgt gcgcgtcgac ccggtcgacg
     3481 gcatcgacgt ggtgcgggag atccgcgccg atcgacccga cttccccgcg tcggtgaccg
     3541 gcacggatgc ccgcaccctc gacttctcgc agtcgctact cgaccgggcg ccgtgggcga
     3601 tcctcatcgt cgcgctcggc accatcctgc tgctgttcct catgacgggg tcgctgatcg
     3661 tgccgctgaa ggcactggtc gcgagcaccc tgtcgctcgg tgcgtcgttc ggcgtgctcg
     3721 tctggggatt ccagtggggc aacttcgccg ggctcatggt gttcgacccg acggaggtgc
     3781 acggcgtcga cgtgctcgta ctcgtgctca ccctcgcctt cgggttcggg ctcgcgatgg
     3841 actacgagat gttcatcctg tcgcgcatca aggagctcgt cgatgccggc gtcgagggcc
     3901 gggaagcgat cgcgctcggc ctgcagcgct ccggtcgcat catcagctcg gcggggctca
     3961 tcatcgtcgt cgtgttcgcg ggattcgcca cgggcgatct catggtgatc aagcagctgg
     4021 gtgtcgcgct cgcggtcgcg gtcgtgctgg atgccacgct ggtgcggatg ctgctcgtgc
     4081 ccgcgttcat gacctggcag gagcgcatca tgtggtgggc gccgcgctgg atgaagcgca
     4141 tccactcccg gttcgggttg cgcgagggct gagcctgtcg ccccgcacgt caggcgagca
     4201 agtcgccgag cccgtggccg acgacgtgtt cgaagatcag gttcgtctcg gtgtgcgcca
     4261 ccgccggatg acccgccaga taggtcagaa cgaattggcg caggtcttcc gcgctcctcg
     4321 aggcgacgtg tagcaggtag tcgtccgccc cgcccatgtg aaacagcgca aggactccgg
     4381 gccaggtcgg cgccgcctga cgaaacgcat ccacctcggt tcgaacgtgc ttgaccagct
     4441 tgaccgcgat cagcgcttgg agggtaaccc cgagcgcggc caggtcgatg tcggcgtgcg
     4501 aacccttcag gtagccatcg tgctgaaggc ggcgcaggcg cagtgcgacg gtggactgag
     4561 cgatgcccgc catcgcggcc agttcagaac cggttacgcg cgcgttcgcc gagagcgccc
     4621 gcaggagggc ccggtctgtt gcatccagag acctcatcga ttcggccact atctcgcact
     4681 cccttcatca atgtggtgca tctgtcgcag aatctgcaga gttcgttgac tagtgtcgca
     4741 ctttcggctc gactcgaccc atgatgcgca acgatgccca cctcgaaacc cgtgctgtgc
     4801 actccggaat ggcaggcctc cgagagagcg gaggccatgt tccgcccatc gatctctcga
     4861 cgaccttccc gctcgcgagc gtcgagtccg gcggggatgc ctacgagtcg ctcgcgaccg
     4921 ggggcgatct gctgcccggc cagactccgg tgtatcagcg actgtggcag cccggggtgg
     4981 ctcgattcga ggaggcgctg gccgcgctcg agagcgccga ggccgcggtg gcgttctcga
     5041 cggggatggc cgctctcacg gcctgtcttc tcgcgaccgt gactgcggga aagccgcaca
     5101 tcgttgcgct gcgtccgctg tacggcggta cggatcacgt actggcgacc gggttgctcg
     5161 gcaccaccgt cacgtggtgc gacgcggcct ccgtctccga gtcgatccgg gatgacacgg
     5221 gcttcgtgat tctcgagagc ccggcgaatc ccacactcga actcaccgac atccgacaag
     5281 tggtagcgga tgcccgtacc gtgcccgttc tcgtcgacaa cacattcgcg acgccggtcc
     5341 tgcagcgtcc tctcgaactg ggtgcgaccc tcgtgttgca cagcggcacg aagtacctcg
     5401 gcggacacgg tgacgtcatg ggcggcgtcg ttgccacgag ccacgagtgg acggtacgcc
     5461 ttcgtcaggt gcgcgcgctc acggggggtc tgctgcatcc gctcgccgca tacctgcttc
     5521 agcgagggct gcgcacgttg cccgtgcggg tacgcgcgca acaggagacg gccggcgaac
     5581 tggctcggcg tctcgtcggc catccggcaa ttgcccgggt gctctacccg ggattgcccg
     5641 ggcaggaccc cgcaggactc atcggaacgc aaactgccgg ggcgggtgcg ctcatcgcgc
     5701 tcgacctcgc cgacggctat gccgccgcat cccgcttcgc cgaatcgtgc gagctcatca
     5761 cccacgccgt gtcgttggga ggcatcgact cgctcgtcga acatccggct tcgctgacac
     5821 atcgccccgt cgcggcagac gcaaagccgg gggccggcgt tgtgcggctg tcgatcggtc
     5881 tggagcacgt cgacgatctc tacgcggata t
//
LOCUS       DAOSOU010000126         5890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13685_length_5890_cov_5.389546, whole genome shotgun sequence.
ACCESSION   DAOSOU010000126 DAOSOU010000000
VERSION     DAOSOU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13685_length_5890_cov_5.389546"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1875)
                     /gene="hrpA"
                     /locus_tag="PLW15_07705"
     CDS             complement(<1..>1875)
                     /gene="hrpA"
                     /locus_tag="PLW15_07705"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOT34313.1"
                     /translation="LARMVIESKQHGTTREVIAIVAGLSIQDPRERPLERRAQADQQH
                     ARFVDPTSDFLTLLNLWNYLEEKQKELSGNAFRRLVKSEYLNYLRVREWQDVFRQLSR
                     AAKPLGLHVGEAHVNPDGIHRSILAGLLSQIGLRDLAKKDYVGARQSRFVIFPGSALA
                     KKQPAAIMSAELVETSRLFARTNGAIDPAWAEKIAGDLAKRSYSEPHWEKKQGAVVAY
                     ERVTLYGVPIIAKRRVQFSRIDPAYARELFIRHALVDGEWDLDRVDQRLAAFDRANRA
                     LRAQLAELEERTRRRDILYDDEAIFEFYLRRIPADVASTRDFEGWWKKARLETPDLLT
                     MTPENLMPDDAPVVDEEAFPSSWTHGDQTLALTYRFEPGAEDDGVTVAVPLPLLAGLR
                     SDGFDWQVPGLREELVTALIKSLPKHIRKNVVPAADWARKLLVPVVERSRDLPHGSRL
                     RSITEELAAEITAQTYTPVTAADFDLDRVPEHLKVTFAVFDEKGKRVAAGKTLSALQA
                     SLKPAARESVARVVETPRDSLERAGLTTWDFDALDRVRDTRHGGNTIRAYPALVDEGS
                     SVSIRLFGTPHDQAVAHRAGVRRLLLKAIPSPTGYVQEHLTTQEKLVLAQSPYRTTAE
                     "
     assembly_gap    1876..1975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1976..3141)
                     /locus_tag="PLW15_07710"
     CDS             complement(<1976..3141)
                     /locus_tag="PLW15_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT34314.1"
                     /translation="MSEDLPPITYPPELPVSQRRDDIADALRANQVIIVAGATGSGKT
                     TQLPKILLELGKRSIGHTQPRRIAARTIAERVAQELGQEVGGLVGYQVRFTDRVGPQT
                     RIKLMTDGILLNEIHRDRDLKKYDAIIIDEAHERSLTVDFLLGYLKQLLPRRPDLTVV
                     ITSATIDPESFARHFADAAGTPAPIIEVSGRTYPVEIRYRPLVAEALEAEDADLDKLD
                     QQTTPVDRDYLQGITDALDELGREGDGDVLVFLSGENEIRDAEEAIRGRISAGSMTAG
                     TEVLPLYGRLSAADQHRVFETSRTPGVRRRVVLATNVAETSLTVPGIRYVIDAGTARI
                     SRYSVRSKIQRLPIEAISQASANQRSGRSGRTSDGIAIRLYSEEDFARRPEFTEP"
     gene            3178..3672
                     /locus_tag="PLW15_07715"
     CDS             3178..3672
                     /locus_tag="PLW15_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT34315.1"
                     /translation="MTSGNRFTDEELAAWRPNLISWQLIERGLERQLQRDSGMPHAYY
                     AILVVLGEQHSMPLAALAAELDYSPSRMSHAVARLEESGWVERVPDASDRRVTLLRLT
                     GEGRRALAAASVGHVGQVRGSFFVHLDDRDLADLARIGRKLYDGLTGGRPGPLSGPQV
                     AQRP"
     gene            3682..4512
                     /locus_tag="PLW15_07720"
     CDS             3682..4512
                     /locus_tag="PLW15_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT34316.1"
                     /translation="MTTPRITLNDGTTIPQLGFGVFRVDPEETERIVSDALEVGYRHI
                     DTAAIYGNEIGVGRAIAASGIPRDELYITTKLWNSDQGTQSAIDAMDLSLEKLGLDHV
                     DLYLIHWPRPDLDRYLESWHTLEQLKADGKTTSIGVSNFHIPHLERVIGESGTVPAVD
                     QIELHPAFAQRPIREYAASKGIAIEAWGPLGQGKYDLFGMPAVADAARAHGVSPAQAV
                     IRWHLQHGIIVFPKSNSRERIAQNFDVFGFELTAAEMAAIDGLDQGMRVGSNPDEATF
                     "
     gene            complement(4593..5348)
                     /locus_tag="PLW15_07725"
     CDS             complement(4593..5348)
                     /locus_tag="PLW15_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT34317.1"
                     /translation="MSRTTITYIGGPTALLEYAGLRFVTDPTFDTPQTYAEPGSTTLV
                     KTTGPGIPRSALGHVDAVLLSHHSHRDNLDYEGLELIATGVPTLSTRDAASDLFGGSV
                     IGFDSWEIHRLGDVTITAVPALHGPPGSEPWVGKVTGFVLEAPGEPTVYVSGDNASLA
                     LVEQIANRFPEIDIAVLFAGAATVPRIPSRLTLSAADARQAAELLGASRVVGLHTEDW
                     EHFAEDAAQFAAAFEGSGLLVPTPRGETVTLAG"
     gene            complement(5365..>5890)
                     /locus_tag="PLW15_07730"
     CDS             complement(5365..>5890)
                     /locus_tag="PLW15_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT34318.1"
                     /translation="RRLHELLWYLDCAIAFTTDDAIGAALAQQFESVRRLTAGEPAAV
                     RDIDTDVLYDEARPLLVRASAAHRGTGTGSRPDRRIRPGADLVGADLRRADLRAADLR
                     GALLIAADLRGVDLSRCDLLGVDLRDANVGGADLTAALFLTQPQVSAAIGDAATLLPE
                     GFDRPAHWERNPLI"
BASE COUNT          878 a         1928 c         2032 g          952 t
ORIGIN      
        1 gttcggccgt cgtgcggtac gggctctggg cgagcaccag cttctcctgg gtggtcaggt
       61 gctcctgcac gtacccggtc ggggagggga tggccttcag cagcagccga cgcaccccgg
      121 cgcgatgggc gacggcttga tcgtgcgggg tgccgaacag ccggatcgac acgctcgatc
      181 cctcgtcgac gagtgcgggg taggcccgga tcgtgttgcc gccgtgccgg gtatcccgca
      241 cccggtcgag ggcgtcgaag tcccaggtgg tcagtccggc acgttcgagg ctgtcgcgcg
      301 gggtctcgac cacgcgcgcg accgattcgc gtgccgcggg cttgaggctc gcctgcaggg
      361 cgctgagcgt cttgcctgcg gcgacgcgct tgcccttctc gtcgaagacc gcgaaggtga
      421 ccttcaggtg ctcgggcaca cggtcgagat cgaagtcggc ggcggtcacg ggcgtgtagg
      481 tctgtgcggt gatctcggct gcgagctcct cggtgatcga gcgcagtcga gatccgtgcg
      541 gcaggtcccg gcttcgctcg accaccggca cgagcagctt gcgcgcccag tcggcggcgg
      601 gaacgacgtt cttgcggatg tgcttgggca ggctcttgat gagcgcggtc acgagctcct
      661 cgcgcaggcc gggcacctgc cagtcgaagc cgtccgatcg caggccggcg agcaggggca
      721 ggggcaccgc gacggtgacg ccatcgtctt cggcgccggg ttcgaaccgg taggtcagag
      781 cgagagtctg gtcgccgtgc gtccagctgc tcgggaacgc ctcctcgtcg acgaccggag
      841 cgtcgtcggg catgaggttc tcgggcgtca tggtgagcag gtcgggtgtc tcgaggcgcg
      901 ccttcttcca ccagccctcg aagtcgcggg tcgacgcgac atccgccggg atgcgacgca
      961 ggtagaactc gaagatcgcc tcgtcgtcgt acaggatgtc gcggcgccgg gtccgctctt
     1021 cgagctcggc gagctgcgca cgcagggccc ggttggcccg atcgaacgcg gccagccgct
     1081 gatcgacccg gtcgaggtcc cactcaccat ccacgagcgc gtgccggatg aacagctcgc
     1141 gcgcgtaggc agggtcgatg cgggagaact gcacccggcg cttcgcgatg atgggcacgc
     1201 cgtagagggt cacccgctcg tacgcgacga cggccccctg cttcttctcc cagtgcggct
     1261 ccgagtagct gcgcttggcg agatcgccgg cgatcttctc cgcccacgcc ggatcgatcg
     1321 ctccgttggt gcgcgcaaac aggcggctgg tctccacgag ctcggcgctc atgatcgcgg
     1381 cgggctgctt cttcgcgagg gccgatcccg ggaagatcac gaagcgactc tggcgtgccc
     1441 ccacgtagtc cttcttcgcg agatcgcgca ggccgatctg gctcagcagc cccgcgagga
     1501 tcgatcgatg gatgccgtca gggttgacgt gcgcctcgcc cacgtgcagc ccgaggggtt
     1561 tggcagctcg gctcaactgg cggaacacgt cctgccactc ccgcacccgc aggtagttga
     1621 ggtactccga cttcacgagt cgacggaatg cgttgcccga cagctccttc tgcttctcct
     1681 cgaggtagtt ccacaggttc agcagcgtga ggaagtcgct cgtcggatcg acgaagcgcg
     1741 cgtgctgctg atcggcctgg gctcgccgct cgagcggacg ttcccggggg tcctggatgc
     1801 tgagacccgc aacgatcgcg atcacctcgc gggtggtgcc gtgctgcttc gactcgatca
     1861 ccatgcgggc gagccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggctc
     1981 ggtgaactcg ggccgccggg cgaaatcctc ctcgctgtac agccggatcg cgatgccgtc
     2041 gctcgtgcga ccagagcgcc ccgagcgctg gttcgcgctg gcttgactga tcgcctcgat
     2101 cggcagccgc tgaatcttcg agcgcacgct gtaccggctg atgcgggcgg tgcccgcgtc
     2161 gatgacatag cggatgcccg gcaccgtcaa cgacgtctcc gccacgttcg tcgccagcac
     2221 aacccggcgc cgaacgcccg gagtgcgcga cgtctcgaac acccgatgct ggtcggcggc
     2281 gctgagtcga ccgtagaggg gcagcacctc ggtgcccgcg gtcatcgagc ccgccgagat
     2341 gcgtccgcgg atggcctcct cggcatcccg gatctcgttc tcacccgaga ggaacaccag
     2401 cacgtcgccg tcgccctcgc ggccgagctc gtcgagggcg tcggtgatgc cctgcaggta
     2461 gtcccggtcg acgggggtcg tctgctgatc gagcttgtcg agatccgcgt cctcggcctc
     2521 gagagcctcc gccaccagcg gtcggtaacg gatctcgacg gggtaggtgc gcccggatac
     2581 ctcgatgatc ggggcgggtg tgccggcggc atccgcgaag tgccgggcga agctctcggg
     2641 gtcgatggtc gcactcgtga tcacgacagt gagatcgggt cggcggggga gcagttgctt
     2701 gaggtagccg agcaggaagt cgacggtgag actgcgctcg tgggcctcgt cgatgatgat
     2761 cgcgtcgtac ttcttcaggt cgcggtcgcg gtggatctcg ttgagcagga ttccgtcagt
     2821 catgagcttg atgcgggtct gcggccccac ccgatcggtg aaacgcacct ggtatccgac
     2881 caggccaccg acctcctggc cgagttcctg cgcgacgcgc tcggcgatcg tgcgggccgc
     2941 gatccgccgc ggctgggtgt gcccgatgct gcgcttgccg agctcgagaa ggatcttggg
     3001 cagctgggtc gtcttgcccg agcccgtggc gccggcgacg atgatcacct ggttcgcgcg
     3061 cagggcgtcg gcaatgtcat cccgccgctg gctgaccggc agctcggggg gataggtgat
     3121 cggggggagg tcctcggaca tgagccttac agtttatggc cccgtagggt ggaggccatg
     3181 acgtccggca atcgcttcac cgacgaggag ttggcggcct ggcgccccaa cctgatcagc
     3241 tggcagttga tcgagcgcgg cctcgagcgg cagctccaga gagactccgg gatgccgcac
     3301 gcgtactacg ccatcctcgt cgtgctgggt gagcagcact cgatgccgct cgccgctctc
     3361 gccgcagaac tcgactactc gccgagccgc atgtcccacg cggtcgcgcg gctcgaggag
     3421 tcggggtggg tcgagcgagt gccggacgcg agcgatcgga gggtgacgtt gctgcgcctc
     3481 acgggggagg ggcggagggc tcttgccgcg gcatccgtgg ggcatgtcgg tcaggtgcgc
     3541 ggcagcttct tcgtccacct cgacgaccgt gacctcgccg atctggcacg gatcgggcgc
     3601 aagctctacg acggtctcac gggcggtcgg cccggacccc tgagcggtcc gcaggtcgcg
     3661 cagcggccgt aggctggagg gatgacgaca ccccgcatca cgctcaacga cggcaccacg
     3721 attccccagc tggggttcgg cgtcttccgg gtcgacccgg aggagacgga acggatcgtc
     3781 tccgacgcgc tcgaggtcgg gtaccgtcac atcgacaccg ccgcgatcta cggcaacgag
     3841 atcggggtcg gtcgtgcgat cgcggcgtca ggaattcccc gcgatgagtt gtacatcacg
     3901 accaagctgt ggaacagcga ccagggcacc cagtcggcga tcgacgccat ggacctcagc
     3961 ctcgagaagc tcgggctcga ccacgtcgac ctctacctga ttcactggcc ccgccccgat
     4021 ctggaccgct acctcgagag ctggcacacc ctcgagcagc tgaaggccga cggcaagacg
     4081 acctcgatcg gggtctccaa cttccacatc ccacacctcg agcgcgtgat cggcgagagc
     4141 ggcaccgtgc cggccgtcga tcagatcgag ctgcaccccg cattcgctca gcgaccgatc
     4201 cgcgaatacg cggcatcgaa gggcatcgcg atcgaggcgt gggggccgct cgggcagggc
     4261 aagtacgacc tgttcgggat gcccgctgtc gccgacgccg cgcgggctca cggtgtgtcg
     4321 cccgcgcagg ccgtcattcg gtggcacctg cagcacggca tcatcgtctt tcccaagtcg
     4381 aattcccgtg agcgcatcgc ccagaacttc gacgtcttcg gcttcgagct gacggccgcg
     4441 gagatggcgg ccatcgacgg actcgaccag gggatgcggg tcggttcgaa tcccgacgag
     4501 gcgactttct gacgtagagg ccgattgagt agcgccgaag gcgcgtatcg aaatccgcaa
     4561 ccgagccggt ttcgatacgg gctccgccct actcaaccag ccagcgtcac cgtctcaccg
     4621 cggggcgtgg gcaccagtag cccgctgccc tcgaacgccg ctgcgaactg cgcggcgtct
     4681 tcggcgaagt gctcccagtc ctcggtgtga agaccgacga cacgggatgc gccgagcagc
     4741 tcggccgcct ggcgggcgtc ggccgcggag agggtgagcc tcgacgggat gcgcggcacc
     4801 gttgccgcgc ccgcgaagag aaccgcgatg tcgatctccg ggaagcgatt cgcgatctgt
     4861 tcgacaagcg caagcgacgc gttgtcgccg ctcacataga cagtgggttc tcccggtgcc
     4921 tccagcacga atcccgtgac cttgcccacc cacggctcgc tcccgggcgg gccgtgcagc
     4981 gccggcaccg cggtgatcgt gacatcgccc aggcggtgaa tctcccacga gtcgaagccg
     5041 atcaccgacc cgccgaagag gtcggacgcg gcgtcccttg tgctcagggt cgggacgccc
     5101 gtcgcgatca actccagacc ctcgtagtcg aggttgtcgc gatgtgagtg gtggctgagc
     5161 agcacggcgt cgacgtggcc gagcgccgac ctcgggatgc ccggcccggt cgtcttgacg
     5221 agtgtggtcg atcccggctc ggcgtacgtc tgcggcgtgt cgaatgtcgg gtcagtgacg
     5281 aagcgcagcc ccgcgtactc gagcagggcg gtcggcccgc cgatgtaggt gatggtcgtc
     5341 cgcgacatac ctcgacacta gccgtcagat cagcgggttg cgctcccaat gcgcgggccg
     5401 gtcgaagccc tccggcagta gcgtcgcggc gtctccgatg gccgcggaga cctggggttg
     5461 ggtgaggaac agtgccgccg tcaggtcggc cccgccgacg ttcgcgtccc tcaggtcgac
     5521 acccagcagg tcgcaccggc tgaggtcgac gcccctgaga tcggctgcga tcaggagcgc
     5581 cccacgaaga tcggccgctc tcagatccgc ccggcgaagg tcggcgccaa ccaggtcggc
     5641 accgggccgg attcgtcggt cggggcgtga ccccgtaccg gtgccccgat gtgcggcgct
     5701 ggctcgcacg agcagcgggc gcgcctcgtc gtagagcacg tcggtgtcga tgtcgcgcac
     5761 agcagcgggc tcgcccgccg tgagtcgacg aaccgactcg aactgctgtg cgagggccgc
     5821 gccgatcgcg tcgtcggtgg tgaatgcgat ggcgcaatcc aggtaccaga ggagctcatg
     5881 aagccgacga
//
LOCUS       DAOSOU010000127         5873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13736_length_5873_cov_7.138879, whole genome shotgun sequence.
ACCESSION   DAOSOU010000127 DAOSOU010000000
VERSION     DAOSOU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13736_length_5873_cov_7.138879"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLW15_07735"
     CDS             <1..454
                     /locus_tag="PLW15_07735"
                     /inference="COORDINATES: protein motif:HMM:NF040070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HOT34319.1"
                     /translation="GFAIGTGSNVSALGTQLGPIPAIPLLGALPTGSYAFGFVGLLVP
                     VIAGFLAGALVGPGVRRRLGAEAGGSAVLLTGLASGVVGGVILGMLAWASSGAVGPGR
                     LAHVGPDPWAVGGFAALEFALATTIGLAASLRRARATQPTPGATTTRR"
     gene            464..1048
                     /gene="purN"
                     /locus_tag="PLW15_07740"
     CDS             464..1048
                     /gene="purN"
                     /locus_tag="PLW15_07740"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOT34320.1"
                     /translation="MLSLVVLISGGGSNLRALLEASEDAEFPARVVAVGADRPADGFE
                     HAEHFGVPTFSVALSAFDDRDTWGDALLEQVRVWQPDLVVLSGFMKLLPPRVVAALAP
                     NLINTHPAYLPEFPGAHAVRDAIAAGVTETGASVIVVDNGVDSGPIIAQERIPVLPGD
                     TETELHERIKPVERRLLVQAILDIANGHIDLKEL"
     gene            1049..2680
                     /gene="purH"
                     /locus_tag="PLW15_07745"
     CDS             1049..2680
                     /gene="purH"
                     /locus_tag="PLW15_07745"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT34321.1"
                     /translation="MSGPSHDPSDYRERDAIPIRRALVSVSDKTGLVELATALAAAGV
                     EIVSTGSTAKTIRDAAIPVTEVAEVTGFPESLDGRVRTLHPAIHAGLLADLRLASHES
                     ELAELGIAPFELVVSNLYPFVETVASGAAVNDVIEQIDIGGPALVRASAKNHANVAIV
                     TSPASYPQVLKALTLGGTTLAQRQRLAGEAFAHTAAYDTAVAAYFAANVGIKNPVVET
                     PVAEALEATFPQTLTYEVELSTGLRYGENSHQQAALYVEPAGRGIAQATQLQGKEMSY
                     NNYVDADAALRAAYDFDEPAVAIIKHANPCGIAVARGTGDPIASAHARAHACDPVSAY
                     GGVIAANRTLTLEAAESIKDIFTEVIVAPAFEPAALELLASKKNLRLLQLPAGYGRSD
                     TEFRQISGGALVQTPDTYVDFSSDAWTLVAGPEADDATRADLEFAWRAVRAVKSNAIL
                     LAKDGASVGVGMGQVNRVDSCGLAVSRAADRAIGSVAASDAFFPFADGLEILLEAGVR
                     AIVQPGGSIRDEEVIAAAQDAGATMYFTGERHFFH"
     gene            2830..4632
                     /locus_tag="PLW15_07750"
     CDS             2830..4632
                     /locus_tag="PLW15_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT34322.1"
                     /translation="MSAPTAEPGRADTVGTFAAIRRLYPYAKPAMPRIYLGMLSALLA
                     GLVALLIPQVLKSLVDGPLQSGDSAQIWPAFLVVLGLGILEAIMIALRRFWVLTPGTH
                     IEARMRNALYMKLQTLPVSFHDRWPSGQLLSRAVSDLNLIRRWISFGLVLLVVNVLTI
                     LVGFAFLVNISWILGVLFIVCSIPLWVYGYLFEKRYSIVARRSQDQAGDLATTVEESV
                     HGIRVLKAFGRGKHALENFATQAEELRGTEIEKAKAIAGIWLWLLLVPDVTFALALLG
                     GVWLTATGQISAGDLVAFFATATVLRFPVESIGFLLSMTFDTRTAADRFFEVMDAPNA
                     IVDPEHPKTIEHPEGRLVFTDVHFRYPDSPERVPDLVDGVSLAIEPGETMALVGLTGS
                     GKSTLTALTTRLYDIDSGSIAIDGVDIRDLTLGELRTHIAMAFEDATLFSATVRDNVL
                     LGRPDATEADLAEALDIAQAAFVHELPDGLDTAVGEEGLSLSGGQRQRLALARAVAAK
                     PSILVLDDPLSALDVDTEALVEAALRRVLASTTALIVAHRPSTVMLADRVALLEHGRV
                     TAVGTHSELLASSEHYRFVISSLEDEQRRERAQA"
     gene            4629..>5873
                     /locus_tag="PLW15_07755"
     CDS             4629..>5873
                     /locus_tag="PLW15_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOT34323.1"
                     /translation="MSAVSGVGGEERTDYSKTESRQIRQRSLRLLGSLVLPVRARVIL
                     TMVVVVVSTAAQVAGPLFIAYGIDTGLPALLNQDWMPLAGAVTAYLAAGILGAALIAW
                     YTVLTARISQAILFDLRKRVFLHTQRLSLEFHESYTSGRIIARQTSDLDAIRELLDSG
                     INQLVQGLLYMVFIAIAMFTLDGLSGLILVASLVPLLLLTRWFQVRSQRFFRTTRTAS
                     ATLIVKFVETMTGIRAVKAFRTEESNRTVFGGLVENYRRTNQRVIQLFGVFDPGLVLI
                     GNVTLAVILLVGGLRVATGGIAIGVLLAALLYARQFFAPAQEMAMFYNSYQSAAAALE
                     KISGVLEEQPSVPDPTKPVDLLKAQGRVTFDAVTFSYKDDRVVLPNLELEIPAGQTIA
                     LVGTTGAGKSTLAKLISRFYDPT"
BASE COUNT          858 a         2133 c         1936 g          946 t
ORIGIN      
        1 cggtttcgcg atcggcaccg ggtccaatgt cagcgcgctc ggaacgcagc tcggacccat
       61 cccggcgatc ccgcttctcg gcgccctgcc gaccggcagc tacgccttcg gattcgtggg
      121 gctcctcgtt cccgtgatcg cgggcttcct cgcgggggcg ctggtcggtc ccggcgtgcg
      181 ccgacgcctg ggcgccgagg ccggcggcag cgcggtactg ctcaccggac tggcctcggg
      241 ggtcgtcggt ggtgtcatcc tcgggatgct cgcctgggcg tcatccggag cggtcggccc
      301 cgggcggctc gcccacgtcg ggcccgaccc ctgggcggtg ggcggattcg cggcgctcga
      361 gttcgcgctc gcgacgacca tcggtctcgc ggcgagcctg cgccgcgccc gtgcgaccca
      421 gcccacgccg ggcgcgacca cgacccgacg ataggctgtc ggggtgctgt cactcgtggt
      481 gctgatctcg gggggcgggt ccaacctccg ggccctgctc gaagcctcgg aggacgccga
      541 gttcccggcc cgcgtcgtcg ccgtcggagc cgatcgaccg gcggacgggt tcgagcacgc
      601 cgagcatttc ggggtcccca ccttctccgt cgccctgtcg gcgttcgacg atcgcgacac
      661 ctggggcgat gcgctacttg agcaggtgcg ggtctggcag cccgacctcg tggtgctcag
      721 cggattcatg aagctactgc cgccgcgggt ggtcgcggcg ctcgcgccca acctgatcaa
      781 cacgcatccg gcctacctgc ccgaattccc gggcgctcac gcggtgcgtg acgcgatcgc
      841 cgccggggtg accgagaccg gcgcgagtgt gatcgtcgtc gataacggcg ttgacagcgg
      901 gccgatcatc gcccaggagc gcatccccgt gctgcccggc gacaccgaga ctgaattgca
      961 cgagcgcatc aagcccgtcg aacgccgcct gctcgtgcag gcgatcctcg acatcgccaa
     1021 tggccacatc gacctgaagg agctctaagt gtcaggcccg agtcacgacc cgtccgacta
     1081 ccgcgagcgc gatgcgattc cgatccgtcg cgccctcgtc tccgtgagcg acaagaccgg
     1141 actggtcgaa ctggccaccg cgctcgccgc agcgggcgtc gagatcgtgt cgaccgggtc
     1201 gacggcgaag acgatccggg atgccgcgat ccccgtcacc gaggtggccg aggtcaccgg
     1261 cttccccgag tccctcgacg gccgcgtgcg cacgctgcat ccggcgatcc acgcgggcct
     1321 cctcgccgac ctgcgactcg cctcgcacga gtccgaactc gccgagctcg gcatcgcgcc
     1381 cttcgagctc gtggtgtcga acctgtaccc gttcgtcgag accgtggcat ccggtgccgc
     1441 cgtgaatgac gtgatcgagc agatcgacat cggcggaccg gcgctggtgc gcgcctcggc
     1501 caagaaccac gcgaatgttg cgatcgtgac atcccccgct tcgtatcccc aggtgctgaa
     1561 ggcgctgacc ctcggcggca cgaccctcgc gcagcgccag cgcctcgcgg gggaggcgtt
     1621 cgcgcacacg gcggcgtacg acaccgcggt ggcggcgtac ttcgcggcga acgtcggcat
     1681 caagaacccg gtggtcgaga ccccggtcgc tgaggcactc gaagcgacct tcccccagac
     1741 cctcacctac gaggtcgagc tctcgaccgg cctgcgctac ggcgagaact cccaccagca
     1801 ggcggcgctc tacgtcgagc ccgccggccg cggcatcgcc caggccaccc agctgcaggg
     1861 caaggagatg tcgtacaaca actacgtcga cgccgatgcc gccctgcgcg ccgcgtacga
     1921 cttcgacgaa cccgcggtgg cgatcatcaa gcacgccaac ccctgcggca tcgcggtggc
     1981 gcgcggcacg ggcgatccga tcgcctcggc gcacgctcgc gcgcacgcgt gcgacccggt
     2041 ctccgcatac ggcggcgtca tcgcggccaa ccgcaccctc acactcgagg ccgcggagtc
     2101 gatcaaggac atcttcaccg aggtgatcgt cgcgcccgca ttcgagccgg cagcgctcga
     2161 gctactcgca tcgaagaaga acctgcggct gctgcaactg cccgcgggct acggtcgctc
     2221 cgacaccgag ttccgccaga tctcgggcgg tgcgctcgtg cagacccccg acacctacgt
     2281 cgacttcagc tcggatgcct ggactctcgt cgcaggaccg gaagccgacg acgccacccg
     2341 cgccgacctc gagttcgcat ggcgggcggt gcgcgccgtg aagtcgaacg cgatcctgct
     2401 ggcgaaggac ggagcatccg tcggggtggg catgggccag gtcaaccgcg tcgactcgtg
     2461 cggcctcgct gtctcgcggg cggccgaccg cgcgatcgga tcggtcgcgg cgagcgacgc
     2521 gttcttcccg ttcgccgacg ggcttgagat cctgctcgag gcgggcgtac gcgcgatcgt
     2581 gcagcccggc gggtcgatcc gcgacgagga ggtcatcgcc gccgcgcagg atgcgggcgc
     2641 cacgatgtac ttcacgggcg agcggcactt cttccactga cgtcggccgg gtctcgacag
     2701 gctcgaccac cggtcccgac tagcggtcgc cttgccatgc ccgaacccga ggcatagtct
     2761 gtaaggctgc ccgggcggcc caccggcacc cgcgcagctc cccacagccc tgcgacccag
     2821 gaattcacca tgtctgcacc aacggccgaa cccggccgcg ctgataccgt cggaaccttc
     2881 gcggccatcc gacggctcta cccgtacgcg aaacccgcca tgccgcgcat ctacctcggc
     2941 atgctgtcgg cgctgctcgc cgggctcgtg gcgctgctga tcccgcaggt gctgaagtcc
     3001 ctcgtcgacg gaccactgca gtcgggcgac tcggcccaga tctggcctgc gttccttgtg
     3061 gtgctcgggc tgggcatcct cgaggcgatc atgatcgcgc tacgccggtt ctgggtgctg
     3121 acccccggaa cccacatcga agcgcggatg cgcaacgcgc tctacatgaa gctccagacc
     3181 ctgccggtga gcttccacga tcgctggccc agcgggcagc tgctgtcgcg ggccgtgagc
     3241 gacctgaacc tgatccgccg ctggatctcg ttcgggctcg ttctgctggt cgtcaacgtg
     3301 ctgacgatcc tcgtgggctt cgccttcctc gtgaacatct cgtggatcct cggcgtgctg
     3361 ttcatcgtgt gctcgatccc cttgtgggtt tacggctatc tgttcgagaa gcgctactcg
     3421 atcgtcgccc gccgcagcca ggatcaggcc ggagacctcg cgacgaccgt cgaggagtcg
     3481 gtgcacggca tccgggtgct gaaggcgttc gggcgcggca agcacgcgct cgagaacttc
     3541 gccacccagg cggaggaact gcgcggcacc gagatcgaga aggccaaggc gatcgcgggc
     3601 atctggctgt ggctgcttct cgtgcccgac gtgaccttcg ctctcgcgct gctgggcggg
     3661 gtgtggctga cggcgaccgg gcagatctcg gcgggcgacc ttgtcgcgtt cttcgccacc
     3721 gcgacggtgc tgcgcttccc ggtggagtcg atcggcttcc tgctgtcgat gaccttcgac
     3781 acccgcacgg ccgccgaccg tttcttcgag gtgatggatg cgcccaacgc gatcgtcgac
     3841 ccggagcacc cgaagaccat cgagcacccc gagggacgcc tggtgttcac cgacgtgcac
     3901 ttccgctacc cggactcacc cgagcgcgtg cccgacctgg tcgacggcgt ctcgctcgcg
     3961 atcgagccgg gggagaccat ggccctggtc gggctcaccg gctcgggcaa gtcgacgctg
     4021 acggcgctca cgacacgcct ctacgacatc gacagcgggt cgatcgccat cgacggcgtc
     4081 gacatccgcg acctcacgct cggtgagttg cgcacccaca tcgcgatggc cttcgaggat
     4141 gcgacgctgt tctcggcgac cgtgcgcgac aacgtgctcc tgggccgacc ggatgccacg
     4201 gaagccgatc tggcggaggc cctcgacatc gcacaggccg ccttcgtgca cgagctgccc
     4261 gacgggctgg acaccgcagt gggggaggag ggcctcagcc tcagcggcgg acagcgccag
     4321 cggctcgccc tcgcccgggc ggtggccgcg aagccctcga tactcgtgct cgacgacccg
     4381 ctctcggcgc tcgacgtcga caccgaggcc ctcgtcgagg ccgcgcttcg cagggtgctc
     4441 gccagcacca cagcgctcat cgttgcgcac cgcccgtcga cggtgatgct cgccgaccgt
     4501 gtcgccctgc tcgagcacgg ccgggtcacc gccgtcggca cccactccga gttgctcgcc
     4561 tcgagcgagc actaccgctt cgtgatctcg tccctggagg acgaacagcg cagggaaagg
     4621 gctcaggcat gagcgccgta tccggtgtcg gcggcgagga gcgcaccgac tacagcaaga
     4681 cggagagccg tcagatccgg cagcggtcgt tgcggctgct cggctcgctc gtgctgccgg
     4741 tgcgggcccg cgtcatcctg accatggtgg tcgtcgtcgt gtcgacggcg gcgcaggtgg
     4801 ccggccccct gttcatcgcc tacggcatcg acacggggct gcccgcgctg ctgaaccagg
     4861 actggatgcc gctcgccggc gccgtcacgg cctacctcgc ggcgggcatc ctcggggcgg
     4921 cactcatcgc gtggtacacc gtgctgacgg cccgcatcag ccaggcgatc ctgttcgacc
     4981 tgcgcaagcg cgtgttcctg cacacccagc ggttgtcgct ggagttccac gagagctaca
     5041 cctcggggcg catcatcgcg cgccagacca gcgacctcga cgcgatccgc gagctgctcg
     5101 actccggtat caaccagctc gtgcagggcc tgctctacat ggtgttcatc gcgatcgcca
     5161 tgttcaccct cgatggcctg agcgggctca tcctggtggc ctcgctcgtt ccgcttctgc
     5221 tgctcacccg gtggttccag gtgcgctcgc agcgcttctt ccgcaccacg cgtaccgcct
     5281 cggcgacgct gatcgtgaag ttcgtcgaga ccatgacggg catccgcgcg gtcaaggcgt
     5341 tccgcaccga ggagagcaac cgcaccgtgt tcggcggtct cgtggagaac taccggcgca
     5401 ccaatcagcg cgtcatccag ctgttcggag tcttcgaccc cgggctcgtg ctcatcggca
     5461 acgtgacgct cgcggtgatc ctgctcgtgg gcgggctccg ggtcgcgacc ggtggcatcg
     5521 ccatcggcgt gctgctcgcg gcgctgctct acgcgcgcca gttcttcgcc cccgcgcagg
     5581 agatggcgat gttctacaac tcctaccagt cggcagcggc cgccctggag aagatctcgg
     5641 gagtgctcga ggaacagccg agtgtgcccg accccaccaa gcccgtcgac ctgctcaaag
     5701 cgcagggtcg ggtgaccttc gatgccgtga ccttctccta caaagacgac cgggtcgtgc
     5761 tgccgaacct cgagctcgag attccggccg gccagaccat tgccctggtc ggcaccacgg
     5821 gggcgggcaa gtcgactctc gccaagctca tctcccggtt ctacgacccg acc
//
LOCUS       DAOSOU010000128         5852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13795_length_5852_cov_7.057271, whole genome shotgun sequence.
ACCESSION   DAOSOU010000128 DAOSOU010000000
VERSION     DAOSOU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13795_length_5852_cov_7.057271"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PLW15_07760"
     CDS             <1..1063
                     /locus_tag="PLW15_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOT34324.1"
                     /translation="APTADTAPPPPLAAPRPQRTGLGANAKPAAASPAAATPPPPAAP
                     APAAGTTSSRRGFRLPESTVARWVLGGVALIVLAAIVVLIARWLVGLDGVKDFLTTYP
                     GETELPETAPVGIPTWLAWQHFFNVFFMVLIVKTGWQVRTQKRPPASWTPKWQPGGRK
                     ISLTLWTHQSLDLLWLTNGLIYIVLLFATGHWLRIVPTSWEVVPNALSAALQYASLDW
                     PTENGWVNYNSLQLIAYFATVFIAAPLAIITGVRMSGLWPKKAPRLNKIYPIEIARAI
                     HLPVMFYFVAFTIVHVTLVLATGALRNLNHMFGGQDAVNWLGFIIFVVSLLVIVGGWF
                     AARPIVVAPIAGLFGKVGR"
     gene            complement(1089..2099)
                     /gene="fgd"
                     /locus_tag="PLW15_07765"
     CDS             complement(1089..2099)
                     /gene="fgd"
                     /locus_tag="PLW15_07765"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HOT34325.1"
                     /translation="MTLPLRFGYKASAEQFAPTELLNYAVLAEQAGFDSVFVSDHLQP
                     WNHEGGHAPAAMPWLGALGARTERIIMGTSVLTPTFRYHPGVVAQAFGTLGVLFPGRV
                     ILGVGTGEALNEVALGIPWPDVKERFARLKEALELIDLLWREDRVTFDGQYFQTENAT
                     IYDRPEVPVPVYIGASGPAATRLAGRVASGFITTSGKARELYTETLLPALAEGLEKGG
                     RTADDVDTLMEMKVSFDHDRERALQDTRYWAPLALSPEEKMGVEDPQEMQRLADALPI
                     ERAASRWIVSTDPDEHVSRVREYIDLGFRHLVFHGPGADQERFIKLYGEEILPRLRAL
                     DA"
     gene            complement(2096..>2950)
                     /locus_tag="PLW15_07770"
     CDS             complement(2096..>2950)
                     /locus_tag="PLW15_07770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOT34326.1"
                     /translation="NLASTWPVHIIERLEIARRAAARGIPLVITGQTLGPGLVPDDER
                     MLAELLHSAALVGVRESASHALASRLGVDDGRLRQGADDASFLGLPPGEHPAPEPYVL
                     VSLSTHVGAADRTDFVDSVAALLDSIARVSGVEIAFLAHFGPLAPPWQRGDVVMHEAV
                     AARMTQPTRTEPTSTAAEAARLARSASMVVTSRYHPAVFAGPSGVATLGIAVDDYTTV
                     KLSGALAAFGQASVVAAADLADAPQLAERLWGARHEIRVRGQVIAAERRAESDAWWDR
                     VVGVVSNT"
     assembly_gap    2951..3050
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3149..5299
                     /locus_tag="PLW15_07775"
     CDS             3149..5299
                     /locus_tag="PLW15_07775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34327.1"
                     /translation="MGIQTSLDAVQQAERLIDALRLADDLAFEASRDGGQRTIRVLQS
                     ALKGDDQVAAIAATHALAQIFDDEAEAVLVRLLSSKRTYLREHAAWALSSRLPRFDAV
                     ARLIRLVAAGEFAGMLAQRTLEKWIAATPDHIAVGLESALLGIDEPGPRARIVETLGL
                     VRSRISGAPLMAIARDPGEADEVRVAAASAIGQRVGSGESEELLLALAAGDGPVADAA
                     RLGLVDIAAERHNTRRGAGLTVAQLFLHADIDPDLAQAGSGDNGGIATLLVRLGDALV
                     ARPADTEYPVDRVVTLSRGSVESALDSLEELRLGGAGHSYGRVPLMVEPVQSASAWPL
                     RVTARRGIRRVLRAAGPVDIIHLRMADVGSLAAVDAARELNIPAVFTVAPDPHAVIAS
                     MSLSGALNRTNFGAVDEREHFWFRTRLVQRIAANAAHTVLFPRPELKRDMLSLVGIDI
                     SAHPERHTIVPEGIDLGVVDRAVAEATAAVETPARAQLTALLEQLPPERRGLPLIVSV
                     GRFHRVKGMATIVEAWAGGALKDRANLLLIGGNLDAPSADEREQLDLMDAIVPADGRA
                     ALGLLLPGHQPNDTVARWVAAVRRGIPGLAAPNGIYVCGSLKEEFGIALLEAMASGLI
                     VVAPNGGGPATYVEQGVTGYLTSTWHVGMLAEAMTEALDAATAETSDDRAERSRRTVE
                     TSFTIQAMAGSLAAVYAYVHEQEIEHHAEPVSAP"
     gene            5296..>5852
                     /locus_tag="PLW15_07780"
     CDS             5296..>5852
                     /locus_tag="PLW15_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34328.1"
                     /translation="MTLLIISPDYASHLLPLATLGTAWRDAGERVVVATGPATASIVE
                     SFGFERVNLQLGKGSNPGVIRAEDQPRGEDDALRGFFDATRQGMIATLAFQAEARLSD
                     LMWEPVRVAREVLAVVASVRPDSIIVDHLAFSARLALVAGGVAYADVVLGHPTALPVG
                     DEVYGYPPEWPAAFSPSVDELAALR"
BASE COUNT          824 a         2028 c         2025 g          875 t
ORIGIN      
        1 cgcacccacc gcggacactg ccccgccgcc accgctcgcc gccccgcgcc cgcagcgcac
       61 cgggctcggc gctaacgcga agcccgccgc cgctagtccc gccgccgcaa ccccgccgcc
      121 gccggccgca cctgcgcccg cggcaggaac gacctcgtcc cgccgcggat tccgactccc
      181 cgagagcacc gtcgcgcgct gggttctcgg cggcgtggca ctcatcgttc tcgcagctat
      241 cgtcgtactc atcgcccgct ggctcgtcgg tctcgacggg gtgaaggact tcctcaccac
      301 ctaccccggc gagaccgagc tgcccgagac ggcaccggtg ggcatcccca cctggctggc
      361 ctggcagcac ttcttcaacg tcttcttcat ggtgctgatc gtgaagaccg gctggcaggt
      421 gcgcacgcag aagcggccac cggcatcctg gacccccaag tggcagccgg gcggtcgcaa
      481 gatcagcctc accctctgga cgcaccagtc cctcgacctg ctctggctga ccaacggact
      541 gatctacatc gtgctgctgt tcgccaccgg gcactggctg cggatcgtgc ccacgtcgtg
      601 ggaggtcgtg ccgaacgccc tgtcagcggc gctgcagtac gcctcgctgg actggcccac
      661 cgagaacggc tgggtcaact acaacagcct ccagctgatc gcgtacttcg cgacggtctt
      721 catcgcggcg ccgctggcga tcatcaccgg cgttcgcatg tcgggcctgt ggccgaagaa
      781 ggccccgcgc ctgaacaaga tctacccgat cgagatcgcc cgggccatcc acctgccggt
      841 catgttctac ttcgtcgcct tcacgatcgt gcacgtcacg ctcgtgctgg ccaccggggc
      901 gttgcgcaac ctcaaccaca tgttcggtgg tcaggatgcc gtcaactggc tcggattcat
      961 catcttcgtg gtgtcgctgc tggtgatcgt cggcggctgg ttcgccgcgc gtccgatcgt
     1021 cgtcgcgccg atcgccgggc tgttcggcaa ggtcgggcgc taggcggcat ccgggtcagg
     1081 ccatccggtc aggcgtcgag cgcgcgcagg cgcggcagga tctcctcgcc gtagagcttg
     1141 atgaagcgct cctggtctgc gccggggccg tggaacacga ggtgccggaa gcccaggtcg
     1201 atgtactcgc gcacgcgcga cacgtgctca tcggggtccg tcgagacgat ccagcgtgag
     1261 gccgcccgct cgatcggcag ggcgtcggcg aggcgctgca tctcctgcgg gtcctcgacg
     1321 cccatcttct cctcggggct gagggccaga ggcgcccagt accgggtgtc ctgcagcgcg
     1381 cgctcgcggt cgtggtcgaa tgacaccttc atctccatga gggtgtcgac gtcgtcagcc
     1441 gtgcggccgc ccttctcgag tccctcggcc agggccggca gcagggtctc ggtgtagagc
     1501 tcgcgggcct tgcccgaggt cgtgatgaat ccggatgcca cccgccccgc caaccgcgta
     1561 gcggccgggc ctgacgcgcc gatgtacacc ggcacgggca cctcggggcg gtcgtagatc
     1621 gtcgcgttct cggtctggaa gtactggcca tcgaacgtga cccggtcttc gcgccagagc
     1681 aggtcgatga gctcgagcgc ctccttgagg cgggcgaagc gctccttgac gtcgggccac
     1741 gggatgccga gggcgacctc gttgagcgcc tcgccggttc cgacgcccag gatgacgcgg
     1801 cccgggaaca gcaccccgag agtgccgaac gcctgagcga ccacgccggg gtggtaccgg
     1861 aacgtcggcg tgagtaccga ggtgcccatg atgatgcgtt cggtgcgtgc gccgagagcg
     1921 ccgagccacg gcatcgcggc gggtgcgtgg ccgccctcgt ggttccaggg ctggaggtgg
     1981 tcggagacga atacggagtc gaaaccggcc tgctcggcca gcacggcgta gttcagcaat
     2041 tcggtggggg cgaactgctc ggcggaggcc ttgtagccga agcgcagggg gagagtcatg
     2101 tattcgagac tacgccgacc acgcggtccc accacgcgtc tgattccgct cgtcgctcgg
     2161 ccgcgatcac ctgaccgcgc acgcggatct cgtgacgggc gccccagagc cgctcggcga
     2221 gctgcggggc atcggcgagg tcggcggcgg cgacgacgct cgcctgcccg aatgcggcca
     2281 gcgcgccgct cagcttgact gtcgtgtagt cgtcgacggc gatccccagg gtcgcgaccc
     2341 ccgacgggcc tgcgaagacc gcggggtggt agcggctggt gacgaccatg gaggcggagc
     2401 gcgcgagccg ggcggcctcg gccgcggtgc tcgtcggttc ggtgcgggtg ggctgcgtca
     2461 tccgggccgc gaccgcctcg tgcatcacga cgtcgccgcg ctgccagggt ggcgcgagcg
     2521 gaccgaagtg ggcgaggaag gcgatctcga cgccgctgac gcgtgcgatc gagtcgagga
     2581 gggccgcgac tgagtccacg aagtcggtgc gatcggcagc gccgacgtgg gttgagagtg
     2641 acacgagcac gtagggttcg ggcgcgggat gctcgcccgg aggcagaccg aggaagctcg
     2701 cgtcatccgc gccctgtcgc agccgaccgt cgtcgacgcc gagccgggac gcgagcgcgt
     2761 gcgacgccga ctcgcgcacc ccgacgagcg ccgccgagtg cagaagctcg gcgagcatcc
     2821 gctcgtcgtc gggcaccagg ccgggcccga gggtctgtcc ggtgatgacg agcgggatgc
     2881 ctcgcgccgc ggcacggcgg gcgatctcga gccgctcgat gatgtgcacg ggccaggtcg
     2941 aggcgaggtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caacgccgat
     3061 atcaccgatg gccagaattc gcaccccccg agtgaatcac accgctggtg acatacagtg
     3121 atgagggccg atcgagaggg taattcaggt gggaatccag acgtcactcg acgctgtgca
     3181 gcaggccgag cgcctgatcg acgcgctgcg gctcgctgac gatctcgcgt tcgaggccag
     3241 ccgggatggc ggccagcgca cgattcgcgt gctccagagt gcgctcaagg gcgacgacca
     3301 ggtcgcggcg atcgcggcga cccatgcgct cgcccagatc ttcgacgacg aggccgaggc
     3361 ggtgctcgtg cggctgctct cgagcaagcg cacctacctt cgggagcacg ccgcgtgggc
     3421 gctctcgagc aggctgccgc ggttcgacgc ggtcgcccgt ctcatcaggc tggtggccgc
     3481 gggtgagttc gcgggcatgc tcgcgcaacg cacgctggag aagtggatcg ccgcgactcc
     3541 cgatcacatt gcggtcggcc tcgaatccgc cctgctcgga atcgacgagc cgggcccccg
     3601 tgcgcgcatc gtcgagaccc tcggcctggt gcgctcgcgc atctcgggcg ccccgctgat
     3661 ggccatcgcg agggaccccg gcgaggccga cgaggtgcgc gtcgccgcgg cctcggcgat
     3721 cggccagcgg gtaggttcgg gcgagtctga ggagctgctg ctggcgctag ccgccggcga
     3781 cggcccggtc gcggatgccg cgcgcctcgg tctcgtcgac atcgccgccg agcgccacaa
     3841 cacccgtcgc ggcgcgggac tcaccgtggc ccagctgttc ctccacgccg acatcgaccc
     3901 cgatctcgcg caggcgggaa gcggcgacaa cggcggcatc gccaccctgc tcgtgcggct
     3961 cggcgatgcg ctcgtcgctc gcccggccga cacggaatac cccgtggatc gggtggtcac
     4021 gctgtcgcgg gggtcagtcg agtccgcgct cgacagcctc gaggagttgc gtctcggtgg
     4081 tgctgggcac tcgtacggtc gggttccgct catggtcgag cccgtgcagt ccgccagtgc
     4141 gtggccgctg cgcgtgacgg cgaggcgagg catccggcgg gtgctgaggg cggccgggcc
     4201 cgtcgacatc attcacctgc gcatggccga tgtgggcagc cttgccgctg tcgatgcggc
     4261 gcgcgaactc aacattcccg cggtgttcac cgtggctccc gacccgcacg ccgtcatcgc
     4321 gtcgatgagc ctgtccggtg ccctgaaccg caccaacttc ggcgccgtcg acgagcggga
     4381 gcacttctgg ttccgcaccc ggctggtgca gcgcatcgcc gccaatgcgg cccacacggt
     4441 gctcttcccg cggcccgagc tcaagcgcga catgctgagc cttgtgggca tcgacatcag
     4501 cgcccacccc gaacggcaca cgatcgttcc cgagggcatc gacctgggcg tcgtcgaccg
     4561 cgccgttgcc gaggccaccg ctgcggtcga gaccccagcg cgagcccagc tcaccgccct
     4621 gctcgagcag ctgccgcccg agcggcgcgg gctgccgctc atcgtcagcg tcggacgctt
     4681 ccaccgcgtc aagggcatgg cgacgatcgt cgaggcgtgg gcgggtggcg cgctcaagga
     4741 tcgcgccaac ctgctcctca ttggcggcaa cctcgacgcg ccctcggccg atgagcgcga
     4801 acagctcgat ctcatggacg cgatcgttcc cgccgacggg cgagcagcac tgggcctgct
     4861 gctgccggga caccagccca atgacacggt cgcgcgctgg gtggcggccg tgcgtcgggg
     4921 catccccggc cttgccgccc cgaacggcat ctacgtctgc ggaagcctca aggaggagtt
     4981 cggcatcgcc ctgctcgagg cgatggcgag cggcctcatc gtggtcgccc ccaacggcgg
     5041 cggccccgcg acctacgtcg agcagggggt taccgggtac ctcacgagca cgtggcacgt
     5101 cggcatgctc gccgaggcga tgaccgaggc actggatgcc gcgaccgccg aaacctccga
     5161 cgaccgggcc gagcgttccc gccgcaccgt ggagacgagt ttcaccatcc aggcgatggc
     5221 gggctcgctc gccgccgtct acgcctacgt gcacgagcag gagatcgagc accacgccga
     5281 gccggtgtcg gcgccgtgac gctgctcatc atcagcccgg attacgcctc acaccttctg
     5341 cctttggcaa ccctcggcac cgcatggcgc gacgcagggg agcgcgtggt ggtcgccacg
     5401 ggtcccgcta ccgcgtcgat cgtcgagtcc ttcggctttg agcgggtgaa cctgcagctg
     5461 ggtaaggggt cgaacccggg cgtcatccgc gccgaggacc agccgcgcgg ggaggacgac
     5521 gccctgcggg gattcttcga cgcgactcgg cagggcatga tcgccacgct ggccttccag
     5581 gccgaagccc ggctcagcga cctgatgtgg gagccggtgc gggtcgcccg cgaggtgctc
     5641 gcggtggtgg catcggtgcg tcccgactcg atcatcgtcg accacctcgc gttcagcgca
     5701 cggctggctc tggtcgccgg gggtgtggcc tacgcggatg tcgtgctcgg gcatccgacg
     5761 gccctgccgg tcggcgacga ggtatacggg tacccaccgg agtggccggc ggcgttctcg
     5821 ccgagcgtcg acgaactcgc ggccttgcgc ga
//
LOCUS       DAOSOU010000129         5843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13821_length_5843_cov_5.584485, whole genome shotgun sequence.
ACCESSION   DAOSOU010000129 DAOSOU010000000
VERSION     DAOSOU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13821_length_5843_cov_5.584485"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..597)
                     /gene="greA"
                     /locus_tag="PLW15_07785"
     CDS             complement(109..597)
                     /gene="greA"
                     /locus_tag="PLW15_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT34329.1"
                     /translation="MATDSSVTWLTQEAFDRLTAELDQLAGEGRTEIAKKIEAAREEG
                     DLKENGGYHAAKEEQGKIEARIRVLTELLRHSVVGEAPESTGVVAPGTLITAAILGTE
                     RTFLIGSREIVGDSDIEVYSEGSPLGTAIIGMKVGDETSYTAPNGKSIDVKIVKVETY
                     VP"
     gene            complement(707..1132)
                     /locus_tag="PLW15_07790"
     CDS             complement(707..1132)
                     /locus_tag="PLW15_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HOT34330.1"
                     /translation="MSHPESPLAPESAELAARYGRTPSAKRRTRWVVVASALAFVAVF
                     TAWVVWGGLLSPAAQLETEDIGHTILSDSEVSVSWTVNVEPGTATKCAVQALNSTFAI
                     VGWVIVDVAASSERTRTFTKTVRTTELAVTGLIYRCWLT"
     gene            1188..>1746
                     /locus_tag="PLW15_07795"
     CDS             1188..>1746
                     /locus_tag="PLW15_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT34331.1"
                     /translation="MARRLLAVHAHPDDESSKGAATYAHYRSLGDEVLVVSCTGGERG
                     DVQNQGLPANELAMAERDMAGLRRIEMSRAAAAIDIAHRWLGYEDSGLPEGDEPLPAN
                     CFARIPLQVSAEPLVGIIREFRPHVLVTYDEQGGYPHPDHIRTHELSMFAWRAAADAR
                     QYPQQGPAWHVPKVYYDRIFNSHRLE"
     assembly_gap    1747..1846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1847..2141
                     /locus_tag="PLW15_07800"
     CDS             <1847..2141
                     /locus_tag="PLW15_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34332.1"
                     /translation="HRGHPMITLLAWLADVTPTPSPSPALNEDLVTPGIVGFVVTFFI
                     AVATVLLVIDMTRRIRRVRYRAEVQEQLEAEEREGAEGGKAGKPGKATPAPED"
     gene            complement(2119..2877)
                     /locus_tag="PLW15_07805"
     CDS             complement(2119..2877)
                     /locus_tag="PLW15_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOT34333.1"
                     /translation="MTAHDTGTAPDSTPAPAHDDEAELPNIPLLEDSIEYEALDVRPT
                     WRGWIHAGTFPLAIVLGVILIVFADGVPAKVSSAIFVASSLLLFGISALYHRFTWSER
                     ARVLLKRFDHSNIFLLIAGSYTPITVLCLPTPKAILLLSLVWGGALLGIGFRIFWIGA
                     PRWLYVPIYVLLGWAAMMFIVDFFQASWIMMTLIIVGGLCYTAGAVIYGLKRPNPFPG
                     KFGFHEIFHTLTLLAFLCHWTGIFIVAINPPVLA"
     gene            2942..3727
                     /locus_tag="PLW15_07810"
     CDS             2942..3727
                     /locus_tag="PLW15_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOT34334.1"
                     /translation="MSKRQEPLGRGLLYGLYQNRLRRSLVPEELPHHVAMIIDGNRRW
                     ARLRELETAAHGHRAGAEKMHEFLEWCDGLGIRVATLYLLSTDNLTGRSKDELEGLFE
                     IIGDLAEDLSHFRDWRVQHVGSTDGLPAALVSKLLDAQTRTAGNTGLHINLAIGYGGR
                     REIADAMRSIVRTHESDGGSLDALAELLTPELIGEHLYTGGQPDPDLVIRTSGEQRIS
                     DFMLWQSAHSEFYFMEALGPDLREVDFLRALRDFARRQRRFGQ"
     gene            3939..5303
                     /locus_tag="PLW15_07815"
     CDS             3939..5303
                     /locus_tag="PLW15_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT34335.1"
                     /translation="MTRPNQKLNGSQSGRSARSESSAAQAERTYVLDTSVLLSDPKAA
                     FRFAEHHVVLPVVVITELEAKRNDPEIGYFARQALRNLDELRVQHERLDFPIAVGDGG
                     SLRVELNHSNMSVLPSGLQLGDNDSRILAVALNLANEGLAVTVVSKDLPLRVKAASIG
                     LAAEEYRAELAVDSGWTGQKDIDLSSEQMAQLYDNEQLHSRLVEDLPVNTGLIIHSDR
                     GSALGRVSGRGEMKLVRGDRDIFGLHGRSAEQRLAIDLLLDPEIGIVSLGGRAGTGKS
                     ALALCAGLEAVLEKQQHRKIMVFRPLYAVGGQELGFLPGDAAEKMNPWAQAVFDTLGS
                     VVSQNVLDEVLERGLLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRIGQNS
                     RVVLTHDVAQRDNLRVGRHDGVASVIETLKGHALFGHITLTRSERSAIAALVTEMLES
                     NELA"
     gene            5404..>5843
                     /locus_tag="PLW15_07820"
     CDS             5404..>5843
                     /locus_tag="PLW15_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34336.1"
                     /translation="MSWVFGALAVTLGLILLWGVFAPRSQWRILAGWSVSDPHLHEPG
                     GASYGLRRLLSGIGALTLLGIVGVTSASAVAGLPSESPRPSLVSQLWGRPDPSVVNRM
                     IVPLSGPPSGLVEMPVLAYEDVSDDLPDYFDRLHNFTLLGNGDAP"
BASE COUNT          964 a         1888 c         1927 g          964 t
ORIGIN      
        1 cgcgcatcga cttctaggtt ggttgagtag cgccgaaggc gcgtatcgaa accactccga
       61 cgcatcggtt tcgatatgcc gcttcgcggc tactcaaccg gcagcaactc agggaacgta
      121 ggtctcgacc ttgacgatct tcacgtcgat cgacttgccg ttgggcgccg tgtagctcgt
      181 ctcgtcgccg accttcatgc cgatgatcgc ggtgccgagc gggctgccct cgctgtagac
      241 ctcgatgtcg ctgtcaccca cgatctcgcg tgagccgatg aggaacgtgc gctcggtgcc
      301 gaggatcgct gcggtgatga gcgttcccgg cgcgaccaca ccggtcgatt cgggggcctc
      361 gcccaccacg gagtgacgca gcagctcggt cagcacgcgg atgcgcgcct cgatcttgcc
      421 ctgctcctcc ttggcagcgt ggtagccgcc gttctccttg aggtcgccct cctcgcgagc
      481 ggcctcgatc ttcttcgcaa tctccgtgcg accctcgccg gcgagctggt cgagctccgc
      541 agtcagtcga tcgaacgctt cctgggtcag ccacgtgacc gagctgtctg tagccacagt
      601 gtctcctaac gaaatgaacc ggccgttgcg ggccggttcc ggatgatccc gccgcactga
      661 gcaacactga atgctgatca ggcgggcggg tgcgagtccg tcaagcctag gtgagccagc
      721 aacggtagat caacccggtt acggcgagct cggtcgtgcg caccgtcttc gtgaacgtgc
      781 gcgtgcgctc gctcgaggcg gcgacatcca cgatcaccca cccgacgatc gcgaaggtcg
      841 agttgagggc ctgcacggca cacttcgtcg ccgttcccgg ctcgacattg acggtccagc
      901 tgaccgacac ctcgctgtcg ctgagaatcg tgtgaccgat gtcttcggtc tcgagctgcg
      961 ccgccggact cagcagcccg ccccacacga cccacgccgt gaacacggca acgaaggcga
     1021 gggcggatgc cacgaccacc caccgggtgc gtcgcttcgc gctcggcgtg cgcccgtagc
     1081 gcgccgcaag ctccgccgac tcgggggcga gtggggactc agggtgcgac acgcatggct
     1141 ccgttaggct cggtagttca ggaccattct ttcacccaag gagcacggtg gcacggcgct
     1201 tgctagcagt gcacgcccat cccgacgacg agtcgagcaa gggcgcggcc acgtacgcgc
     1261 actaccgcag cctgggtgac gaggttctgg tggtcagttg cactggcggc gaacgcggcg
     1321 acgtgcagaa ccagggtctg ccggcgaacg agctggcgat ggccgagcgc gacatggccg
     1381 ggctccgtcg catcgagatg agccgcgcgg cggcagcgat cgacatcgca caccgctggc
     1441 tcggctacga ggattcgggg ctgcccgagg gtgacgagcc gttgccggcg aactgctttg
     1501 cgcgcatccc gcttcaggtc agtgccgagc cgctggtggg catcatccgt gagttccgac
     1561 cgcatgtgct ggtgacctac gacgagcagg gtgggtaccc gcaccccgac cacattcgca
     1621 ctcacgagct gtcgatgttc gcctggcgtg cggcggcgga tgcccggcag tacccgcagc
     1681 agggcccggc ctggcacgtt ccgaaggtct actacgaccg catcttcaac tcccaccgtc
     1741 tcgagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacac cgaggacacc
     1861 cgatgatcac cctgctcgcc tggctcgccg acgtcacccc gacgccgagt ccgtcacccg
     1921 ctctcaacga ggacctggtc acgccgggca tcgtcgggtt cgtcgtgacg ttcttcatcg
     1981 cggtggcgac ggtgctgctc gtgatcgaca tgacacgtcg catccgtcgg gtgcgctacc
     2041 gcgccgaggt tcaggagcag ctggaggccg aggagcgcga aggcgcagaa ggcggcaagg
     2101 cgggcaagcc cggaaaggct acgccagcac cggaggattg atcgccacga tgaagatgcc
     2161 ggtccagtgg cagaggaacg ccagcagggt cagcgtgtgg aagatctcgt ggaagccgaa
     2221 cttgccgggg aacgggttgg ggcgcttgag cccgtagatg acggcgccgg cggtgtagca
     2281 gaggccaccc acgatgatga gcgtcatcat gatccagctg gcctggaaga agtcgacgat
     2341 gaacatcatc gccgcccagc ccagcagaac gtagatcggc acgtacagcc agcgcggtgc
     2401 gccgatccag aagatccgga agccgatgcc cagcagcgct ccaccccaca ccagcgagag
     2461 cagcaggatg gccttggggg tggggaggca gagcacggtg atgggcgtgt atgagcccgc
     2521 gatcagcagg aagatgttcg agtgatcgaa gcgcttgagc agcacgcggg cgcgctcgct
     2581 ccaggtgaag cggtggtaca gggctgagat gccgaacagc agcagagacg aggccacgaa
     2641 gatcgcggag ctcaccttcg cgggtactcc gtcggcgaag acgatgagga tgacgcccag
     2701 cacgatcgcc agggggaagg ttccggcgtg gatccagccg cgccacgtcg gcctcacgtc
     2761 gagtgcctcg tactcgatgg aatcctcgag gagggggatg ttgggcagct cggcctcgtc
     2821 gtcatgcgcc ggtgcgggtg tcgagtcggg tgccgttccc gtgtcgtgcg cggtcatgtc
     2881 gccagtgtac gcgcgggata tcacagcaag ctggcagcac cgggtgggct agcgtaggcg
     2941 tgtgagtaag cgccaggaac ccctcgggcg cggcctgctc tacggtctct accagaatcg
     3001 cctccgacgc tcactggtgc ccgaggagct gccgcaccac gtcgccatga tcatcgacgg
     3061 caaccggcgc tgggctcgcc tgcgagaact cgagacggca gcccacggac atcgcgccgg
     3121 ggccgagaag atgcacgagt tcctcgagtg gtgcgacggc ctcggcatcc gtgtcgcgac
     3181 cctctacctg ctctccactg acaacctgac cggtcggtcg aaggatgaac tcgaggggct
     3241 cttcgagatc atcggcgacc tcgcggagga cctctcgcac ttcagggact ggagggtgca
     3301 gcacgtcggg tcgaccgatg gccttcccgc tgcgctggtg tcgaagctac tggacgcgca
     3361 gacgcgaacc gcgggcaaca ccggcctgca catcaacctc gccatcggct acggcggtcg
     3421 tcgcgagata gcggatgcga tgcgcagcat cgtgcgcacc cacgagtccg acggtgggtc
     3481 gcttgacgca ctggccgaac tcctcacccc ggagctgatc ggcgagcacc tgtacacggg
     3541 tggacagccc gacccggatc tggtcatccg cacctcgggg gagcagcgca tcagcgactt
     3601 catgctctgg cagagtgccc acagcgagtt ctacttcatg gaggccctgg ggcccgatct
     3661 gcgcgaagtg gacttcctgc gggcgctgcg tgacttcgcc cgcaggcagc gtcgattcgg
     3721 ccagtagtcg ttgcccagca attaacctgc cggatacacg acgcgcggtg tgtcggtcgt
     3781 ttcgcgcaac gcgaaacgcc tagcgtcgag ttatcaggca cggcgcctga tcgagacggc
     3841 cacgtaagtt cgtctcgcga tgtgctaaca ccacatggcc gtcttcgcag actggatccg
     3901 ggagcttgac tgccggggat ggagtgcacg tggccgagat gacccgcccg aaccagaagc
     3961 tgaacggatc gcagtcgggg aggtcggcca ggtctgagtc gtccgccgcg caggccgagc
     4021 gcacttatgt gctcgacacc tcggttctcc tgtctgatcc gaaggccgcg ttccgcttcg
     4081 ccgagcacca cgtcgtgctt ccggtcgtcg tcatcaccga gctcgaggcc aagcgcaacg
     4141 atcccgagat cggctacttc gcccgtcagg cgctgcgcaa cctcgacgaa ctgcgcgtgc
     4201 agcacgagcg cctcgacttc ccgatcgccg tcggcgacgg cggttcgctg cgcgtcgaac
     4261 tcaaccactc caacatgtcc gtgctgccgt ccgggctcca gctcggcgac aacgactcgc
     4321 gcatcctcgc ggtcgcgctc aacctcgcca acgaggggct cgcggtcacc gtggtgtcga
     4381 aggacctgcc gctgcgggtg aaggcggcat ccatcggcct ggcggcggag gagtaccgcg
     4441 ctgagctggc cgtcgactcc ggctggaccg gccagaagga catcgacctc agcagcgagc
     4501 agatggctca gctctacgac aacgagcagc tgcactcccg gctcgtcgaa gaccttcccg
     4561 tcaacacggg cctcatcatc cactccgacc gggggtcggc gctcggtcga gtctcgggtc
     4621 gcggcgagat gaagctggtg cgcggcgacc gcgacatctt cggcctgcac ggccgcagcg
     4681 ccgagcagcg cctggcgatc gacctgctgc tcgatccgga gatcggcatc gtgtcgctcg
     4741 gcggccgggc cggcaccggc aagtcggccc tcgcactgtg cgcggggctc gaggccgtgc
     4801 tcgagaagca gcagcaccgc aagatcatgg tgttccgtcc gctgtacgcg gtgggcggcc
     4861 aggagctcgg cttcctgccc ggggatgccg cggagaagat gaacccgtgg gctcaggcgg
     4921 tgttcgatac cctcggctcg gtcgtctcgc agaacgtgct cgatgaggtg ctggagcgcg
     4981 gtctgctcga ggtgctgccg ctcacgcata tccgcggacg ctcgctgcac gacgcgttcg
     5041 tgatcgtcga cgaggcccag tcgctggagc gcaacgtgct gctcaccgtg ctgtcccgca
     5101 tcggacagaa ctcgcgcgtg gtgctcacgc acgatgtggc tcagcgggac aacctgaggg
     5161 tcgggcggca cgatggcgtg gcatccgtca tcgaaaccct caaggggcac gcgctgttcg
     5221 gacacatcac gctcacgcgg tcggagcgct cagcgatcgc cgcgctggtc actgaaatgc
     5281 tggagtcgaa cgagctcgcc tagccccgga atcatccgtt ctgatgattt tttgtcagat
     5341 tgttactggg aaccgggtgt gcactgcccc tgagaggggg tagaccacgt agggtctact
     5401 accgtgagct gggtatttgg ggccctggcg gtcacgttgg ggctcattct tctgtggggt
     5461 gtcttcgcgc cccgaagcca gtggcgaatt ctcgccgggt ggagcgtgag cgacccccac
     5521 ctgcacgagc ccggtggggc gtcgtacggc ctgcgtcggc tgctgagcgg gatcggcgcg
     5581 ctcacgttgc tcgggatcgt cggcgtgacg tcggcgtcgg ccgttgccgg tctgccctcg
     5641 gagtcaccgc gcccgtcgct cgtctcgcag ttgtggggcc gccccgaccc gtcggtggtc
     5701 aaccggatga tcgtgccgct gagcggcccg cccagcggac tcgtcgagat gcccgtgctc
     5761 gcctacgagg acgtcagcga cgacttgccc gactacttcg atcggcttca caacttcacc
     5821 ctgctgggca acggcgacgc gcc
//
LOCUS       DAOSOU010000130         5823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13875_length_5823_cov_7.464112, whole genome shotgun sequence.
ACCESSION   DAOSOU010000130 DAOSOU010000000
VERSION     DAOSOU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13875_length_5823_cov_7.464112"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLW15_07825"
     CDS             <1..628
                     /locus_tag="PLW15_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34337.1"
                     /translation="RAAGDRGVASLHQSVRCRMCGGGSRTEDAIAASDPHAVDPHAAA
                     EPGLAARIRTVLSESAIYGVILVSALIIVTGQKSDTSWDTFLKVFGTILVFWVAHVFA
                     VVVSNLGLAVGGDKSFGALLLYGVRHSSGLLLGALIPLLVILLGAVGVLSDDTAVWAA
                     LWIDAVLLGVIGYLAVARMTPKPWARAVGAAVTALLGVVIMLMKVLIH"
     gene            complement(635..1318)
                     /locus_tag="PLW15_07830"
     CDS             complement(635..1318)
                     /locus_tag="PLW15_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT34338.1"
                     /translation="MSSGAAAYRNQPDEGFDTASGLLNRLVLVAGASNDSGRAVATRL
                     TDAGARVLAVGSDLRRLAEVPAVSRYECDLASPRGVDSLASRIHAEHGPIDGVVHLVG
                     GWRAGRADDDWAWLESRVVETFRNTSRAFFEDLKASPAGRLVMVSSTSVEVPTWSNAN
                     YVAAKSAAEAWARSAASGFAKGGTAASVILAVGSLGPDGTPVGVLADRIVTLWDEPAT
                     ELNGARFTL"
     gene            complement(1311..2702)
                     /locus_tag="PLW15_07835"
     CDS             complement(1311..2702)
                     /locus_tag="PLW15_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HOT34339.1"
                     /translation="MSRVTVTPTRDAIIGEPEVVEDIVGLPAWRRLKSAVSSLRTHQV
                     KDGSIPDATMHGDCRALIATIRADIRELAGHFPHDADYLTALDADFARWAEGGFGEPD
                     FLDSLVAFQPQRDRVDGMRHLVVFPMYTQNGSSDRRVEAVLIEVIWPEFIADLEAGEY
                     SNALFVPIRFLDFTEGYDTNSAVLFPETVAMREIPHFTWGAIFADREAARFRRVVRAA
                     SEITKLDLPEDAAALLDDQALTEETFVMWDLIHDRTHMRGDLPFDPFMIKQRMPYFLY
                     SLEELRCDLTAFREAVRLERAPETDENTRSHAKLVQYAVIFDRIFRFAITGSRVRNYD
                     GLGGQLLFAWLHQHHVLHWTDTQLTIDWAEVPDVVVELSDAINDLYWKSIDRPKIAHW
                     LAAYDLVAGTLTPHPASKWAVRDLALEGLPRELTDAVMDDEFPLSMFYEALSKKMTDV
                     IESTSGITGGLVE"
     gene            2849..3778
                     /locus_tag="PLW15_07840"
     CDS             2849..3778
                     /locus_tag="PLW15_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOT34340.1"
                     /translation="MLGVLVGFAIIAAVILVGYIVGRSGVLGERGGFVLGRLAFYVLA
                     PCLLFTTLADADVHALFSSLLFVSLTAAVVSAGVFVVIARAFWKRPVSETVVGALGSG
                     YVNANNIGIPVAVYVLGDAATSAPVILLQLLLFAPIALTILDVQARGHASIRRILLGP
                     VTNPIILGSLLGVLLSIFQVRLPDAVMEPFHLIGAAAVPVVLISFGMSLHGTRPLAPG
                     SSRRDVILASTLKLTLMPLVAWLVGSFVFGLSGHRLFAVVVLAALPAAQNVFNYAQRY
                     DRGVILARDVVLITTALSLPVLLVIAALLAPAG"
     gene            complement(3947..4783)
                     /locus_tag="PLW15_07845"
     CDS             complement(3947..4783)
                     /locus_tag="PLW15_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT34341.1"
                     /translation="MRVATWNVNSIRSRVGRVVDWAMREDVDVLAMQEIKCTEAQFPF
                     EAFSEAGYEVELHGLSQWNGVAIASRLPLTDVSIGFPSAPGFGKPLDDGTLPQEARAI
                     GATVEGVRLWSLYVPNGRELDNPHYSYKLEWLANLATETRAWLAAEPDLPLALMGDFN
                     IAPFDHDVWDIAAFAGKTHVSQPERDAFAALEAAGLTDALRSRGVEGYTYWDYQQLRF
                     PRNEGMRIDFILGSRAFDALVTDARIIREERKGDAPSDHVPVVVDLALDIEDDDDRPM
                     FL"
     gene            4883..5029
                     /locus_tag="PLW15_07850"
     CDS             4883..5029
                     /locus_tag="PLW15_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34342.1"
                     /translation="MVDHDELLDADAAAADRRDLRRIVVITTISLALVVLASLLLVAS
                     TIHV"
     gene            5064..5615
                     /gene="pyrE"
                     /locus_tag="PLW15_07855"
     CDS             5064..5615
                     /gene="pyrE"
                     /locus_tag="PLW15_07855"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT34343.1"
                     /translation="MTDARQQLIDFIAAEAVFHGDFTLTSGKKATYYVDLRKVSLDHR
                     VAPLIGQVMIDLIADVPDVFAVGGLTMGADPIAAAVLHQGAARGLAYDAFVVRKEPKD
                     HGRGRQVEGPDLAGKRVIVLEDTSTTGGSPLAAIEALKKVGAEIAGVAVVVDRNTGAR
                     ERIEEAGYPYFAAIGLADLGLPG"
     gene            5612..>5823
                     /locus_tag="PLW15_07860"
     CDS             5612..>5823
                     /locus_tag="PLW15_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34344.1"
                     /translation="MTDDRTPDDELDPSDWLASQFGAPEPEVAPPADARPPVTPPQPV
                     VPLTAAPPTPAAPPAAPAPAVPPFAAP"
BASE COUNT          860 a         2007 c         1989 g          967 t
ORIGIN      
        1 ccgcgcagct ggggatcgtg gagttgcgtc actccaccag tctgtcaggt gcaggatgtg
       61 tggtggggga tcaagaacgg aggacgccat cgctgccagt gacccgcacg cggtcgatcc
      121 gcacgctgcg gccgagcccg gacttgccgc gcgcatccgg accgtgctct ccgagtcggc
      181 gatctacggc gtgattctcg tctcggcgct gatcatcgtc acgggccaga agtccgacac
      241 ctcgtgggac accttcctga aggtcttcgg cacgatcctc gtcttctggg tcgcgcacgt
      301 gttcgcggtg gtcgtctcga acctcggact tgcggtcggc ggtgacaagt cgttcggggc
      361 gctcctgctc tacggcgtgc ggcactcgag cgggctgctg ctcggtgcgc tgattccgtt
      421 gctggtgatc cttctcggcg ccgtcggggt gctctcggat gacacagccg tctgggctgc
      481 actgtggatc gacgctgtgc tgctgggcgt gatcggctac ctcgccgtcg cgcgcatgac
      541 cccgaagccc tgggcacgag cggtgggcgc ggccgtaacc gccctactgg gtgtcgtcat
      601 catgctgatg aaggtgctca tccactgagc gtgactacag cgtgaagcgc gccccgttga
      661 gctcggtcgc cggctcgtcc cagagggtga cgatgcggtc ggcgagcacg ccgacaggag
      721 tgccatcggg cccgagtgac cccacggcca gaatcacgga tgccgcggtg ccccccttcg
      781 cgaaccccga cgccgcgctg cgcgcccacg cctccgctgc ggacttcgcc gcgacgtagt
      841 tcgcgttgga ccacgtcggc acctccacgg aggtcgacga caccatcacg agccgccctg
      901 cgggcgaggc cttgaggtcc tcgaagaagg cgcggctcgt gttgcggaac gtctcgacca
      961 cccgcgactc cagccacgcc cagtcgtcat cggcgcggcc cgcgcgccag ccgcccacca
     1021 ggtgcacgac gccatcgatc ggcccgtgct ccgcgtggat gcgggacgcg agactgtcca
     1081 cgccgcgggg gcttgcgagg tcgcactcgt agcgggatac ggcggggacc tcggcgagcc
     1141 ggcgcaggtc ggagccgacg gcgagcaccc gggcaccggc atccgtcaac cgggtggcga
     1201 cggcgcggcc ggagtcgttg gaggcgccgg ccacgagcac gagccggttg agtaggccgg
     1261 aggccgtatc gaaaccctcg tcgggttggt ttcgatacgc ggctgcgccg ctactcaacc
     1321 agccctccgg taatccccga cgtcgactcg atcacgtcgg tcatcttctt cgagagcgcc
     1381 tcgtagaaca tcgacagcgg aaactcgtcg tccatgaccg cgtcggtgag ctcgcgcggg
     1441 agaccctcga gcgccagatc gcgcaccgcc cacttcgacg cggggtgcgg cgtgagcgtg
     1501 cccgcgacga ggtcgtaggc ggcgagccag tgggcgatct tgggtcggtc gatcgacttc
     1561 cagtacaggt cgttgatcgc gtcggagagc tccaccacga cgtcgggcac ctcggcccag
     1621 tcgatcgtga gctgggtgtc ggtccagtgc agcacgtggt gctggtgcag ccaggcgaac
     1681 agcagctggc cgccgagccc gtcgtagttg cgcacacgtg aaccggtgat cgcgaaccgg
     1741 aagatgcggt cgaagatcac ggcgtactgc acgagcttgg cgtgcgaccg cgtgttctcg
     1801 tcggtctcgg gcgcgcgctc caaccgcacc gcctcacgga acgccgtgag gtcgcaccgc
     1861 agctcctcca gcgagtacag gaagtacggc atgcgctgct tgatcatgaa cgggtcgaac
     1921 ggcaggtcgc cgcgcatgtg ggtgcggtcg tggatgaggt cccacatcac gaaggtctcc
     1981 tccgtgagcg cctggtcgtc gagcagagcc gcggcatcct cgggcaggtc gagcttcgtg
     2041 atctcggatg ccgcgcgcac cacccgacgg aaccgtgcgg cctcgcggtc ggcgaagatc
     2101 gccccccacg tgaagtgcgg gatctcgcgc atcgccaccg tctcggggaa cagcacggcc
     2161 gagttggtgt cgtagccctc ggtgaagtcc aggaaccgga tcggcacgaa cagcgcgttc
     2221 gagtactcgc ccgcctcgag gtcggcgatg aactcgggcc agatcacctc gatgagcacc
     2281 gcctcgacgc gacggtcact ggagccgttc tgggtgtaca tcgggaagac gaccaggtgg
     2341 cgcatcccgt cgacgcggtc gcgctgcggc tggaaggcga cgagcgagtc gaggaagtcg
     2401 ggctcgccga acccgccctc ggcccagcgg gcgaagtcgg cgtcgagcgc ggtgaggtaa
     2461 tcggcatcgt gcgggaagtg cccggccagc tcgcggatat cggcgcggat ggtcgcgatc
     2521 agggcccggc agtcgccgtg catggtggca tcggggatgc tgccgtcctt gacctggtgc
     2581 gtgcgcagcg agctgacagc cgacttgagg cgacgccagg cggggaggcc gacgatgtcc
     2641 tcgacgacct cgggctctcc tatgatcgcg tcgcgtgtgg gggtaacagt gactcgggac
     2701 atggttcacc tccgatccga gattcaggaa acgtgcatca tttccagcgt attcgcacgg
     2761 tcgtggcgtt tggttgcgat tgacgagaga aacgtgcgtg aaacgcggtc tcgacgccgc
     2821 atccggcgtg atgccccaga cttgacccat gctgggtgtg ctcgtggggt tcgcgatcat
     2881 cgccgcggtc atcctcgtgg gctacatcgt gggccggtcg ggcgtgctcg gcgagcgcgg
     2941 tggattcgtg ttgggccgac tcgccttcta cgtgctcgcg ccctgcctgc tgttcaccac
     3001 gctcgccgat gccgacgtgc acgcgctgtt ctcgtcgctg ctcttcgtgt cgctcaccgc
     3061 ggccgtcgtg tcagcaggag tgttcgtcgt gatcgcgcgg gccttctgga agcgccccgt
     3121 ctcggagacc gtcgtcggcg ccctcggctc cggctacgtc aacgccaaca acatcggcat
     3181 cccggtcgcc gtctacgtgc tgggggatgc cgcgacctcc gcccccgtga tcctcctgca
     3241 actgctgctc ttcgcgccca tcgcgctcac gatcctcgac gtgcaggctc gcggccacgc
     3301 ctcgatccgg cgcatcctgc tcgggccggt gaccaacccg atcatcctcg gatcgctgct
     3361 gggcgtgctg ctgtcgatct tccaggtgcg cttgcccgac gccgtcatgg agcctttcca
     3421 cctgatcggt gcggctgcgg ttcccgtcgt gctcatcagc ttcgggatgt cgctgcacgg
     3481 cacccgcccg ctggcaccgg ggtcatcccg ccgcgacgtg attctcgcca gcaccctcaa
     3541 gctcaccctc atgccgctcg tggcgtggct ggtcggcagc ttcgtgttcg gcctcagcgg
     3601 gcaccgcctg ttcgcggtgg tggtgctcgc cgcgctgccc gccgcccaga acgtcttcaa
     3661 ctacgcccag cgctacgacc gcggcgtgat cctcgcccga gacgtcgtgc tcatcacgac
     3721 agccctctca ctgccggtgc tgctggtgat cgcggcgctg ctcgcccctg cgggctgagc
     3781 ggcgcgcggc gcggctggac acccgggccg ccccagactc gcacttcagg agaaatgcac
     3841 gacacgccgc ctaagggatg cccgcggcac ggcgtgtcgc acagatctcc tgaacggcga
     3901 acgcgggagg tcaacggagc cgcgggccga gcgccgggcg gcagcgctag aggaacatcg
     3961 gccgatcgtc gtcgtcctcg atgtcgagcg cgaggtcgac caccacgggc acgtggtcgc
     4021 tgggggcgtc gcccttgcgc tcttcgcgga tgatgcgggc atccgtcacc agggcatcga
     4081 acgctcgcga gccgaggatg aagtcgatgc gcatgccctc gttgcggggg aagcgcagct
     4141 gctggtagtc ccagtaggtg tagccctcga cgccgcggga gcgcagggca tccgtgaggc
     4201 cggcggcctc gagtgctgcg aaggcgtcgc gctcgggctg gctgacgtgg gtcttgccgg
     4261 caaaggcggc gatgtcccac acgtcgtgat cgaagggcgc gatgttgaag tcgcccatga
     4321 gcgcgagggg aaggtcgggc tcggcggcga gccaggcgcg cgtctcggtc gcgaggttgg
     4381 cgagccactc gagcttgtac gagtagtgcg ggttgtcgag ctcacggccg ttgggcacgt
     4441 agaggctcca cagccgcacg ccctcgacgg tcgcaccgat cgctcgggcc tcctgcggca
     4501 gcgtgccgtc gtcgaggggc ttgccgaagc cgggggccga cgggaagccg atggagacat
     4561 ccgtcagggg aaggcggctc gcgatggcca caccgttcca ctggctgagg ccgtgcagct
     4621 cgacctcgta gcccgcctcg gagaacgcct cgaacgggaa ctgcgcctcg gtgcacttga
     4681 tctcctgcat ggcgagcacg tcgacgtctt cacgcatcgc ccagtcgacg acccggccga
     4741 cccggcttct gatcgagttc acattccagg tggcgacgcg catgactcca cgctacaagc
     4801 ttcaaccgac gctcgaacca cgatcggttc gcccccgtac tgggacgcga caacgagaca
     4861 tcctcagccc tagagtgcga gtgtggtgga ccatgacgag cttcttgacg cggatgccgc
     4921 ggcggccgat cgccgcgacc tgcgacgcat cgtagtcatc accaccatct cgctcgcgct
     4981 cgtcgtgctg gccagcctgc tgctggtcgc cagtaccatc cacgtctgaa tccggcctcg
     5041 gatcgagtcg ctaggctggt cgggtgaccg acgccaggca gcagctcatc gatttcatcg
     5101 cggcagaagc ggtgttccac ggtgacttca cgctcaccag cggcaagaag gcgacctact
     5161 acgtcgacct gcgcaaggtc agcctcgacc accgcgtcgc tcccctcatc ggccaggtca
     5221 tgatcgacct catcgccgat gtgcccgatg tcttcgccgt gggcggcctc accatgggtg
     5281 ccgacccgat cgctgctgcc gtgctgcacc agggcgccgc gcgcggcctc gcctacgacg
     5341 cattcgtcgt gcgcaaggag ccgaaggacc acggtcgcgg ccgtcaggtc gagggcccgg
     5401 atctcgcggg caagcgcgtc atcgtgctcg aagacacctc gaccaccggg ggttcgccgc
     5461 tcgccgccat cgaggccctg aagaaggtcg gcgccgagat cgcgggagtc gccgtggtcg
     5521 tcgaccgcaa caccggcgcg cgggagcgca tcgaagaggc cggttacccc tacttcgcgg
     5581 cgatcggatt ggccgacctg gggcttcccg gatgacggac gaccgtactc cggacgacga
     5641 gctcgacccc agcgactggc tggcgagtca gttcggcgct cccgagcccg aggtcgcccc
     5701 gcccgcggat gcccgcccgc ccgtgacacc gccgcagcct gtcgtgccgc tgaccgccgc
     5761 gccgccgact cccgccgcac ccccagcggc gcccgctccc gccgtgccgc cgttcgccgc
     5821 ccc
//
LOCUS       DAOSOU010000131         5815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13911_length_5815_cov_6.028299, whole genome shotgun sequence.
ACCESSION   DAOSOU010000131 DAOSOU010000000
VERSION     DAOSOU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13911_length_5815_cov_6.028299"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLW15_07865"
     CDS             <1..667
                     /locus_tag="PLW15_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOT34345.1"
                     /translation="VVVDEAQELSPMQWRLLMRRNPLKSFTIVGDVGHASAAAGTTSW
                     EGALRPHVGDNWRLEELTVNYRTPAQISEVAESMAIAHGVTITRSRAVRASEWPVEIV
                     RATQLAAAVADAVAAEREADAPGTVAVIASESLLPGIHPTLVERFGSHVGRGAAGLSR
                     AIAVLSPQEAKGLEFDAVIVVEPQAIVDEIPRGAAALYVAMTRPTQRLTLVTSGPLPA
                     GIE"
     gene            906..1706
                     /locus_tag="PLW15_07870"
     CDS             906..1706
                     /locus_tag="PLW15_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOT34346.1"
                     /translation="MAQGRRLRERVAGQTAMSDVVHSPSVNRERGTLARIAGVSPLDS
                     TSRRAYRIALGELVVGDILDGLGQRWDVLHDLPLGGDSTLEHLVMGPSGIFAVRTANH
                     GDVEVVVDGDIVLAGGEPHDDIEVCVRQAVAAAQALTTAAGETVLVQPLLVVVAPRRL
                     TVRREPSGVLISESSGLERMLTRAPSQLGGEQVARISDLADLETTWPEASEVTLDTQR
                     LNRQFTLVRAEVRSALAIRTAWVVGALLVACTGLWMMIARFVTQMVAA"
     gene            1751..2203
                     /locus_tag="PLW15_07875"
     CDS             1751..2203
                     /locus_tag="PLW15_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOT34347.1"
                     /translation="MFLRHPILTVLTFAYLALVGWVTLSPQSTIEQGSLLWRLAALLD
                     RIPGADWFTFSRLEFLANVAMFVPLGLFFVLLLGRSRWWLSILLGVVMTVGIELAQQS
                     IAGRVSDPRDVVANSLGAVIGTLLALVLTAGKARRIRREARLSTPSAV"
     gene            2239..2898
                     /locus_tag="PLW15_07880"
     CDS             2239..2898
                     /locus_tag="PLW15_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOT34348.1"
                     /translation="MRRLWAFVPFLVVGVIHLAALFLGVEPLSSPTKYFLMPALLVGF
                     LIALPRRRSELALVGSAAIVLSWLGDVLLSAPGDLGFLIGLGFFLLAHLAYIAQFTRS
                     LRVRRVPLIALVYVPWWVVLLIALTPYAGALVVPVAIYGAVLAVSAATALGANRIVAV
                     GALVFFVSDTLLGFKMFWPGFSLWQADFLIMLAYITGQGLIALGSVRHAQRSVGDAVP
                     A"
     gene            complement(2872..4053)
                     /locus_tag="PLW15_07885"
     CDS             complement(2872..4053)
                     /locus_tag="PLW15_07885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HOT34349.1"
                     /translation="MTDAIVLDVLLIVLLIAYVVYGFRNGLSRSIFVIAGVVAGAIAA
                     FFVVPLVTGWVAWSLARPVVTIAVTVALVAAGHALGSSLGRGIRRGIERTPLSGLDRL
                     LGAVVTGIAAALVASVVGSTVSQLGVPVLSRATAGSAVLRTINALTPDPVEAWVAQLK
                     SIVADSGLPVISDALGGPAPVIPQLDTGSPQLNAAAQSVVRITGNAFACGVSQSGTGF
                     VVADDRVVTNAHVVAGVTEPVVEAPNGEAIAGSIVYFDPVLDIAVIAVPGLTADPLPL
                     GDSLPAGTDAAVQGYPFGGPFTSGAARVMKVGTAQVDDIYGTSTHPREVYTLAADVRQ
                     GNSGGPVLTLDGTVAGVVFARSADTASVGFAMTMAELDPVAAEASTLTATVSSGDCIS
                     D"
     gene            4502..5281
                     /locus_tag="PLW15_07890"
     CDS             4502..5281
                     /locus_tag="PLW15_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOT34350.1"
                     /translation="MVRQTAPTVRVRGAAYGVIAESLRVQADARPQTLAAKVFGVSPL
                     SADARSWYRAALGQIEIVRSLNSLGSHWTLLHPVAGSSDSDYLVIGPGGVFVITIKNH
                     SGQRVVVESEQLLVNNRRTNHLRDARFEAARVSKLLSTEASESIAVNPLIAIVDPGSL
                     AFVRRRPQDVTVAASSQLARVVLRRRKILSVAAARALVAAAERTGAWHSSEHVIDDTL
                     RHEARFQRLRYEVDAAARRSVAWIVLAAAAVLAIVLAVYFL"
     gene            complement(5308..5757)
                     /locus_tag="PLW15_07895"
     CDS             complement(5308..5757)
                     /locus_tag="PLW15_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HOT34351.1"
                     /translation="MTFFESIKTVFRKYATFEGRATRPEFWWFALFSFLVSAALSALL
                     PGQYMMGGYQPINNDVVMLGDAFRSGPVINLAGFWSIAVLLPSLAVTVRRLRDAGHRW
                     TEIFWILLPIAGAIVLIVRLCDPSKSDAVVAPAAPAAPPAPPAKPAK"
BASE COUNT          862 a         1940 c         2018 g          995 t
ORIGIN      
        1 cgtcgtggtc gacgaggcgc aggaactctc cccgatgcag tggcggctgc tcatgcggcg
       61 aaacccgctc aagtcgttca ccatcgtggg cgatgtcggc cacgcgagcg cggctgccgg
      121 caccaccagc tgggagggtg ccctgcgccc ccacgtcggc gacaactggc gtctcgagga
      181 gctgaccgtc aactaccgca cccccgccca gatcagcgag gtcgccgagt cgatggcgat
      241 cgcgcacggc gtgaccatca cccggtctcg cgcggtacgc gcgagcgagt ggccggtcga
      301 gatcgtgcgc gcgacccagc tcgctgccgc cgtcgccgat gccgtcgccg ccgagcgtga
      361 ggcggatgcc cccggcacgg tcgcggtgat cgccagcgag agcctgctgc cgggcatcca
      421 ccccactctc gtcgagcggt tcggatcgca cgtgggacgc ggcgcggccg ggctgtcgag
      481 ggcgatcgcg gtgctgagcc cgcaggaggc caaggggctc gagttcgacg ccgtgatcgt
      541 ggtcgaaccg caggcgattg tcgacgagat tccgcgcggg gcggcggcgt tgtacgtcgc
      601 gatgacacgt ccgacccagc gcctcacgct cgtcacatcg ggcccgcttc cggcgggaat
      661 cgagtaacac cagtcacagg cgccccggca gccacacgtc ccccatttcg gggatgcccc
      721 caagtggggg tttgcagcac attcacagga tggccacact gtaactacga gcccgctctt
      781 cgggggaaga aggttcgatc gggtgcgatt cgtcgcagcg agtggggccg gaggcggggg
      841 agcctccggc ccaacgtcgt cttacgggcc gtgtgagttc cccgacacga tcaggggaga
      901 ccaggatggc gcagggtcgc agactccgtg agcgggtcgc cggccagacc gcgatgagcg
      961 acgtcgtgca ctcgccatcg gtgaatcgcg agcgcggcac gcttgcccgc atcgccgggg
     1021 tcagtccgct agattcgacg agtcgacgcg cctaccgaat cgccctcggc gaactcgtgg
     1081 tgggcgacat cctcgacggc ctcggccagc ggtgggacgt gctgcacgac ctgccgctcg
     1141 ggggcgacag caccctcgaa catctggtga tgggtccctc cgggatcttc gccgttcgaa
     1201 cggccaacca cggagacgtc gaggtcgtcg tcgatggcga catcgtgctc gccggcggtg
     1261 agcctcacga cgacatcgag gtctgcgttc gtcaggccgt tgcggccgcc caggcgctga
     1321 caactgccgc gggcgagacc gtgctggtgc agcccctgct tgtggtcgtg gctccgcggc
     1381 gtctgaccgt gcgacgggag cccagcggcg tactgatcag cgagtcatcc gggctcgagc
     1441 gcatgctcac ccgcgccccg agccagctcg gcggagaaca ggtcgctcgc atctccgatc
     1501 tcgccgatct ggagacgacc tggcccgagg catccgaggt cactctcgac acccagcgac
     1561 tgaatcggca gttcacgctg gttcgtgcgg aggtgcgctc cgccttggcc attcgcaccg
     1621 cctgggtggt cggcgcgctt ctggtggcgt gcaccggact ctggatgatg atcgcccggt
     1681 tcgtcaccca gatggtcgcc gcataggcgg tgtgcagggt tcgcacgagt tcatcgggca
     1741 cgatgggtga atgttcctga ggcacccgat cctgaccgtg ctgacgttcg cctacctggc
     1801 gctcgtcggg tgggtcacgc tgtcgcccca gtcgacgatc gaacaggggt cgctgctctg
     1861 gcgcctcgcc gccctgctcg accggatacc gggggccgac tggttcacgt tctcgcgtct
     1921 cgagtttctc gccaacgtgg ccatgttcgt gccgctgggg ctgttcttcg tgctcctgct
     1981 cgggcgctca cgatggtggc tgtcgatcct cctcggggtg gtgatgacgg tggggatcga
     2041 actcgcccag cagtcgatcg cgggccgcgt ctccgatccg cgggatgtgg tggccaactc
     2101 cttgggtgcg gtgatcggaa cgctgcttgc gctggtgctg acggccggca aggcgcgtcg
     2161 gattcgccgt gaggcccgac tttccacacc gagcgcggtc tgatgggcag cgagtctgag
     2221 tccgcgtacg ctgagcgagt gagacgactc tgggctttcg ttcccttcct ggtggtcggg
     2281 gtcatccacc tcgcggccct gttcctcggc gtggagccgc tgtcgagccc gacgaagtac
     2341 ttcctgatgc ccgcgcttct ggtcggattc ctgatcgcgc tgccccggcg gcgctccgag
     2401 ctcgctctgg tcggttcggc cgcgatcgtg ctgtcgtggc ttggcgacgt gcttctctcg
     2461 gcgccgggcg atctcggatt cctgatcggg ctcggcttct tcctgctcgc ccacctcgct
     2521 tacattgctc agttcacccg ttcgctgcgc gtgcgtcggg tgccgctgat cgcactggtg
     2581 tacgtgccgt ggtgggtcgt gctgctgatt gccctcacgc cctacgcggg agcactcgtg
     2641 gtcccggttg ccatctacgg tgcggtgttg gcagtgagtg cggcgaccgc gctgggcgcc
     2701 aaccgaattg tcgcagtcgg ggcgctggtg ttcttcgttt ccgacacact tctgggcttc
     2761 aagatgttct ggccgggctt cagcctgtgg caggcggact tcctcatcat gctcgcgtac
     2821 atcacggggc agggcctgat cgcgctggga tcggtgcgcc acgcgcagcg gtcagtcgga
     2881 gatgcagtcc ccgcttgaga ccgtcgcggt gagggtggat gcttcggccg ccaccgggtc
     2941 gagttcggcc atcgtcatcg caaacccgac actcgcggta tcggcgctgc gggcgaacac
     3001 cactccggcc acggtgccgt cgagtgtgag caccggtccg cccgagttgc cctgccgcac
     3061 gtccgcggcc agcgtgtaca cctcgcgcgg gtgggtgctg gtgccgtaga tgtcgtcgac
     3121 ctgtgccgtg cccactttca tgacccgcgc ggcaccggac gtgaacgggc caccgaaggg
     3181 gtaaccctgc accgcggcat ccgtgccggc gggcagtgag tcgccgagcg gcaaggggtc
     3241 tgccgtgagc ccgggaaccg cgatgaccgc aatgtcgagc acggggtcga agtagacgat
     3301 cgagccggcg atcgcttcac cattcggtgc ttcgacgacc ggctcggtga cgccggccac
     3361 cacatgagca ttcgtgacga cgcgatcgtc ggcgaccacg aagccggttc ccgactgcga
     3421 gactccgcac gcgaaggcgt tgccggtgat gcgcacaaca gattgcgcgg ccgcattcag
     3481 ctgggggctg ccggtatcca gctggggtat gacgggtgcg ggcccaccga gggcatcgga
     3541 gatgacgggc aggccgctgt cggccacgat cgacttgagc tgggcgaccc aggcctcgac
     3601 cgggtcaggg gtcagcgcgt tgatggtgcg tagcactgcc gaccccgccg tcgctctcga
     3661 gagcaccggc acaccgagtt ggctcacggt cgacccgacc accgacgcca cgagcgctgc
     3721 ggcgatcccc gtcaccacgg cgccgagcag cctgtccagc ccgctcagcg gggtgcgctc
     3781 gatgccgcga cggatgccac ggccgaggct cgagccgagt gcatgccctg ccgccaccag
     3841 ggcaactgtg acggcgatcg tcaccacggg gcgggccagt gaccacgcga cccagcccgt
     3901 caccaggggc accacgaaga acgcggcgat cgcgccggcg acaacgccgg caatgacgaa
     3961 gatgctccgg gacaggccat tcctgaagcc gtagacgacg tatgcgatga gaagcacgat
     4021 gaggagcacg tcgagcacga tcgcgtcagt catggctgcg agcctacgcg gcgcaactcc
     4081 cagaagaccg cgaagggacc tttgacccta tgcgccgggt gcggtgcaga ggagagtgag
     4141 tttcacgaag gcggtcgggg gctgaccgtg gagcgcgtga ttcgggttca ggcgctcatc
     4201 ttcttacggg gtacatcccg gatgccgcag acgggggtcg cggcatccgg gcactctcat
     4261 cgccaccggc accatcaggg tgcgggtcgt cgtcatccac gggatctggc gtctcgttgc
     4321 caccatgttc ctcgaggcga ggttgccgac gcgctgacgc tcgacgcgcg cgccccgtag
     4381 tggggactgc cgcggcatcc gggtgactgg ctagcgtgga gtcatcgaca ttcgggggga
     4441 tgtggtgaca gatcttttgg ctcgcccact gggcgaggag cgtgagggta ggggccctga
     4501 cgtggtgcgc cagaccgcgc cgaccgtacg tgtgcggggt gcggcctacg gcgtcatcgc
     4561 ggagagcctc cgcgtgcagg ccgacgctcg cccgcagact ctggcggcaa aggtcttcgg
     4621 cgtcagcccg ctcagtgccg atgcgcgcag ctggtaccgc gccgccctcg gccagatcga
     4681 gatcgtccgt tccctgaact ctctcggctc gcactggacg ctcctgcatc cggtggcggg
     4741 cagctccgat tccgactacc tcgtgatcgg ccccggtggc gttttcgtca tcaccatcaa
     4801 gaaccattcc ggtcagcgtg ttgtggtgga gagcgagcag ctgctcgtca acaaccgccg
     4861 caccaaccac ctccgggatg cccgcttcga ggccgcacgg gtctcgaagc tcctgagcac
     4921 ggaggcgagc gagtccatcg cggtgaaccc cctcatcgcg attgtggacc caggctcgct
     4981 cgccttcgtt cgacggcggc cacaggacgt cactgtggcc gcttcttcac aactggcgcg
     5041 tgttgtgctg cggcggcgga agatactctc cgtcgccgca gcccgcgctt tggtggcggc
     5101 cgccgaacgc accggcgcgt ggcacagctc cgagcacgtc atcgacgaca ccttgcgtca
     5161 cgaggcgcgc ttccagcggc ttcgctacga ggtggatgcc gcggcgcgcc gcagcgtcgc
     5221 ctggatcgtg ctcgcggccg ccgccgtgct cgccatcgtt ctggcggtgt acttcctgta
     5281 gttcgcgcta cttgccgcta cttgccgcta cttggccggc ttcgcgggcg gagccggggg
     5341 agcggccgga gcggcgggag cgactaccgc gtcactcttc gacgggtcgc acaggcgcac
     5401 gatgagcacg atcgcgccgg cgatcggcag caggatccag aagatctccg tccaccggtg
     5461 tccggcatca cgcaggcgac gcacggtgac ggcgaggctc ggcagtagca cggcgatgct
     5521 ccagaagccg gcgaggttga ttaccggtcc tgagcggaac gcgtcgccca gcatgacaac
     5581 gtcgttgttg atgggctggt agccgcccat catgtactgg ccaggcagca gtgcactcag
     5641 cgccgcgctc accaggaagc tgaacagcgc gaaccaccag aactcggggc gggtggcccg
     5701 gccctcgaac gtggcgtact tgcggaacac ggtcttgatg gactcgaaaa aggtcatgca
     5761 gcgagcttgt cacgttccgg tgccgcccgg gagggggcta acccccgatt cgcgc
//
LOCUS       DAOSOU010000132         5794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_13979_length_5794_cov_5.109252, whole genome shotgun sequence.
ACCESSION   DAOSOU010000132 DAOSOU010000000
VERSION     DAOSOU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13979_length_5794_cov_5.109252"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="rsmA"
                     /locus_tag="PLW15_07900"
     CDS             complement(<1..817)
                     /gene="rsmA"
                     /locus_tag="PLW15_07900"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT34352.1"
                     /translation="MRDPLVSGLLGPAEIRDLAELLGIQPTKKLGQNFVIDANTVRRI
                     VKSAGVTRGETVVEVGPGLGSLTLGLLEAGASVVAVEIDKRLAAQLPLTVAELREHQG
                     PDEAPTLTVITEDALRVTELPGEPTVLVANLPYNVSVPVLLHFLEHFPSIQRGLVMVQ
                     AEVGERVAAGPGSKVYGSPSIKAAWYGTFTTAGKVSRQVFWPVPNVDSILVRFERHEQ
                     PGTEAERLATFELVDAAFQQRRKMLRQALAVTLGDSAAASARLEAAGIAPTARG"
     gene            complement(852..1709)
                     /locus_tag="PLW15_07905"
     CDS             complement(852..1709)
                     /locus_tag="PLW15_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT34353.1"
                     /translation="MRQRESGSRDLTYPPLPEPLAVAVYDNHTHLEISDGEPGGELDY
                     LEQLDRAERVGVKGVVQVGGDLETSRWSASVAATEPRMLAAVALHPNEAPGFADLDAA
                     LAEIDALAAQPRVRAVGETGLDFFRTGEEGRDAQYRSFEAHIEIAKKHGIALQIHDRD
                     AHAEVIATLKRVGAPEQTVFHCFSGDAEMARIAADHGWYLSFAGTVTFKNAAGLREAL
                     SVTPLDRIMVETDAPYLTPSPFRGRPNSPYLIPLTLRAMAEHLDVDVDELAAQVASTT
                     EHVYGSWAL"
     gene            complement(1784..3340)
                     /gene="metG"
                     /locus_tag="PLW15_07910"
     CDS             complement(1784..3340)
                     /gene="metG"
                     /locus_tag="PLW15_07910"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOT34354.1"
                     /translation="MAAGDSFYITTPIFYVNDVPHIGHAYTEVAADVLARWHRQRGDD
                     TWLLTGTDEHGQKILRTAVANDTTPQAWADKLVEDAWKPLLQTIDISNDDFIRTTEPR
                     HEKAVALFLQRLYDDGHIYSGEYEGYYCVGCEEYKQIDDLMETPDGDYAGQLVCKIHG
                     RPVEILKEKNYFFRMSAFEDRLLDLYENTQDFVQPSSVRNEIIQFVKQGLDDLSISRS
                     SFDWGIKVPWDDSHVVYVWFDALLNYITAVGYGTDDEQFERRWPAVHLVGKDIARFHA
                     VIWPAMLMAAGLEVPRAVFGHGWLLVGGEKMSKSKLTGIAPSQITDTFGSDAFRYYFL
                     SAITFGQDGSFSWEDLSARYQAELANGFGNLASRVIAMVIKYFDGSIPAAPLDEVITS
                     IEKRVTDAANTQIDAFAINEALATIWELVDALNGYITDTEPWALAKDPAQRARLESVL
                     ATAVHGLGTLAVLLAPVLPKATARLWEAIGGSGTVESQRIDRAWEFDGGSRVTALEAS
                     LFPRIEAEAD"
     gene            3405..4079
                     /locus_tag="PLW15_07915"
     CDS             3405..4079
                     /locus_tag="PLW15_07915"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HOT34355.1"
                     /translation="MDYTVVDVIGHALPLAVGVALSPFPLISVVLLLMSPQGRAAGAT
                     FLVGRILGLAVMLAIIIAASELLYSAANGTGIPTVIKLLIGVALIVLGLSKWRPKPAG
                     TEPKLPGWMNAIDGFSPARAFGLGLLLSITNPKELALAIPLGVTIGGALLAPGETVLV
                     GAIFVLVASISIAVPVIALAIAPTRMRPALDRLRAWLTQNHSVVMGVLLIVIGAVVAG
                     GAIADF"
     assembly_gap    4204..4303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4332..5147)
                     /gene="rsmI"
                     /locus_tag="PLW15_07920"
     CDS             complement(4332..5147)
                     /gene="rsmI"
                     /locus_tag="PLW15_07920"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT34356.1"
                     /translation="MIILAATPIGNLGDASRRLIEALSTAEVVASEDTRTTIHLMRAL
                     GIENRPRLIPLHEHNETTVAAELVELARSTDVLVLSDAGMPAISDPGFPLVAAAAAAG
                     VTVTALPGPSAVLTALAVSGLPTDRFTFEGFLPRKGRVAYFAGLAREPRTMIFFESPN
                     RLGAALADLATALGADRRVVVCRELTKLHEEVVRGSAADVAQHFADGARGEIVVVVDG
                     AAAVASDLPTGIAQVLALVADGAKLKEASAEVADATGLSRRDLYEGALAARKA"
     gene            5320..>5794
                     /locus_tag="PLW15_07925"
     CDS             5320..>5794
                     /locus_tag="PLW15_07925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT34357.1"
                     /translation="MEGEQVEQRTGSRLDDWWQSRSPRLRRILRWAAPAMVVVIAAVT
                     RLINLGSPHTLVFDETFYVKDAWTLVNLGYEGQWPAEADALFAGGQTDIFLADPSFVV
                     HPPLGKWLIGLGMLIFGPENSFGWRVATAVTGILAVVLIMLIARLRKVPQGILFNA"
BASE COUNT          862 a         1932 c         1946 g          954 t
ORIGIN      
        1 cgccgcgggc ggtcggcgcg atgcccgccg cctcgagccg ggccgacgcg gcggccgagt
       61 cgccgagggt gacggcgagc gcctgacgca gcatcttgcg gcgctgctgg aacgcggcat
      121 ccacgagctc gaaggtcgcg aggcgctcgg cctcggtgcc cggctgctcg tggcgctcga
      181 aacgcacgag aatcgagtcg acattgggca ccggccagaa cacctgtcgg ctcaccttgc
      241 ctgccgtggt gaacgtgccg taccaggccg ccttgatcga tggtgagccg tagaccttcg
      301 agccggggcc agcggcgacg cgttcgccga cctcggcctg caccatcacg agcccgcgtt
      361 ggatcgacgg gaagtgctcg aggaagtgca gcagcaccgg cactgacacg ttgtacggca
      421 ggttggcgac gagcactgtc ggctcgcccg gcagctcggt gacgcgcagg gcgtcctcgg
      481 tgatcacggt gagtgtcggc gcttcgtccg gtccctgatg ctctcgaagc tcggcgacgg
      541 tcagcggcaa ctgcgcggcc agtcgtttgt cgatctccac cgcgacgacg gatgccccgg
      601 cctccagcaa cccgagcgtc agcgaaccga gtcccgggcc aacctccacg accgtctcgc
      661 cgcgcgtcac cccggccgac ttcacgatgc ggcgcaccgt gttggcatcg atgacgaagt
      721 tctggccgag cttcttggtg ggctggatgc cgaggagttc agcgaggtct cggatctcgg
      781 ccggacccaa caggccgctc acaagcgggt ctcgcatctc gacaggctcg atgaccgagg
      841 cacgacccca ctcatagcgc ccacgacccg tagacgtgct cggtggtgga ggcgacctgc
      901 gctgccagct catccacgtc gacgtcgagg tgctcggcca tggcgcgcag ggtgagcggg
      961 atgaggtacg gggagttggg acgacctcgg aacgggctcg gcgtgaggta cggggcatcc
     1021 gtctcgacca tgatgcgatc gagaggcgtc accgacaggg cttcgcgcag ccctgcggcg
     1081 ttcttgaagg tgacggtgcc ggcgaatgac aggtaccagc cgtggtcggc cgcgatgcgg
     1141 gccatctcgg cgtcgcccga gaagcaatgg aacaccgtct gctcaggggc acccacacgc
     1201 ttgagggtcg cgatgacctc ggcatgggca tcccggtcgt ggatctgcag cgcaatgccg
     1261 tgcttcttgg cgatctcgat gtgcgcctcg aacgagcggt actgggcgtc gcgcccctcc
     1321 tcgccggtgc ggaagaagtc cagaccggtc tcccccacgg cccgcacgcg cggctgggcc
     1381 gcgagcgcat cgatctcggc gagagcggca tccaggtcgg cgaaccccgg cgcctcgttg
     1441 ggatgcagcg ccacggcggc aagcatccgc ggctcggtcg cagccaccga tgccgaccag
     1501 cgcgacgtct ccaggtcgcc gccgacctgc acgacgccct tcactcccac tcgctcggca
     1561 cggtcgagct gctcgaggta gtcgagctcg ccgccgggtt caccatcgga gatctcgagg
     1621 tgggtgtggt tgtcgtagac cgcgacggcc aacggctcag gcaggggcgg gtaggtcagg
     1681 tcgcgcgagc cggactctcg ctgccgcacg aacgcgggtt gagtaggcga agccgtatcg
     1741 aacccacctc caccgtgcga tttcgataca cgctccgcgt tactcaatct gcctcggctt
     1801 cgatccgcgg gaacagggat gcctccagcg ccgtcacgcg cgagcctccg tcgaactccc
     1861 aggcccgatc gatgcgctgc gactcgacgg taccggaccc gccgatcgcc tcccacaggc
     1921 gcgcagtggc cttcggcagc accggcgcga ggagcacggc gagagttccg agaccgtgca
     1981 cggccgtggc cagcaccgac tcgagccgcg cgcgctgggc cgggtctttg gcgagggccc
     2041 agggctcggt gtcggtgatg tagccgttga gggcgtcgac gagctcccag atggtggcga
     2101 gcgcctcgtt gatcgcgaag gcgtcgatct gggtgttcgc ggcatccgtc acccgcttct
     2161 cgatcgacgt gatgacctca tcgagcggcg ccgccgggat cgacccgtcg aagtacttga
     2221 tcaccatggc gatcacgcgc gaggcgaggt ttccgaaacc gttggcaagc tcggcctggt
     2281 agcgggcgct gagatcctcc cagctgaacg agccgtcctg gccgaaggtg atggccgaga
     2341 ggaagtagta gcggaatgca tccgaaccga aggtgtcggt gatctgcgag ggcgcgatac
     2401 cggtgagctt ggacttcgac atcttctcgc cgccgacgag cagccagccg tgaccgaata
     2461 ccgcgcgcgg cacctcgagg cccgcagcca tgagcatggc aggccagatc accgcgtgga
     2521 agcgggcgat gtccttgccc acgaggtgca cggctggcca gcggcgctcg aactgctcgt
     2581 cgtcggtgcc gtagccgacc gcggtgatgt agttgagcag cgcgtcgaac cagacgtaga
     2641 cgacgtgact gtcatcccac ggcaccttga tgccccagtc gaagctcgat cgcgagatgg
     2701 acaggtcgtc gaggccctgc ttgacgaact ggatgatctc gttgcgcacg ctcgagggct
     2761 gcacgaagtc ctgggtgttc tcatagaggt cgagcaggcg atcctcgaac gcactcattc
     2821 ggaagaagta gttcttctcc ttgagaattt cgaccggccg accgtggatc ttgcagacga
     2881 gctggccggc gtagtcaccg tccggcgtct ccatgaggtc gtcgatctgc ttgtactcct
     2941 cgcagccgac gcagtaatag ccctcgtact cgcccgaata gatgtgaccg tcgtcgtaga
     3001 ggcgctgcag gaacagcgcc acagccttct cgtgccgggg ctcagtggtg cggatgaagt
     3061 cgtcgttgct gatgtcgatc gtctgaagaa gcggcttcca ggcatcctcg acgagcttgt
     3121 cggcccacgc ctgaggtgtg gtgtcgttgg cgaccgcggt gcgcaggatc ttctgtccgt
     3181 gctcgtcggt gcccgtgagc agccaggtgt cgtcgccgcg ctgacggtgc cagcgggcga
     3241 gcacgtccgc cgccacctcc gtgtacgcgt ggccgatgtg cggaacatcg ttgacgtaga
     3301 agatgggcgt cgtgatgtag aaggaatcgc cggcggccat gctccaagtc tacggatgcc
     3361 gcgaccagtg ttaccgcgcc gccgcggatc gctagggttg ccgcatggac tacaccgtgg
     3421 ttgacgtcat cggccatgca ctcccgctgg cggtgggtgt cgcgctcagc cccttcccgc
     3481 tgatctcagt ggtgctgctg ctcatgtcgc cgcagggacg tgccgccggt gcgacgttcc
     3541 tcgtgggccg gatcctgggt ctcgcggtca tgctcgcgat catcattgcg gcgtcggaac
     3601 tgctgtacag cgcggccaac ggcacgggca tccccaccgt gatcaagctg ctcatcggcg
     3661 tcgccctcat cgtgctgggc ctcagcaagt ggcggcccaa gcccgcgggc accgagccga
     3721 agctccccgg ctggatgaac gcgatcgacg gcttctcgcc cgcccgcgcg ttcggtctcg
     3781 gactgttgct gtcgatcacc aaccccaagg agctcgcgct cgcgatcccg ctgggcgtga
     3841 cgatcggcgg cgcactgctg gcccccggtg agacagtgct cgtcggtgcg atcttcgtgc
     3901 tcgtcgcttc gatctccatc gccgttccgg tgatcgccct cgcgatcgcg ccgacgcgga
     3961 tgcgcccggc actcgaccgc ctgcgcgcct ggctcaccca gaaccactcg gtcgtgatgg
     4021 gcgttctgct catcgtgatc ggcgcggtcg tcgccggcgg ggcgatcgcc gacttctaga
     4081 tcggtagctt ctagcgggca gcttctggcg gcagcttctg gatcggcagc ttctggcggc
     4141 tgcctctgga tcggcagctt ctggcgggcc cggctcctag gccttgcggg ccgccagcgc
     4201 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatcggc agcttctggc
     4321 gggcccggct cctaggcctt gcgggccgcc agcgcgccct cgtagaggtc acgcctgctg
     4381 agccctgtcg cgtcggccac ctccgccgac gcctccttga gcttcgcgcc gtcggccacg
     4441 agcgccagca cctgcgcaat gccggtcggc agatcggatg ccacggccgc cgccccgtcg
     4501 accacgacga cgatctcgcc ccgggcgccg tcggcgaagt gctgtgccac gtcggcggcg
     4561 gagccgcgca cgacctcctc gtgcagcttg gtcagctcgc ggcagaccac gacccgacgg
     4621 tcagcgccga gcgccgtcgc cagatcggcg agcgctgcgc cgagccggtt gggggactcg
     4681 aagaagatca tcgtgcgcgg ctcgcgggcg aggcccgcga agtaggcgac ccgacccttg
     4741 cgcggcagga agccctcgaa ggtgaaccgg tcggtgggca gcccgctcac ggcgagggcg
     4801 gtgagcacgg cgctcggtcc cggcagggct gtgaccgtga cgcctgctgc cgcggccgcc
     4861 gcgacgaggg ggaacccggg gtcggagatc gcgggcattc ccgcgtcgct cagcaccagc
     4921 acatccgtcg atctggccag ctcgaccagc tcggcggcga cggtcgtctc gttgtgctcg
     4981 tgcagcggga tcagtcgcgg ccggttctcg atgccgagcg cccgcatgag gtggatggtc
     5041 gtgcgggtgt cctcgctggc gaccacctcg gcagtgctga gcgcctcgat cagtcgccgg
     5101 gaggcgtcac ccagattgcc gatgggcgtc gccgcgagga tgatcacccc gccattctcc
     5161 caccgcccaa atgaaggaga tccttcgctg cacgagcacg ctcatgcgcg tcggcgcccc
     5221 acacactcag gaactccttc atttggtctc gcatacgatc tagggatgag ccagcccacg
     5281 accgcagagg agttcgacga cctcctcgac ggcgagccga tggagggcga gcaggtcgag
     5341 cagcgcacgg gttcgcgcct cgacgactgg tggcagtcgc gcagccctcg gctgcggcgt
     5401 atcctgcgct gggcggcccc cgcgatggtg gtcgtcatcg cggcggtgac ccggctcatc
     5461 aacctcgggt cgccgcacac cctcgtcttc gacgagacct tctacgtgaa ggatgcctgg
     5521 actctcgtca acctcggata cgagggccag tggccggccg aagccgacgc gctcttcgcc
     5581 ggcggccaga cggacatctt cctcgccgac ccctcgttcg tcgtgcaccc gcccctgggc
     5641 aagtggctca tcggcctcgg gatgctgatc ttcggccccg agaactcgtt cggctggcgg
     5701 gtggcgaccg cggtgaccgg catcctcgcg gtcgtgttga tcatgctgat cgcccgactg
     5761 cgtaaggtcc cgcaggggat cctgttcaat gccg
//
LOCUS       DAOSOU010000133         5779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14024_length_5779_cov_6.224493, whole genome shotgun sequence.
ACCESSION   DAOSOU010000133 DAOSOU010000000
VERSION     DAOSOU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14024_length_5779_cov_6.224493"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLW15_07930"
     CDS             <1..901
                     /locus_tag="PLW15_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyisobutyryl-CoA hydrolase"
                     /protein_id="HOT34358.1"
                     /translation="SGAGERGLCAGGDVTAMYRSAVAEGADAGVEAAAFWADEYALNS
                     TIAHYPKPYVALMTGVVLGGGVGVSAHGSHRVVTETTKLGMPEVTIGFVPDVGGTRLL
                     ARAPGELGTHLALTGGTITGADAIALGFADSYVPSVALPALLDDLERMPAADAIARHA
                     AAVPASDLLTQRAWVDEAYAGDDPVMIVERLESSPVAEARAAASVIRGKSPTSVAVAL
                     EALRRAAGLATLEQVLDQEYRVGLRMLAGADMREGIRAQVIDKDRTPHWRPPTLEQVD
                     HADVLHYFEPLGERELGLATRPA"
     gene            911..1825
                     /locus_tag="PLW15_07935"
     CDS             911..1825
                     /locus_tag="PLW15_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOT34359.1"
                     /translation="MPVPFTVSAEVADALAANRPVVALESTIISHGLPRPRNHEAAIE
                     FETILRDAGVTPATIAILDGVPQIGLDAEGVRRIAEEELAKASVRDIPIIAATGASAA
                     TTVAATSHLAALAGVRVFATGGLGGVHRGASDTFDESADLSTLAVTPITVVSAGVKSV
                     LDIAATLERLETLSVPVVGYGTRIFPSFWLRESAFTLDWSVDTAAEVAAIMRSQDQLG
                     HGQGIVVANPIPLEQQWDPTEHDRVLTEALALADEAGVTGKAVTPFLLLKIVELSGGR
                     SLEVNLDLARNNVRVAGEIATAWCALNA"
     gene            1818..>2119
                     /locus_tag="PLW15_07940"
     CDS             1818..>2119
                     /locus_tag="PLW15_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT34360.1"
                     /translation="MPEAGQRIVCFGDLIDDVVAVPQGPIRDDTDTEASIRILPGGSA
                     ANTAAWLGSLGADVDFVGIVGSADVARHEASLPGVRAHLRGHATEPTGTIVVVVQG"
     assembly_gap    2120..2219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2220..2537
                     /locus_tag="PLW15_07945"
     CDS             <2220..2537
                     /locus_tag="PLW15_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT34361.1"
                     /translation="ILLPSLEEGSLLTGLAAPEAIAAALAETFDVVALTMGMDGVIMA
                     FGDTVEHVVASHAEVVDPTGAGDAFAAGFLAEWVRTNDPIASAMCGVLVGARAVETIG
                     GRP"
     gene            complement(2824..4179)
                     /locus_tag="PLW15_07950"
     CDS             complement(2824..4179)
                     /locus_tag="PLW15_07950"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOT34362.1"
                     /translation="MSGTTIVWLRDDLRLADNPALTAAAERGEPVVLVYLLDEVSPGI
                     RPLGAASRWWLHHSLAALSASVGGRLVLRRGAAEQVIPALVAETDAAAVHWNRRYSAS
                     RDIDARLKVSLRESGIDAQSHQANLLHEPWTVTTGDGNPYRVFTPFWRASLELPVRSP
                     LPVSEVTYADATSDSLDDWNLLPTRPDWAGGLREAWTPGEAGAHARLDAFVTEGLPLY
                     HRRDEPAVAATSRLSPHLRFGEVSPFQLWEAAHGRDKNSAKFLSEVGWREFSWNILFH
                     SPRLATENFRPAFDGFPWGQADPAELAAWREGRTGIPLVDAGMRELWHTGYMHNRVRM
                     VVASFLVKNLLVDWRVGEQWFWDTLVDADEANNPASWQWVAGSGADAAPYFRVFNPEL
                     QAAKFDPDAEYIRRWVPEYGTPAYPDPIVDLGETRRQALEAYEAVKASAGGSGRESAG
                     D"
     gene            complement(4179..4616)
                     /locus_tag="PLW15_07955"
     CDS             complement(4179..4616)
                     /locus_tag="PLW15_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT34363.1"
                     /translation="MSDRAAAVNAWEALFRAQVTVLRALQAEFPTDEISLNEYDVLFN
                     LSRQPEHQLRIRDLNKHLLLTQPSVSRLVDRLAARGLLRKEADPGDGRGTIVCLTEAG
                     YELFRSVAVPHSTSIAKEMAALTPDELAALTALTDKLRRSIEG"
     gene            4956..>5779
                     /locus_tag="PLW15_07960"
     CDS             4956..>5779
                     /locus_tag="PLW15_07960"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT34364.1"
                     /translation="MTVDGVWPAVQGIAYGGDYTPEQWPRETWHDDVALMREAGVSMV
                     SIGIFSWALLETSEGVFDFDWLDDLIELLHDNGIAVDLGTPTASPPAWFFTQHPDARP
                     VSRDGVPMGFGARGMASPSSPEYREASVRVAGRLAERYGEHPAVVMWHVHNEYGQPVG
                     EDYSAHAVLAFRQWLEARYGTIAELNRVWGTAFWGQRYSDWHEVSVPAAALSVVNPAQ
                     RLDFARFTDHQLRQCYIAERDAIRAHAAQPITTNFMANSSWVTDLWAWGREVDIVS"
BASE COUNT          841 a         1988 c         1957 g          893 t
ORIGIN      
        1 ctccggggct ggcgaacgcg gcctgtgcgc cggcggcgac gtcaccgcga tgtaccgttc
       61 ggcggtcgcc gagggtgctg acgccggcgt cgaggccgcc gcgttctggg ccgacgaata
      121 cgcgctcaac tccacgatcg cgcactaccc caagccttac gtcgccctga tgaccggcgt
      181 cgtgctcggc ggcggggtcg gtgtctcggc acacggctcg caccgcgtcg tcaccgagac
      241 gacgaaactc gggatgcccg aggtcacgat cggcttcgtg cccgacgtcg gcggcacccg
      301 ccttctcgcc cgcgcgccgg gcgagctcgg cacgcacctc gcgctcaccg gcggcaccat
      361 tactggcgcc gacgcaatcg cgctcggctt cgccgactcg tacgtgccgt cggtagcgct
      421 acccgccctg ctcgatgacc tcgagcggat gcccgccgcc gacgcgatcg cccggcacgc
      481 ggcagcggtg cccgcctccg acctgctcac gcagcgcgcg tgggtggacg aggcgtacgc
      541 gggcgacgac cccgtgatga tcgtggagcg gctggagtcg tcgcccgtcg ccgaggctcg
      601 cgccgcggca tccgtgattc gcggcaagtc gccgacgagc gtggccgtgg cgctcgaggc
      661 cctgcgccgg gccgccgggc tcgcaaccct cgagcaggtg ctcgaccagg agtaccgggt
      721 ggggctgcgg atgctcgcgg gcgccgacat gcgcgaaggc atccgcgcgc aggtgatcga
      781 caaggaccgc accccgcact ggcgaccgcc caccctggag caggtcgacc acgccgacgt
      841 gctgcactac ttcgagccgc tcggcgagcg cgaactcggg ctggcgacgc gaccagcgta
      901 agttagacgg atgcccgttc cattcactgt ctccgccgaa gtcgccgacg cgctcgccgc
      961 caaccgaccg gtcgtcgccc tcgagtccac gatcatctcc cacggcctgc cgcggcctcg
     1021 caaccacgag gcggcgatcg agttcgagac gatcctgcgg gatgccggcg tcacccccgc
     1081 gacgatcgcc atcctcgacg gcgtgcccca gatcggtctc gatgcggagg gcgtgcgtcg
     1141 gatcgcggag gaggagctcg ccaaggcgag cgtgcgcgac atccccatca tcgcggccac
     1201 cggcgccagc gcggcgacca ccgtggcggc cacctcgcat ctggccgctc tcgccggcgt
     1261 gcgcgtcttc gcgaccggcg gtctcggcgg cgtgcatcgc ggggcgagcg acaccttcga
     1321 cgagtcggcc gacctgtcga ctctcgccgt gacaccgatc accgtggtca gcgcgggcgt
     1381 gaagtcggtg ctcgacatcg cggccacgct ggagcgcctc gagacgttga gcgttcccgt
     1441 cgtcggctac ggcacccgca tcttcccgag cttctggctg cgggagtcgg cgttcacgct
     1501 cgactggtcg gtcgacaccg ccgccgaggt ggcggcgatc atgcgctcgc aggatcagct
     1561 gggccacggg cagggcatcg tggtcgccaa cccgatcccg cttgagcagc agtgggaccc
     1621 taccgagcac gaccgcgtgc tcaccgaggc gctcgcgctt gccgacgagg ccggcgtgac
     1681 cggcaaggcg gtgacgccgt tcctgctgct caagatcgtc gagctctccg gcggccgcag
     1741 cctcgaggtc aacctcgatc tcgcccgcaa caacgtgcgg gtcgccggcg agatcgctac
     1801 cgcgtggtgt gcgctgaatg cctgaggccg gccagcgcat cgtctgcttc ggtgacctca
     1861 tcgacgatgt ggtcgcggtg ccccagggtc cgatccggga tgacaccgac accgaggcat
     1921 ccatccgcat cctgcccggc ggctcggcgg ccaacaccgc cgcgtggctg ggttcgctcg
     1981 gcgccgacgt cgacttcgtc gggatcgtgg gctcggcgga tgtcgcgcgt cacgaggctt
     2041 cgctcccagg cgtgcgagcg cacctgcgcg gccacgccac cgaaccgacc ggcaccatcg
     2101 tcgtcgtggt gcagggtcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2221 ttctgctgcc gagcctggag gagggcagcc tgctgaccgg gctcgccgcc cccgaggcga
     2281 tcgcggcggc gctcgcggag acgttcgacg tcgtcgcgct cacgatgggc atggacgggg
     2341 tcatcatggc cttcggagac accgtcgaac acgttgtcgc ctcgcacgcc gaggtcgtcg
     2401 acccgacggg tgcgggcgac gcgttcgcgg ccggcttcct tgcggagtgg gtgcgcacga
     2461 acgacccgat cgcctccgcc atgtgcggtg tgcttgtcgg cgcgcgtgcg gtggagacta
     2521 tcggcggtcg gccctaggtc ggttgggctt tcggcgggcc gcctgccggg cgtgatcagg
     2581 ctggcccgac cggcggccgc gaccggtctc gtgctgccct tcgcgcgaac tgcggtcact
     2641 tctgctgtgc acttctgtta tgcaggagat gggacgatcc gtcgactttc ctgcacaaca
     2701 gaagtcgtgc acccaggggg cgattgcgca cctttccaga gccgcaggag gtgcgagtgt
     2761 cgatgattgc gcgcgtttcc tgcataacag gagtagttgg cggatggcac cgtcagttcg
     2821 ccgtcaatcg ccagccgact cgcgacccga gccgccagca cttgccttga ccgcctcata
     2881 ggcctcgagc gcctggcgcc gggtctcgcc caggtccacg atcgggtcgg ggtaggcggg
     2941 cgtgccgtac tcgggcaccc agcgccggat gtactcggcg tcggggtcga acttggccgc
     3001 ctgcagttcg gggttgaaca cgcggaagta gggcgcggca tccgccccgc tgcctgcgac
     3061 ccactgccag ctcgccgggt tgttggcctc gtcggcgtcg acgagggtgt cccagaacca
     3121 ctgctcgccc acccgccagt cgacgagcag gttcttcacg agaaagctcg ccacgaccat
     3181 ccgcacccgg ttgtgcatgt agccggtgtg ccagagctcg cgcatgccgg catccaccag
     3241 cgggatgccc gtgcgcccct cgcgccacgc cgcaagctcg gcggggtcgg cctgccccca
     3301 gggaaagccg tcgaacgcgg gcctgaagtt ctccgtcgca agcctcggcg agtggaacag
     3361 gatattccag ctgaactcgc gccacccgac ctcgctcagg aacttcgccg agttcttgtc
     3421 gcgcccgtgc gcggcctccc acagctggaa cgggctgacc tcaccgaacc gcaggtgcgg
     3481 gctcaggcgg ctggtcgcgg cgacggccgg ttcgtcgcgc cggtggtaca gcggcagacc
     3541 ctcggtgacg aaggcgtcga gtcgggcgtg agctcccgcc tcgcccggtg tccacgcctc
     3601 gcgcagaccg ccggcccagt cggggcgggt gggcagcagg ttccagtcgt cgaggctgtc
     3661 tgacgtggca tccgcgtagg tcacttcgga cacgggcagc ggcgaacgca ccggcagctc
     3721 gaggctcgcg cgccagaacg gcgtgaacac gcggtacggg ttgccgtcgc cggtggtgac
     3781 cgtccacggc tcgtgcagca ggttcgcctg gtggctctgc gcgtcgatgc ccgactcccg
     3841 cagcgatacc ttgagccgcg catcgatgtc gcgggaggcc gagtaccggc ggttccagtg
     3901 cacggccgcg gcatccgtct cggcgaccag cgcggggatg acctgctcgg cagcaccgcg
     3961 tctcagtacg agccggccgc cgaccgaggc cgagagtgcg gccagcgagt ggtgcagcca
     4021 ccagcggctc gccgccccca gcggccggat gcccggactc acctcatcga gcaggtacac
     4081 gagcacgacg ggctcgccgc gctccgccgc cgccgtcagc gcggggttgt cggccagacg
     4141 caggtcgtcg cgcagccaga caatcgtggt gcccgacatc aaccctcgat gctgcgccgc
     4201 agcttgtcgg tgagtgcggt cagggcggcc agctcgtcgg gagtgagcgc ggccatctcc
     4261 ttcgcgatcg aggtcgagtg cggcacggcc accgagcgga acagctcgta accggcctcg
     4321 gtgaggcaca cgatggtgcc gcgcccgtca cccgggtcgg cctccttgcg cagcaggccg
     4381 cgcgccgcga gacgatcgac caagcggctc acgctcggct gggtgagcag caggtgcttg
     4441 ttcaggtcgc ggatgcgcag ctggtgttcg ggctggcggg acaggttgaa gagcacgtcg
     4501 tactcgttga gggagatctc atcggtgggg aattcggcct gaagcgccct cagtacggtg
     4561 acctgcgcgc ggaacagtgc ctcccaggcg ttgaccgctg ccgctcgatc gctcatccag
     4621 taaaactacc gccggttgag tagcgcgaag cgcgtatcga gaccaagccg ctgggccggt
     4681 tgagtagcgc gaagcgcgta tcgaaaccga gacccccgtt aaacaggatt gagggtcggt
     4741 tccagaggct aggaaccgac cctctccctt gcaccaagag tgtcctgcaa tcacattccg
     4801 catcagctgc cacagcaaac aacttctgct ctatgaatgt ataacggaac ggtaacgctg
     4861 tctagttacc taaccgttat tttttctgcg agtttccttg gagccccaaa acactcccga
     4921 gcgagcgcgc cggccaagct gcgagactgg ggttcatgac agtcgacgga gtctggccag
     4981 cggtgcaggg catcgcctac ggcggcgatt acacgcccga acagtggccg cgcgagacct
     5041 ggcacgacga tgtcgcgctc atgcgcgagg ccggcgtatc catggtcagc atcggcatct
     5101 tttcgtgggc gctcctcgaa accagcgagg gcgtcttcga cttcgactgg cttgacgacc
     5161 tgatcgagct gctgcacgac aacggcatcg ccgtcgacct cggcacaccg acagcctcgc
     5221 cgccggcgtg gttcttcacg cagcatccgg atgcccgtcc cgtcagccgc gacggagtac
     5281 ccatgggctt cggcgctcgt ggcatggcgt cgccgtcgtc acccgagtac cgcgaggcgt
     5341 cggtgcgcgt tgcggggcgc ctcgccgaac gttatggcga gcatccggcg gtcgtcatgt
     5401 ggcacgtgca caacgagtac ggccagcccg tcggggagga ttactcggcc cacgcggtgc
     5461 tcgcgttccg ccagtggttg gaggcgcgct acggcacgat cgccgagctc aaccgcgtct
     5521 ggggcacggc gttctggggg cagcggtact ccgactggca cgaggtgagt gtgcccgccg
     5581 ccgcgctgtc ggtggtgaac ccggcgcagc gcctggactt cgcgcgcttc actgatcacc
     5641 agttgcgcca gtgctacatc gccgagcgcg acgcgatccg ggctcacgcc gcgcagccga
     5701 tcaccaccaa cttcatggcg aactcgagct gggtcaccga cctgtgggcg tggggccgcg
     5761 aggtcgacat cgtctcgga
//
LOCUS       DAOSOU010000134         5748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14135_length_5748_cov_6.118918, whole genome shotgun sequence.
ACCESSION   DAOSOU010000134 DAOSOU010000000
VERSION     DAOSOU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14135_length_5748_cov_6.118918"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..740)
                     /locus_tag="PLW15_07965"
     CDS             complement(72..740)
                     /locus_tag="PLW15_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34365.1"
                     /translation="MLPLTVIIPWRAQATRLAAFDAVVDWYRSNIPGVDIMTIDSDET
                     VFNLSRCRNLGIDAISDRDRVVVINDADTIPQLAALSEAVAAAATSGLVHLPYTEYHW
                     LGSAGTAQYADGMPLEDCDFELVRGACSGVYVTTPASWFSHGGQDERFRGWGFEDAAW
                     HLAHETLLGAPPQRHVGSVFALHHEPQLREGPQYDANANLMQQYRDAARDPAAMRSLV
                     FATP"
     gene            802..1245
                     /locus_tag="PLW15_07970"
     CDS             802..1245
                     /locus_tag="PLW15_07970"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HOT34366.1"
                     /translation="MSNSADLLGPVEFLVIEFPGGAVSGEGFAKLVSLVDAGHILVLD
                     LEFVAKSATGEVTVVDASTFGVVDGVDLGDFAGASSHLIDGDDVADAGAMIAPGSVAA
                     VLVYEVLTVLPLIATWEKSGASLVAAGAIEFEDLDAAITAAETRD"
     gene            1248..>1456
                     /locus_tag="PLW15_07975"
     CDS             1248..>1456
                     /locus_tag="PLW15_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOT34367.1"
                     /translation="MSRLLRTAAHAHVASSVIGRNQRRQQQRWAQQGQAAAPAAPAPA
                     AAPAAPAGGDMDAKIAQLKQLGELR"
     assembly_gap    1457..1556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1557..2020)
                     /locus_tag="PLW15_07980"
     CDS             complement(<1557..2020)
                     /locus_tag="PLW15_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT34368.1"
                     /translation="MDLTERRGMPGLAYPEEYLRRLAGDLTEKFAGVFNSETIERYVL
                     ESYTGLLRTSTVRTHLASRTIHFATDRLTALAQAKGAIERTVPEVLFVCEQNAGRSQM
                     AAVLTAQLSQGRVHVRSAGSAPGKDLNPAVVEAMAELGLSMTEEFPKPLTDDV"
     gene            complement(2023..2436)
                     /locus_tag="PLW15_07985"
     CDS             complement(2023..2436)
                     /locus_tag="PLW15_07985"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT34369.1"
                     /translation="MTEATKPTVLFVCVHNAGRSQMAAGYLKALAGDRVEVLSAGSEP
                     KDQINPVAVEAMAEEGIDIANNVPKILTTEAVKDSDVVITMGCGDTCPIFPGKRYEDW
                     ELDDPAGQGIEAVRPIRDDIKRRIEVLLAEILPAA"
     gene            complement(2489..3421)
                     /locus_tag="PLW15_07990"
     CDS             complement(2489..3421)
                     /locus_tag="PLW15_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT34370.1"
                     /translation="MHQEPTARGLGRADAERIARSLRALADPTRVQLLALIVESPDAR
                     RSVTDLADALGLTQPTVSHHLRVMADEGLVERSQDGRLAWYSIAPTRMTEVVEAMRDD
                     PVAAAVAPPVLERIASDLATRFAGTLSPETVDRYVRESYHLLAERAGITRYLPSLTAR
                     FAGDRLDALTRVEAPSQSPSVLFVCVQNAGRSQLASAILRSLAGDRVRVLTAGSEPAG
                     SVNPLVVAALDEIGVPLGGEFPKPLTDEVVRAADVVITMGCGDACPVYPGRRYLDWEL
                     DDPAGLSMDGVRRVRDEIVVRVRALLAELGVNGA"
     gene            3614..3970
                     /locus_tag="PLW15_07995"
     CDS             3614..3970
                     /locus_tag="PLW15_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT34371.1"
                     /translation="MSTATLLPLTVKDAAACCAPLAREPLGAEQAEDLARSLKAIADP
                     ARLRLVSLIAASEGQEACVCDLTDLLDLGQPTVSHHLKVLMDAGFLTRSKRGTWAYYA
                     LVPGSLDALSRLLVTT"
     gene            3967..4647
                     /locus_tag="PLW15_08000"
     CDS             3967..4647
                     /locus_tag="PLW15_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOT34372.1"
                     /translation="MIRRASAEFIGSALLAALVIGSGIAAQTLTPGDTGLQLLENAFA
                     TALGLPVLILIFATVSGSHFNPVVSLVDAALGHRSWRDVAAYMPAQIIGCLAGAILAN
                     LMFGLPAVSISTTDRFSWPHLLAEVIATAGLVLVIFALARTGRGSVIPWAVGAYIGAA
                     YFFTSSASFANPAITIGRTISDTFAGIAPASAPGYIAAQLVGGALGLLLVRGFFGRSA
                     QAAGHDQQ"
     gene            complement(4585..>5748)
                     /locus_tag="PLW15_08005"
     CDS             complement(4585..>5748)
                     /locus_tag="PLW15_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34373.1"
                     /translation="VSLLADFVYEGARSVTGPVLALLGATGLVVGIVTGVGEAASLLL
                     RLVSGPLADRTQRFWAWTIAGYAITVISVPLLGYASILWVACGLVILERVGKAIRGPS
                     KDALLSYATAATGRGRGFAVHEAIDQFGAVIGPLVVAAVLAVTNTDYGPALAVLALPG
                     VLALGLVVWLRARVPHPIDYEVDPKPHVSLTLPTERVRLPRAFWLYAAFTATTVLGFA
                     TFGIISFHIVEAGLLEPVWVPVLYAGVMVVDALAALATGWAYDRYGARVLFALPVVAA
                     SVPLFAFGSTAAAVIVGSLLWGMALGIQESTLRATVADLIPTRRRSTAYGVFAAVVGA
                     TTAGGGALAGALYDVSVPLLVAITIGVQAVAVVLLLVVTRSLRRPAEEPAHEQ"
BASE COUNT          867 a         1937 c         1981 g          863 t
ORIGIN      
        1 gggccgctcg ctgcatccct gcagaacact ccggggcgcg accgcgccta ccgcgcggtg
       61 acggcgcgcg gctagggcgt cgcgaagacc agcgagcgca tggccgcggg gtcgcgtgcg
      121 gcgtcgcggt actgctgcat gaggttcgcg ttggcgtcgt actgcggccc ttcgcgcagc
      181 tgtggctcgt ggtgcagggc gaagaccgat ccgacgtggc gctgcggggg tgcaccgagg
      241 agagtctcgt gggcgaggtg ccacgcggca tcctcgaagc cccagccgcg gaagcgctcg
      301 tcctggccgc cgtggctgaa ccagctggcg ggggtggtca cgtagacgcc cgagcatgcg
      361 ccgcgcacga gctcgaagtc gcagtcctcg agtggcattc cgtcggcgta ctgggcggtg
      421 ccggccgagc cgagccagtg gtactcggtg tagggaaggt gcacgagacc cgatgtcgcg
      481 gcggccgcga ccgcctcgct gagtgccgcg agctgcggga tcgtgtctgc gtcattgatc
      541 accacgaccc ggtcgcggtc gctgatcgca tcgatgccga ggttgcggca gcgcgacagg
      601 ttgaagaccg tctcgtcgga atcgatggtc atgatgtcca caccggggat gttcgatcgg
      661 taccagtcga cgaccgcgtc gaatgcggcg agccgggtgg cctgagcccg ccaggggatg
      721 atcacggtca gcggcagcat cagaccagcg taagcgagtt gtgcgctcga cccgaccggg
      781 cttgacacgt accatctgag catgagcaat agtgctgacc tgctggggcc ggtcgagttt
      841 ctggtcatcg aattccccgg tggggcggtg tcgggggagg gctttgcgaa gcttgtctcc
      901 ctcgtcgatg ccggacacat tctggtgctc gatctcgagt tcgtggccaa gtccgccact
      961 ggcgaggtga cggtcgttga tgcgtcgacg ttcggcgtgg tcgacggtgt cgatctcggt
     1021 gacttcgcgg gggcgtcatc ccacctcatc gacggcgacg atgtggccga tgccggggcc
     1081 atgatcgccc ccggaagcgt cgcggccgtg ctcgtctacg aggtgctgac ggtgctgccc
     1141 ctgatcgcga cgtgggagaa gtcgggcgcc tcgctcgtcg ccgccggcgc gatcgaattc
     1201 gaagacctgg atgccgcgat caccgcggcc gagacgaggg actgaccatg agcagactcc
     1261 tgcgtaccgc cgcccacgcc cacgtggctt cgagcgtcat cggccgcaac cagcgccgtc
     1321 agcagcagcg ctgggctcag cagggccagg cggcggcgcc tgctgctccg gctcccgcgg
     1381 ctgcgcccgc tgcccccgcg ggcggcgaca tggacgcgaa gatcgcccag ctcaagcagc
     1441 tcggtgaact gcgcgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgt
     1561 cgtcggtgag gggcttcggg aactcctcgg tcatcgacag accgagctcg gccatggcct
     1621 cgacgaccgc ggggttcagg tccttgccgg gtgccgaacc ggccgagcgt acatgcacgc
     1681 gaccctggga gagctgcgct gtgagcacgg cggccatctg cgagcggccg gcgttctgct
     1741 cgcagacgaa gagcacctcg ggaaccgtgc gctcgatggc gcccttggcc tgggcgagcg
     1801 cggtcagccg atcggtcgcg aagtggatgg tgcgcgaagc gaggtgagtt ctcacggtcg
     1861 acgtgcgcag caggcccgtg taggactcga gcacgtatcg ttcgatggtc tcggagttga
     1921 agacgcccgc gaacttctcc gtgaggtccc cggcgagccg cctcaggtac tcctcggggt
     1981 aggcgaggcc gggcatcccg cgacgctcgg tgagatccat ggtcaggccg caggcaggat
     2041 ctcggcgaga agaacctcaa tgcgccgctt gatgtcgtcg cggatcgggc gcacggcctc
     2101 gatgccctgc ccggccgggt cgtcgagctc ccagtcctcg tagcgcttgc ccgggaagat
     2161 cgggcaggtg tcgccgcagc ccatggtgat cacgacgtcg gagtccttga cggcctcggt
     2221 cgtgaggatc ttcggcacgt tgttggcgat gtcgatgccc tcctcggcca tggcctcgac
     2281 cgcgacgggg ttgatctggt ccttgggctc ggatcccgcg gagagcacct cgacacggtc
     2341 gccagcgagc gccttgagat agcccgcggc catctgggaa cggcccgcgt tgtgcacgca
     2401 gacgaacagc acggtgggct tggttgcttc ggtcatgctc aaagcataga tgattgtcta
     2461 tgggttcggg atgccacggc gcatgaattc acgcaccgtt caccccgagc tccgccagca
     2521 gagcccgcac ccgcaccacg atctcgtcgc gcaccctgcg cacaccgtcc atggacagcc
     2581 cggcggggtc atccagttcc caatcgaggt agcgccggcc cgggtagacg gggcaggcgt
     2641 cgccgcagcc catggtgatc acgacgtcgg cggcgcgcac gacctcatcg gtcagtggtt
     2701 tcggaaactc gccccccagg ggcacgccga tctcgtcgag cgcggcgacc acgagcgggt
     2761 tgacggatcc ggcgggctcc gagcccgcgg tcagcacccg cacccggtca ccggcgagcg
     2821 agcgcaggat cgccgaggcg agctgggagc gcccggcgtt ctgaacgcac acgaagagca
     2881 cgctcgggct ctggctcggc gcctcgactc tggtgagcgc gtcgaggcgg tccccggcga
     2941 aacgtgcggt gagcgacggc aggtaccggg tgatgccggc gcgctcggcg aggaggtggt
     3001 agctctcgcg cacgtagcgg tccacggtct cgggggagag ggttccggcg aaccgcgtgg
     3061 cgagatcgga ggcgattctc tcgagcacgg gaggggcgac tgcggcggcg accgggtcat
     3121 cccgcatcgc ctcgacgacc tcggtcatgc gggtgggggc gatcgagtac cacgcgagcc
     3181 ggccatcctg gctgcgttcg accaggccct cgtcggccat cacgcgcagg tggtgactca
     3241 ccgtcggctg ggtgaggccg agagcgtcgg ccaggtcggt gacgctgcgg cgggcatccg
     3301 ggctctcgac gatcagggcg agcaactgca ccctcgtggg gtcggccagg gcgcgcaggc
     3361 ttcgagcaat gcgctcggcg tcggcgcgcc ccagccctcg cgcggtcggc tcctgatgca
     3421 tagacaggag tctatgcgcg gctcagcagc acccggtcgg agtgcagctg ccggtcgtgg
     3481 cgagttcgag ctcgcaggcg gcgtcggttt ccatggttcc tcccttcgtg tcggtcgcaa
     3541 atatcgatga acgtcgatat tgcgagtatg cgccgctata tcgacggctg tcaatatcga
     3601 ctacgatcga tgcatgtcca cagcgaccct gttgcctctg accgtgaaag acgcggccgc
     3661 ctgctgcgcg ccgctggcgc gcgagccgct cggcgccgag caggccgaag atctcgcccg
     3721 ctcgctcaag gcgatcgccg accccgcgcg actgcgtctc gtctcgctca tcgccgcatc
     3781 cgagggccag gaggcgtgcg tgtgcgacct gaccgacctg ctcgacctcg gtcagccgac
     3841 ggtctcccac cacctgaagg tgctcatgga cgccggattc ctcacccgct cgaagcgcgg
     3901 cacgtgggcc tactatgcgc tcgttcccgg ctccctcgac gcgctctcgc gcctcctcgt
     3961 cacgacgtga tccgccgggc cagcgccgag ttcatcggca gcgcgcttct cgcggcgctc
     4021 gtcatcggat cgggcatcgc ggcccagacg ctcacgccgg gcgacaccgg cctccaactg
     4081 ctcgagaacg ccttcgccac cgcactcggc ctgcccgtgc tcatcctgat cttcgccacg
     4141 gtgagcggtt cgcatttcaa tcccgtggtc tcgctcgtcg acgccgccct cggtcatcga
     4201 tcgtggcgcg acgtcgccgc ctatatgccg gcccagatca tcggatgcct cgccggcgcc
     4261 atcctcgcca acctgatgtt cgggctcccg gccgtcagca tcagcaccac cgaccggttc
     4321 agctggccgc acctgctcgc cgaggtgatc gcgacagccg gactcgtgct cgtgatcttc
     4381 gcgctcgcgc gcaccggtcg gggcagcgtc atcccgtggg cggtcggcgc gtacattggc
     4441 gcggcctact tcttcacgag ttctgcgagc ttcgccaacc ccgccatcac gatcgggcgc
     4501 acgatcagcg acacgttcgc gggcatcgct ccggcttcgg cgcccggcta catcgccgcc
     4561 caactcgtgg gcggcgcgct cgggctactg ctcgtgcgcg ggttcttcgg ccggtcggcg
     4621 caggctgcgg gtcacgacca gcagtagcac gacggccacg gcctgcacgc cgatggtgat
     4681 ggccaccagc agaggcaccg acacgtcgta gagcgcgccg gcgagtgcgc caccaccggc
     4741 cgtcgttgcg cccaccacgg ccgcgaacac cccgtaagcg gtgctccgcc gacgggtcgg
     4801 aatgaggtcg gcgaccgtcg cccgcagcgt cgactcctgg atgcccagcg ccatgcccca
     4861 cagcagcgag cccacgatga cggcggccgc cgtgctgcca aaggcgaaca agggcacgga
     4921 tgccgcgacc accggcagag cgaacagcac ccgcgccccg taccggtcgt acgcccaccc
     4981 ggtcgcgagt gccgcgaggg cgtcgacgac catgactccc gcgtagagca caggaaccca
     5041 caccggctcg agcagtccgg cctccacgat gtggaacgag atgatgccga acgtcgcgaa
     5101 gccgagcacg gtcgtggcgg tgaacgcggc gtacaaccag aacgcccgcg gcagccgcac
     5161 gcgctcggtg ggaagggtga gtgagacgtg cggcttcggg tcgacctcgt agtcgatcgg
     5221 atgcggcacc cgcgcgcgca gccagaccac gagcccgagc gcgagcaccc cgggcagcgc
     5281 gagcaccgcg agagcgggtc cgtagtcggt gttggtgacg gcgagcacgg cggcgaccac
     5341 gagcggtccg atcacggcgc cgaactggtc gatggcctcg tgcacggcga agccgcgccc
     5401 ccggccggtt gcggccgtgg cgtaggagag cagggcatcc ttcgacgggc cgcggatggc
     5461 cttgcccacc cgctcgagga tcaccagacc gcaggcgacc cagagaatcg aggcgtagcc
     5521 gagcagcggc acgctgatga ccgtgatcgc gtatccggcg atcgtccacg cccagaagcg
     5581 ctgcgtgcga tcggcgagag gtcccgagac cagccgcagc agcagcgacg cggcctcacc
     5641 cactcctgtg acgatgccca ccacgaggcc ggtcgcgccc agcagggcga gcacgggacc
     5701 tgtgaccgag cgtgcaccct cgtagacgaa gtcggcgagc aggctcac
//
LOCUS       DAOSOU010000135         5716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14249_length_5716_cov_5.336160, whole genome shotgun sequence.
ACCESSION   DAOSOU010000135 DAOSOU010000000
VERSION     DAOSOU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14249_length_5716_cov_5.336160"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2577
                     /locus_tag="PLW15_08010"
     CDS             <1..2577
                     /locus_tag="PLW15_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT34374.1"
                     /translation="SEVASAGPSAADDEFVELLNTGTEPTDISGWQLYRCTASGRLTP
                     ATRELTVASGTTLAPGARFVIGGGHFTGTPDARLTHSLADVTFGVLLQADAGRLVDRL
                     AISSHADSACQEGDSKLPAVLDAVSNQSYQRSGQRWLVAPRTPGAANATVAGPAVGGA
                     FSYTTGQVAISEVATDPTPEGMPAGTEQRNYIELGNYGTTVADIGGWTVRRCEADGTR
                     SRDVQFTVPEGTRLAPGSVYLAAREGTAAASHADITYATSLNFLGTGLWVSDADGQRV
                     DSVGIYAANELDASNVTASPCTKGAALTTYQPDRLQQETFQRTRFTGVDRDDFVTWEA
                     TPGVLDLRDWVDPTERVVTSSTAALPAARRAFGTHQLAVDTDVVPIEAYTGVTFDGPL
                     TTLVGDGETSTDATAPADAADSGYGYPYQRFVLDATTLAVGSTVGWRGTTVDRGELQL
                     SVWSGTAWRLLASGGGELAGSLEAGDIIDGRVTLLVQDGQRGAPSVTTVRDGQLENPA
                     DYDLAITHITDTQYLTESYPEVYAQLASWIVDNAADRKIAFATHTGDLIQNWVDPDQN
                     ADRARVEFERASAIQSILDDAGLPNSVLPGNHDSKRGVDYSLFNEYFPPSRYEATDWY
                     GGSIAPGDNTANFSTFESEGAKFLMLSLPYAYGDRELDWAEQVVTSHPDFNVIVSTHE
                     HVMPKTLEVGALHSTNSRWVSRGQDLWDRVIAPNRNVIVVLSGHFHGIGQLVTEDAGG
                     LPGHTVVELLADYQEFRTHTGERATGFYRMLQLDLDGGAIAVDTRSVRLAASYSYEYD
                     YRQFLPDNGLATTPSNARPWRILDEGVQGRYSEADDEFTAYVEFQHPKLVSTDALLVG
                     AP"
     gene            2736..3182
                     /locus_tag="PLW15_08015"
     CDS             2736..3182
                     /locus_tag="PLW15_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34375.1"
                     /translation="MTARADDDALSWGGETDPSHVDGPVVSPPAVERPVGERVETPAD
                     PADPKQIPAALLITYGILGGVYLIYTIGWVTTVVRYQAVRIPFTDPLSEIMFQLGEFL
                     AIASPALWFAAVFLLSRGRKPLVRLLWILVGLVAVLPWPFVLGAWA"
     gene            3179..3592
                     /locus_tag="PLW15_08020"
     CDS             3179..3592
                     /locus_tag="PLW15_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34376.1"
                     /translation="MSDTGSISESDNPIEAVVADDAAEATRGRYGWLSLIVAFLFALF
                     YVYDLWEAVGTMFALPAFYDAYGIGAQYVPWWLLWIGVLLPIAVYVIAFIGGRRRNVF
                     GKALIFLVGLAVVAGLSLGVIAFEEILRPVALISG"
     gene            complement(3698..4081)
                     /locus_tag="PLW15_08025"
     CDS             complement(3698..4081)
                     /locus_tag="PLW15_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34377.1"
                     /translation="MDPFEIVQWVIRAVLALLFVGMGVLHFVPGPAKGMSAIIPPGIK
                     RVVPGIVLVRFTGVCEIAGGIGMLVPGLHVVAGIALIVFLVAVFPANAYASRYPERFG
                     RAAIPFWPRLFGQLALIALIVVAIV"
     gene            4194..>5716
                     /gene="serA"
                     /locus_tag="PLW15_08030"
     CDS             4194..>5716
                     /gene="serA"
                     /locus_tag="PLW15_08030"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOT34378.1"
                     /translation="MAKPIVLIAEELSPATVDALGPDFDVRSVDGTDRPALLAALADA
                     NAILIRSATNVDAEAIAAAPHLKVVARAGVGLDNVDIKAATAAGVMVVNAPTSNIISA
                     AELTVGHILSLARHIPVAHASLAEGLWKRSKYTGVELYEKTVGIIGLGRIGALITERL
                     QAFGMTVVAYDPYVTSARAQQLGVTLLSLDELLKASDFITIHMPKTPETTGMIGTDQF
                     ALMKPTAFIVNVARGGLIDEDALYAALKSNRIAGAGLDVFVSEPPTGSPLLELDNVVL
                     TPHLGASTDEAQEKAGVSVARSVRLALDGELVPDAVNVAGGAIDPSVRPGIPLMEKLG
                     QVFAGLADSPITSIDVEVYGEIVEFDVNVLKLAALKGIFSNIVSESVSYVNAPLLAEA
                     RGITVRLSTDATSSEYRNLLRISGSLSSGATISVSGTLTGTKQTQKIVEINGYDVEVP
                     VAEHLIVMIYTDRPGIVAIYGKEFGEAGINIAGMQIARQSKGGQALSVLTVDSPVPD"
BASE COUNT          921 a         2087 c         1829 g          879 t
ORIGIN      
        1 tccgaggtcg cctcggcagg tccgagcgcg gccgacgacg agttcgtcga actgctcaac
       61 accggcacgg agccgaccga catctctggc tggcagctct accgctgcac cgcgtcgggc
      121 cggctcaccc ccgcaacccg cgagctcacc gttgcgtccg gcaccacgct cgcgcccggg
      181 gcgcgcttcg tgatcggcgg cggccacttc acgggcacgc cggatgcccg cctcacgcac
      241 tcgctggccg acgtcacctt cggggtgctg ctgcaggccg acgccggtcg actcgtcgac
      301 cgtctcgcga tctcgtcgca cgcggacagc gcgtgtcagg agggtgacag caagctgccc
      361 gccgtgctcg acgcggtctc gaaccagtcg taccagcgct ccgggcagcg ctggctggtc
      421 gcgccgagaa ctccgggcgc cgccaacgcc accgtggcgg gccccgccgt cgggggcgca
      481 ttcagctaca cgaccggcca ggtcgcgatc agcgaggtcg ccaccgaccc cacccccgag
      541 ggcatgccgg cgggcacaga gcagcgcaac tacatcgagc tcggcaacta cggcacgacc
      601 gtcgccgaca tcggtggctg gaccgtccgc cgctgcgagg ccgacggcac gcgcagccgc
      661 gacgtgcagt tcaccgtgcc ggagggcacc cggttggcgc ccgggtcggt gtacctcgcc
      721 gcgcgggagg ggactgcggc ggcatcccac gccgacatca cctacgcgac gtcgctcaac
      781 ttcctcggca ccggcctctg ggtcagcgac gcggacggcc agcgggtcga ctcggtgggc
      841 atctacgcgg ccaacgagct cgacgccagc aacgtgactg cgagtccgtg cacgaaaggc
      901 gcggccctga cgacctacca gcccgaccga ctgcagcagg agaccttcca gcgcacgcgg
      961 ttcaccggcg tcgaccgtga cgacttcgtc acctgggagg ccacgccggg cgtgctcgac
     1021 ctgcgcgact gggtcgaccc gaccgagcgg gtggtgacct cctcgacggc agcgctcccc
     1081 gcggcgcgtc gagcgttcgg cacgcaccaa ctggccgtcg acaccgacgt cgtgccgatc
     1141 gaggcctaca cgggcgtcac gttcgacgga ccgctcacca ccctcgtcgg cgacggcgag
     1201 acgtcgacgg atgccacggc ccccgccgat gccgccgact cgggctacgg ctatccgtac
     1261 cagcgtttcg tgctcgacgc cacgacgctg gctgtgggct ccacggtggg gtggcgcggc
     1321 accaccgtgg atcgcggcga gctccaactg tcggtctggt cgggcacggc gtggcggctg
     1381 ctcgcctcgg gcggtggcga gctcgccggg tcgctcgagg ccggtgacat catcgacggt
     1441 cgggtgacgc tgctcgtgca ggacggccag cgcggtgccc cgagcgtgac caccgtgcgc
     1501 gacggccagc tcgagaatcc cgccgactac gacctggcga tcacccacat caccgacacc
     1561 cagtacctca ccgagagcta cccggaggtg tacgcgcagc tcgcgagctg gatcgtcgac
     1621 aacgccgccg accgcaagat cgccttcgcc acccacaccg gtgacctcat ccagaactgg
     1681 gttgaccccg accagaacgc tgaccgagcg cgggtcgagt tcgagcgcgc atccgccatc
     1741 cagtcgatcc tggatgacgc gggccttccc aacagcgtgc tgcccggcaa ccacgacagc
     1801 aagcgcggcg tcgactacag cctcttcaac gagtacttcc cgcccagccg ttacgaggca
     1861 accgactggt acggcggctc gatcgcgccg ggagacaaca ccgcgaactt ctcgaccttc
     1921 gagtccgagg gagccaagtt cttgatgctg agcctgccgt acgcctacgg cgaccgcgag
     1981 ctcgactggg ccgaacaggt ggtgacctca caccccgact tcaacgtgat cgtgtccacc
     2041 cacgagcacg tcatgccgaa gacgctcgag gtcggcgccc tgcactcgac gaactcgcgc
     2101 tgggtctccc gtgggcagga cctgtgggat cgggtgatcg cccccaatcg caacgtcatc
     2161 gtggtcctga gcggccactt ccacggcatc gggcagctcg tcaccgagga tgccggcggc
     2221 ctgcccggtc acaccgtggt cgaactgctc gccgactacc aggagttccg cacccacacc
     2281 ggcgagcggg cgaccggctt ctaccggatg ctccagctcg acctcgacgg tggggcgatc
     2341 gctgtcgaca cccgctccgt tcgcctcgcc gctagctaca gctacgagta cgactaccgc
     2401 cagttcctgc ccgacaacgg actggccacg acgccgtcga atgcccggcc gtggcgcatc
     2461 ctcgacgagg gggtgcaggg gcggtactcg gaggcggacg acgagttcac cgcgtacgtc
     2521 gagttccagc atccgaagct cgtttcgacc gatgcccttc tggtgggcgc cccgtaggat
     2581 tccctcatgg tcacgaccgc ggtgcacgtc agggcgggca acatcgaccg cttcgaatgg
     2641 gcggtcgcca gggctccgtt tgctgatgct gcgttccctg aggctctcga ggggacgcct
     2701 ccgttcatcg aggtacgact ggcacgaggc accgcgtgac cgcccgcgcc gacgacgacg
     2761 ccctctcctg gggcggcgag accgacccga gccacgtcga tggtcctgtg gtctcgcccc
     2821 cggcggtcga gcgcccggtg ggcgagcgcg tcgagacccc agccgacccc gccgacccca
     2881 agcagatccc cgcggccctg ctcatcacct acggaatcct gggcggggtc tacctgatct
     2941 acacgatcgg ctgggtcacg accgtcgtgc gataccaggc cgtgcgcatc ccgttcaccg
     3001 acccgctcag cgagatcatg ttccagctgg gggagttcct cgcgatcgcg agcccggcgc
     3061 tgtggttcgc ggcggtgttc ctgctcagcc gtgggcgcaa gccgctggtg cgcctgctct
     3121 ggattctcgt cggcttggtc gcggtgctgc cgtggccctt cgtgctggga gcgtgggcat
     3181 gagcgacacc ggttcgatct ccgagtcaga caacccgatc gaggcggtgg tcgcggatga
     3241 cgcggccgag gccactcgtg gccgttacgg ctggctcagt ctgatcgtcg ccttcctctt
     3301 cgccctgttc tacgtctacg acctgtggga ggcggtcggc accatgttcg cgctgccggc
     3361 tttctatgac gcgtacggca tcggcgccca gtacgtgccg tggtggctgc tctggatcgg
     3421 cgtgctgctg ccgatcgcgg tgtatgtcat cgcgttcatc ggtggccgtc gccgcaacgt
     3481 cttcggcaag gccctgatct tcctcgtggg cctggctgtc gtcgccggtc tctcgctggg
     3541 cgtcattgcc ttcgaggaga tcctgcgccc ggtcgcgctc atctccggct gaccctcgcg
     3601 accctcacgt cgcgtgaggt gcggcaaacg taccttcttg gttgccggaa gggtgctatt
     3661 gccgcaactc acgacgagcc gggcggggac acgtgaccta gacgatcgcg accacgatca
     3721 gcgcgatcag cgccagctgg ccgaacagcc gcggccagaa cgggatggcc gcccggccga
     3781 aacgttccgg gtagcgggac gcataggcat tggccggaaa caccgccacg aggaacacga
     3841 tcaacgcgat tcccgccacg acgtgaaggc caggcacgag catcccgatg ccgccggcga
     3901 tctcgcacac cccggtgaac cgcacgagca cgattcccgg caccacccgc ttgatgcccg
     3961 gcgggatgat cgcactcatg cccttcgccg gcccgggcac gaagtgcagc acccccatac
     4021 cgacgaagag caacgccaac actgcgcgga tcacccactg cacgatttcg aaaggatcca
     4081 cgttcttatg gtcgcacccg cgtcacactg acgcggcgcg gctggcggag ccggtaaagt
     4141 tgcccgggtt gcgcgatgcg caccgacctc tcgacgacaa aggacttttc tccgtggcca
     4201 agccgatcgt gctgatcgcc gaagaactct cgcccgctac cgtcgacgcc ctcgggcccg
     4261 acttcgacgt acgctccgtc gacggcaccg accgcccagc gctgctggcc gcgctcgctg
     4321 acgccaacgc gatcctcatc cgttccgcga ccaacgtcga tgccgaggct atcgccgcag
     4381 caccgcacct caaggtggtc gcccgcgccg gggtcggcct cgacaacgtc gacatcaagg
     4441 ccgcgaccgc cgccggcgtg atggtcgtga acgcgcccac ctccaacatc atctccgcgg
     4501 ccgagctcac ggtcgggcac atcctgagcc tcgcccggca catcccggtc gcacacgcga
     4561 gtctcgccga gggtctctgg aagcgctcca agtacaccgg cgtcgagctc tacgagaaga
     4621 ctgtcggcat catcggcctc ggccgcatcg gcgctctcat cacggagcgc ttgcaggcct
     4681 tcgggatgac cgtggtcgcc tacgacccct acgtcaccag cgcacgcgcc cagcagctcg
     4741 gcgtcaccct gctgagcctc gacgagcttc tgaaggccag cgacttcatc acgatccaca
     4801 tgccgaagac gcccgagacc acgggcatga tcggcaccga ccagttcgcg ctcatgaagc
     4861 cgacggcctt catcgtcaac gtcgcccgcg gtggactcat cgacgaggac gcgctctacg
     4921 cggccctcaa gtcgaaccgc atcgccggcg caggcctcga cgtgttcgtg agcgagccgc
     4981 ccaccggctc gccgctgctc gagctcgaca acgtcgtgct caccccccac ctcggcgcgt
     5041 ccaccgacga ggcgcaggag aaggccggtg tgtcggtggc ccgctcggtc cgtctcgccc
     5101 tcgatgggga gctcgtgccc gacgccgtca acgtcgccgg tggcgcgatc gacccgagcg
     5161 tgcggccggg catcccgctc atggagaagc tgggccaggt cttcgctggc ctcgccgaca
     5221 gcccgatcac ctcgatcgac gtggaggtct acggcgagat cgtcgagttc gacgtcaacg
     5281 tgctcaagct cgccgccctc aagggcatct tctcgaacat cgtgagcgag tcggtcagct
     5341 acgtgaacgc gccgctgctg gccgaggccc gcggcatcac agtgcgcctg tcgacggatg
     5401 ccacgagctc cgagtaccgc aacctgctgc gcatctcggg gtcgctgtcg tccggcgcaa
     5461 ccatctccgt gtcgggaacg ctcactggca cgaagcagac acagaagatc gtcgagatca
     5521 acggctacga cgtcgaggtg ccggttgccg agcacctcat cgtcatgatc tacacggacc
     5581 gccccggcat cgtggcgatt tacggcaagg agttcggcga ggccggcatc aacattgccg
     5641 gtatgcagat tgcccgtcag agcaagggtg ggcaggcgct cagcgtgctg accgtcgact
     5701 cacccgtgcc cgacga
//
LOCUS       DAOSOU010000136         5705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14289_length_5705_cov_5.689912, whole genome shotgun sequence.
ACCESSION   DAOSOU010000136 DAOSOU010000000
VERSION     DAOSOU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14289_length_5705_cov_5.689912"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..2845)
                     /locus_tag="PLW15_08035"
     CDS             complement(50..2845)
                     /locus_tag="PLW15_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT34379.1"
                     /translation="MQPRVTAVLIARNGAQYLPKTLAALAAQTRRPDRVIPVDVSSTD
                     LSPELMLRAYPGELVRTPGRSTFGGALSHALASVPSTGDDEWLWLLGHDNAPAPGALS
                     ALLGAVEVAPSVAVAGPKLMSLDSPDLIVGFGETMTRLGRSLPVVANELDQAQHDVQS
                     DLLGVAASGMLVRRSVFTALGGFDPGLPSVDAALDFCVRARLAGHRVIGVPSARVASA
                     GPIELFGRKSLSAGAQNRIRRSAQLHRRMTYAPGITLVLHWLTLVPLAVLRSLAQLIA
                     KRPGSVGGELAAGLGAAFDGSVGRARRGIRRTRVLGWASIAALRMPPAELRERRAHDR
                     AVAQGTGAPQREGPGFFAGGGAWVVLVAAVAGFIVFGRFINAQALAGGGLVPLSATVG
                     ELWSHVGYGWRDIGAGFTGAADPFAYVLAVLGTVTFWNPSFSVVLIYLLALPLAALTA
                     WLCAARFSTRGWGPAVAAIIWTAAPPFLVSLGEGQLGAVIAHILLPALVLAVVSAARS
                     WAAAAGAALLFAVVAASAPVLVPALVLLLLVWMAARPTSIHRLVGIVIPAAALFAPLV
                     VQQLGRGNWLALFATPGHPVISATPSGLQLAIGSPSGGLHGWDAFLAGLGVPAGGSVV
                     VVVALAPLAVLALLSLFLPGSRRSIPAMLVALLGFVTAVIAAHIDVTLVGSQTTSIWV
                     APALSLYWLGLAGAVMAALESLATRATVPAFLVSIGVVALAVPLFAAAATGQTAVAES
                     NGRLLPAFVSAEAATNPSLGTLQLSPEPGGGIATTVHRGLGTTLDEQSTLDATDTAVS
                     EADARLATLAGNLASRSGFDIAAELDALQLGFVLVPDGADEPDRVRLVQALDGNRLLN
                     PIGETANGFLWHYEGLADGDAPSGPGATGTSIGTGILVGQGIIVALTLLLAIPTSRRR
                     RVRTTGASDAEEVTDSE"
     assembly_gap    2866..2965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2969..3292)
                     /locus_tag="PLW15_08040"
     CDS             complement(2969..3292)
                     /locus_tag="PLW15_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT34380.1"
                     /translation="MANNGYRTGVPDDWFVDPIKLGVPGVRQITPDDDENPLAWQSDS
                     LCAQTDPEAFFPEKGGSTRDAKKICASCEVRSRCLEYALENDERFGIWGGLSERERRK
                     LRKRA"
     gene            complement(3397..4371)
                     /gene="galE"
                     /locus_tag="PLW15_08045"
     CDS             complement(3397..4371)
                     /gene="galE"
                     /locus_tag="PLW15_08045"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOT34381.1"
                     /translation="MTWLVTGGAGYIGSHVVRALRSADLQCVVIDDLSSGHEQFVPRD
                     VPFVLGTILDGSLVLQTLQDYEVTGVIHVAGFKYAGVSVQEPLRTYEQNVTGTARLLA
                     AMESADVDRIVFSSSAAVYGTPDTDLVTEETPKNPQSPYGESKLIGEWLLADQGVARG
                     LHHTSLRYFNVVGSGDPSLYDSSPHNLFPLVFTALVEGRTPRINGNDYPTPDGTNVRD
                     YIHVVDLAASHVAAAQRLDAGQPLEPAYNLGSGDGVSVGEIMATVAEVTGIPFTPEIA
                     PRRAGDPARIVAAGDLAARDLDWRMRHTLREMVETQWIATRGASQPSE"
     gene            4477..5430
                     /locus_tag="PLW15_08050"
     CDS             4477..5430
                     /locus_tag="PLW15_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT34382.1"
                     /translation="MLKNVVLLAIPGVAPFEFGVVCEVFGIDRSDTGGPRFDFTIATA
                     TPGPVETSLGYDMMIAHDLSVAADADLIAVPAHRIEAIDERFLEVIRAAEARGAWVLS
                     VCSGAFTLAQAGILEGRGATTHWMHADRLARMYPGTRVDPDVLFVEDRKVVTSAGTAA
                     GIDAALHIVRKEHGAAATNVIARRMVVPPQRDGGQSQYIESPVPAVRGDSFARVTEWM
                     LEHLDEDLTVDQLARRALMSGRTFARRFRADTGATPAAWLNRQRLIRAQQLLEESDLG
                     LEQIAQQTGFGTAAVMRHHFIKVLQTSPQAYRRAFGVREAG"
     gene            complement(5463..>5705)
                     /locus_tag="PLW15_08055"
     CDS             complement(5463..>5705)
                     /locus_tag="PLW15_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34383.1"
                     /translation="GGAWALAPILANGQPTYSVSSLEQIEVAGADAAAWGCGDGCYAF
                     FSIGGSLVSLYSGDVYDGSAFTPTFEGFVAEVVAAG"
BASE COUNT          948 a         1876 c         2006 g          775 t
ORIGIN      
        1 ctcctgctcg gggaacaggt cgggttgggc cgcgggcgac agttcggtgt cactcactgt
       61 ccgtcacctc ctccgcgtcg ctggcaccgg tcgtgcgcac gcgtcggcgc cgcgaggtgg
      121 ggatcgccag taagagggtg agggcgacga tgatgccctg cccgacgagg atgcccgtgc
      181 cgatggatgt acccgtcgcg cccggcccgc tcggcgcatc gccgtcggcg agcccctcgt
      241 agtgccagag gaagccgttg gcggtctcgc cgatcgggtt gagcaggcgg tttccgtcga
      301 gtgcctgcac gaggcgcacg cggtcgggct cgtcggctcc gtcgggcacg agcacgaatc
      361 cgagctgcag ggcgtcgagc tcggcggcga tgtcgaaccc gctgcgggag gcgaggttgc
      421 cggcgagggt ggcgagccgg gcatccgctt cgctgacggc ggtgtcggtg gcatcgagag
      481 tcgactgctc gtcgagggtc gtgccgaggc cgcggtgcac cgtcgtggcg atgccgcccc
      541 cgggctccgg gctcagttgg agggtgccga ggctggggtt cgttgccgcc tcggccgaga
      601 cgaaggcggg cagcaggcgg ccattgctct cagcgaccgc ggtctgaccc gtcgcggcgg
      661 ccgcgaacag gggcacggcg agcgcgacga cgccgatgct gacgaggaac gccggcacgg
      721 tcgcgcgggt ggcgagcgat tcgagtgcgg ccatcaccgc accagcaaga ccgagccagt
      781 acaggctgag ggcgggagca acccagatcg aggtcgtctg cgatccgacc agtgtcacgt
      841 cgatgtgggc ggcgatgacg gccgtcacga atccgagcag cgcgacgagc attgcgggaa
      901 tcgaccgacg cgagccaggc aggaacagcg agagcagcgc gagaacggcg agaggcgcga
      961 gggccaccac cacgacgacc gacccgcccg ccggcactcc cagcccggcc aggaaggcat
     1021 cccagccgtg caggccgccg gacggcgagc cgatggccag ctgcagtccg ctcggagtag
     1081 ccgagatcac cggatgcccg ggcgtcgcga acagcgcgag ccagttgccg cgcccgagct
     1141 gctgtacgac cagtggcgcg aacagcgcgg ccgcggggat cacgatgccc acgagacggt
     1201 ggatgctggt cggccgtgcc gccatccaga ccagaagcag cagcacgagg gccggaacga
     1261 gcaccggcgc acttgcagcg acaacagcga acaggagcgc tgctcccgcc gcggcagccc
     1321 agcttcgcgc ggcggagacg accgccagca cgagcgcggg cagcaggatg tgggcaatga
     1381 ccgcgccgag ctgaccctcc cccagcgaga cgaggaaggg cggggctgcg gtccagatga
     1441 tcgcagcgac ggccggtccc caaccccggg tggagaatct ggccgcgcac aaccatgcgg
     1501 tgagcgctgc gagcggcagc gcgagcaggt agatcagcac gacactgaac gaaggattcc
     1561 agaatgtgac ggtgccgagc acggcgagca cgtacgcgaa cggatcggcg gcccccgtga
     1621 agcccgcgcc gatgtcacgc cagccgtagc ccacgtgtga ccacaactcg ccgactgtcg
     1681 cgctcagagg cacgagtccg ccccccgcca gcgcctgagc gttgatgaag cgcccgaaga
     1741 cgatgaaccc ggcaaccgcg gcgaccagca ccacccaagc gccgccaccg gcgaagaatc
     1801 ccggaccttc gcgctgcggg gcacccgtgc cttgggcgac agctcggtcg tgggctcgac
     1861 gttcgcgcaa ctcggcgggg ggcatccgca gggctgcgat cgaggcccag ccgagcacac
     1921 gggtgcgacg gatgccacgg cgcgcccgac cgaccgagcc gtcgaacgcc gccccgagcc
     1981 ccgccgcgag ctcgccgccc acgctgccgg ggcgcttggc gatgagttgg gcgagcgatc
     2041 gcagcaccgc gagcggcacg agcgtcagcc agtgcaggac gagggtgatg cccggggcgt
     2101 aggtcatgcg ccggtgcagc tgcgccgagc gacggatgcg gttctgggcg ccagcagaca
     2161 gcgacttgcg accgaacagt tcgatgggtc cggcgctggc aacccgggcc gagggcaccc
     2221 cgatgacacg gtggccggcc aggcgggccc gcacgcagaa gtcgagcgcg gcatccacgc
     2281 tgggaaggcc gggatcgaag ccgccgaggg cagtgaatac tgagcggcgc acgagcatgc
     2341 ccgatgcggc gacaccgagc aggtcgctct gcacatcgtg ctgggcctgg tcgagctcgt
     2401 tcgcgacgac cggcagcgag cgccccaggc gggtcatggt ctcgccgaaa cccacgatga
     2461 ggtcgggcga atcgaggctc atgagcttgg gtccggcgac ggcgaccgac ggggcgacct
     2521 cgacggcacc gagcagcgcc gagagcgccc cgggggccgg cgcgttgtcg tgaccgagca
     2581 gccagagcca ttcgtcgtcg ccggtcgagg gaacggaggc gagggcgtgc gagagtgccc
     2641 cgccgaaggt gcttcgaccc ggcgtgcgca cgagttcacc ggggtatgcc ctcagcatga
     2701 gctcgggcga cagatccgtc gagctgacgt cgacggggat gacgcgatcg ggtcgccgag
     2761 tctgcgccgc cagtgcggct agcgttttcg gtaggtactg cgccccgttt cgggcgatga
     2821 gcaccgcagt gactctcggc tgcataacgc tttcgagact atgcgnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnaactc aggcgcgctt gcgcagcttg cgccgctcac
     3001 gctccgagag accgccccag atgccgaacc gctcatcgtt ctcgagcgcg tactcgaggc
     3061 agcggctgcg cacctcgcac gacgcgcaga tcttcttggc gtcgcgggtc gagccgccct
     3121 tctcagggaa gaaggcttcg gggtcggtct gagcgcacag ggagtcggac tgccacgcga
     3181 gcgggttctc gtcgtcgtcg ggcgtgatct ggcgaacgcc gggaacaccc agcttgatgg
     3241 ggtcgacgaa ccagtcgtca ggaacaccgg tgcgataccc gttgttcgcc atgtgtccgt
     3301 ctcccctccg cgaatgacac aaccgccagc gcgttagtga taattacacc ggtgtagttc
     3361 cgctacgtca agtcgcggat gataaatggc tcaagttcac tctgagggtt gtgacgcgcc
     3421 gcgtgttgct atccattgcg tctcgaccat ctcgcgcagg gtgtggcgca tccgccaatc
     3481 caggtcgcgc gccgcgaggt cgcccgcggc gacgatgcgg gcgggatcgc cggcacgccg
     3541 gggagcgatc tcgggtgtga acgggatgcc ggtgacctcg gccaccgtcg ccatgatctc
     3601 gccgaccgac accccgtcgc cgctgcccag gttgtaggcg ggctcgagcg gttggcccgc
     3661 atccagccgc tgggccgcgg cgacgtggga ggccgcgagg tcgaccacgt ggatgtagtc
     3721 gcgcacgttc gtgccgtccg gagtcgggta gtcgttgccg ttgatgcgcg gggttcgacc
     3781 ctcgacgagc gccgtgaaca cgagcgggaa caggttgtgc gggctcgagt cgtagaggga
     3841 ggggtcgccg gagcccacga cgttgaagta gcgcaggctc gtgtggtgaa ggccgcgcgc
     3901 cacgccctgg tcggcgagca gccactcccc gatcagcttg gactccccgt acggtgactg
     3961 cgggttcttg ggggtctctt ctgtgacgag gtcggtgtcg ggtgttccgt agacggccgc
     4021 gctcgaggag aacacgatgc gatcgacgtc ggcggactcc atcgcggcga ggagtcgcgc
     4081 ggtgcccgtc acgttctgct cgtaggtgcg caggggctcc tgaacggaga cgcccgcgta
     4141 cttgaacccc gcgacgtgga tgacgccagt cacctcgtag tcctgcaggg tctgaagcac
     4201 caggctgcca tcgaggatcg ttccgagcac gaagggcaca tcgcgcggaa cgaactgctc
     4261 gtgacccgac gacaggtcgt cgatcaccac gcactgcagg tccgccgaac gcagggcccg
     4321 aaccacgtgg gatccgatgt atccggcacc gccggtgaca agccaggtca tggcctcaac
     4381 gctatcgccg gttgagtgac acgacgtgcg tgtcgaaaca gtcgacctcg tggcagtatt
     4441 tacgtgtgca gccataacct gccatgggag gcatccatgc tgaagaacgt cgtactactg
     4501 gcgatacccg gagtcgcccc gttcgagttc ggggtcgtct gcgaggtgtt cggcatcgat
     4561 cgcagcgaca ccggcggacc gcgattcgac ttcacgattg ccaccgccac cccggggccg
     4621 gtcgagacga gtctcggata cgacatgatg atcgcccacg atctgagcgt tgcggctgac
     4681 gccgacctca tcgcggttcc cgctcaccgc atcgaagcca tcgacgagcg attcctcgag
     4741 gtgatccgcg ccgccgaggc tcgcggtgcc tgggtgctca gtgtctgcag tggcgcgttc
     4801 acactcgccc aggcaggcat cctcgagggt cggggcgcga ccacgcactg gatgcacgcc
     4861 gatcgcctcg cccgcatgta cccgggcacc cgggtggacc ccgacgtgct cttcgtggag
     4921 gatcgcaagg tcgtcaccag cgccggcacc gccgcgggaa tcgacgccgc cctgcacatc
     4981 gtgcgcaagg agcacggggc ggcagccacc aacgtcatcg cccgccgcat ggtcgtgcca
     5041 ccccagcgcg acgggggtca atcgcagtac atcgagagcc ccgtgcctgc ggtgcgcggc
     5101 gactcattcg cccgagtgac cgaatggatg ctcgaacacc tcgacgagga cctcacggtc
     5161 gaccagctcg cccggagggc gctcatgtcc gggcgcacgt tcgcgaggcg gttccgtgcg
     5221 gacaccgggg cgacccccgc ggcctggctc aaccggcaac ggctcatccg ggcgcagcaa
     5281 ctgctcgagg agagcgacct cggcctcgag cagatcgccc agcagaccgg cttcggaacg
     5341 gcggcggtga tgcggcacca cttcatcaag gtgctgcaga cctcacccca ggcctaccgg
     5401 cgggctttcg gggtgcggga ggcgggttag ggcccggaag ccacgcccca aggtcacgca
     5461 gatcagccgg cggcgacgac ctccgcgacg aacccttcga acgtgggcgt gaacgccgaa
     5521 ccgtcgtaca catcgcccga gtagagagat accaggctgc cgccgatgct gaagaaggcg
     5581 tagcatccgt caccgcagcc ccacgccgcg gcgtcggcac cggcgacctc gatctgctcg
     5641 aggctgctca cggagtaggt aggctgcccg ttcgcgagaa ttggggcgag cgcccacgcg
     5701 ccgcc
//
LOCUS       DAOSOU010000137         5658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14441_length_5658_cov_7.001606, whole genome shotgun sequence.
ACCESSION   DAOSOU010000137 DAOSOU010000000
VERSION     DAOSOU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14441_length_5658_cov_7.001606"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLW15_08060"
     CDS             <1..902
                     /locus_tag="PLW15_08060"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOT34384.1"
                     /translation="PAQAATPPTYELAEGIIWDDRAGLVRWVDLWEGRVLAGTLTDDT
                     LTVTETIVLGQAAAAVGLAHDGGLVVAAARGLAVISPDGAVSFGPDLIGERTGVRLND
                     GRADPQGRFLTGTLTFEGKGARDVGNEVLLRVSPDGGTEVLREGVRLSNGIGFSPDGG
                     TIYHADTFAKTVSTHSYGPGAFDHTEPWVPLITEFDGHPDGLTVDAAGNLWIAEWGGH
                     RVRRYSPSGELLATVSVDAAQVSCPGFVRPGLTTLAITTAQEGLTEFDDQAGAIFLAD
                     VDAVGQSPYRWAGSTAEPYWAGI"
     gene            902..1927
                     /locus_tag="PLW15_08065"
     CDS             902..1927
                     /locus_tag="PLW15_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT34385.1"
                     /translation="MRALVVTAPRQAAVLEVEDPVASPGELLVEVERVGICGTDVELY
                     TGEMAYHDQGVTHFPLQLGHEWTGRVVAVGSAEDESWLGRRVTGDTMLGCGHCDYCLS
                     GHHHVCPDRFEVGIRDGWPGALAERVLIPTRYAYAIPESVSLAAAALVEPGGNSLRAA
                     RAAAIEPGHRVLVLGSGTIGLLAALFARAEGAEVHIAGVREGSLELARSLGVHSTLRL
                     DEIGDRENEYDSVIEATSVETMPSLAVRLAKPAGRVVYIGLSSTPSLVDSRHIALKDI
                     TAVGILSASPGLAGAIQSFAAGAVVPDALVSEVIPLDDVPSRLEGRRGATAGPGPKVH
                     VDPRMRS"
     gene            1924..2727
                     /locus_tag="PLW15_08070"
     CDS             1924..2727
                     /locus_tag="PLW15_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT34386.1"
                     /translation="MNDNEERLVGADRVIAVLTELAEHPLGVTLDELAGILNSSKPTV
                     HRALATLRRAGLADLMGRGVYVLGDEFVRLALRNLDGRPETIRIQPLLEELAEVTGET
                     SHYAILAGTEIVYRAKADPPRGAVRLTSVVGGRNPAHSTSVGKALLSQRPHSVSELTD
                     HFGPGPLEARTPNTLTSIPDLAADLDAIARRGYAIDDEENELGITCVAVPVFTDSSPT
                     PVGAISISAVTFRCPKEQLVGLVPTLRATIQRHLGPGSLGPGVAGPETN"
     gene            complement(2724..3533)
                     /locus_tag="PLW15_08075"
     CDS             complement(2724..3533)
                     /locus_tag="PLW15_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT34387.1"
                     /translation="MEITGKVFVVTGGGNGMGREVVLGLIKRGARVAAVDLNEAGLAE
                     TAQLANAPEGHLTTHPLNVTDRKAVLALPAAVVKAHGQVDGLLNVAGIIQPFVKIKDL
                     DFEVIERVMNVNFWGVVNTSKAFLPLLLERPSAALLNVSSMGGFLPVPGQAAYGASKA
                     AVKLFTEALYAESQGTGLAVTVVFPGGVATHITENSGVDTSKMMAAMGDAKAPKTTSA
                     PDAARQIIEGVEKGSFRVVIGGDAKFMDRFARLNPRRATLLIADQMKSLLG"
     gene            3722..4636
                     /locus_tag="PLW15_08080"
     CDS             3722..4636
                     /locus_tag="PLW15_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT34388.1"
                     /translation="MVVRITLKDVARASGVSPTTVSFVLNNTPGQTIPELTRQRVRQS
                     ATELGYLPHSIARALREGRSRVVLLTTGRWRGGQSLDSFIAGVSGELRARGHNLVVHY
                     GEQSDESLAELLEAVSPRAVLDLAGADLGQEGSDWTDGLAPATAAQLRHLVDRGHTAI
                     ALALPSGDYGALVEIRLSQSQRAAEALGIPPVTPVVVGQSRAEAREAMRWLRHEHPQI
                     TAVAGFDDDTALLAMGALADAGVAIPETMAVIGFDDARHGAIWTPALTSVHIDAEAYG
                     RRAACAALGIEPEEWTGDPSYIVLREST"
     gene            4646..>5658
                     /locus_tag="PLW15_08085"
     CDS             4646..>5658
                     /locus_tag="PLW15_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HOT34389.1"
                     /translation="MMPRCPHCQSFAFLDWTACGSCGVELGYRYTTLSFLQAHPEGTL
                     ADGDTWRTCSNRDWGCNWLVADSEESGRCFSCRLSRRLPDRDDTIAWEKLKDTGIAKR
                     RLLVQLFELGLPVTPYYEKEGGLGFDLLSSLSGERVTIGHANGIITLDLVETLDAYRE
                     NLRVRLGEPYRTMLGHFRHEIGHYYQWVLVTSDEQWAACRALFGDERASYQDAIQRHY
                     REGAPADWADSFISEYATMHPWEDFAESFAHYLHITGTLATAAAAGVILSVDRVQGII
                     DHDVVPESDYSALGVDRMLDDWHWLSLMFNRVNRSMGQSDLYPFTLTEPVIAKLRFVH
                     ELVA"
BASE COUNT          879 a         1973 c         1944 g          862 t
ORIGIN      
        1 accccgcaca agcagcgact ccgcccacct acgaactcgc cgagggcatc atctgggacg
       61 accgggccgg cctcgtgcgc tgggtcgatc tctgggaggg ccgggtgctc gccggcaccc
      121 tcacggatga cacgctcacc gtgaccgaga cgatcgtgct gggtcaggcg gccgccgccg
      181 tcgggctcgc ccacgacggg ggtctggtgg tcgccgccgc gcgcggactt gcggtcatct
      241 cccccgacgg cgccgtctcc ttcgggcccg atctcatcgg cgagcgcacc ggggtgaggc
      301 tcaacgacgg tcgcgccgac ccgcagggcc ggttcctgac cggcacgctc acgttcgagg
      361 gcaagggcgc tcgcgacgtc ggcaacgagg tgctcctgcg ggtctcgccc gacggcggca
      421 ccgaggtgtt gcgcgagggc gtgcggctgt cgaacggcat cggcttctcg cccgacggcg
      481 gcacgatcta ccacgccgac acgttcgcga agacggtgtc cacccactcc tacgggccgg
      541 gggcgttcga ccacactgag ccgtgggtgc cgctgatcac cgagttcgat gggcatcccg
      601 acggcctcac ggtcgatgcc gccggaaacc tgtggatcgc cgagtgggga ggccaccgag
      661 tgcgccgata ctcccccagt ggtgagctcc tcgccacggt cagcgtggat gccgcgcagg
      721 tgtcgtgccc gggcttcgtg cgacccggcc tcaccactct cgcgatcacg accgcccagg
      781 aggggctcac cgagttcgac gaccaggccg gcgcgatctt cctcgccgac gttgacgcgg
      841 tgggtcagtc accgtaccgc tgggccggga gcaccgccga gccgtactgg gcagggatct
      901 gatgcgcgcc ctcgtcgtca cagctccacg ccaagccgcc gtcttggagg tcgaggatcc
      961 ggtggcctcc cccggcgagc tgttggtgga ggtcgagcgc gtgggaatct gcggcaccga
     1021 cgtcgagctg tacaccggcg agatggcgta ccacgaccag ggtgtgaccc acttcccgct
     1081 tcaattgggc cacgagtgga ccggacgggt cgttgctgtc ggttcggcgg aggatgagtc
     1141 gtggctcggc cgtcgcgtga ccggcgacac gatgctcggg tgcggccact gcgactactg
     1201 cctgtcggga catcaccacg tgtgcccgga tcggttcgag gtgggaatcc gcgacggatg
     1261 gccgggcgca cttgccgagc gcgtgctcat cccgacacgc tacgcctacg cgatacccga
     1321 gtcagtgagc ctggcggcgg ccgcactcgt ggaaccgggc ggcaactcgc tgcgcgccgc
     1381 ccgggccgcg gcaatcgaac ccggacaccg cgtgctcgtg ctggggtcgg gcacgatcgg
     1441 actgctcgcg gcgctgttcg cgcgcgccga gggggcggag gtgcacatcg ccggcgtgcg
     1501 cgagggatcg ctcgagctcg cccgatcgct cggcgtgcac agcaccctgc gactcgacga
     1561 gatcggcgat cgtgagaacg agtacgactc ggtgatcgag gcgacctcgg tcgaaaccat
     1621 gccgagtctg gcggtgcgtc tggccaagcc ggccgggcgc gtggtctaca tcggactgtc
     1681 gtcgacaccg agcctcgtcg actcccggca catcgccctc aaggacatca ccgctgtggg
     1741 aatcctgtcg gcgtcgcccg ggctcgccgg cgccatccag agcttcgccg ccggcgccgt
     1801 agtgccggat gccctggtca gcgaggtcat cccgctcgat gatgtgccca gccgcctcga
     1861 aggccgccgc ggggccaccg ccggaccggg ccccaaagtg catgtcgacc cccggatgcg
     1921 atcgtgaacg acaacgagga acgtctggtc ggagccgacc gcgtgatagc cgtgctcacc
     1981 gaactcgcgg agcacccgct cggggtgacg ctggacgagt tggcgggcat cctgaacagc
     2041 tcgaagccga cggtgcatcg cgccctggcc acgctgcgac gtgcggggct ggccgacctc
     2101 atgggtcgcg gcgtctacgt gctgggggac gagttcgtgc gattggcgct gcgcaacctc
     2161 gacggtcggc ccgagaccat ccgcatccag ccgttgttgg aagagctcgc cgaggtgacc
     2221 ggcgagacgt cgcactacgc gatcctcgcc ggcaccgaga tcgtgtaccg ggccaaggcc
     2281 gatccgcccc gcggcgcggt gcggctgacc tcggtcgtcg gcggtcgcaa tcccgcgcac
     2341 agcacgtcgg tgggcaaggc cctcctcagc cagcgaccgc attcggtgag cgagctgacc
     2401 gatcacttcg gccccggccc gcttgaggcg cgcacgccga acacgctcac gagcatcccc
     2461 gacctcgcgg ccgacctcga cgcaatcgcc cgccgggggt acgcgatcga cgacgaggag
     2521 aacgagctcg gcatcacctg cgtcgccgtt cccgtgttca cggattcctc ccccaccccc
     2581 gtgggcgcga tcagcatcag cgcggtcacg ttccgctgcc cgaaggagca actcgtcggc
     2641 ttggtaccca cgctgcgcgc gacgatccag cgccacttgg ggccggggtc actcgggccc
     2701 ggagttgccg ggcccgagac gaactagccc agaagcgact tcatctggtc ggcgatcagg
     2761 agggtcgcgc gacgcgggtt gagccgcgcg aagcggtcca tgaacttcgc gtcaccgccg
     2821 atgacgacac ggaagctgcc cttctcgacg ccctcgatga tctggcgcgc ggcatccggt
     2881 gcactcgtgg tcttgggcgc cttggcgtca cccatcgcgg ccatcatctt cgacgtgtcg
     2941 acacccgagt tctcggtgat gtgcgtggca acaccgccgg ggaacacgac ggtcaccgcg
     3001 aggccggtgc cctgcgactc cgcgtagagc gcctcggtga acagcttgac ggccgccttg
     3061 ctcgcaccgt aggccgcctg gccgggaacc ggcaggaatc cgcccatgct ggagacgttg
     3121 agcagcgctg cggacgggcg ttcgagcagc aacggcagga atgccttcga cgtgttgacg
     3181 acgccccaga agttgacgtt catcacccgc tcgatcacct cgaagtcaag gtccttgatc
     3241 ttgacgaagg gctggatgat tcccgccacg ttgagcaggc cgtcaacctg accgtgcgcc
     3301 ttgacgacgg ccgccggaag cgcgagaacc gccttgcggt cggtgacatt gagcggatgc
     3361 gtcgtgaggt gcccctcggg cgcgttcgcc aactgcgcgg tctcggcaag ccccgcctcg
     3421 ttgaggtcga cggcggcgac gcgggcgccg cgcttgatca gtccgagcac gacctcacgg
     3481 cccatgccgt ttcctccgcc ggtgacgacg aatactttgc cggtgatctc catggtgaat
     3541 cctttcgatt gctggtgcgc tggtcgtgag tcgaggagag cagcgagcct gtatggggcc
     3601 caagctacgc cgggtcgcat cctcgagtca gggccgaaag gcccacgacc cggcggcgct
     3661 cgacactggg cgccacttgc gacacgatag tcactaaatc gaattagtaa caggagggtc
     3721 cgtggtcgtg cgcatcaccc tcaaggacgt cgctcgcgcc agcggggtct cgcccaccac
     3781 ggtgagtttc gtgctcaaca acaccccggg tcagaccatc ccggagctca cgcgccagcg
     3841 ggtgcgccaa tcggcgaccg agctcggcta cctgccccac agcatcgcga gggccctcag
     3901 ggaggggagg tcgagagtgg tcctcctgac cacaggccgg tggcgaggcg ggcagagcct
     3961 cgacagcttc atcgcgggcg tgagcggcga gctgcgtgcc cggggccaca atctcgtcgt
     4021 gcactacggg gagcagagcg acgaatccct ggccgagctc ctcgaggcgg tctcgccccg
     4081 cgccgttctc gacctcgccg gcgccgacct cggccaggag gggtccgact ggaccgacgg
     4141 gctcgccccg gcgacggcgg cccagctgcg gcacctcgtc gaccgcggac acaccgccat
     4201 agccctggcc ctgccgagcg gcgactacgg cgcgctcgtg gagatcaggc tctcccagtc
     4261 gcagcgtgca gcagaggcac tgggcatccc gccggtcacc ccggtggtcg tggggcagtc
     4321 gcgcgccgag gcccgcgagg ccatgcggtg gttgcggcac gagcatccgc agatcacggc
     4381 cgtcgcgggc ttcgacgatg acactgcgct tctcgccatg ggcgccctgg ccgatgccgg
     4441 cgtcgcgatt ccggagacga tggccgtgat cggcttcgac gacgcccggc acggcgcgat
     4501 ctggacgcct gcgctgacga gcgtgcacat cgacgccgag gcgtacggtc ggcgcgcggc
     4561 ctgcgcggcg ctgggcatcg agcccgagga gtggacgggc gacccctcct acatcgtgct
     4621 gcgcgagtcg acctaggctg gcaccatgat gccccgctgt ccgcattgcc agagcttcgc
     4681 gttcctggac tggacggcgt gcggcagctg cggcgtcgag ctgggttacc ggtacacgac
     4741 gctgagcttt ctgcaggcgc atccggaggg caccctggcc gacggcgaca cctggcgcac
     4801 ctgctccaac cgcgactggg ggtgcaactg gctggtcgcc gacagcgagg aatcgggccg
     4861 gtgcttctcc tgtcggctca gtcgccgact gcccgaccgg gatgacacga tcgcctggga
     4921 gaaactcaag gacacgggca tcgccaagcg ccgcctgctc gtgcagctgt tcgagctggg
     4981 actgcccgtc accccgtact acgagaagga gggcggactc ggcttcgatc tgctctcgag
     5041 cctcagcggc gagcgcgtca ccatcgggca cgcgaacggc atcatcacgc tcgacctcgt
     5101 cgagaccctc gacgcctacc gggagaacct gcgcgtgcgt ttgggggagc cgtaccgcac
     5161 gatgctcggg cacttcaggc acgagatcgg gcactactac cagtgggtgc tcgtcacttc
     5221 agacgagcag tgggccgcct gccgtgcact gttcggtgac gagcgcgcca gctaccagga
     5281 cgcgatccag cgccactacc gcgagggcgc cccggcggac tgggccgact cgttcatctc
     5341 cgaatacgcg accatgcatc cgtgggagga cttcgcggag agcttcgcgc actacctgca
     5401 catcacggga accctcgcga ccgccgccgc cgcgggagtg atcctcagcg tggaccgggt
     5461 gcagggcatc atcgaccacg acgtcgtgcc cgagagcgac tacagcgctc tcggagtcga
     5521 tcggatgctc gacgactggc actggctcag cctcatgttc aaccgggtga accggtcgat
     5581 gggccagagc gacctgtacc cgttcactct cacggagccg gtcatcgcga agctgcggtt
     5641 cgtgcacgag ctcgtcgc
//
LOCUS       DAOSOU010000138         5616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14569_length_5616_cov_6.166517, whole genome shotgun sequence.
ACCESSION   DAOSOU010000138 DAOSOU010000000
VERSION     DAOSOU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14569_length_5616_cov_6.166517"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1251)
                     /locus_tag="PLW15_08090"
     CDS             complement(145..1251)
                     /locus_tag="PLW15_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HOT34390.1"
                     /translation="MTDSPALRKLERQVRRRLDVRMAGRRVWESLPAIIQIVSAVAAA
                     YSIAHYGLGHAVPILAVTVTINSLGFTRDARPRRVVETVIGILLGVALSDGLSLMLGK
                     GLWQLVVVLLVVFVIGRALRSNPAFALAAATPSALVVILPIPEGGPFGRTVDAAIGGA
                     VALLATALIPRDPQRAATRDRRILFSAIDQSMQSIIDCLSNADTGAGELALSRLRTTQ
                     PLVDAWTTSLDSAIAVSRISPWLRPRLPELHRDARVLTAADLTARHLRSVARRVEFLV
                     RDGVPRPALAELMTQIATGIRLLNEELDDPQLAGASRSLVGDLARRLDPAGVMPDAHV
                     TDAALVLMLRPLTVDLLAGSGMPIDEARELLPEL"
     gene            complement(1248..2432)
                     /locus_tag="PLW15_08095"
     CDS             complement(1248..2432)
                     /locus_tag="PLW15_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOT34391.1"
                     /translation="MPPASVVEPVETPAPFARRPNAVGSPPQHYGNPLGEQRALAAGS
                     AIVDLSDRAVITVTGPDRLTWLDSLTSQSLAGLAAGASAETLLLDPTGRLEHAIRVID
                     DGVTAWLLVDGAEGAPLAAWLDRMRFMLRVEVADRSADYATFGTLAPVVELVETSPSV
                     ELVETSPAQIPAAAPNGIPLIWRDPWLDVAPGGHQYAHPAQHPAAGWTYAEVLVDRAD
                     AAAAASLADSPAAGTLALEALRIAAWRPRLSTEVDEKTIPHELDWLRSAVHLNKGCYR
                     GQETVAKVHNLGHPPRRLVMLHLDGSEGVLPGPGDPVLLPSADDSAPTEVGRITSAAI
                     HHELGPIALAVIKRNVDPAADLVVAADGAQVAAAQEVIVPTDAGAEANVPRLPRLGAV
                     RR"
     gene            complement(2437..3033)
                     /locus_tag="PLW15_08100"
     CDS             complement(2437..3033)
                     /locus_tag="PLW15_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HOT34392.1"
                     /translation="MISLNADIPAELAPLSWLLGVWEGTGVVHYGVGDETKTLEFGHR
                     VSFTHDGLPHLNYNSYAWLLDDALTPHVTETGYWRLSRPAAEGDPGPGMLAGTGDKPF
                     TTASAVETLRNSDGGFDIEAAIVHPNGVSELYLGQVKGARIDLATDAVVRSASAKEYA
                     AATRLYGLVDSHLLWAWDIAALGQQLKTHASGRLARVD"
     gene            complement(3365..3622)
                     /locus_tag="PLW15_08105"
     CDS             complement(3365..3622)
                     /locus_tag="PLW15_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34393.1"
                     /translation="MTWQIWSVVPVWLAAAAAAVIIGLTSAPEARVTWLGVALAGAVI
                     VTFFIQLAIQRKEGFVVRAMASVTGAIVVLAIASAVFALIG"
     gene            3675..4373
                     /locus_tag="PLW15_08110"
     CDS             3675..4373
                     /locus_tag="PLW15_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT34394.1"
                     /translation="MAQLLILTSAVDSEVFPALGLLSHTTKQIPASPASLISAPTCDL
                     IFVDARQDLASAKSLCKILTTTGVTVPLILILTEGGLTAVSADWGVDDVILATAGPAE
                     ADARIRLALGRQALDSSSSKIQASGVVIDEASYSAKVHGRPLDLTFKEFELLRFLATH
                     PSRVFTREQLLSEVWGYDYFGGTRTVDVHVRRLRAKLGDLESLIGTVRNVGYRFNVYD
                     DDAERLTPAPVASS"
     assembly_gap    4417..4516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4517..5365
                     /gene="mshD"
                     /locus_tag="PLW15_08115"
     CDS             <4517..5365
                     /gene="mshD"
                     /locus_tag="PLW15_08115"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HOT34395.1"
                     /translation="RLARLIARARAADGHPPFSDGALAELATGAREVVWLDDVAAAVR
                     TVTDASSASVTEAEFVVDPDARRHGHGRAMLHRVLVDAPGDLLVWAHGGHPGARRLAA
                     SHGLEPVRELLHLRGEVPSGAEFAWKTHAAGEPLATLANSVHGAVDALLAVNARAFAE
                     HPEQGRLTRAEFDALAAEPWFDPEDVLLAWDGDRLAGFCWLKVEGGGSGEFYVVGVDP
                     DYQGQGWGRRLVEAGMARLASRGIREADLYVEADNAPALRLYREFGFREDSIDIQYRW
                     RSDPGH"
     gene            5411..>5616
                     /locus_tag="PLW15_08120"
     CDS             5411..>5616
                     /locus_tag="PLW15_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34396.1"
                     /translation="MDSDQSLADIGEATDSLDEYDLDESSSVDDGSLAPERYLDRELS
                     WLAFNQRVLDLAEDEMLPLLERTN"
BASE COUNT          865 a         1885 c         1988 g          778 t
ORIGIN      
        1 tggcggctcg gcgagctgac ggtcgcgtgg gaggcggtgg cgccgcgcgg gttcacgtgg
       61 tgacgcgggc cgccggtgat cgagcctgtc gagatcccgc cggttgagta gccgcgcagc
      121 ggcgtatcga aaccgctcgg agccctacaa ctccggcagc agctcgcgcg cctcgtcgat
      181 cggcatgccc gacccggcga gaagatcgac ggtgagcggg cgcagcatga gcacgagggc
      241 ggcgtcggtc acgtgggcat ccggcatcac gcctgccgga tcgaggcgtc gagccaggtc
      301 gccgaccagc gaccgggacg cgcccgcgag ctgcgggtcg tccaactcct cattgagtag
      361 gcggatgccc gtggcgatct gcgtcatcag ctcggccagg gcgggccggg gcacgccgtc
      421 gcgcacgaga aactcgaccc gccgcgcgac ggaccgcagg tggcgcgcgg tgaggtccgc
      481 ggcggtcagc acccgggcat cccgatgcag ttcgggcagg cgggggcgca gccacgggga
      541 gatgcgcgac acggcgatcg cgctgtcgag gctcgtcgtc cacgcgtcaa ccagcggctg
      601 agtagtacgc agtcgactca acgcgagctc gccggcaccg gtgtcggcgt tgctgaggca
      661 gtcgatgatc gactgcatgc tctgatcaat cgccgagaac aggatgcgac ggtcccgagt
      721 tgcggcgcgc tgcgggtccc gcgggatgag cgccgtggcg agcagggcca cagcgccgcc
      781 gatcgcggca tccacggtgc gaccgaacgg acccccttcg ggaatcggca ggatcaccac
      841 gagcgcggac ggcgtagccg ccgccagcgc gaaggccggg ttcgagcgca gcgcccgacc
      901 gatgacgaac accacaagca gcacgacgac cagctgccac aaccccttgc cgagcatgag
      961 cgacagccca tcactcaggg cgacgccgag caggatgccg atcaccgtct cgaccacgcg
     1021 ccgcggtcgg gcgtcgcgcg tgaagccgag cgagttgatg gtgaccgtca ccgcgaggat
     1081 cggcaccgca tggccgaggc cgtagtgggc gatcgagtac gcggccgcga cggcggaaac
     1141 gatctggatg atcgcgggca gcgactccca cacccgtcgg ccggccatgc gcacatcgag
     1201 gcgccggcgc acctggcgtt cgagcttgcg cagagccggg gagtcggtca ccggcgcacg
     1261 gcgcccagtc gcggcaatcg gggaacgttg gcttcggcgc ccgcgtcggt cggcacgatg
     1321 acctcctgcg cggcagcgac ctgcgcaccg tcggcggcga ccacgaggtc ggctgcgggg
     1381 tcgacgttgc gtttgatcac ggcgagcgcg atcgggccga gctcgtggtg gatcgcggcc
     1441 gaggtgatgc gtccgacctc ggtgggcgcg gagtcatccg cgctgggcag cagcaccggg
     1501 tcgcctggcc cgggcagcac gccctccgac ccgtcgaggt gcagcatcac gagccgccgc
     1561 ggcgggtggc cgaggttgtg caccttggcg acagtctcct gaccgcggta gcagcccttg
     1621 ttgaggtgca cggccgagcg cagccagtcc agctcgtgcg gaatcgtctt ctcgtcaacc
     1681 tcggtcgaca accgcggacg ccacgcggcg atccgcagag cctccagcgc gagagtgcca
     1741 gccgcgggtg agtcggcgag ggatgccgcg gcggcagcat ccgcgcggtc cacgagcacc
     1801 tcggcatacg tccaccccgc ggcggggtgc tgcgcggggt gcgcgtactg gtggccgccg
     1861 ggggcgacat ccagccacgg gtcgcgccag atcaggggga tgccgttggg ggcggcggcg
     1921 gggatctgcg ccggcgaggt ctcgaccagc tcgaccgacg gggaggtctc gaccagctcg
     1981 accaccggcg cgagcgtgcc gaacgtcgcg tagtcggccg agcgatcggc gacctccacg
     2041 cgcagcatga agcgcatgcg gtcgagccag gcggccagtg gcgcaccctc ggcaccgtct
     2101 accagcagcc acgcggtgac gccgtcgtcg atgacgcgga tggcgtgctc gagacgtccg
     2161 gtcgggtcga gaagcagggt ctcggccgac gcccccgccg cgaggccggc cagggactgc
     2221 gacgtcaggg agtcgagcca ggtgaggcga tcgggtccgg tgacggtgat gaccgcccgg
     2281 tcggacaggt cgacgatcgc ggagcccgcg gcgagcgcgc gctgctcgcc gagcgggttg
     2341 ccgtagtgct ggggcgggga gccgacggcg ttggggcggc gggcgaaggg cgcgggggtc
     2401 tcgacaggct cgaccaccga ggcggggggc accggctcag tcgacacgag cgagcctgcc
     2461 cgacgcgtgg gtcttcagct gctggccgag ggcggcgatg tcccacgccc acagcaggtg
     2521 cgagtcgacc aggccgtaca agcgggtggc cgcggcgtac tccttggcgc tggcgctgcg
     2581 caccacggca tccgtggcca ggtcgatgcg ggcgcccttg acctgaccga ggtacagctc
     2641 gctgacgccg ttgggatgca cgatcgccgc ctcgatatcg aagccgccgt cggagttgcg
     2701 cagggtctca accgccgagg cggtggtgaa gggcttgtca cccgtgccgg cgagcatgcc
     2761 cggccccggg tcgccctccg cggcggggcg gctgagccgc cagtaacccg tctcggtgac
     2821 gtgcggggtg agcgcgtcat ccagaagcca ggcgtaggag ttgtagttga ggtgcggcag
     2881 gccgtcgtgg gtgaagctga cccggtgccc gaactcgagc gtcttggtct cgtcgccgac
     2941 gccgtagtgc acgaccccgg tgccctccca gacgccgagc agccacgaga gtggcgcgag
     3001 ttcggccgga atatcggcgt tgagcgagat catggtcgcc tcaggcgccc agcgactagc
     3061 gctggccgcg gtacaggttg taaagcacga cgaccgaaac gaacccgatc gagagcgtgg
     3121 ccagcagcaa caggccgacg aagaagagct caagggcgag aagcatgagt caatactatc
     3181 gggtggtttc gatacgcggc ctgcggtcgc tactcaaccc aagggtttcg atacgcggcc
     3241 tgcggtcgct actcaacccg agggtttcga tacgcggcct gcggtcgcta ctcaacccaa
     3301 gggtttcgat acgcggcctg cggtcgctac tcaacccacc gggtttcgat gcgcggcccg
     3361 cgcgctagcc gatcagagcg aacacggccg aggcaatggc cagcacgacg atcgcgccgg
     3421 tgacactcgc catggcgcga accacgaagc cctctttgcg ctgaatcgcc agctggatga
     3481 agaacgtcac gatgacggca ccggcgagcg ccaccccgag ccaggtcacg cgtgcctcgg
     3541 gtgcggacgt cagcccgatg atcactgccg cggccgccgc cgccagccac acgggcacca
     3601 cgctccagat ctgccacgtc atacagtgat cctagagtgg gtcgtagact gtcccctacg
     3661 cacttggagg tcgattggcg cagctgttga tccttacctc tgcggtcgac agtgaagtct
     3721 tccccgcatt ggggctcctg agccacacca cgaagcagat acccgcctca cctgcttcgc
     3781 tcatctccgc gcccacctgc gacctcatat ttgtggatgc tcgccaggat ctcgccagcg
     3841 cgaagtccct gtgcaagatc ctcaccacga cgggcgtgac cgttccgctc atcctcatcc
     3901 tcaccgaggg cggcctgacc gcagtctcgg ccgactgggg tgtcgatgac gtcatcctcg
     3961 cgactgccgg tcccgccgaa gcggatgccc gcatccggct cgctctcggc cgccaggcac
     4021 tcgactcctc cagctccaag atccaggcct cgggtgtcgt catcgacgag gccagctact
     4081 ccgcgaaggt gcacggccga ccgctcgatc tcacgttcaa ggagttcgag ctgctgcgct
     4141 tcctcgcgac gcacccgtcg cgggtgttca cgcgcgagca gctgctcagc gaggtctggg
     4201 gctacgacta cttcggcggc acccgcacgg tcgacgtgca cgtgcggcgc ctgcgtgcca
     4261 agctcggcga cctcgagtcg ctcatcggca ccgtgcgcaa tgtgggctac cgcttcaacg
     4321 tctacgacga cgacgccgag cgactgacgc cggctcccgt agcctcgagc tgagagcgct
     4381 cccgcagtgg ggtcgggtgg tgggagcgac gcgggcnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnncggc tcgcgcggct gatcgcccgc gctcgggcag cggacgggca
     4561 tccgccgttc tcagacggag cgctggccga actcgcgacc ggcgcccgcg aggtcgtgtg
     4621 gctcgatgac gtcgctgctg cggtgcgcac ggtcactgac gcgtcgagcg cctcggtgac
     4681 cgaggccgag ttcgtcgtcg acccggatgc ccggcgccac ggtcacggac gcgccatgct
     4741 ccaccgcgtg ctcgtcgacg ccccgggtga cctgctggtc tgggcccacg gcggccaccc
     4801 cggcgcgcgc cgcctcgcgg ccagccacgg actcgagccg gttcgggagc tgctgcatct
     4861 gcgcggggag gtcccctccg gcgccgagtt cgcttggaag acccatgcgg cgggcgaacc
     4921 attggccacc ttggcgaact cggtgcacgg cgccgtggat gccctgctcg ccgtcaacgc
     4981 gcgggcgttc gccgagcatc cggagcaggg gcggctcacg cgtgccgagt tcgacgccct
     5041 cgccgccgag ccgtggttcg accccgagga tgtgctgctg gcgtgggacg gggaccggct
     5101 cgccgggttc tgctggttga aggtggaggg cggcggcagc ggcgagttct acgtggtcgg
     5161 ggtcgatccg gactaccagg ggcagggatg gggacgacgg ctggtcgagg cgggcatggc
     5221 gcggttggcg agccggggca tccgcgaggc cgatctctac gtcgaagccg acaacgcacc
     5281 agcgcttcgg ctgtaccggg agttcgggtt ccgcgaggac agcatcgaca tccagtaccg
     5341 ctggcgcagc gaccccggcc actgacaccg acttaacccc cagttcaccc ggcagtggca
     5401 ggatgaacgg atggacagtg accagagcct cgccgacatc ggggaggcga ccgactccct
     5461 cgacgagtac gacctcgacg agtcgtcgag tgtcgacgac ggctccctcg cgcccgaacg
     5521 ctacctcgac cgcgagctga gttggctcgc cttcaaccag cgcgtgctcg acctcgccga
     5581 agacgagatg ctgcccctgc tggagcgcac caactt
//
LOCUS       DAOSOU010000139         5608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14591_length_5608_cov_6.563839, whole genome shotgun sequence.
ACCESSION   DAOSOU010000139 DAOSOU010000000
VERSION     DAOSOU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14591_length_5608_cov_6.563839"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLW15_08125"
     CDS             complement(<1..730)
                     /locus_tag="PLW15_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HOT34397.1"
                     /translation="MLANFLIGLREGLEAALVVGILVAYLRKIGRDDVLPRIWLGVGL
                     AVALSLAIGAALTFGTYGLSFQAQELIGGILSIVATGFVTWMVFWMLRAARGLSRELR
                     SDVDSHLAGAGWGLVLVAFLAVGREGIETALFIWAAVQASGSTALPLAGAALGIAVAV
                     VLGYLIYRGVLSINLSKFFTYTGVILIVVAAGVLAYGVHDLQEAGVLPGINSLAFDVT
                     AFVPPDSWYGTLLKGTLNFSPATTW"
     gene            835..1983
                     /locus_tag="PLW15_08130"
     CDS             835..1983
                     /locus_tag="PLW15_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT34398.1"
                     /translation="MTERVLFVHAHPDDESISTGATIATLIDRGASVTVLTLTRGERG
                     EVIPDELQHLVDAPEQLGSAREAELAEAMRILGVSDHRILGNPDARWQGREPRRYVDS
                     GMSWGKRGAQASGILDPDSLTAADAGEVAADIAAVLIDVEPDVVVSYAADGGYGHPDH
                     VRAHEATRTAAQVIGVPFYVIAPRGRAAVVVNDPAVAQRKAAALAAHRTQVLVQGDTF
                     SLSSGEPRPLGEPERFDRLRPARAFSSMSLPSRIGSSVLGLVIGAFAGSVLTVAHRAT
                     VDVLGVTVPWGIIATVVITAALLTGLSLVFESRVVAACAAVGLLGAILLLSLGSAGGS
                     VLVPDDVVGYIFLIAPVVIAGVVLAWPQDGRWRRGKMGIPAAEGPDQK"
     gene            1980..2306
                     /locus_tag="PLW15_08135"
     CDS             1980..2306
                     /locus_tag="PLW15_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOT34399.1"
                     /translation="MTYVIAQPCVDLKDRACIDECPVDCIYEGERMLYIQPDECVDCG
                     ACEPVCPVEAIYYEDDLPGKWADYYKANVEFFDDLGSPGGASKVGVLKKDHPLVAALP
                     AGGGEG"
     gene            2307..3413
                     /gene="dapC"
                     /locus_tag="PLW15_08140"
     CDS             2307..3413
                     /gene="dapC"
                     /locus_tag="PLW15_08140"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HOT34400.1"
                     /translation="MARTPLKLPDFPWDSLAPFAERARAHPDGFIDLSVGSPVDPTPE
                     PIRHALAAATDAHAYPQTSGSPALREAIVDWFARRRGVDDLSVANVLPTIGSKELVAL
                     MPLMLGLGRGDVVVHPTVAYPTYAIGAALVGATAVASDDPADWPENTRLIWLNSPGNP
                     DGRVLDVDELKAAVARARELGAVVASDECYAELNWQGAEPTPSILDPRVIAGQRNSVL
                     AVYSLSKQSNLAGYRAAFVAGCSDLIDELLAVRKHAGLIPPAPVQAAMIAALADETHV
                     AEQKERYRARRATLAPALEAAGFRIDESAAGLYLWATKGEDSWATVAEFAELGILVVP
                     GSFYGEQPPRHVRIALTAPDAAIADAAARLELYV"
     gene            3492..4787
                     /locus_tag="PLW15_08145"
     CDS             3492..4787
                     /locus_tag="PLW15_08145"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOT34401.1"
                     /translation="MTDAAPESEKATLHFPGGSAEFPVIRGTDGHDSIDISTFTKQTG
                     FTTLDQGFVNTASTRSAITYIDGDKGILRYRGYAIEDVAKNSTYLEVAWLLIYGELPS
                     ESELAEFDEKIRRHTLLHEDLRRFFDALPHSAHPMSVLSSAVSALSTYYEDSLNVHDP
                     EQVEISTIRLLAKLPVIAAYAHKKALGQAFLYPDNSLSFVDNFLKLNFGTMAEPYVVN
                     PVLSTALDRLLILHEDHEQNASTSTVRLVGSTEANIFASISAGINALYGPLHGGANEA
                     VLKMLSEIKASGEGVEKFVERVKNKEDGIRLMGFGHRVYKSFDPRAKLVKESADEVLA
                     ALGVKDDLLDIAKELEAVALSDDYFIERKLYPNVDFYTGVIYKAMGFPTRMFTVLFAI
                     GRLPGWIANWREMNEDTYTKIGRPQQLYTGEPVREWPQR"
     gene            4800..5387
                     /locus_tag="PLW15_08150"
     CDS             4800..5387
                     /locus_tag="PLW15_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34402.1"
                     /translation="MKWFPRRRRFTLGTYVAPAQREATIEEMVDDGVLIAAAAVRLAV
                     KNLIILRSARDRADYSPERVMAAVREELENLATEKDADAARLREVGRRAAERDGQPRH
                     HDDYRSGDTELLAKRAEVSERLATRLRELGSDAEFCGELVDRARREAWEEIAASITAR
                     ARQSSRGHDPDYDRERGGRMLALLGDLADLENRER"
     gene            complement(5384..>5608)
                     /locus_tag="PLW15_08155"
     CDS             complement(5384..>5608)
                     /locus_tag="PLW15_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34403.1"
                     /translation="PRTRLACSVVDVSGRLSDLPGLVTGVPTNQWVTVAATDVFELPD
                     LTMGIRCYPDRDADLTVVVRDVVLAAEPTG"
BASE COUNT          937 a         1891 c         1908 g          872 t
ORIGIN      
        1 gccaggtggt cgcgggggag aagttgagcg tgcccttgag cagcgttccg taccaactgt
       61 ccggcggcac gaatgccgtc acgtcgaacg ccagcgaatt gatacccgga agcacccccg
      121 cctcctgcag gtcgtgcacg ccgtaggcga gcacccccgc ggcgacaacg atgaggatga
      181 cgccggtgta cgtgaagaac ttgctgaggt tgatcgacag cacgccgcgg tagatgaggt
      241 acccaagcac gaccgcgacg gcgatcccga gagcggcacc ggccaggggc agggcggttg
      301 agccgctcgc ctgcacggcg gcccagatga acagcgccgt ctcgataccc tcccggccca
      361 cggcgaggaa agcgaccagc acgagtcccc agccggcgcc ggcgaggtgg gagtcgacgt
      421 cgctgcgcaa ctcgcgcgac aggccgcggg ccgcgcggag catccagaac accatccagg
      481 tgacgaagcc ggtggcgacg atcgacagga tgccgccgat gagctcctgc gcctggaacg
      541 acaggccgta ggtaccgaag gtgagggccg caccgatggc gagggagagt gcgacagcga
      601 ggccgacgcc cagccagatg cgggggagca cgtcgtcgcg gccgatcttg cgcaggtagg
      661 cgacgagaat accgaccacg agcgcggcct cgaggccctc gcggaggccg atcaggaagt
      721 tggcgagcac gtgagctttc gtgagatctg gggttggtta ggctaacctt acctatcgac
      781 cctagcccgg aggacgtatc ctgtctaccc ggcgagaaat tgaggggtag tcaggtgacc
      841 gagcgagtgc tctttgttca cgcgcatccc gacgacgagt ccatatcgac cggtgcgacg
      901 atcgccacgc tgatcgatcg cggcgcctca gtgacagtgc tcaccctcac gcggggtgaa
      961 cgcggcgagg tgatccccga cgagctccag catctggtcg acgcgcccga gcagctcggc
     1021 agcgcgcgcg aggccgaact cgccgaagcg atgcggattc tcggggtcag tgaccaccgc
     1081 atcctgggta accccgacgc tcgttggcag gggcgtgagc cgcggcggta cgtcgactcc
     1141 gggatgagct ggggcaagcg gggtgcgcag gccagcggca tcctcgaccc cgattcgctc
     1201 acggcggcgg acgcgggcga ggtggccgca gacattgctg ccgtgctcat cgacgtcgaa
     1261 cccgatgtgg tggtgagcta cgccgccgac ggcggctacg ggcatcccga ccatgtgcgc
     1321 gcccacgagg cgacccgcac ggcggctcag gtgatcggcg ttccgttcta cgtgatcgcc
     1381 cccaggggca gggcggcggt ggtcgtcaac gatcccgcag ttgctcagcg caaggctgca
     1441 gcactggcgg cgcaccgcac acaggtgctg gtgcagggcg acaccttctc gctctcctcg
     1501 ggcgagccgc ggccgctggg cgaacccgag cgcttcgacc ggctccgccc ggctcgggca
     1561 ttctcgtcga tgagcctgcc gtcgcggatc gggtcgagcg ttctcggcct ggtgatcggt
     1621 gccttcgcgg gaagtgtgct cacggtcgcg caccgggcaa cggttgacgt actcggagtc
     1681 acggtgccgt ggggcatcat cgcgacggtg gtgatcacgg cggcgctgct gacaggactc
     1741 agtctggtgt tcgagagcag ggttgttgcg gcctgcgcgg ccgtcggtct gctcggcgcg
     1801 atcctgctgc tctcgctcgg ttcggccggt ggttctgtgc tggtgcccga cgacgtcgtc
     1861 ggctacatct tcctcatcgc tccggtcgtc atcgctggcg tggtgctggc gtggccgcaa
     1921 gacgggcgct ggcgtcgcgg taagatggga atccctgccg cggaaggtcc tgatcagaag
     1981 tgacgtacgt catcgcccag ccctgtgtcg atctcaagga tcgcgcgtgc atcgacgaat
     2041 gcccggtcga ctgcatctac gagggcgagc gcatgctcta catccagccc gacgagtgtg
     2101 tcgactgcgg agcgtgcgag ccggtctgcc cagttgaggc gatctactac gaagacgatc
     2161 ttccgggtaa gtgggccgac tactacaagg ccaacgtgga gttcttcgac gacctcggtt
     2221 cgcccggcgg ggcatccaag gtcggcgtac tgaagaagga ccacccgctt gtcgcggctc
     2281 ttcccgcggg cggcggcgag ggctgagtgg ctcgcacgcc gctgaagctt cccgacttcc
     2341 cgtgggattc gctggccccg ttcgctgagc gtgcgcgggc ccaccccgac gggttcatcg
     2401 acctctccgt aggctcgccc gttgacccga cccccgagcc gatccgtcat gcgctcgcgg
     2461 cggccacgga tgcccacgcc tacccccaga cgtcggggag ccccgcgctg cgcgaagcga
     2521 tcgtcgactg gttcgcgcgc cggcggggag tcgacgacct ctccgtcgcg aacgtgctgc
     2581 ccacgatcgg ctcgaaggaa ctcgtcgccc tgatgcccct gatgctgggg ctcgggcggg
     2641 gcgacgtcgt ggtgcacccc accgtggcgt acccgacgta cgcgatcggt gccgccctcg
     2701 tcggtgcgac ggccgtggca tccgatgacc ctgccgactg gccggagaac acccggctga
     2761 tctggctcaa cagcccgggc aaccccgacg gacgggtgct cgacgtcgac gagctgaagg
     2821 ccgctgtcgc gcgcgcccgc gagctcggag ctgtcgtcgc cagcgacgaa tgctacgccg
     2881 agctcaactg gcagggcgcc gagccgaccc cctcgatcct tgacccgcgc gtgatcgcgg
     2941 ggcagcgcaa ctcggtgctg gccgtctact cgctgagcaa gcagtcgaac ctcgccgggt
     3001 accgagccgc cttcgtggcc ggatgcagcg acctcatcga tgagctcctc gcggtgcgca
     3061 agcacgcggg gctgatcccg ccggcgccgg tgcaggcggc gatgatcgcg gccctggctg
     3121 acgagaccca cgtggcagag cagaaggagc gctaccgagc ccgtcgcgcg accctggcac
     3181 ccgcactcga ggccgccggc ttccgcatcg acgagagcgc ggccggactg tatctctggg
     3241 cgacgaaggg cgaggactcc tgggcgaccg tcgccgagtt cgccgagctc ggcatcctcg
     3301 tcgtgccggg ttccttctac ggcgaacagc cgccccggca tgtgcgtatc gccctgacgg
     3361 ctccggatgc cgcgatcgcc gacgccgcgg cccgcctcga gctgtacgtc tgactgtcgg
     3421 tttcctccaa caggttttca ccgcgtttag cactaacgac agtggcgagc gcgtgcttat
     3481 aagctgtaag cgtgactgac gccgctcccg aatccgagaa ggccaccctc catttccccg
     3541 ggggctcggc cgagttcccg gtgatccggg gcaccgatgg ccacgacagc atcgacatct
     3601 ccaccttcac gaagcagacc ggcttcacga ccctcgacca gggcttcgtc aacaccgcgt
     3661 cgacgcgaag tgcgatcacc tacatcgacg gtgacaaggg gattctgcgc taccgcggct
     3721 atgcgatcga ggatgttgcg aagaactcga cgtacctcga ggtggcgtgg ctgctcatct
     3781 acggcgaact gcccagcgag agcgagcttg ccgagttcga cgagaagatc cgccgtcaca
     3841 cactgctcca cgaagacctc cgacggttct tcgatgcgct cccgcacagc gcccacccca
     3901 tgtcggtgct gtcgagcgcc gtgtcggccc tctcgacgta ctacgaggat tccctcaacg
     3961 tgcacgaccc ggagcaggtc gagatctcca cgatccgcct gctcgccaag ctgccggtca
     4021 tcgccgccta tgcgcacaag aaggcgctcg gccaggcgtt cctgtacccg gacaactcgc
     4081 tgagcttcgt cgacaacttc ctcaagctca acttcggcac catggccgag ccctacgtcg
     4141 tcaacccggt gctgtcgacc gcgctcgacc gcctgctcat cctgcacgag gaccacgagc
     4201 agaacgcgtc cacttcgacg gtgcgactgg tcggctcgac cgaggccaac atcttcgcct
     4261 ccatctccgc gggcatcaac gctctctacg gaccgctgca cggcggcgcc aacgaggccg
     4321 ttctcaagat gctgagcgag atcaaggcgt cgggcgaggg cgtcgagaag ttcgtggagc
     4381 gggtgaagaa caaggaagac ggcatccgtc tcatgggctt cgggcaccgc gtctacaaga
     4441 gcttcgaccc gcgcgccaag ctggtcaagg agagcgcgga cgaggtgctc gcagcactcg
     4501 gcgtcaagga cgacctgctc gacatcgcca aggagctcga ggccgtggcg ctcagcgacg
     4561 actacttcat cgagcgcaag ctctacccca acgtcgactt ctacaccggg gtcatctaca
     4621 aggcgatggg cttcccgacc cgcatgttca cggtgctgtt cgcgatcggc cgcctgccgg
     4681 gctggatcgc caactggcgc gagatgaacg aagacacgta caccaagatc ggccgccccc
     4741 agcagctcta cacgggcgag cccgtgcggg agtggccgca gcgctagtgg tggtgtccgg
     4801 tgaagtggtt ccctcgacgt aggcgtttca ctctcggaac ctacgtcgct cccgctcagc
     4861 gggaggcgac gatcgaggag atggtcgacg acggcgttct gatcgccgcc gctgctgttc
     4921 gccttgcggt caagaacctg atcatcctgc ggtccgcgcg ggaccgagcc gactactcgc
     4981 ccgagcgggt gatggcagcg gttcgcgagg aactcgagaa cctcgccacg gagaaggacg
     5041 ccgacgcggc tcgcctgcgc gaggtgggcc gtcgtgcagc ggagcgggat gggcaacccc
     5101 gtcaccacga cgactatcgc tcgggtgaca ccgaacttct cgccaagcgt gccgaggtct
     5161 cggagcggct ggcgacgcgc ctgcgcgagc tcggaagtga tgccgagttc tgcggcgagc
     5221 tggtggatcg ggcccggcgc gaggcctggg aggagatcgc ggcatccatc acggcgcggg
     5281 cacgccagtc gtctcgaggc cacgaccccg actacgaccg ggagaggggc ggccggatgc
     5341 tggcgctgct cggtgacctc gccgacctcg agaaccgcga gcgctagccg gtcggctccg
     5401 cggccagcac cacgtcgcgc accaccaccg tgaggtcggc gtcgcggtcg ggatagcagc
     5461 ggatgcccat ggtgaggtcg gggagctcga agacatcggt cgctgcgact gtcacccact
     5521 ggttggtggg aacgcccgtc accagccccg gcagatcgga cagccgtccg ctgacgtcga
     5581 cgacgctgca ggcgagccgg gtgcgggg
//
LOCUS       DAOSOU010000140         5607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14597_length_5607_cov_4.645173, whole genome shotgun sequence.
ACCESSION   DAOSOU010000140 DAOSOU010000000
VERSION     DAOSOU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14597_length_5607_cov_4.645173"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLW15_08160"
     CDS             <1..765
                     /locus_tag="PLW15_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOT34404.1"
                     /translation="VVSQRFDHEISAEPLDVYRVLRTLNPSPYMYLLTLVDPAGEPYS
                     IVGSSPEALVKVSQSRVYMHPIAGSRPRGETPERDLDLAEELLADDKEKSEHLMLVDL
                     ARNDLLKVCTPGTVEVTEFMQVERFSHIMHLVSSVEGDLRADASAVDVFRATFPAGTL
                     SGAPKPRALEIIDELEPAQRGVYGGVVGYLDFAGDADLAIAIRTATIVGGLARVQAGA
                     GLVADSDPAAEHLESQNKAAAPLRAVAVANAMKRVP"
     gene            762..1328
                     /locus_tag="PLW15_08165"
     CDS             762..1328
                     /locus_tag="PLW15_08165"
                     /inference="COORDINATES: protein motif:HMM:NF021079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HOT34405.1"
                     /translation="MNGRRLKLGAILAGLVLSGLTLLAWTQQWFSIELIPDGSVAVGG
                     DVAAPALAALALSGLALVAALAIAGPVFRVILGLLEAAIGTLVVVSAVLAIVDPVAAS
                     ASAITAVTGVSGTASVIALVSTVAPTAWPFVALLAGALVVAVGITIVVSGRFWPQSSR
                     KYQTARLAEVDGSPIGDWDALSDGRDPT"
     gene            1373..1603
                     /locus_tag="PLW15_08170"
     CDS             1373..1603
                     /locus_tag="PLW15_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HOT34406.1"
                     /translation="MNTQSDPGHGHSPAAWAAVIIMLIAIALGTLAFWLDMAWLVWAS
                     VALLVVGALVGWILAKVGYGVAGPKYVPKAHN"
     gene            1604..2377
                     /gene="trpC"
                     /locus_tag="PLW15_08175"
     CDS             1604..2377
                     /gene="trpC"
                     /locus_tag="PLW15_08175"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOT34407.1"
                     /translation="MLDELVAGALHDASARRAARSLAEVEAAALARPPALDVLSALAP
                     RDRVHIIAEIKRSSPSKGALAEIADPASLAVAYENGGASAISVLTEGRRFGGSLEDLE
                     AVRAVASVPVLRKDFIAEPYQVLEARAAGADLVLLIVAALDQDTLSSLFRLINGLGMT
                     PLVETHSADELDRALDLGADLVGVNARDLSTFELDQDLFGRLADMIPSGVIRVAESAV
                     KSPADVAHYRRGGADVVLVGEALVTGADPDETLREFLAV"
     gene            2377..3585
                     /gene="trpB"
                     /locus_tag="PLW15_08180"
     CDS             2377..3585
                     /gene="trpB"
                     /locus_tag="PLW15_08180"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT34408.1"
                     /translation="MALRDELGPYFGEYGGRYVPESLIAALDELDSAYQLARTDPAFQ
                     AELAHLHATYTGRPSIITEVPRFAQHAGNARVILKREDLNHTGSHKINNVLGQALLAK
                     RLGKRRLIAETGAGQHGVATATAAALFGMECVVYMGEVDTERQALNVARMRLLGATVI
                     PVTTGSRTLKDAINDALRDWVANVDSTHYLLGTVAGPHPFPVMVRDFHKIIGEEARAQ
                     VLELTGRLPDAVTACVGGGSNAMGIFHAFLDDPDVALYGYEAAGEGHLTERHAATITR
                     GRPGVLHGARSYMLQDDDGQTIESHSISAGLDYPGVGPEHAWLNDIGRASYLPITDDE
                     AMEALRLLSRTEGIIPAIESAHALAGTMKLGRELGPDATILVNLSGRGDKDMETAGTY
                     FELFDKKASE"
     gene            3582..4394
                     /gene="trpA"
                     /locus_tag="PLW15_08185"
     CDS             3582..4394
                     /gene="trpA"
                     /locus_tag="PLW15_08185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOT34409.1"
                     /translation="MSTAGTSTVETVIAERKAAGSGALVGYLPVGFPDFQTSVDAAVA
                     LAENGVDVIELGLPYSDPVMDGTVIQKATQAALAGGFKLAHGFDAVAAITARTDVPVL
                     VMTYWNPVLQYGVDRFSEDLVAAGGAGLITPDLVADEAGLWLAASERTGLDRVFLAAP
                     SSTDARLAQAVELSRGFVYAVSTMGITGARSDVDTAARTLVARLRSAGATSTCVGLGI
                     STADQVREVLGYADGAIVGSALVTALADGGVEAVARTAAALAAGTRLTSSST"
     gene            4477..5493
                     /gene="lgt"
                     /locus_tag="PLW15_08190"
     CDS             4477..5493
                     /gene="lgt"
                     /locus_tag="PLW15_08190"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT34410.1"
                     /translation="MSSTFGWALPFTFRIHTYAVLILIGIILATIWVNSRLKARGAES
                     GIVLDVIIWAVPLGLVGARAWHVATHLGDYFYDGSNPWNPTAPGSVWAIWEGGNAIFG
                     ALVGGAVGAYIGCRIVGLRFWTFADALAPAMLLAQATGRLGNYFNHELFGQPTDLPWG
                     LEIEATNPAFPVGLPSGTLFHPTFLYEMIWNVVGIVFLLTMERQFRLVRKTVFGLSLP
                     VPVGDAQPRLQWGKVWALYMIWYGIGRFYFEGIRVDPSDVFFGLRSNQWGAVLAVVLG
                     IILFLVQRRNHPGVEPSAYMPGREPAGAVDSEATYSDTDEPGDDAPEVTGKHATSGAG
                     TTSS"
BASE COUNT          848 a         1962 c         1812 g          985 t
ORIGIN      
        1 gtcgtctcgc agcggttcga ccacgagatc tccgccgagc cgctcgatgt ctaccgggtg
       61 ctgcgcaccc tcaacccgtc gccgtacatg tacctgctca cgctcgtcga cccggccggt
      121 gaaccgtact cgatcgtggg ctcgagcccc gaggcgctcg tcaaggtgtc gcagtcgcgg
      181 gtgtacatgc atccgatcgc cggctctcgc ccgcgcggcg agacgcctga gcgcgacctc
      241 gacctcgccg aggagctcct cgccgacgac aaggagaagt ccgagcacct catgctcgtc
      301 gacctcgctc gcaacgacct gctcaaggtc tgcacccccg gcactgtcga ggtgaccgag
      361 ttcatgcagg tcgaacgctt cagccacatc atgcacttgg tctcctcggt ggagggtgac
      421 ctgcgagcgg atgcgagcgc ggtggatgtc ttccgcgcaa ccttcccggc cggaactctc
      481 tctggcgcgc ccaagccgcg ggcgctcgag atcatcgacg agctggaacc cgcgcagcgc
      541 ggcgtgtacg ggggagtggt cggctatctc gactttgcgg gcgacgccga cctcgccatc
      601 gcgatcagaa cggccaccat cgtgggcggc ctcgctcggg ttcaggcggg agcgggtctg
      661 gttgccgatt ccgatcctgc tgccgagcac ctcgagtcgc agaacaaggc ggcggcaccg
      721 ctgcgcgcgg tggccgtcgc gaacgccatg aagcgggttc cgtgaacggc cgacggctga
      781 agctcggcgc gattctcgcg ggtctcgtgc tgagtgggct gaccctcctg gcgtggacgc
      841 agcagtggtt cagcatcgag ctcatccccg acggctcggt tgctgtcggc ggggatgtcg
      901 cagccccggc actggccgcg ctcgcgctct ccggcctggc actcgtcgcc gcccttgcca
      961 tcgccggccc ggtcttccgg gtcattctcg gtctgctgga ggcggcgatc ggcacactcg
     1021 tcgtcgtctc ggctgttctc gcgattgtcg atcccgttgc cgctagcgcg tcggcgatca
     1081 cggcggtcac tggcgtgagc gggacagcat cagtcattgc tctcgtcagt acagtggccc
     1141 cgacggcatg gcccttcgtg gcgctgctcg cgggcgccct ggtcgtcgcc gtgggcatca
     1201 cgatcgtcgt ctcgggtcgt ttctggccgc aatcgtcacg caagtaccag acagcgcgtc
     1261 tggctgaggt cgacgggtct ccgatcggcg actgggacgc tctcagtgac gggcgcgatc
     1321 ccacctgacc gctagaattg ccatgacgac gtcacagaat ggagcaccga cgatgaacac
     1381 ccagtctgac cccggccacg gacactctcc ggccgcatgg gccgccgtga tcatcatgct
     1441 cattgcgatc gccctgggca ccctggcttt ctggctggac atggcgtggc tcgtctgggc
     1501 ctctgtggca ctgctcgtgg tcggcgctct cgtgggctgg atcctcgcga aggtcggcta
     1561 cggagtagct gggcccaagt atgtgccgaa ggcgcacaac taggtgctcg acgaactcgt
     1621 agccggagct ctccacgacg cttcggcccg tcgtgctgcc cgcagtcttg ccgaggtcga
     1681 ggcggcagcc ttggcgcgtc caccggcgct cgatgtgctc tccgccctcg caccgcgcga
     1741 tcgcgtgcac atcatcgcgg agatcaagcg ttccagtccg tccaagggcg ccctcgcgga
     1801 gatcgccgac ccggcatccc ttgctgtcgc ctacgagaac ggcggagcga gtgccatcag
     1861 cgtcctgact gagggtcgac gtttcggcgg gtcgctggag gacctcgaag cggtgcgggc
     1921 cgtggcatcc gtgccggtgc ttcgcaagga cttcatcgcc gagccctatc aggtgctcga
     1981 ggcccgtgcc gccggagcag atctcgtgct cctgatcgtc gccgcgcttg accaggacac
     2041 cctgtcgtcg ctgttccggc tgatcaacgg tctgggtatg acacccctgg tcgagaccca
     2101 ctcggccgac gaactcgatc gtgcgctgga tctcggcgcc gatctggtcg gcgtcaacgc
     2161 gcgcgacctc agcactttcg aactcgacca ggacctcttc ggacgcctgg cagacatgat
     2221 cccgagcgga gtgatccggg tggccgagtc tgccgtcaag tcccctgccg atgtggcgca
     2281 ctatcgccgc ggcggcgccg atgtcgttct cgtgggggag gcgctcgtca cgggcgccga
     2341 ccccgatgag accctgcgcg aattcctggc cgtctgatgg cacttcgcga tgagctcggc
     2401 ccgtacttcg gtgagtacgg tggccgctat gtccccgagt cactgatcgc cgcgctcgac
     2461 gagctggatt ccgcgtatca actcgcccgc accgatcccg cgttccaggc cgagctcgcc
     2521 cacctgcatg cgacctacac ggggcgcccc agcatcatca ccgaggttcc gcggttcgcg
     2581 cagcacgcgg gtaacgcccg cgtcatcctg aagcgtgagg atctcaacca caccggctcg
     2641 cacaagatca acaacgtgct cggtcaggcg ctgctcgcga agcgtctcgg caagcgtcgc
     2701 ctgatcgccg agaccggggc aggccagcac ggtgtcgcaa cggcaacggc agccgcgctg
     2761 ttcggcatgg agtgcgtcgt ctacatgggc gaggtcgaca ccgagcgtca ggcgctcaac
     2821 gtcgcccgca tgcgccttct cggtgccacc gtcatccccg tcaccaccgg ctcgcgcacc
     2881 ctgaaggacg cgatcaacga cgccctgcgc gactgggtgg ccaacgtcga ctccacgcac
     2941 tatctgctcg ggacggtggc tggcccgcac ccgttccccg tcatggtgcg cgacttccac
     3001 aagatcatcg gcgaggaggc ccgagcccag gtgctcgagc tcaccggacg cctgcccgac
     3061 gcggtcacag cctgcgtcgg cgggggcagc aacgcgatgg gcatcttcca cgccttcctc
     3121 gatgacccgg atgtcgcgct ctacggctat gaggcggcgg gcgagggtca cctcaccgag
     3181 cgtcacgccg caaccatcac gcggggtcgc cccggcgttc tccacggagc ccgcagctac
     3241 atgctgcagg acgacgatgg gcagacgatc gagtcgcact cgatctccgc ggggctcgac
     3301 taccccggcg tcggtcccga gcacgcgtgg ctcaatgaca tcggtcgcgc gagctacctg
     3361 ccgatcaccg acgacgaggc catggaggct ctgcgcctgc tcagccgcac cgagggaatc
     3421 atcccggcaa tcgagtcggc tcacgccctc gccggcacga tgaagctcgg tcgcgagctc
     3481 ggccctgacg cgaccatcct cgtcaacctc agcggccgcg gcgacaagga catggagacg
     3541 gcgggcacct acttcgagct gttcgacaag aaggccagcg aatgagcacc gccggaacga
     3601 gcacggtcga gacggtcatc gccgagcgca aggctgccgg ttcgggcgct ctcgtgggct
     3661 acctccccgt cggcttcccg gacttccaga cgagcgtcga cgcagcagtg gccctggcag
     3721 agaacggtgt cgacgtcatc gagctgggtc tgccctactc cgaccccgtc atggacggca
     3781 cggtcatcca gaaggcgacc caggccgcct tggcgggcgg gttcaagctc gcgcacgggt
     3841 tcgatgctgt tgcggcgatc accgcgcgca ccgacgtgcc agtgctcgtc atgacgtact
     3901 ggaaccccgt gcttcagtac ggtgtcgatc gcttctcaga agatctcgtc gctgctggcg
     3961 gggcgggact catcaccccg gacctggttg ccgacgaagc cggactctgg ctcgcggcat
     4021 ccgagcgcac gggtcttgat cgggtcttcc tcgcggcccc cagctcgacg gatgcccggc
     4081 tcgcccaggc cgtcgagctg agtcgtggct tcgtctacgc cgtgtccacc atgggcatca
     4141 ccggagcccg cagcgacgtg gacaccgcgg cgcgcacact cgtggcccga ctgcgctcgg
     4201 ccggcgccac cagcacctgc gtcgggctgg gtatctccac ggccgatcag gtacgcgagg
     4261 tgctcggata cgccgacggc gcgatcgtcg ggtcggccct ggtcactgct ctcgccgacg
     4321 gtggtgtcga ggccgtcgca cgtacagcgg cagcactggc cgccggtact agactcacgt
     4381 cgtcctccac ttagaaaggt gatcggcttg tcgctcctgc cgctcagcat ccccagcccg
     4441 cccgtcgaat ggcagtactt cgacctcacg gcgtggctga gttccacctt cgggtgggct
     4501 ctgccgttca ccttccgcat ccacacgtat gcggtgctca tcctcatcgg gatcatcctt
     4561 gcgacgatct gggtcaacag ccgactcaag gcgcgtggcg ccgaatcagg gatcgtgctc
     4621 gacgtgatca tctgggcggt tccactgggc ctggtgggag cgcgcgcctg gcacgtggcg
     4681 actcacctcg gcgactactt ctacgacggt tccaacccgt ggaacccgac ggctcccggc
     4741 agcgtgtggg ccatctggga gggtggcaac gcgatctttg gcgccctggt gggtggggcc
     4801 gtcggtgcgt acatcggatg ccgcatcgtg ggcctgcgct tctggacctt cgcggatgcg
     4861 ctggctccgg ccatgctcct cgcgcaggcg accggccgac tgggcaacta cttcaaccac
     4921 gagctgttcg gccagccgac ggatctgccg tggggtctcg agatcgaagc caccaacccg
     4981 gcgttcccgg tcgggctgcc gtcgggcacc ctcttccacc ccacgttcct gtacgagatg
     5041 atctggaacg tcgtgggtat cgtcttcctg ctcacgatgg agcgccagtt ccgtctggtg
     5101 cgcaagaccg tcttcggcct ctcgctgccc gtgccggttg gcgacgccca gccgcgtctg
     5161 cagtggggca aggtgtgggc cctgtacatg atctggtacg gcatcggacg cttctacttc
     5221 gagggcatcc gggtcgatcc tagcgacgtc ttcttcggtc tgcgctcgaa ccagtggggc
     5281 gccgtgctgg cggtggtgct aggaatcatc ctgttcctcg tgcagcgccg caaccacccc
     5341 ggcgtggagc cgagcgccta catgcccggc cgcgaaccgg cgggtgctgt agactccgaa
     5401 gcgacctatt cggacactga cgaacccggc gatgatgctc ccgaggtgac cggaaagcac
     5461 gccacaagcg gagcaggcac cacgtcctct taaaaccggg gccgcattcc ctttgggcca
     5521 tcgacgtccc gctccagcca gcaccaacgt gaggacggtt cccatggctc tcacgccacc
     5581 cttcgagcgg ttcagctcca cgcctgc
//
LOCUS       DAOSOU010000141         5595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14632_length_5595_cov_5.470036, whole genome shotgun sequence.
ACCESSION   DAOSOU010000141 DAOSOU010000000
VERSION     DAOSOU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14632_length_5595_cov_5.470036"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLW15_08195"
     CDS             complement(<1..791)
                     /locus_tag="PLW15_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOT34411.1"
                     /translation="MIGTTTRDDFDARIAGGHRVVPVIRELFADGETPVGIYRKLAQG
                     KPGTFLLESAEQGGIWSRFSFVGVSSFGVLTQSGENVVWLDYGLSEERALGAEHPSRP
                     LDALAHLYERWGTPRVDGHPPLTGGLVGFIGWEAIRQIEHLPDAPPADFSVPGQALGF
                     VSSLVVLDHRYGTVLLIAAALNDGVDDSSTLWSDAQARLDDMQVALSRPSEAWLAEVD
                     LTIAPTPIPRTTPDAYAAAIEKSKQYIRDGDVFQVVVSQRFDHEI"
     gene            complement(788..1159)
                     /gene="hisI"
                     /locus_tag="PLW15_08200"
     CDS             complement(788..1159)
                     /gene="hisI"
                     /locus_tag="PLW15_08200"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOT34412.1"
                     /translation="MSELTDATVGEVIDRAVFGDNGLIPAIVQEAGSKDVLMLGYMDA
                     EALRRTLTEGRVTFWSRSRQEYWRKGDTSGHVQYVRAAALDCDADALLVTVDQHGPAC
                     HTGAHSCFDVDPLSPTLGGPS"
     gene            complement(1156..1908)
                     /gene="hisF"
                     /locus_tag="PLW15_08205"
     CDS             complement(1156..1908)
                     /gene="hisF"
                     /locus_tag="PLW15_08205"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT34413.1"
                     /translation="MGVAVRVIPCLDVADGRVVKGVNFLNLRDAGDPVELARKYYEQG
                     ADEITFLDVTATVDNRATTYDVVRATAEQVFIPLTVGGGVRSAEDVARLLASGADKVG
                     VNSAAIARPELVGEIADRFGAQVLVLSLDVKRSGGRFVVTTHGGRTETDLDALDWARE
                     AIERGAGELLVNSIDADGTKRGFDLELVTAMRELSHVPVIASGGAGAVGDFAPAIAAG
                     ADAVLAASVFHNGELTVGDVKQALSDSGMVVR"
     gene            complement(1911..2750)
                     /gene="hisG"
                     /locus_tag="PLW15_08210"
     CDS             complement(1911..2750)
                     /gene="hisG"
                     /locus_tag="PLW15_08210"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOT34414.1"
                     /translation="MLRIAVPNKGSLSEIAAEMLAEAGYAGRRDLRALSALDPRNDVE
                     FFYLRPRDIATYVGSGALDVGITGRDLLLDSRSTAQEIAALDFGESTFRFAAAPGRFT
                     QLGDLAGVRVATSYPALVGDFLARNGVQVSIVSLDGAVESAVKLGVADAVADVVSTGS
                     TLRAQGLEIFGPVILDSTAVLISAKPDLAGIATLQRRLQGVLVARQYVLVDYDVPVAL
                     VETATRITPGLESPTLSPLHDPEWVAVRAMVLRSDANHVMDELYEVGARAILVSAIHA
                     ARI"
     gene            complement(2787..3050)
                     /locus_tag="PLW15_08215"
     CDS             complement(2787..3050)
                     /locus_tag="PLW15_08215"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOT34415.1"
                     /translation="MKTFDELFAELSDKAVTRPTGSGTVAELDAGVHAIGKKIVEEAA
                     EVWMAAEFQSDAETAEEISQLIYHLQVLMLAKGLTPADVWRHL"
     gene            complement(3091..3465)
                     /locus_tag="PLW15_08220"
     CDS             complement(3091..3465)
                     /locus_tag="PLW15_08220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT34416.1"
                     /translation="MTDSPRTKPEVDFYEGPEPTELVIIDLEEGTGEVAQPGATVDVH
                     YLGVDFETGEEFDSSWNRGQSIQFPLSGLIKGWQEGIPGMKVGGRRQLICPPALAYGP
                     AGGGHRLSGRTLTFVIDLLGVK"
     gene            3511..3954
                     /locus_tag="PLW15_08225"
     CDS             3511..3954
                     /locus_tag="PLW15_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOT34417.1"
                     /translation="MRAVALASSAREHGNDPFGALLVTESGVVVEAENTVVTASDPTG
                     HAETNLVRLAAAQLTPAELTTATLYTSTEPCAMCCGAIFWAGIGRVVYALAESELNAM
                     LPPESIGLVLDLPSREVFARGGHQTEVIGPIAVPGAAEVHAGYWS"
     gene            complement(3951..4613)
                     /gene="rpe"
                     /locus_tag="PLW15_08230"
     CDS             complement(3951..4613)
                     /gene="rpe"
                     /locus_tag="PLW15_08230"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT34418.1"
                     /translation="MGARINPSILAADFVNLQRDVASIASADFVHVDVMDNHFVPNLT
                     FGLQMVGRLQDVSPRPLDVHLMITDVDRWAPDYAKTGAASVTFHAEATEHPVELARTL
                     RSLGARAGIALKPGTAAEPLLDLLHEFDQVLIMTVEPGFGGQSFMVSTMPKLAQVREA
                     ITRAGTDVWLQVDGGVDLTTIEIARDAGADTFVAGSAVFRGSDPAEQIAALRAAADGH
                     RH"
     gene            complement(4616..>5595)
                     /locus_tag="PLW15_08235"
     CDS             complement(4616..>5595)
                     /locus_tag="PLW15_08235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOT34419.1"
                     /translation="TRTDAETWRERVLAEAHSGEEALALEYSHPLWVTRAFRQVLVAE
                     GREAELEPLLVADNVAPRVSLTALPGYATVDELDAHTSGYSPVGAVLDGGDPLKLPAV
                     REGRARVQDEGSALAALILSRARPISPGEQWLDLCAGPGGKAALLAAEAIQGGAILTA
                     NELVPARAELVRKALAVFETPPEVWEVDGTTVGETHPAQFDRILLDAPCTGLGALRRR
                     PEARWRKQPSDVAGLGALQSALLASGLAALKPGGLLAYVTCSPHAAETKAVVASVLRK
                     MPGVEKLDTPAVLQSLVRESLDLPEASHVQLWPHRHGTDAMFVQLLTKV"
BASE COUNT          919 a         1902 c         1901 g          873 t
ORIGIN      
        1 ctgatctcgt ggtcgaatcg ctgcgagacg accacctgga acacatcgcc gtcacggatg
       61 tactgcttcg acttctcgat ggccgccgcg taggcgtccg gagtggtgcg cgggatgggg
      121 gtgggcgcga tcgtcagatc cacctcggcg agccacgcct cgctgggccg tgacagcgcc
      181 acctgcatgt cgtcgagccg agcttgcgcg tcagaccaca gagtggacga gtcgtcgacg
      241 ccgtcgttga gcgccgcggc gatcagcaga acggttccgt accggtggtc gagcacgacc
      301 agcgagctca cgaaacccag ggcctgcccg ggcacgctga agtcggccgg gggtgcatcg
      361 gggagatgct cgatctggcg gatcgcctcc cagccgatga agcccacgag tcctccggtc
      421 agcggcgggt ggccgtccac tcgcggcgtt ccccagcgtt cgtagaggtg ggccagcgcg
      481 tcgagcggcc gtgaggggtg ctctgcaccg agcgcgcgct cctcggagag accgtaatcg
      541 agccacacca cgttctcccc ggactgggtg agcacgccga aactcgacac tccgacgaac
      601 gagaagcgcg accagattcc gccctgctcg gcggactcga ggaggaaggt accgggcttg
      661 ccctgcgcca gcttgcggta gatgcccacc ggggtctcgc cgtcggcgaa gagctcgcgg
      721 atcaccggga cgacgcgatg gccgccagcg atgcgcgcgt cgaagtcgtc gcgcgtggtg
      781 gttccgatca tgagggccct cccagagtcg gggacagagg gtcgacgtcg aagcaggagt
      841 gggcacccgt gtggcacgcg ggcccgtgct gatcgacagt gacgagcaga gcgtcagcgt
      901 cgcagtcgag cgccgccgcc cgcacgtact gcacgtgacc gctggtgtcg cccttgcgcc
      961 agtactcctg gcgactgcgg ctccagaacg tgactcggcc ttcggtgaga gtgcgccgca
     1021 gcgcctccgc gtccatgtag ccgagcatga gcacgtcctt gctgcccgcc tcctgcacga
     1081 tcgcgggaat gagaccgttg tcgccgaaga ccgcacggtc gatgacctcg ccgactgtgg
     1141 catccgtcaa ttcactcatc gcacaaccat tcccgaatcg gacagcgcct gtttcacatc
     1201 gccgacagtg agttctccat tgtggaagac ggatgccgcc aggacagcat cggcgcccgc
     1261 cgcgatcgcc ggcgcgaagt cgccgaccgc accagcccca ccactcgcaa tcaccggaac
     1321 gtggctgagt tcccgcatcg cggtcaccag ttcgaggtcg aacccgcgct tggttccgtc
     1381 ggcgtcgatc gagttcacca gcagttcgcc cgcgccccgc tcgatcgcct cccgcgccca
     1441 gtcgagcgca tcgaggtcgg tctcggtgcg accgccgtgg gtggtgacga cgaatcgccc
     1501 gccgctgcgc ttgacgtcga gactcagcac gagcacctgc gcaccgaagc ggtcggcgat
     1561 ctcaccgacc agctcgggac gcgcgatcgc ggcgctgttc acgccgacct tgtcggcacc
     1621 gctggcaaga aggcgcgcga cgtcctcggc gctgcgcact ccgccgccca cggtcagcgg
     1681 gatgaagacc tgctcggccg tggcacgaac cacgtcgtag gtcgtcgccc ggttgtcgac
     1741 ggtggccgtg acatccagga aggtgatctc gtcggcgccc tgctcgtagt acttgcgggc
     1801 gagctcgacg ggatctccgg catcgcggag gttgaggaag ttgacgccct tcaccactcg
     1861 accgtcggcg acgtcgagac agggaatcac gcggacggcg acgcccacga tcagatccga
     1921 gcggcgtgga tcgcgctcac gagaatcgcg cgagcgccga cctcgtagag ctcgtccatg
     1981 acgtggttgg catcggagcg cagcaccatg gcacgcactg caacccattc cggatcgtgc
     2041 agcggcgaga gtgtggggct ctcaagccct ggggtgatcc tcgtggccgt ctcgacgagc
     2101 gcgaccggga cgtcgtagtc gacgagaacg tactgacggg ccacgagcac accttggagg
     2161 cggcgctgca atgtggcgat gcccgcgagg tcgggtttcg cactgatgag aacggcggtc
     2221 gagtccagaa tgacgggacc gaagatctcc aggccctgag cgcgcagggt cgagccagtc
     2281 gaaaccacgt cggcgacggc atccgcgacg cccaacttga cggccgattc gacggcgccg
     2341 tccagcgaga cgatggagac ctgcacgccg ttgcgggcga ggaagtcccc gacgagggcg
     2401 gggtagctcg tggccacccg cactccggct aggtccccta gctgcgtgaa ccgacccggc
     2461 gctgccgcga aacggaacgt cgactcaccg aagtcgagcg cggcgatctc ctgtgcggtg
     2521 ctccgcgaat cgagcaacag gtcacggccc gtgatgccga catccagcgc gccagagccc
     2581 acatatgtgg cgatgtctcg tggccggagg tagaagaact cgacgtcatt gcgcgggtcg
     2641 agcgcgctca gcgctcgcag gtcacgccga ccggcgtatc ccgcctcggc gagcatctcc
     2701 gcggcgatct cactcagcga gcccttgttg ggcactgcga ttctcagcat ggttgtcttt
     2761 cgtcgaaggg tggatgcggg gacgacttac aaatgtcgcc agacatccgc cggggtgagc
     2821 cccttcgcga gcatgagcac ctgcaggtgg tagatgagct gcgagatctc ctccgccgtc
     2881 tcggcgtcgg actggaactc ggccgccatc cagacctcgg cagcctcttc gacgatcttc
     2941 ttgccgatcg cgtgcacccc cgcgtcgagc tcggcgacag tgcccgaccc ggtcggccgc
     3001 gtcaccgcct tgtcgctcaa ctcagcgaag agctcgtcga aggttttcac ccgtacagcg
     3061 tatcggctgc ccggaggccc gggcgcccgg ctacttgacg cccagcaggt cgatcacgaa
     3121 cgtgagggtg cgtcccgaga gacggtgacc gccaccggcg ggaccgtagg cgagcgcagg
     3181 cgggcagatg agctggcgac ggccgccgac cttcatgccg gggatgccct cctgccagcc
     3241 cttgatgagg cccgatagcg ggaactggat cgactggccg cggttccagc tggagtcgaa
     3301 ctcctcgccg gtctcgaagt cgacgccgag gtagtgcacg tcgaccgtcg cgccgggctg
     3361 ggcgacctcg ccggtgccct cctcgagatc gatgatcacg agctcggtgg gctccggacc
     3421 ctcgtagaag tcgacctcgg gcttggttcg cggtgaatct gtcatgctct aatcgtgcca
     3481 tcaaatgacg aaacagctct cccccatctg atgagggccg tcgctctcgc gtcgtcggcc
     3541 cgcgagcacg gcaatgatcc gttcggggcg ctcctggtca ccgagtccgg cgtggtcgtc
     3601 gaggccgaga acacggtcgt gacggccagc gaccccaccg gacacgccga gaccaacctc
     3661 gtgcgccttg cggcggcgca gctcacgccc gcggagctca cgactgcgac cctgtacacg
     3721 agcaccgaac cctgcgcgat gtgctgcgga gccatcttct gggcgggtat cggtcgcgtg
     3781 gtctacgcgc tcgccgagtc ggagctcaac gccatgctcc cacccgagtc gatcggcctc
     3841 gtgctcgacc tgcccagccg ggaggtcttc gcccggggcg gccaccagac cgaggtcatc
     3901 ggtccgatcg ctgtgcccgg cgctgccgag gtgcacgcgg gctactggag ctagtggcgg
     3961 tgtccgtccg cagccgcgcg cagggccgcg atctgctcgg cggggtcgga gccgcggaag
     4021 accgctgacc cggcgacaaa ggtgtcggcg cccgcgtcac gggcaatctc aatcgtcgtg
     4081 agatcgaccc cgccgtcgac ctggagccag acatccgtgc cggctctggt gatcgcctca
     4141 cgcacctgcg cgagtttggg catggtggag accatgaagc tctggccacc gaagcccggc
     4201 tcgacagtca tgatcagcac ctggtcgaac tcgtgcagga gatcgaggag aggctccgcg
     4261 gcggttccgg gcttgagtgc gatgcctgcg cgggcgccga gggagcgcag ggtgcgcgcc
     4321 agttccaccg gatgctccgt cgcctccgcg tggaaggtca ccgatgccgc gcccgtcttg
     4381 gcgtagtcgg gagcccatcg gtcgacatcg gtgatcatga ggtgcacgtc gagggggcgg
     4441 ggcgagacat cctgaagcct gccgaccatc tgcaggccga acgtgaggtt gggcacgaaa
     4501 tggttgtcca tgacgtccac gtgcacgaag tcggccgagg cgatgctggc gacgtcccgc
     4561 tgtagattca cgaagtcggc agcgaggatg ctggggttga tgcgagcgcc catggtcaca
     4621 ccttagtcag gagctgcacg aacatggcgt cggtgccgtg gcggtgcggc cagagctgca
     4681 cgtggctggc ctcgggaaga tccaacgact cccgcactag tgattgcaac accgcgggag
     4741 tgtccagttt ctccacccca ggcatcttcc tcagcacgga tgccacgacc gccttcgtct
     4801 ccgcggcgtg cggcgagcac gtgacgtacg ccaacagtcc gccgggtttc agcgcagcga
     4861 ggccggaggc tagcagtgcc gactggaggg caccgagtcc cgcgacgtca ctgggctgct
     4921 tgcgccagcg cgcctcgggt cgacggcgca gggcaccgag ccctgtgcag ggggcatcca
     4981 gcagtatccg gtcgaactgc gcaggatgcg tttcgccgac cgtcgtgccg tccacctccc
     5041 agacctcggg cggagtctcg aacacggcca gcgccttgcg cacgagttcg gcccgcgcgg
     5101 gcaccaactc gttggccgtc aggatggcac caccctggat cgcctcggcc gcgaggaggg
     5161 cggccttgcc accgggaccg gcgcagaggt cgagccactg ctcccccggg ctgatcggcc
     5221 gcgctcggct cagaatgaga gcggcgaggg ccgagccctc gtcctgaacg cgcgcccggc
     5281 cctcccgcac tgcgggcagc ttgagcgggt caccaccgtc gagcacggcg ccgaccggtg
     5341 agtacccact ggtgtgggca tcgagttcgt ccacggtcgc gtatcccggt aatgccgtca
     5401 gagacacgcg cggcgcgaca ttgtccgcga ccagaagcgg ctcgagttcg gcttcgcgcc
     5461 cctcggcgac gagcacctgc ctgaacgccc gagtgaccca gagcggatgc gagtactcca
     5521 gcgcgagcgc ctcctcgccc gagtgcgcct cggcgagcac ccgctcacgc caggtctcgg
     5581 catcggttcg ggtga
//
LOCUS       DAOSOU010000142         5564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14739_length_5564_cov_4.876021, whole genome shotgun sequence.
ACCESSION   DAOSOU010000142 DAOSOU010000000
VERSION     DAOSOU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14739_length_5564_cov_4.876021"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /gene="coaBC"
                     /locus_tag="PLW15_08240"
     CDS             <1..846
                     /gene="coaBC"
                     /locus_tag="PLW15_08240"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOT34420.1"
                     /translation="SMHTEMWQNAATVANIATLRQRGVTVVGPAVGQLTGADSGPGRM
                     SEPEEIVEVALRTAAGRQDLAGRRILVTAGGTREPIDPVRFIGNRSSGKQGVALAVAA
                     ADRGAEVTLIAANLEVDVPATVQVVRVRSTLDLEKAATSAAETADIVIMAAAVADYRP
                     ETVADAKIKKDVQGDVLDLRLVKNPDILAGLAAHRRAGQVIVGFAAETEPDDAKLRQL
                     GAAKVARKGADLLVVNRVGWNEGFATERNTVIVLNRTGDIVGEASGSKRSVADRILDL
                     LPHSI"
     gene            861..>2022
                     /gene="metK"
                     /locus_tag="PLW15_08245"
     CDS             861..>2022
                     /gene="metK"
                     /locus_tag="PLW15_08245"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT34421.1"
                     /translation="MSTDLRLFTSESVTEGHPDKICDQISDSILDAMLTEDPRARVAV
                     ETLVTTGLVHVAGEVTTSGYVDIPSIVRDKITSIGYDSSDVWFDGRSCGVSISIGSQS
                     PDIAQGVDSAFEAREESSSDAGDQQGAGDQGIMFGYATTETPELMPIPIWLAHRLAER
                     LAEVRHTGTLEYLRPDGKTQVTVGYEGSIPRTIDTVVVSTQHSPSVSNERLRAEVEEV
                     VIRPVLERSGLEFADLRVLINPTGRFEIGGPQGDAGLTGRKIIVDTYGGASRHGGGAF
                     SGKDPSKVDRSAAYAMRWVAKNAVAAGLADRLEVQVAYAIGKAAPVGLYVESFGTGHV
                     DDETIIRAIQEVFDLRPGAIIRELDLLRPIYAQTATYGHFGRELPDFTWERTD"
     assembly_gap    2023..2122
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2215..4197
                     /locus_tag="PLW15_08250"
     CDS             2215..4197
                     /locus_tag="PLW15_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT34422.1"
                     /translation="MVAAIARVMVDSPLPQLDRLFDYGIPPELASEAVPGVRVRVPLR
                     SAGRVADGYIVEVVESGDYSGALSDLESVVSPIRVLTPEVWTLARRAADRAAGSATDI
                     VRLAVPGRQVRVEKAYLADITSDPFPTVAAPAIDGYGAGVVEAALEANERLAVDAVPL
                     VQPLPDGVWVGGWAVALARAAASVLAGGRTAILAVPDYRDQEQLEAALRAILPPECIV
                     RLDARQSNPDRYRAFLRCLSDRPLAIVGNRSVVYAPAANLGLIAVWDDGDPLHSEPLA
                     PYVHARDAALLRQEQQGCALMFLGHSRSTEVERLVEVGWLRSISPVPRVLPKIVPTAN
                     QAATDRLAAGARIPSSAWQVARTALASGPVLVQVARPGYAPGLACVDCHQTARCERCE
                     GPLAQKTSRSVPACLWCGALAVEWRCSNCGGEKLRHVGQGATRTAEDLGRAFPGVRVI
                     IADGDRPILSVGAEPALVIATRGAEPVAAGGYRAVLLLDGERMVARESLRVAEDCLRW
                     WSNAVALAAGGATTVLVGVGGALASALVTWRHADFARSELADRRRLRFPPAVRVATVT
                     GTVEAVAQAVERTASALQLDRSDVLGPVDVSPTTVRTILRFDYSRGAEVAADLRAQVI
                     RAATSRRKPAVAGKGTAPPPPPLKVRFDDVEPFLDS"
     gene            4232..5134
                     /gene="fmt"
                     /locus_tag="PLW15_08255"
     CDS             4232..5134
                     /gene="fmt"
                     /locus_tag="PLW15_08255"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT34423.1"
                     /translation="MRLVFAGSPDAAVPSLRALAESAHDVVTVITREDSPQGRRAVLT
                     PTPVAVEADRLGVPVIRANRLAGKATETVQALAPDLGVIVAYGGLVREPLLSTPRLGW
                     INLHFSLLPRWRGAAPVQRAIMAGDDITGASVFQLVPELDAGDVFGRVTEPIGRHQTA
                     GSLLDSLAESGASLLVRVVDALADGTARAVPQDGDVTLAPKLGLADGEVDWTLPAEAV
                     SAQIRGVTPEPGAFTTIDDARLKILDATIARDVPRMPAGQFALVGRRVVVGTATDPIE
                     LVAVHPSGRRAMDAASWWRGRPTP"
     gene            5131..>5564
                     /locus_tag="PLW15_08260"
     CDS             5131..>5564
                     /locus_tag="PLW15_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT34424.1"
                     /translation="MSQPQPARRIALDVIMAVRESDAYANLLLPVRLGRAKLSEADAG
                     LATELTYGTLRMQGYYDRIIALAAGRSVDKIDPPILDVLRLACHQLLSMRVAQHAAVD
                     ESVQLAKTVGSNSAVGFVNGVLRTITRTDAETWRERVLAEAHS"
BASE COUNT          811 a         1887 c         1890 g          876 t
ORIGIN      
        1 tccatgcaca ccgagatgtg gcagaacgcc gcgacggtcg ccaacatcgc gacgctgcgg
       61 cagcgcggtg tcacggtcgt cggcccggcc gtgggccagc tcacgggagc cgacagcggt
      121 cccggtcgca tgtccgaacc cgaggagatc gtcgaggtgg ccttgcgcac agccgccggc
      181 cggcaggatc tcgcggggcg gcgcatcctg gtcaccgccg gaggcactcg cgagcccatt
      241 gacccggtgc ggttcatcgg caaccgatcg agtggcaagc agggcgtcgc actggccgtc
      301 gcggcagccg atcggggcgc cgaggtcacc ctcatcgccg cgaacctcga ggtcgacgtg
      361 cccgccacgg tgcaggtcgt gcgagtgcgc tcgaccctcg acctcgagaa ggccgcgaca
      421 tccgctgccg agaccgctga catcgtcatc atggccgccg ccgtcgccga ctaccgcccc
      481 gagaccgtcg ccgacgcgaa gatcaagaag gacgtgcagg gcgacgtgct cgatctgcgt
      541 ctcgtgaaga accccgacat tctggccggg ctggccgccc accggcgtgc cggacaggtg
      601 atcgtcggct tcgcggccga gaccgaaccg gatgacgcga agctcaggca actcggagca
      661 gccaaggtcg cgcgcaaggg cgccgacctg ctcgtcgtca acagggtcgg ctggaacgag
      721 ggtttcgcca cggagcgcaa cactgtcatc gtgctcaacc gcaccggcga tatagtgggc
      781 gaagccagcg gctcgaagcg gtcggtagcc gatcgtatcc tcgacctgtt accccattcg
      841 atctagccac ggagtccgcg attagcaccg acctgcgcct cttcacctcg gagtccgtca
      901 ccgaaggcca ccccgacaag atctgcgatc agatcagcga cagcattctc gatgccatgc
      961 tcacggagga tccgcgcgct cgcgtcgcgg tcgagaccct cgtgaccacg gggctggttc
     1021 acgtcgcggg tgaggtgacg acctccgggt acgtcgacat cccttcgatc gtccgcgaca
     1081 agatcacctc gatcgggtat gactcgtccg acgtctggtt cgatggtcgc tcgtgcgggg
     1141 tttcgatctc gatcggttcc cagtcgccgg acatcgctca gggtgtcgac agcgccttcg
     1201 aggcgcgaga agagtccagc tccgatgccg gcgaccagca gggcgctggc gaccagggca
     1261 tcatgttcgg ctatgcgacg acggagacgc ccgagctgat gcccatcccg atctggctgg
     1321 cccaccgtct cgccgagcgg ctcgccgagg tgcgccacac cggaacgctc gagtacctgc
     1381 gacccgacgg caagacccag gtgaccgtcg gttacgaagg gtcgattccc cgcaccatcg
     1441 acaccgtcgt cgtgtcgacg cagcactcgc cctcggtatc gaacgagcgg ctgcgagcag
     1501 aggtcgagga ggtcgtgatc cgacccgtac tcgaacgctc gggactggag ttcgccgacc
     1561 tgcgggtgct catcaatccc actggccgct tcgagatcgg cggaccgcag ggcgacgccg
     1621 ggctcacggg tcgcaagatc atcgtcgaca cctacggtgg agcgtctcgc cacggcggcg
     1681 gcgctttcag cggcaaggat ccgtcgaagg tggaccggtc ggcggcgtac gccatgcgct
     1741 gggtagcgaa gaacgctgtc gccgcaggac tcgcggatcg cctcgaggtg caggttgcct
     1801 acgcgatcgg caaggccgct ccggtgggtc tctacgtcga gtcgttcggc acgggccacg
     1861 tcgacgacga gaccatcatc cgcgcaatcc aggaggtgtt cgatctccgt ccgggcgcca
     1921 tcatccgcga gctggatctg ctgaggccga tctacgccca gaccgcaacg tacggccact
     1981 tcggtcgtga gctgcccgac ttcacctggg agcgcacgga ccnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnacgtacgg ccacttcggt cgtgagctgc ccgacttcac
     2161 ctgggagcgc acggaccggg tcgacgcact gcgcggcgca agcgggctct aaccatggtt
     2221 gcggccatcg cccgggtgat ggtcgattcg cccctgccgc aactcgaccg cctcttcgac
     2281 tacgggatcc cgcccgagct cgcctccgaa gcggtgcccg gggtgcgcgt gcgcgtcccg
     2341 ttgcgttcgg ccggccgggt cgccgacggc tacatcgtcg aggtcgtcga gtcgggtgac
     2401 tactccggtg cgctcagcga cctggagtca gtggtttcac cgatccgcgt gctcaccccc
     2461 gaggtctgga ctctcgctcg acgagctgcg gaccgggcgg cgggctccgc caccgacatc
     2521 gtgcgactcg cggtgccggg tcgacaggtg cgggtcgaga aggcctatct cgccgacatc
     2581 acgtccgacc cgtttcccac cgtggcggcg ccagcgatcg acggatacgg ggccggcgtc
     2641 gtcgaggcgg cgctggaggc gaacgagcgc ctggcggtgg atgcggttcc gctcgtgcag
     2701 cccctgcccg acggcgtgtg ggtgggcggc tgggcggtcg ccctggcccg cgccgcggca
     2761 tccgtgctgg ctggcggtcg cacagcgatc ctcgccgtgc ccgactaccg cgatcaggag
     2821 cagctggagg ctgccctccg ggcgatcctg ccaccggagt gcatcgtgcg attggatgcg
     2881 cgacagtcga atcccgaccg ctaccgcgcg tttctgcgct gcctctccga ccggccgctg
     2941 gcgatcgtcg gcaaccggtc ggtcgtctac gcacccgccg cgaacctcgg gctgatcgcg
     3001 gtgtgggatg acggcgaccc gcttcacagc gagccgctcg cgccctacgt gcacgctcgg
     3061 gatgcagcgc tgctgcgcca ggagcagcag ggttgcgcgc tgatgtttct cggccactcc
     3121 cgcagcaccg aggtcgaacg tctcgtcgag gtcggctggt tgcgatcgat ctctcctgtg
     3181 ccacgggttc tgccgaagat cgttcccacc gccaaccagg cagcgaccga ccggctcgcg
     3241 gccggggcgc gcatcccgtc gtcggcgtgg caggttgccc gcacggcact tgcctcaggg
     3301 ccggtgctcg tacaggtcgc caggcccggc tacgcgccgg gactcgcctg cgtcgactgc
     3361 caccagaccg cacgctgtga gcgctgcgag gggccgttgg cgcagaagac ttcgcgatcg
     3421 gtgcccgctt gcctctggtg tggcgctctc gcggtcgagt ggcgatgctc gaactgcggg
     3481 ggagagaagc tgcgccacgt cggccagggt gcaacgcgca cggcggagga tctgggtcgc
     3541 gccttccccg gcgtgcgggt gatcatcgcc gacggcgacc gcccgatcct gtccgtcggc
     3601 gccgaacccg cactggtgat cgccacccgg ggtgccgagc ccgtcgcggc cggtggctac
     3661 cgggccgtgc tgctcctcga cggtgaacgc atggttgcgc gcgagagcct tcgtgtggcc
     3721 gaggactgtc tgcgatggtg gtcgaatgct gtggcgctgg cggcgggggg cgccacaacc
     3781 gttctggtgg gagtgggtgg ggcactggca tccgctctcg tcacctggcg gcacgccgac
     3841 ttcgcgcgat cggagcttgc cgatcgacgg cgtctgcgct ttccaccggc ggtgcgggtg
     3901 gcaacggtca cgggaactgt cgaggccgtc gcccaggcgg tggagcgcac ggcgagcgcg
     3961 ttgcagctcg atcgctccga cgtgctcggt ccggtggatg tgtcgccgac gacggtgcgc
     4021 acgatcctgc gtttcgacta ctcccgcggc gccgaggtcg cggccgatct gcgggcgcag
     4081 gtcatccgcg ccgcaacgag ccgacgcaag ccggcggttg cgggcaaggg gactgccccg
     4141 ccgccgccgc cgttgaaggt gcgattcgac gacgtcgagc ccttcctcga cagttgaggc
     4201 cgcgccgaca cgggagaatg gatgcatgcc cttgcgcctc gtgttcgccg gaagccccga
     4261 cgcggccgtt cccagtcttc gcgccctcgc tgagtcagcg cacgacgtcg tcacggtgat
     4321 cactcgcgag gactcacctc agggacgtcg agcggtgctc acaccgacgc ctgtcgccgt
     4381 cgaggccgat cgcctgggcg tcccggtgat cagggccaat cgactcgccg gcaaggcgac
     4441 ggagaccgtg caggctctgg cgcccgatct cggcgtgatc gtggcctacg gcggcctcgt
     4501 gcgggagccg ttgctgtcga caccccggct cggctggatc aatctgcatt tctcccttct
     4561 gccccgctgg cgcggcgcag cgcccgtgca acgagcgatc atggccggtg acgacatcac
     4621 gggcgcgagc gtcttccaac tcgtaccgga gctggacgcc ggcgacgtct tcggtcgggt
     4681 gaccgagccc atcggtcgac accagaccgc ggggagtctg cttgattcgc tcgcggagtc
     4741 gggtgcgagc cttctcgtgc gtgttgttga tgcactcgcc gatggcacgg cacgtgcggt
     4801 gcctcaggat ggcgacgtca ccctggcacc caagctcggc ctcgccgatg gcgaagtgga
     4861 ctggacgctg ccggccgagg cagtgagcgc gcagatcagg ggtgtgacgc cggaacccgg
     4921 cgcgttcacg actatcgacg atgctcggct gaagatcctc gacgcgacca tcgcgcgcga
     4981 cgtccctcgg atgcctgcgg gacagttcgc gctggtcggt cggcgggtcg tcgtcggcac
     5041 ggcaacggat cccatcgaac tcgtggctgt gcatccttcg gggcgtagag cgatggatgc
     5101 cgcgtcctgg tggcgaggac ggcccacccc atgagccagc cacagcccgc ccgccggatc
     5161 gcactcgacg tcatcatggc cgtgcgtgag tccgacgcgt acgcgaacct cctgctcccg
     5221 gtgcgtctcg ggcgggccaa gctgtccgaa gccgatgcgg ggctcgccac cgagctcacc
     5281 tacggaaccc tgcgcatgca gggctactac gaccggatca tcgcgctggc cgccggacgt
     5341 tcggtcgaca agatcgaccc accaatcctg gatgtgctgc gactggcctg ccaccagctg
     5401 ctgtccatgc gtgtcgccca gcacgccgcc gtcgacgagt ccgtgcaact ggcgaagacc
     5461 gtggggtcga attcggccgt gggcttcgtc aacggtgtgc tgcgcacgat cacccgaacc
     5521 gatgccgaga cctggcgtga gcgggtgctc gccgaggcgc actc
//
LOCUS       DAOSOU010000143         5560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14749_length_5560_cov_6.728065, whole genome shotgun sequence.
ACCESSION   DAOSOU010000143 DAOSOU010000000
VERSION     DAOSOU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14749_length_5560_cov_6.728065"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLW15_08265"
     CDS             complement(<1..630)
                     /locus_tag="PLW15_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT34425.1"
                     /translation="MSYQTILLDVSDGIATLTLNRPEQLNAFNVAMMREMIDAFDRID
                     ADDAVRVVIVTGAGRAFCSGADLSAGSDSFDYEELAGSDPEFAAAKASGTEGESKVTR
                     DGGGLLTLRIFRCTKPVIGAINGHAVGIGATMTLPMDIRMATAGAKIGFVFGARGIVP
                     EAASSWFLPRLVGISRAAEWIYTSRVFLAAEGHEHGLIRSVHDTPADLLD"
     gene            648..1373
                     /locus_tag="PLW15_08270"
     CDS             648..1373
                     /locus_tag="PLW15_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOT34426.1"
                     /translation="MPDESASEVHDPPDRALRDGLALAFAVGLVGIVFGVLARSSGLS
                     VAKACAMSLLVFTGASQIAAASVIGAGGTQYAALGSALLLAARNALYGPVVAPWFRES
                     STLTRVGLAHVVIDESTGMGAAQTTDQQRRTGFLAAGLGIYVAWNLGTLLGAAAGDLI
                     GDLEAWGLDAAFPAVYVAMLAPHLRSRPGKVSALVAGAVAVATVPVLPVGIPILVCTI
                     GALVGAVAAHRDRERELDEAAHR"
     gene            1370..1672
                     /locus_tag="PLW15_08275"
     CDS             1370..1672
                     /locus_tag="PLW15_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOT34427.1"
                     /translation="MSWWVIFGLAAGAYALKAFGLLGLSRIELTGPLADLVRLLPAAM
                     FAGLVMQQTLTGAAAEVIATRSIGVAAGALAVWAKAPLLVVILVSAGVTALARQFT"
     gene            complement(1760..2656)
                     /locus_tag="PLW15_08280"
     CDS             complement(1760..2656)
                     /locus_tag="PLW15_08280"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT34428.1"
                     /translation="MRLDYLARETGQNLIRNWLLSIATVLIVTVSLTMFGITVLLGFY
                     GLDNAFIRWNNDVSFIVYMNADASQDQIDAIAKDLDKSPQVDTVEYYDDDKTYELYQR
                     IFQQEKPEMIGALNKGELPTSFRVKPSNPDAAVVKELANSYRPKTGVYDVDFPDEQVR
                     QVQGAAKKIRLWLLMGVIALLAASVVLIFIAIQTAVFSRRREIEVMRLVGATNWFIRI
                     PFIVEGVIQGIVGAGIAITFTGLLGLGWKSAGSGSFKDSLLSRFVWTGSQQTTAFIVM
                     LAIGVIVGAVGSFISTLWYLRD"
     gene            complement(2663..3340)
                     /gene="ftsE"
                     /locus_tag="PLW15_08285"
     CDS             complement(2663..3340)
                     /gene="ftsE"
                     /locus_tag="PLW15_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOT34429.1"
                     /translation="MIKLENVKKVYKNDHVALRNASVSIDKGEFVFLVGPSGSGKSTF
                     IRLLNREELPTEGKVWVAGKDISSIPNWRVPLLRRNIGYIFQDFKLLPNKTVEENVAF
                     ALEAIGRPRQSVKTQVPAILELVGLAHKAKNRPDELSGGEQQRVSIARAFVNRPLILL
                     ADEPTGNLDPNTTIGIMKLLDRINRTGTTVVMATHDRGIVDVMRRRVIELEGGQIVRD
                     QARGVYE"
     gene            complement(3490..4641)
                     /gene="prfB"
                     /locus_tag="PLW15_08290"
     CDS             complement(3490..4641)
                     /gene="prfB"
                     /locus_tag="PLW15_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT34430.1"
                     /translation="MAGLASAPGSLVAAMQDFSTALSDLSRRLAEARVYLRVEELTAR
                     RPQLETELARPDLWDDQEAAQKIQREFSSISDDLATYDGLAEAIDDASVLAELAREEG
                     DESQEPEINAAIAALVTRFDELELRALFTGEWDENDAIMEFNSGAGGVDAQDWCEMLM
                     RMFLRWAEQRGFEVEIDAASPGTEAGISSATFIVKGRYAYGYLRSEHGVHRLVRISPF
                     DNAARRQTSFASIKITPFIEDTSGEVEIDEKDLRIDVYRSSGAGGQHVNVTDSAVRIT
                     HLPTGIVVSCQNERSQHQNKDQAMKVLKAKLLDVERQKHQARLNEITGDQLDANFGSQ
                     IRSYVLHPYQQVKDLRSNVEVGNVDGVLDGDLDGLMEGYLRWERANASV"
     gene            complement(4728..>5560)
                     /locus_tag="PLW15_08295"
     CDS             complement(4728..>5560)
                     /locus_tag="PLW15_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT34431.1"
                     /translation="VEQRNAEIRKNVLKYDEVMNEQRKVIYARRDQVLDGMDLSEDAL
                     GSLADAVDSTIGTYCTGDFAEEWDTHGLRTEILTYWPTEIQVEQLGACTSTDEIYEIL
                     MADATRHYAEREAELGSESMREVERQVMLRIIDQRWRDHLREMDYLQDAIGLRAMGQK
                     DPLTEWQREGFEMFEQLITTVGHEFVKYIMHVQVVVEDPAPVVTNVSESGPDESAGAA
                     SISRAAAAEAVETGAPVPQDEPEAMQPVVKDSEYDKVGRNDPCPCGSGKKFKMCHGKA
                     "
BASE COUNT          850 a         1841 c         1897 g          972 t
ORIGIN      
        1 gtcgagcagg tcggcgggcg tgtcgtgcac actccggatc agcccgtgtt cgtggccctc
       61 ggcggcgagg aacacccggg acgtgtagat ccattcggcg gcccgggaga tcccgaccag
      121 ccgcggcagg aaccagctcg acgccgcctc ggggacgatg ccgcgggcgc cgaacacgaa
      181 gccgatcttc gcaccggccg tcgccatgcg gatgtccatc ggcagggtca tcgtggcgcc
      241 gatgccgacc gcgtggccgt tgatcgcccc gatcacgggc ttggtgcacc ggaagatgcg
      301 gagcgtcagg aggcctccgc cgtcgcgggt caccttggat tccccctcgg ttccggacgc
      361 cttggcggcg gcgaactcgg ggtcggagcc ggcgagctcc tcgtagtcga aggagtcact
      421 ccctgcggag aggtcggctc cggagcagaa cgcccgaccg gcaccggtca cgatcacgac
      481 ccgaacggcg tcgtcggcat cgatgcgatc gaaggcgtcg atcatctccc gcatcatcgc
      541 gacgttgaag gcgttgagct gctcgggacg gttcagggtg agggtcgcga tgccgtcgga
      601 aacgtcgagg aggatggtct ggtagctcac atagggtgtc tactccaatg cccgacgagt
      661 ccgcctcgga agtccacgac ccccctgatc gcgcccttcg cgacggtctt gccctggcgt
      721 tcgccgtcgg cctggtcggc atcgtgttcg gtgtgctggc gcggtcatcg ggcctgtcgg
      781 tcgccaaggc ctgtgccatg tcactgctgg tgttcacggg cgcctcccag atcgccgccg
      841 cgagcgtgat cggcgccggt ggcacgcagt acgcggccct cgggtccgcc ctcctgctcg
      901 ccgcccgcaa cgccctgtac ggacccgtgg tcgccccctg gtttcgtgag agctcgacgc
      961 tgacccgggt cgggctcgcg cacgtggtca tcgacgagtc gaccgggatg ggagccgccc
     1021 agacgaccga ccaacaacga cggaccggct tcctggccgc cgggctcggc atctacgtgg
     1081 cctggaacct cggcacgctg ctcggcgccg cggccggcga cctgatcggc gatctcgagg
     1141 cgtgggggct cgacgctgcg ttccccgcgg tctacgtagc gatgctcgcc ccccatctcc
     1201 gttcccggcc gggcaaagtc agcgcgttgg tcgccggagc cgtcgccgtg gcgacggtgc
     1261 cggtgttgcc cgtcggcatc ccgatcctgg tgtgcaccat cggcgccctc gtcggtgcgg
     1321 tcgccgccca ccgggaccgg gaacgtgaac tcgacgaggc ggcgcaccga tgagctggtg
     1381 ggtgatcttc ggtctggccg ccggcgccta cgcgctcaag gcgttcggtt tgctcgggct
     1441 cagccgcatc gagctcaccg gaccactcgc cgacctggtc cggctgttgc cggcggcgat
     1501 gttcgccgga ctcgtcatgc agcagacgct cacgggcgcc gcagcggagg tcatcgcgac
     1561 gcgctcgatc ggcgtcgccg ccggagcgct cgcggtctgg gccaaggcac cgttgctcgt
     1621 ggtgatcttg gtcagcgccg gcgtgaccgc cctcgcgagg cagttcacgt aaggcggatc
     1681 ggccgaggta ccggagcgat agccggagcg atagcaggag cgcagcgacg taccgagcgg
     1741 aggtggcctc ggcccagcat cagtcccgca ggtaccagag ggtcgagatg aacgagccca
     1801 cggcgccgac gatgacgccg atggccaaca tcacgatgaa cgcggtggtc tgctgggaac
     1861 ccgtccagac gaaccgagac aagagcgagt ccttgaacga gccgctgccc gcgctcttcc
     1921 atcccagccc gagcagaccg gtgaatgtga tggcgatgcc ggcacccacg atgccctgga
     1981 tcaccccttc gacgatgaag ggtattcgga tgaaccagtt ggtggcgccc accaaccgca
     2041 tcacctcgat ctcacgccgt cgggagaaca ctgcggtctg gatggcgatg aagatcagca
     2101 cgaccgacgc cgccagcagg gcgatcacgc ccatcagcaa ccacaggcgg atcttcttcg
     2161 cggcgccctg cacctgacgg acctgctcgt cggggaagtc gacgtcgtac acgccggtct
     2221 tcgggcgata gctgttggcc agctccttca ccactgcggc gtcgggattg gagggcttca
     2281 cccggaacga cgtcgggagc tcgcccttgt tgagcgcccc gatcatctcg ggcttctcct
     2341 gctggaagat ccgctggtag agctcgtagg tcttgtcgtc gtcgtagtac tcgacggtgt
     2401 cgacctgggg gctcttgtcg aggtccttgg cgatggcgtc gatctggtcc tgcgacgcgt
     2461 cggcgttcat gtagacgatg aacgacacgt cgttgttcca acggatgaac gcgttgtcga
     2521 ggccgtagaa gcccaacagc acggtgatgc cgaacatcgt gagcgagacc gtcacgatga
     2581 gcacggtcgc gatggacaac agccagttgc ggatgaggtt ctggccggtc tcccgggcca
     2641 ggtagtcgag acgcatggcc atctactcgt acaccccacg cgcctggtcg cggacgatct
     2701 ggccgccctc gagttcgatc acccgacgtc gcatcacgtc gacgatgccg cggtcgtggg
     2761 tggccatcac caccgtcgtg ccggttcggt tgatgcggtc gagcagcttc atgatgccga
     2821 tcgtcgtgtt gggatcgagg ttgccggtgg gctcgtcggc caacaggatc agcggccggt
     2881 tgacgaaggc ccgggcgatc gacacccgct gctgctcacc gccggacaac tcgtcggggc
     2941 ggttcttggc cttgtgggcc aaaccgacca gctcgaggat cgccggcacc tgcgtcttca
     3001 cactctgacg gggccgaccg atcgcctcga gcgcgaacgc cacgttctcc tccaccgtct
     3061 tgttggggag gagcttgaag tcctggaaga tgtacccgat gttgcggcgc aggagcggca
     3121 cccgccagtt cgggatggaa ctgatgtcct tgccggcgac ccacaccttg ccttcggtgg
     3181 ggagctcctc acggttgagg aggcggatga aggtggactt gcccgacccc gacggaccga
     3241 ccaggaagac gaactccccc ttgtcgatcg acacgctggc gttgcgcagc gcgacgtggt
     3301 cgttcttgta gaccttcttc acgttttcga gcttgatcac gagctatcga cgtccttggg
     3361 gcagcaactg ccgcggcggg aaggggcggg gaaaccggcg cgagcgacag gggccggttc
     3421 tgcgagcggc acagactcta ccggcacgcc cggggccgcc tggggcaccc ctcacggggt
     3481 accggaccat cacaccgagg cgttggcccg ctcccagcgg aggtacccct ccatcagacc
     3541 gtccagatca ccgtcgagca caccgtcgac gttgcccacc tcgacgttgg atcgaaggtc
     3601 cttgacctgc tggtaggggt gcagcacgta gctgcggatc tggctgccga agttggcgtc
     3661 gagctgatcg ccggtgatct cgttgagccg ggcctggtgc ttctgacgtt cgacgtcgag
     3721 gagcttggcc ttcagcacct tcatggcctg gtccttgttc tggtgctggc tgcgctcgtt
     3781 ctggcacgac acgacgatgc cggtgggcag gtgggtgatg cgcaccgccg aatcggtcac
     3841 gttgacgtgc tggccgccgg cgcccgagct gcggtacacg tcgatgcgca ggtccttctc
     3901 gtcgatctcg acctcgcccg acgtgtcctc gatgaacggg gtgatcttga tcgacgcgaa
     3961 cgacgtctgg cggcgggcgg cgttgtcgaa cggagagatg cgtacgagac ggtgcacccc
     4021 gtgttcgctg cggaggtagc cgtacgcgta gcggcccttc acgatgaacg tggccgagct
     4081 gatgccggcc tccgttcccg gagaggccgc gtcgatctcg acctcgaagc cacgctgctc
     4141 cgcccagcgg aggaacatgc gcatgagcat ctcgcaccag tcctgggcgt cgacgccgcc
     4201 ggcgccggag ttgaactcca tgatggcgtc gttctcgtcc cattcgccgg tgaagagcgc
     4261 ccgcaactcc agctcgtcga agcgggtgac gagcgccgcg atcgcggcat tgatctccgg
     4321 ctcctgggac tcgtcgcctt cctcacgggc cagctcggcg agcaccgacg catcgtcgat
     4381 ggcctcggcc aggccgtcgt aggtggcgag gtcgtcgctg atcgaggaga actcgcgctg
     4441 gatcttctgc gccgcctcct ggtcgtccca gaggtcgggg cgcgccagct cggtctcgag
     4501 ctgggggcgg cgtgccgtca gctcctcgac gcggaggtag acgcgcgcct cggcgagtcg
     4561 gcgcgagagg tcggagagag cggtgctgaa gtcctgcatg gcggcaacga ggctaccggg
     4621 tgcggaggcg agacccgcca ccgatgcacg ggctaccgag ccgaggtgcc ggagcgagag
     4681 tagggagcga agcgaccgta cctagcgacg gtggccgagg ccctcgatca ggccttgccg
     4741 tggcacatct tgaacttctt gcccgatccg cacgggcagg ggtcgttgcg cccgaccttg
     4801 tcgtactcgg agtccttgac gacgggctgc atcgcctcgg gctcgtcctg cggaaccggc
     4861 gcccccgtct cgacggcttc ggcagcggcg gcccgggaga tcgaagctgc tccggcggac
     4921 tcgtcgggcc ccgactcgct gacgttcgtc acgacggggg ccggatcctc caccaccacc
     4981 tgcacgtgca tgatgtactt cacgaactcg tgcccgacgg tcgtgatgag ctgctcgaac
     5041 atctcgaagc cttcacgctg ccactcggtg agcgggtcct tctgacccat ggcacgaagg
     5101 ccgatcgcgt cctgcaggta gtccatctcc cgcaggtggt cgcgccaccg ctggtcgatg
     5161 atgcgcaaca tgacctggcg ctcgacctcg cgcatcgact ccgagccgag ctcggcctca
     5221 cgctccgcgt agtgccgcgt ggcgtcggcc atcaggatct cgtagatctc gtcggtcgac
     5281 gtacaggcac cgagttgctc gacctgaatc tcggtcggcc agtaggtcag gatctcggtg
     5341 cggagaccgt gggtgtccca ttcctcggcg aagtcgcccg tgcaataggt gccgatggtg
     5401 ctgtcgacgg cgtcggcgag cgacccgagg gcgtcctcgg acaggtccat gccgtcgagc
     5461 acctggtcgc gccgggcgta gatcaccttg cgctgctcgt tcatgacctc gtcgtacttg
     5521 agcacgttct tgcggatctc ggcgttgcgc tgctcgacgg
//
LOCUS       DAOSOU010000144         5556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14762_length_5556_cov_5.541538, whole genome shotgun sequence.
ACCESSION   DAOSOU010000144 DAOSOU010000000
VERSION     DAOSOU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14762_length_5556_cov_5.541538"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLW15_08300"
     CDS             complement(<1..620)
                     /locus_tag="PLW15_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT34432.1"
                     /translation="MTDTQPTPATRSRRGPSPIFLGLVAIALLSGGALFLGIGAPGIV
                     AFFFVLSAWVITLCLHEFAHALVAYRNGDESVVHRGYLTLDPLRYTHPVFSIVLPLIF
                     VLLGGIGLPGGAVFVDHRALRTRGARSRVSAAGPLTNLAIAIVLAVVLAVGIGMDAPR
                     GVNAGMPHLDFWAALAFLLFLQVTATVLNLLPIPGLDGFGIIEPYLP"
     gene            complement(617..1246)
                     /gene="rsmD"
                     /locus_tag="PLW15_08305"
     CDS             complement(617..1246)
                     /gene="rsmD"
                     /locus_tag="PLW15_08305"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOT34433.1"
                     /translation="MTRIIAGFAGSLRLAVPGSGTRPTSDRVREAIFSALEARDAIAG
                     MRCLDLYAGSGALGLEAASRGAAHITLVDTKVSVAKQNAALIAKQAPRGVKPVIETSS
                     QAVQPFLAGARTFWDLVFIDPPYELSSDEVTQNLEALTPRLTPGAVVVLERSSRSAQP
                     VWPAALVLDRRKDYGDTVLYWLELAGEELGGDELGGELGGDKRDGDEIS"
     gene            1289..>2799
                     /locus_tag="PLW15_08310"
     CDS             1289..>2799
                     /locus_tag="PLW15_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT34434.1"
                     /translation="MESPLDVKLSAALGGRTAAAFDKAFGLTTVGDLLSHYPRRYARR
                     GELTALTQLPIDENVTIVAEVLEVRSRSMQARRGSILEVRISDGKGILTLTFFNQAWR
                     ANELKPGVRGIFAGKVGDYRGTLQLAHPDYELFDATDDRADSAAAKAWAELPIPIYPA
                     TSTVASWQVQKSIAVVLDTLPPIEDPVPDAVRSARALMPFRRALELVHRPTTDADWAT
                     ARESLRFQEAFVLQAALLQQRQRLRSVAATVRAPGALLSGFDETLPFTLTGDQVAVGE
                     AIAADLEAAVPMNRLIQGEVGSGKTLVALRAMLAVAESGGQSALLAPTEVLAHQHLRS
                     IVRTLGPDLSARLRPTLLTGQLPVAERRKALLASVSGSARIVVGTHALLSDNVEFFDL
                     GLVVVDEQHRFGVDQREALRLKGAQPPHVLVLTATPIPRTVAMTVFGDLDISSIAELP
                     AGRSPISSHVVPLAEKPGWMTRVWQRVAEELAQGRQAFVVCPAIDSSHVEQGEA"
     assembly_gap    2800..2899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2900..3723
                     /locus_tag="PLW15_08315"
     CDS             <2900..3723
                     /locus_tag="PLW15_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT34435.1"
                     /translation="AFVVCPAIDSSHVEQGEAPVEPVGSSGKRKPPVERDGSRRPVVE
                     LVETSAAASRTPRVEPVETSAAASRAIASVTETLATLRANPALASRRIEALHGRLPSD
                     EKDALMRAFAAGEIDVLVATTVIEVGVDVPNASAMVILDADRFGVSQLHQLRGRVGRG
                     SVPGVCLFVTGAEEGTLARERVEAVASTLDGFELANRDLELRQEGDVLGSVQSGGRSS
                     LRLLRVARDGDLISEARELAATVLAEDPELGGHPALREALARRLDEAAEAFLAKS"
     gene            3782..4270
                     /gene="coaD"
                     /locus_tag="PLW15_08320"
     CDS             3782..4270
                     /gene="coaD"
                     /locus_tag="PLW15_08320"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT34436.1"
                     /translation="MSRIAVVPGSFDPVTLGHLDVIERAAGLFDELHVVVVHNPGKTA
                     LLPIAQRVSLIQESIREAGLPASILVTSWSVGLLVDYCTDVGAIALVKGIRSQIDVAY
                     ETPMAIVNRDLAGVETVFLLPDPAHAHVSSSLVRQVAALGGDVAPYVTPAVARYLQSP
                     RE"
     gene            complement(4366..>5556)
                     /locus_tag="PLW15_08325"
     CDS             complement(4366..>5556)
                     /locus_tag="PLW15_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT34437.1"
                     /translation="LAQRRGVRTPEALVQQVTGTSSQSARRLVRVGTLVADLTAHDAG
                     GSALAEPWLEPVLRRVTAGELSAEALEAIRSGLGAPSDSIGVDALTEAARSLAHEATA
                     VSLETLAAHARELRDQLDVASVAEREQQRRAARSLRLFPQLDGMTRMVGLLDPESAAV
                     ITSAIDAVTSPRRGGPRFVDPEEAAQAAALVSDPRTTEQVALDALVEFVDVAVRARGS
                     KLLGVRRADVRVIVTQHDLDRRTGAGFLDGQTAAVSIDTVERHVCDGGYVPILFDRDG
                     AALNLGRAQRLHDARQRVVIATRDGGCIAADCDRPASWCEVHHIVEFGKGGETSVDDG
                     VLLCRHHHMLVHNNGWRVTRLDHDYWMIPPPDIGEPMILHTKSPAMRRMLGVQQREPA
                     LAGG"
BASE COUNT          824 a         1856 c         1904 g          872 t
ORIGIN      
        1 ggcaggtagg gctcgatgat cccgaagccg tcgaggccgg gaatgggcag caggttcagc
       61 acggtcgccg tcacctgcag gaacagcagg aatgcgagcg ccgcccagaa gtcgaggtgg
      121 ggcatcccgg cgttgacgcc gcggggggca tccataccga taccgaccgc taagacaacc
      181 gcaagcacga tcgcgattgc gaggttcgtg agcggacccg ccgccgacac cctgcttcgc
      241 gcgccccggg tgcgcaacgc gcgatggtcg acgaacacgg ccccgccggg aaggccgatg
      301 ccacccagca gcacgaagat gagcggcagc acgatgctga acaccggatg cgtgtaccgc
      361 agcggatcga gagtcaggta cccgcggtgc accaccgatt cgtcaccgtt gcggtaggcg
      421 acgagagcgt gcgcgaactc gtgcaggcac agcgtgatga cccaggcgga gagcacgaag
      481 aagaacgcca cgatgcccgg ggcgccgatc ccgaggaaga gtgctccgcc ggagagcagc
      541 gcgatcgcga cgagcccgag gaagatcggg ctcggaccgc gtcggctgcg ggtggcgggg
      601 gtgggttgcg tgtcggtcat gagatctcgt ccccgtcccg cttatcgccg ccgagttcgc
      661 caccgagctc gtccccaccc agctcctccc cagccaactc gagccagtac aacacggtgt
      721 cgccgtagtc cttgcgtcgg tcgagcacta gagccgcagg ccacaccggc tgggcactgc
      781 gagagctgcg ctccagcacg actaccgcgc cgggcgtgag tcggggggtc aaggcctcga
      841 gattctgcgt cacctcgtcg gagctcagct cgtacggcgg gtcgatgaac acgagatccc
      901 agaacgtgcg agcgcccgcc aggaacggct gcaccgcctg gctgctcgtc tcgatcacgg
      961 gcttcacacc gcgcggcgcc tgcttggcga tgagcgccgc gttctgcttc gccacgctga
     1021 ccttggtgtc gacgagtgtg atgtgcgccg ccccgcggga cgcagcctcc agaccgagag
     1081 cgccagaacc cgcgtagagg tcgaggcagc gcatcccggc tatcgcatct ctggcttcga
     1141 gcgccgagaa gatggcttca cgcacccgat cgctcgtggg gcgggtgccc gagccgggca
     1201 cggcgaggcg cagcgaaccc gcgaacccgg cgatgatgcg cgtcatgcag ctaacgctag
     1261 cgtcgtggaa cgcggctagc ctgaatgcgt ggaatccccc ctcgacgtca agctcagcgc
     1321 ggccctcggc gggcgcacgg cggcggcgtt cgacaaggcg ttcgggctga cgacggtggg
     1381 cgatctgctc agccactacc cccggcgcta cgcccgccgc ggggaactca ccgcgctcac
     1441 ccagctgccg atcgacgaga acgtgaccat cgtcgccgag gtgctcgagg tgcggtcgcg
     1501 gtcgatgcag gcgcggcgcg ggtcgatcct cgaggtgcgc atcagcgacg gcaagggcat
     1561 cctgacgctc accttcttca accaggcctg gcgtgcgaac gagttgaagc cgggggtgcg
     1621 cggcatcttc gcgggcaagg tcggcgacta ccgtggcacg ctgcagctcg cgcatcccga
     1681 ctacgaactc ttcgatgcca cggacgaccg ggcggactcc gccgccgcga aggcgtgggc
     1741 cgagctgccc atcccgatct acccggcgac ctcgactgtc gcgagctggc aggtgcagaa
     1801 gtcgatcgct gtcgtgctcg acaccctgcc gccgatcgaa gacccggttc cggatgccgt
     1861 gcgctcggcc cgcgcgctga tgccgtttcg acgcgccctc gagctcgtgc accgccccac
     1921 cacggacgcc gattgggcca ccgcccgcga gagcctgcgc ttccaggagg cgttcgtgct
     1981 gcaggccgcg ctgctgcagc agcgccagcg actgcgttcc gtcgcggcga cggtgcgggc
     2041 gcccggggct ctgctctcgg gtttcgacga gacgctgccg ttcacgctga ccggcgatca
     2101 ggtagcggtc ggcgaggcga tcgccgccga ccttgaggcc gccgtgccga tgaaccgtct
     2161 catccagggc gaggtgggtt cgggcaagac cctcgtggcg ctgcgggcga tgctcgccgt
     2221 tgccgagtcg ggaggtcagt cggctctgct cgcgccgacc gaggtgctcg cgcaccagca
     2281 cctgcgctcg atcgtgcgca cgctcgggcc ggacttgtct gcgcggttgc gtcccaccct
     2341 gttgactggc cagctgcctg tggccgagcg tcgcaaggcc ctgctggcca gcgtctcggg
     2401 ttcggcacgg attgtcgtcg gcacccacgc cctgctcagc gacaacgtcg agttcttcga
     2461 cctcggactc gtcgtcgtcg acgagcagca ccgcttcggt gtcgaccagc gcgaggcgct
     2521 tcgcctgaag ggtgcgcagc cgccgcacgt gctggtgctc actgcgacgc ccatcccgcg
     2581 cactgtcgcg atgacggtgt tcggtgacct cgacatctcc tcgatcgctg agctgcccgc
     2641 gggccgttcg ccgatctcca gtcacgtggt gcccctcgcc gagaagccgg ggtggatgac
     2701 acgggtctgg caacgtgtcg ccgaagaact cgcccagggc cgtcaggctt tcgtggtgtg
     2761 tccggcgatc gactcgtcgc acgtcgagca gggggaggcn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnna ggctttcgtg gtgtgtccgg cgatcgactc gtcgcacgtc
     2941 gagcaggggg aggcgccggt ggagccggtc gggtcgtccg ggaagcgcaa gccgccggtc
     3001 gagcgtgacg ggtcccgcag gccagtggtc gagcttgtag agacctcagc cgccgcatcc
     3061 cgcaccccgc gggtcgagcc tgtcgagacc tccgccgcgg catcccgagc catcgccagt
     3121 gtgaccgaaa ccctcgccac cctgcgcgct aaccccgccc tcgccagtcg gcggatcgag
     3181 gccctgcacg gccggctacc cagcgacgag aaggacgccc tgatgcgcgc cttcgcggcc
     3241 ggcgagatcg acgtgctggt ggcgaccacc gtgatcgagg tgggtgtcga cgtgcccaac
     3301 gcgtctgcga tggtgattct cgacgccgac cggttcggtg tctcgcagtt gcaccagttg
     3361 cgcggacggg tgggacgcgg gtccgtgccc ggcgtgtgcc tcttcgtcac gggggcggag
     3421 gagggcacgc tcgcacgcga gcgggttgag gccgtggcat ccacgctcga tggtttcgaa
     3481 ctggccaacc gcgatctgga gcttcgccag gagggtgacg tgctgggcag cgtgcagtcg
     3541 ggcggtcgct cgagtctgcg gctgttgcgg gttgcgaggg acggtgacct gatctccgag
     3601 gcgcgcgagc tcgcggcgac ggtgctggca gaggacccgg agcttggcgg gcatccggca
     3661 ctgcgggagg cgctggcccg gcggctggat gaggccgcgg aggcgttcct cgccaagagt
     3721 taggtgggtt catccagcgt catgtcacag aaccgtaacg cgcgacggat agtcttgacc
     3781 catgagcagg atcgccgttg ttcctgggtc gttcgacccc gtcaccctcg ggcatctgga
     3841 cgtgatcgaa cgagcggccg gactgttcga cgagttgcac gtcgtcgtcg tgcacaaccc
     3901 cggcaagacc gcgctgctgc cgatcgccca gcgggtttcg ctcattcagg agtcgattcg
     3961 tgaggcgggt ctgccggcgt cgatcctcgt caccagctgg agtgttgggc ttctcgtcga
     4021 ctactgcacc gacgtcggtg ccattgccct ggtgaagggc atccgctcgc agatcgatgt
     4081 cgcgtacgag accccgatgg cgatcgtcaa tcgcgacctc gccggtgtcg agacggtgtt
     4141 cctgctgccc gatccagccc acgctcacgt gtccagttct ctggtgcgtc aggttgcggc
     4201 cctcggcggg gatgtcgcgc cgtatgtcac cccggcggtc gctcggtacc tgcagtctcc
     4261 ccgcgaatag tcagctctcg ctcggaacgg tcagctctgg tcggtaccgc tcagctctcg
     4321 ctcggatagt cgggtttcgc cgcgggagct agccgctcgc gccgactacc cgcccgcgag
     4381 cgcgggttcg cgctgctgca ccccgagcat tcgtctcatc gcggggctct tcgtgtgcag
     4441 gatcatcggt tctccgatat cgggtggcgg gatcatccag tagtcgtgat cgaggcgagt
     4501 tactcgccag ccattgttgt ggacgagcat gtgatggtgt cggcagagca gcaccccgtc
     4561 gtcgactgat gtctcgccac ctttgccgaa ctccacgatg tggtgaactt cgcaccagct
     4621 tgcggggcgg tcacagtcgg ccgcgatgca tcccccgtct cgtgtggcga tcaccacccg
     4681 ttggcgtgcg tcgtgcagac gttgcgcgcg gcccaggttg agggccgcgc catcccggtc
     4741 gaagaggatg ggaacgtacc cgccgtcgca cacgtggcgc tcgacggtgt cgatggacac
     4801 ggcggccgtc tgtccgtcga ggaagccggc accggtgcga cgatccaagt cgtgctgggt
     4861 gacaatcacc cgcacatcgg cccgacggac gcccaagagc ttgctaccgc gggctcgcac
     4921 tgcgacatcc acgaactcga ccaacgcgtc gagtgcgacc tgttcggtcg tgcgcgggtc
     4981 gctgacgagc gcggccgcct gcgcggcttc ttctgggtcg acaaagcgcg gtccgccgcg
     5041 ccggggcgac gtgacggcat cgatcgctga ggtgatgacc gccgctgatt ccggatcgag
     5101 caggccgacc atgcgggtca tgccgtcgag ctgcgggaag aggcgaaggg agcgagccgc
     5161 ccgccgctgc tgttcgcgct cggcgacgct tgcaacgtcc agctggtcgc gcagctcccg
     5221 ggcgtgcgcg gcgagagtct cgagcgagac agcggtcgcc tcgtgggcga gggaccgcgc
     5281 ggcttcggtg agggcatcca ccccgatcga atcgcttggc gcacccaacc cggagcggat
     5341 cgcctccaga gcctcggccg acagctcacc ggccgtcacc cgacgcagca ccggctcgag
     5401 ccacggctcg gcaagcgcgc tgccgccagc atcgtgagcc gtgaggtcgg cgaccagagt
     5461 gcccaccctc acgagtcgtc gcgcggactg cgaactcgta cccgtgacct gctgcacgag
     5521 agcctccggc gtgcgcactc cgcgccgctg agccag
//
LOCUS       DAOSOU010000145         5516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14871_length_5516_cov_5.800037, whole genome shotgun sequence.
ACCESSION   DAOSOU010000145 DAOSOU010000000
VERSION     DAOSOU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14871_length_5516_cov_5.800037"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLW15_08330"
     CDS             <1..350
                     /locus_tag="PLW15_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT34438.1"
                     /translation="PVLEPLGLTHPQYLVMLALWERSPRTVRDLGETLLLEPATLSPL
                     LKRLEASGLLTRGRNAVDERTLDVELTAAGRALRVEAEKVPAKIVSRLGMQVSELERI
                     RDDLTTLISAATR"
     gene            complement(347..1369)
                     /locus_tag="PLW15_08335"
     CDS             complement(347..1369)
                     /locus_tag="PLW15_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007541943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOT34439.1"
                     /translation="MTDVDVLVVGGGPVGLASAIEARLAGLTVALVEPRDGDVDKACG
                     EGLMPGALPLLARLGVDPDGMPLRGVSYRTETRRADHLFRSGEGRGVRRTTLHRALAD
                     RATELGITRVAARVEAVQQTDSTVTAAGLTARYLLACDGLHSSVRRQVGLERAVRGSR
                     RFGLRRHFAVAPWNDLIEVHWTREAEVYITPIAPGLTGVAVLGPPRTDFDTAVAAVPE
                     LAERLAGVDAVTEVRGAGPFRQRAAARTAGRVLLVGDASGYVDAITGEGLRLGFEQAH
                     VAVAAVADDTPTRYEREWRRVTRDFRVLTHGLVAAAGSPLRGGIVPAASALPGLYGAI
                     VERLAR"
     gene            complement(1366..1887)
                     /locus_tag="PLW15_08340"
     CDS             complement(1366..1887)
                     /locus_tag="PLW15_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOT34440.1"
                     /translation="MSVILYIAFVLATGVERLYELRVSKRNAATAFAQGGREYGQRHF
                     PWMVALHTGLLLACIAEVVLLDRPFIPWLGWPMLVIAVLCQAARYWIISSLGSQWNTR
                     VIVVPGAARVVARGPYRWFTHPNYVVVAVEGIALPLVHTAWITAIAFTVLNAVLLLGF
                     RIPTENRALRELT"
     gene            complement(1884..2939)
                     /locus_tag="PLW15_08345"
     CDS             complement(1884..2939)
                     /locus_tag="PLW15_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOT34441.1"
                     /translation="MSRVAAVAPVLPAFAYTQAEITAELAPLISTVPGKRAVLERMHA
                     SSGIGTRHTALPLERYRDLGDFRQANDTFIEVATDLAERALRTALADAGLEAPDVDFV
                     MFTSVTGISAPSVDALLVSRLGLRPDVKRLPSFGLGCVGGAAGIARVNDYLVGHPDEV
                     GVLLSVELCSLTLQRDDETIANFVATGLFGDGAAAVVMVGDNRAEPGPRVVDTRSSFF
                     PDTREVIGWNIGGTGFEIVLTAGVADVIERHFPVEVAAFLADNGRGIADVAAWVAHPG
                     GPRVLDAFAHSLDLPKDRFDLSWASLDRVGNLSSSAVLHVLADTITAGVTPGGTGLLF
                     ALGPGVSAEFVLLEWPS"
     gene            complement(2990..3355)
                     /locus_tag="PLW15_08350"
     CDS             complement(2990..3355)
                     /locus_tag="PLW15_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34442.1"
                     /translation="MKSLRILAAVVAVSAVVGLSACAPTQPVVAPVTVSVGDLQGATV
                     DVALNQVININTGDLAVDSYTAEIADPSILEFVQGKEDGGATFNPGLKPLKVGTTEVT
                     MTNAQGGIQPLTFTVKVTG"
     gene            complement(3410..3910)
                     /locus_tag="PLW15_08355"
     CDS             complement(3410..3910)
                     /locus_tag="PLW15_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT34443.1"
                     /translation="MIDLPPGYSIRHTVESDHLTVVEAIGRWWDTPNRAMLGLLMPRL
                     FFQFFTGTSWIVENEQGEIGAFLIGFRSQDDPQVCYIHFVGVDPALRRSGIARQLYLL
                     FFQTMKDLGCTQVRAITGSFNRRSQAFHAALGFEAHGDREIDGVLAYPDYDGPGEPRV
                     AFSRGL"
     gene            4014..5480
                     /locus_tag="PLW15_08360"
     CDS             4014..5480
                     /locus_tag="PLW15_08360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34444.1"
                     /translation="MGTLPARHFAILWQVRAQHGGMTRAVLQRSRNFVRSGVPGVEVL
                     TFDEFEGYDSVGAELRGTGELADGVSLVNLWDWLRASTPVADAAPDVTAFAPLGSEVP
                     IVGRTRTRLAGDGTTVLQVDHLRDDGSLLVSDRRDVDEPGIHGGRSVVLCDAAGRPAK
                     SWGSIWGLYRFWLDALRTGEPAIMIVDSKTSARFMATYRRRDVLTVHVVHGSHLQGDE
                     RPFAPLRATRAETFQSLEKFDRVVVLSSRQRDDIRLLLGATPNVIVVPVSIDLPRAAD
                     ARRARPMTSGIMLSSLIARKRVDHAIEAVRGSGVTLDVYGTGVESSRLAELAQTTGGL
                     VHLRGHDTQARSRLADHGFLVLASVSEGLPLVLTEAMAAGCIPIAYDVPYGPADIITD
                     RVDGLLVKSGDVSGLRDAIAWLGERSDRELRVMRRAARLTAEQYGGEAVTRRWLVELT
                     AAWTEKVPPKPRRSLVTRARSRVRRTVRPLVARVRASH"
BASE COUNT          883 a         1943 c         1902 g          788 t
ORIGIN      
        1 gaccggtgct ggagccgctc ggcctgaccc acccgcagta cctggtgatg ctcgcgctgt
       61 gggagcgcag tccgcgcacg gtgcgcgacc tcggcgagac gctgctgctg gagcccgcga
      121 cgctctctcc gttgctcaag cggctcgagg catccggtct cctcacgcgc gggcgcaacg
      181 cggtcgacga gcgaaccctc gacgtggagc tcaccgccgc cggacgcgct ctgcgtgtcg
      241 aggccgagaa ggtgcccgcg aagatcgtgt ctcggctcgg catgcaggtc agcgaactcg
      301 agcgcatccg ggacgacctg acgacgctca tctccgcggc cacccgttag cgggcgagcc
      361 gctccactat cgccccatac agaccgggca gagcggatgc cgcgggcacg atgccaccgc
      421 gcagcggcga gcctgccgcc gcaaccagac cgtgcgtgag cacgcggaag tcgcgcgtca
      481 ccctgcgcca ctcccgctca tagcgcgtcg gagtgtcatc ggccaccgcc gccaccgcga
      541 cgtgcgcctg ctcgaagccg agtcggagac cttccccggt gatcgcgtcg acgtagcccg
      601 acgcgtctcc gactagcagc acccggccgg ccgtgcgtgc tgcagcccgc tggcggaacg
      661 ggcccgcgcc acgtacctcg gtgacggcgt caacacccgc gagccgctcg gccagctcgg
      721 gaaccgccgc gacggcggtg tcgaagtcgg ttcgcggcgg gccgagcacg gcgacgccgg
      781 tgaggccggg ggcgatgggc gtgatgtaca cctcggcctc gcgggtccag tgcacctcga
      841 tgaggtcgtt ccacggcgcc acggcgaagt gccgcctcag cccgaaccgg cgcgatccgc
      901 gcacggcgcg ctccagcccg acctgacgcc gcacgcttga gtgcagcccg tcgcaggcca
      961 gcaggtaccg ggcggtgagc ccggcggcgg tcacggtgct gtcggtctgc tgcaccgcct
     1021 cgacgcgagc cgcgacgcgc gtgatgccca gctccgttgc gcgatcggcg agcgcgcggt
     1081 gcagcgtcgt gcggcgaacc cccctgccct cacccgagcg gaacaggtgg tcggcgcgcc
     1141 gcgtctcggt gcgatacgag accccgcgca gcggcatccc gtcggggtcg acgccgagcc
     1201 gggcgagcaa cggcagagcg ccgggcatca gtccctcgcc gcaggccttg tcgacgtcgc
     1261 cgtcgcgagg ctcgactagt gcgaccgtga gcccggcgag ccgcgcctcg atcgccgaag
     1321 cgagcccgac cggtccaccg ccgacgacca gcacatcgac gtcggtcatg tcagttcgcg
     1381 cagcgcacgg ttctcggtag ggatgcggaa cccgagcagc agcaccgcgt tcagcacagt
     1441 gaacgcgatc gcggtgatcc acgcggtgtg caccagcggc agtgcgatgc cctcgacggc
     1501 gacgaccacg tagttggggt gggtgaacca gcggtacggt ccgcgcgcga cgacccgggc
     1561 ggcaccgggc accacgatca cccgggtgtt ccactgcgag ccgagcgagg agatgatcca
     1621 gtaccgggcg gcctggcaga gcaccgcgat caccagcatg ggccagccca gccaggggat
     1681 gaacggtcga tcgagcagca cgacctccgc gatgcaggcc agcagcagac ccgtgtgcag
     1741 tgcgaccatc cacgggaagt ggcgctgacc gtactcccgg ccgccctggg cgaaggcggt
     1801 cgcggcattg cgcttcgaga cccgcagctc gtagagccgc tcgaccccgg tcgcgagcac
     1861 gaacgctatg tagaggatga cactcacgac ggccactcca gcagcacgaa ctcggcactc
     1921 accccggggc cgagcgcgaa gagcagaccg gtaccgcccg gcgtgacgcc ggccgtgatc
     1981 gtgtcggcga gcacatgcag cacggccgac gacgacaggt tgcccacccg atcgagcgat
     2041 gcccagctca ggtcgaacct gtccttcggc aggtcgagcg agtgcgcgaa ggcgtccaga
     2101 acgcgcgggc cgccggggtg cgcgacccag gctgcgacat ccgcgatgcc tcgaccgttg
     2161 tctgccagga aggcggcgac ctcgacgggg aagtgccgct cgatcacgtc tgcgacgccc
     2221 gcggtcagca cgatctcgaa gccggtgccg ccgatgttcc agccgatcac ctcgcgggtg
     2281 tcggggaaga acgagctgcg ggtgtcgacg acgcgggggc cgggctccgc gcggttgtcg
     2341 ccgaccatca ccacggcggc cgcaccatcg ccgaacagcc ccgtcgccac gaagttggcg
     2401 atcgtctcgt cgtcgcgctg aagggtcagc gaacacagct cgacgctcag cagaacgccg
     2461 acctcatccg gatgccccac caggtagtca ttgacccgtg cgatgcccgc ggctcccccc
     2521 acgcagccga ggccgaacga gggcaaccgc ttcacatcgg gacgaaggcc caggcgcgac
     2581 acgagcaggg cgtccacgct gggcgccgag attcccgtga ccgaggtgaa catgacgaag
     2641 tcgacatcgg gcgcttcgag gcccgcgtcg gcgagcgccg tgcgcagagc ccgctcggcg
     2701 agatcggtgg cgacctcgat gaacgtgtcg ttggcctgac ggaagtcgcc gaggtcgcgg
     2761 tagcgctcca gcggcagcgc tgtgtgtcgg gtgccgatgc ccgacgaggc gtgcatccgc
     2821 tcgagcacgg cccgcttgcc cggcacggtc gatatcagcg gagcaagctc agcggtgatc
     2881 tccgcctggg tgtaggcgaa cgcgggcagg acgggagcaa ccgccgcaac gcgtgacatt
     2941 tcccgaacct aacacggccc ggtgcactgc gtgcaggggg cttgacgcgc tagcccgtca
     3001 ccttcacggt gaacgtgagc ggctggatcc cgccctgggc gttcgtcata gtcacctcgg
     3061 tggtaccgac cttgaggggc ttcagtccgg ggttgaaggt ggcaccgccg tcctccttgc
     3121 cctgaacgaa ctcgaggatg ctcgggtctg cgatctccgc ggtgtagctg tcgactgcca
     3181 gatcaccggt gttgatgttg atcacctggt tcagggcgac gtcgacggtc gcgccctgaa
     3241 ggtctccgac actcacggtc acgggagcca ccacgggctg ggtgggagcg caggccgaca
     3301 ggcccacgac ggcggagacg gcgaccacag ccgcaaggat cctcaagctc ttcatgccgc
     3361 taattattca gggggcggat gacacgaaca aggcctctaa gtgggggttc tacaaaccgc
     3421 ggctgaacgc cacccgcggc tcgcccggtc cgtcgtagtc gggatacgcc agcaccccgt
     3481 cgatctcgcg gtccccgtgg gcctcgaacc ccagtgcggc gtggaacgcc tgcgagcgcc
     3541 ggttgaacga acccgtgatg gcgcgcacct gcgtgcatcc gaggtccttc atcgtctgga
     3601 agaacagcag atacagctgg cgggcgatgc cgcttcggcg cagggcgggg tcgacgccca
     3661 cgaagtggat gtagcagacc tgaggatcgt cctgcgacct gaacccgatg aggaacgccc
     3721 cgatctcacc ctgttcgttc tccacgatcc aactcgtgcc ggtgaagaac tggaagaaca
     3781 ggcgaggcat gagcaggccg agcatcgcgc ggttgggggt gtcccaccag cgcccgatcg
     3841 cctcgaccac ggtgaggtgg tcgctctcga cggtgtgccg gatgctgtac ccgggaggca
     3901 ggtcgatcat gccccccagc gtagacagtc ccctacgcgc gcatgtgaga actctcagcg
     3961 gaagcggtgc accgccttac cggactccca gactcgcggc ctagcctgcg accatgggga
     4021 cactgccggc acggcatttc gcgatcttgt ggcaggtgcg agcgcagcac gggggcatga
     4081 cgcgggctgt gcttcagcgg tcacgcaact tcgtgcggtc gggggtgccc ggggtcgagg
     4141 ttctgacctt cgacgagttc gagggctacg acagcgtggg ggccgagctt cgcggcaccg
     4201 gcgaactggc tgacggggtg tcgctcgtga acctctggga ttggctgcgc gcctccaccc
     4261 cggtggcgga tgccgcacca gacgtcaccg cgttcgcgcc actcgggtcc gaggttccga
     4321 tcgtcggccg cacgcgcacc cgactcgccg gcgacggcac caccgtgctt caggtcgacc
     4381 acctccgcga cgacggaagc ctgctcgtca gcgaccgacg cgatgtcgac gagccgggca
     4441 tccacggtgg ccgatctgtc gtgctgtgcg atgcggcggg ccgaccggcg aagtcgtggg
     4501 gctcgatctg gggtctctac cgcttctggc tcgacgcact gcggacggga gagccggcga
     4561 tcatgatcgt cgacagcaag accagcgcgc ggttcatggc cacctatcgc cgacgggacg
     4621 tgctcacggt gcacgtcgta cacggctccc acctccaggg cgacgaacgc ccgttcgcac
     4681 ccctacgggc gaccagggcg gagaccttcc agtccctcga gaagttcgac cgcgtggtcg
     4741 tgctctcctc ccgccagcgc gacgacatcc gactcctgct cggcgcgacg ccgaacgtga
     4801 tcgtcgtgcc cgtgtcgatc gatcttcccc gagcagcgga tgcccgccga gcccgaccca
     4861 tgacctccgg gatcatgctc tcctctctca tcgcccgcaa gcgggtggat cacgcgatcg
     4921 aggccgtgcg cggatcgggc gtcactctcg acgtctacgg caccggggtg gagagctccc
     4981 gcctggccga actcgcccag acgaccggcg gcctggtgca cctgcgaggg catgacacgc
     5041 aggcccgctc ccggctcgcc gatcacggct tcctcgttct ggcgtcggta tcggagggtc
     5101 tgccgctcgt actcaccgag gcgatggcgg ccggctgcat ccccatcgcg tacgacgtgc
     5161 cctacggccc cgccgacatc atcacggatc gcgtcgatgg cctgctcgtg aagtccggcg
     5221 acgtatccgg tcttcgggat gcgatcgcct ggctcggcga acgctccgac cgtgaactgc
     5281 gcgtgatgcg acgcgcggcg cggctcaccg ccgaacagta cggcggcgag gcggtcaccc
     5341 gccgctggct ggtcgaactc accgccgcgt ggactgagaa ggttccgccc aagccgcgtc
     5401 ggagtctcgt cacgcgagcg cgttcgcgag tgcggcgcac tgtccggccc ctcgtcgcgc
     5461 gggttcgcgc ctcgcactga ggctcgcgcg ggtgcgcgag gggggacgtg acgccc
//
LOCUS       DAOSOU010000146         5483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_14994_length_5483_cov_4.515291, whole genome shotgun sequence.
ACCESSION   DAOSOU010000146 DAOSOU010000000
VERSION     DAOSOU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14994_length_5483_cov_4.515291"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLW15_08365"
     CDS             complement(<1..412)
                     /locus_tag="PLW15_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT34445.1"
                     /translation="MDVPARTTPQANDPSFPSIWEELNWRGLVHVSTNADELRDLLAG
                     PPITYYCGFDPTAPSLHLGNLVQLLLMRRIQLAGHKPLGLVGGSTGLIGDPRPTAERT
                     LNDAAVVAEWVGYLQAQVSKFLSFEGDNAARLVNN"
     assembly_gap    430..529
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(640..1215)
                     /locus_tag="PLW15_08370"
     CDS             complement(640..1215)
                     /locus_tag="PLW15_08370"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOT34446.1"
                     /translation="MLSRPALEVAPLLLGGHLTVDDVTLRLTEVEAYQGEADPGSHAF
                     RGPTPRTAVMFGPPGHLYTYFTYGMHVCANIVCSPEGEASAVLLRAGEIVDGIEVARM
                     RRTTSRTDADLARGPARLTVALGITLADGGSSLETGRVRFTPTDSPAQFTQGPRTGVS
                     GAGGGAAFPWRFWIPGDPTVSPYKRHPKAAG"
     gene            complement(1221..1364)
                     /locus_tag="PLW15_08375"
     CDS             complement(1221..1364)
                     /locus_tag="PLW15_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34447.1"
                     /translation="MTLQNDQDPGWWRRGMTKRATTIVVVVSVAVLVVAFIIIAVNGI
                     PLF"
     gene            complement(1361..2782)
                     /gene="argH"
                     /locus_tag="PLW15_08380"
     CDS             complement(1361..2782)
                     /gene="argH"
                     /locus_tag="PLW15_08380"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT34448.1"
                     /translation="MAEAKTGDGALWGARFASGPSAELARLSRSTHFDWQLAPYDIAG
                     SHAHAKALAAAGYLSSSEETAMHAGLDELQRRVDSGELVAAPDDEDVHGALESALIDI
                     VGAELGGRLRAGRSRNDQIATLIRMYLLDHAAVIGDLVVDLIDAINAQAEKAGGAILP
                     GRTHLQHAQPVLLAHHLLAHAWPLVRDLERLRDWRGRASASPYGAGALAGSSLGLDPA
                     LVARELGLARPSENSIDATASRDVVAEFAFIAAQLGIDLSRFAEEIIIWNTREFGFVH
                     LDDAYSTGSSIMPQKKNPDIAELARGKSGRLIGNLTGLLATLKGLPLAYNRDLQEDKE
                     PVFDSVETLEVLLPAFTGMVATLTFDTQRMAELAPQGFSLATDVADWLVRQRVPFREA
                     HEISGALVRFCEDSGLDLDEPTDEQYQAVSPHLTPGVRDVLTIEGSVSSRSGVGGTSP
                     ERVAEQRTELTARVRALTSRSTL"
     gene            complement(2870..3790)
                     /gene="argF"
                     /locus_tag="PLW15_08385"
     CDS             complement(2870..3790)
                     /gene="argF"
                     /locus_tag="PLW15_08385"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT34449.1"
                     /translation="MTRHFLRDDDLTQAEQTAILDLAEQIKADRWGRKPLAGPQTVAV
                     IFDKSSTRTRVSFAVGIADLGGVPLIISTANSQLGGKETASDTARVLERQVAAIVWRT
                     YAQSGLVEMAADTTVPVINALSDEFHPCQLLADLLTIREHKGTLAGLTLAFLGDGADN
                     MVHSYLLAGATAGMHVRIACPADYMPDERIVSDADAIAVQTGGSVTIYTDPLEAVAGV
                     DVVVTDTWVSMGKEEEKAARVASLSPYKVDADMMALAKPDAIFLHCLPADRGYEVTGE
                     VIDGPQSVIWDEAENRLHAQKALLVWLLEQ"
     gene            complement(3787..4950)
                     /locus_tag="PLW15_08390"
     CDS             complement(3787..4950)
                     /locus_tag="PLW15_08390"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOT34450.1"
                     /translation="MTWQDEYSAKIMKSAALPSRELVRGEGCYVWDDTGAKYLDFLAG
                     IAVNALGHAHPVLVDAVSRQIATVAHVSNYFANPPQLELAARLTRLTGGDRVYFCNSG
                     AEAIEAAIKLARRTGRPRIIALNNSFHGRTMGSLSLTGKPALREPFEPLVPNVEHIDS
                     TIEALERAIGPDVAAVVIEPIKGEAGVIDLPEGYLAAARDITARHGALLILDEIQTGA
                     GRTGTWWAFQQAGIVPDAIAVAKGIGGGVPIGALVTFGAASDLFTAGQHGSTFGGNPL
                     ATAASNAVLGEIERAGLVDNAAVRGEQLRAAIADLPLVDEVRGRGLLIGVGLTDDVAP
                     AVVAKALELGLIINAPNDASIRIAPPLIIGDAEIAEFIDKFTAALTHVSEATA"
     gene            complement(4947..>5483)
                     /locus_tag="PLW15_08395"
     CDS             complement(4947..>5483)
                     /locus_tag="PLW15_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041464908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT34451.1"
                     /translation="AIPGSSHLAEGISGDQYGLFTGEPRGAVVDGELVDLGLVGDVVS
                     VDPQAVLLEVAAGRVPVISTVANGPDGLLNVNADSAAASLAVALNAAKLVILTDVAGL
                     YSDWPNRDSLVSIITTTELEALLPSLESGMIPKMASCLEAVAGGVAKAAIIDGREPHS
                     ILLEVFTQQGIGTEVVPA"
BASE COUNT          809 a         1780 c         1943 g          851 t
ORIGIN      
        1 ggttgttgac gagcctggcg gcgttgtcac cctcgaaact caggaacttg gagacctgcg
       61 cctggaggta gccgacccat tcggcgacca ccgccgcatc gttgagggtg cgctcggccg
      121 tcgggcgcgg gtcaccgatg agtccggtgg agccgcccac caggccgagg ggcttgtggc
      181 cggcgagctg gatgcgacgc atcagcagca gctgaacgag gttaccgaga tgaaggctcg
      241 gggccgtcgg atcgaagccg cagtagtagg tgatcggggg gcccgccagc agatcccgga
      301 gttcgtcggc gttcgttgac acgtgcacga gcccgcgcca gttcaactcc tcccagatgc
      361 tcgggaagga ggggtcattg gcctgtggtg tggtccgtgc tggcacgtca ataaccgtag
      421 cggaggtttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gatacgcgct
      541 gccgcgctac tcaacccaag ggtttcgata cgcgctgccg cgctactcaa cccaagggtt
      601 tcgatacgcg ctgccgcgct gctcaaccgg ccggcccgct cagccggcgg ccttcgggtg
      661 gcgcttgtac ggcgagactg tcgggtcgcc ggggatccag aagcgccacg ggaatgccgc
      721 acctcccccg gcaccggaca cgcccgtgcg tggcccctgc gtgaactgcg ccgggctatc
      781 ggtaggggtg aaccgcaccc gcccggtctc gaggctggat ccgccgtcgg cgagcgtgat
      841 gcccagtgcg acggtgaggc gggccgggcc ccgcgcgagg tcggcatccg tgcggctcgt
      901 cgtgcggcgc atccgggcga cctcgatgcc gtcgacgatc tcgcccgcac gcagcaggac
      961 cgcggatgcc tcgccctcgg gtgagcacac gatgttggcg cacacatgca tgccgtaggt
     1021 gaagtacgtg tacaggtgac cgggcggccc gaacatcacc gcggtgcgcg gcgtcggccc
     1081 ccggaaggcg tgcgagccgg ggtcggcctc gccctggtac gcctcgacct ccgtgaggcg
     1141 cagggtgaca tcgtcgacgg tcaggtggcc gccgagcagc aggggcgcga cctcgagggc
     1201 cggccgagaa agcagggtcc ctagaacagc gggatcccgt tgacggcgat gatgatgaac
     1261 gccaccacca gcacggccac gctcacgacg acgacgatcg tcgtcgcgcg cttggtcatg
     1321 ccgcggcgcc accagccggg atcctgatca ttctgcagtg tcacagggtg ctccttgagg
     1381 tgagggcgcg aacgcgcgcg gtgagttcgg tgcgttgttc tgcgacgcgc tcgggcgacg
     1441 tgccgcccac gcccgatcga ctggacaccg agccctcgat cgtgagcacg tcacgaacgc
     1501 ctggggtcag gtggggcgag actgcctgat actgctcgtc ggtcggctcg tcgaggtcga
     1561 gcccgctgtc ttcgcagaat cgcacgagcg ctcccgagat ctcgtgagcc tcgcggaacg
     1621 gcacccgctg gcgaacgagc cagtcggcga catccgtggc cagggagaag ccctgggggg
     1681 cgagctcggc catgcgctgc gtatcgaagg tcagtgtcgc gaccataccc gtgaaagcag
     1741 gcaggagcac ctcgagagtc tccaccgagt cgaagaccgg ctccttgtcc tcctgcaggt
     1801 cgcggttgta ggcgagcggg agccccttga gcgtcgcgag gagaccggtg aggttgccga
     1861 tcagtcgccc ggacttgccg cgcgcgagct cggcgatgtc ggggttcttc ttctgcggca
     1921 tgatcgacga cccggtcgaa taggcgtcat cgaggtgcac gaagccgaac tcgcgggtgt
     1981 tccagatgat gatctcctcg gcgaaccggg agaggtcaat tccgagctgg gcggcgatga
     2041 aggcgaactc cgcgacgacg tcgcgactgg cggtcgcgtc gatcgagttc tcgctgggac
     2101 gagcaagacc gagctcgcgc gcgacgagcg cggggtcgag acccaggctg ctgcccgcca
     2161 gggcgccggc gccgtagggc gacgcggatg cccgcccgcg ccaatcgcgc agccgctcaa
     2221 ggtcacgcac caacggccag gcgtgcgcaa gcaggtggtg ggcgagcagc acgggctgcg
     2281 cgtgctggag gtgtgtgcgc ccgggcagga tcgcgccgcc cgccttctcg gcctgagcgt
     2341 tgatggcatc gatgaggtcg accacgaggt cgccgatcac ggcggcgtgg tcgagcaggt
     2401 acatccggat cagggtggcg atctggtcgt tgcggctgcg acccgcccgc aggcggccac
     2461 cgagctcggc acccacgatg tcgatcagcg ccgactcgag ggcaccgtga acgtcttcgt
     2521 catcgggcgc ggcgacaagc tcaccgctgt cgacccggcg ttgcagttcg tcgaggcccg
     2581 cgtgcatcgc ggtctcctcg gaagagctca ggtaacccgc agcggcgagc gccttggcgt
     2641 gcgcgtgcga gcccgcgatg tcgtagggcg ccagctgcca gtcgaagtgc gtggagcggc
     2701 tgagacgcgc gagttccgcc gagggcccgc tcgcgaaccg ggcgccccac agggcgccgt
     2761 caccggtctt cgcttctgcc acggggtcgg tctccttcgg ggttgggttc agtctaggga
     2821 tgggtctgtt ggcggtttcg atacgccgct tcgcggctac tcaaccggcc tactgctcga
     2881 gcagccagac cagcagcgcc ttctgggcat gcagccggtt ctcggcctcg tcccagatca
     2941 cgctctgcgg gccgtcgatg acctcgccgg tcacctcgta gccgcggtcg gcgggcaggc
     3001 agtgcaggaa gatcgcgtcc ggcttcgcga gagccatcat gtcggcgtcg accttgtagg
     3061 gcgagaggga tgccacccgc gcggccttct cctcctcctt gcccatcgac acccaggtgt
     3121 cggtcacgac gacgtcgaca cccgcgaccg cctcgagcgg gtcggtgtag atggtcaccg
     3181 agccgccggt ctgcacggcg atcgcgtcgg catccgagac gatgcgctcg tcgggcatgt
     3241 agtcggcggg gcaggcgatg cgcacgtgca tgccggccgt cgcacccgcg agcaggtacg
     3301 agtgcaccat gttgtcggcg ccgtcgccca ggaaggcgag cgtgagaccg gcgagcgtgc
     3361 ccttgtgctc gcgaatcgtc agcaggtcgg cgagcagctg gcacgggtgg aactcgtcgc
     3421 tcagcgcgtt gatgaccggc acagtcgtgt cggctgccat ctcgaccagg cccgactgtg
     3481 cgtaggtgcg ccagacgatc gccgcgacct ggcgctccag tacgcgggcc gtatccgacg
     3541 ccgtctcctt gccgccgagc tggctgttgg ccgtggagat gatgagcggc actcccccga
     3601 ggtcggcgat gcccactgcg aatgacaccc gcgtgcgtgt cgacgacttg tcgaagatca
     3661 ccgccacagt ctgcgggccg gcgagaggct tgcgacccca gcggtccgcc ttgatctgct
     3721 cggccaggtc gaggatcgcg gtctgctcgg cctgggtcag gtcgtcgtcg cggaggaagt
     3781 ggcgggtcat gctgtggcct cgcttacgtg ggtgagtgct gctgtgaact tgtcaatgaa
     3841 ctcggcgatc tcggcgtcgc cgatgatcag gggtggagcg atacggatgc tcgcgtcgtt
     3901 gggcgcgttg atgatcagtc ccagctccag ggccttcgcc accaccgcgg gagcgacgtc
     3961 atcggtcagg ccgacgccga tcaggaggcc gcgaccgcgc acctcatcaa ccagcggcag
     4021 gtcggcgatc gctgcacgca gctgctctcc gcggaccgca gcgttgtcga ccagtcccgc
     4081 ccgctcgatc tccccgagca ccgcgttcga ggccgccgtc gccagcgggt tgccgccgaa
     4141 cgtgctgccg tgctgaccgg cagtgaacag gtcggatgcc gcgccgaagg tcacgagcgc
     4201 cccgatcggc actccgccgc cgatgccttt ggccacggcg atcgcgtcgg gcacgatccc
     4261 cgcctgttgg aacgcccacc acgtgccggt gcgaccggcg cccgtctgga tctcgtcgag
     4321 gatgagcaga gcgccgtggc gagccgtgat gtcccgtgcc gctgcgaggt acccctcggg
     4381 gaggtcgatg acgccggcct cacccttgat cggctcgatg acgacggcgg cgacatccgg
     4441 gccgatggct cgctcgagcg cctcgatggt cgagtcgatg tgctcgacgt tgggcacgag
     4501 cggctcgaac ggctcgcgca gcgcgggctt gccggtgagc gagagcgagc ccatcgtgcg
     4561 accgtggaac gagttgttca gcgcgatgat gcgcggtcgc cccgtgcggc gggcgagctt
     4621 gatggccgcc tcgatcgcct cggcgcccga gttgcagaag tagacgcggt cgccgccggt
     4681 caggcgggtg aggcgggcgg ccagctccag ctgcggcggg ttggcgaagt agttggagac
     4741 gtgcgcgacg gtcgcgatct gccggctgac ggcatccacc agcacggggt gcgcgtgccc
     4801 cagggcgttg accgcgatac cggcgaggaa gtcgaggtac ttcgcgccgg tgtcatccca
     4861 gacgtagcag ccctcgccgc gtacgagctc gcgcgacggc agggcggccg acttcatgat
     4921 ctttgccgaa tactcgtcct gccacgtcat gcgggtacca cctccgtgcc gatgccctgc
     4981 tgggtgaaga cctcgagcag gatcgagtgc ggctcgcggc cgtcgatgat ggccgccttc
     5041 gcgactcccc cggcgacggc ctccaggcac gacgccatct tggggatcat gcccgactcg
     5101 aggctgggca gcagggcctc gagttcggtc gtggtgatga tcgagacgag cgagtcgcgg
     5161 ttgggccagt cgctgtagag cccggcgaca tcggtgagga tcacgagctt cgcggcgttg
     5221 agcgccacgg ccagcgacgc ggcggccgag tcggcattca cgttgagcag gccgtcgggc
     5281 ccgtttgcaa ccgtcgagat gacgggcacg cgaccggcgg ccacctcgag cagcaccgcc
     5341 tgcgggtcga ctgagaccac gtcgccgacc agtccgaggt cgacgagctc tccgtcgacg
     5401 accgcgcccc ggggctcgcc ggtgaagagg ccgtactggt cgccggagat gccctcggcg
     5461 aggtggctgc tgcccgggat cgc
//
LOCUS       DAOSOU010000147         5444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15118_length_5444_cov_6.949527, whole genome shotgun sequence.
ACCESSION   DAOSOU010000147 DAOSOU010000000
VERSION     DAOSOU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15118_length_5444_cov_6.949527"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLW15_08400"
     CDS             complement(<1..377)
                     /locus_tag="PLW15_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide 2'-phosphotransferase"
                     /protein_id="HOT34452.1"
                     /translation="MARSHLTLAALATSAVPGLDIAAAASFGAGQGSDFDAAIITGRD
                     GRHWIIRVPRNPRAEAEQSADLVALRALSTGVRTRLPFAVSAFAGQVPVDSTRGIVYE
                     FVYGTKTPLDSYTAGPDSLAASVG"
     gene            446..1375
                     /gene="nudC"
                     /locus_tag="PLW15_08405"
     CDS             446..1375
                     /gene="nudC"
                     /locus_tag="PLW15_08405"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOT34453.1"
                     /translation="MSQSFLSRLPLSRFELDRDHLGRERPALFDELWANPATRILPIF
                     QGSALLATPDSLALLPVDAVPHFSERVYLGTSTSTTSPEPAGTPIVAVELDDAGHLAE
                     SDWANLRQVGSRLSDRDAGLLTEALGILNWHTSHRFSPRTGEPTVSEKGGWVRRDPVS
                     GGEVFPRTDPAIIVGVTDADDRILLGSNALWESNRYSLLAGFVEPGESLESAVMREVH
                     EESGLRVVDPVYLGSQPWPFPASLMVGFTARVEDGFTGAATPDGTEILDLRWFSRAEL
                     AMALDEIRLPGPTSIARAIVEHWYGSSIDDGQR"
     gene            1372..3042
                     /locus_tag="PLW15_08410"
     CDS             1372..3042
                     /locus_tag="PLW15_08410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOT34454.1"
                     /translation="MTTPAALLDGLDDQQRVAAEALLGPVCLLAGAGTGKTRAITHRI
                     AYGVATGVYPPGRVMALTFTARAAGELRGRLRALGAGGVAARTFHASALAQLNFFWPQ
                     VIGGTMPRLLDSKARLIAHAADGLKLKLDTATLRDAAAEVEWRKTSRLSVEQYATRTR
                     TLPTSLPMDRMVDLQRAYEDLKDERRQLDFEDVLLAAAGMIEAEPRVAQQVREQYRFF
                     VVDEYQDVSPLQHDLLELWLGDHDDLCVVGDASQTIYSFAGASPEYLLGFAGEHENAV
                     VLRLEQNYRSAPPIIDAANRLMRGRPGALSLTAASELTGPRPTVNTYDSDSSEARAVA
                     AAIAEEIASGIPPEQIAVLYRINVQSQAIETALGDAGVPCVVRGATRFFDQREVKEAL
                     MLLRGATVTTSIEPLFKTVSDVLRSIGWSQEPPEARGAVRDRWESLNALMQLAEAAVP
                     GTTLKQFVDELFERQAGQHEPVLSAVTLATLHSAKGLEWESVHLVGLSEGLLPISYAK
                     DAAAVEEERRLLYVGVTRARRRLGLSWSASPAPGRSAQRQPSRFLSDL"
     gene            complement(2969..3874)
                     /locus_tag="PLW15_08415"
     CDS             complement(2969..3874)
                     /locus_tag="PLW15_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34455.1"
                     /translation="MILKLDPRYPVVWRSPSSVQVGIDPPIVVVEDLTSGQERLLAAL
                     GPGVSDAGVGIVTREAPAQARELLDALAPALLPAAESATQPLVVALSGSGPLADDLAR
                     ILAGSGVHVLTSPRVEALVDEGADLAVAVAHYVLAPELHSVWLRRDVPHLPIVFSDSA
                     VRVGPIVEPGAGPCLRCLELHHRDRDIAWPAIATQLVGRRGAAESALLAGEGAAAAAR
                     LVLDRLSLDRLSLDRLAGGSRASSVSLRIDADTGERSYATWRQHPDCGCRGIEHLGAL
                     GPRGRSGIDWAAAARSGRGLGSPTS"
     gene            complement(3927..5273)
                     /locus_tag="PLW15_08420"
     CDS             complement(3927..5273)
                     /locus_tag="PLW15_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOT34456.1"
                     /translation="MPDDSPEDDRDNPEDEFRDMLRDFLAGNTSIDPAKLAGAAGLPN
                     DPEMVAQLISQLQSALANSGDGINWGLALEQARGIAGRTTVVSLPAERSQLEQALHVA
                     SLWLDEVTEISPLATEPRLMSRQEWVAATMPVWTQLAEPVANSIANSLTEVLQQSAGD
                     ELDDMIKGASQMMRNVGGTLFAMQLGQVVGQLSSEVVSGGDIGIPLMESDQAALIPQN
                     TIAFGAGLDIPIDQVELYLAVRELAHARLFKHAKWLRLQLLTSITEFARGIHIDTERL
                     EQLASDFDPNNPEELREAMTSGALIPPKSPEQLAALERLETMLALIEGWVDVVTAQAT
                     TRLPSSAAIAETVRRRRASGGPAESAFATLVGLELRPRRLREAAAMWQQVTDAVGNSA
                     RDELWSHPDLVPQGEDIDDPSALIARITATEVQPDDIDRAIEDLLGDDTTDRPHED"
BASE COUNT          800 a         1864 c         1930 g          850 t
ORIGIN      
        1 ccgacggatg ccgcgagcga atccggtccg gcggtgtacg agtcgagcgg ggtcttcgtg
       61 ccgtagacga actcatagac gatgccgcgg gtgctgtcga cgggcacctg gccggcgaag
      121 gcggagacgg cgaacggcag tcgggtgcgc acgccggtgc tcagtgcccg aagggccacg
      181 aggtcggcgg actgctccgc ctcggcccgc ggattgcgcg gaacccggat gatccagtgc
      241 cgaccgtctc tgccggtgat gatcgccgcg tcgaagtcgc tgccctgtcc cgccccgaaa
      301 ctggcggcgg cggcgatgtc gagacccggg acggcggatg tcgcgagcgc ggctagagtg
      361 agatgagatc tggccatacc gctaagggta ggtcggctac gcctcgcatc cgccgcacgc
      421 cacgcatctt cacggggtcg catccatgtc acagtccttc ctctcgaggc tcccgctgtc
      481 gcgtttcgag ctggatcggg accacctggg ccgcgaacgc ccggccctgt tcgacgagtt
      541 gtgggcgaac ccggccacgc gcatcctgcc gatcttccag ggttcggcgc tgctggcgac
      601 gcccgattcg ctcgcgctgc tgccggtgga tgctgtgccg cacttcagcg aacgggtcta
      661 cctcggtacg tcaacgtcga cgacgagtcc cgagcccgcg ggaaccccga tcgtggccgt
      721 cgagttggac gatgctggcc accttgccga gtcggactgg gcgaacctgc gccaggtcgg
      781 cagccgcctg agcgaccgcg acgcagggct cctcactgag gccctgggca tcctgaactg
      841 gcacacctcg caccgcttct cgccgcgcac cggcgaaccg actgtgtcgg agaagggcgg
      901 ctgggtgcgc cgcgacccgg tgtcgggcgg cgaggtcttc ccgcgcaccg accccgcgat
      961 catcgtgggc gtcaccgacg ccgacgaccg catcctgctc gggtcgaacg ctctctggga
     1021 gagcaaccgc tactcgctgc tcgcaggctt cgtcgagccg ggggagtcgc tcgagagcgc
     1081 cgtcatgcgc gaggtgcacg aggagtcggg cctgcgcgtc gtcgacccgg tgtacctggg
     1141 cagccagccg tggccgttcc ccgcgtccct gatggtgggt ttcaccgcgc gcgtggagga
     1201 cggcttcacg ggtgccgcaa cgcccgatgg cactgagatc ctcgacctgc ggtggttctc
     1261 ccgcgccgag ctggccatgg ccctcgacga gatccgtctg ccaggaccga cgtcgattgc
     1321 ccgggcgata gtggagcact ggtacggctc gagcatcgac gacggccagc ggtgaccaca
     1381 cccgcagccc tgctcgacgg actcgacgac cagcagcggg tcgcggccga agcgctgctg
     1441 ggaccggtct gccttctcgc cggtgcgggc acgggcaaga cgcgcgcgat cacccaccgc
     1501 atcgcctacg gggtcgcgac cggcgtctat ccgcccgggc gggtgatggc tctcaccttc
     1561 accgcgcgcg ccgcaggcga acttcgggga cgactgcgcg ccctcggtgc cggcggggtc
     1621 gcggcccgca cgttccacgc atccgcgctg gcgcaactga acttcttctg gccccaggtg
     1681 atcggcggca cgatgccgcg gctcctcgac agcaaggcgc gcctgatcgc ccacgccgcc
     1741 gacggcctca agctcaaact cgacacggcg acgctgcggg atgccgcggc cgaggtcgaa
     1801 tggcgcaaga cctcgcgtct gagcgtggag cagtacgcca cccgaactcg cacgttgcct
     1861 acttcgctgc ccatggatcg catggtcgat cttcagcgtg cctacgagga cctcaaggac
     1921 gagcgccgtc agctcgactt cgaggatgtg ctcctggccg cggccggaat gatcgaagcg
     1981 gagccgcggg tggcgcagca ggtgcgcgag cagtaccggt tcttcgtggt cgacgagtat
     2041 caggacgtgt caccgctgca gcacgacctg ctcgaactct ggctcggtga ccacgacgac
     2101 ctgtgcgtgg tgggcgacgc cagccagacg atctactcgt tcgcgggggc gagtcccgag
     2161 tacctgctcg gcttcgcggg cgagcacgag aatgccgtgg tgctgcggct ggagcagaac
     2221 taccggtcgg ctccgccgat catcgatgcg gcgaaccgtc tcatgcgcgg tcgtccgggt
     2281 gcgctcagtc tgaccgccgc atccgagctg accggccctc ggccgaccgt gaatacctac
     2341 gacagcgaca gctcggaggc gcgggcggtg gcggcggcca tcgccgagga gatcgcctcg
     2401 ggcatcccgc cggagcagat cgccgttctg tatcgcatca atgtgcagtc gcaggcgatc
     2461 gagacggcgc tgggcgatgc cggagtgccc tgcgtggtgc gcggtgcgac gcgcttcttc
     2521 gaccagcgcg aggtgaagga ggcgctcatg ctgttgcgcg gcgcgaccgt cacgacctcg
     2581 atcgagccgc tgttcaagac cgtcagcgac gtgctgcgct cgatcggctg gtcgcaggaa
     2641 ccgcccgagg cccgcggagc cgtgcgggat cggtgggagt cactcaacgc cctcatgcag
     2701 ctcgccgagg cggccgtgcc gggcacgacg ctcaagcagt tcgtcgacga gctgttcgag
     2761 cgacaggcgg ggcaacatga gccggtcctg tccgccgtca ccctggccac cctgcattcg
     2821 gccaagggcc tcgagtggga gagtgtgcac ctcgtcgggc tcagcgaggg tctgctgccg
     2881 atcagctacg cgaaggatgc cgccgccgtc gaggaggaac gccgactgct gtacgtgggg
     2941 gtcactcgcg cccgccgtcg gctcggactc agctggtcgg cgagcccagc ccccggccgc
     3001 tccgcgcagc ggcagcccag tcgattcctg agcgacctct agggccgagc gcaccgaggt
     3061 gctcgatgcc gcggcatccg cagtcggggt gctggcgcca ggttgcgtag gagcgctcgc
     3121 cggtgtccgc gtcgatgcgc agcgacacgg atgacgcgcg cgaacccccg gccaaccggt
     3181 cgaggctcag ccggtcgagg ctcagtcgat cgagtacgag gcgcgccgcc gccgcagcgc
     3241 cctcgcccgc gagcagggcg gactccgcgg ctccccgtcg ccccaccagc tgggtcgcga
     3301 tggcgggcca cgcgatgtcg cggtcgcggt ggtgcagctc caggcagcgc aggcagggcc
     3361 cggcccccgg ctcgacgatg gggccgaccc tcacggcgga gtcgctgaac acgatcggca
     3421 ggtggggaac gtcacgccgc aaccacaccg agtggagttc gggcgccaac acgtagtggg
     3481 cgacggcgac ggccaggtcg gctccctcgt cgacgagtgc ctccacccgt gggctggtga
     3541 gcacgtgcac accgcttccc gcgagaatcc gcgcgaggtc atcggccagc gggccggagc
     3601 cggacagggc aaccacgagt ggttgggtgg cggactcggc agcaggcagc agcgccggag
     3661 ccagggcgtc gagcagctcc cgggcctgcg ctggcgcctc cctggtgacg atgccgacgc
     3721 ccgcgtcgct cactccgggg ccgagggcag cgagtagccg ctcctgaccg ctggtgagat
     3781 cctcgacgac cacgatcggc ggatcgatgc cgacctgcac gctcgacggg cttcgccaga
     3841 cgaccggata ccgagggtcg agcttcagga tcatgcgctc aagggtgccg gttcaccgcc
     3901 tcgccgcgag ccagcgttcc acaggctcag tcctcgtgcg ggcggtccgt cgtgtcgtcg
     3961 ccgagtagat cctcgatcgc ccggtcgatg tcgtcgggct gcacctcggt ggcggtgatg
     4021 cgcgcgatga gggcgctcgg gtcgtcgatg tcctcaccct ggggtacgag gtcggggtgc
     4081 gaccacagtt cgtcgcgcgc ggaattgccg acggcatccg tcacctgctg ccacatcgct
     4141 gcggcctcac gcaggcgacg cggacgcagc tcgaggccca cgagggtggc gaatgcggat
     4201 tctgccgggc cgcccgaggc gcgccgccgc cgtacggtct cggcgatcgc ggcgctcgac
     4261 ggaaggcgcg tggtggcctg tgcggtgacc acgtcgaccc agccctcgat gagggcgagc
     4321 atcgtctcca gccgctcgag cgcagcgagc tgctcggggg acttcggcgg gatcagcgcg
     4381 ccgctggtca tggcctcgcg cagttcctcg ggattgttcg gatcgaagtc gctcgcgagc
     4441 tgctcgaggc gttcggtgtc gatgtggatg ccccgggcga actcggtgat cgaggtgagc
     4501 aactgcaggc gcagccactt ggcgtgcttg aacagccgcg cgtgggcgag ctcacgcacc
     4561 gccaggtaca actcgacctg gtcgatggga atgtcgaggc cggccccgaa cgcgatcgtg
     4621 ttctggggga tgagcgcggc ctggtcgctc tccatgagcg ggatgccgat atcgcctccc
     4681 gagacgacct cgctcgagag ctggccgacg acctgcccga gttgcatcgc gaacagggtg
     4741 ccgccgacgt tgcgcatcat ctggcttgcg cccttgatca tgtcgtcgag ctcgtcgccg
     4801 gcgctctgct ggagcacctc ggtgagcgag ttcgcaatcg agttggccac gggctcggcg
     4861 agctgggtcc acaccggcat cgttgcggcc acccactcct gccggctcat caggcgaggt
     4921 tcggtcgcaa ggggtgagat ctcggtcacc tcatcgagcc agagggatgc cacgtgcagg
     4981 gcctgctcga gctgcgaacg ctcggcgggc agcgacacga ccgtggtgcg tccggcaatg
     5041 ccgcgcgcct gttcgagcgc gagaccccag ttgattccgt cgccgctgtt ggccagcgcg
     5101 ctctgcaact ggctgatcag ctgggcgacc atctcgggat cgttgggcag tccggctgca
     5161 cccgcgagct tggccgggtc gatgctcgtg tttcccgcga ggaagtcgcg gagcatgtcg
     5221 cggaactcgt cctcggggtt gtcccggtcg tcttccggcg aatcgtcagg catgaaaact
     5281 acggtagccg ctgcgtggtc ggcgatgctg acttttcacc taggctgggg ctcgtggctg
     5341 tgcgcctgtg gcgaacagat gggcagcccc acccgacgcc tctccagacc ccggaggatc
     5401 gctctgaccc tgttcaccga tctcgaccct gatccgccag cacg
//
LOCUS       DAOSOU010000148         5438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15138_length_5438_cov_5.261007, whole genome shotgun sequence.
ACCESSION   DAOSOU010000148 DAOSOU010000000
VERSION     DAOSOU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15138_length_5438_cov_5.261007"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLW15_08425"
     CDS             <1..397
                     /locus_tag="PLW15_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HOT34457.1"
                     /translation="APAPKPTPSNTPKAAVTPTATPTPTPTPTQAPAPVETVSQANAK
                     RKAVDYLNYTAFSRSGLIDQLVYEGFTPEDSAYGVDSLGVDWSAQAVAKAQQYLDYDA
                     FSHSGLVDQLVYEGFTADEAEFGVTAVGL"
     gene            complement(467..1354)
                     /locus_tag="PLW15_08430"
     CDS             complement(467..1354)
                     /locus_tag="PLW15_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34458.1"
                     /translation="MMNEDEWPNGVSRGPWSPDRESRDAGRRVHFEILPHPDLKLFPM
                     GITLGSFDTEGETYADALTDMARLIEEFRFETAVAQMYSHGVKREVRELRARMEEHPH
                     SLGFSNRARAMLVSYTSAGVDPSELIDNPQMPIAGTRVLGGWWAAQLYDSAMMRGTAI
                     LDRLVTLLFCIEGRPVDPNWMPVFRHRTLRQLVTWAGEPEWKVLLDTLDHELFELAKG
                     YRDGLVHRHRYSAELHGDHLVGRWEEGAWWNELGIKSESHFALVAAFYNEVLLPAIQA
                     TGDLIARTMEERGVERASD"
     gene            complement(1512..2150)
                     /locus_tag="PLW15_08435"
     CDS             complement(1512..2150)
                     /locus_tag="PLW15_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOT34459.1"
                     /translation="MIPLANLIAFVLVAIPIILVPGPSVLFVIGRSLSLGRIGGLLSV
                     VGNAIGGLVVVVAVAFGVGILIAQSLVVFTIVKIAGAGYLIYLGIQAIRHRRQHADAA
                     TATPPARSKLRILTQGFLVGVSNPKTIVFFIAVLPQFVDYGAGGIPLQMLLLGTLFIV
                     IALLTDSVWALVAGVARDWFARSPRRIEALGATGGVVMIGLGVGSLFVGNRH"
     gene            complement(2176..2433)
                     /locus_tag="PLW15_08440"
     CDS             complement(2176..2433)
                     /locus_tag="PLW15_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HOT34460.1"
                     /translation="MFALLPLDANDNGTNFALIGIIVWVIVVIAQAVLFIASLISILG
                     SKRYTGGGKFLWLVVVFFAPFLGAIGWFIAGRTAQIRTSAP"
     gene            complement(2479..3759)
                     /locus_tag="PLW15_08445"
     CDS             complement(2479..3759)
                     /locus_tag="PLW15_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34461.1"
                     /translation="MSTVGEPFRWRSIALPALLPTILFSIGEGAIIPIIPTVADDLGA
                     TLAIAGLVAAVLTIGELLGNIPSGWLIARIGERPAMIGATSLSVVGLVICLIAPNPLA
                     LGFGIFLVGLSAAVFALARHAFMTSFVPVAYRARALSTLGGTFRLGYFVGPFITAGVI
                     QLTGTAGAAFWIHLVACAGAALVLVTLPDPTTTFGKVATVRLEGRQMREGEALVARES
                     SGLFRTLWASRGILSRLGTGAALVGALRASRQVILPLWAVSIGVSATNTALIIGIAGA
                     VDFALFYFGGWIMDRYGRLASALPSMIGLGAGHLLLSFTHDLPSNVAWFIAAAMVLSV
                     ANGLGSGILMTLGSDLADKRNPAPFLGAWRFTAGVGGAAAPLLIAGITAIASLAPAAA
                     VMGVLGMLGAGVLARYVPRYIPRPPRAPRPGPNR"
     gene            3820..4923
                     /gene="prfB"
                     /locus_tag="PLW15_08450"
     CDS             3820..4923
                     /gene="prfB"
                     /locus_tag="PLW15_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT34462.1"
                     /translation="MIELDISDQLAALRSTFADIRAVVGVEKLEAEIADLSEQAGAPD
                     LWDDTDKAQKVTSALSHRQAELAKIVSIGSRLDDIEIMVELANEESDESAAADVHTEL
                     ASIQKLLGELEVQTLLDGEYDDRPAVITIRAGAGGVDAADFADMLMRMYLRWAEKHKY
                     TATVMDVSYAEEAGIKSATFEIDAPYAFGTLSVEAGTHRLVRMSPFGAAGKRQTSFAA
                     VEVIPLMPETEVIDIPEGDIRVDVFRSSGPGGQSVNTTDSAVRLTHIPTGTVVSMQNE
                     KSQIQNRAAALRVLQSRLLILQKEQEAAVKKDLAGVITASWGDQMRSYVLAPYQMVKD
                     LRTEYEEGNPSKVFDGDIDSFIAAGIKWRKRDK"
     gene            4902..>5438
                     /locus_tag="PLW15_08455"
     CDS             4902..>5438
                     /locus_tag="PLW15_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOT34463.1"
                     /translation="MAQARQIVPGTPEVERRALAWSVVRVVATGVAMLVFYFVLPFRD
                     VSAADTLLRLVGGIVLIAVAIGWQVRAISRSHQPLLRSVEAVGLSFWLLVIVFSIVYV
                     SMSASNPLAFSEPLNEVGGLYFTMSILATVGFGDISAVSDAARIVVIVQIVLDLLLLG
                     VVVRLLLGAGKAAAEARKT"
BASE COUNT         1011 a         1833 c         1766 g          828 t
ORIGIN      
        1 cgcgccagcc ccgaagccaa ccccgagcaa cacaccgaag gctgccgtta cacccacggc
       61 aacgccgaca cccaccccaa caccgactca agcgccggcc cccgtagaaa ccgtgagcca
      121 ggccaacgcc aagcggaagg cagtggacta cctgaactac acagccttct cgcgtagcgg
      181 tctcatcgac cagctcgtgt acgaagggtt cacacccgaa gactccgcgt acggcgttga
      241 cagcctaggt gtcgactgga gtgctcaggc cgtggccaag gcccagcagt acctggacta
      301 cgacgctttc tcgcacagcg gtctggtaga ccaactggtc tatgaagggt tcaccgccga
      361 cgaggcggaa ttcggggtca ctgctgtcgg gctgtagcgc gcgtttcacg tagcgatcgc
      421 gtggtcaaac cttcgagcct ggggagttcg cgtcgtgagc aacgccttag tccgacgcgc
      481 gctcgacccc tcgttcctcc atcgttcgtg cgatcaaatc cccggtggct tgaatggcag
      541 ggagtagaac ttcgttgtag aacgcagcta cgagcgcgaa gtggctctcc gacttaatac
      601 ccagctcatt ccaccatgcc ccttcctccc agcgacctac caagtggtcg ccatgaagtt
      661 cggccgagta gcggtgtcga tgtacaaggc cgtctctgta ccccttcgcc agctcgaata
      721 gctcgtgatc aagagtatcg agtaggacct tccattcggg ttcccccgcc caagttacta
      781 actgcctgag ggtcctatgc cggaatacgg gcatccagtt cggatcgacc ggtcgacctt
      841 caatacagaa aagcaatgtc acaaggcggt caaggatcgc agtgccgcgc atcattgcgg
      901 agtcataaag ctgtgccgcc caccatcccc cgagaacacg ggtgccagcg atcggcattt
      961 gagggttgtc tatcaactca gatgggtcaa ctcccgcaga ggtatagctc accagcatcg
     1021 ctcgagctct attggaaaac cccaagctat gtgggtgctc ctccattctc gccctcagtt
     1081 cgcgtacctc gcgcttcacc ccatgtgagt acatttgcgc cacagccgtc tcaaatcgaa
     1141 actcctcgat gagccgcgcc atatccgtca gggcgtccgc gtaggtctcg ccctcagtat
     1201 caaaggaacc caatgtaatg cccatgggga agagcttcag atcggggtgg ggcagtatct
     1261 caaagtgaac ccggcgaccc gcgtcgcggc tctcacggtc agggctccag ggccctctcg
     1321 agacaccatt gggccattcg tcctcgttca tcacggcggt cacactatcc gttcgcacgc
     1381 cgatgccgtt tgaagcgcct gcgcccgttc cggtgaggat tttacggtca ccgcgccgcc
     1441 agctcggtgc ccgcgcggct gctggaaccg ccgcgcgccc aacgaacccc gcgcggtcgc
     1501 tcggctccta gctagtgccg gttccccacg aacagcgacc ccacacccag cccgatcatc
     1561 accacgccac cggtcgcgcc gagcgcctcg atgcgccgcg gggagcgggc gaaccagtcg
     1621 cgcgcgaccc ccgccacaag cgcccagacg ctgtcggtca gcagcgcgat cacgatgaac
     1681 agggtgccga gcagcagcat ctgcagcggg atgccgccgg ccccgtagtc gacgaactgc
     1741 ggcagcacgg cgatgaagaa cacgattgtc ttggggttcg agacaccgac caggaacccc
     1801 tgcgtgagga tgcgcagctt gctgcgggcg ggcggcgtcg cggtcgcggc gtccgcgtgc
     1861 tgacgacggt gccggatcgc ctggatgccc agatagatca ggtagccggc gccagcgatc
     1921 ttcacgatcg tgaacacgac gagggactgc gcgatgagga tgccgacccc gaaggccacg
     1981 gcgaccacca cgacgagtcc gccgatcgcg ttaccgacga cggagagcag gccgccgatg
     2041 cgaccgagcg acagcgagcg gccgatcacg aacagcacgc tcggaccggg cacgaggatg
     2101 atcgggatcg ccacgagcac gaaggctatg aggttggcga gcgggatcat cccgcaatgc
     2161 tagacccgga acgggctacg gggcagaggt gcggatctgc gcggtgcgtc cggcgatgaa
     2221 ccagccgatc gcgccgagga agggcgcgaa gaacacgacg accagccaga ggaacttgcc
     2281 gccgccggtg tagcgcttcg agccgaggat cgagatcagc gaggcgatga acagcaccgc
     2341 ctgcgcgatg acgacgatga cccagacgat gatgccgatg agggcgaagt tggtgccgtt
     2401 gtcgttggcg tcgaggggca gaagcgcgaa catggtgtcc tttccgttcc ccgacgggac
     2461 ggggtttcgg cgctcagact accggttcgg acccggtcgt ggtgctcgcg gcgggcgggg
     2521 gatgtagcgc ggcacatagc gcgcgagaac gccggcgccg agcatcccga gcacccccat
     2581 gacggccgcg gcaggggcga gcgaggcgat ggcggtgatg cccgcgatga gcaacggggc
     2641 cgccgcgccg ccgacccccg cggtgaaccg ccacgcgccc aggaacggcg ccgggttgcg
     2701 cttgtccgcg aggtcggacc ccaacgtcat caggatgccg ctgcccagcc cattggccac
     2761 cgacagcacc atcgccgccg cgatgaacca tgccacgttg ctcggcaggt catgggtgaa
     2821 cgagagcagc aggtgcccgg cacccagccc gatcatcgac ggcagcgccg aggcgagacg
     2881 gccatagcgg tccatgatcc acccgccgaa gtagaacagc gcgaagtcga cagcgccggc
     2941 gatgccgatg atgagtgcgg tgttcgtggc gctcacgccg atgctcaccg cccacagcgg
     3001 caggatcacc tggcgactcg cccgcagcgc cccgacgagt gcggcgcccg ttcccagccg
     3061 gctcaggatg ccccggctag cccacagcgt gcggaacaac ccgctcgact cccgtgccac
     3121 caacgcctca ccctcgcgca tctgccgccc ctcgaggcgc accgtggcga ccttgccgaa
     3181 cgtcgtcgtc ggatcgggca gcgtcaccag caccagggca gcgccggcgc aggcgacgag
     3241 gtgaatccag aatgccgccc ccgccgtgcc ggtgagctgg atgacgccgg ccgtgatgaa
     3301 cggccccacg aagtagccca gcctgaaggt gccgcccagc gtcgacagcg cgcgggcgcg
     3361 ataggccacc ggcacgaagc tcgtcatgaa cgcgtgacgc gcgagcgcga agaccgccgc
     3421 cgacagcccc acgaggaaga tgccgaagcc gagcgcgagc gggttgggcg cgatcaggca
     3481 gatcaccagc ccgaccaccg agaggctggt cgcgccgatc atggccgggc gctcgccgat
     3541 gcgggcgatg agccatccgc tcgggatgtt tcccagcagc tcgccgatcg tgagcacggc
     3601 cgcgacgagg cccgcgatcg cgagggtggc gccgaggtcg tcggcgaccg tggggatgat
     3661 cgggatgatc gcgccctcgc cgatcgagaa caggatcgtc ggcagtaggg cggggagggc
     3721 gatcgagcgc cagcggaacg gttcaccgac tgtcgacatc tctgcgatgc tacccgcgcc
     3781 cggcccccgg cggggcgaga cggtagtctg gtctgcgaca tgatcgagct cgatatctcc
     3841 gaccagctgg ccgccctgcg ctcgaccttc gccgacattc gcgctgttgt gggcgtggag
     3901 aagctcgagg ccgagatcgc cgacctcagc gagcaggcgg gcgcacccga cctgtgggac
     3961 gacaccgaca aggcgcagaa ggtcacgagc gcactgagcc accggcaggc cgagctcgcc
     4021 aagatcgtct cgatcggctc gcgcctggac gacatcgaga tcatggtcga gctcgcgaac
     4081 gaggaatccg acgagtccgc cgccgccgac gttcacaccg agctggcgag catccagaag
     4141 ctgctcggcg agctcgaagt gcagaccctg ctcgacgggg agtacgacga tcgcccggcg
     4201 gtcatcacaa tccgcgccgg tgccggtggg gtggatgccg cggacttcgc cgacatgctc
     4261 atgcgcatgt acctccgctg ggccgagaag cacaagtaca ccgccacggt catggacgtc
     4321 agctacgcgg aggaggcggg catcaaatcc gccaccttcg agatcgacgc cccctacgca
     4381 ttcggcacgc tcagcgtcga agcgggcacc caccgtctcg tgcggatgag ccccttcgga
     4441 gcggcgggca agcgccagac gtcattcgcc gcggtcgagg tcatcccgct catgccggag
     4501 accgaggtga tcgacatccc cgagggtgac atccgggtgg acgtcttccg ctcgagcggg
     4561 cccggcgggc agtcggtcaa cacgaccgac tcggccgtgc ggcttaccca cattccgacg
     4621 ggcaccgttg tctcgatgca gaacgagaag agccagatcc agaaccgtgc tgccgcactt
     4681 cgggtgctcc agtcgcgact gctcatcctc cagaaggagc aggaggcggc ggtcaagaag
     4741 gatctcgccg gagtgatcac ggccagctgg ggcgaccaga tgcgctccta cgtgctcgcg
     4801 ccgtaccaga tggtcaaaga cctgcgcacc gagtacgaag aaggcaaccc ctcgaaggtg
     4861 ttcgacggcg acatcgacag tttcatcgct gcgggtatca agtggcgcaa gcgcgacaaa
     4921 tagtcccggg aacccccgag gtcgagcgca gggccctcgc ctggtctgtg gtgcgggtgg
     4981 tcgccaccgg cgtcgcgatg ctggtcttct acttcgtgct gccgttccgc gacgtcagtg
     5041 cggccgacac gctgctgcgt ctcgtcggtg gcatcgtgct catcgcggtt gcgatcgggt
     5101 ggcaggtgcg cgcgatttct cgctcccacc aaccgctact gcgctccgtc gaggcggtgg
     5161 ggctctcgtt ctggctgctc gtcatcgtct tctcgatcgt ctacgtgtcg atgtcggcgt
     5221 cgaacccgct ggcgttcagc gaaccgctca acgaggtcgg cggcctgtat ttcacgatgt
     5281 cgattctcgc gacagtgggc ttcggcgaca tctccgcagt ctcggatgcc gcgcgcatcg
     5341 tcgtcatcgt gcagatcgtg ctcgacctgc tcctcctcgg cgtggtggtg cgcctgctgc
     5401 tcggcgcggg caaggccgcg gccgaggcgc gcaagacg
//
LOCUS       DAOSOU010000149         5421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15200_length_5421_cov_5.975960, whole genome shotgun sequence.
ACCESSION   DAOSOU010000149 DAOSOU010000000
VERSION     DAOSOU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15200_length_5421_cov_5.975960"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PLW15_08460"
     CDS             <1..1388
                     /locus_tag="PLW15_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT34464.1"
                     /translation="ALLIGDHDVIFLDEPTNHLDVEGIAWLAAHLKRRWSTNSGGLVV
                     VTHDRWFLDEICTATWEVHDRLIEPFEGGYAAYILQRVERDKMAATMESKRQNLMKKE
                     LAWLRRGAPARTAKPKFRIDAANELIANEPPVRDSVALSQMAMQRLGKDVVDLENVTV
                     SYGDKTVLKDVTWRIAPGERTGILGVNGAGKSTLLSLVSGSLLPTTGRVKRGKTIKVA
                     TLTQQLFELEDIWNDRVSEVIGRQKSTYVTGGKEMTPGQLLERVGFSSAQLSTPVKDL
                     SGGQKRRLQLLLILLSEPNVLILDEPTNDLDTDMLAAIEDLLDSFPGTLLVVSHDRYL
                     VERVTDQQYAVMNGALRHLPRGVDQYLELRASGGLSSPKGVPKPGAVSTGSTSLSGAE
                     LRNAQKEHAAASRKIEKLNGQIAGVHERMAEHDQSDYTGLGALAAELAGYQEQLDTVE
                     LRWLELEDLLS"
     gene            1417..1614
                     /locus_tag="PLW15_08465"
     CDS             1417..1614
                     /locus_tag="PLW15_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34465.1"
                     /translation="MANYDRGRAATSGGVGFFFLLAYIGAAIYFISHSDGSFWGVILG
                     LLQAIVWPVYVIYHVLTALGA"
     gene            1649..2656
                     /locus_tag="PLW15_08470"
     CDS             1649..2656
                     /locus_tag="PLW15_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOT34466.1"
                     /translation="MHPASTRIAGLGVYLPETTRSAAQTERDLRAANPRLKIATGLIH
                     RLTGVDTVHLRAHGWQASDLAVEAARIALQESPGDPDLLIFASASQDLIEPATSHIVA
                     AKLGLTCPVMDISNACNSVINAMQVGEALIATGQYRRVLIASGEQPSHGVRWQLRDQE
                     QFIRSFPGYTMSDGGAAVVLEATDDPATGILDSSFVAVSRHWQIGTLPAGGTMNPRSV
                     DHTYFDMDGQGLQQAFLELGPAPVLDLLERNGLSWDDFDLVAIHQVALPYLPPIFERL
                     GVAPDKSVITIADHGNLASVTLPLQLSIARETGLVGPGSLVLLIGLAGGISLGLMVVR
                     L"
     gene            2653..3450
                     /locus_tag="PLW15_08475"
     CDS             2653..3450
                     /locus_tag="PLW15_08475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34467.1"
                     /translation="MTPAGLDTPPSEALDRPGLAVVVPVFNEAAGITPTLEALAAQRD
                     SDFDVVFVDNGSTDASADVIREFAASRALTRWRVIAEAQKGTGAAADTGMRAAIAAGA
                     EYLARTDADCLPREDWTGSIRRALDSGLGLVGGELVPRHDEGLTWARRVALRAAVEVA
                     EAFGRIRPGNRSPEYRGPYMMAAGCNVAITAELYLAAGGFPRTAIEDLHEDRALVNAV
                     RRLTNRYARRRDVVVFGSSRRVNAWGLKNTLLWYKDHAYRPEHVDIR"
     gene            3495..4637
                     /locus_tag="PLW15_08480"
     CDS             3495..4637
                     /locus_tag="PLW15_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT34468.1"
                     /translation="MRAAHPVAYPALGLLRAPARRVPGLGVLVRDAALLRATLMDTEH
                     FSKTGPGAPSDLWTPVLGPAVLLNMEGADHLALRRKLGPLFAPAYVDELVAASLPAEQ
                     LTARLVAEERVDLVAEAKRNASIMISQLVGLDSAHVNEELFDRVSAITRFVRLSRPRL
                     SDAQVGVARGILDELTAHARTAYRAGDEATVPGRMRALGLGEDEAMGAVGAFVLTGTE
                     TLVSYVPRLVALLIDSGWMPRLATDPALIEPAIAEGLRVTTPSPVMLRSVVADATVGS
                     VPVRVGDRVILATFLANRAGGGFDPEANPAASLKQLWFGAGTHFCLGAPLAMAQVRVT
                     LGALLAAGPLEIVARRAARGVLIPSYARLELSRLSSPQGVSKPAAP"
     gene            4634..>5421
                     /locus_tag="PLW15_08485"
     CDS             4634..>5421
                     /locus_tag="PLW15_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT34469.1"
                     /translation="MTDILAALLDSCERTPDAPALSLRRTTLRYRDLADRIHGVASSL
                     TAAGLKPGDRVLFSVRPGIDGICLALGIIAAGGTVVFADPGAGDAMFRARAALAAPAW
                     VAAESVLYAASSGPLRGIARRRGLELPDYGTVVPDARHIYAGPWLPGVPRGALRFSRL
                     SSALELGAKAISGLRRDASADGRPGRLITGERSRYAAAPLLDQPGTASPLLDQPNHEA
                     LIIFTSGTTDAPKAVVHSRASLGTGLDDFASAIGFVPGELVLTD"
BASE COUNT          792 a         1926 c         1860 g          843 t
ORIGIN      
        1 ccgcgctgct catcggcgac cacgacgtga tcttcctcga cgagcccacg aaccatctgg
       61 acgtcgaggg catcgcctgg ctcgccgccc acctcaagcg ccgctggtcg acgaactcgg
      121 gcgggctcgt ggtcgtgacc catgaccggt ggttcctcga cgagatctgc accgccacgt
      181 gggaggtgca cgaccggctc atcgaaccct tcgagggtgg gtacgcggcg tacatcctgc
      241 agcgggtcga gcgcgacaag atggccgcca ccatggagtc caagcgccag aacctcatga
      301 agaaggagtt ggcgtggctg cgccgagggg cgcccgcacg cacggccaag cccaagttcc
      361 gcatcgacgc ggccaacgag ctcatcgcca acgagccgcc ggtgcgcgac agcgtcgccc
      421 tctcccagat ggccatgcag cgcctcggca aggacgtcgt cgacctcgag aacgtcaccg
      481 tctcctacgg cgacaagacc gttctgaagg atgtcacgtg gcgcatcgcc ccgggcgagc
      541 gcacgggcat cctcggggtg aacggcgcag gcaagtcgac gctgctctcg ctggtctccg
      601 gatcgctgct gccgaccacc gggcgggtca agcgcggcaa gacgatcaag gtcgcgaccc
      661 tcacccagca gctgttcgag ctcgaggaca tctggaacga tcgcgtcagc gaggtcatcg
      721 gtcgccagaa gagcacctac gtcaccggcg gcaaggagat gaccccgggc cagctgctcg
      781 agcgggtcgg gttctccagc gctcaactgt cgactcccgt caaggacctg tcgggtggcc
      841 agaagcgtcg gctgcagctg ctgctgattc tgctcagcga gcccaacgtg ctgattctgg
      901 atgagccgac caacgacctc gacaccgaca tgctcgcggc catcgaggac ctgctcgact
      961 cgttccccgg cacgctgctc gtggtcagcc acgaccggta tctcgtcgag cgcgtcaccg
     1021 atcagcagta cgcggtgatg aatggcgcgc tgcgacacct gccgcgcggg gttgaccagt
     1081 acctggagtt acgtgcgtcc ggcgggttga gtagcccgaa gggcgtaccg aaaccgggtg
     1141 cggtttcgac aggctcaacc agcctgtcgg gcgccgaact gcgcaacgcc cagaaggagc
     1201 atgccgcggc atcccgcaag atcgagaagc tcaacggtca gatcgccggc gtgcacgagc
     1261 ggatggccga gcacgaccag agcgactaca cgggcctcgg cgcgctcgcc gccgagctcg
     1321 ccggctacca ggagcagctg gacacagttg aattgcggtg gcttgagctg gaagaccttc
     1381 taagctgagg ctcacctgag cgtgaggagc atcaccatgg cgaactacga ccgcggccga
     1441 gccgccacca gcggtggggt ggggttcttc ttcctgcttg cctacatcgg tgcggccatc
     1501 tacttcatct cgcactccga cggcagcttc tggggtgtca tcctcgggct gcttcaggcg
     1561 atcgtctggc cggtgtatgt gatctaccac gtgctgactg ccctcggggc ctagcccagg
     1621 cgcacggcgt cgccactagg ctcgcagggt gcatcccgcg tccacccgaa tcgccgggct
     1681 cggcgtctac ctgcctgaga ccacccgctc ggcggcgcaa accgaacgcg acctgcgggc
     1741 ggcgaaccct cgcctcaaga tcgctaccgg gctcatccat cgcctcaccg gcgtcgacac
     1801 tgttcacctt cgtgcacacg ggtggcaggc gtcggatctc gccgtcgagg cagcgcgcat
     1861 cgcactccag gagtcgcccg gcgatcccga cctgctgatc ttcgcgagtg ccagccagga
     1921 cctcatcgag cccgcgacca gccacatcgt cgccgccaag ctcggcctga cctgcccggt
     1981 gatggacatc tccaacgcct gcaattcggt gatcaacgcc atgcaggtcg gtgaggcgtt
     2041 gatcgcgacc gggcagtacc gacgagtgct gatcgccagc ggcgagcagc cgagccacgg
     2101 ggtgcggtgg cagctgcgcg accaggagca gttcatccgc agctttccgg gctacacgat
     2161 gagcgacggc ggcgcggccg tcgtgctcga ggcgacggat gacccggcca cgggcatcct
     2221 cgattcgagc ttcgtggctg tctcccgcca ctggcagatc ggcacgctgc ccgcgggcgg
     2281 cacgatgaat ccgcgcagcg tcgaccacac ctacttcgac atggacggtc aggggctgca
     2341 gcaggcgttc ctcgagctgg ggcccgcgcc cgtgctcgac ctcctcgagc gcaacgggct
     2401 cagctgggac gacttcgacc tcgtcgccat tcaccaggtg gcgctgccgt accttccgcc
     2461 gatcttcgag cgcctcggtg tcgcacccga taagtcggtg atcacgatcg ccgaccacgg
     2521 caacctcgcc tccgtgaccc tgcctctgca gttgtcgatc gcgcgcgaga ccggactcgt
     2581 cgggccgggc agtctcgtgc tgctcatcgg gcttgccggt ggcatctcgc tcgggctgat
     2641 ggtggtgcgg ttgtgactcc ggcgggtctc gatacaccgc cttcggaggc actcgaccgg
     2701 ccggggctgg cggtcgtcgt gcccgtgttc aacgaggccg ccggcattac gcccacgctt
     2761 gaggccctcg ccgcccagcg cgactccgac ttcgacgttg tctttgtcga caacgggtcg
     2821 acggatgcct cggccgacgt gatccgtgag ttcgcggcat cccgtgccct gacccgctgg
     2881 cgagtgatcg ccgaggccca gaaggggacc ggggcggccg ccgacaccgg catgcgcgcc
     2941 gccatcgcgg cgggcgcgga gtacctcgct cgcaccgacg ccgactgcct gccgcgcgag
     3001 gactggacgg ggtcaatccg ccgggcgctc gactccggcc tggggctggt cgggggcgag
     3061 ttggttcccc gccacgacga agggctgacg tgggccaggc gagtggcgct gcgtgccgcc
     3121 gtcgaggtgg ctgaggcctt cgggcgcatc cggccgggaa atcgctcgcc cgagtaccgc
     3181 gggccgtaca tgatggcggc gggctgcaac gtggccatca ccgctgagct ctacctcgct
     3241 gcgggtggat tcccgcgcac cgcgatcgag gacctgcacg aggaccgtgc gctcgtcaac
     3301 gcggtgcggc ggctcacgaa ccgctacgcc cgccgccgcg atgtcgtggt cttcgggtcg
     3361 tcgcggcggg tcaacgcctg ggggctcaag aacaccctgc tctggtacaa ggaccacgcg
     3421 taccggcccg agcatgtgga tattcggtga ccgagcgccg ccgagatctg gccgcgcgct
     3481 gggagcagcg tgtcatgcga gccgcccacc ccgtggcgta cccggctctc ggtctgctgc
     3541 gcgcgccggc gcggcgcgtg cccggcctcg gtgtgctcgt gcgcgacgcg gccctgctgc
     3601 gcgcgaccct catggacacg gagcacttct ccaagaccgg cccgggtgcg ccgagcgacc
     3661 tgtggacccc cgtgctcggc ccggcggtgc tgctcaacat ggagggcgcc gaccacctcg
     3721 ctttgcggcg caagctcggg ccgctgttcg cgccggccta cgtcgacgag ctggttgcgg
     3781 catccctgcc ggctgagcag ctgaccgcgc gactcgtggc ggaggagcgg gtcgacctcg
     3841 tcgccgaggc gaagcgcaac gcgagcatca tgatcagcca gctggtgggg ctggattccg
     3901 cccacgtgaa cgaggagctg ttcgaccggg tcagcgcgat cacccggttc gtgcggctct
     3961 cgcggccccg gctcagcgac gcgcaggtgg gggtggcgcg gggcatcctc gacgagctca
     4021 ccgcccacgc ccgcacggcc taccgcgccg gcgacgaggc gaccgtgccc ggccggatgc
     4081 gcgcactcgg cctcggcgag gacgaagcga tgggtgccgt gggtgccttc gtgctcacgg
     4141 gcaccgagac cctcgtctcg tacgtgcccc ggctggttgc gctgctcatc gactcgggtt
     4201 ggatgccccg gctcgcgacc gaccccgcac tcatcgaacc cgccatcgcg gaggggctgc
     4261 gcgtgacgac cccgtcaccc gtgatgctgc gcagcgtcgt cgcggatgcc acggtcggct
     4321 ctgtgccggt gcgtgtgggt gaccgcgtga tcctcgccac cttcctcgcc aaccgcgccg
     4381 gtggcggatt cgacccggag gccaaccccg cggcctccct caagcagctg tggttcggcg
     4441 ccggcacgca cttctgcctc ggcgcgccgc tggccatggc gcaggtgcgg gtgacgctcg
     4501 gcgcgctgct ggcggccggg ccgctcgaga tcgtggcgcg gcgagcggcg cgcggggtgc
     4561 tcattccgag ctacgcacgg ctggagctca gccggttgag tagcccgcag ggcgtatcga
     4621 aacccgccgc gccgtgaccg acattctcgc cgcgctcctc gactcctgcg agcgcacccc
     4681 cgacgcgccc gccctgtcgt tgcggaggac gacgctccgc taccgcgacc tggccgaccg
     4741 catccacggc gtggcatcct cccttacggc ggcggggctg aagcccggcg accgcgtgct
     4801 gttcagcgtg cggcccggca tcgacggcat ctgcctcgcg ctgggcatca tcgcggccgg
     4861 cggcaccgtg gtgttcgcgg atccgggcgc gggcgatgcg atgttccggg cgcgtgcggc
     4921 gctcgccgcc cccgcctggg tcgcggccga gtcggtgctg tatgccgcgt catccggacc
     4981 gctgagggga atcgcgcgtc gccgcgggct cgagcttccc gactacggca cggtggttcc
     5041 ggatgctcgg cacatctacg ccgggccgtg gctgcccggg gtgccgcggg gcgcgctgcg
     5101 gttcagcagg ttgagtagcg cgctggagct aggagcgaag gccatttctg gccttcgccg
     5161 cgacgccagc gcagacggcc gtcccggccg tctgatcacc ggagagaggt ctcgatacgc
     5221 ggctgcgccg ctactcgacc agccggggac ggcttcgccg ctactcgacc agcccaacca
     5281 cgaggcgttg atcatcttca ccagcggcac caccgacgcg cccaaggcgg tcgtgcactc
     5341 tcgcgcatcg ctgggtaccg gtctcgacga cttcgcgagc gcgatcgggt tcgtgcccgg
     5401 cgaactggtg ctgaccgacc a
//
LOCUS       DAOSOU010000150         5392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15292_length_5392_cov_5.933296, whole genome shotgun sequence.
ACCESSION   DAOSOU010000150 DAOSOU010000000
VERSION     DAOSOU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15292_length_5392_cov_5.933296"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PLW15_08490"
     CDS             <1..1342
                     /locus_tag="PLW15_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOT34470.1"
                     /translation="DTKNGTVVTDVDYCYDWADRLTSTNATTTGGNPVLNTDLSTVGA
                     PATLAYDTHGNTTKLADQTLAYDVADRHMSTVLADGTTITYARDATGRVVSRITDAPG
                     TTNDSTIRYSFAGGSLFAVLDGSNQVLQRELSLPGGVSLSIPAAGGQVWAYPNLHGDV
                     ILTTDATGIRQGTRTSYDPFGQPLAANGDIGSTVADDSIPDTSPGDADYGYVGQHDKL
                     YEHQGSLATIEMGVRQYVPALGRFLSCDPVEGGVTNAYDYPSDSINSFDLSGRAQMGH
                     TMLDSSGSRGSPPRPEPEGHGRSGPTPLQRASDANQSTTQAAIQLALATGGTCSYVPN
                     QPMVFCTGITGIQLGAYTTIGNAVLTNGTHGPSGDVLTHEQYHMYQWAEMGAYRFTAA
                     WIGGELYSQRAQEQGWLPDNCRSERGCLNPIEIAAGPYIGNYWARPDAWPQGAR"
     gene            1379..1798
                     /locus_tag="PLW15_08495"
     CDS             1379..1798
                     /locus_tag="PLW15_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34471.1"
                     /translation="MLAIALSGCTSAPSLVSAKLDGDLVVFAVCEQIAYDQLTVLSDE
                     KGSIPSSFGETWVAKGLGATGGFVEIRYGVPPRGLETRVGPDALPTSGHYVHFELIGH
                     DDEGAIAGDLFAQFDGDKLVPERWLNSSGELRESPCR"
     gene            complement(1976..2057)
                     /locus_tag="PLW15_08500"
     tRNA            complement(1976..2057)
                     /locus_tag="PLW15_08500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2021..2023),aa:Leu,seq:tag)
     gene            2097..2675
                     /locus_tag="PLW15_08505"
     CDS             2097..2675
                     /locus_tag="PLW15_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOT34472.1"
                     /translation="MTPDDAAAELGVPVGASRQQIERAYRRLARESHPDRLSGEAMRE
                     QALAQARFVRATEARNVLLATLLTTDAAASATRLPPKHNWLLLGTWVALVLLAIAVSV
                     VGSPLPLTIWEPTLRFSLMVVSAVGFALTGARVWLVLLGVAIAVTALTTIVFTTFGAL
                     LGMLLLAAPLYGLFVAGRARAGFSRDRRPSPR"
     gene            2685..2900
                     /locus_tag="PLW15_08510"
     CDS             2685..2900
                     /locus_tag="PLW15_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34473.1"
                     /translation="MASVRAAYESTVQRHYTIAAILIAAAIALIAGVVVGMLIGGAGL
                     GIPGSSAFSSITGGSVGGGTEPRQGGR"
     gene            2991..3854
                     /locus_tag="PLW15_08515"
     CDS             2991..3854
                     /locus_tag="PLW15_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT34474.1"
                     /translation="MSIYAVTGSASGMGKATADRLREHGHTVIGVDLRDADIIADLSK
                     PEGRAFAASEVLHQSGGKLRGAVLAAGIGPGPGRTRALAEINYLGAVELLQAWRPALA
                     ATASAKVVVFSSNSTTTTPLIPGSAIRALLDGNAAKAERIMGRFGKNAPSLMYGTSKI
                     ALSRWLRRAAVTPEWAGAGIRLNAIAPGAILTPLLQAQLDNPEQRGAIEAFPIPTGTY
                     GTADHIADWVIFMLSPAADFMAGSVVFVDGGTDAYFRSDDWPRGVPLRGLQRYLRLTK
                     QFGAQKAASAK"
     gene            3907..5130
                     /locus_tag="PLW15_08520"
     CDS             3907..5130
                     /locus_tag="PLW15_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT34475.1"
                     /translation="MSTALASIADRVRERVRREGVDLSPELADRYVREEVRSYSERAL
                     GGSLPMLDDETDATRQVIATLTGFGALQPLLDDPDIEELWINAPDAVFVARGGVSEAT
                     GIRLTDGQVRDLVERMLQSSGRRVDLSSPFVDASLPDGSRLHVAIPDVTRRHWAVNVR
                     KFSTRIRDLSALVGLGSLTQQAAEFLRMCVLANQNILVSGATQSGKTTMLSALLASGR
                     PTERIITVEETFELVVLGRDSVALQCRQPSLEGTGEITLRRLVKEALRMRPDRLVVGE
                     VREAESLDLLIALNSGLPGMSSIHANNARDALTKLCTLPLLAGRNIDSAFVVPTVASS
                     IDVVVHCELVRGGRRRVVEILAPTGQVTSGVIEASPIFTLEHGSLEHTGGYPSRTTKF
                     AAAGLDPALVLGSRR"
     gene            5127..>5392
                     /locus_tag="PLW15_08525"
     CDS             5127..>5392
                     /locus_tag="PLW15_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein F"
                     /protein_id="HOT34476.1"
                     /translation="MTALVGLMLGGGLMLVLSPGLWPRGEGRGIRPPQLASVRERLVQ
                     AGLHRVSPAVVVVVSALLGVVGFAVVLALTGVVVVATAAGILAAT"
BASE COUNT          836 a         1781 c         1852 g          923 t
ORIGIN      
        1 cgacacgaag aacggtacgg tggttacgga tgtggattac tgctacgact gggctgatcg
       61 tctgacgtcc acgaacgcaa ccacgacggg tggtaacccg gtgttgaaca cggacctgtc
      121 caccgtcggt gcccccgcga ctttggcgta tgacacgcac ggcaacacga cgaagctcgc
      181 cgatcaaacc ctcgcctatg acgtcgctga ccggcacatg tcgacggtgt tggctgacgg
      241 tacgacgatc acctatgccc gggatgccac gggccgggtc gtgtctcgca tcacagacgc
      301 cccaggcacc acgaatgatt cgacgatccg gtactcgttc gccggtggct ccttgtttgc
      361 ggtgttggac gggtcgaacc aggtgctcca acgcgagcta tccctacccg gcggggtctc
      421 actgtcgatc ccggctgcgg gtgggcaggt gtgggcgtac ccgaacctgc acggcgatgt
      481 gatcctgacc acggatgcca cgggcatccg ccaaggcacc cgcaccagtt atgacccgtt
      541 cgggcagccc ctcgccgcta atggtgacat cggttcgacg gtcgcggatg acagcatccc
      601 ggacaccagc ccgggggatg ccgactacgg gtatgtcggg cagcacgaca aactctacga
      661 acaccaaggg tcgctcgcga caatcgagat gggggtgcgg cagtacgtcc ccgcgttggg
      721 gcggttcctg tcctgcgatc ccgtcgaagg gggagtcacg aatgcttatg actatccgtc
      781 tgactcgatc aactctttcg atctctcggg ccgagctcaa atggggcaca caatgctcga
      841 ctcgagcggg tctcggggtt ctccgccgag acccgaaccg gagggtcacg gtcgttcggg
      901 ccccacaccc ctgcaacggg cttcggacgc aaatcaatcg actacacaag ccgccatcca
      961 gctcgcactg gccaccggag gaacatgctc gtacgtccca aatcagccga tggtgttctg
     1021 caccggaatc acagggatac agctcggtgc ttacaccaca attgggaacg cggtgctcac
     1081 gaacggaacc catggtccct ctggcgacgt gttgacccac gagcagtatc acatgtacca
     1141 atgggcggag atgggtgcct accggttcac ggcggcgtgg atcggtgggg agttatattc
     1201 gcagcgcgcc caagaacaag gctggctccc ggacaactgc cgctctgagc gagggtgcct
     1261 aaatccgatc gagattgcgg cggggccgta cattgggaac tactgggcgc gccccgacgc
     1321 gtggccgcag ggagcgaggt aatcaatggt caggagagtt gcaaccgcaa tggtggcgtt
     1381 gctggcaatc gccttgtcgg gctgcacaag tgctccaagc ctggtgtcgg cgaaactcga
     1441 tggcgattta gtcgtatttg ccgtatgtga acagatcgct tatgaccaac tgaccgtgct
     1501 atccgatgag aaggggtcga ttccatccag tttcggggag acttgggttg caaagggttt
     1561 gggagcaaca ggcggtttcg tggagattcg ctacggagtt ccaccgcgcg ggttagagac
     1621 aagggtcggc ccggacgcgc tccctaccag cggccattac gtccactttg aactgatcgg
     1681 ccacgacgat gagggtgcga ttgcgggcga tctcttcgcg cagttcgacg gagacaagct
     1741 tgtgccagag aggtggctta actcttccgg cgagttacgc gagagtccgt gtcgctaggc
     1801 ctttgttgac cgtgaggtag attccgtcgg cggaaggcgg gacttgaacc cgccccaccg
     1861 gcacggccgt cacacggctt cgccgtgcgc cggaaccggc gcggcggggg ccccggtgcg
     1921 tttcgcgccc gccgtccgga ctgatggccc gacgccgatg gcgccagtcc atcagtgcgg
     1981 aaggcgggac ttgaacccgc acgctcgaaa gcacaggaac ctaaatcctg cgtgtctgcc
     2041 aatttcacca ctcccgctgg caggtcgatt gtagccgccc ggcgtacgct ggcgtgatga
     2101 cccccgacga cgctgccgcc gaactcggcg tgccggtcgg ggcgtctcgg cagcagatcg
     2161 agcgggcgta ccggcgactc gcgcgcgagt cgcaccccga tcggctgtcc ggggaggcga
     2221 tgcgcgagca ggcgctggct caggcgcgat tcgtgcgggc gaccgaggcg cggaacgtgc
     2281 tgcttgccac cttgctgacg acggatgccg cggcatccgc aacccgtctg cccccgaaac
     2341 acaactggct gttgctcggc acctgggtcg cgctcgtgct gctagcgata gccgtgtcgg
     2401 tcgtgggatc cccgctaccg ctgaccatct gggagcccac cctgcggttc tccctgatgg
     2461 tcgtctcggc ggtcgggttc gccctcaccg gcgcacgcgt gtggctcgtg ctgctcggtg
     2521 tggcgatcgc cgtgactgcc ctcacgacga tcgtcttcac caccttcggg gccctgctcg
     2581 ggatgctgct gctcgccgct ccgctctacg ggctgttcgt ggcgggccgc gcccgcgccg
     2641 gattctcccg cgacaggcga ccctcgccgc gctagcctgt aggcatggca tcggttcgcg
     2701 cggcatacga gtccacggtc cagcggcact acacgatcgc ggcgatcctc atcgcggcgg
     2761 cgatagccct gatcgcgggt gtcgtcgtcg gcatgctgat cggtggcgct ggcctgggca
     2821 ttcccggctc gagcgcgttc tcgagcatca ccggcggctc cgtcggtggg ggcaccgagc
     2881 cgcgtcaggg cggccgctaa ccccaagccg ctaaccctgg ctgcgaacgc cggccgccgg
     2941 gggacttccg gccctgtggc gtgcgcggca gcatccctag cctcggtttc atgagcatct
     3001 acgccgtcac cggatccgcc tcgggaatgg gcaaagccac cgccgatcgg cttcgcgagc
     3061 acggccacac cgtgatcggt gtcgacctgc gtgacgccga catcatcgcc gacctctcga
     3121 agccggaggg gcgcgcgttt gccgcatcgg aggtgctgca ccagtcgggc ggcaagctac
     3181 gcggtgccgt gctcgcggcc gggatcgggc ctggcccggg tcgcacgcga gcgttggccg
     3241 agatcaacta cctcggtgca gttgagctgc tgcaggcgtg gcgtcccgcg ctcgccgcga
     3301 cggcctcggc gaaggttgtc gtgttctcgt cgaactcgac gacgaccacg ccgctgatcc
     3361 ccggctccgc gatccgcgcc ctgctcgacg gcaacgcggc gaaggccgag cgcatcatgg
     3421 gccgcttcgg caagaacgcg ccgtcgctca tgtacggaac gtccaagatc gcgctgagcc
     3481 gctggctgcg ccgcgcggcg gtcactccgg agtgggctgg ggcgggcatc cgtctcaatg
     3541 cgatcgcgcc gggggcgatt ctcaccccgc tgctgcaggc ccagctcgac aacccggaac
     3601 aacgcggcgc gatcgaggcg ttcccgatcc cgaccggcac ctacggcacc gccgaccaca
     3661 tcgccgactg ggtgatcttc atgctgagcc ccgcggccga cttcatggcc ggctcggtcg
     3721 tcttcgttga cgggggtacg gatgcttact tccgcagcga cgactggccc cgcggcgttc
     3781 cgctgcgcgg gttgcagcgc tacctgcgtt tgaccaagca gttcggtgcg cagaaggctg
     3841 cgtcggcgaa gtagcggtgt ggacaactcg gccccctgac cgggcggtcg ccccgagaat
     3901 ggcggcatgt ccaccgccct cgcctccatc gctgatcgcg tgcgcgaacg ggtgcgccgg
     3961 gagggtgtcg acctgtcacc cgagctcgcc gaccgctacg tgcgcgagga ggtgcgcagc
     4021 tacagcgagc gcgctctcgg cgggtcgctt ccgatgctcg acgacgagac ggatgccacc
     4081 cgccaggtca tcgccacgct gaccggtttc ggcgcgctcc aacctctgct cgacgatccc
     4141 gacatcgagg aactctggat caacgcaccc gacgccgtgt tcgttgcgcg cggcggggtg
     4201 agtgaggcca cgggcatccg gctgacagac gggcaggtgc gcgatctggt cgagcggatg
     4261 cttcagagct cgggccgtcg cgtcgacctg agttccccgt tcgtcgacgc ctcgctgccg
     4321 gacggttcgc gcctgcacgt cgcgatcccg gacgtgactc gccgccactg ggcggtcaac
     4381 gtgcgcaagt tcagcacgcg catccgcgac ctgtcggcgc tcgtgggcct cggcagcctc
     4441 acgcagcagg cggccgagtt tctgcggatg tgcgtgctcg ccaaccagaa catcctcgtg
     4501 tcgggcgcga cgcagagcgg caagaccacg atgctctcgg cgctgctggc gtcgggtcgg
     4561 ccgaccgagc gcatcatcac cgtggaggag acgttcgaac tggtcgtgct cggccgcgac
     4621 tcggtcgcgc tgcagtgccg gcagcccagc ctcgagggca ccggcgagat caccttgcgc
     4681 cgcctcgtga aagaggccct gcggatgcgc cccgaccgac tcgtggtggg tgaggtgcgc
     4741 gaggccgagt ccctcgacct gctcatcgcg ctcaacagtg ggctgcccgg tatgtcgagc
     4801 atccacgcca acaatgcacg ggatgccctc acgaagctct gcacgctgcc cctgctggcg
     4861 gggcgcaaca tcgactcggc gttcgtggtg ccgactgtcg cgtcatccat cgacgtggtc
     4921 gtgcactgcg agctggtgcg tggcgggcgg cggcgggtgg tggagatcct ggcgccgacc
     4981 gggcaggtca cgtcgggtgt gatcgaggcg agtccgatct tcacgctcga gcacgggtcg
     5041 ctcgagcaca ccgggggata cccgtcgcgc acgacgaagt tcgcggcggc ggggctcgac
     5101 ccggcgctgg tgctcgggag taggcggtga ccgcgctggt ggggctgatg ctcggcggcg
     5161 ggctgatgct ggtgctatca cccgggctct ggccgcgggg ggagggacgc ggcatccggc
     5221 cgccgcaact cgccagcgtg cgggagcggc tggtgcaggc ggggctgcac cgggtcagtc
     5281 ccgccgtggt cgtggtggtg tcggccctgc tcggggtggt cgggttcgcg gtggtgctcg
     5341 cgctcaccgg ggtggtcgtc gtggcgacgg ccgcgggcat cctcgcggcg ac
//
LOCUS       DAOSOU010000151         5360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15400_length_5360_cov_6.337983, whole genome shotgun sequence.
ACCESSION   DAOSOU010000151 DAOSOU010000000
VERSION     DAOSOU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15400_length_5360_cov_6.337983"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PLW15_08530"
     CDS             <1..1177
                     /locus_tag="PLW15_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT34477.1"
                     /translation="VGGLYVLGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLQDDL
                     MRLFNSGAAEALMGRSAMPDDMAIESKVVSRAIRSAQSQVEARNAEIRKNVLKYDDVL
                     NRQREAIYSDRRHILEGDDLQDRVSKFLDDVITEVIEVHTGEGHSDDWDFDALWTELK
                     TLYPISITVDEVLSEAGTRGRVTTAFLTKEILSDARLAYRRREQQLGEPAMRELERRV
                     VLSVIDRRWRDHLYEMDYLKDGIGLRAMAQRDPLVEYQREGFALYQSMMAAIREEAVG
                     FLFNLEVEVTGGTAGPTVQARGLQAPAAPVNQLTYTAPSEDASGDLEVRNQRGQVERA
                     ATARAQQAAAVQQRAVAPQQPQPQQPAARGAFGQRTDDGTPPPQNRAERRAQERRGN"
     gene            1280..1846
                     /locus_tag="PLW15_08535"
     CDS             1280..1846
                     /locus_tag="PLW15_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34478.1"
                     /translation="MAQHPITHTTLISVLSAAVLAVALTACSSPSVPADQPTNGPTAG
                     AEQPSDVPAGTGDFVPDDAWFTAVDDARTSLSDFLGFWDAQDCTMDKVVAGDFNCNIQ
                     ISGIVQGVNDLAPLLADTVNIAPDSTASIAGLEGSAPTAAQAATDTAAYSDAVCDFAP
                     DDACTPLGDAVVASATALSVEIAGWDAP"
     gene            complement(1917..2342)
                     /locus_tag="PLW15_08540"
     CDS             complement(1917..2342)
                     /locus_tag="PLW15_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HOT34479.1"
                     /translation="MSSALPSKVIRARFEPDEFFGLQPTSTSSLPNPAPLVENLTRCV
                     IEILAGARDLEQISRWVTDDVYRHLLKRVVLSARARQAKGQPIARPTFGIGSTTICEP
                     ADGVVEAVVIVRGRARTRAVAIRLEGMDNRWRASAINVL"
     gene            2495..3082
                     /locus_tag="PLW15_08545"
     CDS             2495..3082
                     /locus_tag="PLW15_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34480.1"
                     /translation="MRWDHLFDDLEGQLERELTAEEQDLGIEEERLRLGRLSVRDRIA
                     ALHEAGHPIGLTLTTGERLVADVDTLGRDWFSATVAEGAVRSARCLVPLASIASLVLA
                     PGQVEPSLVPVRDARAEASLSMRLGLPFVLRDLCRRRWALDLVVGATTLHGTIDRVGR
                     DHLDLAMHEPGSARRNTAVREVRVVPLQQIVLVSY"
     gene            complement(3104..3376)
                     /locus_tag="PLW15_08550"
     CDS             complement(3104..3376)
                     /locus_tag="PLW15_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT34481.1"
                     /translation="MNDATSAGLGRFLTLADAADLLNVTPTQVRELVLSGELPAIRVG
                     AQGQWRVERTVLEGYIDALYEQARRLSLWNQAEFDQLPEYTFGETQ"
     gene            3487..4107
                     /locus_tag="PLW15_08555"
     CDS             3487..4107
                     /locus_tag="PLW15_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34482.1"
                     /translation="MSRSPARAFLLDPRLLIGVLLVAGSVAGVLAIVSAADDSITVLA
                     APAALAPGDRVGESDLEVRSVRLDSSADRYLAPGDVPAEGVVVTRVIGAGELVPVDAV
                     GEVDGVRLAPLVLSVEGALAASVEPGASVDVWAAQAVDGGFGEPGVVVTGALVVRLVE
                     TRTIVTGGGVTGIEVLVPRTRIAAVLAAVANDAALSIVPASIPLGG"
     gene            4112..5311
                     /locus_tag="PLW15_08560"
     CDS             4112..5311
                     /locus_tag="PLW15_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HOT34483.1"
                     /translation="MSLVLGLPADHEARLRSDATRYGHEVLASSGSAQELEVVVRALR
                     PGAAVVSASPQYLTPRVLAACDDLGVRVVALVADEMGRRHAASLGLYETTDATADWPT
                     IELMLGGSRGPAPVTAPAVTRGQVIAVWGPSGAPGRTTLAIGIASELAALGHRVALAD
                     VDTHGAAVAPTLGLLDEAPGFAAACRLASSDTLTPEELDRVSDVYQGASSFRVLTGIG
                     APRRWPELSPERVTGVIQRCREWVDFTVIDMASSLENDEEISSDLFAPRRNGATVAAL
                     READRVVAVGSADPVGMSRFLRAHVELLEVSGAGRVDVVMNRVRASAIGVGPAAQVRQ
                     TLARFGGIDDPVLVPHDQNAVDGAVLSGRTLLDVSPRSGARRAIADLVALRLAPVAEP
                     ARGRRRR"
BASE COUNT          762 a         1861 c         1920 g          817 t
ORIGIN      
        1 ggtcggtggt ctgtacgtgc tcggcaccga gcgccacgag tcgcgccgca tcgacaacca
       61 gttgcgcggt cgctccggcc gtcagggtga cccgggcgag agccgcttct acctgtcgct
      121 tcaggatgac ctcatgcgcc tgttcaactc cggtgccgcc gaggcgctca tgggccgcag
      181 cgcgatgccg gacgacatgg caatcgagtc caaggtcgtg agccgcgcca tccgcagcgc
      241 acagagccag gtcgaggcgc gcaacgcgga gatccgcaag aacgtgctca agtacgacga
      301 tgtgctcaac cgccagcgcg aggcgatcta cagcgaccgc cgtcacatcc tcgagggcga
      361 cgacctgcag gatcgcgtct cgaagttcct cgacgacgtg atcaccgagg tcatcgaggt
      421 gcacaccggt gagggtcact ccgacgactg ggacttcgat gccctgtgga ccgagctgaa
      481 gaccctttac ccgatctcga tcacggtcga cgaggtgctc agcgaggctg gcacccgcgg
      541 gcgcgtcacc acggcgttcc tcaccaagga gatcttgtcg gatgcccggc tcgcctaccg
      601 ccgccgcgag cagcagctcg gcgagcccgc catgcgcgag ctcgagcgtc gcgtggtgct
      661 cagcgtcatc gaccgccgct ggcgcgacca cctctacgag atggactacc tcaaggacgg
      721 catcggactg cgcgcgatgg cgcagcgcga cccgctggtc gagtaccagc gtgagggctt
      781 cgcgctgtac cagtcgatga tggcggcgat ccgcgaggag gccgtcggct tcctgttcaa
      841 cctcgaggtc gaggtgaccg gcggaacggc cggacccacc gtgcaggccc gcggactcca
      901 ggctcctgcc gcgcccgtca accagctcac ctacacggct ccgagcgagg acgcgtcggg
      961 cgatctggag gtgcgcaacc agcgcggcca ggtcgagcgg gcggccaccg ctcgcgccca
     1021 gcaggcggcg gcagtgcagc agcgcgcggt tgctccccag cagccgcaac cgcagcagcc
     1081 cgccgcgcgt ggagcgttcg gtcagcgcac cgacgacggc acccctccgc cgcagaaccg
     1141 cgccgagcgt cgtgcgcagg agcgccgcgg caactagcag ctcctcgctg gcccgctcgg
     1201 ggcatcagtg gaacgactga tgcccgaatg gcgactcaac cgatgcggat cgccgctcag
     1261 gttgcctaga gtattcgtca tggctcagca ccccatcaca cacacgaccc tgatctccgt
     1321 cctgtctgcc gcggttctcg cggtggccct caccgcctgt tcgtcaccgt cggttcccgc
     1381 cgaccaaccg accaatgggc ccacagccgg cgccgagcag ccgagcgacg tgcccgctgg
     1441 tacgggcgac ttcgtgcccg acgacgcctg gttcacggcc gtcgacgatg cgcgcacctc
     1501 gctgtccgac ttcctcggat tctgggatgc gcaggactgc accatggaca aggtggtggc
     1561 aggcgacttc aactgcaata tccagatctc gggcatcgtg cagggtgtca acgacctggc
     1621 tccgctgctg gcggacaccg tcaacatcgc tcccgattcg accgcgtcga tcgccggcct
     1681 cgaggggtcg gccccgaccg cggcgcaggc agcgaccgac acagccgcct actccgatgc
     1741 cgtctgcgac ttcgcaccgg atgacgcgtg cacccccctc ggcgacgccg tggtggcctc
     1801 ggccaccgcg ctgagcgtgg agatcgccgg ctgggacgcg ccgtagcctg cgcgggcgcg
     1861 ggcgctggcc tgcgccggcc ctcgcctgcg tgagccctac cagagcgagt ccggcgtcag
     1921 agcacgttga tcgcgctcgc ccgccagcgg ttgtccatgc cctcgagcct gatcgcgacc
     1981 gcccgggtgc gcgcgcggcc gcgcacgatc accacggcct cgacgacgcc atccgctggc
     2041 tcgcagatgg tcgtggaacc gatgccgaat gtcgggcgtg cgatcggctg gcccttcgcc
     2101 tgccgggcgc gggcggagag cacgacgcgc ttgagcaggt gacggtagac gtcgtcggtg
     2161 acccagcgcg agatctgttc gagatcgcgt gctccggcga gaatctcgat cacgcagcgc
     2221 gtgaggttct cgaccagggg cgcaggattg ggcagtgagc tggtcgaggt cggctgcagg
     2281 ccgaagaact cgtcaggttc gaaccgcgcg cggatgacct tggacggaag cgctgaactc
     2341 atcgacaccc acctcacaga attgccagaa attcctgtca accctgcggc gggtagagac
     2401 cacctaagca ggttggggcc cctctgtcca tgctcgcgat caagctgtgg ataacccgtt
     2461 tcccagccgg gatgtgccgg tctagggtca ccccatgcgc tgggaccacc tcttcgatga
     2521 ccttgagggg cagctcgagc gcgagctcac cgcggaggaa caggatctcg gcatcgagga
     2581 ggagcggctg aggttggggc ggctgtcggt gcgcgaccgc atcgcagcgc tgcacgaggc
     2641 ggggcatccg atcggtctga cgctcacaac cggtgagcga ctcgtcgccg acgtcgacac
     2701 cctcggccgc gactggttct cggcgacggt cgcagagggt gcggttcgca gcgcgcggtg
     2761 cctcgtgccg ctcgcgtcca tcgcctcgct cgtgcttgcg ccgggtcagg tggagccgag
     2821 tctggttccg gtgcgggatg cccgtgccga ggcatccctg tcgatgcggt tggggctgcc
     2881 gttcgtgctc cgcgacctct gtcgtcggcg gtgggctctc gatctcgtgg tcggcgcgac
     2941 caccctgcac ggaacgatcg acagggtcgg tcgcgaccac ctcgacctgg cgatgcacga
     3001 gcccggcagc gcccgacgca acacggcggt gcgcgaggtg cgggtggtgc cgctgcagca
     3061 gatcgtgctg gtgagctatt gacgccggtg tgaggcgggg ccgctactgc gtctcgccga
     3121 aggtgtactc gggcagctgg tcgaactcgg cctggttcca caggctgagc cggcgggcct
     3181 gctcgtagag ggcatcgatg tagccctcga gcaccgtgcg ctcgacgcgc cactggccct
     3241 gggcccccac gcggatcgcg ggcagctcgc cggagagcac gagctcacgc acctgggtgg
     3301 gggtgacgtt caggagatcg gccgcgtcag ccagcgtcag aaaccgaccg aggcccgcgg
     3361 aggtggcatc gttcatgcct cgattatgcg cgcgcacgcc gcgcgcgaac gggtgtgtgg
     3421 ataactcgcg ccgccgctca cggccgatcg cgagcatggg ctgtccgacg aaggaagagg
     3481 tcagccatgt cccgcagccc cgcccgagcg ttcctgctcg acccccggct actgatcggc
     3541 gtgctgctgg tggcggggtc agtcgctggg gtgctcgcca tcgtgtcggc cgcagacgac
     3601 agcatcaccg tgctcgcggc gccggcggct ctggccccgg gtgatcgcgt gggcgagtcc
     3661 gacctcgaag tgcgcagcgt gcgactcgac tcgtcggccg accggtacct cgcccccggc
     3721 gatgtgccgg cggagggggt cgtggtgact cgggtgatcg gtgcgggcga gctggtcccc
     3781 gtcgacgcgg tgggtgaggt cgacggcgtt cggctcgcgc cgctcgtgct cagcgtggag
     3841 ggggcgctcg cggcatccgt ggagcccgga gcgtcggtgg acgtctgggc ggcgcaggcc
     3901 gtcgacggcg gcttcggaga acccggggtg gtcgtcaccg gcgcgctcgt cgtgcggctg
     3961 gtggagacac gcacgatcgt caccggcggg ggagtgaccg gcatcgaggt gctcgttccg
     4021 cgcacccgga tcgccgcggt tctcgctgcc gtcgccaacg atgcggcgct gtcgatcgtg
     4081 cccgcctcga tcccgctcgg cggctgaggt ggtgtcgctc gtactcgggc ttcccgccga
     4141 ccacgaggcg aggctgcgct ccgacgcgac ccgctacggt cacgaggtgc tcgcaagctc
     4201 cggcagcgcg caggaactcg aggtggtggt gcgggctctt cgccccggcg cggcggtggt
     4261 gtccgcgagc ccgcagtacc tgacgccccg ggtgctcgcc gcgtgcgacg acctgggggt
     4321 gcgggtggtc gcactcgtcg ccgacgagat gggtcggcgg cacgcggcat ccctgggcct
     4381 gtatgagacg acggatgcca cggccgactg gccgacgatc gaactcatgc tcggcggctc
     4441 gcgcggcccg gcccccgtca ccgcgccggc tgtcacacgg ggtcaggtca tcgccgtctg
     4501 gggaccgtca ggcgcacccg gccgcacgac tctcgccatc ggcatcgcga gcgaactggc
     4561 ggcgctcggg caccgcgtcg cgctcgccga tgtcgacacg cacggtgccg cggtcgcgcc
     4621 gaccctgggg ctgctggatg aggctcccgg cttcgcggcc gcctgtcgcc tcgccagcag
     4681 cgacaccctc acccccgagg aactggaccg cgtgagcgac gtctaccagg gcgcatcgag
     4741 cttcagggtg ctgaccggaa tcggtgcgcc ccggcgttgg cccgaactct cgccggagcg
     4801 ggtgacgggc gtgatccagc gctgccgcga gtgggtcgac ttcacggtga tcgacatggc
     4861 gtcgagcctg gagaacgacg aggagatctc gagcgacctc ttcgccccgc ggcgcaacgg
     4921 cgcgaccgtc gccgcactgc gcgaggcgga ccgggtggtc gccgtgggtt cggccgaccc
     4981 ggtggggatg tcccgctttc tgcgcgctca cgtcgagctg ctggaggtca gcggcgccgg
     5041 gcgcgtcgac gtcgtcatga accgggtgcg ggcatccgcg atcggcgtcg ggccagcggc
     5101 gcaggtgcgt cagacgctcg cgcggttcgg tggcatcgac gacccggtgc tcgtgccgca
     5161 cgaccagaac gcggtcgacg gggccgtgct cagcggccgg acgctgctcg acgtgtcgcc
     5221 gcgctcgggt gcgcgtcggg cgatcgctga cctggttgcg ctgcggctcg cgcccgtcgc
     5281 tgagcccgcg cgggggcgac ggcgccgcta gggcgctagc cttgaacgcg tgtcgaccct
     5341 cagtgatctc gttcaggccc
//
LOCUS       DAOSOU010000152         5341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15483_length_5341_cov_7.316686, whole genome shotgun sequence.
ACCESSION   DAOSOU010000152 DAOSOU010000000
VERSION     DAOSOU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15483_length_5341_cov_7.316686"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLW15_08565"
     CDS             <1..1082
                     /locus_tag="PLW15_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOT34484.1"
                     /translation="HPMSVRVALAGVGNCANSLVQGVTFYADAAADATIPGLMHNRLG
                     PYAVGDVEFVAAFDVDALKVGEDLADAIWASQNSTFRFAEVAPTGVTVQRGPTLDGLG
                     EYYREVVTESDAPAVDIAQALRDSGADVLVAYLPVGSDLAAKHYAQAALDAGVAFVNA
                     LPVFIASDPEWAAKFTAAGIPIVGDDVKSQLGATITHRVLARLFEERGLVLDRTYQLN
                     VGGNMDFKNMLERSRLESKKVSKTQAVTSNVEAELNARDVHIGPSDHIPWLDDRKLAF
                     VRLEGHGFGNAPTSLEYKLEVWDSPNSAGVVIDAIRAAKVALDAGLGGPVEAASAYLM
                     KSPPVQLPDPVARARFDEWISANSR"
     gene            complement(1222..2640)
                     /locus_tag="PLW15_08570"
     CDS             complement(1222..2640)
                     /locus_tag="PLW15_08570"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOT34485.1"
                     /translation="MESVARAVERLGELADSAAVSRLAAAFSAAGHELALVGGSVRDA
                     FLNRDLTDLDFTTSARPDDILALVTPISDVQWDVGRDFGTIAARINGLTVEITTYRAD
                     AYDGETRKPIVQFGDTLEDDLVRRDFTVNAMALRLPERQLVDPWGGLDDLLARRISTP
                     GAPEVSFGDDPLRMLRAARFTSQLGFTVADDTVRAMAELAPRLEIVSVERISDELSKL
                     LRTDDPVPGIRLLVDTGIAQLVLPEIPALRLEVDEHAHHKDVYEHSLTVLRQAIDLEK
                     SRGHSVDLVLRLAALLHDIGKPSTRRIEGGGVVTFHHHDVVGAKLAAKRLKALRFDND
                     TVASVARLIELHLRFFGYTDGAWTDSAVRRYVRDAGDQLERLHILTRADVTTRNRRKA
                     DRLGFAYDDLEERIAAIAEAEGIAAVRPDLDGEQIMAILGLAPGREVGEAYRFLLDLR
                     LDEGPLGPEEAEVRLRAWWSAR"
     gene            2710..4356
                     /locus_tag="PLW15_08575"
     CDS             2710..4356
                     /locus_tag="PLW15_08575"
                     /inference="COORDINATES: protein motif:HMM:NF039636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HOT34486.1"
                     /translation="MLAERFTPLRAALALLLGLAGAVTGVAIAAPAATSVGPDGTVAL
                     TVVVPLAVPESDAGFIPADALDQYTRPTGLLTRELDAIIDRPVVIAIDPRIIASIRIL
                     GSSAPASAIEWLSRLESASNETFALTYADSDITLGLQAGSPRVLQPESFDFAIDPSLF
                     AEAAAQTPAPTPSPDATAPGLPTSESLLAWPYTLESIAWPRDGTVVTADLSAITASGY
                     TTTILSSANVSRDEGSALVRVDGGTAAISDDAVSGALRTAVASVSPEDWVSAMATVGS
                     ALDAAPATVLATLDRTVPITGTHLAETLTTLQADPRVRLTGLSQLLAGSPDEATLVDS
                     PQPAASVERMTMLLDAESAEIRFATVAESPSAITSPRRLHLLALLSSAWLRNPAGWLT
                     ATDAFLTDSDDLRASVQVVAYSSFNLLADSASLPISVSNSLDQAVTVYITVRPETALL
                     AVTDSRVELVVEPDSQAKGQIPVQAVSNGTVQVLITLASGAGVPIGAPATAEINVQAG
                     WETPIVLVVGIIVVLVFGGGIVRNILRRRKPARPVEPTDD"
     gene            4349..>5341
                     /locus_tag="PLW15_08580"
     CDS             4349..>5341
                     /locus_tag="PLW15_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOT34487.1"
                     /translation="MTDRPSDEVDGASAIGRASAALASGTIVSRILGFVNAAVLAAAI
                     GYIGGAADAFTLATQIPNSIYALIAGGLLSAVLVPQIVRAGIQADGGAAFINKLVTLG
                     TVVFVIVAAAATIAAPWLVSLYAQSGTKGFGPDELALATAFAYWCLPQVLFYALYSLL
                     GQVLNARRVFGPFTWAPALNNVIAITGVSIFIALFGAFPANQDASVWTPTMIAVLGGT
                     ATLGIAAQAFILLFFWRRAGLTYRPDFRWRGVGLGSTGRAAAWVFGMILITQLAAIVE
                     TRVATGASGDASIGALKYAWLIFMLPHSIVTVSIATAYFTRMSTHARGGDLASIR"
BASE COUNT          802 a         1881 c         1785 g          873 t
ORIGIN      
        1 accatcccat gtctgtgcgc gtagcccttg cgggtgtcgg caactgtgcc aactcgcttg
       61 ttcagggcgt gaccttctac gcggatgccg cggccgacgc cactatcccc ggtctcatgc
      121 acaatcggct cgggccgtac gccgtcggtg acgtcgagtt cgtcgccgcg ttcgacgtgg
      181 acgccctgaa ggtcggcgag gacctggccg acgccatctg ggcgagccag aactcgacct
      241 tccgctttgc cgaggtcgca cccaccggtg tgaccgtgca gcgcggaccc accctcgacg
      301 ggctgggcga gtactaccgc gaggtcgtca ccgagtcgga tgcccccgcc gtcgacatcg
      361 cgcaggccct ccgcgacagc ggcgccgatg tgctcgtggc ctaccttccc gtcggctcgg
      421 acctcgcggc gaagcactac gcacaggccg ccctcgacgc cggggtcgcc ttcgtcaatg
      481 ctctaccggt gttcatcgcg agcgacccgg agtgggcggc gaagttcacc gcggcgggca
      541 tcccgatcgt cggcgacgac gtgaagagcc agctcggcgc aaccatcacc caccgggtgc
      601 tcgcccgact gttcgaggag cggggactcg tgctcgaccg cacctaccag ctcaacgtcg
      661 gcggcaacat ggacttcaag aacatgctgg agcgctcccg actcgagtcg aagaaggtgt
      721 cgaagacgca ggcggtcacc agcaatgtgg aggccgagct gaacgcccgc gacgtgcaca
      781 tcgggccgag cgaccacatc ccgtggctcg acgaccgcaa gctcgcgttc gtgcgcctcg
      841 aggggcacgg cttcggcaac gcgcccacga gcctcgagta caagctcgag gtgtgggact
      901 cgccgaactc cgccggcgtc gtgatcgacg cgatccgggc ggcgaaggtg gcgctcgacg
      961 ccggcctcgg gggaccggtg gaggcggcat ccgcctacct gatgaagtca ccgccggtgc
     1021 aactgcccga cccggtcgcc cgcgcccgct tcgacgagtg gatcagcgcg aactcccgtt
     1081 gatctgacgg ctctcgcccg ttcgcggatc aggagttcgc gcagacacac cgtgcctgtg
     1141 ccgcgttcta gcggcgagcc gcgcagatct cctgatccgc gaacagttca ggggagacgg
     1201 gagtccgagc atctcgtgct ctcagcgcgc agaccaccac gcccggaggc gcacttcggc
     1261 ctcctcgggc ccgagtggac cctcgtcgag gcgcaggtcg agcaggaagc ggtacgcctc
     1321 tcccacctcg cgcccgggcg caagcccgag gatcgccatg atctgctcac cgtcgagatc
     1381 gggccgcacg gccgcgatgc cttcggcctc ggcgatcgct gcgatgcgct cctcgaggtc
     1441 gtcgtaggcg aacccgagcc ggtcggcctt gcgccggttg cgggtcgtca cgtcggcccg
     1501 ggtcaggatg tgcaacctct cgagctggtc gcccgcgtcg cgcacgtagc gacgcaccgc
     1561 cgagtcggtc cacgcgccat ccgtgtagcc gaagaaacgc aggtgcagct cgatcagtcg
     1621 ggcgacggat gccacggtgt cgttgtcgaa gcgcagcgcc ttgagtcgct tggccgcgag
     1681 cttggcgccg acgacgtcgt ggtggtggaa ggtgaccaca ccacccccct cgattcgtcg
     1741 ggtggagggc ttgccgatgt cgtgcagcag ggccgcgagg cgcagcacga ggtcgaccga
     1801 gtgtccgcgt gacttctcca ggtcgatcgc ctggcgcagc acggtgaggc tgtgctcgta
     1861 gacgtccttg tggtgagcgt gctcatcgac ctcgagtcgc aacgccggga tctcggggag
     1921 caccaactgc gcaatgcccg tgtcgacgag cagacggatg cccggcaccg ggtcatccgt
     1981 gcgcagcagc ttcgacagct catcgctgat gcgctcaacg ctcacgatct ccaaccgcgg
     2041 cgcgagctca gccatcgcgc ggacggtgtc gtcggccacg gtgaacccca gctggctcgt
     2101 gaaccgggcg gctcgcagca tccgcaacgg gtcatcgccg aaggagacct ccggtgctcc
     2161 gggggtcgag atgcggcggg cgagcaggtc atcgagacca ccccaggggt cgacgagctg
     2221 ccgttcgggc agccggaggg ccatcgcgtt gacggtgaag tcgcgccgca cgaggtcgtc
     2281 ctcgagtgtg tcgccgaact gcacgatcgg cttgcgggtc tcgccgtcgt aggcgtcggc
     2341 ccggtaggtc gtgatctcta ccgtcagccc gttgatgcgg gcggcgatcg tgccgaagtc
     2401 gcggccgacg tcccactgca catcggagat cggcgtgacc agggcgagga tgtcgtcggg
     2461 gcgggcgctg gtggtgaagt cgagatcggt gaggtcgcgg ttcaggaatg cgtcgcgtac
     2521 cgatccgccg acgagagcga gctcgtgtcc cgccgcggag aaggcggcag cgagtcggga
     2581 gacggcagca gaatcggcga gttcgccgag tcgttcgact gcccttgcga cgctctccat
     2641 gagacccaag cttgtcacag ggccattcga gtgaccggac tcccagaagc gagtgaatag
     2701 actttgggca tgctggccga gcgattcacg ccgctgcgtg ctgcactggc gctgctcctc
     2761 gggctagcgg gagccgtgac cggtgttgcc atcgcggccc ccgcggcgac ctcggtcgga
     2821 cccgacggca ccgtcgccct caccgtggtc gtgccgctgg ccgtgcccga gagcgacgcc
     2881 ggattcatcc cggctgatgc actcgaccag tacacgcgac ccacgggact tctgacccgc
     2941 gagctcgacg cgatcatcga ccgtcccgtg gtgatcgcga tcgatccgcg tatcatcgcg
     3001 tcgatccgga tcctgggctc gtcggcgccg gcgtcggcca tcgaatggct gagccgcctc
     3061 gaatcggcga gcaatgagac cttcgcgctg acctacgccg actccgacat cactctcggt
     3121 cttcaggcgg gtagccctcg agtgctgcag cccgagtcct tcgacttcgc catcgatccc
     3181 tcgctgttcg ccgaggccgc cgctcaaacc cccgccccga ctccgagtcc ggatgccacg
     3241 gcgcccggtc tgccgaccag cgagtcactg ctggcctggc cctacaccct cgagtcgatc
     3301 gcctggccgc gggacggcac cgtcgtcacc gccgacctct cggcgatcac ggccagcggc
     3361 tacacgacga cgatcctgtc gtcggccaac gtctcccgtg acgaggggtc cgcgctggtg
     3421 agggtggacg gcggcaccgc ggcgatctcc gacgatgcgg tgtcgggcgc gcttcgcacg
     3481 gccgtggcat ccgtgtctcc agaggactgg gtgtcggcga tggccaccgt gggcagcgct
     3541 ctggatgccg cgcccgcgac ggtgctcgcc acgctcgacc gcaccgttcc gatcaccgga
     3601 acccacctcg ccgagacgct caccaccttg caggccgacc cgcgcgtgag gctcaccggc
     3661 ctgtcgcagc tcctggccgg ttcccctgac gaggcgaccc tcgtcgactc gcctcagccc
     3721 gccgcgagcg ttgagcggat gaccatgctg ctcgacgccg agtcggcgga gatcaggttc
     3781 gcgaccgtcg cggagtcgcc gagcgccatc acgtcacctc gccgcctcca cctcctcgcc
     3841 ctgctatcga gcgcctggct gcgcaatccc gcgggctggc tcacggcgac cgatgcgttc
     3901 ctcacagact cggacgacct gagagcgtcg gtgcaggtcg tcgcctacag cagcttcaat
     3961 ctgctcgccg acagcgcctc actgcccatc tcggtgagca actccctcga ccaggccgtg
     4021 acggtgtaca tcaccgtgcg gcccgaaaca gcactgctgg cagtcaccga ctcgcgcgtg
     4081 gagctcgtgg tggagccgga ctcgcaggcc aagggtcaga ttcccgtgca ggcggtgtcc
     4141 aacggaacgg ttcaggtgct catcaccctc gccagcggag ccggcgtgcc gatcggcgct
     4201 cccgcgaccg cggagatcaa cgtgcaggcg ggctgggaga cgcccatcgt gctggtggtc
     4261 ggcatcatcg tggtgctggt gttcggcggg ggaatcgtgc gcaacatcct gcgcaggagg
     4321 aagccggcgc gaccggtcga gccgacggat gactgaccgc ccgagcgacg aggtcgatgg
     4381 ggcctccgcg atcgggcgtg ccagcgcggc gctcgcctcg ggcacgatcg tgtcgcgcat
     4441 cctcgggttc gtcaacgccg cggtgctcgc cgcagcgatc ggttacatcg gcggcgcagc
     4501 tgacgccttc acgctggcaa cccagattcc gaacagcatc tacgcgctca tcgcgggagg
     4561 gctgctgagc gcggttctcg ttccgcagat cgtgcgggcg ggcatccagg ccgatggcgg
     4621 tgctgccttc atcaataagc tggtgacact cggcaccgtg gtcttcgtga tcgtcgccgc
     4681 ggccgccacc atcgctgcgc cgtggttggt gagcctgtac gcgcagtcgg gcacgaaggg
     4741 attcggcccc gacgaactcg cgctcgcgac ggcgttcgcc tactggtgcc tgccgcaggt
     4801 gctcttctac gccctctaca gcctgctcgg gcaggtgctc aatgcgcgcc gggtgttcgg
     4861 tcccttcacc tgggcacccg cgctcaacaa cgtgatcgcc atcacgggtg tgtcgatctt
     4921 catcgccctg ttcggtgcct tccccgccaa tcaggatgcc tcggtctgga cgcccacgat
     4981 gatcgccgta cttggcggca ccgccaccct cggcatcgca gcccaggcgt tcatcctcct
     5041 gttcttctgg cgacgcgcgg gactgaccta ccgcccggac ttccgctggc ggggcgtggg
     5101 cctgggcagc accgggcgag cagcagcctg ggtgttcggc atgatcctca tcacccagct
     5161 cgccgccata gtggagaccc gcgtggccac cggcgcctcg ggcgacgcct cgatcggtgc
     5221 gctgaagtac gcgtggctga tcttcatgct gccgcactcc atcgtgaccg tctcgatcgc
     5281 caccgcctac ttcacgcgga tgagcaccca cgcccgcggt ggtgacctcg cgtcgattcg
     5341 g
//
LOCUS       DAOSOU010000153         5340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15488_length_5340_cov_4.617408, whole genome shotgun sequence.
ACCESSION   DAOSOU010000153 DAOSOU010000000
VERSION     DAOSOU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15488_length_5340_cov_4.617408"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..3431)
                     /locus_tag="PLW15_08585"
     CDS             complement(57..3431)
                     /locus_tag="PLW15_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOT34488.1"
                     /translation="MSNFDFLRAGWPQIAEEARRAEHYTNGDPRSSVFYSRRAIELMV
                     HWLYQVDDTLTKPYKDDLAALLYEPTFKRLVGPAIHTKLDLIRKQGNYAVHRPAPFTP
                     KDSLPVVRELFHVMVWVATRYALTPEERPAPGIPFDAAALPTPQTGVVAKTMAQLKKL
                     NDELDAKDAALAKAQDANEALEAQIAALREEVALAKAANATVPDTHDYREDETRDLFI
                     DVLLREAGWKLDQARDREFPVTGMPNNSEGGKGFVDYVLWGDDGLPLGLVEAKRTRKD
                     AHAGQQQAKLYADRLEAVYGQRPVIFYTNGYEHFLWDDASYPPRRVDGFYTRDQLELL
                     IQRRTTRKPLASVEVNPSIVERYYQRRAIAKVAEVFENKERKALLVMATGSGKTRTVI
                     ALADVLIRANWAKRILFLADRVALVNQAANAFKQQLPDSAPVNLVTDKDSEGRVYVST
                     YPTMMGLINPDTNGVRRFGPGHFDLVIIDEAHRSVYQKYGAIFDYFDSLLVGLTATPK
                     DEIDHNTYGLFNLEDGVPTDSYDLTDAIEEGYLVPPVGRSIATKFVREGIRYDDLSES
                     EKQEWDLLEWEDGLIPSEVDSSAMNTWLFNKDTVDTVLETLMTEGRMVAGGDRLGKTI
                     IFAKNNDHAIFIAERFNENYPQYKGEFARMITYQQTYNQSMIDDFSKPESSPHIAISV
                     DMLDTGIDVPEVVNLVFFKPVHSKTKYWQMVGRGTRLRPDLYGPGEHKKDFVIFDVCQ
                     NIEYFNQDLPSAGTSATPSLRSRLFTSRLELLAAIDAAPDDAELSALREGIAARLHEQ
                     VSGMSLDNFLVRRSRRAVERFAQGSSWLSISGDDFTEAGTLADLPSAIDVQDTDELAK
                     RFDLLALRAQLGVLAADPGLAAAKERMRAIASALGEQRGIPVIDNNIQLIEAVAGEDW
                     WVDVTVPMLELVRIRLRELVKLIDSSVRTVVYTDFKDTAGESAIVEIRKVAVGVDRAR
                     FREKAIAFLREHENDLAVSRLRKGLPLAPGDLQQLENIMIENGLDATQVRDAATAAHG
                     LGLFVRSLVGLDRAAATDALSSFTAGLTLTANQQSFVGLMVEQLTQRGIVEPELLFAA
                     PFTDVAPTGPVALFGDAKVNELVAVLRRIRETAEVG"
     gene            complement(3486..4754)
                     /locus_tag="PLW15_08590"
     CDS             complement(3486..4754)
                     /locus_tag="PLW15_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT34489.1"
                     /translation="MSYQRRLIDDELDELLPGLAAISLDGPKGVGKTATASQRAATIL
                     RMDSSADAELIRAAPDQLRTAARPVLIDEWQRLPEVWDAVRRLVDDDRAGGQFLLTGS
                     AAPAGANIHSGAGRIVSLRMRPLSLAERGVETPTVSLAALATQGVTPIDGDTAMGLSD
                     YVAEIMASGLPGIRGLGPRQRREQLDGYIARVIDKEFAEQGLRVRRPATLRAWLQAYA
                     AATASTASYTTILDAATPGQADKPAISTTTTYRDVLSQLWLLDEVPAWSSIGAQFTRL
                     GSTAKHFLADPALAARLLQLRDDDVIDAARGTVIGPQAGGILGRLFEALVALSLQTYS
                     QVGEYSLSHLRTRNGDHEVDFIVHRGAACVGVEVKLGASVDDTDVQHLLWLKQQLGED
                     LRDMVVVNTGSRAYRRADGVAVIPAALLGP"
     gene            4937..>5340
                     /locus_tag="PLW15_08595"
     CDS             4937..>5340
                     /locus_tag="PLW15_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34490.1"
                     /translation="MSDSTTPEPTPTPHAAAVGAPPKRSRLPLAALIVGIVAIVFAII
                     PGLSFVAFLPALTALGLGIAGLISKAPARGMALTGVILGPLALLVGIVVSVSVIVGGV
                     ASSVDQVKPQPHVERPDKPATQTPKPEAPAEEG"
BASE COUNT          853 a         1744 c         1770 g          973 t
ORIGIN      
        1 ctctctttat ccgcggccgt tagacggcaa ccttgggagt ttagcagaga gacggcctac
       61 ccaacctccg ccgtctcgcg aatccgccgc agcaccgcca ccagctcgtt cacctttgca
      121 tcaccgaaca gagcgaccgg ccccgtaggt gcgacatcgg tgaacggtgc cgcgaagagc
      181 aactccggct ccacgatccc ccgctgcgtg agctgctcca ccatgagtcc cacgaacgac
      241 tgctggttcg cggtcagggt gaggcccgcg gtgaacgacg agagcgcatc cgtggctgcg
      301 gcgcggtcca ggccgacgag ggaacgcacg aacagaccga gcccgtgggc ggcggtcgcg
      361 gcatcccgaa cctgggtcgc gtcgaggccg ttctcgatca tgatgttctc gagctgctgc
      421 aggtcgccgg gggcgagcgg cagacccttg cgcaggcgcg agaccgcgag gtcgttctcg
      481 tgctcgcgga ggaacgcgat cgccttctcg cggaagcgcg cgcggtccac gccgaccgcg
      541 accttgcgga tctcgacgat cgcagactcc cccgcggtgt ccttgaagtc ggtgtagacg
      601 acggtgcgca ccgacgagtc gatcagcttc acgagttccc gcagccggat gcgcacgagc
      661 tcgagcatcg gcaccgtgac atccacccac cagtcctcgc cggcgacggc ctcgatcagc
      721 tggatgttgt tgtcgatcac cgggatgccc cgctgttccc ccagcgcact ggcaatggcg
      781 cgcatgcgct ccttggcggc ggctaaaccg ggatccgccg caagcactcc cagctgtgcc
      841 cgcagcgcga ggaggtcgaa gcgcttcgcc agttcatcgg tgtcttgcac gtcaatggcc
      901 gacggcaggt cggcgagggt gccggcctcg gtgaagtcgt ctccgctgat cgagagccac
      961 gacgacccct gagcgaaccg ctccacggcc cgccgcgagc gccgcacgag gaagttgtcc
     1021 aggctcatgc ccgagacctg ttcgtggagg cgagcggcga tgccctcgcg cagtgcggag
     1081 agctcagcgt catccggggc ggcatcgatc gcggcaagca gctcgagacg tgacgtgaac
     1141 aggcgcgacc ggagcgacgg cgtggccgag gtgcccgccg aaggaaggtc ctggttgaag
     1201 tactcgatgt tctgacacac atcgaagatc acgaagtcct tcttgtgctc ccccgggccg
     1261 tacaggtcgg ggcgcaggcg agtaccgcgg ccgaccatct gccagtactt cgtcttggag
     1321 tgcacgggct tgaagaacac caggttcacg acctcgggca cgtcgatgcc ggtgtcgagc
     1381 atgtcgacgg agatggcgat gtgcggcgac gactcgggct tggagaagtc gtcgatcatg
     1441 ctctggttgt acgtctgctg gtacgtgatc atgcgcgcga actcgccctt gtactgcggg
     1501 tagttctcat tgaaccgctc cgcgatgaag atggcgtgat cgttgttctt ggcgaagatg
     1561 atcgtcttgc cgagccggtc gccgcccgcg accatccgcc cctcggtcat cagggtttcg
     1621 agaaccgtgt cgaccgtgtc cttgttgaac agccaggtgt tcatcgcgct ggaatcgacc
     1681 tcgctcggga tgagcccgtc ctcccactcg agcaggtccc attcctgctt ctcgctctcg
     1741 ctgagatcgt cgtagcggat gccctcccgc acgaacttcg tcgcgatcga gcggcccacc
     1801 ggcggcacga gatagccctc ctcgatcgca tcggtgaggt cgtaggagtc ggtgggtacg
     1861 ccatcctcga ggttgaagag gccgtaggtg ttgtggtcga tctcgtcctt cggggtggcc
     1921 gtcaggccca cgagcaggga gtcgaagtag tcgaagatcg cgccgtactt ctggtacacc
     1981 gagcggtgcg cctcatcgat gatcaccagg tcgaaatggc ctggaccgaa gcgcctgaca
     2041 ccgttcgtgt ccgggttgat gaggcccatc atcgtcgggt aggtcgagac gtagacgcgc
     2101 ccctcggagt ccttgtcagt cacgaggttc accggcgccg agtcgggcag ctgctgcttg
     2161 aatgcgttgg ctgcctggtt gacgagcgcc actctgtcgg cgaggaacag gatgcgcttc
     2221 gcccagttcg cgcggatcag cacgtcggcg agtgcaatca ccgttcgcgt cttgccggaa
     2281 cccgtggcca tcaccagcag cgccttgcgc tccttgttct cgaacacctc agcgaccttc
     2341 gcgatcgcac gccgctggta gtagcgctcg acgatcgagg ggttcacctc gacactcgcg
     2401 agcggcttgc gggtggttcg gcgctggatc agcagctcga gctggtcgcg ggtgtagaag
     2461 ccgtccaccc gccgcggcgg gtagctcgcg tcatcccaga gaaagtgctc gtagccgttg
     2521 gtgtagaaga tcaccggacg ctgcccgtac accgcctcga gccggtcggc gtagagcttg
     2581 gcctgctgct gaccggcgtg cgcgtccttg cgggtgcgct tggcctcgac gagtccgagt
     2641 ggcaggccgt cgtcacccca gagcacgtag tcgacgaagc ccttgccgcc ctcggagttg
     2701 ttgggcatgc ccgtgaccgg gaattcgcgg tcgcgagcct ggtcgagctt ccagcctgcc
     2761 tcgcgcagta gcacgtcgat gaacaggtcg cgggtctcgt cttcgcggta gtcgtgcgtg
     2821 tcgggaactg tcgcgttggc ggccttcgcg agcgccactt cctcgcgcag cgcggcgatc
     2881 tgggcctcga gggcctcgtt ggcgtcctgc gccttggcga gcgcggcatc cttggcatcg
     2941 agctcgtcgt tgagcttctt gagctgcgcc atggtcttgg cgaccacgcc cgtttgcggt
     3001 gtaggcagtg cggccgcatc gaacgggatg cccggggctg gcctctcctc gggcgtgagc
     3061 gcgtagcgcg tagccaccca caccatcacg tggaacagct cccgcaccac cggcaggctg
     3121 tccttcggtg tgaacggtgc tggccggtgc acggcgtagt tgccctgctt gcggatcagg
     3181 tcgagtttgg tgtggatggc cgggccgacg agtcgcttga aggtgggctc gtagagcagc
     3241 gcggcgaggt cgtccttgta gggcttggtg agcgtgtcat ccacctgata gagccagtgc
     3301 accatgagct cgatcgcccg acgcgagtag aagaccgacg agcgcgggtc accgttggtg
     3361 tagtgctcgg cgcggcgcgc ctcttcggcg atctggggcc agccggcacg caggaagtcg
     3421 aagttgctca ttcgatcctt cctcagtgct tgccgaacag attatcgggc ctggggtgtc
     3481 atcggtcacg gacccagcag ggcggccggt atcacagcga ctccatcggc gcggcggtag
     3541 gctcggctgc cggtgttgac gacgaccatg tcccgcaggt cttcgccgag ctgctgcttc
     3601 agccacagca ggtgctgtac gtcggtgtcg tccacactgg cgcccaactt gacctccacc
     3661 ccgacgcacg ccgcgccgcg gtgcacgatg aaatccactt cgtggtcgcc gttgcgcgtg
     3721 cgcaggtgac tgagcgagta ctcgcctacc tgcgagtagg tctggaggga aagggcaacg
     3781 agagcctcga acaggcggcc gaggattccg ccggcctgag gaccgatcac tgtgcccctc
     3841 gcggcgtcga tcacatcgtc atcacgcagt tgcagcaatc gcgccgcgag cgccgggtcc
     3901 gcaaggaaat gcttggccgt gctccccaag cgagtgaact gcgccccgat cgatgaccac
     3961 gcggggacct cgtcgagcaa ccagagttga gagagcacat cgcgataggt ggtggtggtc
     4021 gagatcgctg gcttgtcggc ctgaccgggg gtcgcggcat ccagaatcgt cgtgtagctg
     4081 gccgtggatg ccgtggctgc ggcgtacgcc tgcaaccacg ctcgcagagt ggctgggcgc
     4141 cgcacccgga ggccttgttc ggcgaactcc ttgtcgatca cgcgcgctat gtacccgtcc
     4201 agctgctccc gcctctgtcg cgggccgagt cctcgaattc ccggcagacc ggacgccatg
     4261 atttctgcga cgtagtcgct gagccccatg gcggtgtcgc cgtcgatagg cgtgactccc
     4321 tgggtcgcca acgcagcgag ggacacggtg ggcgtttcca caccgcgttc ggcgagggac
     4381 agaggacgca tccggaggct cacgatgcga ccggcgcccg agtggatgtt cgctccggcg
     4441 ggtgcggcgc tgccggtgag caggaactgc ccgccagcgc gatcatcgtc caccagacgc
     4501 cgcaccgcat cccatacttc gggaagccgc tgccactcat cgatcagcac gggccgcgct
     4561 gccgttcgca gctggtctgg ggccgcacga atcagctcgg cgtcggctga cgagtccatc
     4621 ctcaggatcg tggccgcgcg ctgactcgct gtggcggtct tgccgactcc cttgggccca
     4681 tcaaggctga ttgcggcgag tcccggcaac aattcgtcga gctcatcgtc gatcaggcgg
     4741 cgctggtagc tcatccagac tctcatcctg tgtttagcag ttcttctacc atcagattag
     4801 cagctcatct atctcatgaa tagcagctca ttgatctgga cgtgacgaaa tgtcggaggc
     4861 ctctggtcaa ctgtccgact tgcctccggc aggttccgcg gcgagcgccg cgggcaacag
     4921 caagcaagga gacatcatgt ctgacagcac cactcccgaa cccaccccga cccctcacgc
     4981 tgcggctgtg ggagcgcccc cgaagcgcag ccgcctaccg ctggccgccc ttatcgtcgg
     5041 catcgtcgcg atcgtgttcg cgatcattcc cggactctcc ttcgtggcgt tcctgcccgc
     5101 tctcaccgcg ctcggactcg gtattgcggg cctcatctcg aaggcaccgg cgcgcgggat
     5161 ggcgctcacg ggcgtgatcc tggggccgct cgcgctgctg gtgggcatcg tagtgagcgt
     5221 gtccgtgatc gtcggtggcg tcgcatccag cgtcgaccag gtgaagcccc agccgcacgt
     5281 cgagaggccg gacaagcccg ccacccagac gcccaagccg gaagctccgg ccgaggaggg
//
LOCUS       DAOSOU010000154         5323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15551_length_5323_cov_5.470767, whole genome shotgun sequence.
ACCESSION   DAOSOU010000154 DAOSOU010000000
VERSION     DAOSOU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15551_length_5323_cov_5.470767"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1083
                     /locus_tag="PLW15_08600"
     CDS             1..1083
                     /locus_tag="PLW15_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOT34491.1"
                     /translation="MSTAAAKHQPTKRQIRRWRQYLADERAEAAVYRDLASRRKGEER
                     EILLALAEAEGRHEQHWLDLLGDDVGAPRRGDLRTRLLGFLARRFGSVFVLALAQRAE
                     TRSPYDDDADATDAMAADERIHGEVVRGLAIRGRMRISGTFRAAVFGANDGLVSNLAL
                     VLGIGATGVSTATVLFAGLAGLLAGALSMGAGEYVSVRSQRELIDAARPDPASQSVLP
                     DLDVDANELALVYRARGMTQVAAEARATEVLDSLHLTPELTAADPEETTDTVGTAWGA
                     AISSFCFFASGALIPVLPYLFGATGVTAIVIASVLVGLALLATGAVVGLLSGASPLKR
                     ALRQLAIGFGAAAVTYLLGLWFGATL"
     assembly_gap    1203..1302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1303..>2588)
                     /locus_tag="PLW15_08605"
     CDS             complement(<1303..>2588)
                     /locus_tag="PLW15_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT34492.1"
                     /translation="TAAAVVALGLDAATLGALSDDALLAAHTLLTDHRRHADTIAAHL
                     AGEIARRSTRDDGYAGLSQRKGFGSATGLLQSLSPVTASEASQLISAGVLLADPPSTL
                     WEGALAQALSAGLVSVAATDAIRRGLTPVAETAPAEELLAECQRLLSRSPQLSIDELR
                     REARSARDRIDEAGIARRERQRRDLRYLKRWVRDDGMYQGAFLLDPEAGLHLFTALDA
                     IMAPRRAVRFVDATAPDEAVLTDTRTDDQLLADALTEMVRLAVDVDPGTLFGTHRPAV
                     RVIVTETTLRGPGGHGYLESDPQPVSRDTVDRHLCDTGFIGIKFDRKGQILDLGRTER
                     LFVSAQRLVISVRDGGCLFLHCTKPPSACEVHHITDWRHGGKTDVRDGVLLCQHHHML
                     LHNNKWKILREHDRYWLKPPVTEDPDQRLRPLPTKSP"
     assembly_gap    2589..2688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2872..3573)
                     /locus_tag="PLW15_08610"
     CDS             complement(2872..3573)
                     /locus_tag="PLW15_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34493.1"
                     /translation="MGFMDMFRPVNAPIAGGVAGTAQVVSASVAAGTGVFQKCSLHLV
                     INAPGLTPTAVEFTGAINVAKWPTVGGILPVSVDPNDPRHYAILWNQVPSRNEVAKTS
                     AEGVAAALRGEPDALAKLVGGGMLGDTVQIIGDPSHITPEAKAKLAAFGIDIDALIAG
                     AARAGGGAQSAGAAQSYGGATPFTVLPGLGSAGGSTGGSVDIVSKLERLQVLRSNGTL
                     TDAEFESGKKMILGG"
     gene            3737..4456
                     /locus_tag="PLW15_08615"
     CDS             3737..4456
                     /locus_tag="PLW15_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT34494.1"
                     /translation="MTSPDPLTERNGVLLDRRVLVVEDDPTVREVAVSYLEAAGFIVG
                     QATDGFAALEAVASGGHDLIVLDRMLPGIDGAEVCRRIRATTAVPIIMLTALGSTEDR
                     ITGLEAGADDYLTKPFSPRELVLRVQSILRRSVTDLTPEAAFELGGFVLDPSARVVSK
                     NGQVLTLSAREFDLFAFLLKHPSQAFSREQLLKSVWHWDFGDLSTVTVTVRRVREKIE
                     DDPTNPTILETVWGVGYRLTI"
     gene            4464..>5323
                     /locus_tag="PLW15_08620"
     CDS             4464..>5323
                     /locus_tag="PLW15_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT34495.1"
                     /translation="MLSLVDLATIVAIALACALAVGGLGLVVLRLARGASMRLQLFVV
                     VVSAILSVTLGMIAASGAMYISGHDLVVMFSVTAASALVSIGMALVLGRAFTRNSNRL
                     RMLARALGDGERLDAQPRRTDTTEFAGLAAELAATSERLAQARDEVAAMDASRRQLVA
                     WISHDLRTPLAGLRAMAEALEDDLAADPQRFHRQMRSQVDHLSAMVDDLFELSKITSG
                     TLVLDLEPLSLYDLASDAVAELGTLARSRDLTLLAPEGGDLMVVGDHRELARVVGNLL
                     MNAIQHSPSGG"
BASE COUNT          754 a         1690 c         1817 g          862 t
ORIGIN      
        1 atgagcaccg ctgccgcaaa gcaccagccg acgaaacgcc agattcgccg ctggcggcag
       61 tacctcgccg atgagcgcgc cgaggccgcc gtgtaccggg atctcgcatc ccgacgcaag
      121 ggcgaggagc gcgagatcct gctggcgctc gccgaagcgg agggccgcca tgagcagcac
      181 tggctcgacc tgctgggcga cgacgtgggt gctccgcggc gtggtgacct gcgcacccgg
      241 ctgctcggct tcctggcgcg acgattcggc tcggtgttcg tactggcgct cgcgcagcgt
      301 gccgagacac ggtcgcccta cgacgatgac gcggatgcga cggatgcgat ggccgccgac
      361 gagcggatcc acggtgaggt ggtgcgcggc cttgccatcc gcggccggat gcgcatctcg
      421 ggaacgttcc gggcggcggt attcggcgcc aacgacgggc tggtctccaa cctcgccctc
      481 gtgctgggta tcggagcgac tggcgtcagc accgcgacgg tgctgttcgc gggacttgcc
      541 ggtctgctcg ccggcgccct gtcgatgggt gcgggggagt acgtctcggt gcgctcgcag
      601 cgcgaactga tcgatgccgc acggcccgat cctgcgtcgc agtcggttct gcccgacctc
      661 gacgtcgacg ccaacgagct cgcgctggtc taccgcgccc gcggcatgac gcaggtggcc
      721 gcggaggctc gcgcgaccga ggtgctcgac tcgctccacc tcactccgga actcaccgcc
      781 gccgaccccg aggagaccac cgacaccgta ggcacggcgt ggggtgcggc gatctcgagc
      841 ttctgcttct tcgcctcggg cgcgctgatc ccggtgctcc cgtacctgtt cggcgcgacc
      901 ggtgttaccg cgatcgtgat cgcgtcggtg ctggtgggtc tcgccctgct ggcgaccggg
      961 gccgtcgtcg ggttgctgtc gggggcatcc cctctgaagc gtgccctgcg ccagctggcg
     1021 atcggattcg gagctgcggc cgtcacctac ctgctcggcc tctggttcgg tgcgacgctg
     1081 tgaccgagga aaccctgtga ccgagcgaac cctgtgaccg agcgaaacgt atgagcgagc
     1141 aaaccccgtg accgagctgc acctcggtga gcgaggaaac tcctgtgacc gagcaaaccc
     1201 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggactctt cgtgggcagc
     1321 ggtctgagcc gctggtcggg gtcttcggtg acgggtggct tcagccagta tcggtcgtgt
     1381 tcgcggagga tcttccactt gttgttgtgc aggagcatgt ggtgatgttg gcaaagcaga
     1441 acaccgtctc ggacgtcggt cttgccgccg tgtctccaat cggtgatgtg gtgcacttca
     1501 caagcggaag gcggcttggt gcagtggagg aagagacatc cgccgtctcg gaccgagatc
     1561 accaaccgtt gggcggagac gaagagccgc tctgtgcggc cgaggtcgag gatctgtcct
     1621 ttgcggtcga acttgatgcc gatgaatccg gtgtcgcaca ggtgacggtc gacggtgtca
     1681 cgggagaccg gttgggggtc gctttcgagg tagccatggc cgccgggccc gcggagggtg
     1741 gtttcggtga cgatcacccg cacggctggt cggtgggtgc cgaacagggt accggggtca
     1801 acatcgacgg ctaaacggac catctcggtg agcgcatcgg cgaggagttg gtcgtcggtg
     1861 cgggtgtccg tgaggacggc ctcgtcgggc gccgtggcat ccacgaatct gacggcacgg
     1921 cggggcgcca tgatcgcatc caacgcggtg aacaggtgga gaccggcttc ggggtcgagc
     1981 aggaacgcgc cctggtacat gccgtcgtcg cgcacccagc gcttgaggta gcgcaggtcg
     2041 cgacgctgtc gctcccgccg agcgatgccg gcctcgtcga tgcggtcacg tgcggatcgg
     2101 gcttcacgcc gcaactcatc gattgacagt tgcggcgagc gcgacagcaa ccgctggcac
     2161 tccgcgagca gttcctcggc cggtgccgtc tcggcgacgg gggtgagtcc gcggcggatc
     2221 gcatccgtcg cggctaccga caccagccca gccgatagtg cctgcgcgag ggcgccctcc
     2281 cacagcgtgc tcggcggatc tgccagcaac acgcccgccg agatcagttg actcgcctca
     2341 ctggcggtga ccggcgacag actctggagc aaccctgtgg cacttccgaa gcccttgcgc
     2401 tgagacaacc cggcataccc gtcatcccgt gtggagcggc gggcgatctc cccggcaagg
     2461 tgggcggcga tcgtgtcagc gtggcggcgg tggtcggtga gcagtgtgtg ggcggccagc
     2521 aatgcatcgt cactgagtgc cccgagagtc gcggcatcca gaccgagggc aacgaccgca
     2581 gcggcggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg atgcggctgt
     2701 gcgggtgtgg tggggaaggg gcttgcgcgg gagtcgggtg cgcgggatgc ggctgtgcgg
     2761 gtctggtggg gaaggggctt gcgtgggagt cggcgcggga gtcggctgtg ccggcgaggg
     2821 agcagaagat gggctcatgc ggattccagg ggggtggagg ggaggaagcc cctaaccccc
     2881 cagaatcatc ttcttgccgg actcgaactc cgcgtcggtg agcgtgccgt tgctgcgcag
     2941 cacctgcagt cgctcgagct tgctgacgat gtccaccgag ccgcccgtcg agccgcccgc
     3001 cgagccgagc cccggcagca cggtgaacgg tgtcgcaccg ccatatgact gagcagcgcc
     3061 agccgactga gcaccaccac cagcccgcgc tgcacccgcg atgagcgcgt cgatgtcgat
     3121 gccgaaggct gcgagcttcg ccttcgcctc gggtgtgatg tggctggggt cgccgatgat
     3181 ctgcacggta tctcccagca tgcccccgcc cacgagtttg gcgagcgcgt caggctcccc
     3241 gcgcagtgcg gcggcgacgc cctcagccga tgtcttcgcg acctcgttgc ggctgggaac
     3301 ctggttccac agaatcgcgt agtgccgtgg gtcgttcggg tcgaccgaga ccggcaggat
     3361 gccgccgacg gtcggccact tcgcgacatt gatggcaccc gtgaactcga ctgccgtggg
     3421 ggtgaggccc ggcgcgttga tgacgaggtg cagcgagcac ttctggaaca ccccagtgcc
     3481 ggcggcgacc gacgcggaga ccacctgcgc ggtgcccgcg accccgcccg cgatgggggc
     3541 gttgaccggc ctgaacatgt ccatgaatcc catgcgacgc tccttcggtt gcctcgaaga
     3601 ctacccgtga cggatgcccg tgcagaggcc tcacgaagcc agagcaccga gccccaaccc
     3661 ttacgaaccg cagacgatct cccgatcgcc cctgttcgcc ctgacgcgac cgcgcgcgcg
     3721 aacctatcct tcagttgtga cttctcccga ccccctcacc gagcgcaacg gcgtgctcct
     3781 cgaccgccgc gtgctggtgg tcgaggacga ccccacggtg cgcgaggttg cggtgagcta
     3841 cctggaggcc gcgggattca tcgtggggca ggcgaccgac gggttcgcgg cgctggaggc
     3901 cgtcgcatcc ggaggtcacg acctgatcgt gctcgaccgg atgctgcccg ggatcgatgg
     3961 cgccgaggtg tgccgacgca tcagggcgac cacagcggtg cccatcatca tgctgaccgc
     4021 gctgggctcg acggaggatc gcatcaccgg gctcgaggcc ggcgccgacg actacctcac
     4081 gaagcccttc tcgccgcgcg agctcgtgct gcgggtgcag tcgatcctgc ggcgcagcgt
     4141 gaccgatctc acgccggagg cggcgttcga gctgggcggg ttcgtgctcg acccgtcggc
     4201 tcgggtggtg tccaagaacg ggcaggtgct caccctctcg gcgcgcgagt tcgatctgtt
     4261 cgcgttcctg ctcaagcatc cgagtcaggc gttcagccgc gagcagctgc tgaagtcggt
     4321 ctggcactgg gacttcggcg acctctcgac cgtgacggtc accgtgcgtc gggtgcgcga
     4381 gaagatcgag gatgacccga ccaaccccac gatcctcgag accgtgtggg gcgtgggcta
     4441 ccgcctgacc atctgaggat ctgatgctct cactcgtcga cctggcgacc atcgtcgcga
     4501 tcgccctggc ctgcgctcta gccgtcggcg ggctgggctt ggtggtgctg cgtctggcgc
     4561 gcggcgcatc gatgcgtctt cagctcttcg tggtcgtggt ctcggcgatc ctctcggtga
     4621 cgctcggcat gattgcggcg tctggcgcga tgtacatctc cggccacgac ctcgtggtga
     4681 tgttctcggt gacggcggca tccgcgctgg tctccatcgg catggcgctg gtgctgggcc
     4741 gcgcgttcac ccgcaacagc aaccggctca ggatgctcgc ccgcgcgctc ggcgacggtg
     4801 aacgactcga cgctcaaccc cgccgcaccg acaccaccga gttcgctggg ctcgccgccg
     4861 agcttgccgc gaccagcgag cggctggctc aggcccggga cgaggtggcg gcgatggatg
     4921 cgagccgccg gcagttggtg gcctggatct cgcacgacct tcgcaccccg ctggcggggc
     4981 tgcgcgccat ggcggaggcg ctcgaggacg atctggccgc cgacccgcaa cggttccacc
     5041 ggcagatgcg ctcccaggtc gatcatctct cagcgatggt cgacgacctg ttcgagctgt
     5101 cgaagatcac ctcgggcacg ctcgtgctcg acctggaacc cctgtcgctc tacgacctgg
     5161 ccagcgacgc ggtggctgag ctgggcacac tggcgcgctc ccgcgatctc accctgctgg
     5221 ctcccgaggg cggcgacctc atggtggtcg gggatcatcg cgaactcgcg cgcgtggtgg
     5281 gcaacctgct catgaacgcg atccagcatt cgccgtcggg cgg
//
LOCUS       DAOSOU010000155         5263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15795_length_5263_cov_6.357911, whole genome shotgun sequence.
ACCESSION   DAOSOU010000155 DAOSOU010000000
VERSION     DAOSOU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15795_length_5263_cov_6.357911"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLW15_08625"
     CDS             complement(<1..1008)
                     /locus_tag="PLW15_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT34496.1"
                     /translation="MSRRREHLLAALLGVLAAAVTVALGDLIAQFVDAGSSPLFAVGA
                     WVIDIVPGWVKETAIALFGTGDKVALLVGLGLLVAILAVAAGILEYRRSPWGVVVLVG
                     VGVVAAGAAVTRAGASVNWILPTAIGVAAGAIVLRMTTDRLRRWTRAIADQTPKADGI
                     YAATSRRGFINAVAGSAVAAVLVGIAGRAMSAATSAANGVREAIALPKPVTSAPPIPA
                     GAELGLRGLSAVVTSNDTFYRIDTALQVPRIDPAQWKLRIVGLVDREIEIGYDELLAL
                     PMTETVATLMCVSNEIGGNLIGNAVWLGYPIRELLKKAGPKAGADMVLSTSQDGWTAS
                     TP"
     gene            complement(1055..1738)
                     /locus_tag="PLW15_08630"
     CDS             complement(1055..1738)
                     /locus_tag="PLW15_08630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT34497.1"
                     /translation="MYRAAKRLCDILIALIALLILSPILVVIMIALLLTGEHEVFYRQ
                     TRIGAGYKTFGIWKFATMLKDSPNIGTGSLTVRGDPRVTVVGRFLRATKINELPQLIN
                     VLTGEMSLVGPRPQMKVDFDLYPPDVRDAIYSVPPGVTGIGSIVFRDEELLLSRPGVE
                     PREFYVDKIAPYKGALELWYVSHRSLALDARLVLLTAWVVLRPESTIVFRSFPDLPQR
                     PDWLIVRNS"
     gene            complement(1741..2961)
                     /locus_tag="PLW15_08635"
     CDS             complement(1741..2961)
                     /locus_tag="PLW15_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase"
                     /protein_id="HOT34498.1"
                     /translation="MNLTTFSVDDFISSAVTGRRSSLFGDDLAAHESQLRAGIEGKSV
                     LVIGGAGTIGSSFIRALLPFHPARLVVVDISENGLTELTRDLRSTAGQYVPADFLTYP
                     ISFGDAVFERMLRAEGPFDIVAHFAAHKHVRSEKDRYSIEAMLENNVLSTARLLDLLL
                     ESPPEHFFCVSTDKAANPANVMGASKKLMEEAVLAYAAELPATTARFANVAFSNGSLP
                     AGFLERLAKHQPLSAPSDVLRYFVSPEESGQLCLLACITGEPGDIFFPRLEQPQMLTF
                     SSIADALLERLGYEAVQCASEDEARALAASLGPEDTRWPVYYFTSDTSGEKGFEEFYT
                     NTETVDWERFTALGVVKGRTSQNVAGIRQLGSDLRGLFAQPELSKEEIVAVLTRLVPT
                     FHHVETGRSLDQKM"
     gene            complement(2958..4169)
                     /locus_tag="PLW15_08640"
     CDS             complement(2958..4169)
                     /locus_tag="PLW15_08640"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT34499.1"
                     /translation="MLIPFSLPLIDDDVIAEVNDALVNTGWLTTGPKVRLLEEELSRM
                     TDRLVVCVNSWTSGAMLVLRWFGVGPGDEVIIPAYTYSATALCAMNLGATVVMVDVSD
                     DFTIATEQIRAAITERTKAIIPVDVAGWPADYDGIRDVLDAPDVRAMFTPASERQAKL
                     GRILVLSDAAHSIGSTYHRRPNGQWADVTVFSFHSVKNITTGEGGAVILNLPAPFDND
                     AERLFLRTFSLNGQTKSAFEKNQSGGWRYDIIDQGLKANMPDVLAAIGLSQIRKYAKE
                     LLPDRRRKFELYQAAFADDSWAILPPYRDAERESSAHLYMLRIAGADEARRDAIIQHL
                     ADAGIGVNVHYIPMAMLTLFRERGYKIGDYPKTYELYANEITLPLYNVLTEAAIAEVI
                     AGVRAAVEDTA"
     gene            complement(4187..5122)
                     /locus_tag="PLW15_08645"
     CDS             complement(4187..5122)
                     /locus_tag="PLW15_08645"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT34500.1"
                     /translation="MTSEATTGMVHTRAPGKINVFLKVGALLEDGYHDVAIAYQAVSL
                     YEDVRAYPADDFSVSVTGSVELSRVPTDARNIAIKAARLLARRTGYRGGVRLEIDKHV
                     PVTGGMGGGSADAAATLLACDTLWGTALPREEMLHLAAELGADVPFAFTGGTAIGTGR
                     GDQLSPALAKGQFQWVLALADFGLSTPEVYSELDRHRDRNSQDIFPAPVAPTVEANVL
                     QALRAGDPHMLADCLYNDLQAPALHLEPSLAAVLELGERNGALAGIVSGSGPTVAFLA
                     ADLDSALELQIALSAAQLTVVRATGPVHGARVVTD"
     gene            complement(5162..>5263)
                     /locus_tag="PLW15_08650"
     CDS             complement(5162..>5263)
                     /locus_tag="PLW15_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HOT34501.1"
                     /translation="AAASARLEAAGIAPTARGEELTVDDFLSVARAG"
BASE COUNT          914 a         1719 c         1775 g          855 t
ORIGIN      
        1 cggggtgctc gcggtccagc cgtcctggct ggtcgacagc accatgtcgg cgccggcctt
       61 gggccccgcc ttcttcagaa gttcgcgaat gggatagccg agccagacgg cgttgccgat
      121 caggttgccg ccgatctcgt tcgacacgca catcagcgtc gccacggtct cggtcatggg
      181 cagcgcgagt agctcgtcgt agccgatctc gatctcccga tcgaccaggc cgacgatacg
      241 cagtttccac tgcgccgggt caatacgggg cacctgcagc gcggtgtcga tgcggtagaa
      301 cgtgtcgttc gaggtgacga cggccgagag cccccgcaac ccgagctcgg cgcccgcagg
      361 aatcggcggg gccgacgtca ccggcttcgg gagcgcgatc gcctcgcgca ccccgttcgc
      421 cgccgaggtc gccgcactca tggcacgccc ggcgatacct acgagcacgg cagccacggc
      481 agaaccggcg actgcgttga tgaaaccgcg acggctggtt gccgcataga tgccgtcggc
      541 cttcggggtc tggtcagcga tcgcgcgcgt ccagcgacgc aaacggtcgg tggtcatccg
      601 cagcacgatc gcgcccgccg ccacaccgat cgcggtcggc agaatccagt tgacggatgc
      661 cccagcgcga gtcaccgcgg ccccggccgc aacgacgccg acgccaacga gcacaaccac
      721 accccacggc gagcgccggt actccaggat gcccgcggcg acggcaagga tggcgacgag
      781 caacccgagc ccaaccagca gcgcgacctt gtcgcccgtt ccgaacagcg cgatcgccgt
      841 ctctttcacc cagccgggca cgatgtcgat gacccaggcg ccgacagcga agagcgggct
      901 gctgcccgca tcgacgaact gggcgatcag gtctccgagg gctacggtga ctgcggcggc
      961 cagcacgccc agaagggcag cgagtaggtg ctcccggcga cggctcatgg gccgatccta
     1021 accggcggcc cgtgatcgcc tgagccgaag aaccttacga gttccggaca atgagccaat
     1081 cgggtcgctg cggcaggtcg gggaacgatc ggaagacgat cgtcgactcg gggcgcagta
     1141 ccacccaggc cgtgaggagc acgagccggg catccagagc cagggagcgg tgggagacgt
     1201 accagagctc gagagcgccc ttgtaggggg cgatcttgtc cacgtagaac tcgcgcggct
     1261 cgacgccggg tcgggagagc agaagttcct cgtcgcggaa cacgatcgag ccgatgccgg
     1321 tgaccccggg cggcaccgag tagatcgcgt cgcgcacgtc cggcggatag agatcgaagt
     1381 cgaccttcat ctgcggccgc ggtccgacca ggctcatctc acccgtgagc acgttgatga
     1441 gctggggcag ttcgttgatc ttggtcgccc gcaggaagcg tccgacaacc gtgactcgcg
     1501 gatccccgcg cactgtgagc gaaccggtgc cgatgttggg gctgtccttg agcatggtcg
     1561 cgaacttcca gatgccgaac gtcttgtagc ccgcgccgat ccgggtctgg cggtagaaca
     1621 cctcgtgctc gcccgtcaac agcagagcga tcatgatcac gacgaggatc gggctgagga
     1681 tcagcaacgc gatcagcgcg atgaggatgt cacagagtct cttggcggcg cggtacatgc
     1741 tcacatcttc tggtcgaggc ttcgaccggt ctcaacgtgg tggaacgtcg ggacgagtcg
     1801 ggtgagcacg gccacgatct cctccttgct cagctccggt tgggcgaaca gcccccggag
     1861 gtcgctgccc agctgacgga tgcccgccac gttctgactg gttcgaccct tcaccactcc
     1921 gagagcggtg aagcgttccc agtccacggt ttcggtgttc gtgtagaact cctcgaaacc
     1981 cttctcgccc gaagtgtcgg acgtgaaata gtacaccggc cagcgggtgt cctccgggcc
     2041 gagggatgcc gcgagcgcac gcgcctcgtc ctcgctcgcg cactgcaccg cctcgtaccc
     2101 cagccgctcg agcagcgcgt cggcgatgga actgaaggtc agcatctgcg gctgctccag
     2161 ccgcgggaag aagatgtcac ccggttcgcc cgtgatgcag gccagcaggc atagctgtcc
     2221 gctctcttcg ggcgagacga agtagcgcag cacgtcgctc ggagcagaca gcggctggtg
     2281 cttggccaat cgctcgagga acccggcggg cagcgaaccg ttggagaatg ccacgttcgc
     2341 gaaacgcgcg gtcgtcgccg gcagttcggc cgcgtaggcg agcacggcct cctccatgag
     2401 cttcttcgag gcgcccatga cgttggccgg gttggcggcc ttgtcggtgg agacgcagaa
     2461 gaagtgctcg ggaggcgact cgagaagcag atcgagcagt cgcgccgtcg acagcacgtt
     2521 gttctcgagc atcgcctcga tcgagtagcg gtccttctcg ctgcgcacgt gcttgtgggc
     2581 ggcgaagtgc gcgacgatgt cgaacggacc ctcggcacgc agcatccgct cgaagaccgc
     2641 gtcgccgaag ctgatcgggt atgtcaggaa gtcggcggga acgtactgcc cggcggtcga
     2701 cctgaggtcg cgagtgagct cggtgagacc gttctcgctg atgtcgacga cgaccagacg
     2761 cgccgggtgg aacggcagaa gtgccctgat gaaggatgac ccgatcgtgc ccgccccgcc
     2821 gatgacgagc accgacttgc cttcgatgcc cgcccgcagc tgcgactcgt gcgccgccag
     2881 atcgtcgccg aacaggctcg agcgccgacc ggtgaccgcg ctggagatga agtcgtcgac
     2941 gctgaaggtc gtcaggttca cgcggtgtcc tccacggccg cgcgaactcc cgcgatcact
     3001 tcggctatgg cggcctcggt gagcacgttg tagagcggca gggttatctc gttcgcatac
     3061 aactcgtagg tcttggggta gtcaccgatc ttgtagcctc gctccctgaa cagggtcagc
     3121 atcgccatcg ggatgtagtg cacgttgacg ccgatgccag catcggcaag gtgctggatg
     3181 atcgcgtcac gccgtgcctc gtcagcccca gcgatacgca gcatgtacag gtgggcactc
     3241 gactcgcgtt cggcgtcgcg gtacggcggc aggattgccc aggagtcatc cgcgaacgcc
     3301 gcctgataga gctcgaattt ccgccggcga tcgggaagca gctctttcgc gtacttgcga
     3361 atctgtgaaa gcccgatcgc ggccagcacg tcaggcatgt tggccttcaa accctggtcg
     3421 atgatgtcgt agcgccagcc accgctctga ttcttctcga acgcgctctt ggtctggccg
     3481 ttgagggaga aggtgcgcag gaagagacgt tcggcgtcgt tgtcgaaggg cgcggggagg
     3541 ttgaggatga cggcgccacc ctcgccggtg gtgatgttct tgaccgagtg gaacgagaag
     3601 accgtgacat ccgcccactg cccgttcggg cggcggtggt aggtagaccc gatcgagtga
     3661 gccgcgtcgg agagcacgag gatgcgcccc agtttcgcct gccgttcgct ggccggcgtg
     3721 aacatcgctc gcacgtcggg tgcgtccagc acgtcccgga tgccgtcata gtcggcaggc
     3781 cagccagcaa catccacggg gatgatcgcc ttggtgcgct ccgtgatcgc ggcgcgaatc
     3841 tgctcggtgg cgatcgtgaa gtcatcgctc acgtcgacca tcacgactgt cgctccgagg
     3901 ttcatcgcac acagggcagt agcgctgtag gtgtacgcgg ggatgatcac ctcgtcgccc
     3961 gggcccacgc cgaaccagcg aagaacaagc atcgccccac tcgtccagga gttcacacat
     4021 accaccaggc ggtcggtcat ccgcgagagt tcctcctcca acagccgcac cttcggaccg
     4081 gtggtgagcc acccagtatt cacgagtgcg tcgttcacct cggcgatgac gtcatcgtcg
     4141 atgaggggca gcgagaaggg aatgagcata tttctaattt agtgcctcag tcggtcacaa
     4201 cgcgcgcacc gtgaacggga ccagtggccc gcaccacggt gagctgcgcc gcgctcagcg
     4261 cgatctgcaa ctcgagtgcc gagtccaggt cagccgcgag gaatgcgaca gtcggacccg
     4321 aacccgacac tattcccgcg agcgccccgt tgcgctcacc tagttcgagc accgccgcga
     4381 gcgatggttc gaggtgcagc gctggggcct gaaggtcgtt gtagaggcag tcggccagca
     4441 tgtgcggatc gccggctctc agcgcctgca gcacgttcgc ctccacggtc ggtgccaccg
     4501 gggcagggaa gatgtcctgg gagttgcggt cgcggtggcg gtcgagctca ctgtagacct
     4561 cgggtgtgga cagcccgaag tcggcgagcg cgagcaccca ctggaattgg cccttcgcca
     4621 gcgctgggct caactggtca ccgcgacccg tgccgatggc ggtgccgccg gtgaacgcga
     4681 acggcacgtc ggccccgagt tcggctgcga gatgcagcat ctcctcgcgc ggaagcgctg
     4741 tgccccacag ggtgtcgcac gccaggaggg tggccgcggc atccgccgac ccgcccccca
     4801 tgccgccggt gaccggcacg tgcttgtcga tctcgaggcg caccccgccc cgatacccgg
     4861 tgcgccgcgc aagcaggcgt gcggctttga tcgcgatgtt gcgggcatcg gtgggcaccc
     4921 gcgagagttc gacgctgccg gtcacgctga cggagaagtc gtcggccggg taggcgcgaa
     4981 cgtcctcgta gagggagacc gcctggtagg cgatcgcgac gtcgtgatag ccgtcctcga
     5041 gcagagcccc gaccttgagg aagacgttga tcttgcccgg tgcccgagtg tgcaccatgc
     5101 cggtcgtggc ctcggaggtc atgagacaaa cctagcctga gcgcggttga ggtctcgccg
     5161 ctcagcccgc gcgggcgacg ctcaggaagt cgtccacggt cagctcctcg ccgcgggcgg
     5221 tcggcgcgat gcccgccgcc tcgagccggg ccgacgcggc ggc
//
LOCUS       DAOSOU010000156         5252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_15842_length_5252_cov_5.306715, whole genome shotgun sequence.
ACCESSION   DAOSOU010000156 DAOSOU010000000
VERSION     DAOSOU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15842_length_5252_cov_5.306715"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLW15_08655"
     CDS             complement(<1..751)
                     /locus_tag="PLW15_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT34502.1"
                     /translation="MAFDIDNYTATSTSVMWGDLDFGDFETNPLPESTLRSLRYMCDI
                     EYHTVCYLRDLLVTPSHKEADVSAFMTMWNREEFWHGEALAAVLGAHGITVDFDELKA
                     TRLKLGWKDRLDPIKQSLLGNIVGKDFIAVHMIWGAANEWSAVAAYNRLAELEQHPVL
                     AVLLKRIAKQEARHVAFYATQARERLAKSKKAQVLARFALKNAWGPVGSSVMEPAEVT
                     HVMGHIFAGEEGLREIRKLDEHISRMPGLEGL"
     gene            complement(741..3095)
                     /locus_tag="PLW15_08660"
     CDS             complement(741..3095)
                     /locus_tag="PLW15_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT34503.1"
                     /translation="MTPTRTTTTRTKSSPVLTNEHVFLTGATGFVGQAVLERLLSSHP
                     DTRISILVRGKGSQTGEGRLANLMRKPVFANWIESIGEDAAAKAIAKRITVIEGSLTN
                     VGTLPSDIDVVIHGASTVSFDPPIDEAFDTNVGGAHGVYGALLASGSDPHVVHVSTAY
                     VGGIRKGIAPEASLTHTVDWRAEYEAAKSARTRVELESRQPEALRQHLAKARARHGKT
                     GPQAVAGFAEAARSEWVRERLVDYGRTRAESLGWTDVYTLTKAFAERAAEELWSQAGH
                     RLSVVRPSIIESALHHPFPGWIDGFKVADPLIIAYGRGQLPDFPGLPDSVLDVIPVDF
                     VVNAALAVAARPAKPNEPEYFQVVSGKSNPLPFHRMYENVNQYFTANPLPDEKGDIKV
                     PLWRFPGGQKVEKALAKREHQVVRAERIIERLPTTPRTRKWLDQVSRGKHGLSQLRAF
                     TELYRAYVQTEIIFDDTNTRALLAGLPKKVQETEGFDVTEIDWEHYFQEIHFPAVTTL
                     TRAFATRPSAKTRGARVLPVRSDVVAIFDLEGTVLESNLVKQYLLLWAGSVPRSRVAH
                     DLANFTFSLPKYLRAERRDRGEFIRTFMRRYKGFKVSEIERLVRGSFGRAMMRRALPD
                     ALRRVQEHRDAGHRTILVTGTIDLMVAPFVPYFDEVVAGRMQEKDGVLTGFLADPPLV
                     DEARAAWLRHYADQNGFNLSQSYGYGDSHADLVWLQLLGNPSAVNPDIQLYRHAQEKR
                     WNVLDWKRHPAAVRQVQRLESEKQTPTEGGGTSLEESPAASHGI"
     gene            complement(3092..3898)
                     /locus_tag="PLW15_08665"
     CDS             complement(3092..3898)
                     /locus_tag="PLW15_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34504.1"
                     /translation="MPEHQETSVGRADFPAPDFDVRDFARTAAGSHRESMDLTAYRSA
                     PLSDATLRIVRYLQIVERATMTHLRSVLVTATHKDARITAFLTTWAFEKYWIADALEQ
                     VLLAHQPEGAEPDRSPFRTPVERTIRESIVANIIGVPMIAVHMTLGTVDEWLTQAAYR
                     RLGALDPNPEFDRTVTTFLEIKQRQLSFFEAQSRFRLTESPQARKLTRKRLTKTPWPI
                     GAKAEPAHETHFFFDHLFVTAPALVAELDERIDTLPGQSGLALIRKAARL"
     gene            3982..4809
                     /locus_tag="PLW15_08670"
     CDS             3982..4809
                     /locus_tag="PLW15_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT34505.1"
                     /translation="MATALVTGGTSGIGAEFARQLAASGYDLVLVARNPDRLASAAER
                     LHADAGINVETISADLSDRADVARVAARIEDAEHPIDLLVNNAGEGFRTALASADVAA
                     HEHAFDVMCRAVLVLGGAAARAMSGRRGTIINISSLQTYLATGAYAANKAWVTAYSQS
                     LAVELRGTAIGVTVVLPGWVRTEWHERAGVRRSSLPGFLWTQPEAVVRIALRDARRGK
                     VVCIPTVRYRVLGWFARHLPNAAVRRISAGISSGRDEASPSDSAGGAESSAMKADTA"
     gene            4806..>5252
                     /locus_tag="PLW15_08675"
     CDS             4806..>5252
                     /locus_tag="PLW15_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT34506.1"
                     /translation="MSKGEKRDRFNSPAVATVRFVAQRGLLKPLVWSLVRVTVYGKDQ
                     IADLDRPFIAVANHSSHLDAPLVIGALPRPLARYLAAGAAADYFFDVQWRKWLTTLFF
                     NAFAIERNSEGKRSGSSRSLLERRVPLLIFPEGGRSKTGVMGHFKHG"
BASE COUNT          805 a         1663 c         1863 g          921 t
ORIGIN      
        1 tgagaccctc gagcccgggc atccgggaga tgtgttcgtc gagcttgcgg atctcccgca
       61 gtccctcctc gcccgcgaag atgtgtccca tcacatgggt gacctcggcg ggctccatca
      121 cgctcgagcc gaccggaccc caggcgttct tcagcgcgaa gcgcgcgagc acctgcgcct
      181 tcttgctctt cgcgaggcgc tcgcgggcct gggtggcgta gaaggccacg tggcgggcct
      241 cctgcttggc gatgcgcttg agtagcacgg cgagcacggg gtgctgctcg agctcggcaa
      301 ggcgattgta ggcggcgacg gccgaccact cgttcgcggc gccccagatc atatgtacgg
      361 cgatgaagtc cttgccgacg atgttgccca aaagtgactg cttgatcggg tcgagccgat
      421 ccttccagcc gagcttgagg cgcgtcgcct tgagctcgtc gaaatcgacg gtgatgccgt
      481 gcgcgccgag cacggcggcg agggcctcgc cgtgccagaa ctcctcgcgg ttccacatgg
      541 tcatgaaggc cgagacatcc gcctccttgt gcgagggcgt caccagaagg tcgcgtaggt
      601 agcagaccgt gtggtactcg atgtcgcaca tgtaacggag gctgcgcagc gtggactcgg
      661 gcagcgggtt ggtctcgaag tcgccgaagt cgaggtcacc ccacatcacg ctcgtcgacg
      721 tggcggtgta gttgtcgata tcaaatgcca tgggatgccg cggggctctc ctcgagagag
      781 gtcccgcctc cttccgtggg ggtctgtttc tcggattcta ggcgctgaac ctgtcgaact
      841 gccgccgggt ggcgcttcca gtccagaaca ttccagcgct tctcctgcgc gtgccggtag
      901 agctggatgt cggggttgac cgcgctgggg ttgccgagca gttggagcca gaccaggtcg
      961 gcgtggctgt cgccgtagcc gtacgactgg ctgaggttga atccgttctg gtcggcgtag
     1021 tggcgcagcc acgcggcgcg ggcttcgtcg acgagcggcg ggtcggccag gaagccggtg
     1081 agcacgccat ccttctcctg catgcggccc gcgacgacct cgtcgaagta ggggacgaac
     1141 ggggcgacca tgaggtcgat cgtgccggtg acgaggatgg tgcggtgacc ggcatcccgg
     1201 tgctcctgta cacgtcgcag ggcgtcgggc agggcgcggc gcatcatcgc ccgaccgaag
     1261 ctgccgcgca ccaggcgttc gatctcgctg accttgaacc ccttgtagcg acgcatgaag
     1321 gtgcggatga actcgccgcg gtcgcggcgt tcggcccgga ggtacttggg cagcgagaag
     1381 gtgaagttcg cgagatcgtg cgcgacccgc gaccggggca cgcttcccgc ccacagcagc
     1441 aggtactgct tgaccaagtt ggattcgagc accgtgccct cgaggtcgaa gatggcaacc
     1501 acatcggagc gcaccgggag cacccgtgcg ccacgcgtct tggcggaggg ccgggtggcg
     1561 aacgcgcggg tcagcgttgt cacggccggg aagtggatct cctggaagta gtgctcccaa
     1621 tcgatctcgg tgacgtcgaa gccctcggtc tcctgcacct tcttcggaag gccggccagc
     1681 agggcgcggg tattggtgtc gtcgaagatg atctccgtct gcacgtacgc gcgatacagc
     1741 tcggtgaacg cgcgcagctg gctcagcccg tgcttgcccc gggagacctg gtcgagccac
     1801 ttgcgggtgc gcggggtcgt cggcaggcgc tcgatgatcc gctcggcccg caccacctgg
     1861 tgttcgcgct tggcgagagc cttctccacc ttctggccgc ccgggaagcg ccacagcgga
     1921 accttgatgt cgcccttctc gtcgggcagc gggttggcgg tgaagtactg gttgacgttc
     1981 tcgtacatcc gatggaaggg caggggattg ctcttgcccg acacgacctg gaagtactcg
     2041 ggctcgttcg gcttggccgg ccgcgcggcg accgcgaggg ccgcgttcac cacgaagtcg
     2101 accgggatga cgtcgagcac ggagtcgggc aggccgggga agtccggcag ctggccgcgc
     2161 ccgtaggcga tgatcagcgg gtcggcgacc ttgaagccgt cgatccaacc ggggaagggg
     2221 tggtgcagcg cgctctcgat gatcgacgga cgaaccaccg acaggcggtg gcccgcctgc
     2281 gaccacagct cttcggcggc ccgctcggcg aacgccttgg tgagcgtgta gacgtcggtc
     2341 cagccgaggc tctcggcgcg ggtgcggccg tagtcgacga gtcgctcgcg cacccactcg
     2401 gagcgggcgg cctcggcgaa acccgccacg gcctgcggtc cggtcttgcc gtgacgggcg
     2461 cgcgccttcg cgaggtgctg gcgcagggcc tccggttggc gcgactcgag ctcgacgcgg
     2521 gtgcgggccg acttcgcggc ctcgtactcc gcgcgccagt ccacggtatg ggtgagggat
     2581 gcctcgggcg cgatgccctt gcggatgccg cccacgtacg cggtcgagac gtgaaccacg
     2641 tgcgggtcgc tgccgctggc cagcagggca ccgtagacgc cgtgcgctcc gccgacgttg
     2701 gtgtcgaagg cctcatcgat gggcgggtcg aacgacaccg tcgatgctcc gtggatgacc
     2761 acgtcgatat ccgaggggag agtgccgacg ttggtcaggc tgccctcgat cacggtgatg
     2821 cgcttcgcta tggccttcgc cgcggcatcc tcaccgatcg attcgatcca gttggcgaag
     2881 acgggcttgc gcatgaggtt ggcgagccga ccttcgcccg tctggctgcc cttgccgcgc
     2941 acgaggatcg agatgcgggt gtccggatgc gacgacagca gtcgctcgag caccgcctgt
     3001 cctacgaacc cggtcgcgcc cgtgaggaac acatgctcgt tggtgagcac cggtgaggac
     3061 ttcgtgcggg tggtcgtcgt gcgtgtggga gtcacagccg tgcggccttt cggatcaggg
     3121 cgagtccgga ctggccgggc agggtgtcga ttcgctcatc gagctcggcc acgagggcgg
     3181 gtgcggtgac gaacaggtgg tcgaagaaga aatgagtctc gtgggcaggc tccgccttgg
     3241 cgccgatggg ccacggggtc ttcgtcagtc gcttgcgggt gagcttgcgg gcctgcggcg
     3301 actcggtgag ccggaagcgg gactgggcct cgaagaagct cagctgacgc tgcttgatct
     3361 cgaggaaggt cgtgacggtg cgatcgaact ccgggttcgg gtcgagagcc ccgaggcgac
     3421 ggtacgcggc ctgggtgagc cactcgtcga ctgttccgag ggtcatgtgc acggcgatca
     3481 tgggaacgcc gatgatgttg gcgacgatgg actcgcggat ggtgcgttcg accggcgtgc
     3541 ggaaggggga gcggtccggc tcggcaccct cgggctggtg agcgagcagc acctgctcga
     3601 gcgcgtcggc gatccagtac ttctcgaacg cccaggtcgt gaggaacgcg gtgatgcgcg
     3661 catccttatg cgtcgcggtc acgagcacac tgcgcaggtg cgtcatcgtc gcgcgctcca
     3721 cgatctggag gtaccggacg atccgtagcg tggcgtcgct gagcggcgcg gaacgatagg
     3781 cggtgaggtc catcgactcg cggtggctcc cggccgccgt gcgcgcgaag tcccgaacat
     3841 cgaagtcggg tgcggggaag tcagcgcggc ccacagacgt ctcctggtgc tcgggcatca
     3901 gtactacctc cgggaaacgg gacaagttcg gcccagtcta cccgcccatc gctacggtgc
     3961 ccgtagcgta atgtatccac catggcaacg gctctcgtca cgggcggcac atccggcatc
     4021 ggtgccgaat tcgcccgcca gctggctgcc tccggatacg acctcgtgct cgtcgcacgc
     4081 aaccctgacc gcctggcgtc cgcggcggag cgactgcatg ccgatgcggg catcaatgtc
     4141 gagacgatca gcgccgacct cagcgatcga gcggatgtcg cgcgagttgc cgcgcgaatc
     4201 gaggatgccg agcatccgat cgaccttctc gtgaacaacg cgggtgaggg attccgcacc
     4261 gctctcgcat cggctgatgt agccgcgcac gaacatgcct tcgacgtcat gtgtcgagcc
     4321 gtgctcgtcc tggggggtgc ggccgcacgt gccatgagcg gtcgtcgcgg cacgatcatc
     4381 aacatctcca gcctgcagac gtatctggcc accggggcat atgccgcgaa caaggcgtgg
     4441 gtcaccgcat acagccagag tctcgcggtg gagcttcgtg gcacagcgat cggcgtcacg
     4501 gtggtgcttc ccggttgggt gcgcaccgag tggcacgagc gggcgggcgt gcggcgcagc
     4561 tcgctgccgg gctttctctg gacgcaaccc gaggctgtcg tgcgcatcgc gctgcgggac
     4621 gcgcggcgcg gcaaggtcgt gtgcataccc actgttcggt accgagtgct cggctggttt
     4681 gcccgccacc tgccgaacgc ggccgtgcgc cggatttcag cgggcatctc atcgggccgc
     4741 gacgaggcgt caccaagcga ttcggcgggc ggcgccgaat cttcagccat gaaggcggac
     4801 accgcatgag caagggcgag aagagggatc ggttcaattc tccggctgtc gcaacggtgc
     4861 gattcgtcgc acagcgcggc ctgttgaaac cgctggtctg gtcgttggtg cgcgtcaccg
     4921 tctatggcaa ggaccagatc gctgacctcg accgtccctt catcgccgtg gccaatcact
     4981 ccagccatct ggatgcgccc ctcgtgatcg gcgcgctgcc ccggcctctg gcgcgctacc
     5041 ttgccgcggg tgccgccgcg gactacttct tcgacgtgca gtggcgcaag tggctgacca
     5101 cgctgttctt caacgcgttc gccatcgagc gcaattccga gggcaagcgc agcggttcgt
     5161 cccgttctct gctcgagcga cgagtgcccc tgctgatctt ccccgagggt ggccgctcca
     5221 agacgggcgt catgggccac ttcaagcacg gt
//
LOCUS       DAOSOU010000157         5199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_16066_length_5199_cov_5.958981, whole genome shotgun sequence.
ACCESSION   DAOSOU010000157 DAOSOU010000000
VERSION     DAOSOU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16066_length_5199_cov_5.958981"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(517..1107)
                     /locus_tag="PLW15_08680"
     CDS             complement(517..1107)
                     /locus_tag="PLW15_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HOT34507.1"
                     /translation="MPLWAQVVAWVALLAIVGALGSSGKNDSKASGEPIVVDSGSASK
                     STTSPAADPTSTVVATTATTEAATTTSAPPPPTTPPTPAPTSQAPARPTMTPSQQAAV
                     RSAESYLEFKGFSRQGLIDQLSSEYGDQFSVADATFAVESLTVDWNEQAARSAQSYLE
                     FKGFSCQGLIDQLSSEYGDQFTAEQARFGAAQVGLC"
     gene            complement(1866..1928)
                     /locus_tag="PLW15_08685"
                     /pseudo
     CDS             complement(1866..1928)
                     /locus_tag="PLW15_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2318..2446)
                     /locus_tag="PLW15_08690"
     CDS             complement(2318..2446)
                     /locus_tag="PLW15_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34508.1"
                     /translation="MAVGEFGATVFLSRGALSCVLGLIAALSVALVDRATDDGPAS"
     gene            2624..2962
                     /locus_tag="PLW15_08695"
     CDS             2624..2962
                     /locus_tag="PLW15_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT34509.1"
                     /translation="MVQESKRLWLHGLLEVCVLGLLEERRDYGLALANRLEDAGLGSV
                     PGGTMYPALLRLETAGLVRSVREPSTTGPPRKYFEVTVEGRSVLAQRRHEWREFSRSI
                     DAVLGLEAHT"
     gene            2959..3774
                     /locus_tag="PLW15_08700"
     CDS             2959..3774
                     /locus_tag="PLW15_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT34510.1"
                     /translation="MTGWRNNFLRALAERAVSAEMGDELLREADLEAAERGLPPEQIF
                     GPATAYADQLARALRVPSGSPLALAEQSSPALRLNGISKRYGRRQVLNDVTLEVRHGE
                     SVAVVGANGSGKSTLLKVCAGTVKPDAGTVERTEHVGYVPQSGGTAEFLTATEHFELF
                     AAARQNSRRRGRATGERLAASLGWSPSSEHTAGQLSGGIRQKLNVVLGELSGPDLLLL
                     DEPYQGFDHGSYIDLWTQIDRWREKGTGVLIVTHMLGDLDRVDRIIELDSPED"
     gene            3777..4469
                     /locus_tag="PLW15_08705"
     CDS             3777..4469
                     /locus_tag="PLW15_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34511.1"
                     /translation="MQMIAIARATSRGLVRRRSALLLLTVLPLAFYLARRDVTHSAIV
                     VLALGLGWAAAMLSLFSTVNALELDHRLRVAGFSARSILLGRILAISSMGLALMVGYL
                     VLAVVDQDVTRLGGVALMMLCALLVGVPLGITVGLVLKRELEGALALIILLATQFMTD
                     PERPVAHWLPLWSVRGVADWALDLKDRTFLVDGLAHAGATLALLSLVIAGFSALRLRI
                     HRYRPLTSSWSD"
     gene            complement(4643..4810)
                     /locus_tag="PLW15_08710"
     CDS             complement(4643..4810)
                     /locus_tag="PLW15_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34512.1"
                     /translation="MRTSRMLLIGLIAVLPITSAACTYDEQLTFFSYLGCVVVPVFTG
                     QPCTGPTLPGL"
     gene            complement(4984..>5199)
                     /locus_tag="PLW15_08715"
     CDS             complement(4984..>5199)
                     /locus_tag="PLW15_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT34513.1"
                     /translation="FAFAMAVGEFGATVFLSRAARPTVPTLIGLLLGRPGTANLGQAL
                     ALSCVLGLIAALSVALVDRATDDGPAF"
BASE COUNT          868 a         1758 c         1695 g          878 t
ORIGIN      
        1 ctccggcggt atccgccgtc gtcgatgagc gcgttgacga acgaggccgc ctcccggaga
       61 tgggcaggcg cacccgcgac ccggcgcatc gcccggcggg agcgacgctg gccgaccggc
      121 agctccgact ccccgatcga gagcgcagcc cgaccgatga gatgcggcac gatgaacatg
      181 ccgtgagcct aggaggctgg cgctcgagag aagcgacccg cggtccacgt gctgttcttg
      241 caggctgaac gccggtcggg tcggcacggg gaaggattcg agaaatggga tccaggcccg
      301 aacggtcggc gagtcgagcc aggtgctcgc gtcggtcacg cccgacgtgg tcagttcgac
      361 ccacccggcc acaacgcact cgtctacgac ctgcttccac aactcctgct gcgcattctg
      421 atcccggttg gccgacccgc cggggatgtc gaccccggtc agggccacgt cgacacggag
      481 gctcagggca ccgaaggccg tcgccttctc gtcggcctaa cagaggccga cttgcgctgc
      541 tccgaaccgg gcctgttccg ccgtgaactg gtccccgtac tccgatgaca gttggtcgat
      601 gagcccttgg cacgagaagc ctttgaactc gaggtaggac tgcgctgatc gagccgcctg
      661 ctcgttccag tcaacggtca aactctcgac ggcaaacgtt gcatcagcca ccgagaactg
      721 gtcgccatac tctgacgaaa gttggtcgat caggccctgc cgcgagaacc ccttgaactc
      781 gagatacgac tcagcggagc gtacggccgc ctgttgagat ggcgtcatcg tcggacgagc
      841 aggcgcttgc gatgtcggcg caggagtcgg tggagtcgtg gggggcgggg gcgctgacgt
      901 cgtggtcgct gcctccgttg tcgcggtcgt cgcgacaacg gtgctcgtcg gatccgccgc
      961 tgggcttgtc gtggacttgc tggcggatcc actgtcgacc acgatcggct cgcccgatgc
     1021 tttcgagtcg ttcttgccag acgaaccgag cgcgccgacg atggcaagta gggcgaccca
     1081 ggcgacgacc tgggcccaca gcggcaacgc ccgaaacttg cgccatgttc gcgctagtgg
     1141 gctggcggca gagcgttggt cgctctcgcc cggagcgacg ggtgcaacat atgcgggtgg
     1201 accctggggg gctgcgatcg gtgcatctgt cgcctgtacg ccgccactac tgacggcgct
     1261 ggtccactgc tgtccatccc aataccggta ctcatttcga ccgtacggat ctgggtgcca
     1321 gcctgccggt cccgtgctca ctcaaaccgc cctcctcggt gaatgaatga acctacggca
     1381 acgttggcga cggcgcaacg acttcccaga ccgcctgcgg ctcctcgttg ccgtctgcac
     1441 cgccgggtga tcggctgcca tggcgaattc ccgattggcc tgactccatt ctcagttcca
     1501 aagccaggag caacttgccc cgggtttcgt gaagtcagtc cacccgaatc tcacacatcg
     1561 gtaggtcacg tctccggttc ggcaatccgg tctccatgcg cccctcccag acgactcgag
     1621 gacggcgttg tcgaaacagt cgccgatcaa tcccatcgac gcgacgagcg ccgaccactg
     1681 ggccggacgg gtgaaggccc gagaagtgaa ctgcgtcttg cggggcgact cgatgagcca
     1741 ttccgtcacg accaggcgtc gcccatcacg tccggatcgg cgaggtcggt gaactgctcc
     1801 gcgagcggtc ccaggtcggc tgagctgacg gtcacggctg gtcgactctc actctcgagt
     1861 gcacgccgca gattttcgtt ccgggagata ccgagccgcc gggcgcgttc gtcgatcgct
     1921 gccaccacgt catcgggaac acctcggatc gacacatcgg ccactcggca cctccgtgat
     1981 ggttcaggat attgcgaggc gccggcgtgc gggtcaggtc agccgaagac gcttgtaacg
     2041 gcccgccgct cgactgctga tcggttgttc cttccgatag tcccgggacg acaccgggtc
     2101 gagctccgct cgactgagct tacgagcgag gtctgtcacc cagacttccg cggacatccc
     2161 acggtgcgct gccaatgcat tcccgctgaa gcatgctcac caccctccgt gaacaggcga
     2221 cccagtccat cctgccgatg gctgctccgg cccgagcgca acgccaccct cggtgggatc
     2281 cacgcgcccc ggacgcgtgg atcccacgga gcgtgagtca agatgccggg ccgtcgtcgg
     2341 tcgcccgatc gacgagcgcc acggagagcg ctgcgatcag cccgagcaca cagctgaggg
     2401 cgccacgact caggaagacg gtcgcgccga actcccccac tgccatggcg aacgcgaagc
     2461 ccgatgccac gccgaccggt cgtctcagcg cgggccacac gcccgacggc gcaacgcatc
     2521 ggtcgatcga ctggcagagg tcgtgcatct ggctgctccc gccgctgctc cgatcaagcg
     2581 tcgccagttg acagatggtc tggttaccag agagtcttac tagatggttc aggaatcgaa
     2641 acgactgtgg ctgcacggcc tgctcgaggt gtgcgtgctc ggactgctcg aggagcggcg
     2701 cgactatggg ctcgccctgg cgaaccgcct cgaggacgca ggactcggct cggtgcccgg
     2761 cggcaccatg tatccagcgc tcctccgctt ggagaccgcc gggttggtgc gctctgtccg
     2821 tgagccgtcc acgacaggtc cgccccggaa gtacttcgag gtgaccgtcg agggccgttc
     2881 cgtgcttgcc cagcgacgcc acgagtggcg ggagttcagc cgaagcatcg acgccgttct
     2941 cgggctcgag gcgcacacat gaccggatgg cgaaacaact tcctccgcgc cctggccgaa
     3001 cgagcagtgt cggccgagat gggagacgag ttgctgaggg aggccgacct ggaggcggct
     3061 gaacgtggac tccctccgga acagatcttc ggaccagcta ccgcgtatgc cgaccagctc
     3121 gcccgagccc tccgcgtccc gtcgggctca ccgctcgctc tcgccgagca gagctcgccg
     3181 gcgctccgac tcaatgggat ctccaagcgt tacgggcgac gtcaggttct gaacgacgtc
     3241 acgctcgagg tgcggcacgg cgaatcggtg gcggtcgtcg gcgccaacgg atccgggaag
     3301 tcgacgttgc tgaaggtctg tgcgggaacc gtcaagcccg atgccggcac cgtcgagcgc
     3361 accgaacacg tcggatacgt cccgcaatcg ggcgggaccg cagagttcct gactgcgacc
     3421 gaacacttcg agctcttcgc agcggcgcgt cagaactcac gacggcgtgg gagagcgacc
     3481 ggcgaacgac tcgcagcttc gctggggtgg tccccgtcgt cggagcacac cgccggccag
     3541 ctcagtgggg gcattcgcca gaagctgaac gtcgtgctcg gggagctgag cggaccggac
     3601 ctgctgctcc tggacgagcc gtaccaggga ttcgatcacg ggtcctacat cgatctgtgg
     3661 acgcagatcg acagatggcg agagaaaggc actggtgttc tgatcgtgac ccacatgctc
     3721 ggcgacctgg accgggtcga ccgaatcatc gagctcgact caccggagga ttgatgatgc
     3781 agatgatcgc catcgccaga gcaacgtcgc gggggcttgt tcgtcgacgc agcgcactgc
     3841 tccttctcac cgtcttgccg ctcgccttct acctggcccg acgcgacgtg acccattcgg
     3901 ccatcgtggt cttggccctc ggcctcggct gggccgccgc gatgctctcg ttgttcagca
     3961 ccgtcaatgc actcgagctc gatcatcggt tgcgagtcgc cggcttcagc gcccggtcga
     4021 tccttctcgg ccggatactg gccatctctt ccatgggatt ggccctgatg gtggggtatc
     4081 tcgtgcttgc cgtcgtcgat caagacgtga ccaggctcgg cggcgtcgcg ctcatgatgc
     4141 tgtgtgcact gctcgtcggg gtaccactcg gaatcacggt ggggctcgta ctcaaacgcg
     4201 aactcgaagg cgctttggcc ctcatcatcc tgctggcaac ccagttcatg accgatcccg
     4261 aacgccccgt cgcgcactgg ctccccctct ggtccgtgcg aggtgtggcg gactgggctc
     4321 tcgacctcaa ggaccgcacg ttcctcgtcg acgggctcgc acatgctggg gccaccttgg
     4381 ccctcctgtc gttggtcatt gccggtttct ccgcgctccg actcagaatt caccggtacc
     4441 ggccgctgac cagtagctgg tcggactgaa ggcggagcgc gacgagcgcg gcgtcagccc
     4501 gaccgagcga agactcggac gcttgcggct tcgtctgcga ggccggcccg tggggccgat
     4561 cgacgcgggt gccggcgctc gctccgcgcc cggccaacgg tccgccgtcc gtgggtctga
     4621 cgacgcgccg taccgactcc gcctagagac ccgggagggt cggcccggtg cacggctgcc
     4681 cggtgaagac cggcaccacc acacagccga ggtacgaaaa gaacgtcagc tgctcgtcgt
     4741 aggtgcaggc cgcagacgtg atcggcagca cggcgatcag tccgatcaga agcatccggc
     4801 tcgttcgcat gtggcaaccc cctgttgcag ccgcccccga cgggtggcgt cggtcgagta
     4861 tcgcacgaac cgtggtcagg gcgcctcggt tcggtcccgt ctcgccgccc cgagaagtcg
     4921 gagattcgag cacccgcaca tccgtcctcg cgtcggtccc cggcgtgaac ctccactcgg
     4981 gattcagaag gccgggccgt cgtcggtcgc tcgatcgacg agcgccacgg agagcgctgc
     5041 gatcaggccg agcacacagc tcagggcgag ggcttggccg aggttcgcgg tccccggccg
     5101 gccgagaagc aggccgatga gcgtcggcac ggtcgggcgc gcggcacgac tcaggaagac
     5161 ggtcgcgccg aactccccca ctgccatggc gaacgcgaa
//
LOCUS       DAOSOU010000158         5174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_16176_length_5174_cov_6.730221, whole genome shotgun sequence.
ACCESSION   DAOSOU010000158 DAOSOU010000000
VERSION     DAOSOU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16176_length_5174_cov_6.730221"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..2022
                     /gene="ilvD"
                     /locus_tag="PLW15_08720"
     CDS             313..2022
                     /gene="ilvD"
                     /locus_tag="PLW15_08720"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOT34514.1"
                     /translation="MPETPATPVDIKPRSRTVTDGIEATTSRGMLRAVGMGDADWDKP
                     QIGIASSWNEITPCNLSLDRLAQAAKEGVHGGGGYPLQFGTVSVSDGISMGHEGMHFS
                     LVSREVIADSVEVVMQAERLDGSVLLAGCDKSIPGMLMAAARLDLASVFLYAGSIAPG
                     WVKLSDGTEKDITIIDSFEAVGAVKAGKMSAEDAKRIECAFAPGEGACGGMYTANTMA
                     SVAEALGMSLPGSASPAAADRRRDYYAHRSGEAVVGMLERGHTARDILTLKAFENAIA
                     VVMALGGSTNAVLHLLAIANEADVPLTLDDFNRIGETIPHIGDLKPFGKYVMNDVDRH
                     GGIPVLMKALLDAGLMHGDVLTVTGKTMAENLAELDPEALDGEVLRTLDNPIHPTGGL
                     TILHGSLAPEGAVVKTAGFDATVFEGPARVFERERAAMDALTNGEIKAGDIVVIRYEG
                     PKGGPGMREMLAITAAIKGAGLGKDVLLLTDGRFSGGTTGLCIGHIAPEAVDAGPIAF
                     VRDGDLIRVDIAARSLDLLVEPAELEARRNGWAPLPPRYTRGVLAKYTKLVHSASVGA
                     VTG"
     gene            2083..3867
                     /locus_tag="PLW15_08725"
     CDS             2083..3867
                     /locus_tag="PLW15_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOT34515.1"
                     /translation="MSTDTSPRPKSTAPEILTGSGAILRSLEHLGISDVFGLPGGAIM
                     PFYDELMASTSIRHILVRHEQGAGHAAEGYASSTGKIGVAIATSGPGATNLVTAIADA
                     YMDSVPLLAITGQVFSTSMGTDAFQEVDIVGITMPITKHSFLVTRAEDVPATLAAAYH
                     IATTGRPGPVLVDVTKDAQQKSAPFIWPPKVDLPGYRPVMKAHGKQVQAAAQLLVESE
                     RPVFYVGGGVIRSGASKELLALATAVGAPIVTTLMARGAFPDSNPLNLGMPGMHGTVP
                     AVLGLQESDLIISLGARFDDRVTGKASEFAPNAKIVHVDIDPAEIGKIRAADVPIVGD
                     AKDVIVDLTAAFAEASASQKPDYTAWWTRLNELKKNYPLGYTMPDDGLLSPQKIIERI
                     GQLSGPEAIYAAGVGQHQMWAAQFIKYERPNAWLNSGGAGTMGYGVPAAMGAKVANPD
                     RVVWAIDGDGCFQMTNQELATCTINNIPIKVAIINNSSLGMVRQWQTLFYDGRHSFTD
                     LNTGHDTVMIPDFLKLAEAYGCLAIRVRTEDEVDAAIRLALETNDRPVVIDFIVSRDA
                     MVWPMVPQGVGNSSVQYARDHAPEWEEE"
     gene            3870..4376
                     /gene="ilvN"
                     /locus_tag="PLW15_08730"
     CDS             3870..4376
                     /gene="ilvN"
                     /locus_tag="PLW15_08730"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOT34516.1"
                     /translation="MSHVLSLLVEDKPGLLTRVAGLFARRGFNIESLAVGKSEIEGLS
                     RITVVVDVEQLPLEQVTKQLNKLVNVIKIVELDPTQSVTREHLLIKVRVDNTTRSQIL
                     EAVTLFRAKVVDVATDALVIETTGDTPKIQAFLRVLEPYGIKEIAQSGLLAIGRGSKS
                     ITERVFKN"
     gene            4459..>5174
                     /gene="ilvC"
                     /locus_tag="PLW15_08735"
     CDS             4459..>5174
                     /gene="ilvC"
                     /locus_tag="PLW15_08735"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOT34517.1"
                     /translation="MAAELLYDNDADISLIQGKKVAIIGYGSQGHAHAQNLRDSGVEV
                     KIGLKDGSKSAPKATEEGFEVLSVADAAEWADVIMILAPDQHQRAIYSGSIKDKLKPG
                     KTLAFAHGFNIRFGYIDAPEGVDVILIAPKAPGHTVRREFVAGRGIPDIIAVERDASG
                     HAWDLALSYAKAIGGTRAGVIKTTFTEETETDLFGEQAVLCGGTSQLVMYGFETLIEA
                     GYQPEIAYFEVLHELKLIVDL"
BASE COUNT          943 a         1823 c         1624 g          784 t
ORIGIN      
        1 accggtgcga ccctcgccaa cttccgggtg ccgggaccca cccaggtcgc tcgcgctctc
       61 gccctgctcg cggacttccg cgagggcgac gtgcacgagc aatagcgcgc tggagcggta
      121 gcgcgtgggt tgccgtgctc gaacgaccga cctctcccgt gcccttctgt tatgcaggac
      181 cgtcgagaga tggtcagctc tcctgcacaa cagaaggaag tgcgagggat cagtcgaatc
      241 ggcgatgccg cgtgccgggc tcggggcccc accccgggca tccgcacccc cgcaaagcag
      301 taccctcgta gcatgccgga aacgcccgca actcccgtag acatcaagcc ccgctcccgc
      361 accgtcaccg acggaatcga agccaccacg tcgcgcggaa tgctccgcgc tgtcggcatg
      421 ggtgacgccg actgggacaa gccgcagatc ggcattgcga gttcctggaa cgagatcacc
      481 ccctgcaacc tgtcgctcga ccgcctcgct caggcggcga aggagggcgt gcacggcggc
      541 ggcggttacc cgctgcagtt cggcaccgtc tccgtctccg acggcatctc gatgggtcac
      601 gagggcatgc atttctcgct ggtctcgcgc gaggtcatcg ccgactcggt cgaggtcgtc
      661 atgcaggccg agcgcctcga cggctcggtg ctgctggccg gatgcgacaa gtcgatcccc
      721 ggcatgctca tggcggccgc gcggctcgac ctcgcgagcg tgttcctcta cgccggatcg
      781 atcgcccccg gctgggtcaa gctcagcgac ggcaccgaga aggacatcac gatcatcgac
      841 tccttcgagg cggtcggcgc ggtcaaggcc ggcaagatga gcgccgagga cgccaagcgc
      901 atcgagtgcg ccttcgcccc cggggagggt gcctgcggcg gcatgtacac ggccaacacg
      961 atggccagcg tcgccgaggc gctcggtatg agcctgcccg gctccgcgag cccggcggcg
     1021 gccgatcgac gccgcgacta ctacgcgcac cggtcggggg aggccgtggt gggaatgctg
     1081 gagcgcgggc acacggctcg cgacatcctc acgctcaagg cgttcgagaa cgcgatcgcc
     1141 gtcgtcatgg cactgggagg ctcgaccaac gccgtgctgc acctgctggc catcgccaac
     1201 gaggccgacg tgccgctcac gctcgatgac ttcaaccgca tcggcgagac gattccgcac
     1261 atcggtgacc tcaagccgtt cggcaagtac gtgatgaacg acgtcgatcg gcacggcggc
     1321 atcccggtgc tcatgaaggc gcttctggat gccggactca tgcacggcga cgtgctcacg
     1381 gtcaccggca agacgatggc cgagaacctg gccgagctcg accccgaagc tctcgacggc
     1441 gaggtgctgc gcacgctcga caaccccatc caccccaccg gtggactcac catcctgcac
     1501 ggctcgctgg cgcccgaggg tgccgtcgtg aagacggccg gcttcgacgc gacggtcttc
     1561 gagggcccgg cccgcgtgtt cgagcgcgag cgggccgcca tggatgccct caccaacggc
     1621 gagatcaagg ccggggacat cgtcgtcatc cgctacgagg ggccgaaggg cggacccggc
     1681 atgcgcgaga tgctcgcaat caccgccgcc atcaagggcg ctggcctcgg aaaagatgta
     1741 ctactcttga cggacggcag attctcaggc ggcacaaccg gcctgtgcat cggccacata
     1801 gctcccgaag cggtggacgc aggtcccatc gccttcgtgc gcgatggtga tctgatacgg
     1861 gtcgatatcg cagctcgctc gctcgaccta cttgtcgagc cggcagagct ggaagctcgc
     1921 cgcaacggct gggctcccct tcctccgcgc tatacccgtg gcgtcctcgc gaaatacacc
     1981 aagctcgtgc actcggcctc cgtgggcgcg gtcacggggt agttcgcgcc ctcccatcga
     2041 tctcgacttc gctcgatcac cgacgcacat aaggaacaac tcatgtccac ggacaccagc
     2101 ccgcgaccca agtcgacggc gccagaaata ctcacggggt cgggcgcaat cctgcgctcc
     2161 ctcgagcacc tcggcatctc cgacgtcttc ggccttcccg gcggcgcgat catgcccttc
     2221 tacgacgagc tcatggcctc cacctcgatc cgccacatcc tggtgcggca cgagcagggc
     2281 gctggccacg cggccgaggg gtacgcgtca tccaccggca agatcggcgt cgcgatcgcg
     2341 accagcggac ccggcgcgac caacctcgtg accgccatcg cggatgccta catggactcg
     2401 gtgccgctgc tcgcgatcac cggccaggtc ttctccactt cgatgggaac cgacgcgttc
     2461 caggaggtcg acatcgtggg aatcacgatg ccgatcacca agcactcgtt ccttgtgacg
     2521 cgcgccgagg acgttccggc gacgctggcg gcggcgtacc acatcgccac gaccggccgc
     2581 ccgggcccgg tactcgtcga cgtgaccaag gacgcgcaac agaagagcgc accgttcatc
     2641 tggccgccca aggtcgacct gcccggctac cgcccggtga tgaaggcgca cggcaagcag
     2701 gtgcaggccg cggcccagct gctggtcgag agcgagcgtc cggtgttcta cgttggcggc
     2761 ggcgtcatcc gctcgggggc atcgaaggag ctactggctc tcgcgaccgc ggtcggtgca
     2821 ccgatcgtga cgacgctcat ggcgcgcggc gcattccccg actccaaccc gctcaacctc
     2881 gggatgcccg gcatgcacgg taccgtgccc gcggtgctcg ggctgcagga gtccgacctg
     2941 atcatctcgc tgggcgcccg cttcgacgac cgcgtcacgg gcaaggcgag cgagttcgcc
     3001 cccaacgcga agatcgtgca cgtcgacatc gatccggccg agatcggcaa gatccgcgcc
     3061 gccgatgtgc ccatcgtggg cgacgcgaag gacgtgatcg tcgacctgac cgcggcgttc
     3121 gccgaggcat ccgcaagtca gaagcccgat tacaccgcgt ggtggacacg gctcaacgag
     3181 ctcaagaaga actacccgct cggctacacg atgcccgacg acggactgct ctcgccgcag
     3241 aagatcatcg agcgcatcgg tcagctctcc ggccccgagg cgatctatgc cgcgggtgtc
     3301 ggccagcacc agatgtgggc tgcgcagttc atcaagtacg agcgccccaa cgcctggctg
     3361 aactccggcg gcgccggcac gatgggctac ggagtgcccg ccgcgatggg tgccaaggtc
     3421 gccaaccccg accgcgtggt gtgggcgatc gacggtgacg gatgcttcca gatgaccaat
     3481 caggagctcg ccacctgcac gatcaacaac atcccgatca aggtcgcgat catcaacaac
     3541 tcgtcgctcg gcatggtgcg gcagtggcag accctgttct acgacggacg ccactcgttc
     3601 accgacctca acaccggtca cgacacggtg atgatccccg acttcctcaa gctcgccgaa
     3661 gcctacggct gcctggcgat ccgggtgcgc accgaggacg aagtggatgc cgccattagg
     3721 ctcgccctgg agaccaacga ccgccccgtg gtgatcgact tcatcgtcag ccgcgacgcg
     3781 atggtgtggc cgatggtgcc gcagggcgtc ggcaactcga gcgtgcagta cgcccgagat
     3841 catgcgcccg agtgggagga ggagtaacca tgtcgcacgt tctctccctg cttgtcgaag
     3901 acaagcccgg cctactcacc agggtcgccg gactgttcgc ccgccgcggg ttcaacatcg
     3961 agtcgcttgc cgtgggcaag agcgagatcg agggcctgtc gcgcatcacg gtggtcgtcg
     4021 acgtcgagca gcttcccctc gagcaggtca ccaagcagct caacaagctc gtcaacgtga
     4081 tcaagatcgt ggagctcgac cccacgcagt cggtgacgcg cgagcacctg ctcatcaagg
     4141 tgcgcgtcga caacaccacc cgatctcaga ttctcgaggc cgtcaccctg ttcagggcca
     4201 aggtggtgga tgtcgccacc gacgccctcg tgatcgagac cacgggcgac acccccaaga
     4261 tccaggcctt cctgcgcgtg ctcgagccct acggcatcaa ggagatcgcg cagtcgggtc
     4321 tgctcgccat cggtcgcggc agcaagagca tcaccgaacg cgtcttcaag aactgacaac
     4381 caaccggtga tcgagcgaag tcgagatcag gtctcgacac gctcgatcac cgaacacaca
     4441 caacgaagga acgaaacagt ggcagcagaa ctcctctacg acaacgacgc cgacatctcc
     4501 ctcatccagg gcaagaaggt cgccatcatc ggttacggct cgcagggcca cgcccacgcg
     4561 cagaacctgc gcgactccgg cgtcgaggtg aagatcggac tcaaggacgg ctccaagtcc
     4621 gcgcccaagg ccaccgagga gggcttcgag gttctctccg tcgccgacgc cgccgagtgg
     4681 gccgacgtga tcatgatcct cgcgcccgac cagcaccagc gcgccatcta cagcggcagc
     4741 atcaaggaca agctgaagcc gggcaagacc ctcgccttcg cgcacggctt caacatccgc
     4801 ttcggctaca tcgacgcccc cgagggtgtc gacgtgatcc tgatcgcgcc gaaggccccc
     4861 ggtcacacgg tgcgccgcga gttcgtcgcg ggtcgcggca tcccggacat catcgccgtc
     4921 gagcgcgacg cctcgggcca cgcctgggac ctcgccctct cgtacgcgaa ggcgatcggt
     4981 ggcacccgcg ccggcgtcat caagaccacc ttcaccgagg agaccgagac cgacctgttc
     5041 ggtgagcagg ccgtgctctg cggtggcacc tcgcagctgg tcatgtacgg cttcgagacg
     5101 ctcatcgagg ccggctacca gcccgagatc gcctacttcg aggtgctgca cgagctcaag
     5161 ctcatcgtcg acct
//
LOCUS       DAOSOU010000159         5168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_16195_length_5168_cov_6.414238, whole genome shotgun sequence.
ACCESSION   DAOSOU010000159 DAOSOU010000000
VERSION     DAOSOU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16195_length_5168_cov_6.414238"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLW15_08740"
     CDS             <1..386
                     /locus_tag="PLW15_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT34518.1"
                     /translation="RLLKLPASVQKRVAAGVLSAGHARAVLAAGDAAAMERLADKIVN
                     EDLSVRAAEAAAGQVATRTPKAKPVAGKRQGQLDEVAERLGDALNTRVKVTLGASKGQ
                     IVIDFATVADLNRILGELGQQGFSR"
     gene            390..872
                     /locus_tag="PLW15_08745"
     CDS             390..872
                     /locus_tag="PLW15_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT34519.1"
                     /translation="MVGSVEIVRADPTSDEVLPLLQEHLVDMYATSPAESVHALDPEQ
                     LRHPSITFFVARDGTEVVGTGAIRELSATEGELKSMRTSAGARGRGVGAAMLEHLLAE
                     AHTRGYRALRLETGVEDYFAPARRLYARYGFVEGEPFADYWADPNSVFMHLELEAPRV
                     "
     gene            complement(869..1822)
                     /locus_tag="PLW15_08750"
     CDS             complement(869..1822)
                     /locus_tag="PLW15_08750"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOT34520.1"
                     /translation="MATPHVLVLAGGISHERDVSLRSGRRVADSLAEHGLTVELRDPD
                     ASLLSYLRTNRPDVVWPALHGASGEDGALRGLLDFIGVPYVGSRAEASRLAWDKPTAK
                     TIVGRVGVSTPRSITLPRDAFRELGANSILEEIQDEFSGPLVVKPAQGGSAQGVTMVD
                     DRSLLPRAMVDAYTYCDSALIEQKITGTEIAIGIIDSGDGPVALPAVEIQPLSGVYSF
                     EARYNAGETRFFVPARIDPDVAARAEQAALDAHSALGLRHLSRVDLIVDGAGTPWFLE
                     ANVLPGLTETSILPQALEAAGHDLGWVYSALADAAIAESRA"
     gene            complement(1822..3126)
                     /locus_tag="PLW15_08755"
     CDS             complement(1822..3126)
                     /locus_tag="PLW15_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT34521.1"
                     /translation="MTNQGNNLDKWYGHYADRTAGLAASEVRALFAVASRPEVVSLAG
                     GMPYVSALPQDLVTSAMERVMRDNGPVALQYGSGQGMPALREHILEVMALEGIRGSVD
                     DIVVTTGSQHALELVTKLFINPGDVVLAEGPSYVTAMVVFKSFQADIQHVPMDDYGLI
                     PQALREHIARLRADGKALKFLYTVPTFSNPAGVTLTWERRLEILEICRANNILVLEDN
                     PYGLLYFDGQPPHAMRSVEEEGVIYLGTFSKTLAPGFRVGWVLAPHAIREKLILANEA
                     AVLSPSSFTQHVIAEYLSTADWKAQIDTFRGVYRERRDAMLTALDDYLPDLSWTHPTG
                     GFYVWVTLPDNLDSKSMLPRAVKELVAYTPGTAFYADGGGRNKMRLAFCYPTPDFIRE
                     GIRRLSTVINGELELLSTFSQTAPLPVVRPEPTMINPPANLG"
     gene            complement(3214..3693)
                     /locus_tag="PLW15_08760"
     CDS             complement(3214..3693)
                     /locus_tag="PLW15_08760"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOT34522.1"
                     /translation="MKRSLPWVLVVAQFALLAALAFVPQSSLWPVGTPALIAAIVLVV
                     GGGVIALLGARGLGPALTATPVPKERSALVTTGLYSRVRHPIYTGLLIVGLGLVVLGS
                     SVVHIVLWLALLVLLAVKARWEERMLAATYPDYDAYAATTGRFIPGIGRVRQRPGQS"
     gene            complement(3788..4114)
                     /gene="trxA"
                     /locus_tag="PLW15_08765"
     CDS             complement(3788..4114)
                     /gene="trxA"
                     /locus_tag="PLW15_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT34523.1"
                     /translation="MSTAHDVTDATFADEVLNSKDTIMVDFWAEWCGPCRAVSPILDQ
                     IAAEHADKIKIVKLNVDDNPQMAMKYQITSIPAMKVFRGGEVVKTVIGAKPKPAIEAD
                     LAEFLA"
     gene            complement(4135..5103)
                     /gene="trxB"
                     /locus_tag="PLW15_08770"
     CDS             complement(4135..5103)
                     /gene="trxB"
                     /locus_tag="PLW15_08770"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT34524.1"
                     /translation="MRQVIIIGSGPAGYTAAIYAARASLAPLLIASSVEAGGELMKTT
                     EVENYPGFAEGIQGPELMMAMQAQAERFGTEIVLDDVVSLELEGDIKRVTLGNGDVHE
                     ALAVIFATGSAYRKLGLEDETRLSGHGVSWCATCDGFFFRQKTIAVVGGGDSAMEEAT
                     FLTKFADKVYVIHRKDSLRASKAMQERAFSDPKIEFLFNKEVAHIYGDELVTGIGIVD
                     TVDGTETTLDLSGLFVAIGADPRTHLVHGQLDLNPDGTIHVDGRSSRTNIAGVFAAGD
                     VIDPTYRQAVTAAGSGTVAALDTEHYLASLPKNIVDAAHQPAVASA"
BASE COUNT          846 a         1668 c         1828 g          826 t
ORIGIN      
        1 tgcggctcct caagctgccg gcgtcggtgc agaagcgggt agctgccggc gtcctgagtg
       61 cgggccacgc gcgcgccgtg ctcgcggcgg gcgacgcggc cgcgatggaa cgcctcgccg
      121 acaagatcgt caacgaggac ctctcggtgc gggccgccga agcggccgcc ggtcaggtcg
      181 ctactcgcac gccgaaggcg aagcccgtcg cgggcaagcg ccaaggtcag ctcgatgagg
      241 ttgccgagcg actcggcgat gccctcaaca cgcgggtgaa ggtgaccctc ggcgcgagca
      301 agggtcagat cgtcatcgac ttcgccacgg tcgccgacct caaccggatc ctcggtgagc
      361 tgggtcaaca gggcttctcc cggtaagtcg tggtcggctc agtcgagatc gttcgggcgg
      421 atcccacgag cgacgaggtt ctcccgctgc ttcaggagca cctcgtcgac atgtacgcga
      481 cgtcacccgc cgagagcgtc cacgctctgg atccggagca gctgcggcat ccctcgatca
      541 cgttcttcgt ggctcgggat ggtacagagg tcgtcggcac cggcgcgatc cgcgagctga
      601 gtgcgacgga gggcgaactc aagtcgatgc gcaccagcgc cggggctcgc ggtcgcgggg
      661 tcggtgccgc catgctcgag catctgctgg ccgaggcaca cacccggggc tatcgggcgc
      721 tgcgtctcga gaccggcgtc gaggactact tcgcccccgc ccgacggttg tacgcccggt
      781 acggcttcgt cgagggggag cccttcgccg actactgggc cgaccccaac agcgtcttca
      841 tgcacctcga gctcgaggcg cctagagtct aggcgcggct ctcggcgatc gcggcatccg
      901 ccaacgcgga gtacacccag ccgaggtcgt gcccggccgc ctcgagggcc tggggcagga
      961 tcgacgtctc ggtgaggccg gggagcacgt tggcttcgag gaaccacgga gtgcccgccc
     1021 cgtcgacgat gaggtcgaca cgggagaggt gcctgagccc cagggcgctg tgtgcgtcca
     1081 gtgcggcctg ctcggcgcga gccgcgacat ccggatcgat gcgggccggc acgaagaagc
     1141 gggtctcccc cgcgttgtag cgcgcctcga agctgtacac gcccgatagc ggttggatct
     1201 cgaccgcggg cagggccacg gggccgtcgc cgctgtcgat gatgccgatg gcgatctcgg
     1261 tgccggtgat cttctgctcg atgagcgccg agtcgcagta cgtgtacgcg tcgaccatcg
     1321 cgcgcggcaa cagcgagcga tcgtcgacca ttgtcacccc ctgagccgag ccaccctgcg
     1381 ctggcttgac caccagcggg cccgagaact cgtcctggat ctcctcgagg atgctgttgg
     1441 ccccgagctc gcggaacgcg tcgcgcggca gggtgatcga ccgtggggtg ctgacgccca
     1501 cccggccgac gatggtcttc gcggtcggct tgtcccaggc cagccgggat gcctcggcgc
     1561 gagaacccac gtagggcacc ccgatgaagt ccaggaggcc gcggagcgct ccgtcctccc
     1621 cactggcccc gtggagggcg ggccagacca cgtcgggtcg gttggtgcgc aggtagctca
     1681 ggagtgaggc atccggatcg cggagttcga ccgtgaggcc gtgctctgcg aggctgtccg
     1741 caacccggcg cccggagcgc agcgagacgt cgcgctcgtg ggagattcca ccggcgagga
     1801 ccagtacgtg cggggtcgcc atcagccgag gttggcgggt gggttgatca tcgtgggctc
     1861 ggggcgcacg acgggcaggg gcgcggtctg cgagaacgtg ctgagcagtt cgagctcgcc
     1921 gttgatgacc gtcgacaggc gacggatgcc ctcgcggatg aagtcgggcg tcgggtagca
     1981 gaatgccaaa cgcatcttgt tgcgcccgcc gccgtcggcg tagaacgcgg tgccgggcgt
     2041 gtaggcgacc agctccttga cggcgcgggg cagcatcgac ttggagtcga ggttgtcggg
     2101 cagggtcacc cagacgtaga agcccccggt ggggtgggtc cagctgaggt cggggaggta
     2161 gtcgtcgagg gcggtgagca tcgcgtcgcg tcgctcacgg tagacgccgc ggaaggtgtc
     2221 gatctgagcc ttccagtcgg cggtcgacag gtactcggcg atgacgtgct gggtgaacga
     2281 gctcggagag agcaccgccg cctcattcgc caaaatgagc ttctctcgaa tagcgtgggg
     2341 ggcaagcacc catccgacac ggaacccggg agcaagggtc ttggagaatg tgccgaggta
     2401 gatcacgccc tcctcctcga ccgagcgcat ggcgtgcggg ggctgaccgt cgaagtagag
     2461 caggccgtag gggttgtcct cgagcacgag gatgttgttc gcccggcaga tctcgaggat
     2521 ctcgaggcgc cgctcccagg tcagagtcac gccggccggg ttgctgaacg tcggaaccgt
     2581 gtacaggaac ttcagcgcct tgccgtccgc acgaagacgg gcgatgtgct cgcggagcgc
     2641 ctgagggatc agtccgtagt catccatggg cacgtgctgg atgtcggcct ggaacgactt
     2701 gaacacgacc atggccgtca cgtagctcgg gccctccgcc agcaccacgt cgccggggtt
     2761 gatgaagagc ttggtgacca gttcgagcgc atgctgcgat ccggtcgtca cgacgatgtc
     2821 gtcgacgctg ccccggatgc cctcgagcgc catcacctcg aggatgtgct cgcgcagggc
     2881 gggcatcccc tgaccggaac cgtactgcag ggcgaccggg ccgttgtcac gcatgacgcg
     2941 ttccatcgcc gacgtcacca ggtcctgggg cagggccgac acgtagggca tgccaccggc
     3001 gagcgagacg acctcgggac gggaggcgac ggcgaacagc gcgcgcacct ccgaggcggc
     3061 gaggccggcc gtgcgatcgg cgtagtggcc gtaccacttg tcgagattgt tgccctggtt
     3121 ggtcatgcgc tgcccttttc agtcgcgaag cttcaagaat agggcagggt caccacgaaa
     3181 acaccgcccc agagccgggg acggtccagt gcgtcaggat tggccggggc gctggcgaac
     3241 ccgaccgatg cccgggatga agcggccagt ggtcgcggca taggcgtcgt agtcggggta
     3301 ggtcgccgcg agcatccgct cctcccagcg ggccttgacc gcgagcagca cgagcagcgc
     3361 cagccacagc acgatgtgca ccaccgagga gccgagcact accagcccca gcccgacgat
     3421 cagcagcccg gtgtagatgg gatgccgcac ccgcgagtac aggcccgtgg tgaccagcgc
     3481 cgagcgctcc ttgggcaccg gcgtcgcggt gagagccggt ccgagaccgc gggcgccgag
     3541 cagcgcgatg accccgccgc cgaccacgag cacgatggcc gcgatcagcg cgggagtccc
     3601 caccggccac aacgagctct ggggcacgaa ggccaaagcc gcgagaaggg cgaattgggc
     3661 gaccacgagc acccacggaa gcgaccgttt catgggccga gactacgtga acgagacacc
     3721 gcgcggcatc ctgacagcac gaagccccgg caaccgcgag ggcggccggg gcttcgcgtg
     3781 gatcggatca ggccaggaac tcggccaggt cggcctcgat ggccggcttc ggcttcgcac
     3841 cgatgaccgt cttgacgacc tcgccgccgc ggaagacctt catcgcgggg atcgacgtga
     3901 tctggtactt catcgccatc tgcgggttgt cgtcgacgtt gagcttgacg atcttgatct
     3961 tgtcggcgtg ctcagccgcg atctggtcga ggatcgggga gacggcgcga cacgggccgc
     4021 accactcggc ccagaagtcc accatgatgg tgtccttcga gttgagcacc tcatcggcga
     4081 acgtggcgtc ggtgacgtcg tgtgctgttg acatgggttt ccttctttcg tgaatcaggc
     4141 ggatgcgacg gccggctggt gtgccgcgtc gacgatgttc ttcggaagcg aagcgaggta
     4201 gtgctcggtg tcgagcgccg ccacggtgcc cgaccccgcg gccgtcactg cctggcggta
     4261 cgtggggtcg atgacatcac cagcggcgaa aacgccggcg atgttggtgc ggctggagcg
     4321 gccgtcgacg tggatggtgc cgtccgggtt gaggtcgagc tggccgtgca cgaggtgcgt
     4381 gcgcgggtcg gcgccgatcg ccacgaacag gccactcagg tcgagggtgg tctccgtgcc
     4441 atccacggtg tcgacgatgc cgatgcccgt cacgagctcg tcaccgtaga tgtgagcgac
     4501 ctccttgttg aacaggaact cgatcttcgg gtcgctgaac gcacgctcct gcatcgcctt
     4561 ggaggcccgc aggctgtcct tgcggtggat cacgtagacc ttgtcggcga acttcgtgag
     4621 gaaggttgcc tcctccatcg ccgagtcgcc gccaccgacg accgcgatgg tcttctggcg
     4681 gaagaagaat ccgtcacagg tggcacacca cgagacgccg tgaccgctca ggcgcgtctc
     4741 gtcctcgaga ccgagcttgc ggtaggcaga gcccgtcgcg aagatcaccg cgagcgcctc
     4801 gtgcacgtct ccgttgccga gagtcacgcg cttgatgtcg ccctcgagct ccaggctcac
     4861 gacgtcgtcg aggacgatct ccgtgccgaa gcgctcggcc tgcgcctgca tcgccatcat
     4921 gagctcggga ccttggatgc cctcggcgaa tcccgggtag ttctcgacct cggtggtctt
     4981 catgagctca ccgccggcct cgacggagct cgcgatgagc agcggcgcga ggctggcacg
     5041 agccgcgtag atggccgccg tgtagccggc cgggccggat ccgatgatga ttacttgccg
     5101 cacttgacgc gtctccctca gggttatcac tgcccggtac aacacatact atgtcgaggc
     5161 tattcctg
//
LOCUS       DAOSOU010000160         5157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_16240_length_5157_cov_5.581145, whole genome shotgun sequence.
ACCESSION   DAOSOU010000160 DAOSOU010000000
VERSION     DAOSOU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16240_length_5157_cov_5.581145"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLW15_08775"
     CDS             complement(<1..604)
                     /locus_tag="PLW15_08775"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT34525.1"
                     /translation="MRILVTGGAGFIGSNFVRRTLEDAYPGLEGADVVVLDALTYSGN
                     LENLAPVADSPRYEFVHGDIRDADLLDRILPGVDAIVHFAAESHVDRSVRDSGIFVET
                     NVLGTQRLLDAALRNGTARFVHVSTDEVYGSIAEGSWDESRPLEPNSPYSASKAGSDL
                     LARSYFRTHGLNLSITRCSNNYGPYHFPEKVIPLFVTNLID"
     gene            complement(610..>1455)
                     /locus_tag="PLW15_08780"
     CDS             complement(610..>1455)
                     /locus_tag="PLW15_08780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34526.1"
                     /translation="LSQEGVRVRVVALDDESSDGTRDWLVERAAHEPRLTVLPSMGPS
                     GRSAANFYRLIALVEPPADGYLAFSDQDDIWLPGKLARHVELLRSGGHDGISSSVTAF
                     SSSGERTVIRKDYPQRRFDYLTESPGPGCSFLLSPRLAALVAAQLADDDSPARAAEYH
                     DSLIYALARGRGWSWHIDGVPSLDYRQHDSNVMGANTGLSPALTRLRLIREQWHRTQA
                     IIHARSALRVATPDTRAGLEQMLRLLEGRGIRARWTLASHAGQMRRRPRDRAIIRVLI
                     ATGVW"
     assembly_gap    1456..1555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1556..2380
                     /locus_tag="PLW15_08785"
     CDS             <1556..2380
                     /locus_tag="PLW15_08785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34527.1"
                     /translation="VRESPLPRPELLVLRNVPALGVVANFEQAVRACTGALIALCDQD
                     DVWMPDKLARFVECFTAEPDLLLAHSDATLVTADRTPLPDSLLDSLEATPGERRHLVD
                     GRSYYTLIRRNLVTGATVVFRRELLEHAVPFAPSWVHDEWLAIVASAISRTLLIDGRL
                     IEYRQHGGNQIGASKPSLRYKFGKLREPRLERNQGLVARSTALVERLESLGTLIDSSR
                     LAHARAKLTHEKWRLALPSNPVLRAPRIAVAVVAGRYSRFSRGLMDAARDLLQPAD"
     gene            2403..3005
                     /locus_tag="PLW15_08790"
     CDS             2403..3005
                     /locus_tag="PLW15_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOT34528.1"
                     /translation="MQIRELAVPDAWEITPVPWKDDRGLFLESFRHDRLAEATGRSFE
                     LQQANVSVSRRGVVRGIHFADTPPGQAKYVSVARGAGIDFIVDIRVGSPTFGTWDSVL
                     LDADSRRSVFISEGLGHAFAALTDDATLTYLVSSTYNPAAEHDLNILDPDIGLVFPSE
                     LGEPVLSPKDLAAPSLAELLASGGLPDYAELRAYYAKATA"
     gene            3007..3876
                     /gene="rfbA"
                     /locus_tag="PLW15_08795"
     CDS             3007..3876
                     /gene="rfbA"
                     /locus_tag="PLW15_08795"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOT34529.1"
                     /translation="MRGIILAGGSGTRLWPITKGISKQLMPVYDKPMIYYPLSTLMSA
                     GIREILIITTPDDQVAFRRLLGDGSELGISISYAVQPSPDGLAQAFIIGEEFIGSEKV
                     ALVLGDNIFHGAGLGSNLRSHTDVDGALIFAYQVANPRAYGVVEFDSEHRAVSIEEKP
                     EHPKSNFAVPGLYFYDNSVVGIARSITPSARGELEISTVNEHYLTSGALRVDVLDRGT
                     AWLDTGTFESMVQASEFVRVIEARQGYKIGCIEEIAWRAGWIDDVQLVALSRPLVKSG
                     YGEYLERLLAEQR"
     gene            3873..>5157
                     /locus_tag="PLW15_08800"
     CDS             3873..>5157
                     /locus_tag="PLW15_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT34530.1"
                     /translation="MTRVLVITGDPIGPKMAGPAIRAWNMAQLLAIDNEVALMTTTLL
                     EPVDASFELHRVKPGEDAVFARLLAWADVIVFQGHAMAQFDALRTTEKIVVADIYDPM
                     HLEMLEQGRELGRATWELRVTTATRVLNEQLALADFFLCASERQRLFYLGQLTTLGRV
                     NPATYENDPHLEQLIAVAPFGLSSTPPAHTTDVVKGVRSGVAVDDKLLIWGGGLYNWF
                     DPLTLITAVAQLAERRPAVRLLFLGTKHPGVDEMGIVRESFDLARELGALDTSVFFNE
                     SWVQYADRQSWLMEADAGVSTHMSHVETTFSFRTRILDYLWAGLPMVVTEGDSFAELV
                     EREGLGVVVPARDVAALELALERVLFDEEFIAQTRRNVERVRADFLWEKTLAPLVEFV
                     ANPHRAADSVGSRVDRSASGKRRKPYGLAHDIRMFW"
BASE COUNT          824 a         1664 c         1703 g          866 t
ORIGIN      
        1 catcgatgag gttggtgacg aacagcggga tgaccttctc ggggaagtga tacggcccgt
       61 agttgttgct gcaccgggtt atcgagaggt tgagtccatg ggtgcggaag tagctgcgag
      121 ccagcaggtc gctgcccgcc ttcgaggccg agtacggcga gttgggctcg agcggtcgcg
      181 actcgtccca tgagccctcg gcaatggatc catagacctc gtcggtggag acgtgcacga
      241 accgagcggt gccgttgcgc agggccgcat ccagcagccg ctgggtgccg agcacgttgg
      301 tctcgacgaa gatgcctgag tcgcgcaccg agcggtcgac gtgggattcg gcggcgaagt
      361 gcacgatcgc gtccacgccc ggcaggatgc ggtcgagcag gtcggcgtcg cggatgtcgc
      421 cgtgcacgaa ctcgtatcgc ggcgagtcgg ccacgggtgc caggttctcg aggttccccg
      481 agtaggtgag ggcgtcgagc acgaccacgt cggcgccttc gaggcccggg taggcgtctt
      541 cgagggtgcg ccgaacgaag ttcgagccga tgaagccggc accgccggtt accaggattc
      601 tcattgccgt caccacacac ccgtcgctat caagactctg attatcgcgc ggtcacgggg
      661 tcggcgccgc atctgccccg cgtgggacgc gagcgtccag cgcgcacgga tgcctcgtcc
      721 ctccagcaac cgcagcatct gctcgagccc ggcgcgcgtg tccggcgtgg ccacacgcag
      781 tgccgagcgc gcgtggatga tggcctgagt gcggtgccac tgctcccgga tgagtctcag
      841 gcgcgtcaat gccggactga gaccggtgtt ggctcccatc acgttggagt cgtgctgccg
      901 gtaatcgagg ctcggcacgc cgtcgatatg ccacgaccag ccccgaccgc gagcgagagc
      961 gtagatcagt gagtcgtggt actccgctgc tctcgcgggt gagtcgtcgt cggcgagttg
     1021 cgccgccacc agggcggcaa gtcgtgggga caacaggaac gagcatccgg gccccgggct
     1081 ctcggtcagg tagtcgaagc gacgctgggg gtagtccttg cggatcacgg tgcgctcgcc
     1141 gctcgaggag aatgcagtga ctgaggagga gatgccgtcg tgcccgcccg acctcaggag
     1201 ttcgacgtgc cgtgcgagtt tgccgggtag ccagatgtcg tcctggtcgc tgaaggcgag
     1261 gtagccgtcg gctggtggct cgacgagggc gatgagccgg tagaagttag ctgccgaccg
     1321 gccggacggg cccatcgagg gcagaacggt caggcgcggc tcgtgcgccg cccgctcgac
     1381 gagccagtcc cgggtgccgt cggatgattc atcgtcgagg gcgacgacgc gcacccgaac
     1441 accctcttgg gagagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtacg
     1561 tgagtcgccg ctgccgagac cggagctact cgtgctgcgc aacgtgcccg ctctgggagt
     1621 ggtcgccaac ttcgagcagg cagtgcgggc gtgcacggga gccctcatcg cgctgtgcga
     1681 tcaggacgac gtctggatgc ccgacaagct cgcccggttc gtcgagtgct tcaccgccga
     1741 acccgacctg cttctcgcac acagtgacgc gaccctcgtg accgccgatc ggacacccct
     1801 gccggactcc cttctggaca gccttgaggc cactccggga gagcggcggc acctcgtgga
     1861 cgggcgcagc tactacacgc tcatccgacg caacctcgtg accggcgcga ccgtggtgtt
     1921 ccgtcgcgag ctgctcgagc acgccgtgcc attcgcgccg tcgtgggtgc acgacgagtg
     1981 gctcgcgatc gttgcctccg cgatcagccg tactctcctg atcgacgggc ggctcatcga
     2041 gtaccgccag cacgggggca accagatcgg ggcgagcaag cccagcctca ggtacaagtt
     2101 cggcaagctg cgcgaaccgc gcctggagcg caaccagggg ctcgttgctc gctcgaccgc
     2161 gctcgtggag cgactcgaat cgctcggtac cctcatcgac tcatcccggc tcgcgcacgc
     2221 cagggcgaag ctcacacacg agaagtggcg actcgccctg ccgtccaacc cggttctgcg
     2281 tgccccgcga attgccgtcg cggttgtggc tgggcgctac agccgattca gccgcggcct
     2341 catggacgcc gcccgcgacc tcctccaacc tgccgactga gcgcgcacgt taggatcgtc
     2401 ccgtgcagat tcgagaactc gcggtgccgg atgcatggga gatcaccccg gtgccctgga
     2461 aagacgatcg gggtctgttt ctcgagtcct tccggcacga ccggctcgcc gaggcgaccg
     2521 ggcgctcctt tgaactgcag caggccaatg tctcggtctc gcgccgaggt gtcgtgcgcg
     2581 gcatccactt cgcagacact cctcccgggc aggccaagta cgtgtcggtg gcccggggag
     2641 cgggcatcga cttcatcgtc gacatccggg tcggctcgcc gacgttcggc acctgggact
     2701 cggtcctgct cgatgccgac tcgaggcggt cggtgttcat ctccgagggg ctcggccacg
     2761 cattcgccgc attgacggat gacgccaccc tcacctacct cgtgagctcg acctacaacc
     2821 cggccgccga acacgacctc aacattctcg accccgacat cggacttgtg ttcccgtcgg
     2881 agctcggcga gcccgttctc agccccaagg acctcgcggc cccgtcgctg gccgagttgc
     2941 tcgcgtcggg tggactcccc gactacgcgg aactgcgggc gtactacgcg aaagccacgg
     3001 cctgacgtgc gcggcatcat ccttgccggc ggcagcggca cgcggctgtg gccgatcacc
     3061 aagggcatct ccaagcagct gatgcctgtg tacgacaagc cgatgatcta ctacccgctg
     3121 tcgacgctca tgagcgcggg catccgggag atcctgatca tcaccacccc cgacgaccag
     3181 gtggcgtttc gccggttgct gggtgacggc agcgagctcg ggatctccat ctcctacgct
     3241 gtgcagccgt cgccggatgg tctcgcgcag gccttcatca tcggcgagga attcatcggc
     3301 tctgagaagg tcgcgctcgt tctgggcgac aacatcttcc acggcgcggg gctcggctcc
     3361 aacctgcgca gccacaccga cgtcgatggt gcgttgatct tcgcgtacca ggtggccaac
     3421 ccgcgcgcct acggtgtggt cgaattcgat tccgagcaca gggcggtgtc gatcgaggag
     3481 aagccggagc atccgaagag caacttcgcg gtgccggggc tgtacttcta cgacaactcg
     3541 gtcgtgggta tcgccaggtc gatcacgccg agcgcccggg gggaactcga gatctccacg
     3601 gtcaacgagc actacctcac gtccggcgca ttgcgcgtcg acgtgctcga ccggggcact
     3661 gcgtggctcg atacgggaac cttcgagtcg atggtgcagg cgagtgagtt cgtgcgggtc
     3721 atcgaggccc gtcagggcta caagatcggc tgcatcgagg agatcgcgtg gcgagcgggc
     3781 tggatcgacg acgtgcagct cgtcgccctg tcccggcctc tggtcaagag cggctacggc
     3841 gaatacctcg agcggctgct cgcggagcaa cggtgacgcg agttctcgtc atcacgggcg
     3901 acccgatcgg gccgaagatg gcgggtccgg ccatccgggc ctggaacatg gcgcaactgc
     3961 tggccatcga caacgaggtc gcgttgatga cgacgaccct cctggagccg gtcgatgcat
     4021 cattcgagct ccaccgcgtc aagcccggtg aggatgccgt gttcgcgcgc cttctcgcct
     4081 gggccgacgt catcgtcttc cagggccacg ccatggcgca gttcgacgct ctgcgcacca
     4141 ctgagaagat cgtcgtcgcc gacatctacg acccgatgca tctggagatg ctggagcagg
     4201 gtcgcgagct cggtcgtgcg acctgggaac tcagagtcac gaccgccacc cgggtgctca
     4261 acgagcagct cgcgctcgcc gacttcttcc tgtgcgcctc cgaacgccag cgcctgttct
     4321 atctcggaca gctgacgacg ctggggcgcg tcaaccccgc gacgtacgag aacgatcccc
     4381 acctcgagca gttgatcgcg gttgcaccgt tcggcctgag ctccacaccg cccgcccaca
     4441 ccacggatgt ggtgaagggt gtgcgcagcg gggtcgccgt cgacgacaag ctgctcatct
     4501 ggggcggcgg gctgtacaac tggttcgacc cgctgacgct gatcacggcg gtggcacagt
     4561 tggccgagcg acgcccggct gtcaggctgc tgttcctggg aacgaagcat ccgggggtcg
     4621 atgagatggg tatcgtgcgc gagtcgttcg atctcgcccg tgaactgggg gcgctcgaca
     4681 cctcggtgtt cttcaacgag tcatgggtgc agtacgccga tcggcagagc tggctcatgg
     4741 aggccgacgc gggcgtgagc acgcacatgt cccacgtcga gaccacgttc tcgttccgca
     4801 ctcgcatcct ggactacctc tgggccggac tgcccatggt cgtgaccgag ggcgacagct
     4861 tcgccgagtt ggtcgagcgc gagggactcg gggtcgttgt gccggcacgg gatgtcgcgg
     4921 ccctcgaact ggccctcgag cgtgtgctct tcgatgagga gttcatcgcg cagacgcgac
     4981 gcaacgtcga gcgcgtgcgc gctgacttcc tctgggagaa gaccctcgca ccgctcgtgg
     5041 agttcgtggc gaatccccat cgtgccgccg acagcgttgg aagccgagtc gaccgctcgg
     5101 cgtcgggaaa gcgtcgcaag ccctacggac tcgcccacga cattcggatg ttctggc
//
LOCUS       DAOSOU010000161         5126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_16403_length_5126_cov_6.023270, whole genome shotgun sequence.
ACCESSION   DAOSOU010000161 DAOSOU010000000
VERSION     DAOSOU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16403_length_5126_cov_6.023270"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="PLW15_08805"
     CDS             complement(<1..1433)
                     /locus_tag="PLW15_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT34531.1"
                     /translation="MANPRVHEIAAELGIESKIVLDKLKDIGQFVKGPSSSVAPPVAR
                     KVKEALIADGVKPAAPAEKPKAAPKAAPRPAAPAPAAPAADDYDVDAPAPAAPPLTVA
                     ERQAQAEALAQAQAQAQAAQAQTATPAPTEAAAASTDAAAPSPAGIPRPGTPRPGNNP
                     FASNQGMQRPGAPRPGNNPFASNQGMQRPGTPGAIPRPGAPRPGGPGQGARPAAFGQR
                     PGGNAPFQQRPGGPGRPAGGGGFRPGGAPGGAPGNNFGPNRPPAGGAGGRGRGPGGGT
                     AGAFGRGGGKSKARKSKRTKRAEFELREAPSLGGVSVPRGDGKTVIRLRRGASITDLA
                     DKLETLTGYMVQPGSLVTALFHLGQMATATESLDEETFSILAGELNYKIEMVSPEDED
                     RELLAGFDLDLDQELEDESDEDLEIRPPVVTVMGHVDHGKTKLLDAIRNANVVAGEAG
                     GITQHIGAYQVWTEHEGHERAITFIDTP"
     gene            complement(1524..1862)
                     /locus_tag="PLW15_08810"
     CDS             complement(1524..1862)
                     /locus_tag="PLW15_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOT34532.1"
                     /translation="MPGSTSSPTRYLRTSTAPRGFRLVPVRTCIGCRVRAEASLLLRV
                     VARDGEVVPDPSATLPGRGAWVHPTAHCVEISAKRRAFGRALRVSSALDASQVLALVH
                     SQPGTEEQAD"
     gene            complement(1819..2838)
                     /gene="nusA"
                     /locus_tag="PLW15_08815"
     CDS             complement(1819..2838)
                     /gene="nusA"
                     /locus_tag="PLW15_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT34533.1"
                     /translation="MDIDLSVLRLMEREREIPFEELVQIIEQAILTAYLKHVGLLDQK
                     AQPEHKSTDATSLKARVELDRKTGKVTIWVPELDDEGAVIGEAEDSPDDFGRIAAFAA
                     KQVINQRLRDIADDHVLGEFKGREGDIVAGVIQQGPNPRMIHVDLGTVEAILPPEEQV
                     PGEDYAHGTRIRVYVTAVSKGLKGPQITVSRTHPALVRKLFALEVPEIASGIVEITSL
                     AREAGHRTKMAVRALEPGVNAKGACIGELGQRVRAVTAELNNEKIDIVDYSDNLATFV
                     ANALSPAKVTSSYVIDESTKAVRALVPDYQLSLAIGKEGQNARLAAKLTGARIDIQPD
                     SILED"
     gene            2963..3784
                     /locus_tag="PLW15_08820"
     CDS             2963..3784
                     /locus_tag="PLW15_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit 4 (chain
                     M)"
                     /protein_id="HOT34534.1"
                     /translation="MPLERLLATVALSTIAVIGLSGCVTIVPAAPVPVADGAVPQEQD
                     DDFGTVDVYTVLDDGTLDPAASGLTATVWDTFTRVATLDFAAEVMTQYRVGDSASSDT
                     LAYVYQDDDPQYWVLAANLATSDDPTQLVATLIHEYGHILMLATDEVDPTATSCDTIE
                     LDEGCAHGDSVLLAFQQQFWAPYGDAAPDLANTDADLAWEFYLAHEDDFVSDYAATNA
                     IEDAAETFMTFVLEDEPTGDSMVARKIAFFWGYPELVAIRERIRTEFADDLGLVG"
     gene            complement(3813..4748)
                     /locus_tag="PLW15_08825"
     CDS             complement(3813..4748)
                     /locus_tag="PLW15_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34535.1"
                     /translation="MKPLKTALAAIATLALASAMVLAGSTAAQAYPATPAEVNAVVFT
                     NGLANDTSSGQEVENETAVLTGLGITVTPFDGGDGSADAWIAALAGQDLLVLPELESS
                     DNNPFYEPGGTPWVSDAALQVIKDWVDAGGYVIINGHHSTPDYTPMLLAFTGKDYAPV
                     FTTSNDNTLSWDLQVPLSTSDPLAYVNGTYGLQEYGTWSDDLKSIVTPIYLTTDGQDM
                     AVGTFGVGAGVVIYIGYDWYPDDEDNPGGIPYWGELLELAASGELPFAPQPIYVAPAA
                     PALAATGFDFAPTAAIAGVLLLAGGAFLLLRRRTV"
     gene            complement(4830..>5126)
                     /locus_tag="PLW15_08830"
     CDS             complement(4830..>5126)
                     /locus_tag="PLW15_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOT34536.1"
                     /translation="RNIAPFDVHVIAAGREEVIYETAEALVGSLEESGFDVLFDDRPK
                     VSPGVKFGDAELLGVPQIVIVGRDAAEGRVELWDRRTGERTSIGLDEVGAALLA"
BASE COUNT          794 a         1691 c         1752 g          889 t
ORIGIN      
        1 ggggtgtcga tgaaggtgat ggcgcgctcg tggccctcgt gctcggtcca gacctggtag
       61 gcaccgatgt gctgggtgat gccacccgcc tcaccggcaa ccacgttggc gttgcggatc
      121 gcgtcgagga gcttggtctt tccgtggtcg acatggccca tgacggtcac gaccggcgga
      181 cggatctcca gatcctcgtc gctctcgtct tcgagctcct ggtcgaggtc gaggtcgaag
      241 ccggcgagca gctcgcggtc ctcgtcctcg ggcgagacca tctcgatctt gtagttgagc
      301 tcgcccgcga ggatcgagaa cgtctcctcg tcgagcgact ccgttgcggt cgccatctgg
      361 ccgaggtgga agagcgcggt caccaggctg cccggctgca ccatgtagcc ggtcagcgtc
      421 tccagcttgt cggccaggtc ggtgatcgac gcgccacgac gcagacggat gaccgtcttg
      481 ccgtcaccgc ggggaacgct cacgccaccc agcgacggtg cttccctcag ctcgaactcg
      541 gcgcgcttcg tacgcttcga cttgcgggcc ttgctcttgc cgccgccgcg accgaaggcg
      601 ccagccgtgc caccgccggg accacgacca cgaccaccgg caccacctgc gggtggacgg
      661 ttgggtccga agttgttgcc gggcgcaccg ccgggggcgc caccgggacg gaagccgccg
      721 ccaccagcgg ggcgaccggg tccacccgga cgctgctgga acggggcgtt gccgccggga
      781 cgctgaccga aggccgccgg gcgcgcaccc tggccggggc cgccgggacg cggggctccg
      841 gggcgcggga tggccccggg agtgccggga cgctgcatgc cctggttgga ggcgaagggg
      901 ttgtttccgg ggcgcggtgc gcccgggcgc tgcataccct ggttcgacgc gaaggggttg
      961 ttgccggggc ggggggtgcc aggacgcggg attcccgcgg ggctcggtgc cgcggcatcc
     1021 gtgcttgctg ctgccgcctc ggtgggtgcc ggggtcgcgg tctgtgcctg cgccgcctga
     1081 gcctgtgcct gtgcctgtgc gagcgcctcg gcctgggcct ggcgctccgc gaccgtcagc
     1141 ggcggggcgg ccggtgccgg agcatcgacg tcgtagtcgt ctgctgccgg tgcagcggga
     1201 gcgggggctg cgggccgcgg tgcggccttc ggggcggcct tgggcttctc ggcgggcgcc
     1261 gccggcttga cgccgtcggc gatcagcgcc tccttcacct tgcgggcgac ggggggcgcg
     1321 acgctggacg aagggccctt gacgaactgg ccgatgtctt tcagcttgtc gagcacgatc
     1381 ttggattcga tgccgagttc ggcagcgatt tcgtgcactc gtgggttagc cacaattctc
     1441 ctgtctgggt ctgcacccgg acaggggcag accgttagtt acggacgggt ctcatttcga
     1501 gccgctcatt agttgtccat aggtcaatca gcctgttctt cagttcccgg ttgggagtgg
     1561 actagtgcaa gcacctgcga ggcatccagc gcactggaca ccctgagcgc cctgccgaat
     1621 gctctgcgct tggcggagat ctcgacacag tgcgccgtgg ggtgcaccca cgcgcctcgg
     1681 cccggaagtg tcgcggacgg atccgggacg acctcaccat cccgtgcgac aaccctcagc
     1741 aggagggatg cctcagcgcg gacgcggcag ccgatgcaag ttcttacggg taccagtcta
     1801 aacccacggg gcgcggtgct agtcctcaag tatcgagtcg ggctggatgt cgatccgggc
     1861 accggtcaac ttcgcggcca agcgggcgtt ctgcccttcc ttgccgatcg cgagcgacaa
     1921 ctggtagtcg ggcaccaacg cgcgcaccgc cttcgtggac tcatcgatca cgtacgagct
     1981 ggtcaccttg gccggcgaca gagcattggc gacgaaggtg gcgaggttgt cggagtagtc
     2041 gacgatgtcg atcttctcgt tgttcagctc ggcggtgacg gcccgcaccc gctgacccag
     2101 ctcaccgatg cacgcaccct tggcgttgac tccgggctcc agcgcccgaa ccgccatctt
     2161 ggtgcggtgt ccggcctcgc gggcgagcga cgtgatctcg acgatgccgc tggcgatctc
     2221 gggaacctcg agtgcgaaca gcttgcgcac gagcgcggga tgcgtgcggc tcaccgtgat
     2281 ctgcggaccc ttgagtccct tgctgactgc cgtgacgtag acccggatgc gcgttccatg
     2341 cgcgtagtcc tcgcccggca cctgctcctc gggcggcagg atcgcctcga cggtgcccag
     2401 gtcgacgtgg atcatccggg gattcggtcc ctgctggatg acgccagcaa cgatgtcgcc
     2461 ctcgcggccc ttgaactcgc cgagcacgtg atcgtcggcg atgtcacgca ggcgctggtt
     2521 gatgacctgc ttggcggcga acgcggcgat gcgtccgaag tcgtcggggc tgtcctcggc
     2581 ctcgccgatc accgcaccct catcgtcgag ttcgggcacc cagatggtga ccttgccagt
     2641 cttgcggtcg agctcgacgc gggccttcag cgacgtggca tccgtcgact tgtgctcggg
     2701 ctgtgccttc tgatcaagga ggccgacatg cttgagatag gccgtcagga tggcctgctc
     2761 gatgatctgc acgagttcct cgaaggggat ctcgcgctcg cgctccatga ggcgcagcac
     2821 gctcaggtcg atgtccactg agggcctccg gtattgagat ctggtgctcg aacgaccgat
     2881 gccgccgatg cgacacccag tttagcggtc ggggcgcgga cggcacaccg tcgcaccggc
     2941 cgccctcccc tactgtggag gaatgcccct cgaacgactc ctcgccaccg tcgcactgag
     3001 caccatcgcg gtgatcggtc tgtcgggatg cgtgacgatc gtcccagccg cacccgtacc
     3061 cgtcgcggac ggcgcggtgc cgcaggagca ggacgacgac ttcggcaccg tggatgtcta
     3121 cacggtgctg gacgacggaa ctctcgaccc cgcggcatcc ggcctcacgg cgacggtctg
     3181 ggacaccttc actcgtgtgg ccacgctcga tttcgcagcc gaggtgatga cgcagtaccg
     3241 cgtcggcgat tcagcgtcga gcgacacgct cgcctacgtg taccaggacg acgatcctca
     3301 gtactgggtg ctcgccgcca acctcgcgac gagtgacgat ccgacccagc tggtggcgac
     3361 actcatccac gagtacggac acatcctgat gctggccacc gacgaggtgg acccgaccgc
     3421 gacatcgtgc gacacgatcg aactcgacga gggttgcgcg cacggcgact cggtgctcct
     3481 ggcgttccag cagcagttct gggcgcccta cggcgacgcg gcacccgatc tcgcgaacac
     3541 cgacgccgac ctcgcctggg agttctacct cgcccacgag gacgacttcg tgagcgacta
     3601 cgcggcaacc aacgcgatcg aggatgccgc ggagacgttc atgaccttcg tgctcgagga
     3661 cgagccgacg ggcgactcga tggtcgcgcg caagatcgca ttcttctggg ggtaccccga
     3721 actcgtcgcg atccgcgagc gcattcgtac cgagttcgcc gacgacctcg ggctcgtagg
     3781 gtagccgcgc cgcgtggagt agctgggcct cgtcagaccg tacgacggcg gagcaacagg
     3841 aacgctccgc ccgccagcag gagcactcca gcgattgccg cggtgggggc gaagtcgaac
     3901 ccggtggccg cgagtgcggg ggccgcggga gcaacataga tcggctgggg agcgaacggg
     3961 agctcgccac tcgccgctag ctcgagcagc tcgccccagt acgggatgcc gcccgggttg
     4021 tcctcatcgt cgggatacca gtcgtaaccg atgtagatga cgactccggc tccaacaccg
     4081 aaggtgccca cggccatgtc ctgaccgtcg gtggtcaggt agatcggcgt gacgatcgac
     4141 ttcaggtcgt ccgaccacgt gccgtactcc tgcaacccgt aggtaccgtt cacgtaggcg
     4201 agcgggtcgg acgtcgacag cggcacctgc aggtcccacg acagcgtgtt gtcgttggac
     4261 gttgtgaaga cgggggcgta gtccttgcca gtgaatgcaa ggagcatcgg cgtgtaatcc
     4321 ggggtcgagt ggtgaccgtt gataatgacg tagccgccgg catccaccca gtccttgatg
     4381 acctgcagag ccgcatcgct gacccatggc gtgccgccgg gttcgtagaa cgggttgttg
     4441 tcggagctct ccagctccgg gagcacgagg aggtcctgac ctgcgagcgc cgcgatccac
     4501 gcgtcggctg agccatcacc accgtcgaac ggtgtgaccg tgattccgag tccagtgaga
     4561 accgcggtct cgttctcgac ttcctggccg gagctggtgt cattcgcgag tccgttcgtg
     4621 aacaccacag cgttcacctc tgcgggggtt gccgggtagg cctgagcggc ggtcgacccc
     4681 gcgagcacca tcgccgaagc gagagcgagg gttgcgattg cggcgagcgc ggtcttcagg
     4741 ggcttcatgg gtactcctgg ctgtgcgggg taattgctga aaccctatcg gctacggcgc
     4801 tcccgcaaca atcgaaacac cgcacggagt caggccagaa gcgccgcgcc cacctcatca
     4861 aggccgatac ttgtgcgctc gccagtgcgg cgatcccaca gttcgacgcg gccttctgcg
     4921 gcatcccgac cgacgatgac gatctgggga acgccgagca gttcggcgtc accgaacttc
     4981 acgccgggcg aaaccttggg gcgatcgtcg aagagcacgt cgaagccact ctcctccagt
     5041 gagccgacga gcgcctcggc ggtctcgtag atgacctcct cgcgacccgc cgcgatcacg
     5101 tgcacgtcga acggcgcgat gttgcg
//
LOCUS       DAOSOU010000162         5121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_16432_length_5121_cov_5.273194, whole genome shotgun sequence.
ACCESSION   DAOSOU010000162 DAOSOU010000000
VERSION     DAOSOU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16432_length_5121_cov_5.273194"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLW15_08835"
     CDS             complement(<1..312)
                     /locus_tag="PLW15_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT34537.1"
                     /translation="MSDVVITQRGRLGHVLLNRPQAINALTHEMVAEIAAALAAWATD
                     DSVETVAISGAGERGLCAGGDVTAMYRSAVAEGADAGVEAAAFWADEYALNSTIAHYP
                     KP"
     gene            336..1265
                     /locus_tag="PLW15_08840"
     CDS             336..1265
                     /locus_tag="PLW15_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOT34538.1"
                     /translation="MTTSTRWERARASRWFALVWVVPALLVLALVVVLGARALRDLPG
                     VQAWLVEFPGTVPLPDDAPVGFPAWLNWQHFLNAFFLLFIFRTGWQLHQAKRPAAFWT
                     RTTGPFRRRNPPVRIGIPLWFHLSLDAVWVLNGVVYIVLLFATGQWVRLVPRTWDIVP
                     NAISAGLQYASLNWPTENGWVNYNALQLLAYFATVFIAAPLALATGIRLAPGLAARLR
                     PLDRVVPLKVTRLVHYVVLLYFVAFTIVHVTLVLATGALRNLNHMYAGRDDETWWGFG
                     IFALSIVVMAVGWMLARPTLLARVAELTGTVRR"
     assembly_gap    1446..1545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1546..2327
                     /locus_tag="PLW15_08845"
     CDS             <1546..2327
                     /locus_tag="PLW15_08845"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HOT34539.1"
                     /translation="PEVRNAFRPRTVDQLYSALDDARQNTKIGVVLITGNGPSPKDGG
                     WAFCSGGDQRIRGREGYEYHEGESKGLNRAGAARLHILEVQRLIRIMPKVVIAVVPGW
                     AAGGGHSLHVVCDLTIASAEHGKFKQTDADVGSFDGGYGSAYLARQVGQKVAREIFFL
                     AEEYSAQRAYEMGAINAVVPHAELEKTALKWAHTILRKSPTSIRMLKFSFNAIDDGLS
                     GQQVLMGEATRLAYGTDEAVEGRDSFLEKREPDWSPYPWQY"
     gene            2353..2760
                     /locus_tag="PLW15_08850"
     CDS             2353..2760
                     /locus_tag="PLW15_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34540.1"
                     /translation="MTRFGIDALTAIRIARESLAVPESHKLVAPNRLPSDAMSVLYRA
                     VREGNETAEEARRILDGITTMGIRLLGDRVSRATAWRVAEKLGWDDTTRAEYVAVAQL
                     QADAFVTLDPELAAAADGVVPLADFDALSQSAP"
     gene            complement(2757..3683)
                     /locus_tag="PLW15_08855"
     CDS             complement(2757..3683)
                     /locus_tag="PLW15_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT34541.1"
                     /translation="MSGLHIVIGAGGLGRSVAEHLVSGGERVRLVSRTADARVTGAES
                     VGADATNPSDLDVALAGASVVYQCAQPAYTRWAQEFPQLQTGILDAAVRAGADLVIAD
                     NLYMYGDPNGAVITEVSPENPATKKGRVRKAMADAAVQAHREGQLRVAISRPADYFGP
                     GHDQSSKAIFGNAARGRAMQFFGRMDAPHTLSYLPDAAAAMAALGTTEAGWGSVWIPP
                     VQPAVTQQELAERVWAAAGNSGRPKVRVTGQGAIRLVGLMVPVVREFAEMSYQYEKPF
                     VVDSSRFESTFGAAPTANDVAIAATVEHYRAS"
     gene            3748..4353
                     /locus_tag="PLW15_08860"
     CDS             3748..4353
                     /locus_tag="PLW15_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT34542.1"
                     /translation="MSTSPSRTGYHHGDLRNALLAAGSEVAREVGSEELSLREVARRA
                     GVSHTAAYNHFADKKDLLRGLAIVAFEQLAVELREAAERDDGLEELAVAYLRFAMAHP
                     AEFGFMFQRSLCMPDGVPDPLEEASRGSQAVLAERVIQLQAAGVLRAENPEIIGLALW
                     SQIHGITTIVLDTPVFKSIPAEQAEGLARACVDAIVVGLRA"
     gene            complement(4411..4617)
                     /locus_tag="PLW15_08865"
     CDS             complement(4411..4617)
                     /locus_tag="PLW15_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOT34543.1"
                     /translation="MEVGESLSFDAAVAMVVVGALLGGFIGSAVFGIGLEGFFSIQTW
                     LLAIGGAIVVLLIYGLATRGSRRA"
BASE COUNT          800 a         1741 c         1694 g          786 t
ORIGIN      
        1 gggcttgggg tagtgggcga tggtggagtt gagggcgtac tcgtcggccc agaacgcggc
       61 ggcctcgact ccggcgtcag caccctcggc gaccgccgag cggtacatcg cggtgacgtc
      121 gccgcccgca cacaggccgc gctcgccagc cccggagatg gcgacagtct cgaccgagtc
      181 atcggtcgcc caggcggcga gcgccgcggc gatctcggcc accatctcgt gggtgagcgc
      241 gttgatcgcc tgcgggcggt tcagcagcac gtgcccgagg cggccccgct gggtgatcac
      301 aacgtcggac acagccgcaa cactaggctg gtttcgtgac cacgtcgacc cgctgggagc
      361 gagcccgggc aagccgttgg ttcgcactgg tgtgggtcgt tccggcgctg ctggtgctcg
      421 ccctggtcgt ggttctcggc gcgagggccc tgcgcgatct gccgggcgtg caggcgtggc
      481 tggtcgaatt tccgggcacg gtgccgctac cggatgacgc gcccgtcggt ttccccgcct
      541 ggctgaactg gcagcacttc ctcaacgcct tcttcctgct gttcatcttc cgcaccggat
      601 ggcagctgca ccaggccaag cgcccggccg cgttctggac ccgcacgacc ggcccgttcc
      661 ggcgccgcaa tccgccggtg cgcatcggca tcccgctgtg gttccacctc agcctcgacg
      721 cggtctgggt gctcaacggc gtggtctaca tcgtgctgct gttcgcgacc ggccagtggg
      781 tgcggctcgt gccgcgcacc tgggacatcg tgcccaacgc gatctcggcc ggtctgcagt
      841 acgcgtccct gaactggccg accgagaacg gctgggtcaa ctacaacgcg ctgcagctgc
      901 tggcgtactt cgccacggtg ttcatcgcgg cgccgctggc tctggccacg ggcatccggt
      961 tggcgccggg tttggcagca cggctgcggc ccctcgaccg cgtcgtgccg ctgaaggtga
     1021 cgcggctcgt gcactacgtg gtgctgctct acttcgtggc gttcacgatc gtgcacgtca
     1081 cgctcgtgct cgcgaccggc gcgctgcgca acctcaacca catgtacgcg ggtcgcgacg
     1141 acgagacctg gtgggggttc ggcatcttcg cgctgtcgat cgtggtgatg gccgtcggct
     1201 ggatgctcgc ccggccgacc ttgctggccc gagtcgctga gctcacgggg accgtgcggc
     1261 ggtaggccac gtcattaggc tgggcacatg gcattcatct ccgacctgtt cgacgcggac
     1321 gcctggcgtg aggtgcacgg cttcgagtcg ttcgacgaca tcacgtacca ccacgacgtc
     1381 accggccgga tcgcccgcat cgccttcaac cggcccgagg ttcgtaacgc cttccgcccg
     1441 cgcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggccc gaggttcgta
     1561 acgccttccg cccgcgcacg gtcgaccagc tgtacagcgc gctcgacgac gcccgccaga
     1621 acaccaagat cggcgtcgtg ctcatcacgg gcaacgggcc cagccccaag gacggcgggt
     1681 gggccttctg ctcgggaggc gaccagcgca tccgcggtcg cgaaggctac gagtatcacg
     1741 agggcgagag caagggcctg aaccgggcgg gggctgcgcg gctgcacatc ctcgaggtgc
     1801 agcggctcat ccgcatcatg cccaaggtcg tcatcgcggt cgtacccggc tgggcggccg
     1861 gcggcgggca ctcgctgcac gtggtgtgcg acctcacgat cgccagcgcc gagcacggca
     1921 agttcaagca gacggatgcc gacgtcggct cgttcgacgg cggctacggc agcgcgtacc
     1981 tcgcgcgcca ggtcggccag aaggtcgcgc gcgagatctt cttcctcgcg gaggagtact
     2041 cggcgcagcg ggcctatgag atgggagcca tcaacgccgt ggttccccac gccgaactcg
     2101 agaagaccgc cctgaagtgg gcccacacca tcctccgcaa gtcgccgacg tcgatccgca
     2161 tgctcaagtt ctcgttcaac gcgatcgacg acggactctc cggccagcag gtgctcatgg
     2221 gcgaggcgac ccgcctggcc tacggaaccg acgaggccgt cgaggggcgc gactcgttcc
     2281 tcgagaagcg cgaacccgac tggtcgccct acccgtggca gtactgacgg tcgtcgaccc
     2341 gatactggag ccatgacccg cttcggcatc gacgccctga ccgcgatccg catcgcccgc
     2401 gagagtctcg cggtgcccga gtcgcacaag ctggtcgcgc cgaaccggtt gccgtccgac
     2461 gcgatgtccg tgctctaccg cgcggtgcgg gaggggaacg agaccgcgga agaggcccgc
     2521 cgcatcctcg acgggatcac gaccatgggc atccgtctgc tcggcgaccg ggtctcgcga
     2581 gcgacggcct ggcgcgttgc cgagaagctg gggtgggatg acacgacccg cgccgagtac
     2641 gtcgccgtcg cgcaactgca ggccgacgcc ttcgtgaccc tcgatcccga gctggcggcg
     2701 gctgccgacg gggtcgtgcc gctggccgac ttcgacgcgc tctcgcagtc cgcgccctag
     2761 ctcgcccggt agtgctcgac cgtcgccgcg atcgcgacgt cattggccgt tggcgccgca
     2821 ccgaacgtcg actcgaaccg ggacgagtcg acgacgaacg gcttctcgta ctggtagctc
     2881 atctccgcga actcccgcac gacgggcacc atcagaccga ccaaccgaat cgcaccctga
     2941 ccggtgacgc gcaccttcgg ccgaccggag ttgcccgcgg ccgcccacac gcgctcggcg
     3001 agctcctgct gcgtgaccgc gggctgcacc ggtgggatcc agaccgagcc ccacccggcc
     3061 tcggtcgtgc cgagcgcggc cattgccgct gcggcatcgg gcaggtagct cagcgtgtgc
     3121 ggcgcgtcca tacgcccgaa gaactgcatg gctcggccgc gcgcggcgtt gccgaagatc
     3181 gccttgctcg actggtcgtg ccccggcccg aagtagtcgg cgggacgcga gatcgccacg
     3241 cgcagttgac cctcgcggtg ggcttgcacc gcggcatccg ccatcgcctt gcgcacccgg
     3301 cccttcttgg tggccgggtt ctcgggcgac acctcggtga tcacggcgcc gttcgggtcg
     3361 ccgtacatgt acaggttgtc ggcgatgacg aggtcggcgc cggcccgcac ggccgcgtcg
     3421 aggatgccgg tctgcaactg cggaaactcc tgcgcccacc gcgtgtaggc cggctgcgcg
     3481 cactgataga cgacggatgc cccggcaagc gccacatcga ggtcggacgg gttcgtggca
     3541 tccgccccaa ccgactccgc tcccgtaact cgcgcatctg ctgttcggct caccagccgc
     3601 acccgctcac cacccgagac aaggtgttca gccacagaac ggccgaggcc gcccgcaccg
     3661 atgacgatat gcaatccact cacgacaaca ctcaactttc cactggaaac attatgacca
     3721 gtggcaattt atggcagaat gtaaccaatg tcaacttccc cttcgcgcac gggctaccac
     3781 cacggcgact tgcgcaatgc gctgctcgcc gcaggtagcg aggtcgcccg cgaagtcggg
     3841 tcggaagagc tgtcgctgcg cgaggtggcg cggcgcgcgg gtgtcagcca caccgccgcg
     3901 tacaaccact tcgccgacaa gaaggacctg ctgcgcgggc tcgcgatcgt ggcgttcgag
     3961 cagctcgctg tcgagcttcg agaggcggcc gaacgcgacg acggtctgga ggagctggcg
     4021 gtcgcctacc tccggttcgc catggctcac ccggcggagt tcgggttcat gttccagcgc
     4081 agtctctgca tgcccgacgg ggtgcccgac ccgctcgagg aggcctcgcg agggtcccag
     4141 gcggtgctag cggaacgggt catccagctc caggcggccg gcgtgctgcg tgccgagaac
     4201 ccggagatca tcggcctggc gctgtggagc cagatccacg gcatcaccac catcgtgctc
     4261 gacacacccg tgttcaagtc gattccagcc gagcaggcgg aaggcttagc gcgggcgtgc
     4321 gtcgacgcca tcgtcgtggg cctgcgcgcc tagccgcgac aacgaagaac ggggcgaccc
     4381 cgaagggccg ccccgttctc gtgggtggaa ctacgcgcga cgcgagcccc gcgtggcgag
     4441 gccgtagatc agcagcacga cgatggcgcc accaatagcc agcagccagg tctggatgga
     4501 gaagaacccc tccaggccga tgccgaacac ggcgctgccg atgaagccac cgaggagcgc
     4561 gccgacgacc accatggcca cggcggcatc gaacgagaga gactcgccga cctccatcgt
     4621 gtgcaggatg tcgtccccga gaagccccac gaacacgttg cctcgcttct gcacgaatcc
     4681 gatcgtgcgg ttcggggcga gcaagcagta ggtgtccggc gcgatgagcg agatgtcgag
     4741 cgagtcggtg acaaagcgcg acggcagcag tctcgtggtg gcgtcggcgg ttcggttcag
     4801 tgtcatcatg cactcctgtc tgttctgcaa tccaatcccg atcccgccgg atgccgtccc
     4861 ccattgacac aggcagtggg cgtgtggcat ggctcggacc ggtgggcgac tagatcgcag
     4921 ccggtaccgg atgtcaaccc ccaggcagtc tcggggcgga ggtggcagcg ttgaagaagc
     4981 acagccaccc tgtgcggaaa tggagtcaca tcatgggtat ggacgacaag atcaagaacg
     5041 ccgctgatga ggccgtgggc cgcgtcaagg agggcgtcgg tgccgcgatc aaggcgagcg
     5101 gcatcccgcg cgaggagctc t
//
LOCUS       DAOSOU010000163         5104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_16496_length_5104_cov_6.112696, whole genome shotgun sequence.
ACCESSION   DAOSOU010000163 DAOSOU010000000
VERSION     DAOSOU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16496_length_5104_cov_6.112696"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLW15_08870"
     CDS             <1..338
                     /locus_tag="PLW15_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34544.1"
                     /translation="CIDFPQQQQVVEIVDNRDGTLSLFTTVLDHAAPVSPGTGATTLD
                     LAAQSREFALNDWAESPTMRRGSPFDRNTELLLPAPFDLSRITDAALEAEHVAARARV
                     LAHESGLTS"
     gene            335..685
                     /locus_tag="PLW15_08875"
     CDS             335..685
                     /locus_tag="PLW15_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34545.1"
                     /translation="MRALIPVAALAVAVPLALTGCSQIAAIAPVGGAHVSEVRYAALD
                     ILVQKEVDVLTVPVCTTVERAVTCTGSTLDGAAIEVDSPADDPTNLTVTVDGAVLYSG
                     SIQDVLDAAVEPAP"
     gene            682..1290
                     /locus_tag="PLW15_08880"
     CDS             682..1290
                     /locus_tag="PLW15_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34546.1"
                     /translation="MSAWGRALGTSARVWVPVAAAVTAAQVLLVVGDPVPVASPGFVA
                     AALASGAVFAIGLWLVSRALTLAIAGEPVRWRSVLAAHPALVWAIVVVVLLVLLGLLS
                     PYLTVVGLWFGLLVMPAASAGASNPLPAAGHTIRRQPTRAILAAVVVAVVWALGALGM
                     LLLGFFVTGPLSAALTWIALTVLGTVVLAVWTDLYARARVTR"
     gene            complement(1280..2242)
                     /locus_tag="PLW15_08885"
     CDS             complement(1280..2242)
                     /locus_tag="PLW15_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOT34547.1"
                     /translation="MKAAMHDVYGDASVVTIRDAAVPEPAAGEVLIRVHAAGLDRGVW
                     HLLTGTPLMARLALGLRTPRQKMLGLDVAGVVERVGAGVTEFAPGDRVFGGGTGTFAE
                     FAIAKPKALAPMPAGLSFEQAAASPVSGLTALQALTKAGPVAGKSVLIIGAGSGVGSF
                     AIQLAKAQGAIVTAVVSPGKIDFVKRLGADHVIDYTVDDLGDGSHDVILDIAGIRPID
                     ELRGVLAPRGTVVFVGGEGGGPMLNGMQRQIGAGLFGPRGQKFVTLMGTTNTADLLTL
                     TQYLEAGTVVPAVEQRYPLEQAADAIRYIGQGHATGKTVITVSE"
     gene            complement(2245..2466)
                     /locus_tag="PLW15_08890"
     CDS             complement(2245..2466)
                     /locus_tag="PLW15_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT34548.1"
                     /translation="MVKPTRVTNSIRQLRFGAGEMTQADLADRIGVTRQTVIAIEQGR
                     YSPSLEMAFQLARVFGVGLDDVFQYPEEN"
     gene            complement(2460..2903)
                     /locus_tag="PLW15_08895"
     CDS             complement(2460..2903)
                     /locus_tag="PLW15_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34549.1"
                     /translation="MSFEERGVWILLTVAVAAYAVYLFLILGGPADVPLAERPYVVPM
                     LWTIGGAIVGGIVLSIFSGILTGIISRRGTVDKKDQRDKEINRLGEHIGSSFVVIGGV
                     SALILAWIEAPYFWIANVIYLGFVLSGIVASIAKLVAYRRGFQSW"
     gene            complement(3055..3127)
                     /locus_tag="PLW15_08900"
     tRNA            complement(3055..3127)
                     /locus_tag="PLW15_08900"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3092..3094),aa:Ala,seq:tgc)
     gene            complement(3303..3376)
                     /locus_tag="PLW15_08905"
     tRNA            complement(3303..3376)
                     /locus_tag="PLW15_08905"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3340..3342),aa:Ile,seq:gat)
     gene            complement(3464..3871)
                     /locus_tag="PLW15_08910"
     CDS             complement(3464..3871)
                     /locus_tag="PLW15_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HOT34550.1"
                     /translation="MSSVAEKLQRKSQRQVAVKQVRLKLVYIDFWSAVKLSFLVAVSL
                     GIVLVVATIFIWIVLNSTGIFGSLDNILKDILGDPEFSVAKQFSLGQVALFSFIVAIL
                     NIVVGTALGAIASMLYNFSVRLTGGLLVGFTNN"
     gene            complement(3864..>5104)
                     /locus_tag="PLW15_08915"
     CDS             complement(3864..>5104)
                     /locus_tag="PLW15_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOT34551.1"
                     /translation="LRRLAALERQKIIDEHDELERLIATYHHILATPSEQRTIIKDEL
                     TEIVDRFGDDRRTEVMFGFDGDMSVEDLIPEEEMVITVTRGGYIKRTRSDNYRSQHRG
                     GKGVKGAQLRADDVVNHFFVTTTHHWLLFFTNTGRVYRAKAYEIQEAGRDAKGQHVAN
                     LLAMQPGEEIAQILDIRDYKAAKYLVLATLGGLIKKTALTEYDTNRTGGIIAINLRED
                     DELVSAMLVEEDDDVLLVSRKGMSIRFTASDAALRPMGRSTSGVIGMHFREEDQLLSA
                     SVVGEDGYVFVVTEGGYAKRTSADQYRVQGRGGLGIKVAKLSDHRGDLAGALIVGDED
                     EVLVVLASGKVVRSSVAEVPAKGRDTMGVVFARFADDDKIIAIARNSERNLENQELDT
                     EADEAADEVESTGRDESVDE"
BASE COUNT          818 a         1696 c         1657 g          933 t
ORIGIN      
        1 cgtgcatcga cttcccgcag cagcaacagg tcgtcgagat cgtcgacaac cgcgacggca
       61 cgctgtcgct gttcacgacg gtgctcgatc acgcggcccc cgtgtcgccg ggcaccggcg
      121 cgaccaccct cgatctcgcc gctcagagcc gcgagttcgc cctcaacgac tgggcggagt
      181 cccccacgat gcgtcggggt tcgcccttcg accgcaacac cgagctgctg ttgcccgccc
      241 ccttcgatct gtcgagaatc acggatgccg cgctcgaagc cgaacacgtc gccgcgcgcg
      301 cccgggtgct cgcccacgag agcggactca cctcatgagg gcgttgatcc cggtcgccgc
      361 gctcgctgtc gcggtcccgc tcgccctcac cggatgcagc cagatcgcgg ccatcgcgcc
      421 cgtcggcgga gcgcacgtct cggaggtgcg ctacgcggcc ctggacatcc tcgtgcagaa
      481 ggaggtcgac gtgctcacgg ttcccgtctg cacgaccgtc gagcgtgcgg tcacctgcac
      541 gggatcgacg ctcgatggag ccgcgatcga ggtggactcc cccgccgatg acccgacgaa
      601 cctcaccgtg acagtcgacg gcgccgtgct gtactcgggg agcatccagg acgtgctgga
      661 tgccgcagtg gagcccgccc cgtgagcgcg tggggccgcg cgctcggcac cagcgctcgc
      721 gtgtgggttc cggtggctgc ggccgtgacc gctgctcagg tactgctggt cgtgggtgac
      781 ccggtgccgg tggcctcgcc cgggttcgtt gccgccgcgc tcgcgtccgg cgctgtgttc
      841 gcgatcgggc tctggctcgt gtcgcgggcg ctcacgctgg cgatcgccgg agagccggtg
      901 cgctggcggt cggtgctggc ggcgcatccg gctctcgtgt gggcgatcgt ggtcgtcgtg
      961 ctgctcgtgc tgttggggtt gctgagcccg tacctcaccg tggtgggact gtggttcggg
     1021 ctgctggtca tgcccgcagc gtcggccggg gcatccaacc ccctgcccgc cgcggggcac
     1081 accattcggc gccagccgac ccgggcgatc cttgccgcgg tggtcgtcgc cgtcgtctgg
     1141 gcgctcggcg ccctggggat gcttctgctc gggttcttcg tgaccggtcc gctgtctgct
     1201 gcgctcacct ggatcgcgct caccgtgctg ggcaccgtgg tgctggccgt gtggaccgac
     1261 ctctacgccc gcgcgcgcgt cactcgctga cggtgatcac ggtcttgccc gtggcatgcc
     1321 cctgcccgat gtagcgaatc gcatcggcgg cctgctcgag agggtagcgc tgttctacgg
     1381 ctgggaccac ggtgcccgct tcgagatact gagtcagggt caggaggtcg gccgtgttcg
     1441 tggtgcccat gagggtcacg aatttctggc cacgcgggcc gaagagtccg gcgccgatct
     1501 gacgctgcat gccgttgagc atcggtccac cgccctcgcc gccgacgaac acgaccgtgc
     1561 cgcgaggtgc gagcacgccg cgcaattcat cgatcgggcg gatgcccgcg atgtcgagaa
     1621 tcacgtcgtg actcccgtcg cccagatcgt cgaccgtgta gtcgatcacg tggtcggcac
     1681 ccaatcgctt cacgaagtcg atcttgcccg ggctgaccac cgcggtgacg atcgcgccct
     1741 gtgccttcgc gagctggatg gcgaacgaac cgacaccgct gcccgcaccg atgatgagca
     1801 ccgacttgcc agcgaccggg ccggccttcg tcagcgcctg gagcgctgtc agtcctgaga
     1861 ccggggatgc cgcggcctgc tcgaacgaga gtcctgcagg catcggggcg agagccttcg
     1921 gcttcgcgat cgcgaactcg gcgaaggtac ctgtgccacc gccgaagacg cggtcacccg
     1981 gcgcgaactc ggtcacccca gcacccaccc gttcgacgac accggccacg tcgagaccga
     2041 gcatcttctg gcggggcgtg cgcaggccca gggcgagcct cgccatgagc ggcgtgcccg
     2101 tgaggaggtg ccagacgccg cggtcgagcc cggcggcgtg caccctgatg agcacctcgc
     2161 ctgcggcggg ctcgggcacg gcggcatccc ggatcgtcac gacgctcgcg tcgccgtaga
     2221 catcgtgcat tgctgctttc atgatcagtt ctcctcgggg tactggaaga cgtcgtcgag
     2281 gccgaccccg aagactcgtg caagttggaa tgccatctcg agcgacggcg agtatctgcc
     2341 ctgctcgatg gcgataacgg tctggcgcgt aacgccgatc cggtcggcca gatcggcctg
     2401 ggtcatctcg ccggcaccga accgcagctg acggatcgag ttggtgaccc gggtcggctt
     2461 caccatgact ggaagccgcg acggtaggcg acgagcttgg cgatcgaggc gacaatgccg
     2521 gagagaacga agcccaggta gatcacgttc gcgatccaga agtacggggc ttcgatccac
     2581 gccagaatca gggcgctcac gccgccgatg acgacgaacg acgatccgat gtgctcgccc
     2641 aggcggttga tctccttgtc gcgctggtcc ttcttgtcca ccgtgccccg gcggctgatg
     2701 atgccggtca ggatgcccga gaagatgctc agcacgatgc cgccgacaat cgcgccaccg
     2761 atcgtccaca gcatgggcac gacgtagggg cgctcggcca gcggcacgtc ggccggcccg
     2821 ccgaggatga ggaacaggta tacggcgtag gcagcgacgg caaccgtcag cagaatccac
     2881 acgcctcttt cttcgaatga cacgatgctc tcctatgtaa agaaatgttg acatggatga
     2941 atgtacggtc tgttgtacat cgtgtcaagt tttctttact cacaggcgtg tcgtcgccac
     3001 gagctcccgc tggaacgaag agaggccccg ccaaacggcg gggcctctca gttgtggagc
     3061 ctaggggaat cgaacccctg acctcctgct tgcaaagcag gcgctctacc aattgagcta
     3121 aggccccgtg ctagctggtg cgccgccgct gctgcgcgct ggagcttagg gcgaggccat
     3181 ttctggcctc gctgcgacgc cagcgccgat cgccgtcccg gcgatcggta actaccaccg
     3241 ggacgatgac gcgagcgcca tcccaactaa acgggtgaag gaatcccttg tggagattcc
     3301 agtgggggta ccaggacttg aacctgggac ctcttcgtta tcagcgaagc gctctaaccg
     3361 cctgagctat acccccgaga ccagataaga acttacccca tccggttcgc tttgatgtaa
     3421 tcgtcgatct cgatgtgcga cttcgccgct actcgatcgc cgatcagttg ttcgtgaagc
     3481 ccaccagcag gccgccggtc agccgcacgc tgaagttgta cagcatgctc gcgatcgcgc
     3541 cgagcgcggt accgacgacg atgttgagga tcgcaacgat gaaggagaac agtgctacct
     3601 gacccagcga gaactgcttg gccacgctga actcgggatc accgaggatg tccttgagga
     3661 tgttgtcgag gctgccgaag atgcccgtcg agttcaacac gatccagatg aagatggtcg
     3721 cgacgaccag cacgatcccc agggacaccg ccacgaggaa cgacagcttc actgccgacc
     3781 agaagtcgat gtacacgagc ttgaggcgaa cctgcttgac cgccacctgg cgctgcgact
     3841 tgcgctgcag cttctctgcg acactactca tctactgact cgtcccttcc agtggactcg
     3901 acctcgtcgg cagcctcgtc ggcttccgtg tcgagttcct gattctctag attccgctca
     3961 ctgttcctgg cgatcgcaat gatcttgtcg tcgtcggcaa accgggcgaa aacaacaccc
     4021 atggtgtcgc ggcccttagc gggaacctca gcgacagacg agcgtaccac cttgccgctg
     4081 gcaagaacca caaggacctc gtcctcgtcg ccgacgatca gggcacccgc gaggtcgccg
     4141 cggtggtcgc tcagcttggc caccttgatg cccagaccgc cccgaccctg cacgcggtac
     4201 tggtccgccg aggtgcgctt cgcgtagccg ccttcggtga ccacgaacac gtatccgtct
     4261 tcgccgacga cgcttgcgct cagcagctgg tcctcctcgc ggaagtgcat gccgatgacg
     4321 ccggaggtgg atcgtcccat ggggcgcagt gcggcatccg atgcggtgaa cctgatcgac
     4381 atgcccttgc gcgagaccag cagcacgtcg tcgtcctcct cgacgagcat cgccgagacc
     4441 agctcgtcgt cttcgcgaag gttgatggcg atgatgccgc cggtgcggtt ggtgtcgtat
     4501 tcggtcagtg ccgtcttctt gatgagaccg cccagggtag cgagaacgag gtacttcgcg
     4561 gccttgtagt cgcggatgtc gaggatctgc gcgatctcct cccccggctg catcgcgagc
     4621 agattggcga cgtgctggcc cttcgcgtcg cggccggcct cctggatctc gtatgccttc
     4681 gcgcggtata cgcgtcccgt gttggtgaag aacaggagcc agtgatgggt ggtcgtcacg
     4741 aagaagtggt tgacgacgtc atccgcccgc aactgggcgc ccttgacacc cttgccgccg
     4801 cggtgctggc tgcggtagtt gtcgctgcgg gtgcgcttga tgtatccgcc gcgcgtgacc
     4861 gtgatcacca tctcctcttc ggggatgagg tcttccacgc tcatgtcgcc gtcgaagccg
     4921 aacatcacct cggtgcgacg gtcgtcgccg aagcgatcga cgatctcggt gagctcgtcc
     4981 ttgatgatgg tgcgctgctc gctcggggtg gcgaggatgt ggtggtacgt agcgatcagt
     5041 cgctcgagct cgtcgtgctc atcgatgatc ttctggcgct cgagcgcggc caggcggcgc
     5101 agct
//
LOCUS       DAOSOU010000164         4919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_17283_length_4919_cov_6.081620, whole genome shotgun sequence.
ACCESSION   DAOSOU010000164 DAOSOU010000000
VERSION     DAOSOU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17283_length_4919_cov_6.081620"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /gene="leuS"
                     /locus_tag="PLW15_08920"
     CDS             complement(<1..1431)
                     /gene="leuS"
                     /locus_tag="PLW15_08920"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT34552.1"
                     /translation="MSTPPETAVSNDQAAVGSDGDGPPPYEPTVIEPRWQQFWRDDAT
                     YEIENDDPRPHYYVLSMYPYPSGAAHMGHVRNYTFGDLITRYRTMGGHAVLSPMGFDS
                     FGLPAENAAIKTGVHPREFTDARIEELRSSLLRIGGGYDWRREVKSHDPEIIRWSQWI
                     FLKFYEAGLVYRRMAPVNWDPVDQTVLANEQVLPDGTADRSGAVVERIDLEQWFFKIT
                     DYAQQLLDDLEQVEWPQRVITQQRNWIGRSEGAEFDLAVVDADGNDTGESFRVFTTRP
                     DTSFGMTFCVLAPEHPLVATIVTDDRRADVEAFQATAKLTSEIDRLSTERALDKRGCF
                     TGAYARNPFTGKSVPVYLADYVLGTYGTGAIMAVPGGDQRDWDFATAYGLDIIRTTQP
                     PADFDGEAYAGEGPAINSEWLNGLSVADAKARAIEWLVEQGIGEAKVTFRLRDWLLSR
                     QRFWGCPIPFVYCDDCGLQPVPEDQLP"
     gene            1594..1666
                     /locus_tag="PLW15_08925"
     tRNA            1594..1666
                     /locus_tag="PLW15_08925"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1627..1629,aa:Ala,seq:ggc)
     gene            complement(2086..2853)
                     /locus_tag="PLW15_08930"
     CDS             complement(2086..2853)
                     /locus_tag="PLW15_08930"
                     /inference="COORDINATES: protein motif:HMM:NF020873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HOT34553.1"
                     /translation="MSDVRLPDGLVDGRHTPVFDATSLPERLEVVHRTTVWATLHVQS
                     GTVRYVDLVGPDRRDVRLVAGDSVVIVPGVDHQVEPSTDATFFVQFYRETTTPPIGEA
                     PRMSAGVERGSGPWPRPGGDLDSPEEIFELVTRQYADVVQDDLLEPYFTFGPGHLDWQ
                     AHIASVSDYWLHVVLLAPGYDIDVLESHRHLHEHQAFTPELFDRWLQIFVDTVDGGWS
                     GPNASLAKKKAIGMSRAMANRYLGKGAWKANLSESGS"
     gene            complement(2972..3682)
                     /gene="ric"
                     /locus_tag="PLW15_08935"
     CDS             complement(2972..3682)
                     /gene="ric"
                     /locus_tag="PLW15_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOT34554.1"
                     /translation="MNISTDHRLAEIVDLVPGAPRVLESFDLDYCCGGQRPLGDACDL
                     AGIDVAEVIEALAGLDDGPAPDWVPMSPALLVDHLEATHHAYLHAELPRIEALADKVV
                     GVHGARHPELVEVARLCTSLREDLEPHMAKEERVLFPMVRELASAVSPPSFHCGSVAN
                     PISMMMREHEQTGSILEALRSQTDGYHAPDDACASYRALYEALDALEADTHLHIHKEN
                     NVLFPAVLELEKHWEAAR"
     gene            complement(3679..3891)
                     /locus_tag="PLW15_08940"
     CDS             complement(3679..3891)
                     /locus_tag="PLW15_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT34555.1"
                     /translation="MRVRTHPALCTGFGNCHRWAPHVYPLDADGRVDIHLLDVPAELA
                     AEARLGAQVCPQLAITIIEPTPRSAT"
     gene            complement(3927..4334)
                     /locus_tag="PLW15_08945"
     CDS             complement(3927..4334)
                     /locus_tag="PLW15_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT34556.1"
                     /translation="MRLEITRRSDLATRALIELARLGRRTKSSELADAIGTTPGFLSQ
                     AMTPLAARGWVRSEPGPTGGYALIADLADVSVLEVIEAIEGPSDAGRCVLEDRPCGDA
                     GPCALHQPWQRARTQLLDELSNSPLAGLPKGRK"
BASE COUNT          759 a         1623 c         1700 g          837 t
ORIGIN      
        1 cggcaactgg tcttcgggca cgggctgcag gccgcagtcg tcgcagtaga cgaacgggat
       61 gggacaaccc cagaaccgct gacggctcag cagccaatcg cgcagacgga acgtgacctt
      121 ggcctcgccg atgccctgct ccaccaacca ttcgatcgcc cgagccttgg cgtcggcgac
      181 ggagagcccg ttcagccact cgctgttgat cgccggaccc tcgcccgcgt acgcctcgcc
      241 gtcgaagtcg gccggcggct gtgtcgtgcg gatgatgtcg aggccgtagg ccgtcgcgaa
      301 atcccagtcg cgctggtcac cgcctggcac cgccatgatg gcgccggtgc cgtaggtgcc
      361 cagcacgtag tcggccaggt acacggggac ggacttcccg gtgaacgggt tgcgggcgta
      421 ggcgccggtg aagcaaccgc gcttgtcgag cgcgcgttcg gtcgacaagc ggtcgatctc
      481 gctcgtgagc ttggccgttg cctggaacgc ctcgacgtcc gcacgacggt cgtcggtgac
      541 gatcgtcgcg accaacgggt gctcgggcgc cagcacgcag aacgtcatgc cgaagctggt
      601 gtcggggcgc gtggtgaaca cgcggaacga ctcgccggtg tcgttgccgt cggcgtcgac
      661 cacggccagg tcgaactcgg ccccctcgga acggccgatc cagttgcgct gctgggtgat
      721 gacgcgctgc ggccactcga cctgctccag gtcgtcgagc agctgctgcg cgtagtcggt
      781 gatcttgaag aaccactgtt cgagatcgat gcgctcgacg accgcgcccg agcggtcggc
      841 cgtgccatcg ggcagcacct gctcgttggc cagcaccgtc tggtcgaccg gatcccagtt
      901 caccggggcc atgcggcggt acaccaaacc ggcttcgtag aacttcagga agatccactg
      961 cgaccacctg atgatctcgg ggtcgtggct cttgacctcg cgtcgccagt cgtagccgcc
     1021 gccgatccgc aggagcgagc tgcgcagctc ctcgatgcgg gcgtcggtga actcacgggg
     1081 atgcacaccg gtcttgatcg ctgcgttctc ggcgggcagg ccgaagctgt cgaaacccat
     1141 cgggctgagg accgcgtggc cgcccatggt gcggtaccgc gtgatgaggt cgccgaacgt
     1201 gtagttgcga acgtggccca tgtgcgccgc acccgaggga taggggtaca tcgacagcac
     1261 gtagtagtgc gggcgcgggt cgtcgttctc gatctcgtac gtggcgtcgt cccgccagaa
     1321 ctgctgccag cgcggctcga tgacggtggg ttcgtacgga ggaggaccgt cgccgtcgga
     1381 accgacggcg gcttggtcat tcgagacggc agtctcgggt ggggtcgaca tgtcgcccag
     1441 cgtagtgagg gggatgcgac agcccgaaac gagaaaccgc cctgccggtc cgaccaactc
     1501 gagtcccacc cgggccgatc gtgccggctg ggcctcaagt tcgcccgagg ttcgtggcgc
     1561 gcatcgcgtg ctgctatcgt ggctgcgtcc cgcggggctg tagctcaatt gggagagcgc
     1621 ttccatggca tggaagaggt agtcggttcg atcccgatca gctccacaac ggaatcccgc
     1681 aagaacactg aggtccggcc agagcgccgg accttgttcg ttgcggcgaa ggccggacgg
     1741 gtgtttcgtt cacctgtcgc gctcatcgcc tgtcgtgccg aggagcttcc ccatcggccc
     1801 ggcggcgacc gtgaccgact cgctcagcgg tcctcaccgg acgatgacgg ccgaaatccg
     1861 ggtgcgccga ccgggcggac ctcggcgaac gcgtccgggc cagcgcggtc gcgggatgct
     1921 tcgcgggatg gagcgaacgg tcgagctcgc gtgtgatctg gccgtaccgg caccttcccg
     1981 ccgccactcg gcccaggtgt cggtgcattc gtcccgagct cgtgacctac cgcaccggac
     2041 ggcctgcccg acgagtcacc ggatacgaca aggccgctgc gccgatcacg agcccgactc
     2101 gctcaggttc gccttccagg cacctttgcc gaggtacctg ttcgccatgg cccgcgacat
     2161 gccgatggcc ttcttcttgg cgagcgaggc gttgggaccg ctccacccgc catcgacggt
     2221 gtcgacgaag atctgcaacc aacgatcgaa caactccggc gtgaacgcct ggtgttcgtg
     2281 gaggtgacgg tgactctcca ggacatcgat gtcgtagccg ggcgccaaca ggacgacatg
     2341 gagccagtag tcactgacgg atgcgatgtg ggcctgccag tcgaggtggc ccggcccgaa
     2401 cgtgaagtac ggctccagca ggtcgtcctg cacgacatcc gcgtactgcc gggtcaccag
     2461 ctcgaagatc tcctcggggg aatccaggtc acctcccggt cgaggccagg ggccactccc
     2521 acgctcgacg cccgctgaca tacgaggcgc ttcgccgatc ggaggagtgg tggtctcccg
     2581 gtagaactga acgaagaacg tcgcgtcggt cgacggctcg acctgatggt cgactcccgg
     2641 cacaatgacg acactgtcgc cggccaccag gcgcacgtcc cgtcgatcag ggcccaccag
     2701 atcgacgtag cggacggttc cggattggac gtgcagcgtc gcccacaccg tcgtgcgatg
     2761 cacgacttcg aggcgttctg gcaggctggt cgcatcgaag accggggtgt gccggccgtc
     2821 gaccaagccg tcgggaagtc gcacatcgct cacgtgctcg acctcacact tcgcatggca
     2881 tccacttccc tccacgggcg ggttgccaag gtccagagca acgacgtcat cgcaggcgct
     2941 cgcagcgccg gaactccggg gtccaggcgc ctcaacgcgc tgcctcccag tgcttctcca
     3001 gttcgagaac ggcagggaac agcacgttgt tctccttgtg gatgtggagg tgcgtgtcgg
     3061 cctcgagcgc gtcgagcgcc tcgtacaggg cccggtagct ggcgcaggca tcgtcggggg
     3121 cgtggtagcc gtcggtctgt gagcgcagtg cttcgaggat ggatccggtc tgctcgtgtt
     3181 cgcgcatcat catcgagatc gggttcgcca ccgatccgca gtggaacgat ggcggcgaga
     3241 ccgcggaggc cagctcccgg accatcggga acaacacccg ctcttccttc gccatgtgtg
     3301 gctcgagatc ctcgcggagc gaggtgcaca atcttgcgac ctccacgagt tcgggatgcc
     3361 gcgctccgtg tacgccgacc accttgtcgg ccagggcctc gatgcgtggc aactcggcgt
     3421 ggaggtacgc gtggtgggtc gcctccaggt ggtcgacgag caacgccggg ctcatgggca
     3481 cccagtcggg cgcggggccg tcgtcgagcc cggcgagtgc ctcgatgacc tcggcgacgt
     3541 cgatgccggc gaggtcgcaa gcatcaccga ggggacgctg gccaccgcag cagtagtcga
     3601 gatcgaagga ttcgagcacc cgtggtgctc cgggcacgag atcgacgatc tcggcgagtc
     3661 ggtggtcggt cgagatgttc atgttgcgct cctcggggtc ggttcgatga tcgtgatcgc
     3721 gagctgcggg cagacctgag cgccgaggcg ggcctcggcg gcgagttcgg cggggacgtc
     3781 gagcaggtgg atgtcgacgc ggccgtcggc gtcgagcggg tacacgtgag gggcccatcg
     3841 gtgacagttc ccgaagccgg tgcacagggc aggatgggtt cgtacgcgca cgccggtctc
     3901 ggctgccgtc gacgtctcgt ccgcgatcac tttcggccct ttggcagccc tgccagcggc
     3961 gagttcgaca gctcgtcgag cagctgggtg cgggcccgtt gccagggttg gtggagggcg
     4021 cacgggccgg cgtcgccgca gggccggtcc tcgagcacgc accgtccggc gtcggatggg
     4081 ccctcgatgg cttcaatgac ctcgagcacg ctgacgtcgg cgaggtcggc gatgagcgcg
     4141 tagccaccgg tgggaccggg ctccgaacgc acccagcccc tcgccgccag gggcgtcatc
     4201 gcttgggaga ggaagcccgg cgtcgtgccg atcgcgtcgg ccagctcgct cgacttcgtg
     4261 cggcgtccga gacgtgcgag ctcgatgagt gcgcgggtgg cgagatcgga tcggcgggtg
     4321 atttctaatc tcatagtcgt ggactataca atgtcgggat ggctcgacaa ccgcaccgac
     4381 gacgaagcca cccgaccgag acggatcgga ccgaaacgtc tcctcgcccg acccacccgt
     4441 ggccgcaatc ggtcgccctg gtgaccggcg tcgtggtgct cctcgtcgtc ctctccttgc
     4501 tgagcacctt ccgatgaccc ggaccacggc gtcgacgacg gaggaccggg catcgggagc
     4561 accgtcgccg cgccaccgct ccacgttgcg ttctgttgcg ttgccgacgg agcacggcgg
     4621 ctggggcctc acgctggaat cagcggtgct cggtcttgcg gtgagatggt ccgccgccgg
     4681 agtgtgcctc gctgtcgctg cgttcctggc gttcctcgcc cgcacaccac tgaaggtggt
     4741 gctggtggat ctccgccggc accgggtcct ccctcggacc cgactggctc gcctcgtcct
     4801 cgctgctgag atcgtcgcaa tggcgtcgct catcgcagcg gccgtcacac tcggtgcctc
     4861 cgccttctgg gcgccggtgg tactcatcgg accgctcgcc accgtcgagc tgtggttcg
//
LOCUS       DAOSOU010000165         4915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_17317_length_4915_cov_6.686214, whole genome shotgun sequence.
ACCESSION   DAOSOU010000165 DAOSOU010000000
VERSION     DAOSOU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17317_length_4915_cov_6.686214"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLW15_08950"
     CDS             <1..847
                     /locus_tag="PLW15_08950"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34557.1"
                     /translation="GPLVGAGNSLLETTGQISRIGGPGLAGLLLTILTAPVLLIADAI
                     SFLGSAFALSRVRDTETLRPAAERESLRREIAEGLRFVFRHPVLWRITLTTGTSNLFG
                     MLVGTLEAILILRELELSPASFGLIMGIGSVGGLLGALATPWVTKHVGEGTAVSLSAL
                     AMAAASAAIPLAGTFPGVALPILILGSFVNSFLVLVYNITQVTMRQRLTPARLLGRMN
                     ASIRFVVWGVMPIGALAAGALGSTIGILPTMWIGVVGTAVGSFWVLFSPITRMRTLPT
                     ELVEQ"
     gene            complement(1092..1847)
                     /gene="aqpZ"
                     /locus_tag="PLW15_08955"
     CDS             complement(1092..1847)
                     /gene="aqpZ"
                     /locus_tag="PLW15_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HOT34558.1"
                     /translation="MAKTVANADVDLDTTSSLPQRVTAELFGTFLLVSGVVGTALFAG
                     NSVGILGIALAVGLTVVAGAYAVGHISGGHFNPAVTIGAAAAGRFAWSDVGYYVFAQI
                     IGGALGTTFIFVLAANGPAGLLDQVMGSGFAANGFAEHSPGGFGLVAVLVTEVVLTAV
                     FLYVILGVTDRRAASGFAPLAIGLTLTLIHLVAIPVSGASVNPARSIASAIYGGPDAL
                     IQLWVFIVAPIVGALIAGLTYGRLFDRKATKAE"
     gene            2007..3665
                     /locus_tag="PLW15_08960"
     CDS             2007..3665
                     /locus_tag="PLW15_08960"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOT34559.1"
                     /translation="MDATTTQSGAPVASDSESLTVGPDGATVLHDRYLLEKLAQFHRE
                     RIPERVVHAKGGGAHGEFVVTGDVSEYTSAAVFQPGAVTPTLQRFSSVAGEQGSPDTW
                     RDVRGFSVRFYTTEGNYDIVGNNTPVFFIRDAMKFPDFIHSQKRMPGSGLRDADMQWD
                     FWTLSPESAHQVLYLMGDRGLPRSWREMNGFGSHTFQWTAESGERSWVKYHFVTNQGD
                     HTLDAAEAELIAGADADHYRRDLHESIARGDFPSWDLKVQVMPFETAKTYRFNPFDVT
                     KVWPHADFPLIPVGTHTLNRNPENFFAEIEQAAFSPGNTVRGVGISPDKMLMARVFAY
                     PDSQRYRVGANFAEVPVNTPHVDTHSYSQDGASRARFAPASSPVYAPNSFGGPVAAPE
                     PGLDSRWESDGELMRAAASLHSEDSDFGQAGTLYRDVLTNDSRDRLHVTLRGQRSLLT
                     VPGIVERFDAYWTSVDADLGAAVSGQSGAGQSQPGQGEPGQSQAGQNQPGQGEPGQSE
                     PGHNQSGHQSGHNQPGQNQPGQNQPGQSRAGEPGQSRPAALSAM"
     gene            complement(3632..4909)
                     /locus_tag="PLW15_08965"
     CDS             complement(3632..4909)
                     /locus_tag="PLW15_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT34560.1"
                     /translation="MAFGVVPIAVSGDPAMFLYWLAFCLVVGFIDLAIAASPRAVRLE
                     RDLPSRMRLGETVQSTLLVTNTGRRGMHGLIRDGWPPSAGATPRRTRLAVPAGERRAI
                     TTTLTPFRRGERRAAHVTIRSFGPLRLWARQATLSVPGAVLVLPPFNSRKHLPSRLAR
                     LRELDGATSVMVRGQGTEFDSLRDYVRGDDVRSIDWRATARRAAASGPGLVVRTWRPE
                     RDRRVVIIVDSGRTSAARIDNEPRIDTAFESALLLGALASSAGDRVDLVIFDRRVRAR
                     VQGATGAELLSRMVSSMAPVEPELIEMDWSAVPSLVRSVTPHRALVVLATTIDAPGAS
                     RGLLSVLPQLTRRHTVVVSSVTDPTVLDATHSRTTRDEVYLAAAAERAVLDQERVAAA
                     IRQLGGDVVTGAPQELPPALADRYIALKAAGRL"
BASE COUNT          756 a         1688 c         1772 g          699 t
ORIGIN      
        1 ggggccgctc gtgggcgccg gcaactcgct gctggagacc acgggccaga tcagtcgcat
       61 cggcggcccc ggcctggccg gcctgctact gaccatcctc accgcgccag tgctgctcat
      121 cgccgacgcg atctccttcc tcggctcggc gttcgcgctc tcccgagtgc gtgacaccga
      181 gacgttgcgc ccggccgctg agcgagagag ccttcgtcgc gagatcgccg aggggctgcg
      241 gttcgtgttc cgccacccgg tgctgtggcg catcaccctc accaccggca catccaacct
      301 gttcgggatg ctcgtcggca ccctcgaggc catcctcatc ctgcgcgaac tcgagttgtc
      361 gcccgcaagc ttcggcctca tcatggggat cggctccgtc ggcggcctgc tcggggcgct
      421 cgctaccccg tgggtgacca agcacgtggg ggagggcacc gcggtgtcgc tgtccgccct
      481 cgcgatggct gcggcatccg ctgcgatacc gctcgccgga acgttccccg gtgtcgccct
      541 gccgatactc atcctcgggt cgttcgtgaa ctcgttcctc gtgctggtct acaacatcac
      601 acaggtgacc atgaggcagc ggctgacgcc cgcgcgcctg ctggggcgta tgaacgcatc
      661 cattcgcttc gtcgtctggg gcgtcatgcc gatcggcgcg ctcgcggctg gggcgctcgg
      721 gtcaacgatc ggcatcctgc cgaccatgtg gatcggcgtc gtagggaccg cggtcggctc
      781 gttctgggtg ctgttctcgc cgatcacgcg catgcgcact ctgccgaccg agctcgtgga
      841 gcagtagcgc gctcgtcgga cggtgtcgcc ctcggcgcgc acgctcaggt cgcagcagcc
      901 gtggcgccgg ccgcgctcgg tgccgtgcgc ggatgcacgg atgcggcacg gcacgccgcg
      961 gcgggtgcga cctgagcggc gcgccgcccg cgatccgtgc atcgcggccc gtgctgctgt
     1021 cacgccgcag accgcgcctt aacgcacgga tgcccggggc cgaccgaccc cgggcatcct
     1081 gagcactgcg gctactcggc cttggtggcc ttgcggtcga agaggcggcc gtaggtgagg
     1141 ccggcgatca gggcgcccac gatgggggcg acgatgaaca cccagagctg gatgagcgca
     1201 tcgggcccac cgtagatggc ggacgcgatc gagcgggccg ggttcaccga cgcgcccgag
     1261 accgggatcg cgaccaggtg gatgagggtc agcgtgaggc cgatcgccag cggcgcgaag
     1321 ccggacgccg cgcgacggtc ggtcacgccg aggatcacgt agaggaacac ggcggtcagc
     1381 acgacctcgg tgacgagcac ggcgacgagt ccgaagccgc cgggggagtg ctcggcgaag
     1441 ccgttagcgg cgaatccgga gcccatgacc tggtcgagga ggccggcggg gccgttggcg
     1501 gcgagcacga agatgaaggt ggtgccgaga gctcctccga tgatctgcgc gaacacgtag
     1561 tagccgacat ccgaccaggc gaaccggccg gcggccgcgg cgccgatggt gacggcgggg
     1621 ttgaagtggc cgcccgagat gtggccgacg gcgtaggcgc cggcgacaac ggtcaggccc
     1681 acggcgagtg cgatgccgag gatgcccacg gagttgcccg cgaagagtgc cgtgccgacg
     1741 actccggaga cgagcaggaa ggtgccgaac aactcggccg tcactcgctg cggcagcgag
     1801 gaggtggtgt cgagatcgac atcggcattg gcgacggtct tggccacgat ggagcctttc
     1861 tgttgagttc gctgggaacg actcgaccct atcgctcctg acccgcgccg ggtgggccgc
     1921 cgcgtcgcga gtctgtgagg tcggtcaggt agggggagca cgatcggccc atcgccttcc
     1981 ttcgggcgcg agcgaaggtc gaatccatgg atgccacgac tacccagtcg ggagcgccgg
     2041 tagccagcga ctccgagtcc ctcaccgtcg ggccggacgg cgcaacggtc ctccacgacc
     2101 gctatctgct ggagaagctc gcgcagttcc accgggagcg catcccggag cgggtcgtgc
     2161 acgccaaggg cggcggcgcg cacggcgagt tcgtggtcac cggcgacgtg tccgagtaca
     2221 cgtcggcggc agtgttccag ccgggcgcag tcacgccgac gctgcagcgc ttctcgagcg
     2281 tcgccggcga gcaggggtcg cccgacacct ggcgcgacgt gcgcggcttc tcggtgcgct
     2341 tctacacgac cgagggcaac tacgacatcg tgggcaacaa caccccggtg ttcttcatcc
     2401 gcgacgcgat gaagttcccc gacttcatcc actcccagaa gcggatgccc ggctcgggtc
     2461 tgcgcgacgc cgacatgcag tgggacttct ggaccctctc gcccgagtcg gctcaccagg
     2521 tgctctacct catgggcgac cggggtctgc cgcggtcgtg gcgcgagatg aacggcttcg
     2581 gctcgcacac cttccagtgg accgccgagt ccggcgagcg ttcgtgggtg aagtaccact
     2641 tcgtcaccaa ccagggcgat cacactctgg atgccgccga agccgagctc atcgccggtg
     2701 ccgatgccga tcactaccgc cgcgacctcc acgagtcgat cgcccgcggg gacttcccgt
     2761 cgtgggatct gaaggtgcag gtcatgccgt tcgagaccgc gaagacctac cggttcaatc
     2821 cgttcgacgt gacgaaggtc tggccgcacg ccgacttccc gctcatcccg gtcggcactc
     2881 acaccctgaa ccgcaatccg gagaacttct tcgccgagat cgagcaggcc gccttctcgc
     2941 ccggcaacac cgtgcgcggg gtcggcatca gcccggacaa gatgctcatg gcccgtgtgt
     3001 tcgcctatcc cgactcccag aggtaccggg tcggcgccaa cttcgccgag gtgccggtca
     3061 atacgccgca cgtcgacacg cacagctaca gccaggatgg cgcctcccgg gcgcgtttcg
     3121 ccccggcgag ctcgcccgtc tacgcgccga actcgttcgg cggtcctgtc gcggcaccgg
     3181 agccgggcct cgactcgcgc tgggagtccg atggtgagct gatgcgcgct gcggcgagcc
     3241 tgcactcgga ggattccgac ttcggtcagg cgggcacgct ctatcgtgac gtgctcacca
     3301 acgactcgcg cgaccggctg cacgtgaccc tgcgcggtca gcggagtctg ctcacggtgc
     3361 ccggcatcgt cgagcgattc gacgcctatt ggacgtcggt ggatgccgat ctcggcgccg
     3421 ccgtgagtgg tcagagtgga gcgggtcaga gccagccggg tcagggcgag ccgggtcaga
     3481 gccaggcggg tcagaaccag ccgggtcagg gcgagccggg tcagagtgag ccgggtcaca
     3541 accagtcggg ccaccagtcg ggccacaacc agccgggcca gaaccagccg ggccagaacc
     3601 agccgggcca gagccgggcg ggcgagccgg gtcagagccg gccggccgcc ttgagcgcga
     3661 tgtagcgatc cgcgagggcg ggcggcagct cctggggagc gccggtcacg acgtccccgc
     3721 cgagctgacg gatggcggcg gccacccgct cctggtcgag gacggcgcgc tccgcggcgg
     3781 cggccaggta cacctcgtcg cgcgtggtgc gggagtgcgt ggcatccaga accgtggggt
     3841 cggtgacgga tgacacgacc acagtgtgtc ggcgcgtcag ctggggcagc accgacagca
     3901 ggccgcggga cgcaccgggc gcgtcgatcg tggtggcgag caccacgagc gcccggtgcg
     3961 gggtcaccga gcgcacgagg gacggcaccg ccgaccagtc catctcgatg agttcgggtt
     4021 cgaccggagc catgctcgac accatgcgcg agagcagctc tgcgccggtt gccccctgca
     4081 cccgcgcccg aacccggcgg tcgaagatca cgaggtcgac gcggtcaccc gcgctggagg
     4141 cgagagctcc gagcagcagg gccgactcga acgccgtgtc gatgcgcggc tcgttgtcga
     4201 tgcgggccgc cgaggtgcgc ccgctgtcga cgatgatgac gacgcgtcgg tcgcgctcgg
     4261 gtcgccaggt gcgcacgacg agacccggcc cggatgccgc ggcgcgccga gcggtcgccc
     4321 tccagtcgat cgagcgcacg tcgtcgccgc gcacgtagtc gcgcagactg tcgaactcgg
     4381 tgccctggcc gcgcaccatg acgctcgtcg cgccgtcgag ctcgcgcagc cgcgccaggc
     4441 gggaaggcag gtgcttgcgt gagttgaacg gcggcagcac gagcaccgcg ccgggtaccg
     4501 acagcgtcgc ctgccgcgcc cagagtcgaa gcgggccgaa cgaccggatg gtcacatgag
     4561 ctgcgcgacg ctcgcctcga cggaacggcg tgagcgtcgt cgtgatcgcg cggcgctcgc
     4621 cggcgggaac ggccaggcgg gtgcgccgcg gggttgcgcc ggccgagggg ggccagccgt
     4681 cgcggatgag gccgtgcatc ccgcgcctgc cggtgttggt gacgagcagg gttgactgca
     4741 ccgtctcgcc gaggcgcatg cgcgacggca ggtcgcgctc gagtcgcacc gcgcgcggag
     4801 acgcagcgat ggcgaggtcg atgaacccga caacgaggca gaacgccagc cagtagagga
     4861 acatcgcggg gtcgcccgag acggcgatcg gcacaacgcc gaacgccacc agcag
//
LOCUS       DAOSOU010000166         4808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_17738_length_4808_cov_5.414054, whole genome shotgun sequence.
ACCESSION   DAOSOU010000166 DAOSOU010000000
VERSION     DAOSOU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17738_length_4808_cov_5.414054"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLW15_08970"
     CDS             <1..476
                     /locus_tag="PLW15_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205203.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT34561.1"
                     /translation="VPKAIVQERYERLVALQDRISWEENQKLVGQRLNVLVTAAEGRK
                     SSETHRLSGRAEDSRLVHFDVPVGSTVPRPGDVVSVTVTQAAPFHLIADSPAGVPLEV
                     RRTRAGDAWDRAEAESCAVPTPASQTASEPGRVSLGLPGLRVATAPIYDLADDLR"
     gene            490..1404
                     /gene="miaA"
                     /locus_tag="PLW15_08975"
     CDS             490..1404
                     /gene="miaA"
                     /locus_tag="PLW15_08975"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT34562.1"
                     /translation="MTLVVIVGATGTGKSDLSLSVAEGLSARGMPAEIVNADAMQLYR
                     GMDVGTAKLTIGERRGIPHHMLDVLDPCDEASVADYQRDARWAIEDIEARGSVPILVG
                     GSGLYVSSVIQDFRFPGTDPVVRARLEAELIETGPGTMYRRLLEVDPDAARAIGASNG
                     RRIVRALEVVEITGEPFGSGLPEDGPPWRPTVIIGLQTPREVLVDRLTARAAGMWDAG
                     LLDEVRGLRERELGVTASRAIGYAQAFAQLNGELTEAEAIEQTAALTRRYARRQVGWF
                     RRYGTARWLDSDDPARHATALALAMGED"
     gene            1408..2283
                     /gene="dapF"
                     /locus_tag="PLW15_08980"
     CDS             1408..2283
                     /gene="dapF"
                     /locus_tag="PLW15_08980"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT34563.1"
                     /translation="MATLEFTKGHGTGNDFVLFADPDGEIDLSAAQIAAICDRHFGVG
                     ADGVLRAVRSRSIPEGAAVLAEDPAAEWFMDYHNADGSVAEMCGNGIRVFSRFLVDNG
                     LAELEPGGTLPIGTRNGVRDVQRNSSGYQADLGRWELAGGEPLVRARELPVARPGLGI
                     SVGNPHVVVALASDEELDGTDLSYIPVIEPEPADGANVEFVVAADPLVKDGVGHIRMR
                     VHERGSGETLSCGTGAVAAALAIRHWAGAGAPNHWRVDVPGGTVGVRMFPAEDGEHVS
                     LSGPAELVYVGALEV"
     gene            complement(2298..2945)
                     /locus_tag="PLW15_08985"
     CDS             complement(2298..2945)
                     /locus_tag="PLW15_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT34564.1"
                     /translation="MASEHYFSSSPGSELKLRTIHAKILGRDYELSTANGVFSPDRID
                     TGTQVLLGSVPAPPPGGNLLDLGCGWGPIALTLALESPHATVWAVDVNERALDLVRTN
                     AAKLSITNVNAVLPDEVPPSIMFTTIWSNPPIRVGKNELHGLLQRWLPRLESGSDAWL
                     VVQRNLGSDSLHRWLQGAFPEEFSFLRASTNKGYRVLRARRRTGLTSPIDIIREQ"
     assembly_gap    3095..3194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3195..4235
                     /gene="hflX"
                     /locus_tag="PLW15_08990"
     CDS             <3195..4235
                     /gene="hflX"
                     /locus_tag="PLW15_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT34565.1"
                     /translation="VVADTELAPSQRRALEDIVGVKVIDRTAVILDIFSQHATSREGR
                     AQVELAQLEYLLPRLRGWGESMSRQAGGQVGAGNGMGSRGPGETKIELDRRRIHTRMS
                     RLRKQIKGFAPAREAKRANRNRFEVPSVAIAGYTNAGKSSLLNRITRAGVLVENALFA
                     TLDATVRRSATPDGRLFTLSDTVGFVRNLPHQLVEAFRSTLEEVGQSDVIIHVVDASH
                     PDPAAQLATVRDVIGEVGARDIPEIVAFNKSDLADDDQRLLLRGMEPTGIFVSARTGE
                     GIDELLARVAELLPAPEIEMTLLVPYDRGEIISRLHVQGRVLSTDYREDGTLVTAMVH
                     PARVEELKPFVA"
     gene            4360..>4808
                     /locus_tag="PLW15_08995"
     CDS             4360..>4808
                     /locus_tag="PLW15_08995"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOT34566.1"
                     /translation="MSGDLPCRSDATFSFELYPPRSPAAEANLFAAIDRLSALGPEFM
                     SVTYRATGHDRAASLAVLDKLIREHRVSAMAHLTCVGSSFDEASRVIREFLDAGVTSF
                     LALRGDPPEGFEEGDDFLGDFRSASELVQLIHNVQAERSPFRLGEVPG"
BASE COUNT          772 a         1578 c         1628 g          730 t
ORIGIN      
        1 aggtgcccaa ggcgatcgtg caggaacggt acgagcgact cgtcgccctg caggatcgca
       61 tctcgtggga ggagaaccag aagctcgtcg gtcagcggct gaatgtgctg gtcacggccg
      121 ccgaggggcg caagagctcc gagacccatc ggctgtccgg tcgggccgaa gacagtcgcc
      181 tcgtgcactt cgatgtgccc gtgggtagca cggtaccgcg ccccggcgac gtggtgagcg
      241 tgaccgtcac gcaggcggcg cccttccatc tgatcgccga ctcgcccgcc ggcgtgcccc
      301 tcgaggtgcg ccgcactcgc gcgggagacg cgtgggaccg cgcggaggcg gagtcctgcg
      361 ctgtgcccac gcccgcgtcg cagacggcgt cggagcccgg ccgggtgtca ctgggcctgc
      421 ccggcctcag agtcgccacg gcgccgatct acgatctcgc cgacgacctg cgctgagacg
      481 acggcagcca tgaccctcgt cgtgatcgtc ggggcgaccg gcactggtaa gtctgacctg
      541 tccctgagtg tcgctgaggg cctgtcggcc cgcgggatgc ccgcggagat cgtcaacgcc
      601 gacgcgatgc agctgtaccg cggcatggat gtgggaacgg cgaagctcac gatcggtgag
      661 cgccgtggca tcccgcacca catgctcgac gtgctcgacc cgtgcgacga agcgagcgtc
      721 gccgactatc agcgggatgc ccgctgggcg atcgaggaca tcgaggcccg cggcagcgtc
      781 ccgatcctcg tgggcggcag cggtctctac gtctcgagtg tcatccagga cttccgcttc
      841 cccggcacgg acccggtggt gcgcgcccgt ctcgaggcgg aactgatcga gaccggtccg
      901 gggacgatgt accgtcgtct gctcgaggtc gaccccgatg ccgctcgcgc gatcggcgca
      961 tccaacggtc ggcggatcgt gcgggcgctc gaggtcgtcg agatcacggg ggagcccttc
     1021 gggtccggtc tgcccgaaga cgggccgccg tggcgaccca ccgtcatcat cggcctgcag
     1081 accccgcggg aggtgctcgt cgatcgcttg acggcgcgag ccgccggcat gtgggacgcc
     1141 ggtcttctcg acgaggtcag gggtcttcgc gagcgggagc tcggggtgac ggccagccgc
     1201 gccatcgggt acgcccaggc attcgcacaa ctgaatggcg agttgaccga agcggaggca
     1261 atcgagcaga cggccgcact gacgagacgg tacgcccgcc gtcaggtcgg ctggttccgg
     1321 cgctacggca cagcccgctg gctcgactcc gacgatcccg ctcgccacgc gaccgcactt
     1381 gctcttgcga tgggggagga ttgacgcatg gcaacgctcg aattcaccaa gggtcacgga
     1441 accggcaacg acttcgtcct gttcgccgac cctgacggtg agatcgacct gagtgcagcc
     1501 cagatcgccg cgatctgcga ccgtcacttc ggtgtggggg ccgacggcgt gctgcgcgcc
     1561 gtgcgttcgc ggagcattcc cgagggcgcg gcggtgctcg ccgaagaccc ggccgccgag
     1621 tggttcatgg actatcacaa cgccgacggt tcggtcgccg agatgtgcgg caacggaatc
     1681 cgggtgttct cgcgcttcct ggtcgacaat ggcctcgccg agctggaacc cggcggcacc
     1741 ctgccgatcg gaacccgcaa cggggtgcgc gacgtgcagc gcaattcgtc gggttatcag
     1801 gccgacctgg gccggtggga actcgcgggc ggtgagcctc tcgttcgggc tcgtgagctg
     1861 ccggtcgcgc gaccgggtct cggaatctcg gtaggcaacc cccacgtggt cgttgcgctg
     1921 gccagcgacg aggagctcga cggcaccgac ctcagctaca tcccggtgat cgagcccgaa
     1981 ccggccgatg gcgccaacgt cgagttcgtc gtcgctgctg acccgctcgt caaggacggc
     2041 gtcggtcaca tccgcatgcg cgtgcacgag cgcggcagcg gagagaccct cagctgcggc
     2101 accggggccg tggccgcggc gctggcgatc aggcactggg ccggcgcggg tgcccccaac
     2161 cactggcggg tcgacgtgcc gggcggaacc gtcggggtgc gcatgttccc cgccgaggac
     2221 ggcgagcacg tctcgctgag cggccccgcc gaactggtct acgtcggcgc cctcgaggtc
     2281 tgactcctcg gtagcggcta ctgctcgcgg atgatgtcga tcggcgaggt cagtccggtg
     2341 cgccgccgcg cgcgcagcac ccgatagccc ttgttggtgg atgcccgcag aaaactgaac
     2401 tcctcgggaa acgcgccctg aagccaccgg tggagcgagt cggagccgag gttgcgctgc
     2461 acgacgagcc aggcatccga gccgctctcc agcctcggca gccagcgctg gaggagaccg
     2521 tggagttcgt tcttgccgac ccggatcgga gggttggacc agatcgtggt gaacatgatc
     2581 gacgggggca cctcgtcagg cagaactgcg ttaacattag ttatggacaa tttcgcggcg
     2641 ttcgtgcgca ccagatccag tgcgcgctcg ttgacgtcca ccgcccacac cgtcgcatga
     2701 ggtgactcga gcgcgagcgt gagggcgatc ggaccccagc cgcaaccgag atcaagcagg
     2761 ttgccacccg gtggtggcgc cggaacacta cccaggagca cctgggttcc ggtgtcgatg
     2821 cgatccgggc tgaacacacc attggccgtc gacaactcgt agtcccgccc cagaatcttg
     2881 gcgtggatcg tgcggagttt gagctcactt ccgggtgacg acgagaagta gtgctcactg
     2941 gccatgcaga gaacttaccg aagactgact agcaagggtt aatagcaatg accgaatctc
     3001 accacgacga cacatcaccc gagaccatga caccggatgc cgcggccgca cgcagcgacg
     3061 tcgatcgcgt cttggccacc gagggcgccc gttcnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnngtcgtc gccgacaccg agctggcacc cagccagcgg cgcgcgctcg
     3241 aggacatcgt cggcgtcaag gtcatcgacc gcacggcggt gatcctcgac atcttcagcc
     3301 agcacgcgac cagtcgcgag ggtcgggctc aggtcgagct cgcccagctc gagtacctcc
     3361 tgccgcgact gcgcgggtgg ggtgagtcga tgtcccgcca ggccggagga caggtcggcg
     3421 ccggcaacgg aatgggaagc cgcggaccgg gtgagacgaa gatcgagctc gaccgtcgcc
     3481 gcatccacac ccgcatgtcg aggctgcgca agcagatcaa gggcttcgcg ccggcgcgcg
     3541 aggcgaagcg ggccaaccgc aatcgcttcg aggtgccgag cgtcgcgatc gccggctaca
     3601 cgaacgcggg caagtcgagc ctgctcaacc gcatcacgcg ggcgggcgtg ctcgtcgaga
     3661 acgcgctgtt cgcgacactg gatgccacgg tgcgccgttc ggcgacgccc gacggtcgcc
     3721 tcttcaccct ctccgacacc gtcgggttcg tgcgcaacct gccgcatcaa ctcgtggagg
     3781 ccttccggtc gacgctcgag gaggtgggac agtccgacgt catcatccat gtggtggacg
     3841 cctctcaccc ggacccggcg gcacagctgg cgacggtacg agacgtcatc ggtgaggtcg
     3901 gggctcgcga catcccggag atcgtggctt tcaacaagag cgatctcgct gatgacgacc
     3961 agaggcttct gctgcggggg atggagccga ccggcatctt cgtgtcggcc cgcacgggtg
     4021 agggcatcga cgagctcctg gcgcgggtgg ccgaactgct gccggccccc gagatcgaga
     4081 tgacgctgct cgtgccctac gacaggggcg agatcatctc gcgcctgcac gtgcagggcc
     4141 gggtgctctc gaccgactac cgcgaagatg gcaccctggt gaccgccatg gttcatcccg
     4201 cgcgtgtgga ggagctgaag ccgttcgtgg cgtaacacgc gttaacccga gtgggcggga
     4261 cgcctagaat tgacgggctg gggtgaccac cccacaggag cgacagccga gcccggcacc
     4321 cgcccactcg gaatgcacca ttaccgaagg gcccgtgcgg tgtctggaga tctcccgtgt
     4381 cgaagtgacg cgaccttctc ctttgagctc tatccacccc ggtcacctgc ggccgaagcc
     4441 aacctcttcg cggcgatcga ccggctcagc gctctcggtc ccgaattcat gtcagtgacc
     4501 taccgggcca caggccacga tcgcgccgcg tctctcgcgg tgctcgacaa gctgatccgg
     4561 gagcacaggg tgagcgccat ggcgcatctc acctgcgtgg gctcgtcgtt cgatgaggcg
     4621 agccgggtca tccgggaatt cctcgacgcg ggagtgacca gcttcctcgc ccttcgcggt
     4681 gatccacccg aggggttcga ggagggcgac gacttcctcg gcgacttccg cagcgccagc
     4741 gagctggtgc agctgatcca caatgtgcag gccgagcgca gcccgttccg gctcggcgag
     4801 gtgcccgg
//
LOCUS       DAOSOU010000167         4802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_17765_length_4802_cov_3.810196, whole genome shotgun sequence.
ACCESSION   DAOSOU010000167 DAOSOU010000000
VERSION     DAOSOU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17765_length_4802_cov_3.810196"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLW15_09000"
     CDS             complement(<1..1092)
                     /locus_tag="PLW15_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT34567.1"
                     /translation="MPGWEATTLLLLAAYVVLGFVATVLISTNRRPSAAVAWVLVIIF
                     IPFLGALWYLLVGAGRLPRARREKQREVSDLILSRTEGLDAVSHGGEWPAWLASAARM
                     NLTLGALPIVGGNRAELLADYEGSIAAMAAEIDTATEYVHVEFYILVLDDTTRVFFDA
                     LRRARDRGVLVRVLVDHLSSIMFPNRRETFGALDEMGAEWHAMLPLRPLRGQWQRPDL
                     RNHRKLVVVDGRVGFTGSQNLIDSTYLKKKNVARGLHWHELMVRLEGPVVRELNAVFV
                     TDWYSESGVLLPLDTSPVVLGTDDALLDAQVLPSGPSFDNDNNLKLFGYLLQSAQRRV
                     SITSPYFVPDESTLMAIVTAAARGLDVELF"
     gene            complement(1092..2156)
                     /locus_tag="PLW15_09005"
     CDS             complement(1092..2156)
                     /locus_tag="PLW15_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT34568.1"
                     /translation="MSEMPGYRLLNSGPFRFGFITTLGVLLALVLGLAIGSLTYALTL
                     IFFALFVSLGLYPLVLKLESLKFSRTGAVLTVMGIFIVVVGLLLWRIIPNIVEQAGQL
                     IKYIPAGLDDIEHQEWFLALNETFGGGLLPFVQTLQQALSDPAVWIAIGGGALHVSVS
                     IVNGTFGAIFVVALTLYFVASLETMKHGLYSLVPASRRAGFSEIAEEIFQSVGKYLSG
                     MFLLALINAAFSFVLLTILGVPWAGILAAFAIVITFIPVVGSLISASIMTVVSLFTSP
                     TTGLIVGIVMFVYLQLEAYVLTPRIVGKAIRIPGSLVLIGAMVGATLLGLLGALVACP
                     VSASILLITKKVIVPTQNER"
     gene            complement(2153..3664)
                     /locus_tag="PLW15_09010"
     CDS             complement(2153..3664)
                     /locus_tag="PLW15_09010"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT34569.1"
                     /translation="MAIDSRLIPADFTPVPSLDHAPKIIVSSTLPDDVEVVGFAVGSD
                     GKVPSALGTTFDALNRAGFAGTPGQTLVLPRTEGPVFIAVGVGSDPAQTATSLRDAAA
                     AFARAAARYSALALDLGEAFALEPSVVGQMVTEGTLLARYRFTELKPTTTDIPLASLT
                     IVSEPGTIEQVTAGAATGEVTSRAANLARDLANNPPGHLTATRMGTVGLDIADDYGLK
                     VEVFDKKQLIELGCGGLLGVNAGSAEEPRMIKMVYTPEGTPTSHVALVGKGIMYDAGG
                     ISLKPSDSMHLLMKMDMAGAAAIMGAMTALKALGVTSKVSAWLMCTDNMPSGTAMGLG
                     DVLTMRGGKTVEIKNTDAEGRLVMADALVLATEEHPDAIVDVATLTGSALQTLGTRVA
                     AVFGNDQALVDQAIAASQSTDEPIWQLPLEHRYRPQLNSAYADMSNMGGPFNVAITAA
                     LFLNEFVDGIPWAHLDIAGTMQSDTDDSWRTAGATGFATRLLIELLTNFTPTK"
     gene            complement(3675..>4802)
                     /locus_tag="PLW15_09015"
     CDS             complement(3675..>4802)
                     /locus_tag="PLW15_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT34570.1"
                     /translation="LVLTGSPVNVLISEFLVEAGEKPIGFFQIGLVGLPLLIGTVLIT
                     VLLGPKLLPRLTPKYVTKDLSMHAQTLALQYSIEDEQKLLTREAGLTEVVIPPRSELV
                     GQDVFPGMVTDSGDLVVVAIQRGGENLGKATLQVGDVMLLRGSWGALDANIAADPNVI
                     MVDSPAAIRRQAVPMGAKAKTALVILAGMVILLATGLVTPAVAALIAATCMVIFRVLS
                     VHQAHRSISWTTLILVGGMIPLSTAIQVSGTADLIANGIVATLGSASPYLLALGLALV
                     TVVLGQLISNTATALILAPIAIAIASETGVSPVPLLMSVAIAAAASFLTPVATPANTM
                     VFGPGGYRFGDYWKLGLPLALLFVAVATFLVPVFWPFYPTP"
BASE COUNT          874 a         1485 c         1678 g          765 t
ORIGIN      
        1 gaagagctcg acgtcgaggc cgcgcgcggc ggccgtgacg atcgccatga gcgtggactc
       61 gtcaggcacg aagtagggac tcgtgatgct caccctgcgc tgggcgctct gcagcagata
      121 gccgaacagc ttcagattgt tgtcgttgtc gaagctcggt ccgctcggga gcacctgcgc
      181 atccaggagt gcgtcgtctg tgccgagaac caccgggctc gtgtccaggg gcagcagcac
      241 ccccgactcg ctgtaccagt cggtcacgaa caccgcattg agctcccgca cgacgggacc
      301 ctcgagccgc accatcaact cgtgccagtg caggccgcgc gcgacgttct tcttcttgag
      361 atatgtcgag tcgatgaggt tctgcgagcc ggtgaatccg acccgcccat ccaccacgac
      421 gagcttgcgg tgattgcgca ggtccggtcg ctgccactga cctcgtaggg ggcgcagcgg
      481 cagcatcgcg tgccactcgg cacccatctc gtcgagggcg ccgaaggtct cgcgccgatt
      541 cgggaacatg atgctcgaca ggtgatcgac cagcacccgc accagcacgc cgcgatcgcg
      601 cgcgcggcgc agggcgtcga agaacacgcg ggtcgtgtca tccagaacga ggatgtagaa
      661 ctcgacgtgc acgtactcgg tcgccgtgtc gatctcggcg gccatcgccg cgatcgagcc
      721 ctcgtagtcg gccaggagct cggcacggtt gccgccgacg ataggcagcg cgcccagggt
      781 cagattcatg cgtgcagcac tcgcaagcca ggccggccat tcgcctccat ggctcacagc
      841 atcgagaccc tcggtgcgcg acaggatgag gtcgctgacc tcgcgctgct tctcccgccg
      901 ggcgcggggg agccgtccag caccgaccag caggtaccag agcgcgccga ggaacggaat
      961 gaagatgatc acgagcaccc aggcgacggc cgccgagggt cgccggttgg tcgagatcag
     1021 caccgtggcg acgaagccga gcacgacgta tgctgcgaga agcagcaggg tggtggcctc
     1081 ccagccgggc actagcgctc gttctgggtg ggaacgatga ccttcttggt gatcagcaga
     1141 atcgatgccg agacggggca ggcgacgagt gcgccgagca ggccgagaag ggtggcgccc
     1201 accatcgcgc cgatcagcac cagcgaaccg gggatgcgaa tcgccttacc gacgatccgc
     1261 ggcgttagca cgtaggcctc gagctgcagg tacacgaaca tcacgattcc cacgatgagt
     1321 cctgtcgtgg gcgaggtgaa caacgagacg accgtcatga tggaggcgct gatcaggctt
     1381 cccaccaccg ggatgaacgt gatcacgatc gcgaacgccg cgaggatgcc cgcccacggt
     1441 acgccgagga tggtgagcag cacgaacgag aacgccgcat tgatgagggc gagcaggaac
     1501 atcccgctca ggtacttgcc gaccgattgg aagatctcct cggcgatctc ggagaagcca
     1561 gcgcgccggg aggcgggcac cagcgagtac agcccgtgct tcatggtttc cagcgatgcc
     1621 acgaagtaga gcgtgagggc gaccacgaag attgccccga acgttccgtt gacgatcgac
     1681 acgctcacgt gcagggcgcc gccgccgatc gcgatccaga cggccgggtc ggagagcgcc
     1741 tgctgcagag tctggacgaa cgggaggagt ccgccgccga acgtctcgtt gagcgcgagg
     1801 aaccactcct gatgctcgat gtcgtcgagg ccagcgggaa tgtacttgat cagctgcccg
     1861 gcctgctcga cgatgttcgg gatgatgcgc cagagcagca gtccgaccac gacgatgaag
     1921 atgcccatga cggtcagcac ggccccggtg cgcgagaact tgagcgactc gagcttcagc
     1981 accagcggat agagccccag actgacgaac agggcgaaga agatgagcgt caacgcgtac
     2041 gtcagagacc cgatcgcgag accgagtacg agcgccagga gcacacccag cgtcgtgatg
     2101 aatccgaacc ggaacggccc cgagttcagt aggcggtatc cgggcatctc actcatttcg
     2161 tgggggtgaa gttcgtgagc agttcgatca gcagtcgggt cgcgaatccg gtcgcgcccg
     2221 ctgtgcgcca ggagtcgtcg gtgtcgctct gcatggtgcc cgcgatgtcg aggtgcgccc
     2281 acgggatgcc gtcgacgaac tcattgagga acagtgccgc agtgatcgcc acattgaacg
     2341 gaccgcccat gtttgacatg tccgcgtatg ccgagttcag ctgtgggcgg taccggtgct
     2401 cgagcggtag ctgccagatc ggctcgtcgg tcgactgaga ggccgcgatc gcctggtcga
     2461 cgagtgcctg atcgttgccg aacacggcgg cgacacgagt gccgagcgtc tggagggcgg
     2521 agccggtgag agtggcaacg tcgacgatgg catcgggatg ctcctccgtc gcgagcacga
     2581 gcgcgtcggc catcaccagg cgcccctcgg cgtcggtgtt cttgatctcg acggtcttgc
     2641 cgccgcgcat cgtcagcacg tcgccgagtc ccatggcagt tcccgagggc atgttgtcgg
     2701 tgcacatgag ccacgcgctg accttgctgg tcacgccgag cgccttcagc gcagtcatgg
     2761 cgcccatgat ggccgcagca ccggccatgt ccatcttcat gagcagatgc atggagtcgc
     2821 tcggcttgag ggagatgccg ccggcgtcgt acatgattcc cttgccgacg agggcgacgt
     2881 gcgatgtcgg ggtgccctcg ggggtgtaga ccatcttgat catgcgcggc tcctccgcgc
     2941 tgccggcgtt gaccccgagt aggcctccgc agcccagctc gatgagctgc ttcttgtcga
     3001 acacctcgac cttgaggccg tagtcgtcgg cgatgtcgag cccgacagtt cccatgcgcg
     3061 tggcggtgag gtggccggga gggttgttcg ccaagtcgcg agcgagattc gccgccctcg
     3121 aggtgacctc gcccgtcgct gcaccggccg tgacctgctc gatggttccg ggttcgctga
     3181 cgatcgtcag cgacgcgagc gggatgtctg tggtcgtcgg cttgagttcg gtgaaccggt
     3241 accgggccag cagcgtccct tccgtgacca tctggccgac cacgctcggt tccagggcga
     3301 atgcctcacc caggtcgaga gcaagtgcgg agtaccgtgc cgcggcgcgc gcgaatgcgg
     3361 cggcagcatc ccggaggctg gtagcggtct gcgcggggtc gctgcccaca ccgacggcga
     3421 tgaagaccgg accctcggtg cgcggcagga cgagcgtctg cccgggggtg ccggcgaacc
     3481 cggctcggtt cagggcgtcg aaggtggtgc cgagggccga gggcaccttt ccgtcggatc
     3541 cgacggcgaa gccgacgacc tcgacgtcgt cggggagggt gctcgagacg atgatcttcg
     3601 gtgcatgatc cagcgagggc accggggtga agtccgcggg gatcaggcga gagtcgatgg
     3661 ccatggggtt ctccttaggg ggtggggtag aaaggccaga agaccggcac gaggaaggtt
     3721 gctacggcga cgaacagcag ggccaatggc agaccgagct tccagtagtc gccgaagcgg
     3781 tagccacccg gtccgaagac catggtgttg gcgggagtcg cgaccggggt gaggaacgag
     3841 gccgccgcgg cgatggcaac cgacattaac agcggtaccg gagagacccc ggtttctgag
     3901 gcaatcgcga tggcgatcgg tgccaggatc agcgcggtcg cggtgttgct gatcagctgg
     3961 ccgagaacga ctgtcacgag cgcgagtccg agagccagca gataggggct ggccgacccg
     4021 agggtcgcga ctatgccgtt ggcgatcaga tccgcggtgc cgctgacctg gatcgccgtc
     4081 gagagcggga tcatgcctcc gacgaggatc agggttgtcc aggagatgga ccggtgggcc
     4141 tggtgcacgc tcagcacccg gaagatcacc atgcaggtcg cggcgatcag ggcggcgacg
     4201 gccggtgtca cgagaccggt cgcgaggagg atcaccattc cggcgaggat caccagcgcc
     4261 gtcttggcct tggcgcccat cggcactgcc tgacgacgga tggcggcggg cgagtccacc
     4321 atgatgacgt tcggatccgc cgcgatgttg gcgtccaggg cgccccaact tccgcggagc
     4381 agcatgacgt cgccgacctg gagggtcgcc tttcccaggt tctcgccacc gcgctggatc
     4441 gccacgacca cgaggtcgcc gctgtcggtg accattccgg ggaagacgtc ctggccgacg
     4501 agttcagagc gcgggggaat gacgacctcg gtcaggccgg cctcacgggt gaggagcttc
     4561 tgctcgtcct cgatggagta ctgcagcgcc agggtttggg cgtgcatgct gaggtctttg
     4621 gtcacgtact tcggcgtcag tcgaggcagc agcttcgggc cgaggagaac tgtgatgagc
     4681 accgtgccga tgagcagcgg caggccgacg agcccgatct ggaagaaccc gatcggcttc
     4741 tcacccgcct cgacgaggaa ctcggagatc agcacgttga cggggctacc ggtgagtacc
     4801 ag
//
LOCUS       DAOSOU010000168         4773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_17889_length_4773_cov_6.256889, whole genome shotgun sequence.
ACCESSION   DAOSOU010000168 DAOSOU010000000
VERSION     DAOSOU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17889_length_4773_cov_6.256889"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..553)
                     /locus_tag="PLW15_09020"
     CDS             complement(122..553)
                     /locus_tag="PLW15_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HOT34571.1"
                     /translation="METLFAVLHVVAAVFIIGPMAILPMAGLRSLRGGNSAQVNGFAR
                     ATNVFGWLSLVVAVLGFGLVAFVDPEDKLTYTTPWLLTSIILYAAAVTIVLSGVAPLM
                     KRAGERLADGKLAMEYRPIAALSGLSALLLVAVVVLMVWRP"
     gene            complement(668..2215)
                     /locus_tag="PLW15_09025"
     CDS             complement(668..2215)
                     /locus_tag="PLW15_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT34572.1"
                     /translation="MAEKDGTKVSGVTYTKAGDGYFEKRSLKRSAGVWGLWGLAVAAV
                     ISGDFSGWNFGIDFAGFGGMLIAFVILVVMYYGMIFSIGEMAAAQPHTGGAYSFARSA
                     MGPWGGLVTGLAETIEYVATTGVIVFFSAQYANGITTELLGFDLSANMWIWWIVLYVV
                     FIALNSAGANISFKFAIVVSIISIAILLVFSAMALFSGLFSWDSLWNIPPDEGQSTFL
                     PHGVLPILFALPFAMWFFLGIEELPLAAEESMNPTRDIPKAGLWARGTLIVTGLLVLF
                     LNTGVLGAAATGVSLEPLLDGFRAIVGDQLAAVLALFALIGLLASLQGIMFAYGRNMY
                     SLSRAGYYPKFFSLTGKRQTPWVALVVGAVIGFIALVVLDLLSKADAAGAGAVAGAII
                     LNIAVWGAVLAYLLQMVSFVILRKKFPNAKRPYKSPWGIPGAVVAAIISALIFLGFLL
                     NAAFQPAIIAIAIVYALILLGFALYGRHRLVLSPEEEYALSGGMHGDPETEGYDAMEG
                     EVFGDKK"
     gene            2396..3778
                     /locus_tag="PLW15_09030"
     CDS             2396..3778
                     /locus_tag="PLW15_09030"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOT34573.1"
                     /translation="MAHASTQTKTGNLTVQELTVMVAAKEIDTVIIAFTDMQGRLVGK
                     RASARLFLEELAEHGAECCNYLLAVDVEMNTVSGYAISSWERGYGDMAMMPDIDTLRL
                     VPWLPGTALVTADLTWLDGTPVAPDPRQVLKRQIDRLAKKGLSSFVGTELEFIVFEDS
                     YRDAWKKKYVDLYPASDYNIDYALLASSRMEPLLRDIRNGMDGAGMYCEGVKGECNLG
                     QQEIAFRYTDALGTCDNHSVYKNGAKEIADQHGKSLTFMAKYNEREGNSCHIHLSVVG
                     ENGEPVMADKDRPYGFSKLMEHWIAGQLATLRELSLFFAPNINSYKRFVEGSFAPTAV
                     AWGLDNRTCALRVVGHGRSLRVENRVPGGDVNQYLAVAALIAGGLYGIEHELELEPIF
                     EGNAYVSDAPRVPSSLREAAALFQASEVATEAFGEDVVAHYLNNARIEQAAYDAAITD
                     WERVRGFERF"
     gene            3915..4616
                     /locus_tag="PLW15_09035"
     CDS             3915..4616
                     /locus_tag="PLW15_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT34574.1"
                     /translation="MPASFLPKVYFEAVTRAGGIAVLLPPQPVDAEIADAVLDGLDGL
                     IITGGKDVEPARYGQEPHPTTDEPRRDRDAWEDALLTAAIDRGLPFLGICRGAQVLNV
                     ALGGTLHQHLPDVIGTTRYNLGQGRFAVNEVGVEASTKLGQLLQGDETVDVKSYHHQA
                     IDEVAPGLVVSARSDDGTIQAVELADHPFGVAVQWHPEEDAAEDARLFAGLVDAAKEY
                     RSVVERVEAEGAHHG"
BASE COUNT          931 a         1559 c         1570 g          713 t
ORIGIN      
        1 acacccgagg cgtttcgcgc acgcacctcg atcacccccg cgaacggcac tacggcaaga
       61 gggatcacca cgagcgcacc gagcgtgaaa tggatgaact cgagtgcgag cctcatggtc
      121 tctaggggcg ccagaccatg agtacgacta ctgccaccag caggagagcc gacagtcccg
      181 acaacgccgc gatcgggcgg tactccatcg cgagcttgcc gtcggcgagg cgctcacccg
      241 ctcgcttcat cagcggggcg actcccgaga gcacgatggt gacggcggcc gcgtagagga
      301 tgatcgaggt caacagccag ggcgtggtgt aggtgagctt gtcctccgga tcgacgaagg
      361 cgaccaagcc gaatccgagc accgcgacaa ccagtgacag ccacccgaag acgttggtgg
      421 cccgggcgaa gccgttcacc tgcgcggagt tgccaccgcg aagcgaacgg agacctgcca
      481 tgggcaggat ggccatcggg ccgatgatga agacggccgc aaccacgtgc agcacagcga
      541 agagggtttc catgacccca agagtgccgg ttcgacgccc cacgagacag gggcgaaggt
      601 cccatcgaac accgcagaac ggccccgcct cccgcaggag acgaggccgt tctgatcagc
      661 agcgaagcta cttcttgtcg ccgaagactt cgccctccat ggcgtcgtag ccctcggtct
      721 ccgggtcgcc gtgcatgccg cccgacagcg cgtactcctc ctcaggggag agcaccaagc
      781 ggtggcgacc gtagagggcg aaaccgagca ggatcagcgc gtagacgatc gcgatcgcga
      841 tgatcgccgg ctggaacgcc gcgttcagca ggaagcccag gaagatgagc gccgagatga
      901 tcgcggcgac aacggcaccg ggaatgcccc acgggctctt gtagggccgc ttggcgttcg
      961 ggaacttctt gcgcaggatc acgaacgaga ccatctggag caggtacgcg agcacggcac
     1021 cccagacggc gatgttcagg atgatcgcac cggcgaccgc accagcaccc gcggcatccg
     1081 ccttgctcag caggtcgagc acgacgaggg cgatgaagcc gatcaccgcg cccacgacca
     1141 gcgcgaccca gggggtctga cgcttgccgg tgagcgagaa gaacttcggg tagtacccgg
     1201 cgcgcgacag cgagtacatg ttgcggccgt aggcgaacat gatgccctgc agcgaggcga
     1261 gcagaccgat gagggcgaat agcgcgagca ctgcggcgag ctggtcgccg acaatggcgc
     1321 ggaatccgtc gagcagaggc tcgagcgaaa cacctgttgc tgcagctccg agcacgcccg
     1381 tgttgaggaa cagaacgaga agaccggtga cgatcagcgt tccgcgggcc cagaggccgg
     1441 ccttcgggat gtcgcgggtc gggttcatcg actcctcggc cgccagcgga agctcctcga
     1501 tgccgaggaa gaaccacatg gcgaacggca gggcgaacag gatcggcagc acaccgtgcg
     1561 gcaggaaggt gctctggccc tcatccggcg ggatgttcca gaggctgtcc cagctgaaca
     1621 ggccagagaa cagtgccatg gccgagaaga ccagaaggat tgcgatggaa atgatcgaca
     1681 cgacaatggc gaacttgaac gagatgttcg ctcccgccga gttgagcgcg atgaacacga
     1741 cgtagagcac gatccaccag atccacatgt tcgcggagag gtcgaagccg agcagttcgg
     1801 tcgtgatgcc attggcatac tgcgccgaga agaagacgat cacgcccgtc gttgcgacgt
     1861 actcgatcgt ctcggcgagc ccagtgacca gaccgcccca ggggcccatg gccgagcggg
     1921 cgaacgagta ggcgccgccg gtgtgcggct gggccgccgc catctcgccg atcgagaaga
     1981 tcattccgta gtacatgacc acgaggatga cgaacgcgat cagcattccg ccgaagccgg
     2041 cgaagtcgat gccgaagttc cagcccgaga agtcgcccga gatgaccgcg gcgacagcaa
     2101 ggccccagag gccccagact cccgcgcttc tcttgagact gcgcttctcg aagtagccgt
     2161 ccccggcttt cgtatacgtg acgccggaca ccttggtgcc gtctttctcc gccattcttc
     2221 ctccgtttgg gactgtcggg cgcaggcccc atcgcctgcg gaattcccat gagtttgtcg
     2281 gctattggta cctcgtgtct acctttggat gtaaattgcg cgttacggta gcccagtgat
     2341 cgaggcatcc ctcgtgatgt aatggtcgac acatactcga aggggagctt ctccaatggc
     2401 acacgcgagc acgcaaacga agacgggcaa cctgactgtt caggagctga ccgtgatggt
     2461 cgcggccaag gagatcgaca ccgtcatcat cgccttcacc gacatgcagg ggcgcctcgt
     2521 cggcaagcgc gcatccgctc gtctcttcct ggaggagctc gccgagcacg gcgccgagtg
     2581 ctgcaactac ctgctcgccg tcgatgtcga gatgaacacg gtgagcggat acgcgatctc
     2641 ctcgtgggag cggggctacg gcgacatggc gatgatgcct gacatcgaca ccctgcgtct
     2701 ggtgccgtgg ctgccgggca cggcgctcgt cacggccgac ctgacctggc tcgacggcac
     2761 acccgtcgcg ccggaccccc ggcaggtcct caagcgccag atcgaccgcc tggccaagaa
     2821 gggcctcagc tcgttcgtgg gcacggagct cgagttcatc gtcttcgagg acagctaccg
     2881 ggatgcctgg aagaagaagt acgtcgacct ctacccggcc agcgactaca acatcgacta
     2941 cgcgctgctc gcgtccagcc gcatggagcc gctgctgcgc gacatccgca acggcatgga
     3001 tggcgccggc atgtactgcg agggcgtcaa gggcgagtgc aatctcggcc agcaggagat
     3061 cgccttccgc tacacggacg cgctcggcac ctgcgacaac cactccgtct acaagaacgg
     3121 ggcgaaggag atcgccgacc agcacggcaa gtcgttgacc ttcatggcca agtacaacga
     3181 gcgcgagggc aactcgtgcc acatccacct ctcggtcgtg ggtgagaacg gcgagcccgt
     3241 catggccgac aaggatcggc cctacggatt ctcgaagctc atggagcact ggatcgccgg
     3301 ccagctggcg acactgcgtg agctcagcct gttcttcgcc cccaacatca actcctacaa
     3361 gcgcttcgtc gagggcagct tcgcaccgac cgccgtggcg tggggactcg acaaccgcac
     3421 ctgcgctctg cgcgtcgtcg gccacggacg cagcctgcgc gtggagaacc gcgtgcccgg
     3481 tggcgacgtc aaccagtacc tcgcggtcgc ggcgctcatc gcgggcgggc tctacggcat
     3541 cgagcatgag ctggagctgg agccgatctt cgagggcaac gcctacgtgt cggatgcccc
     3601 gcgcgtgccg tcatcgttgc gcgaggccgc ggcactcttc caggcgtccg aggtggccac
     3661 ggaggcgttc ggcgaggacg tcgtcgcgca ctacctcaac aacgcgcgca tcgagcaggc
     3721 ggcctacgac gccgcgatca cggattggga gcgggttcgt ggtttcgagc ggttctgagc
     3781 actccgccgg tcgagtagcc gccgacggcg gcgtatcgag acctggtttc gatacggctt
     3841 cgcctactca accggcccga cccgtcatcg ggctgacgac gtacctcgag caggcacaga
     3901 ccggggtgtg ggacgtgccc gcgagcttcc tgccgaaggt gtacttcgag gcggtcaccc
     3961 gtgccggggg catcgcggtg ctgctgccgc cgcagccggt cgacgctgag atcgccgacg
     4021 ccgtgctcga cggcctcgac gggctcatca tcacgggcgg caaggatgtc gaacccgccc
     4081 gctacggcca ggagccgcac cccaccaccg acgagcctcg ccgggatcgt gacgcctggg
     4141 aggatgccct gctgacggca gcgatcgacc gcggcctgcc gttcctcggc atctgccgcg
     4201 gggctcaggt gctcaacgtg gcactcggcg gcaccctgca ccagcacctg cccgacgtga
     4261 tcggcaccac gcgctacaac ctcggccagg gtcgcttcgc ggtcaacgag gtcggggtcg
     4321 aggcatccac gaagctcggg cagctgctgc agggtgacga gaccgtggac gtcaagagct
     4381 accaccatca agcgatcgac gaggtcgctc cgggactcgt cgtgtcggct cgcagtgacg
     4441 acggcacgat tcaggcggtg gagctggccg accatccctt cggggtggcc gtgcagtggc
     4501 atcccgagga ggacgccgcc gaagacgcgc gactcttcgc gggactcgtg gatgcagcga
     4561 aggagtaccg ctcggtggtc gagcgcgtcg aggccgaagg ggcacaccat ggctgagatc
     4621 tcgacaagct cgatcaccgg aacggcaagc tcgatcaccg gatcgacgac cctcatcgac
     4681 ccctcgacgg gtcaggcctt cgataccgtg catcagacct cacgcgagca ggtggatgac
     4741 gcgatcgcga gcgccgtcgt cgctcagcgc gcg
//
LOCUS       DAOSOU010000169         4750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_17991_length_4750_cov_7.639404, whole genome shotgun sequence.
ACCESSION   DAOSOU010000169 DAOSOU010000000
VERSION     DAOSOU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17991_length_4750_cov_7.639404"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLW15_09040"
     CDS             <1..570
                     /locus_tag="PLW15_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT34575.1"
                     /translation="TWADGTEYDLGGQWDRISMYESLNAASGLSVTPQTPVEELLAHA
                     ATLDIEVALPTHGKLVEELWEHYVKPGLTRPTFVMDFPVDTSPLVRDHRSIPGVVEKW
                     DLYVRGFELATGYSELVDPVIQRERFVAQALLASKGDPEAMRLDEEFLRALEHGMPPT
                     GGMGMGIDRLLMTVTGLGIRETILFPLVK"
     gene            594..740
                     /locus_tag="PLW15_09045"
     CDS             594..740
                     /locus_tag="PLW15_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34576.1"
                     /translation="MSDLKLPGEENKKKGEISFARIAIWVIVGGVGLYLVISGVVGII
                     VKGQ"
     gene            839..1021
                     /locus_tag="PLW15_09050"
     CDS             839..1021
                     /locus_tag="PLW15_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34577.1"
                     /translation="MPQEVWSNALFALAPTVLVGLVFWFIMAAIIRSDRSERSAKAKI
                     EAEERAKRGLPPKPAA"
     gene            complement(1057..>1820)
                     /locus_tag="PLW15_09055"
     CDS             complement(1057..>1820)
                     /locus_tag="PLW15_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT34578.1"
                     /translation="MDVPPHPHTGLQTVSWLFAGEIDHRDSVGTFAPIHPGQLNLMTA
                     GNGIAHSEVATDATDVLHGAQLWIALPEPDRHVAPFFESYVPVPVVVEGATLRVFLGS
                     LAGSTSSARVFTELLGAQLDVPAGTTVTLDVRPDFEHGVLLDSGEIALNGHPVPLSHL
                     AYVAPGSDTMTLTAGPTGATLLLIGGVPLGEQIVMWWNFIGRSHEEIVQFRTDWQSDV
                     IAGGNDNGRFGVVRGYDGRALPAPELPAVRLKPRG"
     assembly_gap    1821..1920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1921..2846)
                     /locus_tag="PLW15_09060"
     CDS             complement(<1921..2846)
                     /locus_tag="PLW15_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT34579.1"
                     /translation="MPGPSTYFDPSRIGELDPGERDLVARRARVLGSAYRLFYREPVQ
                     LVRGLGTRVWDAAGTEYLDAYNNVPSVGHSHPRVAEAIAKQAATLTTHTRYLSAGVVR
                     YSEQLLATLHPDIDNIIYTCTGSEANDLALRIAKLVTGGTGVIVTSNAYHGVTTEVAA
                     ISPSLGGVESIAGWVRLVDPADLGRQVPAALAEFSAAGIRPAALIVDTIFASDGVLPD
                     VADLGTAVAAVRAAGGLFIADEVQPGFGRLGQGMWGFDRYGIVPDMVTVGKPMGNGQP
                     IAAVMTRHELIDEFNRHSRYFNTFGGSSVSIAA"
     gene            complement(2846..3745)
                     /locus_tag="PLW15_09065"
     CDS             complement(2846..3745)
                     /locus_tag="PLW15_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT34580.1"
                     /translation="MDPVLEQLVEPFERMPPAEASRILTEHYGLPAAELESLPTERDD
                     SFLVSAGGQQFSLKVAHPSDDPAVIEMQLAAMTFAATADPGIPLQKIVLGLDGRPVQL
                     VKGRAARLLTWLPGELARGQEPTPSQLHASGVMLGRLNHALSTFEHPAAHRALAWDLR
                     HLGMLRELATDATAHVIDRFEVSVAPLLDGLPQQVIHNDFHPSNILVDERDPRYVVGV
                     LDFGDVVHAARVSDLGVALAYLAPQGDLLTGVQPFIDGFETVVPLLPEEKAILPDLVS
                     ARLVQRIVMSGLLAREQHTTGEN"
     gene            complement(3784..3942)
                     /locus_tag="PLW15_09070"
     CDS             complement(3784..3942)
                     /locus_tag="PLW15_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34581.1"
                     /translation="MSDDYDSRPVGFLGKLRNRKAITWVVIIALIVLTVGAGTIVAII
                     QFVLPGGN"
     gene            4083..>4750
                     /locus_tag="PLW15_09075"
     CDS             4083..>4750
                     /locus_tag="PLW15_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOT34582.1"
                     /translation="MFERFTDRARRVVVLAQEEAKMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLDAVRDQVQDIIGQGQQQPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNRVRQQVIQLLSGYQGKEAVAVGGNDATP
                     DKGSQILDQFGRNLTQAARDGKLDPVIGREKEMERVMQILSRRSKNNPVLIGEPGVGK
                     TAVV"
BASE COUNT          764 a         1547 c         1630 g          709 t
ORIGIN      
        1 acctgggccg acggcaccga gtacgacctc ggcgggcagt gggaccgcat ctcgatgtac
       61 gagtcgctca acgcggcatc cggtctcagc gtcacgccgc agactcccgt cgaggagctt
      121 ctcgcgcacg cggccacgct cgacatcgag gtggcgctgc ccacccacgg caagctcgtc
      181 gaggagctgt gggagcacta cgtcaagccg gggctcacgc gccccacgtt cgtcatggac
      241 ttccccgtcg acaccagccc gctcgtgcgc gaccaccgct cgatcccggg cgtcgtcgag
      301 aagtgggacc tctacgtgcg cggcttcgag ctggccaccg gctactccga gctcgtcgac
      361 ccggtcatcc agcgcgagcg cttcgtcgcc caggccctgc tcgcgagcaa gggcgacccg
      421 gaggcgatgc gcctcgacga ggagttcctg cgtgccctcg agcacgggat gccaccgacc
      481 ggcggcatgg gcatgggcat cgaccgcctg ctcatgacgg tcacggggct cggcatccgc
      541 gagacgatcc tgttcccgtt ggtgaagtga gcgccgtgaa gtgaggactg accatgagtg
      601 acctgaagct gcccggcgag gagaacaaga agaagggcga gatctcgttc gcccgcatcg
      661 ccatctgggt gatcgtcggc ggggtggggc tctacctggt gatctccggc gtcgtcggca
      721 tcatcgtcaa gggccagtag tggaccgtcg cacctcgcgg ctcgtgatgg gcatcgcgct
      781 cgggggcatc ctcatcgtga tcgccatcgc gttcctcatc accagctacg ctggttaggt
      841 gcctcaggaa gtctggtcga acgcgctctt cgcgctagcg ccgaccgtgc tggtcggtct
      901 ggtcttctgg ttcatcatgg cggcgatcat ccgttccgat cgctccgagc gctcggcgaa
      961 ggcgaagatc gaggccgagg agcgtgccaa acgcggtctc ccgccgaagc ctgccgcgta
     1021 ggccggtcga gtagggcgta gcccgtatcg aggcctctac cctctcggct tgagtcgcac
     1081 cgccggcaac tccggcgctg gcagcgcccg gccgtcgtag ccgcgcacga caccgaaccg
     1141 gccgttgtcg ttgccgccgg cgatcacgtc ggactgccag tcggtgcgga actgcacgat
     1201 ctcctcgtgg ctgcgcccga tgaagttcca ccacatcacg atctgctcgc cgaggggcac
     1261 gccgccgatc agcagcaggg tcgcgcccgt cggcccggcg gtgagggtca tcgtgtcgct
     1321 gcccggcgcg acataggcga gatgcgacag cggcaccgga tgcccgttca gagcgatctc
     1381 gcccgagtcg agcagcacgc cgtgctcgaa gtcggggcgc acgtcgagcg tgacggtggt
     1441 gccggccgga acgtcgagct gggcgccgag cagctcggtg aacacgcgcg ccgacgacgt
     1501 ggagccggcc agcgagccga ggaacacccg cagcgtcgcg ccctccacga cgaccggcac
     1561 gggcacgtag ctctcgaaga acggggcgac gtgccggtcg ggttcgggga gggcgatcca
     1621 gagctgggcg ccgtggagaa cgtcggtcgc atccgtcgcc acctcggagt gggcgattcc
     1681 gttgcctgcg gtcatcaggt tgagctggcc gggatggatg ggcgcgaagg ttcccacgct
     1741 gtcgcggtgg tcgatctcgc cggcgaacag ccagctcacc gtctgcagtc cggtgtgcgg
     1801 atgcggcggc acatccatgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 gcggcgatcg acaccgaact gccgccgaac gtgttgaagt agcgcgagtg ccggttgaac
     1981 tcgtcgatca gttcgtggcg ggtcatcacg gcggcgatcg gctgcccgtt gcccatgggc
     2041 tttccgactg tcaccatgtc gggcacgatg ccgtagcggt cgaaacccca catgccctga
     2101 cccagtcggc cgaagcccgg ctgcacctcg tcggcgatga acagacctcc cgccgcgcga
     2161 acggcggcga cggcggtgcc gaggtcggcg acatccggga gcacaccgtc ggaggcgaag
     2221 atcgtgtcga cgatcagagc ggccggccgg atgcccgcag ccgagaactc cgcgagggcc
     2281 gcgggcacct ggcggccgag gtccgcaggg tcgaccagcc gcacccagcc ggcaatggac
     2341 tcgacgccgc cgagtgacgg cgagatggcg gccacctccg tggtgactcc gtggtacgcg
     2401 ttgctcgtga cgatgacgcc ggtgccgccg gtcacgagct tggcgatgcg cagcgcgaga
     2461 tcgttggcct cggagcccgt gcacgtgtat atgatgttgt cgatgtcggg atgaagcgtt
     2521 gccagcaact gctccgagta ccgcacgacc ccggccgaca ggtagcgggt gtgagtggtg
     2581 agggtggcgg cctgcttggc gatggcctcc gcgacccgcg ggtgggagtg gccgacggac
     2641 ggcacgttgt tgtaggcgtc gaggtactcg gtgcccgcgg catcccacac ccgggttccg
     2701 aggccacgca ccagttgcac cggctcacgg tagaagagtc ggtaggcgga gcccagcacc
     2761 cgagcacggc gggcgacgag gtcgcgctcg cccgggtcga gctcgccgat gcggctcgga
     2821 tcgaagtagg tcgatggtcc cggcatcagt tctcccccgt ggtgtgctgc tcccgtgcca
     2881 gcaatcctga catcacgatg cgctgcacca gccgcgccga cacgaggtcg ggaagaatcg
     2941 ccttctcctc cgggagcagg ggaacaaccg tctcgaatcc gtcgatgaac ggctgcacac
     3001 ccgtgagcag gtcaccctgc ggcgcgagat aggccagcgc cacccccaag tcgctcacgc
     3061 gggccgcgtg caccacatcg ccgaagtcga gcacgccgac gacgtaccgc gggtcgcgct
     3121 cgtccacgag gatgttcgag gggtggaagt cgttgtggat gacctgttgg ggcaggccgt
     3181 cgagcagggg cgccacgctg acctcgaacc ggtcgatcac gtgggcggtc gcgtcggtgg
     3241 cgagttcgcg cagcatcccg aggtgacgca agtcccaggc cagggcgcgg tgggcggccg
     3301 gatgctcgaa ggtgctgagc gcgtggttga ggcgacccag catcacgccc gacgcgtgca
     3361 gctgcgacgg cgtcggttcc tgtccgcgcg cgagctcgcc cggcagccag gtgagcaggc
     3421 gcgcggcgcg ccccttgacg agctgcaccg gtctgccgtc gagcccgagc acgatcttct
     3481 gcagcgggat gcccgggtcg gccgtcgccg cgaacgtcat cgccgcgagc tgcatctcga
     3541 tgaccgcagg gtcgtcggag gggtgggcga ccttgagcga gaactgctgt ccgccagccg
     3601 agaccaggaa gctgtcgtcg cgttcggtgg gcagcgactc gagctcggcg gcgggcagcc
     3661 cgtagtgctc ggtcagaatg cgggatgcct cggcgggcgg catccgctcg aagggttcga
     3721 ccagctgctc cagtaccgga tccatcaccc cagcttaggc agggggtcgc gcgcggtgga
     3781 gggtcagttg ccgccgggta gcacgaactg gatgatcgcg acgatcgttc cggcgccgac
     3841 ggtcagcacg atgagggcga tgatgaccac ccaggtgatg gccttgcggt tgcgcagctt
     3901 gcccaggaaa ccgaccgggc gggagtcgta atcgtcactc atgtcagcga gcctagggga
     3961 tggcgccgga gggcgcgatc gcctgtcacg cctacggcga acaccggact attcgcgcct
     4021 gcccgtggtt agtctcttta catcggtacc gaccacgaac ggtgccggcc aaggagtaga
     4081 agatgttcga gagattcacg gaccgcgccc gtcgcgttgt cgtcctggcc caggaagagg
     4141 ccaagatgct caaccacaac tacatcggta ccgagcacat cctgctcggg ctcatccacg
     4201 agggtgaggg cgttgccgcg aaggcactcg agtccctcgg catctctctg gatgccgtgc
     4261 gcgaccaggt gcaggacatc atcggccagg gccagcagca gccgaccggg cacatcccgt
     4321 tcacgccgcg cgccaagaag gtgcttgagc tgagcctgcg cgaggcgctg caactcggcc
     4381 acaactacat cggcaccgag cacatcctgc tcgggctcat ccgcgagggc gagggcgtcg
     4441 cagcccaggt gctcgtgaag ctgggcgccg acctcaaccg agtgcgccag caggtcatcc
     4501 agctgctctc cggctaccag ggcaaggagg cggtcgccgt cggcggcaac gacgccactc
     4561 ccgacaaggg cagccagatt ctcgaccagt tcggtcgcaa cctgacgcag gccgctcgtg
     4621 acggcaagct cgaccccgtg atcggccgcg agaaggagat ggagcgggtc atgcagatcc
     4681 tctcccgccg ctccaagaac aaccccgtgc tgatcggtga gcccggcgtc ggcaagaccg
     4741 ccgtcgtcga
//
LOCUS       DAOSOU010000170         4748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18007_length_4748_cov_5.916898, whole genome shotgun sequence.
ACCESSION   DAOSOU010000170 DAOSOU010000000
VERSION     DAOSOU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18007_length_4748_cov_5.916898"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PLW15_09080"
     CDS             <1..1487
                     /locus_tag="PLW15_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOT34583.1"
                     /translation="VAGRPKQYYSIYQKMIVRGREFDEIYDLVGIRVLVNSVRDCYAV
                     LGSIHARWNPIPGRFKDYIATPKFNLYQSLHTTVIGPEGRAVEIQIRTHEMHQRAEFG
                     VAAHWKYKARAKGEDAGGRHDDADMAWLAHITDWQSETEDPGEFLDSLRFEIGAKEVY
                     VFTPQGKVIGLPAGATPVDFAYAVHTEVGHRTMGAKVNGRLVPLESALNSGDSVEVFT
                     SKSPDAGPSQDWLNFVKSTRARNKIRAWFTKERREEAIEQGKDAIARAMRKQNLPLQK
                     LMSQDSFAEVAAQMRYDDVSALYAAVGEGHVSTQSVIEKVLASLHSEAETEDSDIEFA
                     PKGRSRALKNSDSGVLVRGAPDILVKLAKCCTPVPGDDIVGFVTRGAGVSVHRSDCNN
                     VASLLQEPERMIDVEWAPTSKTLFLVQIQVEALDRAGLLSDITRVLSENHVNILSATV
                     STSSDRLALSRYVFEMGDTTHLDRVLNAVRRIDAVYDVYRVNSG"
     gene            complement(1484..2032)
                     /locus_tag="PLW15_09085"
     CDS             complement(1484..2032)
                     /locus_tag="PLW15_09085"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOT34584.1"
                     /translation="MTPRSPLVLSADDLPLAELLAARLDGEVFGIGSGFASVAELEQP
                     QHRAASLGAANSRLIAEQRTAAWIWGVLTRAPERHEFCSTLTERVAHQSSRFITVREV
                     VLDPDDLITLAGRQVTTPLRTCLDLARFSAEFGRPERAIVRDLMSLARVGMLDFTRHL
                     NRRRNLPHKTVALARLTECVAD"
     gene            complement(2103..3332)
                     /locus_tag="PLW15_09090"
     CDS             complement(2103..3332)
                     /locus_tag="PLW15_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOT34585.1"
                     /translation="MTDETAPWGRVDETGTVYVREAAGERVVGQYPDGTPEEALAYFE
                     RKFTELAGQVTLLEQRVKRGAPAADVAKTVKTLGASIAGANAVGNLAALQARIEALGG
                     TVSELTEKQSEEAKALVAEALAEREALVVEAETLAAQDPAKAQWKQVTATLDDIFARW
                     QKHQSDGPRLPKTESNELWKRFRAARATIDGHRKAFFAELDSVHKDARATKQALVEKA
                     EALISKGADGIPAYRALLDDWKAAGRAGKKFDDALWAKFKDAGDALYSAKSEIDAKDS
                     VEFEANLALKLELLAEAEPLVTETDRVKAKEALLSIQKRWDKIGKVPREQVKVVEDRL
                     RKVEAAVRKLDEEHWQRNNPERQQREEGFLGQLTEAIAKLERELEAAKAGGDKKKIAE
                     AQEALDARRAWLGALKN"
     gene            complement(3421..4212)
                     /locus_tag="PLW15_09095"
     CDS             complement(3421..4212)
                     /locus_tag="PLW15_09095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT34586.1"
                     /translation="MAASKNTEREAREARERLRRYNARQAVHENQLKRRKRDNWYAVG
                     VLVIVASAAAVLQIFYFNGGPGTPTPVPSASETAPPVTGQNVGDVPDPSFAQGKTYTG
                     TLGLNDIELGITLDGALAPQAVSSILADSLTGYYVGKTCHRLVVSTGAGLLQCGSLDG
                     TGATDPSYSFGPIENAPADGIYPAGTIAMARASGDAYSNGRQFFIVFQDSTLPNDAAG
                     GYTVVGSVTSGLDSLMAQIANAGTADGSTDGAPLVATTITSLTIQ"
     gene            4307..>4748
                     /locus_tag="PLW15_09100"
     CDS             4307..>4748
                     /locus_tag="PLW15_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34587.1"
                     /translation="MNAQTGLRGGSTPLAVRMRPTRLDEIAGQRHLLTPGSPLVSLAG
                     EGSGEQGSVSIILWGPPGTGKTTLAKAIAHSSGRKFVELSAVTAGVRDVRQVMEEALS
                     SRDLYGLTTVLFLDEIHRFTKAQQDALLPGVENGWVILIAATTEN"
BASE COUNT          767 a         1567 c         1563 g          851 t
ORIGIN      
        1 gcgtcgccgg ccggccgaag cagtactact cgatctacca gaagatgatc gtgcgaggcc
       61 gtgagttcga cgagatctat gacctcgtcg gcattcgggt tctggtgaac tcggtgcgcg
      121 actgctacgc ggtactgggg tcgattcacg cccgttggaa cccgattccg ggtcgcttca
      181 aggactacat cgccaccccg aagttcaacc tctaccagtc gctgcacacg acggtgatcg
      241 gcccggaggg ccgcgctgtc gagatccaga tccgtaccca cgagatgcac cagcgcgcgg
      301 agttcggtgt cgcagcccac tggaagtaca aggctcgcgc gaagggcgag gatgccggcg
      361 gtcgccacga cgacgccgac atggcctggc tcgcgcacat caccgactgg caatccgaaa
      421 ccgaggaccc gggggagttc ctcgatagcc tccgcttcga gatcggcgcc aaagaggttt
      481 acgtcttcac cccgcagggc aaggtcatcg gccttcccgc cggggcaacg cccgtcgact
      541 tcgcctatgc cgtgcacacc gaggtcggtc accgcaccat gggtgccaag gtcaacggtc
      601 gtctggttcc gctcgagagc gcgctcaaca gtggcgactc ggtcgaggtc ttcacctcga
      661 agagcccgga cgccgggccc agccaggact ggctgaactt cgtcaagagc acgcgcgccc
      721 gcaacaagat cagggcctgg ttcacgaagg agcgccgcga ggaggcgatc gagcagggca
      781 aggatgcgat cgcccgtgcc atgcgcaagc agaatctccc gctgcagaag ctcatgagcc
      841 aggactcgtt cgccgaggtg gccgcacaga tgcgctacga cgatgtttcg gcgctctacg
      901 ccgccgtcgg cgagggacac gtgtcgacgc agtccgtgat cgagaaggtt ctcgcgtcgc
      961 tgcacagcga ggccgagacc gaggattcgg acatcgagtt cgcgcccaag ggcaggagtc
     1021 gcgcgctcaa gaacagcgac tccggagtgc tggtgcgcgg ggcgcccgac atcttggtca
     1081 agctggccaa gtgctgcacc cccgttccgg gcgacgacat cgtcgggttc gtcacgcgag
     1141 gggccggggt gtcggtgcat cgaagtgact gcaacaacgt ggcatccctg cttcaggaac
     1201 ccgagcgcat gatcgacgtc gagtgggcac ccacgtcgaa gacgctgttc ctggtgcaga
     1261 tccaggtcga agccctcgac cgggcgggcc tgctgtctga catcacgcgc gtgctcagcg
     1321 agaaccacgt caacatcctg tcggcgactg tctccacgtc gagcgacaga ctggccctca
     1381 gccgctacgt cttcgagatg ggtgacacga cccacctcga ccgggttctc aatgcggtgc
     1441 gtcgcatcga cgccgtgtac gacgtgtatc gggtgaactc gggctagtcg gctacgcact
     1501 cggtgagcct cgcgagcgcc acggtcttgt gcgggaggtt gcgtcgccgg ttgaggtgac
     1561 gggtgaagtc cagcatgccc accctggcga ggctcatgag gtctcggact atcgcgcgct
     1621 ccggccgacc gaactccgca ctgaagcgcg cgagatccaa gcaggtgcgc aagggcgtgg
     1681 tcacctgtct gcccgccagg gtgatgaggt cgtcggggtc gagcacaacc tcacgcacgg
     1741 tgatgaaacg tgacgactgg tgggcgaccc gctctgtgag ggtcgaacag aactcgtggc
     1801 gttccggtgc ccgcgtcaga actccccaga tccacgccgc ggtgcgctgc tccgcgatca
     1861 agcgggagtt ggcggcccca agtgaggccg ctcggtgctg aggctgttcc agctcagcaa
     1921 ccgatgcgaa accggatccg atgccgaaga cctcgccgtc tagccgggcc gcgagcagct
     1981 cagcgagcgg aaggtcgtca gcggacaaga cgaggggcga gcgcggagtc atccctcgag
     2041 catgacgagc ccttatcgtc gcgactactc acggccgcca atccgtgcac gaatgccggt
     2101 tgttagttct tgagcgcacc gagccacgcg cggcgggcgt cgagcgcttc ctgggcctcg
     2161 gcgatcttct tcttgtcgcc accggccttc gctgcctcga gctcgcgctc gagcttggcg
     2221 atcgcctcgg tgagctgtcc gaggaagccc tcttcgcgct gctgccgttc gggattattg
     2281 cgctgccagt gttcctcgtc gagcttgcga accgcggcct cgaccttgcg cagccgatcc
     2341 tcgacgacct tgacctgctc gcgcggaacc ttgccgatct tgtcccagcg cttctgaatt
     2401 gacagcagcg cctccttggc cttgacgcgg tcggtctcgg tcacgagcgg ctcggcttcg
     2461 gcgagcaact cgagcttgag cgcgaggttc gcctcgaact cgacactgtc cttcgcgtcg
     2521 atctccgact tcgcggagta gagggcgtca cccgcgtcct tgaacttcgc ccacagggcg
     2581 tcgtcgaact tcttgccagc gcgtccggcg gccttccagt cgtcgagaag cgcgcgatag
     2641 gccgggatgc cgtcggcacc cttcgagatg agggcttctg ccttctccac gagtgcctgc
     2701 ttcgttgccc gcgcgtcctt gtgcacgctg tcgagctcgg cgaagaacgc cttgcggtgt
     2761 ccgtcgatgg tggctcgggc cgccctgaat cgcttccaca gttcgttgga ctcggtcttc
     2821 ggcagacggg gaccgtcgga ctgatgcttc tgccagcgcg cgaagatgtc gtcgagggtg
     2881 gctgtgacct gcttccactg cgccttcgct ggatcctggg cggcgagggt ctccgcttcg
     2941 acgaccagcg cctcgcgttc ggcgagtgcc tctgcgacca acgccttggc ctcttcgctc
     3001 tgcttctccg tgagctcgct cacggtgccg ccgagtgcct cgatgcgtgc ctgcagcgcg
     3061 gcgagattgc ccacggcgtt cgcgccggca atgctcgcac ccagcgtctt cacggtcttg
     3121 gccacgtcgg ccgccggtgc gccgcgcttg acgcgctgct caagcagggt tacctggccg
     3181 gcgagttcgg tgaacttgcg ctcgaagtag gcgagggctt cctcgggagt gccgtcggga
     3241 tactgcccca cgacacgctc gcccgcggcc tcgcgcacat agacggtgcc cgtctcatca
     3301 acgcggcccc acggagccgt ctcgtcagtc acgtgattca ccttgctcga aaggatgtct
     3361 gctacctagc ccagcgggcg ttcaccagct tatggcaagc ggcccgggcc tccgactgcg
     3421 ttactggatc gtgaggctcg tgatcgtggt cgcaaccaga ggcgcgccat cggtcgaacc
     3481 gtcggctgtt cccgcgttgg cgatctgggc catcaggctg tcgaggccgc tcgtgacact
     3541 tccgacgacg gtgtagccgc ccgcagcgtc attgggcagc gtcgagtcct ggaacacgat
     3601 gaagaactgc cgaccattgc tgtaggcgtc tcccgacgcg cgcgccatcg cgatcgtgcc
     3661 ggccgggtag atgccgtctg ccggggcgtt ctcgatcggg ccgaagctgt acgaggggtc
     3721 agtggcccct gtgccgtcta gagagccgca ctgaaggagc ccggcgccgg tgcttacaac
     3781 cagtcggtga caggtcttgc cgacgtagta gcccgtcagg gagtcagcga ggatgctcga
     3841 gacggcctgg ggtgccaggg caccgtcgag ggtgatcccg agctcgatgt cgttgagtcc
     3901 cagggtgccg gtgtaggtct tgccctgcgc gaagctcgga tcgggcacgt caccgacgtt
     3961 ctgacccgtc accggcggcg ccgtctcgct cgccgaggga accggcgtcg gagttcccgg
     4021 accgccattg aagtagaaga tctggagcac ggcggccgcg gatgcaacga tcacgagcac
     4081 tcccaccgcg taccagttgt cgcgcttacg acgcttgagc tggttctcgt gcacggcctg
     4141 ccgggcgttg tagcggcgca ggcgctcgcg tgcctcgcgt gcctcacgct cggtgttctt
     4201 gctcgcagcc acggctgatc tccttcggga tgatgcttgg ggtcgagtaa ctttacgaca
     4261 gtcaggccga atggtgccgc cggtggccgc ggctagcatt gccgctatga acgcacagac
     4321 cggactgcgc ggtgggtcaa ccccgctcgc ggtacggatg cgccccactc gactcgacga
     4381 gattgccggt caacggcacc tgctgacgcc gggctcgccc ctcgtcagcc tcgcggggga
     4441 gggcagcggc gagcagggat cggtgtcgat catcctctgg gggcctcccg gcacaggcaa
     4501 gaccacactg gccaaggcga tcgctcacag ttccggccgt aagttcgtcg agctctccgc
     4561 cgtcaccgcc ggcgttcggg acgtccgtca ggtgatggag gaggccctgt cgagccgtga
     4621 cctctacggg ttgaccacag tcctcttcct cgacgagatc catcggttca ccaaggcaca
     4681 gcaggacgcg ctgctgcccg gcgttgagaa cggctgggtc atcctgatcg cagccaccac
     4741 cgagaacc
//
LOCUS       DAOSOU010000171         4685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18323_length_4685_cov_5.269762, whole genome shotgun sequence.
ACCESSION   DAOSOU010000171 DAOSOU010000000
VERSION     DAOSOU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18323_length_4685_cov_5.269762"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLW15_09105"
     CDS             <1..766
                     /locus_tag="PLW15_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT34588.1"
                     /translation="AITSGKGGVGKSTLTANLGVALAARGLSVGIVDADVFGFSIPGL
                     LGVAGVKPTKVGEMILPPVAHDVKVISIGMFVDGSQAVSWRGPMLHRTIQQFLTDVFF
                     GDLDVLLIDLPPGTGDVAISLGQLLPQAEVIVVTTPQAAAAEVAERSALVARQTGQSV
                     IGVVENMAGLVQPDGSVLELFGSGGGADAAARLGVPVLASVPLSVALREGGDAGQPVV
                     LSDPEDAAARVILSLADQLATRERGLAGRKLGLSPR"
     gene            1061..1975
                     /locus_tag="PLW15_09110"
     CDS             1061..1975
                     /locus_tag="PLW15_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT34589.1"
                     /translation="MDIRRLELLRELADRGSVTAVAAATHRTASAVSQQLKILEREAG
                     VPLTERAGRGIALTSAGRALARTATEVATAIERAEALWEEFRAQPRGEVSLVIFPTGG
                     QMLLPGLLTTVDGMPGLTLHAGDLDSTVGDIADLTADYDVVVADSPGVLPGWSERGLA
                     VVQLMREPLDIALPEGHPLGARSSLSPADLADQTWIGTPEGLPFDRILRRIEGANGTP
                     ARIAQRFADNGIVESLVAAGHGIAILPRYTTRDRENGLITRPLTGVRASRLISVLMRP
                     DRAERPSVRAVVTALRDEAARFEAQHTG"
     gene            complement(1994..3478)
                     /locus_tag="PLW15_09115"
     CDS             complement(1994..3478)
                     /locus_tag="PLW15_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT34590.1"
                     /translation="MRFAEDPFAILARLDELRAADAPTHGGHVLSYVYDSGLAELDEL
                     AASAIRAVQPVNGLDPTTFTSVAVMEREVVAFARDLLNGDDDVVGSVTTGGTESCLLA
                     VKTARDVWAAGAGSPVSTGSTTRPRLLAPVTVHAAFQKAAHYFGLELDLVPVLADGRV
                     DADVLIERMGADVALVVVSAPSYPHAALDPIAQVAAAAAERGIACHVDACIGGLVLPF
                     WPDLPEWDFRVPGVTSISADFHKFGYAPKGVSVLLTRGRDRQRAQYFATTRWPGYPVV
                     NPTILGSKSAGALAAAWAITRALGREGFAELAASCHRSTTALVELVETIPGLRVVGSP
                     VGPLLAVATDATAPADQQVDPHHWADQARAHGWHLQLQPGLTQPDGTTLPRTTHLTIT
                     PVTESVLPELSAALVTAADEVRGVPGVSSEQVLASLPPGLAAAPLDAPLDAATAFGIL
                     QSIGLVSAGGGLPDRMAPFIALVEALPAPLTQRLLTELIARTFE"
     gene            complement(3475..>4685)
                     /locus_tag="PLW15_09120"
     CDS             complement(3475..>4685)
                     /locus_tag="PLW15_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34591.1"
                     /translation="IAALAAGVLVTIAKVWDVIIDPVIGARSDRSLARHGSRRPYMLA
                     GAIALPVFFVLTFAVPAAVGPVIGGIWVLLAFMITATAFSLFQVPYIALPAELASGYD
                     ERTRLISARVVVLTIAILLFGAGAPEIRDSFDDEHLGYLVMAIVAGLVIGLGMFISSF
                     VARKGAPTEVPQFSVADNYRKGLHALRRSQPFRALLLTFLLQGLATGLMLAGAQYVAR
                     WVLDTGVTILFLSLIAPAILFAPLWAIVSRRIGKERTFFVASALFGIAALSMTLLIWF
                     PGAWLYAPVALAGAGYAGMQSLPMSMLPDVISHDAGTHGQGSAGTFGGVWTAGETTGM
                     ALGATVLTIVLAASGYLESVGNQVVVQPGSAIVGIVLSFSVVPAAIIAASLISFARYP
                     LRKADIDVAA"
BASE COUNT          759 a         1621 c         1667 g          638 t
ORIGIN      
        1 cgccatcacg agcggcaagg gcggcgtcgg caagtcgaca ctcaccgcga acctcggggt
       61 cgccctggcc gcgcgggggc tgtcggtggg catcgtggat gccgacgtgt tcggtttctc
      121 gatccctggg ctgctcgggg tggccggggt gaagcccacc aaggtgggcg agatgatcct
      181 cccgccggtc gcgcacgacg tgaaggtaat ctcgatcggc atgttcgtcg acggatcgca
      241 ggccgtctcc tggcgcggac caatgctgca tcgcacgatc cagcagttcc tcaccgatgt
      301 gttcttcggc gacctcgacg tgctgctcat cgacctgccg cccggaacgg gcgacgtcgc
      361 gatcagcctg gggcagctgc taccgcaggc cgaggtgatc gtcgttacga ccccgcaggc
      421 cgcggcggcc gaggtggccg agcgcagcgc gctcgtggcc cgtcagaccg ggcagagcgt
      481 gatcggggtg gtcgagaaca tggcggggct ggtgcagccc gacggcagcg ttctcgagct
      541 gttcggctcg ggcggtgggg cggatgccgc ggcgcggctg ggagttccgg tgctggcctc
      601 ggttcccctg agcgtcgccc tccgcgaggg cggggacgcg gggcagcccg tggtgctgag
      661 cgaccccgag gatgctgcgg cgcgcgtcat cctgtcgctg gcggatcagc tggccacgcg
      721 tgaacgcggg ctggcggggc gcaagctggg gctcagcccg cgctgacgcc ccggtgatcg
      781 agcgaagtcg agatcagccc gaccggtgat cgagcaaagt cgagatcagc ccgaccggtg
      841 atcgagcgaa gtcgagatct cgacaggctc gatcagcgat gagttcagcg ggtcggcacg
      901 cggcggtaac cgtcgcggat gacgagaacg atggagccgg cgatgccggc gagggagagg
      961 gctgcgagaa cgatgagatc gggagacatg tgtcaattca agccgtgaca accatgtaga
     1021 acaagctcac ctttctaaag taactatgta ggctatcttc atggatatcc gccgcctcga
     1081 gctgctcaga gagctcgccg accgcggcag tgtcacggcg gtcgcggcgg cgacgcaccg
     1141 aactgcatcc gctgtttcgc agcagttgaa gatcctcgag cgcgaggccg gcgtgccgct
     1201 gaccgagcgt gcaggccgcg gcatcgctct cacctcagcg ggccgcgcgc tggcccgcac
     1261 cgcgacggag gttgccactg cgatcgagcg cgccgaggcg ctgtgggagg agttccgcgc
     1321 ccagccgcgc ggtgaggtgt cgctggtgat cttcccgacc ggcgggcaga tgctgctgcc
     1381 ggggttgctg accacggtcg acgggatgcc cggactcacc ctccacgccg gcgacctcga
     1441 ctcgaccgtc ggcgacatcg ccgacctcac ggccgactac gacgtcgtcg tcgccgactc
     1501 ccccggcgtg ctccccggct ggtcggagcg cggcctcgct gtcgtgcagc tcatgcgcga
     1561 gccgctcgac atcgccctgc ccgagggcca tcccctcggg gcccgttcct cactctcccc
     1621 tgccgatctc gccgaccaga cctggatcgg cacgcccgag ggcctcccct tcgaccgcat
     1681 ccttcgacgc atcgaggggg cgaacggcac ccccgcgcgc atcgcacagc ggtttgccga
     1741 caacggcatc gtcgagtcgc tcgtcgccgc cggccacggc atcgcgatcc tgccgcgcta
     1801 cacgacccga gatcgggaga acgggctcat cacacgcccg ctcaccggag tgcgggcatc
     1861 ccggctcatc tccgtgctca tgcgtcccga ccgcgccgag cggccctcgg tgcgcgccgt
     1921 cgtcaccgcg ctgcgcgacg aggcagcccg cttcgaggcg cagcacaccg gctgacggat
     1981 gccgcggcgc gacctactcg aacgtgcggg cgatgagttc cgtgagcagc cgctgggtga
     2041 ggggggcggg cagggcctcc acgagtgcga tgaatggcgc catgcgatcg ggcagcccgc
     2101 cgcctgccga cacgagcccg atcgactgaa ggatgccgaa ggcggtcgct gcgtcgaggg
     2161 gcgcatcaag cggggcggcc gcgagccccg gaggcagtga cgcgagcacc tgctccgagg
     2221 agacgccggg aacgccccgt acctcgtcgg ccgccgtgac gagcgccgcc gacagctcgg
     2281 gcagcaccga ctcggtgacc ggcgtgatcg tcaggtgcgt cgtacgcggc agcgtggttc
     2341 cgtcgggttg ggtgagtccg ggctggagct gcaggtgcca cccgtgcgca cgagcctggt
     2401 cggcccagtg gtgggggtcc acctgctggt cggcgggagc cgtggcatcc gtggccaccg
     2461 ccaggagcgg gcccacgggc gaaccgacga ctcgcagtcc agggatggtc tcgacgagct
     2521 cgacgagcgc cgtggtcgac cggtggcagg atgccgcgag ctcggcgaac ccctcgcgcc
     2581 cgagcgcgcg cgtgatcgcc cacgccgccg cgagcgcccc cgccgacttg ctgccgagga
     2641 tcgtcgggtt gaccaccgga taccccggcc agcgggtcgt ggcaaagtac tgcgcacgct
     2701 ggcggtcacg cccgcgggta agcagcaccg acacgccctt gggcgcgtag ccgaacttgt
     2761 ggaagtccgc gctgatgctg gtgacgcccg gcacgcggaa gtcccactca ggcaggtcgg
     2821 gccagaacgg cagcacgagt ccgccgatgc acgcgtcgac gtggcaggcg atgccacgct
     2881 cggcggcggc ggccgccacc tgcgcgatgg ggtcgagagc cgcgtgcggg tagctcggcg
     2941 cagagacgac gacgagcgcg acatccgcgc ccatgcgctc gatgagcacg tcggcgtcga
     3001 ctcggccgtc ggccagcacg ggaacgagat cgagctcgag cccgaagtag tgcgcggcct
     3061 tctggaacgc cgcgtgcacg gtgaccggcg ccagcagccg cggtctggtg gtcgagcctg
     3121 tcgagaccgg cgagcccgca cccgccgccc acacatcccg cgccgtcttg accgcgagca
     3181 ggcaactctc cgtgccaccc gtggtgacgc tgcccaccac gtcgtcatca ccgttgagca
     3241 ggtcgcgggc gaacgccacc acctcgcgct ccatgaccgc gaccgacgtg aacgtcgtcg
     3301 ggtcgagccc gttgacgggc tgcaccgcgc ggatcgcgga tgccgcgagt tcgtcgagct
     3361 cggcgagacc cgagtcgtag acgtagctga gcacgtgacc gccgtgggtc ggcgcatccg
     3421 cggcccgcag ctcgtcgagg cgggccagga tcgcgaacgg gtcctcggcg aacctcatgc
     3481 tgccacgtcg atgtctgcct tgcgcagcgg atagcgcgcg aacgagatga gcgaagccgc
     3541 gatgatcgcc gctggcacga cgctgaagct cagcacgatt cccacgatgg ccgaacccgg
     3601 ctgcacgacc acctggttgc ccaccgattc gaggtaaccg gatgccgcga gcacgatggt
     3661 cagcaccgtc gcccccaacg ccatcccggt cgtctccccc gccgtccaca cgccgccgaa
     3721 ggtgccggcg ctgccctggc cgtgggtgcc tgcgtcgtgc gagatcacat cgggcagcat
     3781 ggacatcggc agcgactgca tgcccgcgta tcccgctccg gcgagcgcga ccggcgcgta
     3841 gagccaggcg cccgggaacc agatcagcag cgtcatcgag agggccgcga tgccgaacag
     3901 agccgaagcc acgaagaagg tgcgctcctt gccgattcgc ctcgagacga tcgcccacag
     3961 cggtgcgaac aggatcgccg gcgcgatgag cgacaggaac aggatcgtca caccggtgtc
     4021 gagcacccag cgggcgacat actgtgcgcc ggcgagcatg agtcccgttg cgagcccctg
     4081 cagcaggaag gtcagcagca gggcacggaa cggctggctg cggcgcagcg cgtgcaggcc
     4141 cttgcgatag ttgtcggcga ccgagaattg cggaacctcg gtcggcgcgc ccttgcgagc
     4201 gacgaacgag ctgatgaaca tgccgaggcc gatcaccagg cccgcgacga tcgccatcac
     4261 gagatagccg aggtgctcgt cgtcgaacga gtcgcggatc tccggcgccc cggcaccgaa
     4321 cagcaggata gcgatcgtga gcacgaccac ccgcgccgag atcaggcgag tgcgctcgtc
     4381 gtagccgctg gcgagttcgg cgggcagcgc gatgtacggc acctggaaca gactgaaggc
     4441 ggtcgccgtg atcatgaacg cgagcagcac ccagatgccc ccgatgaccg ggcccaccgc
     4501 cgcgggtacg gcaaaggtca gcacgaagaa gaccggcagc gcgattgctc ccgccagcat
     4561 gtacggtcgg cgcgaaccgt ggcgggccag gctccgatcg ctgcgcgcgc cgatcacggg
     4621 atcgatgatc acatcccaca ccttggcgat cgtcacgagc acgccggcgg cgagcgcggc
     4681 gatgc
//
LOCUS       DAOSOU010000172         4666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18422_length_4666_cov_6.958794, whole genome shotgun sequence.
ACCESSION   DAOSOU010000172 DAOSOU010000000
VERSION     DAOSOU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18422_length_4666_cov_6.958794"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLW15_09125"
     CDS             complement(<1..830)
                     /locus_tag="PLW15_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT34592.1"
                     /translation="MSAAASSGAGATASELIRLSAADLASKLASGEVSSVEATQAHLD
                     RIAAVDGDVHAFLHISADALATAERIDQQRAAGDTLGPLAGVPVAIKDVLCTLDMPST
                     AGSKILEGWIPPYDATTVARLRAAGLVPLGKTNMDEFAMGSSTEHSAYGPTKNPWDLT
                     RIPGGSGGGSAAAVAAFEAPLALGSDTGGSIRQPAAVTGSVGVKPTYGGVSRYGAIAL
                     ASSLDQVGPVTRTVLDAGLLHDVIGGHDPRDSTSLKDVWPSFAEAARAGARAESLKGL
                     R"
     gene            complement(827..1126)
                     /gene="gatC"
                     /locus_tag="PLW15_09130"
     CDS             complement(827..1126)
                     /gene="gatC"
                     /locus_tag="PLW15_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOT34593.1"
                     /translation="MSEITPDVVTHLASLARIALTDEEIQTLTGELGAIVDSVAKVSE
                     VATPDVPATSHPIPLVNVYRDDVPGVTLTTEQALAGAPEHDGSRFQVSAILGEEQ"
     gene            complement(1204..1641)
                     /locus_tag="PLW15_09135"
     CDS             complement(1204..1641)
                     /locus_tag="PLW15_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34594.1"
                     /translation="MVVALACAAASLTGCAVAAATADEVHRDFARVLVTTQELVGGDW
                     DVRDDPDSRACANSHGVAGLQVPALRVAAGTSPTGPADAARAVAEQWSALGYEVTQTP
                     TLDGPDVVEVQARGDDGEYLIFRASDQAMTIQGESACVASAEG"
     gene            complement(1801..3237)
                     /locus_tag="PLW15_09140"
     CDS             complement(1801..3237)
                     /locus_tag="PLW15_09140"
                     /inference="COORDINATES: protein motif:HMM:NF018018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34595.1"
                     /translation="MLFDVTAVLTTSAALGSVAEAFGVHEIARYATSISTQENVPAPL
                     SLTTITLAAPDGAGSDLVGSELPGLPPQPFIGPVVPESGGATTLGTAPTGRDGAIGSP
                     AEVVKGTQLLGQIALLPVGQIDQFVRDNPEAIASLIGNPPRSQDVALWWGSMGLDARN
                     ALRVATPQLVGNLDGIPYQVRDLANRSVLTSTMRGLDYTIRTELGRTAVEKAKLQLSM
                     LKSISNALGDASGNTNRTLMSLDVTGQGRAAIIVGDLATADYITYMVPGMFFTIEGQM
                     VYWTDAAARLRDEQQSWLAHYGDTTSTVATVAWIGYHTPNLTNVGSLENAEEGRDSLA
                     RSIEGLQTLRSASEPYVTIVGHSYGSTAALMALTEYDFEVDALAVVGSPGSAAQSVRQ
                     LHVKNGNVFVGKAQWDPVPNSAFFGSEPGSASYGATTFGTDGGTDPLTGQTMLRSFFH
                     IEYFSPATESLRNLALIGIGHGDDVTPQ"
     gene            complement(3614..>4666)
                     /locus_tag="PLW15_09145"
     CDS             complement(3614..>4666)
                     /locus_tag="PLW15_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT34596.1"
                     /translation="CPECGTALAPAKEGDIDLRCPNARSCPAQVRGRVEHVGSRGALD
                     IEGLGEVSAAALTQPLVPATPPLVTEAGLFSLTLEQLFPITVVVRDNETGLPRQVGDD
                     EPGTQITTGDGVTLRVKTETPFRRKRRLSGKDADPPFDAAAAFDPALPFAGDADSVPS
                     SQAVQLVENLERAKTKELWRILVSLSIRHVGPVAARALASYFGSLDAIRSATRDELAA
                     VEGVGGIIADAVLDWFEVDWHREIIERWADAGVQFTTPGHPGPGAALALDGPLAGLTV
                     VATGSLDGFTREGAVEAIIAAGGKSASSVSKKTDFVAAGPGAGSKLTKAEELGVRIID
                     AQQFALLLAEGPAALE"
BASE COUNT          763 a         1554 c         1620 g          729 t
ORIGIN      
        1 cggaggccct tgagcgactc ggcccgggca ccggcgcgag cggcctcggc gaacgacggc
       61 cagacatcct tcaggctggt gctgtcgcgc ggatcgtgac cgccgatcac atcgtgcagc
      121 agaccggcat cgagcaccgt gcgcgtgacg ggtccgacct gatcgagcga ggacgcgaga
      181 gcgatcgccc cgtagcgcga cacaccaccg taggtgggct tgacgccgac cgaaccggtg
      241 accgcggcgg gctggcggat cgaaccaccc gtgtctgagc cgagcgccag cggcgcctcg
      301 aacgcggcga ccgcagcggc cgaaccacca ccggacccac cggggatgcg agtgaggtcc
      361 cacgggttct tcgtgggccc gtacgcggag tgctcggtgg acgagcccat cgcgaactcg
      421 tccatgttcg tcttgccgag cggtacgagc ccggcggcac gcagacgggc gacggtggtg
      481 gcgtcgtagg gcggaatcca gccctcgaga atcttggagc ccgcggtcga cggcatgtcg
      541 agcgtgcaga gcacatcctt gatcgcgacc ggcacaccgg cgagtggccc gagcgtgtcg
      601 ccggcggcgc gctgctggtc gatgcgctcg gcggtggcga gggcatccgc tgagatatgg
      661 aggaacgcgt gcacgtcgcc gtcgacggca gcgatgcggt cgaggtgcgc ctgcgtggcc
      721 tcgaccgagg agacctcacc cgaggcgagc ttgcttgcga ggtcggcggc gctgagacgg
      781 atcagctcag atgcggtcgc cccggcaccg gatgacgcgg cggcgctcac tgctcctccc
      841 ccagaattgc gctcacctgg aaccgactgc cgtcgtgctc gggagctccg gcgagcgcct
      901 gctcggtcgt gagcgtcacg ccgggaacgt cgtcgcggta gacgttgacg agagggatgg
      961 ggtggctggt cgccgggacg tctggcgtcg cgacttcgct gactttcgcc accgagtcga
     1021 cgatcgcacc gagctcgccg gtgagggtct ggatctcctc gtcggtcagc gctatgcgcg
     1081 cgagggaggc gagatgggtc acgacgtcgg gggtgatttc agacaccggc caattctacg
     1141 gggcgctctc gcgccgaggt cacactggca ccgcgctctc gcgccgggag tcacactgac
     1201 ggctcagccc tcagccgaag ccacacaggc gctctcaccc tgaatagtca tcgcttggtc
     1261 gctcgcccgg aagatcaggt actccccgtc atcgccgcga gcctgcacct cgaccacgtc
     1321 cgggccgtca agcgtgggcg tctgcgtcac ctcgtagccg agagcgctcc actgttcggc
     1381 aactgctcgt gcggcatcgg ccggcccagt cggcgacgta ccggccgcga ccctgagcgc
     1441 gggaacctgc agccccgcaa cgccgtggga attggcgcag gctcgcgaat ccgggtcgtc
     1501 ccgcacgtcc cagtcgccac ccaccagttc ctgggtcgtg acgaggactc tggcgaagtc
     1561 gcggtgcacc tcgtccgccg tcgcggccgc aacagcgcac ccggtgaggg atgccgcggc
     1621 gcacgccagt gcaaccacca tccccgctcg tgccagcctc ccccggccgg ccatgcctgc
     1681 gcgcatctgc aacccccatt gccagcggca aggttagccc ggccgggctg cacgagggca
     1741 tgcccagtgc ggggggcgac ggccgtcgac ggcgcaacgc tgcgcccggc gaattccccg
     1801 ctactgcggc gtgacgtcgt cgccgtgacc gatgccgatg agcgcaaggt tgcgcagcga
     1861 ctcggtcgcg ggactgaagt actcgatgtg gaagaacgac ctcaacatcg tctggcccgt
     1921 caacgggtcg gtaccaccat cggttccgaa ggtcgtcgca ccgtaggacg ccgagcccgg
     1981 ctcgctgccg aagaaggcgc tgttgggcac cggatcccac tgagccttcc ccacgaagac
     2041 gttgccgttc ttcacgtgca actgacgcac cgactgcgcg gcgctgcccg gcgatccgac
     2101 gacagccagc gcatccacct cgaagtcgta ctcggtgagt gccatcagcg cagcggtcga
     2161 cccataggag tgcccaacga tcgtgacgta gggctccgat gccgatctca gcgtctggag
     2221 tccctcgatc gagcgggcaa gcgagtcccg accctcttcg gcgttctcga ggctgcccac
     2281 attggtgagg ttgggggtgt ggtagccgat ccacgccacg gtcgcgaccg tgctcgtggt
     2341 gtctccgtag tgtgccagcc acgactgctg ctcgtcgcgc aggcgggccg cggcatccgt
     2401 ccaatagacc atctggccct cgatggtgaa gaacatgccg ggcaccatgt aggtgatgta
     2461 gtcggcggtc gccaggtcac cgacgatgat ggccgcacgc ccctggcctg tgacgtccag
     2521 gctcatgagc gtgcggttgg tgtttccgga tgcgtcaccc agcgcattcg agatcgactt
     2581 gagcatcgac agttggagct tcgccttctc gacggcggtg cgaccgagct ccgtgcggat
     2641 cgtgtagtcc agcccacgca tggtggacgt gagcaccgag cggttggcga gatcgcgcac
     2701 ctggtagggg atgccgtcga gatttccgac gagttgcggg gtggcaactc gcagggcgtt
     2761 gcgggcgtcg aggcccattg atccccacca cagcgcgacg tcctgcgacc gcggcgggtt
     2821 gccgatgagt gaggcgatgg cttcgggatt gtcgcgaacg aactggtcga tctggccgac
     2881 cggcagcaga gcgatctggc cgagaagctg ggtgcccttg acgacctcgg cgggggaacc
     2941 gatcgctccg tcgcgacccg tgggtgcggt gcccagcgtg gtggcgccac ccgactcggg
     3001 cacgaccgga ccgatgaacg gctgcggcgg gagcccgggg agctccgaac ccacgaggtc
     3061 ggagcccgcg ccatcgggtg ccgcgagggt gatcgtggtg agcgagagcg gtgccggcac
     3121 gttctcctga gtggagatgg atgtcgcgta ccgggcgatt tcgtgcacac cgaaggcctc
     3181 ggcgacggaa cccagtgccg ccgaggttgt tagaacagct gtgacgtcaa acagcaattc
     3241 gggccaatcc cctcgcgcaa cgatgtggcc cgcccacccc agatcacgtc attgcacccc
     3301 ccatcgtagg ggatagaacg ccccgtatgg gggacaagtg tgttaagggt ggggaaattg
     3361 ttcggaaaac gacaaccctt cagaacgcag cattcgtgcg aaaagtgctg gtgattgagg
     3421 cattgtgctt cgaaatgcgc gattgcggcg tcaacacgca gagactttgc gccgcgaccg
     3481 gggtgcaccg tgcggtgggt agaacggtac gactaccggg atgggggtca gctcacgacc
     3541 tagattagat gggaaagccg agcttgtttc cgagatgcgg ggcggccccc tatgtgcccc
     3601 gactaacagc cgatcactcc agcgcggctg gcccctccgc gagcagcagc gcgaattgct
     3661 gagcgtcgat gatgcgaacg cccaactcct ccgccttcgt cagcttcgac ccggcccccg
     3721 gaccggccgc gacgaagtcg gtcttcttgg agacacttga ggctgacttg cccccggccg
     3781 cgatgatcgc ctcgactgca ccctcgcgag tgaacccgtc gagactgccg gtcgcgacga
     3841 cggtgagtcc ggcgagtggt ccatcgaggg cgagtgctgc ccccggcccg gggtgaccgg
     3901 gcgtcgtgaa ctgcacgccg gcatccgccc agcgttcgat gatctcgcga tgccagtcga
     3961 cctcgaacca gtcgagcacc gcgtcagcga tgatgccacc gacgccctcg acggctgcga
     4021 gctcatcgcg ggtcgcggag cggatcgcgt cgagcgaccc gaagtagctg gcgagggcac
     4081 gagccgccac cggtccgacg tgacggatcg acagcgagac gaggatgcgc cacaactcct
     4141 tggtcttcgc ccgctcgagg ttctcgacca gctgcacggc ctggctggac ggcaccgagt
     4201 cggcgtcgcc cgcgaatggc agagcggggt cgaacgcggc ggccgcatcg aacggcgggt
     4261 cggcatcctt gccgctgagc cgacgtttgc gacggaacgg agtctcggtc ttcacccgca
     4321 gcgtgactcc gtcaccggtc gtgatctggg ttcctggttc gtcgtcgccc acctgtcgag
     4381 gcagaccggt ctcgttgtca cggacgacca cggtgatggg gaagagctgc tccagcgtga
     4441 gcgagaacag cccggcctcg gtgaccagcg gtggcgtcgc cggcacgagc ggctgcgtga
     4501 gcgcagcagc cgacacctcg ccgagccctt cgatgtcgag ggcaccccga ctgccgacgt
     4561 gctcgacgcg accccgcacc tgggcgggac agctgcgcgc attggggcaa cgcaggtcga
     4621 tgtccccctc cttggcgggg gcgagggcgg tgccgcactc cgggca
//
LOCUS       DAOSOU010000173         4645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18541_length_4645_cov_5.402614, whole genome shotgun sequence.
ACCESSION   DAOSOU010000173 DAOSOU010000000
VERSION     DAOSOU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18541_length_4645_cov_5.402614"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1136)
                     /gene="mraY"
                     /locus_tag="PLW15_09150"
     CDS             complement(24..1136)
                     /gene="mraY"
                     /locus_tag="PLW15_09150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT34597.1"
                     /translation="MIALLIAGGVSLAFTLFLTPLFIRLFTRLKWGQFIRDDGPQSHH
                     TKRGTPTMGGIIFIIGATTGYFVGHAVAGEPLTLSGLLVIYLMVGLGIIGFIDDFLKT
                     RKQRSLGLGGWSKIAGQVIVAGGFAALALNFPDANGLTPASSMISAVRDIHWLNLAVF
                     GVVLGGIAFAIWVTLIIVSASNGVNVADGLDGLATGASIFAISSYIFIGFWQFNQNCT
                     NPTIDPDVLYKCYEIRDPLDLAVIAAAITGALIGFLWWNTSPAQIFMGDSGSLGLGGA
                     LAALAILSRTELLLVLIGGLFLVVTGSVILQRTWFKITKWRYGQGRRIFLMSPLHHHF
                     ELKGWAEITVVVRFWLISALFVAVGVGFFYLEWINQ"
     gene            complement(1133..2533)
                     /gene="murF"
                     /locus_tag="PLW15_09155"
     CDS             complement(1133..2533)
                     /gene="murF"
                     /locus_tag="PLW15_09155"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT34598.1"
                     /translation="MIELSPTEIAQIVGGRLVAGPDAPSTVSGEVHTDSRKVVPGSIF
                     FALPGEVTDGHLFVANAVENGAALLIVERELDSPAAQIVVADGYDALASLAREVVARV
                     RARGTLRVVAVTGSNGKTTTKNILRAVLEGEGPTVAPWGSFNNHVGAPISILAVDYST
                     RYLVVEMGASAIGEISRLISIVTPDVGIVLKVGLAHVGEFGGPDAVETAKSEMVTDLP
                     EGAVAILNADDDRVVRMAAKTRARVEWFGLDHTADVRATDVAATASGTAFTLEVDGVT
                     RRVQLRILGEHHVMNALAAIAATRALGVPVERSVPALEALERAERWRMEVLTRADGVV
                     VINDAYNASPDSMAAALKTLAQITAGDQRSVAVLGEMAELGEYADEEHDRIGRLAVRL
                     NVQKLIVVGHNARHIHNAAGLEGSWDGESVLVATADEAYDVLRDELRSGDVVLVKSSG
                     SAELRFLGDRLAGVTS"
     gene            complement(2530..4074)
                     /locus_tag="PLW15_09160"
     CDS             complement(2530..4074)
                     /locus_tag="PLW15_09160"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT34599.1"
                     /translation="MGSGEGSILRPQHPSARHLSALVDEFGLETRGSLDDVEVTGITL
                     STDNLQPGDLYVGVPGANRHGATFAAQAKEGGAVALLTDAAGADLAADSGLPMIIVES
                     PREALGAISAWVYRTAQAAPLLFGITGTNGKTSVSYLLHGMLAQLGLVAGLSTTAERK
                     IGDLTVASSLTTPEATEVHGLLARMRESEVRAVVLEVSAQALTRNRVDGLVFDVVGFL
                     NLSHDHLDDYADMETYFAAKLPLFEPDRARRGVVSLDTEWGQRVVDASRIPITTVSST
                     PGVDAAWTVTILEERAEYTEFELTPPEGQSITTRVPVIGRHMAANAGLAIAMLVEAGF
                     ELEAIAHALDQDGGIKAFIPGRIERVSGPSGPAVYVDYGHSPDAFLTTMDAVRRFTTG
                     RLIMVFGADGDRDASKRLDMGRIGSEGSDVLVITDYNPRFEDAAQIRAILLEGATSAV
                     NPAEIHEVPSQAQAIRVAIGMAREGDTVLWAGPGHEDSIDVQGEKLPFSARDEARRAL
                     REAGWA"
     gene            complement(4101..>4645)
                     /locus_tag="PLW15_09165"
     CDS             complement(4101..>4645)
                     /locus_tag="PLW15_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT34600.1"
                     /translation="NEWDEVTNYAVQFGQGMSATSVQVASAYQTLANGGVRMPVTLVE
                     GCQWPDGTTTDLPATEGTRVVSETAARQTSEMLEQVVTKGWKSPDLTIPGYRVAAKSG
                     TAQVAEDGIYGDKAVISYAGFAPADNPQYVVVVTAGIPSSIYSSGAIATTFHDVMAQT
                     LTQFRVTPSTEPAPDIPLTW"
BASE COUNT          830 a         1535 c         1534 g          746 t
ORIGIN      
        1 cagctcgtcg agatcccgca cggctactgg ttgatccatt cgaggtagaa gaacccgacg
       61 cccactgcga cgaacaacgc cgagatgagc cagaaccgca cgacgacggt gatctcggcc
      121 cagcccttga gttcgaaatg gtgatggagg gggctcatga ggaaaatgcg tctgccctgc
      181 ccgtagcgcc acttggtgat cttgaaccag gtgcgctgca ggatgaccga ccccgtcacg
      241 acgaggaaca ggccgccgat gagcacgagg agcaactcgg tgcggctgag gatggcgagc
      301 gcggccagcg cgccgccgag gccgagcgaa ccggagtcgc ccatgaagat ctgggcgggc
      361 gaggtgttcc accacaggaa cccgatgagc gcaccggtga tggcggccgc gatgaccgcg
      421 aggtcgagcg ggtcgcggat ctcgtagcac ttgtagagca cgtcgggatc gatcgtcggg
      481 ttggtgcagt tctgattgaa ctgccagaac ccgatgaaga tgtacgacga gatcgcgaag
      541 atcgaggctc cggttgcaag cccgtcgagc ccgtcggcga cattgacgcc gttcgacgcg
      601 ctcacgatga tcagcgtcac ccagatggcg aaggcgatcc cgcccagaac cacgccgaag
      661 acggcgagat tgagccagtg gatgtcacgc accgccgaga tcatcgacga tgcgggcgta
      721 agcccgttcg cgtcggggaa gttcagggcg agcgcggcga acccacctgc cacgatgacc
      781 tgcccggcga tcttcgacca gcccccgagc cccaggctgc gctgtttgcg ggtcttgagg
      841 aagtcgtcga tgaacccgat gatgccgagg ccgaccatga ggtagatgac cagcagtcca
      901 gacagtgtga gaggctcacc ggccacggca tggccgacga agtagccggt cgtcgcaccg
      961 atgatgaaga tgatgccacc catggtggga gtgccacgct tggtgtggtg agactgcgga
     1021 ccgtcatcac ggatgaactg gccccacttg aggcgagtga agaggcggat gaacaacggt
     1081 gtcaggaaca gcgtgaacgc cagcgagacc ccgccggcga tgagcagcgc gatcacgagg
     1141 tgactcccgc gagacgatca ccgaggaatc gcagttcggc agatcctgac gacttcacga
     1201 gcaccacgtc cccactgcgc aactcgtcac gcagcacatc gtaagcctca tcagcggtgg
     1261 caacgagcac cgactccccg tcccacgacc cctcgaggcc tgcggcgttg tggatgtggc
     1321 gggcattgtg ccccaccacg atcagtttct gcacgttgag ccgaaccgcc agtcgcccga
     1381 tgcgatcgtg ctcctcgtcg gcgtactcac cgagttcggc catctcaccg agcaccgcca
     1441 ccgaccgctg gtcgccggca gtgatctggg cgagggtctt gagggccgct gccatcgaat
     1501 cgggactcgc gttgtacgcg tcgttgatca cgacgacccc gtcggcgcgg gtgagcactt
     1561 ccatccgcca tcgctcggcg cgctcgagag cctcaagggc gggcaccgac cgttcgacgg
     1621 gaacgccgag cgcccgcgtg gcagcgatgg ccgcaagggc attcatgacg tggtgctcac
     1681 cgaggatccg cagctgcacg cgacgggtca ccccatcgac ctcgagagtg aaggcggtgc
     1741 cggatgccgt cgccgcgaca tccgtcgccc gaacgtcggc cgtgtggtcc agaccgaacc
     1801 actcaacccg agcgcgggtc ttcgccgcca tacgcaccac ccggtcgtcg tcggcgttga
     1861 ggatggcgac agcgccctcg ggaaggtcgg tgaccatctc ggacttggcc gtctccacgg
     1921 cgtccggccc gccgaactcg cccacatggg cgaggcccac cttcagcacg attccgacat
     1981 ccggggtcac tatcgagatc aggcgggaga tctccccgat cgcactggcc cccatctcga
     2041 cgacgagata gcgggtcgag tagtcgacgg cgaggatcga gatgggcgcg ccgacgtgat
     2101 tgttgaacga accccacggc gcgacggtcg gcccctcgcc ctcgagcacg gcccggagaa
     2161 tgttcttggt ggtcgtcttg ccgttcgagc ccgtcacagc cacaacgcgg agggtgcccc
     2221 gggcgcgaac ccgcgcgacc acctctctgg ccagcgacgc gagagcgtca tagccgtcgg
     2281 cgacgacgat ctgggccgcg gggctgtcca gctcgcgctc gacgatcagc agcgctgcgc
     2341 cattctcgac cgcgttcgct acgaagaggt gaccgtcggt gacctccccc ggaagcgcga
     2401 agaagatcga ccccggcacg accttgcggg aatcggtgtg cacctcgccg gacacggtgc
     2461 tcggggcatc cgggccagct accaggcgac ctccgacgat ctgagcgatc tcggtcggac
     2521 tcagttcgat catgcccagc ccgcttcacg cagggcgcgt cgcgcttcgt ctcgcgccga
     2581 gaacggaagc ttctcgccct gaacatcgat cgagtcctcg tgaccgggac ctgcccacag
     2641 cacagtgtca ccctcgcgcg ccatgccgat cgccacccgg atcgcctgag cctgagacgg
     2701 aacctcgtgg atctccgcag ggttcacggc cgacgtggcg ccctccagga ggatcgccct
     2761 gatctgggcg gcatcctcga atcgagggtt gtagtcggtg atgacgagca cgtcagatcc
     2821 ttccgagccg attcggccca tgtcgagtcg cttcgaggcg tcgcggtcac cgtcggcgcc
     2881 gaagaccatg atgagccggc cggtggtgaa tcgccgcacc gcatccatcg tcgtcaggaa
     2941 ggcgtccggg ctgtgaccgt agtcgacata cacggcgggc ccggatggac cggagacccg
     3001 ctcgatgcgt ccggggatga acgccttgat tcccccgtcc tggtcgaggg cgtgagcgat
     3061 cgcctcgagt tcgaaaccgg cttcgacgag catcgcgatc gccagccctg cgttggccgc
     3121 catgtggcga ccgatcaccg gtacccgagt ggtgatcgat tggccctcgg gaggcgtcag
     3181 ctcgaactcc gtgtactcgg cgcgctcctc aaggatggtg acggtccagg ccgcgtcgac
     3241 cccgggagtc gacgacactg tcgtgatggg gatgcgagaa gcgtcaacga cgcgctgacc
     3301 ccactcggtg tcgagcgaga ccaccccgcg acgagcgcga tcgggttcga acagcgggag
     3361 cttcgccgcg aaataggtct ccatgtcggc atagtcgtcg aggtgatcgt gactgagatt
     3421 gaggaaaccc accacgtcga agacgagtcc gtcgacccgg ttgcgggtga gcgcctgcgc
     3481 gctgacctcc agaaccaccg cgcgcacctc gctctcgcgc atgcgggcga ggagcccgtg
     3541 cacctcggtc gcctccgggg tcgtcaggct gctggcgacg gtgaggtcgc cgatcttccg
     3601 ctcggccgtc gtggacaggc cggctacgag cccgagctga gcgagcatcc catggagcag
     3661 gtaggacaca ctggtcttgc cattggtacc cgtgatcccg aacaggagcg gtgccgcctg
     3721 ggcagtgcgg tagacccacg ccgagatggc acccagggcc tcgcggggcg actccacgat
     3781 gatcatgggc agaccgctgt cagcggcaag gtcggccccc gccgcgtcgg tgagaagcgc
     3841 gaccgcgccg ccctccttcg cctgggccgc gaacgtggct ccgtgccgat tcgcgccggg
     3901 tactccgacg tagaggtcgc cgggctgcag attgtcagta ctgagggtga tgccggtcac
     3961 ctcgacgtcg tcgagcgatc ccctggtctc caggccgaat tcgtcgacaa gtgccgacag
     4021 atgacgggca gagggatgct gggggcgaag aatcgagcct tcaccggaac ccactcgatc
     4081 tcccgctctg tactagtgca tcaccacgtc agtgggatgt ccggggccgg ttccgttgat
     4141 ggtgtgacac ggaactgggt cagcgtctgc gccatcacat cgtggaacgt cgtcgcgatt
     4201 gcccctgagg aatagatgct actagggatg cccgccgtca cgacaaccac gtactgcggg
     4261 ttgtccgccg gagcgaagcc cgcgtaggag atcacggcct tgtcgccgta gatgccgtcc
     4321 tcggcaacct gcgccgtacc actcttggcg gcgacccggt atccggggat ggtcaggtcg
     4381 ggtgacttcc accccttggt gacgacctgc tccaacatct ccgacgtctg acgggccgcg
     4441 gtctccgata ccacgcgggt gccttccgtc gccggcaggt ctgttgtggt gccgtcgggc
     4501 cactggcatc cctcgacgag cgtcacgggc atcctgacgc cgccgttggc cagagtctgg
     4561 tacgcgctgg cgacctgcac cgaggtcgcg ctcatgccct ggccgaactg caccgcgtag
     4621 ttggtgacct cgtcccactc gttgg
//
LOCUS       DAOSOU010000174         4622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18664_length_4622_cov_6.380118, whole genome shotgun sequence.
ACCESSION   DAOSOU010000174 DAOSOU010000000
VERSION     DAOSOU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18664_length_4622_cov_6.380118"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLW15_09170"
     CDS             <1..709
                     /locus_tag="PLW15_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT34601.1"
                     /translation="GPVEVLKGVNLRAYAGKVTALVGDNGAGKSTLVKGLAGVQPYDS
                     GEVRFAGEIVHLHTPREAAALGIEVVYQDLALCENLDIVQNMFLGREETSAGTLDEGV
                     MERQASETLQSLSVRTVKSVRQKVSSLSGGQRQTVAIARSVLKNAKVVILDEPTAALG
                     VAQTEQVLHLVRRLADSGVAVIIISHNLADVFAVADYISVLYLGQMVADLETKNTNND
                     DVVGYITGSKTYEGTLA"
     gene            706..1983
                     /locus_tag="PLW15_09175"
     CDS             706..1983
                     /locus_tag="PLW15_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT34602.1"
                     /translation="MSTPVSPPPVIGSGYEGGIGDQVVNYFRRVRQGEMGALPALGGF
                     VVLIILFSFLSGNFFTPLNLANLLTQTATLMTLAMALVFVILLGEIDLSAGVTSGVTA
                     SVFVVLVHNIQMNWILALLIAFAVGMLIGTFIGFFVARVGIPSFVVTLGLFLAIPGLQ
                     LLIIGTGGAYRIETPEISAIMNKNLPIVTGWVLLGVAIAIDILISLWDRARRRRAGVL
                     NRPLTLMWARIGALAVGGALFVALMSVDRAAGRGSLNGVPIAHDAIEGVPIVVPIAVG
                     LLWVGTFILDRTRYGRHLYAVGGNPEAARRAGIKVAGIKMSAFIVCSTLAVVAGLFDA
                     SRIRVVTASSGSQIVLSGVAAAVVGGVSLFGGRGRLIHAAIGALVIAVISNGLGLLGL
                     PAGVNFLVTGGVLILAATVDAISRKRSGATLLR"
     gene            complement(2028..2996)
                     /locus_tag="PLW15_09180"
     CDS             complement(2028..2996)
                     /locus_tag="PLW15_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT34603.1"
                     /translation="MPRTDLTLDELRTYRSAAEVPADFDEFWDRTLAESRAAGGDVIL
                     VPAESPVSELVVEDLTFAGFGGEPIRGWVIRPRDSQPRATVVQYVGYGGGRGLAHEHL
                     QWASSGFVHVVMDTRGQGSAWGTGGDTPDPHGSGPAFPGFMTRGIQSRETYYYRRVFT
                     DAVRLIDAVRGFDFVDPIAVAVTGGSQGGAISLAVAGLVPGLRAVMPDVPFLCDFRRS
                     VELTPDAPFTELRNYLAIHRDVVDETFTTLAYFDGVNFARRATAPARFSVALMDPIVL
                     PSTVFAAYNDYAGDDREIDVYPYNGHEGGQGHQWVTQARWLAGRSR"
     gene            complement(3028..4401)
                     /gene="xylB"
                     /locus_tag="PLW15_09185"
     CDS             complement(3028..4401)
                     /gene="xylB"
                     /locus_tag="PLW15_09185"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT34604.1"
                     /translation="MTLVAGVDSSTQSCKVVVVDAATGALVREGRAAHPAGTEVDPEA
                     WWDALQTAIAAAGGLDDVSAVSVGGQQHGMVALDGQGRVIRPALLWNDTRSAQAAADL
                     TAEVPDIAERTGSVPVASFTSTKLRWLRDAEPENAERVAAVALPHDWLTWRLRGFGPE
                     NPRLDELTTDRSDASGTGYFNSVTGEYDLELLERALGHSAILPRVLGPHESAGTVGDL
                     VVGPGAGDNAGAALGLGAVAGDVIVSIGTSGTVFAVTGSAARDATGAVAGFADASGLF
                     LPLVATLNAARILDSIAGLLGVDHTGLGELALAAAPGAGGLVLQPYFEGERTPNLPDA
                     TATLFGMTLASTTRNNLARAAIEGLLCGLADGLDAVRRTGVVAERILLIGGAAQNPAV
                     SAIAAQVFDCPVVVPNPGEYVALGAAVQAAWTLTGTRPAWEIPLVAAPAPDYHPVIRE
                     QYAAHLS"
BASE COUNT          711 a         1571 c         1596 g          744 t
ORIGIN      
        1 cggtccggtg gaagtgctca agggggtgaa cctccgcgct tatgccggca aggtcaccgc
       61 tctcgtcggc gacaacggcg ctggcaagtc caccctcgtg aagggcctcg ccggggtgca
      121 gccctacgac tcgggtgagg ttcgctttgc gggggagatc gttcacctgc acacgccccg
      181 cgaagctgcg gcactcggca tcgaggtcgt gtaccaggat ctcgcgctgt gcgagaacct
      241 cgacatcgtg cagaacatgt tcctcggccg cgaggagacg tcggccggca ccctcgacga
      301 gggcgtcatg gaacgccagg cctccgagac actccagtcg ctgtcggtgc gcacggtcaa
      361 gtccgttcgc cagaaggtgt cgtcgctgtc cggcggacag cgccagaccg tcgcgatcgc
      421 acgctccgtg ctcaagaacg ccaaggtggt catcctcgac gagcccaccg ccgcactcgg
      481 agtcgcgcag acggagcagg tgcttcacct cgtgcgtcgc ctcgccgact cgggtgtcgc
      541 ggtcatcatc atcagccaca acctggccga cgtcttcgcg gtcgctgact acatcagtgt
      601 gctgtacctc ggccagatgg tggccgatct cgaaaccaag aacaccaaca acgacgacgt
      661 tgtcggatac atcaccggca gcaagaccta cgaaggaacc ctcgcatgag cacaccggtc
      721 agcccgcccc cggtcatcgg cagtgggtat gaaggcggca tcggcgatca ggtcgtcaac
      781 tacttccgcc gcgtgcggca gggtgagatg ggcgcgcttc cggccctcgg cggtttcgtc
      841 gtgctcatca tcctgttctc cttcttgagc gggaacttct tcactccgct caacctcgcg
      901 aacctgctca cccagaccgc gacgctcatg accctggcga tggcgctggt cttcgtgatc
      961 ctgctcggtg agatcgacct gtctgcgggt gtgacctcgg gcgtgacggc gagcgtgttc
     1021 gtcgtgctcg tgcacaacat ccagatgaac tggattctcg cgctcctcat cgccttcgcg
     1081 gtgggaatgc tcatcggaac cttcatcgga ttcttcgtgg cgcgcgtcgg gatcccgtcg
     1141 ttcgtggtga ccctgggact cttcctggcg atccccggtc tccagctgct gatcatcgga
     1201 accggaggtg cgtaccgcat cgagacgccc gagatctcgg cgatcatgaa caagaacctg
     1261 cccatcgtca cgggctgggt tctgctggga gtcgcgattg cgatcgacat cctgatctcg
     1321 ctgtgggacc gtgcgcggcg tcggcgggcc ggcgtcctca atcgcccgct cactctgatg
     1381 tgggcgcgga tcggggctct ggcagtcggt ggcgcgctct tcgtcgctct catgagcgtg
     1441 gaccgcgcgg cgggccgtgg ctccctcaac ggcgtgccga tcgcgcacga cgccatcgag
     1501 ggagtgccga tcgtggttcc gatcgctgtc gggctgttgt gggtaggaac gttcatcctg
     1561 gatcgcacca ggtacggccg acacctctat gcggtgggtg gaaaccccga ggccgcgcgt
     1621 cgcgccggta tcaaggtcgc gggaatcaag atgtccgcgt tcatcgtctg ctcgactctc
     1681 gcggtggtgg ccggtctctt cgacgcgagc cgcatccgcg tggtgacggc atcctcggga
     1741 agccagatcg tgctgagcgg cgtcgctgct gctgtcgtcg gtggcgtgag cctcttcggt
     1801 ggccgtgggc gtctcatcca cgcggccatc ggcgccctcg tgatcgctgt gatctcgaac
     1861 gggctcggcc tgctcggcct cccggccggc gtcaacttcc tcgtgaccgg cggcgtcctg
     1921 attctcgccg cgaccgtgga tgccatctcg cgcaagcgct cgggagcgac cctgctgcgc
     1981 tagcggtcaa ctcccagcgt cagctcacat ggctggccgg gtctcgccta ccgcgagcgc
     2041 ccggccagcc atcgtgcttg tgtgacccat tgatggccct gcccgccctc gtgaccgttg
     2101 tacgggtaca cgtcgatctc gcggtcgtcg cccgcatagt cgttgtaggc ggcgaagacc
     2161 gtcgacggca gcacgatcgg gtccatgagg gcgaccgaga accgcgcggg tgctgtggcg
     2221 cggcgcgcga agttgacacc gtcgaagtag gccagcgtcg tgaacgtctc gtccacgacg
     2281 tcgcggtgga tggccaggta attgcgcaac tccgtgaagg gggcgtcggg ggtcagttcg
     2341 accgaccggc ggaaatcgca gaggaacggg acatccggca tcacggctcg caagccggga
     2401 accagtccgg cgacggcaag gctgatcgcg ccgccttggc tgccaccggt cacggccacg
     2461 gcgatcgggt cgacgaagtc gaacccgcgc accgcgtcga tgaggcgcac cgcgtcggtg
     2521 aacaccctgc ggtagtagta ggtctcacga gactggatgc cgcgggtcat gaacccggga
     2581 aacgccggcc ccgaaccgtg cgggtccgga gtgtcgccgc cggtgcccca ggcgctgccc
     2641 tgcccgcggg tgtccatgac cacgtgcacg aagccgctgg atgcccactg caggtgctcg
     2701 tgcgccagtc cgcgcccccc gccgtagccg acgtactgca cgaccgtcgc tcgcggctgc
     2761 gagtcgcgag gcctgataac ccagccgcgg atgggctcac ccccgaaccc cgcgaacgtg
     2821 aggtcctcga ccacgagttc actcacgggg gactcggcgg gcacaagtat gacgtcgcca
     2881 ccggccgcgc gtgactcggc gagggtgcga tcccagaact cgtcgaagtc ggcgggcacc
     2941 tcggccgctg atcggtaggt cctcagctcg tcgagcgtga gatcggtgcg cggcatggct
     3001 tctcctcggt tcttacccgg gaagagccta ggagaggtgg gctgcgtact gctcgcgaat
     3061 caccgggtgg tagtcgggag ccggcgccgc gacgagcggg atctcccagg ccgggcgcgt
     3121 gccggtcagc gtccacgcgg cctgcaccgc ggccccgagc gcgacatact cgcccggatt
     3181 cggaacgacg accgggcagt cgaacacctg ggcggcgatc gccgagacgg ccgggttctg
     3241 tgcagcgccc ccgatgagca ggatgcgctc ggcgacgacg cccgtgcgcc gcaccgcgtc
     3301 gagtccgtcg gcgagtccgc acagcaggcc ctcgatggcg gcgcgagcga ggttgttgcg
     3361 ggtggtggag gccagggtca tcccgaagag tgtggccgtg gcatccggaa ggttgggggt
     3421 gcgctcgccc tcgaagtagg gctggagcac gaggccgccg gcgccgggcg cggcagccag
     3481 cgcgagctcg cccagcccgg tgtggtcgac gcccagcagc ccggcaatcg agtcgaggat
     3541 gcgggcggcg ttgagcgtcg ccaccagcgg aaggaacagt ccgctggcgt cggcgaagcc
     3601 cgccacggcg cctgtcgcgt cgcgggcggc cgaaccggtg accgcgaaga ccgtgccgct
     3661 ggtgccgatc gacacgatca catcaccggc caccgcgccg agccccagcg cggcaccggc
     3721 attgtcgccg gcaccgggac cgacgacgag gtcaccgacc gtgcccgccg actcgtgcgg
     3781 gccgagcacc cggggaagga tcgccgagtg gccgagggcg cgctccagca gctccaggtc
     3841 gtactcaccg gtgacggagt tgaagtagcc ggtgccgctc gcgtcggatc ggtcggtcgt
     3901 gagctcgtcg agccgcgggt tctcgggccc gaaaccgcgc agccgccagg tcagccagtc
     3961 gtgcgggagg gcgacggccg caacccgctc ggcgttctcc ggctcggcgt cgcgcagcca
     4021 gcgcagcttc gtcgacgtaa aactcgcgac cggcaccgac ccggtgcgct cggcgatgtc
     4081 gggaacctcc gccgtcaggt ctgccgcagc ctgcgccgag cgggtgtcgt tccagagcag
     4141 ggcgggccgg atgacgcgtc cctgtccatc gagggcgacc atgccgtgct gctgcccgcc
     4201 aacggacaca gcactcacgt cgtcgagtcc cccggccgcc gcgatcgcgg tctggagggc
     4261 atcccaccag gcctcggggt cgacctcggt gccggcgggg tgagccgcgc ggccctcgcg
     4321 cacgagggcg ccggtggcgg catccacgac gacgaccttg cacgactggg tcgacgaatc
     4381 gacccctgct accaacgtca tcgcccgccc tccccttccg ccggttcata actcctgcag
     4441 agcgaccggc tggagggccg acacgccgcg aaatcgaccg cgaggtcgga ttgtgcagga
     4501 gttatgaaca ccgggtgggg gagggagtgc gaaacggcga tcatcagcta gcgcgcgccc
     4561 atgaggtgct cggtcgccag ctgctgcagg cgcacgaagc cgaagccctt gccgttgaag
     4621 ta
//
LOCUS       DAOSOU010000175         4611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18730_length_4611_cov_4.973881, whole genome shotgun sequence.
ACCESSION   DAOSOU010000175 DAOSOU010000000
VERSION     DAOSOU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18730_length_4611_cov_4.973881"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLW15_09190"
     CDS             complement(<1..654)
                     /locus_tag="PLW15_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT34605.1"
                     /translation="MSPTLADVAAVVERLWPASGAEPWDAVGLIAGDPAAHVDSILLA
                     VDAVHDTVDEAVASGVDLLLVHHPLLLRGVTSVAADRYKGAALTRLIRAEIALMAAHT
                     NADVVESGTSATLASVLGLVDIVPLDPGSDPGRGIGRLGRLPAPMTLGRLARTLGEIL
                     PATATGIRVAGDFDAPVTTVALCAGAGDSLLGHPAVLEADVYITSDLRHHPASEAREN
                     "
     gene            complement(699..3143)
                     /locus_tag="PLW15_09195"
     CDS             complement(699..3143)
                     /locus_tag="PLW15_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOT34606.1"
                     /translation="MRDPELLSLLEQAGLETAGGTLSAIEQLEAQLSARGIPVGPTEQ
                     PIDVPPADVAPVSLAESFVVEDQPVAAWPAPQTDDAPRVNDTVPVKPVEPVVLEPVVE
                     PEPVVAAPPPLPAWDVPPPSAGWASTLSEPEPEPEPEPEPAPEPELVVEPEPLPEPEP
                     VFEPQPPLVSGPPPLVEPPILGGPPLLPSQAAGHPPIPIVPIPEYEEVIEAEVIEDEP
                     SAAPPPPPPAFSFDDLLSGPADDDLRPEVDEGRPFAAETLFEEPAPVTASQGVPLDTD
                     SIAVIDRAYEEELDDDVDDTDRAFAGLVGGAAIVSPPSGPISTVRIPEDEVVLIDNEP
                     VRQRVFSVETSGLEPTPVDHRIGRASRLFWLWFAANSSILSLGLGAAVFAVGMSLRQS
                     IVSVLAGVALSFIPLGLSTLAGKRSGQPTMVVSRATFGVVGNVVPALLALVTRVFWGA
                     VLLWLLASSVAVVLAGAQLDGGLGERLLLVLGLAAGLVIAMLVAVVGYHLIAQIQLVL
                     SVVSGILIIGLIAMTAQYVDVPAALTTPDGPWLLTITGAVLVFSFVGLVWANSGADLA
                     RYQRPGSSGAASMLWSTIGATLPSFVLIGYGALLAASDKGIASGFVQSPLDTLALMLP
                     SWYPAPLIAATVLSLLSGIVITLYSGGFALQSVGVRVSRQWSVVIVGVLLAAIAVVLS
                     FAVTGIMELFRDLATTLAVPTAAWAGIFAAEMMIRNRRFESESLLRRGGVYETVRWGN
                     LIGFVVISAIGWGLTTATVTWLGWQGYLFGLLGVPLDTDLAGTDLGVLVALALGILLP
                     IIIGFPAIRRQEEARP"
     gene            complement(3540..4457)
                     /locus_tag="PLW15_09200"
     CDS             complement(3540..4457)
                     /locus_tag="PLW15_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT34607.1"
                     /translation="MRGIPAASSSGVWALILASMLWGTTGTAASFLPDSVSPLAVGAS
                     TMTIGGLLLFLVSARPALAAVRASSSRRWLLIGAVGVFVYPLAFYSSMSLAGVAIGNV
                     VSLGTGPVFAALLEWIVERRRLTLRWALCTAVAIVGVILLALGSHRDDAPQATSPLLG
                     IGLGVAAGLAYALYTYASSRAIGTGQPSRAVMGSMFGLGAIALLPVLLVFGAPLLQST
                     TTVGIAAYLALGPMFVAYLLFGVGMRTLRSSTATTITLLEPVVATILAVLVVGERLEL
                     LGWAGLILILGAVTVLATARQPRSPGQSP"
BASE COUNT          842 a         1547 c         1604 g          618 t
ORIGIN      
        1 gttctcgcgt gcctccgatg ccgggtggtg acgcaggtcg ctcgtgatgt acacgtcggc
       61 ctcgagaacg gcggggtggc ccagaagcga gtcaccagcg cctgcgcaca gcgccactgt
      121 cgtcacggga gcgtcgaagt caccggccac tcggatgccc gttgcggtgg cgggcagaat
      181 ctcgccgagg gtgcgggcga ggcgccccag agtcatgggc gcgggcaggc gtccgagccg
      241 gccgatgcct cgcccggggt cagagcccgg atcgagcggg acgatgtcga caaggcccaa
      301 gaccgatgcc agagtcgccg acgttccgct ctcgaccacg tcggcattgg tgtgggcggc
      361 catcagcgcg atctccgcgc ggatcaagcg cgtgagcgcc gcgcctttgt agcgatccgc
      421 ggcaaccgag gtcacgcctc gcagtagcag ggggtggtgc acgagcagca ggtccactcc
      481 cgaggcgacc gcctcgtcga ccgtgtcatg cactgcgtcc acggcgagga ggatcgagtc
      541 cacgtgcgcg gccgggtctc cggcgatgag cccgactgca tcccatggtt ccgcaccgct
      601 tgcgggccac agtcgttcga cgactgcggc gacatcggca agtgtcggtg acatgccccc
      661 agtctatttg cgcgggtgag cgccgtgagg cgtgccggtc agggccgcgc ctcctcctgg
      721 cggcggatgg ccgggaatcc gatgatgatc ggcagcagga tgcccagcgc tagagcgacg
      781 agaaccccga gatcggttcc cgcgaggtcg gtgtccagcg gaactccgag cagcccgaac
      841 aggtagccct gccatcccag ccaggtgacg gtcgccgtcg tgaggcccca tccgatggca
      901 ctgatcacga cgaagccgat gagattgccc cacctgacgg tctcgtagac tcctccgcgc
      961 cgaagcagcg attcgctctc gaagcgacga ttgcggatca tcatctcggc cgcgaagatg
     1021 ccggcccagg ccgcagtcgg aacggccaga gttgtggcga gatccctgaa cagctccatg
     1081 atgccggtca ccgcgaagct gagcaccacg gcgatcgccg ccaggagcac tcccacgatc
     1141 acgaccgacc actggcggga gacccgcacg ccgacagact ggagcgcgaa tccgcccgag
     1201 tagagcgtga tcacgatgcc cgagagcagg ctgagcaccg tcgcggcgat caacggagcg
     1261 gggtaccacg acggcagcat gagcgcgagg gtatccagcg gcgactgaac gaacccactc
     1321 gcgatcccct tgtcggatgc cgcgagcagg gcaccgtacc cgatgagcac gaaactgggc
     1381 agcgttgcac cgatcgtcga ccacagcatg gatgccgcgc cgctcgaccc cgggcgctgg
     1441 taacgcgcca gatccgcgcc gctgttggcc cacacgagtc ccacgaagct gaacaccagc
     1501 acggcaccgg tgatcgtcag cagccacggg ccgtcgggcg tcgtgagcgc cgcgggcaca
     1561 tccacgtact gcgcggtcat ggcgatgagg ccgatgatca ggatgccgga caccacgctc
     1621 agcacgagct ggatctgcgc gatcaggtga tagccgacga cggcgacgag catggcgatg
     1681 accagtccgg ccgcgaggcc gagaaccagg agcagacgct caccgaggcc gccgtctagt
     1741 tgggctcctg ccagcacaac ggcgaccgac gacgcgagca gccacagcag caccgcgccc
     1801 cagaacacgc gggtgaccag ggccaggagg gcaggaacga cgttcccgac cacgccgaac
     1861 gtcgcgcgcg acacgaccat tgtcggctgc ccgctgcgct taccagcgag tgtcgacagg
     1921 ccgagcggaa tgaacgacag tgcgacgccg gcgagcacgg agacgatcga ctggcgcagg
     1981 ctcatcccca ccgcgaagac ggcggctccg agaccgaggc tcaggatgct cgagttcgcc
     2041 gcgaaccaca accagaagag ccgcgaggcc cggccgatcc tgtggtcgac gggcgtcggt
     2101 tccaggccgc tggtctcgac gctgaagacc cgctgacgca cgggttcgtt gtcgatgaga
     2161 acaacctcgt cctcgggaat gcgcaccgtg gagatcggac ctgacggcgg cgacacgatc
     2221 gcggcgccgc cgacaagccc ggcgaaggcg cgatcggtgt cgtcgacgtc gtcgtcgagc
     2281 tcctcctcgt aggcacgatc gattacagcg atcgagtcag tgtcgagagg aacgccctgg
     2341 cttgccgtga ccggggctgg ctcctcgaac agcgtctccg ccgcgaaggg acgcccctca
     2401 tcgacctccg gacgcaggtc gtcgtcggcg gggccggaga gcaggtcgtc gaagctgaat
     2461 gccgggggcg gagggggcgg cgcagccgag ggttcgtcct cgatgacctc tgcctcgatg
     2521 acctcctcgt actcggggat gggcacgatc gggatcggcg gatgccccgc cgcttgcgag
     2581 ggcagcagcg gcggcccgcc cagaatcggc ggttcgacga gaggcggcgg accggagacg
     2641 agtggaggtt ggggctcgaa cacgggctcg ggctccggca acggctccgg ttcgaccacg
     2701 agctccggct ccggtgcagg ctcaggctcg ggctcgggct cgggctcggg ctcgctgagc
     2761 gtcgaagccc agcccgcact cggcggcgga acatcccatg ccggaagcgg cggaggcgcg
     2821 gcgaccacgg gctccggctc gaccacaggc tccaacacga cgggctcgac gggcttgacg
     2881 ggaacggtgt cattgacccg gggcgcgtca tcggtctgcg gtgctggcca ggcagctacc
     2941 ggctgatcct cgacgacgaa gctctctgcc agcgaaaccg gcgcaacgtc ggccggcgga
     3001 acgtcaatcg gctgctcggt cgggccgacc gggatgccgc gagcgctcag ctgcgcttcg
     3061 agctgctcga tcgcgctcag cgtgccgcca gcggtttcga ggccagcctg ctcgagaagg
     3121 ctgagcagct cggggtcgcg cacgacgggt tccggcggtc cctccggaag aacgacggcc
     3181 tgagccggat ctggcgcggg gagtgagctc cacgccggta gctctgccac agggccgggc
     3241 tccatggtcg gcgcgggtgc agcctcaaca ggttcaggcg caggttccgg cacaagctcg
     3301 gagacagact cgggcacagg gtcagtcact accggaatcg acccggacga caagcgtccg
     3361 aggtcggccg cgagggcatt ggcgagctcg tcatcgtcgt gctcggcagg gggttcgggc
     3421 gaggcgggaa cgctccccgt gggcggggtg aacgtcgatc ggcgtggccc ggacgtggca
     3481 aatgtgggat cgaatgaggt ttcatcgaaa acctcgtcgt cgcttgccat gcagaaatac
     3541 tacggtgact ggcccggcga gcgtggttgc cgggccgtag cgagcacagt tactgcaccg
     3601 agaatgagga tcaatccggc ccagccgagc agttccaggc gctcaccgac caccagaaca
     3661 gcaaggatcg tcgcgacaac gggctccagc agcgtgatgg tcgtcgcagt actgctgcgc
     3721 agcgtgcgca tgcccacgcc gaacagcagg taggcgacga acatgggccc gagtgcgagg
     3781 taggcggcga ttcccaccgt cgtcgtcgac tgcagaaggg gcgctccgaa caccaggagc
     3841 acgggaagaa gcgcgatcgc cccgagtccg aacatgctcc ccatgaccgc acgcgagggc
     3901 tgtccggtgc cgatcgcacg actcgacgcg taggtgtaga gggcgtaggc gagccccgcg
     3961 gcaactccca ggccgatgcc cagcaggggc gaagtggctt gcggtgcgtc atcccgatga
     4021 ctgcccaggg ccagaagaat cacgcccacg atcgcgaccg cagtgcagag agcccatcgc
     4081 agggtcagcc tgcgtcgctc gacgatccac tccagaagcg cggcgaacac ggggccggtg
     4141 ccgagagaga cgacgttgcc gatcgcgacg ccggcgagac tcatcgacga gtagaacgcg
     4201 aggggatata cgaatacacc cacggcaccg atcagcagcc atcgacgact ggatgacgcc
     4261 cgcacggcgg ccagcgccgg gcgtgccgac acgaggaaga gaagcagccc accgatcgtc
     4321 atcgtgctcg cgccgaccgc gagcggactc accgagtccg ggagaaagct tgcggccgtt
     4381 cccgtggtcc cccacagcat cgacgcgaga atgagcgccc acacacccga cgaactcgct
     4441 gccggaatac ccctcacatc acgaggctag cggctctaat gtgagtgtca tgcgcgcacg
     4501 tatccccgcc ctcgttgccg ccctcgcggt ggtcgttcct cttgccgcgt gttccccagc
     4561 cccgcagccg agcgtcgcgc ccgccacggc ctccggctcc tggacggtgc t
//
LOCUS       DAOSOU010000176         4587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18879_length_4587_cov_6.319947, whole genome shotgun sequence.
ACCESSION   DAOSOU010000176 DAOSOU010000000
VERSION     DAOSOU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18879_length_4587_cov_6.319947"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLW15_09205"
     CDS             complement(<1..1097)
                     /locus_tag="PLW15_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT34608.1"
                     /translation="MASLAEILILRGVMPIESLDTMTSARADEEHLVLDLLERGVITE
                     AQLASARAAQAGLPFVELLDFPIDHSAVSKVPAAMCRRYEVLPIGYSGGYLILAMVDP
                     GNVFAVDDVRAATGQQVKCVVAERSDLRAAIDRYHRADGELSSLRTELEEVASADLVR
                     QDQNEPKEDDAPIVRFVNLLISQGIQDMASDIHIEPGEYDMGVRYRIDGVMHEMQRAP
                     KNIQNGVISRLKIMADIDIAERRRPQDGRMSVSHGGRKIDLRVATLPTVWGEKVVMRI
                     LDNSSSGMSMDDLNLLPRNLEVYKKSYSKPYGMILITGPTGSGKSTTLYTTLTAVARP
                     EINVITVEDPVEYRMKGINQVQVNPKAGLTFA"
     gene            complement(1182..1619)
                     /locus_tag="PLW15_09210"
     CDS             complement(1182..1619)
                     /locus_tag="PLW15_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34609.1"
                     /translation="MNRPAIHDLLGTILSTWYQTAAAWVPPGVLSEHTCDTCSTSLLA
                     DAVDVAAWPHDLMHDLATALNRAVEEISESWADDGCSDGLDEDGRMLCVFTLVRTTVM
                     EHREDLLDVLAECVEPTLADYLEAETIRGIASLAEWSNGNPGR"
     gene            1786..2445
                     /locus_tag="PLW15_09215"
     CDS             1786..2445
                     /locus_tag="PLW15_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT34610.1"
                     /translation="MAVDAGAGALRIAHVDDHETVQLGFAFLLADQPDITLVSSVFTV
                     DDLVPRLGEIDLVVLDIRLSDGSTIERNLALLQQHDARVLAFTAADNPAELRAASRAG
                     VLGIVRKSESRDVILEAIRSAARGSTVATTEWAAALDADPLLSSAGLSPKEREVLALY
                     AAGDKSVTVAHRAGLSAATVAEYVRRIRYKYAMAGRPAATKVDLYKRAVEDGILPSPE
                     P"
     gene            2442..3629
                     /locus_tag="PLW15_09220"
     CDS             2442..3629
                     /locus_tag="PLW15_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT34611.1"
                     /translation="MNPAVAPAGPQAAARVARTITLVAGVATLVFFGLSLGPIISSLP
                     YLDPWYDTGSAVALFGIPVVIAVVGQFVSLPTLRRMLGAYAIVFLVAVVAWVPALNAP
                     LPAGLAPWPLEVTALGTVPAAIAWRPAWVWVYLAVNSIVIAPVRWVSSGQHDWTVPLQ
                     YTFFTLTFAAIFTALAMVAMRNGYALDAATELARTTSASAAAAASRAQEQARLDALVH
                     DNVMATLFYASRGGLDESVRRQAQTTLTQLEQLRGGRDQATDDVSVEDFVARIRSVVL
                     DVAPDIAFVTTGHRQRPIPPDVAAAFAEATSEAVRNSLTHAVDRGDGVTRRASISLTD
                     VAVTVTVTDDGQGFEPRDVAPHRLGILVSIRGRLGAIAGGSARVDSGRGLGTAVTLTW
                     TSA"
     gene            3626..>4587
                     /locus_tag="PLW15_09225"
     CDS             3626..>4587
                     /locus_tag="PLW15_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34612.1"
                     /translation="MSATAETRQLEMIKLNGRGAHVMMWLYFATLTVLAIWSLNEVRT
                     AWPTLVAIALFAAVCVVLTLDPQEKVSLRTTLVAIAVWPIVAALVSWQLESGGGFSQW
                     YLGAGTAALFYISLRGRPVWAWIGFVLLSAVIVVWGATTAVGAGTALLLAAKQFPILV
                     VGTLFAIGLERTAASIQQLTAETSARAAIEAADVAATAERNNRLAELDAFATPLLILL
                     VSGAELTEEDRREFAVAEAELRDGLRARSISVPPIIEAARQARRRGVDVVLLDDSDPA
                     VVRPEDLELVITRISAALTDAQDGRLVARLLPPGREGVATLLVDG"
BASE COUNT          687 a         1547 c         1570 g          783 t
ORIGIN      
        1 gcgaaggtca aaccggcctt cgggttgacc tgcacctggt tgatgccctt catgcggtac
       61 tcgaccgggt cctcgacggt gatcacgttg atctcgggac gcgcaacggc ggtcagcgtc
      121 gtgtacagcg tggtcgactt accggaacca gtcgggcccg tgatgaggat catcccgtag
      181 ggcttcgagt acgacttctt gtagacctcg aggttgcgcg gaagcaggtt caggtcgtcc
      241 atgctcatgc cgctcgacga gttgtcgagg atacgcatga cgaccttctc accccacacc
      301 gtgggcaggg ttgccacgcg gaggtcgatc ttgcgaccgc cgtggctgac cgacatgcga
      361 ccgtcctgtg gacgacgccg ctcggcgatg tcgatatcgg ccatgatctt gaggcgcgag
      421 atcacgccgt tctgaatgtt cttgggggcg cgctgcatct cgtgcatgac gccgtcgatg
      481 cggtaccgca cgcccatgtc gtactcaccg ggctcgatgt ggatgtccga cgccatgtcc
      541 tggatgccct ggctgatgag caggttgacg aagcgcacga tcggcgcgtc atcctctttg
      601 ggctcgttct ggtcctgacg caccaggtcg gcggatgcga cctcctcgag ctccgtgcgc
      661 aggctggaga gttcgccatc ggcgcggtgg tagcgatcga tagcggccct gaggtcgctg
      721 cgctcggcaa ccacgcactt gacctgttga ccggtcgcag cgcgaacgtc atcgacggca
      781 aagacgttgc cggggtcgac catcgccagg atgaggtacc caccggagta gccgatggga
      841 agaacctcgt agcggcggca catcgccgcc ggcaccttcg agaccgcact gtggtcgatg
      901 gggaagtcga gcagctccac gaagggaagg cctgcctggg cggcgcgcgc cgaggcgagc
      961 tgggcctcgg tgatgacacc gcgctcgaga agatccagaa cgaggtgctc ctcgtcggcc
     1021 cgagcgctcg tcatggtgtc gagactctcg atgggcatga cgccccgcag aatcagtatc
     1081 tcagctagag acgccacgac ttcacccccc agtgatagcg actctctacg gtatccgcgg
     1141 cgcgtcggcg ccactagccc cgcaatagag gggtaggggg atcagcggcc ggggttgccg
     1201 ttcgaccact cggcgaggct cgcgatgcct cggatggtct cggcctcgag gtagtcggcg
     1261 agggtcggct ccacacactc ggccagaaca tcgagcagat cctcacggtg ctccatgacg
     1321 gtggtgcgca cgagggtgaa cacgcagagc atgcggccgt cctcgtcgag gccgtcggag
     1381 catccgtcgt cagcccatga ttcgctgatc tcctcgacgg cgcggttcag cgcggtcgcg
     1441 aggtcgtgca tgagatcgtg aggccatgcc gctacgtcga cggcgtcggc caggagagac
     1501 gtcgagcagg tgtcgcaggt gtgctcggac aggacccccg ggggcaccca tgcggcagcc
     1561 gtctggtacc acgtggacag gattgtgccc agaaggtcgt ggatggcagg tcggttcacg
     1621 agtgggtgct cccggtcggg tatctcaggg tccttctgaa tgctggcagg ttgcccctca
     1681 gggcgtcagt cgtccgatgc aatgacaccc gttcgggggt caacggcatg ctcaccggat
     1741 tcgtggcatc ctcatctgaa gacactcacg cgagaggggc ccggcatggc agtcgatgcg
     1801 ggtgctggcg cgctgcggat cgcgcatgtc gacgaccacg agacggtgca gctgggtttc
     1861 gctttccttc tcgctgacca gccggacatc accctcgtgt ccagcgtgtt cactgtcgac
     1921 gacctcgtgc cccgactcgg cgagatcgac ctggttgtgc tcgatatccg actctccgat
     1981 gggtcgacga tcgagcgaaa cctcgcgctg ctgcagcagc acgatgcacg agtgcttgcc
     2041 ttcactgccg ccgacaatcc tgccgagctg cgtgccgcgt cccgggccgg cgtgctgggc
     2101 atcgtgcgca agtcggagtc gcgcgacgtg atcctggagg cgatccgcag tgctgcgcgg
     2161 ggatcgacag tcgcgaccac tgagtgggcc gccgcactcg acgcggaccc ccttctgagc
     2221 tcggcgggac tcagccccaa ggagcgggag gtgctcgcgc tgtacgcagc gggtgacaag
     2281 tcggtgaccg tcgcgcaccg cgccggtctg tcggcggcca ccgtcgccga gtacgtcagg
     2341 agaatccgct acaagtacgc gatggcggga cggccggcag cgacgaaggt cgacctctac
     2401 aagcgtgccg tcgaggacgg catactgccg tcaccggaac cgtgaacccc gccgttgcac
     2461 ccgcgggacc ccaggccgcg gcccgggtcg cccgcaccat caccctcgtg gccggcgtcg
     2521 caacgctcgt cttcttcggc ctctcgctcg ggcccatcat ctcgtcgctg ccctacctcg
     2581 acccctggta cgacaccggc tcggccgtgg cgttgttcgg catccccgtc gtgatcgcgg
     2641 tggtcggaca attcgtgagt cttcccaccc tgcgacggat gctcggtgcc tacgccatcg
     2701 tcttcctcgt ggcggtcgtg gcgtgggtgc ccgcgctgaa tgccccactg cccgcgggcc
     2761 tcgccccgtg gcctctggag gtcaccgcgc tcgggactgt gccggccgcc atcgcctggc
     2821 gcccggcctg ggtgtgggtc tatctggccg tgaactcgat cgtgatcgcg cccgtgcgat
     2881 gggtgtcgtc aggacagcac gactggaccg tgccgctgca gtacaccttc ttcacgctga
     2941 ccttcgccgc gatcttcacc gcactcgcga tggtcgcgat gcgcaacggc tacgcgctcg
     3001 atgcggcgac cgagctcgcc cgcaccacgt ctgcgagcgc ggccgccgcc gcctcgcgcg
     3061 cccaggagca ggcccgcctg gacgccctcg tgcacgacaa cgtcatggcg accctgttct
     3121 acgcatcgcg aggtgggctc gacgagtcgg tcagacgaca agcccagacg acgctgaccc
     3181 agctcgagca gctgcggggc ggacgcgacc aagcgaccga cgacgtgtct gtcgaggact
     3241 tcgttgcgcg catccgctcg gtggtgctgg acgtggcgcc ggacatcgcg ttcgtgacga
     3301 ccgggcaccg ccagcgcccg atcccgccgg atgtggccgc cgcattcgcc gaggcgacgt
     3361 cggaggcggt gcgcaacagt ctcacgcacg ccgtcgatcg gggcgacggc gtcacgaggc
     3421 gcgcctccat ctccctcacc gatgtcgccg tcacggtgac cgtcaccgac gacggccagg
     3481 gcttcgagcc gcgggatgtc gccccccacc gcctcggcat tctggtgagc atccgcgggc
     3541 ggctgggcgc gattgccggt ggctccgccc gcgtcgactc cggccggggg ctcggcacgg
     3601 ccgtcacact cacctggacg agcgcatgag cgcgacggct gagacccggc aactcgagat
     3661 gatcaagctc aacgggcgcg gtgcgcacgt gatgatgtgg ctgtacttcg ccacgctcac
     3721 cgtgctcgcg atctggtcgc tgaacgaggt acgcaccgcg tggcccaccc tcgtggcgat
     3781 cgcgctcttc gccgccgtgt gcgtggtgct gaccctggac ccccaggaga aggtgtcgct
     3841 gcgcaccacg ctcgtcgcga tcgccgtctg gccgatcgtc gccgccctgg tgtcgtggca
     3901 gctggagtcc gggggcggat tctcgcagtg gtacctcggc gcgggtaccg ccgcgctgtt
     3961 ctacatcagc ctgcggggtc gacccgtctg ggcatggatc ggcttcgtgc tcctgtcggc
     4021 ggtgatcgtg gtctggggtg cgacgaccgc ggtcggtgcg gggaccgccc tgctgctcgc
     4081 ggccaagcag ttcccgatcc tggtggtcgg cacgctgttc gcgatcgggc tcgagcgcac
     4141 ggcagcgagc atccagcagc tgacggccga gacttcggcg cgcgcggcaa tcgaggcggc
     4201 ggatgtcgcg gcaaccgccg agcgcaacaa ccgccttgcg gagctcgacg ccttcgccac
     4261 cccgctgctc atccttctcg tcagcggggc ggagctcacc gaagaggatc gacgcgagtt
     4321 cgccgttgcc gaagccgagc tgcgggacgg gctgagagcc cgcagcatca gcgtgccgcc
     4381 gatcatcgaa gcggcccgtc aggctcgtcg ccgcggcgtc gacgtcgtgc tgctggacga
     4441 cagcgacccg gcggtcgtgc gtcccgagga cctggagttg gtgatcacgc gaatcagcgc
     4501 cgcgctcacc gacgcacagg atggccgact ggtggcccgc ctgctcccgc cggggcgcga
     4561 gggcgtcgcg acgctgctcg tggatgg
//
LOCUS       DAOSOU010000177         4571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_18968_length_4571_cov_6.826174, whole genome shotgun sequence.
ACCESSION   DAOSOU010000177 DAOSOU010000000
VERSION     DAOSOU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18968_length_4571_cov_6.826174"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1024)
                     /locus_tag="PLW15_09230"
     CDS             complement(131..1024)
                     /locus_tag="PLW15_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34613.1"
                     /translation="MTESLGEVAAITHRLAVLLAAGVAPVSAWSHVAVGATSAVPAAA
                     AASGPAGIPGAIVAAATGGDASARQAWRGLAAAWAVATDAGAPLAPSLTSYASSLRAL
                     ADAQRDVRVALAGPKSTARIVMVLPAIGVLFGLALGFNTLGVLVTTPPGWMCVAAGAA
                     LMLLARYWTRRLLASAQPADTTPGLDHDLAAIAVSGGGALDRAVAAVHEAGATYGITV
                     GDLDATLDLSRRAGVPAGDLLRGEAEERRRAARADAQERAAALSVRLMLPLGLCVLPA
                     FMVLGVLPLLIAVVSSTVGTF"
     gene            complement(1021..1956)
                     /locus_tag="PLW15_09235"
     CDS             complement(1021..1956)
                     /locus_tag="PLW15_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HOT34614.1"
                     /translation="MSAQRVDRGVDYGPLEGVMSEPHVTDVFVNGPRQLWVDRGDGLV
                     RHPFTLDEPELRELATRLVWAGGRHIDEASPTVDARLPGGVRVHAVLPPISPAGTLLS
                     IRVPSPHPLTLADLEARGFFGEVALERVRALVAARTNLLITGASGSGKTTLLSALLGS
                     APDGERIVAIEDVAELRPGHPHFVCLEARQANLEGAGGLGLARLLRESLRMRPDRLVL
                     GECRGEEIRELLSALNTGHDGGAGTLHANSLADVPARLEALGALAGLDPWAIARQAVS
                     AIGAVLHIERAGGMRWLAGIARFALDGAGRLTVVP"
     assembly_gap    2177..2276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2673..>4571
                     /gene="acs"
                     /locus_tag="PLW15_09240"
     CDS             2673..>4571
                     /gene="acs"
                     /locus_tag="PLW15_09240"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOT34615.1"
                     /translation="MSTNSIDSLLHEDRHFPPSADFAANSVASASLYTDAKADRLAFW
                     ADRAREYVHWHKPFTEVLDWSNAPFAKWFADGELNVAYNCLDRHVEAGIGDRVAIHWE
                     GENGDAVSLTYAQLTAEVKRAANAIASLGIGKGDRVAIYMPVVPEAVIAMLAVARLGA
                     VHSVVFGGFSAESLRSRIDDAEAKLVITADGGWRKGKVFPLKPAVDAALANGTSTVSS
                     VLVVKRGDNDVEWVDGRDLWWHDAVEAAEPEHVAQAFESENPLFILYTSGTTGKPKGI
                     LHTSGGYLTQVAFTHKNVFDLHPETDVFWCTADVGWITGHSYVVYGPLANGATQLLYE
                     GTPDTPHPGRWWELIEKYKVSIFYTAPTAIRSFMKLGRQIPQGFDLSSLRLLGSVGEP
                     INPEAWIWYREVIGANVAPIVDTWWQTETGAIMISALPGLTTLKPGAAQTPIPGIRID
                     ILGEDGTRVDPPQGGLLVVSEPWPSMLRGIWGDPDRYVDTYWSKFSKPEEGVYKYFAG
                     DGAHVDTDGDIVLLGRVDDVMNVSGHRLSTQEIESALVSHHMVAEAAVVGAADEQTGQ
                     AVVAFVILKANQTDAHTADEASTLLRSHVASAIGAIARPRQIFIVAELPKTRSGKIMR
                     RLLQDVAEG"
BASE COUNT          730 a         1585 c         1556 g          600 t
ORIGIN      
        1 gcggcgagcg tggcgatcgc gtactcggcg gtggcggcgc cggtgtcatc ccgaatccag
       61 ttcttcatgg tgtcagcctg cggcggcggc cacgggggtg ttgtcggcct cgaggcatcc
      121 ggacacagga tcagaacgtg cccactgttg acgagacgac cgcgatcagt agcggcagca
      181 cgccgagcac catgaacgcc ggaagcacgc acagcccgag cggcagcatg agccgcaccg
      241 acagggcggc cgcgcgctcc tgcgcgtcgg ctcgggcggc ccggcgccgt tcctccgcct
      301 cgccgcgcag gaggtcaccc gccggcacgc ccgcgcgacg cgacaggtcg agagtcgcat
      361 ccaggtcccc gacggtgatg ccgtaggtcg cgccggcctc gtgcaccgca gccaccgcgc
      421 gatcgagggc gcctcccccg ctgacggcga tggccgcgag gtcgtggtca agacccgggg
      481 tggtgtcggc gggctgcgcc gacgcgagta gtcggcgcgt ccagtaccgc gccagcagca
      541 tcagtgcggc acccgcggcc acacacatcc atccgggcgg cgtcgtgacc agcacaccga
      601 gggtgttgaa gccgagcgct agcccgaaca gcacaccgat ggcgggaagc accatcacga
      661 tgcgcgctgt cgacttgggg cccgccagcg ccacccgcac gtcgcgctgg gcatcggcga
      721 gcgcccggag cgagctcgca taggaggtga ggctgggcgc gagcggagcg cccgcatcgg
      781 tggcgaccgc ccaagcggcg gcgagaccac gccacgcctg gcgtgcggac gcatcaccgc
      841 ccgtcgcggc ggcgacgatc gcccccggga tgcccgccgg cccggatgcc gcggccgccg
      901 ccggaacggc cgacgtcgcc cctaccgcaa catgcgacca cgccgacacg ggcgcgacac
      961 cggcggcgag cagcaccgcc agacgatggg tgatggcggc gacctcgccg agcgactccg
     1021 tcacggcacc acggtgaggc ggcccgcgcc atcgagcgcg aaccgcgcga tgcccgcgag
     1081 ccatcgcatc ccacccgcgc gctcgatgtg gagcacggcg ccaatggcgc tcacggcctg
     1141 acgcgcgatc gcccacgggt cgagtccggc gagcgcgccg agcgcctcga gccgcgccgg
     1201 tacgtccgcg agcgagttgg cgtgcagggt tcccgcgccg ccgtcgtgcc cggtgttgag
     1261 cgccgagagc agctcgcgga tctcctcgcc ccgacactcc cccagcacga gccgatcggg
     1321 gcgcatccga agcgactccc gcagcagtcg cgccaggcca aggccacccg cgccctcgag
     1381 gttggcctgc ctggcctcca gacagacgaa gtgcggatgc cccggccgca actccgcgac
     1441 atcctcgatg gccacgatcc gctcgccgtc gggagccgag cccagcaggg ccgacagcag
     1501 cgtcgtcttg ccgctgccgc tggcacccgt gatcagcaga ttcgtgcggg ctgcgacgag
     1561 agcacgcact cgctcgagcg ctacttctcc gaagaatccc cgagcctcga ggtcggcgag
     1621 cgtgagcggg tgcggcgagg gcacgcggat cgacaggagc gttcccgccg gcgagatcgg
     1681 gggcagcacg gcgtgcacgc gaacgccgcc ggggagccgg gcatccaccg tcggcgaggc
     1741 ctcgtcgatg tgcctgccgc ccgcccagac cagacgggtt gcgagctcgc ggagctcggg
     1801 ctcgtcgagc gtgaacggat gccgcaccag cccgtcaccc cgatcgaccc acaactggcg
     1861 cggcccgttg acgaacacat cggtcacgtg gggctcggac atcacaccct cgagcggccc
     1921 gtagtccacc ccgcggtcga cccgctgcgc ggagatggcg ggtggcgcgc cgggcggccg
     1981 cgcaacaaag gggaccaccg cgctatcgct caccccggca cgctacgcgc ggtcgaagca
     2041 ccgagtcgcg gcgcggctgc gggaggagta acggtcgccg gggccggagc ctgtggcgga
     2101 gaggaatcgg cacggcggga gtgacctgcg ggaggcggcc tggtttcgat acgcgctgcg
     2161 cgctactcaa ccagcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgac
     2281 ctgcgggagg cggcctggtt tcgatacgcg ctgcgcgcta ctcaaccagc gggagtgacc
     2341 tgtgggaggg ggggctggtt tcgatacgcg ctgcgcgcta ctcaaccagc ggactcacgc
     2401 aacccaacca tcagcgacgc gtcggcggcg gggacgccga cgccttaaat ggagaggggc
     2461 ggcgcccatt ggggggaacg ggcgccgcca cagcagtgcc ggattggggg gaatcactgg
     2521 caccacttgc cgcacttgcg tgcgaccgcc ccagtgtatc gcagtgcgtc tcaaactcaa
     2581 tgggaagttg ccgcgcggca ctaggatcgg acagttacct gttcaacagt tcgcggcatc
     2641 ctggcagccc cgcgagatcg acgaggatct gcatgtctac caattcgatt gacagcctgc
     2701 tccatgagga ccgccacttt ccaccgagcg ccgatttcgc ggccaactcg gtggccagcg
     2761 cgagcctcta cacggatgcc aaggccgacc gcctcgcctt ctgggccgac cgcgcccggg
     2821 agtacgtgca ctggcacaag cccttcaccg aggtgctcga ctggagcaac gcacccttcg
     2881 cgaagtggtt cgccgacggc gagctcaacg tcgcctacaa ctgcctcgac cggcacgtgg
     2941 aggccggcat cggcgaccgg gtcgccatcc actgggaggg cgagaacggc gacgccgtca
     3001 gcctcaccta cgcgcagctg accgccgagg tcaagcgcgc cgccaatgcg atcgcgtcgc
     3061 tcggcatcgg caagggcgac cgggtcgcga tctacatgcc ggttgtgccg gaggcggtca
     3121 tcgccatgct cgccgtcgcc cggctcggcg cggtgcactc ggtggtgttc ggcggcttca
     3181 gcgccgagag cctgcgcagc cgcatcgacg acgccgaagc caagctggtc atcaccgccg
     3241 acggcggctg gcgcaagggc aaggtcttcc ccctgaaacc cgccgtggat gccgcactcg
     3301 ccaacggtac gagcaccgtg tcgagcgtgc tggtcgtcaa gcgtggcgac aacgacgtcg
     3361 agtgggtgga cggccgggac ctgtggtggc acgacgccgt tgaggcggcc gagcctgagc
     3421 acgtggcgca ggcgttcgag tccgagaacc cgctgttcat cctctacacc tcgggcacga
     3481 cgggcaagcc gaagggcatc ctgcacacct ccggcggcta cctcacgcag gtggcgttca
     3541 cccacaagaa cgtcttcgac ctgcacccgg agaccgacgt cttctggtgc accgccgacg
     3601 tcggctggat caccgggcac agctacgtgg tctacggccc gttggcgaat ggcgccacgc
     3661 agctgctcta tgagggaacg cctgacacgc cgcatccggg ccgctggtgg gagctcatcg
     3721 agaagtacaa ggtctcgatc ttctacacgg cgccgacggc gatccgctcg ttcatgaagc
     3781 tggggcggca gattccgcag ggcttcgacc tgtcaagcct gcggttgctc ggctcggtcg
     3841 gcgagccgat caacccggag gcctggatct ggtaccgcga ggtcatcggc gccaatgtcg
     3901 caccgatcgt cgacacctgg tggcagacgg agaccggagc catcatgatc tccgcgctgc
     3961 cagggctcac cacgctcaag ccgggtgctg cgcagacgcc gattccgggc atccgcatcg
     4021 acatcctcgg tgaggatggc acgcgcgtcg atccgccgca gggcggcctg ctcgtggtca
     4081 gcgagccgtg gccgtcgatg ctgcgcggca tctggggcga ccccgatcgc tacgtcgaca
     4141 cctactggtc gaagttctcc aagcccgagg agggcgtcta caagtacttc gccggcgacg
     4201 gcgcgcacgt cgacaccgac ggcgacatcg tgctgctcgg ccgggtcgac gacgtcatga
     4261 acgtgtcggg gcatcgactg tcgacgcagg agatcgagtc ggcgctcgtg tcgcaccaca
     4321 tggtcgccga ggccgcggtc gtcggagccg ccgacgagca gaccggtcag gccgtggttg
     4381 ccttcgtgat tctgaaggcc aaccagacgg atgcccacac cgccgacgag gcatcgacgc
     4441 tgctgcgctc ccacgtcgca tcggcgatcg gcgcgatcgc gaggccccgc cagatcttca
     4501 tcgtggccga gttgccgaag acccgctcgg gcaagatcat gcgtcgcctg cttcaggatg
     4561 tcgctgaggg c
//
LOCUS       DAOSOU010000178         4547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_19114_length_4547_cov_5.722841, whole genome shotgun sequence.
ACCESSION   DAOSOU010000178 DAOSOU010000000
VERSION     DAOSOU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19114_length_4547_cov_5.722841"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLW15_09245"
     CDS             <1..761
                     /locus_tag="PLW15_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT34616.1"
                     /translation="AAVESAAAAVRETDPAIVGPWTDDIELLLEERRRRLGAPEPVEV
                     PTRVPASRFKDYVSEPDAVASGLRRPMPEQPYRATQLGTLFHSWVENRFGPGGDAEQL
                     AAATVGADELDAAPGETDAGDTLTDADLDRLKATFEASPWAARRPVEVEREIHLPFDG
                     RIIICKIDAVYEVDGRFEVVDWKTGKAPKDAADLEQKQLQLALYRLAYSKWRGIDPDL
                     IDAVFYFVADNRVIRPDHIYDESELLERWRAALA"
     gene            788..1576
                     /locus_tag="PLW15_09250"
     CDS             788..1576
                     /locus_tag="PLW15_09250"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HOT34617.1"
                     /translation="MNFFFYDGPIWIALPTFVLLFLAFSWAILLAVRPWVKRVSANHE
                     EWDRVLGYAMQSYGIFYGILLALIAVSVYENFQRVSGVVLDEASSLGTLYRAVSSYPP
                     PVGPDLQEQLRLYTQNVISVDWPLQGRDIIPAEGNAQVDSIQSILFAYEPSTSGGQAL
                     HNQTVGLFFDFVEARRARLDETKLALPPLLWVLVAVGAALNALMLALVEARNLRVHLI
                     MSGLIAVFVALLIFVTAAMDHPYSGFVSVPPEPFQNLLEQAMKP"
     gene            complement(1604..2452)
                     /locus_tag="PLW15_09255"
     CDS             complement(1604..2452)
                     /locus_tag="PLW15_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOT34618.1"
                     /translation="MFDPVLLRSFVAVAETLSFTQSAHQLSLSQPTVSQHIRKLELAA
                     GRNLVTRDTRSVALTDNGEAMLGFARTILAAQDQAQSYFTGSAMRGRLRFGSADDLAL
                     TQLPGILRDFRQLYPQINLELTVSQSPTLLRRLAAGALDLVFIKQDPGSTEGRLVRRD
                     RMVWLGHRSMTLDRDAPVPLIAYQAPSLSRMYAIRSLESVGRMWRITCNVKEVNGVLA
                     AVRAGIGIAVFPQSLIPPDLAQVSANYELPELGDVDFSLLDNPSAPRGPIDALTAAIL
                     SRPVHQ"
     gene            2540..3847
                     /locus_tag="PLW15_09260"
     CDS             2540..3847
                     /locus_tag="PLW15_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34619.1"
                     /translation="MTDPATMPIAVAMQRPAWRDTFSALRLHNYRLYVVAQLISNTAG
                     WMQRIAVDWLVLELTGSVALVGLTIALQFLPTLVLGAHAGVIADRFSKRAVMLVCQGA
                     GGLLNLVLAILAITGNAQLLTVYALVLAVGIAQVFDGPARSVFITEMVGARHLRNAIS
                     VNASIFHLGALVGPALSGLLIVAVGSGWAIGLNVVAAAIGIVLLLAIRSADLHPAPRA
                     PNTKGQVREAVRYIMGKPSLFWTLVIVGVVAIFGMPMPALLAGVADSVYGTGATGYGL
                     YNSLAAVGALTGALASARRSSLRLRTIIFSALLYGLAMTLLGLAPFYPVFLALLVGVG
                     LSRLMLMTAGETMMQFSSNLAIRGRVMAFWAMVIVGGQAIGGPLMGFIAEQIGPKNAL
                     VVAGAVPAVAAVAIALLLARSGRLKMRVTPRRGRWVAIVPRSS"
     gene            complement(3868..>4547)
                     /locus_tag="PLW15_09265"
     CDS             complement(3868..>4547)
                     /locus_tag="PLW15_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT34620.1"
                     /translation="TPLDSYTAGPDSLAASVGRAIAAIHGLPTSFVADAGLPVMTAGE
                     CLRSCVTVMDRAAATGLVPAALLGRWERAAEDAKLWQFQPTVINGSLAADSFLQQDGS
                     VTGVLGWQDLRVGDPARDMQWLLGSHSDAIIDSAFDAYSRLRGASDRQLRQRATLYAE
                     LEVAKWLLHGTETRSTEIVDDAVEMLTALADNLRNDVMNPIGAQTMPTMAVDEVEAFL
                     DRNERAV"
BASE COUNT          674 a         1567 c         1511 g          795 t
ORIGIN      
        1 gtgcagcggt cgagagcgcc gccgcggccg tgcgggagac cgatccagcc atcgtcggac
       61 cgtggactga cgacatcgag ctgcttctcg aggagcgtcg ccgtcggctc ggtgccccgg
      121 aacccgtcga ggttcccacc cgcgtgccgg cttcccgatt caaggactac gtgagcgagc
      181 cggacgccgt ggcatcgggc ctgcgccgac ccatgccgga gcagccctat cgggcaaccc
      241 aactcggcac cctgttccac tcctgggtgg agaaccggtt cggtcccggc ggagacgccg
      301 agcagcttgc cgccgcgacg gtcggtgccg acgagctgga tgccgcaccc ggcgagaccg
      361 acgccggaga caccctcacc gacgccgacc tcgaccgcct caaggcaacc ttcgaggcca
      421 gcccctgggc ggcgcggcgg ccggtcgagg tggagcggga gatccacctg cccttcgacg
      481 gccgcatcat catctgcaag atcgacgccg tctacgaggt cgacggacgc ttcgaggtcg
      541 tcgactggaa gaccggcaag gcgccgaagg atgccgcgga tctcgagcag aagcagctcc
      601 agctcgccct ctaccgtctc gcctattcga agtggcgcgg catcgatccc gacctcatcg
      661 acgcggtgtt ctacttcgtc gccgacaatc gcgtgatccg gcccgatcac atctacgatg
      721 agtccgaatt gctcgaacga tggcgagccg cgctggcgta gcgcggcaca cgcagacggg
      781 aggcgcggtg aacttcttct tctacgacgg tccgatctgg atcgcgctgc ccacgttcgt
      841 gctcctcttc ctcgcgttct cgtgggcgat cctgctcgcc gtgcgtccgt gggtgaagcg
      901 ggtgtcggcc aaccacgagg agtgggaccg ggtactcggg tatgcgatgc agtcctacgg
      961 gatcttctac ggcatcctgt tggctctcat cgcggtgtcg gtctacgaga acttccagcg
     1021 cgtcagcggt gtcgtgctcg acgaggcatc ctcgctcgga accctgtacc gggcggtctc
     1081 gtcgtacccg ccgcccgtcg gccccgatct gcaggagcaa ctgcggctgt acacgcagaa
     1141 cgtcatctcc gtggactggc ctcttcaggg ccgcgacatc attccggccg agggcaatgc
     1201 ccaagtcgat tccatccagt cgatcctctt cgcctacgag ccctcgacca gcgggggaca
     1261 ggccctgcac aaccagaccg tgggtctgtt cttcgatttc gtcgaagccc gccgcgctcg
     1321 gctggacgag acgaagctcg ccctgccgcc gctgctgtgg gtgctcgtcg ccgtcggggc
     1381 cgccctcaac gccctgatgc tcgcgctcgt cgaggcgcgc aacctgcgcg tgcacctgat
     1441 catgtccggc ctcatcgccg tgttcgtcgc gctgctgatc ttcgtgacgg cggccatgga
     1501 tcacccgtac tccggcttcg tcagtgtgcc gcccgagcca ttccagaacc tgctcgagca
     1561 ggcgatgaag ccctgaccgg tggtgcccgc cctgacgcag ggcctactgg tgcacgggcc
     1621 tgctcaagat ggcggcggtc agcgcatcga tcggtccgcg gggcgcgctc gggttgtcga
     1681 gcagcgagaa gtcgacgtcg ccgagttcgg gaagttcgta gttggcggag acctgcgcca
     1741 ggtccggggg gatcagcgac tgggggaaca ccgcgatccc gatgccggcc cgtaccgccg
     1801 cgagcactcc gttgacctcc ttgacgttgc acgtgatgcg ccacatgcgg ccgacggatt
     1861 ccagggagcg aatcgcgtac atgcgactga ggctcggcgc ctggtaggcg atcaggggca
     1921 ccggggcatc ccggtcgagg gtcatgctgc ggtgacccag ccacaccatg cgatcgcgcc
     1981 gcaccagtcg accctcggtg ctgcccggat cctgcttgat gaatacgaga tcgagggctc
     2041 cggcagcgag ccgacgcagc agtgtggggc tctggctgac cgtcaactcc agattgatct
     2101 gcgggtagag ctgccggaag tcccgcagga tgcccggcaa ctgcgtgagg gcgaggtcgt
     2161 cggccgaccc gaagcgcagg cgcccccgca ttgctgatcc ggtgaagtag ctctgggcct
     2221 gatcctgcgc ggcgagaatc gttcgggcga atcccagcat cgcctcgccg ttgtcggtga
     2281 gtgccaccga tcgagtgtcg cgggtcacca gattgcgccc ggcggcgagc tccagcttgc
     2341 ggatgtgctg gctcaccgtc ggttggctga ggctcagctg atgggccgac tgggtgaaac
     2401 tcagcgtctc ggcgacggcg acgaagctgc gcagcagcac cggatcgaac acggccagcc
     2461 acctctcatt acgtttcgct atgggactta ttgagccaat tatcccatcg tatgaatgac
     2521 ccgagactat cgtcgtaggg tgactgatcc cgcgacgatg ccgatcgccg tcgcgatgca
     2581 gcggccagca tggcgcgaca ccttcagcgc gctgcggttg cacaactacc gcctgtacgt
     2641 cgtcgcccaa ctcatctcga acacagccgg ctggatgcag cgcatcgccg tcgactggct
     2701 ggtgctcgag ctcaccggca gcgtggcgct ggtcggtctg acgatcgccc tgcagttcct
     2761 gccgacgctg gtgctcggcg cgcacgccgg agtcatcgcc gaccggttct ccaagcgtgc
     2821 cgtcatgctg gtctgccagg gcgccggcgg gctgctcaac ctcgtgctcg cgatcctggc
     2881 gatcacgggc aatgcccaac tgctcaccgt gtacgcgctg gtgctcgcgg tcggcatcgc
     2941 ccaagtcttc gacggtccgg cccgctccgt cttcatcacc gagatggtcg gggctcgtca
     3001 cctgcgcaat gccatcagtg tcaacgcgtc gatcttccac ctcggcgcac tcgtcggccc
     3061 cgccctgagt ggcctgctga tcgtggccgt cggatcgggg tgggcgatcg gactcaacgt
     3121 ggtcgccgcg gcgatcggca tcgtgctgct gctcgcgatc cgctcggcag acctgcatcc
     3181 cgcgccccgg gcgccgaata cgaagggaca ggtgagggag gcggtgcgct acatcatggg
     3241 caagcccagc ctgttctgga cgctcgtgat cgtcggtgtc gtcgccatct tcgggatgcc
     3301 catgcccgcc ctcctcgcag gcgtcgccga ctccgtgtac ggcaccggcg ccaccgggta
     3361 cgggctgtac aactcgcttg ccgccgtcgg agcgctgacc ggggcactcg catccgcccg
     3421 ccggtcgagt cttcgactgc gcacgatcat cttcagcgcg ctgctctacg ggctcgccat
     3481 gacgctgctc ggcctagcgc cgttctaccc cgtctttctc gcgctcctcg tgggcgtcgg
     3541 cctgagccgc ctgatgctca tgaccgcggg agagaccatg atgcagttct cctcgaacct
     3601 ggcgatccgc ggtcgcgtga tggcgttctg ggcgatggtg atcgtcggcg gtcaggcgat
     3661 cggcggcccg ctgatggggt tcatcgccga gcagatcggc ccgaagaacg ccctcgtcgt
     3721 cgcgggggcc gtgcccgccg tcgccgccgt ggcgatcgct ttgctcctgg cccgatcggg
     3781 tcgactgaag atgcgcgtca cccctcgccg cggtcgctgg gtcgcgatcg ttccgcgttc
     3841 gagctagacg gggccctccg gctcttccta gacggcgcgc tcgttgcggt cgaggaacgc
     3901 ctcgacctcg tcgacggcca tggtcggcat ggtctgcgcg ccgatcgggt tcatgacgtc
     3961 gttgcgcaga ttgtcggcga gagcggtgag catctcgacc gcgtcatcca cgatctcggt
     4021 gctgcgcgtc tcggtgccgt gcagcagcca cttggccacc tcgagctccg cgtacagggt
     4081 ggcgcgctgg cgaagctggc ggtcgcttgc accccgcagc cgggagtacg cgtcaaaggc
     4141 gctgtcgatg atggcgtcgc tgtgtgaccc gagcagccac tgcatgtccc tggccgggtc
     4201 gccgacccgc agatcctgcc agccgagcac gccggtcaca ctgccgtcct gctgcaggaa
     4261 cgagtcggcg gccagtgagc cattgatcac cgtcggctgg aactgccaga gcttggcatc
     4321 ctcggccgca cgctcccacc ggcccagcag ggcagcgggc accagcccgg tcgcggcggc
     4381 gcggtccatc acggtcacgc aggaacgcag gcactcgccg gcggtcatga ccggcagtcc
     4441 ggcgtcggcc acgaaactcg tcggcaggcc gtggatcgcc gcgatcgccc gaccgacgga
     4501 tgccgcgagc gaatccggtc cggcggtgta cgagtcgagc ggggtct
//
LOCUS       DAOSOU010000179         4500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_19363_length_4500_cov_4.121935, whole genome shotgun sequence.
ACCESSION   DAOSOU010000179 DAOSOU010000000
VERSION     DAOSOU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19363_length_4500_cov_4.121935"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PLW15_09270"
     CDS             complement(<1..1198)
                     /locus_tag="PLW15_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT34621.1"
                     /translation="MPQHENHDHPVVEPVETPVVEPVETHDDHHHAPVSTSSTTAHDH
                     DHHAHHHAPVSTSSTTGHDHHDMHAGHGGGDHSHHDPAIFRRKFWLSLILTIPTLVFS
                     TGLQDILGLAGPRFPGSQFIPAVFGIAIFLYGGLVFLRGAVTELKSRQPGMMTLISLA
                     VVVALGYSVAVTLGLPGMDFWWELSTLITIMLLGHWIEMSAVMGAQDALGELAKLLPD
                     EADLVHGDHVMTVPVSQLKLGDVVLVRPGAAIPADGEVVDGESHVNEALLTGESSSVS
                     KTTGDAVIGGSVNGSGALRVRVTKLGDDTAVAGIMKLVADAQASKSGAQLLADRAAAL
                     LFYVALGSAVITLVAWLIIMPGDPAFVLERVVSVLVIACPHALGLAIPLVSQISTALG
                     ARKGILV"
     gene            complement(1202..1483)
                     /locus_tag="PLW15_09275"
     CDS             complement(1202..1483)
                     /locus_tag="PLW15_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOT34622.1"
                     /translation="MIADIKKRALHRSRILEGQMRGLEKMIESEDYCMDIITQSLAIQ
                     KSLRSLNKLLVENHLRTHVSDMFAAGGVERQHAVDELLKVYELNNNRSE"
     gene            1545..2180
                     /locus_tag="PLW15_09280"
     CDS             1545..2180
                     /locus_tag="PLW15_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT34623.1"
                     /translation="MKLTKYEHACMVIEKADVSLVIDPGGYTMPLTDLLNVVAVVITH
                     EHGDHWTAEQLTRIKERNPKVRFFGPSGVAAAAPDFEIEIVRAGDTVTVEPFTLRFFG
                     EKHAVIHESIPLVDNVGVLVNDELYYAGDSYTVPGVSVPVLAAPVGAPWLKIGEAMDY
                     VLAVKPRQAFPVHEAVLSQIGRNLGNDRLRNATEQGGGEFVVLEAGESLEL"
     gene            2185..2763
                     /locus_tag="PLW15_09285"
     CDS             2185..2763
                     /locus_tag="PLW15_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT34624.1"
                     /translation="MTITVRPATEVPWDDVRTVFGTRGDPSRCWCQWFKVTNAEWEST
                     EPAQFEQMLCDQVSAATVSPGLIAYADGEPAGWAAVEARPGYRRLTTMKVAAASPEPQ
                     GDPAVWAVTCFVVRVGYRKRGVSAALLAGAMEFARSHGARLLEAYPVDVAEKKTSSAD
                     LFHGALSTFLSAGFEVVARPSDGRAVVQLALQ"
     gene            complement(2725..3687)
                     /locus_tag="PLW15_09290"
     CDS             complement(2725..3687)
                     /locus_tag="PLW15_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34625.1"
                     /translation="MTTAVQNRPTSTVAGIRWHRPLLVLAAAMGVLVVVCLVGLVVDP
                     RESLGVNVWEKPLKFAISTAVYAVTWSWLIGQLQRFQRVAWWAGTISAILLLIELVII
                     TGAAAAGITSHFNVSTPFNTVLWSIMAASIGTLWVATFVVSIILFRNSLGDRARTLAI
                     RAGALIALAGMALAFLMTGPTAAQLDDWQGIAGAHTVGLADGGPGLPLLGWSTVAGDL
                     RIPHFIGMHALQAIPLALILIELAARRIAVLRDVDVRYRLVAIVSAAYVAVLAVLTWQ
                     ALRGQSIVAPDAATATATLVIAALALAAAAVVLLQRQLHHRTAV"
     gene            complement(3684..4343)
                     /locus_tag="PLW15_09295"
     CDS             complement(3684..4343)
                     /locus_tag="PLW15_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT34626.1"
                     /translation="MKMSQLCEASGVSVPSIKFYLREGLLPAGERTSATQAEYGPEHV
                     ERLRLIRALIDIGGLSVATAQRVLEAIDSPDLPLSYVFGVAQYAISDTSLYADVPEGS
                     AGTTQVNDLIESTGWLVSDENPGRRGAARVLDTYADLGQKHIGEHLPAYAEAAELIAR
                     ADLAAVAHHADVADMAETVVVGTVLGDALNASLRRMAQEHVSYQVFPAPRGAEPSGSE
                     R"
BASE COUNT          732 a         1439 c         1624 g          705 t
ORIGIN      
        1 tcacgaggat gcccttgcga gctcccagcg ccgtcgagat ctgcgatacc agcgggatcg
       61 ccaggccgag cgcgtgcggg cacgcgatga ccagcacgct gacgacgcgc tcgagaacga
      121 acgccgggtc gcccggcatg atgatgagcc acgcgacgag agtgatgacc gccgacccga
      181 gggcgacgta gaacaacagc gcggccgccc ggtcggcgag gagctgcgcg ccggacttgc
      241 tggcctgggc gtcggcgacg agcttcatga tgcccgccac ggccgtgtca tcgccgagct
      301 tcgtcacgcg cacacgcagg gcgcccgaac cgttcaccga accgccgatc acggcgtcgc
      361 cggtcgtctt cgatacggag gacgactcgc cggtgaggag ggcctcgttg acgtgcgact
      421 cgccgtctac gacctcccca tcggcgggga tcgcggcgcc ggggcgcacc agcaccacgt
      481 cgcccagctt cagttgggac accgggacag tcatcacgtg gtcgccgtgc accaggtcgg
      541 cctcatcggg gagcagcttc gcgagctcgc cgagggcatc ctgcgcgccc atgacggcgg
      601 acatctcgat ccagtgtccg agcagcatga tcgtgatgag ggtggagagc tcccaccaga
      661 agtccatgcc gggcaggccg agcgtgacgg cgacgctgta gccgagcgcg acgacgaccg
      721 ccagagagat gagggtcatc atgcccggtt ggcgcgactt cagttcggtt accgctccgc
      781 ggaggaatac caggccgccg tagaggaaga tcgcgatgcc gaatacggcc gggatgaact
      841 ggctgccggg gaagcgcggg ccggcgaggc cgaggatgtc ctgcagtcca gtcgagaaga
      901 ccagcgtggg aatcgtcaga atcaggctca gccagaactt gcggcggaag atcgcggggt
      961 cgtggtggga gtggtcgccg ccaccgtggc cggcgtgcat gtcgtggtgg tcgtggccgg
     1021 tggtcgagct cgtcgagacc ggggcgtggt ggtgggcgtg gtggtcgtgg tcgtgagcgg
     1081 tggtcgagct cgtcgagacc ggggcgtggt ggtggtcgtc gtgggtctcg acaggctcga
     1141 ccaccggggt ctcgactggc tcgaccaccg ggtggtcgtg gttctcgtgc tgtggcatcc
     1201 gttactccga gcggttgttg ttgagttcgt agaccttgag cagctcatcg acggcgtgct
     1261 gccgctcaac tcccccagcg gcgaacatgt cgctcacgtg ggtgcgcagg tggttctcga
     1321 cgagcagctt gttgaggctg cgcagcgact tctggatggc cagggactgc gtgatgatgt
     1381 ccatgcagta gtcctccgac tcgatcatct tctcgagtcc gcgcatctgc ccctcgagga
     1441 tgcgcgaccg gtgcagggcg cgcttcttga tgtcagcgat catgagtccg atgatacccc
     1501 cggggggtat caagcgcaaa cggtcgcccc cttaggctcg acgcatgaag ctgacgaagt
     1561 acgagcacgc ctgcatggtc atcgagaaag ccgacgtgtc gctcgtcatc gatccgggcg
     1621 gatacacgat gcccctcacc gacctgctga acgtcgtcgc cgtcgtgatc acccacgagc
     1681 acggcgacca ctggaccgcc gagcagctga cccgcatcaa ggagcgcaac ccgaaggtgc
     1741 gcttcttcgg gccgagcggc gtggccgccg ccgcaccgga cttcgagatc gagatcgtca
     1801 gggcaggcga caccgtgacc gtggagccgt tcaccctgcg gttcttcggc gagaagcacg
     1861 cggtgatcca cgagtcgatc cctctagtcg acaacgtggg ggtgctcgtc aacgacgagc
     1921 tgtactacgc gggcgattcc tacaccgtgc cgggcgtctc agtgcccgtg ctggccgctc
     1981 cggtgggtgc cccgtggctc aagatcggcg aggcgatgga ctacgtgctc gccgtgaagc
     2041 cccgacaggc gtttcccgtg cacgaggccg tgctctccca gatcggccgc aacctcggca
     2101 acgaccgcct caggaacgcc accgagcagg gcggcggcga gttcgtcgtg ctcgaggctg
     2161 gcgagtccct ggagctgtga cccaatgaca atcactgtcc gccccgccac cgaggtgcca
     2221 tgggacgatg tgcgcacggt cttcggcacc cgaggcgatc cttcgcgctg ctggtgccag
     2281 tggttcaagg tcaccaacgc cgagtgggag tcgaccgagc cggcgcagtt cgagcagatg
     2341 ctctgcgacc aggtgagcgc cgccaccgtg tcacccgggc tgatcgccta cgccgacggt
     2401 gagccggcgg gatgggccgc cgtcgaggcc cgccccggct accgccgact gaccaccatg
     2461 aaggtcgctg cggcgagccc ggagcctcag ggcgacccgg cggtctgggc cgtgacatgc
     2521 ttcgtcgtgc gtgtcgggta ccgcaagcgc ggcgtctccg ccgccctgct ggccggggcg
     2581 atggagttcg cccgctcgca cggtgcgcgc ctcctcgagg cctatccggt ggatgtcgcg
     2641 gagaagaaga cctcgtcggc ggacctgttc cacggcgccc tgtcgacctt cctctcggcc
     2701 gggttcgagg tggtggcccg cccctcagac ggccgtgcgg tggtgcagct ggcgctgcag
     2761 tagtacgacg gcggcggcgg cgagggccag cgcggcgatc accagcgtgg ccgtggcggt
     2821 cgcggcatcc ggggccacga tggactggcc gcgcagggcc tgccaggtca gcaccgcgag
     2881 caccgcgacg taggcggcgc tgacgatcgc gacgaggcgg tagcgcacat ccacgtcgcg
     2941 cagcacggcg atgcgtcgcg cggcgagctc gatgaggatg agcgcgagcg ggatcgcctg
     3001 cagggcgtgc atcccgatga agtggggaat gcgcaggtcg ccggctaccg tgctccaccc
     3061 cagcagcggc aggccgggac caccatcggc gagcccgacg gtgtgggcgc cggcgatgcc
     3121 ctgccagtcg tcgagctggg cggcggtcgg gccggtcatg aggaacgcga gcgccatgcc
     3181 tgcgagcgcg atgagtgcgc cggcgcggat ggcgagggtg cgtgcgcgat cgcccagcga
     3241 gttgcggaac aggatgatgc tgaccacgaa cgtcgcgacc cacagcgtgc cgatcgaggc
     3301 ggccatgatc gaccacagca cagtgttgaa cggcgtggag acgttgaagt gactcgtgat
     3361 gcccgccgcg gcagcgccgg tgatgatcac gagttcgatc agcaggagta tcgcgctgat
     3421 cgtgccggcc caccacgcca ccctctggaa acgctgcagc tggccgatca gccacgacca
     3481 ggtcaccgcg tagacggcgg tcgagatcgc gaacttgagg ggcttctccc acacgttgac
     3541 gccgagcgat tcgcgcgggt cgaccacgag gccgacgagg cacacgacga cgagcacgcc
     3601 catggcggcc gcgagcacca gcagggggcg gtgccagcgg atgcccgcga cagtcgacgt
     3661 cggacggttc tgaactgctg tggtcatcgt tcactcccgg agggttcggc cccgcgcggt
     3721 gcggggaaca cttggtacga cacgtgctcc tgcgccatgc ggcgcaggga tgcgttgagg
     3781 gcgtcaccga gcacggtgcc gacgacgacg gtctcggcca tgtcggcgac atccgcgtgg
     3841 tgcgcgacgg cggcgagatc ggcgcgggcg atgagttcgg ccgcttccgc gtaggcgggc
     3901 aggtgctcgc cgatgtgctt ctggccgagg tcggcgtagg tgtcgagaac gcgagccgcg
     3961 ccgcggcggc cggggttctc gtcggagacg agccatcccg tgctctcgat gaggtcgttg
     4021 acctgggtcg tgccggcact gccctcgggt acatcggcgt agaggctcgt gtcggagatc
     4081 gcgtactggg cgacaccgaa gacgtagctg agcggaaggt cgggcgagtc gatcgcctcg
     4141 agcacgcgct gcgctgtagc gaccgagagt ccaccgatgt cgatgagcgc ccggatcagg
     4201 cgcagccgct cgacgtgctc ggggccgtac tccgcctggg tggcgctggt gcgctcgccc
     4261 gcgggcagga gcccttcgcg caggtagaac ttgatcgagg gcacggatac cccgctcgcc
     4321 tcgcacagct gcgacatctt catgttggat agcgtagcta tcgatagtgc tgctgtctag
     4381 tgcgaatgcg cgctgcgggc aacaaaaagc ccccgtcgcc gggggcttca tgtggtgctt
     4441 gctaaagctg gggtacctgg actcgaacca agaacaaaag aatcagaatc ttccgtgttg
//
LOCUS       DAOSOU010000180         4496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_19385_length_4496_cov_5.771448, whole genome shotgun sequence.
ACCESSION   DAOSOU010000180 DAOSOU010000000
VERSION     DAOSOU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19385_length_4496_cov_5.771448"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLW15_09300"
     CDS             <1..734
                     /locus_tag="PLW15_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOT34627.1"
                     /translation="LAILVEQMRREGYELTVGKPQVVTKYIDGKLNEPFEHLTVDSPE
                     EHLGAITQLMAARKGRMETMTNHGTGWVRMEFIVPSRGLIGFRTEFLTITRGTGIANA
                     ISHGYEPWAGAIETRTNGSIVADRSGVATPFAMTALQERMTFFVEPTQEVYEGMVVGE
                     NSRADDMDVNITKEKQLTNMRQSTSDVFERMTPSRKLTLEECLEFAREDECVEVTPEV
                     IRIRKVELDANARARAASQRKKQNA"
     gene            824..1351
                     /locus_tag="PLW15_09305"
     CDS             824..1351
                     /locus_tag="PLW15_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34628.1"
                     /translation="MTAHEIRQRPRRRPVVLVGLVGLGLAASLSLTGCFAAPTITGGG
                     TGGAGQSGQSGDGTVTDGGTTTSPPDSGLSDFAGLPAGFPTDEIPLIAGDVAFGVDLG
                     TGWTVVIPVADGASAFADASARLTGAGYESLAEQTQPEGSFGVFQNDKYQVQLTIADT
                     NDFGLAATYVAALRG"
     gene            1390..1857
                     /locus_tag="PLW15_09310"
     CDS             1390..1857
                     /locus_tag="PLW15_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34629.1"
                     /translation="MSTLRNRVTVPIAIALALVITPALSGCFGNPIESLIEGATGGDV
                     DLGGTSLPDGYPSSEVPVIDGQILFGGSIGNDQGKVYNVTVKVADAGALDTIKAQLEG
                     VGFSSEAGFGGSTADGGTYIGQTDAWGVLVVVSKDGENGFVANYTVTSADAAQ"
     gene            complement(1859..3148)
                     /gene="efeB"
                     /locus_tag="PLW15_09315"
     CDS             complement(1859..3148)
                     /gene="efeB"
                     /locus_tag="PLW15_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HOT34630.1"
                     /translation="MSDTNDPQPGLSRRGLLGFLGAGAVGVGAVATGAAATTTRSPAF
                     AAPVAHSASAAQSGAAAIYPFHGEHQSGIVTPAQDRLHFAAFDVSDVTRAELQELLQD
                     WSYAASRMMAGLSAGDLGAVGGAYDAPPDDTGEALDLPPAGLTITFGFGPSLFDDRFG
                     LASRRPAALIDLPHFPGDALVETLTGGDLCIQACSDDPQVAVHAIRNLSRIAFGRASL
                     RWSQLGFGRTSSTTRAQVTPRNLFGFKDGTANLRAEDTALVDTHVWADGDDWMTGGSY
                     LVARKIRMTIETWDRTSLREQETVVGRTKGDGAPLSGGGEFTEPDFTATGRDDAPLIA
                     ETSHVRLAHPSMNNGATLLRRGYNFVDGNDELGRLNAGLFFICFQADPRTQFVPVQLS
                     LARHDAMNEYLRHVGSGIFAVPPGAAQDSYVGAGLFA"
     gene            complement(3148..4326)
                     /locus_tag="PLW15_09320"
     CDS             complement(3148..4326)
                     /locus_tag="PLW15_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M75 family protein"
                     /protein_id="HOT34631.1"
                     /translation="MIPRSAPATVSLTALGLAALVALTGCVPNATSAAGSTELTVDSS
                     ADACTVSAAEAPSGTISFVVTNSGDQVTEFYLLGDDGLRIVGEAENIGPGISRTLVVQ
                     ASAGDYFTVCKPGMVGAGVGKAAFTVTDSGTPLTLGDDLSAQIDTANANYKAYVKDQV
                     ENLVTGTEAFAAAYIAGDDDAARAEYAPTRVFWERIETVAESFGDLDPRMDLREADLE
                     EGQDWTGWHAIEKDLWPAEAEPGFQPYDQTKREALATQLVDDTHTLYADVEQLEFTLA
                     QQTNGAIGLLDEVASGKVTGEEEFWSHTDLWDFQANIDGARVLYEGVRDILTVKDAAL
                     ADRLDAEFDALQVLLDEQRVGDGFRLYTELEAAQVRGLSDQVNALAEPLSKLTAALVL
                     "
     gene            complement(4326..>4496)
                     /locus_tag="PLW15_09325"
     CDS             complement(4326..>4496)
                     /locus_tag="PLW15_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity Fe2+/Pb2+ permease"
                     /protein_id="HOT34632.1"
                     /translation="WYGTLLKGTLNFSPATTWLEAIVWVAYVVPVMTIFILKSRQRPS
                     VPVAPTPETAGA"
BASE COUNT          736 a         1500 c         1577 g          683 t
ORIGIN      
        1 ccctggcgat cctcgtcgag cagatgcgtc gcgagggcta cgagctcacc gtcggcaagc
       61 cgcaggtggt caccaagtac atcgacggca agctcaacga gccgttcgag cacctgactg
      121 tcgactcgcc cgaggagcac ctgggtgcga tcacccagct catggccgcc cgcaagggtc
      181 gcatggagac gatgacgaac cacggcaccg gctgggtgcg catggagttc atcgtcccgt
      241 cacgtggcct catcggcttc cgtaccgaat tcctcacgat cacgcgcggc accggaatcg
      301 ccaacgcgat ctcgcacggc tacgagccgt gggcgggcgc gatcgagacc cgcaccaacg
      361 gatccatcgt ggccgaccgc tcgggcgtgg cgacaccgtt cgcgatgacc gcactgcagg
      421 agcgcatgac gttcttcgtc gagccgacgc aggaggtcta cgagggcatg gtcgtgggcg
      481 agaactcgcg ggccgacgac atggacgtca acatcacgaa ggagaagcag ctgaccaaca
      541 tgcgtcagtc gacctccgac gtgttcgagc gcatgacccc gtcacgcaag ctgaccctcg
      601 aggagtgcct cgagttcgcc cgcgaggacg agtgcgtcga ggtgaccccc gaggtcatcc
      661 gcatccgcaa ggtcgagctc gacgccaacg cgcgtgcccg cgccgcctcg cagcgcaaga
      721 agcagaacgc ctaactgtca acccgcagcg ggggttcgcg cgtcgtagag acatgacgaa
      781 acccccactg cgcgacagtc cgctcaccgg ttacgctggg cgcgtgacag cacacgagat
      841 acgtcagcgc ccacggcgcc ggccggttgt tctggtcgga ctcgtgggac tcgggcttgc
      901 ggcatccctg tctctgaccg gatgcttcgc cgcgcccacc atcaccggtg gcggcaccgg
      961 cggcgccggc cagtccggcc agagtggcga cggcaccgtg accgacggcg gcacgaccac
     1021 ctcgccgccc gacagcgggc tttctgactt cgcgggccta cccgccgggt tcccgaccga
     1081 cgagatcccg ctcattgccg gtgacgtggc attcggcgtc gatctgggca cgggctggac
     1141 cgtggtcatc cccgtagccg acggagcctc ggcgttcgct gacgccagcg ctcggctcac
     1201 cggtgccggg tacgagagcc tcgccgagca gacccagccc gagggatcgt tcggggtctt
     1261 ccagaacgac aagtaccagg tgcagctgac catcgcagac accaacgact tcggactcgc
     1321 agccacctat gtggccgcgc tacgaggcta gatcccgctt cacccctatc gagacagagg
     1381 agtaacccca tgagcacact gcgtaaccgt gtcacagtcc ccatcgcgat cgccctcgcg
     1441 ctggtcatca caccggcact gtccggttgt ttcggcaacc cgatcgagag cctcatcgaa
     1501 ggggcgaccg gcggcgacgt cgacctcggc ggcaccagcc tgcccgacgg ctacccgagc
     1561 agcgaggtgc ccgtcatcga cggccagatc ctcttcggcg ggtcgatcgg caacgatcag
     1621 ggcaaggtct acaacgtgac ggtcaaggtc gccgacgctg gcgctctcga cacgatcaag
     1681 gcacagctcg agggcgtcgg attcagcagc gaggcaggct tcggcggatc gaccgctgac
     1741 ggaggcacct acatcggcca gaccgatgcc tggggcgttc tcgttgtcgt gtccaaggac
     1801 ggcgagaacg gcttcgtggc caactacacc gtgacgtcgg cggacgccgc gcagtaggtc
     1861 aggcgaacag cccggcgccc acgtaggagt cctgcgcggc gccgggcggc accgcgaaga
     1921 tgcctgagcc gacgtggcgc aggtactcgt tcatggcgtc gtgtcgagcc agcgagagct
     1981 gcacgggcac gaactgcgtg cgcgggtcgg cctggaagca gatgaagaac aggccggcgt
     2041 tcaggcggcc gagctcgtcg ttgccgtcga cgaagttgta gccgcgccgc agcagcgtcg
     2101 cgccgttgtt catcgacggg tgcgcgaggc gcacgtgcga cgtctcggcg atgagcgggg
     2161 cgtcatcccg tcccgtcgcg gtgaagtcgg gctcggtgaa ctcgccgcct ccactgagcg
     2221 gcgcgccgtc gcccttggtg cgtccgacca cggtctcctg ctcgcgcagt gacgtgcggt
     2281 cccaggtctc gatggtcatg cggatcttgc gcgcgacgag gtacgagccg ccggtcatcc
     2341 agtcgtcgcc gtcggcccag acgtgagtat cgacgagcgc ggtgtcctcg gcgcggaggt
     2401 tggcggtgcc gtccttgaag ccgaacaggt tgcgcggggt gacctgggca cgagtcgtcg
     2461 acgacgtgcg cccgaagccg agctgcgacc atcgcaggga tgcccgcccg aacgctatgc
     2521 gcgacaggtt cctgatcgcg tgcacggcca cctgcgggtc atccgagcag gcctgaatgc
     2581 agaggtcgcc gccggtgagg gtctcgacga gggcatcgcc cggaaagtgc ggcaggtcga
     2641 tgagcgccgc cgggcggcgc gaggcgagcc cgaagcggtc gtcgaacaga ctcgggccga
     2701 agccgaacgt gatggtgagt cccgcgggcg gaaggtcgag tgcctcaccg gtgtcgtcgg
     2761 gcggggcgtc gtaggcgcca ccgacagcgc cgaggtcgcc ggccgacagg ccagccatca
     2821 tgcgggatgc cgcgtagctc cagtcctgca gcagctcctg cagctcggct cgcgtcacgt
     2881 cgctcacgtc gaacgcggcg aagtgcagcc tgtcttgtgc gggcgtcacg atgccggact
     2941 ggtgctcgcc gtggaacggg tagatggcgg cggcgccgga ctgggccgcc gacgccgagt
     3001 gagccaccgg cgctgcgaag gcgggggagc gggtggtcgt cgcggcagca cccgtcgcca
     3061 ccgctccgac tccgacagcg ccggcaccga ggaatccgag caggccgcga cgggacagcc
     3121 ccggctgcgg gtcgttcgtg tcactcatca gagcacgagg gcggcggtca gcttcgacag
     3181 cggctcggcc agggcattga cctggtcgga caggccgcgg acctgcgccg cctcgagctc
     3241 ggtgtagagg cggaatccgt caccgacgcg ctgctcgtcg aggagcacct gcagcgcgtc
     3301 gaactcggcg tcgagacggt cggcgagtgc ggcatccttg accgtcagga tgtcgcgcac
     3361 gccctcgtac agcaccctcg cgccgtcgat gttggcctgg aagtcccaga ggtcggtgtg
     3421 cgaccagaac tcctcctcgc ccgtcacctt gccgctggcg acctcgtcga gaagcccgat
     3481 cgcgccgttg gtctgctgcg cgagcgtgaa ctcgagctgc tctacgtcgg cgtacagggt
     3541 gtgggtgtca tcgacgagct gggtggccag tgcctcgcgc ttggtctggt cgtagggctg
     3601 gaacccgggt tcggcctcgg cgggccagag atccttctcg atggcgtgcc agccggtcca
     3661 gtcctggccc tcctcgaggt ccgcctcgcg caggtccatg cgcgggtcga ggtcgccgaa
     3721 cgattcggcc acggtctcga tgcgctccca gaagacccgg gtcggcgcgt actcagcgcg
     3781 cgcggcgtca tcgtcacccg cgatataggc ggcggcgaac gcctcggtgc ccgtgacgag
     3841 gttctcgacc tggtccttga cgtaggcctt gtagttggcg ttggcggtgt cgatctgggc
     3901 gctgaggtcg tcaccgagcg tgaggggggt tcccgagtcg gtgaccgtga aggcagcctt
     3961 gccgacgccc gcgccgacca ttccgggctt gcagacggtg aagtagtccc cggcactggc
     4021 ctgcaccacg agggtgcgcg agatgccggg accgatgttc tcggcctcgc cgacgatgcg
     4081 cagaccgtcg tcgcccagca ggtagaactc ggtcacctgg tcgccggagt tggtcacgac
     4141 gaacgagatg gtgccactcg gagcctcggc cgccgacacg gtgcaggcgt cggcgctcga
     4201 gtccactgtc agctcggtcg acccggcggc ggatgtcgcg ttcggcacgc atccggtgag
     4261 cgcgaccagc gcggcaagac ccaacgcggt gagactcaca gttgcggggg cagaacgggg
     4321 gatcatcagg ctccagcggt ttcgggggtg ggtgcaaccg gcaccgaggg gcgctggcgg
     4381 gacttgagga tgaagatcgt catgaccggt acgacgtagg cgacccacac gatcgcctcc
     4441 agccaggtgg tcgcggggga gaagttgagc gtgcccttga gcagcgttcc gtacca
//
LOCUS       DAOSOU010000181         4482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_19472_length_4482_cov_7.118365, whole genome shotgun sequence.
ACCESSION   DAOSOU010000181 DAOSOU010000000
VERSION     DAOSOU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19472_length_4482_cov_7.118365"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLW15_09330"
     CDS             complement(<1..874)
                     /locus_tag="PLW15_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT34633.1"
                     /translation="MATRTKAPAAAASVKAPAAKAAPTKASAAKASTAVDAPELTRAE
                     KAAATKAAKAAAASAAPVKAPATKRGSKATAPADPEDGNDDVDEVVEIDVDDDAIDPE
                     EIDVVVAVVEVEVDVDADSDDAKDDDDDDDEKSTGKDEELPTGALVLSLVDDEDEVPV
                     YSSAITGATADPVKDYLKQIGKVALLNAAEEVELAMRIEAGLFAEDKLSHMSAAEQKS
                     QLGRELLWVAKDGQRAKSHLLGANLRLVVSLAKRYTGRGMQFLDLIQEGNLGLIRAVE
                     KFDYTKGFKFSTYAT"
     gene            complement(1061..2386)
                     /locus_tag="PLW15_09335"
     CDS             complement(1061..2386)
                     /locus_tag="PLW15_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34634.1"
                     /translation="MNSRRAWLMFSIAIAAYMVAVLQRSSLGVAAVEATDRFSVNAAA
                     LSTLAVVQIVVYAALQIPVGVMLDRVGPRVLLTLGAALMALGQSTLAVAPTLGVALIG
                     RILVGAGDAMTFISAVRFLTNWFDGKTIPIVTQLLGTAGQLGQVLSALPLALALHAWG
                     WTPTYLSAAALSVIVVIVLGVLARGRGPNELPLAQQSWAASGHQLRESLRRPGTQLGF
                     WTHFVAGGSVTMFSLLWGFPFLSIALGYGPTGAAGLLTIMIASAAVSGPILGLLSARF
                     PLRRSNIVLGIVTMLAALWGATLAWPATPPLWLIVLLLVFIAASGPGSLIGFDFARTF
                     NPQRAHGSASGVVNVGGFTSGFVLMLIVGILLDAMDRTRGGSGIPAELYSLESFRLAF
                     LAQYAVIGAGVGFMLVARRRTRRRLHAEEGIEVAPIWVSLMRAWRHRFR"
     gene            complement(2405..3331)
                     /locus_tag="PLW15_09340"
     CDS             complement(2405..3331)
                     /locus_tag="PLW15_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOT34635.1"
                     /translation="MRDPGGLYDISTDVIEVPSGLHLVAGLTGFADAGSAVSQVSEYL
                     LDTLEHSTVLTFDNDELLDYRARRPTMYFDQDHLSDYQPAALKLYLARDELGQQFLLL
                     TGYEPDFQWERFTAAVLDIVQRYGVSTTTWINSIPMPVPHTRSIGVTVSGNRSELTDA
                     MSVWKPRTQVPATALHLIEFRLRELAHPTTGFVLLIPHYLADTEFPLAAVAALESVSA
                     ATGLIFPTDRLREEGRDFLAKIDAQVENNQELAKLVGTLEERHDSYMQDNPLRSPLTD
                     VDGELPTADEIAAELQNFLAARRNGDDDTPLQ"
     gene            3422..>4482
                     /locus_tag="PLW15_09345"
     CDS             3422..>4482
                     /locus_tag="PLW15_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOT34636.1"
                     /translation="MTVPALSVSPEPPLTIAADVLVLGVSKTDDGPRLLSDAAELAEL
                     QLVLASIGVTGAQDELRRIPGSYGATESIALIGVGSGEVTVDDLRYAAGSAARQLRGV
                     ESIVFALPVKDDADTLAVLEGAAIGAYAYTEYRSSSLESTKLPATTVIVAAAGSDALV
                     SKASIIAEAVHTVRDLVNMPPSDLYPESFAAAARALAEPTVVTVDVLDEKQLAAGGFG
                     GILGVGQGSTRPPRLVKAVYAPKGATRHLALVGKGITFDSGGLSLKPPASMIGMKYDM
                     TGAATVLAVVLAAAKLELDVKVTAWLCIAENMPSGSALRPNDVIRIRGGKTVEVLNTD
                     AEGRLVLADGLVAASEEEP"
BASE COUNT          773 a         1473 c         1493 g          743 t
ORIGIN      
        1 acgttgcgta ggtggagaac ttgaagccct tggtgtagtc gaacttctcg acggcacgga
       61 tgagacccag gttgccctcc tggatcaggt cgaggaactg catgccgcgg ccggtgtagc
      121 gcttcgcaag cgagacgacg aggcgcaggt tggcgccgag caggtggctc ttcgcccgct
      181 gcccgtcctt ggcgacccag agcagttcgc gtccgagttg cgacttctgc tcggccgctg
      241 acatgtgcga gagcttgtcc tcggcaaaga ggcccgcctc gatgcgcatg gcgagctcga
      301 cctcctcggc agcattgagc agtgcgacct tgccgatctg cttcaggtag tccttgacgg
      361 ggtctgccgt agcgccggtg atcgccgacg aatagacagg gacctcgtcc tcgtcgtcga
      421 ccagcgacag caccagggcg ccggtcggca gttcctcgtc cttgcccgtc gacttctcgt
      481 catcgtcatc gtcgtcgtcc ttggcgtcat ccgagtccgc gtcgacatct acctcgactt
      541 cgaccacggc gacaacgaca tcgatctcct cggggtcgat cgcgtcgtcg tcaacatcga
      601 tctccacgac ctcatcgacg tcgtcattgc cgtcttcggg gtctgcgggg gccgttgcct
      661 tcgagccgcg cttggtcgcc ggagccttca ctggggcggc cgatgccgcc gcggccttcg
      721 ccgccttcgt ggcagcggcc ttctcagcgc gggtgagctc gggtgcgtcg acggcggtgg
      781 acgccttcgc ggcggaagcc ttcgtgggtg cggccttcgc cgcaggggcc ttgacgctcg
      841 cagctgcggc gggggccttg gttcgggttg ccatgtttcg aatgcctttc gatcgggcag
      901 cactgttttt gggcagtact aagacccatg tcaagtcgtg attcgttgcg ctcgacgagc
      961 ttgaacatcc agggatgcca cgaatcccga aactagaccg ggtctcgaat caattattgc
     1021 acggtttgat tggaccaacc gccagagtcc gggagggcct tcagcggaac cggtgccgcc
     1081 acgccctcat gagtgaaacc cagatcggtg ccacttctat tccctcctcg gcgtgcagcc
     1141 gacgtcgggt gcgtcggcgg gcgacgagca tgaagccgac tccggcgccg atgaccgcgt
     1201 actgggcgag gaaggcgagc cggaaggatt ccagcgaata gagctcggcc gggatccctg
     1261 atccgccgcg ggtccgatcc atcgcgtcga gcaggattcc gacgatgagc atcagaacga
     1321 aaccggaggt gaatccgccc acgttgacca ctccactggc agagccgtga gcccgttggg
     1381 gattgaacgt gcgcgcgaag tcgaagccga tcagtgatcc gggaccgctc gcggcgatga
     1441 acaccagcag gagcacgatc agccagagtg gaggggttgc cggccaggcg agagtcgctc
     1501 cccacagcgc tgcgagcatg gtcacgatcc ccaggacgat gttgcttcgc cgcaggggga
     1561 atcgtgccga gagcagcccg agtatcggcc ccgatacggc cgcgctcgcg atcatgatcg
     1621 tcagaagccc cgccgctccg gtcggcccgt agcccagcgc gatcgagagg aacggaaacc
     1681 cccagagcag cgagaacatc gtcacggatc cgccggcgac gaagtgcgtc cagaacccca
     1741 gctgcgtgcc cggtctgcgc agactctccc gcagttgatg accggatgcc gcccaactct
     1801 gctgcgcgag tggcagctca ttcggacctc ggccgcgggc caagaccccg agcacgatga
     1861 ccacgatcac tgacagagcg gcggcgctca ggtaggtcgg ggtccatccc cacgcgtgca
     1921 gtgccaatgc caggggaagt gccgacagca cctggccgag ctgtccagcg gtgcccagca
     1981 actgggtcac gatcggaatg gtcttgccgt cgaaccagtt ggtgagaaac cgcacggcgg
     2041 agatgaaggt catggcgtcc ccggcaccca ccaggatgcg gccgatgagc gcaacgccga
     2101 gagtgggcgc caccgcgaga gtgctctgcc ccagcgccat caacgcggcc ccgagcgtga
     2161 gcagcacgcg cggcccgacc ctgtcgagca tgactcccac cggaatctga agagccgcgt
     2221 agaccacgat ctgcaccaca gcgagcgtcg agagcgcggc ggcgttcacg ctgaagcgat
     2281 cggttgcctc gactgcggcc acacccagcg atgatcgctg cagaacggcc accatgtatg
     2341 cggcgatggc gatggagaac atcagccacg cgcgacggga gttcatcgct cccatgctac
     2401 gggcctactg gagcggggtg tcgtcgtctc cgttgcgccg cgcagcgagg aagttctgca
     2461 gctcggcggc gatctcatcc gcggtaggca gctcgccgtc gacgtcggtc agcggggatc
     2521 gcagcgggtt gtcctgcatg tagctgtcgt gacgctcctc gagcgtgccg acgagcttcg
     2581 cgagctcctg gttgttctcc acctgggcgt cgatcttcgc gaggaagtcg cgcccctctt
     2641 cgcgcaggcg atccgtcggg aagatcagtc cggttgccgc actgacgctc tccaacgcgg
     2701 ccaccgcggc gagggggaac tcggtgtcgg cgaggtagtg cggaatgagg agcacgaagc
     2761 cggtcgtcgg gtgggccaac tcccggagtc ggaactcgat caggtgcaac gcggtcgccg
     2821 gcacttgcgt gcgcggcttc cacaccgaca tcgcatcggt gagctcggaa cggttgccgc
     2881 tcaccgtgac accgatggag cgcgtgtgcg gaacaggcat gggaatcgag ttgatccacg
     2941 tggtcgtgct gaccccgtag cgctgcacga tatccagcac ggcagcagtg aatcgctccc
     3001 actggaagtc gggctcgtag ccggtcagca gcaggaactg ctgcccgagc tcgtcgcgcg
     3061 ccaagtagag cttcagagcc gcgggctggt agtcgctcag atgatcctga tcgaagtaca
     3121 tcgtggggcg ccgcgcccgg tagtcgagca gttcgtcgtt gtcgaaggtc agcacagtgc
     3181 tgtgctcgag ggtgtcgagc aggtactcgc tgacctggct cacagcgctt cccgcatccg
     3241 caaaaccggt gagacccgcg acgagatgca accccgatgg cacctcgatg acatctgtcg
     3301 agatgtcgta caggccgccc ggatcgcgca tgcaacaact ctaatctcgg cgacgcacgg
     3361 catgctccgc gcgcgtgatg ccctgagcga acagcacagg cggcctcgct acgatggggc
     3421 catgaccgtg cccgctctgt ctgtgagccc agaaccgccc ctgaccattg ccgccgacgt
     3481 gctcgtgctc ggagtgagca agaccgacga cggaccccga ctcctgagcg atgccgccga
     3541 gctcgccgag ctccagctgg tgctcgcgtc gatcggcgtg accggcgcgc aggacgagct
     3601 caggcgcatc ccgggcagct acggagccac cgagagcatc gcgctcatcg gcgtcggcag
     3661 cggcgaggtc accgtcgacg acctgcggta tgccgcagga tcagcagccc ggcagctgcg
     3721 cggagtcgag tcgatcgtgt tcgcgctgcc cgtgaaggat gacgccgaca ccctcgccgt
     3781 gctcgagggg gctgcgatcg gcgcctacgc gtacaccgag taccgctcca gctcgctcga
     3841 gtcgaccaag ctgccggcga ccacggtgat cgtggcggcc gccggctccg acgctctggt
     3901 atcgaaggca tcgatcatcg ccgaggcggt gcacaccgtg cgggacctcg tcaacatgcc
     3961 tccgtccgac ctctatcccg agtcgttcgc ggccgcggcc cgggcgctgg ccgagcccac
     4021 ggtcgtcact gtcgacgtcc tggacgagaa gcaactcgcc gcgggtggat tcggcgggat
     4081 tctcggcgtc ggccagggct cgactcgccc gccacgactc gtgaaggcgg tctacgcacc
     4141 gaagggcgcg acccgccacc tggctctcgt gggcaaggga atcacgttcg actcgggcgg
     4201 gctctcgctg aagcccccgg catccatgat cggcatgaag tacgacatga ctggagcggc
     4261 aacagtgctc gcggtcgttc ttgccgcggc caagctggag ctcgacgtca aggtcacggc
     4321 gtggctctgc atcgcggaga acatgccttc gggttcggcg ctgcgcccca atgacgtcat
     4381 ccgcatccgg gggggcaaga ccgtcgaggt gctcaacact gacgcggaag gccggctcgt
     4441 gctcgctgac ggccttgtcg ccgcgagcga ggaggagccc ga
//
LOCUS       DAOSOU010000182         4467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_19551_length_4467_cov_5.647325, whole genome shotgun sequence.
ACCESSION   DAOSOU010000182 DAOSOU010000000
VERSION     DAOSOU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19551_length_4467_cov_5.647325"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLW15_09350"
     CDS             <1..313
                     /locus_tag="PLW15_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOT34637.1"
                     /translation="LGIVEHSLEERRTGIEGWFDTPSSIEVISPTGAVPPRWKQMVSI
                     FLVFFPLSLLSNWALSPITGAWPLPLRVLVTVLLVTPVMTYVAMPFVTRALRPWLTRG
                     L"
     gene            complement(310..1041)
                     /gene="budA"
                     /locus_tag="PLW15_09355"
     CDS             complement(310..1041)
                     /gene="budA"
                     /locus_tag="PLW15_09355"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOT34638.1"
                     /translation="MTDDLSPRPSGVLHQFSFVDALVAGLYEGAFRASDVLGAGDFGV
                     GCGDALDGEIVLLDGVMHLCRGDGQVVDVAPDALLPFAEVSRFTPTHTETVADLTELD
                     FDALVDSLTPSSNLFYGIRVDGVFDRMTVREAVRQQQPYRGLAEAVKGQHEDTSAHTT
                     GTLIGFRGPDVFQGLSVAEFHLHYINDARTFGGHVMDFQLRSGMLALEAYDTFTLHLP
                     EVASYLSAPLRRHDSDAEIRQAEGS"
     gene            complement(1045..1422)
                     /gene="rsfS"
                     /locus_tag="PLW15_09360"
     CDS             complement(1045..1422)
                     /gene="rsfS"
                     /locus_tag="PLW15_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT34639.1"
                     /translation="MTASARARELLTVMALAADSKQGEDLVALDVSGPLPLTDIFFLA
                     TGRNERNVQAIATEIEDKMIEAGAKPLRREGRAEGRWILLDFGDVVAHVFHEEDRLYY
                     SLERLWKDCPAIALDLPAPRTAS"
     gene            complement(1464..2978)
                     /locus_tag="PLW15_09365"
     CDS             complement(1464..2978)
                     /locus_tag="PLW15_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34640.1"
                     /translation="MTTYQDPQPQSRRAVRQGERGETPEQQEQVAFPGQTPPQFYPDS
                     VNAREMWDTTARRAAQLPPAAAPRPDAPAATSGRRSAQPAPSAAEPLSYSTQGLPPAQ
                     QANPTPSFRPRGSSQAPEVRAEQPSATHAAPPITPAQPEPGAFRVRDFSPEGRRSAAP
                     QAHVDSPVEAQQLPASDLDYHTEARVDRAAPPAPPIAEVPPFVSQPQPVAPELIQPPV
                     SQAPVSHAPVSHAPVVQTPVDLIPPSAPGVPVEQTLTRREMRALQHSEGDAAREEPVW
                     GQPEASSPVQSPFPPAPEPDVNTALTNAITEFDQLFQAQPTAQAQPTPQVQAQPTAQA
                     QPTPQADAATSTWTAPVGHWSVQADLDDDEFHETTINRTVGSGSTATSALVLPTIPGG
                     HDIRGPLTGTGEIMLTGSIELPHTLSSTGATARFDNGGIDALFDAGDAEVISTDSAPV
                     RAIKAVSTHNSGQGVTHTQKPKGTRTLTVLLIAASSMAVVVAGLLITAFALNVF"
     gene            complement(2975..3577)
                     /gene="nadD"
                     /locus_tag="PLW15_09370"
     CDS             complement(2975..3577)
                     /gene="nadD"
                     /locus_tag="PLW15_09370"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT34641.1"
                     /translation="MAEPAQRRARIGVMGGTFDPIHNGHLVAASEVQQQFELDEVIFV
                     PTGEPWMKPGVTEGEHRYLMTVIATAANPRFNVSRVDIDRDGPTYTVDTLRDIRKARP
                     DADLFFITGADAVAQILDWKDVDEVWNLAHFIAVSRPGHTLTIRGLPEQGVSSLEVPA
                     LAISSTDCRSRVSRGFPVWYLVPDGVVQYIAKHHLYRSST"
     gene            complement(3592..3783)
                     /locus_tag="PLW15_09375"
     CDS             complement(3592..3783)
                     /locus_tag="PLW15_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34642.1"
                     /translation="MSLIALALAETEELAPLIMPPLAFAAIAVVVFVALAIVSWSFRD
                     VSNRHTKNPNGSNTHGAGH"
     gene            complement(3813..>4467)
                     /locus_tag="PLW15_09380"
     CDS             complement(3813..>4467)
                     /locus_tag="PLW15_09380"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOT34643.1"
                     /translation="LIPRGSADLINSVVAESTVPVIETGAGVVHIYLDESADLENSVA
                     IVHNAKVQRPSVCNALETLLVNAASAPRLIPPVLAKLHEAGVTVHGDSRTLELFPDAL
                     PATDEDWSTEYMSLDVAVRVVDDLDDALDHIRQYSTHHTESIITNDLARAERFLAEVD
                     SAVVMVNASTRFTDGGEFGFGAEVGISTQKLHARGPMGLPELTSTKWVVRGSGQVRG"
BASE COUNT          722 a         1413 c         1584 g          748 t
ORIGIN      
        1 gctggggatc gtggagcact ccctggagga acgccgcacc ggcatcgagg gctggttcga
       61 caccccctcg agcatcgagg tgatctcgcc cacgggagcc gtgccgccgc gctggaagca
      121 gatggtgtcg atcttcctcg tgttcttccc gctcagcctt ctctccaatt gggccctgtc
      181 gccgatcacc ggtgcgtggc cactcccgct gcgcgtgctc gtcaccgtgc tactggtcac
      241 gccggtcatg acctacgtgg ccatgccgtt cgtcacgcga gccctgcgac cctggctgac
      301 gcgcggactc taggagccct cggcctggcg gatctccgcg tccgagtcgt gccgacgcag
      361 gggcgctgac aggtacgagg cgacctccgg caggtgcagc gtgaaggtgt cgtaggcctc
      421 gagcgcgagc atcccggatc tgagctggaa gtccatgacg tggcccccga aggtgcgggc
      481 atcgttgatg tagtgcaggt ggaactcggc gacgctgagg ccctggaata catccgggcc
      541 gcggaacccg atcagggtgc cggtggtgtg cgcggaggtg tcctcgtgct gccccttcac
      601 ggcctcggcg aggccgcgat agggctgctg ctggcgcacg gcctcgcgca ccgtcatccg
      661 atcgaagacg ccgtcgacgc ggatgccgta gaagaggtta ctgctgggcg tgagtgaatc
      721 gacgagcgcg tcgaagtcga gctctgtcag gtcggcgacc gtctcggtgt gggtcggggt
      781 gaaccgcgac acctcggcga acggcagcag ggcatcgggc gcgacatcca cgacctgccc
      841 gtcgcctcga cagaggtgca tgacgccgtc gagcagcacg atctccccgt cgagtgcgtc
      901 gccgcagccc accccgaagt cgccagcgcc cagcacatcg gatgcccgga aggcgccctc
      961 gtagagacct gcgaccagcg catccacgaa cgagaactgg tgcagcacgc ccgacggccg
     1021 cggactcagg tcgtcggtca tgacctagct cgcggtgcgg ggcgccggca gatcgagagc
     1081 gatcgccggg cagtccttcc acagacgctc aagcgagtag tacaggcggt cctcctcgtg
     1141 gaagacgtgc gcgaccacgt cgccgaagtc gagcagaatc caccgaccct cggcgcgtcc
     1201 ctcccggcgc agcggcttgg cgccggcctc gatcatcttg tcctcgatct cggtggcgat
     1261 ggcctgaacg ttgcgctcgt tgcgacccgt agcgaggaag aagatgtcgg tgaggggcag
     1321 aggacccgac acgtcgagtg cgaccagatc ctcgccctgc ttggagtcgg ccgcaagggc
     1381 catcacggtc agaagctcgc gggcgcgcgc agatgcagtc acagggtcct caggaagtag
     1441 gagaaaggaa gtgtcgtcaa ggatcagaac acgttgaggg cgaaagccgt gatgagcagc
     1501 cccgcgacga cgacggccat ggatgacgcg gcgatgagca gcaccgtgag ggtgcgcgtg
     1561 cccttcggct tctgggtgtg agtcacaccc tgaccggagt tgtgggtgct caccgccttg
     1621 atcgcccgca cgggtgccga gtcagtggag atgacctcgg cgtcccccgc gtcgaacagt
     1681 gcgtcgattc cgccattgtc gaaccgtgcc gtcgcgccgg ttgagctcag ggtgtgcgga
     1741 agctcgatcg agccggtgag catgatctct ccagtgccgg tcaacggtcc gcggatgtcg
     1801 tgtccaccgg gaatcgtggg aagcaccagg gcgctcgtgg ccgtgctgcc gctgccgacg
     1861 gtgcgattga tcgtggtctc gtggaactcg tcgtcatcga ggtcggcctg aaccgaccag
     1921 tggccgacgg gtgccgtcca ggtggatgtc gcggcatcgg cctgaggcgt cggctgcgcc
     1981 tgggcggttg gctgcgcctg aacctgcggc gttggctgcg cctgggcggt cggctgcgcc
     2041 tggaacagct ggtcgaactc ggtgatcgcg ttcgtgagcg ccgtgttgac gtcgggctcc
     2101 ggcgcgggag ggaacggcga ctgaacgggc gagctggcct cgggctggcc ccagacgggc
     2161 tcctcccgtg ccgcatcccc ctcggagtgc tgtagcgcgc gcatctcgcg gcgagtcaga
     2221 gtctgttcca caggtacgcc gggggcgctc ggtgggatga ggtccaccgg tgtttggact
     2281 accggtgcgt gggataccgg ggcgtgggat accggggctt ggctcaccgg cggctgaatc
     2341 agttcgggtg cgaccggctg cggctgggac acgaacggcg gaacctctgc gatcggcggt
     2401 gccggcggag cagcccgatc aacccgggct tccgtgtggt agtcgagatc gctcgcgggc
     2461 agctgctggg cctcaaccgg cgaatcgaca tgagcctgcg gggcagccga cctgcggcct
     2521 tccgggctga agtcgcgcac gcggaatgct cccggctccg gctgagcggg ggtgatcgga
     2581 ggggcggcgt gggtggcaga gggctgctcg gcgcgaactt ccggcgcctg tgaggaacca
     2641 cgaggacgga acgagggcgt cgggttcgcc tgctgggccg ggggcaggcc ctgggtggaa
     2701 taggacagcg gttctgcggc cgacggagcc ggctgggccg agcgtcgccc ggatgttgcg
     2761 gccggggcat ccggtcgagg ggccgccgcg ggcggcaact gtgcggcgcg acgcgcggtg
     2821 gtatcccaca tctcgcgggc gttgacggaa tcggggtaga actgcggcgg agtctggccc
     2881 gggaacgcta cctgctcctg ctgctcggga gtctcgccgc gctcaccctg ccgcaccgca
     2941 cgccgcgact gcggctgcgg atcttggtac gtggtcatgt gctactccga tacaagtggt
     3001 gcttggcgat gtactgaacg acaccatcgg gcaccaaata ccaaaccgga aacccccgac
     3061 tcaccctgct tcgacagtcc gtggaggata tcgccaacgc cggcacttca agcgagctta
     3121 cgccttgctc tggcaaaccg cgaatagtga gggtgtgtcc tggccgcgaa acggctatga
     3181 aatgcgcgag attccagacc tcatcgacgt ccttccagtc gagaatctgc gccaccgcgt
     3241 cggcgccggt gatgaagaac aggtcggcat ccggacgggc tttgcggatg tcacgaagcg
     3301 tgtcgacggt gtacgtcggg ccatccctgt cgatgtcgac gcggctcaca ttgaaccgcg
     3361 gattggcggc ggtcgcgatg accgtcatga ggtagcgatg ctccccctcg gtgactccgg
     3421 gcttcatcca cggctcaccc gtgggaacga agatcacctc gtcgagttcg aactgctgct
     3481 gaacctcgct cgccgcgacc aagtggccgt tgtgaatcgg gtcgaaggtg ccacccatga
     3541 caccgatgcg ggcacgtcgc tgtgctggct cggccatgcg ggcaggagaa cctagtggcc
     3601 cgcgccgtgc gtgttggagc cgttcgggtt cttcgtgtga cggttggaca cgtcacggaa
     3661 gctccacgac acgatcgcca gcgcgacgaa caccacgacg gcaatcgccg cgaaggccag
     3721 cggaggcatg atcaagggag caagctcctc cgtctcagcg agtgcgaggg cgatcagcga
     3781 catcatttct ccgttcgggg tgtagcccta tcctatccgc gcacctggcc cgacccgcgc
     3841 acaacccact tggtgctggt cagttcgggc agacccatcg gccctcgggc gtggagcttc
     3901 tgagtcgaga taccgacttc ggcaccgaac ccgaactcac cgccgtcggt gaacctcgta
     3961 gaggcgttga ccatgacgac ggcggagtcg acctcggcga ggaatcgttc cgcgcgcgcg
     4021 agatcgttgg tgatgatcga ctcggtgtga tgggtcgagt actggcggat gtgatcgagc
     4081 gcgtcatcca ggtcgtcgac gacccgcacg gccacgtcga ggctcatgta ctcggtcgac
     4141 cagtcctcgt cggtcgcggg cagcgcgtcg gggaacagct cgagggtgcg cgagtcgccg
     4201 tgcacagtga cccccgcctc atgcagcttg gcgagcaccg gcggaatcaa ccggggggcg
     4261 gatgccgcgt tcacgagcag agtctccagt gcgttgcaga cgctgggccg ctgcaccttc
     4321 gcgttgtgga cgatcgccac cgagttctcg aggtcggcgg actcgtcgag gtagatgtgc
     4381 acgacacccg caccggtctc gatcaccgga accgtcgact cggcgaccac cgagttgatg
     4441 aggtcggcgc tgccgcgcgg gatgagc
//
LOCUS       DAOSOU010000183         4390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_19983_length_4390_cov_5.508189, whole genome shotgun sequence.
ACCESSION   DAOSOU010000183 DAOSOU010000000
VERSION     DAOSOU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19983_length_4390_cov_5.508189"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..785)
                     /gene="mtrA"
                     /locus_tag="PLW15_09385"
     CDS             complement(105..785)
                     /gene="mtrA"
                     /locus_tag="PLW15_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HOT34644.1"
                     /translation="MNARILVVDDDTALAEMIGIVLRTEGFEPHFCEDGGTAVDAFRS
                     AKPDLVLLDLMLPGLSGIDVCARIREESGVPIIMLTAKSDTADVVKGLESGADDYVVK
                     PFNPKELVARIRTRLRPTPETSTGTLTVGDLTVDVAGHEVKRGDTKINLTPLEFELLL
                     ALALKPQQVFTREMLLEQVWGYHYKADTRLVNVHVQRLRAKVEEDPDNPQIVMTVRGV
                     GYRAGSTP"
     gene            910..2097
                     /locus_tag="PLW15_09390"
     CDS             910..2097
                     /locus_tag="PLW15_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34645.1"
                     /translation="MTDSSPWQPPTDVTGSAASAAGPFESPTGVPAPPAGPQAGWTPP
                     PKPGLIPLRPLTLGTLLGASFQVLRRNPRPMFGFALLVTGVVFFASFLLVGLVALFAF
                     SRAASATGADSATVSAGSVALVVLAGLISVALSLVASAILQGVVSLEVARGTVGEKLR
                     LGGLWRAARGRIGALIGWTLLITAAVIVVILLLVLIIGLIAAFGGVVGAVIAVLLGIA
                     AFFGAIVLGAWLATRLSLVPSAIMIERLPLLAAIGRSWQLTIGFFWKTLGIQLLVAVI
                     VSFVTNIVSTPLQLVIGLGSSLINPNDQQSAALIGIVVAYILTVVLTVVVGAIGSVMQ
                     SATAALIYIDLRMRKEGLDLELQRFVEARQAGDTSVGDPYAPPAGIVNPPSASTPPPA
                     SAQ"
     gene            2097..2735
                     /locus_tag="PLW15_09395"
     CDS             2097..2735
                     /locus_tag="PLW15_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOT34646.1"
                     /translation="MLPWDVPVDPEPPEARQWLVDELSKPQYQAAKPTLFDQIAKAIS
                     DWFSSLQIGTVEGPPAFGLGVILVLVAAALVVAFLIFGVPRLNRRSRVAGVLFGEDDD
                     RGSADMRRDAAAAASRGDYATAIAELFRALARGLAERTIVTVTPGTTARGFAGRAGVT
                     FPQFADRLVTAAEAFDDVRYLGHPGSVEGYEALVALESEVRAARPALEPVPA"
     gene            2732..3919
                     /locus_tag="PLW15_09400"
     CDS             2732..3919
                     /locus_tag="PLW15_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOT34647.1"
                     /translation="MTDQQSTDTTVLTPRARTVLRRWLFWIGAGLVALVIGVLAFAFA
                     GTAAAGGYLDPESAAPQGSMAVAEVLRQQGVTVVVTTSLEETQQAIQSPDATTLFVYD
                     TDQLLEASQWRTVAGLTHRVIIADAGFVTLRVAAPALAQAGVVDGTLRADCDLPVVQR
                     ADTVSGSGSGFRVVDDAAAATACLGSGDGVFSLIEVPRGQGTVAILGATDALTNEQVV
                     DNGNAAFALGLLGGDETLVWYLPSFADVESTGPATLGELTPLWVTPALSLLVIAVIAA
                     AVWRGRRLGPLVIENLPVTVRASETMQGRARLYERSNARLRALDALRVGSVQRLAATC
                     GLSRLASVDDVIGAVAAATGGQVGTIRRLLVDDIPNTDRDLLALSDALLTLERDVTRA
                     LRP"
     gene            3967..>4390
                     /locus_tag="PLW15_09405"
     CDS             3967..>4390
                     /locus_tag="PLW15_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34648.1"
                     /translation="MTDTQLRDAFAKLRTEVGKAVVGQDAAVTGLTIALLAGGHVLLE
                     GVPGVAKTLLVRTLSHTLKLDTKRVQFTPDLMPGDLTGSLVYDSKAGEFEFREGPVFT
                     NILLADEINRTPPKTQSALLEAMEERQVSVDGATRKLPV"
BASE COUNT          613 a         1562 c         1455 g          760 t
ORIGIN      
        1 ccgccgagag cagcacggtg atcgcgaccg tgcgcacctg gagggaggac cgccagaggc
       61 gacccagctt gtccagccag gcgccagtcc agatcgggca tggtctaggg ggtgctgccc
      121 gcgcgatagc ccacgccacg cacggtcatg acgatctggg ggttgtcggg gtcctcctcg
      181 accttcgcgc gaaggcgctg cacgtgaaca ttcacgagac gggtgtcggc cttgtagtgg
      241 taaccccaga cctgctcgag cagcatctcc cgggtgaaga cctgctgggg cttcagggcg
      301 agggcgagca gcaactcgaa ctccagcggc gtgaggttga tcttggtgtc gccgcgcttg
      361 acctcgtggc cggcgacatc cacggtgagg tcacccacgg tgagggtgcc ggtggacgtc
      421 tcgggcgtcg gccgcagccg ggtgcggatg cgcgccacca actccttggg attgaacggc
      481 ttgacgacgt agtcgtcggc gccgctctcg agccctttga cgacgtcggc cgtgtcgctc
      541 ttggcggtga gcatgatgat cggaacaccg gattcctcgc ggatgcgagc acagacgtcg
      601 attccgctca ggccgggcag catgaggtcc aggagcacca gatcgggctt tgccgagcgg
      661 aacgcgtcga ccgccgtacc gccgtcctcg cagaagtgcg gctcgaagcc ttccgtgcgc
      721 agcacgattc cgatcatctc cgcgagcgcg gtgtcgtcat cgactacaag aattcgcgcg
      781 ttcactggtc ctcgcaactt cctcgatccc gcttgtattc actctgcggg agctttcgca
      841 caagagtagt cgagtgtgaa agcatgattg cgatcgatcc tcgaactaga gcttcagaga
      901 ggccctgacg tgaccgacag ttccccgtgg cagccgccca ccgatgtgac cggttctgca
      961 gcgtccgccg caggcccgtt cgagtccccg accggcgttc ccgctccacc ggcgggcccg
     1021 caggcgggct ggacgccgcc gccgaagccc ggactcatcc cgctgcgacc gctcaccctc
     1081 ggcacactgc tgggcgcgtc gttccaggtg ctgcggcgca acccgcgccc gatgttcggg
     1141 ttcgcgctgc tcgtgaccgg tgtggtgttc ttcgcatcgt tccttctcgt ggggctcgtc
     1201 gccctcttcg ccttctcgcg tgccgccagt gccaccgggg ctgattcggc caccgtctct
     1261 gccggatcgg tcgcgctcgt ggtgctcgct ggcctcatca gcgtcgcgct gtcgctggtc
     1321 gcctccgcca tcctccaggg cgtcgtctcg ctcgaggtcg cgcggggaac ggtcggcgag
     1381 aagctgcgac tcggcgggct gtggcgggcg gcgcgcggcc gcatcggcgc cctcatcggc
     1441 tggacgctgc tgatcaccgc cgcggtcatc gtcgtgatcc tgctgctcgt gctcatcatc
     1501 ggcctcatcg ccgccttcgg cggtgtcgtc ggcgccgtga tcgccgtgct gctcggcatc
     1561 gccgcgttct tcggtgccat cgtgctcggt gcctggttgg cgacccgcct gtcgctggtg
     1621 cccagcgcga tcatgatcga gcgcctgccg ctgctggcgg cgatcgggcg ttcgtggcag
     1681 ctgacgatcg gattcttctg gaagaccctg ggcatccagt tgctggtcgc tgtcatcgtc
     1741 tcgttcgtga ccaacatcgt ctcgacgccg ctgcagctcg tcatcgggct cggcagttcc
     1801 ctgatcaacc ccaacgatca gcagagcgcg gccctgatcg gcatcgtcgt cgcgtacatc
     1861 ctcacggtcg ttctgacggt cgtggtcgga gcgatcggct cggtcatgca gtcggcgacc
     1921 gccgcgctca tctacatcga cctgcgcatg cgcaaagagg ggctcgacct cgaactccag
     1981 cgcttcgtcg aggcgcgcca ggccggcgac acctccgtgg gcgacccgta tgcgccgccc
     2041 gccggcatcg tgaatccacc gtcagcatcg acacccccgc cagcatcggc acagtgatgc
     2101 tgccctggga cgttcccgtc gaccccgaac cgcccgaggc ccggcagtgg ctggtcgacg
     2161 agttgtcgaa gccccagtac caggccgcca agcccaccct gttcgatcag atcgcgaagg
     2221 cgatctccga ctggttctcg tcgctgcaga tcggaaccgt cgaaggcccg cccgccttcg
     2281 gtctcggcgt gatcctggtg ctcgtggctg ccgcgctggt ggtggccttc ctgatcttcg
     2341 gggtgccgcg gctgaaccgc cgatcccggg tcgctggggt gctcttcggc gaggacgacg
     2401 atcgcggctc ggcagacatg cgacgggatg ctgcggccgc ggcatcccga ggcgactacg
     2461 ccacggcgat cgccgagctg ttccgtgctc tcgcacgcgg actggccgag cgcaccatcg
     2521 tcaccgtgac gccgggcacc accgctcggg gattcgccgg ccgggccggt gtcacgttcc
     2581 cgcagttcgc cgatcgactc gtgacggcgg ccgaggcgtt cgacgacgtg cgctacctcg
     2641 gccaccccgg ttcggtcgaa gggtatgagg cattggtggc gctcgagtcg gaggttcgtg
     2701 ccgcccgccc cgccctggag ccggtgccgg cgtgaccgat cagcagtcca ccgataccac
     2761 cgtgctgacc ccgcgtgcgc gcacggtgct gcgccgctgg ctgttctgga tcggcgcagg
     2821 gctcgtcgca ctggtgatcg gcgttctcgc cttcgcgttc gcgggaaccg cagcagcggg
     2881 cggctacctc gaccccgaga gtgcggcacc ccaggggtcg atggccgtcg ccgaggtgct
     2941 ccgtcagcag ggggtcaccg tcgtggtcac gaccagcctg gaggagaccc agcaggccat
     3001 ccagtcgccc gatgcgacca cgctgttcgt ctacgacacc gaccagttgc tcgaggcctc
     3061 gcagtggcgc accgtggcgg ggctcaccca ccgggtcatc atcgcggatg ccggcttcgt
     3121 caccctccgt gttgccgccc ccgccctcgc tcaggccgga gtggtcgacg gcacgctgcg
     3181 cgccgactgc gacctgcccg tcgtgcagcg cgccgacacc gtgagcggct cgggctccgg
     3241 cttccgggtg gtcgacgacg cggcagcggc cacagcctgc ctcggcagcg gtgacggcgt
     3301 gttctcgctc attgaggtgc cgcgaggtca gggcacggtc gcgatcctgg gtgccaccga
     3361 cgcactgacc aacgaacagg tcgtcgacaa cggcaatgcc gccttcgcgc tgggcctgct
     3421 cggcggggac gagaccctcg tctggtacct gccgagcttc gccgacgtgg aatcgaccgg
     3481 ccctgccacc ctcggcgagc tcaccccgct ctgggtgacg cccgctctca gcctgctcgt
     3541 catcgccgtc atcgcggcgg ccgtgtggcg cggtcggcgg ctcggccccc tcgtcatcga
     3601 gaaccttccg gtgaccgtgc gggcgagcga gaccatgcag ggtcgcgctc ggctctacga
     3661 acgctcgaac gcacggttgc gcgctctgga tgcgctgcgc gtcggctcgg tgcagcgact
     3721 ggccgccacc tgcgggctgt cccgtctcgc cagcgtcgat gacgtgatcg gtgcggtagc
     3781 tgcggcgacc ggggggcagg tgggtacaat ccgtcgtctg ctggtcgacg acatcccgaa
     3841 caccgaccgt gacctgctcg cactctcgga tgccctgctc accctcgagc gcgacgtcac
     3901 ccgagcgctg cgcccctgac cccgaccgct acacaaccgc ccaaccgaat aggacagaat
     3961 cgttccatga ctgacactca actgcgcgac gcgttcgcga agctgcgcac cgaggtgggc
     4021 aaggccgtcg tcggacagga tgccgcagtc accggcctca ccatcgccct gctcgcgggc
     4081 gggcacgtgc tgctcgaggg cgtgcccggc gtcgccaaga cgttgctcgt gcgcaccctc
     4141 agccacacgc tcaagctcga caccaagcgg gtgcagttca cgcccgacct gatgcccggt
     4201 gacctcaccg gctcgctggt gtacgactcc aaggcgggtg agttcgagtt ccgcgagggg
     4261 ccggtgttca ccaacatcct gctcgccgac gagatcaacc gcacaccgcc caagacccag
     4321 tcggccctcc tggaggccat ggaggagcgc caggtctcgg tcgacggagc cactcgcaaa
     4381 cttcccgtgc
//
LOCUS       DAOSOU010000184         4382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20034_length_4382_cov_5.303675, whole genome shotgun sequence.
ACCESSION   DAOSOU010000184 DAOSOU010000000
VERSION     DAOSOU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20034_length_4382_cov_5.303675"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="sufB"
                     /locus_tag="PLW15_09410"
     CDS             <1..742
                     /gene="sufB"
                     /locus_tag="PLW15_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOT34649.1"
                     /translation="DEGSYVHYIEGCTAPIYKSDSLHSAVVEIIVKKNARVRYTTIQN
                     WSNNVYNLVTKRAIAHEGATMEWIDGNIGSKVTMKYPSIYLVGEHAKGETLSVAFAGP
                     GQHQDAGAKMIHMAPYTTSSIVSKSIARGGGRAGYRGEVRVDEKAHHSANTVRCDALL
                     VDTISRSDTYPAIDIRVDDVQLGHEATVSRVSAEQLFYLQSRGMPEDEAMAMIVRGFI
                     EPIARELPMEYALELNKLIEMSMEGSVG"
     gene            743..1924
                     /gene="sufD"
                     /locus_tag="PLW15_09415"
     CDS             743..1924
                     /gene="sufD"
                     /locus_tag="PLW15_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOT34650.1"
                     /translation="MSLATTEQHGAKAHSDGGWDDTSRVPVQTRSERFTSVDPAEFPA
                     VTGLEVDWKLTPVAQVRDLIDGELDGSGYDLAPQLAEGGTIEWITRADPRVGSAGSPE
                     DKASANAWSSFDTALAITITGDAPVALGFDRSGFGSAPKAAHTVITAKTNSTGHIVLR
                     STGPANLVENIEIVVEDGAQLTVVSLQEWDDDAHHLASHFAQVGRDATLTHIVVTLGG
                     RVVRVNPSLHLSGEGSDGAMYGLYFADAGQHLEQRVYLHHQAANTKGRVNYKGALQGT
                     GARTVWVGDVLIGREASGTDSYEQNRNLVLSEGTRADSIPNLEIETGDILGAGHASAT
                     GRFDDEQLFYLQARGISEAEARRLVVIGFLAEIVQKIGDPALEEYLQGRIESELSGAG
                     S"
     gene            1921..2253
                     /locus_tag="PLW15_09420"
     CDS             1921..2253
                     /locus_tag="PLW15_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOT34651.1"
                     /translation="MTAERIGTTAELEVNKAHRVVLSGVPIVVVKDAAGVVYAIGDTC
                     THGDISLAEGFVEDDAIECWAHGSMFSLTTGKPLTLPAYEPVPVYAVSVDADGVITID
                     PNEKVAVS"
     gene            2255..3019
                     /gene="sufC"
                     /locus_tag="PLW15_09425"
     CDS             2255..3019
                     /gene="sufC"
                     /locus_tag="PLW15_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOT34652.1"
                     /translation="MSVLEVKDLHVSVETEQGTKQILKGVDLTVKQGEIHAIMGPNGS
                     GKSTLAYSIAGHPKYHVESGSVLLDGAEVLTMSVDERARAGIFLAMQYPVEIPGVRMT
                     DFLRTAKTAIDGEAPAIRSWIKEVNEAMKSLRMDKSFGERNVNEGFSGGEKKRHEILQ
                     LELLKPKFAVLDETDSGLDVDALKIVSEGVNRAHEANDLGVLLITHYTRILRYIKPGF
                     VHVFVDGRIAEEGGPELAERLENEGYDRFLAPSEVA"
     gene            3024..3353
                     /locus_tag="PLW15_09430"
     CDS             3024..3353
                     /locus_tag="PLW15_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOT34653.1"
                     /translation="MATALAPALFDQVEEALKDVVDPELGVNIVDLGLIYDLSWDDEN
                     NALIISMTLTSAGCPLTDVLEEQTAQSLDGIVEAFRINWVWMPPWGPEKITDDGREMM
                     RALGFSI"
     gene            complement(3415..4044)
                     /locus_tag="PLW15_09435"
     CDS             complement(3415..4044)
                     /locus_tag="PLW15_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOT34654.1"
                     /translation="MSSITLAVGRPTLADRIFTRTLALDIVLVVAGAALTAVAAQVAV
                     PLWPVPITGQTLAVLLVGVTLGATRGAISLALYAVLGIVGLPVFSEHSSGWGVIAGPT
                     GGYIIGFIFAAALTGWLAQLQWDRKILRAFLAFLAGSVVPFAFGLPWLAAALGSLGFP
                     NDLNSVLQAGLYPFILGGVIKAALGAGIITLAWYAVGVSDKRKAAKSAE"
     gene            4162..>4382
                     /locus_tag="PLW15_09440"
     CDS             4162..>4382
                     /locus_tag="PLW15_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT34655.1"
                     /translation="MLSVHELELRVGARLLMEGVSFRVDRGDKIGLVGRNGAGKTTLT
                     KVLAGELPPTDGKIERGGAIGYLPQDPRSG"
BASE COUNT          845 a         1432 c         1439 g          666 t
ORIGIN      
        1 cgacgagggc tcgtacgtgc actacatcga gggctgcacc gcgccgatct acaagagcga
       61 ctcgctgcac tccgccgtcg tggagatcat cgtcaagaag aacgcccgcg tccggtacac
      121 cacgatccag aactggtcga acaacgtcta caacctcgtg accaagcgcg cgatcgcgca
      181 cgagggcgcc accatggagt ggatcgacgg caacatcggc tccaaggtga cgatgaagta
      241 tccgtccatc tatctggtgg gggagcacgc caagggcgag acgctctcgg tcgcgttcgc
      301 gggtcccggt cagcaccagg acgctggcgc caagatgatc cacatggcgc cgtacacgac
      361 gtcatccatc gtctccaagt ccatcgcccg cggtggcggt cgcgccggtt accggggcga
      421 ggtgcgcgtc gacgagaagg cgcaccactc cgccaatacc gtgcgctgcg acgccctgct
      481 ggtcgacacg atctcgcgat cggataccta tcccgccatc gacatccgcg tggatgacgt
      541 gcagctcggc cacgaggcga cggtctcccg ggtcagcgcc gagcagctgt tctacctgca
      601 gtcccgcggc atgcccgagg acgaagcaat ggccatgatc gtgcgcggct tcatcgagcc
      661 gatcgcccgc gagctgccca tggagtacgc gctcgaactc aacaagctca tcgagatgag
      721 catggaaggc agtgtcggct agatgagtct ggcaacaacc gaacagcacg gggcgaaggc
      781 ccacagcgac ggcggctggg atgacacgag ccgcgtgccg gtgcagaccc gctccgagcg
      841 cttcacctcc gtcgaccctg ccgagtttcc cgcagtcacc ggactcgagg tcgactggaa
      901 gctgacgccc gtagcgcagg tgcgcgacct catcgacggc gaactggatg gctcggggta
      961 cgacctcgcg ccccagcttg ccgagggcgg cacgatcgag tggatcacgc gcgccgatcc
     1021 ccgggtcggc tcggccggat cacccgagga caaggcgtcg gcgaacgcct ggtcgagctt
     1081 cgacaccgca ctcgcgatca cgatcaccgg cgatgccccc gtggcgctcg gcttcgaccg
     1141 cagcggcttc ggctccgccc cgaaagcggc ccacaccgtg atcacggcga agacgaacag
     1201 caccggtcac atcgtcctgc gcagcaccgg gccggcgaac ctcgtcgaga acatcgagat
     1261 cgtcgtcgaa gacggcgctc agctgacggt cgtctcactc caggagtggg atgacgatgc
     1321 gcatcacctc gcgagccact tcgcccaggt gggacgggac gcaaccctga cccacatcgt
     1381 cgtcactctc ggcggaagag tggtgcgcgt caacccgtcg ctgcacctct cgggcgaagg
     1441 atccgacggc gcgatgtacg ggctctactt cgccgacgcc ggccagcacc tcgagcagcg
     1501 cgtctacctc caccaccagg cggcgaacac gaagggccgg gtcaactaca agggtgcgct
     1561 ccagggcacc ggcgcccgca cggtgtgggt gggcgacgtg ctcatcggcc gcgaggcatc
     1621 cggcaccgat tcctacgagc agaaccgcaa cctcgtgctc tcggagggca cccgcgcgga
     1681 ttcgatcccg aacctggaga tcgagaccgg ggacatcctc ggtgccggcc acgccagcgc
     1741 gaccgggcgg ttcgacgacg agcagctctt ctacctgcag gctcgcggaa tctccgaggc
     1801 cgaggcccgc cgactcgtcg tgatcggctt cctcgccgag atcgtgcaga agatcggcga
     1861 ccccgcactc gaggagtacc tgcagggcag gatcgaatca gaactgtcag gagcaggatc
     1921 atgaccgccg aacgcatcgg gaccaccgcc gaactcgagg tgaacaaggc ccatcgggtc
     1981 gtgctgtcgg gcgtccccat cgtcgtcgtc aaggatgccg cgggcgtcgt gtacgccatc
     2041 ggtgacacct gcactcacgg cgacatctcc cttgcagaag gttttgtcga ggatgacgcg
     2101 atcgagtgct gggcgcacgg atcgatgttc tcgctgacca ccggaaagcc cctcacgctt
     2161 cccgcctacg aaccagttcc tgtctacgcc gtgagcgttg acgccgatgg cgtgatcacc
     2221 atagatccga atgagaaagt cgcagtctcc taacatgtca gtactcgaag tcaaggatct
     2281 ccacgtcagc gtcgagaccg agcagggcac caagcagatc ctcaagggtg tcgacctcac
     2341 tgtgaagcag ggcgagatcc acgccatcat gggtccgaac ggatcgggca agtcgacgct
     2401 cgcctattcg atcgccgggc atccgaagta tcacgtcgag agcggatcgg tgctgctgga
     2461 tggtgccgag gtgctgacga tgagcgttga cgagcgtgcc cgggccggga tcttcctcgc
     2521 gatgcagtac cccgttgaga tccccggagt gcgcatgacc gacttcctgc gcacggccaa
     2581 gaccgccatc gacggcgagg cgcccgcgat ccgctcctgg atcaaggagg tcaacgaagc
     2641 catgaagtcc ctgcgcatgg acaagagctt cggtgagcgc aacgtcaacg agggcttctc
     2701 cggcggagag aagaagcgcc acgagatcct tcagctcgag ctgctcaagc ccaagttcgc
     2761 agtgctcgac gagaccgact ccggcctcga cgtggacgcg ctcaagatcg tctctgaggg
     2821 cgtcaaccgc gcccacgagg cgaacgacct gggcgtgctc ctgatcaccc actacacgcg
     2881 catcctgcgc tacatcaagc cgggcttcgt gcacgtcttc gtcgacggcc gcatcgcaga
     2941 agagggcggg ccggagcttg ccgaacgtct cgagaacgag gggtacgatc gctttctcgc
     3001 gccctcggaa gtagcgtaga agcatggcca ccgcactcgc accggcactc ttcgaccagg
     3061 tcgaggaggc gctcaaggat gtcgtcgacc ccgagctcgg cgtcaatatc gtcgacctcg
     3121 gtctgatcta cgacctgagt tgggatgacg agaacaacgc gctcatcatc agcatgaccc
     3181 tgaccagcgc gggatgcccg ctgaccgacg tgctcgagga gcagactgca cagtctctgg
     3241 acggaatcgt ggaggcattc cgcatcaact gggtgtggat gccgccgtgg ggtcccgaga
     3301 agatcaccga tgacggccgc gagatgatgc gcgcgctcgg cttctcgatc tgagactcgc
     3361 tcttaacgcg gaatggcccg gactcaggtc cgggccattg ttgttctgcg agatctactc
     3421 ggcggacttc gccgccttgc gcttgtcgga gacaccgacg gcgtaccagg ccagggtgat
     3481 gatgccggcg ccgagcgcgg ccttgatcac gccaccgagg atgaacgggt agagaccggc
     3541 ctgcagcacc gagttgaggt cgttggggaa tccgagggat ccgagcgctg ccgccagcca
     3601 cggcaggccg aaggcgaacg gcacgaccga tcccgcgagg aaggcgagga aggcgcgcag
     3661 gatcttgcgg tcccactgga gctgcgcgag ccatccggtc agagctgcgg cgaagatgaa
     3721 gccgatgatg tagccaccgg tcgggcctgc gatgacgccc cagccggagg agtgctcgct
     3781 gaacacgggg agcccgacga tgccgagcac cgcgtagagg gcgagggaga tggcaccgcg
     3841 ggtcgccccg agcgtgacgc cgacgagcag cacggcgagg gtctgaccgg tgatcggcac
     3901 cggccagagc ggcacagcca cctgagcggc gacggcggtc agcgccgcac cggcgacaac
     3961 aagcacgatg tcgagggcaa gggtgcgggt gaagatgcgg tcggcgagag tcgggcgacc
     4021 gacggccaga gtgatgctgc tcatggggct cctcgtgtga aggggatgcg gggcagtctg
     4081 ccacggcgca caaagtagac tatctggctg acccccctta tctgcgttgt attcccgcgc
     4141 cctaagaact ggacattttc tgtgctttct gtgcacgagc tcgagctgcg tgtcggtgca
     4201 cgcctcctca tggagggcgt gtcgttccgc gtcgaccgcg gcgacaagat cgggctcgtg
     4261 ggtcgcaatg gcgcaggcaa gaccacgctc accaaggtgc tcgccggcga gctgccgccc
     4321 accgacggca agatcgagcg tggcggcgca atcgggtacc tgccgcagga cccgcggtcg
     4381 gg
//
LOCUS       DAOSOU010000185         4353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20197_length_4353_cov_5.000698, whole genome shotgun sequence.
ACCESSION   DAOSOU010000185 DAOSOU010000000
VERSION     DAOSOU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20197_length_4353_cov_5.000698"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLW15_09445"
     CDS             <1..389
                     /locus_tag="PLW15_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOT34656.1"
                     /translation="LRANEARYYKNKYDHDFAVAPAADEPETLAYVTRVLAEERDIVI
                     ASPPLEVTMFEVEDLRMAYVFYQSGLAINVMYGLEPGGKRAVGFKLSEGMDVPEELET
                     RFKFARQKSKLAGVIRGSFFVIKGEY"
     gene            460..588
                     /locus_tag="PLW15_09450"
     CDS             460..588
                     /locus_tag="PLW15_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34657.1"
                     /translation="MSPQHEPSDLMSAEAELHRIDHTATIWFAAALIAVALPSRVS"
     gene            654..815
                     /locus_tag="PLW15_09455"
     CDS             654..815
                     /locus_tag="PLW15_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34658.1"
                     /translation="MGIIGLSAIGYAGCPIYWGSVSTAHLQKSIAIRAGLAMLLIGSF
                     VAISAVLGG"
     gene            complement(823..1347)
                     /locus_tag="PLW15_09460"
     CDS             complement(823..1347)
                     /locus_tag="PLW15_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOT34659.1"
                     /translation="MSTRILFVRAVNVGGATLPMAEFRTLLAELGGGSVRTYIASGNA
                     VVDVPGDAARFDRAVEAAITERYGFVREVISRTPAEVSAALAAHPFEVVDPKFSYITF
                     LTATPAADALDAAANVPTGDDEWQLAGRELHLRFAGGMGRAVLNLDRLLRSLGVAGTA
                     RNLRTTQALVELAG"
     gene            complement(1344..2426)
                     /locus_tag="PLW15_09465"
     CDS             complement(1344..2426)
                     /locus_tag="PLW15_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT34660.1"
                     /translation="MPATLPIGTVVDSGNIAELNAQRFNRHTFWCGQSGSGKTYALGV
                     VLEQILAHTSLPLVILDPNADFVRLGEVRPEAASTGTAAALAGRDIRVLRPGDRDDSL
                     RVRFSALPFPIKAALLRLDPLDDRAEYNALLHLQERYPINDERSIVERLRESGDPVHH
                     ALAERVENLALDDWGLWAGERAAATEIIETRPDATVLDLGGFRYAEEPLVVALSVLDD
                     LWTRREQRRPVLIVIDEAHNLCSPDLDSPLGRAVREQIIRIAAEGRKYGLWLLLSTQR
                     PSKVHQGIISQCDNLALMKMSSPIDLAELASIFGFVPESLIEQSPLFTQGMALFAGGF
                     IGAPTIVQMGDRVTFEGGSDVGVPLR"
     gene            2517..2592
                     /locus_tag="PLW15_09470"
     tRNA            2517..2592
                     /locus_tag="PLW15_09470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2550..2552,aa:Arg,seq:acg)
     gene            3901..>4353
                     /locus_tag="PLW15_09475"
     CDS             3901..>4353
                     /locus_tag="PLW15_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34661.1"
                     /translation="MTATAVQALGAVAAITAGLAWSGGVRPAYLVPLLATFLILTIVG
                     ERLELARVGSPGERAEGALLGFAFVLAAATVLTLTVPVIAVPVAGVALLGIVVWLARY
                     DIARMTVRQTGLPRFVAVGLLVGFAWLAVAGAGWILGGARTEGPVYDAV"
BASE COUNT          684 a         1404 c         1478 g          787 t
ORIGIN      
        1 gcctgcgcgc caacgaagct cggtactaca agaacaagta cgaccacgac ttcgccgtcg
       61 cgcccgcagc cgacgagccc gagaccctcg cctacgtcac gcgcgttctg gccgaggagc
      121 gcgacatcgt gatcgcctca ccgcccctcg aggtcacgat gttcgaggtc gaggacctgc
      181 gcatggccta cgtgttctac cagtcggggc tggcgatcaa cgtgatgtac ggcctcgaac
      241 ccggcggcaa gcgggctgtc ggcttcaagc tgtccgaggg catggatgtt cctgaggagc
      301 tcgagacccg gttcaagttc gcgcgacaga agtcgaagct ggccggcgtc atccgtgggt
      361 cgttcttcgt catcaagggc gagtactgac tcgggcttga tcgacgcggc actcggcacg
      421 aggtaaagcc cgcccgcccg ccggggttag cctgtggaca tgagccccca gcatgagccc
      481 agtgacctca tgtccgccga ggccgaactg catcgcatcg accacacggc gaccatctgg
      541 ttcgctgccg ctctcatcgc cgtcgcgctg ccctcacggg tctcctgatc tccggatggc
      601 gcccctccga cctcacgccc gttgccgccg ccttctggtg ggccggcgcc atcgtgggca
      661 tcatcggact gtcggcgatc ggctacgcgg gctgcccgat ctactggggc agcgtgtcga
      721 ccgcccactt gcagaagtcg atcgcgatcc gcgcgggact cgcaatgctg ctgatcggat
      781 cgttcgttgc gatctcggcg gtgctcggag gctgacggcc gcttagccgg cgagctccac
      841 gagcgcttgc gtggtgcgca ggttgcgcgc cgtgccggcc actcccagcg atctgagcag
      901 gcgatcgagg ttcagcactg cgcggcccat gccgccggcg aacctcaggt ggagctcgcg
      961 cccggcgagc tgccactcgt cgtcgccggt ggggacgttc gccgcggcat ccagagcgtc
     1021 ggccgctggc gtcgcagtga ggaaggtgat gtacgagaac ttcgggtcga cgacctcgaa
     1081 cggatgcgcc gcgagcgccg ccgatacctc cgccggtgtg cgggagatca cctcgcgcac
     1141 gaagccgtac ctctccgtga tcgccgcctc gacggcgcga tcgaaccgtg cggcgtcgcc
     1201 cgggacatcg accacggcat tgcccgacgc gatgtaggtg cgcacgctgc cgccgccgag
     1261 ctcggccagc agcgtacgga attccgccat gggcagggtc gcacctccga cgttcaccgc
     1321 acgcacgaac aggatgcgtg tgctcaccgc agcggcacgc ccacgtcgct gccgccctcg
     1381 aaggtgacgc ggtcgcccat ctgcacgatc gtcggggctc cgatgaaccc gcccgcgaac
     1441 agtgccatgc cctgcgtgaa caggggcgac tgctcgatga gcgattcggg cacaaagccg
     1501 aagatcgaag cgagctcggc gaggtcgatc ggtgaactca tcttcatcag ggcgaggttg
     1561 tcgcactggg agatgatgcc ctggtgcacc ttcgacgggc gttgtgttga cagcagcagc
     1621 cacagcccgt acttgcggcc ctccgccgcg atccggatga tctgctcgcg caccgcgcgg
     1681 ccgagtgggc tgtcgaggtc gggggagcag aggttgtgcg cctcgtcgat gacgatgagc
     1741 acgggtcggc gttgctcgcg gcgggtccat aggtcgtcga gtaccgagag cgcgacgacg
     1801 agaggctcct cggcgtagcg gaatccgccg aggtcgagca ccgtggcatc cggtcgggtc
     1861 tcgatgatct cggtggcggc ggcccgctcg ccggcccaga gcccccagtc gtcgagcgcg
     1921 aggttctcga cgcgctcggc gagggcatgg tgcaccgggt cgccggactc ccgcaggcgc
     1981 tcgacgatgc tgcgctcgtc attgatgggg tagcgctcct gcaggtgcag cagggcgttg
     2041 tattcggcgc ggtcatcgag agggtcgagc cgcaacaggg ccgccttgat ggggaacggc
     2101 aatgccgaga acctcacccg caacgagtcg tcgcggtcgc ccggtcgcag cacccggatg
     2161 tcgcggcccg caagcgccgc agcggtgccc gtactcgcgg cctcaggccg cacttcgccg
     2221 agccgcacga agtccgcgtt ggggtcgagg atcaccagcg gcagactcgt gtgcgcgagt
     2281 atctgctcca gcacgacgcc gagagcgtag gtcttgcccg acccgctctg gccgcaccag
     2341 aaggtgtggc ggttgaatcg ctgggcgttc agttctgcga tgttgcccga atcgacgacg
     2401 gtgccgatgg gcagggtggc tggcatggct cgatgctagc ggggcgttac cgcagttctg
     2461 agtcgcgccc gacgctggta tagttttcag gttggctgct cgtcgggcca gcagatgcgc
     2521 ccgtagctca gcggatagag caattgacta cggatcaata ggccgggggt tcaaatccct
     2581 ccgggcgcac cactcgagaa gaccccgtca gtttctggcg gggtcttctc gatttgttcg
     2641 tgcgggtggc actttgatca ccggggcccc gaccggcgca gtccggcgga ttgtgccatg
     2701 cgggatgcgc gcaccggcga gtgaccgcac gatctgccgg cggcaccggt tgggcgtcaa
     2761 atccctccgg gcgcacgtcg aaaaatgctg gtcagacccc tattcggtcg cccggatagg
     2821 ggtcagtacc ctacagacct acacttccgc gtgcatcagg ggctgatgcc atcagagcaa
     2881 cgtgcaatcg gtccgtcggc cgtccgcacg ttgttcacga gcgcacagcg ccagccgcgc
     2941 ttgcaccgtt gcgcgcgtcg gtcaggtcac actctccaac ccggtaccga attgctacaa
     3001 ctcagcagga tctatagggg cctgatgtga tcagcagagg gcgatttgtt cgttggggaa
     3061 gacgcgaagc ccttctaggg cgggcccccg ctgagcagcg cggtggagct tggacaatca
     3121 ggtacgcctg acggtggtga agagaagttg accggcggaa tcgcggcatt tgttgtctca
     3181 tgccgaactg ctgccgcggg ggcgcggagg tgcccgagat tgtcggagac tcagcgccca
     3241 cgctcgcacc actccgcgcg tcacacagcc ttccgcccac gacaagggct accgctccta
     3301 cgagacgggc agccagcgcc cgagcgcgta ggacgtcgtt gccgcttggt agggggcgtc
     3361 catgacgagg gcgaggaggt agcccccgag aagttgaagt cgagcactag gcgttggtct
     3421 cggccacggg cgcaggctag cgacgccgtt gatgatcaca tgcgcgcagc agttgtgact
     3481 cgaatagtca cggttgatcc aggccgctgc attaatggac cctcgtatca tgacggagtg
     3541 ggctcctcgt caaaccgcgg acccgggttt cgcattctga ttgtgctacc ggcagccgta
     3601 atgctgcttc tcgggctgag tgctgggcta gtgatcatgg gactcccgat acctgtcgcc
     3661 ctagatcggc ttccagaact tcatgcaccc ctactcgtct ttggtttcgt cggaacgctt
     3721 atcagcctcg agcgagcggt agcgctccgt gctgggtggg catacctcgc accagcgctg
     3781 atagcgggag gaatgcttct cgcgctgacg tcgctcccga tactcgtggg caaggtggtt
     3841 gtcaccgctg gacttctcgt tcacctcctt cagtattttg cgatctggcg gaggcagccg
     3901 atgacagcaa cggcggtgca ggcgctgggt gcagtggccg caatcaccgc gggtcttgcg
     3961 tggagcggcg gcgtgcgacc cgcatacctg gtgccgcttc ttgcgacctt cctgattctc
     4021 accatcgtgg gtgagcgact ggagcttgcc cgcgtcgggt cccctggtga gcgtgccgaa
     4081 ggcgcgctac tggggttcgc gttcgtactg gcggcggcca cagtgctgac attgaccgtg
     4141 cccgtgattg ccgtccccgt tgcgggcgta gcgcttctgg gcatcgtggt ctggttggcg
     4201 cgctatgaca tcgctcggat gacggtccgt cagaccggcc tgcctcgctt cgtcgcggtg
     4261 ggcctcctcg tgggattcgc ttggctcgcc gtggccggtg cagggtggat tctcggtggc
     4321 gcgcgcacgg agggccccgt ctacgacgcc gtc
//
LOCUS       DAOSOU010000186         4310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20474_length_4310_cov_5.299412, whole genome shotgun sequence.
ACCESSION   DAOSOU010000186 DAOSOU010000000
VERSION     DAOSOU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20474_length_4310_cov_5.299412"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLW15_09480"
     CDS             complement(<1..807)
                     /locus_tag="PLW15_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT34662.1"
                     /translation="MRVEVTGGVLEGTVRRGLRMWRGIPYAAAPVGELRFRAPAAVTP
                     WAGIRDASEFGPIAPQDRNGQFRVRTNAPMSEDCLTLNVIAPAEPGERMPVMVFVHGG
                     AYSVGSAREIHKQGEGLVRSGGVIFVNFNYRLGALGYLDFSSFSTRSHRFDNNLGLRD
                     QVAALEWVRDNIAAFGGDPANVTLFGESAGGNAVTTIMTVPRAGGLFARAIAQSAPPN
                     AIYPAEITAGWAREYVELLSAAVDNTSTESTSQEDAGQLLMTAPVDQLVAA"
     gene            complement(837..2915)
                     /locus_tag="PLW15_09485"
     CDS             complement(837..2915)
                     /locus_tag="PLW15_09485"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOT34663.1"
                     /translation="MSSADYSARHLSVLEGLEAVRKRPGMYIGSTDSRGLMHCLWEII
                     DNSVDEALGGHGTEIAVILHPDESVEVRDTARGIPVDIEPKTGLSGVEVVFTKLHAGG
                     KFGGGSYAASGGLHGVGASVVNALSERLDVEVDRDGKTWAMSFHRGEPGIFADSGEPT
                     PDATFTPFVSGSELRVVGKVAKGVTGTRIRYWADRQIFTKGATFQTDELLDRLRQTAF
                     LVPSLTLDLTDSRGPEPRRETFHFEGGIGEFVDHLAPDAPITDTWRMLGSGTFTETVP
                     VLQDNGHMTPTELTRECQVDVALRWGTGYDTVFKSFVNIIATPKGGTHQAGFEQGMLK
                     FLRAEVEKNARRLKVGSDKLEKDDVLAGLTAVLTVRLPEPQFEGQTKEILGTPAVRSI
                     VSSVIAKQLAEKYASSKRDDKAQSALVLDKIVSEMKSRISARAHKETQRRKNALESSS
                     LPAKLVDCRSNDVANSELFIVEGDSALGTAKLGRDSEYQALLPIRGKILNVQKASVSD
                     MLSNAECASIIQVIGAGSGRSFDLESARYGKVIIMADADVDGAHIRTLLLTLFFRYMP
                     EMIRDGRVYAAVPPLHRVVVLNPGSKPNETIYTYSEAELNAVLAGLKKSGKKYQDPIQ
                     RYKGLGEMDADQLATTTMDRAHRTLRRVNVGDAEMAAKVFELLMGNDVAPRKEFIIAG
                     HGLDRDRIDV"
     gene            3091..3321
                     /locus_tag="PLW15_09490"
     CDS             3091..3321
                     /locus_tag="PLW15_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34664.1"
                     /translation="MSQIATENTAVDELDYSLSALDRCDSCGAQAYIRVTLASGDLLF
                     CAHHGAKFKEKLFPTALNWHDESARLLESVAD"
     gene            complement(3339..4049)
                     /locus_tag="PLW15_09495"
     CDS             complement(3339..4049)
                     /locus_tag="PLW15_09495"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOT34665.1"
                     /translation="MFPELLDLNGDAQNALVLAQRARWAGWDARVHNLALGDSLEGEA
                     PQAVIVGSGAESSAPRVLEGLRALEGSLRAWSADGIPLLAVGTGWELLSQSVELSNGD
                     VLEGLGIFSGRSVGRARVSDDLVVDSDFGRLIGFENHAREYRAAAGARALGTVVYGVG
                     NSLAAEPRLEGEVVGTSIGTHLHGPVLAKNPGLADHLLGLITDGRYEATNSTAQRVDA
                     SAQAARNQIAVRLGLAAE"
     gene            complement(4069..>4310)
                     /locus_tag="PLW15_09500"
     CDS             complement(4069..>4310)
                     /locus_tag="PLW15_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HOT34666.1"
                     /translation="YFWPVDLSAMPPVTVVTGSKAHELALQLEYQGVAVAALEPDLAT
                     AIDAFFALPAPKHGIKTVIFSADSMRRTRTHLGLA"
BASE COUNT          713 a         1451 c         1419 g          727 t
ORIGIN      
        1 cgccgcgacc agctgatcga ccggggcggt catcagcagc tggccggcgt cctcctggct
       61 cgtgctctcg gtgctcgtgt tgtcgacggc ggcactcagt aactcgacgt actcgcgggc
      121 ccacccggcg gtgatctcag cgggatagat cgcgttcggc ggtgcactct gggcgatggc
      181 gcgagcgaag agtccccccg cgcggggcac ggtcatgatg gtcgtcacgg cgttcccacc
      241 ggcggactcg ccgaagagag tcacgttggc agggtcaccg ccgaacgcgg cgatgttgtc
      301 gcgcacccac tcgagcgctg cgacttggtc gcgcaggccg aggttgttgt cgaagcggtg
      361 cgagcgcgtg gagaaggagc tgaagtccag gtagccgagc gcacccagcc ggtagttgaa
      421 gttcacgaag atgacaccac ccgagcgcac cagaccctcg ccctgcttgt ggatctcgcg
      481 agcggatccc acgctgtagg cgccaccgtg tacgaacacc atcacgggca tccgctcgcc
      541 cggctcggcc ggcgcgatca cgttgagggt caggcagtcc tcggacatgg gggcattggt
      601 gcgcacccgg aactgtccgt tgcggtcctg tggtgcgatc ggcccgaact ccgaggcatc
      661 ccggatgccc gcccacggcg tcacggcggc gggtgccctg aaccgcagct cgccgacggg
      721 cgccgccgca tagggaatgc cccgccacat ccgcaggccg cgccggacgg tgccctcaag
      781 cactcccccg gtcacctcaa ctctcacgcc cgcattctag gcagtgcgag tgaccgtcag
      841 acgtcgatgc ggtcgcggtc gagtccgtgg cccgcgatga tgaactcctt gcgcggcgcg
      901 acatcgttgc ccatgagcag ctcgaacact ttcgccgcca tctccgcgtc gccgacgttg
      961 acccgcctga gggtgcggtg ggcgcgatcc atagtcgtcg tcgcgagctg gtcggcatcc
     1021 atctcaccga ggcccttgta gcgctggatc gggtcctgat acttcttgcc gctcttcttg
     1081 agcccggcca gcacggcgtt caactcggct tccgagtacg tgtagatcgt ctcgttcggc
     1141 ttcgaacccg gattgagaac gaccacccgg tgcagcggag gcaccgcagc atagacacgg
     1201 ccatcccgga tcatctcggg catgtaccgg aagaacagcg tcagcagcag cgtgcggatg
     1261 tgtgcgccat cgacatcggc gtcggccatg atgatgacct tgccgtaccg ggcggactcc
     1321 agatcgaagc tgcgacccga cccggcgccg atgacctgaa tgatcgacgc gcactcggcg
     1381 ttggagagca tgtcgctgac cgaggccttc tgcacgttga ggatcttgcc gcgaatcggc
     1441 agcaacgcct ggtactcact gtcgcgcccg agcttggcgg taccgagggc ggagtcaccc
     1501 tcgacgatga agagctcgct gttggcgacg tcgttcgagc ggcagtccac gagcttcgcc
     1561 ggcagcgagg agctctccag cgcgttcttg cgccgctggg tctccttgtg ggcgcgcgcc
     1621 gagatgcgtg acttcatctc cgacacgatc ttgtcgagca cgagggcact ctgcgccttg
     1681 tcgtcgcgct tggacgaggc gtacttctcc gccagctgct tcgcgatgac ggatgacacg
     1741 atcgagcgca cagcgggcgt gccgagtatc tccttggtct ggccttcgaa ctggggttct
     1801 ggcagccgta ccgtgagcac cgctgtcagc ccggcgagca cgtcgtcctt ctccagcttg
     1861 tcggacccga ccttcaaacg gcgcgcattc ttctcgacct cggcgcggag gaacttgagc
     1921 attccctgct cgaaaccggc ctgatgcgtt ccgcccttcg gggtcgcgat gatgttcacg
     1981 aagctcttga acaccgtgtc gtacccggtg ccccaccgca gtgccacgtc gacctggcac
     2041 tcgcgggtga gctcggtcgg ggtcatgtgg ccgttgtcct gaagcaccgg cacggtttcg
     2101 gtgaaggtgc cggacccgag catgcgccag gtgtcggtga tcggcgcatc cggagccagg
     2161 tgatcgacga actcgccgat gccgccctcg aagtggaagg tctcgcgtcg cggctcgggc
     2221 ccgcgcgagt cggtgaggtc gagggtcagc gatggcacga ggaaggccgt ctggcgcagg
     2281 cgatccagga gctcatcggt ctggaacgtc gcccccttgg tgaagatctg gcggtcggcc
     2341 cagtaccgga tgcgcgtgcc ggtgacgccc ttggcgacct tgccaacgac ccgcagctcg
     2401 ctgccggaca cgaacggcgt gaacgtggcg tccggcgtgg gctcgccgga gtcggcgaag
     2461 atgccgggct caccgcggtg gaacgacatc gcccaggtct tgccgtcgcg gtcgacctcg
     2521 acgtcgagcc gctccgagag cgcattgacc accgaagcgc cgacgccgtg cagtccaccg
     2581 gatgccgcgt acgatccgcc gccgaacttg ccgccggcgt ggagcttggt gaacacgacc
     2641 tcgacacccg agagacccgt cttgggctcg atgtcgacgg ggatgccgcg ggcggtatcg
     2701 cgcacctcga cgctctcgtc ggggtgcagg atcaccgcga tctcggtacc gtgaccgccc
     2761 agcgcctcgt cgacagagtt gtcgatgatc tcccagaggc aatgcatgag gccacgggag
     2821 tcggtggaac cgatgtacat accgggacgc ttgcgaactg cctcgagccc ctcgagcacg
     2881 gacagatgac gggccgagta gtcagcgctc gacatagatg gatctccttg ggtcgccttc
     2941 aagactaggg tcctcgaggc caccgaccct gttgccacac caccgctgta cgcgttcagc
     3001 gaaatacccg gcaaatgtcg caccgctgta acaaccgcgt gcttgtattg agtcacggga
     3061 acttcacccg ccagccgtaa ggagcaggac atgtctcaga tcgccaccga gaacaccgcc
     3121 gtcgacgaac tggactactc cctctccgcg ctcgaccgtt gcgacagctg cggagcgcag
     3181 gcctacattc gagtcaccct cgccagcggc gaccttctct tctgcgcgca ccacggcgcc
     3241 aagttcaagg agaagctgtt cccgaccgcg ctcaactggc acgacgagtc ggcgcgcctg
     3301 ctggagtccg tcgccgacta ggcgacccgc ccctcgagtt actcggcggc gagaccgagc
     3361 cgcacggcga tctggtttcg tgccgcttgg gctgaggcat ccactcgctg ggcagttgag
     3421 ttggtcgctt cgtaccgtcc atcggtgatg aggccgagca ggtggtccgc caagccggga
     3481 ttcttggcga gcaccgggcc gtgcaggtgg gttccgatgc tggtgccgac aacctcaccc
     3541 tcaagtcggg gctcggcggc gagtgagttg ccgacgccgt agacgacggt acccagcgcc
     3601 cgcgcaccgg cggcggcgcg gtactcccgg gcgtggttct cgaatccgat gaggcgcccg
     3661 aagtcgctgt cgacgacgag atcatcgctg acccgcgcgc ggcccaccga gcgaccggag
     3721 aagatgccca gcccctcaag cacgtcgccg ttcgacagct cgactgactg ggagagaagc
     3781 tcccacccgg ttcccacggc gagcagcggg atgccgtcgg cactccatgc gcgcaggctc
     3841 ccctccagcg cacgaaggcc ctccagtacc cgtggcgcgc tcgattcggc accggacccc
     3901 acgatcaccg cctgcggcgc ctcgccctca agtgagtcgc cgagcgcgag gttgtgcact
     3961 cgagcgtccc agcccgccca gcgggcacgc tgagccagaa cgagcgcgtt ctgcgcgtcg
     4021 ccgttgaggt ctaggagttc gggaaacaca gacaggatgg tgatcacatc aggccaatcc
     4081 gaggtgggtt cgcgtgcggc gcatcgaatc cgcgctgaat atgaccgtct tgattccgtg
     4141 cttcggcgcc ggcagcgcga agaaggcgtc gatcgccgtt gccaggtccg gctccagtgc
     4201 ggccaccgca acgccctggt actccagctg cagggcgagc tcgtgagcct tggaaccggt
     4261 caccacggtg acgggcggca ttgcggagag gtcgaccggc cagaagtacg
//
LOCUS       DAOSOU010000187         4292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20574_length_4292_cov_4.701440, whole genome shotgun sequence.
ACCESSION   DAOSOU010000187 DAOSOU010000000
VERSION     DAOSOU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20574_length_4292_cov_4.701440"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1739)
                     /gene="der"
                     /locus_tag="PLW15_09505"
     CDS             complement(228..1739)
                     /gene="der"
                     /locus_tag="PLW15_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT34667.1"
                     /translation="MTDNDTDDFPELDSALVERITSIDETEAAQRANALRQGLSDYDL
                     DDEDLGILDAATEDVDAITYLPALPVLAIVGRPNVGKSALVNRIIGRREAVVEDTPGV
                     TRDRVNYKGEWGQRKFTIVDTGGWEPDAKGINASVAAQAEIAIDLADAVLFVVDATVG
                     ATATDEHVVRMLRATKKPVILVANKVDDVHQEPDAAALWSLGLGEPWPVSAVHGRGVA
                     DLLDHVMTVLPEISTVAKEEVGGPRRVAILGRPNVGKSSLLNKTAGEERVVVNELAGT
                     TRDPVDEQIELGGKFWRFVDTAGIRRRVNLAQGADFYATLRTSAALEKAEVAVVVLDV
                     TEPISVQDLKIIDLVLESGRALVLAYNKWDLLDDERRIYLEKEIERDLSHVTWAPRVN
                     ISAKTGRHLEKLVPALELALASWDTRIPTGKFNALLAELTAEHPHPVRGGKQPRILFG
                     TQAASRPPTFVLFTTGFLDPQYRRFITRRLREIFGFEGSPINVNMRVREKRKR"
     gene            complement(1816..2478)
                     /gene="cmk"
                     /locus_tag="PLW15_09510"
     CDS             complement(1816..2478)
                     /gene="cmk"
                     /locus_tag="PLW15_09510"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT34668.1"
                     /translation="MTTVVAIDGPAGSGKSSVSRAAARELGFDYLDTGAAYRALAWRC
                     IEQGIDTHGPRDVIESLDSFEYSIGIDPDKFFVRVGSEDVTDDIREPRVSAIVSRVAR
                     IPEVRSYLVELFRSIIASSSRPGIIVEGRDITTVVAPDAEVRILLTATEEARMGRRSA
                     EIAGVSAEKTAQELSSRDAQDSQVVDFMNAADGVTTVDSTDLDFDQTVLAVVNLVRTQ
                     AG"
     gene            complement(2475..3551)
                     /locus_tag="PLW15_09515"
     CDS             complement(2475..3551)
                     /locus_tag="PLW15_09515"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOT34669.1"
                     /translation="MSRITGQVRIVGAGLLGASIGHALVMRGVDVILHDASRTNVSLA
                     IDYGAGRAPAEGDDPSLVIVAVPPDATADVIASELAAWPNATVTDVSSVKAGILRELE
                     SLGADLTRYVGSHPLAGRERGGAISARADLFIGRPWVITGGQGKHRRLVEDLVIELEA
                     LPVTMSAEEHDASVALVSHVPQVVSSLLARRLNGASEPAVGLAGQGLRDTTRIASSNP
                     ELWVQILGANAAPIVDILKAYRADLDAVLDALERPEAPGARKVVAEVLAGGNTGVARI
                     PGKHGQSKRFSQLVIRVDDEPGQLARLLTDVGEVGVNMEDLRLEHSPGAQFGLAEISV
                     VPEAEARLAAELEARGWTMAGASE"
     gene            complement(3548..>4292)
                     /locus_tag="PLW15_09520"
     CDS             complement(3548..>4292)
                     /locus_tag="PLW15_09520"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT34670.1"
                     /translation="GRVTVNGEIVTEPGRRVLPADLITVDGTAIQTDTTKRYVMLNKP
                     VGVVSSLADEGGRRDLREYTRDYEERLFNVGRLDAATSGLLVLTNDGELAHVLAHPSF
                     GVSKTYIAKVAGVVNAQTIAKLTRGIELDDGPIAADKARALPGSSRTETLVELTLHSG
                     RNRIVRRMMDAVGHPVIELVRRQFGPLHLGTLPVGRTRDLTKVELGQLLTIARSAEAT
                     GGRVGEAVSSSDSDTPPEAATQPAEGDAE"
BASE COUNT          633 a         1438 c         1493 g          728 t
ORIGIN      
        1 cccggaacgt tcctcgcgcc cgataacgac gctgacggca ccgaagtgga tcgcgcgcgg
       61 gagtgggggc gggcgttggg cgaggctgct ctggctgcgc gcggggttac tgcgtcggct
      121 ggttgagtag ccgcgcagcg gcgtatcgaa accgcggcgc tttggggttt cgatacgccg
      181 acttcgtcgg ctactcaacc cgctgacttt cgggggtgac ccgaaagtca gcgcttgcgc
      241 ttctcgcgca ctcgcatatt gacgttgatc ggcgagccct cgaaaccgaa gatctcgcgc
      301 agacgacgcg tgatgaagcg tcggtactgc gggtcgagga atccggtcgt gaacagcacg
      361 aacgtcggcg ggcgcgacgc ggcctgcgtg ccgaacagga tgcgcggctg cttaccgcca
      421 cgcaccgggt gcggatgctc ggcagtgagc tcggcaagca acgcgttgaa ctttccggtc
      481 ggaatgcgcg tgtcccacga cgcgagcgca agctcgagcg ccggcaccag cttctccaga
      541 tgccgcccgg tcttcgcgga gatgttcacc cgcggcgccc aggtcacgtg gctgaggtcg
      601 cgctcgatct ccttctccag gtagatgcgg cgctcgtcat ccagcaggtc ccacttgttg
      661 taggcgagca cgagcgcgcg gcccgactcg agcacgaggt cgatgatctt gaggtcctgc
      721 acgctgatcg gctcggtgac atccagcacg acgacggcca cttcggcctt ctcgagcgcg
      781 gccgaggtgc gcagggtcgc gtagaagtcg gcgccctgag cgagattgac ccgacgacgg
      841 atgcccgccg tgtcgacaaa gcgccagaac ttgccgccca gctcgatctg ctcgtcgacc
      901 gggtcacggg tggtgccggc cagttcgttg acgacgaccc gctcctcccc cgccgtcttg
      961 ttgagcaggc tcgacttgcc gacgttgggg cggccgagga tggcgacgcg gcggggtccg
     1021 ccgacctcct ccttggcgac cgtggagatc tcgggcagta ccgtcatgac gtggtcgagc
     1081 aggtcggcga cacctcggcc gtggacggct gagacgggcc agggctcgcc cagtccgagc
     1141 gaccagagcg cagcggcatc cggttcctga tgcacgtcgt ctaccttgtt ggcgaccagg
     1201 atcacgggct tcttcgtggc acgcagcatg cgcacgacgt gctcgtcggt ggcggtggct
     1261 ccgaccgtgg catccacgac gaagagcaca gcgtcggcga ggtcgatcgc gatctcggcc
     1321 tgggcggcga ccgaggcgtt gatgcccttg gcgtcgggct cccagccgcc ggtgtcgacg
     1381 atggtgaact tgcgctggcc ccactcgccc ttgtagttga cgcggtcgcg ggtgacaccg
     1441 ggggtgtcct cgacgacggc ctcgcggcgg ccgatgatgc ggttgactag cgccgacttg
     1501 cccacgttgg ggcggccgac gatcgccagc acgggaagcg cgggcaggta ggtgatcgcg
     1561 tcgacgtctt cggttgcagc atcgaggatg ccgaggtcct cgtcatcgag gtcgtagtcg
     1621 ctgagtccct gccgcagcgc gttcgcccgc tgggcggcct cggtctcgtc gatcgaggtg
     1681 atgcgctcga cgagggcgga gtcgagctcc gggaagtcgt cggtgtcgtt gtcggtcatg
     1741 ctgagggtcc tttggtttcg atgcttcgat acgccttacg gctactcagc acaagtacgc
     1801 tttacttcgg cctactcaac cggcctgcgt gcgcaccaga ttcactacag ccagaacggt
     1861 ctggtcgaag tcaagatcgg tggaatcgac ggtggtgaca ccgtctgcgg cattcatgaa
     1921 atccacgacc tgggagtcct gcgcatcgcg cgaactgagt tcctgggcgg tcttctcggc
     1981 tgagactccg gcgatttccg ctgaacgcct ccccattcta gcttcctcgg tggctgtgag
     2041 cagaatgcgc acctcggcgt cgggggcgac caccgtggtg atgtcccggc cctcgacgat
     2101 gatgcccggc cgggacgacg acgcgatgat gcttcggaac agctcgacga ggtagctgcg
     2161 cacctcggga atgcgcgcca cccgactcac gatcgcgctg acccggggtt cgcggatgtc
     2221 gtcggtgacg tcctccgagc ccacccgcac gaagaatttg tcggggtcga ttccgatcga
     2281 gtactcgaat gagtcgagcg attcgatcac gtcgcgcggg ccgtgggtgt cgatgccctg
     2341 ctcgatgcag cgccacgcga gcgcccggta ggcggcgccg gtgtcgaggt agtcgaaccc
     2401 gagctcgcgc gcggccgccc gcgacacact ggacttgccg ctgcccgccg gcccgtcgat
     2461 cgccacgaca gtcgtcattc ggatgccccc gccatcgtcc acccgcgcgc ctcgagttca
     2521 gcggcgagcc gtgcctccgc ctcgggcacc acggagatct ctgccagccc gaactgcgct
     2581 cccggcgagt gctccaaccg gaggtcctcc atgttcactc ccacctctcc cacgtcggtc
     2641 agcagccgcg ccagctggcc cggctcgtcg tcgacccgga tcacgagctg gctgaaacgc
     2701 ttcgactggc cgtgcttgcc cgggatgcgc gccaccccgg tgttgccccc ggcgagcacc
     2761 tcggcgacga ccttgcgtgc gccgggtgcc tcgggccgct cgagcgcgtc gagcacagcg
     2821 tcgagatcgg cgcggtaggc cttgaggatg tccacgatcg gcgccgcgtt cgcgcccagg
     2881 atctgcaccc agagctccgg gttgctggac gcgatgcggg tggtgtcgcg cagcccctgg
     2941 ccagcgagtc cgacggccgg ctcgctcgcg ccgttcaggc gccgggcgag caggctcgag
     3001 accacctgcg gaacgtgcga gacgagcgcg acgctcgcgt cgtgctcctc ggcgctcatc
     3061 gtcaccggca gcgcctcgag ctcgatcacg agatcctcga cgagacgccg atgcttgccc
     3121 tgtccgccgg tgatcaccca ggggcgaccg atgaacaggt cggcgcgagc ggagatcgct
     3181 cctccgcgtt cgcgcccggc gagcggatga ctgccgacgt agcgggtgag gtcggcaccc
     3241 agagactcga gctcgcgcag aatcccggcc ttgacgctcg agacgtcggt cacggtggcg
     3301 ttcggccacg cggccagctc ggacgctatg acgtcggccg tggcatccgg gggcaccgca
     3361 acgatcacga gcgacgggtc atcgccctcg gcgggcgcgc gcccggcgcc gtagtcgatg
     3421 gcgagtgaga cgttggtgcg tgaggcgtcg tgcaggatca cgtcgacccc gcgcatcacg
     3481 agcgcgtggc cgatgctcgc gccgagcagg ccggcgccca cgatgcgcac ctggccggta
     3541 atgcggctca ctcggcgtct ccttcggctg gttgagtagc cgcctccggc ggcgtatctg
     3601 agtcggagct cgatacggct tcgcctactc gaccaccggt ggcctccgcc gaccgggcga
     3661 tcgtcagcag ctggccgagt tccaccttag tgaggtcgcg ggtgcgcccg acgggcaggg
     3721 ttccgaggtg cagcggcccg aactggcggc gcacgagctc gatcaccgga tgcccgaccg
     3781 catccatcat gcgtcgcacg atgcgattgc ggcccgagtg cagcgtgagc tccacgagcg
     3841 tctcggtgcg tgacgagccg ggaagcgcgc gagccttgtc cgcggcgatc ggcccgtcgt
     3901 cgagctcgat gccgcgcgtc agcttcgcga tcgtctgggc gttgaccacg ccggcgacct
     3961 tggcgatgta ggtcttggag acgccgaacg acgggtgcgc gagcacgtgc gcgagctcgc
     4021 cgtcattggt cagtacgagc aggcccgagg tcgcggcatc cagtcgaccg acattgaaca
     4081 ggcgctcctc gtagtcgcgc gtgtactcgc gcaggtcacg gcggccgccc tcatccgcga
     4141 gcgagctcac gactccgacg ggcttgttga gcatcacgta gcgcttggtc gtgtcggtct
     4201 ggatcgcggt gccgtcgacg gtgatgaggt cggcgggcag cacgcggcgg cccggttcgg
     4261 tgacgatctc gccattgacg gtaacccggc cc
//
LOCUS       DAOSOU010000188         4272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20701_length_4272_cov_6.215082, whole genome shotgun sequence.
ACCESSION   DAOSOU010000188 DAOSOU010000000
VERSION     DAOSOU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20701_length_4272_cov_6.215082"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLW15_09525"
     CDS             complement(<1..440)
                     /locus_tag="PLW15_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HOT34671.1"
                     /translation="MTDEQESTPPSIRDSFAAAARRSGLGKVAPGENPSGSALLAAMG
                     GVRGLVESILPGLGFLVVYTITQQLLPSVLFPLGLAVIFVLVRVVTRQPWTSAVAGVV
                     GIALSAGLALLTGRAEDNFVFGFLINGAFLLALLISLAARWPLIG"
     gene            complement(437..1027)
                     /locus_tag="PLW15_09530"
     CDS             complement(437..1027)
                     /locus_tag="PLW15_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HOT34672.1"
                     /translation="MTDASDLQPVEHPKSAPADRDTAGPLDESEANAVRPYVDLGGVK
                     ILPRTDLALRLEVEESTKRVVAVGLDYAGSSLQVQPFAAPRSSGLWHEIREQIVDQIS
                     KQGGRISTRDGVFGPELIAEIPVAGGQPRLARFIGVDGPRWFLRGVISGQGVADPAAA
                     EQIEEIYRSIVVVRGTTPMPPRDLIPLHMPATGQQA"
     gene            complement(1024..1491)
                     /gene="dut"
                     /locus_tag="PLW15_09535"
     CDS             complement(1024..1491)
                     /gene="dut"
                     /locus_tag="PLW15_09535"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT34673.1"
                     /translation="MSDNVEVLISSPVVPVYAHPGDAGADLMAAEAFVLTPGARATVP
                     TGVSIALPDGYVAFVVPRSGLAAKHGITIVNSPGTVDAGYRGEIRVTVLNTDAEQPFS
                     IEVGDRIAQLIIMPVARARFVPVTTLPGSHRGESGFGSTGLRSADSTNEGVTS"
     gene            1546..2001
                     /locus_tag="PLW15_09540"
     CDS             1546..2001
                     /locus_tag="PLW15_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HOT34674.1"
                     /translation="MTIYREKLWPAPWLFISTALVIPASLLVFLPINQLAGIIVAAVL
                     YLAIIALLVMASPVIEVTDTQFIAGKARLPIAVIGGLTPHRGDDARAERGPRLDARAW
                     LLIRGWVDPVLRVQLIDEKDPTPYWIVSTRHPEAIIAAVDEVRPRTPGR"
     gene            complement(1976..2269)
                     /locus_tag="PLW15_09545"
     CDS             complement(1976..2269)
                     /locus_tag="PLW15_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HOT34675.1"
                     /translation="MATDYDAPRKTDDDSESIEALKERVPDKTSGSIDADDADNPGSF
                     ELAGADLSDLDLDVVVLPPQADEFTCVNCFLVKHRSQIDHETKLGPICQECAA"
     gene            2481..3515
                     /gene="sepH"
                     /locus_tag="PLW15_09550"
     CDS             2481..3515
                     /gene="sepH"
                     /locus_tag="PLW15_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HOT34676.1"
                     /translation="MQDLQVIGVENGALVVASDDGTRFRLPIDDVLQSKVRQSIPDTG
                     EGRKLAPKEIQAHIRSGMSAQDVASITGAPLDYIQRFEGPVIAEREFVVTSALAVAVH
                     TAADTDPLRQGKTFGTAIRERLHDLGGTNERWASWKDAASGWVVKLSFTANQIDHDAR
                     WQFDPKKHTLSPLNNEAITLSQQGEAAGTLVPRLRAVSTDEPTIDASRFDSGAFAIAD
                     SDLAGPDLPMDATAPVSLAPASISPANAPAASDDEPVHGNQTADLLEALRRRRGERES
                     APFEDDERASHPSTGGIRIVDVPTDGPGDDGPSPRRTAPQPTVRKKGRATMPSWDEIV
                     FGARPDDDLA"
     gene            complement(3512..>4272)
                     /locus_tag="PLW15_09555"
     CDS             complement(3512..>4272)
                     /locus_tag="PLW15_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT34677.1"
                     /translation="LQPTIFERASAAGFGAAAVGTERYRDSGFTRAVLRGAQYVSGSS
                     ISARMQRAAELAAQPGPPGLIYVYVPELDMAAHAHGWESREWTAALETTDSAVRDLVA
                     ALGPRAGLLVTADHGIIDVPTRSHVLIDSAPALLDGIRFVAGEPRCLQLHFEPDLSAT
                     LRSALVDQWRAAESDRSWVLTRDEFVQSGWLGAVRPDVAPRIGDIIVTARKSIAYYDS
                     RTASPHARAMIGQHGSATQPELRVPLLRFGAFAA"
BASE COUNT          753 a         1413 c         1448 g          658 t
ORIGIN      
        1 ccgatcagcg gccagcgggc agccaggctg atgagcagcg cgaggaggaa ggcgccgttg
       61 atgaggaagc cgaacacgaa gttgtcctcg gcgcgccctg tcagcagggc aagccccgca
      121 gacagagcaa tgcccacgac gccggccacg gccgaagtcc agggctgacg ggtcacaact
      181 cgcacgagca cgaagatcac ggcgagaccg agtggaaaga gcaccgaggg cagaagttgc
      241 tgggtgatcg tgtagacgac aaggaagccg aggccgggca ggatcgactc gacgaggcca
      301 cgaaccccgc ccatcgccgc gagaagggcc gacccgctcg ggttctcgcc cggcgcgacc
      361 ttgccgagac ctgagcgtcg cgccgctgcg gcgaagctgt cgcgaatcga gggcggggtc
      421 gactcctgct cgtccgtcat gcctgctgac ctgtggcggg catgtggagc gggatgaggt
      481 cacggggagg catcggggtc gtgccgcgca cgacgacgat cgagcggtag atctcctcga
      541 tctgctccgc ggccgcggga tcagccacgc cctggccgga gatcacgccg cgcaggaacc
      601 agcggggacc atcaacaccg atgaagcgcg cgaggcgggg ctggccgccg gcgacaggga
      661 tctccgcaat caactcgggg ccgaacacac cgtcgcgagt cgagatgcgt ccgccctgct
      721 tgctgatctg gtcgacgatc tgctcccgga tctcgtgcca gagcccgctc gaacggggag
      781 cggcgaatgg ctgcacctgc aacgacgaac cggcgtagtc gagaccgact gcgacgaccc
      841 gtttggtgct ctcctccacc tcgaggcgca gcgcaaggtc ggtgcgcggc aggatcttga
      901 ccccaccgag atcgacgtag ggacgaaccg cgttggcttc gctctcgtcg agcggcccgg
      961 ccgtatcccg gtcagcggga gccgacttgg gatgctcgac tggctgtaga tcagatgcgt
     1021 cggtcacgac gtgactcctt cattcgtgct gtcagcggat cgcaggccgg tcgagccgaa
     1081 gcccgactcc ccgcgatgac ttccgggcag ggtggtcacc gggacgaagc gcgcacgcgc
     1141 caccggcatg atgatgagtt gggcgatccg gtccccgacc tcgatggaga acggctgctc
     1201 cgcatcggtg ttcagcacgg tcacgcgaat ctcgccgcgg tagccggcat ccaccgttcc
     1261 cgggctgttg acgatcgtga tgccgtgctt ggccgccagc ccgcttcggg gaacgacgaa
     1321 cgcgacatag ccgtccggaa gggcgatcga caccccggtc ggcaccgtgg cgcgcgcacc
     1381 gggagtcaga acgaaggcct cggccgccat caggtcagcg ccggcatccc cgggatgagc
     1441 gtagacgggt acgacggggg atgaaatcag cacctcgacg ttgtcgctca cgtgatgagg
     1501 gtagtgcaga actctgaacg gtcagaagtc tggtcgaata gtgtcatgac gatctatcgc
     1561 gagaagctct ggcccgcccc gtggctgttc atcagcaccg ccctggtgat cccggcgagc
     1621 ctgctcgtct ttctgcctat caatcaactc gccggcatca tcgtggccgc cgtgctctat
     1681 ctggcgatca tcgcgctgct cgtcatggcg tctcccgtga tcgaggttac ggacacccaa
     1741 ttcatcgctg gtaaggcccg tctgccgatc gccgtgatcg gcggcttgac cccccaccga
     1801 ggggatgacg cacgcgcaga gcgtggacca cgtctcgatg cccgagcctg gctgctcatc
     1861 cgcgggtggg tcgaccccgt cctgagggtg caactcatcg acgagaagga cccgacgccc
     1921 tactggatcg tgtccacccg acacccggag gcgatcatcg ccgcggtcga tgaggtcagg
     1981 ccgcgcactc ctggcagata ggcccgagct tcgtctcgtg atcgatctgc gagcggtgct
     2041 tcaccaggaa gcagttgacg caggtgaact cgtcggcctg aggaggcagt acaaccacgt
     2101 cgaggtcgag gtcggagaga tccgcccccg ccaattcgaa acttcccggg ttgtcagcgt
     2161 catccgcgtc gattgaaccc gaggtcttgt ccgggacgcg ctccttgagg gcctcgatcg
     2221 actcggagtc gtcgtcggtc ttacggggtg cgtcgtagtc ggttgccatg cccatccact
     2281 tctgtatgcg tcgtttctca gtgctgcgta acgaaatcgg cggggacagt gtgcatcatc
     2341 ccaaggggaa tagcaaaccg ctgaacgacc gtcgcgttga cgctagacga acaacttgcg
     2401 gcacgcctgt tctattcccg ggaaacgctg gtcagtcgtg tcatccttgt ggcaatcgtg
     2461 acggctggag ggcattcagc atgcaggacc tgcaggtaat cggagtggag aacggcgcac
     2521 tcgtcgtcgc ctccgacgac ggtacgcgct tccgtctccc catcgatgat gtgctccagt
     2581 cgaaggttcg acagagcatt cccgacaccg gggaggggcg caagctcgcg cccaaggaga
     2641 tccaggccca cattcgctcg ggcatgtctg cgcaggatgt cgcctcgatc accggcgccc
     2701 cgctcgacta cattcaacgt ttcgaggggc cggtgatcgc cgagcgcgag ttcgtggtca
     2761 cctccgcgct tgcggtcgcg gtgcacacgg cggcggacac cgacccgcta cggcagggca
     2821 agaccttcgg aacggccatc cgcgagcggc tgcacgacct gggcggaacc aatgagcgct
     2881 gggccagctg gaaggatgcc gcgagcggct gggtcgtcaa gctctcgttc accgccaacc
     2941 agatcgatca cgatgcccga tggcagttcg atcccaagaa gcacacgctg agccctctca
     3001 acaacgaggc aatcaccctc tcccagcagg gcgaggcggc gggcaccctg gttcctcggc
     3061 tgcgggcagt gtcgaccgac gagccgacta tcgatgcctc acggttcgac agcggagcct
     3121 tcgcgattgc cgactccgac ctggctggcc cagatcttcc catggatgcg acggcgcccg
     3181 tatcgctcgc tccggcgtcg atcagcccgg cgaacgcccc cgcggccagc gacgacgagc
     3241 cggtgcacgg caatcagacc gccgatctgc tcgaggccct gcgccgccgg cgtggggagc
     3301 gggagtcggc accgttcgag gacgacgagc gcgcctcgca tccgtcgacc ggcggcattc
     3361 gcatagtgga cgtgccgacg gacggccccg gcgatgatgg gccatcacct cgccgcacgg
     3421 cgccgcagcc cacggttcgc aagaagggcc gcgcgacgat gccgagctgg gacgagatcg
     3481 tcttcggcgc gcgccccgac gacgacctgg cctaggccgc gaaagccccg aaacgcagca
     3541 gcgggacgcg cagctcgggc tgcgttgcgg agccgtgctg accgatcatc gctcgggcgt
     3601 gcgggctcgc ggtgcgggag tcgtagtagg cgatcgactt gcgggcggtg acgatgatgt
     3661 ctccgatcct tggcgcgacg tcgggtcgca ccgctcccag ccagccggac tgcacgaact
     3721 cgtcgcgagt cagcacccag gaccggtcgg actcggccgc gcgccactgg tcgaccaggg
     3781 ccgaccgcag cgtcgcagac aggtcgggtt cgaagtggag ctgcaggcac cgcggctccc
     3841 ccgccacgaa ccgaatgccg tcgagcaggg ccggggcaga gtcgatgagc acatgagatc
     3901 gggtgggaac gtcgatgatc ccatgatcgg cggtgacgag cagacccgca cgtgggccca
     3961 gcgccgccac caagtcgcgc acggccgagt cggtcgtctc caaggcggcc gtccactcgc
     4021 gtgactccca gccgtgggca tgggcggcca tgtcgagctc gggcacgtag acgtagatga
     4081 ggccgggcgg acccggctgt gccgccagtt cggcggcccg ctgcatccgt gcggagatgg
     4141 atgacccact cacgtactgc gcaccgcgca gcactgcgcg cgtgaagccg gagtcgcggt
     4201 acctctcggt tccgacggcg gccgcaccga agcccgccgc ggaagcccgc tcgaagatgg
     4261 tgggttggag ct
//
LOCUS       DAOSOU010000189         4259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20800_length_4259_cov_6.257136, whole genome shotgun sequence.
ACCESSION   DAOSOU010000189 DAOSOU010000000
VERSION     DAOSOU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20800_length_4259_cov_6.257136"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..393)
                     /gene="ssrA"
                     /locus_tag="PLW15_09560"
     tmRNA           complement(17..393)
                     /gene="ssrA"
                     /locus_tag="PLW15_09560"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            568..1251
                     /locus_tag="PLW15_09565"
     CDS             568..1251
                     /locus_tag="PLW15_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOT34678.1"
                     /translation="MADTIITVQGEYSAWYPAERATVRASVQVDGPKRDAVFTSAVTA
                     SDTLRVTIEALFDKVKGPVTWWTSDSVRVWSERPWNNEGKQLPLVYHSAVEFSAKFSD
                     FDALSRWVESAAAVEHVSVGSISWDLTDATRTSATTEVRSRAVKDAVAKASVFAQSIG
                     LGSVKAIALADPGMLGDPSSGGGAAPAPMFRGAMKAQAFDGGSSPVLALKPEEIAVAS
                     VVDARFVAS"
     gene            complement(1254..1883)
                     /locus_tag="PLW15_09570"
     CDS             complement(1254..1883)
                     /locus_tag="PLW15_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34679.1"
                     /translation="MTRQRVVLEVVTAVLLGLVSVGTTFSAYQATVLNQQAADLDSIS
                     QQQRDRNLTKFLSSQLTFRDDSRRVSAAFALQSELILRPENAEQVAAERDALAAAGSP
                     ALRDTWPAWAAAGYGIESFPLADPDYTVALFAESQSLQYASFVADGMVDRVTARAQTL
                     TGAAVIFALALFVLGVAGVLTAPRAVISLAAGGAALFVVALVITLVASG"
     gene            complement(1880..2530)
                     /locus_tag="PLW15_09575"
     CDS             complement(1880..2530)
                     /locus_tag="PLW15_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34680.1"
                     /translation="MPEERPSRALDVITAIMLGLVSVVTALGAWQAAAWDGRAQEFGR
                     DAGDARDVSVNQSVQADYAARTDLEASLAARAIADRREGQIDPLELALIDNEIQATLS
                     STTPGFSDAWYLWQSGGFDPTSAPVDDPDYLAGRDGFYQSYGVAGFVLDDLSHQLQTR
                     SSVLAQAALVQALALFLIGIAGVNRVGAVRVAILALGAVVFLVGIVVALSSFGVGA"
     gene            2579..3094
                     /locus_tag="PLW15_09580"
     CDS             2579..3094
                     /locus_tag="PLW15_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HOT34681.1"
                     /translation="MTVDSAPPTKRGLPRWAIVLIAVGGAIAVTVIGLVIAVIVSVFS
                     GPAWGPTEAAALQPGSCLAENGADLAVYTVVDCDQPHPQQVFAEIDLSRNTAQYTTQT
                     ALTTYAGEICARFLEYGLFVTEAADDTVTTGPLAVPTPEALAAGSSRALCSLSGADGS
                     SLTGDLYRPMP"
     gene            complement(3108..3584)
                     /gene="smpB"
                     /locus_tag="PLW15_09585"
     CDS             complement(3108..3584)
                     /gene="smpB"
                     /locus_tag="PLW15_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT34682.1"
                     /translation="MPRERGQKVVASNRKARHDYTIEDTFEAGLVLTGTEVKSLRAGR
                     ASLVDGYGFIEGGEAWLDAVHIPEFSQGSWNNHPPRRKRKMLLHKAQILKIHNKVKEG
                     GYTLVPLSIYFSDGNAKVELAIAKGKREYDKRQTLRERQDKREADRAMSARKNLNE"
     gene            complement(3641..>4259)
                     /gene="ftsX"
                     /locus_tag="PLW15_09590"
     CDS             complement(3641..>4259)
                     /gene="ftsX"
                     /locus_tag="PLW15_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT34683.1"
                     /translation="FEYENHEQAYKNFQAQLSDNPIASFVTPETLNESFRVNLIDPTE
                     ADILIESLSSMPGVQAVEDQRGYLDRIFAVLNAASYTAIGVAALMLIAAVLLIATTIR
                     LSAFSRRRELGIMRLVGASNRFIQTPFILEGVFAALIGSVLAGLAIVGIVQFFVQGYL
                     TTALQSTTTLVGLGDALVVVPILVVVGAALAAASAGVAISRYLKV"
BASE COUNT          728 a         1422 c         1392 g          717 t
ORIGIN      
        1 cgcgatagcg acctgatggt ggagatgggg ggaattgaac ccccgtccac tgctgtaatt
       61 ccacgccttc tacgggtgta gctcgtgaag tcgttctact cggccctgac cattgccacg
      121 agcatcttgg tcaacaggcc cagcctcagt tgaagtcccg cgtgtccctg agactcaagc
      181 acgcagcaat ctctctagat aacgccagga accgggtaga gagcatcccc gggctgacgg
      241 acctttaagg gctagttgct gctaggcagc aagagcgagg tcagtgcgct taacattggc
      301 acttattttt tgcagaggtc gtttacgaga taaccctgca tcctcgaccc gcttctcgta
      361 gttttgcagg caatgtcgaa accgatcatc cccatgcttc gcttcgtccg accgtgaggc
      421 cgaagttctg cgggtcgcta tcgcgctgtt gagttgtcaa acccgcccgg tttcccgagc
      481 agccttccat tgtacgccgc caagatactc catgccaccg gcggcccatg gcaggatcgg
      541 aagccagact tcgacgacgg gagcagcatg gcagacacga tcatcacggt gcagggcgag
      601 tactcggcgt ggtacccggc cgaacgggcc accgtgcggg cgagcgtgca agtcgacgga
      661 ccgaagcggg acgcggtctt caccagcgcc gtgaccgcct ctgacaccct gcgcgtgacc
      721 atcgaggccc tgttcgacaa ggtgaagggt cccgtcacgt ggtggacctc cgacagcgtg
      781 cgggtctggt ctgagcgccc ctggaacaac gagggcaagc agctgcccct cgtgtaccac
      841 tccgccgtgg agttctcggc gaagttcagc gacttcgacg cgctgtcacg gtgggtcgag
      901 agcgccgcgg ccgtcgagca cgtcagcgtc gggtcgatca gctgggacct gaccgacgcc
      961 acccgcacgt cagccaccac cgaagtgcgc tcccgagctg tgaaagacgc cgtcgcgaag
     1021 gcgagcgtgt tcgcccagtc gatcggcctc ggtagcgtga aggcgatcgc cctcgccgac
     1081 cccgggatgc tcggggatcc gtcgagcgga ggtggcgcgg ctcccgcacc gatgttccgc
     1141 ggtgcgatga aggcacaggc gttcgacggc gggagctcgc cggtgcttgc actcaagcct
     1201 gaggagattg ccgtggcatc cgtcgtggat gcccggttcg tggcgagctg agctcagccc
     1261 gacgcgacca gggtgatcac gagggcgacc acgaacagcg ccgctccgcc cgccgcgagc
     1321 gagatgaccg cacggggcgc ggtgagcacc cccgcgacgc ccagcacgaa cagggcgagc
     1381 gcgaagatca ccgctgcccc ggtcagggtc tgggcgcgag ctgtgacacg atcgaccatg
     1441 ccgtcggcga cgaatgacgc gtattgcagc gactgggact cggcgaacaa ggcgaccgtg
     1501 tagtcggggt cggcgagcgg gaagctctct atgccgtaac cggcagccgc ccaagccggc
     1561 cacgtgtcgc gcagtgctgg gctgcctgct gcggcgagcg catcccgctc ggcggcgacc
     1621 tgttcggcgt tctcgggtcg caggatcagt tcgctctgca gcgcgaatgc ggcgctcaca
     1681 cgtcggctgt cgtcccggaa ggtgagttga ctcgacagga acttggtgag attcctgtcg
     1741 cgttgctgct gggagatcga gtcgaggtcg gccgcctgct ggttcagcac ggtcgcctgg
     1801 taagcactga aggtggtgcc gacggagaca agccccagaa gcacggctgt gaccacctcg
     1861 agcaccactc gttgccgggt catgcgccca ctccgaagga gctcagggcg acgacaatcc
     1921 ccacgaggaa caccacagcc ccgagcgcga ggatggcgac ccgcacggcg cccacccggt
     1981 tgacgccggc gatgccgatg aggaagagcg cgagcgcctg cacgagcgcg gcctgcgcga
     2041 gcacgctcga ccgtgtctgc agctggtgcg agaggtcgtc cagcacgaag cctgccaccc
     2101 cgtaagactg gtagaaaccg tcacgccccg cgagatagtc cgggtcgtcc accggtgcgg
     2161 atgtcgggtc gaaaccgccc gactgccaga ggtaccaggc atctgagaat cccggagtcg
     2221 tgctgctgag cgtcgcttgg atctcgttgt cgatgagcgc cagctcgagc ggatcgatct
     2281 ggccctcgcg tcgatcggcg atcgcccgcg cggccaagga tgcctcgagg tcggttcgcg
     2341 cggcgtagtc ggcctgcacc gactggttga cgctgacatc ccgcgcgtcg cctgcgtcac
     2401 gaccgaattc ctgggctcgg ccgtcccagg cagcggcctg ccacgccccc agcgccgtga
     2461 ccaccgagac cagacccagc atgatcgcgg tgatcacgtc cagagcgcgg gacgggcgtt
     2521 cttctggcac gagtgacatt caatcaactg cgccccgtct cgcgctacgg tattcgctgt
     2581 gaccgttgat tccgccccgc ccacgaagcg cggcctcccc cgctgggcga tcgtgctcat
     2641 cgccgtcgga ggcgccatcg ccgtgaccgt catcggcctg gtgatcgccg tcatcgtgag
     2701 cgtcttctct ggcccggcct ggggacccac cgaggctgcg gcgctgcagc ccggctcctg
     2761 cctcgcggag aacggcgccg acctcgccgt ctacaccgtg gtcgactgcg atcaaccgca
     2821 cccccagcag gtcttcgccg agatcgacct ctcccgcaac accgcccagt acacgactca
     2881 gaccgccctc accacctacg ccggtgagat ctgcgcacgg ttcctggagt acgggctgtt
     2941 cgtgaccgag gccgcagacg ataccgtcac gaccgggccg ctggccgttc ccacacccga
     3001 agcactggcg gccggtagct cacgggcgct gtgcagcctg tcgggagccg acgggtccag
     3061 cctcacgggc gacctgtacc ggccgatgcc gtgacgaaga tccccggcta ctcgttgagg
     3121 ttcttgcggg ccgacatcgc gcggtcggcc tcgcgcttgt cctgacgctc gcgcagtgtc
     3181 tgacgcttgt cgtactcgcg cttgcccttc gcgatagcga gctccacctt cgcgttgccg
     3241 tcgctgaagt agatcgacaa cggcacgagg gtgtagccac cctccttgac cttgttgtgg
     3301 atcttgagga tctgcgcctt gtgcagcagc atcttgcgct tgcgacgggg cgggtggttg
     3361 ttccacgacc cctggctgaa ctcggggatg tgcacagcgt cgagccaggc ctcaccgccc
     3421 tcgatgaagc cgtagccgtc gaccaggctc gcgcgacccg cgcgcaacga cttgacctcg
     3481 gttcccgtca agaccagccc ggcctcgaag gtgtcctcga tcgtgtagtc atggcgcgcc
     3541 ttgcggttgc tggcaacaac cttctggccg cgttccctgg gcatcgtctc tctccgtcag
     3601 taggtgcccg tccgggcagc ccaccatcct aacgcgggag ctagaccttg aggtacctgc
     3661 tgatcgccac gccagcggat gccgcggcaa gagcagcgcc gacgaccacg agtatcggca
     3721 ccacaaccag cgcatcgccg agtcccacga gggtggtcgt ggactgcagc gcggtggtga
     3781 ggtagccctg cacgaagaac tgcacgatgc cgacgatcgc gagccccgcc agcaccgagc
     3841 cgatgagcgc cgcgaagacg ccctcgagta tgaagggggt ctggatgaac cggttcgagg
     3901 caccgacgag ccgcatgatt cccagctccc gccgtcggga gaaggcactg agccggatgg
     3961 tcgtggcgat cagcagcacc gccgcgatca gcatgagcgc agcgacgccg atggccgtgt
     4021 agctggccgc gttgagcacg gcgaagatgc gatcgaggta gccgcgctgg tcttcgaccg
     4081 cctgcacacc gggcatgctc gacagcgact cgatgaggat gtcggcctcg gtcggatcga
     4141 tgaggttcac ccggaagctc tcgttgagcg tctcgggggt gacgaagctc gcgatcgggt
     4201 tgtcggagag ctgcgcctgg aagttcttgt aggcctgctc gtgattctcg tactcgaac
//
LOCUS       DAOSOU010000190         4237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20952_length_4237_cov_5.939264, whole genome shotgun sequence.
ACCESSION   DAOSOU010000190 DAOSOU010000000
VERSION     DAOSOU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20952_length_4237_cov_5.939264"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..3043)
                     /locus_tag="PLW15_09595"
     CDS             complement(149..3043)
                     /locus_tag="PLW15_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOT34684.1"
                     /translation="MSSTAASGTSNAPANRTRVTLTITAIVIAVVVIAFFVVANLYTD
                     VLWFDQLGYLNVLTTQWIAGAIMFFVGFFGMAVPVWASIELAFRARPVYAKLNSQLDR
                     YQQVIEPLRRLAMFGIPVVLGVFAGISTATRWPLVWQWLNKTEFGETDPQFGLDLSFY
                     FYDLPLWQGVVGFASAVVLIAGLAALATSYLYGAIRFSGREVRISRSARIQLAITAAI
                     YLALQAVSLWLDQYATLYSTSQGFLNTGAGYTEANATIPGRAILAGIAALVAVLFIVT
                     AIIGRWRLPIIGTALLLVSGLVVGSIYPWIVQRFQVEPSARTYEAPYIERNIAATRAA
                     YGVDGVQEVPYSAKTDAEPGALRLDAETTANIRILDPAIAPQAFAQLEQFKQYYQFAN
                     HLDVDRYTIEGKTQDTVIAVRELNQAGLGDSRSWYNDTVVYTHGYGVVAAYGNQRATD
                     GQPVFLESGIPSTGELGDFEPRVYFGEESPEYSIVGGAPGAKDIELDYPSGGEDSNQN
                     ATTTFSGDGGPKLDNIFTKLIYAIKFQSEQIFLSDAVTNDSQILYDRNPQDRVAKVAP
                     YLTLDADTYPAIVDGRIVWIVDGYTTGANYPYSKVEQLSSAIADTYTPAPAFAVDDIN
                     YIRNSVKATVDAYDGSVKLYAWDDKDPVLKTWSKVFPTTLLPMSDMSEQLLDHVRYPS
                     DLFKVQRAILGSYHVTDAGSFYSSDDQWITPNDPVAATGNQTLQPPYYLTMQVPGTDQ
                     PAFTLYSTYIPNASGDSSRNVLTGYLAVDSDAGPNYGKLTLLTLPKQTTVPGPGQVET
                     NFKTDTAVANQLALLQRGETEVKLGNLLTLPVGGGLLYVQPVYVQATSGTIYPLLRKV
                     LVSFGDQIAFEDSLDQALDTLFGGDSGASAGDGTVPPTPTDPTTPTDPTTPTTPTTPT
                     DNAALTQALADYQAALADRQQAYADGDLVAAAEADKLMQEAVARAIAALG"
     gene            complement(3111..4229)
                     /locus_tag="PLW15_09600"
     CDS             complement(3111..4229)
                     /locus_tag="PLW15_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT34685.1"
                     /translation="MALTLFTDLDPDPPARRRASGWVGWSLLLVALVGAVIVALLPAP
                     YVIEQPGPVYDVLGDVTVNGDQVPMIQIPMEETFPTEGSLDMLTVRVLGSPDSLPNWL
                     DVATAYLDPSRAIVPVEEIYPPGVTSEQSTEQGKVDMANSQKEAIAAALTYLGNEVPS
                     TLSVAEVQAGGPSDGLLEVGDVIETVNGVTFPDVTGLRAAIADNGVSQPAEVTVLRAG
                     VSTTLRINPELSAGDNPVPILGIVVASDYDFPFTVTIQLENVGGPSAGMMFALGIVDK
                     LTPGSLNGGESVAGTGTISSTGEVGPIGGIRQKMWGAKNAGADWFLAPSSNCDEVTGH
                     IPGGLTVLSVTTLDDAMTALAAIRGDGSTAGLPTCPTK"
BASE COUNT          697 a         1331 c         1483 g          726 t
ORIGIN      
        1 tgcgacctct gggttatgag cccagcgagc taccgagctg ctccaccccg cgtcgtgctt
       61 ccagagtaac acaggcgcgg ggtggggccg acggggtcgc ggtttcgata cgcggcccca
      121 gcggggccgc tactcaacca gcgcggtact agcccagggc agcgatcgct cgggccaccg
      181 cctcctgcat gagcttgtcg gcctcggccg ccgcgacgag gtcgccgtcg gcgtacgcct
      241 gctggcgatc ggccagcgcg gcctggtagt cggccagggc ctgcgtcagt gcggcgttgt
      301 cggtcggcgt cgtcggagtg gtcggcgtcg tcgggtccgt gggtgtggtg ggatcggtcg
      361 gcgtgggcgg cacggtgccg tcaccggcag atgcacccga gtcgccgccg aacagcgtat
      421 ccagagcctg atcgagggag tcctcgaagg cgatctggtc accgaacgag acgagcacct
      481 tgcgcagcag cgggtagatc gtgcccgatg tcgcctgcac gtagacaggc tgcacgtaga
      541 gcagtccccc acccacgggc aatgtcagca ggttgccgag cttgacctcg gtctcacccc
      601 gctgcagcaa cgcgagctgg ttggccacgg cggtgtccgt cttgaagttg gtctccacct
      661 ggccggggcc gggcaccgtg gtctgcttgg gcagcgtcag cagcgtgagc ttgccgtagt
      721 tgggtccggc gtcagagtcg actgcgaggt atccggtgag cacgttgcgg ctggagtcac
      781 cactcgcgtt cgggatgtac gtcgagtaca gcgtgaacgc cggctggtcg gtgcccggca
      841 cctgcatggt gaggtagtag ggcggctgca gcgtctggtt gcctgtcgcg gccaccgggt
      901 cgttgggcgt gatccactgg tcgtcgctcg agtagaacga gcccgcgtcg gtcacgtggt
      961 acgagccgag gatcgcccgc tgcaccttga acaggtctga cgggtagcgc acgtggtcga
     1021 gcagctgctc gctcatgtcg ctcatcggca gcagcgtggt cgggaagacc ttcgaccagg
     1081 tcttcagcac cgggtccttg tcgtcccacg cgtacagctt cacgctgccg tcgtaggcgt
     1141 cgacggtcgc cttcaccgag ttgcggatgt agttgatgtc gtcgacggcg aacgccggtg
     1201 ccggcgtgta cgtgtcggcg atagcactcg agagctgctc caccttggag tacgggtagt
     1261 tcgcaccggt cgtgtagcca tcgacgatcc agacgatgcg cccgtcgacg atcgccgggt
     1321 aggtgtcggc gtccagcgtc aggtagggcg cgaccttggc cacgcggtcc tgcgggttgc
     1381 ggtcgtagag aatctgcgaa tcgttggtca cggcatccga caggaagatc tgctcggact
     1441 ggaacttgat cgcgtagatc agcttcgtga agatgttgtc gagcttcggc ccaccgtcgc
     1501 cggagaaggt cgtcgtcgcg ttctggttgc tgtcctcgcc accggagggg tagtcgagtt
     1561 cgatgtcctt ggcaccgggc gccccaccga cgatcgagta ctcgggcgac tcctcaccga
     1621 agtagacgcg gggctcgaag tcacccagtt cgccggtgct cgggatgccc gactcgagga
     1681 acacgggctg gccgtcggtc gcccgctggt tgccgtacgc cgcgaccacg ccgtagccgt
     1741 gggtgtagac gacggtgtcg ttgtaccagg agcgggagtc gcccaggccg gcctggttga
     1801 gctcgcgcac cgcgatcacg gtgtcctggg tcttgccctc gatcgtgtag cggtccacgt
     1861 ccaggtggtt ggcgaactgg tagtactgct tgaactgctc gagctgcgcg aaggcctgcg
     1921 gggcgatcgc ggggtcgagg atgcggatgt tcgccgtcgt ctcggcatcg aggcgcagcg
     1981 cgcccggctc ggcgtcggtc ttcgcgctgt agggcacctc ctgcaccccg tcgaccccat
     2041 aggccgcccg ggttgccgcg atattgcgtt cgatgtacgg cgcttcgtag gtgcgagcgc
     2101 tcggctcgac ctggaagcgc tgcacgatcc acgggtagat gctgccgacg acgagaccgc
     2161 tcaccagcag cagcgcggtg ccgatgatcg gcaggcgcca gcgaccgatg atcgccgtga
     2221 cgatgaagag cacggcgacg agggcagcga tgcccgcgag gatcgcacga ccggggatcg
     2281 tcgcattcgc ttcggtgtaa cccgcacccg tgttgaggaa gccctggctg gtgctgtaga
     2341 gggtcgcgta ctggtcgagc cagaggctca ccgcctgaag cgcgaggtag atggcggcgg
     2401 tgatggcgag ctggatgcgc gccgaacgcg agatgcgcac ctcgcgaccg ctgaaccgga
     2461 tcgccccgta gaggtagctg gtggcaagcg ccgcgagacc cgcgatgaga acgaccgcag
     2521 acgcgaagcc cacgacaccc tgccacagcg gcaggtcgta gaagtagaac gacaggtcga
     2581 gaccgaactg cgggtcggtc tcaccgaact cggtcttgtt gagccactgc cacacgagcg
     2641 gccagcgtgt cgcggtggag attcccgcga agactccgag caccaccggg atgccgaaca
     2701 tcgcgagacg acgcagcggc tcgatgacct gctggtagcg atccagctgc gagttgagct
     2761 tcgcatagac ggggcgcgcg cggaaggcga gctcgatgct cgcccacacg ggcacggcca
     2821 tgccgaagaa cccgacgaag aacatgatcg cgccggcgat ccattgcgtg gtcagcacgt
     2881 tgaggtaccc gagctggtcg aaccacagca catcggtgta gaggttcgct acgacgaaga
     2941 aggcgatgac gacaaccgcg atcacgatgg ccgtgatcgt gagggtgacc ctcgtccggt
     3001 tggcgggcgc gttggacgtt ccggacgctg cggtgcttga cacgaggttc tccatgctgt
     3061 tggggactgc gcccatctta agggccgaac ctgctaagtc gcccgaaagc tcacttcgtg
     3121 gggcaggtcg gcaatcccgc ggttgacccg tctccgcgga tcgcggcgag cgccgtcatg
     3181 gcgtcgtcca gggtggtgac cgacagcacg gtgagtccgc ccgggatgtg ccctgtcact
     3241 tcatcgcagt tcgatgacgg cgccaggaac cagtcagcac cagcattctt ggcgccccac
     3301 atcttctgcc ggatgccgcc aatcggcccg acctcgcccg tcgagctgat cgtgccggtg
     3361 ccagcgaccg actccccacc gttgagcgag cccggcgtga gcttgtccac gatgccgagg
     3421 gcgaacatca tgcccgcgct cggccctccg acgttctcca gctggatggt gacggtgaac
     3481 gggaagtcgt aatcgctcgc caccacgatg cccaggatag gtaccgggtt gtcgccggcg
     3541 ctgagctccg gattgatgcg tagggtcgtc gacactccgg cgcgcaacac ggtcacttcc
     3601 gccggctggc tcacgccgtt gtcggcgatc gcggcacgga ggcccgtgac atccgggaac
     3661 gtcaccccgt tgaccgtctc gatgacgtca ccgacctcga gaagcccgtc ggatgggccg
     3721 cccgcctgca cctcggcgac actgagcgtg ctcggaacct cgttgcccaa gtaggtgagg
     3781 gcggcggcga tggcctcctt ctgggagttg gccatgtcga ccttgccctg ctcggtggac
     3841 tgctcagagg tgacgccggg agggtagatc tcctcgaccg gcacaatggc ccggctcggg
     3901 tcgaggtagg cggtggccac atcgagccag ttcggcaggc tgtccgggct tccgagaacc
     3961 cgcacggtga gcatgtcgag ggagccctca gtggggaagg tctcctccat cgggatctgg
     4021 atcatcggaa cctgatcacc gttgaccgtg acatcgccga gcacgtcgta gaccggaccg
     4081 ggctgctcga tcacataggg cgcgggaagc agcgcgacaa tgacggcacc gacgagcgca
     4141 acaagcagca gggaccaccc gacccatcca ctggcacgac ggcgtgctgg cggatcaggg
     4201 tcgagatcgg tgaacagggt cagagcgatc ctccggg
//
LOCUS       DAOSOU010000191         4234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_20978_length_4234_cov_4.924623, whole genome shotgun sequence.
ACCESSION   DAOSOU010000191 DAOSOU010000000
VERSION     DAOSOU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20978_length_4234_cov_4.924623"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /gene="mtrB"
                     /locus_tag="PLW15_09605"
     CDS             <1..1069
                     /gene="mtrB"
                     /locus_tag="PLW15_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HOT34686.1"
                     /translation="GARYELYLVYNIADVAQTLGFVQQTLVFGGFALVLLIGAVTYIV
                     VRLVVGPVQLAAETSEKLAAGQLEVRIPVKGDDVIATLARSFNGMAASLQNQITRLAA
                     LSQVQQRFVSDVSHELRTPLTTIRLAGDVLYDQRESFPPATARTAELLHTQVERFELL
                     LADLLEMSRYDAGAVDMETEPTNLVRLVEDAVHTVASLAESKGSELRVVAPGGYFEAE
                     VDARRIRRIISNLLGNAIDHGEGRPIVIWVDSDKEAVAIAVRDYGVGMTAAAMERVFD
                     RFWRADPSRQRTTGGTGLGLAISLEDAALHGGWLQVWSAPGEGSCFRLTLPRVRGAVL
                     KSSPLELPPPEETATLGVLDA"
     gene            1062..2711
                     /locus_tag="PLW15_09610"
     CDS             1062..2711
                     /locus_tag="PLW15_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HOT34687.1"
                     /translation="MRRALILGFVVCLALTGCVGIPTSGGVETGPVIDEQLDPDFVID
                     PSGPRAGSAPEDILTDFMLALRGPQGNYEIAREFLTKDFATKWSPDASATIRTGIPAT
                     TPGEAPNTLDYTITTRAYVDADGRYFEPGPATQVLTYSFVQENGEWRISGGPDGIVLS
                     QSSFSVVFTERALYFFDPTYTYLIPDVRWFASRATLPGWAVRELLDGPAQWLQQGVVL
                     SAFPISTTLDTASIDSGVATVELSKEALAASATDRDRMRQQLAATLDVANVVMTVSGI
                     DISAPATGPQATKDPSVEPATLVGTDTEFGFDTGAGVAPLEGLSATVIAGGATAATLS
                     NDEQYVAFLGPDGVSVAQRGNDLGDPVDARPGLVAPSIDPFRFVWSAQAESAATISTF
                     EIDGTEHALTSSAPADSAVVSLDVSRDGARLLLYLSTPLGPRLAVAGIIRDQKNVPTA
                     LGQLEYLPVASNAPIDATWVDARTVATVSRGGEVAPVTLYEIGGPSATFGQVPDASTI
                     VGGNGGADGLRVLRTSGEVLRPQGSSWVTTGLSARVLATKQ"
     gene            2768..3523
                     /locus_tag="PLW15_09615"
     CDS             2768..3523
                     /locus_tag="PLW15_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT34688.1"
                     /translation="MPLRDALLDALLSALPPALRDALLDAWSVLFPVDCAGCDSADRS
                     LCDECRAALQPDVTVRSVGGPGPDGLGVHSGARYEGRVRRVILAFKEQGRTDAAAALS
                     RPLAAAVAAALSTAPAAELLLVPTSRAAYRRRGYDPVRLLASRAGLRVARELRQVRAT
                     GAQKALGVEQRAANRDGAFRARRDLNGRSFIVVDDVVTSGATILEVARAVRAAGGDVL
                     GAATLAFTPRLLPIRDIAEGQEYGNDKEAHKTA"
     gene            3564..>4234
                     /gene="raiA"
                     /locus_tag="PLW15_09620"
     CDS             3564..>4234
                     /gene="raiA"
                     /locus_tag="PLW15_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT34689.1"
                     /translation="MDITITGRNVGVTDRFREYATEKAGKIDHLADKAIAFEVKVARH
                     HETRGSSGDDRVELTLIGPGPLVRAESSGSDKYVAFDLAMAKLVERMRQSKDRRKVHR
                     GNHRPVSLREASADGFSIVDITPASVEVLERVATGEIPVQAVDAAEEEYSPVVIREKV
                     FPSTHMTVSDAVDHMELVGHDFFLFIDAETDRPSVVYRRKGWDYGVIGLEEQQEAPAK
                     RSRRA"
BASE COUNT          627 a         1481 c         1470 g          656 t
ORIGIN      
        1 cggtgctcgc tacgagctgt acctcgtcta caacatcgcc gacgttgcgc agaccctcgg
       61 gttcgtgcag cagacgctgg tcttcggcgg gttcgcgctc gtgctcctca tcggtgccgt
      121 cacctacatc gtggtgcggc tcgtcgtcgg accggtgcag ctcgccgccg agaccagcga
      181 gaagctggcg gcgggccaac tcgaggtgcg catccccgtg aagggcgacg acgtcatcgc
      241 caccctcgcc cgctcgttca acggcatggc tgcgagcctg cagaaccaga tcacccggct
      301 ggccgcgctc agccaggtgc agcagcgctt cgtgtccgac gtctcgcacg aactgcgcac
      361 gccgctgacc accatccgtc tcgcgggcga cgtgctctac gaccagcgcg agtcgttccc
      421 gcccgcgacc gcgcgcaccg ccgaactgct gcacacccag gtggaacgct tcgagctcct
      481 cctcgccgac ctcctcgaga tgagtcgcta cgacgccggc gccgtcgaca tggagaccga
      541 gcccaccaac ctcgtgcgcc tcgtcgagga cgccgtgcac acggtcgcgt ccctcgccga
      601 gtcgaagggc tccgagctgc gggtggttgc gccgggcggc tacttcgaag ccgaggtgga
      661 tgctcgtcgc atccgccgca tcatctccaa cctgctcggc aacgccatcg atcacggcga
      721 gggaaggccc atcgtcatct gggtcgacag cgacaaggag gcggtcgcga tcgccgtgcg
      781 cgactacggt gtcgggatga cggctgccgc aatggaacgc gtcttcgacc ggttctggcg
      841 cgccgacccg tcccgccaac gcaccaccgg cggcacggga ctgggcctgg cgatctccct
      901 cgaggacgca gcgctccacg gcggctggtt gcaggtgtgg tcggccccgg gcgaggggtc
      961 gtgcttccgg ctcacgcttc cgcgggtgcg cggtgcggtt ctgaagtcgt cgccgctgga
     1021 gctaccgcca ccggaggaga cggcgacgtt gggggtgctc gatgcgtagg gcgctcatcc
     1081 tgggattcgt cgtctgcctc gcgctcacgg gctgtgtcgg catccccacc tcgggcggag
     1141 tcgagaccgg tccggtcatc gacgaacagc tcgatcccga cttcgtcatc gacccgtccg
     1201 gtccgcgcgc cgggtcggct ccggaggaca tcctcaccga cttcatgctc gctctgcggg
     1261 ggccgcaggg caactatgag atcgctcgcg agttcctcac caaggacttc gcgacgaagt
     1321 ggtcacccga cgcgagcgcg acgatccgca ccggcatccc cgccaccacc ccgggggagg
     1381 cgccgaacac tctcgactac acgatcacca cccgcgccta cgtcgacgcc gacggtcgct
     1441 acttcgagcc ggggcctgcg acgcaggtgc tcacctactc cttcgtgcag gagaacgggg
     1501 agtggcgcat cagcggcgga cccgacggca tcgtgctctc ccagtcgagc ttctcggtcg
     1561 tgttcaccga gcgggcgctg tacttcttcg acccgaccta cacctacctg attcccgacg
     1621 tgcgctggtt cgcgtccagg gcgaccctgc ccgggtgggc cgtgcgtgag ctgctcgacg
     1681 ggcctgccca gtggctccag cagggcgtcg tgctcagcgc gttcccgatc tcgacgacgc
     1741 tcgacaccgc atccatcgac tcgggtgtcg cgaccgtgga gctgagcaag gaggcgctcg
     1801 cggccagcgc caccgaccgg gacaggatgc gccaacagct cgcggccacc ctcgacgtcg
     1861 ccaacgtggt gatgaccgtc agtggcattg acatctcggc gccggcgaca gggccgcagg
     1921 ccaccaagga tccgtcagtc gagcccgcga cgctcgtggg caccgacacc gagttcggct
     1981 tcgacacggg cgcgggcgtc gcacccctgg agggcctgag tgcgacggtg attgccgggg
     2041 gtgcgaccgc ggcaaccctg tccaacgacg agcagtacgt cgccttcttg gggccggacg
     2101 gcgtgtctgt agctcagcgc ggcaatgacc tcggcgaccc ggtggatgcc cggcccgggt
     2161 tggttgcccc gtcgatcgac cccttccgct tcgtgtggtc ggcgcaggct gagagcgccg
     2221 cgaccatcag caccttcgag atcgacggaa ccgagcacgc cctgacctcg agcgcgcccg
     2281 cggattccgc tgttgtctcc ctcgacgtct cgcgtgacgg ggcgcggctg ctgctctacc
     2341 tgtcgacgcc gctcgggccg cgactggcgg tggcgggcat catccgcgac cagaagaacg
     2401 tgccgacggc gctgggccag ctcgagtacc ttccggtggc gagcaatgcg ccgatcgatg
     2461 cgacctgggt cgacgcccgc accgtggcca cggtgtcgcg aggcggtgag gtcgcccccg
     2521 tgaccctgta cgagatcggc gggccgagcg ccacgttcgg ccaggtgccc gacgcctcga
     2581 cgatcgtcgg cggcaacggc ggcgcggatg gacttcgcgt gctgcgcacg tcgggagagg
     2641 tgctgcggcc gcagggcagc agctgggtca ccaccggcct ctccgcacgc gtgctcgcga
     2701 ccaagcagta gtccacgagt cgcgggctcg cgctgcgcgg tcgcagtgct ggcctcacgg
     2761 tgggtggatg ccgctgcgtg atgccctgct cgacgcgctg ctgagcgccc tgccccctgc
     2821 ccttcgcgat gccctgctcg acgcctggtc ggtgctgttc cccgtggact gcgcggggtg
     2881 cgacagcgct gaccgctccc tgtgcgacga gtgccgggcc gccctgcaac ccgacgtcac
     2941 cgtgcggtcg gtgggcgggc ctgggcccga cggccttggg gtgcacagcg gggcacgcta
     3001 cgaggggcgg gtgcggcgcg tcatcctcgc cttcaaagaa caggggcgca cggatgccgc
     3061 ggcagccctc tcccgccccc tcgccgccgc cgtcgcggca gcgctcagca cggctcccgc
     3121 cgccgagctc ctgctcgtgc ccaccagtcg cgccgcgtac cgccgcaggg gctacgaccc
     3181 ggttcggcta ctcgcgtccc gcgccggcct gcgggtcgcc cgggagttgc gtcaggtgcg
     3241 cgcgaccggc gcgcagaagg cgctcggcgt cgagcagcgg gcggccaatc gcgacggtgc
     3301 tttccgagcg cggcgcgatc tgaacggtcg gtcattcatc gtcgtcgacg acgtggtcac
     3361 ctcgggggcg acgattctcg aggtcgcgcg ggccgtcagg gcggccggtg gcgacgtgct
     3421 cggcgccgca acattggcat ttactccgag gttgttgccg atccgtgaca ttgccgaggg
     3481 gcaggagtac ggtaacgaca aggaggcgca taagaccgcc tgacagaccc ggcggccgcg
     3541 ctagtagcag gctggagggc gtcatggaca tcaccatcac cggacgaaac gttggggtta
     3601 cagaccgttt ccgggagtac gcgaccgaga aggccggcaa gatagaccac ctcgccgaca
     3661 aggcgatcgc gttcgaggtg aaggtggcca gacaccatga gacgcgaggc tccagcggcg
     3721 atgaccgggt ggagctgacg ctcatcggac cgggtccgtt ggtgcgggcc gagtcatccg
     3781 gttcagacaa gtacgtggca ttcgacctgg cgatggccaa gctggttgag cggatgcgtc
     3841 agtcgaagga tcgtcgcaag gtgcaccgtg gcaatcaccg ccccgtgtcg ctgcgtgagg
     3901 cgtcggccga cggattcagc atcgtcgaca tcaccccggc gagcgtcgag gtgctcgagc
     3961 gcgtcgcgac cggggagatc ccggtgcagg cggtggacgc cgccgaggag gagtactcgc
     4021 cggttgtcat ccgcgagaag gtgttcccat cgacgcacat gaccgtgagc gacgccgtcg
     4081 accacatgga actggtcggg cacgacttct tcctgttcat cgacgccgag accgaccgcc
     4141 cgagcgtcgt ctaccgccgc aagggctggg actacggggt gatcggcctc gaggagcagc
     4201 aggaggcgcc ggcgaagcgt tcgcgccgcg cgta
//
LOCUS       DAOSOU010000192         4229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21010_length_4229_cov_6.918783, whole genome shotgun sequence.
ACCESSION   DAOSOU010000192 DAOSOU010000000
VERSION     DAOSOU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21010_length_4229_cov_6.918783"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PLW15_09625"
     CDS             <1..171
                     /locus_tag="PLW15_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794147.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HOT34690.1"
                     /translation="LHHRTAPPTINLTEQDPEIPLDVVTSPRALGDGPLLALSNSFGF
                     GGHNSVAAFRSY"
     gene            complement(165..320)
                     /locus_tag="PLW15_09630"
     CDS             complement(165..320)
                     /locus_tag="PLW15_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34691.1"
                     /translation="MDTLNRELEELVDEAVPPLIDMTQLERLVTADDLVLFDGECTAH
                     PGCRPVQ"
     gene            complement(338..841)
                     /locus_tag="PLW15_09635"
     CDS             complement(338..841)
                     /locus_tag="PLW15_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HOT34692.1"
                     /translation="MTAPTARGVLYVHSAPRALCPHIEWAAGRALDRAVNFSWESQPV
                     LKGSQRAEFYWEGERGTGAKLASALRGWEHLRYEVTEDAGLGTDGGRWMHTPDLGIFF
                     AQTDTVGNVVIPEDRVRYAMEVAGANAIELHRELRLALGQAWDDELEVFRHAGQDSAV
                     VWLHKVG"
     gene            complement(1006..2646)
                     /locus_tag="PLW15_09640"
     CDS             complement(1006..2646)
                     /locus_tag="PLW15_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HOT34693.1"
                     /translation="MLIVLARVDGDRIMAARRSEDGGSFGIEYVKDLASFVAAEERAT
                     PRWVWDDTSHWYPRLLAERVRVDRCYDLRLCHAILRNSELTAGSALATGPATTWDAPR
                     VPDRPASGALFELEGDEPRVDSDPVAELVLQREAVAASAQPGRIDRLLAAESAGALIA
                     AELEYAGLPWDAARHEEILADLLGPRPADGERPAALREVLERVQQLLEAPGLNPDSPV
                     DLLRALGRVGLQVTSTRSWELKQLKHPAIGPLLEYKKLARLLSANGWNWLDTWVRDGR
                     FRPGYVPGGVVTGRWAANGGGALQLPRQIRGAVIADPGWKLVVADASQLEPRILAAMS
                     GDRGMVQAGAAGDLYAGIVASGAVETRDQAKVAMLGAMYGATTGESGRLMPRLERAYP
                     KAIALVEEAARAGERGEIVTTRLGRSSPRGQGAGYDEVASESEVSRARNRARAWGRFT
                     RNFVVQGSAAEWALCWMASIRRRLHTMADGTWLTDAPHLVFFLHDEIVVHCPAELTDA
                     VSEQVRLAAVEAGQLLFGAIPAEFPVTVAVVDNYGQAK"
     gene            complement(2651..3475)
                     /locus_tag="PLW15_09645"
     CDS             complement(2651..3475)
                     /locus_tag="PLW15_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HOT34694.1"
                     /translation="MTDTATETPESQLATYHERRELSVVQPLGSLALVNTQWVDSEQT
                     IYGVPGRWAPNPGGGLTVTAEASDAIVVDGELVDGTVVVRGKDDPNPSTIVFSDTVTG
                     FVIASSEGTYALRVWDSNSEDIQRFGGIDAYGYNPEWVITAKFSENPAGTTLGFEHLK
                     DDGQTREEVIPGEITFTKDGVDYNLAAFKAGRALQLVFADATNGDDTYSVGRFLFVAP
                     NPDGTITLDFNRAVLPPCAFSYNFNCPLPPKQNRFGVRIEAGEKNVLAKDGTLLHG"
     gene            3591..3782
                     /locus_tag="PLW15_09650"
     CDS             3591..3782
                     /locus_tag="PLW15_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOT34695.1"
                     /translation="MDPITISGGIIGLIIFIVTVVSIVNNPNHGGLGKFIWILVAFFL
                     SIIGSILWLIFGRGRVPKR"
BASE COUNT          640 a         1505 c         1392 g          692 t
ORIGIN      
        1 ctgcaccacc gcacggcgcc gcccacgatc aacctcacgg agcaggaccc cgagatcccg
       61 ctggatgtcg tcacctcgcc ccgcgcgctg ggagacggcc ctctgctcgc gctgagcaac
      121 tcgttcggct tcggcggcca caactcggtg gcggcgttcc gcagctactg aaccgggcgg
      181 caacccgggt gcgcggtgca ctccccgtcg aacagcacga ggtcatcggc ggtgaccagc
      241 cgctcgagct gagtcatgtc gatcagcggg ggcaccgcct cgtcgacgag ctcttccagt
      301 tccctgttca gcgtgtccat gacgactcct tcgtctctca acccaccttg tgaagccaca
      361 ccaccgctga gtcctgccca gcgtggcgga agacctcgag ctcgtcgtcc caggcctggc
      421 ccagggccag acgcagctcg cggtgaagct cgatcgcgtt ggctcccgca acctccatcg
      481 cgtagcgcac gcgatcctcg gggatgacga cgttgcccac cgtgtcggtc tgggcgaaga
      541 agatgccgag gtcgggcgtg tgcatccagc gaccgccgtc ggtgccgagg ccggcatcct
      601 cggtgacctc gtagcgcagg tgctcccagc ctcgcagcgc cgaggcgagc ttcgcgcccg
      661 tgccccgctc accctcccag tagaactctg cacgctggga acctttgagc accggctgcg
      721 actcccagga gaagttcacg gcacggtcga gtgctcggcc agcggcccac tcgatgtgtg
      781 ggcacaacgc acgaggagcg gagtgcacat acagcactcc gcgagcagtt ggtgcagtca
      841 cgtgctgtgc agacaaggct gcctccgttc cggttgggtg cgacttcccc atcgacccag
      901 tgaacggatg cccgtaacgg cggtattgcg tgtgaaattg gtgcggcgaa ccgctggggc
      961 cattattgcc gatgtcgagg gtgaatcaca agcgtgtaac tcgtgtcact tcgcttggcc
     1021 gtagttgtcg acgacggcca ccgtcaccgg gaactccgcc gggatggccc cgaagagcag
     1081 ctgtccggcc tccacggcgg cgagccgcac ctgctcgctc acggcgtcgg tgagctcggc
     1141 ggggcagtgc acgacgatct cgtcatggag gaagaacacg aggtgcgggg catccgtgag
     1201 ccaggtgccg tcggccatgg tgtgcaggcg ccgacggatg ctcgccatcc agcacagggc
     1261 ccactcggct gccgaaccct gcaccacgaa gttgcgggtg aaccgccccc aggctcgcgc
     1321 ccggttgcgt gcgcgggaca cctccgactc ggatgccacc tcgtcgtagc cggctccctg
     1381 gccgcgcggc gagctgcgac cgagtcgggt cgtgacgatc tcgccgcgct cgcctgcgcg
     1441 cgccgcctcc tccaccagcg cgatcgcctt cgggtaggcg cgctcgagcc gcggcatcag
     1501 gcggccactc tcgcccgtgg tcgctccgta catcgcaccg agcatggcga ccttcgcctg
     1561 gtcgcgcgtc tcgaccgcac cgctcgcgac gattcccgca tacaggtctc ccgcggcacc
     1621 cgcctgcacc atcccccggt cgcccgacat ggccgcgagg atgcgcggtt cgagctgcga
     1681 ggcatccgcc accacgagct tccagccggg atcggcgatc accgcgcccc ggatctggcg
     1741 cggcagctgc agcgctcccc caccgttggc ggcccagcga ccggtgacca ccccgcccgg
     1801 cacgtacccg gggcggaatc gcccgtcgcg cacccaggtg tcgagccagt tccagccgtt
     1861 ggccgagagc aggcgcgcga gcttcttgta ctccagcagc gggccgatcg ccggatgctt
     1921 cagctgcttc aactcccacg atcgcgtcga cgtgacctgc agcccgaccc tgccgagggc
     1981 acgcagcagg tccaccggcg agtccggatt gaggccgggc gcctcgagca gctgctgcac
     2041 ccgctccagc acctcgcgca gcgcggcggg ccgttcgccg tccgccggtc gcggaccgag
     2101 cagatcggcg aggatctcct cgtgccgcgc cgcgtcccac ggcagtccgg cgtactccag
     2161 ctcggcggcg atcagcgcgc cggccgactc cgcggccagc agccggtcga tccgtcccgg
     2221 ctgcgccgat gccgcgaccg cttcgcgttg cagcacgagt tcggcgaccg ggtcggagtc
     2281 gactcgcggc tcgtcgccct cgagttcgaa gagcgctccg ctggccgggc ggtcgggcac
     2341 ccgcggcgca tcccacgttg tcgcggggcc ggtggccagg gcggagcccg ccgtcagctc
     2401 cgagttgcgc aggatcgcat ggcacagccg caggtcgtag cagcggtcga cgcgcacccg
     2461 ctcggccagc agtcgcgggt accagtggga cgtgtcatcc cacacccacc ggggcgtcgc
     2521 gcgctcctcg gccgcgacga acgacgcgag atccttgacg tactcgatgc cgaatgaccc
     2581 gccgtcctcg ctgcgccggg ccgccatgat gcggtccccg tcgactctgg cgagaacaat
     2641 gagcatgatc tcagccgtgc agcagtgtgc cgtccttggc gagcacgttc ttctctccgg
     2701 cctcgatgcg cacgccgaag cggttctgct tgggcggcag cgggcagttg aagttgtagc
     2761 tgaaggcgca gggcggcaga actgcccggt tgaagtcgag agtgatcgtg ccatccgggt
     2821 tcggcgcgac gaacaggaac cgacccacgc tgtaggtgtc gtcgccgttg gtcgcgtcgg
     2881 cgaacacgag ctgcagcgca cggccggcct tgaaggcggc caggttgtag tcgacgccgt
     2941 ccttggtgaa ggtgatctcg ccggggatga cctcttcgcg ggtctgcccg tcgtctttga
     3001 ggtgctcgaa accgagggtg gtgccggccg ggttctcact gaacttcgcg gtgatgaccc
     3061 actccgggtt gtagccgtac gcgtcgatgc cgccgaagcg ctggatgtcc tccgagttcg
     3121 agtcccacac ccgcagggcg taggtgccct cggagctggc gatgacgaag ccggtgacgg
     3181 tgtcgctgaa cacgatcgtg ctcgggttcg ggtcatcctt gccgcgcacc acgaccgtgc
     3241 cgtcaacgag ctcgccgtcg acgacgatcg cgtcggatgc ctcagccgtg accgtgaggc
     3301 cgccgcccgg gttcggcgcc catcgccccg gaacgccgta gatcgtctgc tcggagtcca
     3361 cccactgggt gttgacgagg gcgaggctgc cgagcggctg cacgacgctc agttcgcgcc
     3421 gctcgtgata ggtggcgagc tggctctcgg gcgtttcggt ggcggtgtcg gtcatgggtt
     3481 ccatcctcgt gtgatctacc gactaaaccg gcggcgacac gggtttatgc ccggcgtcga
     3541 acccctaaac tgttgtcgcg cagaccggcg cctcatgcga aaggcagcac atggacccga
     3601 tcaccatcag cggcggcatc atcggcctca tcatcttcat cgtcactgtg gtgtcgatcg
     3661 tgaacaaccc gaatcacggc ggcctcggca agttcatctg gattctggtc gcgttcttcc
     3721 tgtcgatcat cggctcgatc ctctggctga tcttcggccg cggtcgtgtg cccaagcgct
     3781 gacgcccggt accactccca catccggtcg cacgcctcct cgagcgtcca ggcgtacccg
     3841 agcaggcgcc gctccggcgt gagtccacgc aggcccgcgc ggtggttgat gcggccgtac
     3901 tcgatgatcc cgatcagcgt gccgcggtcg tagagccgcc actcgccggg ccggaacgag
     3961 tgctcgaggt tgtcgctcgc gcgccagtga tgcaccatgc ttcgaacata cattcgattg
     4021 gcgctgcctg tgaatacccc ggcgcatccc gagcaacact cgacataggc tgagagcatg
     4081 tcgagccagt ggaccgagac cgccgcccag tacgtcacga gcacggcatc ctgtccgcgg
     4141 tgcgacacca ccctggctca gcccgggcac tgcaccgagt gcggtgccga tctggccggc
     4201 ccccaggcca aggcggtgtg gaacacgtc
//
LOCUS       DAOSOU010000193         4223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21051_length_4223_cov_4.934981, whole genome shotgun sequence.
ACCESSION   DAOSOU010000193 DAOSOU010000000
VERSION     DAOSOU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21051_length_4223_cov_4.934981"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PLW15_09655"
     CDS             complement(<1..175)
                     /locus_tag="PLW15_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HOT34696.1"
                     /translation="MRLSEFWLAVSDEFGPEYGRVLTSDLVLGEIGGLTAQQGIAKGV
                     APREIWLALCEAMD"
     gene            complement(257..580)
                     /locus_tag="PLW15_09660"
     CDS             complement(257..580)
                     /locus_tag="PLW15_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT34697.1"
                     /translation="MVLVRQEIGDVLRDFRLQKGRTLRQVASKASVALGYLSEVERGQ
                     KEASSEILASVAEALETPISVIMREVGDRLAVIEGLNPIPETHIPDTLPDELVAQFDS
                     DLVSR"
     gene            complement(740..1219)
                     /locus_tag="PLW15_09665"
     CDS             complement(740..1219)
                     /locus_tag="PLW15_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOT34698.1"
                     /translation="MTTSAEIIATLIERGYQIAVAESLTGGLLVAELIDTAGASQVVV
                     GGVVAYNTELKASVLNVDAGALAEHGAVHPDVAAQMATGVRAALALAAGPPHIGISTT
                     GVAGPEPQDGQSVGTVYLGFAIGGDVSVKRLSLTGSRADIRSATVSEALSELARLLR"
     gene            complement(1216..1809)
                     /gene="pgsA"
                     /locus_tag="PLW15_09670"
     CDS             complement(1216..1809)
                     /gene="pgsA"
                     /locus_tag="PLW15_09670"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOT34699.1"
                     /translation="MKGRVASHGDTKASNGNVANIITVARILLAPVFVWLLAADNGEH
                     GVLRWVAAGLFILAIATDGVDGSLARSRNLVTNVGIILDPIADKVLTGAALIMLSVLG
                     ELWWWVTIVILVRELGITAFRFAVLRSRVIPASRGGKLKTIVQSVAISLFLVPLNTLV
                     GDWVLWVNWVAMGAALVLTVVTGIDYLWQAWRGNRSK"
     gene            complement(1900..>4223)
                     /locus_tag="PLW15_09675"
     CDS             complement(1900..>4223)
                     /locus_tag="PLW15_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOT34700.1"
                     /translation="IWLFRHPSSVHDNTRIGIGVTLLITTISGLCHIFGGQPQPTQGM
                     ESLARAGGIVGWVLASPLLYVGTAVFAAPVVILLLVLSLFIITKTPPNRIGSRLRELY
                     AYLFGAQLPDESERAAAKAARNDSVEFGSLSDLGIDEDPASIPWWRRNKAPSAGEPAF
                     DSPVISREPATEVIAPVPSSPVAEDEFGIDLLEDLLKAEEAVKRFTGEVETGATGLRE
                     DGPGLLPGFVTKASEKGEAGEFDGAVPARPKQPTAVYRMPAASLLTPGTPPKKHSAAN
                     DEVVAAITEVLKQFQVDAQVTGFSRGPSVTRYELELGPGVKVERVTALARNISYAVAS
                     NEVNILSPIPGKSAIGVEIPNKDREIVSLGDVLRSGASTSSLHPMTIGLGKDVEGGFV
                     VANLAKMPHLLVAGSTGSGKSSFVNSMITSILMRAKPAEVRMVLIDPKRVELSIYAGV
                     PHLITPIITNPKKAAEALAWVVKEMDMRYDDLASFGFRHIDDFNRAVVAGEIVLPAGS
                     ERKLSPYPYLLVVVDELADLMMVAPRDVEDSIVRITQLARASGIHLVLATQRPSVDVV
                     TGLIKANVPSRLAFAVSSMTDSRVILDQPGADKLIGQGDALFLPMGASKAIRVQGAWV
                     SEAEVNAVVQHVIAQARPEYREDVAAEATRKQIDADIGDDLELLLAAAELVVSTQFGS
                     TSMLQRKLRVGFAKAGRLMDLLESREIVGPSEGSKARDVLVTAEQLPGVLARLRGADD
                     APVARDAVEESLSGYDVVDGDSDEDAWKLTDRE"
BASE COUNT          724 a         1409 c         1398 g          692 t
ORIGIN      
        1 catccatcgc ctcgcagagg gcgagccaga tctcgcgggg cgcaacgccc ttggcaatgc
       61 cctgctgtgc cgtgagtccg ccgatctcgc ccagcaccag gtcgctcgtg agtacgcggc
      121 cgtactccgg cccgaactcg tcggacacgg ccagccagaa ctcgctcagt cgcaccccac
      181 caaactaacc cgaaacgcac gaatgccccg gcgctcggcc ggggcattcg gtggacgctg
      241 gatcggcagc ggccgatcag cgagagacga ggtcggagtc gaattgggct acaagctcgt
      301 caggaagcgt gtcagggatg tgggtttcgg ggatcgggtt gagcccctcg atgacggcga
      361 ggcggtcgcc cacctcgcgc atgatgaccg agatcggggt ctcgagcgcc tccgctacag
      421 acgcgagaat ctcagagctg gcctctttct ggccgcgctc cacctcactg aggtagccga
      481 gcgcaacgct cgccttgctc gcgacctggc gaagggtgcg gcccttctgc aaacggaagt
      541 cacggagaac gtcaccgatt tcctgtcgta ccagaaccat tggaccctcc ttttctgcct
      601 gttgaatccg gccagttttc ccgttggagg cctaagacta cagcctctcg attatgagaa
      661 ctctaaccac tgacaactga gcagtacgtg tgaactcacc acatgtaacg agatcgaaac
      721 gagcagtatt cccgctcggt cagcggagca gtcgtgcgag ctctgatagt gcttcggaga
      781 cggtggccga acggatgtca gcacgacttc ccgtgaggct gagccgcttc acgctcacgt
      841 cgccgccgat cgcgaagccg aggtacacgg tgcccacgct ctgaccgtcc tgcggctcag
      901 gacccgccac cccggtcgtg gagataccga tgtgcggggg ccctgcggcg agagcgagcg
      961 cggcccgcac ccccgtggcc atctgcgctg cgacatccgg atgcacggca ccgtgttccg
     1021 cgagggctcc ggcgtccacg ttcagcacgc tggccttgag ctcggtgttg tacgcgacga
     1081 cgccacccac cacaacctgg gacgcccccg cagtgtcgat cagttcggcc acgagaagcc
     1141 cgccggtcag agactcggcg accgcgatct ggtacccgcg ctcgatgagc gtggcgatga
     1201 tctcggcgga ggtcgtcact tcgaccggtt gccccgccag gcctgccaga ggtagtcgat
     1261 gcccgtcacg acggtgagca cgagcgcagc gcccatggcg acccagttaa cccacagaac
     1321 ccagtcgccg acgagagtgt tcagcggcac gaggaagagt gagatcgcca ccgactgcac
     1381 gatggtcttg agcttgccac cgcgggacgc ggggatgact cggctgcgca gcacggcgaa
     1441 ccggaacgcg gtgatgccga gctcgcgcac gaggatgacg atggtcaccc accaccagag
     1501 ctcgccgagc accgagagca tgatgagggc agcgccggtg agcaccttgt cggcgatcgg
     1561 gtcgaggatg atgcccacgt tggtcaccag gttgcggctg cgcgcgagtg agccgtcgac
     1621 accgtcggtc gcgatcgcga ggatgaacag gcccgccgcg acccagcgca gcaccccgtg
     1681 ctccccgttg tcggcggcga ggagccagac gaagaccgga gcgagcagga tgcgggcaac
     1741 cgtgatgatg ttcgcgacgt tgccgttgga ggccttggtg tcgccgtgac tggccactcg
     1801 gcccttcatc gactccttga ccgcatcggc gcgcttggca tgcaccgacc tgagatccgg
     1861 tgactcctgg gggacttccg actcaggaac cacgcgcgtc tactcccgat cggtcagctt
     1921 ccaggcgtct tcgtccgaat cgccgtctac gacatcgtaa ccgctcaaag attcctcgac
     1981 cgcgtcacga gcgaccggcg catcgtcagc gccgcgaagg cgcgcaagca ccccgggaag
     2041 ttgttcggcg gtcaccagca cgtcacgcgc cttggacccc tcggacggcc cgacgatctc
     2101 gcgcgactcg agcagatcca tgagcctgcc ggccttggcg aagccgaccc tgagcttgcg
     2161 ctggagcatc gaggtggatc cgaactgggt gctcaccacg agctcggcgg cggccaggag
     2221 cagttcgaga tcgtcgccga tgtcggcgtc gatctgcttg cgggtggctt cggcggcgac
     2281 atcctcgcgg tactccggcc gggcctgggc gatcacgtgc tgcacgaccg cgttgacctc
     2341 ggcctcggac acccacgcgc cctgcacgcg gatcgccttc gaggccccca tcggcaggaa
     2401 cagcgcgtcg ccctgcccga tgagcttgtc ggccccgggc tggtcgagga tgacgcggct
     2461 gtcggtcatg ctcgacaccg cgaacgccag cctcgacggc acgttggcct tgatcaggcc
     2521 ggtcaccacg tcaaccgacg ggcgctgggt cgcgagcacc aggtggatgc ctgacgcccg
     2581 ggccagctgg gtgatgcgca cgatcgagtc ctcgacgtcg cgcggcgcga ccatcatgag
     2641 gtcggcgagc tcgtcgacga cgaccagcag atacgggtac ggcgacagtt tgcgctcact
     2701 cccggccggc agcacgatct cccctgccac caccgcccgg ttgaagtcgt cgatgtggcg
     2761 gaagccgaag ctcgccagat cgtcgtacct catgtccatc tccttcacga cccacgccag
     2821 cgcctctgcc gctttcttgg ggttcgtgat gatgggcgtg atgagatgcg gcacgcccgc
     2881 gtagatggag agttcaacgc gcttggggtc gatgagcacc atgcgcacct cggcgggctt
     2941 ggcccgcatg aggatggagg tgatcatcga gttgacgaag ctcgacttgc ccgagccggt
     3001 ggagccggcc acgagcagat gcggcatctt ggcgaggttg gcgacgacga agccgccctc
     3061 gacgtccttg ccgagcccga tggtcatggg gtggaggctc gatgtgctcg cgcccgagcg
     3121 gagcacgtca ccgagcgaaa cgatctcgcg atccttgttg gggatctcga caccgatcgc
     3181 gctcttgccc gggatcggcg acaggatgtt gacctcattg ctcgcgaccg catacgagat
     3241 gttgcgagca agagcggtga cccgctcgac cttgactccc gggccgagct cgagctcgta
     3301 gcgggtgacc gaggggccac ggctgaatcc ggtgacctgc gcgtcgacct ggaactgctt
     3361 gagcacctcg gtgatggccg ctaccacttc gtcgttggcg gcagagtgct tcttgggcgg
     3421 cgttcccggc gtcagaaggg atgccgcggg catccggtac acggccgtgg gctgcttcgg
     3481 cctggccggc accgcgccgt cgaactcgcc cgcctcgccc ttctcgctcg ccttggtcac
     3541 gaacccgggc agcagaccgg gaccgtcctc gcggaggccg gtcgcgccgg tctcgacctc
     3601 gcccgtgaag cgcttgaccg cctcttccgc cttgaggagg tcctcgagca ggtcgatgcc
     3661 gaattcgtcc tccgcgacag gagacgaggg caccggtgcg atgacctcgg tcgcgggctc
     3721 gcgtgagatc accgggctgt cgaaggccgg ctcccccgcg ctcggggcct tgttgcgccg
     3781 ccaccacggg atgctcgcgg gatcttcgtc gatacccaga tcgctcaggc tgccgaactc
     3841 gaccgaatca ttgcgggcgg cctttgcggc agcgcgctcc gactcgtcgg gcagctgagc
     3901 gccgaagagg taggcgtaga gctcgcgcag gcgagatccg atgcgattcg gcggcgtctt
     3961 ggtgatgatg aagagggaga gcaccagcag aaggatgacc accggtgcgg caaacaccgc
     4021 ggttccgacg tacagcagcg ggctggcgag cacccacccg acgattccgc cggccctcgc
     4081 gagcgactcc atgccctgcg tgggctgcgg ctgaccaccg aagatgtggc acaaaccgct
     4141 gatggtcgtg atgagcagcg tgacgccaat gccgatgcgg gtgttgtcgt gaacgctcga
     4201 cggatgcctg aacagccaga tcg
//
LOCUS       DAOSOU010000194         4211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21127_length_4211_cov_5.424928, whole genome shotgun sequence.
ACCESSION   DAOSOU010000194 DAOSOU010000000
VERSION     DAOSOU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21127_length_4211_cov_5.424928"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLW15_09680"
     CDS             complement(<1..459)
                     /locus_tag="PLW15_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34701.1"
                     /translation="MGSRWGRVARGWSAAAFATTVAAVSHTLAGGLAPSVFGIVASLV
                     ISGLVGTALAGSRLSSLRLAATMAISQLLFHGLFSSLGTPVSVPHSHSDMTIGLDAPA
                     HHHAGTMWLAHAAAGLIAFLAYRFGEAAFFGLATTAALLLARLTRVLVPVV"
     gene            647..1924
                     /gene="clpX"
                     /locus_tag="PLW15_09685"
     CDS             647..1924
                     /gene="clpX"
                     /locus_tag="PLW15_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT34702.1"
                     /translation="MARIGESADLLKCSFCGKSQKQVQQLIAGPGVYICDECVELCNE
                     IIEERLAEAGEEVSSEFDLPKPREIYAFLEEYVIGQEHAKRALSVAVYNHYKRIRARN
                     TITSADAVIDDVEIAKSNILLIGPTGCGKTYLAQTLAKRLNVPFAVADATALTEAGYV
                     GEDVENILLKLIQAADYDVKRAETGIIYIDEIDKIARKAENPSITRDVSGEGVQQALL
                     KILEGTVASVPPQGGRKHPHQEFIQVDTTNVLFIVAGAFAGLDEIISQRAGKKGIGFG
                     APLHSKGDDVNLFSEVLPEDLHKFGLIPEFIGRLPVVTTVTPLDQVALMDILTKPKNA
                     LVRQYQRMFEIDGVELDFDHEALEAIADLAVLRQTGARGLRAIMEEVLGPIMFDVPSD
                     DQVARVVVTRAAVLENAAPTIVPRSAPRAEKSA"
     gene            2010..3194
                     /locus_tag="PLW15_09690"
     CDS             2010..3194
                     /locus_tag="PLW15_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HOT34703.1"
                     /translation="MSAGFVAAISGFASSFVLVIAGLQAVGASSAEAASGLFAVCIAV
                     AITNVVAALTLRIPLAFAWSTPGAAVLLAAATPGLKFSDAVGAFLVSAALMLLCGLWP
                     LLGRAITRIPRPLASALLAGVLLPICLKPVEAVATLPLLAAPIVIVWLVLYRLAPRWA
                     VPAAMVVAVVSILIAAGTDWVGEAAIAPQLTFVAPTFDLFTILSVGLPLFVVTMAGQN
                     VPGFAVMSANGYVAPPRFALVSGGLGSGVAALFGGHAINLGAITAAIMAGPESHTDKD
                     RRWIATLTNGILYVPLGAGAGLAAALITAAPAILVTAVAGLAVLGALITSIVNALEQP
                     EHRVAAIVTFVVVVSGIVIAGIGSALWGLAAGGLVMAWLGWRRRPTPTVPSFEPSSDS
                     DE"
     gene            3209..3949
                     /locus_tag="PLW15_09695"
     CDS             3209..3949
                     /locus_tag="PLW15_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34704.1"
                     /translation="MSATSSTPPALRELRRTAVIFIIVSLSLTALVGIVTLLTASFGE
                     VQAKVILTTLLVAGFSITALCHLAVVGRALRVVGFVGIAASGLALITGVVLIWGSWDN
                     WNSGWEALLKSFAVLGVLSLSLAHANLLLLLGDRPSPLIRYGLITTVALIGLLALLII
                     LPIVSDGRIPGDNDVYWRILGVVAILDVLGTIVLPVVSRFTTGGRAASVTIRLHGDAA
                     ARLTRIATERGSTPAAVVEAAVADLPEA"
     gene            complement(3946..>4211)
                     /locus_tag="PLW15_09700"
     CDS             complement(3946..>4211)
                     /locus_tag="PLW15_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOT34705.1"
                     /translation="RYSSTYFAHTFSGGYDAGYYSYIWSEVLDADTVEWFKENGGLTR
                     ANGDRFRQRLLGVGGSKDPLEAFRDFRGRDADIQPLLDRRGLN"
BASE COUNT          644 a         1466 c         1381 g          720 t
ORIGIN      
        1 caccacgggc accagcaccc gggtgaggcg agccagcagg agtgcggccg tggtggccag
       61 cccgaagaac gcggcctcac cgaagcggta ggcgaggaag gcgatgagcc ccgcggcggc
      121 gtgtgccaac cacatggtgc cggcgtggtg gtgcgccggg gcatccagtc cgatggtcat
      181 gtcggagtgg gagtgcggca ccgacaccgg cgtaccgagg ctcgagaaca ggccgtggaa
      241 cagcaactgg ctgatcgcca tggtcgccgc gagtcgcagg gacgacagcc ggctgccggc
      301 gagggcggtg cccacgagac ccgagatcac caaggatgcg acgattccga acacgctcgg
      361 cgccagccca ccggcgaggg tgtgagacac ggcggcgaca gttgtcgcga acgcggcggc
      421 gctccacccg cgggcgactc gcccccaccg tgaacccatc cgcatcgtgt cgatctcccg
      481 tcgtgaccat cgtgccacac cgccaatggc ggcccgaaat cgccgggaat gggccgcggc
      541 gcgccgagac gcgaagcgcc gcactgcccg ccaccgtccg ccgatcgggt taggctcggt
      601 gtgttgcatc gacagcacac caccgcgtgg ggaagaggtt ccgctcatgg ctcgcatcgg
      661 cgaaagcgct gacctcctca agtgttcctt ctgcggcaag agccagaagc aggtgcagca
      721 gctcatcgcc ggtcccggcg tctacatctg cgacgagtgc gtcgaactgt gcaacgagat
      781 catcgaggag cgtctcgccg aggcgggcga agaggtgtcg agcgaattcg acctgccgaa
      841 gccccgtgag atctacgcgt tcctcgagga gtacgtcatc gggcaggagc acgccaagcg
      901 cgccctctcg gtcgccgtct acaaccacta caagcgcatc cgtgctcgca acaccatcac
      961 gtcggccgac gccgtcatcg acgacgtcga gatcgcgaag agcaacatcc tgctgatcgg
     1021 ccccaccggc tgcggcaaga cctatctggc gcagacgctc gcgaagcgcc tcaacgttcc
     1081 cttcgccgtc gcggatgcca cagcactcac cgaggccggc tacgtcggtg aagacgtgga
     1141 gaacatcctc ctcaagctca tccaggccgc cgactacgac gtcaagcgcg ccgagaccgg
     1201 catcatctac atcgacgaga tcgacaagat cgcgcgcaag gccgagaacc cgtccatcac
     1261 ccgtgacgtg tcgggcgagg gtgtgcagca ggcgctgctg aagatcctcg agggcacggt
     1321 cgcgtcggtt ccgccgcagg gtggccgcaa gcatccgcac caggagttca tccaggtcga
     1381 cacgaccaac gtgctgttca tcgtggcggg cgccttcgcg ggcctcgacg agatcatctc
     1441 ccagcgagcg ggcaagaagg gcatcggctt cggtgccccg ctgcacagca agggtgacga
     1501 cgtgaacctc ttcagcgagg tgctgccgga ggacctccac aagttcggac tcatccccga
     1561 gttcatcggc cgtctgcccg tcgtcacgac tgtgacgccg ctcgaccagg tcgccctcat
     1621 ggacatcctc accaagccga agaacgcact cgtgcgccag taccagcgca tgttcgagat
     1681 cgacggcgtc gagctcgact tcgatcacga ggccctcgag gccatcgcgg acctggcagt
     1741 gctgcgtcag accggtgcgc gcggcctgcg cgccatcatg gaggaagtgc tcgggccgat
     1801 catgttcgac gtgccctccg acgaccaggt cgctcgcgtc gtcgtgacgc gtgcggccgt
     1861 gctcgagaac gccgcgccga cgatcgtgcc gcgcagcgcg ccgcgtgcgg agaagtccgc
     1921 ctagccacgt gccctcgatt ggcccgcact agggcctctc caagcgccct aggctggaag
     1981 gcatcgacag gccatccgtt ctccagccga tctcggcggg gttcgtcgct gcaatctctg
     2041 gcttcgccag ttccttcgtt ctggtgatcg ccggactgca ggccgtcggg gcgagctccg
     2101 ccgaggcggc ctcgggtttg ttcgcggtct gtatcgccgt cgcgatcacc aatgtcgtcg
     2161 ctgccctgac cctgcgcatc ccgctcgcat tcgcatggtc gactcccgga gctgccgtgc
     2221 tgctggctgc agccaccccg ggcctgaagt tctcggatgc cgtgggggct ttcctcgtgt
     2281 cggcagcact catgctgctg tgcggtctgt ggccgctgct gggtcgcgcc atcacccgga
     2341 ttcctcggcc gctggcgagt gcgctgctcg cgggcgtgct gctgccgatc tgcctcaagc
     2401 cggtcgaggc ggttgcgact ctgccgctgc tggccgcgcc gatcgtgatc gtctggcttg
     2461 tgctctaccg tctcgcccct cggtgggccg tgcctgccgc gatggtcgtc gcggtcgtgt
     2521 cgatcctcat tgcggccggg accgactggg tcggcgaggc ggcgattgcg ccgcagctga
     2581 ccttcgtcgc gccgacgttc gacctgttca ccattctcag tgtgggtctg ccgctgttcg
     2641 tggtgacgat ggcgggccag aacgttcccg ggttcgcggt gatgagcgcc aacggctacg
     2701 tcgcaccgcc gcgcttcgcc ctcgtctcgg gcggcctcgg ctccggagtt gcggccctgt
     2761 tcggcgggca cgcgatcaat ctcggcgcga tcactgcggc gatcatggcc gggcctgaat
     2821 cgcacaccga caaggaccgg cgctggatcg ccaccctgac caacggtatc ctctacgttc
     2881 ccttgggggc cggtgcggga ctcgccgccg ccctcatcac cgcggccccg gcaattctcg
     2941 tgaccgcagt cgccggactc gccgtcctgg gcgccctgat caccagcatc gtgaatgcgc
     3001 tcgagcagcc tgagcaccgg gtcgcggcga tcgtgacctt cgtggtggtg gtatcgggca
     3061 tcgtgatcgc gggcatcggc tcggccctgt ggggcctcgc agcgggaggc ctcgtgatgg
     3121 cctggctggg ctggcgtcgg cgtcccacac cgactgtgcc gtcatttgaa ccctcctcgg
     3181 attcggacga gtgacctagg ctgacgacat gtcagctacc tcgagcactc cgcccgcact
     3241 gcgggagctc cgccgcaccg ccgtgatctt catcatcgtg tcgctgtcgc tgacggctct
     3301 cgtgggcatc gtcaccctgc tcacggcctc gttcggcgag gtccaggcga aggtcatact
     3361 caccaccctg ttggtggccg gattcagcat cacggcactg tgccatctcg ccgtcgtggg
     3421 cagggctctt cgagtcgtgg gcttcgtggg catcgccgcg tcggggctgg cactcatcac
     3481 gggagttgta ctcatctggg gcagttggga caactggaac agcggctggg aggcccttct
     3541 caagtccttc gccgtgctcg gcgttctctc gctgagcctc gcccacgcga accttctgct
     3601 gctactgggc gaccgtccgt cgcccctcat ccgctacgga ctcatcacca ccgtcgcgct
     3661 gatcggcctg ctcgcgctgc tcatcatcct gccgatcgtc agcgacggcc gcatccccgg
     3721 tgacaacgat gtgtactggc gcatccttgg cgtcgtggcg atcctcgacg tgctgggcac
     3781 gatcgtgctg cccgtcgtga gccgattcac cactggcggg cgcgcggcat cagtgacgat
     3841 ccgcctgcac ggggatgccg cggcacgact cacccgcatc gccaccgaac gcggctcgac
     3901 tcccgccgcc gtcgtcgagg cggcggtcgc ggacctcccc gaggcctagt tcagtcctcg
     3961 ccgatcgagc agcggctgga tgtcggcgtc gcggccccgg aagtcgcgga acgcctcgag
     4021 cgggtccttc gacccaccca cgccgagcag acgctggcgg aaccggtcac cgttcgcgcg
     4081 ggtgaggccg ccgttctcct tgaaccactc gacagtgtcg gcgtcgagca cctcgctcca
     4141 gatgtaggag tagtaacccg cgtcgtaccc gcccgagaag gtgtgggcga agtaggtgct
     4201 cgagtagcgc g
//
LOCUS       DAOSOU010000195         4191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21278_length_4191_cov_6.899420, whole genome shotgun sequence.
ACCESSION   DAOSOU010000195 DAOSOU010000000
VERSION     DAOSOU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21278_length_4191_cov_6.899420"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PLW15_09705"
     CDS             complement(<1..1424)
                     /locus_tag="PLW15_09705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HOT34706.1"
                     /translation="MVAALYRRYRPETFAELIGQSQVTDPLRTALRTDRVNHAYLFSG
                     PRGCGKTTSARILARCLNCAEGPTDTPCGVCPSCVELSRDGGGSLDVVEIDAASHNGV
                     DDARDLRERAVFAPARDRFKIFILDEAHMVTPQGFNALLKIVEEPPEHVKFIFATTEP
                     EKVIGTIRSRTHHYPFRLVPPAQMLDYVQTLCDSEKVTVEPGVLPLVVRAGGGSVRDT
                     LSLLDQLMAGSEGDVVDYERAVALLGYTHGALLDEVVDALGARDAAAAFGSVDRVIQT
                     GQDPRRFVEDLLERLRDLIIVAATSGNAQAVLRGVPQDQLDRMIQQATTFGTAELSRA
                     ADVVNAALSEMTGATSPRLHLELMVARMLVPSSDDTERGALARVERLERRIGVEQDAA
                     APAARVTPARPAVELVETPPAKAPPGKTPKAAMPEVSTSSTTGRPAAEATVSTSSTTD
                     TSTTDSPTTAPVAAKAAGEVTLEQL"
     gene            complement(1487..2611)
                     /locus_tag="PLW15_09710"
     CDS             complement(1487..2611)
                     /locus_tag="PLW15_09710"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT34707.1"
                     /translation="MTLAFVVNSGSSSIKWELLEVESGESVRRGIVERIGEAGGDAAD
                     HRQAMTSIIGMLGAEHPVVIGHRVVHGGDLFSAATVVTPEVEAAIEKLSALAPLHNPP
                     NLAGIRAAQVAFPGIPNVAVFDTAFHQSLAPAAYTYAIDARVAAEHSVRRYGFHGTSY
                     QYVSRRAAEVIGRPLDELRLIVFHLGNGASACAIAGGRSVDTSMGMTPLEGLVMGTRS
                     GDLDPGVLFHLAREAGLTTPELDILLNRRSGILGLSGHGDMRDLVDTMKAGDAASRLA
                     FDVYVHRLRHYLGAYLAQLGGVDAIVFTAGVGENSSDVRAGALAGLGGLGIHLDAARN
                     ASRSPEPRRISTDDSPVAVLVVPTNEELEIARQSLAALAG"
     gene            complement(2608..>4191)
                     /gene="pta"
                     /locus_tag="PLW15_09715"
     CDS             complement(2608..>4191)
                     /gene="pta"
                     /locus_tag="PLW15_09715"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT34708.1"
                     /translation="AWAIPEDPYLVAPTMSAVMGAVDGRLLKGDEALLAREVLGIVIA
                     GMSMENVLPRIMEGALVVIAGDRSEMLLAALTAHASGTFPSLAGIVLNGGFELSPIID
                     RLIAGLDETLPIVTTDFGTYETAQRIVQTRGRLTATAQRKIDTALALFDKHVDAELLL
                     RELGAGESSAVTPLMFEYGLLERARSVPTRIVLPEGGDDRILQAAATLLSRAVVDITI
                     LGDEQEIRARASGLGVDVSAAQVLSPFDPELHEKFAAEYARLRAHKGMTIEAARDIVT
                     DVSYFGTMMVHFGLADGMVSGAAHTTAHTIKPALEFIKTTDGVSIVSSVFFMALEDRV
                     LVYGDCAVVPDPTAPQLADIAISASQTARQFGIEPRVAMLSYSTGESGTGADVDKVRE
                     ATAIVRERMPDLPLDGPIQYDAAADVAVAKAKLPDSQVAGRATVFIFPDLNTGNNTYK
                     AVQRSSGAVAIGPVLQGLRKPVNDLSRGALVRDIVNTVAITAIQAQSVVEPPAVVEPP
                     AVVEPPAVVEPVETPAENS"
BASE COUNT          635 a         1392 c         1530 g          634 t
ORIGIN      
        1 agctgctcga gggtgacctc acccgcggcc ttcgcggcga cgggtgcggt ggtagggctg
       61 tcggtggtcg aggtgtcggt ggtcgagctt gtcgagaccg tcgcctcggc ggccggacgt
      121 ccggtggtcg agcttgtcga gacctcaggc atcgctgcct tgggggtctt cccgggcgga
      181 gccttcgctg gcggggtctc gacgagctcg accgccggtc tcgcgggcgt tacgcgggcg
      241 gcgggagccg cggcatcctg ctcgacaccg atgcgccgct caagacgctc gacgcgggcc
      301 agagcaccgc gttcggtgtc gtcgctcgac ggcacgagca tgcgcgcgac catgagctcg
      361 aggtgcagcc gcggcgaggt cgcgcccgtc atctcgctga gcgcggcgtt gacgacgtcg
      421 gccgcccgcg acagctcggc ggtgccgaag gtggtggcct gctggatcat gcggtcgagc
      481 tgatcctgcg gcacgccgcg cagcacggcc tgggcgttgc ccgaggtggc ggccacgatg
      541 atgaggtcgc ggaggcgctc gagcaggtcc tcaacgaagc gacggggatc ctggccggtc
      601 tggatgacgc gatcgaccga gccgaacgcc gcagcggcat cccgtgcacc gagcgcgtcg
      661 acgacctcgt cgagcagcgc gccgtgggtg tagccgagca gggcgactgc ccgctcgtag
      721 tcgacgacgt caccctcgga gccagccatc agctggtcga gcagcgagag ggtgtcgcga
      781 accgagccgc cgccggcgcg cacgacgagc ggcagcacgc cgggctccac ggtgaccttc
      841 tcgctgtcgc agagcgtctg cacgtagtcg agcatctggg cgggcggcac cagccggaac
      901 ggatagtggt gggtgcgcga gcggatcgta ccgatgacct tctcgggctc ggtcgtggcg
      961 aagatgaact tcacatgctc cggcggctct tcgacgatct tgagaagcgc attgaacccc
     1021 tgaggcgtga ccatgtgcgc ctcgtcgagg atgaagatct tgaagcgatc gcgggcgggc
     1081 gcgaagacgg cgcgctcacg caggtcgcgg gcgtcatcca caccgttgtg gctggcggcg
     1141 tcgatctcga cgacgtcgag cgagcccccg ccgtcgcgcg acaactcgac gcagctcggg
     1201 cagaccccgc acggggtgtc agtcggaccc tcggcgcagt tcagacagcg ggcgaggatg
     1261 cgcgcgctcg tggtcttgcc gcacccgcgc ggaccgctga acagatacgc gtggttgacg
     1321 cgatcggtgc gaagcgcggt gcgcagcgga tcggtcacct gcgactggcc gatgagctcg
     1381 gcgaacgtct ctggccgata acggcggtac agggcggcaa ccacctgtca agagtagctg
     1441 ccgccagcga ccctggggcc gggtttcgat acgcgctgcg cgccactcag ccagccagcg
     1501 cggcgagcga ctggcgggcg atctccagtt cctcgttggt gggcacgacg agcactgcca
     1561 cgggcgagtc gtcggtgctg atgcggcgcg gctccggcga gcgcgatgcg ttgcgcgcgg
     1621 catccaggtg gatgcccagg cccccgagcc ccgcgagcgc ccccgcccga acgtcggaac
     1681 tgttctcgcc gacccccgcg gtgaacacga tcgcgtcgac gccgccgagc tgggcgaggt
     1741 aggcgccgag gtagtggcgt agccggtgca cgtagacgtc gaaggcgaga cgggacgcgg
     1801 catcccccgc cttcatggtg tcgaccaggt cgcgcatgtc accgtgtccg ctgagcccga
     1861 ggatgccgct gcgccggttg agcaggatgt cgagttcggg cgtggtcaat cccgcctccc
     1921 gcgcgaggtg gaacagcacg ccagggtcga gatcgccgga tcgggttccc atgacgagac
     1981 cctcgagcgg cgtcatcccc atggaggtgt ctaccgacct gcccccggcg atcgcgcagg
     2041 ccgatgcacc gttgccgaga tggaagacga tgagtcgcag ctcatcgagc ggtcgcccga
     2101 tcacttcggc cgcccgccgc gagacgtact ggtacgacgt gccgtggaag ccgtagcgcc
     2161 gcaccgagtg ctcggccgcg acccgtgcgt cgatcgcgta ggtgtaggcc gccggcgcga
     2221 ggctctggtg gaacgccgtg tcgaagacgg cgacattggg gatgccgggg aacgccacct
     2281 gcgcggcccg gatgccggcg aggttgggcg ggttgtgcag tggcgcgagg gccgacagct
     2341 tctcgatcgc cgcctcgacc tcgggtgtca ccacggttgc cgcggagaac aagtcgccac
     2401 cgtgcaccac gcgatggccg atgacgaccg gatgctcggc gccgagcatc ccgattatgg
     2461 aggtcatcgc ctgccggtgg tcggccgcgt cgccaccggc ttcgccgatg cgctcgacga
     2521 ttccgcggcg aacggactca ccggactcga cctcgagcag ctcccacttg atcgacgacg
     2581 agcccgagtt gacgacgaag gcgagggtca cgagttctcc gcgggggtct cgacaggctc
     2641 gaccaccgca ggcggctcga ccaccgcagg cggctcgacc accgcaggcg gctcgaccac
     2701 cgactgcgcc tggatcgccg tgatcgcaac ggtgttcacg atgtcgcgca cgagcgctcc
     2761 gcgcgacagg tcgttgacgg gcttgcgcag gccctgcagc accggcccga tcgcgacggc
     2821 acccgagctg cgctgcacgg ccttgtacgt gttgttgccg gtgttgaggt cggggaagat
     2881 gaacaccgtc gcgcggccgg cgacctgcga gtccggcagc ttcgccttgg cgacggccac
     2941 gtcggccgcg gcgtcgtact ggatcgggcc gtcgaggggg aggtcgggca tccgctcgcg
     3001 aacaatcgca gtcgcctcac gcaccttgtc gacgtcagcc cccgtgccgg actcgcccgt
     3061 cgagtacgac agcatcgcga cgcgcggctc gataccgaac tggcgcgcgg tctgcgaggc
     3121 cgagatggcg atgtcggcga gctgcggggc tgtcgggtcg ggcacgacgg cgcagtcccc
     3181 gtagacgagc actcgatcct cgagggccat gaagaagacg ctcgagacga tggagacgcc
     3241 gtcggtggtc ttgatgaact cgagcgcggg cttgatcgtg tgtgcggtcg tgtgcgccgc
     3301 gccggagacc atgccgtcgg ccaggccgaa gtgcaccatc atcgtgccga agtacgagac
     3361 gtccgtgacg atgtcgcgcg cggcctcgat cgtcatgccc ttgtgggcgc gcagcctcgc
     3421 gtattcggcg gcgaacttct cgtgaagctc ggggtcgaac gggctcagca cctgggctgc
     3481 ggagacgtcg acccccagac cgctcgctcg ggcacggatc tcctgctcgt cgccgaggat
     3541 cgtgatgtcg acgacagcgc ggctcagcag tgtggccgcc gcctggagga tgcggtcgtc
     3601 gccgccctcg ggaagcacga tgcgcgtcgg caccgagcgc gcccgctcga gcagcccgta
     3661 ctcgaacatg agcggagtga cggccgacga ctcccctgcg ccgagttcgc gcagtagcag
     3721 ttcggcgtcg acgtgcttgt cgaacagggc gagggcggtg tcgatcttgc gctgggctgt
     3781 ggcggtgagg cggccgcggg tctgcacgat ccgctgcgct gtctcgtagg ttccgaagtc
     3841 ggtggtcacg atcggcaggg tctcgtcgag cccggcgatg agtcggtcga tgatgggcga
     3901 gagttcgaat ccgccgttga gcacgatgcc cgccagcgac gggaacgtgc ccgacgcgtg
     3961 cgcggtgagg gcggcgagca gcatctcgct gcggtcgccc gcgatcacca cgagggcgcc
     4021 ctccatgatg cgcggaagca cgttctccat cgacatgccc gcgatcacga tcccgagcac
     4081 ttcgcgcgcc agaagcgcct cgtcaccctt cagcaggcga ccgtcgaccg cgcccatgac
     4141 ggcgctcatg gtgggtgcga ccagatacgg atcctcgggt atcgcccacg c
//
LOCUS       DAOSOU010000196         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21287_length_4190_cov_6.314631, whole genome shotgun sequence.
ACCESSION   DAOSOU010000196 DAOSOU010000000
VERSION     DAOSOU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21287_length_4190_cov_6.314631"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLW15_09720"
     CDS             <1..749
                     /locus_tag="PLW15_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT34709.1"
                     /translation="VSELSWKHIEHASEVVEVGQEVTVEILEVDLERERVSLSLKATQ
                     EDPWQVFARTHAIGQVAPGKVTKLVPFGAFVRVAEGIEGLVHISELSGKHVELAEQVV
                     SVGDEVFVKVIDIDLERRRISLSLKQANEGVDPEGTEFDPALYGMLTEYDDQGNYKYP
                     DGFDPETQEWKEGFEAQREKWEQEYAAAQARWEQHKKQVAAAALEEDHISDVPAGAST
                     FSSDEVSTGGGTLADDETLAALREKLSNNS"
     gene            complement(816..1421)
                     /locus_tag="PLW15_09725"
     CDS             complement(816..1421)
                     /locus_tag="PLW15_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOT34710.1"
                     /translation="MEFLTGTGLAVAAGLNAYIPLLILGLAGRFLDFVDLPANWAWLE
                     NPWVLGILGVLLVVEIVADKIPAVDSINDLFQTVVRPVAGGIAFGTGSASGTAVVTDP
                     AAFFTSGSWVPIAIGVVLALGTHLTKMAARPVLNVATAGTAAPAVSTAEDFGSVILSF
                     VALLFPLLVLVAIGGLVVALVAAVRRGRRRKRERVQDTSPL"
     gene            1464..2090
                     /gene="coaE"
                     /locus_tag="PLW15_09730"
     CDS             1464..2090
                     /gene="coaE"
                     /locus_tag="PLW15_09730"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT34711.1"
                     /translation="MYLIGLTGGIASGKSVVAKRLAEHGAVHVDADALAREVVEPGTE
                     GLAAIERAFGPQVIAPDGSLDRPALGAIIFPDAEKRALLNSITHPAVWKRAKQLFDEA
                     AAADPHAVVVYDVPLLAEASADRPMRFDLIVVVHANRDARIKRMIELRGMTREEATHR
                     LNSQASDTERLAIADVVIDSNGTLEETLHQADELWEMAAASAVSAQPD"
     gene            2120..>4190
                     /gene="uvrB"
                     /locus_tag="PLW15_09735"
     CDS             2120..>4190
                     /gene="uvrB"
                     /locus_tag="PLW15_09735"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT34712.1"
                     /translation="MQPTRSVRPFEVVSEYVPSGDQPQAIAELAHRINAGETDVVLLG
                     ATGTGKSATTAWLIEQVQRPTLVLSHNKTLAAQLANEFRDLMPNNAVEYFVSYYDYYQ
                     PEAYIPQTDTFIEKDSSINAEVERLRHSTTNSLLSRRDVVVVSTVSCIYGLGAADSYL
                     DAMMAVQVGMRIDRETLIRKFVSMQYQRNDYDFSRGTFRVRGDTIEIIPVYEELAIRI
                     EMFGDEVEAIYALHPLTGEVVRAMDAVSVFPGTHYAASPQTIQRAIGTIQEELAERLQ
                     EFERQGKLLEAQRLRMRTTFDIEMMEQIGFCNGIENYSRHMDGRAPGEAPHCLLDYFP
                     EDFLVVIDESHVTVPQIGAMYEGDASRKRTLVEHGFRLPSAMDNRPLKWEEFLQRVGQ
                     KVYLSATPGKYELGITDSIVEQVIRPTGLVDPQIVVKPSKGQIDDLLEQIRLRVDRDE
                     RVLVTTLTKKMAEELTEFLGEHGVRVRYLHSDVDTLRRVELLTELRQGVYDVLVGINL
                     LREGLDLPEVSLVAILDADKEGFLRSGTSLIQTIGRAARNVSGEVHMYADVMTDSMAK
                     AIEETTRRREKQVAYNEERGIDPQPLRKKIADITDQIMRESSDTAAVLAGKGDASRKR
                     APTPNLKREGLAAQGGNNLEAIIADLNEQMLQAAAELKFELAARLRDEVSDLKKELRQ
                     MEQAGHLR"
BASE COUNT          792 a         1367 c         1376 g          655 t
ORIGIN      
        1 acgtctccga gctgtcgtgg aagcacatcg agcacgcctc cgaggtcgtc gaggttggcc
       61 aggaggtcac cgtggagatc ctcgaggtcg acctcgagcg cgagcgcgtg tcgctgtcgc
      121 tcaaggccac ccaggaggac ccgtggcagg tcttcgcccg cactcacgcg atcggtcagg
      181 ttgctcccgg aaaggtcacg aagctcgttc cgttcggtgc gttcgttcgc gtcgccgagg
      241 gcatcgaggg tctcgtgcac atctccgagc tgtccggcaa gcacgtcgag ctcgctgagc
      301 aggtcgtgtc ggtcggcgac gaggtcttcg tcaaggtcat cgacatcgac ctcgagcgcc
      361 gccgcatctc gctctcgctg aagcaggcga acgagggcgt cgaccccgag ggcaccgagt
      421 tcgacccggc actctacgga atgctcaccg agtacgacga ccagggcaac tacaagtacc
      481 cggacggctt cgacccggag acccaggagt ggaaggaagg cttcgaggcc cagcgcgaga
      541 agtgggagca ggagtacgcc gctgctcagg ctcgctggga gcagcacaag aagcaggtcg
      601 ctgctgcagc cctcgaggag gaccacatca gcgatgtgcc ggccggcgca tccacgttct
      661 ccagcgatga ggtctcgacc ggtggcggaa ctctcgccga cgacgagact cttgccgcgc
      721 tgcgtgagaa gctctcgaac aactcgtagg gtcgtttcga caccgcgtga ttcgacacga
      781 acggccggtt cccctcgggg gccggccgtt cggcgttaga ggggtgaggt gtcctgcact
      841 cgctcgcgct tgcggcggcg accccgacgc acagcggcga cgagcgccac caccagtcca
      901 ccgatggcta ccagcaccag cagcgggaag agcagagcca cgaagctcag gatgacgctg
      961 ccgaagtcct cagcggtgct caccgccggg gcggccgtcc ccgcggtcgc aacgttcagc
     1021 accgggcgag cggccatctt ggtgaggtgg gtgcccagcg cgagcacgac cccgatggcg
     1081 atgggtaccc atgaccccga cgtgaagaag gccgcgggat cggtcaccac tgccgtgccg
     1141 gacgccgacc ctgtaccaaa ggcgattccg cccgcgacgg gccgcacgac tgtctggaaa
     1201 aggtcgttga tggagtcgac cgcgggaatc ttatcggcga cgatctccac gacgaggagt
     1261 actccgagga ttccgaggac ccagggattc tcgagccagg cccagttggc cggtagatcg
     1321 acgaaatcca ggaatcgacc cgcgagtccg agaatgagaa gtggaatgta ggcgttgagg
     1381 cctgccgcga cggcgagacc ggttcccgtc agaaactcca tcgacgaatc ctctcgatct
     1441 agtgccgagg ctagcattgc tgcatgtacc tgatcgggct gaccggcgga attgcctcgg
     1501 gcaagtcggt cgttgcgaag cggctcgccg agcacggcgc agttcacgtg gatgccgatg
     1561 cgctcgcgcg cgaggtcgtc gaacccggca cagaaggcct cgctgcaatc gagcgcgcgt
     1621 tcggaccgca ggtcatcgca cccgacggat cgctcgatcg cccggcgctg ggcgcgatca
     1681 tcttcccgga cgccgagaag cgtgcgctac tgaactccat cacccatccg gcggtgtgga
     1741 agcgcgcgaa gcaactgttc gacgaggcgg ccgcggcgga tccccacgct gtcgtcgtgt
     1801 atgacgtgcc gctgctggcc gaggcatccg ccgatcgacc gatgcggttc gacctcatcg
     1861 tcgtcgtgca cgccaaccgg gatgcgcgca tcaagcgcat gatcgagctg cgcggcatga
     1921 ctcgcgagga ggccacccat cgtctcaact cgcaggccag tgacaccgag cgcctcgcaa
     1981 tagccgacgt ggtcatcgac agcaacggca ccctcgagga gaccctgcat caggctgacg
     2041 agctgtggga gatggcagct gcctccgctg tgagcgcaca accggactag ttgtcagtag
     2101 gcgtgcctaa gctggatgca tgcagcccac gcgatcggtc cgacccttcg aggtcgtgag
     2161 cgagtatgtc cccagcggtg accagccgca ggcgatcgcc gagttggcgc accgcatcaa
     2221 tgccggcgag accgatgttg ttctgctcgg ggcgaccgga acgggcaagt cggcgaccac
     2281 cgcctggttg atcgagcagg tgcaacggcc gaccctcgtt ctgtcgcaca acaagactct
     2341 cgcggcacag ctcgcgaacg aattccgcga tctgatgccc aacaatgcgg tcgagtactt
     2401 cgtctcgtac tacgactact accagcccga ggcgtacatt ccccagacag acaccttcat
     2461 cgagaaggac tcctcgatca acgccgaggt ggagcgcttg cgccattcga ccaccaattc
     2521 gctcctgagc cgacgcgacg tcgtggtggt gtcgacggtg tcgtgcatct acggcctcgg
     2581 cgctgccgac tcgtacctcg acgcgatgat ggcggtgcag gtgggcatgc gcatcgaccg
     2641 tgagacgctc atccgcaagt tcgtgtcgat gcagtaccag cgcaacgact acgacttctc
     2701 gcgcggcacc ttccgtgtgc gcggcgacac gatcgagatc atcccggtgt acgaggagct
     2761 cgcgatccgc atcgagatgt tcggtgacga ggtcgaggcc atctacgctc tgcaccctct
     2821 gacgggcgag gtcgtgcggg cgatggacgc cgtctcggtg tttccgggca ctcactacgc
     2881 tgcgagcccg cagaccatcc agcgcgcgat cggcacgatc caggaggagc tagccgagcg
     2941 cctgcaggag ttcgagcgtc agggcaagct gctggaggcg cagcgcctgc gcatgcgcac
     3001 taccttcgac atcgagatga tggagcagat cggcttctgc aacggcatcg agaactactc
     3061 gcgccacatg gacggtcggg cgccggggga ggctccgcac tgtctgctcg actacttccc
     3121 cgaggacttc ctcgtggtca tcgacgagtc gcacgtgacc gtcccgcaga tcggcgcgat
     3181 gtacgagggg gatgcctcgc gcaagcgcac gctcgtcgag cacggtttcc gcctgccgag
     3241 cgccatggac aaccggccgc tcaagtggga ggagttcctg cagcgcgtcg gtcagaaggt
     3301 ctacctgtcg gccacgcccg gtaagtacga actcggcatc accgactcga tcgtcgagca
     3361 ggtcatccgc cccacggggc tcgtcgaccc acagatcgtc gtgaagccgt ccaagggcca
     3421 gatcgacgac ctgctggagc agatccgact ccgagtcgac cgcgacgagc gcgtgcttgt
     3481 cacgacactc acgaagaaga tggccgagga gctcaccgaa ttcctcgggg agcacggcgt
     3541 gcgcgtgcgg tatctgcact cagacgtcga cacgctgcgc cgcgtcgaac tgctgaccga
     3601 acttcgccag ggcgtgtacg acgtgctggt gggcatcaac ctccttcgcg agggcctcga
     3661 cctgcccgag gtgtcgctcg tggcgatcct cgacgccgac aaggagggct tcctgcgttc
     3721 gggcacctcg ctcatccaga ccatcggtcg agccgctcgc aacgtgtcgg gtgaagtgca
     3781 catgtacgcc gacgtgatga cggattcgat ggcgaaggcc atcgaggaga cgactcgccg
     3841 ccgcgagaag caggtcgcct acaacgagga gcggggcatc gatccgcagc cgctgcgcaa
     3901 gaagatcgcc gacatcaccg accagatcat gcgggagtcg agcgacaccg cggcagtgct
     3961 tgccggcaag ggcgacgcga gccgcaagcg cgcacccacg cccaatctca agcgcgaggg
     4021 cctcgcggcc cagggcggca acaacctcga ggcgatcatc gccgacctca acgagcagat
     4081 gctgcaggcc gctgccgagc tcaagttcga gctcgcggcg cggctgcgcg acgaagtgtc
     4141 cgacctcaag aaggagctgc ggcagatgga gcaggcgggg cacctgcgct
//
LOCUS       DAOSOU010000197         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21873_length_4103_cov_5.364130, whole genome shotgun sequence.
ACCESSION   DAOSOU010000197 DAOSOU010000000
VERSION     DAOSOU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21873_length_4103_cov_5.364130"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLW15_09740"
     CDS             complement(<1..510)
                     /locus_tag="PLW15_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34713.1"
                     /translation="MPVAEDLVAPARERVLNPLTDGLGRSARAHEHRLLSAARPALPV
                     LNWLGMFARPLLRVPQLGWITADPLTARTVLNDPDHFTLLSEGGVGHLWAQILGDWVY
                     DLFDGPGHYDLRTRSRELFTEKTSSGFVQKAWAPRLAEAKAVLDAGGELDLADLSRVL
                     VGQMVVTLLG"
     gene            538..1200
                     /locus_tag="PLW15_09745"
     CDS             538..1200
                     /locus_tag="PLW15_09745"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HOT34714.1"
                     /translation="MEDVRAKYRRVFVDGSNTERLVFFSDAIFAIAMTLLVIDLKVPQ
                     GSTAWEVLVDQSQAFFAYALSFTIIALNWMAHHRKFRVIKAHDGRLMMLDLVLLFFVA
                     FVPFPTSLLSEYGAQPAAVVLYAFVVGLLSILQYLVWSYAWRHGFVDKSVDLELYRFN
                     RRNQFTTPLVFWLSIPIALFIDAQAAMYFWFALFFVNIGLRIYDRARVSRSRDSVSEA
                     AS"
     gene            complement(1160..1651)
                     /locus_tag="PLW15_09750"
     CDS             complement(1160..1651)
                     /locus_tag="PLW15_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT34715.1"
                     /translation="MPTSDEVDRIVDAWSRERPDLDFSPLQVLSRVGRLARHLERARR
                     TAFAASDLELWEFDVLSALRRAGKPYQLSPKALLQQTLVSSGTMTNRIDRLVTRKLVE
                     RRTDPNDGRGILVVMTPEGRERVDAAIRTLLAAESELLDRLSAPDQERLSSLLRKLSL
                     DFD"
     gene            1701..1772
                     /locus_tag="PLW15_09755"
     tRNA            1701..1772
                     /locus_tag="PLW15_09755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1733..1735,aa:Gln,seq:ttg)
     gene            1883..2344
                     /locus_tag="PLW15_09760"
     CDS             1883..2344
                     /locus_tag="PLW15_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34716.1"
                     /translation="MTARPSLVFVCFPAAVRSRLPLWSGHAGRVISAAGRTYSARSPG
                     ADDAWVWSLVSGNNRQLARGASVHPTLAAARRDADRMLQSREILAVELVTGTERARYG
                     WFATVAGHPVATCARWYPTDRDRNHSLDLALNSMGTVAHNDLWRVIALTER"
     gene            2365..2793
                     /locus_tag="PLW15_09765"
     CDS             2365..2793
                     /locus_tag="PLW15_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOT34717.1"
                     /translation="MTKVAFFETVTSIFEIVGVAAMVLGFVGAIVLGIRSLVVTRDGR
                     VAFKTLRDSIGLVILLGLEILVAADLVRTVTSTPSLTDAIVLAIIVLIRTVLSFSLQI
                     EIEGVAPWRRALMTAPEQMARSRQARPPVVERSRDPKPGE"
     gene            2798..>4103
                     /gene="glmU"
                     /locus_tag="PLW15_09770"
     CDS             2798..>4103
                     /gene="glmU"
                     /locus_tag="PLW15_09770"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOT34718.1"
                     /translation="MTDLTLAVVVLAAGQGTRMKSSRPKVLHSIAGRSLIGHVLATAG
                     ELQPGHVIAVVRHARDSVVESITDAMPNVLIVDQDEIPGTGRAVEVAIAALPADFAGD
                     VVVVSGDVPLLDAATLRSLIDTHRAASAAATVLSAILDDATGYGRIVRGSGGEVDRIV
                     EQADATDDERAITEINSGTYVFSVAPLREQLALVGTKNAQGEKYLTDVVGLLRAAGSA
                     VAAMPVPDGWRVSGVNDRVQLAEASRRMNELIVKRWQLAGVTIDDPATTWIDADVTLA
                     EDVQILPGTQLKGATLVQTGATIGPDTTLVDTEVGAGATVKRTDATLAVIGENASVGP
                     FAYLRPGTYLGADGKIGTFVETKNSTIGAGSKVPHLSYLGDATVGEGSNIGAGTITAN
                     YDGVRKNPTIVGDQARTGSHNVFVAPVTIGDGAYTGAGTVVRK"
BASE COUNT          626 a         1389 c         1384 g          704 t
ORIGIN      
        1 cccgagcagg gtcaccacca tctggcccac gagcacccgg gacaggtcgg cgagatcgag
       61 ctcgcctccc gcgtcgagca cggccttcgc ctccgcgagg cgcggcgccc acgccttctg
      121 cacgaagccg gagctggtct tctccgtgaa cagctcgcgg ctgcgggtgc gcaggtcgta
      181 gtggccgggg ccgtcgaaca ggtcgtagac ccaatccccg agaatctgag cccacaggtg
      241 cccgacgccc ccctcgctca gcagggtgaa gtggtcgggg tcgttgagca cggttcgcgc
      301 ggtgagcggg tcggcggtga tccagccgag ctggggcacg cgcagcaggg ggcgggcgaa
      361 catcccgagc cagttgagca ccggcagcgc ggggcgcgcc gcgctcagca gccggtgctc
      421 gtgcgctcgc gcgctgcggc ccaggccgtc agtcagcggg ttgagcacgc gttcgcgggc
      481 gggggcgacg agatcctctg cgacgggcat ggcaccaatc tagctagggt ggtcgccatg
      541 gaggacgtgc gagcgaagta ccgacgcgtg ttcgtcgatg gcagcaacac cgaacggctg
      601 gtgttcttca gcgatgccat cttcgccatc gccatgaccc tgctggtgat cgacctgaag
      661 gtgccccagg gcagcacggc gtgggaggtg ctcgtcgacc agtcccaggc cttcttcgcc
      721 tacgccctct cgttcacgat catcgccctc aactggatgg cgcaccaccg caagttccgt
      781 gtgatcaagg cgcacgacgg ccgcctcatg atgctcgacc tcgtgctgct gttcttcgtc
      841 gccttcgtgc cgttccccac gagtctgctc agcgagtacg gtgcgcaacc ggcggccgtc
      901 gtgctctacg cgttcgtggt ggggctgctg agcatcctgc agtacctggt ctggtcctat
      961 gcgtggcggc acggattcgt cgacaagtca gtcgacctcg agctctaccg cttcaatcga
     1021 cgtaaccagt tcacgacgcc cctcgtcttc tggctctcga tacccatcgc gctgttcatc
     1081 gacgcgcagg cggccatgta cttctggttc gcgctcttct tcgtgaacat cggcctgcgc
     1141 atctacgacc gggctcgcgt cagtcgaagt cgagactcag tttccgaagc agcgagctga
     1201 gtcgttcctg atcgggagca ctgaggcgat cgagcagctc ggactcggcg gccagcagcg
     1261 tgcggatcgc ggcatccacc cgttcgcgcc cctcgggcgt catcaccacg aggatgccgc
     1321 ggccatcgtt ggggtcggtg cgtcgctcca cgagcttgcg ggtgacgagt cggtcgatgc
     1381 ggttcgtcat cgtgccgctc gagacgagtg tctgctgcag cagcgccttc gggctcagct
     1441 ggtagggctt gccggcgcgg cgcagggccg agagcacgtc gaactcccag agctccagat
     1501 cggatgccgc gaacgccgtg cgacgcgccc gctcgaggtg acgcgccagc ctgccgaccc
     1561 ggctgagcac ctgcaggggc gagaagtcga ggtcgggacg ctcccgcgac cacgcgtcga
     1621 cgatgcggtc gacttcgtcg ctggtgggca tccgtcaatt atcgctgtct ggcagacttg
     1681 acgtgcgccg tgaggcgcga tccgccttgg tgtaatggca gcacgacagc ctttggagct
     1741 gttaggtcta ggttcgagtc ctggaggcgg agcattgctg ttcaccgatc ggtgtgctgc
     1801 tgttcgccgg tggttcgcgt tgacgcggtt gagtggtctc atcgcgcggc aggggatcgc
     1861 gccggtgacc gaggtgggcg caatgactgc tcgaccgagc ctggtcttcg tctgcttccc
     1921 tgccgccgtg cgctcgagac ttccgctgtg gagcggtcat gcgggccgtg tgatctccgc
     1981 ggccggccgc acctactcgg ctcgcagccc cggggcggat gacgcgtggg tctggtcact
     2041 ggtgtccggc aacaaccggc agctcgcgcg cggtgcgtcg gtgcacccga ctctggcggc
     2101 agccaggcgc gatgccgatc ggatgctgca gtcccgggag atcctcgctg tggagttggt
     2161 caccggcacc gagcgcgctc gatacggatg gtttgccacc gtcgccgggc atcccgttgc
     2221 aacctgtgcg cgctggtatc ccactgaccg ggaccggaac cattcgctcg atctcgcgct
     2281 gaactcgatg ggaactgttg cgcacaacga tctgtggcga gtgatcgctc tgaccgaacg
     2341 gtaggcgtcg ggtaacctcg cagcatgacc aaggtcgcgt tcttcgagac cgtcaccagc
     2401 atcttcgaga tcgtcggcgt cgccgccatg gttctcgggt tcgtcggggc gatcgtgctc
     2461 ggcatccgct cgctcgtcgt cacgcgtgac ggtcgggtcg cgttcaagac cctgcgcgat
     2521 tcgatcggcc tggtgatcct gctcgggctg gagattctgg tcgccgccga cctggtgcgc
     2581 accgtgacat ccacaccgtc gctgaccgac gcgatcgtgc tcgcgatcat cgtgctcatc
     2641 cgcaccgtgc tgagcttctc gctgcagatc gagatcgagg gtgttgcgcc gtggcgccgc
     2701 gctctgatga cggcacccga gcagatggcc aggtctcgac aggctcgacc accggtggtc
     2761 gagcgaagtc gagacccgaa accgggagaa tagctgaatg accgacctca ctcttgccgt
     2821 ggtcgtgctc gccgctgggc agggcacgcg catgaagtcc tcccgcccga aggtgctgca
     2881 ctcgatcgcc ggccgcagcc tcatcggcca cgttctcgcc accgccggtg agctgcagcc
     2941 cggtcacgtc atcgcggtcg tgcgtcacgc ccgcgactcc gtcgtggagt ccatcaccga
     3001 tgccatgccg aacgtcctca tcgtcgacca ggacgagatc cccggcaccg gtcgggccgt
     3061 cgaggttgcc atagcggcgc tgcccgcaga cttcgctggc gacgtcgtcg tcgtctcggg
     3121 cgacgtaccg cttctggatg ccgcgaccct ccgctccctc atcgacaccc accgcgcggc
     3181 ttccgccgcc gcgaccgtgc tctccgcgat cctcgacgac gccacgggat acgggcgcat
     3241 cgtgcggggc agcgggggag aggtcgatcg catcgtcgaa caggccgacg ccaccgacga
     3301 cgagcgggcc atcaccgaga tcaactcggg aacgtacgtg ttcagcgttg cgccgctgcg
     3361 cgagcagctc gcgctggtcg gcaccaagaa cgcgcagggc gagaagtacc tgaccgacgt
     3421 tgtcggcctg ctgcgggcgg caggctccgc ggtcgcggcc atgcccgtgc ccgacggctg
     3481 gcgggtgtcg ggcgtcaacg atcgcgtgca gttggccgag gcatcccgtc gcatgaatga
     3541 gctgatcgtc aagcgctggc agctcgctgg cgtcacgatc gacgacccgg cgacgacgtg
     3601 gatcgacgcc gacgtcacgc tcgccgagga cgtgcagatt ctgccgggca cccagctcaa
     3661 gggggcgacc ctcgtgcaga ccggtgcgac gatcggcccc gacacgaccc tcgtcgacac
     3721 cgaggtcggt gccggtgcca ccgtcaagcg cacggatgcc acactcgccg tcatcggcga
     3781 gaacgcgagc gtgggcccgt tcgcctacct ccgccccggc acgtatctcg gcgccgacgg
     3841 caagatcggc accttcgtgg agacgaagaa ctcgacgatc ggggcgggca gcaaggtacc
     3901 gcacctcagc tacctcggcg acgcgaccgt cggggagggt tcgaacatcg gagccggcac
     3961 catcacggcc aactacgacg gcgtcagaaa gaaccccacg atcgtgggcg accaggcccg
     4021 caccggctcg cacaacgtgt tcgtcgcgcc cgtgacgatc ggcgacggcg cgtacaccgg
     4081 tgccggcacg gtcgtgcgca agg
//
LOCUS       DAOSOU010000198         4092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21957_length_4092_cov_7.417885, whole genome shotgun sequence.
ACCESSION   DAOSOU010000198 DAOSOU010000000
VERSION     DAOSOU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21957_length_4092_cov_7.417885"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PLW15_09775"
     CDS             complement(<1..976)
                     /locus_tag="PLW15_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT34719.1"
                     /translation="MDAEASQTRGRPNLAAVAALAGVSASTASLAFSGAGPVSEATRA
                     KVFAAAKKLDYAGPDPRAQSLRRGRSGVVGVVIEDRLRDAFRDPINVAMLDGIADALG
                     AESLGLLLLTDTGEGGADISLAPMDGVILIGCSTELDRPVRAFRQRGIPIVAIEAEPM
                     EGVLAIDLDNRDATAHGARHLQNLGHTAVGLVTLPLEGSRQRGELEPHRIAASRAHTT
                     LERIRGARDVFPDASGVVAGGSSVEDGLVAGRDLLSRSPRPTAIIAQSDLLALGVIRA
                     AEELGLTVPDDLSVLGFDGVRIEGSAPHELTTLVQPAVDKGRAAGHAVI"
     gene            1059..2333
                     /locus_tag="PLW15_09780"
     CDS             1059..2333
                     /locus_tag="PLW15_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34720.1"
                     /translation="MSKTSPAPTSYPGGLTRRQVVSWRNAVFLIFAMPGVAIASWVSR
                     LPAVRDALEIGPAQVGLLIFGMAAGAILGLVVSSHLIARIGARTAITAGLTATAVGLA
                     IAGVGGTTPNFIIAFGGLLIFGAASGITDVAMNVSGAANERALQRSIMPIFHAFFSFG
                     TMVGAGLGALAELAGLPIVIHLALVAVAIVAVAFGATRFVQPETVIEANGEPVASDDS
                     SMGWRGRLGIWKDPRTLLIGVIVLAAAFAEGSANDWLAIAMVDGHDVSNANGALVFGI
                     FVTAMTVGRLAGVFLLDRFGRVAVLRASFLLASIGLVIVIFVPIPLIAVAGAVLWGLG
                     AALGFPVGMSAAADDPRTAAARVSAVATIGYFAFLVGPPVIGLLGQYVGVLDALLVVL
                     VLCAIGAVVAGAAREPSREASAPVDTFSDPQS"
     gene            2352..3047
                     /gene="nucS"
                     /locus_tag="PLW15_09785"
     CDS             2352..3047
                     /gene="nucS"
                     /locus_tag="PLW15_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOT34721.1"
                     /translation="MRLVVAKCSVDYAGRLSAHLPLATRLLLVKADGSVLVHSDSLSY
                     KPLNWMSPPCTIAVIEPEAEQAEAGVIEVWKVAQAKTADLLVISIHEILHDSAHELGH
                     DPGLQKDGVEAHLQKLLAEQIELLGEGTRLVRREYMTAIGPVDILATDAAGGSIAVEI
                     KRRGDIDGVEQLTRYLELMNRDPLLAPVAGVFAAQEIKPQARTLAQDRGIRCLVLDYD
                     AMRGLDDSHSRLF"
     gene            3052..3711
                     /locus_tag="PLW15_09790"
     CDS             3052..3711
                     /locus_tag="PLW15_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT34722.1"
                     /translation="MSAPDWTCILFDLDGTIADSAPGITASLGYMFEELGLPVPSPEE
                     LREWVGPPILDSFRDRAGMTPTQAAQALAIYRDHYLEHGGTGAPIFEGIPHVLRTIHQ
                     SGTPLALATSKPEFPASIILDHGNLTANFRVISGSSIDEIRSAKKDVIAEALTRFRMI
                     GVDTSRPVMVGDRDYDVEGAAANGMPAIFVTWGYGKPGEEQGSIAQVGHPRELLPLLL
                     G"
     gene            complement(3724..3930)
                     /locus_tag="PLW15_09795"
     CDS             complement(3724..3930)
                     /locus_tag="PLW15_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34723.1"
                     /translation="MSDVKVPWWDRLNDALLPYIGPPPLGPYDEEPLPPTGAKACPLC
                     GKPMEEHTFERSQDRHSTRMHCPR"
     gene            3953..>4092
                     /locus_tag="PLW15_09800"
     CDS             3953..>4092
                     /locus_tag="PLW15_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT34724.1"
                     /translation="MAAVDPLALDRQVCFALAAASRSVIGLYRPVLEPLGLTHPQYLV
                     MLA"
BASE COUNT          572 a         1476 c         1367 g          677 t
ORIGIN      
        1 cgatgacggc gtgccccgcc gcccggccct tgtcgaccgc cggctgcacg agcgtcgtca
       61 gctcgtgcgg tgctgagccc tcgatgcgca ccccgtcgaa gcccagcaca ctgagatcgt
      121 cgggcacggt gagacccagc tcctcggcgg cacggatgac gcccagcgcg agcaggtcgc
      181 tctgcgcgat gatcgccgtc gggcgcggac tgcgcgagag gaggtcacgc cccgcaacca
      241 ggccgtcctc cacactgctg ccgcccgcga caacaccgct cgcgtcgggg aacacatcgc
      301 gggcaccccg gatgcgctcc agggtggtgt gcgcccggct ggccgcgatg cggtgcggtt
      361 cgagctcgcc gcgctgcctc gatccctcga gcggcagggt gacgagcccc acggcggtgt
      421 ggccgaggtt ctggaggtgc cgggctccgt gcgccgtggc atcccggttg tcgagatcga
      481 tcgccagtac cccctccatc ggctcggcct cgatggcgac gatcgggatg ccccgctgac
      541 ggaacgcccg gaccggcctg tcgagctccg tgctgcaccc gatgaggatg acgccgtcca
      601 tcggcgcaag cgagatgtcg gctccaccct cgccggtgtc cgtgagcagc agcaggccga
      661 ggctctcggc gccgagggca tcggcgattc cgtcgagcat ggccacgttg atggggtcgc
      721 ggaatgcgtc ccgaagccgg tcctcgatga cgacgccgac gactcccgac cggccgcgtc
      781 gtagcgactg ggctcggggg tcgggcccgg catagtcgag cttcttcgcg gccgcgaaca
      841 ccttggcgcg ggttgcctct gacaccgggc cggcgccgct gaaggcgagc gacgccgtcg
      901 acgccgagac gccggcgagt gccgcgacgg cggcgaggtt gggtcgcccc ctggtctggc
      961 ttgcctcggc gtccatgggt tggtagctta gtcgaatcga atcgatcgaa tcgattcgac
     1021 ctctgtcgcc gaaccggccc cgagctcgca aggaacccat gtcgaagacg tcgcctgccc
     1081 ccaccagcta ccccggtgga ctgacccgtc gccaggtcgt ctcgtggcgc aacgccgtct
     1141 tcctgatctt cgccatgccc ggtgtcgcca tcgccagctg ggtctcccga cttcccgccg
     1201 tgcgcgacgc cctcgagatc gggcccgctc aggtcgggct gctcatcttc ggcatggcgg
     1261 ccggcgcgat cctgggtctc gtcgtctcca gccacctcat cgcgcgcatc ggcgcgcgca
     1321 cggcgatcac ggccggcctc accgcgacgg ccgtcggcct cgcgattgcg ggcgtcggtg
     1381 gaacgacacc caacttcatc atcgcgttcg gcggtctgct gatcttcggt gcggcatccg
     1441 gaatcaccga cgtcgccatg aacgtctcgg gggccgcaaa cgagcgcgcc ctgcagcggt
     1501 cgatcatgcc catcttccac gccttcttca gcttcggaac catggtgggc gccgggctcg
     1561 gagcgctcgc cgaactcgcc ggcctgccga tcgtcatcca cctcgcgctc gtcgccgtgg
     1621 ccatcgtcgc tgtcgcgttc ggtgccaccc gcttcgtgca gcccgagacc gtcatcgagg
     1681 ccaacggcga acccgtcgcc tcggatgatt cgtcgatggg ctggcgcgga cgactcggca
     1741 tctggaagga cccgcgcacc ctcctgatcg gcgtgatcgt gctggccgcc gcattcgccg
     1801 aaggctcggc caacgactgg ttggcgatcg ccatggtcga cggccacgac gtgagcaacg
     1861 cgaacggcgc gctcgtattc ggcatcttcg tcaccgcgat gaccgtcggc cggcttgccg
     1921 gggtgtttct gctcgaccga ttcggccggg tcgcggtgct gcgggcatcg ttcctgttgg
     1981 catcgatcgg actggtgatc gtcatcttcg ttccgatccc gctgatcgct gtcgcgggcg
     2041 ccgtgctctg gggcctcggg gctgcgctcg gcttccccgt gggcatgtcg gccgccgccg
     2101 acgatccgcg aacggctgcg gcccgcgtga gtgccgtcgc cacgatcggc tacttcgcgt
     2161 tcctcgtggg tcccccggtc atcgggctcc tgggccagta cgtcggcgtg ctcgacgcgc
     2221 tgctcgtggt gctcgtgctc tgcgcgatcg gtgcggtcgt cgcgggtgcg gcccgcgaac
     2281 cgtctcgcga ggcatccgct cccgtcgaca ccttcagcga ccctcagtcg tagtcctcct
     2341 aggctggacc catgcgtctg gtggtagcga agtgctcggt cgactacgcc ggacggttga
     2401 gcgcgcacct gccgctggcc acccgcctgc tgctcgtcaa ggccgacggc agcgtgctgg
     2461 tgcacagcga ctcgctgagc tacaagccgc tgaactggat gagcccgccc tgcacgatcg
     2521 ccgtcatcga gcctgaggcc gagcaggccg aggcgggcgt catcgaggtc tggaaggtcg
     2581 cccaggcaaa gaccgccgac ctgctcgtga tctcgatcca cgagatcctc cacgacagcg
     2641 cccacgagct cggccacgac cccgggctgc agaaggacgg cgtcgaggcc cacctgcaga
     2701 agctgctcgc cgagcagatc gaactgctcg gtgagggcac ccggctggtg cgtcgcgagt
     2761 acatgacggc catcggcccg gtcgacatcc tggcgacgga tgccgcgggc ggcagcatcg
     2821 ccgtcgagat caagcggcgc ggcgacatcg acggcgtcga gcagctcacc cgctacctcg
     2881 aactcatgaa tcgcgacccg ttgctcgcgc cggtggcggg agtcttcgcc gcccaggaga
     2941 tcaaaccgca ggcccgcacg ctggcccagg atcgcggcat ccgctgtctg gtgctcgact
     3001 acgacgccat gcgcggactc gacgactcgc acagccgcct attctgattc agtgagcgct
     3061 cccgattgga cctgcatcct gttcgacctg gatggcacga tcgcggactc cgctcccggc
     3121 atcacggcgt cgctcgggta catgttcgag gagttggggc ttccggtgcc ctcccccgag
     3181 gagctgcggg agtgggtcgg cccgccgatc ctcgattcgt tccgcgaccg ggcggggatg
     3241 acgcccacgc aggccgccca ggcgctggcc atctaccgcg accactacct cgagcacggc
     3301 ggcaccggtg ccccgatctt cgaggggatc ccgcacgtgc tgcgcaccat ccaccagtcg
     3361 ggaacaccgc tcgcgctggc cacgtcgaag cccgagttcc ccgcgtcgat catcctcgac
     3421 cacggcaacc tgaccgcgaa cttcagggtg atctccggtt cgtcgatcga tgagatccgc
     3481 agcgccaaga aggacgtcat cgccgaggcg ttgacccgct tccgcatgat cggggtcgac
     3541 accagtcgcc ccgtgatggt gggggaccgc gactacgacg tcgagggtgc cgcggccaac
     3601 ggcatgcccg cgatcttcgt cacctggggt tatggcaagc cgggcgagga gcagggctcc
     3661 atcgcccagg tcggccaccc gcgcgagctg cttccgttgc tgctgggctg agcccccggc
     3721 ccgtcagcgc gggcagtgca tccgggtgga gtgacggtcc tggctgcgct cgaaggtgtg
     3781 ctcctccatc ggcttgccgc agagcgggca cgccttcgcg ccggtcggcg gcagcggctc
     3841 ctcgtcgtag gggccgagcg gcggcggccc gatgtagggc aggagggcgt cgttcagacg
     3901 atcccaccac ggcactttta catcgctcac aaacagttag tatactaacg aaatggccgc
     3961 cgtcgacccc ctcgcccttg accgccaggt ctgcttcgcc ctcgccgccg cctcgcgcag
     4021 cgtgatcggg ctctaccgac cggtgctgga gccgctcggc ctgacccacc cgcagtacct
     4081 ggtgatgctc gc
//
LOCUS       DAOSOU010000199         4090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_21972_length_4090_cov_3.395043, whole genome shotgun sequence.
ACCESSION   DAOSOU010000199 DAOSOU010000000
VERSION     DAOSOU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21972_length_4090_cov_3.395043"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLW15_09805"
     CDS             <1..491
                     /locus_tag="PLW15_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT34725.1"
                     /translation="RRRVEVSESASEAHGKFEVGATKTYERRNVPVPAALIDQLVHHI
                     AGKGPDDFVFESPDGGPVRHSNWYPRHFKPAVVRAGLPDGTRFHDLRHTYAAMLIAQG
                     AHPRAMMERLGHSTIQVTLGTYGHLFPNLEESLDHALNSVFEGASPTEPAPTLNVRRS
                     AR"
     gene            complement(949..1413)
                     /locus_tag="PLW15_09810"
     CDS             complement(949..1413)
                     /locus_tag="PLW15_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOT34726.1"
                     /translation="MNALFPAAERIARDDGIDQPGAEHLLLAALDLDDGIAKNALNTF
                     QVNPAQLRAAIVGQHEEALRAIGVIADDNAIDAVLPASGQQRGPYRAQGSLQTAFQHA
                     VALAKRDKAPLNSGYLLLAATDADHGTVARSLEHLGVDRASLQDRTRSHLAR"
     gene            complement(1470..1682)
                     /locus_tag="PLW15_09815"
     CDS             complement(1470..1682)
                     /locus_tag="PLW15_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HOT34727.1"
                     /translation="MIEKVPDPSNPVEALAAVVSLRRLADRLEAASVQAAIDQGWNWV
                     QIAEALGVTKQAVHKKYARRVVRRAT"
     gene            1734..2777
                     /locus_tag="PLW15_09820"
     CDS             1734..2777
                     /locus_tag="PLW15_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT34728.1"
                     /translation="MRAIPAHTLALLILGTVIAGCTNDTNDNATPTSSTTDPTGSSAA
                     VDLAVLAPRAGYGIYDTDDGHCTLLAGHGLTDPVPVASMFKLWVLDALARAIANGNIG
                     WDTLVPIRDDLRSDPSGEVYGYPTGQRVTVSQIAELMISISDNTATDHLIELIGRDAI
                     SDVIRELAPDGASRTLPLLSTADMARLKFVHPNLGREYAALDPDARSEFVTTLPERGP
                     FPWPDDPAAINRLDLTTPSFVDEIEWFASGTDICRTMVDLARLSHEAGPEPLDGILSA
                     NPGLPPDQARAWTTTWFKGGSEPGVVALAYRLANNDLDRVVVIALSDPDAPLSQRPGG
                     QEIIETILTAAQG"
BASE COUNT          711 a         1452 c         1252 g          675 t
ORIGIN      
        1 tgcgccggcg cgtggaggtg tcggagtcag cgtccgaggc gcacggcaag ttcgaggtcg
       61 gcgccaccaa gacctacgaa cggcgcaacg tcccggtgcc cgccgctttg atcgaccagc
      121 tcgtccacca catcgccggc aagggaccgg acgacttcgt cttcgagagc cccgacggcg
      181 gtccggtccg tcactcgaac tggtaccccc gccacttcaa gccggccgtc gtacgagcgg
      241 gcctccctga cggcacccgc ttccacgacc tgcgccacac ctacgccgcg atgctcatcg
      301 cccagggagc ccaccccagg gccatgatgg aacgcctcgg ccacagcacg atccaggtca
      361 ccctcggcac ttacggccac ctcttcccga acctcgaaga gagcctcgac cacgccctga
      421 actccgtgtt cgagggcgcc agtcctactg aacctgcacc gacgctgaat gtgcggcgct
      481 ccgctcggta gcagttgatc gagcgaggcc ggagacacgt gccaagcgga catcggctta
      541 cccggactat gcagacgccg ccggattgca gacatgacga gcctgaaagg ccgcggcgcc
      601 gccttcgagg atcttccgtg acaactcggc gacggcgcga tcctcaagcg ccctcaccgc
      661 aacgcgagcc cgaccatgct ccgagtgcgc atcgagcgcg gggtcgagtt cagcgtcctc
      721 gagtatcgag atgctggccc tatcgtgatc ctggcttgtg cgtcgcgacg atcacgtgtc
      781 gtgtctcgca cgccgcggcc agcaccttca cgacttcgtc gtagatgcat cctcaggctg
      841 atcgatcagg agtggctcgc atcgaccatc gagagccaac gccagcaagc cagcgcccgg
      901 gtctcgcgtg tggctcacca cgccggcggg cgagtgagcc cgatgatctc atcgggcgag
      961 gtggctgcgg gtgcgatctt gaagcgaggc tcggtcgacg ccgagatgct cgagcgatcg
     1021 ggcaacggtg ccgtgatcgg cgtcggtggc cgcgaggagc aggtagccgc tgttcaacgg
     1081 tgccttgtca cgcttcgcga gcgcgaccgc gtgttgaaag gcggtctgca acgatccttg
     1141 cgcgcggtac ggcccccgct gctgcccgct ggccggcagg acagcatcga tcgcgttgtc
     1201 gtcggcgatc accccgatcg cgcgcagcgc ctcttcgtgt tgcccgacga tcgcagcccg
     1261 gagctgcgcc gggttgacct ggaaggtatt cagcgcgttc ttcgcaatgc catcgtcgag
     1321 gtcgagcgcc gcaagcagca gatgctcggc gcccggctgg tcgatcccat catcacgtgc
     1381 gatccgctcc gccgccggga acagcgcatt catcgtcgcg agatcggcgc gagctcgacg
     1441 caacttgttg aacatgggct tccttctgat cacgtggccc ggcgcacgac gcgtcgggcg
     1501 tacttcttgt gcactgcctg tttggtgacg ccgagtgcct cggcgatctg gacccagttc
     1561 catccctgat cgatcgcggc ctgcaccgag gcagcttcga gccggtcggc gagccgacgc
     1621 agcgacacca ccgctgcgag agcttcgacc gggttgctcg gatccggcac cttctcgatc
     1681 acgcagtcaa ctttagttga ccgaccgacc ccggtcaact aacattgacc gtcatgcgag
     1741 cgatccccgc acatactctt gctctgttga tcctcggcac ggtcatcgcg gggtgcacga
     1801 acgacacgaa tgacaacgcg actccgacga gctcgaccac cgatccgacc ggctcgtccg
     1861 ccgcggtgga tctcgctgtc ctcgctcccc gcgcgggtta tggcatttac gacaccgacg
     1921 atggacactg cacgttgctc gccgggcatg gcctgacgga tcccgtcccc gtagcgtcga
     1981 tgttcaagtt gtgggtactc gatgccctcg cccgagcgat tgcgaacggc aacatcggct
     2041 gggacaccct cgtgcccatt cgcgatgacc ttcgtagcga tccgtcaggt gaggtgtacg
     2101 gctacccaac tggccagcga gtgaccgtgt cacaaatcgc ggagctgatg atctccatct
     2161 cggacaacac cgcgaccgat cacctcatcg aactcatcgg tcgcgacgcc atcagcgacg
     2221 tgatccgcga actcgccccc gacggggcct cccgcaccct gccactgctc tcgaccgctg
     2281 acatggcccg cctcaagttc gtgcacccga acctcggccg cgagtacgcc gccctcgacc
     2341 cagatgccag atcggagttc gtgacaacgc tccccgaacg cggcccgttt ccgtggcccg
     2401 acgaccccgc ggcgatcaac cgcctcgacc tgaccacgcc cagcttcgtc gacgagattg
     2461 agtggttcgc gagcggcacc gacatctgtc gaacgatggt cgacctcgcg cgtctttccc
     2521 acgaggctgg acctgaacca cttgacggca tcctcagcgc caacccgggg ttgccacctg
     2581 atcaagcgcg agcgtggacc accacatggt tcaaaggcgg ctccgaaccc ggcgtcgtcg
     2641 cgctcgccta ccgacttgca aacaatgatc tcgaccgcgt cgtcgtcatc gccctttccg
     2701 atcccgacgc gccactctca caacggcccg gaggccagga gatcatcgag acgatcctca
     2761 ccgccgccca aggctgagca acaacaacgc cccgatatat ggacgccgag aaggacccga
     2821 acccggctac cgaggcaccg acgccgaccg accgcgtctg gcaacgcctc ctcgcgttac
     2881 ccctcgcccc gatcgtcgct gccatcgacc tcacctccgc catcttcata gcgtgggctg
     2941 aatggggaac ccgacagctc aaccgcctcg acccattctt cgcgaggttc acgaggctga
     3001 tgatgagact cgggaactgg gcagcaccag cggctgcgcg catcagcgcg agcatcgcca
     3061 ggctcaccac cgccctcgcg cccctcatga ccgcggcggg acgtctcgcg accagaatgg
     3121 gcgcgctcgt gcgacgcgtg acccaaccct tgggccacgg gttggtcaca gttcgaagca
     3181 tcgttcgccg agccatcagc cgcatcagca ccccgctgcg acggctcacg tcccgcgtcg
     3241 tctcagcagc ctcacggacg accagccgcg cccgaagcac gatctcagcg ctacgccgag
     3301 ccgctcgtcc acgacgaatg gcagcgcaca cggcatcaac cgcgccaggt gggacggacc
     3361 gctgaccacc ccgacataca aggagccaac gggtgcacgt ccccgcacgg tgcggccaca
     3421 ccgcccggca ggccaagcca ggtgatcgag ggcgcaggtc caacgagcgc cgtcgggcgc
     3481 caacagaccg cgctgacacc atcgctcaac gcaccacctc gcaagatgac cgtcgggttg
     3541 ggtcgcgcag cgcgaactgc tgacatgcac agggctacac agggcgtcgt tggacaccgt
     3601 tgggcgcctg gcgggcgcca tgttgggcgc cgttgggcgc cgtcgtgtgc catttctgtg
     3661 ccatggccga aaacgccgaa ggggcctcgc cggagcaaga ccccttctga gctggcatta
     3721 cctgagtggc gggggcagga tttgaacctg cgaccttcgg gttatgagcg tgcagatcgc
     3781 cgtctgcgcc tggtcggccg gatgggcgtt tgtcctggta gagcagggtt ttcggcttct
     3841 ggcgggtcgt gtcgtagctg cccgtgggag gccgtttgtg ggcgtcgtgc ttacaaattg
     3901 ctgacaagcg ccggccgccc cagtcactgc gtcgccggcg aatcggcgtc ggtctctcag
     3961 gtccacggca aggctcgagg tcaccgccga cgtcgagcgc ctcagcctgc tcgacgggtc
     4021 gccgagtgcg ctcgtcgaca gagccccgcg gggtggtggg aaatcgagtg tgatgctcga
     4081 tgtgttcagc
//
LOCUS       DAOSOU010000200         4017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_22539_length_4017_cov_5.436143, whole genome shotgun sequence.
ACCESSION   DAOSOU010000200 DAOSOU010000000
VERSION     DAOSOU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22539_length_4017_cov_5.436143"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLW15_09825"
     CDS             complement(<1..363)
                     /locus_tag="PLW15_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOT34729.1"
                     /translation="MRSLNVVVGVTGGIAAYKAVSVVRALVLAGHDVHVVATENALRF
                     VGRPTLEAISRNTVHSDLYEGVAEVRHVAIGQAADLIVVAPATANTVAKLAAGLADDL
                     LGNTILASTAPLVIAPAMH"
     gene            complement(393..653)
                     /gene="rpoZ"
                     /locus_tag="PLW15_09830"
     CDS             complement(393..653)
                     /gene="rpoZ"
                     /locus_tag="PLW15_09830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT34730.1"
                     /translation="MVDKHSGIIDPPIDELLSKVDSKYQLVIFASKRARQINDYYADL
                     HEGSLHDNVGPLVDSTIDDKPLSVALHEINDDKLELKRLSSE"
     gene            complement(689..1588)
                     /gene="gmk"
                     /locus_tag="PLW15_09835"
     CDS             complement(689..1588)
                     /gene="gmk"
                     /locus_tag="PLW15_09835"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT34731.1"
                     /translation="MSPRPTPPDVDRAAASRAAIAARRARAEIKRQVASRERAAADVA
                     TVGWVDTDSPEAGMRVRELLLSVPSLGPTRVERVLTQLEISDKKRVGGLGPRQRERLY
                     DYLVARQGGEPARLVVLAGPTAVGKGTVSSYIRDHHPEVSLSVSATTRKPRPGEVDGV
                     HYYFVSDAEFDRMIAAGELLEWAVVHNSHRYGTPRPPIDAAIAEGRRVLLEIDLQGAR
                     QVRAAMPEALLIFLLPPTWEELVRRLTGRGTEDTEEQQRRLETARIELAAQDEFDAKV
                     VNREVSQAAREVVELMDAPFRAP"
     gene            complement(1585..2433)
                     /gene="pyrF"
                     /locus_tag="PLW15_09840"
     CDS             complement(1585..2433)
                     /gene="pyrF"
                     /locus_tag="PLW15_09840"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT34732.1"
                     /translation="MIGFGDRLASTLADRGPLCLGIDPHAFLLADWGLTDDAAGVREF
                     SLRSVDAAAAVIGIVKPQVAFFERHGAAGYAVLEEVLAAARAAGLLVIADVKRGDVGS
                     TVAAYGEAWLTPGSSLEADAMTIVAYQGLGSLDDPVARADANGKGLFVLAATSNPEAA
                     ATQTARVGSGEFAGRSVAAGIVSGVEQLNSSADGVGSVGVVLGATVRLEDYGIDVAQL
                     RKTPILAPGFGEQGARIADLPTLFGSAAGTVIANVGRSVLRAGSAGVRDELISLTHEL
                     REAGSR"
     gene            complement(2430..>4017)
                     /gene="carB"
                     /locus_tag="PLW15_09845"
     CDS             complement(2430..>4017)
                     /gene="carB"
                     /locus_tag="PLW15_09845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOT34733.1"
                     /translation="ASFALSDAGFETIMINCNPETVSTDYDTSDRLYFEPLTLEDVLE
                     IIHAESASGELVGVIVQLGGQTALGLAQGLKDAGVPILGTTPEAIDLAEERGLFSRIL
                     DDAGLLAPKNGTATNRAEAITIAESIGYPVLVRPSHVLGGRGMEIVYDTESLADYFLR
                     VKDQAIIAPGSPLLVDRFLDDAIEIDIDALYDGTELYIGGIMEHIEEAGIHSGDSSCT
                     LPPVTLGRDVINRVREATLAIAAGIGVRGLLNVQFAIGQGILYVLEANPRASRTVPFV
                     SKALGIPLAKAASLVMVGRSIRSLVDSGLLPRQDGSIVPMDAPVSVKEAVLPFKRFRT
                     VEGHVVDSVLGPEMRSTGEVMGIDSNFPKAFAKSQEAAYGGLPASGSVFVSVADRDKR
                     AIVLPVLRLSQLGFTILATEGTAEVLARNGIEAKVVRKYFMGQEVVDGQPSIVELINT
                     GEVDVVINTPSGRSARADGYEIRTATVAADKPLFTTIAQLGAAVASFEAIRDGFDVRS
                     LQDYAIDRADRLARSTAAGA"
BASE COUNT          686 a         1324 c         1346 g          661 t
ORIGIN      
        1 gtgcatggcc ggggcgatga cgagcggggc cgtgctcgcg aggatcgtgt tgccgaggag
       61 gtcgtccgca agcccggccg cgagtttggc tacggtgttc gccgtcgccg gggcgacgac
      121 gatgaggtcg gcagcctggc cgatggcgac gtgccgaacc tcggcgacac cctcgtacag
      181 atcagagtga accgtgttgc ggctgattgc ctcgagagtg ggccgaccga caaaccgaag
      241 tgcgttctcg gtggcgacga catgaacgtc atgtccggcc agcaccagcg ccctgacgac
      301 cgagaccgcc ttgtacgcgg cgatgccgcc ggttacaccg acgacgacat tgagggagcg
      361 caccccgtga accgacacgt tcgaggtggg tgctactccg agctcaggcg cttgagctcg
      421 agcttgtcgt cgttgatctc gtgcagggca accgacagcg gcttgtcatc gatcgtcgag
      481 tcgaccaggg gtccgacgtt gtcgtgcagg ctgccctcgt ggaggtcggc gtagtagtcg
      541 ttgatctggc gcgcacgctt cgacgcgaag atgacgagct ggtacttgga gtcgaccttg
      601 gagagaagtt cgtcgatggg cggatcgatg atgcccgagt gcttgtcaac catggtggtg
      661 ctccttgtgt gaagaaaggg atgggaagtc agggcgcgcg aaaaggcgca tccatcaatt
      721 ctacgacctc acgggccgct tggctgacct cacggttgac gaccttcgcg tcgaactcat
      781 cctgggcggc caattcgatc ctcgccgtct ccagccgacg ctgctgttcc tcggtgtcct
      841 cggtgccgcg gccggtgagc ctgcgcacca gttcctccca ggtcggcggc agtaggaata
      901 tcagcagcgc ctcgggcatg gccgctcgca cctgccgtgc gccctgcagg tcgatctcga
      961 gaagaacgcg tcgaccttcg gcgattgccg cgtcgatggg tggtcggggg gttccgtacc
     1021 ggtgggagtt gtgcaccacg gcccactcga gcaactcccc cgcggcgatc atccgatcga
     1081 actcagcgtc gctcacgaaa tagtagtgaa ctccgtcgac ctcaccaggg cgaggtttgc
     1141 gagttgtggc cgagacactc agcgagacct cggggtgatg atcgcgaatg taggaggaca
     1201 ccgtgccctt gcccacggcc gtcgggccgg cgagcacgac gagtcgcgct ggttcgccgc
     1261 cctgacgagc cacgagatag tcatagagtc tctcgcgctg acgaggaccg agccccccaa
     1321 cgcgcttctt gtccgagatc tcgagctgag tcagaacccg ctcgacccgg gtcgggccga
     1381 ggctcggaac gctgagcagc agctcacgca cccgcattcc ggcttcgggg ctgtcggtat
     1441 caacccagcc gaccgtcgcg acgtcggccg ccgctcgctc gcgggatgcc acctgccgct
     1501 tgatctccgc cctggcgcgt cgcgcggcga ttgccgcccg gctcgccgca gcgcggtcga
     1561 cgtcgggtgg ggtgggacgg gggctcacct cgaccccgcc tctcgtagtt cgtgggtcaa
     1621 cgagatcagt tcgtctcgca ctcccgccga cccggctcgc agaacggagc ggccgacgtt
     1681 ggcgatcacg gtgccggcag ccgagccgaa cagcgtagga aggtcggcaa tgcgagcgcc
     1741 ctgctcaccg aacccgggcg cgagaatcgg cgtcttgcgc aactgtgcga cgtcgatgcc
     1801 gtagtcctca agacgaaccg tggcgccgag cacaacaccg acggatccga caccatctgc
     1861 gctcgagttc agctgctcca cccctgagac gataccggcc gcaaccgacc gacccgcgaa
     1921 ctctcctgaa cccacccgcg ccgtctgggt tgcggccgcc tccggattcg acgtggcggc
     1981 gagaacgaac agccccttgc cgttggcgtc agcgcgcgcc accggatcgt ctagggagcc
     2041 gagcccctga tacgcgacga tcgtcatggc atccgcttcg aggctgctgc ccggggtcag
     2101 ccaggcctcg ccgtaggcag cgacggtgga acccacgtct ccccgcttga cgtcggcgat
     2161 cacgagcaga ccggcagcgc gggctgccgc gagtacctcc tcgagcacgg cgtagcccgc
     2221 cgcaccgtgc cgttcgaaga aggcgacctg cggcttgacg atgccgatga cggcagccgc
     2281 ggcatccacg gatcgcaggg agaattcacg cacgccggcg gcgtcgtcgg tcagccccca
     2341 gtcggcgagg agaaaagcgt gcgggtcgat tcccaggcac aacggtccgc ggtcggccag
     2401 ggtgctggcc agacggtcgc cgaagccgat catgctcccg ctgccgtgga tcgtgccaga
     2461 cgatcggccc ggtcgatcgc gtagtcctgc agcgatcgca cgtcgaaccc gtcgcggatg
     2521 gcctcgaagc tcgcgacagc tgcaccgagc tgggcgatgg tcgtgaagag cggcttgtct
     2581 gcagccacgg ttgcggtgcg gatctcgtag ccgtcggcgc gagcgctgcg gccgcttggg
     2641 gtgttgatga ccacgtcgac ctcgccggta ttgatgagct cgacgatgga cggctgcccg
     2701 tcgacgacct cctggcccat gaagtacttg cgcaccacct tggcctcgat gccgttgcgg
     2761 gcaagaacct ccgccgtacc ctcggtcgcg agaatggtga accccagttg gctcagtcgc
     2821 agcacgggca gcacgatggc gcgcttgtcg cggtcggcga ccgacacgaa caccgatccg
     2881 ctcgcgggca gtccaccgta ggccgcctcc tgcgacttgg cgaacgcctt ggggaagttc
     2941 gagtcgatac ccatcacctc gccggtggag cgcatctccg gcccgagaac cgagtcgacg
     3001 acgtggccct cgacagtgcg gaagcgcttg aatgggagca ccgcctcctt gaccgacacc
     3061 ggcgcatcca tcggcacgat cgagccgtcc tgccggggca gcaaaccact gtcgacgagc
     3121 gagcggatgc tgcgaccgac catcacgagc gacgcggcct tggccagcgg aatgcccagc
     3181 gccttcgaca cgaacggcac agtacgggat gcccgggggt tggcctcgag cacgtacagg
     3241 atgccctgcc cgatcgcgaa ctgcacgttg agaaggcccc gcactccgat gcccgcggcg
     3301 atcgcgagtg tcgcctcacg cacgcgattg atgacgtcgc ggccaagggt gaccggcggg
     3361 agcgtgcacg acgagtcgcc cgaatggatg cccgcctcct cgatgtgctc catgattccg
     3421 ccgatgtaga gctcagtgcc gtcgtagaga gcatcgatgt cgatctcgat ggcgtcgtcg
     3481 aggaagcgat cgacgaggag cggggagccc ggcgcaatga tcgcctgatc cttgacgcgc
     3541 aagaagtagt cggcgagcga ttcggtgtcg tagacgatct ccatgccgcg cccgcccaga
     3601 acgtgactgg gacgcacgag caccgggtaa ccgatggact cggcgatcgt gatcgcctcg
     3661 gccctgttgg tcgccgtacc gttcttcggt gcgagcagac cggcatcgtc gaggatgcgg
     3721 gagaacagtc cgcgctcctc cgcaagatcg atggcttcgg gtgtcgtgcc gaggatcgga
     3781 acaccggcat ctttgagtcc ctgagccagc ccgagagcgg tctggccacc gagctgcacg
     3841 atcaccccca ccagctcgcc cgatgcactc tcggcgtgga tgatctccag aacgtcctcg
     3901 agggtgagcg gctcgaagta cagccggtcg ctggtgtcgt agtcggtcga caccgtctcg
     3961 gggttgcagt tgatcatgat cgtctcgaac ccggcgtcgg agagcgcgaa gctcgcg
//
LOCUS       DAOSOU010000201         4002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_22647_length_4002_cov_6.404104, whole genome shotgun sequence.
ACCESSION   DAOSOU010000201 DAOSOU010000000
VERSION     DAOSOU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22647_length_4002_cov_6.404104"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..552
                     /gene="rplU"
                     /locus_tag="PLW15_09850"
     CDS             244..552
                     /gene="rplU"
                     /locus_tag="PLW15_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT34734.1"
                     /translation="MVYAVVRAGGRQEKVEVGSIVVLDRIQADKNGNVELVPVLFVDG
                     DKITHDAKSLTKVKVVAEVLGNERGPKIVIQKFKNKTGYKKRQGHRQELTRIKITSIA
                     "
     gene            565..819
                     /gene="rpmA"
                     /locus_tag="PLW15_09855"
     CDS             565..819
                     /gene="rpmA"
                     /locus_tag="PLW15_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT34735.1"
                     /translation="MAHKKGASSTRNGRDSNAQRLGVKRFGGQTVLAGEIIVRQRGTH
                     FHPGANVGRGGDDTLFALAAGAVEFGAKGGRKVVNIVTAD"
     gene            940..2466
                     /gene="obgE"
                     /locus_tag="PLW15_09860"
     CDS             940..2466
                     /gene="obgE"
                     /locus_tag="PLW15_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT34736.1"
                     /translation="MASFVDQVTLHLLAGHGGNGCVSVKREKFKPLAGPDGGNGGDGG
                     DIVLVSDPQVTTLLGFHRAPHRKSEHGQPGQGDHKAGTSGEDLILSVPVGTVIKDAEG
                     NELIDMDAPGIRYVAGPGGQGGLGNAALATTKRKAPGFALLGTPGFEGDIFLELKTVA
                     DVALVGYPSAGKSSLVAALSAAKPKIADYPFTTLHPNLGVVESGDTRYTIADVPGLIE
                     GASEGKGLGLEFLRHVERCTALLHVLDCATLEPGRDPISDLDIILGELGAYPVPEGQL
                     PLLERAQLIALNKIDVPEARDLADMVRPELEARGYRVFEVSAASHEGLRELSFALAAL
                     VQADRAAKALEPVKQRIVIRPRAVDEKEFVVKVEGGTYGNVYNVVGTKPERWVAQTDF
                     ENEEAVGFLADRLAKLGVEDELFRAGAVPGSTVMIAGRVFDWEPTLSSTAELITSPRG
                     TDSRLDDNQRATNRARRQSYLERMDAKAAARAELEAERNAGIWTDDEGFAVSKEEDSE
                     "
     gene            2459..3262
                     /gene="proB"
                     /locus_tag="PLW15_09865"
     CDS             2459..3262
                     /gene="proB"
                     /locus_tag="PLW15_09865"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOT34737.1"
                     /translation="MSSVVTSRAGIPHARRIVVKVGSSSVSGENVGQIGALVDALAAA
                     HGRGSEVILVSSGAIATGIPYLNLDARPTDLATQQAAAAVGQNVLIFRYQESLDRYRI
                     VAGQILLTAGDMENPTHRSNAQRAMERLLGLRILPIVNENDTVATQEIRFGDNDRLAA
                     LVSVLVRADVLVLLSDIDALYTRPPAEPGALKIETVPFDDDLGGVTFGDIGAAGVGTG
                     GAGTKVAAAKLAAASGIPTLLAATGSVAEALAGAELGTWFEAAPPQPVE"
     gene            3306..>4002
                     /locus_tag="PLW15_09870"
     CDS             3306..>4002
                     /locus_tag="PLW15_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT34738.1"
                     /translation="MPETAVATSFTDKLTASREAARALATATTAQKNTALRAIADAVL
                     RNQARVLQANELDLANGRDNGLSAGLQDRLRLDEKRLIALSQAVLEIVALPDPVGDVV
                     RGSSLENGLQLTQIRVPFGVVGAIYEARPNVTIDIAALALKSGNAVVLRGGTAAENTN
                     RALVEVIQSALEEAGLPIESVQTIDEFGREGAKELMRARGFVDVLIPRGSADLINSVV
                     AESTVPVIETGAGV"
BASE COUNT          681 a         1388 c         1306 g          627 t
ORIGIN      
        1 gtcgttcctc ggtgtcgtca cagttctcgg ccagctggcc ttggtcgcca tgggcggcct
       61 gatcatcctc tccagcctgg tcgtgctggc ggggatcgca ttcccggcgc ttctggcgcc
      121 gttgttcccc tagcgggcgc cgtttgaccc agcaccccga agtcgcgtat tgttgactct
      181 tggtgtctgc tgggccaagc ccgcattcga tcaccaacag cttccaagag ttagggaaac
      241 caagtggttt acgcagttgt gcgcgccggt gggcgtcagg agaaggtcga ggttggctcg
      301 atcgtcgttc tcgaccgcat ccaggccgac aagaacggca acgtcgagct cgttcccgtg
      361 ctcttcgtcg atggtgacaa gatcacccac gacgccaagt cgctgacgaa ggtcaaggtc
      421 gtcgccgagg ttctcggtaa cgagcgtgga ccgaagatcg tcatccagaa gttcaagaac
      481 aagaccggat acaagaagcg ccagggccac cgccaggagc tgactcgtat caagatcacc
      541 agcatcgcgt aggggtagac actcatggca cacaaaaagg gcgcatcgtc cacccgtaac
      601 ggccgcgact ccaacgctca gcgcctcggc gtgaagcgct tcggcggcca gaccgtgctc
      661 gccggcgaga tcatcgtccg ccagcgcgga acccacttcc acccgggcgc caacgtcggc
      721 cgcggtggcg acgacacgct gttcgcgctc gcggccggtg ccgtcgagtt cggcgccaag
      781 ggcggccgca aggtcgtcaa catcgtcact gcagactagg gtctcgatac gctcgctgcg
      841 cgagctactc gaccaccggt aggggagcgg gcttcggctc gcttttcccg tttaaggcaa
      901 caccgcaacc gcaacaccgc aatcagaagg aggagtgcca tggcgtcatt cgtcgaccaa
      961 gtgaccctcc acctgctggc gggtcacggt ggcaacggct gcgtgtcggt caagcgcgag
     1021 aagttcaagc cgctcgctgg ccccgacggc ggcaacggcg gagacggcgg cgacatcgtg
     1081 ctcgtgagtg acccccaggt caccacgctg ctcggcttcc accgcgctcc gcaccgcaaa
     1141 tccgagcacg gccagcccgg ccagggcgac cacaaggccg gcacctcggg cgaggacctg
     1201 attctctccg tgcccgtcgg caccgtcatc aaagacgccg agggcaacga gctcatcgac
     1261 atggatgccc cgggcatccg ctacgtcgcc ggccccggcg gtcagggcgg cctcggcaac
     1321 gccgcgctcg ccaccaccaa gcgcaaggcg cccggtttcg ctctgctggg aacgcccggc
     1381 ttcgagggcg acatcttcct cgagctgaag accgtcgcgg atgtcgcgct cgtcggctac
     1441 ccgtcggcgg gcaagtcgag cttggtcgcc gccctctccg cagccaagcc gaagatcgcg
     1501 gactacccgt tcacgaccct gcaccccaac ctcggcgtcg ttgagtcggg cgacacccgc
     1561 tacacgatcg ccgatgttcc cggactcatc gagggtgcga gcgaaggcaa ggggctcggc
     1621 ctcgagttcc ttcgtcacgt tgagcggtgc acggcgctgc tgcacgtgct cgactgcgcc
     1681 accctcgagc ccggtcgcga cccgatcagc gatctcgaca tcatcttggg ggagctcggc
     1741 gcctacccgg ttcccgaagg ccagctaccg ctgctcgaac gcgcccaact catcgccctc
     1801 aacaagatcg acgtgcccga ggcgcgggat ctcgccgaca tggtgcgccc ggagctcgag
     1861 gcgcgcggct accgcgtgtt cgaggtttca gcggcatccc acgaggggct gcgcgagctg
     1921 tcgttcgctc tcgcagcact cgtgcaggcc gaccgcgccg cgaaggcgct cgagcccgtc
     1981 aagcagcgca tcgtcatccg gccgcgcgct gtggacgaga aggagttcgt cgtcaaggtc
     2041 gagggcggca cctacggcaa cgtgtacaac gtcgtcggca ccaagcccga gcgctgggtc
     2101 gcgcagaccg acttcgagaa tgaggaagcg gtgggcttcc tcgccgaccg tcttgccaag
     2161 ctgggcgttg aggacgaact gttccgcgcc ggggccgttc ccggatcgac cgtcatgatc
     2221 gcaggccggg tcttcgactg ggagcccacg ctctcgtcga ccgccgagct catcacgagc
     2281 ccgcgcggca ccgactcgcg cctcgacgac aaccagcgcg cgaccaaccg tgcacgtcgc
     2341 cagagctacc tcgagagaat ggatgcgaag gccgccgccc gcgccgaact cgaggccgag
     2401 cgcaatgccg gcatctggac tgatgacgag ggtttcgcgg tctccaagga agaggacagt
     2461 gagtagtgtc gtgacctcgc gcgccggtat cccgcacgcg cgacgcattg tggtgaaggt
     2521 cgggtcgtcc tccgtgtccg gggagaacgt cggtcagatc ggtgcactcg tcgatgcact
     2581 tgcggccgcc cacggtcggg gctcggaagt gattctcgtc tcgtcggggg cgatcgccac
     2641 gggcatcccc tatctgaatc tggatgcccg cccgaccgat ctggcaacgc agcaggccgc
     2701 ggccgccgtc ggccagaacg tgctcatctt ccgctaccag gagtcactcg accggtaccg
     2761 catcgtggcg ggacagatcc tgctcaccgc cggcgacatg gagaatccga cgcaccgctc
     2821 gaacgcgcag cgcgcgatgg agcgtctgct cgggctgcgc atcctgccga tcgtcaacga
     2881 gaacgacact gtggcaacgc aggagatccg tttcggcgac aacgaccgtc tggccgccct
     2941 tgtgtcggtg ctcgtgcgcg ccgacgtgct cgtgctgctc agcgacatcg acgcgctgta
     3001 cacccggccg ccggcggagc cgggcgccct caagatcgag actgtgccgt tcgacgatga
     3061 cctcggcggt gtcaccttcg gcgacatcgg tgcagccggc gtcggcaccg ggggagcggg
     3121 cacgaaggtc gccgcggcga agcttgccgc ggcatccgga atcccgaccc tcctcgccgc
     3181 gacgggcagt gtcgccgagg ccctggcggg cgccgagctg ggcacctggt tcgaggctgc
     3241 gccgccccag ccggttgagt aggccgaagg ccgtatcgaa accccgacgc cgctaaactc
     3301 gagccatgcc tgagaccgcc gtcgccacgt cgttcaccga caagctcacc gcctcgagag
     3361 aggccgcccg cgccctcgcc accgcgacga cagcgcagaa gaacaccgct ctgcgggcga
     3421 tcgccgacgc cgtgctgcgc aatcaagccc gcgtgctgca ggctaacgaa ctcgacctcg
     3481 cgaacggccg tgacaacggc ctcagcgcgg ggcttcagga ccgtctgagg cttgacgaga
     3541 agcgcctcat cgcgctctcg caggccgtac tggagatcgt ggccctgccc gacccggtcg
     3601 gcgacgtcgt gcgcgggtcc agcttggaga acggtctgca gctcacccag attcgcgtgc
     3661 cgttcggcgt cgtcggcgcg atctacgagg cgcggcccaa cgtcaccatc gacattgctg
     3721 ccctcgcgct caagagcggt aacgcggtcg tgctgcgcgg tggcacggcg gccgagaaca
     3781 ccaaccgggc tctggtcgag gtcattcagt ccgcgctgga agaggccgga ctgccgatcg
     3841 agtctgtgca gacgatcgat gagttcggtc gcgagggcgc caaggagctc atgcgggcgc
     3901 gcggattcgt cgacgtgctc atcccgcgcg gcagcgccga cctcatcaac tcggtggtcg
     3961 ccgagtcgac cgttccggtc atcgagaccg gcgccggtgt cg
//
LOCUS       DAOSOU010000202         3898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_23418_length_3898_cov_5.453031, whole genome shotgun sequence.
ACCESSION   DAOSOU010000202 DAOSOU010000000
VERSION     DAOSOU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23418_length_3898_cov_5.453031"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PLW15_09875"
     CDS             complement(<1..1413)
                     /locus_tag="PLW15_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT34739.1"
                     /translation="MLISLILRFLKPHWPLIVGVVVFQLAQSIASLYLPSLNADIIDK
                     GIATGDTNYILGVGAVMLGITLVQVAAAITAVYFGAKAAMKVGRDIRGAIFQRVGEFS
                     EREVSKFGAPSLITRTTNDVQQVQLLVLMTCTLLVSAPILAIGGIIMALQQDLELSWI
                     MVIAIPVLLLSIGLIISRMVPLFRLMQTRIDTVNRVLREQLAGIRVVRAFVREGLETE
                     RFAKANEDVTQTAISAGRLFALVFPVVLLIMNVSSVAVLWFGAFRIDDGSMQIGALTA
                     FLTYLIQILMAVMMATFMAILIPRAAVSADRIGQVLATETSVVLPEHPVTDLVGKGHI
                     ELRDVEFTYPGAEHPVLRNISFEARPGQTTAIIGSTGAGKTTLVNLLPRLFDATGGSV
                     MLDGVDVKLLDADLLWGRIGLVPQKAYLFSGTVASNLRYGKPDATDEELWRALEVAQA
                     KEFVEAMPEGLESPIAQGGTN"
     gene            complement(1415..2002)
                     /locus_tag="PLW15_09880"
     CDS             complement(1415..2002)
                     /locus_tag="PLW15_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT34740.1"
                     /translation="MSTTGSRARPLAPEDRREMIIDAVIPLILEHGVGITSRQIAEAA
                     GVAEGTVFRAFGDKESLIAAAADAFFERGASGESGPLIDASLGLEEKLEILVDVMRHR
                     VRDVMRMAMLTGRRGPTPSAEQIERFNRVISDAFAPDAHRLRVAPERLAQYVRVLAIA
                     TSIPVGGPELTDAEIVELITRGILVSPEAAHPKKD"
     gene            2098..3159
                     /locus_tag="PLW15_09885"
     CDS             2098..3159
                     /locus_tag="PLW15_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34741.1"
                     /translation="MIDQNGALLPNGLPQARARLGVGQGIAIAVGLVISVAASAGVGW
                     VAFNQQRVVDQAAVWQYAPDAAIASYAQRTDLTDEGSFLFFASRPQVSTGQLFNDICS
                     AHQEDVGILGCYVPTTRQIHLYDVTDPRLDGLEEVVAAHELLHAAWDRMSAAERNALA
                     PLLEAEAAEHADDADFAATLEFYQRTEPGERLNELHSIIGTEFTGLDDALEAHYARYF
                     ADRSAVTALHEQSNRVFLEQAAAVRGLTAQIDALAASIDSDYSAYNSAYDQLNSDIDA
                     FNARASAGDFSSQDQFDRARGELLERQDELDAQYAAIEANVNQYNSLVAQLDALNATT
                     EELNAAINIAPHPTAESAG"
     gene            complement(3156..>3898)
                     /locus_tag="PLW15_09890"
     CDS             complement(3156..>3898)
                     /locus_tag="PLW15_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT34742.1"
                     /translation="LAGRRDRFVLATKFGHAQGDMGIADGAPKGSRAYIRAAVADSLR
                     RLQTDHIDLYQLHTPDPVTPIEETIGALTELVTEGTIRAFGHSNFTAEQVVEAEQAAV
                     RLGGARFESAQNEFSLLVRDAERELLPSVRRYGLGFLPFFPLYNGLFTGKFTRTERPS
                     DTRIMRQRPHLADTAPWDTIERYEVFCAEHGVTMLSATIGWLLAQPGLTSVIAGATGP
                     EQLRQNAAASREWTPTADEVATISGIFA"
BASE COUNT          690 a         1299 c         1270 g          639 t
ORIGIN      
        1 gttggtacca ccctgcgcta tcggcgactc cagcccctcg ggcatcgcct cgacgaactc
       61 cttggcctgc gcgacctcga gcgcccgcca gagttcctcg tcggtggcat ccggcttgcc
      121 gtagcgcagg ttgctggcca cggttccgga gaacaggtac gccttctgtg gcaccagacc
      181 gatgcgcccc cagagcaggt cggcgtcgag cagcttcacg tcgacaccgt cgagcatcac
      241 gctgcccccg gtcgcgtcga acaggcgcgg gagcaggttg accagcgtgg tctttcccgc
      301 gccggtgctg ccgatgatgg cggtggtctg gcccgggcga gcctcgaagg agatgttgcg
      361 cagcaccgga tgctcggcgc cggggtaggt gaactccacg tcgcgcagct cgatgtgccc
      421 cttgccgacg aggtcggtga ccggatgctc cggcagcacc accgacgtct cggtggccag
      481 cacctgcccg atccgatcgg ccgacaccgc cgcgcgcggg atgaggatcg ccatgaaggt
      541 cgccatcatg accgccatca gaatctggat caggtaggtg aggaaggccg tgagggcgcc
      601 gatctgcatg gatccgtcat cgatgcggaa ggcaccgaac cagagcacgg cgacgctcga
      661 cacgttcatg atcagcagca cgacggggaa gaccagggcg aacaggcggc ccgcgctgat
      721 ggcggtctgg gtgacatcct cgttggcctt cgcaaaccgc tctgtctcga ggccctcgcg
      781 cacgaacgcg cgcaccaccc ggatgcccgc gagctgttcg cgcagtacgc ggttgaccgt
      841 gtcgatccgg gtttgcatga ggcggaacag cggaaccatg cgcgagatga tcaacccgat
      901 cgagagcaga agcacgggaa tcgcgatcac catgatccac gacagctcca gatcctgctg
      961 aagagccatg atgatgccgc cgatcgcgag gatcggtgcc gacaccagca gcgtgcaggt
     1021 catgagcacg agcagttgca cctgctgcac gtcgttggtg gtgcgggtga tgagcgaggg
     1081 cgccccgaac ttggagacct cccgctcgga gaactcgccc acgcgctgga agatcgcgcc
     1141 gcgtatgtcc cgcccgacct tcatcgctgc cttggcaccg aagtacacgg ccgtgatcgc
     1201 cgcggcgacc tgaacgagcg tgatgccgag catgaccgcc ccgacaccga ggatgtagtt
     1261 ggtgtcgcct gtggcgatgc ccttgtcgat gatgtcggcg ttcagactcg gcaggtagag
     1321 cgaggctatc gactgtgcga gttggaagac cacgacaccc acgatgagcg gccagtgcgg
     1381 cttgagaaac cgcaggatga gtgagatcag catgtcagtc cttcttcggg tgggcggcct
     1441 cgggtgagac caggatgccg cgggtgatga gttcgacgat ctccgcgtcg gtgagttcgg
     1501 ggccaccgac cggaatcgag gtcgcgatcg cgaggacccg cacatactga gcgaggcgct
     1561 cgggtgccac cctcaggcgg tgggcgtcgg gagcgaaggc atcggagatg acccggttga
     1621 agcgctcgat ctgctctgcg ctcggagtcg gtccgcgacg gccggtgagc atcgccattc
     1681 tcatgacgtc ccgcacgcgg tggcgcatga catccacgag tatctccaac ttctcttcga
     1741 gaccgagtga ggcgtcgatg agtggacccg attcgccgct ggcgcctcgc tcgaagaacg
     1801 cgtcggcagc ggccgcgatc aggctctcct tgtcaccgaa ggcgcgaaag accgtacctt
     1861 cggcgacacc cgccgcctcg gcgatctgcc gcgaggtgat gcccacaccg tgctcgagga
     1921 tgagcgggat cacggcgtcg atgatcatct cccgacggtc ctcgggggcg aggggccgcg
     1981 cgcgcgaacc tgtggtggac accgcaccat agtaagtgag tgagtactca ctccgccact
     2041 cccggaggca cccaccatga ggaatcttga cgccgggtcg gatagagtcc tgcggtgatg
     2101 atcgaccaga acggggcgct gcttcccaac gggctgcccc aggcccgcgc ccgactggga
     2161 gtcggccagg gcatcgccat cgcggtaggc ctcgtgatct cggtggcagc atccgccggg
     2221 gtcggctggg tcgcgttcaa ccagcagcgt gtggtcgatc aggctgcggt ctggcagtac
     2281 gcacccgacg ctgcgatcgc gtcctatgct cagcgcaccg acctcaccga cgagggctcg
     2341 ttcctgttct tcgcgagtcg accgcaggtc tcgaccggcc agctcttcaa cgacatctgc
     2401 tccgcgcacc aggaggatgt ggggatcctc ggatgctacg tgcccaccac ccggcagatt
     2461 cacctctacg acgtgaccga tccccgcctc gacggtctcg aagaggtcgt tgccgcccac
     2521 gagttgctcc acgcggcatg ggatcgcatg agcgccgcgg agcgtaatgc cctcgccccg
     2581 ctgctggagg ccgaggcagc cgagcacgcc gacgacgccg atttcgccgc cacactcgag
     2641 ttctaccagc gcaccgagcc cggtgagcga ctgaacgagc tgcactcgat catcggcacg
     2701 gaattcaccg ggctggatga cgcgctggag gcgcactacg cccgctactt cgccgatcgc
     2761 tcagcggtga ccgcccttca cgagcaatcg aatcgggtgt ttctcgaaca ggccgcggca
     2821 gtgcgggggc tcaccgcgca gatcgacgcg ctcgcggcat ccatcgactc tgactattcc
     2881 gcgtacaact cggcctacga ccaactcaac tccgacatcg atgccttcaa cgcgcgggcg
     2941 agcgccggag acttcagctc gcaggatcag ttcgatcgcg ctcggggcga actcctcgag
     3001 cgccaagatg agctcgacgc ccagtacgcc gcaattgagg cgaacgtgaa ccagtacaac
     3061 tcgctggtcg cccagcttga cgccctcaac gccacgaccg aggaactcaa cgcagccatc
     3121 aatatcgcgc cccaccccac cgccgaatcg gcgggctagg cgaagatccc cgagattgtc
     3181 gcgacctcgt ctgcggtcgg cgtccactcc cgggatgccg cggcgttctg gcgcagctgc
     3241 tccggaccgg tcgccccggc aatcacgctg gtgagccccg gctgagccag gagccagccg
     3301 atcgtcgccg agagcatcgt cacaccgtgt tctgcacaga acacctcgta gcgttcgatg
     3361 gtgtcccagg gagcggtgtc tgcgaggtgc ggtcgctggc gcatgatccg ggtgtcactg
     3421 gggcgctcgg ttcgggtgaa cttgcccgtg aacagtccgt tgtacagcgg gaagaacggc
     3481 agaaacccca gcccgtagcg tcgcacgctc ggcaggagtt cacgttcggc gtctcgaacg
     3541 agcaggctga actcgttctg agccgactcg aagcgcgctc cccccaaccg caccgccgcc
     3601 tgctcagcct cgacgacctg ctcggcggtg aagttggaat gcccgaatgc cctgatcgtg
     3661 ccctcggtga ccagctcggt cagagccccg atcgtctcct cgatcggcgt gaccgggtcg
     3721 ggtgtgtgca gctgatacag atcgatgtga tcggtctgca gtcgacgcag ggagtcggcg
     3781 acggccgccc gaatgtaggc gcgcgatccc ttgggtgctc catcggcgat ccccatatct
     3841 ccctgcgcgt gaccgaactt ggtggccagc acgaatcgat cccgccggcc ggccaagg
//
LOCUS       DAOSOU010000203         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_24176_length_3799_cov_4.371795, whole genome shotgun sequence.
ACCESSION   DAOSOU010000203 DAOSOU010000000
VERSION     DAOSOU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24176_length_3799_cov_4.371795"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1254
                     /locus_tag="PLW15_09895"
     CDS             271..1254
                     /locus_tag="PLW15_09895"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOT34743.1"
                     /translation="MFVAVIAAFHVIASHLTVGAAWFNFYVERRAYRENRPELYQYLR
                     KSAMGLLVFSYVWGAMTGVGIWQSTTAGNPRGISTLIHNFVLFWGSEWYMFLIDVVGI
                     IVYYYTLEKVDPKTHLRLAFILALGGTGTLSLIVGILGFKLSPGKWLENGEVLSGFYN
                     PTFWPQYFMRFALMQAITGAWAILMASRLPRDFPERRHIIRWAGIIGLVGLAAALLIG
                     RVWYWHNIPARSKGLLSTKAFPAVTVQILVVGLVFTALGLLLAAWRPSRHTRVSAIGM
                     FFVLFTTVFGAERTREILRKPDVVNGYISSNQLVFNGLPARGIESEEARLD"
     gene            1345..1668
                     /locus_tag="PLW15_09900"
     CDS             1345..1668
                     /locus_tag="PLW15_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34744.1"
                     /translation="MGRQIAIMECSGCHSVSDQTVIHVGDTQVALRQQSALLRKISAN
                     DAASIRALIDNLGTYPFMHDFVGNDDEKDALAAYLEYMVDRQQGPPTTLPPTTIAPTG
                     GSHGG"
     gene            1658..2863
                     /locus_tag="PLW15_09905"
     CDS             1658..2863
                     /locus_tag="PLW15_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34745.1"
                     /translation="MEVDDALRMMRDPLGAPFYPQVFQVLLVITWVIHIFFVTLAVGA
                     SVSTIWGFRRGGEYELRLARSTARLTPSAVGLGIVTGIAPLLFLQTIYDPIWYASNAL
                     TGFWSVIFILVVASGYTCAYVFYLKGSPEGRLLWTSVAGLVLLLLAGWIMHVLASVSI
                     RPEQWRQWYAPDGVADTRGIQFHAANAGRVTFLLPLQGALSVAIAGLVYTWYRSKRAD
                     ADREFLTWVAARARRVGIVASLLYSAAGVWWGLTEGAEMKVGIEVTVTLGALGLALAA
                     FFALHRNPVAIGPRAIAAWFGSLAVVAVVREVIRSSALAEFGYRVADYPFRVDWGSVA
                     MFGVTSVVGVSIIVYLVLVLYQAGLRSADQATISLRVDRFGRVATGMLGAWFGFFLLV
                     GLYAVLVLK"
     gene            2892..3593
                     /locus_tag="PLW15_09910"
     CDS             2892..3593
                     /locus_tag="PLW15_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34746.1"
                     /translation="MIPRQSTAFLAFSVVSWAAALVYGYSTGGDRLGPVLLGWWGGVG
                     DHLGYAVLIGAALLFLLAATVTILGRDGTDLTAEAQAQLGVAEVTPVEPASDALVYWP
                     FVTAVGMAVIVVGVVVDYALFVLGLVIVLAAGLAWAESNARERSRLLDVQPAEPPGAA
                     APRPLPAWAQGVLARVPAVAITLGFLSIVWGIFSNHEGGLGIVAGASVIAAGALASGF
                     VNVDPERPSSETSTA"
BASE COUNT          541 a         1208 c         1296 g          754 t
ORIGIN      
        1 ggcctgggcg tcggcggatt ctcggcgtgg cgactgatcg cgacccgagc gcaggacccg
       61 atcgccactc cgttgacctg agtttgccga ggcgagccgg ttcgacgccc caacgatgtc
      121 gactatctgc cgtggcgatg gaaccgttgg gttcatctcg tcgacctgga cttctggaac
      181 gtgcatgcgg ggctttgccc tcagccgcac tcgtggaagg accgtgaagc gatgtaccca
      241 acctgggagg gattgggcgt tcattcgacg atgttcgtcg cagtgatcgc cgcatttcat
      301 gtcattgcgt ctcacctcac ggtgggtgcg gcgtggttca acttctacgt cgaacgacgg
      361 gcctatcggg agaaccgtcc ggagctgtat cagtaccttc gcaagagcgc gatgggtctc
      421 ctggtcttct cctacgtgtg gggggcgatg accggcgtcg ggatctggca gagcaccaca
      481 gcaggaaacc cccgcggcat ctcgacgctc atccacaact tcgtcctctt ctgggggtcg
      541 gagtggtaca tgttcctgat cgatgtcgtc gggatcatcg tgtactacta caccctcgag
      601 aaggttgatc cgaagacgca cctgcgcctg gccttcatcc tcgcgctcgg cggaaccggc
      661 acgctgtcgc tgatcgtcgg catcctcggc ttcaagctct cgccggggaa gtggctcgag
      721 aacggcgagg tgttgtcggg gttctacaac cccacgttct ggccgcagta cttcatgcgg
      781 ttcgccctga tgcaggcgat cactggggcg tgggcgattc tcatggcatc ccggttgcct
      841 cgggacttcc cggagcgtcg gcacatcatc cggtgggccg gcatcatcgg cctcgtcggg
      901 ctcgctgcgg cgctcctgat cgggcgggtg tggtactggc acaacatccc cgcccgctcc
      961 aaggggctcc tctcgaccaa ggcgtttccg gcggtcacgg tgcagatcct cgtggtcggt
     1021 ctcgtcttca ccgcactggg actgctcctc gccgcatgga gaccctcgcg tcacacgcgt
     1081 gtcagcgcca tcggcatgtt cttcgtcctg ttcaccaccg tcttcggggc ggagcgcacg
     1141 cgggagatct tgcggaagcc ggacgtggtc aacggataca tctcgtccaa ccagctggtg
     1201 ttcaatggcc tcccggcacg ggggatcgaa agtgaagagg cccgcctcga ctagaccgga
     1261 attctcggac atctcccgtt cgtcccggcg gccgatgaga tcgacgccaa cgcgccgaag
     1321 gagttcccct cgcacgagat agcggtgggg agacagatcg ccatcatgga atgctcgggc
     1381 tgccacagcg tgagtgacca gacggtgatc cacgtcggag atacgcaggt ggcgctgagg
     1441 caacaatcgg cgttgttgcg caagatctcc gcaaacgatg ccgcgtcgat tcgtgcgctg
     1501 atcgacaatc tcggcaccta cccgttcatg catgacttcg tcggaaatga cgacgagaag
     1561 gacgcattgg cggcctacct cgagtacatg gtcgatcgac aacagggtcc gcccacgacg
     1621 ttgccgccga cgacgatcgc acccacggga ggctcacatg gaggttgatg acgcgctccg
     1681 gatgatgcgc gacccgctcg gcgcgccgtt ctacccgcag gtgttccagg tgctgctcgt
     1741 gatcacgtgg gtgatccaca tcttcttcgt gaccctcgcg gtcggcgcaa gcgtgtcgac
     1801 gatctgggga ttccggcgag gtggggagta cgagctccgg ctcgctcggt cgacggcgcg
     1861 gctgacaccg agcgccgtcg ggctcggcat cgtgaccggg attgcccccc tgttgttcct
     1921 gcagacgatc tacgacccga tctggtacgc cagcaacgcg ctcaccgggt tctggtcggt
     1981 gatcttcatc ctggtggtcg cgtcggggta cacctgcgcc tacgtcttct atctgaaggg
     2041 ttcgcccgag ggtcgtctgc tgtggacctc ggtcgccggg ctcgtcctgc tccttctcgc
     2101 gggctggatc atgcacgtgc tggcgagcgt ctccatccgg ccggagcagt ggcgacagtg
     2161 gtacgcaccc gacggggtcg cggatactcg ggggatccag ttccacgcgg cgaacgccgg
     2221 gcgggtgacg ttcctgcttc cgttgcaggg ggcgctgagc gttgcgatcg ccggtctggt
     2281 ctacacgtgg taccggtcca agcgcgccga cgccgatcgt gagttcctca cgtgggttgc
     2341 cgctcgagcc cggcgtgtcg gaatcgtcgc gagcctcctc tacagcgcag cgggagtgtg
     2401 gtggggactc accgaaggtg cggagatgaa ggtcggcatc gaggtgaccg tcacgctcgg
     2461 ggcgctcggc ctggcgctcg cggcattctt cgcgcttcac cggaatccgg tggccatcgg
     2521 gcctcgggcg attgctgctt ggttcggctc gctcgcggtc gtggcggtgg tgcgcgaggt
     2581 gatccgatcg tccgccctcg ctgagttcgg ctaccgcgtg gccgactacc cgttccgcgt
     2641 cgattggggg tcggtcgcga tgttcggggt gacctcggtg gtgggagtgt cgatcatcgt
     2701 gtacctcgtc ctggtgctct accaggcagg gcttcgctcg gccgaccagg ccacgatctc
     2761 cctgcgcgtc gaccgattcg ggcgggtcgc gaccggcatg ctcggagcct ggttcggctt
     2821 cttcctcctc gtcggcctct acgccgtact ggttctcaag tgatgcggat gacattcgaa
     2881 aaggaggcgc catgatcccg cgccagtcca ccgccttcct cgccttctcg gtcgtctcgt
     2941 gggcggccgc actggtctac ggctattcga cgggcggcga ccggctgggg ccggtgctgc
     3001 tcggctggtg gggaggagtc ggagaccatc tgggctacgc cgtgctgatc ggggctgcac
     3061 tgctgttcct gttggcggcc accgtcacga tcttgggccg tgacggcacc gacctgaccg
     3121 ccgaggcgca ggcgcagttg ggcgtcgccg aggtgacgcc ggtggaaccg gcatcggacg
     3181 cgctcgtgta ctggccattc gtcaccgccg tggggatggc tgtgatcgtg gtcggcgtcg
     3241 tcgttgacta cgcgttgttc gtgctggggc tggtcatcgt tctggcagcc ggcctcgcgt
     3301 gggccgagtc gaatgcgcgg gaacggagtc gcttgttgga cgttcaaccc gccgagccgc
     3361 ctggggccgc tgctccgcgg ccgctgccgg cctgggccca gggggttctg gcccgggtcc
     3421 cggcggtggc gatcacgctc gggttcctct cgatcgtctg ggggatcttc agcaaccacg
     3481 aaggtgggct cggcatcgtg gccggcgcca gtgtcatcgc agcgggcgcg ctggcatcgg
     3541 ggttcgtgaa cgtcgatccc gagcgcccgt catcggagac ctcgacggcc tgaacctccg
     3601 gtcccggttc gtcaccgacg aaccgggacc ggcggatcgc ttcgggtgtt gctcgtcagg
     3661 cgacgccgtt cgggcgacta caggctggcc ggggcgcggc ggtcggccca gttcgcgtcg
     3721 acgacctgtc tgccgaacgg ccagagcgcc agcaccccga tcttcactga ctgcacggcg
     3781 aacgggatgc cgatcaccg
//
LOCUS       DAOSOU010000204         3797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_24197_length_3797_cov_6.168894, whole genome shotgun sequence.
ACCESSION   DAOSOU010000204 DAOSOU010000000
VERSION     DAOSOU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24197_length_3797_cov_6.168894"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1189
                     /locus_tag="PLW15_09915"
     CDS             131..1189
                     /locus_tag="PLW15_09915"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT34747.1"
                     /translation="MSNGVRIGVVGATGQVGAVVRRLLEERDFPVEEIRFFASERSAG
                     TTLPFKGEEILVEDAATADPTGLDIAIFSAGATTSKAQAPRFAEAGVLVIDNSSGWRM
                     DPDVPLVVSEVNPHAIKDARKGIIANPNCTTMAAMPVLKVLDAEAGLQRLIVSTYQAV
                     SGAGLAGGEELLEQAKAAVAQDAIELVHDGSAVIMPTPSKFPKNIAFNVVPFAGNLVD
                     DGLGETDEEKKLRNESRKILELPDLLVSGTCVRVPVFTGHSLSINAEFARPVSPERAR
                     ELLDGAAGVVLSDIPTPLEAAGNDPSYVGRIRQDEGVPEGRGLALFISNDNLRKGAAL
                     NAVQIAELVAADLAAATA"
     gene            1226..1873
                     /locus_tag="PLW15_09920"
     CDS             1226..1873
                     /locus_tag="PLW15_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT34748.1"
                     /translation="MRSLFLLGLGLVLALAGCTAVTPPADDDNATRVAFYGDSYTLGT
                     GASDPSLRWSSIICAERGWQEFNPSVNGLGFVNNRATDGEGDLPALVIANDPGIVFIT
                     MGLNDNFSYDRAANQIRLRITEDFTRLATALPDARFIVVEPFWYTDRRPESVEVIIGW
                     VRDAAAAIGADYIPGASHWIEGHPEWMASDGLHPNDAGYAAMAARMDAELTKLGL"
     gene            1894..3369
                     /locus_tag="PLW15_09925"
     CDS             1894..3369
                     /locus_tag="PLW15_09925"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HOT34749.1"
                     /translation="MSKPVDVALIGGGIMSATLGMLLKQLEPSWDIQIYERLGDVALE
                     SSNPWNNAGTGHSALCELNYTPERADGTIDISSAVKVNEQFQVSRQFWSHLVGSGQLP
                     EPSQFINSTPHMSFVWGEANVEYLRKRYEALKDHPLFAGMEFSTDARTIRSWTPLMIP
                     GRKKSQPIAATRIASGTDVDFGALTHHLTSSLIAGGAGFTTEVKVTNLSRQRDGLWKL
                     SLRSDLGGTPLAPVTARFVFVGAGGYALGLLQKSGIKEIRGYGGFPVSGEFLRTDNPD
                     LVAQHRTKVYGKAAVGSPPMSVPHLDLRVVDGTGSLMFGPYAGFSPKFLKTGSVLDLF
                     ASIRWHNIVPMVAAGLSNLGLVKYLIGQLAARQSTKFAELQQFVPNAKPEDWYRITAG
                     QRVQVIKKDKKKLGVLQFGTEVVAAADGSIAGLLGASPGASTAVPIMVSLVEKCFPDR
                     IEAWRPALVGMIPSYGKHLSDDPKLAEKTLASTAKVLGIHP"
     gene            3408..>3797
                     /locus_tag="PLW15_09930"
     CDS             3408..>3797
                     /locus_tag="PLW15_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT34750.1"
                     /translation="MRWNGQQLDPERTDALPGLARLSNLVRSVTTPEFAGVRFHEVLA
                     KSALNRVPGESAMPFGWTINPYRGCSHACVYCLSGETQVLMADGRQKPLWAVRVGDRV
                     VGTEQRGRYRRYIATDVLAHWSTRKPAF"
BASE COUNT          657 a         1285 c         1233 g          622 t
ORIGIN      
        1 gctgacccct tcgtcggtga tcgagcgaag tcgagatccc ggcaggctcg atcaccgctg
       61 gcagcatctc gacggctgct gacggctcac tccgcagagc ctgcgccgcg agcatgccac
      121 aattgttccc atgagtaacg gtgtgagaat cggcgtcgtg ggcgccaccg gtcaagtggg
      181 cgccgtcgtg cgtcgtctcc tggaggagcg ggacttcccg gtcgaggaga tccgattctt
      241 cgcgtcagag cgcagcgccg gcacgaccct gcccttcaag ggtgaggaga tcctcgtcga
      301 ggatgcggcg accgcagacc cgaccggcct cgacatcgcg atcttctccg cgggagccac
      361 caccagcaag gcgcaggcac cgcggttcgc cgaggcgggc gtgctcgtca tcgacaactc
      421 gtccggctgg cgcatggacc cggatgttcc tctggtcgtc agcgaggtca acccccacgc
      481 gatcaaggat gcccgcaagg gcatcatcgc caaccccaac tgcacgacga tggccgcgat
      541 gcccgtgctc aaggtcctcg acgccgaggc gggactccag cgtctgatcg tcagcaccta
      601 ccaggcggtc tccggcgcag gtctcgcggg cggcgaggag ctgctcgagc aggccaaggc
      661 cgccgtcgcg caggacgcga tcgagctcgt gcacgacggc tcggccgtca tcatgccgac
      721 cccgtcgaag ttcccgaaga acatcgcctt caacgtggtg ccgttcgcgg gcaacctcgt
      781 cgacgacggt ctgggagaga ccgacgagga gaagaagctg cgcaacgaga gccgcaagat
      841 cctcgagctg cccgacctgc tggtcagcgg cacctgtgtg cgtgttcccg tgttcaccgg
      901 gcactccctg agcatcaacg ccgagtttgc gaggccggtg agcccggagc gtgcgcgcga
      961 gctgctggat ggcgcggccg gggttgtgct cagcgacatc ccgaccccgc tggaggccgc
     1021 cggcaatgac ccgagctacg tgggtcgcat ccgtcaggat gagggcgtgc ctgagggccg
     1081 cgggcttgcg ctgttcatca gcaacgacaa cctgcgcaag ggcgcagccc tcaacgccgt
     1141 gcagatcgcg gagctggtgg ctgccgacct cgccgcggcg acggcctaga ccgagccgaa
     1201 cggtgacggg cgccgaacac cgggtatgcg ctctctcttt ctgctcggcc tcgggctggt
     1261 actcgcactc gccgggtgca ctgctgtgac accgccggct gacgacgaca acgcgacgcg
     1321 cgtggccttc tacggcgact cgtacaccct cgggaccggc gcgagcgacc cgtcactgcg
     1381 ctggtcgtcg atcatctgcg cggagcgcgg ctggcaggag ttcaacccga gcgtcaatgg
     1441 cctcggattc gtcaacaacc gtgcgactga cggtgagggc gatctcccgg cgctcgtcat
     1501 cgccaacgac cccggcatcg tattcatcac catgggtctg aacgacaact tcagctacga
     1561 ccgagccgcc aaccagatcc gcttgcgcat caccgaggac ttcacccggc tggcgacggc
     1621 gcttccggat gcccgtttca tcgtcgtcga accgttctgg tacaccgacc ggcgccccga
     1681 atcggtggag gtcatcatcg ggtgggtgcg ggatgccgcc gccgccatcg gcgccgacta
     1741 catccccgga gcatcccact ggatcgaagg gcaccccgag tggatggcct ccgacggtct
     1801 gcaccccaac gatgccgggt acgccgccat ggcggcccgc atggatgccg agctgacgaa
     1861 actgggtctg taaaccagca ctaaactgta gtggtgagta aacccgtgga tgtcgccctc
     1921 atcggcggag gcatcatgag tgcaaccctc ggaatgctgc tcaagcagct ggagccgagc
     1981 tgggacattc agatctacga gcgtctcggc gatgtcgccc tcgagagctc gaacccgtgg
     2041 aacaacgcag gcaccggcca ctcggccctg tgtgaactca actacacgcc cgagcgggcc
     2101 gacggcacga tcgacatctc gagcgcggtg aaggtcaacg agcagttcca ggtgtcccgc
     2161 cagttctggt cgcacctcgt cggttccggc cagctgcccg agccgtctca gttcatcaac
     2221 tcgacgccgc acatgagctt cgtgtggggc gaggccaacg tcgagtacct gcgcaagcgg
     2281 tacgaggcgc tcaaggacca cccgctgttc gccggcatgg aattctcgac ggatgcccgc
     2341 accatccgtt cctggacccc gctcatgatc cccgggcgca agaagtcgca gccgatcgct
     2401 gcgacgcgca tcgcctcggg caccgacgtg gatttcggcg cgctcaccca tcacctcacc
     2461 tcgagtctca tcgcgggcgg cgcgggtttc accacagagg tcaaggtgac caacctcagc
     2521 cggcagcgcg acggcctgtg gaagttgagc ctgcgtagcg atctcggcgg aacgccgctt
     2581 gccccggtca ccgcgcggtt cgtgttcgtc ggtgccggtg ggtacgcgct cggcctgctg
     2641 cagaagtcgg gtatcaagga gattcgcgga tatggcgggt tcccggtgag cggcgaattc
     2701 ctgcgcaccg acaaccccga cctcgtcgcc cagcaccgca ccaaggtcta cggcaaggcc
     2761 gccgtgggtt cacccccgat gtcggtgccg cacctcgacc tgcgcgtcgt cgatggcacg
     2821 ggcagcctga tgttcgggcc gtatgcgggc ttcagcccca agttcctcaa gaccggctcg
     2881 gtcctcgatc tgttcgcctc gatccgctgg cacaacatcg tgcccatggt tgcggccggc
     2941 ctgtcgaatc tgggcctcgt caagtacctc atcggacagc tggccgcccg ccagtccacg
     3001 aagttcgccg aactgcagca gttcgtgccc aacgccaagc ccgaggactg gtaccgcatc
     3061 actgccggcc agcgcgtgca ggtgatcaag aaggacaaga agaagctcgg tgtgctgcag
     3121 ttcggcaccg aggtcgtggc cgccgccgac ggcagcatcg cgggcctgct cggagcatca
     3181 cccggcgcat ccaccgcggt gccgatcatg gtgagcctgg tggagaagtg cttccccgac
     3241 aggatcgaag cctggcgccc ggcgctggtg ggcatgatcc ccagctacgg caagcacctc
     3301 tccgacgacc cgaagctggc cgagaagacg ctcgcgagca cggcgaaggt gctcggcatc
     3361 cacccctagc ggttgacaga cattcgaaca catgttcgac tgtctggatg aggtggaacg
     3421 gtcagcagct cgaccccgag cgcactgatg cgctcccggg tctggcgcgt ctgtcgaacc
     3481 tggtgcggtc cgtgacgact cccgagttcg ccggcgtgcg ctttcacgag gtgctcgcca
     3541 agagcgcact caaccgcgtg cccggcgaga gcgcgatgcc gttcggctgg accatcaacc
     3601 cgtaccgcgg gtgcagccat gcttgtgtct attgtctgag cggtgagacg caagtgctta
     3661 tggcggacgg gcgccagaaa cccctgtggg cggtgcgggt tggcgaccgc gttgtgggca
     3721 ccgagcaacg tggtcgctac cgtcgataca ttgcgacgga cgttctcgct cactggagca
     3781 cgaggaagcc ggcgttt
//
LOCUS       DAOSOU010000205         3743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_24633_length_3743_cov_6.954176, whole genome shotgun sequence.
ACCESSION   DAOSOU010000205 DAOSOU010000000
VERSION     DAOSOU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24633_length_3743_cov_6.954176"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PLW15_09935"
     CDS             complement(<1..1389)
                     /locus_tag="PLW15_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT34751.1"
                     /translation="MAIATRSDLRNVAIVAHVDHGKTTLVDAMLRQTNSFAEHAHLEE
                     RAMDSNDLEREKGITILAKNTAISYKGKHATDGPVTINVIDTPGHADFGGEVERGLSM
                     VDGVVLLVDASEGPLPQTRFVLRKALEAKLPVVLLVNKTDRPDARIDEVVTESQDLLL
                     GLASDMHDDFPDLDLDAVLDVPVVYASGRNGAASWNKPENGTLPDNDDLEPLFDAIIK
                     HIPAPTYDDEHPLQAHVTNLDASPFLGRLALLRVFNGTIKKGQTVAWVKSDGSVHNVR
                     VTELLITKALDRFPTDSAGPGDIVAVAGFEDITIGETLADPEDVRPLPTITVDDPAIS
                     MTIGTNTSPLIGKIKGHKLTARMVKDRLDRELVGNISIKLLDIGRPDAWEVQGRGELA
                     LAILVEQMRREGYELTVGKPQVVTKYIDGKLHEPFEHLTVDSPEEHLGAITQLMASRK
                     GRMETMTNHGTGW"
     gene            complement(1402..3096)
                     /locus_tag="PLW15_09940"
     CDS             complement(1402..3096)
                     /locus_tag="PLW15_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT34752.1"
                     /translation="MTPTTTAAETVLEVTDLGVDFWVDGTFYPAAIDLNYVVKRGEVL
                     AIVGESGSGKSSSSMALLGLLPANAKVSGSVKLLGRELRGIPQAELRSVRGNQIAVIF
                     QEPMTALNPVYTIGFQIMEALRSHFDLNPTQAKERAIELLTKVEMPDPATAFYKYPHQ
                     LSGGQRQRAMIAQSISCDPVLLIADEPTTALDVTVQAEIIDLLRSLRERLDSAIILIT
                     HDMGVVADLADNILVMKDGLAVESGTVIDVFTKPQHPYTKSLLAAVPHLGTASAQVNE
                     QEPANASQKRTPVLQFADVAIDYPKRGRVPAFRAAEHINLEIYPGEILGLVGESGSGK
                     TTLGRASVGLLPIAEGTLSVVGYDISHANNKDLRPLRRKTGIVFQDPGSSLNPRMPIG
                     QSIGEPLLLAGEAKGKDLDKRVQELLSQVELPTSYRNRYPHELSGGQRQRVGIARALA
                     LTPELLVADEPTSALDVSVQARFLDLLQELQERLQFACLFVSHDLAVVDILAHRIAVM
                     HRGRLVEQGTRDEILRNPQDPYTQRLIAAVPLPDPIAQRERRETRVASKAQKGRTS"
     gene            3135..3737
                     /locus_tag="PLW15_09945"
     CDS             3135..3737
                     /locus_tag="PLW15_09945"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT34753.1"
                     /translation="MRFAPTSFRPAFGRVLSVVVVVMAIAGLASFVVTGDWLGLVRYG
                     WALALLGAGALAVFWFPRLDIAEHEVTVRNVFSTVHVPWPAIERVDTKWALTLHTAEG
                     TVTAWASPAPTRYASASASRRDASLSGDQQGFAPRPGDLPTTESGAAAYVIRQHWEDL
                     RADGLLASGVEPGSVRRDIHWATIAVLGGLLVASVLGVLL"
BASE COUNT          548 a         1177 c         1326 g          692 t
ORIGIN      
        1 ccaaccggtg ccgtggttcg tcatcgtctc catgcgaccc ttgcgcgacg ccatcagctg
       61 cgtgatcgcg ccgaggtgct cctccggcga atccaccgtg aggtgctcga acggttcgtg
      121 gagcttgcca tcgatgtact tggtgaccac ctggggcttg cccacggtca gttcgtagcc
      181 ctcgcggcgc atctgctcga cgaggatggc gagcgccaac tcaccgcggc cctgcacctc
      241 ccaggcatcc gggcggccga tgtcgagcag cttgatggag atgtttccca ccagttcgcg
      301 atcgaggcga tccttgacca tgcgggcggt cagcttgtgg cccttgatct tgccgatgag
      361 cgggctcgtg ttggtgccga tggtcatcga gatcgccggg tcgtcgaccg tgatggtcgg
      421 aagcggccgc acgtcctcgg ggtcggcgag ggtctcgccg atggtgatgt cctcgaagcc
      481 ggcgacggcg acgatgtcac cggggcctgc ggagtcggtc gggaaacggt cgagtgcctt
      541 ggtgatcaga agctcggtca cgcgcacgtt gtgcacgctg ccgtcgctct tgacccaggc
      601 cacggtctgg cccttcttga tcgtgccgtt gaacacgcgc agcagcgcga gacggccgag
      661 gaaggggctc gcgtcaaggt tggtgacgtg ggcctgcagg gggtgctcgt cgtcgtacgt
      721 cggagcgggg atgtgcttga tgatcgcgtc gaacagcggc tcgaggtcgt cgttgtcggg
      781 cagagtgccg ttctcgggct tgttccagct cgcggcgccg ttgcggcccg aggcgtagac
      841 caccggaaca tcgagcacgg cgtcgaggtc gaggtcgggg aagtcgtcgt gcatgtcact
      901 ggcgagtccg agcagcagat cctggctctc ggtcacgacc tcgtcgatgc gggcatccgg
      961 tcggtcggtc ttgttgacca gcagcacgac gggaagcttc gcctcgaggg ccttgcgcag
     1021 cacgaagcgg gtctggggca gggggccctc actcgcatcc acgagcagca cgacgccgtc
     1081 gaccatcgac aggccgcgct cgacctcgcc accgaagtcg gcgtggccgg gggtgtcgat
     1141 cacgttgatc gtcacgggac cgtcggtcgc gtgcttgccc ttgtacgaga tcgccgtgtt
     1201 cttggcgagg atcgtgatgc ccttctcgcg ctcgaggtcg ttcgagtcca tcgcccgctc
     1261 ctcgaggtgg gcatgctcgg cgaacgagtt ggtctggcgc agcatggcgt cgaccagggt
     1321 cgtctttccg tggtcgacgt gagcgacaat cgccacgttg cgcaggtcgg agcgtgtggc
     1381 gattgccatg gcagagttcc tttagctggt gcggcccttc tgggccttgg atgcgacgcg
     1441 ggtctcccgc cgctcgcgct gcgcaatcgg atcgggcagc ggcacggcag cgatgagtcg
     1501 ctgggtgtag gggtcttggg ggttgcgcag gatctcgtct ctggtccctt gctccaccag
     1561 tctaccgcga tgcatgaccg caatcctgtg cgcgaggata tcgacgaccg cgaggtcgtg
     1621 gctgacgaac aggcaggcga actgcaagcg ctcctgcagc tcctgcagca ggtcgaggaa
     1681 tcgggcctgc acggacacgt ccagcgcact cgtgggctcg tcagcgacga gcagttcggg
     1741 ggtcaatgcg agtgcgcgag cgatgccgac acgctgacgc tggccaccgc tgagttcgtg
     1801 cgggtagcgg ttgcggtagc tggtgggaag ctccacctgg ctcaggagct cctgcacccg
     1861 cttgtcgagg tccttgccct tcgcctcacc ggccagcagc agcggttcgc cgatggactg
     1921 gccgatcggc atgcgcgggt tgaggctcga gcccgggtcc tggaagacga tgccggtctt
     1981 gcggcgcagc ggtcgcaggt ccttgttgtt ggcgtgggag atgtcgtagc ccacgacgct
     2041 gagggtgccc tcggcgatcg gcagcaggcc gacggatgcc cgacccaacg tggtcttgcc
     2101 cgacccggac tcccccacca ggccgagaat ctcgccgggg tagatctcca ggttgatgtg
     2161 ctcggcggcc cggaaggcgg gcacgcggcc gcgcttgggg tagtcgatcg cgacatccgc
     2221 gaactgcagc accggagtgc gcttctgact cgcgttcgcg ggctcctgct cgttgacctg
     2281 cgcgctcgcg gtgccgaggt gcggcacggc ggccagaaga gacttcgtgt acggatgctg
     2341 cggcttcgtg aacacgtcga tgacggttcc cgactcgacg gccaggccat ccttcatcac
     2401 gaggatgttg tcggccaggt cggccacgac acccatgtcg tgagtgatca ggatgatggc
     2461 gctgtcgagg cgctcccgaa ggctgcgcag gaggtcgatg atctccgcct gcaccgtcac
     2521 gtcgagtgcg gtcgtcggct cgtcagcgat gagcagcacg gggtcgcagg agatcgactg
     2581 tgcgatcatc gcacgctggc gctggccgcc cgacagctgg tgggggtact tgtagaacgc
     2641 ggtcgcggga tccggcatct cgaccttggt gagcagctcg atcgcccgct ccttggcctg
     2701 ggtggggttc aggtcgaagt gcgagcgcag ggcctccatg atctggaagc cgatcgtgta
     2761 caccgggttc agggccgtca tgggctcctg gaagatcacg gcgatctggt tgccgcgcac
     2821 cgagcgcagc tccgcctgcg ggatgccacg aagctcgcgc ccgagcagct tgaccgaacc
     2881 gctgaccttg gcattcgcgg gaagcagccc gagcagtgcc atcgagctcg aactcttgcc
     2941 cgagccggac tcaccgacga tcgccagcac ctcgccgcgc ttgacgacgt agttcagatc
     3001 gatcgcggcc gggtagaagg tgccatcgac ccagaagtcg acgccgaggt cggtgacctc
     3061 gagaacggtc tctgctgcgg ttgttgtggg ggtcactggg gtacctctcc ggtgcggtct
     3121 gcgaagctag gggaatgcgc ttcgccccga cgagtttccg tccggccttc ggccgggtac
     3181 tgagtgtggt cgttgtagtt atggccatcg ctggcctcgc cagctttgtg gtcaccggcg
     3241 actggctggg actcgtgcgc tacggctggg ccctggcgct gctcggcgcg ggcgctctcg
     3301 cggtgttctg gtttccccgt ctggacatcg ccgaacacga ggtgacggtg cgcaacgtct
     3361 tcagcaccgt gcacgtaccg tggcccgcga tcgagcgagt cgacaccaag tgggctctca
     3421 ccctgcacac tgccgagggc accgtgaccg cctgggcgag cccagctccg acccgatacg
     3481 ccagcgcttc ggcgagccga cgtgacgcca gcctgtcggg agaccagcag ggcttcgcgc
     3541 cgcggcccgg agacctgccg accaccgaga gcggagctgc ggcgtacgtg atccgtcagc
     3601 actgggagga cctgcgcgcc gacggcctgc tggcctcggg tgtcgaaccg gggagcgtgc
     3661 gccgcgacat ccactgggcc accattgcag tgctcggtgg actgctcgtg gcgagcgtcc
     3721 tgggcgtact gctctagccg cat
//
LOCUS       DAOSOU010000206         3742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_24644_length_3742_cov_6.244372, whole genome shotgun sequence.
ACCESSION   DAOSOU010000206 DAOSOU010000000
VERSION     DAOSOU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24644_length_3742_cov_6.244372"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /gene="atpD"
                     /locus_tag="PLW15_09950"
     CDS             <1..880
                     /gene="atpD"
                     /locus_tag="PLW15_09950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOT34754.1"
                     /translation="SVFAGVGERTREGNDLIAEMEEAGVFDKTALVFGQMDEPPGTRL
                     RVALSALTMAEYFRDVQKQDVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPNLADE
                     MGILQERITSTRGHSITSLQAIYVPADDYTDPAPATTFAHLDATTELSREIASKGLYP
                     AVDPLTSTSRILDPRYLGADHYRVATNVKAILQKNKELQEIIAILGVDELSEEDKITV
                     SRARRIQQFLSQNTYMAKKFTGVEGSTVPLKDTIESFDAITKGEFDHVAEQAFFNVGA
                     ISDVEERWAQIQKENG"
     gene            883..1152
                     /locus_tag="PLW15_09955"
     CDS             883..1152
                     /locus_tag="PLW15_09955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOT34755.1"
                     /translation="MAELKVSIVSADQEVWSGKAKQIIARTLVGEIGILAGHEPILAI
                     LGTGEVRVTTIDGELITATADDGFLSVDNNNVTVVARNAELVSQG"
     gene            1170..1676
                     /locus_tag="PLW15_09960"
     CDS             1170..1676
                     /locus_tag="PLW15_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT34756.1"
                     /translation="MAGLPGTGKSTIGQVVASRLNIPVVSVDPIETAILQAGIAADQP
                     TGLAAYLVAETLAERVLSTGIGVMVDAVNAVAPAREQWVALAERMQEPIRFIEVICSD
                     PELHRSRIEGWRKRMPHIQLTWNAVEQSFDDYADWTGSSAAVARITLDSVDPLGVNVE
                     RAIAFLKS"
     gene            1676..>2141
                     /gene="yaaA"
                     /locus_tag="PLW15_09965"
     CDS             1676..>2141
                     /gene="yaaA"
                     /locus_tag="PLW15_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOT34757.1"
                     /translation="MLLLLPPSETKRDGGADARRLDLAELGFPALTPQRRAALAALAR
                     LSRSVGDSMTALGLGPTQRAEIDRNRQVRSSPVMPAIDRYTGVLYDGLDAASLTTAER
                     DFAGRHVAIHSALFGLLRSDDPIPAYRLSHDSRLPGLSLRSHWRSAVTTELAA"
     assembly_gap    2142..2241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2320..2901)
                     /locus_tag="PLW15_09970"
     CDS             complement(2320..2901)
                     /locus_tag="PLW15_09970"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOT34758.1"
                     /translation="MTDRASAVVGEDGLTRCAWVGNDPEYRRYHDEEWGRPLHGDQRL
                     FEKIALEGFQAGLSWITILRRRAGFREAFLGFDVARVAAMGPHDVEALMLDARIIRNR
                     AKIEATLSNARVTLEMNEGELDELVWSFAPPAGRRRPNSLGDIPAVTTESTALSKTLR
                     SRGFRFVGPTTMYALMQSAGLVDDHVVGCHRAD"
     gene            complement(2898..3419)
                     /locus_tag="PLW15_09975"
     CDS             complement(2898..3419)
                     /locus_tag="PLW15_09975"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT34759.1"
                     /translation="MQNHTTIDSPPPPSIHIARVDSPIGRIELTSDGTSITSLAIERD
                     GHLPHDGQPEASTAVIDTAIAQLSEYFAGTRREFDVPVTLGGTDFQRSVWRELTDIPF
                     GEVASYGEIGMATGRPTAGRAVGGAVGANPVPIIIPCHRVLASNGRITGYSGGNGIPT
                     KEWLLDHEGIEHR"
     gene            3514..>3742
                     /locus_tag="PLW15_09980"
     CDS             3514..>3742
                     /locus_tag="PLW15_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT34760.1"
                     /translation="MITLPHRELGRSGLQVSVVGLGCNNFGRPGSRTASQEGTTAVID
                     AADDLGVTLLDTADIYGGHPGQSETYLGVALA"
BASE COUNT          593 a         1229 c         1193 g          627 t
ORIGIN      
        1 gtcggtgttc gccggtgtcg gcgagcgcac ccgtgagggc aacgacctca tcgccgagat
       61 ggaggaggcg ggcgtcttcg acaagaccgc ccttgtcttc ggccagatgg atgagccgcc
      121 gggcacgcgt ctgcgcgtcg ccctctccgc gctgacgatg gcggagtact tccgcgacgt
      181 gcagaagcag gacgtgctgt tgttcatcga caacatcttc cgcttcacgc aggctggttc
      241 cgaggtctcg acgcttctgg gtcgcatgcc gtccgccgtg ggttaccagc ccaacctcgc
      301 ggatgagatg ggtatcctcc aggagcgcat cacctcgact cgcggtcact cgatcacgtc
      361 gctgcaggcc atctacgttc ctgccgacga ctacacggac ccggctccgg ccacgacgtt
      421 cgcgcacctc gacgccacga ccgagctcag ccgtgagatc gcgtcgaagg gcctctaccc
      481 ggccgtcgac ccgctcacgt cgaccagccg catcctggac ccccgctacc tgggtgccga
      541 ccactaccgc gtggcaacga acgtgaaggc gatcctccag aagaacaagg aactccagga
      601 gatcatcgcg atcctcggtg ttgacgagct ctccgaagag gacaagatca ccgtgtcgcg
      661 cgcgcgtcgc atccagcagt tcctctcgca gaacacctac atggccaaga agttcacggg
      721 cgtcgagggt tcgacagttc cgctgaagga caccatcgag tcgttcgacg cgatcaccaa
      781 gggtgagttt gaccacgttg ccgagcaggc cttcttcaac gtcggtgcga tctccgacgt
      841 cgaagagcgc tgggcgcaga tccagaagga gaacggctaa ccatggccga gctcaaggtc
      901 agcatcgtct cggccgatca ggaggtctgg tcgggcaagg cgaagcagat cattgcccgc
      961 actctcgtgg gtgagatcgg tattctggcg ggccacgagc cgattctggc tattctcggc
     1021 accggcgagg tgcgcgtgac gacgatcgac ggtgagctca tcacggcaac agccgacgat
     1081 gggttcctgt cggtcgacaa caacaacgtc accgtcgtcg cccgtaacgc cgagctggtc
     1141 agtcaggggt agcaaccgtg ctcgtcgcga tggcaggcct ccccggcacg ggcaagtcga
     1201 cgatcgggca ggttgtcgcg agtcggctga acatccccgt ggtgtcggtc gaccccattg
     1261 agaccgccat cctgcaggcc ggtatcgctg ccgaccagcc gacgggtctc gcggcgtacc
     1321 tcgtcgctga gacccttgcc gagcgggtcc tcagcaccgg aatcggtgtg atggtcgatg
     1381 ccgtcaacgc tgtcgcgccc gcgcgggagc agtgggtcgc cctggccgag cgcatgcagg
     1441 aaccgatcag gttcatcgaa gtgatctgct cagatcccga gctgcaccgc agtcgcatcg
     1501 agggctggcg caagcgcatg cctcacatcc agctgacgtg gaacgcggtc gagcagagct
     1561 tcgatgacta cgcggactgg accggatcga gcgcagccgt cgcccgaatc accctcgact
     1621 ccgtcgaccc gctcggcgtg aacgtcgagc gcgcgatcgc cttcctcaag agctagtgct
     1681 tctgctgctt cccccctccg agacgaagcg cgacggggga gcggatgccc gtcggctcga
     1741 tctcgccgaa ctggggttcc ccgccctcac tccgcagcgc cgagccgctc tcgcagctct
     1801 cgcgcggctc tcccggagcg tgggggactc gatgaccgct ctcggcctcg gacccacgca
     1861 gcgcgcggag atcgaccgca atcgtcaggt gcgcagctcg cccgtgatgc cggcgataga
     1921 tcgctacacc ggtgtgctct acgacggcct ggatgcggca tccctcacga ccgccgagcg
     1981 ggacttcgcg gggcgtcatg tcgccatcca ctccgccctg ttcggactcc ttcgctcgga
     2041 tgacccgatc ccggcctacc gcctgtcgca tgactcacgc ctcccgggtc tgtccctgcg
     2101 cagccattgg cggtccgcgg tgaccacgga gctcgccgcc cnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn natccggctg gagaagcagg gcaccggcga gctcgatctc
     2281 gtgcagtagc cacgttcgct tcggcgctca gccctcgagt cagtcggccc ggtggcatcc
     2341 cacaacgtgg tcgtcgacga gcccagcgga ctgcatgagc gcgtacatcg tggtgggtcc
     2401 gacgaaccgg aacccgcgag agcgcaacgt cttgctcagc gccgtggact cggtcgtgac
     2461 cgccggtatg tccccgagtg agttgggcct gcgcctgccc gcgggcggcg cgaaactcca
     2521 gaccagctcg tcgagttcac cctcgttcat ctcgagggtc acccgcgcgt tggagagcgt
     2581 ggcctcgatc ttcgcgcggt tgcggatgat gcgcgcgtcg agcatcagcg cctccacgtc
     2641 gtgcggcccc atggcggcaa ctcgcgcgac gtcgaacccg aggaacgcct ccctgaagcc
     2701 cgcgcggcgc ctcaatatgg tgatccagga taggccggcc tgaaagccct cgagcgcgat
     2761 cttctcgaac aggcgctggt caccgtgcag gggtcgcccc cactcctcgt cgtggtaccg
     2821 gcggtactcg ggatcgttgc ccacccatgc gcagcgggtc aggccgtctt cgccgacaac
     2881 agcacttgcg cgatcggtca ccggtgctcg atgccttcgt gatccagcag ccattccttc
     2941 gtcgggatgc cgttaccgcc gctgtagccg gtgatgcgtc cgttggaggc cagcacgcgg
     3001 tggcagggga tgatgatcgg taccgggttg gcgccgaccg cgccgcccac ggctcgcccc
     3061 gccgtgggac gaccggttgc catgccgatc tccccgtaag acgcgacctc tccgaacggg
     3121 atgtcggtga gctcgcgcca caccgagcgc tggaagtccg tgccgcccaa cgtgaccggc
     3181 acatcgaatt cgcgtcgcgt gcccgcgaag tactcggaca gttgagcgat tgccgtgtcg
     3241 atgaccgccg tgctggcctc gggctggccg tcgtggggca ggtggccgtc gcgctcgatg
     3301 gcgagcgagg tgatcgaggt gccgtcgctg gtgagctcga tgcgcccgat gggggagtcg
     3361 acgcgggcga tgtggatcga cggcgggggc ggcgagtcga tggtggtgtg gttctgcatg
     3421 ctttgaccct agccgcgtca tccgacggcc cactggcact tttcggacat gggagaaaac
     3481 gtggcttggg cgccgctggg ggaggatggg agcatgatca ctctccctca tcgtgaactc
     3541 ggccgctcgg gtcttcaggt ctcggtcgtg gggctcggtt gcaacaactt cggccgaccg
     3601 ggcagtcgca ccgcgagcca ggagggcacg acggcagtca tcgacgcggc tgacgatctg
     3661 ggcgtcaccc tgctcgacac cgcagacatc tacggcggtc atcccggtca gagtgagacc
     3721 tatctcgggg tcgccttggc cg
//
LOCUS       DAOSOU010000207         3737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_24683_length_3737_cov_5.437262, whole genome shotgun sequence.
ACCESSION   DAOSOU010000207 DAOSOU010000000
VERSION     DAOSOU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24683_length_3737_cov_5.437262"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /gene="dinB"
                     /locus_tag="PLW15_09985"
     CDS             complement(<1..951)
                     /gene="dinB"
                     /locus_tag="PLW15_09985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT34761.1"
                     /translation="MGKQDGRDRQVTVGPIDDSTATILHVDMDAFFASVELLDHPELV
                     GKPVIVGHNSSRSIVTAATYEARKFGINSALPMSIALKRCPQAIILEPHFDRYHHFSK
                     QVFAICDELTPSVERLGIDEAFLDVSGAGRVIGSPVQVAALLRQRVRAETGLVCSVGA
                     AATKFVAKVASGMSKPDGLLVIPREQTTAFLHPLPIAKLWGVGGKTEQVLRGRGIHTI
                     GALAATPVDVLARYLGDAAGSRLHDLAWGIDPRVVHAGADEKSVGHEMTFETDITDPV
                     IIHRELLRMSSMVAVRLRKAGMRGRTVVLKLRYEDFTTITR"
     gene            complement(1040..1297)
                     /locus_tag="PLW15_09990"
     CDS             complement(1040..1297)
                     /locus_tag="PLW15_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOT34762.1"
                     /translation="MGWFSFLILGIIAGVIAKLILRQKAGWFLTIILGVLGAMVGGWI
                     AGLIPGIGYENFWSIQSWLIAIAGALVVLFVFGLITRRRRA"
     gene            1428..2189
                     /locus_tag="PLW15_09995"
     CDS             1428..2189
                     /locus_tag="PLW15_09995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT34763.1"
                     /translation="MAQQHPWTRYVAIGDSFTEGIGDPEPQSPGGHRGWADRVAEVLS
                     SQSTDFAYANLAIRGRLLQQISDEQVDSALALNPDLITVSAGGNDIIRPGTDPDDVAQ
                     RFEDMVVRLRGDGATVVMFNGPDIGMTPVLRRVRGKVAIYNENLRAIAQRHDAIVADM
                     WALRELGDPRMWAPDRLHFSPIGHHTIARMVLDALNVENELEPLKPEPMPQRSWRQAR
                     VEDAVWAREHFVPWVLRRVRGRSSGDEVRPKRPEF"
     gene            complement(2191..3237)
                     /locus_tag="PLW15_10000"
     CDS             complement(2191..3237)
                     /locus_tag="PLW15_10000"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOT34764.1"
                     /translation="MPNPLSEFSALLHTIRDAGLLRRRRTFYFVTFGVITIAMVAAWF
                     GFAMLQDTWYTLLIAGALGILFTQYAFITHELAHRQVFESGKLNEFSGRIMADLVVGI
                     SYSWWMSKHSRHHANPNTVDKDPDIETDFIIFQKDHATGLKGFAGFFAKRQGWLFFPV
                     LLAEGLNLHLHAFKTVFSPGKVDKRALEIVLLTVRNVGYLAVVFSFLPLGMAFAFVGV
                     QLAIFGLYMGASFAPNHKGMPILPRGSKVDFLRRQVLTSRNIRSGHVMNHFMGGLNYQ
                     IEHHLFPNMPRPSLKRAGEIVREYCESRGILYTEVGLFTSYGIVIRHLNEVGLGADGD
                     PFDCPAAGRTGYAG"
     gene            complement(3410..>3737)
                     /locus_tag="PLW15_10005"
     CDS             complement(3410..>3737)
                     /locus_tag="PLW15_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOT34765.1"
                     /translation="RGLLAQADAGFREVTLTTEGDAFAQYDTPWGDTAEAVAAQTTTA
                     VVWSNAPVSVDVEVDPVRLAPAGTGVGTAVFAFGDQRVSVDLDLDATIDDPGPWWRLT
                     NPTRLF"
BASE COUNT          676 a         1182 c         1276 g          603 t
ORIGIN      
        1 ccgggtgatc gtcgtgaagt cctcgtagcg cagcttgagc accaccgtgc ggcctcgcat
       61 gcccgccttg cgcaggcgca cagccaccat gctcgacatc cgcaggagct cgcggtggat
      121 gatgacgggg tcggtgatgt cggtttcgaa ggtcatctcg tgcccgacgc tcttctcgtc
      181 ggcgcccgcg tgcaccaccc gggggtcgat gccccaggcg agatcgtgca ggcgagagcc
      241 tgcggcatcc cccagatagc gcgcaagcac gtcgaccggg gtcgcggcga gggcaccgat
      301 ggtgtggatg ccgcggccgc gcagcacctg ctcggtcttg ccgcccacac cccagagctt
      361 ggcgatggga agggggtgca ggaacgctgt cgtctgctcg cgagggatca cgagcagtcc
      421 gtcgggcttg gacattccgc tggcgacctt cgcgacgaac ttggtcgcgg cggcacccac
      481 cgaacagacc aggccggtct cggcgcgcac ccgctgcctc agcagggccg cgacctggac
      541 cggcgagccg atcacgcggc cggccccaga cacatcgagg aacgcctcat cgattcccag
      601 ccgctcgacc gagggcgtga gttcgtcgca gatcgcgaag acctgcttcg agaagtggtg
      661 gtaacggtcg aagtgcggct cgaggatgat cgcctgggga caccgtttca aggcgatcga
      721 catgggcagt gccgagttga tgccgaactt gcgtgcctcg tacgttgccg ctgtgacgat
      781 cgatctcgag gagttgtgcc cgacgatcac gggcttgccc acgagctcgg ggtgatccag
      841 cagctcgact gacgcgaaga aggcatccat gtcgacgtgg aggatcgtcg cggtgctgtc
      901 gtcgatggga ccgacagtga cctgacggtc gcgcccatcc tgcttgccca tggtcacatc
      961 atcgcaccgg catccgacgc gggggcgaac gcagaagcgg gcaccgaccg tggccggtgc
     1021 ccgcctcagt gctgtcgaat caggctctgc gacgtcgcgt gataagtccg aagacgaaca
     1081 gaacgaccag cgcgcccgcg atggcgatga gccacgactg gatcgaccag aagttctcgt
     1141 aaccgatgcc ggggatgagt cccgcgatcc agccaccgac catggcaccc aggacgccga
     1201 ggatgatcgt gaggaaccat cctgccttct ggcgcaggat cagcttggca atgacgccag
     1261 cgatgattcc gaggatgagg aaggaaaacc agcccattga tgacgctcca tgtgttgtgg
     1321 cggtgcgagt ctcacaccgc acgctcagct tggcactgcg cccagcgctc gacaagcagc
     1381 aacacgcgag gtggcgagcg tgccgctggc tgagcaatgc tagtggcatg gcacagcagc
     1441 atccgtggac caggtatgtc gcgattgggg actctttcac cgagggcatc ggcgaccctg
     1501 aaccgcagag tcccggcgga catcgcgggt gggcggaccg cgtggccgag gtgctcagtt
     1561 cgcagagcac cgacttcgcc tatgcgaatc tcgcaatccg gggacgactt ctccagcaga
     1621 tctccgacga gcaggtcgat tcagccctcg cgctcaatcc cgacctcatc acggtctccg
     1681 cgggcggcaa cgacatcatc cgcccgggca ccgatccgga tgacgtcgcc cagcgtttcg
     1741 aggacatggt cgtgcgcctg cgcggtgacg gcgcgaccgt ggtgatgttc aatggtcccg
     1801 acatcgggat gacaccggtg ctgcgtcggg tgcgcggcaa ggtggcgatc tacaacgaga
     1861 atctgcgggc gatcgcgcag cgccacgacg cgatcgtcgc cgacatgtgg gccctgcggg
     1921 agcttggcga ccctcgcatg tgggcgcccg accgcctgca cttctccccc atcggccacc
     1981 acacgatcgc gcggatggtg ctcgacgccc tcaacgtgga gaacgagctg gagccgctca
     2041 agcccgagcc gatgccacaa cggtcgtggc ggcaggcccg ggtcgaggac gccgtctggg
     2101 ctcgtgagca cttcgtgccc tgggtgctgc gtcgcgtgcg cggcaggtca tccggcgacg
     2161 aggtgcgacc gaagcgcccc gagttctagg tcagccggcg tacccggtgc gaccggcggc
     2221 cgggcagtcg aacgggtcgc cgtcggcacc cagtccgacc tcgttgaggt gacggatgac
     2281 gatgccgtag gacgtgaaca gccccacctc ggtgtagagg atgccgcgac tctcgcagta
     2341 ctcgcgcacg atctcacccg cccgcttgag gctgggtcgc ggcatgttcg ggaacaggtg
     2401 gtgctcgatc tggtagttga gcccgcccat gaagtggttc atcacgtggc cgctgcggat
     2461 gttgcggctg gtgagcacct ggcggcgaag gaagtcgacc ttcgacccgc gcggcaggat
     2521 cggcatgccc ttgtggttcg gcgcgaacga tgcccccatg tagaggccga agatggccag
     2581 ctgaacgccg acgaacgcga aggccatgcc gagcggcagg aagctgaaga cgacggcgag
     2641 gtagccgacg ttgcgcactg tcagcagcac gatctccaga gcgcgcttgt cgaccttgcc
     2701 gggcgagaag accgtcttga acgcgtggag gtgaaggttc agaccctcgg ccaggagcac
     2761 ggggaagaac agccacccct gacgcttggc gaagaagccc gcgaagccct tcaggcccgt
     2821 cgcgtggtcc ttctggaaga tgatgaagtc cgtctcgatg tccggatcct tgtcgacagt
     2881 gttggggttc gcgtgatggc gggagtgctt gctcatccac caggagtagc tgatgcccac
     2941 gacgaggtcg gccatgatcc ggccggagaa ttcgttgagc ttgcccgatt cgaacacctg
     3001 tcggtgcgcg agctcgtggg tgatgaacgc gtactgggtg aagaggatcc cgagcgcgcc
     3061 cgcgatcagc agcgtgtacc aggtgtcctg aagcatcgcg aagccgaacc acgcggcaac
     3121 catggcgatc gtgatgacgc cgaaggtgac gaagtagaag gtccggcggc ggcgcagcag
     3181 gccggcatcc cgaatcgtgt gcagcagtgc ggagaactcg ctcagcgggt taggcacgcc
     3241 gcgagggcca gtcttgatga cgcgtacggg ttcgccggct actgcagaag tcacgtggcc
     3301 tctaaagggt cgaatgactt cacagccaac aaagtgcgag cagatgctca gatgtcttga
     3361 gcctagcggc cgagactggg aatcaccggc atgttgacac ccagtacgac tagaacagcc
     3421 gagtcgggtt ggtgagtcgc caccaggggc cgggatcgtc gatggtcgcg tccagatcga
     3481 ggtcgacgct cacccgctgg tcgccgaagg cgaacactgc cgtgcccacg ccagttcccg
     3541 cgggagcgag tcgcaccggg tccacctcga cgtcgacgct taccggcgcg ttcgaccaca
     3601 cgacggcggt cgtggtctgc gccgccaccg cttcggcagt gtcgccccac ggcgtgtcgt
     3661 actgcgcgaa ggcgtctccc tcggtggtca gcgtgacctc ccggaatccg gcgtcggcct
     3721 gggcgagaag tccgcga
//
LOCUS       DAOSOU010000208         3705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_24940_length_3705_cov_6.266301, whole genome shotgun sequence.
ACCESSION   DAOSOU010000208 DAOSOU010000000
VERSION     DAOSOU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24940_length_3705_cov_6.266301"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLW15_10010"
     CDS             complement(<1..701)
                     /locus_tag="PLW15_10010"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOT34766.1"
                     /translation="MPAIVIIGAQWGDEGKGKATDLLADRIDYVVKFNGGNNAGHTVV
                     IGDQKYALHLLPSGILTDGVIPVIANGVVIDIEVLFDELTALASRGVDVSKLVVSANA
                     HVITQYHRTLDKVTERFLGKRQIGTTGRGIGPAYADKINRVGIRIQDLFDENILRQKV
                     EGALHQKNHLLVKIYNRRAIAVDEIVDDLLSYADRLRPMVADTGLLLNQALDAGKTVV
                     FEGGQATMLDVDHGT"
     gene            complement(839..1486)
                     /locus_tag="PLW15_10015"
     CDS             complement(839..1486)
                     /locus_tag="PLW15_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOT34767.1"
                     /translation="MTDEPNPSHELTTHGVGPWRGEWPTEAHYDPELLAKGDTRNVID
                     RYRYWSMEAIVADLDTRRHPFHVAIENWQHDMNIGSIVRSANAFLADTVHIIGRRRWN
                     KRGAMVTDRYQHVLNHPDVATFVSWAREHDLPIVAVDNLPGSVRIESTTLPERCVLLF
                     GQEGPGLSDEALAAADLSVEISQFGSTRSINASAAAAIVMHVWVTQHGLDKLDHR"
     gene            1720..2562
                     /locus_tag="PLW15_10020"
     CDS             1720..2562
                     /locus_tag="PLW15_10020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOT34768.1"
                     /translation="MPWTPDGPNLVIAGDNLTVTATLPDESFRLVYLDPPFNTGRTQR
                     RQTITTKQSADGARSGFKGRSYETIKGTLTQYDDRFADYWEFLEPRLMEAWRLLDDRG
                     TLYLHLDYREAHYAKVVLDALFGRDNFLNELIWAYDYGARATRKWPAKHDTILVYVKN
                     PKSYHFDNAAVDREPYMAPGLVTPEKVERGKLPTDVWWHTIVSPTGREKTGYATQKPE
                     GILRRIIQASSAPDDWVLDFFAGSGTTGAVAASLGRRFVLVDENPDALATMRARLGEG
                     VVFE"
     gene            2562..3494
                     /locus_tag="PLW15_10025"
     CDS             2562..3494
                     /locus_tag="PLW15_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOT34769.1"
                     /translation="MHLRVISPAETTEEVLSYLVAEPAVANLVVHRGAAVDPAGDVVI
                     ADVARESANEVLRALKAMGLDKNGSIALDAVGVALSSRGSEAEKAAPGAGDDAVVWEE
                     IEQRTGEESRLSVTFLLFLSIATVIAGIGVLLDQPILIVGAMVVGPEFGPLAALSVGV
                     IRRRPRVIGRSLLALTVGFAVAIVVTVLSTLALNAGGLVTREMLLGARPLTDFIWRPD
                     ALSWVIAFLAGIAGILSLTTAKSGALIGVLISVTTVPAAGNVAVAIAYGVPDEAWGSA
                     LQLGINLGAIATAGILTLAVQRWLSRRRRPAARA"
     gene            3536..>3705
                     /locus_tag="PLW15_10030"
     CDS             3536..>3705
                     /locus_tag="PLW15_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HOT34770.1"
                     /translation="MSKRADVECWLTDMDGVLVHENRPIPGASALLQQWRDQDKPFLV
                     LTNNSIFTPRDL"
BASE COUNT          566 a         1236 c         1276 g          627 t
ORIGIN      
        1 tacgtgccgt ggtcgacatc cagcatcgtg gcctgtccac cctcgaagac gacggtcttg
       61 ccggcgtcga gggcctggtt caggagcagg ccggtgtcag ccaccatcgg acgcaggcgg
      121 tctgcgtagc tgagcaggtc atccacgatc tcgtcgacgg cgatggcacg acggttgtag
      181 atcttcacca gcaggtggtt cttctggtgc agtgcaccct ccaccttctg gcgcaggatg
      241 ttctcgtcga acaggtcctg gatgcggatg cccacccggt tgatcttgtc ggcgtaggcc
      301 ggaccgatgc cgcgacccgt cgtgccgatc tgacgcttgc cgaggaaccg ctccgtcacc
      361 ttgtcgagcg tgcggtggta ctgcgtgatc acgtgggcgt tggcgctcac cacgagcttc
      421 gagacatcca cgccgcggct ggcgagggcg gtcagctcgt cgaacagcac ctcgatgtcg
      481 atgacgacgc cgttggcgat caccgggatg acgccgtcgg tgagaattcc ggagggcagc
      541 aggtgcagcg cgtacttctg gtcgccgatc acgacggtgt gtccggcgtt gttgccaccg
      601 ttgaacttga ccacgtagtc gatgcggtcg gcgagcaggt cggtggcctt gcccttgccc
      661 tcgtcacccc actgggcgcc gatgatcacg atggctggca tgggctatct ccctgataga
      721 ggtggtgggt ggggccacgc cgccggttct ggaccgacgc atcgcggcgg gccagccgtg
      781 cggtggggat tacgccccag tctatcggtg gcgcctcggt ggtcgagcct gtcgagacct
      841 atcggtggtc gagcttgtcg agaccgtgct gcgtcaccca cacgtgcatc acgatcgcgg
      901 cggcggccga ggcattgatg ctgcgcgtcg acccgaactg cgagatctcg accgagaggt
      961 cggcggcggc gagggcctcg tcgctgagcc ccggaccctc ctggccgaag agcagcacgc
     1021 agcgctccgg cagagtggtc gactcgatcc gcacgctgcc cggcagattg tcgacggcga
     1081 cgatgggcag gtcatgttcg cgggcccagg agacgaaggt cgcgacatcc ggatgattca
     1141 gcacgtgctg atagcggtcg gtgaccatgg cgccccgctt gttccaccgc cgccgaccga
     1201 tgatgtgcac ggtgtcggcg aggaacgcgt tggcgctgcg cacgatcgag ccgatgttca
     1261 tgtcgtgctg ccagttctcg atggcgacgt ggaacggatg ccgccgggtg tccaggtcgg
     1321 cgacgatcgc ctccatgctc cagtagcggt agcggtcgat cacgttgcgg gtgtcgccct
     1381 tggccagcaa ctccgggtcg tagtgcgcct cggtgggcca ttccccccgc cacgggccga
     1441 cgccgtgcgt cgtgagctcg tggctcgggt tcggctcgtc ggtcacgggt acgagcgtac
     1501 ggcttcggcg ccccccaacc cccgatgttc gtaactcctg cacggttccc gcggtgccgc
     1561 ggcagtgcgg cggtattccg cgagagggga tcgcgtcgac cggaggattg caggagttat
     1621 cgacacaggg tgacggtggg ctcgggcggc acggggctcg gggcgccgag cgagcgcgtc
     1681 gagtccgctg cggccacgtg cggctgttaa tctcgcccca tgccctggac gcccgacgga
     1741 cccaacctgg tgatcgccgg cgacaacctc acggtgacgg cgaccctgcc cgacgagagc
     1801 ttccggctgg tctacctcga tcctccgttc aacaccgggc gcacgcagcg gcgtcagacg
     1861 atcaccacca agcagagcgc ggatggcgcg cgctcgggtt tcaagggccg cagctacgag
     1921 accatcaagg gcaccctcac ccagtacgac gaccggttcg cggactactg ggagttcctc
     1981 gagccccgcc tcatggaggc gtggcggctg ctcgacgacc gcggcacgct gtacctgcac
     2041 ctcgactacc gcgaggcgca ctacgcgaag gtcgtgctcg acgccctgtt cggccgcgac
     2101 aactttctca acgagctcat ctgggcctac gactacggcg cccgcgcgac tcgcaagtgg
     2161 ccggccaagc acgacacgat cctcgtctac gtcaagaacc cgaagagcta ccacttcgac
     2221 aacgcggccg tcgatcgcga gccgtacatg gcgcccgggc tcgtcacacc ggagaaggtc
     2281 gagcgcggca agctgcccac cgacgtctgg tggcacacca tcgtgtcacc caccggcagg
     2341 gagaagacgg ggtacgcgac gcagaagccc gagggaatcc tgcgccgcat catccaggcg
     2401 tcgagtgcac cggatgactg ggtactcgac ttcttcgccg gcagcggcac gaccggcgca
     2461 gtcgcggcga gcctcggccg tcgattcgtg ctcgtcgacg agaatccgga tgcgctggca
     2521 accatgcgcg cgcgactcgg tgagggggtg gtgttcgagt gatgcacctc agggtgatca
     2581 gccccgccga gacgaccgag gaagtgctct cgtacctggt cgctgagcca gccgtcgcga
     2641 acctcgtcgt gcatcgcggt gccgccgtcg acccggccgg cgacgtcgtg atcgcggatg
     2701 tcgcccgcga gtcagccaac gaggtgctgc gcgcgctcaa ggcaatgggc ctcgacaaga
     2761 acgggtcgat cgccctcgac gcagtgggcg tcgccctctc cagccgaggc agcgaggcgg
     2821 agaaggcggc accgggcgcc ggcgacgacg ccgtcgtctg ggaggagatc gagcagcgca
     2881 ccggcgagga gtcacggctc tcggtcacgt tcctgctgtt cctgtcgatc gcgaccgtca
     2941 tcgccggcat cggcgtgctg ctcgatcagc cgattctgat cgtcggggcc atggtggtcg
     3001 gccccgagtt cgggccactc gcggccctgt cggtcggcgt gatccggcgc cgcccgcggg
     3061 tgatcgggcg ctcgctgctc gcgcttacgg tcggattcgc tgtcgcgatc gtcgtgaccg
     3121 tgctgtcgac cctcgcactc aacgcgggcg ggctcgtcac acgcgagatg ctcctcggtg
     3181 cgcgcccgct caccgacttc atctggcgac cggatgccct gtcctgggtc atcgcgttcc
     3241 tcgcgggtat cgcgggcatc ctgtcgctga cgaccgccaa gtcgggcgcg ctgatcggtg
     3301 tgctcatctc cgtgaccact gtcccggccg ccggtaacgt ggcggtcgcg atcgcgtacg
     3361 gcgttcccga tgaggcgtgg ggatcagcac ttcagctggg gatcaatctc ggcgcgatcg
     3421 cgacggcggg catcctgacg ctggcggtgc agcggtggtt gtcgcgcagg cggcgcccgg
     3481 ccgcacgcgc ctaggtctcg actcgctcga ccaccggggc cggtaccatg gcggcatgag
     3541 caagcgagcg gatgtcgagt gctggctgac cgacatggac ggagttctcg tccacgagaa
     3601 ccggccgatc cccggggcga gcgccctgct gcagcagtgg cgcgaccagg acaagccctt
     3661 cctcgtgctc accaacaact cgatcttcac gccacgcgac ctgag
//
LOCUS       DAOSOU010000209         3695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_25036_length_3695_cov_4.500275, whole genome shotgun sequence.
ACCESSION   DAOSOU010000209 DAOSOU010000000
VERSION     DAOSOU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25036_length_3695_cov_4.500275"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLW15_10035"
     CDS             complement(<1..416)
                     /locus_tag="PLW15_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT34771.1"
                     /translation="MAKARGEKTLGWRILAGIVIPILLVLGRYTFRNSERVPRTGPVI
                     ITPNHYSNIDPLVSAYAVWRTGRVTRFLAKASLFKVPVFGAILRGTGQIPVQRAGANA
                     GRHWLVRGREPGRWAGLEPGHGRRERQRTARLRRSQR"
     gene            complement(423..1790)
                     /gene="murA"
                     /locus_tag="PLW15_10040"
     CDS             complement(423..1790)
                     /gene="murA"
                     /locus_tag="PLW15_10040"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT34772.1"
                     /translation="MNSLTKDAQKAAARVGLTSDKIVINGGKPLRGRVDVRGAKNLAT
                     KAMVAALLADTPSLLKDVPDISDVGVVSRMLEAYGVSVTKVGEGELMLDPTNVLKAQF
                     HEIDALAGSSRIPILFCGPLLHRLGEALIPDLGGCRIGDRPIDFHLNALRAFGAVVDK
                     SYEGIRLTAPNGLKGANIELPYPSVGATEQVLLTAVLAEGVTELKNAAIEPEIMDLIA
                     ILQKMGAIINVEPNRVIFIEGVEKLSGYEHRALFDRNEAASWASAALATGGDIFVVGA
                     KQQELMTFLNIFRKVGGAFDIHEDGIRFYHPGGTLKPVVIETDVHPGFMTDWQQPLIV
                     ALTQAEGRSVVHETVYENRFGFTDALIEMGANITVHQDGLESISRRVPRRPLEQAAVI
                     IGPTPLHGADVEIPDLRGGFSHLIAALTADGQSTVTNIGIISRGYEHFIDKLRVLGAD
                     FVYEG"
     gene            complement(1861..2472)
                     /gene="leuD"
                     /locus_tag="PLW15_10045"
     CDS             complement(1861..2472)
                     /gene="leuD"
                     /locus_tag="PLW15_10045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOT34773.1"
                     /translation="MDKISTVTGIAVPLERSNVDTDQIIPAEFLKRVTKTGFDDALFY
                     AWRQDPDFVLNREPFAGGPGHGTRILVAGPDFGTGSSREHAVWALRDYGFQAVLSARF
                     GDIFRGNSGKQGLLAAQLAEKDIETIWMIVAEHPGIEVSVDLVARTVTVGKASFVFEI
                     DDYTRWRLLEGLDDIGLTLRDESQITRFEAKRESWRPTTLPAR"
     gene            complement(2472..>3695)
                     /gene="leuC"
                     /locus_tag="PLW15_10050"
     CDS             complement(2472..>3695)
                     /gene="leuC"
                     /locus_tag="PLW15_10050"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOT34774.1"
                     /translation="ANAKEFGIRLHSLGDIEQGIVHVVGPQLGLTMPGITVVCGDSHT
                     STHGAFGAMAFGIGTSEVEHVMATQTLPLKPFKTMAITVEGTLKPGVSAKDIILAVIA
                     KIGTGGGQGYVLEFRGSAIRALSMEGRMTICNMSIEAGARAGMVAPDQTTYDYLKGRP
                     HAPSGADWDAAVAYWNTLATDDDAVFDAEVFLDANELEPFVTWGTNPGQGVSLSETVP
                     NPASYDDPNERAAAERALEYMDLTAGTPMKDIRVDAVFMGSCTNSRIEDLRAFASVIK
                     GKKKADHVRVMVVPGSARVRIEAEAEGIDKIVEEFGAEWRFAGCSMCLGMNPDQLAPG
                     ERCASTSNRNFEGRQGKGGRTHLVSPLVAAATAIRGTLSSPADVASVSTSSTTASSTT
                     EGSTTEGSTTEGVTA"
BASE COUNT          581 a         1198 c         1261 g          655 t
ORIGIN      
        1 cgttgggatc ggcgcagccg ggcagtccgc tggcgctccc gccgcccgtg cccaggctca
       61 agcccagccc accgtccagg ctcacgcccc ctgacgagcc agtggcgtcc ggcgttggcc
      121 ccggcacgct gcaccgggat ctgccctgtg ccgcgcagga tggccccgaa caccggaacc
      181 ttgaacaggc tggccttggc gaggaacctg gtcacccggc cggttctcca gacggcgtag
      241 gccgagacca gcgggtcgat gttggagtag tggttcggtg tgatgatcac cgggccggtg
      301 cgcgggacgc gctcagagtt gcggaaggtg tagcggccga gcacgaggag aatcgggatg
      361 acgataccgg cgaggatccg ccagccgagg gtcttctcgc cccgtgcctt cgccatcgcc
      421 gcttagccct cgtagacgaa gtcagcgccg agcacccgca gcttgtcgat gaagtgctcg
      481 tagccgcggc tgatgattcc gatgttggtg acggtcgact gaccgtcggc ggtgagtgcc
      541 gcgatgaggt ggctgaagcc accgcgcagg tcgggaatct ccacgtcggc gccgtgcagc
      601 ggggtcgggc cgatgatcac agccgcctgc tcgagcgggc gacgcggcac acgacggctg
      661 atggactcca gcccgtcctg gtgcaccgtg atgttcgcgc ccatctcgat gagcgcatcc
      721 gtgaagccga agcggttctc gtacaccgtc tcgtgcacca ccgaacgacc ctccgcctgg
      781 gtgagcgcga cgatgagcgg ctgctgccag tcggtcatga aaccgggatg cacgtcggtt
      841 tcgatgacga ccggcttgag cgtgccgccc gggtggtaga agcggatgcc gtcctcgtga
      901 atgtcgaacg cgccacccac cttgcggaag atgttgagga acgtcatcag ctcctgctgc
      961 ttcgcgccca cgacgaagat gtcgccgccg gtcgccagtg ccgcggatgc ccagctcgcg
     1021 gcctcgttgc ggtcgaagag tgcgcggtgc tcgtagccgc tgagcttctc gacaccctcg
     1081 atgaagatca cgcggttggg ctcgacgttg atgatcgcgc ccatcttctg gaggatcgcg
     1141 atgaggtcca tgatctcggg ctcgatggcc gcgttcttga gctcggtcac gccttcggcg
     1201 agcaccgccg tgagcagtac ctgctcggtc gcgccgacgc tcgggtaggg cagctcgatg
     1261 ttggcaccct tgagcccgtt gggggccgtc aggcggatgc cctcgtagct cttgtcgacg
     1321 acggcaccga aggcgcgcag cgcgttgagg tggaagtcga tggggcggtc gccgatgcgg
     1381 cagccaccga gatcggggat cagcgcctca ccgaggcggt gcagcaacgg cccgcagaac
     1441 aggatcggga tgcggctgga cccagccagc gcgtcgatct cgtggaactg ggccttcagc
     1501 acgttggtgg ggtcgagcat gagctcgccc tcgccgacct tggtcaccga gacgccgtac
     1561 gcctcgagca tccggctgac gacgccgacg tcactgatgt cgggaacgtc cttgagaagg
     1621 ctgggcgtgt cggccagaag ggcagcgacc atggccttcg ttgcgaggtt cttggccccg
     1681 cgcacatcca cgcgaccgcg caacggcttg ccgccgttga tcacgatctt gtcggacgtg
     1741 agtcccactc tcgccgcagc cttttgggca tccttggtaa gagaattcat gctcacctct
     1801 gtcttggttg gtgctggcgc tgtggtggtg cgccccgtag tcaggctaac gagcgagaag
     1861 ctaacgagcc ggaagagtcg tgggccgcca gctttcgcgt ttggcctcga atcgcgtgat
     1921 ctgggactcg tcgcgcaacg ttaagccgat gtcatcgagt ccttcaagca aacgccacct
     1981 agtgtaatcg tcaatctcga acacgaagct ggccttgcct acggtcacag ttcgtgcaac
     2041 gaggtcgacc gagacctcta ttcccggatg ctccgccacg atcatccaga tcgtctcgat
     2101 gtccttctcc gcgagttggg cagcgagcag accctgtttg ccggagttgc cgcggaagat
     2161 gtcgccgaac cgcgcgctca gaacggcctg gaagccgtag tcgcgcagcg cccacacggc
     2221 gtgctcgcga ctcgagccgg tgccgaagtc gggaccagcg acgagaatgc gcgtgccgtg
     2281 gccgggacca ccggcgaacg gctcacggtt gagcacgaag tcgggatcct ggcgccaggc
     2341 gtagaacagg gcgtcatcga agccggtctt ggtgactcgc ttgaggaact cggcggggat
     2401 gatctggtcg gtgtcgacgt tcgagcgctc cagcggcacc gcgattccgg tgactgtcga
     2461 gatcttgtcc attaagcggt caccccttcg gtggtcgagc cttcggtggt cgagccttcg
     2521 gtggtcgagc tcgcggtggt cgagcttgtc gagaccgacg cgacatccgc cgggctcgac
     2581 agcgtgccgc gaatcgcggt cgccgcggcc accaacgggc tcaccaggtg ggtgcgcccg
     2641 cccttgccct gccgaccctc gaagttgcga ttcgaggtgg atgcgcagcg ctcccccggc
     2701 gcgagctggt cggggttcat gcccagacac atcgagcagc cggcgaagcg ccactcggcg
     2761 ccgaactcct cgacgatctt gtcgatgccc tccgcctcgg cctcgatgcg cacgcgcgcc
     2821 gagccgggaa ccaccatgac gcgcacgtgg tcggccttct tcttgccctt gatgacgctc
     2881 gcgaacgcgc gcaggtcctc gatgcggctg ttggtgcacg aacccatgaa cacggcatcc
     2941 acgcggatgt ccttcatcgg ggtgcccgcc gtcaggtcca tgtattcgag ggcgcgctcg
     3001 gcggcggcgc gctcgttggg gtcgtcgtag ctcgccgggt tgggaactgt ctcgctcagc
     3061 gagacgccct ggccggggtt ggtgccccac gtgacgaacg gctccagctc gttggcgtcg
     3121 aggaacacct cggcatcgaa gaccgcgtca tcgtcggtgg cgagcgtgtt ccagtaggcg
     3181 acggcggcat cccagtcggc tccggatggc gcgtgagggc gacccttcag gtagtcgtag
     3241 gtggtctggt cgggcgcgac catgccggcc cgcgctcccg cttcgatcga catgttgcag
     3301 atcgtcatgc ggccctccat ggagagcgcg cggatggcgc tgccgcggaa ctcgagcacg
     3361 tagccctgcc cgccgccggt gccgatcttg gcgatgacgg cgaggatgat gtccttcgca
     3421 ctcacgccgg gcttcagcgt gccctcgaca gtgatggcca tggtcttgaa gggcttgagc
     3481 ggaagcgtct gcgtggccat cacgtgctcg acctcgctcg tgccgatgcc gaacgccatc
     3541 gcgccgaacg cgccgtgggt ggaggtgtgc gagtcaccgc agaccacggt gatgcccggc
     3601 atcgtcagcc cgagctgcgg accgaccaca tgcacgatgc cctgctcgat gtcgccgagc
     3661 gagtgcaggc ggatgccgaa ctccttcgcg ttggc
//
LOCUS       DAOSOU010000210         3680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_25185_length_3680_cov_5.709517, whole genome shotgun sequence.
ACCESSION   DAOSOU010000210 DAOSOU010000000
VERSION     DAOSOU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25185_length_3680_cov_5.709517"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLW15_10055"
     CDS             complement(<1..426)
                     /locus_tag="PLW15_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT34775.1"
                     /translation="MLPAPKPDGLSLADVLESCLSAIQGQGNRLGLPPVERAVVLLVD
                     GLGAEALKATAGHARTLSGALTTKSVIEAGFPTTTAAALASLTTGQLPGQHGLVGYSV
                     LDSAHDRVVNQLSGWDQNLDPTTWQLQPTILERASAAGFG"
     gene            439..2907
                     /locus_tag="PLW15_10060"
     CDS             439..2907
                     /locus_tag="PLW15_10060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOT34776.1"
                     /translation="MATPENPSAVTPSADSRTGERIEDVDVSAEMQGSFLEYAYSVIY
                     SRALPDARDGLKPVQRRILYQMTEMGLRPDRGHVKSSRVVGDVMGKLHPHGDASIYDA
                     LVRLTQDFVLRVPLIDGHGNFGTLDDGPAAPRYTEARLQAAALAMTDGLDEDVVDFVP
                     NYDNSLTQPEVLPSAFPNLLVNGASGIAVGMATNMAPHNLIEVVGAARHLLDHPDATL
                     DDLMSFVPGPDLPTGGTIIGLEGIRDAYATGRGAFKTRAKVSVEAITARKTGLVVTEL
                     PYLIGTERVIEKIKDGVNSKRINGISDVTDLTDRKHGMRLVIGIKTGFSPDAVLEQLY
                     RLTPLEDSFSINNVALVGGGPRTLGLKELLQVYLDHRLSVVRRRSEYRLARRRERLHL
                     VEGLLIAILDIDEVIQVIRQSDDSDQARARLIDVFDLSELQAEYILELRLRRLTKFSR
                     IELEAEADKLRAEIAYLEGVLADPARIRQLVSEELEQVAERFGTPRRTLLTEARASVA
                     TTSAARKAAPVLEIADAPCEVLLSTTGRAIRIDLPEDGSPLAPARRRSRHDAIRSRVT
                     TTSRSEIGAVTSTGRLIRLTAVDLPSVPANSIQLAAGAKITEYLAIGSKERVLGLVTL
                     DGEPIALGTRQGTVKRVAPGSWPSKPEFEIISLKPGDEVVGVSQGGDGDELVFVTSDA
                     QLLHFGASAVRPQGPSAGGMAGINLSAKATVTFFGSVDPAADNVVVTLSTSVATIAGA
                     DPGRGKVSPLSEFPGKGRATGGVRAHSFLKGEDVLALAWVGAAPANAVGTDGSIRTLP
                     ETGAKRDASGTPLDGVVGSVGSSV"
     gene            complement(2915..>3680)
                     /locus_tag="PLW15_10065"
     CDS             complement(2915..>3680)
                     /locus_tag="PLW15_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT34777.1"
                     /translation="EDAGQLLMTAPVDQLVAATTKMQLRAPDETPGTIALAPVIDGEF
                     VPERPLDAFKAGRAHRVPLIIGTNDREGSLFRGRLDILATTPPRIRAIFAKTRKRSRK
                     AVKAQYPGLPAPRPAADFAGDYSFWYPSVKVAERHARFAPVYFYRFDIAPRLLRWAGL
                     DATHGLELFALFDRMDGLFGRGIGAAGGRAAFKRAGVRMQRHWLSFARTGEVGSDWPR
                     YTAKKRRTLIIDRTDRVEEDPRRARRVAWQEFVPHI"
BASE COUNT          612 a         1272 c         1225 g          571 t
ORIGIN      
        1 accgaagccc gccgcggaag cccgctcgag gatggtgggt tggagctgcc aggtcgtggg
       61 atcgagattc tggtcccacc cggagagttg attgacgact cggtcgtggg cgctgtcgag
      121 cacgctgtag ccaacgagtc cgtgctggcc gggcagctgc ccggtggtga gcgaggcgag
      181 cgccgcggcg gtggtcgtcg ggaagccggc ctcgatcacc gacttggtcg tgagggcgcc
      241 cgagagcgtc cgggcgtgtc cggccgtcgc cttcagcgcc tcggcaccga gaccatcgac
      301 gagcagcacc acggcgcgtt cgacgggcgg caaacccagc cgattgccct gtccctgaat
      361 cgcgctgaga caactttcca gcacatcggc aaggctcaac ccgtctggtt tgggcgccgg
      421 taacatgggg gcaatcctat ggctacccca gaaaatccct ccgccgtcac gccctccgcc
      481 gactccagaa ctggcgaacg catcgaggac gtcgatgtat cagccgagat gcagggctcg
      541 ttcctcgagt acgcctactc ggtcatctac tcccgtgcac tgcccgacgc ccgcgacggg
      601 ctcaagccgg tgcagcgtcg catcctgtac cagatgaccg agatgggcct tcggcccgac
      661 cggggccacg tgaagtcctc gcgggttgtc ggcgacgtca tgggcaagct gcacccgcac
      721 ggcgacgcct cgatctatga cgcactcgtg cggctcaccc aggacttcgt gctgcgcgtt
      781 ccgctcatcg acggccacgg caacttcgga accctcgacg atggaccggc ggcaccccgg
      841 tacaccgagg cccgactgca ggccgcagca ctcgccatga cggatggtct cgacgaagac
      901 gtcgtcgact tcgtaccgaa ctacgacaac tcactgaccc agcccgaggt gctgccgtcg
      961 gcgttcccca atctcctcgt caacggagcg agcggcatcg ccgtcggcat ggccacgaac
     1021 atggcaccgc acaatctcat cgaggtcgtc ggcgcggcgc gacacctcct ggaccacccc
     1081 gacgcgactc tcgacgacct catgtcgttc gtacccggcc ccgacctgcc gaccggtggc
     1141 acgatcatcg gtctcgaggg aatccgggat gcctatgcca ccggacgcgg ggcgttcaag
     1201 acgcgcgcaa aggtgtccgt cgaggcgatc acggcccgca agaccgggct cgtcgtcacg
     1261 gaacttccct acctcatcgg caccgagcgg gtgatcgaga agatcaagga cggcgtcaac
     1321 tccaagcgca tcaacggcat cagcgatgtc accgacctca ccgaccgcaa gcacggcatg
     1381 cgactggtga tcggcatcaa gaccggcttc agcccggatg ccgtactcga gcagctctac
     1441 cgcctcaccc ccctcgaaga cagcttctcg atcaacaacg tcgctctcgt cggtggtgga
     1501 ccgcgcacgc tgggcctgaa ggaactgctg caggtctacc tcgaccatcg cctgtcggtc
     1561 gttcgtcgcc gcagcgagta ccgactcgcg cgccggcgtg aacggcttca cctcgtcgag
     1621 ggtctgctga tcgcgatcct ggatattgac gaggtcattc aggtcattcg ccagagcgac
     1681 gacagcgatc aggcccgtgc gaggctcatc gatgtcttcg acctgagcga gctgcaggcc
     1741 gagtacatcc tggagctgcg actgcgccga ctgaccaagt tcagccggat cgagctggag
     1801 gctgaagccg acaagctacg cgccgagatc gcctacctcg agggcgtcct ggccgatccc
     1861 gcccgcatcc gtcagctcgt ctccgaagag ctcgagcagg tcgctgagcg attcggcaca
     1921 ccgcgacgca ccctgctcac cgaggcgcgc gcatccgtcg ccaccacctc ggcggcccgc
     1981 aaggcggcgc cggtgctgga gatcgcggat gcaccctgtg aggtgctgct gtcgaccacc
     2041 ggtcgcgcca tccgaatcga tctgcccgag gacggcagcc cgctcgcgcc cgcccgtcgt
     2101 cgcagcaggc acgatgcaat caggtcccgc gtgaccacga cgagtcgaag cgagatcggg
     2161 gccgtcacca gtaccgggcg cctcatccgt ctcaccgccg tcgacctgcc gagcgttccc
     2221 gcgaattcga tccagctcgc ggccggcgcc aagatcacgg aatacctggc gatcggctcc
     2281 aaggagcggg tgctcggtct cgtcacgctc gacggcgagc cgatcgctct cggaacccgg
     2341 cagggtaccg tcaagcgtgt cgcgcccggc tcgtggccga gcaagcccga gttcgagatc
     2401 atctcgctca agccgggcga cgaggtcgtc ggagtgtcgc agggaggcga cggcgatgag
     2461 ctcgtcttcg tgacctcgga tgcgcagctg ctgcacttcg gtgcgtcggc cgtgcggccg
     2521 caggggccgt cggccggtgg aatggcgggc atcaacttga gcgccaaggc gacggtcacc
     2581 ttcttcggct ccgtcgaccc ggccgccgac aacgtcgtgg tgaccctgtc gacgtcggtc
     2641 gccacgatcg ccggcgctga ccccgggcgc ggcaaggtgt cgccgctgtc cgagttcccc
     2701 gggaagggac gagcgaccgg cggagtgcga gcgcatagct tcctcaaggg cgaagacgtt
     2761 ctcgcgctgg cgtgggtcgg ggcggcccca gcgaatgctg tgggaaccga cgggtcgatc
     2821 aggacgctgc ccgagacggg cgccaagcgg gacgcctccg gcactccgct cgacggcgta
     2881 gtgggctcgg tcgggtcatc cgtctagcag accgctagat gtggggcacg aactcctgcc
     2941 aggcgactcg tcgcgcccgg cgcgggtctt cctccacgcg atcggttcgg tcgatgatca
     3001 gcgtgcgccg cttcttggcg gtgtagcgcg gccaatcgga tcccacctcc ccggtgcggg
     3061 cgaaactgag ccagtgccgc tgcatgcgca cccccgcgcg tttgaaagcc gcgcgtccac
     3121 cggcggctcc gatgccgcga ccgaagaggc cgtccatccg gtcgaagaga gcgaagagct
     3181 cgagcccgtg cgtggcgtcc agacccgccc agcgcagcag gcggggcgcg atgtcaaacc
     3241 ggtagaagta cacgggggcg aatcgcgcgt gccgctcggc caccttcacc gaggggtacc
     3301 agaacgagta gtcgccagcg aagtcggccg ccggtcgggg cgcgggcaag cccgggtatt
     3361 gggccttgac cgccttgcgc gagcgtttgc gggtcttcgc gaagatcgca cggatgcgcg
     3421 gcggcgtggt cgcgaggatg tccagtcgtc cgcggaacag cgatccctcc cgatcgttgg
     3481 tgccgatgat caggggaacg cgatgggcgc gcccggcttt gaaggcgtcg aggggacgct
     3541 cgggcacgaa ctcgccgtcg atcaccggcg cgagcgcgat cgttcccggc gtctcgtcgg
     3601 gcgcgcgcag ttgcatcttc gtggtcgccg cgaccagctg atcgaccggg gcggtcatca
     3661 gcagctggcc ggcgtcctcc
//
LOCUS       DAOSOU010000211         3666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_25308_length_3666_cov_4.902243, whole genome shotgun sequence.
ACCESSION   DAOSOU010000211 DAOSOU010000000
VERSION     DAOSOU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25308_length_3666_cov_4.902243"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLW15_10070"
     CDS             complement(<1..264)
                     /locus_tag="PLW15_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34778.1"
                     /translation="MASLLSRLAPLVITLRGSKREFSSPERTLARIDRLSRRPRSFEP
                     PRLRDVEVSSRSDASGWPVFTVAPVDPDARSGRRAIYLHGGVYC"
     gene            complement(274..447)
                     /locus_tag="PLW15_10075"
     CDS             complement(274..447)
                     /locus_tag="PLW15_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34779.1"
                     /translation="MNILGFLVSIIIFVGGFYLMGSSFYVPGFEAAVFFLGIITSAIG
                     FAIPVHLLKRIDG"
     gene            602..775
                     /locus_tag="PLW15_10080"
     CDS             602..775
                     /locus_tag="PLW15_10080"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT34780.1"
                     /translation="MNESELPPFGWYPDPAGSGMLRWWNGTAWTTDVQRARPEVQPAF
                     GYSTRDMNRPLAS"
     gene            978..1169
                     /locus_tag="PLW15_10085"
     CDS             978..1169
                     /locus_tag="PLW15_10085"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT34781.1"
                     /translation="MSTITENTADLAPFGWYPDPAGSEMMRWWDGKRWTTHLERPRPE
                     MQPAYGYGSDGSVTQQYEY"
     gene            complement(1185..1826)
                     /locus_tag="PLW15_10090"
     CDS             complement(1185..1826)
                     /locus_tag="PLW15_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT34782.1"
                     /translation="MLRVVVADDSVLLREGLVRVLIEAGVDVVGSYGDADSMLAQLEA
                     LHPDLIVLDVRMPPTYRDEGVRAAIRAREVQPDVGILLLSQYVEVAYAQELLSSGRGG
                     VGYLLKDRVASLAQLQDAVERIAAGGTVLDPEVVAQLLGRRHDPLATLTPREHEVMSL
                     MAEGRTNAAIAQALVIGTGAVEKNVTSIFQKLGLDDSGTDHRRVLAVLAWLQR"
     gene            complement(1837..3162)
                     /locus_tag="PLW15_10095"
     CDS             complement(1837..3162)
                     /locus_tag="PLW15_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT34783.1"
                     /translation="MTTATITETAPRPRGYGALWRALPRELLYLLITFPIAVTGFAVT
                     LGLFWAGVGTIVTFFIGLIFLVGALYVARGFGTATVALVEWTTRRPIPRPAWQDATAR
                     SGFLGWLRAVIGNGHYWLYLLHTALLDFIISTVTWTLTIVWLSIGLGGATSWFWSMFI
                     PVSAQDFYLSRWLFPQGKFDVILVDNLLYLGIGIIFLVTLPLVTRGLTTIHWLVARGV
                     LAAFKSDALLKEVADLTDSRGAAISAEGHSLRRLERDIHDGPQQRLVRLQMDLAAAER
                     QLDTDPEKARGLIAEAMEQSKEALEELRALSRGFAPPILLDRGLIAALESAADRAPIP
                     TRVVSELLADGSLPQEIERNAYFVASEALANAAKHSGATELDVRVGLDDGWLVVAVTD
                     NGVGGAVATPEHGIAGLEERLRGLGGTLEVVSPAGGPTVITARLPVPAT"
     gene            complement(3258..>3666)
                     /locus_tag="PLW15_10100"
     CDS             complement(3258..>3666)
                     /locus_tag="PLW15_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT34784.1"
                     /translation="VAAQHGPRWVPQTGQVTGFELTAAGEPSVWLSGDTVLTDAVREA
                     ARSMRGIDVAVINAGGVRFPSAPLVGKALFTFTPEQFVEVCRLVDPRVIIPVHRSGWS
                     HFQPENDLRRALDAAGLLERTRWLGLGESTEIV"
BASE COUNT          638 a         1259 c         1264 g          505 t
ORIGIN      
        1 gcagtagacg ccaccgtgga ggtagatcgc gcggcggccg gaacgggcat ccgggtcgac
       61 gggtgcgacg gtgaagacgg gccagcccga cgcgtctgac ctcgacgaca cctcgacgtc
      121 gcgcagtcgc ggcggctcga acgaccgcgg gcgacgactc aaccggtcaa tgcgggcgag
      181 agtgcgctcc ggggagctga actcgcgctt ggaccccctg agggtgatga ccaggggcgc
      241 gaggcgactc aggaggctcg ccacggggac gggtcagccg tcgatgcgct tgagcaggtg
      301 cacggggatc gcgaagccga tggcgctcgt gatgatgccc aggaagaaca cggcagcctc
      361 aaagccgggc acgtagaacg aggagcccat gaggtagaag ccgccgacga agatgatgat
      421 cgagaccagg aatccgagga tgttcacgct gtcatcctag cgataccgcc acgcccccca
      481 agtgggggta tcgggtacag ccggtgtccc ctacagtgag gacaccgtcc cgaaactggc
      541 ccctcacggt gggccgagcc gctcccccaa tcgcccgcgg ctggcactgc aggatccttc
      601 gatgaacgag tctgaacttc ccccgttcgg ctggtacccc gacccggccg gctccggcat
      661 gctgcgctgg tggaatggaa cggcctggac caccgacgtg cagcgcgccc gccccgaggt
      721 gcagcccgcg ttcgggtact cgacgcgcga catgaaccgg ccgctcgcct cctgagccgc
      781 cgttcgttcg ggcgggttcc gcccgtgttc gcgattgtcc cgccgtggga gcgctcccac
      841 catccaccga gttgggggct gccgggggac agatagtgtc ctctacagtg gggacaccgc
      901 accacccgac ggtctccagc ggtaggccaa ccgccgctcc cgtacagccc ccgcggcagg
      961 gcactgcagg acccgacatg tcgaccatta ccgagaacac agccgatctc gctccgttcg
     1021 gctggtaccc cgaccccgca ggctccgaga tgatgcgctg gtgggacggc aagcgatgga
     1081 ccacccacct cgagcgtccg cgccccgaga tgcagcccgc ctacggctac ggcagcgacg
     1141 gatccgtcac gcagcagtac gagtactagg acggccggca gcgctcagcg ctgcaaccac
     1201 gccagtaccg cgagcacacg acggtggtcg gtgcccgagt catccagccc cagcttctgg
     1261 aagatggatg tcacattctt ctcgaccgcg ccggtgccga tgacaagcgc ctgagcgatc
     1321 gcggcattcg tgcgcccctc agccatgagg gacatcacct cgtgctcgcg cggtgtgagg
     1381 gtcgccagcg ggtcatgccg gcggccgagc agctgcgcga ccacctccgg gtcgagcacc
     1441 gtgcctccgg cggcgatgcg ctccacggcg tcctgcagct gcgcgaggga tgcgacgcgg
     1501 tccttgagca ggtaccccac tcccccgcgt ccactcgaga gcagctcctg ggcgtaggcg
     1561 acctcgacat actgcgacag caacaggatg ccgacgtcgg gctgcacctc acgcgcacgg
     1621 atcgccgcac gcaccccctc gtcgcgatac gtcgggggca tgcgcacgtc gagcacgatc
     1681 agatccggat gcaacgcctc aagctgcgcc agcatcgagt cggcgtcgcc gtacgacccc
     1741 accacgtcga ccccggcctc gatgagcacc cgaacgagcc cttcgcgtag cagcaccgag
     1801 tcatcggcga ccaccactct gagcatgcgc cccagtttag gtggcgggca ccggcagccg
     1861 tgcggtgatg acggtcgggc cgcccgcggg gctcacgacc tcgagggtgc cgcccaggcc
     1921 acgcagccgc tcctcgaggc ctgcgatgcc gtgctcgggg gtggcgaccg cgccgcccac
     1981 gccgttgtcg gtgacggcga cgaccagcca gccgtcatcg agcccgaccc gcacgtcgag
     2041 ctcggtggca ccggagtgct tcgcggcgtt ggcgagtgcc tcgctcgcaa cgaagtaggc
     2101 gttgcgttcg atctcctggg gcagggaacc gtcggccagc agttcactca ccacccgggt
     2161 cgggatgggc gcgcggtcgg cggccgactc gagtgcggcg atgagaccgc ggtcgagcag
     2221 gatcggcggc gcgaacccgc gtgacagcgc ccgcagttct tcgagcgcct ccttcgactg
     2281 ctccatcgcc tcggcaatga gaccgcgcgc cttctcgggg tcggtgtcga gctggcgctc
     2341 ggcggcagca aggtccatct gcaggcgcac cagtcgctgc tgcggtccgt cgtggatgtc
     2401 gcgctccagc cgacgcagcg agtgaccctc agcggagatg gcggcgccac gcgagtcggt
     2461 gaggtcggcg acctccttga gcagggcatc cgacttgaag gcggccagca ccccgcgggc
     2521 gacgagccag tggatggtgg tgagaccgcg ggtgaccagc ggaagcgtca cgaggaagat
     2581 gatgccgatg cccaggtaga gaaggttgtc gacgagaatc acgtcgaact tgccctgggg
     2641 gaagagccag cgcgagaggt agaagtcctg cgcgctcacc gggatgaaca tactccagaa
     2701 ccacgaggtt gccccgccga ggccgatcga gagccagacg atcgtgagcg tccacgtgac
     2761 cgtcgagatg atgaagtcga gaagtgccgt gtgcagcagg tagagccagt agtgcccgtt
     2821 gccgatgacc gcccggagcc agcccaggaa cccgctgcgc gccgtcgcat cctgccatgc
     2881 cggtcgcggg atcggacgac gggtggtcca ctcgacgagg gccacggtcg cggtgccgaa
     2941 cccacgcgcg acatagagcg cgccgaccag gaagatgagc ccgatgaaga acgtcacgat
     3001 cgtgccgacc ccggcccaga agagtccgag ggtcaccgcg aagccggtga ccgcgatcgg
     3061 gaaggtgatc agcaggtaga gcaactcgcg cgggagggcg cgccagaggg ctccatagcc
     3121 gcgcgggcgg ggtgcagttt cggtgatggt ggctgttgtc atgacggtct cccttgattg
     3181 tctcaagcct cgcgaccacc gccgggatca gccatgcggc atccacacca atcgggggtg
     3241 ggggtaaccc ccgggtgtca cacgatctcg gtcgactcac cgaggccgag ccaccgagtg
     3301 cgctcgagca gcccggcagc atcgagggcc ctgcgaaggt cgttctccgg ctggaagtgg
     3361 ctccagcccg agcggtgcac cggaatgatg acccgcgggt cgacgagacg gcacacctcg
     3421 acgaattgct cgggggtgaa ggtgaacagg gccttgccga cgagcggcgc cgacgggaac
     3481 cgcaccccgc ccgcgttgat gacggcgacg tcgatgccgc gcatgctgcg ggccgcctcc
     3541 cgcacggcgt cggtcagcac ggtgtcgccc gagagccaca cactcggctc gccggctgcg
     3601 gtcaactcga accccgtgac ctgcccggtc tgcggcaccc accgtggtcc gtgctgggcg
     3661 gccacc
//
LOCUS       DAOSOU010000212         3602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_25915_length_3602_cov_4.486045, whole genome shotgun sequence.
ACCESSION   DAOSOU010000212 DAOSOU010000000
VERSION     DAOSOU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25915_length_3602_cov_4.486045"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLW15_10105"
     CDS             complement(<1..250)
                     /locus_tag="PLW15_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34785.1"
                     /translation="MKRAALLLALCLTACAPISDATVADTTIAGRFTPEHPVALTNLT
                     LADGRYRVTYSLTIYAADRGVPRTRLACSVVDVSGRLSD"
     gene            complement(247..1185)
                     /gene="dapD"
                     /locus_tag="PLW15_10110"
     CDS             complement(247..1185)
                     /gene="dapD"
                     /locus_tag="PLW15_10110"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOT34786.1"
                     /translation="MTSRAAWGYGLATVAGDGTVLDTWYPEPHLGVRPSSADPYIAPA
                     EFEKLEGADERRNVTLRFVTVEIDLDEAPASTSDAYLRLHLLSHLLAQPNTLNLDGLF
                     AHLPIVVWTNAGPIHPTDFARMRPQLQRAGIAAHGIDKFPRLLDYVSPDRVRIADASR
                     VRLGAHLAPGTTVMHEGFVNFNAGTLGTSMVEGRVSQGVVVGDGADIGGGASIMGTLS
                     GGGTQRVSIGARALLGANSGVGIAIGDDTVVEAGLYVTAGTKVTVLGTGEPRTVKAVE
                     LSGVAGLLFRRNSLTGAVEVLQRDGTGVTLNTALHA"
     gene            1234..2298
                     /gene="dapE"
                     /locus_tag="PLW15_10115"
     CDS             1234..2298
                     /gene="dapE"
                     /locus_tag="PLW15_10115"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOT34787.1"
                     /translation="MPVLDLAGSVVELTRQLCDIESVSGNERGIADAIEASLAGLAHL
                     ELIRDGDAVVARTSLGRERRVLIAGHIDTVPVNANLPVRVDGDFLVGRGTVDMKAGVA
                     VALKLAAELTEPSVDITWIWYDHEEVASHLNGLGRLAANRPDLLAGDFAVLGEPTSAT
                     VEGGCNGTARVDIRLHGVRAHSARSWVGHNAIHDAAPVLAILAAYVPAEIEVDGLVYR
                     EGLNAVGISGGVAGNVIPDEAVVSVNYRFAPSRSAEDAIAHLHEEFTGYDLEVTDLAA
                     GARPGLDAPLAQDFLAAVGGVASPKYGWTDVARFSALGIPAVNYGPGDPLKAHADDER
                     VDAREIVECERGLRAWLTNG"
     gene            2280..3497
                     /locus_tag="PLW15_10120"
     CDS             2280..3497
                     /locus_tag="PLW15_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34788.1"
                     /translation="MADERLSTTPRVRYRLTPWYVKVLVIFVASRIVTTSLLLAYAAI
                     QPTNAWTGASPNYFDFAAIWDGHWYYIIAAVGYPTELPLTDTGNVGESAWAFMPGYPA
                     VVRLVMFLTWTDFPVAAVIVSVGFALGTALVFYKLMARVLPGGTALFAVVLFCFAPLS
                     PILQVAYAESMHTFLLALALYLLLQRRYWLMLPVIAVMALTRPSGLAFALAMLMHVMY
                     RWWTRAKDPFGVGERVASVAVGLFSAVMGVAWILIAWALTGSPTAYTDTELAWRSAYI
                     GYVDLVPFTPWLQAAGFWAPWFGMPLPLLLVILGLIVVGFFAFLFTPWARRLGPDLRF
                     WLASYALYLLAVFFPQSSTFRLLVPMFPALGAFAVPRAIWYRVGIVLLFIAGQWGWIH
                     IGWWVDGYDWTPP"
BASE COUNT          502 a         1203 c         1272 g          625 t
ORIGIN      
        1 gatcggacag ccgtccgctg acgtcgacga cgctgcaggc gagccgggtg cggggcaccc
       61 cacggtcggc cgcgtagatc gtgagcgagt acgtgacccg gtatcgaccg tcggccagcg
      121 tgaggttggt cagggccacc ggatgctcgg gagtgaagcg cccggcgatc gttgtgtcgg
      181 caaccgtcgc gtcggagatc ggtgcgcagg cggtcaggca gagggcgaga agaagcgccg
      241 cccgtttcac gcgtggagcg cggtgttgag cgtgacgccc gtgccgtcac gctggagcac
      301 ctcgacggcg cccgtcaggg agttgcggcg gaacagcagt ccggcgaccc cgctgagctc
      361 gaccgccttg acggtgcgag gctcaccggt gccgagcacg gtcaccttgg tgccggcagt
      421 gacgtagaga ccggcctcga ccacagtgtc gtcgccgatg gcgattccga ctccggagtt
      481 cgccccgagc agcgcgcgtg cgccgatcga cactcgctgg gtgccgccgc cggagagggt
      541 gcccatgatg gatgcgcccc cgccgatgtc agccccgtcg ccgacgacga cgccctgtga
      601 cacgcgtccc tcgaccatcg acgtgccgag cgtgcccgcg ttgaagttga cgaacccctc
      661 gtgcatcacg gtggtgcccg gagccaggtg ggcaccgagc cgcacccggg acgcgtcggc
      721 gatgcgcacg cggtcggggg agacgtaatc gagcaggcgc gggaacttgt cgatgccgtg
      781 ggcggcgatg ccggcacgct gcaactgcgg gcgcatccgg gcgaagtcgg tcgggtggat
      841 ggggccggca ttggtccaca cgacgatcgg aaggtgcgcg aacagcccgt cgaggttgag
      901 ggtgttgggc tgcgcgagca ggtggctgag caggtgcagc cgaaggtagg cgtcgctggt
      961 ggaggcgggc gcctcatcca ggtcgatctc gactgtgacg aagcgcaggg tgacgtttcg
     1021 gcgctcgtcg gcaccctcga gcttctcgaa ctcggccggc gcgatgtacg gatccgcgct
     1081 cgaggggcgc acgcccagat gcggctcggg gtaccaggtg tcgagcacgg taccgtcgcc
     1141 ggccacggtc gccagaccgt agccccaggc tgcgcgtgag gtcatgtttc ctagagtact
     1201 taaactggct aggtgcccgt caccgacccc accctgcccg tgctcgatct cgcgggctcg
     1261 gtcgtcgagc tcacccgcca gctgtgcgac atcgagtcgg tgtcgggcaa cgagcggggg
     1321 atcgccgatg cgatcgaggc gagtctcgcc ggattggctc atctggagct gatccgtgac
     1381 ggtgacgcgg tggtcgcgcg cacctcgctg gggcgtgaac ggcgggtgct catcgccgga
     1441 cacatcgaca ccgtgcccgt caatgccaac cttccggtgc gggtcgacgg cgacttcctg
     1501 gtgggtcgcg gcaccgtgga catgaaggcg ggcgtggccg tcgccctcaa gctcgccgcc
     1561 gagttgaccg agccgagcgt cgacatcacg tggatctggt acgaccacga ggaggtcgcg
     1621 agccacctca acggactcgg gcgactcgcc gcgaaccgtc ccgacctgct ggcgggcgac
     1681 ttcgcggtgc tcggcgagcc gacgagcgca accgtcgagg gtggttgcaa cggcacggca
     1741 cgtgtcgaca tccgactgca cggcgtgcgt gcgcacagcg cccggtcgtg ggtcggtcac
     1801 aacgccatcc acgatgcggc gcccgtgctc gccatcctcg ccgcctacgt gccagccgag
     1861 atcgaggtcg acgggttggt gtaccgcgag ggactcaatg ccgtgggcat ctcgggcggg
     1921 gtggcgggca acgtcatccc cgacgaggcc gtcgtcagcg tcaactaccg cttcgccccg
     1981 agccggtctg cggaggacgc gatcgcccac ctccacgagg agttcaccgg ctacgacctc
     2041 gaggtcaccg acctcgccgc cggggcgcgg cccgggctgg acgcgcccct ggcccaggac
     2101 ttcctcgccg ccgttggcgg ggtggctagt cccaagtacg gctggaccga tgttgcgcgc
     2161 ttctcggccc tcggcatccc cgccgtcaac tacggccccg gtgacccgct gaaggcccac
     2221 gccgacgacg agcgtgtgga tgctcgggag atcgtcgagt gcgagcgagg gctgcgggcg
     2281 tggctgacga acggctgagc acgaccccga gggtgcgcta tcggctgacg ccgtggtacg
     2341 tgaaggtcct cgtgatcttc gtggcgtcac ggatcgtgac gaccagcctg ctgctcgcct
     2401 atgccgcgat tcagccgacc aacgcctgga ccggcgcgag ccccaattac ttcgacttcg
     2461 cggccatctg ggacggccac tggtactaca tcatcgcggc cgtcggttac ccgaccgagt
     2521 tgcccctcac cgacaccggc aacgtgggcg agagcgcgtg ggccttcatg cccgggtatc
     2581 ccgcggtcgt gcgcctcgtg atgttcctca cctggaccga cttcccggtg gccgccgtca
     2641 tcgtctcggt cggcttcgcg ctcggcaccg cgctggtgtt ctacaagctc atggcccggg
     2701 tgctgccggg cggcaccgcc ctgttcgccg tggtgctgtt ctgcttcgca cccctgtcgc
     2761 cgatcctgca ggtcgcctac gcggagtcga tgcacacctt cctgctcgca ctcgcgctct
     2821 acctgctgct ccagcgccgg tactggctga tgctgcccgt catcgccgtg atggctctga
     2881 cacggcccag cgggctcgcc ttcgccctcg cgatgctcat gcacgtgatg taccggtggt
     2941 ggacccgagc caaggacccg ttcggggtgg gggagcgcgt ggcatccgtg gccgtcggtc
     3001 tgttcagtgc ggtgatgggt gtcgcatgga tcctcatcgc ctgggctctg acgggctcgc
     3061 cgaccgccta caccgacacc gagctggcct ggcgctcggc ctacatcggc tacgtcgacc
     3121 tggtgccgtt cacgccgtgg ctgcaggccg ccggcttctg ggctccatgg ttcgggatgc
     3181 cgctgccgct gctgctggtg atcctcggcc tcatcgtggt cggcttcttc gccttcctgt
     3241 tcacgccgtg ggccaggcgg ctcgggccgg acctgcggtt ctggctggcc agctacgcgc
     3301 tctacctgct cgcggtgttc ttcccgcagt ccagcacttt ccgactgctg gtgccgatgt
     3361 tccccgcgct cggggccttc gctgtgccgc gagcgatctg gtatcgggtg gggatagtgc
     3421 tgctgttcat cgccggacag tggggctgga tccacatcgg ctggtgggtg gacggctatg
     3481 actggacccc gccgtgatct gttaaccttc gcgcggtttt ccggtagccg aggaatgaac
     3541 gataatggag acgctgtgtt tttcgtcaca gcgcgcgaaa ggggtatctg atggcagcca
     3601 tg
//
LOCUS       DAOSOU010000213         3579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_26143_length_3579_cov_5.176788, whole genome shotgun sequence.
ACCESSION   DAOSOU010000213 DAOSOU010000000
VERSION     DAOSOU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26143_length_3579_cov_5.176788"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLW15_10125"
     CDS             <1..263
                     /locus_tag="PLW15_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT34789.1"
                     /translation="TGNNAVVFSHGAAIRVWVAGRAINVPPSFAGEHDIQNTGVVELD
                     GSLESGFTLLSWQGTPVGGSDLSDDTAEDPTGETLDEARAAD"
     gene            complement(264..1544)
                     /locus_tag="PLW15_10130"
     CDS             complement(264..1544)
                     /locus_tag="PLW15_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOT34790.1"
                     /translation="MTLLARDAGLELDHDVTRVVAQLFLPGESIAPMKPQAELIAERV
                     RALAPEQARQIARDLLDAFGPRHPDLEALFGRNADYVLGRIGEHMESGSAHHTVMGGT
                     VTNEYSVEGAALCNPSIAPHPDQTGLLDGQLRVVVSLRQIGEGHISSLGFVTGVIGPG
                     ELFTFDSRHTPLVTPTITSAEGRWSSYRAEFPPQAALSQRVLMPVIDDERNGIEDARL
                     VLFTYDDGSTEYRATYTAYDGRSIIPRLLTSRDLLSFESHPLTGPAATNKGIAPFPRP
                     IKGELYALVRSDGETNGLAHSPDGLAWGRETAIRGPVLPWEIVKSGNCGSPIETDEGW
                     LVITHGVGPMRVYSMGAMLLDLDDPRRVISVLPEPMIRSTGAMQPGYVPNVVYSCGGI
                     LHDGVVWLPHGVGDNRIRVASVGLTELLDAMVAV"
     gene            complement(1541..2569)
                     /locus_tag="PLW15_10135"
     CDS             complement(1541..2569)
                     /locus_tag="PLW15_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34791.1"
                     /translation="MSVTGTTLHRRISSPVYSHLVSLSDDLGLFEHADGDVPRQEHGY
                     CVDDVARALIVAVREPSDQLAALATTSLGFLARAIDDGGRSHNRMSVTGEWTDEPDLG
                     DWWGRSAWALGTAAARNPDLRWRARAHFMRLAATRSPHVRSMAFAALGAAELVLAGDR
                     DRRLVYLLLDALDIVPQQPTNVWAWPEDRLRYGNGSLAEAVIAAGAALEKPAVLEGGL
                     AMLDALLDLETRDDHLSVAGTAGRGPADIAAQFDQQPIEIAAIADAAARAHDITGDPR
                     WLNAVGLAWRWFLGTNDSATPMVDLATGAGYDGLERTGRNDNRGAESTLAALSTWQQA
                     HRLGVLEQ"
     gene            complement(2566..>3579)
                     /locus_tag="PLW15_10140"
     CDS             complement(2566..>3579)
                     /locus_tag="PLW15_10140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT34792.1"
                     /translation="IVRVMDGPVDDSEARFGSRLPIAAHLVAGDPSSMSASIRALNAC
                     DVAIVQHEYGIFGGTDGDEVIGVLEQLTAPAIVVLHTVLEKPTPNQKRVLERVCELAT
                     SVVVMSDRARTTLEATYAVDLRRVQMIPHGVPDPMFSTAEIDGHRIVTWGLIGPGKGI
                     EWGIRALALLGDLDPLPEYVIVGRTHPKVLAHEGEAYRDSLKEIARELGVEAMVTFVD
                     EYLDAAALAEYVTSADVVLLPYDNHDQVTSGVLVEAVAAARPVVATAFPHAVELLTGG
                     AGLVVDHGNPQAIAAALRTVFSGQGRLMSEVATRVSLGTSWTEIAARYDAVAATILAS
                     RAA"
BASE COUNT          564 a         1191 c         1237 g          587 t
ORIGIN      
        1 tcaccggaaa caacgccgtg gtgttcagcc acggtgccgc gatccgcgtg tgggtggcag
       61 ggcgtgccat caacgtgccg ccgagcttcg ccggcgagca tgacatccag aacaccggcg
      121 tggtcgaact cgatggctca ctcgagtcgg gcttcaccct gctgtcgtgg cagggtaccc
      181 ccgtcggtgg ttccgacctg tccgacgaca cggccgagga tccgacaggc gagaccctcg
      241 acgaggcacg cgcggcggac tgactacacc gcgaccatcg cgtcgagcag ctcggtcaga
      301 ccgactgagg cgacccggat gcggttgtcc ccgacaccgt gcggcagcca gacgactccg
      361 tcgtgcagga tgcctccgca cgagtacacg acgttgggca cgtaacccgg ctgcatcgcg
      421 cccgtcgaac ggatcatcgg ttcgggaagt accgagatca ctctcctcgg atcgtccaga
      481 tcgagaagca tcgctcccat ggagtagacc cgcatcggcc cgactccgtg ggtgatcacg
      541 agccagccct cgtctgtctc gatcgggctg ccgcagtttc cggacttcac gatctcccac
      601 ggcagcactg gccctcggat ggcggtctcg cgcccccagg cgaggccatc gggggaatgc
      661 gcgagaccgt tcgtctcgcc atccgagcgc accagtgcgt agagctcgcc cttgatcggg
      721 cgcgggaacg gcgcgatgcc cttgttcgtt gccgcggggc cggtgagcgg gtggctctcg
      781 aacgagagga ggtccctcga ggtcagcagc cgcgggatga tcgatcgccc gtcgtacgcg
      841 gtgtaggttg ctcggtactc ggtgctgccg tcgtcgtagg tgaagagcac caggcgtgcg
      901 tcttcgattc cgttgcgctc atcatcgatc acgggcatga gcacgcgctg gctcagcgcg
      961 gcctgcggcg ggaactcggc acggtaactc gaccaacgac cctccgccga cgtgatcgtc
     1021 ggcgtcacga gcggcgtgtg ccgactgtcg aaggtgaaca actcccccgg cccgatgaca
     1081 ccagtgacga agcccaggct tgagatgtgt ccctcgccga tctggcgcag cgacacgacc
     1141 actcgcagtt ggccatcgag cagaccggtc tggtctgggt gcggtgcgat gctcgggttg
     1201 cacagcgcgg caccctcgac cgagtactcg ttcgtgacgg tgccgcccat gaccgtgtga
     1261 tgggcgctgc ccgactccat gtgctcaccg atgcgaccca gcacgtagtc ggcgttgcgc
     1321 ccgaacaggg cttcgagatc cggatgcctc ggcccgaaag catcgagcag gtcgcgggcg
     1381 atctggcgcg cctgttccgg tgccagcgca cgtacccgct cggcgatgag ttctgcctgg
     1441 ggtttcatcg gagcgatgct ctcgccgggg aggaacagtt gggcgacgac gcgcgtcacg
     1501 tcgtggtcga gctcgaggcc tgcgtcccgt gcgaggaggg tcactgttcc agcaccccca
     1561 ggcggtgcgc ctgctgccag gtgctgaggg cggcgagagt cgactccgct ccccggttgt
     1621 cgttgcggcc ggtgcgttcc aacccgtcgt aaccggcgcc cgtggcgagg tcgaccatgg
     1681 gggtggcgct gtcgttggtc cccaggaacc agcgccaggc cagccctacg gcgttcagcc
     1741 agcgcgggtc gcccgtgatg tcgtgggctc gggccgcggc atccgcgatt gcggcgatct
     1801 cgatgggctg ctggtcgaac tgggctgcga tgtctgccgg gccgcgcccc gctgtgccgg
     1861 ccacagagag gtgatcgtca cgggtctcga gatcgagaag cgcatcgagc atggcgaggc
     1921 cgccctcgag cacggcgggc ttctccagtg cggccccggc ggctatcacc gcctcagcaa
     1981 gcgatccgtt cccgtagcgc agacgatcct cgggccaggc ccacacgttg gtgggctgtt
     2041 gcggcacgat gtcgagtgcg tcgaggagca ggtacacgag acgccggtct cggtcccccg
     2101 cgagtacgag ttccgcggcg cccagagcag cgaacgccat ggatcgcacg tgaggcgatc
     2161 gggtggcggc caagcgcatg aagtgggcgc gagcgcgcca gcgcaggtcg gggttccgcg
     2221 ccgccgcggt gcccagcgcc cacgccgaac gcccccacca gtcgcccagg tcgggttcat
     2281 cggtccactc gcccgtgacc gacatgcggt tgtgtgaacg cccaccgtcg tcgattgcgc
     2341 gggccaggaa tcccagagag gtcgttgcga gtgcggcgag ctggtcgctg ggctcgcgca
     2401 cggcgacgat cagtgcccgc gcgacgtcat cgacgcagta accgtgctcc tgacggggca
     2461 catcaccatc ggcgtgctcg aacagaccga ggtcatcgct caggctcacg agatggctgt
     2521 acaccggcga cgagatccgg cggtgcagcg tcgtgcctgt cacgctcacg cggccctgct
     2581 tgcgaggatc gtagcggcga ccgcgtcgta ccgcgcggcg atctcggtcc agctggtgcc
     2641 gaggctcacc cgcgtcgcca cctccgacat cagacggccc tgcccggaga agacggtgcg
     2701 cagcgcggcc gcgatcgcct gcgggttgcc gtggtcgacg accagaccgg cgccgccggt
     2761 gagcagttcc acggcgtgcg gaaaggcggt ggcgacgacc gggcgggcgg cggcgaccgc
     2821 ctcgaccagc actcccgacg tcacctgatc gtggttgtcg tacggcagga gcacgacgtc
     2881 ggccgacgtc acgtattcgg cgagggccgc ggcatccagg tactcgtcga caaatgtgac
     2941 cattgcctcg acaccgagtt cacgggcgat ttctttcagc gagtcccggt aggcctcgcc
     3001 ttcgtgggcg agcaccttcg gatgggttct gccgacgatc acgtattcgg gcagcgggtc
     3061 gaggtcaccc aatagcgcga gggcgcggat tccccactcg atacccttgc ccggtccgat
     3121 gagtccccag gtgacgatgc ggtggccgtc gatctccgct gtgctgaaca tcgggtcggg
     3181 aacaccgtgc gggatcatct gcacccgacg caggtcgacg gcgtaggtgg cttcgagggt
     3241 ggtgcgagct ctgtccgaca tgaccaccac ggaggtggcg agctcgcaga cgcgctcgag
     3301 aacgcgcttc tggttggggg tgggcttttc cagcaccgtg tgaagtacca cgatcgcagg
     3361 ggctgtgagt tgttcgagca cgccgatgac ctcgtcaccg tcggtgccac cgaagattcc
     3421 gtactcgtgc tggactatcg cgacatcgca ggcattgagg gcccggatgc tcgcactcat
     3481 gctcgaggga tcccccgcca cgaggtgcgc ggcgatcggc agccgggagc cgaagcgcgc
     3541 ctcggagtca tcgacgggtc catccatcac acgcacgat
//
LOCUS       DAOSOU010000214         3513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_26794_length_3513_cov_4.695200, whole genome shotgun sequence.
ACCESSION   DAOSOU010000214 DAOSOU010000000
VERSION     DAOSOU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26794_length_3513_cov_4.695200"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLW15_10145"
     CDS             <1..222
                     /locus_tag="PLW15_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HOT34793.1"
                     /translation="IHFTWRFEPDGVLAVLPVVEQALAPFGARPHWGKLFHAVDRSLY
                     PRLGDFVELAERLDPEHKFGNEWLDRHVF"
     gene            complement(230..1195)
                     /locus_tag="PLW15_10150"
     CDS             complement(230..1195)
                     /locus_tag="PLW15_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34794.1"
                     /translation="MKILSAALVTAGAFALALAGAAPAMAAGRDIDPGDSMYTISCDA
                     LVDIYNDWQLMSVAPTTAVSTPIGDGTGNQDLDSCADQPAYDASTGKSYYVQITFDGE
                     VHWALASVNVSTGASTTVAEFLWENGEFPTLPRIDAIAIAPDGTAYAIGDGTLWTLDL
                     TNATVEPIGETLNAYAFASDPRTGEFYAIEYGGDVYIFDDIANSGAVTFVGSITHPDL
                     DLDGDVTGVRALQIDGGGTFWIVVDTREGFSDLWSFTPATLTSPVLSGLLTDDPFFSQ
                     ALLIIPGAALAATGADLSLVPYLAGGAALAVIAGGAVLLIRRRRA"
     gene            complement(1335..2288)
                     /locus_tag="PLW15_10155"
     CDS             complement(1335..2288)
                     /locus_tag="PLW15_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOT34795.1"
                     /translation="MKPLSAVLAATGAAALILIGASPAAAAGSSIDPGDSLYAIDCDD
                     TVYNDWQLMKVEPSTAASTPIGDGSGSTSEDFFACAGQPAYDASTGKSYYIQWQYDTE
                     PQQYFLASIDLATGNSTTIGEFFYIDGEFPTYPYVESMAIGLDGSAYAFSQGFLWSVD
                     LSSGELTPIDESLSNTYAFAVDPTTGIFYAIDSSNNRYSVSSDGSYIYLDTVTLPAET
                     TIYSLQIDGGGRFWIEVDDSNLDSDAGLWSFAPADAATPTYSGEFIDDPYYTEALLIV
                     PGAALAATGADFGGVAPLAAGGAALALAGVALLALRRRRAA"
     gene            complement(2363..>3513)
                     /locus_tag="PLW15_10160"
     CDS             complement(2363..>3513)
                     /locus_tag="PLW15_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOT34796.1"
                     /translation="DLIFDSHETWNNKLALARRALAIYRTLRTAGVVTQTAEGTIALT
                     VDLQPNFALNQPLSPFALAVFELLDPESPTYALDMISVLEATLDDPRPILSQQQFQAR
                     GEAVAAMKAEGIEYDERMELLEQITWPKPLEELLDAAFQTYSASQPWIGDFALSPKTV
                     VRDMYERAMTFADFVGYYKLARSEGLVLRYLSDAYRAARQTIPDDAKTDDLRDLIEWL
                     GEVVRQVDSSLLDEWEAMVNPTSVIEPGSVIEPVEIRPPSVLSNPRAFRVLVRNELFR
                     RVQLADLEDWQALGELDAASGFDADRWADSMDAYFDEHGSLGTGPDARGPAMIMITEH
                     PDHWAVRQIFDDPAGDHDWGISATVDLAESAEAGIAVVRVTAVGMTDA"
BASE COUNT          624 a         1079 c         1232 g          578 t
ORIGIN      
        1 atccacttca cctggcgctt cgagcccgac ggtgtgctgg ccgtgctgcc ggtcgtcgag
       61 caagccctcg cgccgttcgg cgcgcgaccg cactggggaa agctgttcca cgcggtcgac
      121 cggagcctgt acccgcgact gggcgacttc gtggagctgg ccgagcgact cgaccccgag
      181 cacaagttcg gcaacgagtg gctcgaccgc cacgtgttct aggtggtgct taggcgcggc
      241 ggcggcgaat cagcagcacc gcgccgcctg cgatcaccgc aagcgcagca ccgccggcga
      301 ggtacggcac gaggctcaga tcagcgcccg ttgcggccag ggcagcgccg gggatgatga
      361 gcagggcctg actgaagaac gggtcatccg tcaggaggcc ggagagcaca ggtgaggtca
      421 gagttgccgg ggtgaagctc cacaggtcgc tgaatccctc acgggtgtcg acgacgatcc
      481 agaacgtgcc gccgccatcg atctgtagcg cgcgaacacc ggtgacgtca ccgtcaaggt
      541 cgaggtcggg atgagtgatc gagcccacga acgtgaccgc accgctgttc gcgatgtcgt
      601 cgaagatgta gacgtctccc ccgtactcga tcgcatagaa ctcgcccgtg cgagggtcgg
      661 acgcgaacgc gtacgcgttg agggtctcgc cgatcggttc cactgtggca ttggtgaggt
      721 cgagggtcca cagagtgccg tcgccgatgg cgtaggcagt gccatccggg gcgatggcga
      781 tcgcatcgat cctcgggagg gtggggaact cgccgttctc ccagaggaat tcggcaacgg
      841 tcgtgctcgc accggtcgag acgttgaccg acgcgagcgc ccagtgcacc tcgccgtcga
      901 acgtgatctg cacgtagtag gacttgcccg tggacgcgtc gtaggcgggc tggtcggcgc
      961 acgagtcgag gtcctggttt cctgtgccat cgccaatcgg cgtcgaaacc gcggtagtcg
     1021 gcgcgaccga catcaactgc caatcgttgt agatgtcgac gagagcgtcg cagctgatcg
     1081 tgtacatcga gtcgcccgga tcgatgtcac gccccgccgc catggcgggg gcggcaccgg
     1141 cgagagcgag cgcgaacgcg ccagcggtga cgagggcagc ggacagaatc ttcatggggg
     1201 ggcgtctccg attcgggcgg tgtggcttgc gggccagcgt agtccttacc gcgagatatc
     1261 gaaatgcctt cagcacaaga cgccgaaagc ccgcctcgcg ttcaacgcga ggcgggcttt
     1321 cgtgaagcgg tgcgtcaggc ggcgcggcga cgacgcagcg ccagcagcgc gacgcccgcg
     1381 agggcgagcg cagcgccacc ggcggccagc ggggcaaccc cgccgaagtc ggcaccggta
     1441 gcggccagag ccgcgccggg aacgatcaga agcgcctcgg tgtagtacgg gtcgtcgatg
     1501 aattcgcccg agtaggtcgg ggtcgcggcg tccgctggag cgaagctcca gagcccggcg
     1561 tcggagtcga ggttggagtc gtcgacctcg atccagaacc ggccaccacc gtcgatctgc
     1621 agcgagtaga ttgtcgtctc cgcaggaagc gtcacagtgt cgagatagat gtagctgccg
     1681 tcgctgctaa cgctgtagcg attgttggag gagtcgatcg catagaagat ccctgtggtg
     1741 gggtccactg cgaaggcata ggtattggag aggctctcat cgatcggcgt caactcacca
     1801 ctgctcaggt cgacgctcca gaggaagccc tgcgagaacg cgtaggccga gccgtcgagc
     1861 ccgatggcca tcgactccac gtacgggtag gtcgggaatt caccatcgat gtagaagaac
     1921 tcgccgatgg tcgtgctgtt gccggtcgcg aggtcaatcg acgccaggaa gtactgctgg
     1981 ggctcggtgt cgtactgcca ttgaatgtag taggacttgc cggtcgaggc atcgtaggca
     2041 ggctgacccg cgcaggcgaa gaagtcctcc gacgttgaac ccgaaccgtc tccgatgggg
     2101 gtcgaggcgg cggtagaagg ctccaccttc atgagctgcc agtcgttgta gacagtgtcg
     2161 tcacagtcga tcgcgtacag cgagtcaccg gggtcgatcg agctgccagc ggctgcggcc
     2221 ggggacgcgc cgatcaggat gagggctgcg gcaccagtgg cggcaagcac ggcggacaga
     2281 ggcttcatgg gagtctcctg aaagttcggg taagcaaggg ttggctaatg ctatacccgc
     2341 gggcacgtat cgtgaagcag actcacgcgt ccgtcattcc gaccgccgtc acccgcacca
     2401 cggcgatacc ggcctcggcg gactcggcga ggtccacggt tgcgctgatg ccccagtcgt
     2461 gatcgcccgc cgggtcgtcg aagatctgcc gcaccgccca gtgatcggga tgctcggtga
     2521 tcatgatcat cgccgggccc cgggcatccg gacccgtacc gagcgagccg tgctcgtcga
     2581 agtaggcgtc catggaatcc gcccagcggt cggcgtcgaa cccggatgcc gcatcgagtt
     2641 cgccgagcgc ctgccagtcc tcgaggtcgg ccagttgcac ccgccggaac aactcgttgc
     2701 gcaccagcac gcggaaggcg cgcgggttgg agaggaccga cggcggacgg atctcgacag
     2761 gctcgatcac cgacccaggc tcgatcaccg aggtcggatt gaccatcgct tcccactcgt
     2821 cgagcaggct cgagtcgacc tggcgcacga cctcgcccag ccactcgatg aggtcgcgca
     2881 gatcgtcggt cttggcgtca tccgggatcg tctgacgggc ggcgcggtag gcgtcggaca
     2941 ggtaccgcag caccaggcct tcgctgcggg ccagcttgta gtagcccacg aagtcggcga
     3001 aggtcatcgc gcgctcgtac atgtcgcgca ccacggtctt gggactcagc gcgaagtcgc
     3061 cgatccaggg ctggctggcg ctgtaggtct ggaacgcggc atccagaagc tcttcgagag
     3121 gcttcggcca ggtgatctgc tcgagcagtt ccatgcgttc gtcgtactcg atgccctccg
     3181 ccttcatggc ggcgacggcc tcgccgcgag cctggaactg ctgctgggag agtatcggcc
     3241 gagggtcgtc gagggtcgcc tcgagtaccg agatcatgtc gagcgcatag gtcggcgact
     3301 ccgggtcgag aagctcgaac accgccaggg cgaaaggcga cagtggctgg ttgagcgcga
     3361 agttgggctg gaggtcgacg gtgagtgcga tcgtgccctc ggcggtctgc gtcacaaccc
     3421 ccgcagtgcg cagggttcga tagatcgcga gcgcgcgacg cgcgagggcg agcttgttgt
     3481 tccacgtctc gtggctgtcg aagatgaggt cgc
//
LOCUS       DAOSOU010000215         3509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_26834_length_3509_cov_4.521714, whole genome shotgun sequence.
ACCESSION   DAOSOU010000215 DAOSOU010000000
VERSION     DAOSOU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26834_length_3509_cov_4.521714"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLW15_10165"
     CDS             complement(<1..583)
                     /locus_tag="PLW15_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT34797.1"
                     /translation="MTNTDANTNPDVIIIGGGAAGLSAALVLSRARWATLVIDAGEPR
                     NAPATEMHGFLSRDGMPPADLLATGRTEVLGYGGAIQAARVSSVERGADDRFAVTLEG
                     GNIVSARALLVASGLTDELPHIPGLRDRWGGSVHHCPYCHGYEVRGQSIVVIGGANPA
                     VSVRQVGLLRRHSDRVQFVSGGIPLASIDRDRLE"
     gene            661..1248
                     /locus_tag="PLW15_10170"
     CDS             661..1248
                     /locus_tag="PLW15_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT34798.1"
                     /translation="MSTSQRIDTELGQIAPRLRQARQKKDLTLEELSKSTGISKSTLS
                     RLESGQRKPSLELLLPIVAALAVSLDDIVTSPRIEDPRVQQKSTRADGRILTPLSQQH
                     GEPQAFKITIPATDREPALRTHGGYEWIYVLSGRLRLVLGEHDIVMGAGEAAEFDTKN
                     PHWFGASGAGPVEILSMFGKQGERIHLRARSKGTA"
     gene            1245..2321
                     /locus_tag="PLW15_10175"
     CDS             1245..2321
                     /locus_tag="PLW15_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOT34799.1"
                     /translation="MTHRLTVDQARRIAVGAQLLDANRPGDVVGVAEQLGAIKIDPTA
                     TIAPAENTILWSRIGATYEPSQLTKVVEQDHLLFEFDGAFRPISLLPLMLPAMRRWPQ
                     RPKTREWLEANDRFRTDVLARLRADGPLLASDIPDTAQVTRPPDGWYGSNQVVHMLDF
                     LQRQGEVAVVRREGRNRVWDLAERVYPTGMPQYELEEAESLLGDRRLQAAGIAKQKSP
                     WTPVGEAGEPAVIEGSTRKYRVDPQALAALEQDDGGRVAFLNPYDPMLFDRPRLQELF
                     EFEYVLEQFKPKPQRKYGFFAHPILMGDRFVGMLDAEVDREAEMLVVNAVHEFLPFDP
                     EEDEMVRAEIDALAEWLGMGVRAW"
     gene            complement(2328..2906)
                     /locus_tag="PLW15_10180"
     CDS             complement(2328..2906)
                     /locus_tag="PLW15_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOT34800.1"
                     /translation="MSSPVLHVLAGPNGAGKSTFVRDVLLPVTHLPFINADEIASLRW
                     PGEESEHAYEASAAAAAERDKALGERSSFITETVFSHPSKVDLVRQALSEGYLVTLHV
                     ILVPEDVSVLRVAYRVDQGGHAVPEEKVRQRYRRLWTWVAEARDLADLTRFYDNSRSE
                     PAFRAVANYSRGQLVGEATWPAWTPRALMGDV"
     gene            complement(2903..3265)
                     /locus_tag="PLW15_10185"
     CDS             complement(2903..3265)
                     /locus_tag="PLW15_10185"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34801.1"
                     /translation="MKTMPTRIDGDLFEAAKVAGEAQSRSAAQQLDHWVRIGRELDAS
                     PAVTRRQIDDVLAGRTQYDDLTDRAQAVVRATWDEQLLARIEGLSYVERLEAAGQPWA
                     ESDAEGNVVMRNAGSTPA"
     gene            complement(3295..>3509)
                     /locus_tag="PLW15_10190"
     CDS             complement(3295..>3509)
                     /locus_tag="PLW15_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34802.1"
                     /translation="GVDPVQALVATAVINGIVAVPLIFLILKVSSRADVMGAHRSGPW
                     SRLGLWVTFVLMGAAAVALLASFVIG"
BASE COUNT          579 a         1201 c         1195 g          534 t
ORIGIN      
        1 cctcgaggcg gtcgcgatcg atcgaggcca gcgggatgcc gccgctcacg aattgcaccc
       61 ggtcgctgtg gcggcgcagc agcccgacct gccgcaccga caccgccggg ttcgcgccgc
      121 cgatcaccac gatcgactgg ccgcgcacct cgtagccgtg gcagtagggg cagtggtgca
      181 ccgagccgcc ccaacggtcg cggagaccgg ggatgtgcgg cagctcgtcg gtgaggccgg
      241 aggcgaccag aagcgcacgg gcgctcacga tgttgccgcc ttccagagtg acggcgaagc
      301 ggtcgtccgc tccgcgctcg acggatgaga cccgcgcggc ttggatcgcg ccaccatagc
      361 cgagcacctc ggtgcggccg gtcgccagaa ggtcggcggg gggcatcccg tcgcgggaga
      421 ggaagccgtg catctcggtc gcgggggcat tgcggggttc accggcgtcg atcaccagcg
      481 tggcccaccg cgctcgggag agcacgaggg ccgcgctgag gccggcggcc ccgccgccga
      541 tgatgatcac gtcggggttg gtgttggcgt cggtgttggt catggcccca tagttccacg
      601 gggcgtttcg gaattgggag aactgttgcc gaaactggaa caccgtgctt gcatgggcgg
      661 atgagcacct cccagcgcat cgacaccgag ctcggccaga tcgccccacg cctgcgtcag
      721 gcccggcaga agaaggactt gacgctggag gaactctcga agtcgacggg gatttccaag
      781 agcacgctgt cgcggctgga gtccggtcag cgcaagccga gcctcgaact cttgctaccg
      841 atcgtcgcgg ccctggccgt gtcgctcgac gacatcgtca cctcgccgcg catcgaggat
      901 ccgcgggtgc agcagaagtc gacgcgggcc gacggccgca tcctcacacc cctgtcgcag
      961 cagcacggcg aaccgcaggc gttcaagatc accatccccg cgaccgaccg cgagccggcg
     1021 ctgcgcactc acggcgggta cgagtggatc tacgtgctgt ccggtcggct gcgactcgtg
     1081 ctcggcgagc acgacatcgt gatgggggcg ggggaggccg ccgagttcga cacgaagaac
     1141 ccgcactggt tcggtgcgtc gggcgcgggc ccggtggaga tcttgagcat gttcggcaag
     1201 cagggggagc gcatccatct gcgggctcgg tcgaagggga ccgcgtgacc caccggctca
     1261 ctgtcgacca ggcccgtcgc atcgccgtcg gagcgcagct gctcgacgcg aatcgtcccg
     1321 gcgatgtggt cggggtcgcc gaacagctcg gcgcgatcaa gatcgatccg accgccacga
     1381 tcgccccggc cgagaacacc atcctctggt cgcgcatcgg cgccacctac gagccctcgc
     1441 agctgaccaa ggtggtcgag caggaccacc tgctgttcga gttcgacggt gccttccggc
     1501 cgatcagcct gctgccgctc atgcttccgg ccatgcggcg ctggccgcag cgaccgaaga
     1561 cccgcgagtg gctggaggcc aacgaccggt tccgcacgga tgtgctggcc cggcttcgcg
     1621 ccgacggccc gcttctggcc agcgacatcc ccgacaccgc gcaagtcacc cgtccaccgg
     1681 acggctggta cggatccaac caggtcgtgc acatgctcga cttcctgcag cggcaggggg
     1741 aggtggccgt cgtgcgccgc gaggggcgca accgggtgtg ggatctcgcc gagcgcgtct
     1801 acccgactgg catgccccag tacgagctcg aggaggccga gagtctgctg ggcgaccgcc
     1861 gcctgcaggc cgccggaatc gccaagcaga agtcgccctg gacgccggtc ggcgaggcgg
     1921 gcgagccagc cgtgatcgag ggcagcaccc gcaagtaccg ggtcgacccg caggcgctcg
     1981 ccgccctcga gcaggacgac gggggccggg tcgccttcct caacccctac gacccgatgc
     2041 tgttcgaccg cccgcggctg caggaactct tcgagttcga gtacgtgctc gagcagttca
     2101 agccgaagcc gcagcgcaag tacggcttct tcgcgcatcc gattctcatg ggtgatcgat
     2161 tcgtcgggat gctcgacgcc gaagtcgacc gcgaggccga gatgctcgtg gtgaacgcgg
     2221 tgcacgagtt cctgccgttc gaccccgagg aggacgagat ggtgcgcgcc gagatcgacg
     2281 cgctcgccga gtggctgggc atgggcgttc gggcgtggtg acgcccgtca gacgtcaccc
     2341 atgagcgccc gcggcgtcca cgcaggccag gttgcttcac cgaccagctg gccgcgggag
     2401 tagttggcga ctgcacggaa ggcgggttcg ctgcgcgagt tgtcgtagaa gcgcgtgagg
     2461 tcagcgagat cacgcgcttc ggccacccag gtccatagtc gccgataacg ctgccgcacc
     2521 ttctcctcgg gcacggcgtg cccgccctga tcgacccggt aggcgacgcg cagcacagac
     2581 acgtcctccg ggacgaggat cacgtgcaag gtgaccaagt agccctccga cagcgcctgc
     2641 cgtacaaggt caaccttgct cggatggctg aagacggtct cggtgatgaa ggaactccgt
     2701 tcgccaagcg ctttgtcccg ctccgcagcg gcggccgcgg atgcctcgta ggcatgctcg
     2761 gactcctccc ccggccatcg cagcgaggcg atctcatcgg cgttgatgaa tggcaggtgc
     2821 gtgacgggta gaagaacatc ccgaacaaat gtggacttgc ctgccccatt tggccccgcg
     2881 agcacgtgca ggacaggtga actcacgcgg gcgttgagcc cgcgttacgc atgaccacgt
     2941 tgccttccgc atcggactcc gcccagggct ggccagccgc ctctagccgc tccacgtagc
     3001 tgagcccttc gatccgtgcg agcagctgtt catcccaggt tgcccgcacc acagcttgtg
     3061 ctctgtcggt gaggtcgtcg tactgtgtgc gcccggcgag cacgtcatcg atctgccggc
     3121 gggttacggc cggcgaggca tccagctccc gaccgattct cacccagtga tccaactgct
     3181 gcgcagcgct gcggctctga gcttcgcccg caaccttcgc ggcctcgaag aggtctccgt
     3241 caatgcgcgt cggcatcgtc ttcatgtgcc aagtgtagca cttcgctaca cgggtcagcc
     3301 gatcacgaac gacgcgagca gcgccactgc cgcggcaccc atcagcacga atgtcaccca
     3361 cagcccgagg cgtgaccacg gcccgctgcg gtgcgccccc atcacgtcgg cgcgcgacga
     3421 gaccttcagg atgaggaaga tcaggggcac ggcgacgatg ccgttgatga cggctgtcgc
     3481 cacgagcgcc tgcaccgggt cgacgccga
//
LOCUS       DAOSOU010000216         3484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27077_length_3484_cov_5.639253, whole genome shotgun sequence.
ACCESSION   DAOSOU010000216 DAOSOU010000000
VERSION     DAOSOU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27077_length_3484_cov_5.639253"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..563
                     /locus_tag="PLW15_10195"
     CDS             93..563
                     /locus_tag="PLW15_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOT34803.1"
                     /translation="MFRAVRAATAAFMRSPARLVIGPLITPIDRALFHVSGGRLKLSA
                     PMIPSLMLFTTGAKSGLRRESPLMCFPRDDGTWFIVGSNFGLEKHPAWSSNLIANPGA
                     EIHYRRQLVPVRATLLQEAEAEAMWPELEEQWPHYRDYEKTARRDIRIFHLVRR"
     gene            563..1804
                     /gene="hemW"
                     /locus_tag="PLW15_10200"
     CDS             563..1804
                     /gene="hemW"
                     /locus_tag="PLW15_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT34804.1"
                     /translation="MAPSALPIADPAPLDGALPPSVVDDAATRNFGVYLHVPFCRVRC
                     GYCDFNTYTSDELRGAKRTDYAGQAVTEVALAARVLHDAGLPPRPVGTVFFGGGTPTL
                     LPATDLAAMLHSVVDTWGLVDGAEVTTEANPDSVDLDYLLALKRAGFTRVSFGMQSAV
                     PHVLATLERTHDPSRIPLVVEWAREAGLQVSLDLIYGTPGESLADWGESLDQALAQKP
                     DHLSAYALIVEDGTKLAAQIRRGEVAEPDDDLEADMYELADRRLSVSGYEWYEVSNWA
                     LEGGTDGLDRRSRHNLAYWQGHDWWGVGPGAHSHVGGVRWWNVKHPAAYADRIAAGLS
                     PAAGRETLDADTRRVEAVLLRTRLREGMPVAELEASGRHAVAGLIADELVDARAAIAG
                     RLELTLRGRLLADAVVRRLVE"
     gene            complement(1809..2186)
                     /locus_tag="PLW15_10205"
     CDS             complement(1809..2186)
                     /locus_tag="PLW15_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOT34805.1"
                     /translation="MSTTPPPPANPYATPAQPLSPSDEKLWATLIHIGGILFGFLPSL
                     IGYLVLKDRGPFIRAHSATALNFQLTLIIAYAVGTVTSLLLIGLLVLLAAWVASIVLG
                     IVAAMAANRGEAYTYPLSIKFVS"
     gene            complement(2246..2629)
                     /locus_tag="PLW15_10210"
     CDS             complement(2246..2629)
                     /locus_tag="PLW15_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOT34806.1"
                     /translation="MTDATPAPAAPLTPAEDKQWGSFAHLGGIIGFLPSLIIWLIFKD
                     RGTITNVEAKEALNFQITAAIAQVAVFIINAILTAVTFGIWGFIGWLLPLAVWVMSLI
                     WSIMGFQKVNAGGSYRYPVSIRLIK"
     gene            complement(2663..3064)
                     /locus_tag="PLW15_10215"
     CDS             complement(2663..3064)
                     /locus_tag="PLW15_10215"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOT34807.1"
                     /translation="MTDAPPPTVAAAPLTAAEDKLWASLAHFGGIVGILPSLIVFLVF
                     RERGERTKQESREALNWQITFTIGWIALSIIGAIISFVAYSARVYVVGDLVGYLPLIW
                     WIVNAVLSVLGGVKVQGGGSYRYPFALRLVK"
     gene            3210..>3484
                     /locus_tag="PLW15_10220"
     CDS             3210..>3484
                     /locus_tag="PLW15_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT34808.1"
                     /translation="MVSERGLEVLRVIVQDYVASREPVGSKSIVERHSFGVSAATIRN
                     DMALLEDEELIAAPHTSSGRVPTDKGYRVFVDQLADLRPLSPAQRQA"
BASE COUNT          598 a         1108 c         1214 g          564 t
ORIGIN      
        1 agcgcgactg tttccgagaa tcagggttcc gtctatgtcc cacaggatgt acgtgctcac
       61 cgcaccattc tcgcgggtga aagactggtc ccatgttccg agccgtgcgc gcagccacag
      121 cggccttcat gcgcagtccc gcgcgcctcg tcatcggccc gctcatcacc ccgatcgacc
      181 gggcgctgtt ccatgtctcg ggcggccggc tcaagctgag tgcgcccatg attccgtcgc
      241 tcatgctgtt cacgacgggc gccaagtcgg gcctgcgccg cgagtcgccc ctgatgtgct
      301 tcccacgcga cgacggaacc tggttcatcg tcggcagcaa cttcggtctg gagaagcacc
      361 cggcctggag ctccaacctc atcgccaacc ccggcgccga gatccactac cggcggcagc
      421 tggtgcccgt gcgcgccacg ctcctgcagg aggcggaggc cgaagcgatg tggcccgagc
      481 tggaggagca gtggccgcac taccgcgact atgagaagac agcgcggcgt gacatccgga
      541 tcttccacct ggtgaggcgc tgatggcgcc cagcgcactg ccgatcgcgg acccagcacc
      601 tctcgacgga gcactgccgc catcggtcgt ggatgacgcg gccacccgca acttcggggt
      661 ctacctccac gttccgttct gtcgcgttcg ctgcggatac tgcgacttca acacgtacac
      721 gagtgacgag ttgcgcggag ccaagcgcac cgactacgcg ggccaggccg tcactgaggt
      781 ggcgttggcg gctcgcgtgc tgcacgatgc cggcctgcca ccccggccgg tgggcacggt
      841 cttcttcggc gggggcacac cgaccctcct gccggccacc gacctggcgg cgatgctgca
      901 ctcggtggtc gacacgtggg ggctggtcga cggagccgag gtgacgaccg aggcgaaccc
      961 cgactcggtg gatctcgact acctgctggc gctgaagcgc gcgggcttca cacgcgtatc
     1021 cttcggcatg cagtcggccg tgccgcacgt gctcgcgacc ctcgagcgca cgcacgaccc
     1081 ctcgcgcatc cccctggtgg tcgagtgggc gcgcgaggct gggcttcagg tgagcctcga
     1141 cctgatctac ggaacgcccg gggagagcct cgccgattgg ggcgagtccc tcgatcaggc
     1201 tctggcacag aagcccgacc acctctccgc ctacgccctc atcgtcgagg acggcacgaa
     1261 actggctgcc cagatccgtc ggggcgaggt cgccgagccc gacgatgatc tcgaggcgga
     1321 catgtacgag ctcgccgatc gccgcctcag tgtgtcaggg tacgagtggt acgaggtcag
     1381 caactgggcg ctggagggcg gcaccgacgg gctcgatcgc cgctcgcgcc acaacctcgc
     1441 ctactggcag ggccacgact ggtggggtgt cgggcccggc gcgcacagcc acgtcggcgg
     1501 cgtgcgctgg tggaacgtca agcatccggc cgcgtacgcc gaccggatcg cggccgggct
     1561 gtccccggcc gccggacggg agaccctcga cgccgatacg cgacgagttg aggcggtgct
     1621 gttgcgcacg cgtctgcggg aggggatgcc cgtggctgag ctcgaggcat ccggtcgcca
     1681 tgccgtcgcc ggcctcattg ccgacgagct ggtggatgct cgcgcggcaa tcgcgggacg
     1741 actggaactc accctgcgcg ggcgtttgct cgccgacgcc gtcgtgagac ggctggtcga
     1801 gtgagctcct agctcacgaa cttgatcgac agcgggtagg tgtacgcctc gccgcggttg
     1861 gccgccatcg ccgcaacgat gccgagcacg atgctcgcga cccaggcggc gagtagcacc
     1921 agcagtccga tgagcagcag cgacgtcacg gtaccgaccg cgtacgcgat gatcagcgtc
     1981 agctggaagt tgagggcggt ggcgctgtgg gcgcggatga acgggccgcg atccttcagc
     2041 acgaggtagc cgatgagcga gggcaggaag ccgaacagga ttccgccgat gtggatgagc
     2101 gtggcccaga gcttctcgtc gctggggctc aacggctggg cgggagtcgc gtaggggttg
     2161 gcgggcgggg gcggggtcgt ggacatggcc ccattaaagc agcgagcccg gaggcagtgc
     2221 ccccgggctc gccgtcagac gagtgctact tgatgaggcg gatcgacacc gggtagcggt
     2281 aggagccacc ggcgttgacc ttctggaagc ccatgatcga ccagatcagc gacatgaccc
     2341 agacggcgag cggcagaagc cagccgatga agccccagat gccgaaggtg acggcggtga
     2401 ggatcgcgtt gatgatgaac accgcgacct gggcgatggc ggcggtgatc tggaagttca
     2461 gcgcctcctt ggcctcaacg ttggtgatcg tgccgcggtc cttgaagatg agccagatga
     2521 tgagcgacgg caggaagccg atgatgccac cgaggtgggc gaaggatccc cactgcttgt
     2581 cctcggcggg agtcagaggg gcggccgggg cgggagttgc gtctgtcatg gtgacctttc
     2641 gatgggttgg gtttacgtgg aactacttga cgaggcgaag tgcgaacgga tagcggtacg
     2701 aaccgccacc ctgaactttc acgccgccga ggacagagag gactgcgttg acgatccacc
     2761 agataagcgg aaggtaaccg acgagatcgc ccacgacgta cactcgcgcg gagtatgcga
     2821 cgaagctgat gatggctccg atgatactga gggcgatcca gccgatggtg aaggtgatct
     2881 gccagttgag cgcttcgcgg gactcctgct tggtgcgctc gccgcgctcc cggaatacca
     2941 ggaacacgat cagtgaggga agaatcccga caatcccgcc gaagtgagcc agcgatgccc
     3001 agagcttgtc ctccgctgcc gtcagcggcg cggccgcaac ggtagggggt ggagcgtcgg
     3061 tcatcgaacg ggtgcctttc gggggtgttg atggtgtcag cacaaggtat cgctatggaa
     3121 cacggcgtgc aacggtttgg caacggcgag tcacggtaca attggcagtc agcgcaacag
     3181 agtgccaaca gcgagaggcg gtggtcccag tggtctccga gcggggtctt gaggtgcttc
     3241 gcgtcatcgt ccaggactac gtcgcctccc gcgagccggt cggctcgaag tcgatcgtcg
     3301 agcggcactc cttcggtgtg tcggccgcga cgattcgcaa tgacatggct ctgctcgaag
     3361 acgaggagct cattgctgct ccccacacgt cgtccggtcg ggttccgacc gacaaggggt
     3421 accgcgtctt cgtcgaccag ctcgccgacc tgaggccgct gagtccggcc cagcgccagg
     3481 ccat
//
LOCUS       DAOSOU010000217         3481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27112_length_3481_cov_7.298599, whole genome shotgun sequence.
ACCESSION   DAOSOU010000217 DAOSOU010000000
VERSION     DAOSOU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27112_length_3481_cov_7.298599"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLW15_10225"
     CDS             complement(<1..227)
                     /locus_tag="PLW15_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34809.1"
                     /translation="MAREGLPRPGQGAGAQGAGAPGQGASTPVAGQPAPDAAPAPTAD
                     TAPTPPPAAPRPQRTGLGANAKPAASTPAAAT"
     gene            complement(231..1211)
                     /locus_tag="PLW15_10230"
     CDS             complement(231..1211)
                     /locus_tag="PLW15_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT34810.1"
                     /translation="MTDSSAPILVLVEVSPAGTVLPSAAGLLGAAASIGTPVALVVAP
                     AAVSGAAAERAAELGAARVLVADRPAGSPLLAPVLDALIAAAASVSPDAVLISSSSEG
                     RDVAARFAVRSGAALAVDVVSVSRDEEGPVAHHSVYGGAYLVDSAATSGTLVATVRQG
                     AIEARAAAQTPSIEQLDVTPSGKRDVEVIDYQATVTASERPELRGAKTVVSGGRGVGS
                     AENFALVEQLADALGAAVGASRAAVDAGYVPASYQVGQTGVTVSPRLYIAVGISGAIQ
                     HRAGMQTAKTIVAINKDADAPIFEIADFGVVGDLFTVVPQLIDAISAKKD"
     gene            complement(1222..2001)
                     /locus_tag="PLW15_10235"
     CDS             complement(1222..2001)
                     /locus_tag="PLW15_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT34811.1"
                     /translation="MKIVVLVKEVPDTWGDRRLNLETGLLERGAGELVLDEIGERAIE
                     VALTYAEANSGSEVVVLAMGPASVPTNLRKGLAMGADRAIHILDDALIGADTTLTATT
                     LAAALSHEGFDLVIAGNVSTDGGGAVIPSMVAELLDVPAATGLSTVAITATDVTGRRD
                     TEVGSYTVKAALPAVVSITEALPDARFPSFKGIMAAKKKPLDTLTLADLAATSGASGE
                     DAHSIVISLAERPARAAGVTIVDEGDAGTQLAEFLAKNQLI"
     gene            complement(2139..2897)
                     /locus_tag="PLW15_10240"
     CDS             complement(2139..2897)
                     /locus_tag="PLW15_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34812.1"
                     /translation="MATTTKPRTTKAAAKGSSTTASTAKIETDERAESRWPALAAILV
                     VLALYALLPSTFPLWVRLVIVFAAIVLVIPSLVANPLNQSSQKPWSRPLALALTGLLA
                     VANEVDLVLLIGRLVQPADAADAPMLLLSAVQVWSMNVIVFGLIFWQLDRGGPVTRTQ
                     APRDELPEADFRFPQDEDHDSVVEVAAGSSGRTDWTANFIDYLYFSLSNAMAFSPPDA
                     VPLLNRTKILTGIEALGAYVLLVLVIARAVSLLG"
     gene            complement(3037..3112)
                     /locus_tag="PLW15_10245"
     tRNA            complement(3037..3112)
                     /locus_tag="PLW15_10245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3077..3079),aa:Ala,seq:cgc)
     gene            complement(3278..>3481)
                     /locus_tag="PLW15_10250"
     CDS             complement(3278..>3481)
                     /locus_tag="PLW15_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34813.1"
                     /translation="RPVIEPVEIGDPTEALAAAAAQAEEALRSAPQPPAIPSRFAAMG
                     RVDAAGTPLPDLDAALARRRHAG"
BASE COUNT          532 a         1173 c         1259 g          517 t
ORIGIN      
        1 gttgcggccg cgggagttga agcggcgggc ttagcgttgg caccgagccc ggtgcgctgc
       61 gggcgcgggg cggcgggagg tggtgtaggg gcggtgtccg cggtcggtgc gggcgcggca
      121 tccggtgcgg gctggccagc gaccggggtg gacgcgccct gtcccggagc gcccgcgccc
      181 tgagcgccgg caccctggcc cggacgggga agcccctcgc gtgccatcgg ctagtccttc
      241 ttcgcggaga tcgcgtcgat gagctgcggc accaccgtga acaggtcgcc cacgacgccg
      301 aagtcggcga tctcgaagat cggggcgtcg gcatccttgt tgatggcgac gatcgtcttg
      361 gcggtctgca tgccggcccg atgctggatc gcgcccgaga tgcccaccgc gatgtacagg
      421 cgcggcgaca cggtcacgcc ggtctggccg acctgatacg aggcgggaac gtagccggca
      481 tcgaccgcgg cccgggatgc cccgaccgca gcgccgagcg cgtcggccag ttgctcgacc
      541 agcgcgaagt tctcggccga acccaccccg cggccacccg agacgacggt cttcgcgcct
      601 cgcaactcgg gacgttcgct cgcggtcacg gtcgcctggt agtcgatgac ctcgacgtcg
      661 cgcttgccgc tgggcgtgac atccagctgc tcgatgctcg gcgtctgggc cgcggcgcgc
      721 gcctcgatgg caccctgccg caccgtcgcg acgagggttc cggatgtcgc agccgagtcc
      781 acgaggtacg ccccgccgta cacggagtgg tgggcgaccg gcccctcctc gtcgcgcgag
      841 acggacacca cgtccaccgc aagagcggca ccggaccgca ccgcgaaccg ggccgcgacg
      901 tcgcgaccct cgctcgagct ggagatgagc accgcgtcgg gactcacgct ggctgcggcg
      961 gcgatcaggg catcgaggac cggcgccagg agtggcgatc ccgcgggccg gtcggcgacc
     1021 agcacacgtg cggcgccgag ctcggcggcg cgttcggctg ccgcgccaga cacggcggcg
     1081 ggagcgacca cgagcgccac gggggttccg atgctggcgg cagcgccgag cagtccggcc
     1141 gcgctgggga gcacggttcc ggcgggcgag acctcgacaa gaacgaggat gggtgcggag
     1201 gaatcagtca tggtgtcgtc ctcagatcag ctggttcttg gcgaggaact cggccagctg
     1261 cgtgccggcg tcgccctcat cgacgatggt gaccccggcc gcgcgagctg ggcgctcggc
     1321 gaggctgatc acgatggagt gggcgtcctc gcccgaggct cccgaggttg ccgcgaggtc
     1381 ggcgagcgtg agggtgtcca gcggcttctt cttggcggcc atgatgccct tgaagctggg
     1441 gaaccgcgca tccgggagcg cctcggtgat cgacacgacc gcgggcaggg ctgccttcac
     1501 ggtgtagctg cccacctcgg tgtcgcgtcg accggtcacg tcggtggcgg tgatcgcgac
     1561 tgtgctcagt cccgttgccg cgggcacatc gagcagctcc gcgaccatcg acgggatgac
     1621 ggcaccgccg ccgtccgtcg acacgttgcc cgcgatcacg aggtcgaagc cctcgtggct
     1681 gagcgcggcg gcgagagtgg tggcggtcag ggtggtgtcg gcaccgatga gcgcgtcatc
     1741 caggatgtgg atggcccgat ccgcacccat cgcgaggccc ttgcgcaggt tggtcggcac
     1801 cgacgccgga cccatcgcga gcacgacgac ctcgctgccg gagttcgcct cggcgtaggt
     1861 cagcgccacc tcgatcgcgc gctcgccgat ctcgtcgagc acgagttcac cggcgccgcg
     1921 ctcgagcagc ccggtctcca ggttcagccg gcgatcgccc caggtgtccg ggacctcttt
     1981 gaccagaacg acgatcttca tgtcgtgcct tcctatccgt cgaaccagta tcgccgtacc
     2041 cggcgcactc ctccagccag ttgggggctc tcgacaaccg cggcgcggct gaagccccac
     2101 cgatcaggga tgatcgacag cccgctctgg ctcggccgct accccagcag cgagacggcg
     2161 cgcgcgatca cgagcaccag gagtacgtag gcaccgagcg cctcgatccc ggtgaggatc
     2221 ttcgtgcggt tcagcagcgg caccgcgtcg ggtgggctga acgccatggc gttcgagagc
     2281 gagaagtaca ggtagtcgat gaagttggcc gtccagtcgg tgcgcccgga tgacccggcc
     2341 gccacctcca cgacgctgtc gtgatcctcg tcctggggga agcggaagtc cgcctcgggc
     2401 agctcatcgc gaggagcctg agtgcgggtc acgggaccgc cccgatcgag ctgccagaag
     2461 atgaggccga agacgatcac attcatcgac cagacctgca ccgccgagag gaggagcatc
     2521 ggggcatccg cggcatcggc cggttgcacc aggcggccga tgagaagcac caggtcgact
     2581 tcgttggcga ccgccaacag gcccgtgagg gccagtgcga gcggacgcga ccagggcttc
     2641 tggctgctct ggttcaacgg attcgccacc aacgacggaa tgacgagcac gatcgcggcg
     2701 aacacgatca ccagccgaac ccacaacggg aaggtgctcg gcagcagagc gtagagcgcg
     2761 agcaccacga ggatcgcggc cagcgccggc caccggctct cggcgcgctc gtcggtctcg
     2821 atcttcgcgg tcgaagccgt ggtgctcgag cctttcgcgg cggccttcgt cgttctcggc
     2881 ttggttgtcg tggccatcag cccatccttc gttcgcgcgt gcctgtgagt gcctcagaaa
     2941 ggctggcacg atgggcggaa ctgggcgaag ggcttgacaa cggcagtcga gtggcgtgcg
     3001 agagacaaga aaagccgccc gaaggcggct tcccggtggt ggaccctagg agatttgaac
     3061 tcctgacctc ctcgatgcga acgaggcgcg ctaccaactg cgccaagggc ccgtgatgcg
     3121 ttagctaggt tatcacctgc gcgcgagggg tttcgatacg cggcttcgcc gctactcaac
     3181 cggcgaaagc gggtgggttt cgatacgcgg cttcgccgct actcaaccgg cgaaagcggg
     3241 tgggtttcga tacgcggctt cgccgctact caaccggcta gccagcgtgg cggcggcggg
     3301 ccagcgcggc gtcgaggtcc gggagcgggg tgccagcggc atccactcgt cccatcgcgg
     3361 cgaagcggct cgggatcgcg ggcggctggg gcgccgaacg cagtgcctcc tcggcctgcg
     3421 cggctgcggc ggccagcgcc tcggtggggt caccgatctc gaccggctcg atcactggcc
     3481 t
//
LOCUS       DAOSOU010000218         3476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27150_length_3476_cov_6.009939, whole genome shotgun sequence.
ACCESSION   DAOSOU010000218 DAOSOU010000000
VERSION     DAOSOU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27150_length_3476_cov_6.009939"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLW15_10255"
     CDS             <1..473
                     /locus_tag="PLW15_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOT34814.1"
                     /translation="IIPRFGSDYTRPILEGVAVHGALADGIGHYPGTQMPGDVGNVAL
                     AGHRTGWGAPLGDIGNLMVGDSIYLETEDGWYKYIFRTFAYVMPTGVDVLAAVPQNPD
                     AAPTDRILTLTSCNPKTTAAERIIAYSIFDTWYPRAGGPPPELAPEVAAAAAAG"
     gene            473..634
                     /locus_tag="PLW15_10260"
     CDS             473..634
                     /locus_tag="PLW15_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34815.1"
                     /translation="MYAALWRVLPGPWWVRALILAAAAAVVLTACVLWVFPWVNDFIT
                     PQDVTVEGQ"
     gene            631..1269
                     /locus_tag="PLW15_10265"
     CDS             631..1269
                     /locus_tag="PLW15_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297510.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT34816.1"
                     /translation="MTHVLVVDNYDSFVYTLNGYLLQLGAQTTVVRNDAFSADDAAQV
                     IRDYDAVLLSPGPGTPAAAGVSIPIVKAVLESGQPLLGVCLGHQAIGEAMGATVTHAE
                     ELMHGKTSLITHDSSAFYDGVPQPFVATRYHSLAIVDDTVPSDLIVTSRTEGGVIMGV
                     RHESLPIYGVQFHPESVLTEGGYRMLGNWLAVAGLPEAAETARGLTPLVKLA"
     gene            complement(1348..3069)
                     /gene="pknB"
                     /locus_tag="PLW15_10270"
     CDS             complement(1348..3069)
                     /gene="pknB"
                     /locus_tag="PLW15_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOT34817.1"
                     /translation="MSEGVAQGVRLLAGRYELGELIGRGGMADVHVGMDTRLGRRVAV
                     KLLKPSLANDPAFRTRFRREAQDAAKMAHPTIVRIFDAGEESIRESNGAETLVPFIVM
                     EYVDGRLLKDVLADGPLEPAEATRIISQVLTALEYSHRAGVVHRDIKPGNIMITSSGQ
                     VKVMDFGIARAISESSATIAESSAIVGTAQYFSPEQARGESVDARTDLYSTGIVLFEL
                     LTGRAPFRGDNPVAVAYQHVNSDPVAPSTLNPRVSPALDAVVLRALAKDRFSRYQSAG
                     EFRADVEAAAAGEVPARTQLVANDFNATLFGVNPSSVAGSEATLRQLTVDNDERLVRT
                     QSRPPVAWIWAGIASMVVIIAAVIFWTFNLAPAQLTANVAVQVPDVVGQTYADGSASL
                     IDAGLVPEKVAQTSDTVPEGAIISTDPAAGITVNPGLTVTVYVSLGETPVTVPNVANM
                     TEADAIAALQAKGLKYGSTTQTYSASVRQGVVIDSDPPGGSERVGSEVIRIGHEVNLV
                     VSNGLVQVPDVTGKSIAEASSALNAIGLNPTVQVDQGCSGGTVASQSIVGDQPQKSPI
                     SLRYCGG"
     gene            complement(3138..>3476)
                     /locus_tag="PLW15_10275"
     CDS             complement(3138..>3476)
                     /locus_tag="PLW15_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOT34818.1"
                     /translation="KGETITVNFYTEIPPPSAPKPAAVTAPAGPWLAGDDITVNWPNY
                     SGCPSGHPLSGFNFQITNGTAASTNPISSSATTLSIELGTPGTTTIAYTALCTDFESL
                     VSDQTSVTVN"
BASE COUNT          555 a         1157 c         1170 g          594 t
ORIGIN      
        1 tcatcattcc gcgattcggc tccgactaca cccgtccgat cctcgagggc gtcgcagtgc
       61 acggagcact cgccgacggt atcggtcact accccggcac gcagatgccc ggagacgtcg
      121 gcaatgtcgc tctcgccggt caccgcaccg ggtggggagc acccctgggc gacatcggca
      181 atctgatggt cggcgacagc atctacctcg agaccgagga cggctggtac aagtacatct
      241 tccgcacgtt cgcctacgtc atgccgacgg gcgtcgatgt gctggccgca gttccgcaga
      301 atcccgacgc ggccccgacc gatcgcatcc tcaccctcac cagttgcaac cccaagacga
      361 ccgcggccga gcgcatcatc gcctacagca tcttcgacac gtggtacccg cgagcgggcg
      421 gcccgcctcc tgagctcgcc cccgaggtcg cggccgcagc ggcggccggc tgatgtacgc
      481 ggcgctctgg cgagttctac ccggcccgtg gtgggtgcgc gcgctcatcc tggcggccgc
      541 cgccgctgtc gtgctcaccg cttgtgtact ctgggtgttc ccgtgggtga acgacttcat
      601 caccccgcag gacgtcaccg tggaggggca atgacacacg tactcgtcgt cgacaactac
      661 gacagtttcg tctacacgct caacggctac ctcctgcagc tcggtgcgca gaccacagtg
      721 gtgcgcaatg atgccttctc agcggatgac gccgcgcagg tcatccgcga ctacgacgca
      781 gtgctgctgt cgccaggccc cggtactccg gccgcagcgg gagtgagcat cccgatcgtg
      841 aaggccgtgc tcgaatccgg ccagccgctg ctcggagtgt gcctcggaca ccaggcgatc
      901 ggtgaggcga tgggcgccac tgtcacgcac gccgaggaac tgatgcacgg caagacctcg
      961 ctgatcactc acgactccag cgccttctac gacggagtac cccagccctt cgtcgccacc
     1021 cgctaccact cgctcgccat cgtcgacgac accgtgccga gtgacctcat cgtcacgtcg
     1081 cgcaccgagg gcggcgtgat catgggcgta cgccacgaga gcctgccgat ctacggtgtg
     1141 cagttccacc ccgagtcggt gctcaccgag gggggctatc ggatgctcgg caactggctt
     1201 gccgtcgccg gcctgcccga ggctgccgag accgcgcgcg gactcacgcc gctcgtcaag
     1261 ctcgcctagg gtttcgatac gccgcttcgc ggctactcaa ccagcgggag gcgccgcttc
     1321 gcggctactc aacccgctgg aggcggatca gccgccgcag tagcgcaggc tgatcggtga
     1381 cttctgcggc tggtcgccca cgatggactg gctggcgacc gtgccgccgg agcagccctg
     1441 gtccacctgc acggtggggt tgagtccgat ggcgttgagc gcgctcgagg cctccgcgat
     1501 cgacttgccc gtgacatccg gaacctgcac cagaccgttg gaaacgacca ggttcacctc
     1561 gtggccgatg cggatgacct cggaaccaac ccgctccgag ccgccgggag gatcggagtc
     1621 gatcacaact ccctgccgaa cgcttgccga gtaggtctga gtggtgctgc cgtacttgag
     1681 tcccttggcc tggagtgctg cgatcgcatc cgcctcggtc atgttcgcca cattgggcac
     1741 ggtgaccggg gtctcaccga gggacacgta gacggtgacg gtgagtcccg ggttgaccgt
     1801 gatgccggcg gcggggtcgg tgctgatgat cgcaccctcg ggaacggtgt cgctggtctg
     1861 agcgaccttc tcgggaacca gcccggcgtc gatgagcgag gccgacccgt cggcgtaggt
     1921 ctggccgacc acatcgggca cttgaacggc aacgttggcc gtcagctggg cgggggcgag
     1981 gttgaaggtc cagaagatca cggcggcgat gatcacgacc atgctcgcga tgcccgccca
     2041 gatccacgcg accgggggac gactctgcgt gcgcaccagt cgctcatcgt tgtcgacggt
     2101 gagctggcgc aaggtcgcct cggagccggc gaccgaactc gggttcaccc cgaacagggt
     2161 cgcattgaag tcattcgcga ccagctgcgt gcgcgcggga acctcgccgg cggccgcagc
     2221 ctcgacatcg gcacggaatt cgcccgcgct ctggtagcgc gagaagcgat ccttggccag
     2281 cgccctgagc accacggcgt cgagcgccgg cgacaccctg gggttgagcg tgctcggagc
     2341 gaccggatcg gagttcacgt gctggtaggc caccgcgacc gggttgtcac cgcggaacgg
     2401 cgcgcgcccg gtgaggagct cgaagagcac gatgccggtg gagtacaggt cggtgcgggc
     2461 atccacgctc tcgccgcggg cctgctcggg ggagaagtac tgggcggtgc cgacgatcgc
     2521 gctcgattcg gcgatcgtgg cggatgactc ggagatcgcc cgcgcgatgc cgaagtccat
     2581 aaccttcacc tgcccgctcg aggtgatcat gatgttgccg ggcttgatgt cgcggtgcac
     2641 gacaccggcg cggtgcgagt actcgagcgc cgtcagcact tggctgatga tgcgggtcgc
     2701 ctcggcgggt tcgaggggac cgtcagcgag cacgtccttg agcaggcggc cgtcgacgta
     2761 ctccatcacg atgaacggga cgagcgtctc ggctccgttg gactcgcgga ttgactcctc
     2821 gccagcgtcg aagatgcgca cgatcgtggg atgcgccatc tttgctgcgt cctgcgcctc
     2881 gcgacggaag cgggtgcgga aggctggatc gttcgcgagg gagggcttga gcagcttgac
     2941 cgcaacgcgg cggcccaacc gagtgtccat tccgacgtgg acgtcggcca tgccgccacg
     3001 cccgatcagc tcgccgagct catagcggcc ggcgagcagg cgcacgccct gtgcaacacc
     3061 ttcgctcacg cgtactctcc ccggtcaaac ccgacagtat ctctcagagt gtacccggtg
     3121 ggttctgcgc cacagtgtca gttgactgtg actgacgtct ggtcggacac caacgattcg
     3181 aagtcggtgc agagcgccgt gtaggcgatc gtggtggttc ccggggttcc cagctcgatc
     3241 gacagggtcg ttgccgacga cgagatcggg ttggtgctgg ccgccgtgcc gttggtgatc
     3301 tggaagttga atccgctcag ggggtgtcca ctggggcagc cgctgtagtt cggccagttc
     3361 acggtgatgt cgtcgccagc gagccacggc ccggccggtg cggtgaccgc ggccggcttg
     3421 ggtgccgagg ggggcggaat ctccgtgtag aagttgaccg tgatcgtctc gccctt
//
LOCUS       DAOSOU010000219         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27181_length_3474_cov_7.090962, whole genome shotgun sequence.
ACCESSION   DAOSOU010000219 DAOSOU010000000
VERSION     DAOSOU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27181_length_3474_cov_7.090962"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLW15_10280"
     CDS             <1..887
                     /locus_tag="PLW15_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34819.1"
                     /translation="ELMVARMLVPSSDDTERGALARVERLERRIGVEQDAAAPVARVA
                     LAKQVVEPVETPPAQTPPAKTPPAKTPEAAIPEAPVSTSSTTERPAAEAPVSTSSTTG
                     DSATAPVAAKAAGEVTLEQLRNAWPEVLESVQKTKMSAWAVVYTATARALDGDVLTLS
                     FVSQADVDSFKQPQGSGEGVSEVLRRAIVEVLGLRVKFIAKADASAEPPAAAPTTSVT
                     PPVSEKDAQGWAVAEIPGAEPPAPAPAAKASAASSEPAKPADKARYGESVVRELLGAS
                     FIEEQDVAPRVVPRPADD"
     gene            887..1486
                     /gene="recR"
                     /locus_tag="PLW15_10285"
     CDS             887..1486
                     /gene="recR"
                     /locus_tag="PLW15_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT34820.1"
                     /translation="MYDGIVQELIDELGRLPGIGPKSAQRIAFHILQTESFDVTRLSE
                     VLRDVRDKVRFCDICGNVTEEQTCSICRDPRRSAATICVVEEAKDVVAIERTREFKGL
                     YHVLGGAISPIDGIGPDDLRIRQLLTRLNDGTVTEVIIATDPNLEGEATATYLTRLLV
                     QPGLRVTRLASGLPVGGDLEYADEVTLGRAFEGRRLVEN"
     gene            complement(1483..1965)
                     /locus_tag="PLW15_10290"
     CDS             complement(1483..1965)
                     /locus_tag="PLW15_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34821.1"
                     /translation="MTLEAWTDFNVAVAGATAALAGLLIVALSVNVGEIVKAPTLTAR
                     AASAISTLLLGLVATASGLIPSQPLWVLGLEIALATIVAWVLQVHAMVALGRDGRTTS
                     EHLAKSLVGIVPLAGFSVGSVMLLLGMPGGLYAVAAGCVLAIVAAVIFSWVALVEVLR
                     "
     gene            2045..3319
                     /locus_tag="PLW15_10295"
     CDS             2045..3319
                     /locus_tag="PLW15_10295"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT34822.1"
                     /translation="MSLIVQKFGGSSVADAESIKRVAKRIVETRKAGNDVVVAVSAMG
                     DTTDELIDLAHEVTPMPDPREMDMLLTTGERISMALLAMAIKSMGVDARSYTGSQAGM
                     ITDARHGSARIVDVTPTRLREALDEGAIVIVAGFQGFNRDTKDITTLGRGGSDTTAVA
                     LAAALGADVCEIYTDVDGIFTADPRVVPRAHKIDRVTSEEMLELAAAGAKVLYIRAVE
                     YARRHGVTLHVRSSFNNNEGTWVVDSYDTDGGVAVEEPIITGVAGDLNEAKITVVGVP
                     DVPGKAAEIFTVVASTGANIDMIVQNVSAASTGLTDISFTLPKVEGEKVLTALRAGQE
                     SAGFQSLQYDDQIGKLAVVGAGMRTSAGVSAQLFRALSDAGINIEMISTSEIRISVVT
                     RADMLNEAMRVVHTAFGLDGETEAVVHGGTGR"
BASE COUNT          597 a         1184 c         1189 g          504 t
ORIGIN      
        1 tcgagctcat ggtcgcgcgc atgctcgtgc cgtcgagcga cgacaccgaa cgcggcgcgc
       61 ttgcccgtgt cgagcgtctc gagcggcgta tcggtgtcga gcaggatgcc gcggctcccg
      121 tcgcccgcgt agcgctcgcg aaacaggtgg tcgagcccgt cgagaccccg cccgcgcaga
      181 ccccgcccgc gaagaccccg cccgcgaaga cccccgaggc agcgatccct gaggcgccgg
      241 tctcgacgag ctcgaccacc gaacgtccgg ccgccgaggc accggtctcg acaagctcga
      301 ccaccggaga ctcggccacc gcacccgtcg ccgcgaaggc cgcgggcgag gtcaccctcg
      361 agcagctgcg caacgcctgg cccgaggtgc tcgagtccgt gcagaagacg aagatgagcg
      421 cgtgggccgt cgtctacacg gcaacggccc gtgcccttga cggcgatgtg ctgaccctga
      481 gcttcgtgag ccaggccgac gtggatagct tcaagcagcc gcaaggttcc ggcgagggcg
      541 tgagcgaagt tctgcgtcga gcgatcgtcg aggtgctcgg cctgcgggtg aagttcatcg
      601 cgaaggccga tgcgtcggcc gaaccgcccg ccgcggcgcc gaccacgagt gtgaccccgc
      661 ccgtatccga gaaggatgcg cagggctggg cggttgccga gatccccggc gccgagccgc
      721 cagcgcccgc acccgcggcg aaggcttcgg cggcgtcatc cgagcccgcg aagcctgccg
      781 acaaggctcg ctacggtgag tcggtggtgc gtgagcttct cggcgcgagc ttcatcgagg
      841 agcaggacgt cgctccccgc gtcgtgccgc ggccggcgga cgactgatgt acgacggaat
      901 cgttcaggag ctcatcgacg agctcggccg tctgcccggt atcggcccga agtcggctca
      961 gcgcatcgcc ttccacatcc tgcagacgga gagcttcgac gtcacccgcc tgtccgaggt
     1021 gttgcgcgat gtgcgggaca aggtgcgctt ctgcgacatc tgcggcaacg tgaccgagga
     1081 gcagacctgt tcgatctgcc gtgacccgcg gcgttcggcg gccaccatct gcgtggtcga
     1141 ggaggcgaag gatgtcgtcg ccatcgagcg cacccgcgag ttcaagggcc tgtaccacgt
     1201 gctcggcggc gcgatcagtc cgatcgacgg catcggcccc gacgacctgc gcatccgcca
     1261 gctgctgacc cgcctcaatg acggcacggt caccgaggtg atcatcgcga ccgacccgaa
     1321 cctcgagggt gaggcgacag cgacctacct cacgcgactg ctcgtgcagc ccgggctccg
     1381 ggtcacccgc ctcgcgtcgg gcctgccagt gggcggcgac ctcgagtacg ccgacgaggt
     1441 tactctgggc cgcgccttcg agggccgacg gctcgtcgag aactagcgca gcacctcgac
     1501 gagggccacc cacgagaaga tcacggcggc cacgatggcc agcacgcagc cggcggccac
     1561 ggcgtagagg cctccgggca tcccgagcag cagcatcacg ctgccgacgc tgaagccggc
     1621 cagtggcaca attcccacga gcgacttggc gaggtgctca gaggtcgtcc ggccgtcgcg
     1681 acccagcgcg accatcgcgt gcacctgcag cacccaggcc acgatcgtcg cgagcgcgat
     1741 ctccagcccg agcacccaga gcggctgact ggggatcagt cccgacgcgg tggcgacgag
     1801 gccgagcagg agggtcgaga tcgcgctggc ggcccgggcg gtcagggtgg gggccttcac
     1861 gatctcgccc acgttgaccg agagggccac aatgagcagt cccgcgaggg ccgcggtggc
     1921 accggccacg gcgacattga agtccgtcca tgcctcgagc gtcatgcaca acagggtagc
     1981 gccagcgagc acctagaatt ggtggtcggg ccaagcccga attccccagt tccaggagcc
     2041 acacgtgagc ttgatcgtcc agaagttcgg cggttcgtcg gtcgccgacg ccgagagcat
     2101 caagcgtgtc gccaagcgca tcgtcgagac gcgcaaggcc ggaaacgacg tcgtcgttgc
     2161 ggtcagtgcg atgggcgaca cgaccgatga actcatcgac ctcgcccacg aggtgacccc
     2221 gatgcccgac ccccgcgaga tggacatgct gctcaccacc ggcgagcgca tctcgatggc
     2281 tctgctggcc atggcgatca agagcatggg cgtcgatgcc cgctcgtaca ccggcagcca
     2341 ggccggcatg atcacggatg cccggcacgg ctccgcccgc atcgtcgatg tcaccccgac
     2401 tcgcctgcgc gaggctctcg atgagggcgc gatcgtcatc gtcgcagggt tccagggttt
     2461 caaccgcgac accaaggaca tcacgactct cggccgcggc ggatcggaca cgaccgccgt
     2521 cgccctggct gccgccctcg gtgctgatgt ctgcgagatc tacaccgacg tcgacggcat
     2581 cttcactgct gacccgcgcg tggttccccg cgcacacaag atcgatcgcg tcaccagcga
     2641 ggagatgctg gagctcgcgg cggcgggtgc gaaggtgctg tacatccgcg cggtcgagta
     2701 cgcccgccgg cacggggtga cgctgcacgt gcggtcatcc ttcaacaaca acgaaggcac
     2761 ctgggtcgtt gactcgtacg acactgacgg aggagtagcc gtggaagaac cgatcatcac
     2821 gggcgttgcg ggcgacctga acgaagcgaa gatcaccgtc gtgggtgttc ccgacgtgcc
     2881 aggcaaggcg gcggagatct tcacggtcgt ggcctcgacc ggggccaaca tcgacatgat
     2941 cgtgcagaac gtgtctgccg catccaccgg gctcaccgac atctcgttca cgctgccgaa
     3001 ggtcgagggg gagaaggtgc tcaccgcgct gcgggcgggt caggagtcgg ccgggttcca
     3061 gtcgctgcag tacgacgacc agatcggcaa gctcgccgtg gtgggcgccg ggatgcgcac
     3121 gagtgccggt gtttcggcgc agctgttccg cgcactctcc gacgcgggca tcaacatcga
     3181 gatgatctcg acgagcgaga tccgcatctc ggttgtgacg cgcgccgaca tgctcaacga
     3241 ggcgatgcgg gtcgtgcaca ccgcgttcgg cctcgatggt gagaccgagg cggtcgtgca
     3301 cggcggcacc gggcgctgac gccttcgccg gttgagtagc ggcgaagccg cgtatcgaaa
     3361 ccgcggtgtg gcagcatctc gacggccgct gacggctcac tccgccgagc ctgcgccgtg
     3421 agcatgccac aattgttccc atgggtaacg gtgtgagaat cggcgtcgtg ggcg
//
LOCUS       DAOSOU010000220         3453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27405_length_3453_cov_3.251913, whole genome shotgun sequence.
ACCESSION   DAOSOU010000220 DAOSOU010000000
VERSION     DAOSOU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27405_length_3453_cov_3.251913"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..343
                     /locus_tag="PLW15_10300"
     CDS             173..343
                     /locus_tag="PLW15_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34823.1"
                     /translation="MTKDSPTTPSLQTEPVMDGAMDHEGPPPMKDSDGNTVDLTSPTD
                     EEPTEDEPGAAG"
     gene            complement(340..546)
                     /locus_tag="PLW15_10305"
     CDS             complement(340..546)
                     /locus_tag="PLW15_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34824.1"
                     /translation="MNSSAARFALTIAGALALLMGLIFAGQGANLIPGSFMTGDSTWL
                     VVGIILAVIGVVLLVVALRRRPRS"
     gene            complement(594..1415)
                     /locus_tag="PLW15_10310"
     CDS             complement(594..1415)
                     /locus_tag="PLW15_10310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT34825.1"
                     /translation="MTRPRLALVTGAGGGIGLGVARMLRERGYRIVAVDISDAAAASA
                     AERIGGGAIPVACDASDRAQVAALADRIRGEWSAELDVLVCNAGIIVPGTVAESAPDA
                     LTLQLDVMLGFPVQLINAAVPAFVARGNGHVLATVSMGGILSLPGSAVYSAAKSGLRA
                     FLTALSAELAGSGVAVSGIYPSAVDTAMLRHEARHGGSMLNFLGTVFTVDDVVKGYER
                     ALRTRRLEVYLPYIDSVTSRLAHFWPGASNRLLPVLDRLGKRGHAKYLARIDAEG"
     gene            complement(1402..2748)
                     /locus_tag="PLW15_10315"
     CDS             complement(1402..2748)
                     /locus_tag="PLW15_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOT34826.1"
                     /translation="MVDPTALPVVIVGAGPAGLAAARALALRDIDYVQLERHTDVGGI
                     WDIDNPGTPMYKSAHFISSRDKSGFYDYPMPASFADYPSRTQILDYTRAFADEFGLRQ
                     RIRFGTSVTSVTEDSGVWTVHTTDGTIEARAVIACSGVTWDARMPDVPGTFDGEIIHS
                     VSYRDTSQFAGKRVLIVGLGNSGADIACDAAASADTAYISTRRGYHFIPKHILGEPSD
                     NTEWLPIWGERLLYNIMKPIVIGDVRRWGLPKPDHKLFESHPLLNTQLLHYLQHGDIT
                     AKPGIERFDGPDVVFTDGSRAQIDLVVFATGYDMSIPYVPDDYVDWKSHRPDLYLNVF
                     SRRKDDLFGVSYIEVNSSAYTLFDHIANLIAQYLVDERDRPQAAERFRKLVQHDRPDL
                     SGGIHFVATDRHATYVEVRAFRKQLRRIARLLGWSTLAPGMFDRLRRSTGAARDAA"
     gene            2828..3424
                     /locus_tag="PLW15_10320"
     CDS             2828..3424
                     /locus_tag="PLW15_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT34827.1"
                     /translation="MPKATRSALVEAALRVIERDGVAAATTRQIADEASAPLGTVHYW
                     FADKDELLRAVVDSLLTQVRADVHRASEHAGSAERLTGAYEAFMAMPVGRQLALFELT
                     TFAIRNDRLRQVAADQYQAYRAAAQEGLQPWREAAEALPGGADALGALLVAVIDGLTL
                     ASLADPDSRGVEALGLFAHLLLLAGVGGEVIAEATTPS"
BASE COUNT          548 a         1061 c         1279 g          565 t
ORIGIN      
        1 cacggtcggg cgatcggcat cgtcgtgtcg ctcgcgaccg ccgcctacct gctggcgcgg
       61 gggttcgggc tgttctagcg gccgtctaag gcctggcagg gcgctctcaa cccctaacga
      121 tccaggccgt cgaggcgcac cctgaggtaa caacacgaac gagggacgcg ccatgacgaa
      181 ggactcaccc acaacgccca gcctgcagac ggagcccgtg atggacgggg cgatggacca
      241 cgaggggccg ccgccgatga aggacagcga cgggaacacc gtcgatctga cctcacccac
      301 cgacgaagag ccgaccgagg atgagcctgg cgcggccggc tagctacgcg ggcgccgccg
      361 cagtgcaacg acgagcagca cgacgccgat caccgcgaga ataatgccga ccacgagcca
      421 ggtcgagtcg ccggtcatga agcttccggg gatcaggttt gctccctgcc ccgcgaagat
      481 caggcccatg agcagcgcga gcgctccggc gatggtgagg gcgaagcgtg cggcgctact
      541 gttcatggcc ccacgctaca gctccgacac tgccgccgtc cggggtcggt gagctaacct
      601 tccgcgtcga tgcgcgcgag gtacttcgcg tggccgcgct tgccgagccg gtcgagcacg
      661 ggaagtagtc ggttggatgc tcccggccag aagtgggcga ggcgcgaggt gacgctgtcg
      721 atgtagggca agtacacctc caggcgccgg gtgcgcagcg ctcgctcgta acctttgacg
      781 acgtcgtcga cggtgaagac ggtgccgagg aagttgagca tgctgccccc atgccgcgcc
      841 tcgtggcgca gcatcgccgt gtcgacggcg ctggggtaga tgccactcac cgcgacaccg
      901 ctgccggcga gttcggccga gagggctgtg aggaacgccc gaagacctga cttcgcggcc
      961 gagtacacgg ccgacccggg cagcgacagg atgccgccca tcgacacagt ggcgagcacg
     1021 tgtccgttgc cgcgcgccac gaaagcgggc accgctgcgt tgatgagttg cacggggaag
     1081 ccgagcatca catccaattg cagggtgagg gcgtcggggg ctgactcggc caccgttccg
     1141 ggaacgatga tgcccgcgtt gcacacgagc acgtcgagtt cggcgctcca ctcaccgcgg
     1201 atgcggtcgg cgagggcggc gacctgggcg cgatcgctgg cgtcgcaggc gaccgggatg
     1261 gcgccgccgc cgatgcgttc ggcggcgctg gcggctgcgg catccgagat gtcgacggcg
     1321 acgatgcggt agccgcgctc gcgcagcatg cgggccacac cgaggccgat gccaccgccg
     1381 gccccggtga cgagggcgag tctaggccgc gtcacgtgct gctcccgtcg atcggcgtag
     1441 gcggtcgaac atgccgggcg cgagcgtgct ccagccgagc aggcgggcga tgcgccgcag
     1501 ctgcttgcgg aatgcgcgca cctcgacata cgtggcgtgc cggtcggtgg cgacgaagtg
     1561 gatgcctccg ctcaggtcgg gccggtcgtg ctgcaccagc ttcctgaacc gttccgccgc
     1621 ctgcggtcgg tcgcgttcgt cgacgaggta ctgcgcgatg aggttggcga tgtggtcgaa
     1681 gagtgtgtag gccgacgagt tgacctcgat gtagctcacg ccgaacaggt cgtctttgcg
     1741 gcggctgaac acgttgaggt agaggtcggg ccggtggctc ttccagtcga cgtagtcgtc
     1801 gggcacgtag gggatcgaca tgtcgtaccc ggtggcgaac acgaccaggt cgatttgcgc
     1861 gcggctgccg tcggtgaaga ccacgtcggg tccgtcgaag cgttcgatgc cgggtttcgc
     1921 ggtgatgtcg ccgtgctgca ggtagtgcag cagctgggtg ttgagcagcg ggtgagattc
     1981 gaacagtttg tggtcgggtt tgggcagccc ccagcgccgc acgtcgccga tgacgatggg
     2041 tttcatgatg ttgtagagca ggcgttcacc ccagatgggc agccactcgg tgttgtctga
     2101 cggttcgccg aggatgtgtt tgggaatgaa gtggtacccg cgcctggtgc tgatgtaggc
     2161 ggtgtcggcg gatgctgctg cgtcacatgc gatgtcggcg cccgagttgc cgaggccgac
     2221 gatgagcacg cgcttgcccg cgaactggga cgtgtcgcgg tagctgacgg agtggatgat
     2281 ctcgccgtcg aacgtgccgg gtacgtcggg catgcgggca tcccaggtca ccccgctgca
     2341 ggcgatgacg gcacgggcct caattgtgcc gtcggtggtg tggacggtcc agacgcctga
     2401 gtcttcggtg accgatgtca cgctggtgcc gaagcggatg cgttggcgca gcccgaactc
     2461 gtcggcgaat gctctggtgt agtcgaggat ctgggtgcgg ctggggtagt cggcgaagct
     2521 ggcgggcatc gggtagtcgt agaagcctga cttgtcgcgc gaggagatga agtgcgccga
     2581 cttgtacatg ggggtgccgg ggttgtcgat gtcccagatg cctcccacgt cggtgtggcg
     2641 ctcgagctgc acgtagtcga tgtcacgcag cgcgagggca cgggcggcgg cgaggccggc
     2701 ggggccggcg ccgacgatga cgacgggaag tgcggtggga tcaaccatga ttctcctcac
     2761 gtgggtgtgc gcgccagcat atctggtcat ttgaccaaat acgagtaccg gatgcgacaa
     2821 tgggactgtg ccaaaagcaa cccggtccgc gctcgtcgag gccgccctcc gcgtgatcga
     2881 acgcgacggg gtggccgccg cgacgacgcg acagatcgcg gatgaggcat ccgcgcccct
     2941 gggcaccgtg cactactggt tcgccgacaa ggatgagctg ctgcgggccg tggtcgattc
     3001 actgttgacc caggtgcgcg cggacgtgca ccgcgccagt gagcatgcgg gttcggcgga
     3061 gcgcctgacc ggggcgtacg aagcgttcat ggcgatgccg gtcgggcgcc agctcgcgtt
     3121 gttcgagctc actactttcg cgatccgcaa tgatcggttg cggcaggtgg ccgccgatca
     3181 ataccaggcc taccgggctg cggcgcagga aggtctgcag ccgtggcgcg aagccgccga
     3241 ggcactgccc ggcggggccg atgcgctcgg ggcccttctc gtggcggtga tcgacgggct
     3301 cacgctcgcg agcctcgccg accccgactc gcgcggtgtc gaggccctcg ggctattcgc
     3361 gcatctgctg ctgctcgccg gggtcggcgg cgaggtcatt gcggaggcga cgacgccgag
     3421 ttgacggctc cgacacttcc gccgtccggg tcg
//
LOCUS       DAOSOU010000221         3447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27482_length_3447_cov_5.332842, whole genome shotgun sequence.
ACCESSION   DAOSOU010000221 DAOSOU010000000
VERSION     DAOSOU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27482_length_3447_cov_5.332842"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3447)
                     /locus_tag="PLW15_10325"
     CDS             complement(<1..>3447)
                     /locus_tag="PLW15_10325"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HOT34828.1"
                     /translation="APAATEPPAAPPAVPGDPIRGVGKKIVENMEKSLSVPTATSFRE
                     VPARLLEVNRSVINGYLGRTRGGKVSFTHIIGYAVVRAIADHLPRMNNAYVEGPDGEP
                     RILQDRPVNLGLAIDQEKKDGSRSLIVAAIKGADQMDFKNFIATYDEIIRKANNNKLT
                     PDDFAGVTVSLTNPGTIGTERSVPRLMPGQGLIVGVGSLAYPTGFAAADPRTIADLGI
                     SKTMTISSTYDHRIIQGAESGLFLKKVHDMLLGGDRFYEDIFAAIGVPYTPVQWHTDD
                     NPVDREESAVTKQMKVSNLINMYRVRGHLIADLDPLAANPPQMHPELDPAFYGLTIWD
                     NDREFLTGFEAGVYAPVGGKERMKLGDILGVLRDAYCRTSGIEYTHISSIEEKRWIQE
                     QVEGASTKLDPTEQRHILDRMNAAEAIEKFLATKWMGQKRFGLEGAESTIAILDAILD
                     RSADQQMAGAVLGMAHRGRLNVLVNIVGKSYGQLFKEFEGHVDPDSIQGSGDVKYHLG
                     QTGTFTARSGNTIPVELAANPSHLEAVNPVVEGMVRAKMDNIPEGATQRFPVLPVLIH
                     GDAAFAGQGVVAETLNLSLIKGYRVGGTIHLIINNQLGFTTAPAAARSSEYCTDVAKT
                     VQAPIFHVNGDDPEACVRVARLALAYRQRFNKDVVIDMVCYRRHGHNEGDDPSYTQPI
                     MYRQIDARRSVRKLYTEALVRRGDISLDEAELFLADFNARLQAALEETRQSAPTEEVI
                     AKPHPPATGVLPKANTSVTKERLDEVYAALSAVPDGFTVHPKLVKQFETRTKMYHDGE
                     VDWALAEAFAFGTTLLEGTSIRLSGQDSRRGTFSHRHSTLVDYQNGDEFQPLSALATD
                     NTQLWIYDSLLSEYAVLGFEYGYSVTNPEALVIWEAQFGDFMNGAQIVIDQFIVSGED
                     KWHQTSGLVMLLPHGYEGQGPEHSSARIERFMNLAAEDNIQICNATTAAQYFHLLRRQ
                     MHRSVRKPLIVFTPKSGLRAKSYRSPVEQLITGTFEELLVDSSNIDPNSVTRLVLASG
                     KIGAEAAQHRDDIGATAAVARVEQLFPWPYEAVAKELARFPNCKEIVWLQEEPENMGP
                     WNGIKGRLFEAHGSEFEIRRISRSDSGSPATGKAAIHAQEQREILDRAFAPLTRRDWH
                     EF"
BASE COUNT          493 a         1059 c         1255 g          640 t
ORIGIN      
        1 tgaaactcgt gccagtcgcg gcgggtgagg ggagcgaagg cccgatcgag gatctcgcgc
       61 tgctcctgcg cgtggatcgc cgccttgccg gtggccggcg agcctgagtc ggagcggctg
      121 atgcggcgga tctcgaactc ggatccgtgc gcctcgaaca ggcgaccctt gatgccgttc
      181 caggggccca tgttctcggg ctcctcctgc aaccagacga tctccttgca gttggggaac
      241 cgcgccaact ccttggcgac cgcctcgtac ggccacggga acaactgctc gacgcgggcc
      301 actgcggccg tcgcgccgat gtcgtcgcgg tgctgcgcgg cctcggcgcc gatcttgccg
      361 gacgcgagca cgagtcgcgt gaccgagttc gggtcgatgt tcgagctgtc gacgaggagc
      421 tcctcgaagg tgcccgtgat gagctgctcg acgggggagc ggtacgactt cgcccgcagc
      481 ccggacttcg gtgtgaagac gatcagcggc ttgcggaccg agcggtgcat ctggcggcgg
      541 agcaggtgga agtactgcgc tgcggtcgtt gcgttgcaga tctggatgtt gtcctcggcg
      601 gcgaggttca tgaagcgctc gatgcgcgcc gagctgtgct cgggcccctg gccttcgtag
      661 ccgtgcggca ggagcatcac gagaccggag gtctggtgcc acttgtcctc gcccgacacg
      721 atgaactggt cgatgacgat ctgcgcgccg ttcatgaagt cgccgaactg cgcctcccag
      781 atgacgagcg cctcggggtt ggtcaccgag tagccgtact cgaagccgag caccgcgtac
      841 tcgctgagca gcgagtcgta gatccagagc tgggtgttgt cggtcgcgag cgcggagagc
      901 ggctggaact cgtcgccgtt ctggtagtcg acgagtgtcg agtggcgatg gctgaacgtg
      961 ccacgccgcg agtcctgacc cgacaggcgg atcgacgtgc cctcgagcag cgtcgtgccg
     1021 aacgcgaacg cctcggcgag cgcccagtcg acctcgccgt cgtgatacat cttcgtccgc
     1081 gtctcgaact gcttcacgag cttggggtgc accgtgaacc cgtcgggcac ggcgctgagc
     1141 gcggcgtaca cctcgtcgag gcgctccttg gtcacgctgg tgttcgcctt cgggagcaca
     1201 ccggtcgccg gcgggtgcgg cttcgcgatg acctcttcgg tcggtgccga ctggcgcgtt
     1261 tcctcgagcg ccgcctgcag gcgggcgttg aagtcggcga ggaacagctc ggcctcgtcg
     1321 aggctgatgt cgccgcggcg gacgagggcc tcggtgtaca gcttgcgaac gctgcgacga
     1381 gcgtcgatct gccggtacat gatcggctgc gtgtagctcg ggtcgtcgcc ctcgttgtgg
     1441 ccgtggcgcc ggtagcagac catgtcgatg acgacgtcct tgttgaaccg ctggcggtag
     1501 gcgagcgcga gacgcgccac acggacgcac gcctcggggt cgtcgccgtt cacgtggaag
     1561 atcggcgcct gcacggtctt ggcgacgtcg gtgcagtact cggaactccg tgcggcagcg
     1621 ggagcggtcg tgaacccgag ctggttgttg atgatgaggt ggatggtgcc accgacgcgg
     1681 tagcccttga tgagcgacag gttgagcgtc tcggcgacca cgccctgtcc ggcgaacgct
     1741 gcgtcgccgt ggatgagaac cggcagcacc gggaagcgct gcgtggcgcc ctcggggatg
     1801 ttgtccatct tcgcccgcac catgccctcg acgacggggt tgaccgcctc gaggtggctc
     1861 gggttggcgg cgagttcgac ggggatggtg ttgccgctcc gcgccgtgaa cgtgcccgtc
     1921 tggccgaggt ggtacttgac gtcgccggac ccctggatgg aatcggggtc gacgtggccc
     1981 tcgaactcct tgaacagctg gccgtagctc ttgcccacga tgttcacgag cacgttgaga
     2041 cggccgcggt gggccatgcc gagcacggca ccggccatct gctgatcggc cgaccggtcg
     2101 aggatggcgt cgaggatcgc gatggtcgac tcggcccctt cgagaccgaa gcgcttctgg
     2161 cccatccact tggtggcgag gaacttctcg atggcttcgg ccgcgttcat gcgatcgagg
     2221 atgtggcgct gttcggtcgg gtcgagcttg gtcgaggcgc cctcgacctg ctcttggatc
     2281 cagcgcttct cctcgatcga ggagatgtgc gtgtactcga tgcccgacgt gcggcagtac
     2341 gcgtcgcgca acaccccgag gatgtcgccg agcttcatgc gctccttgcc gccgaccggc
     2401 gcatacacgc cggcttcgaa gccggtgagg aactcacggt cgttgtccca gatcgtgagg
     2461 ccgtagaacg ccgggtcgag ctcggggtgc atctggggcg gattcgcggc gagcgggtcg
     2521 aggtcggcga tgaggtggcc gcgcacccgg tacatgttga tgaggttcga gaccttcatc
     2581 tgcttggtga ccgccgattc ctcgcggtcg acggggttgt cgtcggtgtg ccattgcacg
     2641 ggcgtgtagg gcactccgat ggcggcgaag atgtcctcgt agaaccggtc gccaccgagc
     2701 agcatgtcgt ggaccttctt gaggaacagc cccgactcgg caccctggat gatgcggtgg
     2761 tcgtaggtgg agctgatcgt catggtcttg ctgatgccga ggtcggcgat ggtgcgcggg
     2821 tcggccgcgg cgaagcccgt cgggtacgcg agggaaccca cgccgacgat gaggccctgg
     2881 cccggcatga ggcgcggcac cgagcgctcg gtgccgatcg tgcccgggtt ggtgaggctg
     2941 acggtcacgc cggcgaagtc gtcgggcgtg agcttgttgt tgttggcctt gcggatgatc
     3001 tcgtcgtagg tcgcgatgaa gttcttgaag tccatctgat cggcgccctt gatggcggcg
     3061 acgatgaggc tgcgcgagcc gtccttcttc tcctggtcga tggccaagcc gaggttgacg
     3121 ggtcgatcct gcaggatccg gggctcgccg tcgggacctt cgacgtacgc attgttcatg
     3181 cggggcaggt ggtcggcgat ggcgcggacg accgcgtagc cgatgatgtg ggtgaagctc
     3241 accttgccgc cacgcgtgcg gccgaggtag ccgttgatca ccgagcggtt gacctcgagc
     3301 aggcgggccg gcacctcacg gaaactcgtc gcggtcggga ccgagagcga cttctccatg
     3361 ttctcgacga tcttcttgcc gacgccgcga atcgggtcgc ccggcaccgc cggcggcgcg
     3421 gccgggggct ctgtcgccgc gggcgcg
//
LOCUS       DAOSOU010000222         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27610_length_3433_cov_5.953523, whole genome shotgun sequence.
ACCESSION   DAOSOU010000222 DAOSOU010000000
VERSION     DAOSOU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27610_length_3433_cov_5.953523"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLW15_10330"
     CDS             complement(<1..221)
                     /locus_tag="PLW15_10330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT34829.1"
                     /translation="MSHHTAVATITTNLGTIKVNLFGNHAPKTVANFVGLATGTKEWT
                     HPATGKVSTDKLYDGVIFHRIIKQFMLQGG"
     gene            359..907
                     /locus_tag="PLW15_10335"
     CDS             359..907
                     /locus_tag="PLW15_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34830.1"
                     /translation="MALSRKRQKELNRLKGQAEDLWEDQKELLDHAAKVVREASRQAA
                     HYTREEVAPRVRETYEDRVRPAVASGVAGARSAAQTTRDRLVDDVLPAMSSALGTALA
                     AIEVAKNKQVREAISRATKFGTDVGTQVGLIKPKPAGPGRYILIGIGVVAAAAVAYAA
                     WQTLRADDDLWVEDEPSDATAG"
     gene            968..2050
                     /locus_tag="PLW15_10340"
     CDS             968..2050
                     /locus_tag="PLW15_10340"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOT34831.1"
                     /translation="MAEPVLQVRSESRTTVGLESEAVVVGGIAAVLGGAAGAIAFAGS
                     APPIWGGWSIGLLAAIVVVVLGVTASYVGYWRARHLTGQEWRLTLPPWKFILDATNVA
                     AVHALIAGIGTIAGFVLLSLSFRGVTLDGWVAAVSVSAAAGLSAYWIYLSVSKIDTGK
                     LSMLLVLFISITVLTSMATASDPLWWEYHFSLLGTLGDKSASLFNISLIIAGLLVTTF
                     SLYLQRDIRALFDTGVLRYRWSARTISTLFIVMGLMLAGVGAFPLNVNATMHNICAAG
                     MSVAFGLLLLASPLVLRGMPWTFFAVTGGFFAAMVGSVILFAVTGYFNLTFFELVVFI
                     IIFGWIATFIRFLSATVAQAQSETAA"
     gene            2050..>3433
                     /locus_tag="PLW15_10345"
     CDS             2050..>3433
                     /locus_tag="PLW15_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34832.1"
                     /translation="MSGLSRRQFLATAAAAAAAAGIPLETVGAAAAAEVTPAADAPST
                     LLQTMLQGGTVKGEYRALVAGPGESYLPRVDLLGRAPDGARVAARRSLLYLGHLSDMH
                     VIDAQSPGRIEPMIVQDHSAWGSAFHPHDPLSLHVTSSTVQSFSALRYSPLTGAPMGA
                     AIVTGDSADMHSHKELRWYIDMLDGVTVDPAVGIGRQFNGVQAWAEAVWAYRPGNPAG
                     GAFGDYGFPKLPTLLDDSVAHPVESPGLPAPWYAVYGNHDTLLLGTFDLSSQLHALAV
                     GGQKSWSLDATASTILGGYAATTSALGRGTDAAGVALGRPGFHAVPADPDRYLFAQAE
                     FMAEHFRTESAPGPVGHGFTKHNLETGQTWWKADLTPFVRAFGLDTCNQVAGPDGAVP
                     DAQFRWLEEELAQAQKDNRLVLIFSHHNSLTLENRAQRPGDTEKLHGAEEFVDLLLRF
                     PVVVGWMNGHT"
BASE COUNT          496 a         1220 c         1129 g          588 t
ORIGIN      
        1 ccaccctgca gcatgaactg cttgatgatg cggtggaaga tcactccgtc gtagagcttg
       61 tcggtcgaca ccttgcccgt ggccgggtgg gtccactcct tcgtgccggt ggccagtccc
      121 acgaagttcg cgaccgtctt cggcgcgtga ttgccgaaga ggttcacctt gatggttccg
      181 agattggtcg tgatcgtggc gacagccgtg tggtgagaca tgggcacaat tcttgcacag
      241 cgcttcacgc ccccagcccg gaagcaccca gcgccctccc agtcaacccg gtaccgcaac
      301 acgttccgcc gtgacaggat gggcttgttt ctcaaccgat cccactggag gtctgaccat
      361 ggcactctca cgcaagcgcc agaaggaact caatcgtctg aagggtcagg ccgaggacct
      421 ctgggaggac cagaaggaac tcctcgacca cgcggcgaag gtcgtgcggg aggcgagccg
      481 ccaggccgct cactacactc gcgaggaggt cgctccccgg gtgcgcgaga cgtacgagga
      541 tcgcgtgcgg cccgccgtcg catccggtgt cgcgggcgcg cgctctgcgg cgcagaccac
      601 gcgcgaccgt ctcgtcgacg atgtgctgcc ggccatgtca tccgctctcg gtaccgctct
      661 tgcggcgatc gaggtcgcga agaacaagca ggtgcgcgag gcgatcagcc gggccaccaa
      721 gttcggtacg gatgtcggta cccaggtcgg tctgatcaag cccaagcccg cgggccccgg
      781 tcgctacatc ctcatcggaa tcggcgtggt ggccgccgcg gccgtggcct acgccgcgtg
      841 gcagaccctg cgcgccgacg acgacctctg ggttgaggac gagccctctg acgcaaccgc
      901 tggttgagta gccgcgaagc ggcgtatcga aaccacgcca gataggcggc ggccctaggc
      961 ttggcgcatg gctgaacccg tgctccaggt gcgctccgaa tcccgaacga ccgtcggcct
     1021 cgagtccgag gcggtggtgg tgggcggcat cgccgccgtg ctcggtggcg cggcgggggc
     1081 gatcgccttc gctggttccg cgccgccgat ctggggtggc tggtcgatcg gcctcctcgc
     1141 cgcgattgtc gtcgtcgtgc tcggtgtgac cgcgagctac gtggggtact ggcgggcccg
     1201 gcacctgacc ggccaggagt ggagactcac gctgccgccg tggaagttca tcctggatgc
     1261 cacgaacgtg gcggccgtgc atgccctgat cgcggggatc ggcacgattg cgggattcgt
     1321 gctgctgtcg ctcagcttcc ggggcgtcac cctcgacgga tgggtcgcag ccgtgagcgt
     1381 ctcggccgcg gcgggcctca gcgcctactg gatctacctg tcggtctcca agatcgacac
     1441 cggcaagctc tcgatgctgc tcgtgctgtt catctcgatc accgtgctca cgagcatggc
     1501 caccgcgtcc gatcccctgt ggtgggagta tcacttcagc ctgctgggca ccctcggcga
     1561 caagtcggcg agcctgttca acatctccct catcatcgcg ggactgctcg tgacgacctt
     1621 ctcgctctac ctgcagcgtg acatccgggc actgttcgac accggggtgc tgcgctaccg
     1681 ctggagcgcg cgcacgatct cgaccctgtt catcgtgatg ggcctgatgc tcgccggggt
     1741 cggcgccttc cccctcaacg tgaacgcgac aatgcacaac atctgcgcgg cgggcatgtc
     1801 ggtcgcgttc gggctcctcc tcctcgcctc gcccctcgtg ctgcgcggga tgccctggac
     1861 cttcttcgcc gtcaccggcg gcttcttcgc ggcgatggtc gggtcggtga tcctgttcgc
     1921 ggtcaccgga tacttcaacc tcacgttctt cgagctggtc gtgttcatca tcatcttcgg
     1981 atggatcgcc accttcatcc gcttcctgag cgccactgtc gcccaggccc agtcggagac
     2041 cgccgcctga tgtcggggct gtcccggagg cagttcctgg cgaccgccgc cgcagctgcg
     2101 gccgcggccg gcatccccct cgagactgtc ggtgcggcgg cggccgccga ggtcacgccc
     2161 gcggccgatg cgccgagcac cctgctgcag accatgctgc agggcggcac ggtgaaaggt
     2221 gagtaccgcg cgctcgtcgc cggccccggc gagagctacc tgccccgcgt cgacctgctc
     2281 ggccgcgccc ccgatggggc tcgcgtggcc gcgcgccgct cgttgctgta cctcggccac
     2341 ctctccgaca tgcacgtgat cgacgcgcag tcgcccggtc gcatcgagcc gatgatcgtg
     2401 caggaccatt cggcctgggg ttccgccttc cacccgcacg acccgctgtc gctgcacgtg
     2461 acctcctcga ccgtgcagag cttctcggcc ctgcgctaca gcccgttgac cggggccccg
     2521 atgggcgcgg cgatcgtcac cggcgacagc gccgacatgc actcccacaa ggagctgcgc
     2581 tggtacatcg acatgctcga tggcgtcacc gtcgatccgg ccgtcggcat cggccggcag
     2641 ttcaacggtg tacaggcctg ggccgaggcc gtgtgggcct accgtcccgg caacccggcc
     2701 ggcggcgcgt tcggagacta cgggtttccg aagctgccca ctcttctcga cgactcggtg
     2761 gcgcatccgg tcgaatcgcc ggggctgccg gccccctggt acgcggtcta cggcaatcac
     2821 gacaccctgc tgctcggcac cttcgacctc agctcgcagt tgcacgcgct cgccgtcggc
     2881 gggcagaagt cgtggtcact ggatgcgacg gcatcgacca tcctcggcgg ctacgccgcg
     2941 accacgagcg cgctcggccg tggcacggat gccgcgggcg tcgctctcgg gcggccgggt
     3001 ttccacgccg ttcccgccga ccccgaccgc tacctgttcg cccaggccga gttcatggcc
     3061 gagcacttcc gcaccgagag cgcgccgggc ccggtcggac acggattcac caagcacaac
     3121 ctcgagaccg gccagacgtg gtggaaggcc gacctcaccc ccttcgtgcg ggcgttcggc
     3181 ctcgacacct gcaatcaggt cgcgggcccc gacggcgccg tgccggacgc gcagttccgc
     3241 tggttggagg aggagctcgc gcaggcgcag aaggacaacc gcctggtgct gatcttcagc
     3301 caccacaaca gcctcaccct cgagaaccgc gcgcagcgcc ccggcgacac ggagaagctg
     3361 cacggcgccg aggagttcgt cgacctgctg ctgcggttcc cggtggtcgt cggctggatg
     3421 aacgggcaca ccc
//
LOCUS       DAOSOU010000223         3432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_27624_length_3432_cov_6.154279, whole genome shotgun sequence.
ACCESSION   DAOSOU010000223 DAOSOU010000000
VERSION     DAOSOU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27624_length_3432_cov_6.154279"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLW15_10350"
     CDS             complement(<1..182)
                     /locus_tag="PLW15_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34833.1"
                     /translation="MTASERVLTRKTIATYAIGSLGTGGFGTLPGLVLVFYLTDTLGI
                     AALAAGVLVTIAKVWDV"
     gene            221..>1779
                     /locus_tag="PLW15_10355"
     CDS             221..>1779
                     /locus_tag="PLW15_10355"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HOT34834.1"
                     /translation="MSETTISRSLSVEFGPLTSDLTTTTGATATVIAPFTGEVLHELP
                     LSSASDVADAAASARAAAVAWRAAGHAHRRAVLLKAHDLLLERRDDLLDILQTETGKT
                     RGQAFEEVFQGANVARYYAVKARGILARHRRRATIPVIMQTRVSYKPKGLIGVITPWN
                     YPLSLSLMDVVPALAAGNGVVQKADNQGALSILASRRAFIEAGVPAALWAVVAGPGDE
                     VGNAVVDAADYVCFTGSTPTGTKVGERAAKALHGASLELGGKNPLIVLDDVVPAKAAA
                     GAVYGCFSSMGQLCVSIERIYVDRKVADEFTSAFAARVSSLAQGSALDFSTDVGSLTS
                     AAQLERVTAHVDDAVAKGATVLAGGKPRPDLGPYFYEPTVLTGVTSEMACFANETFGP
                     VVAIQVVENEAEAVAAANATEFGLNASIFSGSRRRARALAEQIDAGSVNINEGYRASF
                     GSVDAPMGGMKHSGLGRRNGPEGLLRFVEARTVSESTGILALPRTGAQWQRLAPLMLL
                     VLRVLKGVRRG"
     assembly_gap    1780..1879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1880..2235)
                     /locus_tag="PLW15_10360"
     CDS             complement(<1880..2235)
                     /locus_tag="PLW15_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sec-independent translocase"
                     /protein_id="HOT34835.1"
                     /translation="MSFGLTFDKLLVLGIIAVFLLGPERLPYYASQLARLVRNLRDMA
                     NGAKDRMREEMGPDFDDIDWKKLDPRQYDPRRIIREALIDDEPAPTVKPPIESAYAQR
                     QRRLAEGKAAPFDAEAT"
     gene            2311..2943
                     /locus_tag="PLW15_10365"
     CDS             2311..2943
                     /locus_tag="PLW15_10365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT34836.1"
                     /translation="MSDKQLSWKYAEEFVIERDDIALARQHSLELGVEPITASMGAQL
                     AVLAAATAATSIIEVGTGVGVSGLWLMSGAPDAVLTTIDIELDHQTVARKAFLDAGVP
                     AKQVRLIGGRASEVLPRMNESSYDLVFIDADPASVIEYVEHGLRLVRKGGTVLVAHAL
                     WRGRVADPAQRDDTVASFRLLLSEIAKSAAVLSALSPVGDGLLQLTKVSD"
     gene            complement(2975..3148)
                     /locus_tag="PLW15_10370"
     CDS             complement(2975..3148)
                     /locus_tag="PLW15_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HOT34837.1"
                     /translation="MAAMKPRTGDGPMEAVKEGRLIIVRVPLEGGGRLVVSVNDAEAK
                     ELHDALAGVVATA"
     gene            complement(3242..>3432)
                     /locus_tag="PLW15_10375"
     CDS             complement(3242..>3432)
                     /locus_tag="PLW15_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34838.1"
                     /translation="LAVFFPQSSTFRLLVPMFPALGAFAVPRAIWYRVGIVLLFIAGQ
                     WGWIHIGWWVDGYDWTPP"
BASE COUNT          545 a         1155 c         1108 g          524 t
ORIGIN      
        1 acatcccaca ccttggcgat cgtcacgagc acgccggcgg cgagcgcggc gatgccgagg
       61 gtgtccgtca ggtagaacac caggacaagt ccgggcagcg tgccgaagcc tcccgtgccg
      121 agcgaaccga tggcataggt ggcaatggtc ttacgggtga gcacccgctc cgatgcggtc
      181 atctgccgag tgtaacggca cccgagcgta ggctgtcgtc atgtccgaaa ccacgatctc
      241 ccggtcgctc agcgtcgagt tcggcccact cacgagcgac ctcaccacga cgaccggcgc
      301 caccgccacg gtcatcgcac ccttcaccgg cgaggtgctg cacgagctgc cgctgtcgag
      361 tgccagtgat gtggcggatg ccgcggcatc cgcccgcgcc gccgccgtcg cgtggcgtgc
      421 cgcgggccac gcccaccgcc gcgcggtgct gctgaaggct cacgacctac tgctggagcg
      481 cagggacgac ctgctcgaca tcctgcagac ggagaccggc aagacccgcg gtcaggcctt
      541 cgaggaggtc ttccaggggg cgaacgtcgc tcgctactac gcggtcaagg ctcgcggcat
      601 cctcgcccgg caccgccgcc gcgcgacgat ccccgtcatc atgcagaccc gcgtcagcta
      661 caagcccaag ggcctcatcg gcgtcatcac gccctggaac tacccgctgt cgctctcgct
      721 catggacgtc gtgccggccc tcgccgccgg caacggggtc gtgcagaagg ccgacaacca
      781 gggcgccctg tcgatcctcg cctcgcgccg cgcgttcatc gaagccggcg tgcccgccgc
      841 gctctgggcc gtggttgccg gccccggtga cgaggtcggc aatgcggtcg tggatgccgc
      901 cgactacgtc tgcttcaccg gctccacccc cacgggcacg aaggtcggcg agcgcgcggc
      961 gaaggccctc cacggcgcct cgctggagct gggtggcaag aaccccctca tcgtgctcga
     1021 cgacgtggtg cccgcgaagg ccgcagccgg cgccgtctac ggctgcttct cgtcgatggg
     1081 ccagttgtgc gtatcgatcg agcgcatcta cgtcgaccgg aaggtcgccg acgagttcac
     1141 gtccgccttc gcggcacgcg tctcgagcct ggcccagggc tcggccctcg acttctccac
     1201 ggatgtcggt tccctcacct cggccgccca gctcgagcgt gtcacggctc acgtcgacga
     1261 cgccgtcgcc aagggtgcga cggtgctggc cggaggcaaa ccccgacccg acctcggccc
     1321 gtacttctac gagcccaccg tgctcaccgg cgtcaccagc gagatggcgt gcttcgcgaa
     1381 cgagaccttc ggtccggtcg tcgcgatcca ggtcgtggag aacgaagccg aggcggtggc
     1441 ggcggccaat gccaccgagt tcgggctcaa cgcatccatc ttcagcggct cacgtcgccg
     1501 ggcgcgcgcg ctcgccgagc agatcgacgc gggaagcgtc aacatcaacg agggctaccg
     1561 cgcgagcttc ggcagcgtgg atgctccgat gggaggcatg aagcactccg gcctcgggcg
     1621 ccgcaacggt cccgagggtc tgctgcggtt cgtcgaggcc cgcacggtgt ccgagtcgac
     1681 gggcatcctc gcgctgccgc gtacaggtgc gcagtggcag cgtctcgctc cgctcatgct
     1741 gctcgtgctg cgcgtgctga agggcgtgcg gcggggctan nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnng tggcctcggc gtcgaacggc gccgccttgc cctccgcgag
     1921 ccgacgctgg cgctgggcgt aggccgactc gatcggaggc ttgaccgtgg gcgccggctc
     1981 gtcgtcgatc agggcctcgc ggatgatgcg gcgcgggtcg tactggcgcg ggtcgagctt
     2041 cttccaatcg atgtcgtcga agtccggacc catctcctcg cgcatgcgat ccttggcacc
     2101 gttggccatg tcgcggagat tgcgcacgag gcgcgcgagc tgggaggcgt agtacggcag
     2161 acgttcgggt ccgagcagga agacagcgat gatgccgagc accagcagct tgtcgaaggt
     2221 cagaccgaag gacactcgtt caggatagcc gccgccacct gagtctcacg tctacagtgg
     2281 agagcgaacc gaggagcgct ggtaagtcac gtgtcagaca agcaactgag ctggaagtac
     2341 gcggaggagt tcgtcatcga acgcgatgac atcgccctcg cgcgccagca ctcgctcgag
     2401 ctcggcgtcg aaccgatcac cgcctcgatg ggggcgcagc tcgcggttct tgcggcggcg
     2461 acggcggcca cgagcatcat cgaggtcggc acgggcgtcg gcgtgagtgg gctctggctg
     2521 atgtccggag cgcccgacgc ggtgctcacc acgatcgaca tcgagctcga ccaccagacc
     2581 gtggcacgca aggcgttcct ggatgcgggc gtgcccgcca agcaggtgcg cctgatcggg
     2641 ggtcgggcat ccgaggttct gcctcgcatg aacgagtcga gctacgacct cgtgttcatc
     2701 gatgccgacc ccgcctcggt gatcgagtac gtcgagcacg ggcttcgcct ggtgcgcaag
     2761 ggcggcaccg tgctggtagc ccacgccctg tggcgtggaa gggtcgccga tcctgctcaa
     2821 cgggatgaca cggtcgccag tttccggctg ctgctcagcg agatcgccaa gtccgccgcc
     2881 gttctcagcg cgctgtcccc ggtaggggac ggactgctgc agctgacgaa ggtcagcgac
     2941 tagctcgggc gccgaccagg cgccgattct gcgactacgc ggtcgcgacg actccggcga
     3001 gtgcgtcgtg cagttccttg gcctcggcgt cgttgactga cacgacgaga cggccaccgc
     3061 cctccagcgg tacgcgcacg atgatcaggc ggccttcctt gacagcctcc atgggcccgt
     3121 caccggtcct cggtttcatg gctgccatca gatacccctt tcgcgcgctg tgacgaaaaa
     3181 cacagcgtct ctattatcgt tcattccttc gctaccggaa aaccgcgcga aggttaacag
     3241 atcacggcgg ggtccagtcg tagccgtcga cccaccagcc gatgtgaatc caaccccact
     3301 gcccggcgat gaacagcagt actatcccca cccgatacca gatggctcgc ggcacagcga
     3361 aggcccccag cgccggaaac atcggcacca gcagtcggaa agtgctggac tgcgggaaga
     3421 acaccgcgag ca
//
LOCUS       DAOSOU010000224         3390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_28067_length_3390_cov_5.027286, whole genome shotgun sequence.
ACCESSION   DAOSOU010000224 DAOSOU010000000
VERSION     DAOSOU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28067_length_3390_cov_5.027286"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLW15_10380"
     CDS             <1..1017
                     /locus_tag="PLW15_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOT34839.1"
                     /translation="SLHPHNDRGTAVAAAELGYQAGADRIEGCLFGNGERTGNVDLVA
                     LGINLFTQGIDPQIDFSDLDEVKRTAEYANQLKVHERSPWAGDLVYTAFSGSHQDAIK
                     KGFEAMEADAKAQGVSVDQLLWAVPYLPVDPKDLGRGYEAVVRVNSQSGKGGVAYLLK
                     ADHALDLPRKLQIEFSAVVQAKTDAEGGEVTSDEIWDIFQDEYLPAPAEHPEDKWGRF
                     ELLSTRTASDMTGETTLDVRLRVGEEVQAASGTGNGPIAAFLGIMAGEGIDVKLYDYV
                     EHALSAGGDALAAAYVELNVNGKRLWGVGIDPDISTASLKAVVSAVNRAVRTEAGDRE
                     LVGV"
     gene            1019..1732
                     /locus_tag="PLW15_10385"
     CDS             1019..1732
                     /locus_tag="PLW15_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT34840.1"
                     /translation="MNNLDHLTRVTGAFLRDVSDSDPVAPLADIRWTVADLAAHLGEV
                     HRWAAGNARTGKRGERQNVPELSVPLATWYEASRDILLTTLEELDPAADCYTLSAVDR
                     SVAFWHRRQLFETLVHLWDLRSAVDAAAPPAPEVPPEVHADGVSELFDVFLPRSRDAD
                     RVDLGGGIRLIATDTGHVWSFGSDWRREGDVDVAATVSGPAGDLLLWVWNRARARDGV
                     HREGDADVIRRFEKAHVRP"
     gene            complement(1722..2402)
                     /locus_tag="PLW15_10390"
     CDS             complement(1722..2402)
                     /locus_tag="PLW15_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HOT34841.1"
                     /translation="MTPLFVIPLWADLLAVGIGAAQGALFAAQFRDRRLDLLGVAIIG
                     IATGFGGGILRDVLLSEVPAALSSNWYLIVATGAALLGMLLERLISRMGPLVNALDAL
                     TIGLFGAIGTTKALALGLPVVPAIFVGVLSAVGGSILRDLLLNRPIALMHVGSLYAIA
                     AAIGTTSLAVLLGFGVPVFIAATTCVILTFSVRVLAVLFNWSLPEQRAITTVPRIRRT
                     PRGDSPKA"
     gene            complement(2423..3163)
                     /locus_tag="PLW15_10395"
     CDS             complement(2423..3163)
                     /locus_tag="PLW15_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HOT34842.1"
                     /translation="MTILITGGTGTLGRPTAELLTVAGRDIRVLSRTPGPGRVVGDLS
                     TGAGLTEALTGVDTVLHLATTASGRDVAQTTLLVDACRAAGVTHLVYISIVGVDAVPY
                     PYYRAKLACERVIESSGIPCTILRATQFHSFLRMFANLSRRLPVILNLNVPDQPIAVE
                     EVAQRLVELVDAGPSGRVADIGGPEQLRLRDAIELLQKAEGTHKPVWTLPLVGRTIRT
                     FKEGRHMPGLPGYGRETFAEYVEREVRR"
     gene            complement(3207..>3390)
                     /locus_tag="PLW15_10400"
     CDS             complement(3207..>3390)
                     /locus_tag="PLW15_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34843.1"
                     /translation="QGIETVAVVANGAVAMLGVREGHPVALWTLDIAPDGVRRLMIVM
                     NPHKLERFAASALSHS"
BASE COUNT          578 a         1119 c         1173 g          520 t
ORIGIN      
        1 agcctgcacc cgcacaacga ccgcggaacc gccgtcgccg cagccgaatt gggctatcag
       61 gccggtgccg accggatcga gggctgcctg ttcggcaatg gtgagcgcac cggcaacgtc
      121 gacctggtgg cgctgggcat caacctgttc acccagggca tcgacccgca gatcgacttc
      181 agcgacctcg acgaggtcaa gcgcaccgcc gagtacgcca accagctgaa ggtgcacgag
      241 cgcagcccgt gggccggcga cctcgtctac accgcgttca gcggatcgca ccaggacgcg
      301 atcaagaagg gcttcgaggc gatggaggcc gacgcgaagg cgcagggcgt ctcggtcgac
      361 caacttctct gggccgtgcc gtacctgccg gtcgacccca aggacctggg ccgcggctac
      421 gaggccgtcg tgcgcgtcaa ctcgcagtcc ggcaagggcg gagtcgccta cctgttgaag
      481 gccgaccacg cgctcgacct gccgcgcaag ctgcagatcg agttcagcgc cgtcgtgcag
      541 gccaagaccg acgccgaggg tggcgaggtc acgagcgacg agatctggga catcttccag
      601 gacgagtacc tgccggctcc ggccgagcac cccgaagaca agtgggggcg tttcgagctt
      661 ctctccaccc gtactgcgag cgacatgacg ggcgagacca cgctcgacgt gcgcctgcgc
      721 gtcggcgagg aggtgcaggc ggcgagcggc acgggcaacg gtcctatcgc cgcgttcctc
      781 ggcatcatgg cgggggaggg aatcgacgtg aagctctacg actacgtcga gcacgccctg
      841 agcgccggtg gtgacgcgct ggcggccgct tacgtggagc tcaacgtcaa tggcaagcgg
      901 ctgtggggtg tgggtatcga ccccgacatc tcgacggcgt cgctcaaggc cgtcgtgtct
      961 gcggtcaacc gagctgttcg caccgaggct ggcgaccgcg agctcgtcgg cgtctgacgt
     1021 gaacaacctc gaccacctca cgcgggtgac cggggcattc ctgcgtgatg tcagcgactc
     1081 cgacccggtc gctccgctcg ctgacatccg ctggaccgta gccgatctcg cggcccacct
     1141 cggtgaggtg catcggtggg ccgcgggtaa cgctcgcacg ggaaagcgcg gcgagcggca
     1201 gaacgtgccc gagctgtccg tgcctctggc cacctggtac gaggcgagcc gtgacatcct
     1261 gctgaccact ctcgaggagc tcgaccctgc cgcggactgc tacacgctga gcgcggtcga
     1321 ccggtcggtg gcgttctggc accggcgtca gctgttcgag acactcgttc acctgtggga
     1381 cctgcgcagt gcggtggatg ccgcggcgcc cccagcaccg gaggttcccc ccgaggtgca
     1441 cgccgatggc gtgtcagagc tgttcgacgt gttcctgccg cggtcgaggg acgccgatcg
     1501 cgtcgacctc ggtggtggca tccggttgat cgctactgac accgggcacg tgtggagctt
     1561 cggcagcgac tggcggcgtg agggtgatgt cgacgttgca gccacagtgt cgggcccggc
     1621 tggcgacctt ctgctctggg tgtggaatcg cgcgagagcg cgcgacgggg tgcaccgtga
     1681 gggcgatgct gacgtcatca ggcggtttga gaaggcgcac gtcaggcctt aggggagtcc
     1741 cctcgaggag tgcgacggat gcgcggcacc gtcgtgattg cccgctgttc gggtagcgac
     1801 cagttgaaca gcaccgcgag cacccgcacg ctgaaggtca ggatcacgca cgtcgttgcc
     1861 gcgatgaaca ccggcacgcc gaacccgagg agcacggcga ggctggtggt gccgatcgcg
     1921 gccgcgatgg cgtacagcga tcccacatgc atgagcgcga tgggacggtt gagcagcagg
     1981 tcccgcagga tcgatccgcc gaccgccgac agcaccccca cgaagattgc gggaaccacg
     2041 ggcaacccca gcgcgagggc cttggtcgtg cctatggcac cgaacaggcc gatcgtgagt
     2101 gcgtcgagcg cattcaccag cggacccatt cgcgagatca gtcgttcgag cagcataccg
     2161 agcagagcag ccccggttgc gacgatcagg taccagttgg aactgagcgc cgctggcact
     2221 tcggagagca ggacatcgcg caggatgccc ccgccgaagc cagtcgcaat gccgatgatc
     2281 gcgacgccga gcaggtccag ccggcggtca cggaactggg cggcgaacag ggctccctgc
     2341 gccgcgccga ttcccacggc cagcaggtcg gcccagagcg ggatcacgaa gagcggtgtc
     2401 acgcctcaat cgtgccacgt gctcatcgac gcacctcccg ctcgacgtat tcggcaaacg
     2461 tctcacggcc gtagccgggc agccccggca tgtgcctgcc ctccttgaag gtgcgaatgg
     2521 ttcgtccgac gaggggcagc gtccacaccg gtttgtgggt tccctcggcc ttctggagca
     2581 gttcgatcgc gtcgcgaagt cgcagctgct ccgggccgcc gatgtcggcg accctgcccg
     2641 acgggcccgc atccacgagc tcgaccagtc gctgtgcgac ctcctcgacc gcgatcggct
     2701 ggtcgggcac attcaggttc aggatcacgg gaagtcgacg cgagaggttc gcgaacatgc
     2761 gcaggaagct gtggaactgc gtcgcccgca gaatcgtgca cgggatgccc gaactctcga
     2821 tcacgcgctc gcaggccagc ttggcacggt agtaggggta cggcacggcg tcgactccca
     2881 cgatcgagat gtacacgagg tgggtgactc cggctgcccg gcacgcgtcg acgagaaggg
     2941 tggtctgcgc gacatccctg ccgctggctg tcgtcgcgag gtggagcacg gtgtcgacac
     3001 ccgtcagggc ctcggtcagt cccgcgccgg tcgacaggtc gccgacaacg cggcccggtc
     3061 cgggggtgcg gctgaggact cggatgtcgc gtccggcgac ggtgagaagc tccgcggtcg
     3121 ggcgccccag ggttccggtg ccgccggtga tgaggatggt catggtgtcc tttcggtgac
     3181 gttcaccagc tatgaccgat cagcgcctag gaatgtgaca gggcgctcgc cgcaaaccgt
     3241 tcgagcttgt gcgggttcat cacgatcatc agccgtcgaa cgccatcggg ggcgatatcc
     3301 aacgtccaca gagcgacggg atgcccctcc cgcacaccca gcatcgcgac cgcgccattg
     3361 gcgacaaccg ccacggtctc gatgccctgc
//
LOCUS       DAOSOU010000225         3358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_28412_length_3358_cov_4.646685, whole genome shotgun sequence.
ACCESSION   DAOSOU010000225 DAOSOU010000000
VERSION     DAOSOU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28412_length_3358_cov_4.646685"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLW15_10405"
     CDS             complement(<1..295)
                     /locus_tag="PLW15_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOT34844.1"
                     /translation="MPRDAQGHLLPGVISPRRPVPASIPRPEYVDRREPARYTGTNIY
                     TPDEIERIRNSGRIAAQAVEAVGAAIAPGVTTEQLDAIAHDFLVAHDAYPSTLG"
     gene            330..497
                     /locus_tag="PLW15_10410"
     CDS             330..497
                     /locus_tag="PLW15_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminopeptidase"
                     /protein_id="HOT34845.1"
                     /translation="MTDWWYNHKTGEVEEGAQSLSSDRDGPFATREEAARAPEIIAER
                     ARKWAAEDADD"
     gene            complement(494..1333)
                     /locus_tag="PLW15_10415"
     CDS             complement(494..1333)
                     /locus_tag="PLW15_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT34846.1"
                     /translation="MTRGQVRIGMAGWVYPDWRGGFYPTGLTQKNELAYASSHVTSIE
                     LNGSFYSLQKPSSWKSWRDATPDDFVFSVKAPRFITHIRRLDDVHEPLANFFASGILA
                     LGPKLGAILWQLPPSLEFEPYLLERFLEQLPHSTTDALALAQQRGARMTGKEHLEIDA
                     DRPVRHALEVRHSTFAASDFVELLAKYRIAAVYGDSAGKWPVIDEPTTDFRYARLHAD
                     KALHPDGWYEPSEIDRWAATIDGWLEAGQDAYVYFDNDSKVRAPIDAMSLIARMGSAT
                     RPA"
     gene            complement(1347..2162)
                     /gene="panB"
                     /locus_tag="PLW15_10420"
     CDS             complement(1347..2162)
                     /gene="panB"
                     /locus_tag="PLW15_10420"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT34847.1"
                     /translation="MAEQAVKRVRTRHFQSAKENGIKITGLTSYDQLTAGIFDEAGID
                     FLLVGDSAGNNVYGYDTTLPVSIDDLIPLARAVAGAVQRAFVVADMPFGSYETGPDEA
                     LHTAFRFMKETGSHAVKLEGGVRSAEQIRRIVSAGIPVMAHIGFTPQSEHGLGGHVIQ
                     GRGDQVKQLLADAHAVEDAGAFAVVLEMVPSEAAARVTQELRIPTIGVGAGPHVDGQL
                     LVWTDWAGFTKGRIPKFVKQYANLRKNLTDAVTAYRADVEGGSYPGPEHSYDD"
     gene            2266..>3358
                     /locus_tag="PLW15_10425"
     CDS             2266..>3358
                     /locus_tag="PLW15_10425"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOT34848.1"
                     /translation="MDKQRDFVLRTIEERGVKFIRLWFTDVVGALKMVAITPAEVEGA
                     FAEGLGFDGSAIEGFTRAYEADMLAHPDPATFQILPWRGEVDPTARMFCDITTPDGQP
                     AIADPRYVLKRALAKAADRGFSFYTHPEIEFYLLKSSRLVDGKPEPVDSAGFFDNVPG
                     GTAHDFRRRSVRMLEDLGISVEFSHHESGPGQNEIDLRYADALTTADNIMTFRTVIKE
                     VAIEQGVYATFMPKPLSGHPGSGMHTHLSLFEGDSNAFYDAAGHYQLSKIGRQFVAGL
                     LHHAPEITAVTNQFVNSYKRLWGGDEAPSFVTWGHNNRSALVRVPLYKPNKGQSARVE
                     YRAIDSAANPYLTFSLLLAAGLKGIEEGYE"
BASE COUNT          552 a         1083 c         1146 g          577 t
ORIGIN      
        1 accccagcgt cgacggatac gcgtcgtggg cgaccaggaa atcgtgcgcg atcgcatcca
       61 gctgctcggt ggtcacgccg ggtgcgatgg cggcgccgac ggcctcgacc gcctgcgcgg
      121 cgatgcgtcc cgagttgcgg atgcgctcga tctcgtcggg ggtgtagatg ttggtgccgg
      181 tgtatcgggc cggttcgcgg cgatcgacgt actccgggcg gggaatggag gcgggcacag
      241 ggcgtctcgg tgaaatcact ccgggaagga ggtgtccctg tgcgtcccga ggcatagtgt
      301 tgagtctagt ttcgtgaggg aggcactgga tgaccgactg gtggtacaac cacaagaccg
      361 gcgaggtcga agagggagcc cagtcgctgt cctccgatcg cgacgggccg ttcgccacgc
      421 gcgaagaggc tgcgcgagca cccgagatca tcgcggagcg cgcccgcaag tgggccgcgg
      481 aagacgctga cgactaggcc ggtcgagtag ctgagcccat ccgcgcgatg aggctcatcg
      541 cgtcgatcgg cgcgcgcacc ttgctgtcgt tgtcgaagta gacgtaggca tcctgccccg
      601 cttcgagcca gccgtcgatc gttgccgccc agcggtcgat ctcgctcggt tcgtaccagc
      661 catcggggtg cagggccttg tcggcgtgca ggcgcgcgta gcggaagtcg gtcgtcggct
      721 cgtcgatcac cggccacttg cccgcggagt cgccgtacac ggccgcgata cggtacttcg
      781 cgagtagctc gacgaagtcg ctcgccgcga aagttgagtg gcgcacctcg agggcgtgac
      841 gcacgggacg gtcggcatcg atctcgagat gctccttgcc ggtcatccgg gcgccacgct
      901 gctgcgcaag cgcgagagcg tcggtggtcg agtgcggcag ctgctcgagg aagcgctcca
      961 gcaggtaggg ctcgaactcc aggctcggcg gcagttgcca gaggatcgcc ccgagcttcg
     1021 gaccgagcgc gaggatgccc gacgcgaaga agttcgcgag cggctcgtgc acgtcgtcga
     1081 ggcgccggat gtgcgtgatg aacctcggtg ccttcacgga gaacacgaag tcgtcggggg
     1141 tcgcgtcgcg ccagctcttc caactcgacg gcttctgcaa ggagtagaac gagccgttga
     1201 gctcgatgga tgtcacgtgg ctcgaggcgt acgcgagctc gttcttctgc gtcagccctg
     1261 tggggtagaa gccgccgcgc cagtccgggt agacccaccc ggccatgccg atgcgaacct
     1321 ggccgcgcgt catgtgagtc gtcgcgtcag tcgtcgtagg agtgctcggg gccagggtag
     1381 ctgccgccct cgacgtcagc gcgataggcg gtgacggcat ccgtgaggtt cttgcgcaag
     1441 ttcgcgtact gcttcacgaa cttcgggatg cgacccttcg tgaagcccgc ccagtcggtc
     1501 cacaccaaca gctggccgtc gacgtggggc cctgcgccga caccgatcgt ggggatccgc
     1561 aactcctggg tgacccgtgc cgcggcttcg ctcggcacca tctcgagtac gacggcgaac
     1621 gcgccggcat cctcgacggc gtgggcgtcg gcgagaagct gcttcacctg atcgccgcga
     1681 ccctgaatga cgtgaccgcc gagtccgtgc tcgctctgcg gggtgaagcc gatgtgggcc
     1741 atcaccggga tgcccgcgct cacgatccgg cggatctgct cggcactgcg caccccgccc
     1801 tcgagcttca cggcgtggga accggtctcc ttcatgaagc ggaatgcggt gtgcaacgcc
     1861 tcatccggac cggtctcgta ggagccgaag ggcatgtccg ccacgacgaa tgcgcgctgc
     1921 actgccccgg cgaccgcccg ggccaggggg atcaggtcgt cgatcgacac cggaagggtg
     1981 gtgtcgtagc cgtacacgtt gtttccggcc gagtcgccga cgagcaggaa gtcgatcccg
     2041 gcctcgtcga agattccggc cgtcaactgg tcatagctgg tgagaccggt gatcttgatg
     2101 ccgttctcct tggcactctg gaagtgcctg gtgcgaacgc gcttgactgc ctgctctgcc
     2161 atggatcgag tctagtgacg tgtccgaagg cgcaaaccag tagccttaga ccggtgcccg
     2221 aggcgccctt cggcgtggcg acaggcgtga ttcgtgagga gcttgatgga caagcagcgt
     2281 gacttcgttc ttcggacgat cgaagagcga ggggtcaagt tcatccgcct gtggttcacc
     2341 gatgtggtgg gcgcactgaa gatggtcgcg atcacgccgg ccgaggttga gggcgctttc
     2401 gcggagggtc tcggcttcga tggctcggcc atcgaggggt tcacccgggc ctacgaggcg
     2461 gacatgctcg cccaccccga ccccgcgaca ttccagatcc tcccgtggcg cggtgaggtc
     2521 gatccgaccg cccggatgtt ctgcgacatc accacgcccg acggccagcc cgcgatcgcc
     2581 gatccccgct acgtgctcaa gcgtgcgctc gcgaaggccg cggaccgcgg gttctcgttc
     2641 tacacgcatc ccgagatcga gttctacctg ctgaagtcgt cacggctggt cgacggcaag
     2701 ccggagccgg tggattcggc cggattcttc gacaacgtgc ccggtggtac ggcccacgac
     2761 ttccgtcgcc ggtcggtgcg catgctcgag gatctcggca tctcggtgga gttcagccac
     2821 cacgagtcgg gtccggggca gaacgagatc gacctgcgat acgccgacgc gctcacgacc
     2881 gccgacaaca tcatgacctt ccgcacggtg atcaaggagg tggccatcga gcagggcgta
     2941 tacgcgacgt tcatgccgaa gccgctgtcc gggcatccgg gatcgggcat gcacacccac
     3001 ctgtcgctgt tcgagggcga ctccaacgcg ttctacgacg ccgcaggtca ctaccagctc
     3061 tccaagatcg gccgccagtt cgtcgcgggc ctgctgcacc acgcgcccga gatcacggcg
     3121 gtcaccaacc agttcgtgaa ctcctacaag cgactgtggg gcggcgacga agccccgagc
     3181 ttcgtgacct ggggtcacaa caaccgctcc gccctcgtgc gcgtgccgct gtacaagccc
     3241 aacaagggtc agagcgcacg cgtcgagtat cgagcgatcg actcggccgc caacccctac
     3301 ctgacgttct cgcttctgct cgcggccggt ctcaagggca tcgaggaggg ctacgagc
//
LOCUS       DAOSOU010000226         3344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_28565_length_3344_cov_5.609304, whole genome shotgun sequence.
ACCESSION   DAOSOU010000226 DAOSOU010000000
VERSION     DAOSOU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28565_length_3344_cov_5.609304"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLW15_10430"
     CDS             complement(<1..538)
                     /locus_tag="PLW15_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HOT34849.1"
                     /translation="MATSHKEVWQRATVVEVRPVADAVQRIVLAPEHPVAIEPGAHLD
                     VRLALGTDTVQRSYSIVDADISGSRISLSVFRSLHSRGGAGLMHALVPGDTVSVSQPL
                     QDFPLRFGASSYVLLAGGIGITAIAGMARVLRDRGDDYRLIYVGRTRAAMAYLDELIA
                     LHGDRLDVHVGDENTSLDV"
     gene            complement(541..1632)
                     /locus_tag="PLW15_10435"
     CDS             complement(541..1632)
                     /locus_tag="PLW15_10435"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOT34850.1"
                     /translation="MAGTTTAKIFPYPLNTWYVAGWDHEVNRSILARTVAGKPMALYR
                     TEDGRPVALADACWHRLAPLSLGTLIGREDIQCPYHGLRYNAAGRCVSMPAQETINPS
                     ATVPSFPAVERYRYVWVWPGDPALADPDLIPDMHQMTDPEWAGDGQLIEAGCNYQLVL
                     DNLMDLTHEEFVHTSSIGQAELSEAEFITSHTDTTVTVERWMLGIDPPPFWRKNMRDK
                     FPGFEGKVDRWQIIHYQAPSTICIDVGVAKAGTGAPEGDRSQGVNGYVMNTITPETDR
                     TCHYFWAFMRNYCLDSQLITTQLREGVHRVFGEDEAMLIAQQEAIDANPDYEFYSLNI
                     DAGGMWVRRLLERMLTVEGRLPVTPVAAG"
     gene            1719..2312
                     /locus_tag="PLW15_10440"
     CDS             1719..2312
                     /locus_tag="PLW15_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT34851.1"
                     /translation="MSLRYALLALLTAQPMTGYDLAKAFHSSVGHVWHAPDSQIYPQL
                     RRMETEGLVTGTSVPWGPRGTKTRYDITDTGLTAFREWMTEPLEYAREREPAHLRAAY
                     FEWTEPDVVRKQLEAHIAHFTDQRDQWVAQREAIRSRTHPIVRSRLDRYPPEDWDRIV
                     EYKAFAYEGMIARADAEIAWARRGLALGRELGGSSGG"
     gene            complement(2316..2771)
                     /locus_tag="PLW15_10445"
     CDS             complement(2316..2771)
                     /locus_tag="PLW15_10445"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT34852.1"
                     /translation="MATITPFLWLNHPIDEVIAYYSSVFDGVKVDDEQRMPDGSLLTA
                     TVTIAGQRIGLLSGGPEVPPSDAFSLMVSVETQDEVDSYWNALTADGGQESQCGWLKD
                     KYGIAWQIIPTALGRLIGDPDPARAGRAMRAMFAMRKIVIADLEAAAAG"
     gene            complement(2782..>3344)
                     /locus_tag="PLW15_10450"
     CDS             complement(2782..>3344)
                     /locus_tag="PLW15_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT34853.1"
                     /translation="GAVRLDGIDLRSLHPKDLRRAIVMVTQEAYLFSGTVADNIALGK
                     PDATRTEIIAAAKAVGAHEFIESLPAGYDTDVNKRGGRVSAGQRQLLSFARAFIADPV
                     VLILDEATASLDVPSERLVQEGLQTLLADRTAVIIAHRLSTVAIADRVLVMEHGQIVE
                     DGTPRELIAGTGRFAQLHAAWRDSLV"
BASE COUNT          568 a         1098 c         1108 g          570 t
ORIGIN      
        1 gaacatcgag cgacgtgttc tcgtctccta cgtgcacgtc aagccgatcc ccgtgcagag
       61 cgatcagctc gtcgaggtag gccatcgccg cgcgggtgcg cccgacatag atcagccggt
      121 agtcgtcccc acgatcgcga agcactcggg ccattccagc gattgccgtg attccgatcc
      181 cgcccgcaag gagcacgtag ctcgatgcgc cgaaccgcaa cgggaagtcc tgcaaaggct
      241 ggctgacgga gactgtgtcg cccggcacca acgcgtgcat gagccccgct ccgccacggg
      301 aatgcagcga gcggaagacg ctcagcgaga tgcgggatcc gctgatgtcg gcatccacga
      361 tcgagtacga ccgctgaacc gtgtcggtgc cgagcgcaag ccgcacatcg agatgcgccc
      421 cgggttcgat ggccaccgga tgctcgggcg caagcacgat ccgctgcacc gcatcggcga
      481 ccgggcgcac ctccaccacc gtcgcgcgct gccagacctc cttgtgcgag gtggccacgg
      541 tcagcccgcc gcaaccggcg tgacgggtag gcgcccctca acggtgagca tccgctcgag
      601 cagtcgacgc acccacatgc caccggcatc gatgttgagg ctgtagaact cgtagtcggg
      661 gttcgcgtcg atcgcctcct gctgcgcgat gagcatcgcc tcgtcttcgc cgaagacccg
      721 gtggacgccc tcgcgcaact gcgtggtgat gagctgactg tcgaggcagt agttgcgcat
      781 gaacgcccag aagtagtggc aggtgcgatc ggtttcggga gtgatcgtgt tcatgacgta
      841 gccattgaca ccctgactgc ggtcaccctc gggtgcgccg gtccccgcct tggccacgcc
      901 cacgtcgatg cagatggttg acggtgcctg gtagtggatg atctgccagc gatcgacctt
      961 cccctcgaat ccggggaact tgtcacgcat gttcttgcgc cagaagggtg gggggtcgat
     1021 gcccagcatc caccgctcga cagtcacggt cgtatcggtg tggctggtga tgaactcggc
     1081 ctcgctcaac tcggcctgac cgatgctcga ggtgtgcacg aattcctcgt gagtgaggtc
     1141 catcaggttg tcgagcacga gctggtagtt gcagcctgcc tcgatgagct ggccgtcgcc
     1201 agcccactcc ggatcggtca tctggtgcat gtcggggatg aggtcggggt cggcgagtgc
     1261 cgggtcaccc ggccagaccc agacgtagcg gtatcgctcc acagcgggga acgacggtac
     1321 ggtcgcgctg ggattgatcg tctcctgcgc tggcatggac acacagcgcc cggccgcgtt
     1381 gtaccgcaga ccgtgatacg gacactggat gtcctcccgg ccgatgagag taccgagcga
     1441 tagcggcgcc agccgatgcc agcacgcgtc ggcgagcgcg accggtcgac catcttctgt
     1501 gcgatacaga gccatcggct tgcccgccac ggtgcgggca aggatcgacc ggttgacttc
     1561 gtggtcccac ccggcgacgt accaggtgtt gagcggatac gggaagatct tggcggttgt
     1621 cgtgcctgcc atggggctcc tctcactatc taccttgata gttagttgac acagcgtagc
     1681 gcggcacacg gagtcacaca acgcggcaga atcgacccat gagcttgcgc tacgcgctgc
     1741 tggcgttgct gaccgcccag ccgatgaccg gttacgacct cgcgaaggcg ttccattcgt
     1801 cggttggcca cgtgtggcac gctcccgact cgcagatcta cccgcagctc agacggatgg
     1861 agaccgaggg tctcgtcacg ggtacgtccg tgccctgggg cccccgaggg accaagaccc
     1921 ggtacgacat cacggatacc ggtctgaccg cctttcgcga gtggatgaca gagcctctgg
     1981 aatacgcccg cgagcgggag ccggcccacc tgcgtgcggc ctacttcgag tggaccgagc
     2041 ccgacgtcgt tcgcaagcaa ctcgaggcgc acatcgctca tttcactgac cagcgcgacc
     2101 agtgggtcgc gcagagagaa gcgatccgct cccgcaccca cccgatcgtg cggagtcgcc
     2161 tcgaccgcta cccgcccgag gactgggacc gcatcgtcga gtacaaggca ttcgcctatg
     2221 aggggatgat cgcccgcgcc gacgcggaga tcgcgtgggc tcggcgcggg ctcgcgctcg
     2281 gccgtgaact cggaggctca agcggcggct agcgcctatc ccgcagcggc agcctcgagg
     2341 tcggcgatca cgatcttgcg catggcgaac atcgcccgca tcgctcggcc ggcccgcgca
     2401 gggtcggggt caccgatcag gcggcccagt gcggtgggga tgatctgcca ggcaatgccg
     2461 tacttgtcct tcagccagcc gcactgggac tcctggccgc cgtcggcggt gagcgcgttc
     2521 cagtagctgt cgacctcgtc ctgggtctcc accgacacca tgagcgagaa cgcatcggac
     2581 gggggaacct cgggtccacc gctcagcagg ccgattcgct ggccggcgat cgtgacggtc
     2641 gccgtgagca gcgagccgtc gggcatccgc tgctcgtcgt cgactttcac accgtcgaag
     2701 accgatgagt agtaggcaat cacctcgtca atggggtggt tgagccagag gaagggggtg
     2761 atcgttgcca tgtcgccctt tctacaccag cgaatcccgc cacgccgcat gcagttgcgc
     2821 gaaccgaccc gtgccggcga tgagttcgcg tggcgttccg tcctccacga tctggccgtg
     2881 ctccatcacg agcacccggt cggcgatcgc cacggtcgac aggcggtgcg cgatgatcac
     2941 tgccgtgcga tcggcgagga gggtctgcag accctcctga accaggcgct cgctggggac
     3001 gtcgagggat gccgtcgcct cgtcgaggat cagcaccacc gggtcggcga tgaaggcgcg
     3061 cgcgaacgag agtagctgac gctggcccgc gctgacccgt ccgcctcgct tgttcacatc
     3121 ggtgtcgtac ccggcaggca gtgactcgat gaactcgtga gcacccacgg ccttggcggc
     3181 ggcgatgatc tcggtgcgcg tggcatccgg tttgccgagg gcgatgttgt cggcgaccgt
     3241 gcccgagaac aggtaggcct cctgggtgac catgacaatg gcgcgacgca ggtccttcgg
     3301 gtgcaggctg cgcaggtcga tgccgtcgag gcgcaccgcc ccct
//
LOCUS       DAOSOU010000227         3286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_29228_length_3286_cov_6.400495, whole genome shotgun sequence.
ACCESSION   DAOSOU010000227 DAOSOU010000000
VERSION     DAOSOU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29228_length_3286_cov_6.400495"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..911
                     /locus_tag="PLW15_10455"
     CDS             180..911
                     /locus_tag="PLW15_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34854.1"
                     /translation="MRASRFVVAALAVALALPLTACAGPQGPAGADGAAGPAGATGAP
                     GPTGAPGPAGPQGSSGGGATGATGATGPAGPQGIPGVPGAPGAPGAPGDDGAPGAPGV
                     GDSALFYAVMPPDNSEGVATGADVQFPQDGPTTSPGTLRVNESVFALAEIGVYRITFR
                     VPVIESGQLQIMLDGVAVPYAIAGRATGTSDISLTTLVRTTTTETLLSVRNPSGNPSS
                     LTITNFAGGSQASSATLLIELVKAG"
     gene            complement(929..1543)
                     /locus_tag="PLW15_10460"
     CDS             complement(929..1543)
                     /locus_tag="PLW15_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock and DUF1294 domain-containing
                     protein"
                     /protein_id="HOT34855.1"
                     /translation="MASARVDGILVAWNDERGFGFVRRADRSADLFVHVSSFGAMAYR
                     PEVGDVISYRPGTGKDGRPQALDVHGPGRLRTQPKRASASSVVVVLAFVTVLSIVTMA
                     YSVPIQLVGIYVVTSIVTFAMYGYDKARARSGRWRVPETTLLLLGLIGGWPGAIVGQV
                     VFRHKTTKESFRNAFWVTVVINVLVFGAVVAYVAVPAVRGILNG"
     gene            complement(1568..2011)
                     /locus_tag="PLW15_10465"
     CDS             complement(1568..2011)
                     /locus_tag="PLW15_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT34856.1"
                     /translation="MTTWGELRSDAPEFAACVRAAFEAGTNKTLATLRADGSPRISGS
                     ELAFDDHHVTLGMMPGSRKLADVERDPRVALHSPTLEPPPGSLGAGDAKLAGVLVAIS
                     APEGGVPGAYFELQISEAVVTTVQGDELVVESWHPGRGVEVVRRG"
     gene            complement(2094..2474)
                     /locus_tag="PLW15_10470"
     CDS             complement(2094..2474)
                     /locus_tag="PLW15_10470"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOT34857.1"
                     /translation="MARPQFATVAEFLAAQTPQVRSKVELLRTLVTDAEPGTVEAIKW
                     NSPNFSLAGEDRLTVNVDGKGAVRLILHRGTGVAEHKGATTSFTGDPHALLTWHSDIR
                     ASLLVTDDLNIDAARAIVRAWLEY"
     gene            complement(2477..>3286)
                     /locus_tag="PLW15_10475"
     CDS             complement(2477..>3286)
                     /locus_tag="PLW15_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT34858.1"
                     /translation="RVAVYIDFDNIVISQYDAVHGRGAFIKEKARGAAGKTGDRLQEA
                     TVNIGAVLDFASSFGAVAISRAYADWAAPANTRYQKQLVDRAVDLVQLFNTSGTKNGA
                     DIRLAIDVVEDLFRLRDITHIVIVAGDSDYIPLAQSARRLGRTVIGIGVQGSTSAAFK
                     NACDEFSYYGDLVDDEPAPPATAEPVAPDPKRDAVELMVRALRVRRGDDNVDGWVAAS
                     GLKQQMKRLDAGFDEKKIGYKSFTDFVKAQSAVAELQEDGQARRVRLRDRG"
BASE COUNT          534 a         1164 c         1079 g          509 t
ORIGIN      
        1 tacccgcggt tctggctgca aggtcacgcg gtctggcacg tgctgggcgc ggtcggcgcg
       61 ctcctgctca cggtgcactg gcggagcacg ccccacccca gttcgggcca atcgcgcgaa
      121 tagctcacta atcgcccacc ccgggcctag gctgtggtga cctcggaaag caggacgcca
      181 tgcgcgcatc cagattcgtc gtggccgcac tcgcggtcgc actcgcactg ccactgacag
      241 cgtgcgcagg cccgcaaggc ccggcgggag cggatggcgc agccggtccc gctggtgcta
      301 ccggtgcacc cggccccacg ggagcacccg gcccggctgg gcctcagggt tccagcggtg
      361 gcggcgcgac aggcgcgacc ggcgcgactg gcccggccgg cccgcagggt atccccggtg
      421 tccccggagc acccggagcg cccggagcgc ccggcgatga cggcgcgccc ggtgcccccg
      481 gcgtcggcga ctcggctctc ttctacgccg tcatgccgcc ggacaacagc gaaggcgtcg
      541 cgaccggcgc cgacgtgcag tttccgcagg acggtcccac gaccagcccc ggcacactgc
      601 gagtcaacga gtcagtcttc gcgctcgccg agatcggtgt ctaccgaatc acgttccggg
      661 tgccggtcat cgagtccggc caactgcaga tcatgctcga cggcgtcgcc gttccgtacg
      721 cgatcgcggg gcgcgccacc ggtacaagcg acatctccct caccacactc gtgagaacga
      781 caaccaccga gactctactg tcggtgcgca acccgagcgg caacccgagt agtctcacga
      841 tcacgaattt cgccggcggc tcacaagcct cctcggcgac gctgctcatc gagctcgtca
      901 aggctggcta gccaactgcg cagctggttc aaccgttgag gatgcccctc accgcaggca
      961 cggcgacgta ggccacgacg gctccgaaca cgagcacgtt gatcaccacg gtcacccaga
     1021 acgcgttgcg gaaactctcc ttggtcgtct tgtgcctgaa cacgacctgt ccgactatcg
     1081 cgccgggcca gccgccgatc aggccgagca gtagcagggt cgtttccggc accctccatc
     1141 gtcccgacct cgcacgtgcc ttgtcgtagc cgtacatggc gaaggtgacg atgctcgtga
     1201 caacgtagat ccccacgagc tggatcggta cggagtaggc catggtgacg atgctcagca
     1261 cagtgacaaa agccagcacg accaccacgc tgctcgcact cgcgcgcttc ggttgcgtgc
     1321 gcaaccgtcc gggcccgtgc acgtcgaggg cctgcggccg accgtccttg cctgtgcccg
     1381 gccggtagga aatcacgtct ccgacctccg ggcggtacgc catcgctccg aacgacgaca
     1441 catgcacgaa gaggtccgcg gagcggtcgg cgcgccgaac gaacccgaac ccccgctcgt
     1501 cgttccacgc aacgaggatt ccgtcgacgc gcgcgctggc catgcgccga tgatagcggc
     1561 gccgacacta cccgcgccgc accacctcaa ccccgcgccc cggatgccac gactcgacca
     1621 cgagctcgtc gccctgcacg gtcgtcacca ccgcttcgga gatctgtagc tcgaagtacg
     1681 caccggggac cccgccctcg ggcgcagaaa ttgccaccag caccccggcc agcttcgcgt
     1741 cgccagcacc gagagacccg ggcggcggct cgagcgtggg ggagtgcagg gcaactcgcg
     1801 ggtcgcgctc gacgtcagcg agcttgcggg agcccggcat catgccgagc gtgacgtgat
     1861 ggtcgtcgaa cgcgagttcg ctgccactga tccgcggcga cccgtccgcg cgcagtgtcg
     1921 cgagcgtctt gttggtgccc gcctcgaagg cggcgcggac gcaagcggcg aactcgggcg
     1981 catccgatcg aagctctccc caggttgtca tggggccacc atacgaagca cgggcgacat
     2041 ccagtccgcg ctctactcgc cgaggtgtag gcccaggcgc tcgcccggtt gggctagtac
     2101 tccaaccagg cgcgcacgat cgcgcgtgcg gcatcgatgt tcaggtcatc ggtgacgagc
     2161 aggctcgcac ggatgtcgga gtgccacgtc agcagcgcgt gcggatcacc ggtgaagctc
     2221 gttgtggcac ccttgtgctc cgccacgcca gtgccgcgat gcaggatgag gcgcacggca
     2281 cccttgccgt cgacgttgac cgtcagccgg tcctcgccgg cgagcgagaa gttgggcgag
     2341 ttccacttga tcgcctcgac ggtgccaggt tcggcatccg tgacgagagt acggaggagt
     2401 tcgaccttgg agcgcacctg aggggtctgg gcagccagga actccgcgac cgtcgcgaac
     2461 tggggccggg ccaccgctac ccgcgatccc tcagacgcac ccgccgggcc tgcccgtcct
     2521 cctgcagttc ggcgacggcg gactgggcct tcacgaagtc agtgaacgac ttgtagccga
     2581 tcttcttctc gtcgaagccg gcatccagtc gcttcatctg ctgcttgaga ccggatgccg
     2641 cgacccaccc atccacgttg tcgtcgccgc ggcgcacccg caacgctcgc accatgagct
     2701 cgaccgcgtc gcgcttcgga tcgggcgcga caggctctgc ggttgcaggg ggagcgggct
     2761 cgtcgtcgac gagatcgccg tagtagctga actcgtcgca ggcgttcttg aaggcggcgc
     2821 tggtcgatcc ctgcacgccg atgccgatca cggtgcgtcc cagccggcgg gcgctctgcg
     2881 cgagcggaat gtagtcggag tcgccggcca cgatcacgat gtgggtgatg tcgcgcagcc
     2941 ggaagaggtc ctcgacgacg tcgatggcga gcctgatgtc ggcgccgttc ttcgtgccgg
     3001 aggtgttgaa gagttgcacc agatcgacag cccgatccac caactgcttc tgatagcggg
     3061 tgttcgcggg tgcggcccag tcggcgtacg cgcggctgat ggcgacggcc ccgaaggacg
     3121 acgcgaagtc gagcacggcg ccgatgttga cggtcgcctc ctgcagccgg tcgcccgtct
     3181 tgcccgcggc accgcgcgcc ttctccttga tgaaggcgcc gcggccgtgc accgcgtcgt
     3241 actgcgagat cacgatgttg tcgaagtcga tgtagacggc gacgcg
//
LOCUS       DAOSOU010000228         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_29249_length_3284_cov_5.338805, whole genome shotgun sequence.
ACCESSION   DAOSOU010000228 DAOSOU010000000
VERSION     DAOSOU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29249_length_3284_cov_5.338805"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLW15_10480"
     CDS             <1..647
                     /locus_tag="PLW15_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT34859.1"
                     /translation="DGMAWVHAYLPADAAQRAMLNVTRMAQKLAKAGDETRTIAQLRA
                     DVAADLLAGVLGAEGSVGVTVGVTVPVLTLLGGDEPGILEGHGPIDATTARRLAAHAP
                     SFIRILTHPITGTVRDIDRTRYRPPTDLKRMVGLRDVVCTFPGCGRRARDCDLDHTVA
                     WDDDGKTAETNLASLCRHHHRLKHETNWTVDRSNGVSTWTSPSGAIRTSDPPPF"
     gene            764..2521
                     /locus_tag="PLW15_10485"
     CDS             764..2521
                     /locus_tag="PLW15_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT34860.1"
                     /translation="MSDFRPPSSDMNFILEHVVGYRDVAALPGFEHADLETVSGLLDE
                     AADFMSKVVAPTNRIGDKVGAQHDGKGNVTLPPEFKKAYDTYVESGWNTLQFPEQYGG
                     GGFPSVVGLALQEMLQSSNMAFALCPLLTQGAIDALLHYGSDEQKNAYLPKMVSGEWT
                     GTMNLTEPQAGSDVGALTTKAVPNGDGTYAITGQKIYITWGEHDMAEQIVHLVLARTP
                     GAPAGTRGISCFIVPKFILDEDGNPGERNAVTALSIEHKMGIHGSPTCVLNYEGATGY
                     LIGEENMGMRIMFVMMNVARLSVGMQGVALSERAFQQSLAYAKDRRQGLAIGATGKDS
                     AIIEFPDVRRMILTQKASIAAIRRLMLLNASYMDIASHHPDAAERTRATEIVGLLTPI
                     CKSFGTDLGNELTSIGLQVHGGMGFIEETGAAQHVRDIRIAAIYEGTNGIQAADLVGR
                     KLGVRDGASVMEFIAQIRGILGDLDAAGADFASIRTELARQIDALETATTWMLRTGVA
                     DPNAVLSGSSPYLRMWGLVTSAWLLARSALAAAPLDADTAKEQLVMARFFAEQLLPAC
                     AGLLGAATAGSRDLYALDF"
     gene            2568..3212
                     /locus_tag="PLW15_10490"
     CDS             2568..3212
                     /locus_tag="PLW15_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HOT34861.1"
                     /translation="MPELWETPVTYGAIGATQAADLLRYPPTGYRAIERTARIGHGDA
                     RFEFAWASALSWGIQRGSGFRIRPIEAPATVQAQTYTPVSFESDGTPIRPTSAAEHTF
                     GPDGTPFVRPGDTVELHIPVLGPWGVKAPARVVYVVDEPKRKGFAYGTLPGHPEDGEE
                     AFIVSQRTDESVWLTIRAFSRPSSRSWWAVYPALRVAQWYYTRRYLRALAAPID"
BASE COUNT          534 a         1181 c         1073 g          496 t
ORIGIN      
        1 tcgacggcat ggcgtgggtg cacgcgtacc tgccggcaga tgcggcccag cgcgcgatgc
       61 tcaacgtgac tcgcatggcc cagaagctcg cgaaggcagg cgacgagacc cgcaccatcg
      121 cccagctgcg agcggatgtc gcggccgacc tcctcgcggg cgtgctcggc gccgagggtt
      181 ccgtcggcgt cacggtcgga gtcacggtgc ccgtgctcac gcttctcggc ggagatgagc
      241 cgggcatcct cgagggtcac ggcccgatcg acgcgacgac cgcgcgcaga ctcgcggcgc
      301 acgcgccgag cttcatccgc attctgaccc acccgatcac cggcacggtt cgcgacatcg
      361 atcgcacgcg gtaccgtccg ccgaccgacc tcaagcgcat ggtcgggctg cgggatgtcg
      421 tgtgcacgtt cccggggtgc ggtcgccgcg ctcgggattg cgacctcgac cacacggtcg
      481 cgtgggacga cgacggaaag acggccgaga ccaacctggc gagtctgtgt cgacatcacc
      541 atcggctcaa acacgagacg aactggaccg tcgaccgcag taacggtgtg agcacgtgga
      601 cgagcccgtc gggcgcgatc cgaacgagcg acccgccgcc gttctaggcg ggccagcccg
      661 tcctagcgca cacccacaac aaccagcccg cccccgccgc ataccctgtg gcgagcatcc
      721 aaacgctttc tgaccggctc gaagcccggc gtacagtggc gccatgagtg acttcaggcc
      781 gccgtcgtcc gatatgaact tcatcctcga gcacgtggtg gggtaccgcg atgtcgcggc
      841 cctgccggga ttcgagcacg ccgacctcga gaccgtcagc ggtctgctcg acgaggccgc
      901 cgacttcatg tccaaggtcg tcgcacccac caaccgcatc ggcgacaagg tcggtgccca
      961 gcacgacggc aagggcaatg tcaccctccc gcccgagttc aagaaggcgt acgacaccta
     1021 cgtcgagtcc ggctggaaca ccctgcagtt cccggagcag tacggcggcg gcgggttccc
     1081 gtcggtggtc ggactcgccc tgcaggagat gctgcagtca tccaacatgg ccttcgcgct
     1141 gtgcccgctg ctgactcagg gcgcgatcga cgccctcctg cactacggca gcgacgagca
     1201 gaagaatgcc tacctgccca agatggtctc cggcgagtgg accggcacca tgaacctcac
     1261 cgaaccgcag gccggctccg acgtgggtgc gctcacgacc aaggccgtgc cgaacggcga
     1321 cggcacctac gcgatcaccg gccagaagat ctacatcacc tggggtgagc acgacatggc
     1381 cgagcagatc gtgcacctgg tgctcgctcg cacgcccggt gctccggctg gcacgcgcgg
     1441 catctcctgc ttcatcgtgc ccaagttcat cctcgacgag gacggcaacc cgggcgagcg
     1501 caacgccgtc acggcgctgt cgatcgagca caagatgggc atccacggtt ccccgacctg
     1561 cgttctcaac tacgagggcg ccaccggcta cctcatcggc gaggagaaca tgggcatgcg
     1621 catcatgttc gtcatgatga acgtggcccg cctttccgtc ggcatgcagg gtgttgcgct
     1681 cagcgagcgc gccttccagc agtcgctcgc ctacgcgaag gaccgccgcc agggtctcgc
     1741 gatcggcgcg accggcaagg actcggcgat catcgagttc cccgacgtgc gccgcatgat
     1801 cctcacgcag aaggcgtcga ttgctgcgat ccgccgcctc atgctgctga acgccagcta
     1861 catggacatc gccagccacc acccggatgc cgccgagcgc acccgcgcga ccgagatcgt
     1921 cggcctgctc accccgatct gcaagagctt cggcaccgac ctcggaaacg agctcacgtc
     1981 gatcggtctg caggtgcacg gcggcatggg cttcatcgag gagaccggcg ctgcccagca
     2041 cgtgcgtgat atccgcatcg ccgcgatcta cgagggcacc aacggcattc aggccgccga
     2101 cctcgtgggc cgcaagctcg gcgtgcggga cggggcatcc gtcatggagt tcatcgccca
     2161 gatccgcggg atcctgggcg acctggatgc cgcgggcgcc gacttcgcgt cgatccgcac
     2221 cgagctcgcc cgccagatcg acgcgctcga gaccgcgacg acgtggatgc tgcgcaccgg
     2281 cgtcgccgac cccaacgccg ttctgtcggg ctcgagcccg tacctgcgca tgtggggact
     2341 ggtcaccagt gcctggctgc tggcccgttc ggctctcgca gcggcaccgc tggacgctga
     2401 caccgccaag gaacagctgg tcatggcccg gttcttcgcc gagcagctcc tgcccgcctg
     2461 cgccggactg ctgggtgcgg cgacggccgg gtcgcgcgac ctgtacgccc tcgacttcta
     2521 gaggttcgac ttccacccgc tcaggcgtcg tccgctatct tgggacgatg cctgagctct
     2581 gggagacgcc ggtcacctac ggtgccatcg gtgcgacgca agcggccgac ctgctgcgct
     2641 acccgccgac cggctaccgg gcgatcgagc gcacggctcg catcggtcac ggcgacgccc
     2701 gcttcgagtt cgcctgggcc tcggcattgt cgtggggcat ccagcggggc agcggattcc
     2761 gcatccgccc catcgaggcc ccggccaccg ttcaggcgca gacgtacacg cccgtcagtt
     2821 tcgagtcaga cggcaccccg atccggccga cgagcgcggc cgagcacacc ttcgggccgg
     2881 acgggacgcc ctttgtgcgc cccggcgaca ccgtggagct gcacatcccg gtgctgggtc
     2941 cctggggggt gaaggccccg gcgcgggtgg tctacgtggt cgacgagccg aagcgcaagg
     3001 gtttcgcgta cggcacgctg ccggggcatc cggaggacgg cgaagaggcg ttcatcgtct
     3061 cccagcgcac cgacgagtcg gtgtggctca ccatccgggc gttctcccgg ccgagctcgc
     3121 gctcgtggtg ggcggtgtac ccggcgctca gggtcgccca gtggtactac acccgccgct
     3181 atctgcgagc gctcgccgcc ccgatcgact gatcgatcgc gtcgatcgct gcgtcgagcc
     3241 cggtcgcgaa gtcgtcgatc acgagcacgg cgttcgtcgc gcgc
//
LOCUS       DAOSOU010000229         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_29642_length_3249_cov_5.815592, whole genome shotgun sequence.
ACCESSION   DAOSOU010000229 DAOSOU010000000
VERSION     DAOSOU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29642_length_3249_cov_5.815592"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..750
                     /locus_tag="PLW15_10495"
     CDS             34..750
                     /locus_tag="PLW15_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT34862.1"
                     /translation="MFAHRGLALDAPENTLLAFAKAIAIGAEYIETDVHASHDGFPVV
                     AHDPDLTRVAGRDVKVEQLSMAELRRVDLGDGQGFCSLADALDGFPETRFNIDIKSSA
                     AVEPTVRAILAQRAGDRVLVSSFSERRRAAALRELPGVASSASATRFATALLAGKVGL
                     SPVMRRALRGLVAVQVPERALGLSVTTERMIDRLHSAGVEVHIWTVNDPDRMRELLAL
                     GVDGIVTDRADLALEVVRSL"
     gene            842..1207
                     /locus_tag="PLW15_10500"
     CDS             842..1207
                     /locus_tag="PLW15_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001000 - bacterial-type RNA polymerase
                     core enzyme binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOT34863.1"
                     /translation="MADRSLRGMRLGTQSLQSEVGVEFSPRQKSVYRAADGTEFEVVF
                     AADAEVPQQWESPKTGQEGILVGSDGEPVILDTADVKVPRSHWDMLLERRTRAELEEL
                     LQERLDYLRSRRGQEKIGA"
     gene            complement(1165..1905)
                     /locus_tag="PLW15_10505"
     CDS             complement(1165..1905)
                     /locus_tag="PLW15_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOT34864.1"
                     /translation="MADTLVIIPTYNEIDSLERIVGRLRQSVPEVDLLVVDDASPDGT
                     GDLAERLAAADPHLTVLHRDAKDGLGRAYLAGFDVALSGGYQYVVEIDADGSHDPASL
                     PAMLALARAGNDLVIGSRWVKGGAVANWPWVRQAISRAGNWYARVALRSSIRDITAGF
                     RVYRASALRDLDLSAVSSQGYCFQVELAWQLERTGHRIAEHPITFVERATGRSKMHSG
                     IVLEALVRVTIWGVTYAPIFSWPRRDRR"
     gene            complement(1898..>3249)
                     /gene="lnt"
                     /locus_tag="PLW15_10510"
     CDS             complement(1898..>3249)
                     /gene="lnt"
                     /locus_tag="PLW15_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOT34865.1"
                     /translation="GFVGGVAFYFTHIQWASLFLGPLPMSALAVLESLFFAAGALAIT
                     LAYRWIPRAFPTTLGRVGLLPVAVAGLWTAREAWAAVWPYGGFSWGRMGMSLSDSPFA
                     PLYAWLGISGVSFVIVLIAALVVQVTREVGVDPLVRASLPVALVSLVLIVPAWPVDDA
                     GTLRVAAVQGNGKAGYFDSRQPGDLLQAQLDATAPLFGTDVDVVLWPEGSSDYSPVDD
                     PYTAQVFDYVSEQLNAPFVTWAVTQRGEKTYNTSILWESGKGIVDYYDKRHPVPFGEY
                     IPDRAFWRPFAPDLIDLVGREYTPGTTDMVFDINGTKVGINICFDIVDDQLLTESVEQ
                     GARAIFTSSNNADFGRTDESAQQLAIARIRAIETGRSVVNISTVGLSAVIAPDGSIVD
                     QLPWYTPGTMVEDVALSSTITPAVAAGRELEWFVSGLGLAALVVAGLAARAPGRRRG"
BASE COUNT          564 a         1045 c         1126 g          514 t
ORIGIN      
        1 cccagcggct acctctcccc cgctcgaccg cgagtgttcg cccatcgcgg gctcgccctt
       61 gacgcgcccg agaacaccct gctcgcgttc gcgaaggcga tcgcgatcgg ggccgagtac
      121 atcgagaccg atgttcatgc gagccacgat gggttcccgg ttgtcgccca tgaccccgat
      181 ctgacgcgcg tcgctggccg cgatgtgaag gtcgagcagc tcagcatggc cgagctgcgc
      241 cgggtcgatc tgggcgacgg ccagggattc tgctctctcg ctgatgcgct cgatgggttt
      301 cccgagacgc ggttcaacat cgacatcaag tcgtccgctg cggtggagcc gacggtgcgc
      361 gcgattctcg cgcagcgcgc gggtgatcgc gtgctggtgt ccagtttcag tgagcgcagg
      421 cgggccgcgg cgttgcgtga gctgccgggt gtggcgagtt cggcatccgc aactcgcttc
      481 gcgacggctc tgctggcggg caaggtgggc ctctcccccg tgatgcgtcg agcattgcgt
      541 gggctggtgg cggtgcaggt tcccgagcgc gccctgggtc tctcggtgac gacagagcgg
      601 atgatcgacc ggctgcactc cgccggtgtc gaggtgcaca tctggaccgt caacgacccg
      661 gatcgcatgc gcgaattact cgctctgggc gtcgacggga tcgtcacaga ccgtgcagat
      721 ctcgccctcg aggttgtgcg cagcctgtga actggctggg aacggcgcac gcctgaaagg
      781 ttttgcccag cttcggggtt tataactatg aatcgaccgc gaagtaagag gaggccacac
      841 aatggcagac cgtagtttgc gcggaatgag acttgggacc cagagcttac agagcgaagt
      901 gggcgtggag ttctccccgc gacagaagag tgtgtaccgc gccgccgacg gcacggagtt
      961 cgaagtggtg ttcgccgccg acgctgaggt tccccagcag tgggagtctc cgaagaccgg
     1021 tcaggagggc attctcgtcg gttccgacgg tgagcccgtg atcctcgaca cggccgacgt
     1081 gaaggtgccg cgcagccact gggacatgct gctcgagcgg cgcacgcgcg ccgagctcga
     1141 ggagctgctg caggagcgcc tggattacct gcggtcccgt cgtggccagg agaagatcgg
     1201 cgcgtaggta acgccccaga tcgtcacccg caccagcgcc tccagcacga tcccggagtg
     1261 catcttggaa cgccccgtcg ccctctcaac gaaggtgatg gggtgttctg ctatccggtg
     1321 tccggtgcgc tcgagctgcc acgcgagttc gacctggaag cagtacccct gcgacgacac
     1381 ggccgacagg tcgaggtcgc gcagagcgct cgcccggtac acccgaaaac ctgcggtgat
     1441 gtcgcggatg ctcgatcgaa gcgcaacccg ggcgtaccag ttgccggcgc gcgagatcgc
     1501 ctgccgcacc cacggccagt tggcgacggc gccacccttc acccagcgtg agccgatgac
     1561 gagatcgttg ccggcgcggg cgagcgcgag catggctggc aggctggccg ggtcgtggga
     1621 tccgtccgca tcgatctcga ccacgtactg gtagccgccg gacagggcca cgtcgaagcc
     1681 ggcgaggtag gcccgtccga ggccgtcctt cgcgtcccgg tggagaaccg tgaggtgcgg
     1741 atcagcggcg gcgagccgct cggcgagatc tcccgtgccg tcggggctcg cgtcatccac
     1801 caccagcaga tcgacctcgg gcacggactg gcgcagtcgc ccgacgattc gttcgaggct
     1861 gtcgatctcg ttgtaggtcg ggatgatcac cagcgtgtca gccacgacgc ctcccaggag
     1921 ctcgtgcggc cagtcccgca acgaccagag cggcaaggcc gaggcccgag acgaaccatt
     1981 cgagttctcg tccagcggcg accgcggggg tgatcgtcga ggagagcgcg acatcctcga
     2041 ccatggtgcc cggcgtgtac cagggcagct ggtcgacgat cgagccatcg ggtgcgatca
     2101 cggcgctgag cccgacggtg gagatgttga ccacgcttcg tcccgtctcg atggcgcgga
     2161 ttctcgcgat cgccagctgc tgggcagact cgtcggtgcg cccgaagtcg gcattgttgg
     2221 atgacgtgaa gatcgcgcga gcgccctgct cgacggactc ggtcaggagc tgatcgtcga
     2281 cgatgtcgaa gcagatgttg atgcccacct tcgttccgtt gatgtcgaag accatgtcgg
     2341 tcgtgccggg cgtgtactcg cggccgacca ggtcgatgag gtcgggagcg aacggccgcc
     2401 agaacgcgcg gtcgggaatg tactcaccga agggcacggg atgcctcttg tcgtagtagt
     2461 cgacgatgcc cttgcccgac tcccagagga tcgacgtgtt gtaggtcttc tcgccgcgct
     2521 gggtgaccgc ccaggtcacg aacggcgcat tgagctgctc cgacacgtag tcgaagacct
     2581 gtgcggtgta ggggtcgtcc accggactgt agtcactcga gccctcaggc cagagcacca
     2641 catcgacgtc ggtgccgaac agtggagccg tggcatccag ctgcgcctga agcagatcgc
     2701 cgggctggcg actgtcgaag tagccagcct ttccattgcc ctgcacggcg gcgactcgca
     2761 gggtgccggc gtcgtcgacg ggccacgccg gcacgatcag cacgaggctc acgagcgcca
     2821 cggggaggct agcgcgcacg agagggtcga caccgacctc gcgcgtcacc tgcacgacga
     2881 gcgcggcgat caacacgatg acgaagctca ccccggagat gcccagccac gcgtatagcg
     2941 gcgcgaaggg gctgtcagag agcgacatgc ccatccggcc ccaagagaag cccccgtagg
     3001 gccagactgc cgcccaggcc tcgcgggcgg tccagagccc ggccacggcg accggcagca
     3061 aaccgacccg accgagtgtg gtcgggaatg cgcgcgggat ccaccggtac gcgagggtga
     3121 tcgcgagggc accggccgcg aagaacagag actcgagaac cgccagagcc gacatcggca
     3181 gcgggccgag gaacagcgaa gcccactgga tgtgggtgaa gtagaacgcc actcccccga
     3241 cgaatccga
//
LOCUS       DAOSOU010000230         3244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_29707_length_3244_cov_4.496080, whole genome shotgun sequence.
ACCESSION   DAOSOU010000230 DAOSOU010000000
VERSION     DAOSOU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29707_length_3244_cov_4.496080"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLW15_10515"
     CDS             complement(<1..622)
                     /locus_tag="PLW15_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOT34866.1"
                     /translation="MRHIDPDVLALLALGENAGTESDRGHLAACADCRSELENLAHAA
                     AVGRSTLDAGELLQPDERVWRSIQAEVAAPAPVIPLRPRLARRLLAVAAAVVVLVGGV
                     GVTLWALQPAPVTLLASATLDAFPAWVGSTGSAVVEQTAAGARFVDVTFQAPATEGGF
                     REVWLISSDTSQLVSLGVVRGDTGRFTIPDGLDLTRYDLVDISEEPI"
     gene            complement(603..1160)
                     /locus_tag="PLW15_10520"
     CDS             complement(603..1160)
                     /locus_tag="PLW15_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT34867.1"
                     /translation="MTELDDDRIARDFAAGGDRALEDAYRRWSPLVYTLAVRSLGDIG
                     DAEDVTQKTFVAAWTSRSRFDPARSRLSAWLVGIAKNKIADTHEARARIRRLQEQLAA
                     VSTPSEWMTEPVDLADTLLVADEIAQLEPDAQNVMRLAFYDDLTHNEISTRLGIPLGT
                     VKSHIRRSLQRMRTRLEVTHATHRS"
     gene            complement(1171..1782)
                     /locus_tag="PLW15_10525"
     CDS             complement(1171..1782)
                     /locus_tag="PLW15_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HOT34868.1"
                     /translation="MKRLICAAAAVLLLAGCTTTPAVQPAPQPTSTAPSAAVPDIPRS
                     SALLQAPRQAQPPVTVDIPDAGISVPVVPVGVRPDGLMDIPANVDVAGWYSYGSDPFS
                     DTGTTVIAAHVDSLEYGLGPFADLRNLGSGAQIIVTVAGGSSVTYQVESITSIPKAQL
                     PVTDIFDRAGAQRLALITCGGQFDYDTLTYSDNVVVIAVPVGS"
     gene            complement(1816..2661)
                     /locus_tag="PLW15_10530"
     CDS             complement(1816..2661)
                     /locus_tag="PLW15_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HOT34869.1"
                     /translation="MRYKILSGLAVGLLATVGVAAPAQAISSSSSDVYVVHGIPGLVV
                     DVYVDDALTLEDFTFGATGIAGPLDLAAGDYKIQVFAANADPATDTPAIDVPAAEVPA
                     NKSVSLVAQYDASGTPTLGVYVNDISTIAAGQGRVTVRHAAEAGAVAVVVNGANPFGA
                     DFENGEEFKADLPVGDYTAGVAGPGTTTPIIIPTSGTAADISVAEGVNLIVYAVGTGD
                     DLQLITQSISGLHSNPGGVNTGSAGLAADNSPTAIGLSIAAALALLLVAGFAGARVRS
                     ARETD"
     gene            complement(2770..>3244)
                     /locus_tag="PLW15_10535"
     CDS             complement(2770..>3244)
                     /locus_tag="PLW15_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34870.1"
                     /translation="PPGGTVHSAAEAVAFADEVGFPAQGLTLRAAGDGDLAIVKDIPT
                     LGDLTRHVERALGEGSTVTLIADYRAHRSPLRAHRIRALCERMALRLATPCPRCASPG
                     FGRVAPERGVPCAQCGRATDLIAADIDGCGGCGHQERRPRAVTVADPGYCDHCNP"
BASE COUNT          504 a         1034 c         1200 g          506 t
ORIGIN      
        1 cgatcggctc ctccgagatg tccacgaggt cgtagcgggt caggtcgagg ccgtcgggga
       61 tggtgaaccg gccagtgtcg ccgcgcacga ctccgagact caccagctgc gaggtgtccg
      121 aactgatcag ccagacctcc cggaagcccc cctcggtggc aggcgcctgg aatgtgacat
      181 ccacgaaccg cgcaccggcg gccgtctgct cgacgaccgc ggagcccgtc gacccgaccc
      241 aggcagggaa ggcgtccagt gttgcggagg ccagcagcgt cacgggcgcg ggttgcagcg
      301 cccacagggt cacacccact ccacccacga gtacgaccac ggcggcggca accgcgagaa
      361 gacgacgggc gagccgcggc cgcagaggaa tgaccggtgc gggtgccgcc acctcggcct
      421 ggatcgacct ccacacacgc tcgtcgggct gcagaagttc gcccgcatcg agagtggagc
      481 gcccaacggc ggccgcgtgg gcgaggttct ccaactcgga ccggcagtcg gcgcaggcgg
      541 cgaggtgacc gcgatccgac tcggtgcccg cattctcgcc gagggccagc agggcgagta
      601 cgtcaggatc gatgtgtcgc atgagtcacc tccagtcggg ttcgcatccg ctgcaggctg
      661 cgacggatgt ggctcttgac cgtgcccaag ggtatcccca ggcgggtcga gatctcgttg
      721 tgcgtgaggt cgtcgtagaa cgcgagcctc atgacgttct gggcgtcggg ctcgagttgg
      781 gcgatctcgt cggcgacgag cagagtgtca gcgagatcga ccggctcggt catccactcg
      841 ctcggtgtcg agacggccgc cagctgttcc tgcaggcggc ggatgcgcgc gcgagcttcg
      901 tgcgtgtcgg cgatcttgtt cttggcgatt cccaccagcc aggccgagag ccgggagcgc
      961 gcggggtcga agcgggagcg cgaggtccac gccgcgacga aggtcttctg ggtgacatcc
     1021 tcggcgtcgc cgatgtcgcc gagcgaccgc acggcgaggg tgtagacgag gggcgaccag
     1081 cgacggtagg cgtcctcgag cgcgcggtcg ccacccgccg cgaagtcgcg ggcgattcga
     1141 tcgtcatcga gttcggtcat gatccctgac tcaggatccc acgggaaccg cgatgacgac
     1201 cacgttgtcg ctgtaggtga gagtgtcata gtcgaactga cctccgcagg tgatgagggc
     1261 gaggcgctgg gcgccggctc tgtcgaagat gtctgtgacg ggcagctggg ccttggggat
     1321 gctcgtgatc gattcgacct ggtaggtcac ggatgacccg cccgccaccg tcacgatgat
     1381 ctgagcaccg ctgccgaggt tcctcaggtc ggcgaacggg ccgaggccgt actcgagcga
     1441 gtcgacgtgc gccgcgatca cggtcgtgcc ggtgtcgctg aacgggtcgg atccgtagct
     1501 gtaccagccc gcgacgtcca cgttggcggg gatgtccatg agcccgtcgg gccgaacgcc
     1561 gacgggtacc acgggaaccg agatgcccgc atccggaatg tcgacggtga ccggaggctg
     1621 tgcctgccgg ggcgcctgga gcaacgcact cgaccgcggg atgtcaggca cggcagcgct
     1681 gggggccgtg ctggtcggct gtggtgcggg ctgcacggcc ggagtggtcg tgcagcccgc
     1741 gagaaggagc accgcggcgg cggcgcagat cagtcgcttc atcgtggtgc tccttcctgg
     1801 ggtgctaaac gggcgtcagt cggtttcgcg agcggagcga acgcgtgctc ccgcgaagcc
     1861 ggcgaccagc agcagcgcga gcgccgcggc gatgctcagg ccgatggccg tcgggctgtt
     1921 gtcggcagca aggccggcgc ttcccgtgtt gacgccaccc gggttggagt gcagacccga
     1981 gatcgactgg gtgatgagct gcaggtcgtc gcccgtgccg actgcgtaga cgatgaggtt
     2041 gacgccttcg gccacgctga tgtcggcagc ggtgcccgac gtcggaatga tgatcggcgt
     2101 cgtggttccg ggcccagcga caccagcggt gtagtcgccc accgggaggt cggccttgaa
     2161 ctcctcgcca ttctcgaagt cagcgccgaa cgggttcgcc ccgttgacca cgaccgcgac
     2221 cgcaccggct tctgctgcgt gacgcacggt cacgcggccc tgccctgcgg cgatggtcga
     2281 gatgtcgttc acgtacacgc cgagcgtcgg agtacccgag gcgtcgtact gcgcgaccag
     2341 cgacacgctc ttgttcgcag gaacctccgc cgcaggcacg tcgatggccg gggtgtcggt
     2401 cgcggggtcg gcattggcgg cgaagacctg gatcttgtag tcgccagctg cgaggtcgag
     2461 cggaccggcg atgcccgtag cgccgaaggt gaagtcctcg agcgtgagcg cgtcgtccac
     2521 gtagacgtcg acgacaagtc cggggatgcc gtgcacgacg tagacgtcag agctcgacga
     2581 tgagatcgcc tgggcgggcg cggcgacgcc gaccgtggcg agaagtccga ccgcgaggcc
     2641 ggagagaatc ttgtaccgca tgatgtcctc cttggggcag gcggacttcg cctgtcatgt
     2701 agtacttctc gctgaggggt ggtttcggat gcagctggcc cagaattttc tgaattgtgt
     2761 gtccagagct catggattgc agtgatcgca gtaccccggg tcggcaacgg tgacggcgcg
     2821 agggcgacgt tcctggtgcc cgcagccgcc gcatccgtcg atgtcggcgg cgatcaggtc
     2881 tgtcgcgcgc ccgcactgcg cgcacggcac gccccgctcc ggcgcgacgc ggccgaagcc
     2941 gggggaggcg cagcgcgggc agggcgtggc gagacgcagc gccatccgtt cacagagggc
     3001 acggatgcgg tgcgctcgca gcggggaacg gtgcgcccgg tagtcggcga tcagcgtgac
     3061 cgtgctgccc tcgccgaggg cccgctccac atgccgggtc aggtcgccga gcgtggggat
     3121 gtccttgacg atcgcgaggt cgccgtcgcc ggcggcccga agcgtcagcc cctgggcggg
     3181 gaagccgact tcgtcggcga aggcgactgc ctcggcggcg ctgtggacag tgcctcccgg
     3241 gggc
//
LOCUS       DAOSOU010000231         3206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_30103_length_3206_cov_4.615043, whole genome shotgun sequence.
ACCESSION   DAOSOU010000231 DAOSOU010000000
VERSION     DAOSOU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30103_length_3206_cov_4.615043"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLW15_10540"
     CDS             <1..408
                     /locus_tag="PLW15_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT34871.1"
                     /translation="VFDHLRATLRDLFPSIGDLGVTHTWGGPLGVPRDWHATASYDRA
                     KGMAWAGGYVGDGLSTTNLAGRTLADLITGADTELVRLPWVNHSSPSWEPEPLRFLGA
                     NAGLLAMTVADAEERITKRPSLAAKLMGPLVGH"
     gene            487..1044
                     /locus_tag="PLW15_10545"
     CDS             487..1044
                     /locus_tag="PLW15_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT34872.1"
                     /translation="MTTSTSIHPDYITGTWKLDPSHSEVSFTVKHLMISKVRGVFETF
                     DVTIVTAENPHDSTIEATIDVASVNTGNEGRDNHLRTSDFFLVEEHPTMSFVSTSFDG
                     EPDDFSVTGDLTLRGVTKSVVLKGEFGGIITDGYGNTRAGATATTKINRHDFGVNWNA
                     ALEAGGMTLGDDVTITLDLQVILQP"
     gene            complement(1074..3047)
                     /locus_tag="PLW15_10550"
     CDS             complement(1074..3047)
                     /locus_tag="PLW15_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT34873.1"
                     /translation="MSTTTPARPPMRRGPGGGGPFGGMGMPAEKSMNFGPSAKRLLAR
                     LSPHRAGVIIVIALSVVSVTLSVLGPKLLGEATNIIFSGIISKTLPTGVTQQQVIDQL
                     RASGDNQKADFISGMTLTPGQGIDFSALQMVLGIVLLIYVLASVFSWLSSYLLNGIVQ
                     RTVYRLRADVEAKVNVLPLRYFDGMQRGELLSRVTNDVDNIGQSMQQTLSQVITSLLT
                     VVGVLVMMFIISPVLALIALITIPLTLGITAVIAKRSQKLFVAQWTHTGALNGQIEEA
                     YSGHALVKVFGRHREVESRFAEKNAELYEASFGAQFVSGIIMPAMMFVGNLVYVAIAV
                     VGGLFVSNGTMSLGDVQAFIQYSRQFTQPLSQLGSMANLLQSGVASAERVFELLDAPE
                     QSPDPEGSETQAMGEYGGASGRLAFENVSFRYEPEKPLIDDLSLVAEPGQTIAIVGPT
                     GAGKTTLVNLMMRFYEIDSGRITLDGVDIATMSRDALRSRMGMVLQDTWLFAGTIRAN
                     IAYGRPEATEEEIMDAARATYVDRFVHSLPDGYDTLLDDEGSNVSAGEKQLLTIARAF
                     LARPSVLILDEATSSVDTRTEVLVQQAMKALRQDRTSFVIAHRLSTIRDADLILVMEN
                     GAIVEQGTHDELLAARGAYFELYTSQFAAAITD"
BASE COUNT          573 a         1070 c         1019 g          544 t
ORIGIN      
        1 gtgttcgatc acctgcgggc gacactgcgc gacctcttcc cctcgatcgg cgatctgggc
       61 gtgactcata cctggggtgg ccctctgggc gtgccgaggg actggcacgc gaccgccagc
      121 tacgaccggg caaagggcat ggcctgggcc ggcggctatg tgggcgatgg gttgagcacg
      181 accaatctcg cgggccgcac actggccgac ctgatcacgg gagccgatac ggagctcgtg
      241 cgactgccct gggtgaacca ctcctccccg tcgtgggagc cggagccgct gcggttcctc
      301 ggcgccaacg cgggtctgct cgccatgacg gtagcggatg ccgaggagcg aatcacgaag
      361 cggccgtccc tcgcggcgaa gctcatgggg ccgctcgtcg ggcactgatt gttacggccg
      421 cgtaaattta gttgacgcgt ccactaaatc gggaatgatc cagcgatcga tgcactttca
      481 tgcatcatga ctacatcaac cagcattcac cctgactaca tcaccggcac ctggaagctc
      541 gacccgagcc actccgaggt gtcgttcacg gtgaagcacc tcatgatcag caaggttcgc
      601 ggcgtcttcg agaccttcga tgtcacgatc gtcaccgccg agaacccgca cgacagcacc
      661 atcgaggcca cgatcgacgt ggcatccgtc aacaccggca acgaggggcg cgacaaccac
      721 ctgcgcacca gcgacttctt cctcgtcgag gagcacccga cgatgagctt cgtctccacc
      781 tcgttcgacg gcgagcccga cgacttctcc gtcaccggtg acctcaccct gcggggcgtc
      841 accaagagcg tcgttctgaa gggtgagttc ggcggcatca tcaccgacgg ctacggcaac
      901 acccgcgccg gtgcgacggc gaccacgaag atcaaccgtc acgacttcgg cgtcaactgg
      961 aacgccgcgc tcgaggccgg cggcatgacc ctcggcgacg acgtgaccat cacgctcgac
     1021 ctccaggtga tcctgcagcc gtaggcctgc cgctcgcggc ggcgacacgg aggtcagtcc
     1081 gtgatcgccg ccgcgaactg agaggtgtac agctcgaagt aggcaccgcg agcggcgagc
     1141 aactcgtcgt gggtgccctg ctcgacgatg gcaccgttct ccatcaccag gatcaggtcc
     1201 gcatcacgga tcgtcgagag gcgatgcgcg atcacgaaac tggtgcgatc ctgccgcagc
     1261 gccttcatcg cctgctgcac cagcacctcg gtgcgggtgt ccaccgagct ggtggcctca
     1321 tccaggatga gcacgctcgg ccgcgcgagg aaggcccgcg cgatcgtgag cagttgcttc
     1381 tcgcccgcgc tgacgttcga gccctcgtca tcgagcaggg tgtcgtagcc gtcgggcagg
     1441 gagtgcacga aacggtcgac gtaggttgcg cgcgcggcat ccatgatctc ctcctccgtc
     1501 gcctccggcc gcccgtacgc gatgttcgct cggatcgtgc ccgcgaacag ccaggtgtcc
     1561 tgcagcacca tgcccatgcg cgagcgcagc gcgtcgcgcg acatggtcgc gatgtcgaca
     1621 ccgtcgagcg tgatgcgtcc ggagtcgatc tcgtagaaac gcatcatgag gttgacgagc
     1681 gttgtcttgc ctgcgccggt cggtcccacg atcgcgatcg tctgaccggg ttccgcgacg
     1741 agcgacaggt cgtcgatgag cggcttctcc ggctcgtagc gaaacgagac gttctcgaag
     1801 gcgaggcggc cggacgcacc gccgtactca cccattgcct gtgtctccga gccctcaggg
     1861 tcgggcgact gctcgggcgc atccagcaac tcgaacaccc gctccgccga cgcgactccc
     1921 gactgcagga gattcgccat cgaaccgagc tggctgagcg gctgggtgaa ctgacgcgag
     1981 tactggatga aggcctgcac gtcaccgagg ctcatggtgc cgttggacac gaacagtccg
     2041 ccgaccaccg cgatcgccac gtagaccagg tttcccacga acatcatcgc gggcatgatg
     2101 atgccgctca cgaactgtgc gccgaagctg gcttcgtaga gctcggcgtt cttctcggcg
     2161 aaccggctct cgacctcgcg gtggcgaccg aacaccttca cgagggcgtg acccgagtag
     2221 gcctcctcga tctgtccgtt cagcgcaccg gtgtgggtcc actgggcgac gaagagcttc
     2281 tgcgatcgct tggcgatgac cgccgtgatg ccgagcgtca acgggatggt gatgagggcg
     2341 atcagagcca ggaccggcga gatgatgaac atcatcacga gcacacccac gaccgtcagc
     2401 agcgacgtga tcacctggct cagggtctgc tgcatgctct gcccgatgtt gtcgacgtcg
     2461 ttggtgacgc ggctgagcag ctcgccccgc tgcatcccgt cgaagtagcg caacggcagc
     2521 acgttgacct tggcctcgac gtctgccctg aggcggtaga ccgtgcgctg cacgatgccg
     2581 ttgagcagat agctggagag ccaggagaac accgacgcga gcacgtagat gagcagaacg
     2641 atccccaaca ccatctgcag agccgagaag tcgatgccct gcccgggcgt cagcgtcatg
     2701 ccgctgatga aatcagcctt ctggttgtcg ccggatgccc ggagctggtc tatcacctgc
     2761 tgctgggtca cccccgtcgg aagggtcttc gagatgatgc ccgagaagat gatgttcgtg
     2821 gcctcaccga gcagcttcgg ccccagcaca gacagcgtga cgctgacgac cgagagggcg
     2881 atcacgatga tcactcccgc ccggtgcggg ctgagccgcg cgagcagccg cttcgccgat
     2941 gggccgaagt tcatcgactt ctcggcgggc atccccatgc caccgaaggg accgccaccg
     3001 ccggggccgc gcctcatcgg ggggcgcgcg ggtgtggtgg tgctcatgct gcctcctcgg
     3061 ccttcagctg gctgtcgacg atctcggcgt acgtctcgtt cgacgcgagc agctcgtcat
     3121 gggttccccg gccgacgatc tcaccgtcct cgatcacgag aatctggtcg gcatcgacga
     3181 tcgtcgacac ccgctgggca acgatg
//
LOCUS       DAOSOU010000232         3201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_30163_length_3201_cov_6.271774, whole genome shotgun sequence.
ACCESSION   DAOSOU010000232 DAOSOU010000000
VERSION     DAOSOU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30163_length_3201_cov_6.271774"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLW15_10555"
     CDS             complement(<1..640)
                     /locus_tag="PLW15_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOT34874.1"
                     /translation="MSAPSRRIPVAKLTRVIVDNDYAGDPDGLVALAHQLLTESTEVV
                     AITGTTLTPPHDMPGGVPGSAQSAAREVVQWLTPATEPAVPADTSTPFLELTSIPDAS
                     RVILDEAEKSSDLPLFVTCGGPLTNIAAALREDPTLAERMTLCWIGGGAYPEGGWEYN
                     LALDTPAAQFVFNESSAEIHQFPLTTYRQCAYSIAELEFVLSGGGPFGAWLYE"
     gene            complement(637..>1741)
                     /locus_tag="PLW15_10560"
     CDS             complement(637..>1741)
                     /locus_tag="PLW15_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT34875.1"
                     /translation="VVGEDGTRYLFVSGIRRIELTPDGLATVGELEKVYDGWRYPDDW
                     ITEAYALEGPKLFRRGVWFYLVSAVGGTAGPPTGHMVIVARSRSVHGPWENHPDNPIV
                     RTRDASERWWSRGHATIVAGQGDDWWMLSHGYENGYRTLGRQILLEPIGWTDDGWPIA
                     PGDLSRPMDAPRGAVAQTPPLPIEGTIDPASLGERWTFHAPQPGEANRLSREGSSVVL
                     AGKGRGPADSSPLAMVVGEHAYEIDVELELLDGTDVEGGLLLFFNSRLFCGFGITGER
                     MRSYSGGLETHWREPAPATRRIELRLRNDHHIVTGWYRVAGGEWVRHAIRYETSGYHA
                     NTIGDLLSLRPAVYAAGEGSVRFHRFAYRPVTS"
     assembly_gap    1742..1841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2053..>3201
                     /locus_tag="PLW15_10565"
     CDS             2053..>3201
                     /locus_tag="PLW15_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOT34876.1"
                     /translation="MRIAALRERIEGERRVAITPESARQLVDAGLVVTIESGAGAESG
                     YADAAYQAAGAEVAASVALGTVDVLAHVRPLTVETIGALKAGTITVGLASAASELAAV
                     RALRDARITSFALELVPRISRAQSMDALTSQSLVTGYRSVLEASMRFPRFFPLYMTAA
                     GTIPPAKVLVLGAGVAGLQAIATAKRLGAKVSAYDVRAASADEVASMGGTFITLDLEA
                     ADAAGGYAKELAEDRAARQRELLTPHIHAADIIITTALIPGRPAPQLVTAEMLGGMRA
                     GSIIVDLAAESGGNVEGSIAGEDVLVPVVDGVVTLVGAKNMASAMAFDASRLYAKNVA
                     NLVALMTTEGTLTPDFDDEVVAGACLTHDGAVRHEPTAAALEAPGGTKN"
BASE COUNT          498 a         1047 c         1060 g          496 t
ORIGIN      
        1 tctcgtagag ccatgccccg aacggccctc cgccgctgag tacgaactcc agttccgcga
       61 tggagtaggc gcactggcgg taggtcgtga gagggaactg gtggatctcc gcactcgact
      121 cgttgaacac gaactgcgcc gcaggcgtgt ccagtgcgag gttgtattcc cagccgccct
      181 cgggataggc gccgcctccg atccagcaca gagtcatgcg ctcggccagg gtcgggtctt
      241 cgcgcagcgc ggccgcaatg ttcgtcagcg gcccaccgca ggtaacgaag agcgggaggt
      301 cgctcgactt ctcggcctcg tcgaggatga cccgagatgc atcggggatg ctcgtcagct
      361 caagaaaggg cgtcgaggta tcggcgggaa ccgctggctc tgtcgcgggc gtcagccact
      421 gcacgacctc acgcgccgcg ctctgggcac ttcccggtac cccgcccggc atgtcatgcg
      481 ggggagtgag ggtggttccc gtgatggcga cgacctcggt cgattcggtg agaagttgat
      541 gggccagggc caccaatcca tcgggatcgc cagcgtagtc gttgtcgacg atgacccgag
      601 tgagcttcgc caccgggatg cgtcgtgacg gggcactcat gaggtcaccg gacggtacgc
      661 gaagcggtgg aatcgcacgc tgccttcgcc tgcagcgtag acggccggtc gcagactgag
      721 caggtccccg atcgtgttcg cgtgatagcc ggatgtctcg tagcggatcg cgtgacgcac
      781 ccactcccca ccggcaacgc gataccagcc cgtcacgatg tgatggtcgt tgcgcagccg
      841 aagctcgatg cgccgggtcg cgggggcagg ttcgcgccag tgcgtctcca gtcctccgga
      901 gtagctgcgc atgcgctcac cggtgatgcc gaatccgcag aacagtcggc tgttgaagaa
      961 cagcagcagc ccaccctcga cgtcggtccc gtccagcagt tccagctcga cgtcgatctc
     1021 gtaggcgtgc tcgccgacca ccatcgccag cggcgagctg tcggccggcc cccgaccctt
     1081 gccggcgagg acaacggagg agccctcccg ggaaaggcgg ttcgcttcgc ccggctgggg
     1141 cgcatggaat gtccaacgct cgccgagtga cgccgggtcg atcgtgccct cgatcggcag
     1201 agggggcgtc tgcgcgacgg cgccccgcgg cgcatccatg gggcgcgaga ggtctccggg
     1261 cgcgatgggc cagccatcgt cggtccagcc gatcggctcg agcaggatct gccggccgag
     1321 cgttcgatag ccgttctcgt agccgtggga gagcatccac cagtcgtcgc cctgccccgc
     1381 cacgatcgtg gcgtgccccc gcgaccacca gcgctcagaa gcatcccgtg tgcgcacgat
     1441 cgggttgtcg gggtggttct cccacggtcc gtgcaccgag cgggaacgcg cgacgatcac
     1501 catgtgcccg gtcgggggtc ccgcggtgcc gcccacggca ctcaccaggt agaaccagac
     1561 accgcgtcgg aacagcttgg ggccttcgag cgcgtatgcc tcggtgatcc agtcgtccgg
     1621 gtagcgccac ccgtcataga ccttctcgag ttcgcccaca gtcgcgagcc cgtcgggggt
     1681 cagctcgatg cgacggatgc cgctgacgaa gaggtaccgc gtgccgtctt cgccgacgac
     1741 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccatgtga aggctcacat
     1861 gcgaccggaa ttgggttggg tgacaggtgc tcacgcacgc ggcatccggt gcgatcgggg
     1921 cgcggtgggc attcttcggc tcacgttaag agtgcgtctt ctttcggtct cgagcgccgc
     1981 cgccgggggc tcgcatcgtg acggcctaca gtaacgtcga gcccgtttgc gaaagcaatc
     2041 agaaggagcg ccatgagaat cgcagcgttg cgtgaacgga tcgagggcga gcgccgggtg
     2101 gcgatcaccc cggaatcggc tcgccaattg gtcgatgcag gactcgtcgt cacgatcgag
     2161 agtggcgccg gcgccgaatc gggatacgcc gacgctgcgt atcaggccgc cggtgccgag
     2221 gtcgccgcaa gcgttgcgct cggcactgtc gacgtcctcg cccatgttcg gccgctcact
     2281 gtcgagacga tcggggcgct gaaagcgggc acgatcactg tgggtctcgc gtcggcagca
     2341 tccgagctcg ccgccgttcg cgcgctccgg gatgcccgaa tcacctcctt cgcgctcgag
     2401 ctcgtgccgc gcatctcccg tgcccaatcg atggatgccc tgacgtcgca gtcgctcgtc
     2461 accggctacc gcagcgtgct cgaggcgagc atgcggtttc cccgattctt cccgctctac
     2521 atgaccgcgg ccggcacgat tccgccggcc aaggtgctcg tgctcggcgc tggcgtcgct
     2581 ggcctgcagg cgatcgccac ggccaagcgt ctcggcgcca aggtgtccgc gtacgacgtg
     2641 cgggccgcat ccgccgatga ggtggcctcc atgggcggca cgttcatcac cctcgacctc
     2701 gaagcggcag acgccgcagg cggctacgcg aaggaactcg cggaagaccg ggcggcccgc
     2761 cagcgtgaac tgctgacgcc gcacatccac gccgccgaca tcatcatcac gaccgcgctg
     2821 atcccgggac gaccggcacc gcagctggtc accgccgaga tgctcggcgg gatgcgcgcc
     2881 ggctcgatca tcgtggatct cgccgccgag agcggcggaa acgtcgaggg ctcgatcgct
     2941 ggcgaagacg tgctggtgcc cgtggtcgac ggtgtcgtca cgctggtcgg ggcgaagaac
     3001 atggcctcgg cgatggcctt cgacgcctca cgcctctacg cgaagaacgt ggccaacctg
     3061 gtcgcgctca tgacgaccga aggaacgctc acgcccgact tcgacgacga ggtcgtcgcg
     3121 ggcgcatgcc tgactcacga cggggcggtg cggcacgagc cgacggccgc ggcgctcgag
     3181 gctcccggcg gcaccaagaa c
//
LOCUS       DAOSOU010000233         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_31094_length_3131_cov_5.023407, whole genome shotgun sequence.
ACCESSION   DAOSOU010000233 DAOSOU010000000
VERSION     DAOSOU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31094_length_3131_cov_5.023407"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLW15_10570"
     CDS             <1..595
                     /locus_tag="PLW15_10570"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOT34877.1"
                     /translation="RPNTGHLAVAELERRGLVVGTVTQNVDGLHQAAGSRRVVDLHGS
                     LDRVRCLTCGQYYARDAVAERLAAANPWLETPEAIELSPDGDVQIADVASFVTPECSV
                     CGGILKPDVVFFGEFVPREKFDEASALMHRADALLVAGSSLVVNSGIRLLEQAHRRRL
                     PVVIVNRGPTKGDARAAVKIDAGTSETLVALAERLRT"
     gene            622..1263
                     /locus_tag="PLW15_10575"
     CDS             622..1263
                     /locus_tag="PLW15_10575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT34878.1"
                     /translation="MLLYLVRHGETDWNRARRIQGSTDIPLNDTGRGQAAATGRLLAR
                     RDWDAIYSSPLSRAFETASIIAGEVGLGAPERVDALVERHYGEAEGLADPELARRFPG
                     DTPVPGRESREEVAARAMPALLELAERHPDQAIIVVGHGGFIRAVLGVVDPHGTHGMI
                     TNGSVHSLRHDNGTFSLIAFDDPIEEESLHSATENLEEQNAVEQRDSAGSVVR"
     gene            1260..1724
                     /locus_tag="PLW15_10580"
     CDS             1260..1724
                     /locus_tag="PLW15_10580"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOT34879.1"
                     /translation="MTDARAERYAARVAELGLTIHPREMDDSTHTAEQAAAALGCGVE
                     AIVKSLLFVGASGPVLVLASGPNRVDTALIAAVLGEAVTMADAKTVKTVTGSSIGGVP
                     PVGHPEPIRTIMDETLLALPVVWAAAASQNSVFDIEPQRLADATNATIARVS"
     gene            complement(1705..2790)
                     /locus_tag="PLW15_10585"
     CDS             complement(1705..2790)
                     /locus_tag="PLW15_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT34880.1"
                     /translation="MKIIFDCRYTRLGRHDGISRYGASLVEELSKLHPVTMLISDERQ
                     LAMLPDLPWVMGTDPTSAREPWISRLVNPHAPDVVFTPMQTMGPGGRDYALVTTVHDL
                     IYYTHRTPPRDLNPALRLLWRIYHLWWGFQRAVLNRADAHLVDSETTRELMVKHRMTR
                     NPMHVVLLGTEHPKVAPKRSSAVQRTLVYMGSFMPYKNVDLLVRSMEHLPGYRLQLMS
                     RVAPAEQARLEALAPAGSLEFFNGASDEQYESAMKDATALVTASRDEGFGLPVVEAMA
                     LGTPVLISDIPIFREIGGEAAVFFDPDSVESVVAGVRQLEDAEEWKRRSAASVAWAKR
                     YNWPDAAKQLLAVLTSAAESRASSRGR"
     gene            complement(2808..>3131)
                     /locus_tag="PLW15_10590"
     CDS             complement(2808..>3131)
                     /locus_tag="PLW15_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34881.1"
                     /translation="AVRRFTHDQHHTYFSRYATRDSVVEGFEASISSDVSMFAARISQ
                     PTLLIGADNDPITTVAAQRRLETLFPDARLTILTGVGHLVHYEKPAEAARAIVDFLGA
                     GRVAQ"
BASE COUNT          492 a         1045 c         1096 g          498 t
ORIGIN      
        1 gcgacccaac accggccacc tcgccgtggc cgagctcgaa cgtcgcggcc tcgtcgtggg
       61 cacggtgacg cagaacgtcg acggcctgca ccaggcggcc gggagccggc gggtggtcga
      121 cctgcacggt tccctcgacc gggtgcgttg cctcacctgc gggcagtact acgcacgcga
      181 cgcggtcgcc gaacgcctcg ccgcggcgaa cccgtggctg gagacccccg aggctatcga
      241 gctctccccc gacggtgacg tgcagatcgc ggacgtcgca agcttcgtga cgcccgagtg
      301 cagcgtgtgc gggggcatcc tcaaacccga cgtggtcttc ttcggtgagt tcgtgccacg
      361 ggagaagttc gacgaggcga gcgcgctgat gcacagggcc gacgccctgc tcgtcgccgg
      421 gtcatccctg gtcgtcaatt ccggcatccg cttgcttgag caggcgcacc gccgtcgcct
      481 gcccgtggtg atcgtcaatc gcggcccgac gaagggcgac gcccgcgcgg cggttaagat
      541 cgatgcggga acgtcggaga ccctcgtggc gctcgctgag cgtcttcgca cctaggcggt
      601 cggttccccg aagggaaaca cgtgctgctc tacctcgtac gtcatggcga gaccgattgg
      661 aaccgtgcgc ggcgcattca gggcagcacc gacattccgc tgaacgacac cggtcgcggt
      721 caggcggctg ccaccggtcg attgctggca cggcgcgact gggatgccat ctactcgagc
      781 ccgctgtccc gggcgttcga gacggcgagc atcatcgccg gcgaggtggg gctcggtgcc
      841 cccgagcggg tggacgcact cgtcgagcgc cactacggcg aggccgaggg tctggccgat
      901 cccgagctgg cccgtcgttt tcccggtgac acccctgttc ccgggcggga gtcccgcgag
      961 gaggttgcgg cgcgtgccat gccggcgctc ctggagcttg ccgagcgtca tcccgatcag
     1021 gcgatcatcg ttgtcgggca cggcggtttc atcagggcgg tgctcggtgt cgtcgacccg
     1081 cacggcacgc acggaatgat cacgaacggc tccgtgcaca gcctgcgcca cgacaacgga
     1141 acgttctcgc tcatcgcctt cgacgatccg atcgaggagg agtcgctgca ttccgccacg
     1201 gagaacctcg aagaacagaa cgccgtggag cagcgcgact ccgccgggag cgtcgtgcga
     1261 tgacggatgc ccgtgccgaa cgctacgccg cccgggtcgc cgaactgggc ctcaccatcc
     1321 acccccgcga gatggacgac tccacccaca cggccgagca ggccgcagcg gcgctcggct
     1381 gcggcgtcga ggcgatcgtc aagagtctgc tgttcgttgg cgcgtccggc cccgtactgg
     1441 tgctggcatc gggtccgaat cgggtcgaca cggcgttgat cgccgccgtg ctcggtgagg
     1501 cggtgacgat ggccgacgcg aagactgtga agactgtgac gggttcatcg atcggcgggg
     1561 tgcctcccgt gggccacccc gagccgatcc gcacgatcat ggacgagacc ctgctcgcgc
     1621 tgcccgtggt ctgggccgcc gcggcgagcc agaactcggt gttcgatatc gagcctcagc
     1681 ggctggccga cgcgacgaac gcgactatcg cccgcgtgag ctagctcggc tctcggcggc
     1741 cgaggtgagc acggcgagca actgcttggc cgcatccggc cagttgtagc gcttcgccca
     1801 cgcgacggat gccgccgacc ggcgcttcca ctcctcggca tcctcgagct gccgcacccc
     1861 cgcaacgacg gactcgacgc tgtccgggtc gaagaacacc gcggcctctc ccccgatctc
     1921 acggaagatg gggatgtcac tgatcagcac gggcgtgccg agggccatgg cctcgacgac
     1981 gggcaggccg aatccctcgt cccgcgacgc ggtcacgagc gccgtggcat ccttcatcgc
     2041 cgactcgtac tgttcgtcgc tagcgccgtt gaagaactcc agggagccgg ccggagccag
     2101 cgcctcgagc ctcgcctgct cggcgggcgc cacacggctc atcagctgca ggcggtagcc
     2161 cggcaagtgc tccatcgaac gaaccagaag gtcgacattc ttgtacggca tgaacgagcc
     2221 catgtacacg agggtgcgct gcaccgccga tgaccgcttc ggcgcgacct tgggatgctc
     2281 ggtgccgagc agcacgacgt gcatgggatt gcgcgtcatg cggtgcttca ccatgagctc
     2341 acgcgtcgtc tcggaatcga cgaggtgtgc gtcggcgcgg ttgagcaccg cccgctggaa
     2401 accccaccag aggtggtaga tgcgccacag gaggcgcagg gcggggttga gatcgcgcgg
     2461 cggcgtgcgg tgcgtgtagt agatcaggtc gtgcacggtc gtgaccaggg cgtagtcacg
     2521 gccgcccggg cccatggtct gcatcggggt gaacaccacg tcgggcgcgt gcgggttcac
     2581 cagccgcgag atccagggct cccgcgccga cgtcgggtcg gtgcccatga cccacggcag
     2641 gtcgggaagc attgccagct ggcgctcgtc ggagatgagc atcgtcacgg ggtggagctt
     2701 cgacagctcc tcgaccagac tggcaccgta gcggctgatg ccgtcgtgcc ggccgagtcg
     2761 cgtgtatcgg cagtcgaaga tgatcttcat gggctggtcc ttggcgctca ctgggctacc
     2821 cgacccgcgc cgaggaagtc cacaatcgcg cgcgctgcct cggccggctt ctcgtagtgc
     2881 acgaggtgcc cgacgccggt gaggatcgtg aggcgggcat ccgggaacag ggtctcgaga
     2941 cgacgctggg ctgcgacggt cgtgatcgga tcattgtcgg cgccgatcag aagcgtgggc
     3001 tggctgatgc gtgcggcgaa catgctcacg tcggaactga tggatgcctc gaagccctcc
     3061 acaacgctgt cgcgggtggc gtagcggctg aagtacgtgt ggtgctggtc atgggtgaag
     3121 cgccgcacgg c
//
LOCUS       DAOSOU010000234         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_31844_length_3073_cov_5.169649, whole genome shotgun sequence.
ACCESSION   DAOSOU010000234 DAOSOU010000000
VERSION     DAOSOU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31844_length_3073_cov_5.169649"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLW15_10595"
     CDS             <1..357
                     /locus_tag="PLW15_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOT34882.1"
                     /translation="VHQLVSTVRGVLAGGLTGIDAIAACFPAGSMTGAPKQSATLILD
                     SLEQRARGIYSGAFGYVGLDGAIDLAMVIRSIVVDGAGSTVGTGGGITALSDPGEELA
                     EARLKAAALLRVLGVR"
     gene            402..2966
                     /gene="leuS"
                     /locus_tag="PLW15_10600"
     CDS             402..2966
                     /gene="leuS"
                     /locus_tag="PLW15_10600"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT34883.1"
                     /translation="MTEHDERRDDTDAAPDYDIAAIAAKWQPVWDEMKPFATDDPADK
                     RPRKYILDMFPYPSGELHMGHAEAFAFGDIVARYWRQRGFNVLHPIGWDSFGLPAENA
                     AIERGINPATWTYENIDEQKLNFRRYGVSFDWSRVLHTSDPEYYKWNQWLFLKLYEKG
                     LAYRKESWVNWDPVDQTVLANEQVLPDGTSERSGAVVVKKQLTQWYFKITEYADRLLD
                     DLNQLEGSWPAKVLSMQRNWIGRSTGADVDFRVEGSDRTITVFTTRPDTLYGVTFMVV
                     APDSELAAELAAGSTPEVQAAFADYLVQAQKNTEIERQDATRSKTGIPLERWAINPAS
                     GERIPIWTADYVLADYGHGAVMAVPAHDQRDLDFARAFGLPVRVVVDTTAPVTGALQV
                     ITEEMLAEGPPDPVSTGEALTGDGRMINSGPINGLSKNNAIAKIIEQLEAAGTGRAAK
                     TYRLRDWLISRQRFWGTPIPIIHGADGELVPVPFDQLPVVLPDTEGLDLKPKGKSPLA
                     AATDWMTVADPRDGSPAQRDADTMDTFVDSSWYFLRFLSPNDDLEAFSVSEAEKWAPV
                     DQYVGGVEHAILHLLYARFITKVLFDLGYVSFTEPFSALLNQGMVLSEGKKMSKRSRK
                     GTTFTAEVDKHGADALHLTLAFAGPPEDNINWEDVSPAASAKFLARAFRIASDVTSAP
                     EVEWKTGDVALRRLTHRFLADGPALAESLKFNVLVARIMDLVNATRKVIDSGAGPADA
                     AVREAAEVITIALSLFAPYTAEEMWSRLGYQPTVALAGWRKADPTLLIEDSIVAVVQV
                     DGKVRDRLEVSPKISSDELEKLARASAGVIRAIGDREIVNVIVRAPRIVNIATRAS"
BASE COUNT          512 a         1071 c         1015 g          475 t
ORIGIN      
        1 gtgcaccagc tggtgagcac ggtgcgcggt gtgctcgctg gcggactcac cggcatcgat
       61 gcgatagcgg cctgtttccc cgcagggtcg atgacgggcg cgccgaaaca gtccgcgact
      121 ctgatcctcg actcgctcga gcagagagcg cgcggcatct actcgggggc gttcggctac
      181 gtgggactcg acggggcgat cgacctcgcc atggtcatcc ggtcgatcgt cgtcgacggt
      241 gccggatcga ccgtgggtac cggcgggggc atcacggcgc tgtccgaccc aggggaggag
      301 ctcgcggagg cccggctgaa ggccgcggcc ctgctgcggg tcttgggtgt gcgctagttc
      361 ggtacccttg aagcttgccc ctgcaacgaa gtggattccc tgtgaccgag cacgacgagc
      421 gccgcgacga taccgacgcc gcacccgact acgacatcgc cgcgattgcc gcgaagtggc
      481 agcccgtgtg ggacgagatg aagccgttcg cgaccgacga cccggccgac aagcgcccac
      541 gcaagtacat cctcgacatg ttcccctacc cgtcgggcga gctgcacatg ggccacgccg
      601 aggcgttcgc gttcggtgac atcgtggcgc gctactggcg tcagcgcggg ttcaacgtgc
      661 tgcacccgat cggctgggac tccttcggcc tgcccgccga gaacgcggcc atcgagcgcg
      721 gcatcaaccc cgccacctgg acctacgaga acatcgacga gcagaagctc aacttccgtc
      781 gctacggggt gtcattcgac tggagtcggg tgctgcacac cagcgacccc gagtactaca
      841 agtggaacca gtggctgttc ctcaagctct acgagaaggg tctggcctac cgcaaggaga
      901 gctgggtcaa ctgggacccg gttgaccaga ccgtactcgc caacgagcag gtgttgccgg
      961 atggcacgtc cgagcgctcg ggcgccgtgg tcgtcaagaa gcagctcacc cagtggtact
     1021 tcaagatcac cgagtacgcg gaccgcctgc tcgacgacct gaaccagctc gagggctcgt
     1081 ggccggcgaa ggtgctctcg atgcagcgca actggatcgg gcgctccacc ggtgccgacg
     1141 tcgacttccg cgtagagggc agcgaccgca ccatcacggt gttcaccacg cgccccgaca
     1201 cgctctacgg cgtgacgttc atggtcgtcg cccccgactc cgagctcgcc gccgagctgg
     1261 ctgcgggttc gacacccgag gtgcaggcgg ccttcgccga ctacctcgtg caggcacaga
     1321 agaacaccga gatcgagcgt caggatgcga cgcggtcgaa gacgggcatc ccgctcgagc
     1381 gctgggcgat caacccggcc agtggggagc gcatcccgat ctggacggcg gactacgtcc
     1441 tcgccgacta cggccacggc gcggttatgg ctgttccggc ccatgaccag cgcgacctcg
     1501 acttcgcccg cgccttcggg ctgcccgtgc gggtcgtcgt cgacaccacg gctccggtca
     1561 cgggcgccct gcaggtgatc accgaggaga tgctcgcgga gggtccgccc gaccccgtct
     1621 cgaccggcga ggcgctcacc ggtgatggtc gcatgatcaa ctcgggtccc atcaacgggc
     1681 tcagcaagaa caatgcgatc gcgaagatca tcgagcagct cgaggccgcc ggaaccggtc
     1741 gtgccgcgaa gacctaccgt ctgcgcgact ggctgatcag ccggcagcgc ttctggggca
     1801 cgcccatccc gatcatccac ggtgccgacg gcgagctcgt gcccgtgccc ttcgatcagt
     1861 tgccggtcgt gcttcccgac accgagggac tcgatctcaa gccgaagggc aagtcgcccc
     1921 tcgcagccgc gaccgactgg atgaccgtcg ccgacccgcg cgacggcagt cccgcgcagc
     1981 gtgacgccga caccatggac acgttcgtcg acagctcgtg gtacttcctg cgcttcctct
     2041 cgccgaacga cgacctcgag gcgttctcgg tgtcggaggc cgagaagtgg gcgcccgtcg
     2101 accagtacgt cggcggcgtc gagcacgcga tcctgcacct gctgtacgcc cgcttcatca
     2161 ccaaggtgct cttcgacttg ggctacgtga gtttcaccga gccgttcagc gcgctgctga
     2221 atcagggcat ggtgctcagc gagggcaaga agatgtccaa gcgcagccgc aagggcacca
     2281 cgttcaccgc cgaggtcgac aagcacggcg ccgacgcgct gcacctcacg ctcgcattcg
     2341 ccggcccgcc cgaggacaac atcaactggg aggacgtctc gcccgcggca tccgcgaagt
     2401 tcctcgctcg cgcgttccgc atcgccagcg acgtgacgag cgcccccgag gtcgagtgga
     2461 agaccgggga tgtcgcgctg cgccgtctca cgcaccggtt cctggccgat ggccccgctc
     2521 tcgccgagtc gctcaagttc aacgtgctcg tcgcccgcat catggatctc gtcaacgcca
     2581 ctcgcaaggt gatcgacagc ggggccggac ctgccgacgc cgccgtgcgc gaagccgccg
     2641 aggtgatcac gatcgcgctc agcctgttcg cgccgtacac ggccgaggag atgtggtcgc
     2701 ggctgggtta ccagccgact gtcgccctcg caggctggcg caaggccgac ccgacgctgc
     2761 tgatcgagga ctcgatcgtc gccgtcgtgc aggtggatgg caaggtgcgc gatcgcctcg
     2821 aggtgagccc gaagatctcg agcgacgagc tggagaagct cgcgcgggca tcggcgggcg
     2881 tcatccgggc gatcggtgat cgcgagatcg tcaacgtcat cgtgcgcgcc cctcgcatcg
     2941 tcaacatcgc aacccgcgcc agctagttca ccgcacccgc gggcgcctgc tgcggccccg
     3001 cccggccgcg tagcgtcgcc ggggtggcag ccgactccga accccgtcgc gctcgtgtgc
     3061 gcgtgggcat cgg
//
LOCUS       DAOSOU010000235         3072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_31861_length_3072_cov_7.835930, whole genome shotgun sequence.
ACCESSION   DAOSOU010000235 DAOSOU010000000
VERSION     DAOSOU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31861_length_3072_cov_7.835930"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..811)
                     /gene="dcd"
                     /locus_tag="PLW15_10605"
     CDS             complement(242..811)
                     /gene="dcd"
                     /locus_tag="PLW15_10605"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOT34884.1"
                     /translation="MILSDRTIREAIDSGRIVIDPFDPAKVQPSSVDLTLDHRFLVFR
                     NHTRGVIDVKEDLSDLTELIEITEPGAAFLLHPGEFVLGSTAERIGLPDDLVGRIEGK
                     SSLGRLGLLIHTTAGFVDAGFDGGITLELSNVASLPITLYPGMKIGQISFLQMTTPAD
                     VPYGSGALGSKYSGQVGPTASQYWKNFEQ"
     gene            844..2019
                     /locus_tag="PLW15_10610"
     CDS             844..2019
                     /locus_tag="PLW15_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34885.1"
                     /translation="MATRDVSDAGLTELERWFIDRGIPHFIAEYHSSTRVWTRALRFL
                     VPTYLLASLPLGADTWRDAGIEFAATIVCILVAWVLVNLFRRQPPFSVPTRIGRVELG
                     VYVVGPSLGHLILGQWTTAIAAAAGQLVLLGGAYVVTSYALIPLLTWTLKRSVDSLRV
                     AGKATARALPLLLLVVTFFFMTAEVWQVFARLRGLPYGLTLLLFLVSGAAFAANRARR
                     DIDADVCLDDPAELRELVAGTPAAALAARVPSIVPDCDTTLTRRQRINLLMVIVVNQV
                     LLAIVIATVIGLFFLVFGFLGIGGEVIEAWVLHPAHPLWTVTVSGRQLMLSEELIRVT
                     GFLATFSGFYFGVQSTTDPQLREGLDEFAEDNVRQLFAMRQVYLADLASESRNGPTT"
     gene            complement(1971..2567)
                     /locus_tag="PLW15_10615"
     CDS             complement(1971..2567)
                     /locus_tag="PLW15_10615"
                     /inference="COORDINATES: protein motif:HMM:NF037271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT34886.1"
                     /translation="MTFAAASKALLRLTVLEAANRLATDRPWSAVTMAQIAKEAGVSR
                     QTLYNEFGTRDELAAAYVLWASEQFLDEVDREVAAHEGDLRGALAAAFQLFLELAVEH
                     PLVRALGATTGAEGLHALVATSAGAPVVLAATDRLVAITASAFPYVEPLESRRACEVI
                     VRLAISYLTVPTAGVVDAASTVDQVVGPFLDSLARSAR"
     gene            complement(2564..>3072)
                     /locus_tag="PLW15_10620"
     CDS             complement(2564..>3072)
                     /locus_tag="PLW15_10620"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOT34887.1"
                     /translation="SIHNDILNAWAMSVVLFGGLVAVFGVSVLPWLVLQACIGFLMLE
                     VVNYVEHYGLLRERKENGRYERCRPEHSWNANNVASNLALYQLQRHSDHHANPTRRYQ
                     SLRHFDDVPQLPTGYAGMMVLALVPPLWRRVMDPRVLAFYGGDVQRANLQPRRRERLI
                     ARYGSARS"
BASE COUNT          494 a         1057 c         1024 g          497 t
ORIGIN      
        1 accgcccgat ctaccgccgc cagatgatcg acgtgatccg cgagcagacc acggttccga
       61 acgagccgag cgaaccgacg atcggcgccg ctcacttcaa ctgatcgccg cccggccacg
      121 ggctcgggct cagatacaag tgttggcgtt gcggaagccg tttcgggtca ctgcgaaccg
      181 aacggagggt cctgaccgcc acggagtcca gcgtcgggag ctccggccga gacccctcga
      241 cctactgctc gaagttcttc cagtactggc tcgccgtcgg gccgacctgc ccgctgtact
      301 tcgagcccag ggcacccgac ccgtagggca cgtcggccgg ggtcgtcatc tggaggaacg
      361 agatctggcc gatcttcatg cccgggtaga gcgtgatggg caacgacgcg acgttcgaca
      421 gctcgagggt gatgccacca tcgaacccgg catcgacgaa tcccgccgtc gtgtggatca
      481 ggaggccgag ccgaccgagc gagctcttgc cctcgatacg accgacgaga tcgtcaggga
      541 gcccgatgcg ctcggccgtc gacccgagca cgaactcacc ggggtgcaag aggaacgccg
      601 caccgggttc ggtgatctcg atcagctcgg tgaggtcgct caggtcctcc ttcacgtcga
      661 tcacgccgcg ggtgtggttg cggaacacga ggaaccggtg gtcgagcgtg aggtcgaccg
      721 acgacggctg caccttcgcc gggtcgaacg gatcgatgac gatgcggccg gagtcgatcg
      781 cttcgcggat ggtgcggtcg gacaggatca tcgggggcga accgtatctg gtaggaacga
      841 gccatggcga cccgtgacgt gagtgacgcc ggcctcaccg aactcgagcg ctggttcatc
      901 gaccggggca tcccgcactt catcgccgag taccactcgt ccacccgcgt ttggaccagg
      961 gcgctgcggt tcctcgtgcc cacctacctg ctcgcgtcgc tcccgctcgg ggctgacacc
     1021 tggcgcgatg cgggcatcga gttcgccgcc accatcgtgt gcatcctggt ggcgtgggtg
     1081 ctggtcaacc tgttccgccg gcaaccgccg ttcagcgtgc cgacgcgcat cggtcgcgtc
     1141 gagctggggg tgtacgtggt gggcccctcg ctcggccatc tgatcctcgg ccagtggaca
     1201 acagcgatcg ccgccgccgc aggccagctg gtgctgctcg gcggtgcgta cgtcgtcacc
     1261 agctacgccc tgatccccct gctcacctgg accctcaaac ggtccgtcga ctcgctccgg
     1321 gtcgcgggca aggcgaccgc tcgggcgctg ccgttgctcc tgctcgtggt cacgttcttc
     1381 ttcatgacgg ccgaggtctg gcaggtgttc gctcgcctcc gcggtcttcc ctacggtctg
     1441 acgctgctgc tgttcctcgt gtcgggcgcc gccttcgcag cgaaccgtgc ccgccgcgac
     1501 atcgacgcag acgtctgcct cgacgacccc gccgagctcc gagagctcgt ggccggcacc
     1561 ccggcggcgg cgctcgcggc cagggtcccg tcgatcgtgc ccgactgcga cacaacactg
     1621 acgcgccggc agcgcatcaa cctgctgatg gtgatcgtcg tcaaccaggt gttgctcgcg
     1681 atcgtcatcg ccacggtgat cggactgttc ttcctggtct tcggcttcct cggcatcggg
     1741 ggcgaggtca tcgaggcgtg ggtgttgcac ccggcgcacc cgctgtggac ggtcaccgtg
     1801 tcgggccgac aactcatgct gagcgaggaa ctcatccggg tcaccggctt cctcgccacg
     1861 ttctccggct tctacttcgg ggtgcagtcg accaccgacc ctcagctccg agaaggcctc
     1921 gacgagttcg ccgaggacaa cgttcgccag ctgttcgcga tgcgccaggt ctacctggcc
     1981 gacctcgcga gcgaatcgag gaacggaccc accacctgat cgacggtgct cgcagcgtcg
     2041 accacgccag cggtcggtac cgtgagatag ctgatcgcga ggcgcacgat cacctcgcag
     2101 gcccgccggg actcgagggg ctcgacgtac gggaaggccg atgcggtgat cgccaccaac
     2161 cggtcggtcg cggccaacac caccggcgca cccgccgacg tggccaccaa cgcgtggagg
     2221 ccctcggctc cggtggtcgc gccgagcgcg cgcaccaatg ggtgctcgac ggcgagttcg
     2281 aggaacagct ggaaggccgc agcgagcgcc ccgcggaggt caccttcgtg cgccgcgacc
     2341 tcgcggtcga cctcgtcgag gaactgctcc gacgcccaca gcacgtacgc cgctgccagc
     2401 tcgtcgcgcg tgccgaattc gttgtacagc gtctggcgac tgaccccggc ctccttggcg
     2461 atctgcgcca tcgtgaccgc actccacggc cggtcggtgg ccagacggtt cgccgcctcg
     2521 aggaccgtca agcggagcag cgccttggac gccgcggcaa acgtcacgac cgggcggacc
     2581 cgtagcgagc gatgagtcgc tcacgacgac ggggctggag gttggcgcgc tgcacgtcgc
     2641 caccgtagaa ggcgaggacg cgggggtcca tcacacgacg ccacagcggg ggaacgagcg
     2701 cgagcaccat catccccgcg tagccggtgg ggagctgggg cacgtcgtcg aagtgacgaa
     2761 gcgactgata gcgacgcgtc gggttggcgt gatggtcact gtgccgctgc agctggtaga
     2821 gcgcgaggtt cgaggcgacg ttgttcgcgt tccacgagtg ctccggccgg cagcgctcgt
     2881 agcgaccgtt ctccttgcgt tcccgcaaca ggccgtagtg ctcgacgtag ttgacgactt
     2941 cgagcatcag gaagccgatg cacgcctgga gcacgagcca cgggaggacc gagaccccga
     3001 agaccgcgac caacccaccg aacaggacca cgctcatcgc ccacgcgttg aggatgtcgt
     3061 tgtggatcga ga
//
LOCUS       DAOSOU010000236         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_32176_length_3049_cov_4.372077, whole genome shotgun sequence.
ACCESSION   DAOSOU010000236 DAOSOU010000000
VERSION     DAOSOU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32176_length_3049_cov_4.372077"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLW15_10625"
     CDS             complement(<1..207)
                     /locus_tag="PLW15_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34888.1"
                     /translation="MQGYQFSDATSVADQYDANWLMVKLHARKDSARWTTIEPCILTW
                     ELRTLADWFARVALREPKTVTEVNY"
     gene            complement(374..1819)
                     /locus_tag="PLW15_10630"
     CDS             complement(374..1819)
                     /locus_tag="PLW15_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT34889.1"
                     /translation="MSLGGEHTDLFIGGQWRAASAAGRIDVVNPATEEVWASVPDADA
                     ADVDAAVRAARAALPDWRGIGAAARAEVVLRFADELAARGEGMARVITAENGTPIAET
                     SAAASHSAGILRYFATLADLVDAVDVRPFPNAPGSYTEVSREAVGVCALIAPWNFPLG
                     LVMVKLAPALIAGCTVVIKPAAETPLDLRVLLEAAVAAGIPAGAINVITGGRETGSLL
                     VQHPLVDKVAFTGSTEAGRIIAQQCGSLLRPVTLELGGKSAAILLDDVDLDYFGTMLN
                     RTCLRNTGQTCYNSTRILAPRSRYGEIVELVAGVVASTKQGDPLELDTVYGPTVSARQ
                     RDRVEGYIALGRAEGATVATGGGRPAFDRGFFVQPTVFSDVDNRMRIAQEEIFGPVLV
                     VIPFDDDSDAARIANDSQFGLGGSIFSADESRAAALARTIETGSIGVNFFGSNHAAPF
                     GGWKDSGLGVEYGPEGLNAYLRPKSVHRRAG"
     gene            complement(1830..2480)
                     /locus_tag="PLW15_10635"
     CDS             complement(1830..2480)
                     /locus_tag="PLW15_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT34890.1"
                     /translation="MPSQSPSPTIRTLASGFSPDGDWPSLDDVWRAAEPALRARSNDI
                     HTPLSFAFAELLLEAHPEADPLVVRVAILLHDSGWSRVDESKIISEGFRGDWRTSDVR
                     YLHELEGCNIAREILPGLGYEEAFITRVTDIIDGHDTRLQHHSIEDALVRDADRLWRF
                     TPTGIGLASGWFGKTPSQYHEQLKTGTFRELITDSAREIAAAELARSVELLKLDVI"
     gene            complement(2493..>3049)
                     /locus_tag="PLW15_10640"
     CDS             complement(2493..>3049)
                     /locus_tag="PLW15_10640"
                     /inference="COORDINATES: protein motif:HMM:NF026109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HOT34891.1"
                     /translation="LIPSDLVLIGIGVVPRSGLAEQLGLELSGGAIVVDEFARTSDPR
                     VVAAGDAVLLPHPLGGEGRVRLESVQNAVDQAKVAAATLIGKLEPYRAVPWFWSDQAD
                     LKLQIAGLSTGFDEAVLRGNPDDEHFSVLYYRDGHLIAIDSVNDAPDYMTVRRALGQN
                     ATIPADLAADTSTPLKSLVVPSPA"
BASE COUNT          532 a          990 c         1041 g          486 t
ORIGIN      
        1 gtagttcact tccgtcactg tcttcggttc tcgaagggca actcgcgcaa accagtcggc
       61 gagagtccgg agttcccagg tgaggataca tggctcgatg gtcgtccatc gcgctgaatc
      121 cttgcgggcg tgaagcttca ccatcagcca gttcgcatcg tactgatcgg caacggaggt
      181 ggcatccgag aactgatagc cctgcacgcc gagggcgaag ctgagcgatc ccgtgcttcc
      241 ttctactcgc attctgcccc ttcggttcca cgaaaccgtg gagggtcagc atagtagcga
      301 gggtcgcgac agacggcgta tgccaaagat ggtttcgata cgccgcggtt tcgatacgct
      361 gctgcgcggc tactcaacca gccctgcggt gcaccgactt cgggcgcagg tacgcgttca
      421 ggccctcggg gccgtactcg acaccgaggc ccgagtcctt ccagccaccg aacggggcgg
      481 cgtggttgga gccgaagaag ttgacgccga tgctcccagt ctcgatcgtg cgcgcgaggg
      541 cagcagcgcg cgattcgtcg gcggagaaga tcgagccgcc gagaccgaac tggctgtcgt
      601 tggcgatgcg ggcggcatcc gagtcatcgt cgaacgggat gaccacgagc accggaccga
      661 agatctcctc ctgggcgatg cgcatgcggt tgtcgacgtc gctgaacacc gtcggctgca
      721 cgaagaagcc gcggtcgaac gccggccgcc cgccaccggt agcaacggtg gcgccctccg
      781 cgcggccgag cgcgatgtag ccctcgaccc tgtcgcgctg ccgcgccgag acagtcggcc
      841 cgtagaccgt gtcgagctcg agcgggtcac cctgtttggt cgacgccacg acgccagcca
      901 cgagctcaac tatctcgccg taccgcgagc gcggcgcgag gatgcgcgtc gagttgtaac
      961 aggtctggcc cgtgttgcgc aggcaggtgc ggttgagcat cgtgccgaag tagtcgagat
     1021 cgacgtcatc cagcaggatc gccgccgact tgccgcccag ctcgagggtc accggccgca
     1081 gcagcgagcc gcactgctgc gcgatgatgc gccctgcctc ggtcgaaccg gtgaacgcga
     1141 ccttgtcgac caggggatgc tgcacgagca ggctccccgt ctcgcgaccg cccgtgatca
     1201 cgttgatggc tcccgccggg atgcccgcgg cgaccgccgc ctccagcagc accctgaggt
     1261 cgagcggcgt ctcggccgcg ggcttgatca cgacggtgca tccggcgatc agggccggcg
     1321 cgagcttgac catgacgagg ccgagcggga agttccacgg cgcgatgagc gcgcacacgc
     1381 cgaccgcctc gcggctgacc tccgtgtacg agccgggggc gttcgggaac ggccgcacgt
     1441 cgacggcgtc gacgaggtcg gcgagtgtcg cgaagtacct gagaatgccc gccgagtggg
     1501 atgccgcagc cgacgtctcg gcgatgggcg tgccattctc cgcggtgatg acccgtgcca
     1561 tcccctcgcc ccgcgccgcg agctcgtcag cgaagcgcag caccacctca gcgcgcgccg
     1621 cggcaccgat gccccgccag tcgggcagtg cggcgcgggc ggcacgcacg gcggcatcca
     1681 catcggctgc atcagcgtcg ggcactgacg cccagacctc ctcggtggcg ggattcacga
     1741 cgtcgatgcg gccggccgcc gaggccgcgc gccactgacc accgatgaac aggtcggtgt
     1801 gctcgcctcc caggctcacg gaggtcacct cagatcacat cgagcttgag cagctcaacc
     1861 gaccgtgcga gttctgcggc cgcaatctcc cgtgccgaat ccgtgatcag ctcacggaag
     1921 gtgcccgtct tcagctgctc gtgatactgg ctcggggtct ttccgaacca gccggaggcg
     1981 agtccgatgc cggtcggtgt gaaccgccac agtcggtcgg cgtcgcgtac cagggcatcc
     2041 tcgatggagt ggtgctgcag cctcgtgtcg tgaccgtcga tgatgtctgt aacgcgtgtg
     2101 atgaacgctt cctcgtagcc gagacccggc aggatctcgc gggcgatgtt gcacccctca
     2161 agctcatgaa ggtaccgcac atcactcgtg cgccagtcgc cacggaagcc ttcggagatg
     2221 atcttcgatt catccacccg cgaccagccc gaatcgtgca ggaggatcgc aacccggacc
     2281 acgagcgggt cagcctccgg gtgggcttcc agcagcagtt cggcgaacgc gaacgacagc
     2341 ggggtgtgga tgtcgttgga gcgggcacga agggcgggct cggcggcacg ccagacatcg
     2401 tcgagcgagg gccagtcacc gtcgggggaa aaccccgaag cgagcgttcg gatggtcggt
     2461 gagggggact gcgatggcat gggggacctt ttctacgccg ggctggggac gacgagggac
     2521 ttgaggggag tgctggtgtc ggcggcgaga tcagcaggga tggtcgcgtt ctgaccgagc
     2581 gcacgacgca cggtcatgta gtcgggtgcg tcgttgacgg agtcgatcgc gatgaggtgc
     2641 ccgtcgcggt agtagagcac cgagaagtgc tcgtcatcgg ggttgccgcg aagcacagct
     2701 tcatcgaagc cggtggagag acccgcgatc tgtagcttca ggtccgcctg gtctgaccag
     2761 aaccacggca cagctcgata gggctcaagc ttgccgatga gcgtcgccgc ggcgaccttc
     2821 gcttggtcga cggcattctg cacggactcg agacgtacac gcccttcacc gccaaggggg
     2881 tgcggaagca gcacggcatc tcccgcagcg acgacgcgag ggtcgctggt gcgagcgaac
     2941 tcatcaacga ctatcgcgcc gcccgagagc tcgagtccca gttgctcagc cagaccggag
     3001 cgaggaacca cgccgatacc aatcagcacc aggtcggagg ggatgagct
//
LOCUS       DAOSOU010000237         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_32303_length_3041_cov_4.891494, whole genome shotgun sequence.
ACCESSION   DAOSOU010000237 DAOSOU010000000
VERSION     DAOSOU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32303_length_3041_cov_4.891494"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLW15_10645"
     CDS             <1..454
                     /locus_tag="PLW15_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT34892.1"
                     /translation="VSEAVALNVDDVIDGDVVRLFGKGSKQRIVPVGSFAQAAIDAYL
                     VRARPAFSARGTATPALFLGARGQRVSRQNAWLIIRAAAERAQLGIEISPHTFRHSFA
                     THLLQGGADVRVVQELLGHSSVATTQIYTMVTADTLRDMYTTAHPRAR"
     gene            595..1482
                     /locus_tag="PLW15_10650"
     CDS             595..1482
                     /locus_tag="PLW15_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT34893.1"
                     /translation="MTVQREDSATLPGLETPVIGPTGRPQTIFPEPPPLRGHGPARII
                     SLCNQKGGVGKTTSTINLGAALAEYGRKVLAIDFDPQGALSAGLGVPTHDVPTIYDLL
                     LGTVKNPAEAIIHTAFANLDVIPANIDLSAAEVHLVNEVARETILARVLKKVSDQYDV
                     ILIDCQPSLGLLTVNALTAAHGVLIPLECEFFALRGVALLVETIDKVRDRLNPAIELD
                     GILATMYDARTLHSREVLERVVETFGDQVLETVIGRTVKFPDASVAGAPITSFAPDHS
                     AADAYRQLARELISRGAVA"
     gene            1466..2299
                     /locus_tag="PLW15_10655"
     CDS             1466..2299
                     /locus_tag="PLW15_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOT34894.1"
                     /translation="MAQSPSGAGLADGLETESSGFSVSLSNFNGPFDLLLSLIAKHEL
                     DITEVSLSKITDEFISYLRGLDSEEELDQATEFLVVAATLLDLKVAGLLPQGELVDAE
                     DVALLEARDLLFARLLQYRAFKEAAQWFSGRLDTESTRHFRSVRLEDKYRRSTPELVW
                     TLTPEDFAALAAYALAPRELPTVGLDHLHAPLVSIREQAAHVVALLRSGGTLTFRHLI
                     AGAEQKGVIIARFLAVLELYRAAAISFDQLEPLGELTIRWTAEHWSDDSLANLGADYG
                     N"
     gene            2289..2837
                     /gene="scpB"
                     /locus_tag="PLW15_10660"
     CDS             2289..2837
                     /gene="scpB"
                     /locus_tag="PLW15_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOT34895.1"
                     /translation="METERALEAILMVADEPQSLVSLATALSVPVPAVRQSIERLVAD
                     FDGADGATRRGFELREVGGGWRIYVRPEHDDVVRDFVLTQNPTRLSQAALETLAVIAY
                     KQPISRGSIASIRAVNVDSVVRTLLGRGLITEAFTDNETGAIHYETTDLMLTQLGINS
                     IDELPKISPLLDDGSQGFDEQR"
     gene            2824..>3041
                     /locus_tag="PLW15_10665"
     CDS             2824..>3041
                     /locus_tag="PLW15_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOT34896.1"
                     /translation="MNSADLDPSGASAEGERLQKVMAAAGVASRRVSEDLIAQGRVTV
                     NGEIVTEPGRRVLPADLITVDGTAIQTDT"
BASE COUNT          489 a         1087 c          983 g          482 t
ORIGIN      
        1 agtctcggag gccgtcgccc tcaacgtcga cgacgtcatc gacggtgacg tcgtacggct
       61 gttcggcaag ggcagcaagc agcgcatcgt tccggtgggc agtttcgcgc aggccgcgat
      121 cgatgcctac ctcgtgcgtg cccgaccggc cttctcggcg cggggcacgg cgacccccgc
      181 cctgttcctg ggggcgcggg ggcagcgggt gtcgcgccag aacgcgtggc tgatcatccg
      241 cgcggccgcc gagcgtgccc aactgggcat cgagatctcg ccgcacacgt tcaggcactc
      301 cttcgcgacc cacctgctgc agggcggcgc cgatgtgcgc gtggtacagg aactgctcgg
      361 tcactcgagc gtcgcgacga cgcagatcta cacgatggtg accgccgaca ccctgcggga
      421 catgtacacg acggcgcacc cgcgggcacg gtaatcgtgc tccggtgatc gagcgagtcg
      481 agcggtgatc gagcgaagtc gagattctaa ccctctacct gaactagagg tgagcacgcc
      541 cgcgagtgcg tgtgcgtcgc ggacagtcac agccacccgc cgtacacttt ccccatgaca
      601 gtgcagcgtg aggactcggc aaccctgccg ggactcgaga cgcccgtcat cggacccacg
      661 ggtcgtccgc agaccatctt cccggagccg ccaccgctgc gcggccacgg cccggcgcgc
      721 atcatctcgc tgtgcaacca gaagggtggc gtcggcaaga cgacgagcac catcaacctg
      781 ggtgcggcgc ttgccgagta cggccgcaag gtgctcgcga tcgacttcga cccgcagggc
      841 gcactctcgg cgggtctcgg cgttccgacc cacgacgttc ccacgatcta cgacctgctg
      901 ctcggcacgg tgaagaaccc cgccgaagcg atcatccaca ccgcctttgc caacctcgac
      961 gtgatccccg cgaacatcga cctgtcggcg gccgaggttc acctcgtcaa cgaggtcgcc
     1021 cgcgagacga tcctcgcgcg ggtgctcaag aaggtcagcg accagtacga cgtcatcctg
     1081 atcgactgcc agccgtcgct cggcctgctc accgtcaacg cgctcaccgc ggcgcacggt
     1141 gtgctcattc cgctcgagtg cgaattcttc gcgctgcgcg gcgtggctct gctcgtggag
     1201 acgatcgaca aggtgcgcga ccgcctcaac ccggcgatcg agctggatgg catcctcgcg
     1261 acgatgtacg acgctcgcac cctgcactcg cgggaagtgc tcgagcgcgt ggtcgagaca
     1321 ttcggtgatc aggtgctcga gaccgtcatc ggtcgcaccg tgaagttccc ggatgccagc
     1381 gtcgccggcg ccccgatcac gtcgttcgct cccgatcact cggccgccga cgcctatcgc
     1441 cagctcgcca gagagttgat ctcccgtggc gcagtcgcct agcggcgcgg ggctggcaga
     1501 cgggctggaa accgaatcct cgggtttctc cgtcagcctg agcaacttca acgggccgtt
     1561 cgacctgctg ctctcgctca tcgccaagca cgagctcgac atcaccgagg tgtcgctgtc
     1621 gaagatcacc gacgagttca tctcctacct gcgcgggctc gactccgagg aggagctcga
     1681 ccaggcgacc gagttcctcg tggttgccgc gacgctgctc gacctcaagg tggcggggct
     1741 gcttccccaa ggcgagctgg tcgacgccga ggatgtcgcc ctcctcgagg cgcgcgacct
     1801 gctgttcgcc cgactgctgc agtaccgcgc gttcaaggag gccgcccagt ggttctcggg
     1861 ccgcctcgac accgagtcga cccggcactt ccgctcggtg cggctggagg acaagtaccg
     1921 caggtcgaca cccgagctgg tgtggacgct cacgccggag gacttcgccg cgcttgcggc
     1981 gtacgccctc gcgccgcgag agctacccac cgtgggactg gaccatctgc acgcgcccct
     2041 ggtcagcatc cgggagcagg ccgctcacgt ggtcgcgctg ctgcgctccg ggggcaccct
     2101 gaccttccgc cacctgatcg ccggggccga gcagaagggc gtgatcatcg cgcgctttct
     2161 cgcggtgctg gagctgtacc gggcggccgc catctcgttc gaccagctcg agccgctggg
     2221 cgaactcacc atccgctgga ccgccgagca ctggtcggac gacagcctcg ccaatctggg
     2281 agccgactat ggaaactgag cgtgccctcg aggcgatcct catggttgcc gacgaaccgc
     2341 agagcctcgt gtcgctggcg accgcgctga gcgttcccgt gcccgccgtg cgccagtcga
     2401 tcgagcgact cgtcgccgac ttcgacggcg ccgacggcgc cacccgccgc gggttcgagc
     2461 tgcgcgaggt cggtggcgga tggcgcatct acgtgcgccc ggagcacgac gacgtcgtgc
     2521 gcgacttcgt gctcacccag aaccccacgc gcctgagcca ggccgcgctc gagaccctcg
     2581 cggtcatcgc gtacaagcag ccgatcagtc ggggttcgat cgcgtcgatt cgcgcggtca
     2641 acgtcgactc cgtcgtgcgc acgcttctcg gccggggact gatcaccgag gcgttcaccg
     2701 acaacgagac gggcgccatc cactatgaga cgacggacct gatgctcacc cagctcggaa
     2761 tcaactcgat cgacgagctt ccgaagatct cgccgctgct cgatgacgga tcgcagggct
     2821 tcgatgaaca gcgctgacct ggacccgtcc ggcgcctcgg ccgagggcga gcgcctgcag
     2881 aaggtgatgg ccgccgccgg cgtggcatcc cgtcgcgtct cggaggacct catcgcccag
     2941 ggccgggtca cggtcaatgg cgagatcgtc accgagccgg gccgccgcgt gctgcccgcc
     3001 gatctgatca ccgtcgacgg caccgcgatc cagaccgaca c
//
LOCUS       DAOSOU010000238         3037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_32363_length_3037_cov_4.982562, whole genome shotgun sequence.
ACCESSION   DAOSOU010000238 DAOSOU010000000
VERSION     DAOSOU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32363_length_3037_cov_4.982562"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLW15_10670"
     CDS             complement(<1..739)
                     /locus_tag="PLW15_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT34897.1"
                     /translation="MSDGPIDLHTHSSVSDGTETPTQLVRAAVEAGLGTVALTDHDST
                     AGWHEAFAAAAGTGLTVIPGMELSTNWGPASVHVLAYLFDPNNGDIVAETARIRDGRL
                     HRAEQIVERIAADYDLTWDDVLAESSDGTTLGRPHIADALVRKGFVPNRTAAFESILH
                     WRGGYYVKYYAPSPVEGVRTIVAAGGVAVLAHPVTSNKYRPMDDTVIRQLVDVGLFGL
                     ELDHRDNTVEGKARLRTLAKKFGLELTG"
     gene            772..1785
                     /locus_tag="PLW15_10675"
     CDS             772..1785
                     /locus_tag="PLW15_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT34898.1"
                     /translation="MFARLLPALVTVGIAITALVLAWPQLFGVARAPVVAQVVALRGL
                     GIVVALVLVVTFTLFALLAPAARRFLASLAVVLLAFCAINAAVLATRGFGNMAFETAN
                     DATVTVLSWNTLGDAPGSAVIAGLALESGADVVALPETTRATGEEVAALMAEAGHPMQ
                     VLTVAYDEVSKARSTTLLIGNDLGDYDVDDTVPTTAQLPSIVATPADGSGPTIIAVHT
                     LAPIPPMMAAWESDLRWAASACSGDNVIMAGDFNATLDHFGGLLHAEGATLGNCADAA
                     LATDNAAVGTWPTMLPALIGAPIDHVLATPNWRVSGMRVIASHDGYGSDHRPILVQLS
                     PAG"
     gene            1796..>3037
                     /locus_tag="PLW15_10680"
     CDS             1796..>3037
                     /locus_tag="PLW15_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOT34899.1"
                     /translation="MAESTTPAPRATSNRSTTPVSETFAQYIASQWAERPDVLPSPRE
                     QASYAAERRSRISAMHPGKRLVVPAGAAKTRSNDTDYAYRAHSAFAWLTGWGSDSVAG
                     SVLVLEPTADGHDATLYFREAAGRDTDEFYANPEIGEFWTGPRPSLEHVAADLGLTTR
                     TLAAFSDVLDATDAATLVVRDADRDLTDQIDGRRLVTAESADPETLDTSGDAELARDL
                     SELRLVKDEFELAEMRAAVAATKLGFDDVIGDLSTIVAHERGERLVEGTFNRRARAEG
                     NTVGYDTIAASGPHACILHWTRNDGPVVPGDLILIDAGIELDSYYTADITRTLPVSGT
                     FMPVQRRVYEAVREAADAAFAIVRPGIKFREIHAAAMQVIAARTAEWGLLPVTAEESL
                     EPDQQYHRRYMVHGTSHHLGLD"
BASE COUNT          438 a         1105 c         1019 g          475 t
ORIGIN      
        1 acccggtgag ctcgagaccg aacttcttcg ccagtgtccg caggcgggcc ttgccctcga
       61 cggtgttgtc gcggtggtcg agttccagac cgaagagacc cacatcgacg agttggcgga
      121 tgaccgtgtc atccatgggc cggtacttgt tgctggtgac cggatgcgcc agcacggcca
      181 ccccgccggc cgcgacaatc gtgcgcaccc cctcgacggg cgacggcgcg tagtacttca
      241 cgtagtagcc gccgcgccag tgcaggatcg actcgaacgc cgccgtgcgg ttgggcacga
      301 agcctttgcg cacgagcgcg tcggcgatgt gcgggcgacc gagagtggtg ccgtcggagg
      361 actcggcgag cacgtcgtcc caggtgaggt cgtagtcggc cgcgatgcgc tccacgatct
      421 gctcggcccg gtgcaggcgg ccgtcgcgga tgcgcgccgt ctccgcgacg atgtcgccgt
      481 tgttggggtc gaagaggtag gccagcacgt gcacgctcgc cggcccccag ttggtgctga
      541 gctccatgcc cgggatgacc gtgagcccgg ttcccgcggc agccgcgaac gcttcgtgcc
      601 agcctgcagt ggagtcgtga tcggtcagcg cgacggttcc caggcccgcc tccacggcgg
      661 cgcgcaccag ctgcgtcggc gtctcggtgc cgtccgaaac cgaactgtgc gtgtgcaggt
      721 cgatcgggcc gtcggacatc cctcaagact attggtctaa acttcggcag gatgtttgcc
      781 cgcctgctgc ccgccctcgt gaccgtgggc atcgccatca ccgctctggt gctcgcctgg
      841 ccccaactgt tcggcgtcgc gcgcgcgccg gtcgtcgctc aggtcgtcgc gctgcgaggg
      901 ctgggcatcg tcgtcgccct cgtgctcgtc gtcacgttca ccctgttcgc gctgctggcg
      961 ccggccgccc gccgctttct ggcgtcactt gccgttgtgc tgctcgcgtt ctgtgcgatc
     1021 aacgccgcgg tgctggcgac gcgcggattc ggcaacatgg ccttcgaaac ggcgaacgat
     1081 gcgacggtta cggtgctctc gtggaacacc ctcggggatg ctccggggtc ggcggtgatc
     1141 gccggcctcg cgctcgagtc cggggccgac gtggtggccc tgccggagac gacgcgggcc
     1201 acgggtgagg aggtcgctgc gctcatggct gaggccgggc atccgatgca ggttctgacg
     1261 gtcgcgtacg acgaggtctc gaaggcccgc tcgacgaccc tgctgatcgg caacgatttg
     1321 ggcgactatg acgtggatga cacggtgccc accaccgccc agctgccgtc gatcgtcgcc
     1381 acgccggcag acggctcggg tccgacgatc atcgccgtgc acacgctcgc ccccatcccg
     1441 ccgatgatgg cggcctggga gagcgacctc aggtgggcgg catccgcctg ctcgggcgac
     1501 aacgtgatca tggcgggcga cttcaacgcg acgctcgacc acttcggcgg gctgctgcac
     1561 gccgaagggg caaccctggg caactgcgcc gacgccgctc tcgccaccga caacgccgcc
     1621 gtcggcacct ggccgacgat gctgcccgcg cttatcggtg cgcccatcga ccacgttctg
     1681 gcgacaccga actggcgcgt cagcggcatg cgggtgatcg cctcgcacga cggctacgga
     1741 agcgaccacc ggccgatcct cgtgcagttg tcgcccgcgg ggtgagaatg gtgtcatggc
     1801 agaatccacg actccggccc cccgcgcgac ctcgaaccgc tccaccaccc cggtgagcga
     1861 gaccttcgcg cagtacatcg cctcgcagtg ggcggagcgg ccggacgtgc tgccgtcgcc
     1921 ccgcgagcag gcgtcgtacg ccgcagagcg ccgttcgcgc atctcggcga tgcatccggg
     1981 taagcgcctc gtcgtcccgg cgggcgcggc caagacccgc tcgaatgaca cggactacgc
     2041 gtatcgcgca cactccgcct tcgcgtggct caccgggtgg ggctccgact cggtcgccgg
     2101 cagcgtgctt gtgctggagc cgactgccga cggccacgac gccaccctct acttccgcga
     2161 ggcggccggc cgcgacaccg acgagttcta cgccaacccc gagatcggcg agttctggac
     2221 cggcccgcgc ccctcgctcg agcacgtcgc cgccgacctg gggctcacca ctcgcaccct
     2281 cgctgcgttc agcgatgtgc tcgacgcgac ggatgccgcg accctcgtcg tgcgcgacgc
     2341 cgaccgcgat ctcaccgacc agatcgacgg ccgccgcctg gtcactgccg agtcggcgga
     2401 ccccgagact ctcgacacct ccggcgacgc cgaactcgcg cgcgacctca gtgagctgcg
     2461 cctcgtcaag gacgagttcg agctcgccga gatgcgcgcc gcggtcgccg ccaccaagct
     2521 cggcttcgac gacgtgatcg gcgacctgtc cacgatcgtc gcgcacgagc gcggggagcg
     2581 cctcgtcgag ggcacgttca accggcgtgc gcgggccgag ggcaacaccg tgggttacga
     2641 cacgatcgcg gcatccggac cgcacgcgtg cattctgcac tggacccgca acgacggccc
     2701 cgtcgtgccg ggtgacctga tcctcatcga cgccggcatc gagctcgaca gctactacac
     2761 cgccgacatc acccgcacgc tgcccgtcag cggcaccttc atgcccgtgc agcgccgcgt
     2821 ctacgaggcg gtgcgcgagg cagcggatgc cgcgttcgcg atcgttcggc ccggcatcaa
     2881 gttccgcgag atccacgccg ccgccatgca ggtcatcgcg gcgcgcaccg ccgagtgggg
     2941 actgctgccg gtcaccgcgg aggagtctct cgagcccgat cagcagtacc accgccgcta
     3001 catggtgcac ggcacgagcc atcacctggg tctcgat
//
LOCUS       DAOSOU010000239         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_32624_length_3017_cov_6.453072, whole genome shotgun sequence.
ACCESSION   DAOSOU010000239 DAOSOU010000000
VERSION     DAOSOU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32624_length_3017_cov_6.453072"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLW15_10685"
     CDS             complement(<1..375)
                     /locus_tag="PLW15_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOT34900.1"
                     /translation="MSAEAPVSPGHLADRVPTLSGEEMVAQLVPPRQFATATLDSYRP
                     DRDYPSQGAAVAAIRNFVGGWQSGGGGLFSRKKAAPALPGVYLDGGFGVGKTHLLAAL
                     WHVAPGRKYFGTFIEYTALVGAL"
     gene            514..1413
                     /locus_tag="PLW15_10690"
     CDS             514..1413
                     /locus_tag="PLW15_10690"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOT34901.1"
                     /translation="MTVEVDTAPQFSAFAHPERLVSGDWLEAHLGTPGLVVVESDEDV
                     LLYETGHIEGAVKIDWHTDLNDPVERDYIDGAAFAKLVGAKGIGRDTTVVIYGDKNNW
                     WAAYALWVFTLFGHEDVRLLDGGRAKWEADGRAYTTAEPEITPVDYPVVERNDAAIRA
                     FKEDVIAHLGNPLIDVRSPEEYSGERTHMPAYPEEGALRGGHIPSAQSVPWARAVAED
                     GTFKSLPELDAIYREGAGLKDGDSIVAYCRIGERSSHTWFVLTHLLGFEGVRNYDGSW
                     TEWGSAVRVPIAKGTEPGAVPAK"
     gene            1441..1893
                     /locus_tag="PLW15_10695"
     CDS             1441..1893
                     /locus_tag="PLW15_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOT34902.1"
                     /translation="MTTEDSAAELPEQLTQIREDFLALAVRERLQLLLEFSNELPELP
                     ERYLDHPDLLERVEECQSPVFIFVEVDDASIVHLYATAPKEAPTTRGFASILAQGLAG
                     LTAEQVLAVPDDYPNTIGLTEAVSPLRIRGMTALLGRTKRQVREKLVA"
     gene            1890..2600
                     /locus_tag="PLW15_10700"
     CDS             1890..2600
                     /locus_tag="PLW15_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT34903.1"
                     /translation="MILTRVHPGPPESLELDAASTRDRLLNLYRPGPSPTLRLNLVAS
                     ISGSAAGSDGTSDTLTSRVDRRILGVIRELSDVVLVGAASVRAEGYQVPQRARLAVLT
                     ASGDLAGHRIRPDQRGSVIVLAPAEARERVLESLPGAELIIVPTTGQTIAMNHVVDAL
                     HSAGFPSIVCEGGPSLAGQLIAAGLVDELCLTTSPQLGGVTLPLLATAPASRAATLTQ
                     LIVDQQGYLFARWALRAR"
     gene            complement(2530..>3017)
                     /locus_tag="PLW15_10705"
     CDS             complement(2530..>3017)
                     /locus_tag="PLW15_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT34904.1"
                     /translation="LQALTRSSSADRVSGVILESPVVDWVTALDFQGKVNRLPSALRW
                     GVLQLLSHRWAGRLTGQSEPIDLRRLDLVARADELDKPILLFHSEDDGYVPATASTAL
                     ARARPDIVTFEHFMTARHVRLWNYDVARWNSAITAWLSALAAPTGRTGSPAGRRSVAS
                     E"
BASE COUNT          521 a         1051 c          980 g          465 t
ORIGIN      
        1 tagggctccc acgagcgcgg tgtactcgat aaaggtgccg aagtacttgc gcccgggagc
       61 aacgtgccac agtgccgcga gcagatgggt cttgcccacc ccgaagccgc cgtcgagata
      121 gacacccggc agggcgggcg ccgccttctt gcgggagaac agtccgccac ccccggactg
      181 ccagcccccg acgaagttgc ggatcgcggc taccgcggca ccctgtgacg gatagtcccg
      241 gtcaggtcgg taggaatcga gggtcgccgt cgcgaactgg cgcggtggca ccagctgtgc
      301 caccatctcc tcgccgctga gcgtgggcac gcggtcagca agatgcccgg gtgagacggg
      361 cgcttcggca ctcatggatc agcctcggaa atttgtgtcg aagtcttacg gtgccccggt
      421 gcggatgcac gcggactctc gtagattcgt gagcaagggg attccagcct agccttggca
      481 gccctgcaca cgatccacaa gctcggagga accatgaccg tcgaagtcga taccgcaccc
      541 cagttctcgg ccttcgccca ccccgagcga ctcgtctccg gggactggct cgaggcgcac
      601 ctcggcactc ccggcctcgt cgttgtcgaa tccgacgaag acgtgctgct gtacgagacg
      661 ggccacatcg agggcgccgt gaagatcgac tggcacaccg acctcaacga cccggtcgag
      721 cgcgactata tcgacggagc cgccttcgcg aagctcgtcg gagccaaggg catcggtcgc
      781 gacacgacag tggtcatcta cggcgacaag aacaactggt gggccgcgta cgccctctgg
      841 gtcttcaccc tgttcggcca cgaggatgtc cgcctcctcg atggcggccg cgccaagtgg
      901 gaggctgacg gacgcgccta caccacggcc gagccggaga tcacgcccgt cgactacccc
      961 gtcgtggaac ggaatgacgc ggccatccgc gcattcaagg aggacgtgat cgcccacctc
     1021 ggcaacccgc tcatcgacgt gcgctccccc gaggagtaca gcggcgagcg cacccacatg
     1081 cccgcctacc ccgaagaggg cgcgctgcgc ggcggtcaca tccccagcgc acagagcgtg
     1141 ccgtgggcaa gggccgttgc cgaggacggg accttcaagt cgctccccga gctcgacgca
     1201 atctaccgcg agggtgctgg gctcaaggac ggcgacagca tcgtcgccta ctgccgtatc
     1261 ggcgagcgtt cgagccacac ctggttcgtg ctgacgcacc tgctgggctt cgagggcgtg
     1321 cgcaactacg acggctcctg gaccgagtgg ggatcggcag tgcgcgtgcc gatcgccaag
     1381 ggcaccgagc cgggcgctgt accggccaag tagatctccc tccacaccta gaatcgtcag
     1441 gtgaccaccg aggacagcgc ggcagaactc ccggagcaac tcactcagat ccgtgaggac
     1501 ttcctcgcgc tcgcggtgcg ggagcgtctc cagttgctcc tcgagttctc gaacgagctg
     1561 ccggagttgc ccgagcgcta cctcgaccac cccgacctgc tcgagcgtgt cgaggagtgc
     1621 cagtcgcccg tcttcatctt cgtcgaggtg gatgacgcga gcatcgtgca cctctacgcc
     1681 accgcaccga aggaggcgcc gacgactcgg ggcttcgcat cgatcctcgc gcagggactg
     1741 gccggcctca ccgccgagca ggttctcgcg gtgcccgacg actaccccaa caccatcggc
     1801 ctgactgagg cagtgagccc gctgcgcatc cgcgggatga ccgcgctgct gggtcgcacg
     1861 aagcggcagg tgcgggagaa gctcgtcgcg tgatactcac gcgggtgcat ccggggccgc
     1921 ccgagtccct ggaactggat gccgcatcca cgagagaccg cctgctcaac ctctaccgtc
     1981 ccgggccgtc gcccaccctg cgcctcaacc tcgtggcgag catctcgggc agcgcggcgg
     2041 ggtcggacgg cacctccgat acgctgacca gccgggtcga tcggcgcatc ctgggcgtca
     2101 tccgggaact cagtgacgtc gtgctggtcg gcgccgcctc ggtcagggcc gagggctatc
     2161 aggtacccca gcgtgcccgc cttgcggtgc tgaccgcgag tggcgatctt gctggacatc
     2221 gcatacgtcc cgaccaacgc gggagtgtga tcgtcctcgc ccccgccgaa gcgcgcgaac
     2281 gagtgctcga atcgcttccc ggggccgaac tcatcatcgt gcccaccacc gggcagacga
     2341 ttgcgatgaa ccatgttgtc gacgcgctgc actccgctgg gttcccctcg atcgtctgcg
     2401 aggggggacc gagcctcgcc ggtcagctca tcgccgcggg cttggtcgac gaactctgcc
     2461 tcaccacgag ccctcagctc ggcggcgtca ccctcccgct gctggcgacc gcgccggcgt
     2521 ctcgcgccgc tactctgacg caactgatcg tcgaccagca gggctacctg ttcgcccggt
     2581 gggcgctgcg agcgcgctga gccatgctgt gatcgccgag ttccagcgcg ccacgtcata
     2641 gttccacaac ctcacatgcc gagcggtcat gaagtgctcg aaggtgacga tgtcggggcg
     2701 ggcccgggcg agagccgttg acgcggtcgc aggcacgtag ccgtcatctt cgctatggaa
     2761 gagcaggatc ggcttgtcga gctcgtcggc gcgcgcgacg aggtcgagcc gacgaaggtc
     2821 gatcggctca ctctgaccgg tgagacgacc cgcccagcgg tgtgacagca actggagcac
     2881 gccccagcgc agcgcggacg gcaggcgatt gaccttgcct tgaaagtcaa gcgccgtcac
     2941 ccagtcgacg accggcgatt caaggatgac gccgctcacc cgatccgccg aggacgaccg
     3001 cgtgagtgcc tgaagca
//
LOCUS       DAOSOU010000240         3011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_32714_length_3011_cov_6.328146, whole genome shotgun sequence.
ACCESSION   DAOSOU010000240 DAOSOU010000000
VERSION     DAOSOU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32714_length_3011_cov_6.328146"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLW15_10710"
     CDS             <1..386
                     /locus_tag="PLW15_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34905.1"
                     /translation="TLANTNAPADPNFLVNTIITALTGTGNFFGQFATTLTNLLNAFN
                     ISSDDITGSAFYVGVGSSGALGGIVDGVTSGVAFPAVAIPAVVVPPDIGGGSVFSLGT
                     GTKNFTNNFTNGGVDGQYTVTYTFG"
     gene            517..2316
                     /locus_tag="PLW15_10715"
     CDS             517..2316
                     /locus_tag="PLW15_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT34906.1"
                     /translation="MSDYRAPIKDALFTLRHIGEIEQLAATERYAHADLDTVSSVLEE
                     QGRFMQEVFGPLNHSGDVEGLKLADGVVTTPAGFKDAYAKFVAAGWQGLNAEEEFGGA
                     GFPEAVFSVALEFMTAANGALTMCPGLTTGAIECIQHWGTEEQKEVYLRKLVTGEWTG
                     TMNLTEPQAGSDVGLCTTKAEPQGDGTYKISGTKIFISFGEHDMAENIIHLVLARIPG
                     SPPGTKGISLFIVPKFLVNGDGSLGERNDVTCVSIEHKMGIHASPTCVLSYGDDGGAI
                     GYLIGEEGQGMRAMFTMMNSARLAVGIQGVAIGDAAYQKALQYSQERKQGKEIGSASH
                     EPAFIIQHPDVRRMLMTMRSQIDAMRSLIVYNAAAIDFSRSHADPELREQWREIAELL
                     TPVTKAWCTDVGMDVTSTAVQVFGGMGFIEESGVAQHFRDMRIAPIYEGTNGIQAMDL
                     VGRKLPMRMGGVANDHFARIAATIAELEATTELKALAAPLQAGLESLQAVTNWIFENG
                     LADPKEALSGATPYLEMFGVVTGGWFSARMALAAQGELNDGSDDVAYLKARISSAAFF
                     IEQLVPRAAGLAASVTAGASGLYEIDAADLASI"
     gene            2416..>3011
                     /locus_tag="PLW15_10720"
     CDS             2416..>3011
                     /locus_tag="PLW15_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34907.1"
                     /translation="MLDHVFTDAIGALRDALEKALLERQAFEERFQADVLLGDLTWET
                     SYSLPGEGSPPRIRCDITLDWPTWSQTAYRSWYLEEELVDPPHIDIEVVLRIQRLSTQ
                     ADPKIVLAALPLESPPIGPERLRRGGPTLESTYGDDLDDVEHAIEVSYEGSYELDEQS
                     LADGSILDDHFSAMGGWISATLVRLGDLSMAHLPPMID"
BASE COUNT          520 a         1109 c          920 g          462 t
ORIGIN      
        1 cgacgctggc caacaccaac gcccccgccg atcccaactt cctcgtcaac accatcatca
       61 cggcgttgac cggcaccggg aacttcttcg gccagttcgc gacgacgctc accaacctgt
      121 tgaacgcgtt caacatctcg agtgacgaca tcacgggcag tgccttctac gtcggcgtcg
      181 ggtcctccgg cgccctcggc ggcatcgtcg acggcgtgac cagcggcgtg gccttcccgg
      241 ccgtggcgat ccccgccgtc gtcgttccgc cggacatcgg cggcggctcg gtcttctcgc
      301 tcggcacggg cacgaagaac ttcacgaaca acttcaccaa cggtggcgtc gacggtcagt
      361 acaccgtcac ctacaccttc ggctgaccaa ccggtcaccc cgaactgcag cgtgccccgg
      421 gctccggccc ggggcacgtg cgcgttccgg cagcaccccg ccacgaccgg agcgcgcgac
      481 cttgcacgaa agttacccgc gggtaacata gaacccatgt ctgactaccg ggcccccatc
      541 aaggacgcac tgttcaccct ccgccacatc ggcgagatcg aacagctcgc cgccaccgag
      601 cgctacgccc acgccgacct cgacaccgtg tcatcggtcc tcgaggagca gggccgcttc
      661 atgcaagagg tcttcggtcc gctgaaccac agcggcgacg tcgaggggct caagctggct
      721 gacggtgtcg tcacgacgcc cgccggtttc aaggatgcgt acgccaagtt cgtcgccgcc
      781 ggctggcagg gcctcaacgc cgaagaggag ttcggcggcg ccggattccc cgaggccgtg
      841 ttctccgttg cactcgagtt catgacggcc gcgaacggtg ccctcacgat gtgccccggg
      901 ctcaccaccg gcgccatcga gtgcatccag cactggggca ccgaggagca gaaggaggtc
      961 tacctccgca agctggtgac cggcgagtgg accggcacga tgaacctcac cgaaccgcag
     1021 gccggctccg acgtcggctt gtgcacgacg aaggccgagc cccagggcga cggcacgtac
     1081 aagatctcgg gcaccaagat cttcatcagc ttcggcgagc acgacatggc cgagaacatc
     1141 atccacctgg tcctcgcccg catccccggc tcgcctcccg gcaccaaggg catctcgctc
     1201 ttcatcgtcc ccaagttcct cgtcaacggc gacggcagcc tcggcgagcg caacgacgtc
     1261 acgtgtgtgt cgatcgagca caagatgggc atccacgcct cccccacctg tgtgctgagc
     1321 tacggcgacg acggcggcgc catcggctac ctgatcggcg aagagggcca gggcatgcgc
     1381 gccatgttca cgatgatgaa cagcgcccgc ctcgccgtcg gcatccaggg tgtggccatc
     1441 ggcgacgccg cctaccagaa ggcgctccag tacagccagg agcgcaagca gggcaaggag
     1501 atcggcagcg ccagccacga gccggcgttc atcatccagc accccgacgt tcgccgcatg
     1561 ttgatgacga tgcgcagcca gatcgacgcg atgcgctcgc tgatcgtcta caacgccgct
     1621 gcgatcgact tctcccgcag ccacgccgac cccgagctcc gcgagcagtg gcgcgagatc
     1681 gccgagctgc tcacgccggt caccaaggcg tggtgcaccg acgtcggcat ggacgtcacc
     1741 tcgaccgccg tccaggtgtt cggcggcatg ggtttcatcg aggagtcggg tgtcgcccag
     1801 cacttccgcg acatgcgcat cgccccgatc tacgagggca ccaacggcat ccaggccatg
     1861 gacctcgtcg gtcgcaagct gccgatgcgt atgggcggtg tcgccaacga ccacttcgcc
     1921 cgcatcgcgg cgaccatcgc cgaactcgaa gccaccaccg agctgaaggc gctcgcagcg
     1981 ccgctgcagg ccggcctcga gtcgctccag gcagtcacca actggatctt cgagaacggc
     2041 ctcgccgacc cgaaggaagc tctctcgggt gcgacgccct acctcgagat gttcggcgtc
     2101 gtgaccggcg gctggttctc cgcccgcatg gcgctcgccg cccagggcga actcaacgac
     2161 ggttccgacg acgttgcgta cctcaaggct cgcatctcgt cggccgcgtt cttcatcgag
     2221 cagctggtcc cccgggccgc cggcctcgca gcgtcggtca ccgccggtgc gtcgggcctc
     2281 tacgagatcg acgccgcgga cctcgcgtcg atctgacctc gaactgacac tgcggtcgct
     2341 gtcccgcgga tccttcgaac tcccgaagga tccgcgggac agcgcgcgtc agggcaccag
     2401 ttagcctggt ggcacatgtt ggaccacgtc ttcaccgatg ccatcggcgc cctccgcgac
     2461 gccctcgaga aggccctgct cgaacggcag gcgttcgagg agcggttcca ggccgatgtc
     2521 ctgctcggcg acctcacctg ggagacctcg tactcgctcc ctggggaggg gtcgccgccg
     2581 cgcatccgct gcgacatcac cctcgactgg cccacgtggt cgcagacggc gtaccgctcc
     2641 tggtacctcg aggaagagct ggtcgacccg ccccacatcg acatcgaggt cgtcctgcga
     2701 atccaacggc tgtcgaccca ggccgacccc aagatcgtgc tcgcggcgct cccgctcgaa
     2761 agcccgccga tcggtcccga gcgacttcgt cgtggcgggc ccacgctgga gtcgacgtac
     2821 ggcgacgacc tcgacgacgt cgagcatgcg atcgaggtgt cctacgaggg gtcgtacgag
     2881 ctcgacgagc agagcctggc cgacgggtcc atcctcgacg accacttctc cgccatgggc
     2941 ggttggatca gcgccaccct ggtgcgcctc ggcgacctct cgatggcgca cctgccaccg
     3001 atgatcgacg a
//
LOCUS       DAOSOU010000241         3008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_32748_length_3008_cov_7.079241, whole genome shotgun sequence.
ACCESSION   DAOSOU010000241 DAOSOU010000000
VERSION     DAOSOU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32748_length_3008_cov_7.079241"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1624
                     /gene="purF"
                     /locus_tag="PLW15_10725"
     CDS             167..1624
                     /gene="purF"
                     /locus_tag="PLW15_10725"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT34908.1"
                     /translation="MCGIVGIVSSHPVNQQVYDALALLQHRGQDSTGIATAEGSIIHM
                     FKAKGQVREAYRTRDMRTLLGNVGLGHVRYATKGSASSEEEAQPFYVNAPYGIVLVHN
                     GNLTNTRELTQELFHIDRRHLNTSSDTELLVNVLANELQEQITGTDLDPDQVFAAISR
                     LHERVEGSYAAIALIAGHGLLAFRDPFGIRPLVLGRRNADFVGAEWVVASESLVLESA
                     GYEVVRDVAPGEAIFITPNGEMHSRQCAANPRLVPCSFEYVYLARPDSVMNGISVYEA
                     RLRLGDRLADTIARYTPQGDIDVVMPIPDSSRPAAMQVAQKLGVEYREGFYKNRYVGR
                     TFIMPGQAERKKSVKQKLNAMSSEFKGKNILIVDDSIVRGTTSREIVQMAREAGANKV
                     TFTSAAPPVRFPHVYGINMPSRHELVAHGRKIPEIATELGADYLIYQEVADMQAAIIE
                     GSDVTALDMSCFTGDYVTGTVTAEYLDWVERTQLS"
     gene            1915..2376
                     /locus_tag="PLW15_10730"
     CDS             1915..2376
                     /locus_tag="PLW15_10730"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HOT34909.1"
                     /translation="MSNQWRRVAAALDNPDRRRVYAEAVLGLAGEPSTRRKKSLAALR
                     EAGLLDAAGAPIADAFARLLAETAPVTRQGLERWVTAGVITEWPSRPADRDELLAWVL
                     ERAAEPGESLGERELTERLAALGHDPVALRRALVDAELVTRTVDGSTYRRS"
     gene            complement(2373..2687)
                     /locus_tag="PLW15_10735"
     CDS             complement(2373..2687)
                     /locus_tag="PLW15_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Trk"
                     /protein_id="HOT34910.1"
                     /translation="MSEPESTVKIRRAPKIGAFLVVGGLIGALVTLVLTAQFPVDPNV
                     GFIALFGYFCLFGIPAGVLLGALLALLLDRVSRRRAREVVAERESVGGESVAGEGVAD
                     ES"
     gene            complement(2684..3007)
                     /locus_tag="PLW15_10740"
     CDS             complement(2684..3007)
                     /locus_tag="PLW15_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HOT34911.1"
                     /translation="MTAALAGSKPDLATATRYLLQVLAENAEGNTVEVRVPPFGAVQA
                     VEGPRHTRGTPPNVIEMDAPTWLALATGGTTWALAVASGAVSASGARADLAPYLPLAW
                     ETMEG"
BASE COUNT          502 a         1074 c          998 g          434 t
ORIGIN      
        1 actccggcgt cacggtagct gcgggaggag tcctcggtca cccgaccagc gtacccgggt
       61 gtcgcgcggt gaaccggatc ggccgacctg cggcaccggt atgggagact tgaccgtacc
      121 cgcttcgggt gcgcgccctt cgagcatcag ggagcaaccg gccagtatgt gcggcatcgt
      181 cggaatcgtc tcgtcccatc cggtcaatca gcaggtctac gacgcactcg cgctgctgca
      241 gcaccgggga caggactcca ccggcatcgc gaccgccgag ggcagcatca tccacatgtt
      301 caaggcgaag gggcaggtgc gcgaggcata ccgcacccgc gacatgcgca ccctcctcgg
      361 caatgtgggc ctcggccacg tgcgctatgc caccaagggc agcgcgtcga gcgaggagga
      421 ggcacagccc ttctacgtga atgcgcccta cggcatcgtg ctggtgcaca acggcaacct
      481 cacgaacacc cgcgagctca cgcaggagct cttccacatc gaccgtcgtc acctcaacac
      541 gtcgagcgac accgagctgc tggtcaacgt gctcgccaac gagctgcagg agcagatcac
      601 cggaaccgac ctcgaccccg atcaggtctt cgcggcgatc tcgcggctgc acgagcgggt
      661 cgagggctcc tacgcggcga tcgcgctgat cgccggtcac ggcctgctgg cgttccgcga
      721 tccgttcggc atccggccgc tggtgctggg gcgccgcaat gctgacttcg tcggggccga
      781 gtgggtcgtg gcatccgaat cgctggtgct ggagagcgcg ggctacgagg tggttcggga
      841 tgtcgcgccg ggcgaggcca tcttcatcac ccccaacggc gagatgcact cgcgccagtg
      901 cgccgcgaac ccccgtctgg tgccgtgctc gttcgagtac gtctacctcg cccgacccga
      961 ctcggtgatg aacggcatca gcgtgtacga ggcgcgcttg cggctcggcg accgcctcgc
     1021 cgacacgatc gcgcggtaca ccccgcaggg cgacatcgac gtggtcatgc cgattccgga
     1081 ctcctcgcgc cccgccgcga tgcaggtcgc ccagaagctc ggcgtcgagt accgcgaggg
     1141 cttctacaag aaccgctacg tcgggcgcac gttcatcatg cccgggcagg cggagcgcaa
     1201 gaagagcgtc aagcagaagc tcaacgcgat gtcgagcgag ttcaagggca agaacatcct
     1261 gatcgtcgac gactcgatcg tgcgcggcac cacctcgcgc gagatcgtgc agatggcgcg
     1321 cgaggcgggc gccaacaagg tgaccttcac gtcagccgcg ccgccggtgc gctttccgca
     1381 cgtgtacggc atcaacatgc cgtcgcggca cgagctcgtg gcccacggcc gcaagattcc
     1441 cgagatcgcc accgagctgg gcgccgacta cctgatctac caggaggtcg cagacatgca
     1501 ggccgccatc atcgagggct cggatgtcac ggccctcgac atgagctgct tcacgggcga
     1561 ctacgtgacc ggcaccgtca ccgccgagta cctcgactgg gtggaacgca cgcagctgag
     1621 ctgagcgagc tcaacgggcc gcttcctggg tcggggaatc cacctccagg ccgtgtccac
     1681 gcgccaccgc tcgacgcgcc accgctcgac gctcgccgcc cgcccgcggc tgctccttcc
     1741 cagcgcgctt gcccatacct ccggtgaatc gttccccgaa ctcaggacat tcgtagaggt
     1801 gcccaacaat tgtcctgagt tcggggaacg aatgggaggg gtcgttcaca acccacggag
     1861 gcgccaccgt tcggtgccag cccgccacgg ccagcacatc ggtaccgttg agccatgagc
     1921 aaccagtggc gccgggtggc cgcggccctc gacaatcccg accgacggcg ggtgtacgcc
     1981 gaggccgtgc tgggactcgc gggcgaaccg tcgacgcgtc gcaagaagtc gctcgcggca
     2041 ctgcgtgagg ctgggctcct ggatgctgcg ggcgcgccca tcgccgacgc cttcgcccga
     2101 ctgctcgccg agaccgcgcc agtcacccgt caggggctgg agcgctgggt caccgctggc
     2161 gtgatcacgg agtggccatc acggccagcc gaccgcgacg agcttcttgc ctgggtgctc
     2221 gagcgcgcgg cggaaccggg cgagagtctc ggcgagcgcg aactcaccga gcggcttgcg
     2281 gccctcgggc acgacccggt cgcattgcgg cgggcactgg tcgacgcgga gctcgtgacc
     2341 cgcaccgttg acggctccac ataccggcga agctaggact cgtcggcgac accctcgccg
     2401 gcgacgctct cgccgcccac gctctcgcgc tcggcgacca cctcgcgggc ccgtctgcgc
     2461 gagacgcggt cgagaagcag ggccagcagc gcacccagca gcacgcccgc cggaatgccg
     2521 aacaggcaga agtagccgaa cagggcgatg aacccgacat tcgggtcgac cgggaactgc
     2581 gcggtcagca cgagcgtcac cagcgcaccg atcagaccgc ccaccacgag gaatgcgccg
     2641 atcttgggcg ctcggcggat cttcacggtg ctttccggtt cgctcacccc tccattgtct
     2701 cccacgcgag tgggaggtag ggcgcgaggt cagcccgggc ccccgaggcc gacactgcac
     2761 cggatgccac ggccagcgcc cacgtcgtgc ccccggttgc cagcgcgagc caggtcggcg
     2821 catccatctc gatgacattg ggaggcgtgc cgcgggtgtg gcgcggcccc tcgacggcct
     2881 gcaccgcgcc gaacggcggc acccgcactt cgacggtgtt gccctccgcg ttctccgcta
     2941 gcacctgcag caggtaccgg gtggcagtcg cgaggtcggg cttcgagccc gcgagcgcgg
     3001 ctgtcacg
//
LOCUS       DAOSOU010000242         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_32821_length_3003_cov_4.742877, whole genome shotgun sequence.
ACCESSION   DAOSOU010000242 DAOSOU010000000
VERSION     DAOSOU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32821_length_3003_cov_4.742877"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLW15_10745"
     CDS             complement(<1..772)
                     /locus_tag="PLW15_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT34912.1"
                     /translation="MVVELSQTDARRIAVRAQLLDAHRPDTLLDVVRQLTFVQLEPTA
                     PIAPTADLLLFSRLGDAYEPADLTAALEEEHSLFDLDLLVRPMEDLALFRAQMDAFPE
                     RETTRRWLDDNEEFRGDVLELLEADGPLTARGIPDTSIVAWPSSGWNNNRNVMMMLQC
                     LMLSGEVAVAGRSGRDRLWDLAERVYHSDIPTVPLEEALRIRDERRLRSLGVVRNRTP
                     DLPVETTRVGDAGVTATIEGLTGVWRLDPAALDRDFSPR"
     gene            864..1436
                     /locus_tag="PLW15_10750"
     CDS             864..1436
                     /locus_tag="PLW15_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HOT34913.1"
                     /translation="MNPSLHITGDTAADALLSDDPLALLIGMLLDQQVPMETAFAGPA
                     KLRDRLGRLDPAEIASYDADKMVDVFRISPSIHRFPGSMAGRVQALCEAVVNDWGGEA
                     SAIWTQGNPDGAEVLARLNALPGFGEQKAKIFLALLGKQYGFEGAGWREAAGDYGTAG
                     SWRSVADIVDAESLTKVREFKRAAKAAAKK"
     gene            1433..1936
                     /locus_tag="PLW15_10755"
     CDS             1433..1936
                     /locus_tag="PLW15_10755"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT34914.1"
                     /translation="MTTAREIVDALGLEPNQTCGYVRVTFGSPLQLPAGTLPHPYTAA
                     RPLGSALYFLVAPEAPVRLHRIANDQLYHYYAGDPLDLVLMLPDGSTAQHVVGGDVLA
                     GQVPQLLIPGGTFHTARVRGDWFLGGSTEWPGVVPSDVELGDPDALAERFPDVAEQIR
                     TYPVPTR"
     gene            1979..2659
                     /locus_tag="PLW15_10760"
     CDS             1979..2659
                     /locus_tag="PLW15_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HOT34915.1"
                     /translation="MRVGFAILRVAAAAAGVAAIVGQLITSLDFWGKNNVTNIPLVVT
                     NFFSFFTIQSNVAAVVVFLIGAALLVTRTGDDPQWFNTLRASVVTYMVVTGIVYNLLL
                     RNVPLPQGATLPWSNEILHVAVPILMLLDWIFTPGRMVVPWRKLWIIVIYPIVWVVYT
                     MIRGPLTPNEITGESYWYPYPFLNPDTSPNGYLSVSFYVVLIALVIVAVGAGIAWISR
                     RKPILPGA"
     gene            complement(2842..>3003)
                     /locus_tag="PLW15_10765"
     CDS             complement(2842..>3003)
                     /locus_tag="PLW15_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOT34916.1"
                     /translation="KRVAATVAEESYLDNSPPLPTPEETALLPRRDRGAGRPTKRERR
                     EIEKLLGRD"
BASE COUNT          447 a         1046 c          992 g          518 t
ORIGIN      
        1 tgcgcgggct gaagtcccgg tcgagagccg cggggtcgag ccgccacacg ccggtcaggc
       61 cctcgatcgt ggcggtgaca ccggcgtcgc cgacgcgcgt cgtctccacc ggcaggtcgg
      121 gcgtgcggtt gcgcacgacg cccagcgagc gcaggcgtcg ctcgtcgcgg atgcgcagtg
      181 cctcctcgag cggcaccgtc gggatgtcgg agtggtacac ccgctcggca aggtcccaca
      241 ggcgatcgcg cccgctgcga cccgccacgg cgacctcgcc cgacagcatc aggcactgca
      301 gcatcatcat gacgttgcgg ttgttgttcc agcccgacga cggccacgcc acgatgctgg
      361 tgtccgggat gccacgagcc gtcagcggcc cgtcggcctc cagtaactcc agcacgtcgc
      421 cgcggaactc ctcgttgtcg tcgagccagc ggcgcgtcgt ctcgcgctcc gggaacgcat
      481 ccatctgcgc ccgaaacaac gccaggtctt ccatcggccg cacgagcagg tccaggtcga
      541 agaggctgtg ctcctcttcg agagccgcgg tgaggtcggc cggttcgtag gcatccccga
      601 gccggctgaa gagcagcaga tcggcggtcg gcgcgatcgg ggcggtcggt tcgagctgca
      661 cgaaggtcag ctggcgcacg acgtcgagca gggtgtcggg gcggtgggcg tcgagcagtt
      721 gcgctcgcac ggctatgcgg cgggcatccg tctggctcag ctccacgacc acgacgcaga
      781 gcctagctcg ggtctacgac agccaggccg ccaggccacg tcagaccttg cctccaaggg
      841 ccaagtcaag cgacgatcgg aacatgaacc catcgctgca catcaccggc gacacggccg
      901 ccgatgcact tctgtcggat gacccgctcg ccctgctcat cggaatgctg ctcgaccagc
      961 aggtgccgat ggagacggcc ttcgccgggc ctgcgaaact ccgcgaccgt ctggggcgac
     1021 tcgaccccgc cgagatcgcc tcctacgacg ccgacaagat ggtggacgtg ttccgcatct
     1081 cgccctcgat ccaccgtttt cccggttcga tggccgggcg ggtgcaggcg ctgtgcgagg
     1141 ccgtggtcaa cgactggggc ggcgaggcat ccgccatctg gacccagggc aaccccgacg
     1201 gagccgaggt gcttgcgcga ctcaacgccc tgcccgggtt cggcgagcag aaggcgaaga
     1261 tcttcctcgc gctgctgggc aagcagtacg ggttcgaggg tgccggctgg cgcgaggccg
     1321 cgggcgacta cggcacggcg ggctcgtggc gctcggtggc cgacatcgtc gacgccgaat
     1381 cgctgaccaa ggtgcgcgag ttcaagcggg cggcgaaggc ggcagccaag aagtgaccac
     1441 cgcacgcgag atcgtggatg cgctgggtct cgaacccaac cagacctgcg gttacgtgcg
     1501 ggtcaccttc ggtagcccgc tgcagctgcc cgccgggacc ctgccgcacc cctacaccgc
     1561 ggctcgcccg ctgggctcgg ccctctactt cctcgtggcc ccggaggctc ccgtgcgcct
     1621 acaccgcatc gcgaacgacc agctgtacca ctactacgcc ggcgacccgc tcgacctggt
     1681 gctcatgctg cctgacggca gcacagcgca gcacgtcgtg ggcggcgatg tgctggcggg
     1741 ccaggtgccg cagttgctca tccccggcgg caccttccac acggcgcggg tgcgcgggga
     1801 ctggttcctc ggcggcagca cggagtggcc cggtgtcgtg ccctccgacg tggaactcgg
     1861 cgaccccgac gcgctggcgg aacggttccc cgacgtcgcc gagcagatcc gcacctatcc
     1921 ggtgcccacc cgatagcatt ttcgtgcgca cgcgacggcc gctgcgctgg agggaaccat
     1981 gcgcgtcgga tttgcgatcc tgagagtggc ggccgctgcc gccggagttg cagcgattgt
     2041 cggtcagctg atcacgagtc tcgacttctg gggcaagaac aacgtcacga acatccctct
     2101 ggtcgtcacg aacttcttca gcttcttcac aatccagtcg aacgtcgcgg cagtcgtcgt
     2161 gttcctgatc ggtgctgcgc ttctggtcac gcgcacgggc gacgacccgc agtggttcaa
     2221 cacgcttcgc gccagcgtcg tgacctacat ggtcgtcacc ggcatcgtct acaacctgct
     2281 gctgcgcaat gtgcccctgc cgcagggcgc gaccctgccg tggtcgaacg agatcctgca
     2341 cgttgccgtg ccgattctca tgctcctcga ctggatcttc accccgggtc gaatggtggt
     2401 gccgtggcgc aagctctgga tcatcgtgat ctacccgatc gtctgggtgg tgtacacgat
     2461 gatccgcggc ccgctcaccc ccaacgagat cacgggcgag tcgtactggt acccgtaccc
     2521 gttcctcaac cccgacacct cgccgaacgg ctacctgtcg gtgagcttct acgtggtgct
     2581 catcgcactc gtgatcgtcg ccgtcggcgc cggcatcgcg tggatcagcc gcaggaagcc
     2641 catcctgccc ggggcttagt ccttgcgctt ggacgcggcc ttgtccttct tgtcctgacg
     2701 cttctccttg aggctcttcg aggcggcggt cttcgtcgtg gtcttcttgg gtgacttgtc
     2761 ggccatggtc agctcctcct gagcgaatgc tcacgagcgg gatgctcgta ccccgacctt
     2821 acgccagggt gactggcggt gtcagtcgcg accgagcagc ttctcgatct cgcggcgctc
     2881 ccgcttggtc ggtcggcccg cacctctgtc gcgccggggg agcagcgccg tctcctcagg
     2941 ggtgggcagg ggcggtgagt tgtcgaggta gctctcctcc gcaaccgtgg ctgcaacccg
     3001 ctt
//
LOCUS       DAOSOU010000243         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_33240_length_2973_cov_5.314942, whole genome shotgun sequence.
ACCESSION   DAOSOU010000243 DAOSOU010000000
VERSION     DAOSOU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33240_length_2973_cov_5.314942"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1751
                     /gene="kdpA"
                     /locus_tag="PLW15_10770"
     CDS             60..1751
                     /gene="kdpA"
                     /locus_tag="PLW15_10770"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOT34917.1"
                     /translation="MDAATAAPTIWLFLAQLGTVVLVLALLYRPLGDYIAATYTSTRD
                     LRAEKGFYRLIGADSASQQSWPAYLRSVLAFSAVGVLAVYALQRLQAVLPYSLGLPPT
                     SEPLAFNTAISFVTNTNWQSYSPELTLGYTVQLVGLAVQNFLSAAVGIAVAVALIRGF
                     AYRSSGTIGNFWVDLTRGTLRILLPLSIVGAVVLLAGGVIQNFAGFQEVTTLTGGTAV
                     VPGGPVASQEAIKLLGTNGGGFFNANSAHPFENPTPWTSIIEVLMFLVIPFAMPRAFG
                     RMVGDNRQGVAIVGTMAVLFVVSLSLMTWAELAGSGTAPQLAGGAMEGKETRFGILGS
                     TLYATSTTLTSTGSVNSMHDSFTSLGGGVALVNMMLGEVAPGGVGGGLYGILVVAIIA
                     VFIAGLMVGRTPEYLGKRLGAREIKLASLYILTTPTLVLVGTALSFAIPAVREDVEGT
                     SIWNPGLHGFSEVLYAFTSAANNNGSAFAGLTANTPWFNTALGVAMFLGRFLPIVFVL
                     ALAGSLAAQDKIPVTPGTLPTHRPQFVGLLIGTVLLVSALTYFPVLALGPLAEGL"
     gene            1760..>2973
                     /locus_tag="PLW15_10775"
     CDS             1760..>2973
                     /locus_tag="PLW15_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT34918.1"
                     /translation="MNTYLTALPGAFRKLDPRLMWRNPVMFVVEVGAVLTTALAVAQP
                     LMGVEDIAPGFTAGITAWLWVTVIFANLAESVAEGRGKAQAASLRQTRTSTTAHVVAA
                     SAIEQMSPTAPLETLDHRDVSSADLVLGDHVVVSAGEVIPGDGDIVWGIASVDESAIT
                     GESAPVIREHGGDRSAVTGGTRVLSDRIVVAITSKPGETFVDRMIRLVEGASRQKTPN
                     EIALNILLATLSIVFVVVVLTLNPIASYAGAAVSITVLVALLVTLIPTTIGALLSAIG
                     IAGMDRLVQRNVLAMSGRAVEAAGDVTTLLLDKTGTITYGNRQASEFLPVGGASLRAL
                     TEAASLSSLSDPTPEGSSVVALAAEKGFEAHAVTDAVIVPFTAQTRMSGLDLPDGSQV
                     RKGAGSMIAQWVG"
BASE COUNT          452 a         1039 c          938 g          544 t
ORIGIN      
        1 ctgggactcg ctgcgatcgg ctacctggtc gtcgctctcg tgaagccgga gcgtttctga
       61 tggatgccgc aacagcagct cccaccatct ggttgttcct cgcccagctc ggcaccgtgg
      121 tgctggtgct cgcactgctc taccgccccc tcggggacta catcgctgcg acctatacga
      181 gcacccgtga cctgcgggcg gagaagggct tctaccgact gatcggtgcc gactcggcct
      241 cccagcagag ttggccggca taccttcggt cggtgcttgc gttctccgcc gtcggcgtgc
      301 tcgcggtcta tgcgctgcaa cgactccagg cggtgcttcc ctactcgctg ggactgccgc
      361 cgaccagcga gcccctcgcc ttcaatacgg ccatctcatt cgtcacgaac acgaactggc
      421 agagctactc tcccgagttg actctcggct acaccgtgca gctggtcggc ttggccgtgc
      481 agaacttcct gtcggcggcg gtcgggatcg ctgtggcggt cgccctcatc cgtggcttcg
      541 cgtatcgctc ctcgggcacc atcggcaact tctgggtgga cctcacccgg ggcacgctac
      601 gcatcttgct cccgctgtcg atcgtgggcg cggtcgtgct gctcgccggg ggcgtcatcc
      661 agaacttcgc gggattccag gaagtcacca cgctcaccgg cggtaccgca gtggttccgg
      721 gcggacccgt cgcctcacag gaggcgatca agcttctcgg caccaacggt ggcggattct
      781 tcaacgccaa ctcggcgcat ccgttcgaga accccacgcc ctggacgagc atcatcgagg
      841 tgctgatgtt cttggtcatc cccttcgcca tgcctcgcgc gttcggccgc atggtcggcg
      901 acaaccgaca gggcgtcgcc atcgtgggaa cgatggccgt gctgttcgtc gtgtcgctgt
      961 cgctcatgac ctgggccgag ctggcggggt cgggcacggc accacagctg gcgggcggcg
     1021 ccatggaggg caaggagacg cggttcggga ttctcggctc gaccctgtac gcgacatcca
     1081 cgaccctgac gtcgaccgga tcggtcaact cgatgcacga cagcttcacg agtctcggtg
     1141 gtggcgtcgc gctggtgaac atgatgctcg gcgaggtcgc accggggggt gtgggcggcg
     1201 gcctgtacgg catcctcgtg gtcgccatca tcgcggtctt catcgcggga ctcatggtcg
     1261 gtcgcacccc ggagtacctc ggcaagcgac tcggcgcgcg agagatcaag ctggcgagcc
     1321 tgtacatcct gacgacgccg accctcgtgc ttgtgggcac tgcgctgagc ttcgcgatcc
     1381 ccgcggttcg cgaggacgtc gagggcacct cgatctggaa tcccggactg cacggcttca
     1441 gcgaggtgct gtacgccttc acctcggccg ccaacaacaa cggttcggcc ttcgcgggtc
     1501 tcaccgccaa caccccgtgg ttcaacacag cgctgggtgt ggcgatgttc ctcggccggt
     1561 tcctgccgat cgtcttcgtg ctcgcgctgg ccggttcgct ggccgctcag gacaagatcc
     1621 ccgtgacccc cggcaccctg cccacccatc gaccccagtt cgtcggcctg ctcatcggca
     1681 ccgtcctgct ggtgtcggct ctcacctact tccccgttct cgcgctgggt cccctggcgg
     1741 aaggtctgta acgacaacca tgaacacgta tctcactgcg ctccccggag cgttccgcaa
     1801 gctcgaccct cgcctcatgt ggcgcaaccc cgtgatgttc gtcgtcgagg tgggcgccgt
     1861 gctcaccacg gcgctggcag tcgcccagcc tctgatgggt gtcgaagaca tcgcacccgg
     1921 cttcaccgcc ggaatcaccg cgtggctctg ggtcaccgtg atcttcgcga acctcgcgga
     1981 gtcggtggcg gagggtcggg gaaaggccca ggccgccagc ctccgccaga cccgcacctc
     2041 gacaacagct cacgtcgtgg ctgcatccgc aatcgagcag atgagcccga cagctccact
     2101 cgagaccctc gatcaccgtg atgtctcctc ggcagacctg gtgctcggcg atcacgtcgt
     2161 cgtgtcggcc ggcgaggtca ttcccggcga cggcgacatc gtgtggggaa tcgcctccgt
     2221 cgatgagtcg gccatcacag gcgaatcggc cccggtcatc cgggagcacg gtggagaccg
     2281 cagtgccgtc accggtggca ccagggtgct cagcgaccgc atcgtggtcg ccatcacctc
     2341 caagccgggc gagactttcg tcgaccggat gatccggctc gtcgagggcg cctcgcgcca
     2401 gaagacgccc aacgagatcg ccctcaacat cctgctcgcc actctgtcga tcgttttcgt
     2461 ggtggtagtg ctcactctca acccgatcgc gagctacgcg ggagccgcgg tgagcatcac
     2521 ggtgctcgtc gccctgctcg tcacgctcat ccccaccacg atcggtgccc ttctgagcgc
     2581 catcggaatc gccggaatgg accgactcgt acagcgcaac gtgctcgcga tgtcggggcg
     2641 cgccgtcgag gcggcgggag acgtgacgac cctgctgctc gacaagaccg gaaccatcac
     2701 ctacggcaat cgtcaggcga gcgagttcct gccggtcggt ggcgcgtcac ttcgcgcact
     2761 caccgaggcc gcgagcctgt catctctcag cgacccgact cccgagggca gctcggtcgt
     2821 cgctctcgcg gcagagaagg gcttcgaagc gcacgccgtc acggatgcag tgatcgtgcc
     2881 gttcaccgcc cagacccgca tgagcggact cgacctgccg gacggcagcc aggtgcgcaa
     2941 gggggcggga tcgatgatcg cccagtgggt cgg
//
LOCUS       DAOSOU010000244         2960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_33446_length_2960_cov_5.926678, whole genome shotgun sequence.
ACCESSION   DAOSOU010000244 DAOSOU010000000
VERSION     DAOSOU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33446_length_2960_cov_5.926678"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..967
                     /locus_tag="PLW15_10780"
     CDS             14..967
                     /locus_tag="PLW15_10780"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOT34919.1"
                     /translation="MSVIEQSKLAIIGAGAVGTSLAYAAMIRESAREIALYDIRTEKV
                     AAEVLDLAHGSQFTGVSRIQGSDDLDVVQGSNVVVITAGAKQHPGQSRLDLAADNVAI
                     LRDLMPKLLDRAPEAVYILVSNPCDVLAVAAQRITGLPTSQIFASGTVLDSSRLRWLL
                     SERVGVATSSVHAMIVGEHGDSEFPLWSQARIGPVPILEWSLENGHPLDAAELATIAE
                     DVKTAAYRVIAGKGATNFAIGLSGARIVEAILRDEGRILPVSSVLDDYRGISGVALSV
                     PSVVDARGVAQVIDVPYSVDEMRMLEASAATLRSSLSSLGF"
     gene            complement(1009..1593)
                     /gene="rdgB"
                     /locus_tag="PLW15_10785"
     CDS             complement(1009..1593)
                     /gene="rdgB"
                     /locus_tag="PLW15_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOT34920.1"
                     /translation="MIRVVLATHNAHKVTELRTILGPALAGIDLVAYDGPEPVEDGET
                     FEENALIKARAASAHTGLPALADDSGICVDALGGAPGIHSARYAGTRDDADNVALLLA
                     TLGAEPNRAAQFVCAAALVIGDREFVERGTWPGTVLFEAAGPNGFGYDPVFKPAGLSV
                     SSAELRPDEKNAISHRQRAFSALIGATREILLDS"
     gene            complement(1590..2312)
                     /gene="rph"
                     /locus_tag="PLW15_10790"
     CDS             complement(1590..2312)
                     /gene="rph"
                     /locus_tag="PLW15_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT34921.1"
                     /translation="MTRADGRENTQLRPVTIERGWSEQAEGSALISFGRTKVLCTASF
                     TNGVPRWLTGKGKGWVTAEYGMLPRSTNDRMDRESVKGKVGGRTHEISRLIGRSLRAV
                     IDTKALGENTIVIDCDVLQADGGTRTAAITGAYVALADAIEWGRDHNFIGKNAKALHD
                     SVAAVSVGIIDGEPMLDLAYTEDSRAETDMNVVVTGRGLFVEVQGTAEGAPFDRRELD
                     SLLDLALGGAADLTALQVAALA"
     gene            complement(2339..>2960)
                     /locus_tag="PLW15_10795"
     CDS             complement(2339..>2960)
                     /locus_tag="PLW15_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34922.1"
                     /translation="GLAVLDGAEWIVHHDEPIVRASALPVRGRHNAVNAALALAAVEV
                     SGVTIDPELALAALKSFAPLEHRLEPIADPSGLTFVDDSLSTSPFAAIEALKAFGSDG
                     VVLIVGGQDRGVDYSPLAEHLAQHPVTAVVGLPPGGPRILDVIASTGIPSETAEDMLD
                     AVTRARRLAAPGGTVLLSPAAPSYGIYRDFAHRAEVFRAAIVATRP"
BASE COUNT          445 a         1005 c         1038 g          472 t
ORIGIN      
        1 ccatgctcga tgcatgagcg ttatcgaaca gtcgaagctg gccatcatcg gcgctggagc
       61 ggtgggcacc tcgctcgcct acgcggcaat gatccgcgag tcggcgcgcg agatcgcact
      121 gtacgacatc cgcaccgaga aggtggcagc cgaggtgctc gacctcgcgc acgggtcgca
      181 gttcacgggg gtgtcgcgca tccagggctc cgatgatctg gatgtcgtgc agggctcgaa
      241 cgtcgtcgtc atcaccgcgg gggccaagca gcaccccggt cagtctcgac tcgacctggc
      301 ggccgacaac gtcgccatcc tgcgcgatct catgccgaag ctgctcgacc gcgcacccga
      361 ggccgtctac atcctcgtgt ccaacccctg cgacgtgctc gccgtggccg cccagcgcat
      421 caccggcctg ccgacatcac agatcttcgc ctccggcacg gtgctcgatt cgtcgcggct
      481 gcgatggctg ctctccgagc gggtcggggt cgcgacatcc agtgtgcacg ccatgatcgt
      541 cggggagcac ggcgactccg aatttccgct gtggtcgcag gcgaggatcg ggccggtacc
      601 gatcttggag tggtcactcg aaaacgggca tccgctcgac gcggccgagc tggccaccat
      661 cgccgaggac gtcaagaccg cggcctaccg ggtgatcgcc ggcaagggcg ccaccaactt
      721 cgccatcgga ctctcgggcg cccgcatcgt cgaggccatt ctgcgcgacg agggcaggat
      781 ccttccggtc agttccgtgc tcgacgacta ccggggcatc tccggtgtcg cgctgtcggt
      841 gccgagcgtg gtcgacgcac gcggggtcgc ccaagtgatc gacgtgccgt attcggttga
      901 cgagatgcgg atgctcgagg cctccgccgc gactctgcga tcgtcactgt cgagcctggg
      961 attctgagct cggcttctga ccgcagccgg gtcctctgac cgccgaagtc aggagtcgag
     1021 gaggatctcg cgggtcgcac cgatcagcgc gctgaacgcc cgctgacggt ggcttatcgc
     1081 gttcttctcg tcgggccgca gctccgcgga actcacgctg agcccggccg gcttgaacac
     1141 cgggtcgtac ccgaaaccgt tcggaccagc ggcctcgaac agcacggtgc cgggccaggt
     1201 gccgcgctcg acgaactcgc ggtcgccgat cacgagggcc gcggcgcaga cgaactgcgc
     1261 ggcccggttg ggttcggccc cgagagtggc gagcagcagc gcgacattgt cggcgtcatc
     1321 tcgggtgccg gcatagcgag ccgagtggat gcccggggct cccccgagcg cgtcgacgca
     1381 gatgcccgag tcgtcggcga gcgccggaag tccggtatga gcggatgccg cgcgcgcctt
     1441 gatcagcgca ttctcctcga acgtctcgcc atcctcgacc ggctcgggcc cgtcatacgc
     1501 gaccaggtcg atacccgcca gcgccggacc gaggatcgtg cgcagctcgg tgaccttgtg
     1561 cgcgttgtgg gtggcgagta cgaccctgat catgccagcg ccgcgacctg cagcgcagtg
     1621 aggtcggccg cgcccccgag ggcgagatcg agcaggctgt cgagttcgcg acggtcgaag
     1681 ggtgcgccct cggcggtgcc ctgcacctcg acgaacaggc cacgaccggt gacgacgacg
     1741 ttcatgtcgg tctcggcgcg cgagtcctcg gtgtaggcca ggtcgagcat cggctcgccg
     1801 tcgatgatgc cgacgctgac cgcggcgact gagtcgtgca gggccttggc gttcttgccg
     1861 atgaagttgt ggtcgcgacc ccactcgatc gcgtcggcga gcgcgacata ggctcccgtg
     1921 atcgcggcgg tgcgcgtgcc gccgtcggcc tgcagtacgt cgcagtcgat cacgatggtg
     1981 ttctcgccga gcgccttggt gtcgatcacg gcacgcaacg aacgaccaat caggcgcgag
     2041 atctcgtgcg tgcggccgcc gaccttgccc ttgaccgact cgcgatccat gcggtcgttc
     2101 gtggagcgcg gcagcatgcc gtactccgcg gtgacccagc ccttgccctt gccggtcagc
     2161 cagcgcggaa ccccgttggt gaagctcgcc gtgcacagca ccttggtacg cccgaaggag
     2221 atgagcgccg acccttcggc ctgttcgctc cagccgcgct cgatggtcac ggggcgcagc
     2281 tgggtgttct cacggccgtc ggctcgggtc atggtcttct ctcgtggttg gggcggggtc
     2341 aggggcgggt ggcgacgatc gccgcgcgga acacctcggc gcggtgcgcg aagtcccggt
     2401 agataccgta gctgggcgcg gcgggcgaga gcagcacggt gccaccgggg gcggccagac
     2461 gacgggcgcg ggtgacagcg tcgagcatgt cctcggccgt ttcgctcggg atgcccgtgc
     2521 tcgcgatcac gtcgaggatg cgcggaccac cgggcggcag gcccaccacc gcggtgacgg
     2581 gatgctgcgc cagatgctcg gcgaggggcg agtagtccac cccccgatcc tgcccgccca
     2641 cgatcagcac gacaccgtcg gagccgaagg ccttcagcgc ctcgatcgcc gcgaacgggc
     2701 tcgttgagag ggagtcatcc acgaacgtca gaccggaggg gtcagcgatc ggctcgaggc
     2761 gatgctcgag cggggcgaag ctcttcaggg cagcgagcgc gagctcgggg tcgatggtca
     2821 cgccgctgac ctccacggcc gcgagtgcga gagccgcgtt gaccgcattg tgtcgccccc
     2881 gcaccggcag cgcggatgcg cgcacgatgg gctcgtcgtg gtgcacgatc cactcggcac
     2941 cgtcgagcac ggcgaggccc
//
LOCUS       DAOSOU010000245         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_33484_length_2957_cov_7.321502, whole genome shotgun sequence.
ACCESSION   DAOSOU010000245 DAOSOU010000000
VERSION     DAOSOU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33484_length_2957_cov_7.321502"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PLW15_10800"
     CDS             <1..167
                     /locus_tag="PLW15_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOT34923.1"
                     /translation="YFQPDDLSVPEEYRGIGVRIEDNILVTETGAENLSVGIPRTADD
                     VEAWLSRAGA"
     gene            complement(241..738)
                     /locus_tag="PLW15_10805"
     CDS             complement(241..738)
                     /locus_tag="PLW15_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34924.1"
                     /translation="MSNPSTLGRRGVPAPSLPKGDVLGTYDTYPEASSVVDRLSKAEF
                     DVKQLSIIGNDLKTVERVTGKLTYGRAALAGAASGAWLGLFFGLLLTIFGPVSTQSLT
                     LVGAALIIGAGFGMIFGIVTYAINRRRRDFTSTHQVIASNYQIIIDPELTAKAQQTLA
                     EHPAE"
     gene            775..2070
                     /locus_tag="PLW15_10810"
     CDS             775..2070
                     /locus_tag="PLW15_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT34925.1"
                     /translation="MSTTRVFAARLVGCSVFDPNGDRVGKVRDVLVVYRTNGGPRVVG
                     MVLEVPGKRRVFLSIGRVTSIGSSQIIATGLINLRRFEQRGGEVRVIAEMLGRKVRLR
                     DGSGHANIEDVAIEEVGLGDWEISQVFLRRPKTGGSPFSKGATVFANWNDLIEDAPAG
                     ESQSATQLVASYADMLPADLASAMLDLPEQRMLEVAEELSDDRLADVLEEMPEDEQVD
                     ILNQLDDDRAADVLDQMQPDDAADLIAHLSDERGEALLELMEPEEAEDVRFLLSYAPD
                     TAGGLMTTEPIIVSADATVAEGLALIRRHELAPALGAAICVTLPPYEPPTGRFLGMVH
                     FQRMLRYPPNERLGTLLDQSLEPVQANTSAAEVSRILASYNLVSVPVVDENHRLVGVV
                     TIDDVLDYLLPDDWRSTTDDQPSKPQQPVKGRRTPSGTR"
     gene            2057..2557
                     /locus_tag="PLW15_10815"
     CDS             2057..2557
                     /locus_tag="PLW15_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOT34926.1"
                     /translation="MARANTRNDTRLDSPKGLRTNFPLFQSRDRMGRFSEAFARAMGT
                     PWFLVGMTVVVVVWLTYNTLAPEEAQFDPRLTNFTLLTLILSLQASYAAPMILLAQNR
                     QDDRDRVQIEQDRQRAERNLNDTEYLAREVVSLRLAVKDLASKDFIRAELRALLEELE
                     KDKPVE"
     gene            2550..>2957
                     /locus_tag="PLW15_10820"
     CDS             2550..>2957
                     /locus_tag="PLW15_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOT34927.1"
                     /translation="MSSDRRSRIETSGDPGFDTTDFAGLLNQRIRESLSRVIDPELRR
                     PVTELDMIGGVSVDEAGAASVDLKLTIVGCPAADTIERDVRAAVAGTAGVTAVALSVG
                     VMSREEREALTDKLRGGRPKALQFGPDSLTRIYA"
BASE COUNT          500 a          998 c          980 g          479 t
ORIGIN      
        1 tctacttcca gcccgacgac ctgagcgttc ccgaggagta ccgcggcatc ggcgtgcgca
       61 tcgaggacaa cattctcgtc accgagaccg gcgccgagaa cctgtctgtc ggcatcccgc
      121 gcaccgccga cgacgtggag gcgtggctct cgcgcgctgg agcttagagc gaaggccatt
      181 tctggccttc gccgcgacgc cagcgctgag cgccgtctcg gcgctcaggt tctactcagg
      241 ctactcggcc ggatgctccg cgagggtctg ctgcgccttg gccgtcagct ccgggtcgat
      301 gatgatctgg tagttgctcg cgatcacctg gtgggtggac gtgaagtcgc gacggcgacg
      361 gttgatcgcg taggtgacga tgccgaagat catgccgaac ccagcaccga tgatgagggc
      421 ggcgcccaca agggtgaggg attgggtgga gaccggcccg aagatggtca gcaggaggcc
      481 gaagaacagg ccgagccagg caccgctcgc ggcaccggcg agggcagcac gaccgtaggt
      541 gagcttgccc gtcacgcgct cgaccgtctt gaggtcattg ccgatgatcg acagctgctt
      601 gacgtcgaac tccgccttgc tcagccgatc gacgaccgat gacgcctcgg ggtacgtgtc
      661 gtaggtgccg agcacatcgc ccttgggcag gctcggggcg ggcacgcctc tgcggccgag
      721 tgtggaaggg ttgctcacga acccattgtt ccacgcgggg gtagggtttt acccgtgagc
      781 acgaccagag tcttcgccgc ccggttggtc gggtgctccg ttttcgaccc caacggcgac
      841 cgggtcggca aggttcgcga cgtgctcgtg gtgtaccgca cgaacggcgg gccgcgcgtg
      901 gtgggaatgg ttcttgaggt tccgggtaag cgccgggtgt tcctgtcgat cgggcgggtc
      961 accagcatcg gttcgagtca gatcatcgcc acgggcctca tcaacctgcg ccgtttcgaa
     1021 cagcgcggcg gcgaggtgcg agtcatcgcc gaaatgctgg gccgcaaggt gcgcctgcgc
     1081 gacggctccg gccacgccaa catcgaggat gtcgcgatcg aagaagtcgg tctcggcgac
     1141 tgggagatca gtcaggtgtt cctgcgccgc cccaagaccg gtggctcgcc cttctccaag
     1201 ggcgcgacgg tcttcgccaa ctggaacgac ctgatcgagg atgccccggc cggcgaatcg
     1261 cagtccgcca cccagctcgt ggccagctac gccgacatgc ttcccgccga cctggccagc
     1321 gccatgctcg acctgcctga gcagcgcatg ctcgaggtcg ccgaggagct gagcgatgac
     1381 cgtctcgccg acgtgctcga ggagatgccc gaagacgagc aggtcgacat cctgaaccag
     1441 ctcgatgacg accgggcggc cgacgtgctt gaccagatgc agccggatga cgcggcagac
     1501 ctcatcgcgc acctgagcga cgagcgcggc gaggcgctcc tggagctcat ggagcccgag
     1561 gaggcggagg acgtgcgctt cctgctgtcc tacgccccgg acaccgcggg cggtctcatg
     1621 accaccgaac cgatcatcgt gtcggcggat gccacggtcg ccgagggtct cgcgctgatc
     1681 cgccgccacg agctcgcgcc cgccctcggc gccgccatct gcgtgaccct cccgccctac
     1741 gagccaccga ccggccggtt cctcggcatg gtgcacttcc agcgcatgct gcgctacccg
     1801 cccaacgagc gcctcggcac gctgctcgac cagagcttgg agcccgtaca ggccaacacg
     1861 agcgcggccg aagtctcgcg catccttgcc agctacaacc tcgtctcggt tcccgtcgtt
     1921 gacgagaacc accgcctggt cggggtggtg accattgacg atgtcctcga ctatctgctg
     1981 ccggatgact ggcgaagtac caccgacgat cagcccagca aaccgcagca gcctgtgaag
     2041 ggaaggagga cacccagtgg cacgcgctaa cacccgtaac gacacgcgac tcgactcacc
     2101 gaagggtctg cgcaccaact tcccgctgtt ccagagccgc gaccgcatgg gccgattctc
     2161 cgaggcgttc gcccgcgcga tgggtacgcc gtggttcctc gtcggcatga ccgtggtcgt
     2221 ggtcgtgtgg ctgacttaca acacgctcgc gcccgaggag gcgcagttcg acccgcgcct
     2281 gaccaacttc acgctgctga cgctgatcct ctcgttgcag gcctcgtacg cggctccgat
     2341 gatcctgctc gcgcagaacc gtcaggacga ccgcgaccgc gtgcagatcg aacaggaccg
     2401 tcagcgcgcc gagcgcaacc tcaacgacac cgagtacctc gcccgcgagg tggtctcgct
     2461 gcgcctggcc gtgaaagatc tcgccagcaa agacttcatc cgtgccgagc tgagggccct
     2521 gctggaggag cttgagaagg acaagccggt tgagtagcga ccgtaggtcg cgtatcgaaa
     2581 ccagtggcga tcccggtttc gatacgaccg acttcgccgg tctactcaac cagcgcatcc
     2641 gcgagtccct ctcccgcgtg atcgaccccg agctgcggcg gccggtcacc gagctggaca
     2701 tgatcggtgg ggtgtccgtc gacgaggcgg gcgcggcatc cgtcgacctg aagttgacga
     2761 tcgtcggatg cccggccgcc gacacgatcg aacgtgacgt gcgcgcggcc gtcgccggca
     2821 cggccggggt cactgcggta gcgctcagcg tcggtgtgat gtcgcgcgag gaacgtgagg
     2881 cgctcaccga caagctgcgg ggcggccgtc cgaaggcgct ccagttcggc ccggactccc
     2941 tgacccgcat ctacgcc
//
LOCUS       DAOSOU010000246         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_33560_length_2951_cov_6.397445, whole genome shotgun sequence.
ACCESSION   DAOSOU010000246 DAOSOU010000000
VERSION     DAOSOU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33560_length_2951_cov_6.397445"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1206
                     /locus_tag="PLW15_10825"
     CDS             214..1206
                     /locus_tag="PLW15_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34928.1"
                     /translation="MTNWPARVFLVHELPALDVHPRSVSRAIERGELIRVKRGAYCPR
                     AVWDAATDPERHRIRVSAAAAAHPGVVFSHWSAAVLHGLPLIGPWPSTVHVAADRASG
                     GRSEVGLTRHCRGLRAEEIVDLGTLRATAVERTLADLAVVQPFRFATSPADYALRAAL
                     TSRDALMAHLEQPFRGRSRALQIAEFADGRAETPGESLSRATMHILGFPAPLLQERHV
                     SGRHVEFTDFEWPDYGVIGEFDGLGKYLREELRGNRSVAQVVIDEKQREDRLRRSSGS
                     RFARWGWDDALRMTPLRDILMESGLPIVRAGLTDSRGRYGGPDRPYRTRERGLA"
     gene            1225..1295
                     /locus_tag="PLW15_10830"
     tRNA            1225..1295
                     /locus_tag="PLW15_10830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1257..1259,aa:Gly,seq:ccc)
     gene            complement(1307..2704)
                     /locus_tag="PLW15_10835"
     CDS             complement(1307..2704)
                     /locus_tag="PLW15_10835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT34929.1"
                     /translation="MHTTAFERDLPAQEVIARSLASSVARPFWTDDAPPPPHESLTGS
                     RSADLVIVGAGYTGLWTAVRAKERNPGRSVVVVEAKRVGWAASGRNGGFVEASLTHGE
                     ENGQRRWPEELHELDRLGHENLDAIEATVARYSMQCDFERTGTLTVAVEPHQIEWLDA
                     DGRMDAAAAQAEVNSPTYLAAHWDRRSTALVHPARLANELARVAQELGVEIYESSPVR
                     RLDTGSDGVDVVTDGGSIRAKQVALATNVFPSLLKRNRLMTVPVYDYVLMTRPLTDGE
                     LASIGWTNRQGLADLANQFHYYRLSADNRILFGGYDAVYYAGGRVRSRYEERQQSFTR
                     LASHFFTTFPQLADVEFTHRWAGAIDTSTRFCAFFGTARGGQVSYAAGFTGLGVGATR
                     FAGDVMLDLLSGESTERTTLRMVRERPLPFPPEPAASIGINLTRWSLNRADHNEGRRN
                     LLLKTLDALGLGFDS"
     gene            2770..>2951
                     /locus_tag="PLW15_10840"
     CDS             2770..>2951
                     /locus_tag="PLW15_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HOT34930.1"
                     /translation="MSDAPLDLTALEVPTEPVAASRIVAGSPRDGATELTLFEGCEIG
                     VWEMTPGTATDVEVDEV"
BASE COUNT          465 a          974 c         1021 g          491 t
ORIGIN      
        1 gaccggatgt cgcgatcact gagaagcatg cggcaagact agccaagcca gcgcccgtgc
       61 gggcgctacg gactcgcgcg gtcgatacgg cgggcccgat cgtccgtacc gtacgcgcga
      121 cgtaccactc ctcccgatat tgacgggggc gcctggactc accggggcgg cggccaacgc
      181 gctcacggat gggggacgct ccatgatgcg aacgtgacca actggcctgc gcgggtgttt
      241 ctggtccacg agctacctgc gttggacgta cacccacgct ccgtttcccg agctatcgag
      301 cgtggcgagt tgattcgggt gaaacgcgga gcgtactgtc cgcgtgccgt gtgggatgct
      361 gccaccgatc cggaacgcca ccggattcgg gtgagtgcgg ccgctgccgc gcatcccggc
      421 gtcgtcttct cgcattggtc ggccgccgtt ctgcatggcc tgccactgat cggaccgtgg
      481 ccgtcgacag ttcatgttgc tgccgaccgc gcgagcggcg gccgctcgga ggtggggttg
      541 acccggcact gccgaggcct tcgcgccgag gagatcgtcg accttggcac gctgcgagca
      601 acggcggtcg agcgaaccct cgccgacctc gccgtggttc agccgttccg cttcgccaca
      661 tcccctgctg actacgcgct ccgcgccgcg ctgacatcgc gcgatgcgct catggcccat
      721 ctcgagcaac ccttccgtgg tcgatctcgc gcgctgcaga ttgcagagtt cgccgatggt
      781 cgagctgaga cgccaggcga gtccctcagc cgggcaacca tgcacatcct ggggtttcca
      841 gccccgctcc tgcaggaacg gcacgtctcc ggcaggcacg tcgagttcac agacttcgaa
      901 tggccagact acggggtgat cggggagttc gacggtctag gcaagtacct gcgagaggag
      961 ctcaggggga accggagcgt cgcgcaggtc gtgatcgacg agaagcagcg cgaggaccgg
     1021 ctgcgccgtt cgtccggcag tagattcgcg cggtgggggt gggacgacgc actgcggatg
     1081 acgccgctcc gcgacatcct tatggaatcg ggcctgccaa tcgttcgcgc gggccttacg
     1141 gactcgcgcg gtcgatacgg cgggcccgat cgtccgtacc gtacgcgcga gcgcggtcta
     1201 gcctagaatt ggcgctgcac gttcgcggat gtagttcaat ggcagaactt cagcttccca
     1261 agctgatagc gcgggttcga ttcccgtcat ccgctcagag agtcggctac gagtcgaacc
     1321 ccagccccag cgcgtcgagc gtcttcagca gcaggttgcg gcgcccctcg ttgtggtcgg
     1381 cgcggttgag tgaccagcga gtgagattga tgccgatgga tgccgcgggc tcgggcggga
     1441 acggaagcgg gcgttcgcgc accatccgta gggtcgtgcg ctcagtggac tcaccggaga
     1501 gcaggtcgag catgacgtcg cccgcgaagc gggtcgcgcc gacgccgagg ccggtgaagc
     1561 cggcggcgta ggacacctgt ccgccgcggg ccgttccgaa gaaggcgcag aaccgcgtgc
     1621 tggtgtcgat cgcccccgcc cagcggtggg tgaactcgac atcggcgagt tgcgggaagg
     1681 tcgtgaagaa gtgactcgcc agcctggtga agctctgctg acgctcctcg taccgcgagc
     1741 gcaccctgcc gccggcgtag tagacggcgt cgtatccgcc gaacaggatc cggttgtcag
     1801 ccgacagccg gtagtagtgg aactggttgg cgagatcggc gaggccctga cggttcgtcc
     1861 aaccgatcga tgccagctcg ccgtcggtca gcggccgggt catcagcacg tagtcgtaca
     1921 ccggcaccgt catgagccgg ttgcgcttga gcagcgacgg gaacacattg gtcgccagcg
     1981 cgacctgctt ggcccggatg ctgccgccgt cggtcaccac gtcgactccg tcactcccgg
     2041 tgtccagccg tcgcaccggg ctcgactcgt agatctcgac gcccagctcc tgcgcgacgc
     2101 gcgcgagctc gttcgcgagc cgggcgggat gcacgagcgc ggtggagcgg cggtcccagt
     2161 gggctgcgag gtaggtgggg gagttgacct cggcttgagc ggcagcggca tccattcgcc
     2221 cgtcggcatc cagccactcg atctggtgag gctcgacggc aaccgtgagg gttccggtgc
     2281 gctcgaagtc gcactgcatc gaatacctcg cgacggttgc ctcgatcgcg tcgaggttct
     2341 cgtggccgag tcggtcgagc tcgtgaagct cctcgggcca gcgacgctgg ccgttctcct
     2401 caccgtgcgt gaggctcgcc tcgacgaaac caccgttgcg accggatgcc gcccagccga
     2461 cgcgcttcgc ctcaaccacc acaacggacc ggcccgggtt ccgctccttg gcacgcacgg
     2521 cggtccacag tccggtgtat ccggcgccga cgatcacgag gtcggcggag cgggagccgg
     2581 tcagggattc gtgcggcggc ggcggtgcgt catcagtcca gaacgggcga gccaccgacg
     2641 acgcgagcga ccgcgcgatg acttcctggg ctggcagatc tcgctcgaag gctgtcgtgt
     2701 gcactgcaag gctccctatt gttgagcgtg atccgatgct atcccccgcc tcgggcagga
     2761 agtgagccga tgagtgacgc gcccctcgat ctgaccgccc tcgaggtgcc gaccgagccc
     2821 gtcgcggcat cccgcatagt cgccggctcg ccccgcgacg gcgccaccga gctgacactc
     2881 ttcgagggat gcgagatcgg ggtctgggag atgacccccg gcacggccac cgatgtcgag
     2941 gtcgacgagg t
//
LOCUS       DAOSOU010000247         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_33579_length_2950_cov_4.811744, whole genome shotgun sequence.
ACCESSION   DAOSOU010000247 DAOSOU010000000
VERSION     DAOSOU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33579_length_2950_cov_4.811744"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLW15_10845"
     CDS             <1..463
                     /locus_tag="PLW15_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOT34931.1"
                     /translation="RSLGVVRNRTPDLPVETTRVGDAGVAATIEGLTGVWRLDPEALD
                     RRFAPRTALLSPFDTLIRDRKRMADLFDFDYALEMYKPAAKRRWGYYALPILHGDRLV
                     GKVDATADPERGTLVVHAIHEDSPFTPAVGSAVRDELDALASWLGLRVAGV"
     gene            486..1427
                     /locus_tag="PLW15_10850"
     CDS             486..1427
                     /locus_tag="PLW15_10850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HOT34932.1"
                     /translation="MNESSPIEPQSVDAPLSRSAVFLTVALSNAPDAAARVRAVLGDV
                     GGLVRSVGFRDLDGLLSCIVAIGANAWDAVTGLPRPADLHPFVEVRGAKHSALATAGD
                     VFFHIRAERGDLCFELERLILAALGDAVTVVDETVGFRYFEARDLLGFVDGTENPMGS
                     ARAEAALVGDEDSEHAGGSYVVIQKYLHDLDAWNALPVEMQEQIVGRTKFDNVELDDS
                     TPRKSHKSLTTIVDDEGVEHAILRDNMPFGRPGHGEFGTYFIGYARDLWVIERMLSRM
                     FVGDPEGSYDRILDVSRAVTGVTFFAPSNDVLEGLAE"
     gene            1443..2159
                     /locus_tag="PLW15_10855"
     CDS             1443..2159
                     /locus_tag="PLW15_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT34933.1"
                     /translation="MNDLTAHDLRRLLDAAADLASVVTRARLRERAVEVLFDVMPATR
                     AGWVTMDLSTGAMSGYHLPEPIPFVVPLMPRDLAEVPLVAELLAAPVPGSLRISDTIT
                     EAEWHSRRLWAEVYRPLGGEYQIGSLLTVDEGLVETLAVFRADSDFTDRELALAEEVA
                     RQVRVTLARLRASEAREARELLTSRQRQVLAALETGATIRQAAGDLGITEKTLENHLQ
                     TIYRRLGVSSRTAALHRTFG"
     gene            2218..2865
                     /locus_tag="PLW15_10860"
     CDS             2218..2865
                     /locus_tag="PLW15_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34934.1"
                     /translation="MTLRSRVPAPSLLVTATFTLAVLILTGCSTPAPETPETPDGTAE
                     SSAPAEEDGGLSLPPQTIGSDTDCSLFSNDEIGALWGVPITDNDATQVIETGGLGGKL
                     YNCDYNETDSGLGLTVDLEYREYDSVDGAIQYMTDIRNGSSFEGTIYYELDEVPGLGD
                     EAFFSIDPDLSGAEAAVLLLYTRTDNLMLLLSATNLEGVKPEYRDLLVETYKLKF"
BASE COUNT          460 a         1065 c          951 g          474 t
ORIGIN      
        1 gcgctcactc ggcgtcgtgc gcaaccgcac gcccgacctg cccgtggaga cgacgcgcgt
       61 gggcgacgct ggggtcgcgg ccacgatcga ggggctgacc ggcgtgtggc ggctcgaccc
      121 cgaggccctc gaccggcgct tcgcgccccg aaccgcgctg ctctccccct tcgacaccct
      181 gatccgtgat cgcaagcgca tggccgacct gttcgacttc gactacgcgc tcgagatgta
      241 caagcccgcc gcgaagcgcc gctggggcta ctacgcgctg ccgatcctgc acggtgaccg
      301 tctggtgggc aaggtggatg ccaccgccga ccccgagcgc ggcaccctcg tcgtgcacgc
      361 catccacgag gactcgccct tcacgccggc agtgggatcg gccgtgcgcg acgagctcga
      421 cgctctcgcc tcgtggctgg ggctgcgcgt cgcgggagtc tgattcgcgc tgctaccgtg
      481 gcgtggtgaa cgagtccagc ccgatcgaac cacagagtgt cgatgcgccc ctgtcccggt
      541 ccgccgtgtt cctcacagta gctctcagca acgccccgga tgccgcagca cgagttcgcg
      601 ccgtgctcgg cgacgtcggc gggctcgtgc gctcggtcgg gttccgcgac ctcgacggcc
      661 tgctctcctg catcgtggcg atcggggcca acgcctggga tgccgtcacc ggcctccccc
      721 ggcccgccga ccttcatccg ttcgttgagg tgcgcggggc caagcacagc gcgctcgcga
      781 ccgcgggcga cgtgttcttc cacatccgcg ccgagcgcgg cgacctctgc ttcgaactgg
      841 agcggctcat cctcgccgca ctcggcgacg cggtcaccgt cgtcgacgag actgtcggct
      901 tccgctactt cgaggcccgc gacctgctcg ggttcgtcga cggcaccgag aacccgatgg
      961 gctcggcccg cgccgaggcg gcgctggtcg gcgacgaaga ctccgagcac gccggcggca
     1021 gttacgtcgt catccagaag tacctgcacg acctggacgc ctggaacgcc ctgcccgtcg
     1081 aaatgcagga gcagatcgtc gggcgcacca agttcgacaa cgtcgagctc gacgactcga
     1141 ctccccgcaa gtcgcacaag tcgctcacca cgatcgtcga cgacgagggc gtcgagcacg
     1201 ccatcctgcg cgacaacatg cccttcggcc gccccggtca cggcgagttc ggaacctact
     1261 tcatcggcta cgcccgtgac ctgtgggtga tcgaacggat gctcagccgc atgttcgtgg
     1321 gcgaccccga gggctcgtac gaccgcatcc tcgacgtctc ccgggcggtg accggggtca
     1381 cgttcttcgc cccgtcgaac gacgtgctgg aaggtctcgc cgagtgagcg cccgatagcc
     1441 ccgtgaacga cctgactgcc cacgacctcc gacggttgct ggatgccgcg gccgacctcg
     1501 cctcggtggt cacccgtgca cggctgcgcg agcgagccgt ggaggtgctg ttcgacgtga
     1561 tgcccgcgac gcgcgccggc tgggtgacga tggacctgtc gaccggcgcg atgagcggct
     1621 atcacctgcc ggaaccgatc ccgttcgtcg tgccgctcat gccgcgcgat ctggcggagg
     1681 tgcccttggt ggccgaactg ctagccgcgc ccgtgcctgg ctcgctgcgc atcagcgaca
     1741 cgatcaccga ggcggagtgg cattcccggc ggctctgggc cgaggtgtac cggccactcg
     1801 ggggcgagta tcagatcggc tcgctgctca ccgttgatga ggggctggtc gagaccctcg
     1861 ccgtgttccg tgccgacagc gacttcaccg atcgcgagct ggcgctggct gaggaggtcg
     1921 cccggcaggt gcgggtcacg ctcgcacggc tccgcgcgag cgaggcccgc gaggcccgcg
     1981 aactgctgac atcccgtcaa cggcaggtgc tcgccgcact ggagacgggc gcgacgatcc
     2041 ggcaggctgc gggcgatctc ggcatcaccg agaagaccct cgagaatcac ctccagacga
     2101 tctaccgacg gctgggtgtg tcgagtcgca cggccgcgct ccaccgcacg ttcggctgac
     2161 gggtttgggg gttttcccct atcgaagcgg acgatgccgc ttccatacac tgcgatcatg
     2221 accctgcgct cgcgtgttcc cgcccccagc ctcctcgtta cagccacctt cacactggcg
     2281 gtcctgatac tcaccggctg cagcaccccg gcacccgaga caccggagac gcccgacggc
     2341 actgccgagt cgagcgcgcc ggccgaggag gacggcggcc tcagcctccc gccgcagacc
     2401 atcggaagcg acaccgactg ctcactcttc agcaacgacg agatcggcgc gctgtggggc
     2461 gtgccgatca ccgacaacga tgcgacccag gtcatcgaga cgggcggcct cggcggcaag
     2521 ctgtacaact gcgactacaa cgagaccgat tccggcctcg gcctgacggt ggacctcgag
     2581 tatcgcgaat acgactcggt cgacggcgcg atccagtaca tgaccgacat ccgcaacggc
     2641 tcgtcgttcg aggggacgat ctactacgag ctcgatgagg ttccagggct aggtgacgag
     2701 gccttcttca gcatcgatcc cgacctctcc ggagcggagg ccgccgtcct gctcctctac
     2761 acccgcaccg acaacctgat gctcctcctc agcgccacca atctcgaggg cgtgaagccg
     2821 gagtatcgtg acctactcgt cgagacctac aaactgaagt tctagatgtc gcccgcaccc
     2881 atcatgatgg gttagtgacg cgacgcggcc ttctgctctt cagcgccctc gggctgatct
     2941 ggggcatccc
//
LOCUS       DAOSOU010000248         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_33867_length_2929_cov_5.325678, whole genome shotgun sequence.
ACCESSION   DAOSOU010000248 DAOSOU010000000
VERSION     DAOSOU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33867_length_2929_cov_5.325678"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLW15_10865"
     CDS             complement(<1..873)
                     /locus_tag="PLW15_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT34935.1"
                     /translation="MSLQIECTDLVKIYTAEGVEVQALQGLTLRVERGELTAIVGASG
                     SGKSTLLSILTGLEIPTAGRAMVAGHDLLAMTSAQRTRYRRETVGFVWQQTSRNLLPY
                     YSAAQNIAMPLAIAGVPRAQRSARVDELIELLGMGDFARRHPAQLSGGQQQRVAIAVA
                     LANEPEVLFADEPTGELDEVNSQVVFDALGSVNRELGVTVLIVTHDEEVSTKVRRTVQ
                     IRDGRLSTEVHRSTETDEFGEEQHRSQEFVVLDRVGRLQLPQEYVRTLGLQGLVRLEL
                     ADDHAQVHPTETGKA"
     gene            complement(870..>2929)
                     /locus_tag="PLW15_10870"
     CDS             complement(870..>2929)
                     /locus_tag="PLW15_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT34936.1"
                     /translation="PDDSYWRHAFDLGSPIEIRQSGQRPTIQASAFVDPETLVDLQEP
                     FASGTIAAWTPIDPQAYSYADLDELSTQAGNLVVTPRSLPEFGQLSLSTALPELLERT
                     RQAVSATSALITLAASGLLGVLIATYALSLRALVRRRHSALALLSARGAASGQIRGLV
                     VLEAAIVALPGSAVAIGAAALLAPERIGLDGWLAPVALAITPVILAAVLVAPSNLRET
                     RQDVAVRSTWPLRWVLEVAVAGAAVIALILLQRRGLVASSDIAAVDPLLSATPLLVAA
                     TAGLVALRLFPLPVRAVREMARTRTAPVWEVGSARAAREPAIGAIATLALVIGVTIVV
                     FSTVMMSTVAATMAGAVRERLGADMEIVAHDLPAPVVDQIERLPGVTGVVTLTSRSGL
                     TVMDDAGPITVTVVLADPAALHRVRPDLPQLTGASAGTAPVILSAGLAERVQGTDIAI
                     EENPVSVVGVVSDTALPGPTGLWVVADQATAGDLGLGDLTPTRMLIALDDEQDAATVD
                     EITGIVLDAQSGSFAESARVSDIQSELDQSQADPITFALQAALLIVAAASLLLTVLVV
                     TLASAVSATSRNRVLGVLRILGMTPRQVRGLVAWEFGPVAVASIVVGTLVGIGLPYLV
                     TSVLDLRAFFGGTVLPQPSLEPVWIAAAVGGYALAIVVAVLVASALGRRFAPASTLKM
                     GEP"
BASE COUNT          484 a         1025 c         1023 g          397 t
ORIGIN      
        1 cgccttcccg gtctcggtcg gatgcacctg cgcgtgatca tcggccaact cgagccgcac
       61 cagcccctgg agaccgaggg tgcgcacgta ctcctgcggc agctggagtc gaccgacgcg
      121 gtcgagcacc acgaactcct gggagcgatg ctgctcctcg ccgaattcgt cggtctcggt
      181 gctgcgatgc acttcggtcg acagccgccc gtcgcgaatc tgcacggtgc gcctcacctt
      241 ggtcgacacc tcctcgtcgt gagtgacgat gagcaccgtg acccccagtt cgcgattgac
      301 cgaaccgagc gcgtcgaaca ccacctgcga gttgacctcg tcgagttcgc cggtcggctc
      361 gtcggcgaac agcacttcgg gctcgttggc gagcgcgaca gcgatggcga cgcgctgctg
      421 ctgcccgccg gagagctgcg ccggatgccg ccgagcgaag tcgcccatgc ccagaagctc
      481 gatgagctca tccacccgcg ccgacctctg tgctcggggc acgccggcga tcgccagcgg
      541 catggcgatg ttctgggcgg ccgagtagta gggcagcagg ttgcgggagg tctgctgcca
      601 cacgaacccg accgtctcgc gccggtagcg cgtgcgctgc gcagaggtca tcgcgagcag
      661 atcgtggccc gcgaccatcg cccggcccgc cgtggggatc tcgagtccgg tgaggatcga
      721 cagcagcgtc gacttgccgg agccggaggc gccgacgatc gccgtgagct ccccgcgctc
      781 cactcgcaga gtgaggccct gcagggcctg cacctcgacg ccctcggcgg tgtagatctt
      841 caccaggtcg gtgcactcga tctgaagact catggttctc ccatcttgag ggtggatgcc
      901 ggcgcgaacc gacgaccgag ggcgctcgcc accagcaccg cgaccacgat cgccagggcg
      961 tagccgccca ccgccgccgc aatccacacc ggttccagcg acggctgggg cagcacggtg
     1021 cccccgaaga acgcgcgcag gtcgagcacc gaggtgacga gatagggcag gccgattccc
     1081 accagggttc ccacgacgat cgacgcgacc gcgaccgggc cgaactccca ggcgacgagc
     1141 ccgcgcacct ggcggggcgt catcccgagg atgcgcagca cgccgagcac acggttgcgc
     1201 gaggtcgccg acaccgccga cgccagggtc accacgagca ccgtgagcag gagcgaggcc
     1261 gcggccacga tgagcagggc ggcctgaagg gcgaaggtga tcgggtcagc ctggctctgg
     1321 tccagctcgg actggatgtc agaaactcgc gcagactcgg cgaagcttcc cgattgcgca
     1381 tcgagcacga tcccggtgat ctcatcgacc gtcgcggcat cctgttcgtc gtcgagcgcg
     1441 atgagcatgc gggtcggtgt caggtcgccg agcccgaggt ccccggcggt cgcctggtcg
     1501 gcgacgaccc acagcccggt cggtccggga agggcggtgt cggagaccac tccgaccaca
     1561 ctcacggggt tctcctcgat cgcgatgtcc gttccctgga ctcgctcggc gagacctgcc
     1621 gacaggatca ccggcgctgt gccggcggac gcgccggtga gctgcggcag gtcgggtcgc
     1681 acccggtgaa gggcagccgg gtcggccagc accaccgtca cggtgatcgg ccccgcgtca
     1741 tccatcacgg tcagcccgct gcgcgacgtg agggtgacga ccccggtgac cccgggcagc
     1801 ctctcgatct gatcgacgac gggagccggc aggtcatggg cgacgatctc catgtcggcg
     1861 ccgagcctct ccctgacggc gcccgccatc gtcgcagcga ccgtgctcat catcaccgtg
     1921 gagaagacga cgatggtgac gccgatcacc agtgcgaggg tggcgatggc tccgattgcg
     1981 ggttctcgtg cggcgcgcgc agaacccacc tcccacaccg gggcggttcg ggtgcgggcc
     2041 atctcccgca ccgcacgcac gggcagtggg aacagccgca gggcgaccag gccggccgtc
     2101 gccgccacca gcaacggtgt agccgagagc agcggatcga ccgcagcgat gtcgctcgac
     2161 gcgacgagcc cccgccgctg cagaagaatg agcgcgatca ccgcggcgcc cgcgaccgcc
     2221 acctcgagaa cccagcgaag cggccacgtc gaccgcaccg cgacatcctg tcgggtctcg
     2281 cggaggttgc tcggcgccac gagcaccgcg gcgaggatca ctggtgtgat cgcgagagcg
     2341 accggggcga gccagccatc gaggccgatg cgttcgggag ccagcaacgc cgccgccccg
     2401 atcgccacgg ccgatccggg gagagcaacg atcgcggcct cgagcaccac gaggccccgg
     2461 atctgaccgg aagcggctcc ccgcgccgac agcagggcga gcgccgagtg acgtcggcgc
     2521 acgagcgccc gcaggctcag agcgtacgtt gcgatcaaga ccccgaggag cccggatgcg
     2581 gcgagcgtga tcagggcaga cgtcgccgag accgcctgtc gcgtgcgctc cagcaattcg
     2641 ggcagcgcgg tgctgagact gagctgaccg aactcgggca ggctgcgcgg ggtgacgacc
     2701 aggttgcccg cctgagtcga cagctcgtcc aggtcggcat acgagtaggc ctggggatcg
     2761 atcggcgtcc acgccgcgat ggtgccgctc gcgaagggtt cctgcaggtc gaccagagtc
     2821 tcggggtcga cgaaggccga cgcctgaatc gtaggccgtt gcccggactg gcgtatctcg
     2881 atcggcgagc cgaggtcgaa ggcgtggcgc cagtagctgt cgtcgggct
//
LOCUS       DAOSOU010000249         2868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_34839_length_2868_cov_6.116246, whole genome shotgun sequence.
ACCESSION   DAOSOU010000249 DAOSOU010000000
VERSION     DAOSOU010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34839_length_2868_cov_6.116246"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLW15_10875"
     CDS             <1..983
                     /locus_tag="PLW15_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOT34937.1"
                     /translation="DDRFNTTQAMREAVFFDGPEANRRYSRFWLLLILAAVIASAGVV
                     ADSTATVIGAMIVAPLMTPILGTMLATVLGDHTNLVRSLLLVLAGAATAIAIGYLVGS
                     LVAVDVLAETNPQVAGRVTPGLIDLLAALGTGVVGSVALVRRDISDTLPGVAIAISLV
                     PPLAVVGLALESGSVIQALGALLLFLTNFVAIIGTGIVVMSIYGVNRLARVDSAGKPR
                     RLARPIALLAVMLVIIGAPLAYTSLTVAARSIVEKGVFDTSERWAQSVGWEVQDVTTQ
                     GEKVHVRLEGPLPLPQTSELKSALDAAGVDTSLVIVNLVPIYTVDLDAIE"
     gene            970..2313
                     /locus_tag="PLW15_10880"
     CDS             970..2313
                     /locus_tag="PLW15_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HOT34938.1"
                     /translation="MRLNERLSSRTPQALLPLVQQPAVDSFLDWMFFIVGGASAVWLA
                     ALLFGESFTLGWGQIWFFLVFWVLLAYLVLPRLHRILTRIYVPEYFIGRARTSDGLLG
                     DPINLALLGSEPQVHDAMTAAGWTRADDLGLATGWRIVASTLMRRSYLEAPVSPLLLF
                     GRKQDFAYQQEVNGTPGKRHHVRFWRCPPDWLLPGGIRVDWLAAGTYDRAVGFSLFTL
                     QITHKIDARTDVERDHIVDTVTAASRRVRVNVIRDFSTGYHARNGGGDAIVTDGDLPI
                     IDISRVRTVGTEMVPTDSRDKRPSPTIVGALLVFSRALVTLVAAAAVFVDRNALDIAP
                     GSDPQAVEVVRWVIVGALLLFAAADILLAWFVFLGRNWARVLVMTLSTLAIVVQAVGW
                     ATGTTTLTLATNLPGLSVDILLILALSSERALTYARRTRKVPKRISGRPGDSAAI"
     gene            complement(2310..>2868)
                     /gene="pdxH"
                     /locus_tag="PLW15_10885"
     CDS             complement(2310..>2868)
                     /gene="pdxH"
                     /locus_tag="PLW15_10885"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOT34939.1"
                     /translation="QARLWLDQAAESNVYEPNAMVLSTIDPDGLPSSRTVLLRALDER
                     GLEFFTSYTSAKGRALLAHPEVSIVFPWYLIHRQIIVRGVAAPVDPAVSEAYFASRPR
                     GSQIAATASDQSRPIASRDELERRVEELEVKYDGRDVPRPDTWGGFRVVPESIEFWQG
                     RSSRLHDRLKFTRSGSGWTMERLQP"
BASE COUNT          410 a          993 c          966 g          499 t
ORIGIN      
        1 tggacgatcg gttcaacacc acgcaggcca tgcgcgaggc cgtgttcttc gacggccccg
       61 aggccaaccg ccgctacagc aggttctggc tgctgctcat cctcgcggcg gtcatcgcct
      121 ccgcgggtgt cgtcgccgac tccactgcga cggtcatcgg ggccatgatc gtggcaccgc
      181 tcatgacacc gatcctcgga accatgctcg ccacggtgct cggcgaccac actaacctcg
      241 tgcgatcgtt gctgctcgtg ctcgcggggg ctgcgacggc catcgccatc ggatacctcg
      301 tgggcagtct ggtcgccgtc gacgtgctcg ccgagaccaa cccgcaggtc gcgggacggg
      361 tcacgcccgg gctcatcgac ctgctcgccg ccctcggcac gggcgtcgtg ggatcagtag
      421 cgctcgtgcg ccgcgacatc tccgacacgc tgccgggtgt ggccatcgcg atctcgctcg
      481 tgccgccgct cgccgtcgtc ggtctcgcgc ttgagtcggg ttcggtcatc caggctctcg
      541 gcgccctgct gctcttcctc accaacttcg tggcgatcat cggaaccggc atcgtggtca
      601 tgtcgatcta cggcgtgaac cgcctcgccc gcgtcgactc ggcgggcaag ccccggcggc
      661 tcgcccgccc gatcgctctg ctcgccgtca tgctcgtcat catcggtgcc ccgctcgcct
      721 acacgagcct caccgtcgcc gcccgctcca tcgtggagaa gggcgtgttc gacaccagcg
      781 agaggtgggc ccagtcggtg ggctgggagg ttcaggacgt cacgacccag ggcgagaagg
      841 tgcacgtgcg cctcgagggt ccgttgccgc tgccgcagac atccgagctg aagagtgccc
      901 tggatgccgc gggggtagac acctcgctgg tgatcgtgaa cctcgtaccc atctacaccg
      961 tggacctcga tgcgattgaa tgagcggctg agctcgcgca ccccgcaggc cctgctgccg
     1021 ctggtgcagc agccggcggt cgactccttt ctcgactgga tgttcttcat cgtcggcgga
     1081 gcgtcagccg tctggctcgc cgcgctgctg ttcggggaga gcttcacgct cgggtggggt
     1141 cagatctggt tcttcctggt gttctgggtg ctgctggcct acctcgtgct cccgcggctg
     1201 caccgcattc tcacccgcat ctacgtgccc gagtacttca tcgggcgggc ccgcaccagc
     1261 gacggtctgc tgggagaccc catcaacctc gcgctgctgg gcagcgagcc gcaggtgcac
     1321 gacgcgatga cggcggcggg gtggacgcga gccgacgacc tggggctcgc gacggggtgg
     1381 cggatcgtgg catccacgct gatgcggcgc agctatctgg aggccccggt cagcccgctg
     1441 cttctgttcg ggcgcaaaca ggacttcgcg taccagcagg aggtcaacgg cacccccggc
     1501 aagcgccacc acgtgcggtt ctggcggtgc ccgccggatt ggttgctgcc cgggggtatt
     1561 cgggtcgact ggctcgcggc cggtacctac gaccgggcgg tcgggttctc gctgttcacg
     1621 ctgcagatca cccacaagat cgacgcgcgc accgatgtcg agcgcgacca catcgtcgac
     1681 acggtgactg cggctagtcg acgggtgcgg gtcaacgtca tccgggactt ctcgaccgga
     1741 taccacgctc gcaacggcgg gggagacgca atcgtcaccg acggcgacct gcccatcatc
     1801 gacatcagcc gggtgcgcac ggtcggaacc gagatggtgc ccaccgacag tcgagacaag
     1861 aggcccagtc cgacgatcgt cggggcgctg ctggtcttct cccgagccct ggtcacgctg
     1921 gtcgcggccg cggcggtctt tgtcgatcga aatgctctgg acatcgcccc gggcagcgac
     1981 ccgcaagccg tcgaggtcgt gcgctgggtg atcgtgggcg ctctgctgct gttcgccgcg
     2041 gccgacatcc tgttggcctg gttcgtgttc ctcgggcgca actgggctcg tgttttggtg
     2101 atgaccctca gcacactggc gatagtcgtg caggcggtgg gctgggcgac cggcaccacg
     2161 accctcaccc tggcgacgaa cctgcccggg ctgtcggtcg acatcctgct gatcctggcg
     2221 ctgtcgagcg aacgtgcgct gacttacgcc cgccgtacgc gcaaggtgcc caagcgcatc
     2281 tccggcaggc cgggcgactc cgccgccatc tagggctgga gacgttccat cgtccagccc
     2341 gatccggagc gggtgaactt caggcggtcg tgaaggcgcg acgatcgacc ctgccagaac
     2401 tcgatcgact cgggcacgac tcggaagcca ccccaggtgt cgggtcgggg cacgtcgcgc
     2461 ccgtcatact tcacctcgag ctcttcgacc ctgcgctcga gctcgtcgcg ggaggcgatg
     2521 ggccgggact ggtcgctcgc cgtggccgcg atctgcgatc ctcgcggacg gctggcgaag
     2581 tacgcctccg agacggccgg gtcgacgggc gccgcaaccc cgcgcacgat gatctggcgg
     2641 tggatgaggt accaggggaa cacgatcgag acctcgggat gcgccagcag cgccctgccc
     2701 ttggccgacg tgtagctcgt gaagaactcc agcccgcgct cgtcgagggc ccgcagcagc
     2761 accgtacggc tggacggcag tccgtcggga tcgatcgtcg agagcaccat cgcgttcggc
     2821 tcgtagacgt tcgactcggc ggcctggtcg agccatagcc gggcctgc
//
LOCUS       DAOSOU010000250         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_35062_length_2855_cov_5.985714, whole genome shotgun sequence.
ACCESSION   DAOSOU010000250 DAOSOU010000000
VERSION     DAOSOU010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35062_length_2855_cov_5.985714"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLW15_10890"
     CDS             <1..1164
                     /locus_tag="PLW15_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT34940.1"
                     /translation="EPIVELNRNLGAMPLVGGNNAELLPDYEKSLQAMTDAVGEATKS
                     VHVEFYILSLDHTTAPFFDALEAAVKRGVDVRVLLDHIASIRSVGHRKTARRLTKMGA
                     QWHYMLPVRPLRGQWQRPDLRNHRKLLIIDGKVGFTGSQNFIDSSYNKKGNIRRGLHW
                     KDLMVRFEGPIVAGIDALFATDWYCETDELILREHDVPRPVKESPIDAQVVPSGPGFE
                     GENNLRLFNALVYAATKQVIITSPYFVPDGSMLYAITTAAQSGLDVQLFASEIGDQAV
                     VYHAQRSYYEELLRAGVRIWLYKAPTVLHAKFFTIDDEVAVVGSSNMDMRSFALDLEI
                     SVMVRGRTIMDDLHELQEQYRENSRELTLDEWMTRSRVAATFDNVARLAATLQ"
     gene            complement(1177..1905)
                     /locus_tag="PLW15_10895"
     CDS             complement(1177..1905)
                     /locus_tag="PLW15_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT34941.1"
                     /translation="MTDRLDPARLDPARLDELWDFADAAASADRFRTEIASAPPVAAD
                     ELRTQLARALGLAGRGDDADALLDSIASDDLVVRARVALERGRRRNSSGHPDEAAPHF
                     EQALELAQSAHEDFLAVDALHMLAIADQAGSAEWAVQGVALAEASADPRTHRWLIALH
                     NNLGWLHHDAGRYPEALAEFEAADAAARAHGSEDQQQIAQWAIARALRSLGRRDEARA
                     IQERLAVLRPDDEYVTEELEALKE"
     gene            complement(1937..2431)
                     /locus_tag="PLW15_10900"
     CDS             complement(1937..2431)
                     /locus_tag="PLW15_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT34942.1"
                     /translation="MTFTAQSIEPESIDLATLATLAGDLSSEYLLQRIRERGHPFVRT
                     AHGYLIQHLVAGSPTVTELASRMGVTQQAASKSVVELETLGLVQREGEAGDQRVRRVT
                     LTDAGREVVESGRAARAHLEAKLVEALGDDVIATAKGALAALIDAVGGIDDLRGRRVR
                     ARTV"
     gene            2483..>2855
                     /locus_tag="PLW15_10905"
     CDS             2483..>2855
                     /locus_tag="PLW15_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT34943.1"
                     /translation="MTDTTTALTALHDALTAGRHGEDLRELFTPDATTTEHPNALNPR
                     GRTSTLAEMLAASEAGAGLLAWQRYDAHTLAEHGTTVVARLTWTGEVARDAGPFREGQ
                     RLTAHIAQFAEVEGGRIRSIET"
BASE COUNT          461 a         1006 c          962 g          426 t
ORIGIN      
        1 gagccgatcg tcgaactcaa ccgcaacctc ggcgcgatgc cgctggtcgg cggcaacaac
       61 gccgagctgc tgcccgacta cgagaagtcg ctccaagcga tgacggatgc cgtcggcgaa
      121 gccacgaagt cggtgcacgt cgagttctac atcctgtcgc tcgaccacac gaccgccccc
      181 ttcttcgatg cgctcgaggc ggccgtgaag cgcggcgtcg acgtgcgcgt gctgctcgac
      241 cacatcgcat ccattcgctc ggtcggccac cgcaagaccg ccaggcggct cacgaagatg
      301 ggcgcgcagt ggcactacat gctgcccgtg cgaccgctgc gcggccagtg gcagcgcccc
      361 gacctgcgca accaccgcaa gctgctcatc atcgacggaa aggtcggctt cacgggatcg
      421 cagaacttca tcgactcgag ctacaacaag aagggcaaca tccgtcgagg tctgcactgg
      481 aaggacctca tggtgcgctt cgagggaccg atcgtcgccg gtatcgacgc gctgttcgcg
      541 accgactggt actgcgagac cgacgagctg atcctgcgcg agcacgatgt tccccgcccg
      601 gtgaaggaga gcccgatcga cgcgcaggtc gtgccgagcg gcccgggctt cgagggcgag
      661 aacaacctca ggctgttcaa cgccctggtg tacgccgcca ccaagcaggt catcatcacg
      721 agcccctact tcgtgcccga cgggtcgatg ctctacgcga tcaccacggc ggcgcagagt
      781 ggcctcgacg tgcagctgtt cgcctcggag atcggcgacc aggccgtcgt ctaccacgcc
      841 cagcgaagct actacgagga gctgctgcgc gccggcgtgc gcatctggct gtacaaggcg
      901 cccaccgtgc tgcacgccaa gttcttcacc atcgacgacg aggtcgcggt ggtcgggtcg
      961 agcaacatgg acatgcgctc gttcgcactc gacctcgaga tctcggtgat ggtgcgcggc
     1021 cgcacgatca tggacgacct gcatgagctt caggagcagt accgcgagaa cagtcgcgag
     1081 ctgaccctcg acgagtggat gacgcgctcg cgcgtcgcgg cgacgttcga caacgtggcg
     1141 cgcctcgccg ccacgctgca gtaacgcggc cgcgcgctac tccttcagcg cctcgagctc
     1201 ctcggtcacg tactcgtcgt cggggcgcag caccgcgagc cgctcctgga tggcgcgggc
     1261 ctcgtcccgt cgccccagcg atcgcagcgc gcgggcgatg gcccactggg cgatctgctg
     1321 ctggtcctcg ctgccgtggg cgcgcgccgc ggcatccgcc gcctcgaact cggcgagagc
     1381 ctcggggtag cgaccggcgt cgtggtgaag ccagccgagg ttgttgtgca gtgcgatgag
     1441 ccagcggtgg gtgcgggggt cagcggatgc ctcggcgagc gccacgccct gaacagccca
     1501 ctcggccgat cctgcctggt cggcgatcgc gagcatgtgc agggcatcga cggcgagaaa
     1561 gtcttcgtgc gcggactgcg cgagttcgag cgcctgctcg aagtgcggcg cagcctcgtc
     1621 gggatgcccc gacgagttgc gccgacgccc ccgctcgagt gcgacgcggg cccgcaccac
     1681 gaggtcgtcg gatgcgatcg agtcgagcag cgcgtctgcg tcgtcgccgc ggcctgcgag
     1741 gcccagcgcg cgggccagct gcgtgcgcag ttcgtcggcc gcgacgggtg gagcggacgc
     1801 gatctcggtg cggaaacggt cggcgctggc cgcggcatcc gcgaagtccc agagctcgtc
     1861 gaggcgtgcg gggtcgagac gtgcagggtc gagtcgatcg gtcacccgac cagcgtagcc
     1921 acggtgccgc gcgcggtcac accgttcggg cgcgcacccg tcgtccgcgc aggtcgtcga
     1981 tgccgccgac cgcgtcgatg agcgcggcga gcgcaccctt ggcggtggca atgacatcgt
     2041 cgccgagcgc ctcgacgagc ttcgcctcga ggtgggctcg cgctgcgcgc ccggactcga
     2101 caacctcacg gccggcgtcg gtgagcgtca cccgacgcac ccgctgatcg cccgcctcac
     2161 cctcgcgctg caccaacccg agggtctcga gctcgaccac cgacttcgac gcggcctgct
     2221 gggtgactcc catgcgcgag gcgagctcgg tgacggtcgg tgagccggcg accagatgct
     2281 gaatgaggta accgtgggcg gtgcgaacga acggatgccc gcgctcccgt atgcgctgca
     2341 gcaggtactc cgaactcagg tcgccggcga gcgtggcgag ggtcgccagg tcgatggact
     2401 ccggttcgat gctctgcgct gtgaacgtca tggttccact ctagcttgac aaccttggtt
     2461 gtacaactta tgttgtgttt ccatgacgga caccactacc gcgctcaccg cgctgcacga
     2521 cgccctgacc gccggacgac acggcgaaga cctgcgtgaa ctcttcaccc cggatgccac
     2581 caccacagag caccccaacg ccctcaaccc gcgcggccgc accagcacgc tcgccgagat
     2641 gctcgcggct tcagaggccg gcgccgggct gctcgcctgg cagcgctacg acgcgcacac
     2701 cctcgccgaa cacggcacga ccgtcgtcgc gcgcctgacc tggaccggcg aggtggcccg
     2761 cgacgcgggt ccgttccgcg aagggcaacg gctcaccgcg cacatcgccc agttcgccga
     2821 ggtcgagggt gggcgcatcc ggtcgatcga gacgt
//
LOCUS       DAOSOU010000251         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_35775_length_2810_cov_5.132486, whole genome shotgun sequence.
ACCESSION   DAOSOU010000251 DAOSOU010000000
VERSION     DAOSOU010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35775_length_2810_cov_5.132486"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..819
                     /locus_tag="PLW15_10910"
     CDS             19..819
                     /locus_tag="PLW15_10910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT34944.1"
                     /translation="MPHESLLTTARTIALRAGELARLRRSEGVAVAASKSSPVDIVTE
                     ADRETEALIRGLLADERPGDGFFGEESGAERGTTGLTWIVDPIDGTVNFLYGIPHYAV
                     SIAVVEGEPDPQTWTALAGCVVNPASGEVYTATAGGGARLGETPIRVADAVPLSQALV
                     ATGFAYDATTRGEQGRLVADLLPLVRDIRRLGTASLDLCFVASGRTNAYFERTLSPWD
                     HAAGALIAREAGATVKGLGDAQPSRDLILAGHPDVAAALEGALAQLAR"
     gene            951..1892
                     /locus_tag="PLW15_10915"
     CDS             951..1892
                     /locus_tag="PLW15_10915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT34945.1"
                     /translation="MSLRELDGPATTSPAPLTRREAREREEAARSAQAKAIYSSAAPA
                     RQEPVQPRVQAPELHSARRQNSAPPPPKAPGAPRKATPAKRHTGSRLLSLGALLFAGT
                     LLVAVSVPANAFMSDASVLAPSVATTALKGQSLVVAEDAAAPDLTRDGYSVTSYAQLL
                     RLKYGNAGTFTATTGAIRWPFPYSVPTTDGFGPRDISISGRSFHNGVDFTPGAGTPIY
                     AIADGVVTLHANDYGGLGNNVMISHVINGQNIDSVYAHMQEDSSPLVVGETIKVGDFV
                     GLVGDTGTSYGAHLHFELHVDKVPVDPFAWLTANAVN"
     gene            complement(1911..>2810)
                     /locus_tag="PLW15_10920"
     CDS             complement(1911..>2810)
                     /locus_tag="PLW15_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT34946.1"
                     /translation="VHDIWRGWHKVLAEYEGDRVLCAEAWVDPLEKVALWVRPDEMSQ
                     AFNFGYMFTDWKAPALKRIITESLKAFGEVGAPSTWVLSNHDVVRHASRLALTADSPQ
                     GAGIGPKSPGKPNEAIGLRRARAATSLMLALPGSSCIYQGEELGLPEDMELPDAARQD
                     PTWFRTHGEKYGRDGCRVPIPWEADRPAFGFNHTGDSWLPQPTDWGRFARDAQVGVPG
                     STLELYTLALRLRREHLLASGALQWLKGYGRSVVAFRNGDITVIANLGKVAVELPVGE
                     VLLASETITGGALPTDTTVWIRS"
BASE COUNT          425 a         1021 c          937 g          427 t
ORIGIN      
        1 actgactacg ctggagccgt gccccacgag tcgctgctca ccaccgcccg aacgatcgcg
       61 ctgagggccg gtgagctggc gcggctgcgg cgcagcgaag gggttgcggt tgcggcatcc
      121 aagtcgtcgc ccgtcgacat cgtcaccgag gccgaccggg agaccgaggc gctgatccgc
      181 ggactgctcg ccgacgagcg gccaggcgac ggcttcttcg gcgaggagag cggggccgag
      241 cgcggcacca cgggcctcac ctggatcgtc gacccgatcg acggcacggt caactttctc
      301 tacgggatac cgcactacgc cgtctcgatc gccgtggttg agggcgagcc cgacccgcag
      361 acctggacgg cgctcgccgg atgcgtcgtc aacccggcgt cgggcgaggt ctacacggcg
      421 accgcgggtg gcggggcccg gctcggcgag acgcccatcc gggtcgcgga tgccgtgccc
      481 ctctcccaag cgctcgtcgc caccggcttc gcgtacgacg ccaccacgcg gggggagcag
      541 gggcgactcg tggccgacct gctgccgctg gtgcgtgaca tccgccgcct cggtaccgcc
      601 tcgctcgacc tgtgcttcgt cgcgtcgggg cgcaccaacg cctacttcga gcgaaccctc
      661 agcccgtggg atcatgcggc gggcgcgctg atcgctcgcg aggcgggagc caccgtcaag
      721 ggtctcggtg acgcccagcc gagtcgcgac ctgatcctgg ccggccaccc ggatgtcgcc
      781 gccgccctcg agggagccct cgcgcaactc gcccgctgac ccgctcggct ggcgtctcac
      841 agggagagcg ggtacccttt acctatccgt tataagcgcg cgcatcgagc gcgctgaaac
      901 aagaatcccg tcgacagtcc cgacccacga ctgttgtgta ggagtccgtg atgtccctgc
      961 gtgagctaga cggcccggcg accacgtcgc cagcgccgct gactcggcgc gaggcgcgcg
     1021 agcgcgagga ggctgcgcga tccgcgcagg ccaaggcgat ctacagttcg gccgcgcccg
     1081 cccggcagga gccggtccag ccgcgagttc aagctcctga gcttcacagc gcgcggcgcc
     1141 agaactctgc tccgcccccg ccgaaggctc ctggggcgcc tcgcaaggcg actcccgcga
     1201 aacgccacac cggttcccgg ctgctgtcgc tcggggcact cctgttcgct ggcaccctcc
     1261 tcgtcgcggt gtcggtgccc gcgaacgcat tcatgagcga tgcctcggtg ctcgcgccat
     1321 cggtcgcgac cacggcgctg aaaggtcaga gcctcgtcgt cgccgaagac gcggcagcgc
     1381 cagatctcac gcgcgacggg tactcggtca cctcctacgc ccaacttctt cgtctcaagt
     1441 acggcaacgc tggcaccttc actgctacca cgggtgcgat tcggtggccc ttcccctact
     1501 cggtgccgac taccgacggc ttcggtcccc gtgacatcag catctccggc aggtcgttcc
     1561 acaacggcgt cgacttcacc cccggtgccg gtacacccat ctacgcgata gccgacggtg
     1621 tggtgaccct gcatgccaac gactacggcg gcctcggcaa taacgtgatg atcagccacg
     1681 tgatcaacgg gcagaacatc gacagcgtct acgcgcacat gcaggaggac tcgagcccgc
     1741 tcgtcgtcgg ggagaccatc aaggtcggcg acttcgtcgg gctggtcggc gacacgggca
     1801 cctcctacgg tgcgcacctg cacttcgagc tccacgtcga caaggtgccc gtcgacccct
     1861 tcgcgtggct caccgccaac gcggtcaact gacacgcggc gcgatctacc tcagctgcga
     1921 atccacaccg tcgtgtcggt gggcagcgcc ccgccggtga tcgtctcgct ggcgagcagc
     1981 acctcgccga ccggcagctc gacggccacc ttgccgaggt tcgcgatcac ggtgatgtcc
     2041 ccgttgcgga acgcgaccac actgcgcccg tagcccttga gccactgcag ggccccgctc
     2101 gcgagcaggt gctcgcgccg cagacgaagg gcgagcgtgt agagctcgag cgtcgaaccg
     2161 ggcaccccga cctgggcgtc gcgcgcgaag cgcccccagt ccgtcggctg cggcaaccag
     2221 ctgtcaccgg tgtggttgaa gccgaatgcg gggcggtcgg cctcccaggg aatcggcacg
     2281 cggcatccgt cacgaccgta cttctcgccg tgggtgcgga accaggtcgg atcttgccgg
     2341 gccgcgtcgg gcagctccat gtcctccgga agcccgagct cttcgccctg gtagatgcag
     2401 ctgctgccgg gcagggccag catgagggat gtcgccgcgc gcgcccggcg caggccgatg
     2461 gcctcgttgg gcttgccggg ggacttcggt ccgatgccgg caccctgcgg actgtcggcc
     2521 gtgagggcga gccgcgaggc gtgccgcacg acgtcgtggt tcgacagcac ccaggtgctc
     2581 ggggcgccga cctcgccgaa cgccttgagc gattcggtga tgatgcgctt gagcgccggc
     2641 gccttccagt cggtgaacat gtagccgaag ttgaaggcct ggctcatctc gtcggggcgc
     2701 acccagagcg caaccttctc gagcgggtcg acccacgcct cggcgcagag cacgcggtcg
     2761 ccctcgtact cggcgagcac cttgtgccag ccgcgccaga tgtcgtgcac
//
LOCUS       DAOSOU010000252         2768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_36489_length_2768_cov_5.787689, whole genome shotgun sequence.
ACCESSION   DAOSOU010000252 DAOSOU010000000
VERSION     DAOSOU010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36489_length_2768_cov_5.787689"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLW15_10925"
     CDS             complement(<1..691)
                     /locus_tag="PLW15_10925"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HOT34947.1"
                     /translation="MSALILPAGRPATCVETGCFCEAVSGGIPTQIEASWSSLAFVLL
                     GVWAILQRGAPATRERTLLPLAGLTMIAIGVSSFIYHATLTFVGQWLDVFSMYLLGLL
                     IALGALWRAGRLGGRAAVALYVVLAIVLGVAQFLYPDARRILFALVLLPGVILELTPF
                     IAGRGPGSRRWVLASLATFVVAYVVWIIDQSALCNPRFWLQGHAVWHVLGAVAALLLT
                     VHCRRTPHPSSG"
     gene            complement(741..1046)
                     /locus_tag="PLW15_10930"
     CDS             complement(741..1046)
                     /locus_tag="PLW15_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOT34948.1"
                     /translation="MPPATHTFTTALWEMPTTAAAWVFVTVPTAASEVIKSTPRPPRP
                     GFGSLRVRATIGDTTWATSIFPDSKTGCYVLPVKKSVRVTEGIGLGDTVGVTVEVLE"
     gene            1161..1454
                     /locus_tag="PLW15_10935"
     CDS             1161..1454
                     /locus_tag="PLW15_10935"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HOT34949.1"
                     /translation="MTASRAIDRENKDARINVRLAPSQASLIRRAAEASSTSVSEFVI
                     GSAAVAAEQVLADRRWFTLDEESWRAFEELLERPAEYKPRLAELLAADDVFVD"
     gene            1447..1965
                     /locus_tag="PLW15_10940"
     CDS             1447..1965
                     /locus_tag="PLW15_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT34950.1"
                     /translation="MTDGTRFSRPRKLVASDPVREFRCGQPDLDEWIGRHALTNQQSG
                     MATVFVSRSGARIAGFYALATGAVEHTDATHRVTRGVPRHPIPVVLLTRLAVDEHHQG
                     AGLGGMLVRDALLRVDRAAEEIGVRALLVHAKDERARDYYLRLAEFESSPTDPLHLFL
                     LMKDLRRAIVGS"
     gene            2042..2521
                     /locus_tag="PLW15_10945"
     CDS             2042..2521
                     /locus_tag="PLW15_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT34951.1"
                     /translation="MPPSDASFERAYTQYLPAVSGYLFRRVERQHVEDLAADVFAIAW
                     RKRASVAEGEELPWLYRIAANVVANHRRKQATGTSLLAALRPADSAPSAEEIVVADAS
                     LAAAWRRLRPAEREVLALALVEDLPPTELAVALGVSVNAASIRVHRARTKLAALLAD"
     gene            2573..>2768
                     /locus_tag="PLW15_10950"
     CDS             2573..>2768
                     /locus_tag="PLW15_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34952.1"
                     /translation="MSSNHDDLSNRLRHSLDRGAAPELSPEMVTEAGIRTAPHLTTPG
                     RTLRIAGSAGLAIAAVTVGAL"
BASE COUNT          473 a         1006 c          916 g          373 t
ORIGIN      
        1 ggcccgaact ggggtggggc gtcctccggc agtgcaccgt gagcagcagc gcggcgaccg
       61 cccccagcac gtgccagacc gcatggccct gcagccagaa gcgcgggttg cacagggctg
      121 actggtcgat gatccacacc acgtacgcaa cgacgaaggt ggcgagcgag gcgagcaccc
      181 agcgacgtga ccccggtccg cggcccgcga tgaacggcgt cagctcgagg atcaccccgg
      241 gcagcagcac cagcgcgaag aggatgcggc gggcatccgg gtagaggaat tgagcgacgc
      301 cgagcacgat cgccagcacg acatagagcg cgaccgcggc tctgccgccg agccgcccgg
      361 cccgccacag tgcgccgagc gcgatgagca ggccgaggag gtacatgctg aagacgtcca
      421 gccactggcc gacgaacgtg agcgtcgcgt ggtagatgaa gctcgagacg ccgatcgcga
      481 tcatggtgag gccggcgagc ggcagcaggg tgcgctcccg ggtcgccggt gccccgcgct
      541 ggaggatggc ccacactccc agcagcacga acgccagcga actccagctg gcctcgatct
      601 gggtcgggat gccgccactc accgcctcgc agaagcaccc ggtctcgacg caggtcgccg
      661 gccgccccgc gggcaggatc agggcgctca cccaggacgc gacgccgacc gcccccgtgg
      721 ccgctaccgc gatgccggcc ctactccaga acctcaaccg tcacccccac cgtgtcgccc
      781 aacccgatgc cctcggtgac tcgcaccgac ttcttgacgg gcagcacgta gcatccggtc
      841 ttcgagtcgg gaaagatcga ggtcgcccag gtggtgtcac cgatcgtcgc gcgcacgcgg
      901 agcgagccga agcccggtcg cggcggtcgc ggcgtgctct tgatcacctc ggatgccgcg
      961 gtcggcaccg tcacgaacac ccacgccgcg gcggtggtgg gcatctccca cagcgcggtg
     1021 gtgaaggtgt gcgtggcggg tggcatgagg tgagcgtagt ggcgtggata acccaggacc
     1081 gacggaaccg ccgaggcgta gcatccgtgg accatctcag cgtgcctgta tgtacaatcg
     1141 acgtacagag aggactcatc atgaccgcct ccagggccat cgaccgagag aacaaggacg
     1201 cgcgcatcaa cgtgcggctc gcgccatcgc aggcgagcct gatccgtcgt gcggctgagg
     1261 ccagcagtac gagcgtgagc gagttcgtca tcggcagcgc cgcggtcgcc gccgaacagg
     1321 tgctcgccga tcggcgctgg ttcaccctcg acgaagagtc ctggcgcgca ttcgaggaac
     1381 tgctcgaacg ccccgcggag tacaagccac gactggccga actcctcgcc gccgacgacg
     1441 tcttcgttga ctgacggcac gcgcttcagc cgcccgagga aactcgtcgc gagcgacccc
     1501 gtgcgcgagt tccgatgcgg acaacccgac ctcgacgagt ggatcggccg ccacgcgctg
     1561 accaaccagc agtcgggcat ggcgacagtc ttcgtcagca ggtcaggagc gcgcatcgct
     1621 ggcttctacg cgctcgccac cggcgccgtc gagcacacgg atgccacgca ccgcgtcacc
     1681 agaggcgtgc cccgccaccc catcccggtc gtcctgctca cccggctagc ggtcgacgag
     1741 catcaccagg gcgccggact cggcggcatg ctcgtcaggg atgccctgct gcgcgtcgac
     1801 cgtgccgccg aggagatcgg ggtgcgagcc ctgttggtgc acgccaagga tgagcgagct
     1861 cgcgactact acctgcggct cgccgagttc gagtcgtcgc cgaccgaccc gctgcacttg
     1921 ttcctgctga tgaaggacct gcgcagggca atcgtcggca gctaggcgac gcatccccag
     1981 acgctcaact gccccaaaac gttgcgttgt gatccgacgc agctccggtc aggctggacg
     2041 catgccccca tccgacgcgt cgttcgagcg cgcctacact cagtacctcc ccgcggtgag
     2101 cggctacctg ttccggcggg tggagcggca gcacgttgaa gacctcgcgg ccgacgtgtt
     2161 cgcgatcgcc tggcgcaagc gcgccagcgt cgccgagggc gaggagctgc cctggctgta
     2221 ccgcatcgcg gcgaacgtcg tggccaatca ccggcgcaag caggccaccg gcacgtcgct
     2281 cctcgcggca ctgcggccgg ccgactcggc tccgtcggcc gaggagatcg tcgtggctga
     2341 cgcatccctc gctgcggcct ggcgtcggct tcgccccgcc gaacgcgagg ttctcgcgct
     2401 cgccctggtc gaggacctgc cgccgaccga actcgcggtg gcgctcggcg tgagcgtgaa
     2461 tgccgcgagc atccgggtgc atcgggcgcg cacgaaactc gcggcgctgc tcgccgactg
     2521 agaattttcg gatgctgctg aaagaatcgc ccactcgcga catttccccc atatgagcag
     2581 caatcacgac gacctcagca accgcctccg ccacagcctc gaccgcggag ccgcccccga
     2641 actctccccc gagatggtga ccgaagccgg catccgtacc gccccgcacc tcaccacccc
     2701 cggtcgcacc ctgcgcatcg caggcagcgc cgggctcgcg atcgccgccg tcacggtcgg
     2761 cgccctcg
//
LOCUS       DAOSOU010000253         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_36600_length_2762_cov_4.379387, whole genome shotgun sequence.
ACCESSION   DAOSOU010000253 DAOSOU010000000
VERSION     DAOSOU010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36600_length_2762_cov_4.379387"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PLW15_10955"
     CDS             complement(<1..1257)
                     /locus_tag="PLW15_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34953.1"
                     /translation="MTRSALRRSVSALLVAGLSLAGVALTASPASASSFEVTTTADAG
                     AGSLRDAIALANANAGDDTITFQAGLGDVIITSGPLLVTEGVTFDGPGSSELSIVRNG
                     SFSLFTVNIANTATALDVTFDGLDFIGNSISDTGLAIEFIAGDPEVDDITLSDTVFGG
                     FANTYEGGAVAVFGATGILTITNSTFAANSTPYPGGALYVGTLGDIVRISGSTFENNA
                     SYAAPDSAPEGGAIYANSAAGLELTDSTFTNNTTDGLGGAIAFDVIGVNSLIDLVTFS
                     NNSAGSVDNPLNYGGSIYLGTIVTGAQLVISRTAIIGSTLTEGFVETYGAGLFVNDID
                     GSLTIDSSTFTGGEFISDETPPGGGLSIALCSINEGGELLVIDSTFDEHSTSGAYAIH
                     ACDQSGELTIAFPTLVAPGVLFVGDNA"
     gene            complement(1356..>2762)
                     /locus_tag="PLW15_10960"
     CDS             complement(1356..>2762)
                     /locus_tag="PLW15_10960"
                     /inference="COORDINATES: protein motif:HMM:NF037006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT34954.1"
                     /translation="LDLLEDPKTLDDPVVAATDQEFALQAFKSLPERWQSVLWYSAVE
                     GMGPTEVAGYLGMSPNAAAVLAHRARAGLRTAWVQAHLNDATLSDDCRWTAGRLGRHA
                     SGALSARERARVTDHLATCKACRARARELDQLTSKLAVLLVPALLGLGASLRDSNGPI
                     TAAAIPPSELAGTALAPAHAWLAGIAVAVVTATAFITASAPPEATVAETAAAPIQTPR
                     PTTDSPAEDLLPTLGSPADPSAGSSADDVPTGSATEPLGSATPPASTPPIPVAPPPAA
                     PAVPVVTTAIDPYAVVRPSLEGTALPGAQIVVTDELGATIATVVADAAGAWSTGQLAS
                     LSPAAAAIEVRQIDDQGRASAVTTVDLAPMPLILSTLPNGATYADSGFELVVVGWPGS
                     TVQATFSPATSPDWPDYLPAESTYPDTVVAIDWQGLGSIWIDSTIPGGHRVDLRYTDG
                     VRSSTGVSSYNFVVWLPD"
BASE COUNT          445 a          830 c         1027 g          460 t
ORIGIN      
        1 cgcgttgtcc ccgacgaaca gcacgcccgg ggcaacgagg gtggggaagg cgatcgtcag
       61 ctcgccgctc tggtcgcagg cgtggatcgc gtaggcaccg ctcgtggagt gctcgtcgaa
      121 ggtcgagtcg atcacgagca gttcgccgcc ctcgttgatg ctgcacagcg cgatgctgag
      181 gccaccgccg ggcggcgtct cgtcgctgat gaactccccg cccgtgaagg ttgacgagtc
      241 gatggtgagg gagccgtcga tgtcgttgac gaagaggcca gctccgtagg tctcgacgaa
      301 gccttcggta agggtgctgc cgatgatcgc ggtacgcgag atcaccagtt gtgccccggt
      361 gacgatagtg ccgagataga tgctgccgcc gtagttgagg gggttgtcga ccgacccggc
      421 cgagttgttc gagaacgtga ccaggtcgat gaggctgttg accccgatga cgtcgaaggc
      481 gatggcaccg cccagaccgt cggtggtgtt gttcgtgaac gtcgagtcgg tgagctccag
      541 tccggcggcc gaattggcgt agatggcgcc accctccggc gcgctgtcgg gtgccgcgta
      601 cgatgcgttg ttctcgaagg tagacccgga gatgcgcacg atgtcgccga gtgtgcccac
      661 gtacagcgca ccacccggat agggagtgct gttggccgcg aaggtcgagt tggtgatggt
      721 gaggatgccc gtggccccga agaccgcgac ggcacctccc tcgtacgtgt tcgcgaatcc
      781 gccgaagacg gtgtcgctca aggtgatgtc atccacttcg ggatcaccgg cgatgaactc
      841 gatggcgagc cccgtgtcgc tgatggagtt gccgatgaag tcgagaccgt cgaaggtcac
      901 atcgagcgcg gtcgcggtgt tcgcgatgtt cacggtgaag agtgagaacg acccgttgcg
      961 cacgatggac aactcggaag agcccgggcc gtcgaaggtg actccttcgg tgaccagcag
     1021 cgggccgctc gtgatgatga cgtcgccgag cccggcctgg aaggtgatgg tgtcgtcacc
     1081 ggcgttcgcg ttggccagtg cgatcgcatc acgcaggctg ccggcgcccg cgtccgcggt
     1141 ggtggtcacc tcgaagctgc tcgcgctggc ggggctggcg gtcagcgcga ctcctgccag
     1201 cgagaggccc gcgacgagaa gggccgacac ggatcgacga agtgctgatc gtgtcacggt
     1261 catccaatct gaaacgggct cgtgacctca tcgtagcgaa cgggggtcgc gaagccgagc
     1321 ccccttcacg cggcggagat tcagacgccg gagaatcagt ccggcagcca gacgacgaag
     1381 ttgtaagaac tgacgcctgt cgagctgcgc actccgtcgg tgtaccgcag gtcgaccctg
     1441 tgaccaccgg ggatcgtact gtcgatccag atcgacccca gaccctgcca gtcgatcgcg
     1501 accacggtgt cggggtaggt cgactcagca ggcaagtaat cgggccagtc aggcgacgtg
     1561 gcggggctga acgtggcctg cacagtgcta ccgggccagc cgacgacgac gagttcgaat
     1621 cccgagtccg cgtacgtcgc tccgttgggc agcgtgctca ggatgagcgg catgggggcg
     1681 aggtcgaccg tcgtgactgc cgatgctctt ccctggtcgt cgatctgccg gacctcgatg
     1741 gcggcagcgg cgggggagag ggatgcgagc tggcctgtcg accaggcgcc tgcggcatcc
     1801 gccacaacgg ttgcgatcgt ggcaccgagt tcatcggtca ccacgatctg cgcgccgggc
     1861 agggcggtgc cttcgagcga cggtcgaacg acggcgtacg ggtctatcgc ggtagtgacg
     1921 acgggcacgg ccggcgccgc gggcgggggc gcaacgggaa ttggcggcgt ggacgcgggc
     1981 ggggtggcgc tgccgagcgg ttctgttgcc gagcccgtgg gcacgtcgtc tgctgaactg
     2041 ccggcagacg gatctgcggg cgagcccagg gtcggcagca aatcttcggc cggcgaatcg
     2101 gttgtcgggc ggggagtctg gatgggtgct gccgcggtct ctgcgaccgt cgcctccggc
     2161 ggagccgatg ccgtgatgaa cgcggtcgcg gtgacgaccg cgaccgcgat gccggccaac
     2221 caggcgtgtg ctggtgcgag tgctgtgccg gcgagttcgc tcggcggaat ggcagcagcg
     2281 gtgatggggc cgttggagtc gcgaaggctt gcacccagac cgaggagcgc gggtacgagg
     2341 agcaccgcga gcttggacgt gagctggtcg agttctcggg ctctggcgcg gcacgccttg
     2401 caggtggcga gatggtcggt gaccctcgcc cgctcgcgag cactaagcgc cccgctcgcg
     2461 tgtctaccga ggcggcctgc ggtccagcgg cagtcgtcgg agagtgttgc gtcattgagg
     2521 tgcgcctgga cccaggccgt gcgcaggccc gccctggccc ggtgcgcgag aaccgcggcg
     2581 gcgttgggtg acatgccgag gtagcctgct acctcggtcg gccccatgcc ctcgacggcg
     2641 ctgtaccaga gcaccgactg ccaccgctcg gggagcgact tgaacgcctg gagggcgaac
     2701 tcctgatcgg tggccgcgac caccgggtca tcgagcgtct tgggatcttc gagaaggtcg
     2761 ag
//
LOCUS       DAOSOU010000254         2740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_37008_length_2740_cov_3.903166, whole genome shotgun sequence.
ACCESSION   DAOSOU010000254 DAOSOU010000000
VERSION     DAOSOU010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37008_length_2740_cov_3.903166"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..266)
                     /locus_tag="PLW15_10965"
     CDS             complement(18..266)
                     /locus_tag="PLW15_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HOT34955.1"
                     /translation="MSIGSGIALIVIGAIITFAINIEISWIDLDIVGSILMGAGLVIF
                     ILGIVGMTRKRQVVETVRVAADPATGQQVTERKTSAPR"
     gene            386..1426
                     /locus_tag="PLW15_10970"
     CDS             386..1426
                     /locus_tag="PLW15_10970"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOT34956.1"
                     /translation="MSSTLNLAVISGDGIGPEVIAEALKVLKATGIDVTTTDFPFGAS
                     HFLATGNILTEEDLAALATHDAILLGAVGGDPRDPRLAGGIIERGLLLKLRFAFHHYV
                     NLRPTVLLPGVPSPLKDPGTVNFVVVREGTEGPYVGNGGTIRQGTAHEIATEVSINTA
                     FGVERLVRFAFTEAEARPRKHVTLVHKTNVLTHAGGLWQRTVDRVAAEHPGVTVDYQH
                     IDAATIHMVTDPARFDVIVTDNLFGDIITDLAAAISGGIGLAASGNINPDGTFPSMFE
                     PVHGSAPDIAGQQLADPTAAILSVALLLTHTGHPDAAARVTAAVEADLAARGTAKRST
                     AQIGDDIARRVE"
     gene            1531..2646
                     /locus_tag="PLW15_10975"
     CDS             1531..2646
                     /locus_tag="PLW15_10975"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOT34957.1"
                     /translation="MKNLLAPRTLEFSVTPNDAARSAAEHADIIAEPGFGKHFTDHMV
                     DICWSEQGGWHRPRVQPYGPIQLDPAAAVLHYAQEVFEGLKAYRHADGSIWTFRPEMN
                     AERLKRSARRLALPELPTEYFIESLRQLIAVDARWVPSAADTSLYLRPFMFAKEAFLG
                     VRPAKKVNYYVIASPAGAYFSGGVAPVSIWISTDYARAGKGGMGAAKTGGNYASSLVA
                     QQEAYDHGCAQVLFLDSEKGEYLEELGGMNVVFVYKDGRVVTPESDSILEGITRDSVL
                     QLAKDRGLKVEQRKVSITEIREAVASGEIVEMFACGTAAVIVPISQFKADGYTIGEAD
                     APAGELTMSLREELTDIQYGRKHDRHGWMTRLDVVPE"
BASE COUNT          489 a          959 c          852 g          440 t
ORIGIN      
        1 cgcggcagcg cttcgtgtca gcgcggagcg gacgtcttgc gctcggtgac ctgctgtccg
       61 gtcgcggggt ctgcagcgac acgaaccgtc tccacgacct ggcgcttgcg cgtcattcca
      121 acgatgccga ggatgaagat caccaggcca gcgcccatca ggatgcttcc cacgatgtcc
      181 agatcgatcc aactgatctc gatgttgatg gcgaacgtga tgatcgcccc gatcacgatg
      241 agggcgatgc cacttccaat actcatggca acaggctagc gattccgggg ccgagccatg
      301 gtgaacactc ggttagccgc cgagaggcga ggcccgcacg ggtagaagcg ctcttccgga
      361 ccatttcgac aagtactgta aaagcatgtc cagcaccctc aatctcgccg tcatctccgg
      421 tgacggaatc ggtcccgaag tgatcgccga agccctcaaa gtactgaagg cgaccggtat
      481 cgacgtaacc acgaccgact tcccattcgg cgccagccac ttcctggcga ccggcaacat
      541 cctgaccgag gaggacctcg ccgccctcgc gacgcacgac gccatattgc tgggcgcggt
      601 cggcggcgac ccgcgcgacc cgcggctggc ggggggcatc atcgagcgcg gactcctgct
      661 caagctgcgg tttgccttcc accactacgt gaacctgcgc cccacggtgc tgctgcccgg
      721 tgttccttcg ccgctcaagg atcccggcac ggtgaacttc gtcgtggtgc gagaaggcac
      781 cgaaggtccg tacgtcggca acggcggcac gatccgccag ggcaccgcgc acgagatcgc
      841 caccgaggtg agcatcaaca ccgccttcgg cgtggagcgc ctggtgcgct tcgccttcac
      901 cgaagccgag gctcggccgc gcaagcacgt gacgctcgtg cacaagacca acgtgctgac
      961 ccacgcgggc ggtctgtggc agcgcaccgt cgaccgggtc gcggccgagc atcccggtgt
     1021 cactgtcgac taccagcaca tcgatgccgc gacgatccat atggtcaccg atcctgctag
     1081 gttcgatgtg atcgtcacag acaacctctt cggcgacatc atcaccgatc ttgcagccgc
     1141 tatcagcggc ggcatcggac tggcggcctc gggcaacatc aatccggatg gcaccttccc
     1201 ctcgatgttc gagcccgtgc acggttcggc cccggacatc gccggtcagc aactcgccga
     1261 ccccactgcc gcgatcctgt ccgttgcact cctgttgact cacacgggtc acccggatgc
     1321 cgcggctcgg gtcaccgcgg ccgtggaggc cgaccttgcc gcccggggga ccgccaagcg
     1381 cagcacagcg cagatcggcg acgacatcgc caggcgggtt gagtagcgcg aagcgcgtat
     1441 cgaaaccggc gctgtcaggt ttcgatacgc ccctgcgggg ctactcaacc ggcgtagcat
     1501 ccatttagct cctctccact aaggaacacg atgaagaacc tcctcgcccc gcgcaccctc
     1561 gagttctcgg tgacgcccaa cgacgccgcc cgctccgccg ccgagcacgc cgacatcatc
     1621 gccgagcccg gcttcggcaa gcacttcacc gaccacatgg tcgacatctg ctggagcgag
     1681 cagggcggat ggcaccgtcc ccgggtgcag ccgtacgggc cgatccagct cgacccggcc
     1741 gccgcggtgc tgcactacgc gcaggaggtc ttcgagggcc tcaaggccta ccgtcacgcc
     1801 gatggctcga tctggacgtt ccgccccgag atgaacgccg agcggctcaa gcgctccgcc
     1861 cgccgtctgg cactgcccga gctgccgacc gagtacttca tcgagtcgct gcgccagctg
     1921 atcgccgtcg acgcccgctg ggtgccctcg gcggctgaca cgagcctgta cctgcgcccc
     1981 ttcatgttcg ccaaggaggc gttcctcggc gtgcgcccgg ccaagaaggt caactactac
     2041 gtgatcgcga gccctgcggg cgcctacttc agcggtggcg tggcgccggt gtcgatctgg
     2101 atctccaccg actacgcccg cgcgggcaag ggcggaatgg gtgcggccaa gaccggcggc
     2161 aactacgcgt catccctcgt cgcccagcag gaggcctacg accacgggtg tgcccaggtg
     2221 ctgttcctcg actccgagaa gggtgagtac ctcgaggagc tcggcggcat gaacgtcgtg
     2281 ttcgtctaca aggacggccg cgtggtcact cccgagtccg acagcatcct cgagggcatc
     2341 acgcgcgact cggtgctgca gctggccaag gaccgcggtc tgaaggtgga gcagcgcaag
     2401 gtgagcatca ccgagatccg tgaggctgtg gcatccggcg agatcgtcga gatgttcgcc
     2461 tgcggtaccg ctgcagtgat cgtgccgatc agccagttca aggccgacgg gtacacgatc
     2521 ggtgaggcgg atgccccggc cggcgaactg acgatgtcgc tgcgcgagga gctcaccgac
     2581 atccagtacg ggcgcaagca cgatcgccac ggctggatga cgcggctcga cgtcgtgccc
     2641 gagtagtcct ccgctggttg agtagctcgc gcagcgagcg tatcgaaacc gcctacgaga
     2701 cccgccccac aagccccgca acagcatccg ccatgctctc
//
LOCUS       DAOSOU010000255         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_37195_length_2729_cov_7.036649, whole genome shotgun sequence.
ACCESSION   DAOSOU010000255 DAOSOU010000000
VERSION     DAOSOU010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37195_length_2729_cov_7.036649"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLW15_10980"
     CDS             complement(<1..206)
                     /locus_tag="PLW15_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34958.1"
                     /translation="MTASPDTNAEMSAYWDGERGEAWVARADQFDRQLEIYGLRAVSS
                     AAPQPGETVIDIGCGAGATSFEAS"
     gene            320..1468
                     /locus_tag="PLW15_10985"
     CDS             320..1468
                     /locus_tag="PLW15_10985"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT34959.1"
                     /translation="MAEAYIIDTVRTPVGKRGGSLSQIHPADLGAHTLKALVERTGID
                     PGAVEDVVMGCLDTIGPQAGDVGRSAWLAAGLPEHVPGVTIDRQCGSSQQAVHFAAQG
                     VMSGTQDLVIAAGLQNMSMIPISSAMTVAEPMGFTDPFTGSEGWVARYGTQEVSQFRG
                     AEMIAEKWDISRDDMEAFAIESHTRAIRAREEGRFDNEIVPLNGLTFDEGPREPNVEK
                     IRSLKTLVEGGRVTAAVASQISDASAAMLIASEQAVKDHGLKPRARIHHLSVRADDPI
                     FMLSAPIPATRYALDKAGMTLDDIDLIEINEAFASVVLAWQKELDVDLAKVNVNGGAI
                     ALGHPLGATGVRLMTTMLNELERTGGRYGLQTMCEGGGQANVTIIERL"
     gene            1565..>2729
                     /locus_tag="PLW15_10990"
     CDS             1565..>2729
                     /locus_tag="PLW15_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT34960.1"
                     /translation="MDFEDSAEEAAFRAEVREFLGAHATPKAGDESDWSRNGASVDPV
                     VAEDYRRRCRDWQRTLYDNGWAGIAWPKAFGGRGGNAAQQIIFNQELAKFDATSGFLT
                     AALALVGPTLMAHGTPEQQQRYLAPLLSGEESWCQLFSEPEAGSDLATLGTRAVLDGD
                     EWIVNGQKVWNSSAQHADFGILICRTDPSVPKHNGITYFIVDMRTPGIEVRPLVQAQG
                     VAHFNEVFFSDVRIPAANVVGTVNDGWKVTKTTMRSESSMISGAGQATTFVNVLATVR
                     RCGKDTDPVVRQELAKVWSNEQILRYLSMRTQTAVMTGRRDLAIHGSLLKNFFTRSFT
                     HRATLALDCEGAEGMLSGSDAEGDGFWQYQCINQFASKIGGGTEEVHRNNLAEQ"
BASE COUNT          469 a          973 c          882 g          405 t
ORIGIN      
        1 tgggaggcct cgaagctcgt tgccccggcc ccgcacccga tgtcgatgac ggtctcgccg
       61 ggttggggcg cggccgacga gacggcgcgg aggccgtaga tctcgagctg tcgatcgaac
      121 tggtcggcac gggcgaccca ggcctcgccg cgttcgccgt cccagtaagc ggacatctcg
      181 gcgttcgtgt caggggatgc ggtcatggag cgagcttccc acgcttcgac ccgccgtcgc
      241 cggtcacgta accttggggc accaaacgag aacgcgttct agaactgctt caggacagcg
      301 tcgggcaaca ggagacacca tggccgaggc ctacatcatc gacaccgtgc gcacccccgt
      361 cggcaagcgc ggcggcagcc tgtcgcaaat ccaccccgcc gacctcggcg ctcacaccct
      421 caaggccctc gtcgagcgca ccggcatcga tcccggcgcg gtcgaggacg tggtcatggg
      481 ctgcctcgac acgatcggcc cacaggccgg cgatgtcggc cgttcggcct ggctcgccgc
      541 cggccttccc gagcacgttc ccggcgtcac gatcgaccgg cagtgcggtt cgtcccagca
      601 ggcggtgcac ttcgcagcgc agggtgtcat gagtggcacg caggacctcg tgatcgccgc
      661 cggcctccag aacatgtcga tgatcccgat cagctccgcc atgaccgtgg ccgagcccat
      721 gggcttcacc gatccgttca ccggttcgga aggttgggtc gcgcggtacg gcacccaaga
      781 ggtgagccag ttccgcggcg ccgagatgat cgccgagaag tgggacatct cccgcgacga
      841 catggaagcg ttcgcgatcg agagccacac acgcgccatc cgggcgcgtg aggagggccg
      901 attcgacaac gagatcgtgc cgctcaacgg cctcaccttc gacgaggggc cccgcgagcc
      961 caacgtcgag aagatccgct cgctcaagac cctcgtcgag gggggccgcg tcaccgccgc
     1021 cgttgcgtcg cagatctccg acgcatcggc cgcgatgctc atcgcgtccg agcaggcggt
     1081 gaaggaccac ggcttgaagc cccgggcccg catccaccac ctctcggtcc gcgccgacga
     1141 tccgatcttc atgctcagcg ccccgatccc ggccacccgt tacgcgctcg acaaggccgg
     1201 catgaccctc gacgacatcg acctcatcga gatcaacgag gcgttcgcct cggtcgtcct
     1261 cgcctggcag aaggagctcg acgtcgatct cgccaaggtc aacgtcaacg gtggggccat
     1321 cgcactcggc cacccgctcg gcgccaccgg cgtccgcctc atgaccacca tgctcaacga
     1381 gctcgagcgc accggcggcc ggtacggcct gcagacgatg tgcgagggcg gcggccaggc
     1441 caacgtgacg atcatcgagc gtctgtagga gcctggtgat gcgcattgtg actgtcctga
     1501 tggggacctg ccgcctggcg gcgcacatcc cggccatcga gcgcctgtaa gcgtcactcg
     1561 ctccatggac ttcgaggact ccgcagaaga agccgcgttc cgcgccgagg tgcgcgagtt
     1621 cctcggcgcg cacgccacac cgaaggccgg cgacgagagc gactggtcgc gcaacggcgc
     1681 gtcggtcgat cccgtcgtcg ccgaggacta ccgccggcgc tgccgcgact ggcagcgcac
     1741 gctgtacgac aacggttggg ccggcatcgc ctggcccaag gcgttcggcg ggcgcggtgg
     1801 caacgcggcg cagcagatca tcttcaacca ggagctcgcg aagttcgacg ccacgagcgg
     1861 gttcctcacc gctgcgctcg cgctcgtcgg cccgacgctc atggcccacg gcacgcccga
     1921 gcagcagcag cgctacctgg caccgctgct cagcggtgag gagtcgtggt gccagctgtt
     1981 cagcgagccc gaggccggga gtgaccttgc caccctcggc acccgtgccg tcctcgacgg
     2041 cgacgaatgg atcgtcaacg ggcagaaggt gtggaactcc tccgcgcagc acgccgactt
     2101 cggcatcctg atctgccgca ccgacccgtc ggtgcccaaa cacaacggca tcacgtactt
     2161 catcgtcgac atgcgcaccc ccggcatcga ggtgcgcccg ctcgtgcagg cgcagggagt
     2221 cgcccacttc aacgaggtgt tcttcagcga cgtccgcatc ccagccgcca acgtggtcgg
     2281 tacggtcaac gacgggtgga aggtcaccaa gaccaccatg cgcagcgagt cgtcgatgat
     2341 ctcgggcgcc gggcaggcca ccacgttcgt caacgtgctc gccacggttc gtcgctgcgg
     2401 caaggacacc gatccggtgg tccgccagga gctggcgaag gtctggtcca acgaacagat
     2461 cctgcgttac ctgtccatgc ggacgcagac cgccgtcatg accggtcgtc gggatctcgc
     2521 gatccacggc agcctgctca agaacttctt cacccgctcg ttcacgcacc gggccacgct
     2581 ggcgctcgac tgcgagggtg ccgaaggcat gctctcaggt tccgacgccg agggcgacgg
     2641 gttctggcag taccagtgca tcaaccagtt cgcctccaag atcggcggcg gcaccgagga
     2701 ggtgcaccgc aacaacctcg cggagcagg
//
LOCUS       DAOSOU010000256         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_37219_length_2728_cov_6.281706, whole genome shotgun sequence.
ACCESSION   DAOSOU010000256 DAOSOU010000000
VERSION     DAOSOU010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37219_length_2728_cov_6.281706"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..652)
                     /locus_tag="PLW15_10995"
     CDS             complement(95..652)
                     /locus_tag="PLW15_10995"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT34961.1"
                     /translation="MSLPFSFDEPLVTERLSLRLMTEDDVDAVHEWMSDPEVVRYQLY
                     DVRDRATVAEKVAEYALASRLESNGDYVQPAIVVDGEVVGAIYFTLSSVDDLTAEIGW
                     VLGRAHQGKGYAFEAASAMLSVAFGEMGLHRVYAELDPRNAASVALCLRLGMRHEAHF
                     VEHMMFKGEWADTGIYGLLAREWKP"
     gene            758..1066
                     /locus_tag="PLW15_11000"
     CDS             758..1066
                     /locus_tag="PLW15_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOT34962.1"
                     /translation="MKGKILFVVGLGVGYVLGTRAGREKYDQMKSAALKLWNDPRVQK
                     RVDDAGDFVKDKAPEVAEFLADGAKKVVSQVSGSKAPETKAPAKPATRTSTSKSTPAK
                     "
     assembly_gap    1246..1345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1558..2310
                     /locus_tag="PLW15_11005"
     CDS             1558..2310
                     /locus_tag="PLW15_11005"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOT34963.1"
                     /translation="MAAKPLGGLRVLVPRGGKWGDGIAATLRTLGATPVIAPMINFAS
                     ADDGAALANALHELQDGQFDWIVITSATTVDVFVGHRVSIPSTTRIAAVGETTGAALQ
                     LAGYRVDFTPSDNSARGLVAQWGTSEISGRVLVPQSDVAEPTLVSGLGGLGLDVQFVS
                     AYRTVGVPVSDAVRADVASGRIAAILVSSGSVARQIAAQLAPLPEGTVVACIGPRTAF
                     DARAAGLAVDVIAEERSADSLVAALLEHVVQP"
     gene            complement(2307..2699)
                     /locus_tag="PLW15_11010"
     CDS             complement(2307..2699)
                     /locus_tag="PLW15_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HOT34964.1"
                     /translation="MLVAGSVFVSIAALIHVYIFVLESLRWSRPATWKTFGLKSQADA
                     DVVKPMAFNQGFYNLFLALGVVAGLGLIATSHTAAGLAIALFAAGSMVAAALVLIISS
                     PKLARAAAIQGIAPLVGVVLLGLALLVG"
BASE COUNT          449 a          891 c          877 g          411 t
ORIGIN      
        1 gcccagctgc cctcgaagtg ctgagggccg aggggttcca gccgcagacc gggggtctcg
       61 aggctcacct gatcgcggaa gtagtcgacg gggatcatgg tttccattct cgggccagca
      121 ggccgtagat gcccgtgtcg gcccactcac ccttgaacat catgtgctcg acgaagtggg
      181 cctcgtgccg cattcccagc ctcagacaca gggcgacgga tgccgcgttc cgtgggtcga
      241 gctccgcata cacccggtgc agtcccatct cgccgaatgc taccgaaagc atcgcgctgg
      301 ccgcttcgaa cgcgtacccc ttgccctggt gcgcccgacc caggacccat ccgatctcag
      361 cggtgaggtc gtcgacgctg gagagtgtga agtagatcgc tccgaccact tcgccgtcga
      421 cgacgatcgc gggctgcaca tagtcgccgt tgctctccag tcggctcgcg agggcgtact
      481 cggcgacctt ctcggcgacg gtggcgcggt cgcgcacgtc gtagagctgg tagcgcacga
      541 cctcggggtc cgacatccac tcgtgcaccg catccacgtc gtcctcggtc atgagccgca
      601 ggctgagacg ctcggtcacc aagggttcat cgaaggaaaa cggaagtgac attggcgtag
      661 cgtacattcc ctgtgaggca tggtctaccg ggcgacagca tctgcgggcg cggctagggt
      721 tgggagcacc acacactttc agggaacgga gcaccgcatg aagggcaaga tcctcttcgt
      781 cgtagggctg ggcgtaggtt acgtgctcgg cacccgcgcg ggtcgcgaga agtacgacca
      841 gatgaagtcc gcagcgctca agctgtggaa cgacccacgg gtgcagaagc gtgtcgacga
      901 cgcgggtgat ttcgtgaagg acaaggcgcc cgaggttgcc gagttcctcg ccgacggtgc
      961 gaagaaggtc gtatcgcagg tgtcgggctc caaggcaccg gaaacgaagg ctcccgcgaa
     1021 gcccgcgacc cgtacgtcca cctcgaagtc caccccagcg aagtagtcgg cgatgaccga
     1081 cacgtcgcgc ggcacacccc ggcggtccct gttcgccctc atcggcgaca tcccgcgcct
     1141 gctcatggac ctcgtgcgct ccgagatcga ggggctccag aaggagcttc tcgacaaggt
     1201 caagcacgcg ggcgtcggta tcggcctgat cgcgacggcc ctctcnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnaacgt gcccaagcag gtcggcgagc tgaccacccg
     1381 cgtgcgtgcc tcctacgagc gcaacccgct gccgtggatc atcggcgcag tgggtgccgc
     1441 ggtggcggtc gcggcggtgg tggcttgggc catcctgagc gacgacgact gatcgccgca
     1501 tactgatcgc cacccctcga ttcgtcagcc cgaccttcca caggggacga taggcacatg
     1561 gcagccaaac cactgggcgg cctccgcgta ctggttcccc gcggcgggaa gtggggggac
     1621 ggcatcgcgg cgaccctccg aaccctcggt gcgacccccg tcatcgcacc gatgatcaat
     1681 ttcgcgagcg cggacgacgg cgcagctctc gcgaatgcac tccacgaact ccaggacggt
     1741 cagttcgact ggatcgtcat caccagcgcg acgaccgtcg acgtgttcgt cgggcaccgc
     1801 gtgagcatcc cgtcgacgac ccgcatcgct gccgtcggtg agacgaccgg tgccgcactg
     1861 cagctggcgg gctatcgagt cgacttcacg ccgagcgaca actccgcgcg gggcctcgtc
     1921 gcccagtggg gcacctccga gatctccggc cgcgtgctgg tgccgcagtc cgacgttgcc
     1981 gagcccacgc tcgtgtccgg gctcggcggg ctcggcctcg atgtgcagtt cgtgtcggcg
     2041 tatcgcacgg tcggggttcc ggtctccgac gccgtgcggg cggatgtcgc atccggtcgc
     2101 atcgccgcga tcctggtcag ctcgggctca gtggcccgtc agatcgcggc ccagctcgca
     2161 ccgttgcccg agggaaccgt tgtcgcatgc atcggcccgc gcaccgcctt cgacgcgcgc
     2221 gccgcaggac tcgccgtcga cgtcattgcc gaggagcggt cggccgattc gctcgtggcc
     2281 gcgcttctcg agcacgtcgt gcagccctag ccgacgagca gggcgaggcc cagcagcacg
     2341 acgccgacga gaggggcgat gccctggatc gcggccgccc gcgcgagctt gggcgacgag
     2401 atgatcagca ccagagcggc agcgaccatt gagcccgccg cgaagagtgc gatcgcgagt
     2461 ccggcagcgg tgtgcgaagt cgcgatcagc ccgagtccgg ccacaacgcc cagggcgagg
     2521 aacaggttgt agaacccctg gttgaacgcc atgggcttga ccacgtcggc gtcggcctgc
     2581 gacttcagcc cgaaggtctt ccacgtcgcg ggccgcgacc agcgcaggct ctccagaacg
     2641 aagatgtaga cgtggatgag ggcggcgatc gacacgaaga cagacccggc cacgagcagc
     2701 gcagtgttca tgcccgccag catgccac
//
LOCUS       DAOSOU010000257         2646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_38723_length_2646_cov_5.097646, whole genome shotgun sequence.
ACCESSION   DAOSOU010000257 DAOSOU010000000
VERSION     DAOSOU010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38723_length_2646_cov_5.097646"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..377)
                     /locus_tag="PLW15_11015"
     tRNA            complement(305..377)
                     /locus_tag="PLW15_11015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Lys,seq:ttt)
     gene            404..1033
                     /locus_tag="PLW15_11020"
     CDS             404..1033
                     /locus_tag="PLW15_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT34965.1"
                     /translation="MADDFHKPTQYSGDKFDEYEGGEDPAQILRVAHDTAHALVNRAR
                     ETDDPAVLERLVAYTDSHGIDALAELWARSSPRSLPGALWRIYLVRVVIRKDSDGTAL
                     LFQRGTEVLATIDAAVAGAPQPAGPDEITELADQILRGLFRGDFAIALERAAAFCRIL
                     SAGATSIADDADPTNPERATELTTRANRLTLTAEELTGSARLYRIGSLD"
     gene            complement(1412..2200)
                     /locus_tag="PLW15_11025"
     CDS             complement(1412..2200)
                     /locus_tag="PLW15_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOT34966.1"
                     /translation="MTSDKELGNLSGSYALNGLDAADLAEFEAHLGQSPATRDEVTEL
                     TDTAVLLGLATRPVQPSAGLKASIMDQLDAHPQLPVAASSAAGASSASVATAAFESPA
                     GRKAQARWFTRPVTALAAVAAAIALFVGGGVVTTSLAQNSFAQQQADQLAAINSADDA
                     QRASVDLADGGTATLVWSNTLLSSALIVDGLAPLPADRTYELWYINDAGARPAGTFGV
                     AGSGSTWRVLDGTMKAGDTVGVTVEPRGGSDAPTTDPVVVIASA"
     gene            complement(2197..>2646)
                     /gene="sigK"
                     /locus_tag="PLW15_11030"
     CDS             complement(2197..>2646)
                     /gene="sigK"
                     /locus_tag="PLW15_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HOT34967.1"
                     /translation="LVTRLLRDPAQSEEVTQEIFLEIWQTSSRFDPNKGGASTWILTM
                     THRRAVDRVRASQASRDRDVKVGIRDLDADYDHVAESVEVRIEHERVEKAMARLTDMQ
                     RQAITLSYYGGYSNSEVAEILSVPIGTIKTRIRDGMIRLRDEMGVAS"
BASE COUNT          411 a          928 c          894 g          413 t
ORIGIN      
        1 cctgtgtccg gaagacggca aagtgtcgga accagcgcga gggggacgac ggctgcgcgc
       61 accgacgcgt gggcccggcg gggctcgacg ccccatcgtt cccctactga gccgccgaca
      121 catcgctgcg cgatgcatcg gtctgctcag cgggggccca ggccgccacg tcgctggtta
      181 tggcgtgagt gcgtggcatc ccggatcgcg gcgacgcgtc ggaaccggcg cgatggggac
      241 cccggctgag ttcttctact ccgggaagac gtgccgtcgc cgcccggtgg gcgacaccga
      301 cgcgtgggcc cggcggggct cgaacccgcg acccgcggat taaaagtccg atgctctacc
      361 aactgagcta caggccctac agatcaagcg taccgttgag ttgatggccg acgatttcca
      421 caaacccacc cagtactcgg gcgacaagtt cgacgagtac gagggcggcg aagaccccgc
      481 ccagattctg cgcgtcgccc acgacaccgc ccacgcgctc gtcaaccgcg cgcgcgagac
      541 cgacgacccg gccgtgctcg agcggctcgt cgcctacacc gactcgcacg gaatcgacgc
      601 gctcgccgag ctgtgggcgc gctccagccc gcgcagcctg ccgggcgcgc tgtggcgcat
      661 ctacctggtg cgggtcgtca tccgcaagga ctcggatggc acggccctcc tgttccagcg
      721 cggcaccgag gtgctggcga ccatcgacgc cgccgtggcc ggcgcacccc aaccggccgg
      781 acccgacgag atcaccgaac tcgccgacca gatcctgcgc ggactgttcc gcggcgactt
      841 cgccattgcg ctggagcgcg ccgccgcgtt ctgccgcatc ctcagtgccg gggccaccag
      901 catcgcggat gacgcggacc ccaccaaccc cgaacgtgcc accgaactga ccactcgagc
      961 caaccgcctc accctcaccg ccgaggagtt gacgggttcg gcccgcctct accggatcgg
     1021 cagcctggac taggtcggcc cgcgctcgcg cgcacgctgc ggacatttcg gcctgccgta
     1081 gcgcccgcgc gtgtccgcaa tatgcgcgcg aaccggcgtg cgtgtccgca gcatgcgcgc
     1141 gagcgggttg agcgcgcggc atccggtctc gacaagctcg accaccggct ccaccaccgc
     1201 ttcgctttgg taaactagtt cggccgggcc gcagtaaccc cgggctccac taatagccgc
     1261 ttcgagcggc cttgcgccga gaggcgttct gcggcccggc attacttctg cccgctggtt
     1321 gagtagcggc caagggccgc gtatcgaaac catgcaaccg cgatgttggt ttcgatacgc
     1381 cgactccgtc ggctactcaa ccagcggggt gctacgcgga agcgatcacg accaccgggt
     1441 cggtcgtcgg tgcgtcagac ccgccgcgcg gctccacggt gacgccgacg gtgtcgccgg
     1501 ccttcatcgt tccgtcgagc acgcgccagg tgctgccgct gccggcgacc ccgaaggtgc
     1561 cggcggggcg tgcgcccgcg tcgttgatgt accagagctc gtaggtgcgg tcggctggca
     1621 ggggagcgag gccgtccacg atgagcgccg agctcagcag cgtgttcgac cagaccaggg
     1681 tggcggtgcc gccgtcggcg aggtcgacgc tggcgcgctg cgcgtcatcc gccgagttga
     1741 tggcggcgag ctgatcggcc tgctgctggg cgaagctgtt ctgggcgagc gaggtcgtga
     1801 cgaccccacc gccgacgaac agcgcgatcg cggccgccac cgcggccagc gcagtcaccg
     1861 ggcgggtgaa ccagcgcgcc tgagccttgc ggccggcggg cgactcgaag gccgcggtcg
     1921 ccaccgacgc cgaggaagcg ccagccgccg aggaggccgc aaccggaagc tgcggatgcg
     1981 catccagctg gtccatgatc gacgccttga gtccggccga gggctgcacc ggccgggtcg
     2041 cgagtccgag cagcacagcg gtgtccgtca gctccgtgac ctcgtcgcgg gtggctgggg
     2101 actgccccag gtgagcctcg aattcggcga ggtcagcggc atccagccca ttgagggcgt
     2161 aggagccgga caggttgccg agttccttgt cgctcgtcac gatgccaccc ccatctcgtc
     2221 gcgcaggcgg atcattccgt cacggatacg tgtcttgatc gtgccgatcg gcacgctgag
     2281 aatctcggcg acctcgctgt tgctgtagcc gccgtagtac gacagggtga tcgcctggcg
     2341 ctgcatgtcg gtcagtcgtg ccatggcctt ctccaccctt tcatgttcga ttcgtacttc
     2401 aacggattct gctacgtggt cgtagtcggc gtcgagatcc cggatgccga ccttcacatc
     2461 gcggtcacgg gacgcctgtg aggcgcgcac ccggtccacg gcccgacgat gggtcatggt
     2521 gagaatccag gttgacgctc cccctttgtt cggatcaaaa cgtgaagagg tttgccagat
     2581 ctcgaggaag atctcctggg tgacctcctc cgactgcgcg gggtcgcgca gcaggcgggt
     2641 cacgag
//
LOCUS       DAOSOU010000258         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_39376_length_2610_cov_5.733464, whole genome shotgun sequence.
ACCESSION   DAOSOU010000258 DAOSOU010000000
VERSION     DAOSOU010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39376_length_2610_cov_5.733464"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /gene="miaB"
                     /locus_tag="PLW15_11035"
     CDS             complement(<1..1107)
                     /gene="miaB"
                     /locus_tag="PLW15_11035"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT34968.1"
                     /translation="MTEAVLTAPRTYEVRTYGCQMNVHDSERLSGSLEAAGYVRATEG
                     QADVVVINTCAVRENADNKLYGNLGMLANVKKTKADLQIAVGGCLAQKDKAVILEKAP
                     WVDVVFGTHNMGALPTLLERARHNGEAQLEILESLDVFPSTLPTRRESTHSAWVSISV
                     GCNNTCTFCIVPALRGKEKDRRPGDILAEIQAIVDDGAIEVTLLGQNVNSYGVEFGDR
                     GAFAKLLRAAGTIEGLERIRFTSPHPAAFTDDVIDAMAETPSVMPQLHMPLQSGSDRV
                     LKAMRRSYRSEKFLGILDRVRERIPNAAISTDIIVGFPGETEEDFLDTLAVVERARFA
                     SAFTFQYSIREGTPAATMPDQVPKAIVQERYERLV"
     gene            complement(1143..1772)
                     /locus_tag="PLW15_11040"
     CDS             complement(1143..1772)
                     /locus_tag="PLW15_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOT34969.1"
                     /translation="MSSSGATPSDTAEPLAPVTWLFGEPPIAVAPEELSSPHSTEVAQ
                     QHDPVQRGGFDPINNVSMNALARRGMSIAEMRDYLIGREFEGDEVELELERLMGVALL
                     DDEHLAATLVRTLRERKGLGRSALTAELRRRKLEPAAVESALDDLETGDELERATEIA
                     LRRAPQLRSLDQATAKRRLGAFLMRKGYSGSVVSAAVAAALTPSGPVFR"
     gene            complement(1775..>2610)
                     /gene="recA"
                     /locus_tag="PLW15_11045"
     CDS             complement(1775..>2610)
                     /gene="recA"
                     /locus_tag="PLW15_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT34970.1"
                     /translation="AAKLGVDIDSLLVSQPDTGEQALEIADMLVRSGSIDLIVIDSVA
                     ALVPRAEIEGEMGDSHVGLQARLMSQALRKLTGGLSSTNTTMIFINQLREKIGVFFGS
                     PETTAGGKALKFYASVRLDIRRIETLKDGTDAVGNRTRVKVVKNKMAPPFKQAEFDIL
                     FGVGISREGSLIDFGVEHEIVRKSGAWYTYDGDQLGQGKENSRNFLLENPKIAAEIEQ
                     KIMLKLGIGAEAKAAQTKAAADAIVAEAEQVKAAGAKASGPVLAVATTDEPGLPSAVG
                     E"
BASE COUNT          398 a          856 c          890 g          466 t
ORIGIN      
        1 gacgagtcgc tcataccgct cctgcacgat cgccttgggc acctgatcgg gcatggtggc
       61 cgcgggggtg ccctcgcgga tcgagtactg gaaggtgaat gccgaggcga accgtgcgcg
      121 ttcgacgacc gcgagggtgt cgaggaagtc ctcctcggtc tcgcccggga agccgacgat
      181 gatgtcggtg ctgatcgccg cattcgggat gcgctcgcgc acgcgatcga ggatccccag
      241 gaacttctcg gagcggtacg accggcgcat cgccttcagc acgcggtcgg acccggactg
      301 gagcggcatg tgcaactgcg gcatcactga gggcgtctcc gccatggcgt cgatcacgtc
      361 gtcggtgaag gctgccgggt ggggactcgt gaagcggatg cgctcgagtc cctcgatcgt
      421 gcccgctgcc cgcagcagtt tcgcgaatgc tcctcgatcg ccgaactcca cgccgtagga
      481 gttgacgttc tgacccagca gggtgacctc gatggcaccg tcatcaacga tcgcctgaat
      541 ctccgcgagg atgtccccgg gccgacggtc cttctccttg ccccgcagcg ccggcacgat
      601 gcagaacgtg cacgtgttgt tgcagcccac ggagatcgac acccacgcgc tgtgggtcga
      661 ctcgcggcgg gtcggcaggg tggaggggaa gacgtcgagc gactcgagga tctccagctg
      721 ggcttcgccg ttgtggcgcg cccgttcgag cagtgtcggc agggcgccca tgttgtgagt
      781 gccgaagacg acatcgaccc agggagcctt ctcgaggatg accgccttgt ccttctgggc
      841 gagacatcca ccgacggcga tctgcagatc ggccttggtc ttcttgacgt tggcgagcat
      901 gcccaggttg ccgtagagct tgttgtcggc gttctcacgc acggcacagg tgttgatgac
      961 gacgacgtcg gcctgaccct ctgtggcgcg cacgtagccc gcggcctcca gcgacccgct
     1021 cagccgttcg gagtcgtgca cgttcatctg gcagccgtag gtgcgcacct cgtaggtgcg
     1081 cggggcggtc aggacggctt cggtcatgcc cacaagccta cgacgcggca gaccgtgtcg
     1141 attcagcgga agactggtcc cgacggagtg agagccgccg ctacggccgc ggagacgacc
     1201 gaccccgagt accccttgcg catgaggaag gcacccagcc ggcgcttggc cgtggcctga
     1261 tcgagcgatc gcagctgggg cgcgcgcctg agggcaatct cggtggcgcg ctcgagctcg
     1321 tcaccggtct cgagatcgtc gagcgcggat tccactgctg ctggctccag cttgcgacgc
     1381 cgaagttcgg cggtgagggc cgagcgcccg agccccttcc gctcccgcag cgtgcgcacg
     1441 agcgtcgctg cgaggtgctc gtcgtccagc agcgccacgc ccatgagcct ctcgagttcg
     1501 agctcgacct cgtcgccctc gaactcgcgc ccgatcaggt agtcgcgcat ctcggcgatt
     1561 gacatgcccc tgcgcgccag tgcattcatg ctcacgttgt tgatggggtc gaatcctccg
     1621 cgctgtaccg ggtcgtgctg ctgtgcgacc tcggtggagt gcggggaaga gagctcctcc
     1681 ggcgcgacgg cgatgggggg ttcgccgaac agccacgtca cgggagccaa gggctccgcg
     1741 gtgtcggatg gcgttgcgcc ggaggagctc acgatcactc gcctaccgcg gacggcagac
     1801 cgggctcatc ggtagtcgcg acggcaagca cgggccccga cgccttggcg cccgcagcct
     1861 tcacctgctc agcctccgca acgatcgcgt cggcggccgc cttcgtctga gccgccttgg
     1921 cctcggcgcc gatgccgagc ttgagcatga tcttctgctc gatctcggct gcgatcttcg
     1981 ggttctcgag caggaagttg cgcgagttct ccttgccctg cccgagctgg tcgccgtcgt
     2041 aggtgtacca ggcgcccgac ttgcgcacga tctcgtgctc gacaccgaag tcgatgaggc
     2101 tgccctcgcg cgagatgccc acgccgaaca ggatgtcgaa ctcggcctgc ttgaagggcg
     2161 gcgccatctt gttcttcacg accttgaccc gggtgcggtt tccgacggca tccgtgccgt
     2221 ccttcagggt ctcgatgcgc cggatgtcga ggcgaaccga cgcgtagaac ttcagcgcct
     2281 tgccaccggc ggtcgtctcg gggctgccga agaacacacc gatcttctcg cggagctggt
     2341 tgatgaagat catcgtggtg ttcgtcgagc tgaggccacc ggtgagcttg cgcagcgcct
     2401 gcgacatgag gcgggcctgg aggccaacgt gggagtcacc catctcgccc tcgatctccg
     2461 ctcgcggcac gagggcggcg accgagtcga tgacgatcag atcgatggag ccggaccgca
     2521 cgagcatgtc ggcgatctcg agtgcctgct cgccggtgtc gggctgcgag accagcaggg
     2581 agtcgatgtc gacaccgagc ttggccgcgt
//
LOCUS       DAOSOU010000259         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_39436_length_2607_cov_5.562304, whole genome shotgun sequence.
ACCESSION   DAOSOU010000259 DAOSOU010000000
VERSION     DAOSOU010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39436_length_2607_cov_5.562304"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLW15_11050"
     CDS             complement(<1..263)
                     /locus_tag="PLW15_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT34971.1"
                     /translation="MSPTTITYIGGPTALLEYAGLRFVTDPTFDPPQTYAEPGSTTLV
                     KTAGPGIPRSELGRVDAVLLSHHSHRDNLDYEGLELIATGIPTL"
     gene            complement(280..1131)
                     /locus_tag="PLW15_11055"
     CDS             complement(280..1131)
                     /locus_tag="PLW15_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT34972.1"
                     /translation="MSRTRLELGRREDLGGDCANCFGLCCVALAFSRSSDFPVDKPAG
                     EPCVNLVDDDSCAIHDRLRPSGYRGCTVFDCFGAGQKVSRQTFDGSSWRTDAGVRGEM
                     FAVFPVMRRLHELLWYLDCAIAFTTDEAIGAALAEQFDSVRRLTAGEPADVCEIDTDA
                     LYDQARPLLVRASAAHRGTGARPRPDRRIRPGADLVGVDLRRVDLRAADLRGALLIAA
                     DLRGVDLSRCDLLGVDLRDANVGGADLSSALFLTQPQVSAATGDAATRLPEGFDRPAH
                     WDHNPLT"
     gene            1168..>2607
                     /locus_tag="PLW15_11060"
     CDS             1168..>2607
                     /locus_tag="PLW15_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOT34973.1"
                     /translation="MPHRLAGAASPYLRSHADNPVDWWAWGPEPFAEAARRDVPVLVS
                     IGYSTCHWCHVMARESFSDPHLAEYMNANLVAIKVDREEHAEVDATYLAAAGAFTQNL
                     GWPLNVFVTPQGRAFYAGTYWPPIATQGHPAFREILDAVLQAWRDRRQEVEHNGDLIA
                     EALATRPEVTSAEVDFASVVSDLGAQEDLQYGGFGGAPKFPVAPVLRMLVDRGRGGDA
                     AAAALAARCLDAMAASDLRDADGGFFRYATRRDWTEPHYERMLYDNAQLVASYALAGR
                     AEIAAGVAEFLLTTLQLPGGGFASAQDSESTVAGRRVEGGFYALDLASRASETPPALD
                     EKLLTGWNGLAIEGLAIAGRVLHRADWIEAAQIAADRLLAWHGTSLVRASVDGTVSRA
                     RPTLEDYGMFAGGLIELAIATGEARYAVFARTLVDDAMAAGDPFGVPGGQDPVLAAQG
                     LLVAADDSDGAYPSGLSAIASAAVQLHRLT"
BASE COUNT          413 a          887 c          914 g          393 t
ORIGIN      
        1 agggtcggga tgcccgtggc gatcaactcc aacccctcgt agtcgaggtt gtcgcgatgg
       61 gagtggtggc tgagcagcac ggcatcgacg cgaccgagct ccgacctcgg gatgcccggc
      121 cctgccgtct tgacgagcgt cgtcgatccc ggctcagcgt aggtctgcgg cgggtcgaat
      181 gtcgggtcgg tgacgaagcg caggcccgcg tactcgagca gggcggtcgg cccgccgatg
      241 taggtgatgg tcgtcggcga catacctcga cactagccgt caggtcagcg ggttgtgatc
      301 ccaatgcgct ggccggtcga agccctccgg cagtcgggtc gcggcgtctc cggtggccgc
      361 cgatacctga ggttgggtga ggaacagtgc cgacgacagg tccgccccgc cgacgttcgc
      421 gtccctgaga tcgacaccca ggagatcgca ccgggagagg tcgacgcctc ggagatcggc
      481 tgcgatcaga agcgccccgc gaagatcggc tgccctcaga tcgacccggc gaaggtcgac
      541 gccgaccagg tcggcacccg gccggattcg acggtcgggg cgtggccggg cacccgtgcc
      601 gcggtgggcg gcgctggccc gcacgagcag cggacgcgcc tggtcgtaga gcgcgtcggt
      661 gtcgatttcg cacacatccg ccggttcgcc cgctgtgagc cgacgaactg agtcgaactg
      721 ctcagccaga gcagcaccga tcgcctcgtc ggtggtgaag gcgatcgcgc aatcgaggta
      781 ccagagcagc tcatgaagcc gacgcatcac ggggaacacc gcgaacatct cgccgcgcac
      841 gcccgcatcg gtgcgccacg acgagccgtc gaaggtctgc cgactcacct tctggccggc
      901 accgaagcag tcgaagacgg tgcagccgcg atagccgctc gggcggagcc ggtcatggat
      961 ggcgcaggag tcgtcgtcga cgagattcac acagggttcg ccggcaggct tgtcgacggg
     1021 gaagtctgac gaccgcgaga aggcgagcgc gacacagcac agaccgaaac agttcgcgca
     1081 atctccgccg aggtcttcgc ggcggccgag ctcaaggcgg gttcgtgaca tccttcaaga
     1141 agagtaattg cttggtcaag gagtgccatg ccccatcgac tcgccggtgc cgcgagcccc
     1201 tacctgcgtt cgcacgccga caacccggtc gactggtggg cgtggggacc tgaaccgttc
     1261 gcggaggccg cgcgccgcga cgttcccgta ctcgtgtcga tcggctactc cacctgccac
     1321 tggtgccacg tcatggccag ggagagcttc agcgacccgc acctggccga gtacatgaac
     1381 gccaacctcg tcgcgatcaa ggtcgaccgc gaggagcacg ccgaggtcga cgcaacctat
     1441 ctcgccgccg cgggggcatt cacccagaat ctgggctggc cgctcaacgt gtttgtgact
     1501 ccgcagggcc gagccttcta cgcgggcacc tactggccac cgatcgcgac ccaggggcac
     1561 ccggcttttc gcgagattct cgacgctgtg ctgcaagcct ggcgcgatcg tcgacaggag
     1621 gtcgagcaca acggtgatct cattgccgag gctctcgcaa cccgccccga ggtcacttcg
     1681 gccgaggtcg acttcgcttc ggtcgtgtcc gacctcgggg cccaggagga tctgcagtac
     1741 ggcggcttcg gcggcgctcc caagttcccg gtcgcgccgg tgctgcggat gctcgtcgac
     1801 cgaggcaggg gcggggatgc cgcggccgcc gcactcgcag cgcgctgcct cgacgcgatg
     1861 gccgcctccg atctccggga cgcggacggg ggattcttcc gatacgcgac caggcgcgac
     1921 tggaccgagc cgcactacga gcgcatgctc tacgacaacg cccagcttgt tgcgtcgtac
     1981 gcgctcgcgg gccgcgcgga gatcgccgcg ggggtcgccg agttcctgct cacgacactc
     2041 cagctgcccg gaggcggctt cgcatcggcg caggacagcg agagcacggt cgcggggcgt
     2101 cgggtcgagg gcggcttcta cgcgctcgat ctcgcgtcac gggcatccga gaccccaccc
     2161 gccctcgacg agaagctgct caccgggtgg aacggactcg cgatcgaggg gctcgcgatc
     2221 gcagggcgcg tgctccaccg ggccgactgg atcgaggcag cgcagattgc cgcggaccgc
     2281 cttctggcct ggcatggcac gtctctggtg cgcgcatcgg tcgacggcac cgtgtcgcgt
     2341 gcccggccca cgctcgagga ctacggcatg ttcgcgggtg ggctcatcga gctcgcgatc
     2401 gcgacggggg aggcgcgcta cgccgtgttc gcccgcaccc tcgtggatga cgcgatggcc
     2461 gcgggcgacc cattcggcgt gccgggcgga caggacccgg tgctcgcagc gcaggggctc
     2521 ctggtcgcgg ccgacgacag cgacggtgcc tacccgtccg gactgtcggc gatcgcttcg
     2581 gccgcggtgc aacttcaccg cctgacg
//
LOCUS       DAOSOU010000260         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_40212_length_2570_cov_5.992445, whole genome shotgun sequence.
ACCESSION   DAOSOU010000260 DAOSOU010000000
VERSION     DAOSOU010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40212_length_2570_cov_5.992445"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLW15_11065"
     CDS             <1..850
                     /locus_tag="PLW15_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT34974.1"
                     /translation="TVLDAGLLHDVIGGHDPRDSTSLKDVWPSFADAARAGARAESLK
                     GLRVGVIKELDSPGFQAGVSQRFHEALQLLEANGAEIVEVSAPHFEYAVAAYYVILPA
                     EASSNLAKFDSVRFGLRVNPPGGGTVEDVMAATREAGFGAEVKRRIILGTYALSAGYY
                     DAYYGSAQKVRTLIQRDFAAAFEKADVLVSPSAPTTAFEFGAKLADPLAMYLNDVTTI
                     PANLAGVPGMGLPIGLAPEDGLPVGFQIMAPAREDARLYSLGATLERLLEDAWGHTLL
                     SQAPQL"
     gene            complement(958..1512)
                     /locus_tag="PLW15_11070"
     CDS             complement(958..1512)
                     /locus_tag="PLW15_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HOT34975.1"
                     /translation="MHFAPDTENTLEFAVTLGNTHPSASRSGLDELATPADLTALLDA
                     VTYSGRFDRDVAELREVKETRDHLRRIWTLDADDAVIEVNAMLRDASALPFLTRHDHF
                     GWHLHATDSEAPLAERMRVEVALALVDVIRTGETGRMRVCDAPDCEGLLLDLSRNGSK
                     RFCSVRCGNRMNQLAHRERLGGEG"
     gene            1551..2549
                     /locus_tag="PLW15_11075"
     CDS             1551..2549
                     /locus_tag="PLW15_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT34976.1"
                     /translation="MTHRSTTRGLILGVLASMSFGTSGAFIKPLLEGGWSPAAAVTIR
                     AGIAGLVLLPIAVIALRGRWAALWRARWRVIGMGLIGVAGTQLAYFAAISRIPVSTSL
                     LIEYLAPLILVAIVWIRTRRTPRAVVLIGSVLAVGGLVLIIGPGAIAATDGLGIVFAL
                     IAMLGVVGYYLIAARPSNGLPTVAFAASGLLLGSLTLGVIGGLGLLPLAADFGTVPMG
                     GAEVPWWVPLSFVALVGTAFAYVASIAASEMLGSRLMSFVGLLEVVFAGLFAWLLIGE
                     NISLVQGLGGLLILGGIALVRMEREADAPLEPGSVDEDAGLVSRRGTAATRPAEGA"
BASE COUNT          338 a          803 c          938 g          491 t
ORIGIN      
        1 cacggtgctc gatgccggtc tgctgcacga tgtgatcggc ggtcacgatc cgcgtgacag
       61 taccagcctc aaggatgtct ggccgtcgtt cgccgacgcc gctcgcgccg gtgcccgggc
      121 tgagtcgctc aagggcctcc gtgtcggcgt gatcaaggag ctcgacagcc ccggcttcca
      181 ggccggtgtg tcgcagcgat tccacgaggc actgcagctg ctcgaggcga acggtgccga
      241 gatcgttgag gtgtcggcac cccacttcga gtacgcggtt gccgcgtact acgtgattct
      301 gccggccgag gcatccagca acctggccaa gttcgactcg gtgcgcttcg gcctacgggt
      361 caacccgccc ggtggcggca ccgtcgagga cgtcatggcc gcgacccgag aggccggatt
      421 cggtgccgag gtgaagcgcc gcatcatcct gggcacctac gcgctgtcgg cgggatacta
      481 cgacgcgtac tacggttcgg cacagaaggt gcgcacgctc attcagcgcg acttcgcggc
      541 cgcgttcgag aaggcggacg tgctcgtctc gccctcggcg ccgacgacag cgttcgagtt
      601 cggggcgaag ctcgccgacc cgctggcgat gtacctcaac gacgtgacga cgatccccgc
      661 gaacctcgcg ggtgtgcccg gtatgggcct gccgatcggg ttggcccccg aggacggcct
      721 gccggtcggc ttccagatca tggcgcccgc gcgagaggat gcccggctct actcgctcgg
      781 agcaacactc gagcggctcc tcgaggacgc gtggggtcac accctgctga gccaggcgcc
      841 gcagctgtag tcgggctggt cgagttgccg cgcagctgta gtcaggccgg tagccgcaca
      901 gctgtagtcg ggctggtcga gtagccgcgc agcggcgtat cgaggcccgc ccgccggcta
      961 cccctccccg cccagccttt cccggtgggc gagctggttc atccggttgc cgcagcgcac
     1021 gctgcagaac cgtttggatc cgttgcggct gaggtcgagg agcagaccct cgcaatcggg
     1081 tgcgtcgcac acgcgcatcc ggcccgtctc gccggtgcga atcacgtcga cgagagcgag
     1141 agcgacctcg actcgcatgc gctccgccag gggtgcctcg gagtcggtgg catgcaagtg
     1201 ccagccgaag tggtcgtgcc tggtgaggaa cggcagggcc gaggcatccc ggagcatggc
     1261 gttgacttcg atgacggcgt cgtcggcgtc gagggtccag atgcggcgca ggtgatcgcg
     1321 ggtttctttg acttcgcgta gttctgcgac gtctcggtcg aagcgtccgg agtaggtgac
     1381 ggcgtcgagc agagcggtca gatcggcggg cgtcgccagc tcgtcgagcc cgctgcgcga
     1441 ggcgctggga tgcgtgtttc cgagggtgac cgcgaactcg agagtgttct cggtgtcagg
     1501 ggcaaaatgc aatttgactc cttattctcg acgagtctac tgtcgggact gtgacccatc
     1561 gatccaccac ccgcggcctg attctgggcg tgctcgcatc gatgtcgttc ggtacgtcgg
     1621 gcgcgttcat caagccgctt ctcgagggtg ggtggtcgcc ggcagcggcg gtcacgatcc
     1681 gggcggggat agcgggtctg gtgcttctgc ccatcgccgt catcgccctg cgtggacgct
     1741 gggcggcgct ctggcgcgca cgctggcgcg tgatcgggat ggggttgatc ggggtcgcgg
     1801 gcacgcagct cgcctacttc gcggccatct cccgcatccc ggtgagcacg tccctgctga
     1861 tcgagtatct ggcgcccctc atcctggtcg cgatcgtgtg gattcgcacg cgccgcactc
     1921 ctcgtgccgt ggtgctgatt ggttcggtgc tggccgtcgg tggtctggtg ctcatcatcg
     1981 gcccgggggc gatcgcggca accgacgggc tgggcattgt gttcgcgctg atcgcgatgc
     2041 tcggtgtggt cgggtactac ctgatcgccg cccggcccag taacgggctg ccgacggtcg
     2101 cgttcgccgc gtcgggtctg cttctgggct ctctgacgtt gggggtgatc ggtggtctgg
     2161 gcctccttcc gctggccgcc gatttcggga ccgtgccgat gggcggggcc gaggtgccgt
     2221 ggtgggtgcc gctgagtttt gttgcgctgg tgggcacggc cttcgcgtat gtggcgagta
     2281 tcgcggcatc cgagatgctg ggttcgcgcc tgatgtcgtt cgtcggactg ctcgaggttg
     2341 tcttcgcggg gcttttcgcg tggctgctaa tcggggagaa catctcgctg gtgcagggac
     2401 tgggcgggct gctgatcctg ggtggcatcg cgttggtgcg aatggagcgc gaggccgacg
     2461 cgccgctcga gccgggctcg gtcgatgagg atgcagggtt ggtctcgaga cgcggcactg
     2521 ccgctactcg accggcggaa ggggcgtagg cgctactcga ccgtcggacg
//
LOCUS       DAOSOU010000261         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_40897_length_2537_cov_5.527800, whole genome shotgun sequence.
ACCESSION   DAOSOU010000261 DAOSOU010000000
VERSION     DAOSOU010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40897_length_2537_cov_5.527800"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLW15_11080"
     CDS             complement(<1..342)
                     /locus_tag="PLW15_11080"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HOT34977.1"
                     /translation="MTEARRQELARLVDSRGFIRVTDAAEELGVSDVTIRGDLSELER
                     RGLLTRVHGGAMPVTELRESSLEVGLDRDAAAKAAIGRAAAAMVASGTSIYVDAGSTA
                     MALALALVDRRD"
     gene            341..1483
                     /gene="galT"
                     /locus_tag="PLW15_11085"
     CDS             341..1483
                     /gene="galT"
                     /locus_tag="PLW15_11085"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT34978.1"
                     /translation="MLSNSSAFVTFPFDIVITVLTAGVTKIETTLADGRQLIYYDDPG
                     STLPRERAIDARALDPRPATATMRQDPLTGEWISIAASRQNRAFLPPADQDPLAPATP
                     SNPSEVPSNYDVAVFENRSPSFGPELGHVDLTGVGLGRTAPSFGRCEVVCFSPEHEGS
                     FGSQTITRARTVIEAWADRTEFLSSLPGIAQVFPFENRGEAIGVTLHHPHGQIYSYPY
                     VTPRTQRLIESIGMHGPEFFQDILSFERASERVVLSGEHWTAYVPFAARWPIEVHMLP
                     HRHIPDFAATSETERDELAGLYLRLLRGIDALYDTPTPYIAAWHQAPVSVSRGDIRLM
                     LQVTSPRRAESKLKYLAGSEAAMGAWIGDIPPEQAAAFIREAIARV"
     gene            1480..2298
                     /locus_tag="PLW15_11090"
                     /pseudo
     CDS             1480..2298
                     /locus_tag="PLW15_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
BASE COUNT          398 a          912 c          819 g          408 t
ORIGIN      
        1 gtcccgccgg tcgacgaggg ccagcgcgag agccatcgcg gtactgccgg cgtcgacgta
       61 gatgctggtg ccgctggcca ccatcgccgc ggccgctcgg ccgatggctg ctttggcggc
      121 ggcatcccga tcgaggccga cctcgaggct cgactcccgc agctcggtga ccggcatggc
      181 gcctccgtgc acgcgcgtga gcaggccgcg gcgctccagc tcgctgaggt cgccgcgaat
      241 ggtcacgtca ctgacgccca actcctcggc agcatcggtc acccgaatga acccacgtga
      301 gtcgaccagg cgcgcgagct cctgccgtcg ggcttcggtc atgctttcga attcttccgc
      361 attcgtaacc tttcctttcg atatcgtaat cactgtgctg accgcaggcg tgaccaagat
      421 cgagacgacc cttgccgacg gcaggcagct gatctactac gacgacccgg gctcgacact
      481 cccgcgggag cgcgccattg atgcccgggc cctcgatccc cgccccgcga ctgccacgat
      541 gcgccaggat ccgctgaccg gcgagtggat ctcgatcgcg gcatcccgtc agaaccgggc
      601 cttcctgcct ccagccgatc aggaccccct cgcacctgcg accccgagca acccctcgga
      661 ggtgcccagc aactacgacg tcgccgtgtt cgagaaccgc tccccgtcgt tcggacccga
      721 actcggccac gtcgatctca ccggcgtcgg cctcgggcgc accgcgccat cattcgggcg
      781 ctgcgaggtc gtctgcttca gcccggagca cgagggatcg ttcggctcgc agacaatcac
      841 ccgcgcgcgc accgtgatcg aggcctgggc cgaccgcacc gagttcctca gctcgctgcc
      901 gggcattgcg caggtgttcc ccttcgagaa tcgcggcgag gcgatcggcg tgaccctgca
      961 ccacccgcac ggccagatct acagctaccc ctacgtgacg ccgcgcaccc agcgcctcat
     1021 cgagtcgatc ggcatgcacg gtcccgagtt cttccaggac atcctgtcgt tcgagcgggc
     1081 atcggagcgc gtcgtgctca gtggcgagca ctggaccgcc tacgtgccct tcgccgcgcg
     1141 ctggccgatc gaggtgcaca tgctgccgca ccgtcacatc cccgacttcg cggcgacgag
     1201 cgagaccgag cgcgacgaac tcgccgggct ctacctgcgg ctgctgcgcg gcatcgacgc
     1261 gctctacgac accccgaccc cctacatcgc agcctggcac caggcgccgg tgagtgtctc
     1321 gcgcggcgac atccggctga tgctgcaggt gaccagcccg cgtcgggcag aatcgaagct
     1381 caagtacctc gcaggctcgg aggccgcgat gggcgcctgg atcggcgaca tcccgccgga
     1441 gcaggcagcg gcattcatca gagaggcaat cgcgcgagta tgagcgacat cagacaggac
     1501 gtggtcgcgg gcttcgagga ggcctacggc tcggcgccct cgggcatctg gtcggctccc
     1561 gggcgcgtca acctcatcgg cgaacacacc gactacaacc tcggcttcgt gctgccgttc
     1621 gccatcaacc gccgcacgat cgtggccctc ggcccgcgtg acgacggccg gatcggcgtc
     1681 gcgagcgcct acgccgacga ggtcgccgag atcgcgatcg ccgacctggc ccccgaccga
     1741 ctgcgcggct gggccgccta cccgctgggg gtcgcgtggg ctctgggcca gcacggtgct
     1801 gacttggccg ccgttcccgg cgtgaacctc ttcatcgagt cgacagtgcc ggtgggtgcg
     1861 ggtctgtcgt cgtcggcggc gatcgagagc gccgtcgcgc tcgcactcaa cgacgtgtgg
     1921 cagctcggct tcgaccggcc gaccctcgcc aaggtcggtc agcttgccga gaacaaggcc
     1981 gtgggcgcgc cgaccggcat catggaccag tcagcgtcgc ttctcgggcg cgaagaccac
     2041 gcggtgttcc tcgactgccg cagtctcgag tcccatctcg tccaactcgg gttcgccgag
     2101 gcccgcctcg aggtgctggt catcgatacc ggggtcaagc actcgcatgc caccggcggg
     2161 tatgcgtcgc ggcgggcatc ctgcgaggcg ggcgcgaagg cgctcgaggt cgcctcgctg
     2221 cgcgatctga ccgtcgacga cctgccccgg gcccgcgccc tgctcgacga cgagacgttc
     2281 cgccgggtgc ggcacgtgga aatgaaacat atctgtcggg tcgccgtccg ttgagaggcg
     2341 gccacgagaa ctggaagtgc ttggaatgct gcaagtcgag ttatgggcgc gtgcgtgcaa
     2401 gaggagtcga tgaaactcac cgacatcggc gacccgaatt actttcacaa ggtcgttgac
     2461 tgccaatggg cctgtccggc gcatacgccg gttcccgaat atatccgcct gatcgcgcag
     2521 gggcgttatt ccgacgc
//
LOCUS       DAOSOU010000262         2505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_41601_length_2505_cov_3.014286, whole genome shotgun sequence.
ACCESSION   DAOSOU010000262 DAOSOU010000000
VERSION     DAOSOU010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41601_length_2505_cov_3.014286"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLW15_11095"
     CDS             complement(<1..261)
                     /locus_tag="PLW15_11095"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT34979.1"
                     /translation="MSDLRDDPTSPPVLSSERVFDGRVWDVRRETFELGDATILRDFV
                     DHPGAVAVLAMDERDRVLLIKQYRHPVRSRDWELPAGLLDITG"
     gene            complement(254..1951)
                     /locus_tag="PLW15_11100"
     CDS             complement(254..1951)
                     /locus_tag="PLW15_11100"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT34980.1"
                     /translation="MVDKSSAAVTKHIFVTGGVVSSLGKGLTAASLGNLLTARGLRVV
                     MQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDINLNQAANVTTGQI
                     YSTVIARERRGEYLGDTVQVIPHITDEIKRRMRLQAQPGPAGEPAPDVIITEIGGTVG
                     DIESQPFIESARQVRHELGRKNVFFVHVSLVPYMGASGEQKTKPTQHSVAALRSIGIQ
                     PDALVLRSDRPVSDSNKRKIALMCDVDEAAVVNAVDVPSIYDIPTMLHDQGLDAYIIE
                     QLGLVAGEVNWDGWSDLLKAVHDPKDEVTIGLVGKYIDLPDAYLSVTEALKAGGFAHQ
                     TKVVIKWVPSDLCEAPEGAAKMLGDLDGMLVPGGFGVRGIEGKLGALKFARENGLPTL
                     GLCLGLQCMVIEYARDVVGLAGASSSEFDPDTQYPVIATMAEQVDIIDHGDMGGTMRL
                     GLYDAALEPGSIVEELYGAPTVAERHRHRYEVNNAYRDQIAEAGLSFSGISPDRTLVE
                     FVELPRDVHPFYVGTQAHPELRSRPNHAHPLFRGLVDAALSRQQASRLFEVPDPPDE"
     gene            complement(2026..>2505)
                     /locus_tag="PLW15_11105"
     CDS             complement(2026..>2505)
                     /locus_tag="PLW15_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT34981.1"
                     /translation="EDRPDYSLTGKDQVSILLQPHPGAEPRPISKGASGGELSRVMLA
                     IEVVLAGTDPVPTFVFDEIDAGVGGASATEVGRRLAHLAESAQVIVVTHLAQVAAFST
                     NHLVVEKGTDGSITSSNVRLVHAEERTAEMARLLSGNPDSETALAHARELLEAAHAR"
BASE COUNT          394 a          824 c          867 g          420 t
ORIGIN      
        1 ccccgtgatg tcgagcagtc cggccggcag ctcccagtcg cgcgagcgca ccggatgccg
       61 gtactgcttg atcagcagca cgcgatcgcg ctcatccatg gcgagcaccg ccacggcacc
      121 cgggtgatcg acgaagtccc gcaggatggt ggcgtcaccg agctcgaagg tctcgcgccg
      181 cacatcccag acccgcccgt cgaagacgcg ctcgctggag agcaccgggg gcgaggtggg
      241 gtcgtcgcgc aggtcactca tccggcggat ccggaacctc gaacaaacga ctggcctgct
      301 ggcggctcag cgccgcatcg accaggccgc ggaacagcgg atgcgcgtgg ttgggccgcg
      361 agcgcagctc cgggtgcgcc tgcgtaccca cgtagaacgg gtgcacgtcg cgcggcagct
      421 cgacgaactc gacgagcgtg cggtcgggcg agatacccga gaagctcagg cctgcctcgg
      481 cgatctggtc gcggtaggcg ttgttcacct cgtagcggtg acggtggcgc tcggccacgg
      541 tcggagcgcc gtacagctcc tcgacgatcg acccgggctc gagcgccgcg tcgtagaggc
      601 ccaggcgcat ggtgccaccc atgtcgccgt ggtcgatgat gtcgacctgc tcggccatgg
      661 tggcgatcac ggggtactgc gtgtcggggt cgaactccga cgaggatgcc ccggcgagcc
      721 ccaccacgtc gcgggcgtac tcgatcacca tgcactgcag gccgaggcac agtccgagcg
      781 tcggcaggcc gttctcgcgc gcgaacttca gcgcgcccag cttgccctcg attccgcgca
      841 cgccgaaacc gccgggaacg agcatgccgt ccaggtcgcc cagcatcttg gcggcgccct
      901 cgggtgcctc gcacaggtcg gagggcaccc acttgatgac gaccttggtc tggtgcgcga
      961 aaccgccggc cttgagcgcc tcggtgacgg acaggtacgc gtcgggcagg tcgatgtact
     1021 tgccgacgag tccgatggtc acctcgtcct tggggtcgtg cacggccttg agaaggtcgc
     1081 tccagccgtc ccagttgacc tcgccggcca cgaggccgag ctgctcgatg atgtaggcgt
     1141 cgaggccctg atcgtggagc atcgtgggga tgtcgtaaat gctgggaacg tccaccgcat
     1201 tgaccacggc ggcctcgtcg acgtcgcaca tgagcgcgat cttgcgcttg ttgctgtcgg
     1261 agaccgggcg gtcgctgcgc agcacgagcg cgtcgggctg gatgccgatg gaccgcagcg
     1321 ctgcgaccga atgctgggtg ggcttggtct tctgctcccc cgaggcgccc atgtagggaa
     1381 ccagggagac gtgcacgaag aacacgttct tgcgaccgag ctcgtggcgc acctggcgcg
     1441 ccgactcgat gaagggctgg ctctcgatgt caccgaccgt tccaccgatc tccgtgatga
     1501 tcacgtcggg ggcgggctcg ccggcgggtc cgggctgcgc ctgcaggcgc atccgtcgct
     1561 tgatctcgtc ggtgatgtgc gggatgacct gcaccgtgtc accgaggtac tcgcccctgc
     1621 gctcccgggc gatcacggtc gagtagatct ggccggtggt gacgttggcg gcctggttga
     1681 ggttgatgtc gaggaatcgc tcgtagtggc cgatgtcgag gtcggtctcg gcgccgtcat
     1741 cggtgacgaa cacctcgccg tgctggaacg ggttcatcgt tccgggatcg acgttgaggt
     1801 acggatccag cttctgcatc acgacgcgga gtccgcgagc ggtgagcaga ttgcccagac
     1861 tggcggcggt gaggccttta ccgagcgagg agacgacgcc cccggtcacg aagatgtgct
     1921 tggtaacggc cgctgaagat ttatccacca cggggtttca tcgtatccga ctaattgacc
     1981 cgcactcccc gacgcgctgt gcccagggcg aacgaaacac gcgactcagc gcgcgtgtgc
     2041 cgcctcgagc agttcgcgcg cgtgcgcgag ggccgtctcc gagtcgggat tgccgctcaa
     2101 cagccgcgcc atctcggccg tgcgctcctc ggcgtgcacc aggcgcacgt tgctggaggt
     2161 gatggatccg tcggtgccct tctcgaccac gagatggttc gtggagaagg cagcgacctg
     2221 cgcgaggtgg gtgacgacga tcacctgggc ggattcggca aggtgcgcaa gccgtcggcc
     2281 tacttcggtc gcgctggcac cgccgacacc cgcgtcgatc tcgtcgaaca cgaacgtcgg
     2341 caccgggtcg gtgcccgcga gcacgacctc gatagccagc atcacacgcg acaactcgcc
     2401 gcccgaggcg cccttgctga tcggtctggg ttcggcaccc ggatgcggct gcagcagaat
     2461 cgacacctgg tctttgcccg tcagcgagta gtccggccga tcctc
//
LOCUS       DAOSOU010000263         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_42215_length_2478_cov_6.808089, whole genome shotgun sequence.
ACCESSION   DAOSOU010000263 DAOSOU010000000
VERSION     DAOSOU010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42215_length_2478_cov_6.808089"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..470)
                     /locus_tag="PLW15_11110"
     CDS             complement(60..470)
                     /locus_tag="PLW15_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34982.1"
                     /translation="MSTADDAKVNDPRNPLLVLLAVLLFAECALLVAATVYLVVELLI
                     ATPASLASALFLTVLTGIAAAWLGVIAVNTLRASPWIRGAAITWQVLQIAVAIGCFQG
                     LFAQPTIGWALLVPALAVLVLVFTPRVVQATKRT"
     gene            656..904
                     /locus_tag="PLW15_11115"
     CDS             656..904
                     /locus_tag="PLW15_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT34983.1"
                     /translation="MDWRDKAACLTADPELFFPVGNTGPAVDQIDKAKAVCGRCSVTE
                     MCLQYALDTSQDSGVWGGLSEDERRALKRRAARARRAS"
     gene            complement(940..2454)
                     /locus_tag="PLW15_11120"
     CDS             complement(940..2454)
                     /locus_tag="PLW15_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HOT34984.1"
                     /translation="MSTLSDLVQAQGRSSEADIEWLHLLVGDWQLLADLAFADIVLWV
                     PSAEGGFVAVSHARPSSSATLFYRDFVGQAIKPEWKAQVTEAFETSRIVDTATPDWYE
                     ETPTRVRAVPVLRRLSPSGSATTKTPIAVITRHTNLSEARTPSRQELTFNECANDLFA
                     MIASGDFPDLGAPSGPRRGAPRASDGLIRLDVDGMVTFASPNALSAFNRMGFAGELEG
                     ESLAEVTTSLLHGRLTVDESLPLVVTGRAPWRTDIESRGVTVSLRTIPIRNRGERVGA
                     IVLCRDATEIRHQERELLTKDATIREIHHRVKNNLQTVASLLRIQARRTHSDVARDAL
                     GQAMRRVAAIAVVHDTLSEGLNQNVDFDVVFDRVLKLIAEVASSHNTTVHPTSTGSFG
                     HLPSEYATPLALALTELVTNAVEHGLAGHADGKVDIIAKRTDDLLSVKVRDNGVGLPE
                     GKVGSGLGTQIVRTLIQGELGGTIDWHTLMGSGTEVTIDVPMTWMKKSSAQAAG"
BASE COUNT          394 a          842 c          832 g          410 t
ORIGIN      
        1 ccgttcacgc ccgaggatcc gctgatcgtc gccaagcaga acccgagcga cccggtcgcc
       61 taggtgcgct tcgtcgcctg cacgacgcgc ggagtgaaca ccagcacgag cacggcgagg
      121 gcgggaacca gcagcgccca gccgatcgtc ggctgcgcga agaggccctg gaagcatccg
      181 atcgccacgg cgatctgcag cacctgccag gtgatggcgg ccccgcggat ccaggggctg
      241 gcgcgcaggg tgttcaccgc gatgaccccg agccatgctg cggcaatgcc ggtgagcacc
      301 gtcaggaaga gcgcgctggc cagcgatgcg ggcgtcgcaa tgagcagttc gacaacgagg
      361 tagacggtcg cggcgaccag cagtgcacac tcggcgaaca gcagaacggc gagcagaacg
      421 agcagcggat tccggggatc gttcaccttc gcgtcatccg ctgtactcac agtgtcgtcc
      481 catacaaaac cattgatttg catctaccgg tgtgagagtc tatatgaggt tgatttcacg
      541 ctcccagtgt cgcgggcgga tcccccagga attctcaccc gatacaccag atggctgttt
      601 tcgtcagcgt ctgccccgcg gtctagtcca ccgcgtgcac cgaaggagca acaccatgga
      661 ttggcgcgac aaggctgcct gcctcaccgc agaccccgag ctcttcttcc cggtcggtaa
      721 caccggcccg gctgtcgacc agatcgacaa ggcgaaggcc gtgtgcggtc gctgctcggt
      781 gaccgagatg tgcctccagt acgcgctcga cacctcgcag gactccggcg tctggggtgg
      841 cctcagcgaa gacgagcgtc gcgccctcaa gcgtcgcgct gcccgcgcgc gccgcgcttc
      901 ctagtcgctc cgggtttcga tacgcggctt cgccgccact caacccgccg cctgcgctga
      961 cgacttcttc atccacgtca tcggcacgtc gatggtgacc tcggtgccgc tgcccatgag
     1021 cgtgtgccag tcgatcgttc cgcccaactc gccctggatg agggtgcgca cgatctgagt
     1081 tccgagccct gagccgacct tgccctcggg caacccgacc ccgttgtcgc gcaccttcac
     1141 gctcagcagg tcgtcggtgc gcttggcgat gatgtcgact ttgccatccg cgtgacccgc
     1201 gagaccgtgc tccaccgcgt tggtcaccaa ctcggtcagg gcgagcgcga gcggcgtcgc
     1261 gtactcgctc ggcaggtggc cgaagctgcc cgtcgaggtc gggtgcaccg tcgtgttgtg
     1321 gctcgaggcg acctccgcga tgagcttcag tacccggtcg aacaccacgt cgaagtcgac
     1381 gttctggttg agtccctcgc tgagggtgtc gtgcaccacg gcgatggcgg cgacgcggcg
     1441 catggcctgg ccgagcgcat cccgggcgac gtcgctgtgg gtgcggcggg cctggatcct
     1501 gagcagggat gccacggtct gcaggttgtt cttgacccgg tggtggatct cgcggatcgt
     1561 ggcatccttc gtcaggagtt cgcgctcctg gtggcggatc tccgtggcat cccggcacag
     1621 cactatcgcg ccgacgcgct ccccgcgatt gcggatcggg atggtgcgca gcgacaccgt
     1681 caccccgcgc gactcgatgt cggtgcgcca cggtgcccgg ccggtgacca ccagcggcag
     1741 cgactcgtcg acggtgagcc gcccgtgcag caggctcgtg gtgacctcgg cgagcgactc
     1801 gccctcgagc tcgcctgcga aacccatgcg gttgaacgcc gacagcgcgt tggggctcgc
     1861 gaacgtcacc atgccgtcga cgtcgagcct gatcagacca tcggatgccc ggggtgcccc
     1921 acgacgcgga ccactcggcg cacccagatc gggaaagtcc ccggaggcga tcatcgcgaa
     1981 caggtcgttg gcgcactcgt tgaaggtgag ctcctggcga ctcggggtgc gcgcctcgct
     2041 caggttggtg tgccgggtga tcaccgcgat cggagtcttc gtggtcgcgc tgccgctcgg
     2101 gctcagcctg cgcagcaccg gcacggcgcg cacgcgcgtg ggcgtctcct cgtaccagtc
     2161 gggcgtggcg gtgtcgacga ttcgggatgt ctcgaaggcc tcggtgacct gcgccttcca
     2221 ctcgggcttg atggcctggc cgacgaagtc gcggtagaac agggtcgccg agctcgacgg
     2281 gcgcgcgtgc gagacggcga cgaaaccgcc ctcggcgctc ggcacccaca gcacgatgtc
     2341 ggcgaaggcg aggtcggcga gcagctgcca gtcgccgacg agcaggtgca gccactcgat
     2401 gtcggcctcg ctcgaacgcc cctgggcctg aacgagatca ctgagggtcg acacgcgttc
     2461 aaggctagcg ccctagcg
//
LOCUS       DAOSOU010000264         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_42263_length_2476_cov_5.451053, whole genome shotgun sequence.
ACCESSION   DAOSOU010000264 DAOSOU010000000
VERSION     DAOSOU010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42263_length_2476_cov_5.451053"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="PLW15_11125"
     CDS             <1..1879
                     /locus_tag="PLW15_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT34985.1"
                     /translation="DTVMSGALVSSGTALVLVTAVGADSYANRLTSEIRRHSLAHSEL
                     RAATNRILVYISWILGPIVLVVVGSRMLTYGNGFSLAALGDGRWRTALLDVVASVVGM
                     IPEGLVLLISLTFGVAAIRLARQRVLIQELAAVEILARVDVLCLDKTGTLTSGDVAYR
                     TFELFSDDPRLEAALGLFGHDDAANATARALTTHFPPTAATAIRRIPFASSRAFSALE
                     LALDGEESRWLLGAPERLLSGEQRVRAAVHAGDGTRTLAVVRVEGHLPETDDLVLDEL
                     TLHPVALLLFGETVRPDAAATLGYFAEQGVRCVIISGDNPTTVAALADGLGVGSSSVD
                     ARTLTDDAALRSALSEHSIFGRVTPDQKRAMVRVLQEDGHTVAMTGDGVNDAMAIKDA
                     DLGIAMGSGTSASKAVARVVLLDNEFGRLPNVLLYGRQVIANVERVANLFLAKTSYGI
                     LFALVFAVLLWEFPFLPRQLTLVSTLTIGIPAFFLALAPNRRVYHPGILGRLLRYAVP
                     TGTIAAVIAFATNAIVRQWVPQDEARSITTIALWIVAFWILCVLSRPLDRWRFLLLTA
                     MAVLFVLANTVPFARDFFAMHQQFDWPLAVGVALGCLGAAAIELTYRVARSRGLIFDR
                     E"
     gene            1972..>2476
                     /locus_tag="PLW15_11130"
     CDS             1972..>2476
                     /locus_tag="PLW15_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOT34986.1"
                     /translation="MSAVNSFGSKDTLTVGSTDYEVFRVDTVPGHEKLPFSLKVLLEN
                     LLRTEDGANVTKAQIEALGSWVPTAEPDTEIQFTPARVVMQDFTGVPCIVDLATMREA
                     MSALGGDPTKINPLAPAELVIDHSVIADLFGTEDALERNVEIEYERNGERYQFLRWGQ
                     TAFDDFKV"
BASE COUNT          384 a          846 c          791 g          455 t
ORIGIN      
        1 cgacacggtc atgtcgggcg ccctagtgtc cagcggaacc gcgctggtgc tggtcacggc
       61 ggtgggtgct gactcgtacg ccaaccggct gacctccgag attcggcgac actcgctcgc
      121 gcattcggag ctgcgcgcgg cgaccaatcg catcctcgtc tacatttcgt ggattctcgg
      181 tccgatcgtg ctcgtggtcg tcggcagccg gatgctcacc tacggcaacg gattcagtct
      241 cgccgcgctc ggcgacggtc gctggcgtac ggcactgctc gatgtcgttg cgagcgtggt
      301 gggaatgatc cccgagggtc tcgttctgct catcagcctg accttcgggg tcgcggccat
      361 ccgtcttgcc cgtcagcgtg tgctgattca ggaactggcg gcggtagaga ttctcgcccg
      421 cgtcgatgtg ctgtgcctcg acaagaccgg aacgttgacc agcggggatg tcgcgtaccg
      481 caccttcgag ctcttctcgg acgatccgcg cctcgaggcc gccctcggac tgttcggcca
      541 cgacgacgcc gccaacgcga cggcgcgagc tctgacgacc cacttccctc ccacggcggc
      601 cacagccatt cgacgcatac cgttcgcaag ctcgcgcgcc ttcagcgcac tcgagctcgc
      661 gctcgacggc gaggaatcac gctggctact gggggcgccc gaacgactgc tgtcgggtga
      721 gcaacgggtc cgcgctgccg tccacgccgg cgatggaaca cgcacgctgg cggtcgtgcg
      781 ggtcgagggc cacctccccg agaccgatga cctcgtgctc gacgagctca cgctgcaccc
      841 cgtcgcactg ctgctcttcg gcgagaccgt tcgaccggac gccgccgcca ccctcggcta
      901 cttcgccgag cagggcgtgc gctgcgtgat catctccggc gacaacccga cgaccgtcgc
      961 cgccctcgcc gacggcctgg gggtcgggtc gagctcggtc gatgcgcgca ccctgacgga
     1021 tgacgccgca ctgcggtcgg cactgtccga gcacagcatc ttcggccgtg tcacccccga
     1081 ccagaaacgg gccatggtgc gcgtgctgca ggaggacggc cacaccgtcg cgatgaccgg
     1141 cgacggtgtg aacgacgcaa tggcgatcaa ggacgccgat ctcggcatcg cgatgggctc
     1201 gggcacgtcg gcatcgaagg cggtcgccag ggtggtactc ctcgacaacg agttcggtcg
     1261 gctccccaat gtgctgctgt acgggcggca ggtgatcgcg aacgtggagc gcgtcgcgaa
     1321 tctgttcctc gcgaagacga gctacggcat ccttttcgcc ctagtgttcg cggttctgct
     1381 ctgggagttc cccttcctcc ctcgccagtt gaccctggtg tccacgctca ccatcgggat
     1441 cccggcgttc ttcctcgcgc tggcgccgaa ccggcgcgtc taccatccgg gcatcttggg
     1501 aaggctgctg cgctacgccg tgcccaccgg aacgattgcc gccgttatcg ccttcgcgac
     1561 gaacgcgatc gtgcgtcagt gggtcccaca ggatgaggct cgctcgatca cgacaatcgc
     1621 gctgtggatc gtcgccttct ggattctctg cgtgctgtcg aggcctctcg atcggtggcg
     1681 attcctcctg ctcacggcca tggctgtact gttcgttctc gccaacacgg ttcccttcgc
     1741 gcgcgacttc ttcgccatgc accagcagtt cgactggccc ctcgcggtcg gcgtcgcgct
     1801 cggctgtctg ggagccgcgg caatcgaatt gacctaccgc gtggcgcgct ctcgcggtct
     1861 gatcttcgac cgggagtgat gctagattta tcttgacgtc aagatagttt cggcggaacg
     1921 gtgcgggcca tccgtgcaag gattgtcgca aggtcagcaa gggagacgac agtgtcagca
     1981 gtgaacagct tcgggtcgaa ggacaccctc acggtcgggt cgaccgacta cgaggtgttc
     2041 cgcgtcgata ccgttccggg acacgagaag ttgcccttca gcctcaaggt gctgctggag
     2101 aatctgctgc gcaccgagga tggcgccaac gtcaccaagg cgcagatcga ggctctcggc
     2161 tcgtgggttc cgaccgccga acccgacacc gagatccagt tcacgcccgc gcgcgtcgtg
     2221 atgcaggact tcaccggcgt gccgtgcatc gtggacctcg cgaccatgcg cgaggccatg
     2281 agcgcgctcg gtggcgaccc cacgaagatc aacccgctcg cgcccgccga gctggtcatc
     2341 gaccactcgg tgatcgccga cctgttcggc accgaggacg ccctcgagcg caacgtcgag
     2401 atcgagtacg agcgcaacgg tgagcgctac cagttcctgc gctggggcca gaccgccttc
     2461 gacgacttca aggtcg
//
LOCUS       DAOSOU010000265         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_42893_length_2450_cov_6.963257, whole genome shotgun sequence.
ACCESSION   DAOSOU010000265 DAOSOU010000000
VERSION     DAOSOU010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42893_length_2450_cov_6.963257"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLW15_11135"
     CDS             <1..348
                     /locus_tag="PLW15_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT34987.1"
                     /translation="RHVVTENQRVLDTVDRLRTDGPLAIGELLDASHVSMRDDFEISV
                     PELDLAVETAQANGAIGARMTGGGFGGAAIALVPSDGISRIQVAIDGAFAEHGFAQPT
                     MFTVTASNGAARD"
     gene            391..1593
                     /locus_tag="PLW15_11140"
     CDS             391..1593
                     /locus_tag="PLW15_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT34988.1"
                     /translation="MIGRIEHRLGVIHLELFARLSSHSWRRLPHPPFEGTVARGAGHD
                     PDRILLIGRAGAASWGVVSHELGLGGHLARATASLTGRGVDVEISGDPALSLPVIQQR
                     LTQAAISRYDAIVLTIGLREAMELMPVTVWTRQLTSLLDHVSAGREVSPAVVVVGAEE
                     RLPVPFTPGMARLGTSRARALNEASRTVIGDRTRVRFVDSGMVPEMENSTLLDANKLD
                     VYERSARVIAPTLATLLETSPARLRHPVDEEARARALEYLRSRDRADDARLAQLLQTV
                     KEVLHLRSADLFFVDRDEVHMLGTTTEAVSSRPRSETFSTDALEYRGGIVIPDLTDHP
                     RLRSLPEVTGPPHLRFYAAHPVESPDGHRVAVLSVVDTVPRDFSAAEAGALRQLALQV
                     GTILFEDY"
     gene            complement(1685..>2450)
                     /locus_tag="PLW15_11145"
     CDS             complement(1685..>2450)
                     /locus_tag="PLW15_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOT34989.1"
                     /translation="EVYESMLAADTPLIVTDYATAELVKVAANAFLATKISFINAMAE
                     IAEATGADVTQLADAIGHDARIGRRFLNAGVGFGGGCLPKDIRAFTARAEELGRGESI
                     AFLKEVDAINLRRRQRVVDLVVDALGGEPNDKRVAVLGLAFKPDSDDVRDSPAVDVAI
                     QLRGLGAKVSATDPQAIPTARRLHPQLKYKSDVSEALAGAEVVVLVTEWREYRELDPV
                     AVGELVASRVIIDGRNCLDAAAWRAAGWTYIGMGRP"
BASE COUNT          369 a          842 c          857 g          382 t
ORIGIN      
        1 cggcacgtgg tcaccgagaa ccagcgggtg ctcgacaccg tcgaccgctt gcgcaccgac
       61 ggccccctgg cgatcggcga gctgctggat gcctcgcacg tctccatgcg cgacgacttc
      121 gagatcagcg tgcctgaact cgacctcgcc gtcgaaaccg cgcaggccaa cggagccatc
      181 ggagcacgga tgaccggcgg cggtttcggt ggggcggcga tcgcgctcgt tccctcggac
      241 gggatctcgc gcatccaggt cgccatcgac ggcgcgttcg ccgagcacgg cttcgcacag
      301 ccgacgatgt tcacggtgac cgcctcgaat ggggcggcgc gggactgaca cctcccggag
      361 tatcctgacg ctcagtcgcg aggagcgttc atgatcggca ggatcgagca ccggctcggg
      421 gtgatccacc tcgagctgtt cgcgcggctg tcgagccact cgtggcggcg actgccgcat
      481 ccgccgttcg agggaaccgt ggcccgcggg gctggccacg accccgatcg gattctgctc
      541 atcgggcgcg cgggcgccgc gagctggggc gtggtgagcc acgaactcgg gctgggcggg
      601 catctcgcgc gagcgaccgc gtccctcacc ggccgtggtg tggatgtcga gatctcgggt
      661 gatccggccc tgtcgctgcc cgtcatccaa caacgcctga ctcaggccgc aatctcgcgc
      721 tacgacgcga tcgtgttgac cattggcctc cgagaggcga tggagttgat gccggtgacg
      781 gtgtggacga gacagctgac gtctctgctc gatcacgtct ccgctggccg tgaggtgagc
      841 ccggccgtcg tcgtggtggg ggcggaagaa cgactgcccg tgccgttcac ccctggcatg
      901 gcacgactgg gaacgtcgcg ggcccgagct ctcaacgagg cgtctcggac ggtgatcggg
      961 gaccgcacac gggtgcgttt tgtcgactcc gggatggtgc ccgagatgga gaattcgacc
     1021 ctgctggatg ccaacaagct cgacgtgtac gaacgaagtg cgcgagtgat cgccccgacg
     1081 ctcgcgacgc tgctcgagac cagccccgcc cgactgcgtc atccggttga cgaggaggct
     1141 cgcgctcgcg ctctggaata tctgaggtcg cgcgatcgcg ccgatgacgc gcggttggcc
     1201 caactcctgc agaccgtgaa ggaggtgctc catctgcgca gcgcggacct gttcttcgtc
     1261 gaccgcgacg aggtgcacat gctcggcacg acgaccgagg cagtgtcgag ccgtccgcga
     1321 agtgagacct tcagcaccga cgcactcgag taccggggcg gcatcgtgat ccctgacctg
     1381 accgaccacc cgcggctcag aagcctgccc gaggtgaccg ggccgcccca cctgcgcttc
     1441 tacgcggcac acccggtcga gtcgccggac ggccaccggg tcgccgttct ctcggtggtc
     1501 gacacggttc cgcgcgactt ctcagccgcc gaggcgggag cgctgcgtca gctcgccctc
     1561 caggtcggca cgatcctctt cgaggactac tgagcactcc aaccacggct ggcccagtcg
     1621 cggggtgcgg tcacggccgg ctcaatggcg gggtggccac ggccggccca gcgccgatgt
     1681 gcggtcacgg ccggcccatg ccgatgtagg tccacccggc cgcgcgccaa gctgcggcgt
     1741 cgaggcagtt gcgaccgtcg atgatgaccc gggatgccac gagctccccc accgcgacgg
     1801 ggtcgagctc gcggtactcg cgccactcgg tgaccagcac cacgacttcg gccccggcga
     1861 gcgcctcgga cacatcggac ttgtacttca gctgcgggtg gaggcgccgc gcagtgggga
     1921 tcgcctgcgg gtcggtggcg gagaccttcg caccgagccc gcgcagctgg atcgcgacat
     1981 ccactgccgg ggaatcgcgc acgtcgtcgg agtcgggctt gaaggccagc ccgagcaccg
     2041 cgacccgctt gtcgttcggc tcgccaccga gggcatcgac cacgaggtcg acgactcgct
     2101 gccggcgccg gaggttgatc gcgtcgacct ccttgaggaa ggcgatcgac tcgccgcgac
     2161 cgagctcctc ggcgcgggcg gtgaaggccc ggatgtcctt gggcaggcag ccgccgccga
     2221 acccgacacc ggcgttgagg aagcggcgcc cgatgcgcgc gtcgtgcccg atcgcgtcgg
     2281 ccagctgggt gacgtcggcg ccggtcgcct cggcgatctc cgccatcgca ttgatgaacg
     2341 agatcttggt tgccaggaag gcgttggccg cgaccttgac cagttcggcc gtggcgtagt
     2401 cggtcacgat gaggggcgtg tcggcggcca gcatcgactc gtagacctcc
//
LOCUS       DAOSOU010000266         2442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_43066_length_2442_cov_5.757855, whole genome shotgun sequence.
ACCESSION   DAOSOU010000266 DAOSOU010000000
VERSION     DAOSOU010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43066_length_2442_cov_5.757855"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLW15_11150"
     CDS             complement(<1..420)
                     /locus_tag="PLW15_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide succinyltransferase"
                     /protein_id="HOT34990.1"
                     /translation="MSESVNLPALGESVTEGTVTRWLKKVGDRVEVDEPLLEVSTDKV
                     DTEIPSPVAGVIEAVLVAEDETVEVGTTLVTIGDGSGATEAPAAPAPVAEAPAPAPEP
                     VAEASAPVVEAPAPAPVVEAPAPAPVVEAPAPAPVVET"
     gene            complement(481..1854)
                     /gene="lpdA"
                     /locus_tag="PLW15_11155"
     CDS             complement(481..1854)
                     /gene="lpdA"
                     /locus_tag="PLW15_11155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT34991.1"
                     /translation="MAEHNFDLVVLGGGSGGYAAAVRASQLGMSVGLIEKNKVGGTCL
                     HVGCIPTKALLHSAEVADVTRESAKYGVSSTFNGVDIAAVTGYRQEVVASKYKGLQGL
                     IKARGITTIEGEGRLISPTTVQVGDDTVTGKNVVLATGSYSRSLPGLEIGGRVITSEQ
                     ALELDFIPKKVAVLGGGVIGVEFSSVWRSWGSEVTIIEALPHLVPNEDEAISKQFERA
                     FRKRGIDFKLGVRFQGVTQNDQGVVVTLENGETIEAELLLVAVGRGPLTAGLGYEEVG
                     ITIDRGFVITNERLATNIPGVYAVGDIVPGLQLAHRGFQHGIFVAEEIAGLNPIVIDD
                     LNIPKVTYSDPEVASVGLSEAKAAEKYGADKITSYDYNLAGNGKSHIIGTSGSVKVVR
                     VNEGPVVGVHMIGARVGELIGEAQLAVNWEAYPEDIAPLLHAHPTQNEALGEAFLALA
                     GKPLHGI"
     gene            complement(1958..>2442)
                     /locus_tag="PLW15_11160"
     CDS             complement(1958..>2442)
                     /locus_tag="PLW15_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT34992.1"
                     /translation="GRLVLADGLVAASEEEPDAIIDVATLTGAALIALGNRYVGTMGD
                     SQLVADVVAAGNRVGETLWPMPIPSDVRPMLASDIADIANIKPGNTAAGMLIAAAFLR
                     DFVGSVGEGEAKTVIPWAHLDIAGPANNGAGGWGFTGKGPTGVSVRALIEFATGLGAK
                     "
BASE COUNT          378 a          777 c          842 g          445 t
ORIGIN      
        1 cgtttcgaca acgggtgcgg gcgcaggtgc ctcgacgact ggtgcgggtg cgggtgcctc
       61 gacgactggt gcgggtgcgg gtgcctcgac gactggtgcg gatgcctcag ccacgggctc
      121 cggtgcgggg gccggtgcct cggcgacggg tgccggagcg gcgggtgcct ccgtcgcgcc
      181 cgatccgtca ccgatggtga cgagggtggt accgacctcg accgtctcgt cctcggcgac
      241 gaggacggct tcgatcactc cggcgacagg ggacgggatc tccgtgtcga ccttgtcggt
      301 cgagacttcg agcagcggct cgtcgacctc gacccggtcg ccgaccttct tgagccagcg
      361 tgtcaccgtg ccctcggtga cactctctcc gagtgccgga aggttgacgg attcgctcat
      421 cagtgatgct ccttagtgcc gcgtacgtac ttagtttagt aaaggggttt cgcgccgggg
      481 tcagatgccg tgaagcggct tgcccgcgag agccaggaac gcctcgccga gcgcctcgtt
      541 ctgggtcggg tgcgcgtgaa gaagcggcgc gatgtcctcc gggtaggcct cccagttgac
      601 cgcgagctga gcctcgccga tcagttcgcc cactcgagcg ccgatcatgt gaactccgac
      661 gaccggtccc tcgttgaccc gcacgacctt gacggatccg gaggttccga tgatgtggct
      721 cttgccgttg cccgcgaggt tgtagtcgta gctcgtgatc ttgtcggcgc cgtacttctc
      781 tgcagccttg gcctcggaga gaccgacgga tgcgacctcc gggtcgctgt aggtgacctt
      841 cggaatgttg aggtcgtcga tgacgatcgg gttgagtccg gcgatctcct cagccacgaa
      901 gatcccgtgc tggaatccgc ggtgggccag ctgcaggccg ggcacgatgt cgcccacggc
      961 gtagacaccg gggatgttgg tggcgaggcg ttcgttggtg atgacgaagc cgcggtcgat
     1021 ggtgatgccg acctcctcgt agccgagtcc ggcggtgagc gggccgcgac cgacggccac
     1081 gagcaggagc tcggcctcga tggtctcgcc gttctccagg gtgacgacga cgccctggtc
     1141 gttctgagtc acgccttgga agcgaactcc cagcttgaag tcgattccgc gcttgcggaa
     1201 cgcacgctcg aactgcttcg agatcgcctc gtcctcgttg ggcacgaggt gggggagcgc
     1261 ctcgatgatc gtgacctcgg atccccagct gcgccagacg gacgagaact cgacgccgat
     1321 gacaccgccg ccgagaacgg cgaccttctt cgggatgaag tcgagctcga gtgcctgctc
     1381 ggaggtgatg acccgtccgc cgatctcgag accggggagc gagcgggagt agctgccggt
     1441 cgcgagcacg acgttcttgc cggtgaccgt gtcatccccg acctgcacgg tggtggggga
     1501 gatcagacgt ccctcgccct cgatcgtggt gatgccgcga gccttgatga ggccctgcag
     1561 tcccttgtac ttgctggcga caacctcctg gcggtagccg gtcaccgcgg cgatgtcgac
     1621 gccgttgaag gtggatgaga caccgtactt cgcggactcg cgcgtgacgt cggcgacctc
     1681 ggcggagtgc agcagcgcct tggtcggaat gcatccgacg tgcaggcagg tgccccccac
     1741 cttgttcttc tcgatgagac caacgctcat tccgagctgg cttgcgcgca cggcagcggc
     1801 gtagccacca cttccgccac cgagaactac gaggtcaaaa ttgtgctcag ccaaaaccag
     1861 caactcctct gtaaggatct tcaagcaccg gcgcctgatg gtgtggtgga cgaccggctg
     1921 gggcggtgag atcccgcctc gaaaacccta ctacttccta cttggcaccg aggccggtcg
     1981 cgaactcgat cagagcccgt acggagacgc cggtcgggcc cttgcccgtg aagccccaac
     2041 cgccggcgcc gttgttcgcc ggaccggcga tgtcgaggtg cgcccacggg atcacggtct
     2101 tggcctcgcc ctcgccgacg ctgccgacga agtcgcggag gaaggcggcg gcgatcagca
     2161 ttccggctgc ggtgttgccg ggcttaatgt tggcgatgtc ggcgatgtcc gacgcgagca
     2221 tgggtctcac atcgctgggg atcggcatgg gccacagggt ctcgccgacc cgattgccgg
     2281 cagcgaccac atcggcgacg agctgcgagt cgcccatggt gccgacgtag cggttgccca
     2341 gggcgatgag tgccgcaccg gtgagagtcg cgacgtcgat gatcgcatcg ggctcctcct
     2401 cgctcgcggc gacaaggccg tcagcgagca cgagccggcc tt
//
LOCUS       DAOSOU010000267         2330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_45907_length_2330_cov_5.640000, whole genome shotgun sequence.
ACCESSION   DAOSOU010000267 DAOSOU010000000
VERSION     DAOSOU010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45907_length_2330_cov_5.640000"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLW15_11165"
     CDS             complement(<1..528)
                     /locus_tag="PLW15_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT34993.1"
                     /translation="MAEKPRDSNPTDSEAVPDDAAPATPSRRAFLKTGAVGLGGLAVG
                     AAGGAAVTAAVTSQQASPFDPLAPRSEPGFDHIVAIMYENRSFDNILGFLYTDDDLAS
                     GQTFDGLAQGSYSNVGPAGEMIEAHVYIGGTDEVMRHPQPDPGEHYPHVNTQIFNIVD
                     PETNAKLFENELREPY"
     gene            complement(538..1986)
                     /locus_tag="PLW15_11170"
     CDS             complement(538..1986)
                     /locus_tag="PLW15_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT34994.1"
                     /translation="MSLTASFASTATAVVALELADAAALAALTDDTLLAAHALLTDHR
                     RHAETIAAHLAGEIARRSSRDDGYAGLAQRHGFGSASGLLQNLSPVTATEATQLIAAG
                     SLMAEPPSTLWEGALADALTSGLVSVAGADAIRRGLAPVADTAPADELLAECRRLLTR
                     VTQISIDELRREARSARDRIDEAGIARREKQRRDLRYLRRWIREDGMYQGAFLLDPEA
                     GLHLFTALDAIIAPRRGVRFVDAAAQAEADAVLADPRTDDQLLADGLTDMVRLAVDAD
                     PGSLFGTRRPAVRVIVTEDTLLTNGGHGYLEGDVQPVSRNTVDRHLCDTGMIGIKFDR
                     RGQVLNLGRTQRLFTEAQRLAISVRDGGCLFPRCTRPPAGCEVHHITPWSQGGSTDTA
                     DGVTLCRHHHMLLHNNQWSIHRDRHRYWLTPPPTHDPDRRPIPLVTKSLIMHELQRVS
                     SAAGGNGIDSRQLVGVGDAGNATRNSGETILT"
     gene            complement(2149..>2330)
                     /locus_tag="PLW15_11175"
     CDS             complement(2149..>2330)
                     /locus_tag="PLW15_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT34995.1"
                     /translation="NFIITVTFPSLSAASLPLTYAGYAVFAALSFFFILRVVPETKGM
                     KLEDADELLATRAAS"
BASE COUNT          376 a          708 c          841 g          405 t
ORIGIN      
        1 gtagggctcg cgaagctcgt tctcgaagag cttcgcgttc gtctcggggt cgacgatgtt
       61 gaagatctgg gtgttcacat gcgggtagtg ctcgccggga tcgggctgcg gatgacgcat
      121 cacctcgtcg gtgccgccga tgtagacgtg cgcttcgatc atctctccgg cggggccgac
      181 gttggagtag ctgccctgcg cgagaccgtc gaaggtctga ccgctggcca gatcgtcgtc
      241 ggtgtacagg aagccgagga tgttgtcgaa ggatcggttc tcgtacatga tcgcgacgat
      301 gtgatcgaat ccaggttcgc tgcgtggggc cagggggtcg aacggggatg cttgctggct
      361 cgtcaccgca gccgtgactg ccgcgcctcc cgcggcaccg acggcgagtc cgccgagccc
      421 gacggcaccg gtcttcagga atgcgcggcg cgacggggtg gccggggccg cgtcatccgg
      481 cacggcttcg gagtcggtcg ggttggagtc acggggtttc tcggccacaa aatcactcta
      541 ggtgaggatc gtttcgccgg agtttcgggt cgcgttgcca gcatcgccga cgccaacgag
      601 ctggcgcgag tcgattccat tcccgcccgc cgcgctactg accctctgca gctcgtgcat
      661 gatgagactc ttcgtgacca atggaatagg tcgtcggtcg gggtcgtgcg tcggcggtgg
      721 tgtgagccag taccggtgcc ggtcgcggtg aatgctccac tggttgttgt ggagcagcat
      781 gtggtggtgg cggcacagcg tgactccgtc agcggtgtcg gtgctgcctc cctgcgacca
      841 cggggtgatg tggtggactt cgcagcccgc tggtggtcgg gtgcagcgcg ggaagagaca
      901 tccgccgtct cggactgaga tggccagtct ctgagcttcg gtgaagagtc gttgagtgcg
      961 accgaggttg aggacctgcc cgcggcggtc gaacttgatg ccgatcatgc cggtgtcgca
     1021 caggtgtcgg tcgacggtgt tgcgtgacac aggctgcaca tcgccttcaa ggtatccgtg
     1081 accgccattc gtaagcagcg tgtcctcggt gacgatgacc cgcaccgcgg gccgacgggt
     1141 accgaacagc gacccggggt cggcgtcgac ggcgaggcgc accatgtcgg tgaggccgtc
     1201 ggccagcagt tggtcgtcgg tgcgggggtc ggcgagcacg gcgtcggcct cggcctgagc
     1261 tgcagcatcc acgaaccgca ccccgcggcg cggtgcgatt atcgcgtcga gcgccgtgaa
     1321 caggtggaga cccgcttcgg ggtcgagaag gaacgcaccc tgatacatgc cgtcctcgcg
     1381 gatccaccgt ctgaggtagc gcaggtcgcg ccgctgcttc tcacggcggg cgataccggc
     1441 ctcgtcgatg cggtcacgcg cggatcgagc ctcacggcgt agctcgtcga ttgatatctg
     1501 ggtcactcgc gtcagcagcc gacggcactc ggccagcagc tcgtcggccg gcgccgtatc
     1561 agcaaccggt gcgagtcccc gtcggatcgc gtcggcaccc gccaccgaca cgagaccaga
     1621 ggtcaaggcg tcagccaggg cgccctccca gagtgtggac ggcggctcgg ccatcaacga
     1681 gcccgccgcg atcagctggg tcgcctcggt cgctgtcacc ggcgacaggt tctggagcag
     1741 cccgctagcg ctcccgaacc cgtggcgctg cgccaatccc gcatagccgt catcgcggga
     1801 ggaccgccgg gcgatctccc cagcgaggtg cgcagcgatc gtctccgcgt ggcgacggtg
     1861 atcggtgagc agggcgtgtg cggccaggag tgtgtcgtcg gtgagggctg ccagagccgc
     1921 ggcatccgcc agctcgagcg cgacgaccgc agtggcggtg gaggcgaacg aggcagtcag
     1981 tgacatgcct ccatcctctc atattcgaac gtatgttcga agggaaaagc ccggggaatc
     2041 agcaactttt tgagatcagg ggtgtggagg agaggagcaa tggggtgggt agcgtcgggt
     2101 ttcgatacgc cgccgatggc ggctactcaa ccagcgggag gcggccactc aactggcggc
     2161 gcgagtcgcc agcagctcgt cggcatcctc gagcttcatg cccttggtct cgggcacgac
     2221 ccgcaggatg aagaagaacg acagcgcagc gaacaccgcg tagcccgcgt aggtcagcgg
     2281 cagcgacgcg gccgacagcg acgggaacgt gaccgtgatg atgaagttcg
//
LOCUS       DAOSOU010000268         2303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_46659_length_2303_cov_5.545374, whole genome shotgun sequence.
ACCESSION   DAOSOU010000268 DAOSOU010000000
VERSION     DAOSOU010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46659_length_2303_cov_5.545374"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1002)
                     /gene="ccsB"
                     /locus_tag="PLW15_11180"
     CDS             complement(46..1002)
                     /gene="ccsB"
                     /locus_tag="PLW15_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOT34996.1"
                     /translation="MTETLVMYMRLALYSAMAVYTLAFILFTVDLARRSAGAPVAATV
                     GSKAVAASGTTAVLEKTDAPASVGRSTPRYLRAAMALTVIAWVLHVTAAILRGLAAGR
                     VPWANMFEFTLTATAIIVGVFLAVQFWQDLRFLGAFISGFVLIALGVATVNFYVDVVP
                     LPPALQSAWLVIHVFVATLGTGFFALGAGLSIVQLLKERRAVGKLKAAKFLDTLPEPE
                     KLENMAYRLIVVGFVLWTFTLIAGAIWASRAWGRYWGWDTKEVWTFIIWTIFAGYIHA
                     RATRGWRGSRSAWLAIIGFSAVLFNFTIVNLFFKGLHAYSGL"
     gene            complement(1012..>2303)
                     /locus_tag="PLW15_11185"
     CDS             complement(1012..>2303)
                     /locus_tag="PLW15_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOT34997.1"
                     /translation="LFVSLIGCVIPRTRHHLNALRAKPPATPARLARLEGFTTRGTTV
                     DAATAVAAAETVLRKQGYRTELYPAGPGQAATSVSAERGYLRETGNLVFHTALIGVLV
                     SVGIGGGFGFTGQRVVVEGQAFVNSLASYDSFNPGRFFSESELEPFTIVLDSFDVSYE
                     EQNLKALGQAVDFTANVTTTLRGQEPAPATIKVNEPLSIGGTQVYLLGNGYAPVVTVR
                     DPEGAVVFSQPVAFLPQDANLTSLGIIKVPDGLSEQLGMRGFLYPTAVALPSGALTSN
                     YPDLLNPILTLEVYQGDLGLDNGQGVNAYSLNTDSLTQIAGRKSDAPTIELSPGDKVD
                     LPGDLGSIELESIPRFVSLDVHHDPSQLWVLIFSALVVAGLVTSLFIPRRRMWVKAVT
                     TKDGVTLEYAGLARGDDPTLTAAVADLADRHIEQLKA"
BASE COUNT          397 a          736 c          817 g          353 t
ORIGIN      
        1 gggtttcgat acgcgccttc ggcgctactc aaccggcctg gggcactaca gcccgctgta
       61 cgcgtgcagc cccttgaaga acaggttcac gatcgtgaag ttgaagagca cggcgctgaa
      121 gccgatgatc gcgagccagg cgctgcgcga gccgcgccat ccgcgggtcg ccctcgcgtg
      181 gatgtagccg gcgaagatcg tccagatgat gaaggtccag acctccttcg tgtcccagcc
      241 ccagtagcgg ccccacgccc gtgaggccca gatggcgccg gcgatgagcg tgaaggtcca
      301 cagcacgaac ccgacgacga tgagccggta ggccatgttc tcgagcttct cgggctcggg
      361 cagcgtgtcg aggaacttcg ccgccttcag cttgccgacc gcgcgacgct ccttgagcag
      421 ctgcacgatc gacaggccgg cgccgagagc gaagaacccg gtacccaggg tcgcgacgaa
      481 cacgtggatg accagccacg ccgactgcag tgccggcgga agcggcacga cgtcgacgta
      541 gaagttgacc gtcgcgactc ccagagcgat cagcacgaag ccggagatga acgcgccgag
      601 gaagcgcaga tcctgccaga actgcaccgc gaggaacacc ccgacgatga tcgccgtcgc
      661 cgtgagcgtg aactcgaaca tgttggccca cggcacgcgg ccggcggcga gtccgcgcag
      721 gatcgcggcc gtgacgtgca gaacccaggc gatgacggtg agcgccatgg ccgcgcgcag
      781 gtagcggggg gtgctgcggc cgacggaagc cggggcatcc gtcttctcca gaacggcagt
      841 ggttccggat gccgctaccg ccttgctccc gacggtcgcc gccaccggcg cgccagccga
      901 ccgtcgcgca agatcgacgg tgaagaggat gaaggccagc gtgtagaccg ccatggccga
      961 gtacagggcc aggcgcatgt acatcacgag agtctcggtc acggttccac cctaagcctt
     1021 gagttgttcg atgtgccggt cggccaggtc ggccaccgca gcggtgagtg tggggtcgtc
     1081 gccccgggcg agtccggcgt actccagcgt gacaccgtct ttggtggtta cggccttcac
     1141 ccacatgcga cgacgcggaa tgaacaggct ggtgaccagt cctgcgacaa cgagcgccga
     1201 gaagatcagc acccacagct gcgacgggtc gtggtgcacg tcgagcgaca cgaaccgcgg
     1261 gatcgactcg agttcgatgc ttcccaggtc ccccgggagg tcgaccttgt ctccggggct
     1321 gagttcgatc gtgggcgcgt ccgacttgcg cccggcgatc tgggtgagcg agtcggtgtt
     1381 gagcgagtac gcgttcacgc cctgcccgtt gtcgagcccc agatcgccct ggtacacctc
     1441 gagggtgagg atcgggttga gcaggtcggg gtagttcgag gtgagcgccc ccgatggcag
     1501 tgccaccgcg gtcgggtaga ggaagccccg catacccagc tgctcgctca ggccgtcggg
     1561 caccttgatg atgccgaggc tggtcaggtt ggcatcctgg ggcaggaaag cgacgggctg
     1621 cgagaagacg acggcgcctt cggggtcgcg caccgtcacc accggcgcgt atccgttgcc
     1681 gagaaggtag acctgcgtgc cgccgatcga gaggggctcg ttgaccttga tggtcgccgg
     1741 cgccggttcc tgcccgcgca gggttgtcgt cacgttggcg gtgaagtcga cggcctgccc
     1801 gagggccttg aggttctgct cctcgtagct gacatcgaag ctgtcgagca cgatggtgaa
     1861 cggctccagc tcggattcgc tgaagaagcg ccccgggttg aacgagtcgt agctggcgag
     1921 ggagttgacg aaggcctgac cttcaaccac gacgcgctgg ccggtgaagc cgaagccgcc
     1981 gccgatgccg acgctcacca gcactccgat gagcgcggtg tggaacacca ggttgccggt
     2041 ctcgcgcagg tatccgcgct cggcgctgac cgaggtggcc gcctgccctg ggcctgccgg
     2101 gtacagctcg gtgcggtaac cctgcttgcg cagcaccgtc tcggcggccg ctacggcggt
     2161 cgcggcatcc acagtcgtcc cgcgggtggt gaagccctcg aggcgagcga gtcgggccgg
     2221 cgtggccggc ggcttcgcgc gcagcgcatt gaggtggtgc cgggtgcgcg ggatgacgca
     2281 gccgatgagc gagacgaaca gca
//
LOCUS       DAOSOU010000269         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_47692_length_2266_cov_5.627770, whole genome shotgun sequence.
ACCESSION   DAOSOU010000269 DAOSOU010000000
VERSION     DAOSOU010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47692_length_2266_cov_5.627770"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLW15_11190"
     CDS             complement(<1..910)
                     /locus_tag="PLW15_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOT34998.1"
                     /translation="MPLTRRQIYRRRRTVVFGGAAVALATAFYLPITLLAPLHDAVPV
                     ELSVATPAVEQPTLDFPEYGASGLGAIGYPGVLAQGGVTDPLPIASISKVVTALVVLD
                     AKPLAPGESGPDLTFSSVDEDFYNEQVAQNGVVASVSAGQVMSQRNVMSLMLMASANN
                     YAQSLAAWAFGSEAAYVDAARDWLARQGLTGTTIADATGINPANASTVPDLITLAKLA
                     IADPVVSEIVGTANLEIPDIGLVENRNGLLGVDGIDGIKTGTLDEAGSCLLFSAEHTV
                     GASTVTLVGVVLGGPDHSTINAAVRGL"
     gene            952..1833
                     /locus_tag="PLW15_11195"
     CDS             952..1833
                     /locus_tag="PLW15_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOT34999.1"
                     /translation="MERVAFESTAIELYASRSSWDATAADLVRTMLERWGLVPGAAFV
                     GGEAASVLHVTTGDGIEAVLKVGFPHVEAVGEAIALESWGPGLAPRVLRQDPWTWSML
                     LERLRPGTSLRESSLPTLERVRIGSDLLRRISATTAPPGIPTVTEIAGHYLSDAVARL
                     DDQHSALVALGALDDVEHGLAVLENLAASDSGTGFVHGDLNPNNVLESQGRWMVIDPK
                     PMAGGPEFDVWPLVTQLGDLRDLEHRLAETGFTADRTVLWGIARSALDVTWAVMDSRP
                     ATEAVRVLRAWMRLSGA"
     gene            complement(1820..>2266)
                     /locus_tag="PLW15_11200"
     CDS             complement(1820..>2266)
                     /locus_tag="PLW15_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT35000.1"
                     /translation="VVVLEDIVDHTNVGAIFRSVAGLGADAVLVTPRCADPLYRRSVR
                     VSMGTVLQVPWTRLPDWPLGAEQLHAAGFHIAALALSDTAVELDDFAAAAPERVALIL
                     GTEGDGLSPDALSAADTVVTIPMLHGVDSLNVAAASAVALYALRRR"
BASE COUNT          367 a          733 c          803 g          363 t
ORIGIN      
        1 gaagtccgcg aacggcggcg ttgatggtcg agtggtcggg cccgccaagc accactccga
       61 ccagagtgac ggtcgaggcg ccgacggtgt gctcggccga gaacaggagg cacgaccccg
      121 cttcatccag ggttcccgtc ttgatgccgt cgatgccgtc gacgccgagc agaccgttgc
      181 ggttctcgac gagaccgatg tcggggatct cgaggttcgc ggtgccgacg atctcggaca
      241 ccacggggtc ggcgatggca agcttcgcga gggtgatgag atcgggcaca gtgctggcgt
      301 tcgcaggatt gatgcccgtc gcatccgcaa tggtggtgcc ggtcagaccc tgacgagcga
      361 gccagtccct cgcggcatcc acgtaggcgg cctcggagcc gaatgcccac gcagcgagcg
      421 attgagcgta gttgttggcc gacgccatca gcatgagact catgacattg cgctgggaca
      481 tcacctggcc ggcgctcacc gacgcgacaa ctccgttctg ggcgacctgt tcgttgtaga
      541 agtcctcgtc gacactcgag aaggtgagat cggggccgga ctcccccggt gccaaaggct
      601 tggcatccag aacgacgaga gccgtgacga ccttcgagat cgacgcgatc ggcagcgggt
      661 cggtgactcc gccctgtgcg agaacccccg ggtacccgat tgctcccagg cccgatgcac
      721 cgtattccgg gaagtccagg gtcggctgct ccaccgccgg tgtcgcgacg gacagctcga
      781 cgggaaccgc gtcgtgcagc ggcgcgagga gcgtgatcgg aaggtagaac gcggtggcca
      841 gcgccactgc ggcaccgccg aacacgacgg tgcgacggcg ccggtagatc tggcgtcggg
      901 tcagaggcat ccgtcaatcg taaccggggg ctcgcggcgt atcgtcgacg catggagcgc
      961 gtcgcattcg agtcgacagc gatcgagctg tatgccagca ggtcgagctg ggatgccacg
     1021 gccgccgatc tcgtgcgcac gatgctcgag cgctgggggt tggtgcccgg cgccgccttc
     1081 gtcggaggtg aggccgcgag tgtcctccac gtcaccacgg gtgacggcat cgaagcggta
     1141 ctcaaggtgg gatttcccca cgtcgaggct gtgggtgagg cgatcgccct cgagtcgtgg
     1201 ggccctggcc tcgcaccccg ggtgctgcgt caggatccgt ggacgtggag catgctgctc
     1261 gagcgcctgc gccccggcac gtcgttgcgc gagtcgtccc tgccgacact cgagcgcgtg
     1321 aggatcggct ccgacctgct gcgccgcatc tcggcgacga ccgcgccacc ggggataccg
     1381 accgtgaccg agatcgcggg gcactacctc tcggacgccg tggcgcggct ggatgaccag
     1441 cactcggcgc tggtcgcgct cggtgctctc gatgatgtgg agcacggtct cgctgtgctc
     1501 gagaacctgg cggcaagtga ctcgggcacc ggcttcgtac atggcgacct gaaccccaac
     1561 aatgtgctcg agtcgcaggg ccgctggatg gtcatcgacc ccaagccgat ggcaggcggg
     1621 cccgagttcg atgtgtggcc gctcgtgacc cagttgggcg atctccgcga cttggagcat
     1681 cgcctggccg agaccggttt caccgccgac agaacggtgc tgtggggcat tgcccgcagt
     1741 gcgctcgacg tcacgtgggc ggtcatggat tcgcgcccgg caacggaggc cgttcgcgtt
     1801 ctgcgagcct ggatgcgact cagcggcgcc tgagcgcgta cagggcgacc gcgctcgctg
     1861 cggcgacatt gagcgagtcg actccgtgca gcatggggat cgtcaccacc gtgtcggcgg
     1921 cggagagtgc gtcggggctc agaccgtcac cctcggtgcc gaggatcagc gcaacccgtt
     1981 ccggtgctgc ggcagcgaag tcgtccaact cgactgcagt atcggatagc gcgagcgccg
     2041 cgatgtggaa accggcggcg tggagttgct cggcgccgag cggccagtcg ggcagacggg
     2101 tccacggcac ctggagcacc gtgcccatgc tcactcgcac gctgcggcgg tacagcggat
     2161 ctgcgcaccg cggtgtcacc agcaccgcat cggcgcccag ccccgcgacc gagcggaaga
     2221 tcgctcccac gttggtgtgg tcgacgatgt cctcgagcac gaccac
//
LOCUS       DAOSOU010000270         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_49301_length_2210_cov_4.036659, whole genome shotgun sequence.
ACCESSION   DAOSOU010000270 DAOSOU010000000
VERSION     DAOSOU010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49301_length_2210_cov_4.036659"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1869
                     /locus_tag="PLW15_11205"
     CDS             148..1869
                     /locus_tag="PLW15_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HOT35001.1"
                     /translation="MFSLALIGFLGGLITGISPCILPVLPVIFLSGGALSARADGAPD
                     AAPTVSRWRPYLVISGLVVAFSVFTLIGSLLLALLNLPQDFLRWAGIVVLVLIGVGLI
                     VPKFQHILEKPFSWIPQKNVGTDRGGFVLGLALGAVYVPCAGPVLAAITVAGSTGRIG
                     PETIVLTVSFALGAALPLLVFALAGRRVAERVKTFRKHQQRIRVIGGVLMIALSIGLV
                     FNVPQLIQRLIPDYTSALQDQFSNSEQIAEQLDLGGLENEQNKDLDLCTNGATELESC
                     GTAPDITGIQQWFNTENDQPVDLTELKGKVVLVDFWAYSCINCQRSIPHITAWYDAYK
                     DAGLEVIGIHSPEYAFEKVPANVMAGARNFGITYPVALDNNLSTWTNYRNRYWPAHYL
                     IDAEGVVRHVKFGEGGYANTEELIRQLLVAANPDVKLPPATDVSDDTPETGSTTPETY
                     LSLGKVVNFGGDEKYASGTNSYRYPSTLADDSFALKGDWTLDFQNAQPANGPASIRLN
                     YTAREVRMVLGGEGTVTVTVNGEKREFDVSGTPNSYSIIPMGGAAKGTLEVTLSPGLE
                     AYSFTFG"
BASE COUNT          361 a          801 c          676 g          372 t
ORIGIN      
        1 gttcggaggt ttcgctcagg ttggcggccc tcggcggccg agttctgggc agttcggctg
       61 gctcagccaa ccgttcgacg ccaccgttcc gaactgcgtg agaccggcgg aaccccgccc
      121 gcctcgcctc acctcacgga ggacaacgtg ttcagcctcg ccctcatcgg attcctcggt
      181 ggcctcatca ccggaatctc gccctgcata ctcccggtgc tgccggtcat cttcctgtcg
      241 ggtggcgcgc tcagcgcccg cgccgatggg gctccggatg ccgcacccac cgtctcgcgc
      301 tggcgcccct acctcgtcat ctccggcctc gtggtggcgt tcagcgtctt caccctgatc
      361 ggatcgcttc ttctcgcgct gttgaatctg ccgcaggact tcctgcgctg ggcgggcatc
      421 gtggtgctgg tgctcatcgg cgtcggtctg atcgtgccga agttccagca cattctggag
      481 aagccgttct cgtggatccc gcagaagaac gtcggcaccg accgcggcgg attcgtgctc
      541 ggcctcgcgc tcggcgccgt gtacgtgccg tgcgcaggcc ccgtgctcgc ggcgatcact
      601 gtggccggct cgaccggccg catcggaccg gagacgatcg tgctcacggt gagcttcgcg
      661 ctcggtgccg cactgcccct gctcgtgttc gccctcgccg gccgccgtgt cgccgagcgg
      721 gtgaagacct tccgcaagca ccagcagcgc atccgggtca tcggcggggt gctcatgatc
      781 gccctgtcga tcggcctggt gttcaacgtg ccgcagctca tccagcggct catccccgac
      841 tacacgagtg ccctccagga tcagttctcc aactcggagc agatcgctga gcagctcgac
      901 ctgggcggcc tcgagaatga gcagaacaag gatctcgacc tctgcaccaa cggcgccacc
      961 gagctcgaga gctgcggcac cgcccccgac atcacgggca tccagcagtg gttcaacacc
     1021 gagaacgacc agccggtcga cctgaccgag ctcaagggca aggtcgtgct cgtcgacttc
     1081 tgggcctact cctgcatcaa ctgccagcgc tcgattccgc acatcaccgc ttggtacgac
     1141 gcctacaagg atgccggact cgaggtcatc ggcatccact cgcccgagta cgccttcgag
     1201 aaggtgcccg ccaacgtcat ggcgggggcg cgcaacttcg gcatcacgta tcccgtggcc
     1261 ctcgacaaca acctcagcac ctggaccaac taccgcaacc gctactggcc cgcgcactac
     1321 ctgatcgatg ccgagggcgt cgtgcgccac gtgaagttcg gcgagggcgg ctacgccaac
     1381 accgaggagc tcatccgcca gttgctggtc gcggccaacc ccgacgtgaa actgcccccg
     1441 gcaacggatg tctcggacga cacccccgag acgggctcca ccaccccgga gacctacctc
     1501 agcctcggca aggtggtgaa cttcggcggc gacgagaagt acgcgtcggg cacgaacagc
     1561 tatcgctacc cgtcgaccct ggccgacgac tcgttcgcgc tgaagggtga ctggacgctc
     1621 gacttccaga acgcccagcc ggcgaacggg cccgcgagca tccggctcaa ctacaccgcc
     1681 cgcgaggtgc ggatggtgct gggcggcgag ggcacggtga ccgtcacggt caacggtgag
     1741 aagcgcgagt tcgacgtgtc gggcactccc aactcgtact cgatcatccc gatgggtggg
     1801 gctgccaagg gcacccttga ggtgacgctg tcgccagggc tcgaggccta ctcgttcacc
     1861 ttcgggtagg cggcatcctt ccctcgtccc aaatgcagga gattcttgag ctgccagccg
     1921 gccgtcgggc ctccggagcg catcaccaag aaatctcctg catttgggcg gcgaccgacg
     1981 cctcgcgtga cgggaccatc ggcactgacc gccgcaccgc ccgcgagcca cactgggcac
     2041 atgatcgcac cgaccgtcta ccgcgtgact gcgcgcacgg catcagaccg cgcgtcaagc
     2101 atcaccgctg gcgacaccgt catccccctt gactcggcct ggcagcccgc gtcgccatcg
     2161 ggactgccag gccccgcaga gctgctgtcg gctgcattgg ccggatgcca
//
LOCUS       DAOSOU010000271         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_52027_length_2126_cov_9.225012, whole genome shotgun sequence.
ACCESSION   DAOSOU010000271 DAOSOU010000000
VERSION     DAOSOU010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52027_length_2126_cov_9.225012"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..484)
                     /locus_tag="PLW15_11210"
     CDS             complement(170..484)
                     /locus_tag="PLW15_11210"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT35002.1"
                     /translation="MSDTQPEAQLTSLRQSIDNIDAALVHLLAERFKFTQQVGRLKAA
                     TGMPPSDAERERVQIARLRALAEESHLDPAFAEKFLNFIVAEVIHHHEQIASTGTIET
                     QP"
     gene            complement(513..1148)
                     /locus_tag="PLW15_11215"
     CDS             complement(513..1148)
                     /locus_tag="PLW15_11215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT35003.1"
                     /translation="MPAKRPEPRVIEGRVIRLEPLLDEHLPELYAAIGAPEVFAGGWG
                     GGPAGHQASEADFVEFARGYFAWGRNNVYAVRLASGPDAAALVGTSTLGDFDEANEHA
                     HIGWTAYSPQVWATAVNPEAKLLMLGEAFDHGFGRVKLQADALNDRSRAAILKLGAKF
                     EGIVRRDRLRADGTWRDTAVYSILIDEWPAVRVGLEERLAAFGDETPSLRK"
     gene            1171..1794
                     /locus_tag="PLW15_11220"
     CDS             1171..1794
                     /locus_tag="PLW15_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35004.1"
                     /translation="MTGSWRDSPLVRIVGSAASWFLFSLSFTLLYLVTFVVMALGGSC
                     ASGGPYDIAVQCPDNVTLFAPLSIFGGLIAVGISIALAQGFGTPLTTWAWPVLFVGLG
                     TAFLAAFIFGQDIVGLIIGAFFVLMGLTPLVLELRGSPQRVFLGQFAPNGAQYYEGEN
                     ARRSLMSPRAPNPDTAILPSAGSWILSLGIAVIAVFAGYLTALAWFS"
     gene            complement(1791..>2126)
                     /locus_tag="PLW15_11225"
     CDS             complement(1791..>2126)
                     /locus_tag="PLW15_11225"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOT35005.1"
                     /translation="RINGVTDFVMTKLDVLTGLKAIPVCVAYDIDGVRVEEVPVSQSD
                     FHHAVPIYQEFPGWDEDISGVRRFEDLPKTAQEYVLAIEAMSGSRISAIGVGPDREAI
                     VVRHDLVNG"
BASE COUNT          295 a          739 c          713 g          379 t
ORIGIN      
        1 cgccgcaggg actcgagtgc tctggtcgcg tcgccgcgcg tggtgatgat cacgcgggct
       61 ccggccaccg ccagctgctc ggccgtgaag tagcccacac cgcgtcccgc accggtgacg
      121 acaatcgttc gacccgcctg tgaggggagg gtcgtcggat cccagccgct cacggctggg
      181 tctcgatggt gcccgtgctg gcgatctgct cgtggtggtg gatgacttcg gcgacgatga
      241 agttcaggaa cttctccgcg aacgccgggt cgaggtgcga ttcctcggcg agggcccgca
      301 gtcgggcgat ctgcacgcgc tcgcgctcgg catcggaggg cggcatcccg gtggcggcct
      361 tcagcctgcc gacctgctgc gtgaacttga agcgctcggc cagaaggtgc acgagggccg
      421 cgtcgatgtt gtcgatgctc tggcgcaggc tcgtgagttg ggcctcgggc tgcgtgtcgc
      481 tcatgccgac aatctaccgg tggtgcgctg tgctatttcc gcagcgacgg ggtctcgtcg
      541 ccgaaggccg cgagccgctc ctcaagcccc acccgcacgg cgggccattc gtcgatgagg
      601 atcgagtaca cggcggtgtc gcgccaggtg ccatcggcgc gaaggcggtc gcgccgcacg
      661 atcccctcga acttcgcgcc gagcttgagg atggccgcgc gggagcgatc gttgagtgcg
      721 tcggcctgca gcttcacgcg cccgaacccg tgatcgaagg cctcaccgag catgagcagt
      781 ttcgcctccg ggttcaccgc ggtggcccac acctgcggtg agtacgccgt ccagccgatg
      841 tgcgcgtgct cgttggcctc gtcgaagtcg ccgagagtcg acgtgccgac gagagccgcg
      901 gcatccggcc cgctggcgag cctgacggcg tagacgttgt tcctgcccca ggcgaagtag
      961 ccgcgggcga actcgacgaa gtcggcctcg ctcgcctgat gtccggcggg gccaccaccc
     1021 cagccgccgg cgaagacctc gggggcgccg atggccgcat acagctcggg caggtgctcg
     1081 tcgagaagcg gttccagacg gatgacacgc ccctcgatca cgcggggttc gggtcgcttc
     1141 gccggcaccc gtccacacta cagttgcacc atgaccggat cgtggcgcga ttccccgctc
     1201 gtgcgtatcg tcggctccgc cgcctcctgg ttcctcttct cgctgagctt caccctgctg
     1261 tacctggtca cgttcgtggt gatggccctc ggcgggtcgt gcgcgagcgg cggcccctac
     1321 gacatcgcgg tgcagtgtcc cgacaacgtg accctgttcg cgccgctcag catcttcgga
     1381 ggcctcatcg cggtaggcat ctcgatcgct ctcgcgcagg gcttcggcac tccgctcacc
     1441 acctgggcgt ggccggtgct gttcgtcggc ctcggcacgg cgttcctcgc agctttcatc
     1501 ttcggtcagg acatcgtcgg cctgatcatc ggagcgttct tcgtgctgat gggcctcacg
     1561 cccctggtgc tggagttgcg cggcagcccg cagcgcgtct tcctcggtca gttcgcgccg
     1621 aacggggctc agtactacga gggcgagaac gcgcggcgct ctctcatgtc gccgcgagcg
     1681 ccgaacccgg acacggcgat cctgccgagc gccggaagtt ggatcctgtc gctgggcatc
     1741 gcggtgatcg ccgtcttcgc cggctacctg accgcgctgg cctggttcag ctagccgttc
     1801 accagatcgt ggcgcaccac gatcgcctcg cggtcgggcc ccacgccgat cgctgagatg
     1861 cgcgaacccg acatcgcctc gatggcgagc acgtactcct gcgcggtctt cggcaagtcc
     1921 tcgaagcggc gcacgcccga gatgtcctcg tcccagcccg ggaactcctg gtagatcggc
     1981 acggcgtggt ggaagtccga ctgcgacacc ggaacctcct cgacgcgcac gccgtcgatg
     2041 tcgtacgcga cgcacaccgg aatcgccttc aacccggtga gcacgtcgag cttggtcatc
     2101 acgaagtcgg tcaccccgtt gatgcg
//
LOCUS       DAOSOU010000272         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_52131_length_2123_cov_6.407157, whole genome shotgun sequence.
ACCESSION   DAOSOU010000272 DAOSOU010000000
VERSION     DAOSOU010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52131_length_2123_cov_6.407157"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLW15_11230"
     CDS             <1..946
                     /locus_tag="PLW15_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT35006.1"
                     /translation="YHNGPLSWTLLSLLAGNHVVVTDRFDAARTLALLEEHRANMVYL
                     VPTMMKRISRLPAEVRESADLSALQVVWHLAEPCPAWLKGEWIEWLGPKRIWELYAGT
                     ENQASTVIRGDQWLEHRGSVGRVSSGEMAVFDADGAALPPGEVGEIFMRRTPGLAPSY
                     RYVGAEPRTIGDGWESLGDMGWMDADGFLYLADRKTDMILSGGANIYPAEIEAALNEH
                     PAVLSAAVIGLPDDDLGNRVHAIVQTDPDAIDDGTTDADDLRNHLGDRLVRYKVPRSF
                     EFVTEPLRDEAGKVRRSQLRADRVTPPPTSTPSQETQQ"
     gene            943..1431
                     /locus_tag="PLW15_11235"
     CDS             943..1431
                     /locus_tag="PLW15_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOT35007.1"
                     /translation="MSIYDHGIQALNGTDNLLGDVAGKTALFVNVASKCGLTPQYEQL
                     QALQDRFGSDDFTVVGFPCNQFLEQEPGSADEIAEFCSVNYGVTFPLSEKIDVNGDDK
                     HPLFADLEAAADVADGHTGDVRWNFEKFLVSADGEVVGRFGPTVTPDDPSIVSAIESQ
                     LA"
     gene            1554..>2123
                     /locus_tag="PLW15_11240"
     CDS             1554..>2123
                     /locus_tag="PLW15_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT35008.1"
                     /translation="MYEWSDEHLMLQQAMRDFIAKEILPIKEELEHGDLPPYDVLRKL
                     YKTFGMDEMARSTFAKRIAHEKAIEAGEPVEDKPSRHGGGDAGYTLIPIIELCRHCPG
                     MVTAMGVSVGLTASAIQSKGTIAQKERWVPELLTLEKVGAWAITEPNSGSDAFGSMLS
                     TARRDGDEYVLNGSKTFITNGPYADTIVFI"
BASE COUNT          379 a          737 c          682 g          325 t
ORIGIN      
        1 gtaccacaac ggtccgttgt cctggacgct gctcagcctg ctcgccggga accacgtcgt
       61 ggtcaccgac cgcttcgacg ccgcgcgaac gctcgcgctc ctcgaggagc accgggcgaa
      121 catggtgtac ctggtcccca cgatgatgaa gcgaatctcg cggctccccg ccgaggttcg
      181 cgagtccgcg gacctctcgg ccctccaggt cgtgtggcac ctcgccgagc cgtgcccggc
      241 ctggctcaag ggggagtgga tcgagtggct gggcccgaag cggatctggg agctgtacgc
      301 cggcaccgag aaccaggcgt cgaccgtgat ccgcggcgat cagtggctcg agcatcgcgg
      361 gtcggtcggg cgcgtgtcgt ccggcgagat ggccgtgttc gatgccgacg gcgcggccct
      421 tccacccgga gaagtcggcg agatcttcat gcgccgaact ccgggactcg ctccctcgta
      481 ccgctacgtc ggcgccgagc cccgcacgat cggcgatgga tgggaatcgc tcggtgacat
      541 ggggtggatg gacgccgacg gcttcctcta cctcgccgac cgcaagaccg acatgattct
      601 gagcggcggc gccaacatct accccgccga gatcgaggct gcgctcaacg agcatccggc
      661 tgtcctgtcc gcggcggtca tcggcctgcc cgacgacgac ctgggcaacc gggtgcacgc
      721 gatcgtccag accgaccccg acgcgatcga cgacggcacg accgatgccg acgatctgcg
      781 gaatcatctc ggcgatcgac tggttcgcta caaagtgcct cggtcgttcg agttcgtcac
      841 cgaaccgctg cgcgacgagg ccggtaaggt ccgccgcagc cagctgcgcg cagaccgcgt
      901 gacaccacca ccgacatcga ccccctccca ggagacccag caatgagcat ctacgaccac
      961 ggcatccagg cactcaacgg caccgacaat ctgctgggcg atgtggccgg caagacggcc
     1021 ctcttcgtca acgtcgcctc caagtgcggc ttgacccccc agtacgagca gctccaggcg
     1081 ctccaggatc gcttcggttc cgacgacttc accgtggtgg ggttcccgtg caaccagttc
     1141 ctcgagcagg agccgggcag cgcggacgag atcgccgagt tctgcagtgt gaactacggc
     1201 gtcacgttcc cgctgtcgga gaagatcgac gtcaacggcg acgacaagca cccgctgttc
     1261 gccgacctcg aagcggccgc cgacgtcgct gacggtcaca ccggtgacgt tcggtggaac
     1321 ttcgagaagt tcctcgtgtc cgcggacggc gaggtcgtcg gccggttcgg cccgaccgtg
     1381 acgcccgacg acccctcgat cgtctccgcc atcgagtcgc agctggcgta gccaacaacg
     1441 caactcggat cggccggggt accggagcga cagtagggag cggagcgacc gtacctagcg
     1501 aaggtggccc cggcctggga agttgactgg gcggtcaagt aacgttcgcc gccatgtacg
     1561 aatggtctga cgaacacctg atgctgcagc aggcgatgcg cgacttcatc gccaaggaga
     1621 tcctccccat caaggaggag ctcgagcacg gcgacctgcc gccgtacgac gtgctgcgca
     1681 agctgtacaa gacgttcggc atggacgaga tggcgcggtc gacgttcgcc aagcgcatcg
     1741 cccacgagaa ggcgatcgag gccggcgaac ccgtcgagga caagccgtcc cgccacggtg
     1801 gcggcgatgc cggctacacc ctcatcccga tcatcgagct gtgccgccac tgccccggca
     1861 tggtcacggc catgggcgtg tcggtcggtc tcacggcgtc ggcgatccag tccaagggca
     1921 cgatcgccca gaaggaacgt tgggtccccg agctgctcac cctcgagaag gtcggcgcgt
     1981 gggcgatcac cgaacccaac agcggctccg acgccttcgg ttcgatgctg tcgaccgccc
     2041 ggcgtgacgg cgacgagtac gtcctcaacg gctcgaagac cttcatcacc aacggcccct
     2101 acgccgacac catcgtgttc atc
//
LOCUS       DAOSOU010000273         2078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_53650_length_2078_cov_5.094909, whole genome shotgun sequence.
ACCESSION   DAOSOU010000273 DAOSOU010000000
VERSION     DAOSOU010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53650_length_2078_cov_5.094909"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLW15_11245"
     CDS             complement(<1..549)
                     /locus_tag="PLW15_11245"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOT35009.1"
                     /translation="MDVIATRDEYLEAVRTIAAGTGPIAIDAERASGYRYSQRAYLIQ
                     IFRRDAGTFLFDPPAIGGFTELNDVIAHEEWILHAASQDLTCLREVGLNPTRLFDTEL
                     AARLAGLARVGLGAVVEDLLGIHLAKEHSAADWSTRPLPQAWLVYAALDVELLVDLRE
                     ALARVLDDAGKTDIWPGYAGLML"
     gene            complement(608..1204)
                     /locus_tag="PLW15_11250"
     CDS             complement(608..1204)
                     /locus_tag="PLW15_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HOT35010.1"
                     /translation="MPDTPAGPTPPAVFTSAVESLRSATLRPELTVTEIPAPGTLAPW
                     SFALAADVTPARHGRDSDLGTGRFVLLYDASSPDAWGGPFRVICFAQAPLETEMGVDP
                     FIAEVAWSWLVDALADRGAVYTAASGTATRILSTGFGELEAQGQGAQIELRASWSPYD
                     HDLAAHVEGWSELLCMLAGLPPAVEGVSLLSARRVGRE"
     gene            1282..>2078
                     /locus_tag="PLW15_11255"
     CDS             1282..>2078
                     /locus_tag="PLW15_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT35011.1"
                     /translation="MSSRQQPDAVAARVAAGIAIGAGGLIALGAIASAAVAVIFARTV
                     LTPPTKRAQDVRIRAVDDSTVTLSATIDSMTPGRYGLWFSEDTGHARVGEIVSFTTDT
                     VTRQLLQVDFGDLASATRGRLGSWFYLSPDELGLPYSDVAVPTELGPAPAWLIPSAAP
                     TDRWVIQVHGRAVSRPETLRAVPVFHDAGYTSLLISYRNDGDAPRTGDYRYGLGDTEW
                     RDVDAAMRYAIEHGARNLVLMGWSMGGATVLQALTRSSSADRVSGVIL"
BASE COUNT          326 a          695 c          726 g          331 t
ORIGIN      
        1 cagcatcagg ccggcgtaac cgggccagat gtcggtcttg cccgcgtcgt caagcacacg
       61 ggcaagagcc tcgcgcaggt cgacgagcag ttcgacgtcg agtgcggcat agaccagcca
      121 tgcctgggga agcggtctcg tcgaccagtc agccgcggag tgctccttgg cgaggtggat
      181 gccgaggagg tcctcgacga cggcgccgag ccccacgcgc gccaggcccg ccagccgggc
      241 cgccagctcc gtgtcgaaca gcctggtcgg gttcagcccg acctcacgca gacaggtgag
      301 atcctggctc gccgcgtgca ggatccactc ctcgtgcgcg atcacgtcgt tgagctcagt
      361 gaacccgccg atcgccggag gatcgaagag gaacgtgccg gcatccctcc ggaagatctg
      421 gatcagatag gcgcgctgcg agtagcgata gccggatgcc cgctcggcgt cgatcgcgat
      481 cggtccggtg cccgccgcga tggttcgcac cgcctcaagg tactcatctc gggtggcgat
      541 gacgtccacc tgcgcgtgct cggcttgctg cggcgccgac ggctcgggcg cggcatcctc
      601 gtcggggcta ttcacgtccc actcggcgag ctgacaacag gctcactccc tcgactgccg
      661 gcggaaggcc ggcaagcatg cacaacagtt cgctccagcc ttccacatgt gcggcgaggt
      721 cgtgatcgta ggggctccag gatgcccgga gttctatctg agcgccctgt ccctgtgcct
      781 ccagctcgcc gaagccggtc gagagaattc gcgtcgccgt tcccgacgct gcggtgtaga
      841 cggcgccacg gtcggcgagt gcgtcaacga gccacgacca cgccacttcg gcgatgaagg
      901 gatcgacgcc catctcggtc tccagcggag cctgcgcgaa gcagatgacc cggaacggac
      961 cgccccaggc gtcgggggaa ctggcgtcgt acaggagaac gaatcgcccg gttcccagat
     1021 cggagtctcg gccgtggcgg gcgggcgtga catccgctgc gagggcgaac gaccacggtg
     1081 cgagggttcc gggcgcggga atctcggtga ctgtcaactc cgggcgcagg gtggcgctgc
     1141 gaagtgactc aacggcagag gtgaagaccg ctggaggggt ggggcctgcg ggtgtgtcgg
     1201 gcacgtccca aacactaggc tgaccgcggg gtttctccac agaggcacgc cgctggatgc
     1261 ggcgagcaga gggggttcgc gttgtccagt cgccagcaac cggatgccgt cgccgcacgg
     1321 gttgccgcag gcatcgccat cggtgccggg ggactcatcg cgctgggcgc catcgcgtcg
     1381 gcggcagtcg ccgtgatctt cgcccgcaca gtgctgaccc cgcccaccaa gagggcgcaa
     1441 gacgtccgca tccgcgccgt cgacgactcc acggtgaccc tctccgccac tatcgactcg
     1501 atgacacccg gccgttacgg tctgtggttc tccgaggaca ccggccatgc tcgggtcggc
     1561 gagatcgtgt cgttcacgac cgacaccgtc acccgtcagc tgctccaggt cgacttcggc
     1621 gacctcgcgt cagcgacgcg gggaaggctc gggagttggt tctacctgtc gcctgacgag
     1681 ctgggtctgc cttattccga cgtagcggtg ccgaccgagc tggggccggc gccagcgtgg
     1741 ctcattccct cggcggctcc caccgatcgg tgggtcatcc aggtgcatgg ccgcgcggtg
     1801 agccgacccg agacgctgcg tgccgttccg gtgttccacg acgccgggta cacctcgctg
     1861 ctcatctcgt accgcaacga cggcgacgcg ccgcgcaccg gcgactaccg ctacgggctc
     1921 ggtgacaccg agtggcggga tgtcgacgcg gccatgcggt acgcgatcga gcacggtgct
     1981 cggaacctgg tgctgatggg atggtcgatg gggggagcga cggtgcttca ggcactcacg
     2041 cggtcgtcct cggcggatcg ggtgagcggc gtcatcct
//
LOCUS       DAOSOU010000274         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_55716_length_2022_cov_4.179461, whole genome shotgun sequence.
ACCESSION   DAOSOU010000274 DAOSOU010000000
VERSION     DAOSOU010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55716_length_2022_cov_4.179461"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLW15_11260"
     CDS             complement(<1..512)
                     /locus_tag="PLW15_11260"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005273889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOT35012.1"
                     /translation="MLKVFGVGGLPEFVAGDDLAQILGDALEGVVEAGDILAVTSKVV
                     SKVEGRQVLAADREDAITAETVRVVATRVHPGGTTRIVENRLGMVLAAAGVDASNTPE
                     GVVLLLPEDPDASALRLCTALRERFGVSIGVLVTDTLGRPWRAGQTDVAIGAAGIRVM
                     DDLRGTTDASG"
     gene            complement(525..911)
                     /locus_tag="PLW15_11265"
     CDS             complement(525..911)
                     /locus_tag="PLW15_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35013.1"
                     /translation="MNQTDAAGNRTGPWQEPDPHGGVIEGEYVDGLRVGRWVHRFADG
                     RVRSEMEYVAGELTGPVTWYRSTGGLLQKGEFVDDEKHGFWQRWTAAGVLIDEGEFDH
                     GTKTGEWVYYAPDGSVKKRTVHRGRA"
     gene            complement(922..1932)
                     /locus_tag="PLW15_11270"
     CDS             complement(922..1932)
                     /locus_tag="PLW15_11270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOT35014.1"
                     /translation="MPRASTPHIGYAAMLERFPPREVVELATLAEQHGFTGVMATDHF
                     QPWTPQQGQASFVWNILSALGQTTAGDLGTGVTAPTFRSHPALTAQASATLAALYPGR
                     HWLGIGSGEALNEHIAGQYWPEAPERIDRMFEAVDVIKKLFASGLAQRDVRHDGQYFK
                     LESTRLWTMPERAPEILVATSGPVTARRAGRTVDGMITEVAPLDKIALLLQRFAEGAK
                     EAGRDPKTQVRVLRLHLSWAPTDEEAMANALAEWPNGGMRFGRSDIRSPFEFDQLAKL
                     VRPDDFEGRMLVTADLDAHRAHIQSFLDLGFDRIYLHNAGRNQAQWLEVFGRDVLPKL
                     TR"
BASE COUNT          293 a          769 c          650 g          310 t
ORIGIN      
        1 cccgaagcgt cggtcgtgcc ccgtaggtca tccatcaccc ggatgcccgc cgccccgatc
       61 gcgacatcgg tctgcccggc gcgccacggt cggcccaggg tgtcggtcac cagcacgccg
      121 atggacaccc cgaagcgctc ccgcagcgcg gtgcacaacc gcagggccga cgcatccgga
      181 tcctcgggca gcagcagcac gacgccctcg ggggtgttgc tcgcgtcgac gcccgccgcc
      241 gcaagcacca tgccgaggcg gttctccacg atgcgggtgg tgccgccggg gtgcacgcgc
      301 gtcgcgacga cgcgcacggt ttcggccgtg atcgcgtcct cgcggtcggc ggccagcacc
      361 tgccggccct ccaccttcga cacgaccttc gaggtcacgg ccagaatgtc gcccgcctcc
      421 accacgccct caagggcgtc gcccagaatc tgcgccaggt cgtcgcccgc aacgaactcc
      481 ggcagcccgc cgacgccgaa caccttgagc atcaccgaac cagcttaggc gcgccccctg
      541 tgcacggttc gcttcttcac gctgccgtcg ggcgcgtagt agacccactc accggtcttg
      601 gtgccgtggt cgaactcgcc ctcatcgatg agcacgcccg cggccgtcca gcgctgccag
      661 aagccgtgct tctcgtcgtc gacgaactcg cccttctgca gcagcccgcc cgtcgaccgg
      721 taccaggtga ccggcccggt gagctcgccc gccacgtact ccatctccga gcgcacgcgg
      781 ccatcggcga agcggtgcac ccagcggccc acgcgcaaac cgtcgacata ctcgccctcg
      841 atgaccccgc cgtgcgggtc gggctcctgc cacgggccgg tgcggttgcc cgcggcatcc
      901 gtctggttca tggcagcgac cctagcgcgt cagcttcggc agcacatccc ggccgaacac
      961 ctcaagccac tgggcctggt tgcgcccggc attgtgcagg tagatgcggt cgaacccgag
     1021 gtcgaggaag ctctggatgt gcgcccggtg cgcatcgagg tcggcggtga cgagcatccg
     1081 cccctcgaag tcgtcgggac gcaccagctt cgccaactgg tcgaactcga acggcgaacg
     1141 gatgtcgctg cgcccgaacc gcatgccgcc gttgggccac tcggccaggg cgttcgccat
     1201 cgcctcctcg tcggtggggg cccagctcag gtgcaggcgc agcacgcgca cctgcgtctt
     1261 cgggtcgcgg ccggcctcct tcgcgccctc cgcgaagcgc tgcagcagca gggcgatctt
     1321 gtcgagcggg gccacctcgg tgatcatgcc gtcgacggtg cggccggcgc ggcgggctgt
     1381 caccggtccg gatgtcgcga ccagaatctc gggcgccctc tcgggcatcg tccacaaccg
     1441 tgtcgactcc agcttgaagt actggccgtc gtggcggaca tcacgctggg cgagccccga
     1501 cgcgaacaac ttcttgatca cgtcgaccgc ctcgaacatg cggtcgatgc gctcgggcgc
     1561 ctccggccag tactgcccgg cgatgtgctc gttgagcgcc tccccgctgc cgatgcccag
     1621 ccagtgccgc cccggataca gcgccgccag cgtcgcactc gcctgagccg tgagcgccgg
     1681 atgcgaccgg aacgtcggcg ccgtcacccc ggtacccagg tctcccgctg tcgtctgccc
     1741 cagcgcactg aggatgttcc acacgaaact cgcctgcccc tgctgcggcg tccacggctg
     1801 gaagtggtca gtcgccatca cgccggtgaa cccgtgctgc tcggcgagcg tcgctagctc
     1861 aacaacctcc cgcggcggaa accgctcgag catggcggcg tagccgatgt ggggtgtgga
     1921 tgctcggggc atgagtacat tgtggcgcgg gtttcgggca ctcccgctac cgcagcatcg
     1981 cctccatcgc gacgacgagc cgatcggcgt gctggatcgc ct
//
LOCUS       DAOSOU010000275         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_57952_length_1965_cov_6.242932, whole genome shotgun sequence.
ACCESSION   DAOSOU010000275 DAOSOU010000000
VERSION     DAOSOU010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57952_length_1965_cov_6.242932"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="PLW15_11275"
     CDS             complement(<1..122)
                     /locus_tag="PLW15_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HOT35015.1"
                     /translation="MLPHDALWPRAGDWPAPAAGERADVAIIGMPTFRTSLSPT"
     gene            complement(122..1756)
                     /gene="hutU"
                     /locus_tag="PLW15_11280"
     CDS             complement(122..1756)
                     /gene="hutU"
                     /locus_tag="PLW15_11280"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOT35016.1"
                     /translation="MITAHRGSTLHTLGWPQEAALRMLQNNLDPEVAEHPESLVVYGG
                     TGKAARDWPSYHAIVRTLETLRGDETLLVQSGKPVGVMQTHEWAPRVLLANSNLVGDW
                     ANWDEFRRLEQLGLTMYGQMTAGSWIYIGTQGILQGTFETFSAVAEKKFGGTLAGTIT
                     LTAGLGGMGGAQPLAVTMNGGVAICVDVDASRISRRIQHGYLDVAADSLDHALELAYG
                     ARDARRPLSIGVLGNAASVVPELLARGAQIDIVTDQTSAHDPLAYLPLGVEFDEWETE
                     RGKEGFAQRARESMAVHVEAMVGFQRAGAEVFDYGNNIRTEALAAGYADAFAFPGFVP
                     AYIRPLFAEGKGPFRWAALSGEASDIAKTDRAVLELFPENRSLQRWIPLAQQRVHFQG
                     LPARICWLGYGERDVAGERFNDMVRSGELAAPIVIGRDHLDSGSVASPYRETEAMKDG
                     SDAIADWPLLNALVNTASGATWVSIHHGGGVGMGRSIHAGQVTVADGTALAGEKIARV
                     LTNDPGMGVIRHVDAGYDEAVAVADRLGVRVPMQEG"
     gene            complement(1753..>1965)
                     /locus_tag="PLW15_11285"
     CDS             complement(1753..>1965)
                     /locus_tag="PLW15_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOT35017.1"
                     /translation="VLAIELMTAARGIEMRGARPSTVSAAVIGALREVVPGPGPDRYL
                     ALDIQAAVDAVVNGTLLAASGIGATR"
BASE COUNT          295 a          711 c          642 g          317 t
ORIGIN      
        1 tgcgtcggcg agagcgacgt gcggaaggtc ggcataccga tgatggcgac atccgctcgc
       61 tcgcccgcgg ccggcgcggg ccagtcaccc gctcgcggcc agagtgcatc gtgcggcagc
      121 atcagccctc ctgcatcgga actctgacgc cgagcctatc cgcgacggcg accgcctcgt
      181 cgtagccagc atcgacgtgg cggatcacgc ccatgcccgg gtcattggtc agcactcggg
      241 cgatcttctc ccccgcgagc gccgtgccgt cggcgacggt cacctggccc gcgtgaatgg
      301 atcgacccat gccgacgccc ccgccgtgat ggatggagac ccaggtcgca cccgacgccg
      361 tgttgacgag ggcattcagc aatggccagt cggcgatggc atccgaaccg tccttcatcg
      421 cttcggtctc gcggtagggg ctggccaccg accccgagtc gaggtggtcg cgcccgatca
      481 cgatgggcgc ggcgagctcg cccgatctga ccatgtcatt gaaccgctcg ccagccacgt
      541 cacgctcgcc gtagcccagc cagcagatgc gcgcgggcaa cccctggaag tgcacgcgct
      601 gctgagccag cggaatccac cgctgcaggg atcgattctc ggggaacagc tccagcacgg
      661 ctcggtcggt cttcgcgatg tcgctcgcct ccccgctgag ggctgcccag cggaacggcc
      721 ccttgccctc cgcgaacagc gggcggatgt aggcgggcac gaaccccggg aaggcgaacg
      781 catccgcata ccccgcggcg agcgcctcgg tgcggatgtt gttgccgtag tcgaacacct
      841 cggcgcccgc gcgctggaat cccaccatcg cctcgacgtg caccgccatc gactcccgcg
      901 cgcgctgcgc gaacccctcc ttgccccgct cggtctccca ctcgtcgaac tcgaccccga
      961 gcggcagata ggccagcggg tcgtgggcac tcgtctgatc ggtgacgatg tcgatctggg
     1021 cgccccgcgc gagcagttcg ggcacgacgg atgccgcgtt gccgagcacg ccgatcgaca
     1081 ggggtcttct ggcatccctc gccccgtatg ccagctcgag cgcatgatcg aggctgtcgg
     1141 ccgcgacgtc gaggtagccg tgctggatgc ggcgcgagat gcggctcgcg tcgacatcca
     1201 cgcagatcgc aaccccgccg ttcatggtca ccgccagcgg ctgggcgcca cccatgccgc
     1261 ccagaccggc cgtcagcgtt atcgtgcccg cgagggtgcc cccgaacttc ttctcggcga
     1321 cggccgagaa ggtttcgaac gtgccctgaa gaatgccctg cgtgccgatg tagatccacg
     1381 accccgccgt catctggccg tacatcgtga ggcccagttg ctcgaggcga cggaactcgt
     1441 cccagttggc ccagtcgccc acgaggttcg agttggccag cagcacccgc ggtgcccact
     1501 cgtgggtctg catcacgccg accggcttgc cggactgcac gagcagtgtc tcgtcacccc
     1561 tgagcgtctc cagcgttctg acgatcgcgt gatagctcgg ccaatcgcgc gccgccttgc
     1621 cggtgccgcc gtagacgacg aggctctcgg gatgctcggc cacctcggga tcgaggttgt
     1681 tctgcagcat ccgcagagcc gcttcctgcg gccagccgag tgtgtgaagc gtcgagcctc
     1741 ggtgcgctgt gatcatcgtg ttgctccaat cccgcttgct gcgagcagtg ttccgttgac
     1801 gaccgcgtcg acggccgcct gaatgtcgag tgcgaggtac cgatcggggc ccggtccggg
     1861 cacgacctct cgcaaggcgc cgatgacggc cgcgctcacc gtcgacggtc gggcgccgcg
     1921 catctcgatg ccgcgggccg ccgtcatgag ctcgatggcg agcac
//
LOCUS       DAOSOU010000276         1931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_59421_length_1931_cov_5.343284, whole genome shotgun sequence.
ACCESSION   DAOSOU010000276 DAOSOU010000000
VERSION     DAOSOU010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59421_length_1931_cov_5.343284"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLW15_11290"
     CDS             <1..584
                     /locus_tag="PLW15_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT35018.1"
                     /translation="TEEAGVGQGTFYLYFASKLEIFEELVDDLNRRVRHAMSEASTGS
                     ATRLESERAGFRAFFAFTAEHPALYRIVREAEFVSPRALRSHYTRIVEGYISGLAEAR
                     EKGEVGDIDPTVAAWALMGIGEMIGMRWVLWGDGAPVGDNADPTASGTSEVPDAVFDE
                     MMRFIRRALAVAPEADGPAPADQTKPTVGRTQQ"
     gene            581..1324
                     /locus_tag="PLW15_11295"
     CDS             581..1324
                     /locus_tag="PLW15_11295"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HOT35019.1"
                     /translation="MTLDGRRALVTGGGSGIGAACAKALSGQGAHVIVADFNRESAER
                     VAEEVGGEPWVVDLGDTAALADLALDIDILVNNAGIQQVGPIETYDPAVFARIQRLMV
                     ESPFLLIRAALPAMYARGFGRIVNLSSVHGLRASPFKVAYVTAKHGLEGLSKVTALEG
                     GPHGVTSNCVNPGYVRTPLVEKQIADQAKVHGIPESEVVEKVMLTESAIKRLVEPDEV
                     ADLVAWLCGPTAGMVTGASYTMDGGWSAR"
     gene            1366..>1931
                     /locus_tag="PLW15_11300"
     CDS             1366..>1931
                     /locus_tag="PLW15_11300"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOT35020.1"
                     /translation="MPAINLGMPKVPEVLAPRRKTRQIHVGKVGVGSDHQVSVQSMTT
                     TQTTNINATLQQIAELTATGCDIVRVAVPHQDDADVLHIIAAKSQIPVIADIHFQPRY
                     VFTAIDAGVGAVRVNPGNIKKFDDQVGAIAKAAKAAGVSIRIGVNAGSLEPSILQKYG
                     KATAEALVESAVWEASLFEEHDFHDFKIS"
BASE COUNT          345 a          635 c          661 g          290 t
ORIGIN      
        1 tcaccgagga ggccggggtc gggcagggca cgttctatct gtacttcgcc agcaaactcg
       61 agatcttcga ggagctcgtc gacgacctga accggcgcgt tcgccacgcg atgagcgagg
      121 cctcgaccgg ttcggcgaca cgactcgagt cggagcgagc cggcttccgt gcgttcttcg
      181 ccttcacggc ggagcatccc gcgctgtacc gcatcgtgcg ggaggccgaa ttcgtgtcgc
      241 cccgcgcgct gcgatcgcac tacacccgca tcgtcgaggg ctacatctcc gggctcgcag
      301 aagctcgcga gaagggtgag gtcggcgaca tcgacccgac ggtcgcggcg tgggcgctca
      361 tgggcatcgg cgagatgatc gggatgcgct gggtgctctg gggcgacgga gccccggtgg
      421 gcgacaacgc agacccgacc gccagcggca cctccgaggt accggacgcg gtcttcgacg
      481 agatgatgcg attcatccgt cgtgcgctcg ccgtggcccc cgaggcggac gggccggcac
      541 ctgcagatca gacgaaaccg acggtcggaa ggacccagca atgacactcg acggtagacg
      601 tgccctcgtc acgggtggag gcagcggcat cggtgctgcc tgcgccaagg ccctctcggg
      661 tcagggtgcc cacgtgatcg tcgccgactt caatcgcgag tcggccgagc gcgtggccga
      721 ggaggtgggc ggcgagccct gggtcgtcga tctgggcgac accgcggcgc tcgcggattt
      781 ggcgctggac atcgacattc tcgtcaacaa cgccggcatc cagcaggtcg ggcccatcga
      841 gacctacgac ccggccgtgt tcgctcgcat ccagcgtctg atggtggagt cacccttcct
      901 gttgatccgc gcggcgctgc cggcgatgta cgcgcgcggc ttcgggcgga tcgtcaacct
      961 cagctcggtt cacgggctcc gcgcctcgcc gttcaaggtc gcctacgtca cggccaagca
     1021 cggtctcgag gggctgtcca aggtgaccgc gctcgagggc gggcctcacg gcgtcacgag
     1081 caactgcgtg aacccggggt acgtacgaac gcccctcgtc gagaagcaga tcgccgacca
     1141 ggcgaaggtg cacggcatac cggaatccga ggtcgtcgag aaggtcatgc tcacggagag
     1201 cgcgatcaag cggctggtgg aacccgatga ggtcgcagat cttgtcgcct ggctgtgcgg
     1261 gcccacggcc gggatggtca ccggcgcgtc gtacaccatg gacggcggct ggagcgcccg
     1321 gtgacgtcag ggccctgcca cctcccacgc acgtaaacta gaagcgtgcc agccatcaat
     1381 ctgggaatgc ccaaggttcc cgaagttctc gctccccgac gcaagacccg ccagatccac
     1441 gtgggcaagg tgggggtggg cagcgaccac caggtgagcg tgcagtcgat gacgacgacg
     1501 cagaccacga acatcaacgc gaccctgcag cagatcgccg aactgacggc caccggctgc
     1561 gacatcgtgc gagtggccgt gccccatcag gatgacgccg acgtgctgca catcattgcg
     1621 gcgaagagcc agatccccgt gatcgccgac atccacttcc agccgcgcta cgtgttcacg
     1681 gccatcgatg ccggcgtcgg tgccgtgcgc gtgaacccgg gcaacatcaa gaagttcgat
     1741 gaccaggtcg gggcgatcgc gaaggccgcg aaggccgcgg gcgtatcgat ccgcatcggc
     1801 gtcaacgcgg gatcactcga gccgagcatc ctgcagaagt acggcaaggc gaccgcggag
     1861 gcgctcgtcg agagcgcggt gtgggaggcc agcctcttcg aggagcacga cttccacgac
     1921 ttcaagatct c
//
LOCUS       DAOSOU010000277         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_60586_length_1904_cov_4.049757, whole genome shotgun sequence.
ACCESSION   DAOSOU010000277 DAOSOU010000000
VERSION     DAOSOU010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60586_length_1904_cov_4.049757"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLW15_11305"
     CDS             <1..294
                     /locus_tag="PLW15_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT35021.1"
                     /translation="AKGFDTFCPLGPVIETEFVVGSQAIETRMNGEVRQSGRLDEMVH
                     SVASVIAYASAVFTLLPGDVILTGTPAGVGPIVPGDTVEVTVEGIGTLGNPVR"
     gene            357..1832
                     /gene="gltX"
                     /locus_tag="PLW15_11310"
     CDS             357..1832
                     /gene="gltX"
                     /locus_tag="PLW15_11310"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT35022.1"
                     /translation="MSAPFSTATGTDVRVRFCPSPTGTPHVGLIRTALFNWAYARHTG
                     GTMVFRIEDTDAERDSEESYLQLLDALRWLKIDWDEGIDVGGPQAPYRQSQRTEIYRG
                     VIEQLLASGHLYESFATGEEIEARNVEQGRDPKQGYDNFERDLTPEQIAAYKAEGRQP
                     ALRLRVPDSDLSFTDLVRGEITFPAGSFTDFVVVRPNGAPLYTFVNPVDDALMGITHV
                     LRGEDLLSSTPRQIALYTALIEIGLTSFIPRFGHLPYVMGEGNKKLSKRDPESNLFHH
                     RDRGFIPEGLVNYLSLLGWSLSGDRDVFSLDEMVAAFDIVDVNPNPARFDVKKAESIN
                     GDHIRLLEVDDFARRLEPYLVGLVNNDDDRALLRRAAPLVQERMQLLGEAPGLLGFLF
                     ETDSQLVFEADALPGAEGAEVLDAALGALAGIPDWTTAGIEEALRAELIDSRGMKPRT
                     AFGPLRTAISGRRISPPLFESMELLGRESVLARITRLRAAL"
BASE COUNT          309 a          663 c          630 g          302 t
ORIGIN      
        1 gccaagggct tcgacacgtt ctgcccactc ggaccggtca tcgagaccga gttcgtcgtc
       61 ggcagccagg cgatcgagac gcggatgaac ggcgaggtgc gccagtcggg acggctcgac
      121 gagatggtgc actcggtagc atccgtcatc gcgtacgcct cggcggtgtt cactctgctg
      181 cccggcgacg tgatcctcac gggaaccccc gccggcgtcg gcccgatcgt gccaggcgac
      241 acggtcgagg tgaccgtcga gggcattgga accctgggca accccgtgcg ataggccggt
      301 tgagtagcgg cgaagccgcg tatcgaaacc gcccaccccg cctagacttg caggcgatgt
      361 ctgcaccctt ctccacagca accggaaccg acgtgcgcgt gcggttctgc ccctcgccga
      421 ccggaacgcc gcacgtcggc ctgatccgca cggccctctt caactgggcc tacgcgcgcc
      481 acacgggcgg cacgatggtc ttccgcatcg aagacaccga cgccgagcgc gacagcgagg
      541 agagctacct gcagctgctc gacgccctcc gctggctgaa gatcgactgg gacgagggca
      601 tcgacgtcgg tgggccgcag gcaccgtacc ggcagtcgca gcgcaccgag atctaccggg
      661 gcgtgatcga gcagcttctg gcatccggtc acctgtatga gtcgttcgcg accggcgagg
      721 agatcgaggc tcgcaacgtc gagcagggtc gcgaccccaa gcagggctac gacaacttcg
      781 agcgcgacct cacgcccgag cagatcgccg cctacaaggc ggagggccgc cagccggcgc
      841 tgcgtctgcg ggtgcccgac agtgacctga gcttcaccga cctggtgcgc ggcgagatca
      901 cgttcccggc aggctccttc accgacttcg tcgtggtgcg ccccaacgga gcaccgctct
      961 acacgttcgt caatcccgtg gatgacgcgc tcatgggcat cacgcatgtg ctgcgcggtg
     1021 aggacctcct gtcgtccacc ccccgtcaga tcgcgctcta cacggccctc atcgagatcg
     1081 gactcacgag tttcatcccc cggttcggac acctgccgta cgtgatgggg gagggtaaca
     1141 agaagctctc caagcgcgac ccggaatcca acctgttcca ccaccgcgac cgcgggttca
     1201 tccccgaggg tctcgtcaac tacctgtcgc tgctgggctg gtcgctgtcg ggcgaccgcg
     1261 acgtgttctc cctcgacgag atggtcgccg ccttcgacat cgtggacgtg aaccccaacc
     1321 cggcccgctt cgacgtgaag aaggccgagt cgatcaacgg cgaccacatc cgtctgctcg
     1381 aggtcgacga cttcgctcgg cggctggagc cgtacctcgt ggggctcgtg aacaacgacg
     1441 acgaccgcgc gctgctgcgc cgggccgcgc cgctcgtgca ggagcgcatg cagctgctgg
     1501 gggaggcgcc ggggctgctc gggttcctgt tcgagaccga ttcgcagctg gtcttcgagg
     1561 ccgatgcact gccgggcgcg gagggcgccg aggtgctcga tgccgcgctg ggcgcgctgg
     1621 cgggcatccc ggactggacg accgcgggca tcgaggaggc gctgcgtgcc gaactcatcg
     1681 acagccgggg catgaagccg cgcacggcct tcggcccgct gcgcaccgcg atctcgggtc
     1741 gccgcatcag cccgccgctc ttcgagtcga tggagctgct cggccgcgaa tccgtactcg
     1801 cgcgcatcac gaggctgcgc gcggcgctct gatcatggcc agaactgaat cgaggcgagc
     1861 cttccgcagc tggattccgg cgttagtgct ccttgtcgcg atcg
//
LOCUS       DAOSOU010000278         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_67554_length_1762_cov_6.011716, whole genome shotgun sequence.
ACCESSION   DAOSOU010000278 DAOSOU010000000
VERSION     DAOSOU010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67554_length_1762_cov_6.011716"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..579
                     /locus_tag="PLW15_11315"
     CDS             193..579
                     /locus_tag="PLW15_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35023.1"
                     /translation="MVLDLRSSELSSSDRLQSPSRHVGPKSSSTRLESRRDWLGIPAV
                     LDRALADPAAKHRLPPTMAVCAYGDLGGIVDNPLPARSDLGSVVPNGPSVAELTTSLL
                     GALERTGQKAVANEIREVLLRNGLPG"
     gene            complement(1206..1547)
                     /locus_tag="PLW15_11320"
     CDS             complement(1206..1547)
                     /locus_tag="PLW15_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT35024.1"
                     /translation="MVHTFEIEHAGAFDIGSVYQAHAQGRGADYSWVAFHDIGRLAGR
                     DAGAQRERARVVATATELGIGLVAFKKPNNLETWTTIDAMRRRPTPEERALFEDRVMP
                     RVPAVGVAVAE"
BASE COUNT          274 a          588 c          566 g          334 t
ORIGIN      
        1 aacgaacgcc gcgctgtgct cgcacttccc aaggctgcag taccgaccgg atccgcctcg
       61 acatcgccac tcgacgacta ccgcgaggtc ctccgcgccg tcgtgctctt gcctgcccga
      121 atcaagccgg gtagccgaga gttcatcgcg gtcgttcttc tcggcgcaga ggccgatccc
      181 gaccggaaca tcgtggtgct ggatctgcgc agcagcgagc tctccagttc ggaccgcctc
      241 cagagccctt cgcggcacgt cggtcccaag tcatcgtcga cccggctcga gtcacggcgt
      301 gactggctcg ggattccggc ggtgctggac cgggcgctcg ccgatcccgc tgccaaacat
      361 cggcttccac cgacgatggc cgtctgtgcg tatggtgacc tcggcggcat cgttgacaac
      421 ccgctgccgg ctcggagcga cctggggtcg gtcgtgccga atggcccttc ggtagccgag
      481 ctgacgacct cgctcctcgg cgcgttggag cggaccggtc agaaagccgt ggcgaacgag
      541 atacgggagg tgctccttcg gaatgggcta cccggatgag tcctctcgat gaacccggag
      601 tctcgaagat cgtgccgccc tccctcgagc tcgtcgcatt ttcgtcctgc ggtcgagcgg
      661 cgggtagttg cgttcggagt gtcgcagtcc gcggtgaatg tcgatcgccg gaaaccgaag
      721 cgagcggtgc cgctgagcga aggtggacaa ggtgggtttg acgttcattc cttcgactcc
      781 gatcccagcg tcggtcgatg gcctgacgga ttcgtattgg ttcgcagtgt gggaccggcc
      841 gattccggtg ccgtacgatc tgcatccggg cggaacggtg tacctccaag acagtgtcac
      901 cggcgtcatc gtgtgggaga cgacggtgac cgacatggtc gccgttccct acgagtcatc
      961 gcagatgctt cgcgacttct tgtcgtcgcg ctggggccct ggagcaagca ccattcgagg
     1021 atccgacccc tgcccggggt tctgcgttgc atggcgggcg aacgctgtcg agcgactcga
     1081 cctgacggtt ccggaaggac tggacccgct ggagtcgtgg acgtcgacgt accacctcga
     1141 ctgggacgaa cgggagcttc gtggccttcg ggacgaaggc ccgtgccccg tctgctgaac
     1201 cggactcact cagcgacggc tactccgacc gcaggtactc tcggcatcac tcggtcctcg
     1261 aacagagccc gctcctcagg tgtcggccgc cggcgcatgg catcgatggt cgtccaggtc
     1321 tcgaggttgt tcggtttctt gaacgccacg agaccgatgc cgagttccgt cgcggttgcg
     1381 acaacccgtg cgcgttcccg ctgcgctccg gcgtcccggc cggcgaggcg gccgatgtcg
     1441 tggaacgcaa cccacgagta gtccgccccg cgaccctgag cgtgggcctg atacaccgac
     1501 ccgatgtcga atgctccagc gtgttcgatc tcgaacgtgt ggaccacgat gttcggcgga
     1561 tctccagccg ttctgcgtcg ccgttgttcg cggagcacga cgtcggggtg cacccagatc
     1621 gatccatcgc ggcgcccgga ccgtgaagtg tcgaacgtct cgacgtgtcg catcccacgc
     1681 cgcgggcccc aaccttgctc cagcttgctg cgaaccactg cgtagatcgc tatctggcca
     1741 accgcacccg ttccctcgct ga
//
LOCUS       DAOSOU010000279         1629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoglobus sp. isolate AMR_MDS_2337
            NODE_75387_length_1629_cov_4.769377, whole genome shotgun sequence.
ACCESSION   DAOSOU010000279 DAOSOU010000000
VERSION     DAOSOU010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864404
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoglobus sp. (wastewater metagenome)
  ORGANISM  Rhodoglobus sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Rhodoglobus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:21:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,234
            Genes (coding)                    :: 2,217
            CDSs (with protein)               :: 2,217
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Rhodoglobus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75387_length_1629_cov_4.769377"
                     /isolate="AMR_MDS_2337"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1950123"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLW15_11325"
     CDS             complement(<1..321)
                     /locus_tag="PLW15_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOT35025.1"
                     /translation="MTERTLIKNLLAATDGPVSVSGWVDTVRDQKKVQFVVLRDESGA
                     VQLVNPRASDADGNTIADELSDTISGLAQGTFLTATGELKHDERVKLGGVEIKLEALE
                     IAAAA"
     gene            complement(331..597)
                     /locus_tag="PLW15_11330"
     CDS             complement(331..597)
                     /locus_tag="PLW15_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT35026.1"
                     /translation="MSGYRIELRPAAIKALRAIERKDRARIQGAIALLGENPRPPGAR
                     ALHGRDGLRVRVGDYRIIYTIRDDVLLVVVVTLGHRKDVYSPRN"
     gene            complement(594..842)
                     /locus_tag="PLW15_11335"
     CDS             complement(594..842)
                     /locus_tag="PLW15_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOT35027.1"
                     /translation="MSKISVTDARGRLPELIDRARTEAVFLERRGKLEAVVLSPEQYE
                     RMMDALEEVEDVAAFDEAMAEEGDSIPWAQVKADLGWT"
     gene            921..1550
                     /locus_tag="PLW15_11340"
     CDS             921..1550
                     /locus_tag="PLW15_11340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT35028.1"
                     /translation="MPADLIHLVRHGEVHNPEGVLYGRIPGYHLSELGHRMADAAAAS
                     LAGHPVTRLYASPLQRAQESAAPWSERFGLPITTDDRLIEPHNWFEGKKMREALKRPS
                     GWIQLRNPMKPSWGEPFISVRDRMLAVVTDAWRDTDSGEAVLVSHQMPIVMVARSVKD
                     LRLPHDPRDRRCALSSITTLERRGDAFVEVNYQEPAKDLGQAAIDLGAV"
BASE COUNT          268 a          543 c          523 g          295 t
ORIGIN      
        1 ggctgcggcg gcgatctcga gtgcctcgag cttgatctcc accccgccga gcttcacgcg
       61 ctcgtcgtgc ttgagctcac cggtcgcggt gaggaaggtg ccctgggcga gacccgagat
      121 ggtgtcactg agttcgtcgg cgatggtgtt gccgtcggcg tcggatgcac gagggttcac
      181 caactgcaca gcaccggact cgtcgcggag cacgacgaac tgcaccttct tctggtcgcg
      241 caccgtatcg acccaccccg acacggagac gggtccgtcg gttgcggcga gcaggttctt
      301 gatcagggtg cgttcggtca cccccagagt ttagttgcgt ggcgagtaga catctttgcg
      361 atgtccgaga gtaaccacca ctacaagcag cacgtcgtcg cggatcgtgt agatgatccg
      421 atagtcgccc acccgaacgc ggaggccatc acggccgtga agtgctcgtg cgccgggcgg
      481 acgggggttc tctcccagca gggcgatcgc cccttggatt ctggcgcgat ccttgcgctc
      541 gatggcgcgc agagccttga tcgccgccgg acgcagctcg atgcggtagc cgctcacgtc
      601 cagccgaggt ctgccttgac ctgagcccag ggaatgctgt cgccttcctc cgccattgcc
      661 tcgtcaaatg cggcgacgtc ttccacttct tccagcgcgt ccatcatgcg ctcatactgc
      721 tcggggctca gcaccactgc ctcgagtttt ccacggcgct caagaaagac cgcctcggtg
      781 cgcgcccgat cgatcaattc ggggaggcgg ccgcgggcgt ccgtcacgct gatcttcgac
      841 acttctccaa tgtacatgtg acgtcgtcga atgtacattg cggcgccagc gggctcgatg
      901 gcgagccgta gaatcgaggg gtgcccgccg acctcatcca tctcgtgcgc cacggtgagg
      961 ttcacaaccc cgagggcgtt ctctacggtc gcatcccggg ctaccacctg agcgaactgg
     1021 gccaccgcat ggcggatgcc gcggcggcga gtctggcggg gcatccggtc acccggctct
     1081 acgccagccc actccagcgc gcgcaggagt ctgccgcccc ctggtcggag cgtttcgggc
     1141 taccgatcac gaccgacgac cgcctcatcg agccgcacaa ctggttcgag ggcaagaaga
     1201 tgcgcgaggc gttgaagcgc ccctcgggct ggatccagct gcgcaatccc atgaagccga
     1261 gttggggcga gccgttcatc agcgtgcgtg accgcatgct cgcggtcgtc accgacgcgt
     1321 ggcgcgacac cgacagcggg gaagccgtgc tcgtcagcca ccagatgccg atcgtcatgg
     1381 tggcccgctc ggtcaaggat ctgcggctgc cgcacgaccc gcgcgatcgg cgctgcgcgc
     1441 tctccagcat cacgacgctc gagcgtcggg gcgatgcctt cgtcgaggtc aactatcagg
     1501 aacctgcgaa agacttaggg caagcagcaa tcgacttggg agcagtgtga ggttccgact
     1561 tcttgcggcg gccggagctc tcgtgctcgt cctctcgggg tgcaccaacg atccgctcgc
     1621 cgagcagta
//
